POPTR_0001s21200g cDNA ORF clone, Populus trichocarpa(Populus balsamifera subsp. trichocarpa)

Gene
Clones
gRNAs

Summary

Gene Symbol	POPTR_0001s21200g
Entrez Gene ID	7470541
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024593683.1	XP_024449451.1	mediator of RNA polymerase II transcription subunit 15a isoform X1
XM_024593682.1	XP_024449450.1	mediator of RNA polymerase II transcription subunit 15a isoform X1
XM_024593685.1	XP_024449453.1	mediator of RNA polymerase II transcription subunit 15a isoform X1
XM_024593686.1	XP_024449454.1	mediator of RNA polymerase II transcription subunit 15a isoform X2
XM_024593687.1	XP_024449455.1	mediator of RNA polymerase II transcription subunit 15a isoform X3
XM_024593688.1	XP_024449456.1	mediator of RNA polymerase II transcription subunit 15a isoform X4
XM_002298170.2	XP_002298206.1	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome of black cottonwood, Populus trichocarpa (Torr. & Gray).
Tuskan GA, Difazio S, Jansson S, Bohlmann J, Grigoriev I, Hellsten U, Putnam N, Ralph S, Rombauts S, Salamov A, Schein J, Sterck L, Aerts A, Bhalerao RR, Bhalerao RP, Blaudez D, Boerjan W, Brun A, Brunner A, Busov V, Campbell M, Carlson J, Chalot M, Chapman J, Chen GL, Cooper D, Coutinho PM, Couturier J, Covert S, Cronk Q, Cunningham R, Davis J, Degroeve S, D?jardin A, Depamphilis C, Detter J, Dirks B, Dubchak I, Duplessis S, Ehlting J, Ellis B, Gendler K, Goodstein D, Gribskov M, Grimwood J, Groover A, Gunter L, Hamberger B, Heinze B, Helariutta Y, Henrissat B, Holligan D, Holt R, Huang W, Islam-Faridi N, Jones S, Jones-Rhoades M, Jorgensen R, Joshi C, Kangasj?rvi J, Karlsson J, Kelleher C, Kirkpatrick R, Kirst M, Kohler A, Kalluri U, Larimer F, Leebens-Mack J, Lepl? JC, Locascio P, Lou Y, Lucas S, Martin F, Montanini B, Napoli C, Nelson DR, Nelson C, Nieminen K, Nilsson O, Pereda V, Peter G, Philippe R, Pilate G, Poliakov A, Razumovskaya J, Richardson P, Rinaldi C, Ritland K, Rouz? P, Ryaboy D, Schmutz J, Schrader J, Segerman B, Shin H, Siddiqui A, Sterky F, Terry A, Tsai CJ, Uberbacher E, Unneberg P, Vahala J, Wall K, Wessler S, Yang G, Yin T, Douglas C, Marra M, Sandberg G, Van de Peer Y, Rokhsar D
Science (New York, N.Y.)313(5793)1596-604(2006 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following POPTR_0001s21200g gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POPTR_0001s21200g cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPa00828	XM_002298170.2 Latest version!	Populus trichocarpa hypothetical protein (POPTR_0001s21200g) mRNA, complete cds.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OPa423153	XM_024593685.1 Latest version!	Populus trichocarpa mediator of RNA polymerase II transcription subunit 15a (LOC7470541), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OPa423153	XM_024593682.1 Latest version!	Populus trichocarpa mediator of RNA polymerase II transcription subunit 15a (LOC7470541), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OPa423153	XM_024593683.1 Latest version!	Populus trichocarpa mediator of RNA polymerase II transcription subunit 15a (LOC7470541), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OPa423154	XM_024593686.1 Latest version!	Populus trichocarpa mediator of RNA polymerase II transcription subunit 15a (LOC7470541), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OPa423155	XM_024593687.1 Latest version!	Populus trichocarpa mediator of RNA polymerase II transcription subunit 15a (LOC7470541), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OPa423156	XM_024593688.1 Latest version!	Populus trichocarpa mediator of RNA polymerase II transcription subunit 15a (LOC7470541), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POPTR_0001s21200g ( XM_002298170.2 ) cDNA ORF clone, Populus trichocarpa(Populus balsamifera subsp. trichocarpa) -> XP_002298206.1 Populus trichocarpa hypothetical protein (POPTR_0001s21200g) mRNA, complete cds.

Price & Availability↑

CloneID	OPa00828
Clone ID Related Accession (Same CDS sequence)	XM_002298170.2
Accession Version	XM_002298170.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2319bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2013-12-30
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_008467). On Dec 16, 2013 this sequence version replaced XM_002298170.1. COMPLETENESS: incomplete on the 5' end.

ATGAAATCTG CTCTAATTTA TATCAAAGTC ATGAAGGAGA TGTATTTCCC TGAAATAAAT 
GAAATATACC AGAGAATTGC TGCCAAGTTA CCGCAGCATG ACTCTCATCC GCAACAACCA 
AAGTCAGAAC AGCTTGACAA GCTAAAGGCA CTTAAGACCA TGTTGGAGCG CCTGATAATG 
TTCTTACAAG TTCCGAAAAA CAACATCAAG CTTAATTTCA AAGAGAAGTT GGGTTACTAT 
GAGAATCAGA TTTTAAATTT TCTAAACACA AGCAGACCCA GAAAGCCTGT TCCTAATCTT 
CAGCAAGGAC AGCTTCCCCA GCTTCATATG CAACCTATGC AGCGACCCCA ATCTCAAGTT 
CCTCAATTGC AGTCCCATGA AAATCAACTC AACCCCCAGT TGCAATCAAT GAATTTGCAA 
GGTTCTGTAC CAACAATGCA GCAGAACAAT GTGCCAAGCT TGCCGCATAA TTCTCTTTCT 
TCTTTATCTG GGGTTTCTAC ATCACAACCA AACAAGATGA ATCCAATGCA ATCAGCTTCC 
AATTTAGATT CTGGACAAGG AAATTCACTG AGCTCATTGC AGCAGGCACC TGTAGGATCT 
GTACAACAGA ATCCTGTGAG CAGTTCCCAA CCTACCAATT TTAACACATT GTCAACACAA 
AGTGGGGTGA GTATGCTGCA GTCGAATATT CCTCTTCAGT TGAATTCCAA TATGATTCAA 
CAGCAGCATC TAAAACAGCA GATGCTACAA ACACAACAGC TTAAACAACA GTTCCAACAG 
CGTCAGCCGC AGCAGCAACA GCGTCAGACG CAGCAGCAAC AGCATTTAAT GCAGAAGCAA 
CAGATGCTGC TGCAGCAGCA GCAGCAGCAG TTACACCAGC AAGCCAAGCA GCAGTTGCCT 
GCACAGATGC AGACACAACA AATTCCACAG CCGCATCAGA TGAATGATGT AAATGAGATG 
AAGTTGAGAC AGGGGATTGG AATCAAGCCA GCAGTCTTCC AACAACATCT CTCGACAGGT 
CAACGCACTG CTTTCCCTCA TCAACAAATG AAACCAGGAT CCTCATTTCC TATTTCTTCA 
CCACAAATGC TCCAGCATGC TTCCCCCCAA TTACAACAAC ATTCTTCTCC TCAGATTGAC 
CAGCAAAATC TACTGCCTTC TCTAACTAAA ACTGGAACAC CTTTGCAATC TGCAAACTCG 
CCTTTTGTTG TCCCATCTCC TTCAACTCCT TTAGCTCCAT CCCCTGTGCC AGGAGATTCT 
GAGAAACCCA TTTCTGGTAT CTCCTCAAAT ATTGTACACC AACCAACTGT TGCACAAGCA 
ACGGCTCCAT CCCTTGCAAT TGGCACTCCT GGGATATCTG CCTCGCCTTT GCTTGCAGAG 
TTTACCAGTC CTGATGGTGC TCATGGTGGT GCTTTGACAA CTGTTTCTGG CAAGTCGAAT 
GTTATGGAGC AACCACTTGA ACGCATGATT AAAGCGGTGA AATCTCTTTC TCCTAAAGCA 
CTTAGTGCAT CTGTCAGTGA CATTGGCTCT GTTGTCAGTA TGATTGACAG AATTGCTGGC 
TCGGCACCCG GTAATGGGTC TAGAGCTGCA GTTGGTGAGG ATCTGGTAGC GATGACAAAA 
TGTCGCCTTC AGGCAAGAAA TTTCTTCACC CAAGATGGGA TGACTGGATC GAAGAAAATG 
AGGCGCCATA CAAGTGCCAT GCCCTTGAAT GTTGCATCAT CAGCTGGCAG TGTAAGCGAC 
AGTTTTAAGC AGTTTACTGG TCCAGAGACA TCTGATTTGG AGTCGACTGT AACGTCAAGT 
GTCAAGAGGC CTAAAATTGA GGCTAATCAT GCCCTTTTGG AAGAGATAAG GGAAATAAAT 
CAGCGACTTT TAGACACAGT GGTTGATATT AGTGATGAAG ATGTTGACTC AACTGCAGCA 
GCAGCTGCTG CTGAAGGGGG TGGAGGAACC TTTGTCAAAT GCTCATTTAT CGCTGTGGCT 
CTGAGTCCAA ACTTGAAAGC ACAGTACACT TCAGCGCAAA TGTCAACAAT TCAGCCCCTT 
AGATTGCTTG TGCCTACAAA TTATCCGAAT TGCTCACCGA TACTGTTAGA CAAGTTTCCA 
TTTGAAGTCA GTAAAGAGTA CGAGGATTTG TCAATAAAAG CTAAATTTAG GTTTAGCATC 
TCTCTTCGAA GCCTTTCACA GCCCATGTCG CTTGGAGACA TAGCCAGGAC TTGGGATGTC 
TGTGCCCATG CTGTTATTTC AGAGCATGCA CAACAAAGTG GCGGAGGCAC CTTCAGCTCA 
AAATATGGTT CTTGGGAAAA CTGCGTGAGT GCAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002298206.1
CDS	1..2319
Translation	MKSALIYIKVMKEMYFPEINEIYQRIAAKLPQHDSHPQQPKSEQLDKLKALKTMLERLIMFLQVPKNNIKLNFKEKLGYYENQILNFLNTSRPRKPVPNLQQGQLPQLHMQPMQRPQSQVPQLQSHENQLNPQLQSMNLQGSVPTMQQNNVPSLPHNSLSSLSGVSTSQPNKMNPMQSASNLDSGQGNSLSSLQQAPVGSVQQNPVSSSQPTNFNTLSTQSGVSMLQSNIPLQLNSNMIQQQHLKQQMLQTQQLKQQFQQRQPQQQQRQTQQQQHLMQKQQMLLQQQQQQLHQQAKQQLPAQMQTQQIPQPHQMNDVNEMKLRQGIGIKPAVFQQHLSTGQRTAFPHQQMKPGSSFPISSPQMLQHASPQLQQHSSPQIDQQNLLPSLTKTGTPLQSANSPFVVPSPSTPLAPSPVPGDSEKPISGISSNIVHQPTVAQATAPSLAIGTPGISASPLLAEFTSPDGAHGGALTTVSGKSNVMEQPLERMIKAVKSLSPKALSASVSDIGSVVSMIDRIAGSAPGNGSRAAVGEDLVAMTKCRLQARNFFTQDGMTGSKKMRRHTSAMPLNVASSAGSVSDSFKQFTGPETSDLESTVTSSVKRPKIEANHALLEEIREINQRLLDTVVDISDEDVDSTAAAAAAEGGGGTFVKCSFIAVALSPNLKAQYTSAQMSTIQPLRLLVPTNYPNCSPILLDKFPFEVSKEYEDLSIKAKFRFSISLRSLSQPMSLGDIARTWDVCAHAVISEHAQQSGGGTFSSKYGSWENCVSAA

Target ORF information:

RefSeq Version	XM_002298170.2
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)
Definition	Populus trichocarpa hypothetical protein (POPTR_0001s21200g) mRNA, complete cds.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002298170.2

ORF Insert Sequence:

ATGAAATCTG CTCTAATTTA TATCAAAGTC ATGAAGGAGA TGTATTTCCC TGAAATAAAT 
GAAATATACC AGAGAATTGC TGCCAAGTTA CCGCAGCATG ACTCTCATCC GCAACAACCA 
AAGTCAGAAC AGCTTGACAA GCTAAAGGCA CTTAAGACCA TGTTGGAGCG CCTGATAATG 
TTCTTACAAG TTCCGAAAAA CAACATCAAG CTTAATTTCA AAGAGAAGTT GGGTTACTAT 
GAGAATCAGA TTTTAAATTT TCTAAACACA AGCAGACCCA GAAAGCCTGT TCCTAATCTT 
CAGCAAGGAC AGCTTCCCCA GCTTCATATG CAACCTATGC AGCGACCCCA ATCTCAAGTT 
CCTCAATTGC AGTCCCATGA AAATCAACTC AACCCCCAGT TGCAATCAAT GAATTTGCAA 
GGTTCTGTAC CAACAATGCA GCAGAACAAT GTGCCAAGCT TGCCGCATAA TTCTCTTTCT 
TCTTTATCTG GGGTTTCTAC ATCACAACCA AACAAGATGA ATCCAATGCA ATCAGCTTCC 
AATTTAGATT CTGGACAAGG AAATTCACTG AGCTCATTGC AGCAGGCACC TGTAGGATCT 
GTACAACAGA ATCCTGTGAG CAGTTCCCAA CCTACCAATT TTAACACATT GTCAACACAA 
AGTGGGGTGA GTATGCTGCA GTCGAATATT CCTCTTCAGT TGAATTCCAA TATGATTCAA 
CAGCAGCATC TAAAACAGCA GATGCTACAA ACACAACAGC TTAAACAACA GTTCCAACAG 
CGTCAGCCGC AGCAGCAACA GCGTCAGACG CAGCAGCAAC AGCATTTAAT GCAGAAGCAA 
CAGATGCTGC TGCAGCAGCA GCAGCAGCAG TTACACCAGC AAGCCAAGCA GCAGTTGCCT 
GCACAGATGC AGACACAACA AATTCCACAG CCGCATCAGA TGAATGATGT AAATGAGATG 
AAGTTGAGAC AGGGGATTGG AATCAAGCCA GCAGTCTTCC AACAACATCT CTCGACAGGT 
CAACGCACTG CTTTCCCTCA TCAACAAATG AAACCAGGAT CCTCATTTCC TATTTCTTCA 
CCACAAATGC TCCAGCATGC TTCCCCCCAA TTACAACAAC ATTCTTCTCC TCAGATTGAC 
CAGCAAAATC TACTGCCTTC TCTAACTAAA ACTGGAACAC CTTTGCAATC TGCAAACTCG 
CCTTTTGTTG TCCCATCTCC TTCAACTCCT TTAGCTCCAT CCCCTGTGCC AGGAGATTCT 
GAGAAACCCA TTTCTGGTAT CTCCTCAAAT ATTGTACACC AACCAACTGT TGCACAAGCA 
ACGGCTCCAT CCCTTGCAAT TGGCACTCCT GGGATATCTG CCTCGCCTTT GCTTGCAGAG 
TTTACCAGTC CTGATGGTGC TCATGGTGGT GCTTTGACAA CTGTTTCTGG CAAGTCGAAT 
GTTATGGAGC AACCACTTGA ACGCATGATT AAAGCGGTGA AATCTCTTTC TCCTAAAGCA 
CTTAGTGCAT CTGTCAGTGA CATTGGCTCT GTTGTCAGTA TGATTGACAG AATTGCTGGC 
TCGGCACCCG GTAATGGGTC TAGAGCTGCA GTTGGTGAGG ATCTGGTAGC GATGACAAAA 
TGTCGCCTTC AGGCAAGAAA TTTCTTCACC CAAGATGGGA TGACTGGATC GAAGAAAATG 
AGGCGCCATA CAAGTGCCAT GCCCTTGAAT GTTGCATCAT CAGCTGGCAG TGTAAGCGAC 
AGTTTTAAGC AGTTTACTGG TCCAGAGACA TCTGATTTGG AGTCGACTGT AACGTCAAGT 
GTCAAGAGGC CTAAAATTGA GGCTAATCAT GCCCTTTTGG AAGAGATAAG GGAAATAAAT 
CAGCGACTTT TAGACACAGT GGTTGATATT AGTGATGAAG ATGTTGACTC AACTGCAGCA 
GCAGCTGCTG CTGAAGGGGG TGGAGGAACC TTTGTCAAAT GCTCATTTAT CGCTGTGGCT 
CTGAGTCCAA ACTTGAAAGC ACAGTACACT TCAGCGCAAA TGTCAACAAT TCAGCCCCTT 
AGATTGCTTG TGCCTACAAA TTATCCGAAT TGCTCACCGA TACTGTTAGA CAAGTTTCCA 
TTTGAAGTCA GTAAAGAGTA CGAGGATTTG TCAATAAAAG CTAAATTTAG GTTTAGCATC 
TCTCTTCGAA GCCTTTCACA GCCCATGTCG CTTGGAGACA TAGCCAGGAC TTGGGATGTC 
TGTGCCCATG CTGTTATTTC AGAGCATGCA CAACAAAGTG GCGGAGGCAC CTTCAGCTCA 
AAATATGGTT CTTGGGAAAA CTGCGTGAGT GCAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POPTR_0001s21200g ( XM_024593683.1 ) cDNA ORF clone, Populus trichocarpa(Populus balsamifera subsp. trichocarpa) -> XP_024449451.1 Populus trichocarpa mediator of RNA polymerase II transcription subunit 15a (LOC7470541), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPa423153
Clone ID Related Accession (Same CDS sequence)	XM_024593683.1 , XM_024593682.1 , XM_024593685.1
Accession Version	XM_024593683.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3912bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-08
Organism	Populus trichocarpa(black cottonwood)
Product	mediator of RNA polymerase II transcription subunit 15a isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037285.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCCA ATAATTGGAG GCCTACTGCC CCCGGTGGAG AACCTGTCAT GGACACGGGT 
GATTGGAGAA CTCAATTGCA GCCGGACGCA CGCCAAAGAA TAGTCAACAA AATCATGGAG 
ACATTAAAAA GACATCTTCC ATTTTCTGGT CAAGAGGGAT TACAAGAACT CAAGAAAATA 
GCCGTACGGT TTGAGGAAAA GATTTATACT GCTGCAACAA GTCAGTCTGA TTATCTGCGT 
AAAATATCTC TGAAGATGCT TGCAATGGAG AACAAGTCTC AAAGTACCAT ACCCAATTCT 
TTGCCACCCA ACTCCACTGG CAGTGGTAAC AAACCCCTGG ATCCAGGAGG ATCTCACAGT 
ATGCAGTCTC AAGTTCACAA TCAAGGGCCA TCACTCACTA TCCCATTGCC TGCTAATCAA 
TCTCAAGAAC GCCAGCAATT GTTGTCACAG AACATGCAGA CTGGTATGGC ATCTAGTGGA 
GTTCAAAGTT CTTCTGGTTT AACGTCAGCA TTGCCTCCCA TCTCCAGTTT AACCCAGACT 
GTCCCCAACT CTGTTGGCCA GAATCCTAAC ATGCAGAGTA TCTCTGGTGT TTCACAGAAC 
CCAGTGGGGA ATCCAATGGG ACAAGGGGTG CCCTCCAATA TGCTTGCCAA TTCTCAGAGA 
CAAGGTAGGC AACTAGTTGT TCCACAGCAA CAACAGCAGC CCCAAAATCC TCAGCAGTAT 
TTTTACCAGC AGCAGTTACA ACAACAGCTT TTGAAGCAGA AGCTTCAACA GGGAAATCCC 
CAGCACTCAC TTGTGCAATC TCACGTCCAG CAGCAGCAAC AGCAGCAGCA AAACCTATTA 
CAGTCAAATC AGTTGCAATC TTCTCAGCAG TCTGGTGTGC AAACACCATC TGTTATGCAG 
CCTTCAATAA TGCAAACGGC TCCTCTCTCT TGTCTTCAAC AGAATCAGCC ATCTTCTGTT 
CAACAGTCAA CACAACCCAT GCTTCAGCAA CATCCACAAC CAGTCCTCAG GCAGCAGCAA 
CAACCTCAAC AGACTGCTGG TATTCATCAG CAGCAGAGGT TGCTAGGCCA GCAGAATAAC 
CTTCCAAATC TGCAGCAGCA GCAATTAATG GTTCAACAAA ATAACCTATC AAGCATGCAT 
CAGCAACAGC TGGGCTCTCA AAGTAATGTC TCCAGTTTAC AGCAGCAGCA GCTGCTTGGA 
GCTCAGTCTG GTAACTCAAG CATGCAAACT AATCAGCACC CTGTACACAT GCTACAACAG 
TCCAAGGTTA CGCTGCAGCA ACAAGCACAA CAAAGTGCAG GTACTTTGTT ACCTAATCAA 
GGGCAGCAGT CACAACCACA GCTGCCACAG CAGCAATTGA TGTCACAGAT CCAATCACAG 
CCAGTGCAGC TGCAACAGCA ATCAAATCCA TTACAACATG ATCTGCAACA AAGATTTCAA 
GCATCAGGTT CCTTGCTTCA ACAGCAGAAT GTGACTGATC AGCAAAAGCA GTTATATCAA 
TCTCAAAGAG CCCTTCCAGA AACGTCCCCA ACATCCCTAG ATTCTACAAC ACAGACTGGA 
CATGTGAATG TCAATGATTG GCAAGAGGAG ATCTACCAAA AGATCAAAGT CATGAAGGAG 
ATGTATTTCC CTGAAATAAA TGAAATATAC CAGAGAATTG CTGCCAAGTT ACCGCAGCAT 
GACTCTCATC CGCAACAACC AAAGTCAGAA CAGCTTGACA AGCTAAAGGC ACTTAAGACC 
ATGTTGGAGC GCCTGATAAT GTTCTTACAA GTTCCGAAAA ACAACATCAA GCTTAATTTC 
AAAGAGAAGT TGGGTTACTA TGAGAATCAG ATTTTAAATT TTCTAAACAC AAGCAGACCC 
AGAAAGCCTG TTCCTAATCT TCAGCAAGGA CAGCTTCCCC AGCTTCATAT GCAACCTATG 
CAGCGACCCC AATCTCAAGT TCCTCAATTG CAGTCCCATG AAAATCAACT CAACCCCCAG 
TTGCAATCAA TGAATTTGCA AGGTTCTGTA CCAACAATGC AGCAGAACAA TGTGCCAAGC 
TTGCCGCATA ATTCTCTTTC TTCTTTATCT GGGGTTTCTA CATCACAACC AAACAAGATG 
AATCCAATGC AATCAGCTTC CAATTTAGAT TCTGGACAAG GAAATTCACT GAGCTCATTG 
CAGCAGGCAC CTGTAGGATC TGTACAACAG AATCCTGTGA GCAGTTCCCA ACCTACCAAT 
TTTAACACAT TGTCAACACA AAGTGGGGTG AGTATGCTGC AGTCGAATAT TCCTCTTCAG 
TTGAATTCCA ATATGATTCA ACAGCAGCAT CTAAAACAGC AGATGCTACA AACACAACAG 
CTTAAACAAC AGTTCCAACA GCGTCAGCCG CAGCAGCAAC AGCGTCAGAC GCAGCAGCAA 
CAGCATTTAA TGCAGAAGCA ACAGATGCTG CTGCAGCAGC AGCAGCAGCA GTTACACCAG 
CAAGCCAAGC AGCAGTTGCC TGCACAGATG CAGACACAAC AAATTCCACA GCCGCATCAG 
ATGAATGATG TAAATGAGAT GAAGTTGAGA CAGGGGATTG GAATCAAGCC AGCAGTCTTC 
CAACAACATC TCTCGACAGG TCAACGCACT GCTTTCCCTC ATCAACAAAT GAAACCAGGA 
TCCTCATTTC CTATTTCTTC ACCACAAATG CTCCAGCATG CTTCCCCCCA ATTACAACAA 
CATTCTTCTC CTCAGATTGA CCAGCAAAAT CTACTGCCTT CTCTAACTAA AACTGGAACA 
CCTTTGCAAT CTGCAAACTC GCCTTTTGTT GTCCCATCTC CTTCAACTCC TTTAGCTCCA 
TCCCCTGTGC CAGGAGATTC TGAGAAACCC ATTTCTGGTA TCTCCTCAAA TATTGTACAC 
CAACCAACTG TTGCACAAGC AACGGCTCCA TCCCTTGCAA TTGGCACTCC TGGGATATCT 
GCCTCGCCTT TGCTTGCAGA GTTTACCAGT CCTGATGGTG CTCATGGTGG TGCTTTGACA 
ACTGTTTCTG GCAAGTCGAA TGTTATGGAG CAACCACTTG AACGCATGAT TAAAGCGGTG 
AAATCTCTTT CTCCTAAAGC ACTTAGTGCA TCTGTCAGTG ACATTGGCTC TGTTGTCAGT 
ATGATTGACA GAATTGCTGG CTCGGCACCC GGTAATGGGT CTAGAGCTGC AGTTGGTGAG 
GATCTGGTAG CGATGACAAA ATGTCGCCTT CAGGCAAGAA ATTTCTTCAC CCAAGATGGG 
ATGACTGGAT CGAAGAAAAT GAGGCGCCAT ACAAGTGCCA TGCCCTTGAA TGTTGCATCA 
TCAGCTGGCA GTGTAAGCGA CAGTTTTAAG CAGTTTACTG GTCCAGAGAC ATCTGATTTG 
GAGTCGACTG TAACGTCAAG TGTCAAGAGG CCTAAAATTG AGCTCTTCTT GCAGGCTAAT 
CATGCCCTTT TGGAAGAGAT AAGGGAAATA AATCAGCGAC TTTTAGACAC AGTGGTTGAT 
ATTAGTGATG AAGATGTTGA CTCAACTGCA GCAGCAGCTG CTGCTGAAGG GGGTGGAGGA 
ACCTTTGTCA AATGCTCATT TATCGCTGTG GCTCTGAGTC CAAACTTGAA AGCACAGTAC 
ACTTCAGCGC AAATGTCAAC AATTCAGCCC CTTAGATTGC TTGTGCCTAC AAATTATCCG 
AATTGCTCAC CGATACTGTT AGACAAGTTT CCATTTGAAG TCAGTAAAGA GTACGAGGAT 
TTGTCAATAA AAGCTAAATT TAGGTTTAGC ATCTCTCTTC GAAGCCTTTC ACAGCCCATG 
TCGCTTGGAG ACATAGCCAG GACTTGGGAT GTCTGTGCCC ATGCTGTTAT TTCAGAGCAT 
GCACAACAAA GTGGCGGAGG CACCTTCAGC TCAAAATATG GTTCTTGGGA AAACTGCGTG 
AGTGCAGCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024449451.1
CDS	213..4124
Translation	MDANNWRPTAPGGEPVMDTGDWRTQLQPDARQRIVNKIMETLKRHLPFSGQEGLQELKKIAVRFEEKIYTAATSQSDYLRKISLKMLAMENKSQSTIPNSLPPNSTGSGNKPLDPGGSHSMQSQVHNQGPSLTIPLPANQSQERQQLLSQNMQTGMASSGVQSSSGLTSALPPISSLTQTVPNSVGQNPNMQSISGVSQNPVGNPMGQGVPSNMLANSQRQGRQLVVPQQQQQPQNPQQYFYQQQLQQQLLKQKLQQGNPQHSLVQSHVQQQQQQQQNLLQSNQLQSSQQSGVQTPSVMQPSIMQTAPLSCLQQNQPSSVQQSTQPMLQQHPQPVLRQQQQPQQTAGIHQQQRLLGQQNNLPNLQQQQLMVQQNNLSSMHQQQLGSQSNVSSLQQQQLLGAQSGNSSMQTNQHPVHMLQQSKVTLQQQAQQSAGTLLPNQGQQSQPQLPQQQLMSQIQSQPVQLQQQSNPLQHDLQQRFQASGSLLQQQNVTDQQKQLYQSQRALPETSPTSLDSTTQTGHVNVNDWQEEIYQKIKVMKEMYFPEINEIYQRIAAKLPQHDSHPQQPKSEQLDKLKALKTMLERLIMFLQVPKNNIKLNFKEKLGYYENQILNFLNTSRPRKPVPNLQQGQLPQLHMQPMQRPQSQVPQLQSHENQLNPQLQSMNLQGSVPTMQQNNVPSLPHNSLSSLSGVSTSQPNKMNPMQSASNLDSGQGNSLSSLQQAPVGSVQQNPVSSSQPTNFNTLSTQSGVSMLQSNIPLQLNSNMIQQQHLKQQMLQTQQLKQQFQQRQPQQQQRQTQQQQHLMQKQQMLLQQQQQQLHQQAKQQLPAQMQTQQIPQPHQMNDVNEMKLRQGIGIKPAVFQQHLSTGQRTAFPHQQMKPGSSFPISSPQMLQHASPQLQQHSSPQIDQQNLLPSLTKTGTPLQSANSPFVVPSPSTPLAPSPVPGDSEKPISGISSNIVHQPTVAQATAPSLAIGTPGISASPLLAEFTSPDGAHGGALTTVSGKSNVMEQPLERMIKAVKSLSPKALSASVSDIGSVVSMIDRIAGSAPGNGSRAAVGEDLVAMTKCRLQARNFFTQDGMTGSKKMRRHTSAMPLNVASSAGSVSDSFKQFTGPETSDLESTVTSSVKRPKIELFLQANHALLEEIREINQRLLDTVVDISDEDVDSTAAAAAAEGGGGTFVKCSFIAVALSPNLKAQYTSAQMSTIQPLRLLVPTNYPNCSPILLDKFPFEVSKEYEDLSIKAKFRFSISLRSLSQPMSLGDIARTWDVCAHAVISEHAQQSGGGTFSSKYGSWENCVSAA

Target ORF information:

RefSeq Version	XM_024593683.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa mediator of RNA polymerase II transcription subunit 15a (LOC7470541), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024593683.1

ORF Insert Sequence:

ATGGATGCCA ATAATTGGAG GCCTACTGCC CCCGGTGGAG AACCTGTCAT GGACACGGGT 
GATTGGAGAA CTCAATTGCA GCCGGACGCA CGCCAAAGAA TAGTCAACAA AATCATGGAG 
ACATTAAAAA GACATCTTCC ATTTTCTGGT CAAGAGGGAT TACAAGAACT CAAGAAAATA 
GCCGTACGGT TTGAGGAAAA GATTTATACT GCTGCAACAA GTCAGTCTGA TTATCTGCGT 
AAAATATCTC TGAAGATGCT TGCAATGGAG AACAAGTCTC AAAGTACCAT ACCCAATTCT 
TTGCCACCCA ACTCCACTGG CAGTGGTAAC AAACCCCTGG ATCCAGGAGG ATCTCACAGT 
ATGCAGTCTC AAGTTCACAA TCAAGGGCCA TCACTCACTA TCCCATTGCC TGCTAATCAA 
TCTCAAGAAC GCCAGCAATT GTTGTCACAG AACATGCAGA CTGGTATGGC ATCTAGTGGA 
GTTCAAAGTT CTTCTGGTTT AACGTCAGCA TTGCCTCCCA TCTCCAGTTT AACCCAGACT 
GTCCCCAACT CTGTTGGCCA GAATCCTAAC ATGCAGAGTA TCTCTGGTGT TTCACAGAAC 
CCAGTGGGGA ATCCAATGGG ACAAGGGGTG CCCTCCAATA TGCTTGCCAA TTCTCAGAGA 
CAAGGTAGGC AACTAGTTGT TCCACAGCAA CAACAGCAGC CCCAAAATCC TCAGCAGTAT 
TTTTACCAGC AGCAGTTACA ACAACAGCTT TTGAAGCAGA AGCTTCAACA GGGAAATCCC 
CAGCACTCAC TTGTGCAATC TCACGTCCAG CAGCAGCAAC AGCAGCAGCA AAACCTATTA 
CAGTCAAATC AGTTGCAATC TTCTCAGCAG TCTGGTGTGC AAACACCATC TGTTATGCAG 
CCTTCAATAA TGCAAACGGC TCCTCTCTCT TGTCTTCAAC AGAATCAGCC ATCTTCTGTT 
CAACAGTCAA CACAACCCAT GCTTCAGCAA CATCCACAAC CAGTCCTCAG GCAGCAGCAA 
CAACCTCAAC AGACTGCTGG TATTCATCAG CAGCAGAGGT TGCTAGGCCA GCAGAATAAC 
CTTCCAAATC TGCAGCAGCA GCAATTAATG GTTCAACAAA ATAACCTATC AAGCATGCAT 
CAGCAACAGC TGGGCTCTCA AAGTAATGTC TCCAGTTTAC AGCAGCAGCA GCTGCTTGGA 
GCTCAGTCTG GTAACTCAAG CATGCAAACT AATCAGCACC CTGTACACAT GCTACAACAG 
TCCAAGGTTA CGCTGCAGCA ACAAGCACAA CAAAGTGCAG GTACTTTGTT ACCTAATCAA 
GGGCAGCAGT CACAACCACA GCTGCCACAG CAGCAATTGA TGTCACAGAT CCAATCACAG 
CCAGTGCAGC TGCAACAGCA ATCAAATCCA TTACAACATG ATCTGCAACA AAGATTTCAA 
GCATCAGGTT CCTTGCTTCA ACAGCAGAAT GTGACTGATC AGCAAAAGCA GTTATATCAA 
TCTCAAAGAG CCCTTCCAGA AACGTCCCCA ACATCCCTAG ATTCTACAAC ACAGACTGGA 
CATGTGAATG TCAATGATTG GCAAGAGGAG ATCTACCAAA AGATCAAAGT CATGAAGGAG 
ATGTATTTCC CTGAAATAAA TGAAATATAC CAGAGAATTG CTGCCAAGTT ACCGCAGCAT 
GACTCTCATC CGCAACAACC AAAGTCAGAA CAGCTTGACA AGCTAAAGGC ACTTAAGACC 
ATGTTGGAGC GCCTGATAAT GTTCTTACAA GTTCCGAAAA ACAACATCAA GCTTAATTTC 
AAAGAGAAGT TGGGTTACTA TGAGAATCAG ATTTTAAATT TTCTAAACAC AAGCAGACCC 
AGAAAGCCTG TTCCTAATCT TCAGCAAGGA CAGCTTCCCC AGCTTCATAT GCAACCTATG 
CAGCGACCCC AATCTCAAGT TCCTCAATTG CAGTCCCATG AAAATCAACT CAACCCCCAG 
TTGCAATCAA TGAATTTGCA AGGTTCTGTA CCAACAATGC AGCAGAACAA TGTGCCAAGC 
TTGCCGCATA ATTCTCTTTC TTCTTTATCT GGGGTTTCTA CATCACAACC AAACAAGATG 
AATCCAATGC AATCAGCTTC CAATTTAGAT TCTGGACAAG GAAATTCACT GAGCTCATTG 
CAGCAGGCAC CTGTAGGATC TGTACAACAG AATCCTGTGA GCAGTTCCCA ACCTACCAAT 
TTTAACACAT TGTCAACACA AAGTGGGGTG AGTATGCTGC AGTCGAATAT TCCTCTTCAG 
TTGAATTCCA ATATGATTCA ACAGCAGCAT CTAAAACAGC AGATGCTACA AACACAACAG 
CTTAAACAAC AGTTCCAACA GCGTCAGCCG CAGCAGCAAC AGCGTCAGAC GCAGCAGCAA 
CAGCATTTAA TGCAGAAGCA ACAGATGCTG CTGCAGCAGC AGCAGCAGCA GTTACACCAG 
CAAGCCAAGC AGCAGTTGCC TGCACAGATG CAGACACAAC AAATTCCACA GCCGCATCAG 
ATGAATGATG TAAATGAGAT GAAGTTGAGA CAGGGGATTG GAATCAAGCC AGCAGTCTTC 
CAACAACATC TCTCGACAGG TCAACGCACT GCTTTCCCTC ATCAACAAAT GAAACCAGGA 
TCCTCATTTC CTATTTCTTC ACCACAAATG CTCCAGCATG CTTCCCCCCA ATTACAACAA 
CATTCTTCTC CTCAGATTGA CCAGCAAAAT CTACTGCCTT CTCTAACTAA AACTGGAACA 
CCTTTGCAAT CTGCAAACTC GCCTTTTGTT GTCCCATCTC CTTCAACTCC TTTAGCTCCA 
TCCCCTGTGC CAGGAGATTC TGAGAAACCC ATTTCTGGTA TCTCCTCAAA TATTGTACAC 
CAACCAACTG TTGCACAAGC AACGGCTCCA TCCCTTGCAA TTGGCACTCC TGGGATATCT 
GCCTCGCCTT TGCTTGCAGA GTTTACCAGT CCTGATGGTG CTCATGGTGG TGCTTTGACA 
ACTGTTTCTG GCAAGTCGAA TGTTATGGAG CAACCACTTG AACGCATGAT TAAAGCGGTG 
AAATCTCTTT CTCCTAAAGC ACTTAGTGCA TCTGTCAGTG ACATTGGCTC TGTTGTCAGT 
ATGATTGACA GAATTGCTGG CTCGGCACCC GGTAATGGGT CTAGAGCTGC AGTTGGTGAG 
GATCTGGTAG CGATGACAAA ATGTCGCCTT CAGGCAAGAA ATTTCTTCAC CCAAGATGGG 
ATGACTGGAT CGAAGAAAAT GAGGCGCCAT ACAAGTGCCA TGCCCTTGAA TGTTGCATCA 
TCAGCTGGCA GTGTAAGCGA CAGTTTTAAG CAGTTTACTG GTCCAGAGAC ATCTGATTTG 
GAGTCGACTG TAACGTCAAG TGTCAAGAGG CCTAAAATTG AGCTCTTCTT GCAGGCTAAT 
CATGCCCTTT TGGAAGAGAT AAGGGAAATA AATCAGCGAC TTTTAGACAC AGTGGTTGAT 
ATTAGTGATG AAGATGTTGA CTCAACTGCA GCAGCAGCTG CTGCTGAAGG GGGTGGAGGA 
ACCTTTGTCA AATGCTCATT TATCGCTGTG GCTCTGAGTC CAAACTTGAA AGCACAGTAC 
ACTTCAGCGC AAATGTCAAC AATTCAGCCC CTTAGATTGC TTGTGCCTAC AAATTATCCG 
AATTGCTCAC CGATACTGTT AGACAAGTTT CCATTTGAAG TCAGTAAAGA GTACGAGGAT 
TTGTCAATAA AAGCTAAATT TAGGTTTAGC ATCTCTCTTC GAAGCCTTTC ACAGCCCATG 
TCGCTTGGAG ACATAGCCAG GACTTGGGAT GTCTGTGCCC ATGCTGTTAT TTCAGAGCAT 
GCACAACAAA GTGGCGGAGG CACCTTCAGC TCAAAATATG GTTCTTGGGA AAACTGCGTG 
AGTGCAGCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POPTR_0001s21200g ( XM_024593682.1 ) cDNA ORF clone, Populus trichocarpa(Populus balsamifera subsp. trichocarpa) -> XP_024449450.1 Populus trichocarpa mediator of RNA polymerase II transcription subunit 15a (LOC7470541), transcript variant X1, mRNA.

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CloneID	OPa423153
Clone ID Related Accession (Same CDS sequence)	XM_024593683.1 , XM_024593682.1 , XM_024593685.1
Accession Version	XM_024593682.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3912bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-08
Organism	Populus trichocarpa(black cottonwood)
Product	mediator of RNA polymerase II transcription subunit 15a isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037285.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCCA ATAATTGGAG GCCTACTGCC CCCGGTGGAG AACCTGTCAT GGACACGGGT 
GATTGGAGAA CTCAATTGCA GCCGGACGCA CGCCAAAGAA TAGTCAACAA AATCATGGAG 
ACATTAAAAA GACATCTTCC ATTTTCTGGT CAAGAGGGAT TACAAGAACT CAAGAAAATA 
GCCGTACGGT TTGAGGAAAA GATTTATACT GCTGCAACAA GTCAGTCTGA TTATCTGCGT 
AAAATATCTC TGAAGATGCT TGCAATGGAG AACAAGTCTC AAAGTACCAT ACCCAATTCT 
TTGCCACCCA ACTCCACTGG CAGTGGTAAC AAACCCCTGG ATCCAGGAGG ATCTCACAGT 
ATGCAGTCTC AAGTTCACAA TCAAGGGCCA TCACTCACTA TCCCATTGCC TGCTAATCAA 
TCTCAAGAAC GCCAGCAATT GTTGTCACAG AACATGCAGA CTGGTATGGC ATCTAGTGGA 
GTTCAAAGTT CTTCTGGTTT AACGTCAGCA TTGCCTCCCA TCTCCAGTTT AACCCAGACT 
GTCCCCAACT CTGTTGGCCA GAATCCTAAC ATGCAGAGTA TCTCTGGTGT TTCACAGAAC 
CCAGTGGGGA ATCCAATGGG ACAAGGGGTG CCCTCCAATA TGCTTGCCAA TTCTCAGAGA 
CAAGGTAGGC AACTAGTTGT TCCACAGCAA CAACAGCAGC CCCAAAATCC TCAGCAGTAT 
TTTTACCAGC AGCAGTTACA ACAACAGCTT TTGAAGCAGA AGCTTCAACA GGGAAATCCC 
CAGCACTCAC TTGTGCAATC TCACGTCCAG CAGCAGCAAC AGCAGCAGCA AAACCTATTA 
CAGTCAAATC AGTTGCAATC TTCTCAGCAG TCTGGTGTGC AAACACCATC TGTTATGCAG 
CCTTCAATAA TGCAAACGGC TCCTCTCTCT TGTCTTCAAC AGAATCAGCC ATCTTCTGTT 
CAACAGTCAA CACAACCCAT GCTTCAGCAA CATCCACAAC CAGTCCTCAG GCAGCAGCAA 
CAACCTCAAC AGACTGCTGG TATTCATCAG CAGCAGAGGT TGCTAGGCCA GCAGAATAAC 
CTTCCAAATC TGCAGCAGCA GCAATTAATG GTTCAACAAA ATAACCTATC AAGCATGCAT 
CAGCAACAGC TGGGCTCTCA AAGTAATGTC TCCAGTTTAC AGCAGCAGCA GCTGCTTGGA 
GCTCAGTCTG GTAACTCAAG CATGCAAACT AATCAGCACC CTGTACACAT GCTACAACAG 
TCCAAGGTTA CGCTGCAGCA ACAAGCACAA CAAAGTGCAG GTACTTTGTT ACCTAATCAA 
GGGCAGCAGT CACAACCACA GCTGCCACAG CAGCAATTGA TGTCACAGAT CCAATCACAG 
CCAGTGCAGC TGCAACAGCA ATCAAATCCA TTACAACATG ATCTGCAACA AAGATTTCAA 
GCATCAGGTT CCTTGCTTCA ACAGCAGAAT GTGACTGATC AGCAAAAGCA GTTATATCAA 
TCTCAAAGAG CCCTTCCAGA AACGTCCCCA ACATCCCTAG ATTCTACAAC ACAGACTGGA 
CATGTGAATG TCAATGATTG GCAAGAGGAG ATCTACCAAA AGATCAAAGT CATGAAGGAG 
ATGTATTTCC CTGAAATAAA TGAAATATAC CAGAGAATTG CTGCCAAGTT ACCGCAGCAT 
GACTCTCATC CGCAACAACC AAAGTCAGAA CAGCTTGACA AGCTAAAGGC ACTTAAGACC 
ATGTTGGAGC GCCTGATAAT GTTCTTACAA GTTCCGAAAA ACAACATCAA GCTTAATTTC 
AAAGAGAAGT TGGGTTACTA TGAGAATCAG ATTTTAAATT TTCTAAACAC AAGCAGACCC 
AGAAAGCCTG TTCCTAATCT TCAGCAAGGA CAGCTTCCCC AGCTTCATAT GCAACCTATG 
CAGCGACCCC AATCTCAAGT TCCTCAATTG CAGTCCCATG AAAATCAACT CAACCCCCAG 
TTGCAATCAA TGAATTTGCA AGGTTCTGTA CCAACAATGC AGCAGAACAA TGTGCCAAGC 
TTGCCGCATA ATTCTCTTTC TTCTTTATCT GGGGTTTCTA CATCACAACC AAACAAGATG 
AATCCAATGC AATCAGCTTC CAATTTAGAT TCTGGACAAG GAAATTCACT GAGCTCATTG 
CAGCAGGCAC CTGTAGGATC TGTACAACAG AATCCTGTGA GCAGTTCCCA ACCTACCAAT 
TTTAACACAT TGTCAACACA AAGTGGGGTG AGTATGCTGC AGTCGAATAT TCCTCTTCAG 
TTGAATTCCA ATATGATTCA ACAGCAGCAT CTAAAACAGC AGATGCTACA AACACAACAG 
CTTAAACAAC AGTTCCAACA GCGTCAGCCG CAGCAGCAAC AGCGTCAGAC GCAGCAGCAA 
CAGCATTTAA TGCAGAAGCA ACAGATGCTG CTGCAGCAGC AGCAGCAGCA GTTACACCAG 
CAAGCCAAGC AGCAGTTGCC TGCACAGATG CAGACACAAC AAATTCCACA GCCGCATCAG 
ATGAATGATG TAAATGAGAT GAAGTTGAGA CAGGGGATTG GAATCAAGCC AGCAGTCTTC 
CAACAACATC TCTCGACAGG TCAACGCACT GCTTTCCCTC ATCAACAAAT GAAACCAGGA 
TCCTCATTTC CTATTTCTTC ACCACAAATG CTCCAGCATG CTTCCCCCCA ATTACAACAA 
CATTCTTCTC CTCAGATTGA CCAGCAAAAT CTACTGCCTT CTCTAACTAA AACTGGAACA 
CCTTTGCAAT CTGCAAACTC GCCTTTTGTT GTCCCATCTC CTTCAACTCC TTTAGCTCCA 
TCCCCTGTGC CAGGAGATTC TGAGAAACCC ATTTCTGGTA TCTCCTCAAA TATTGTACAC 
CAACCAACTG TTGCACAAGC AACGGCTCCA TCCCTTGCAA TTGGCACTCC TGGGATATCT 
GCCTCGCCTT TGCTTGCAGA GTTTACCAGT CCTGATGGTG CTCATGGTGG TGCTTTGACA 
ACTGTTTCTG GCAAGTCGAA TGTTATGGAG CAACCACTTG AACGCATGAT TAAAGCGGTG 
AAATCTCTTT CTCCTAAAGC ACTTAGTGCA TCTGTCAGTG ACATTGGCTC TGTTGTCAGT 
ATGATTGACA GAATTGCTGG CTCGGCACCC GGTAATGGGT CTAGAGCTGC AGTTGGTGAG 
GATCTGGTAG CGATGACAAA ATGTCGCCTT CAGGCAAGAA ATTTCTTCAC CCAAGATGGG 
ATGACTGGAT CGAAGAAAAT GAGGCGCCAT ACAAGTGCCA TGCCCTTGAA TGTTGCATCA 
TCAGCTGGCA GTGTAAGCGA CAGTTTTAAG CAGTTTACTG GTCCAGAGAC ATCTGATTTG 
GAGTCGACTG TAACGTCAAG TGTCAAGAGG CCTAAAATTG AGCTCTTCTT GCAGGCTAAT 
CATGCCCTTT TGGAAGAGAT AAGGGAAATA AATCAGCGAC TTTTAGACAC AGTGGTTGAT 
ATTAGTGATG AAGATGTTGA CTCAACTGCA GCAGCAGCTG CTGCTGAAGG GGGTGGAGGA 
ACCTTTGTCA AATGCTCATT TATCGCTGTG GCTCTGAGTC CAAACTTGAA AGCACAGTAC 
ACTTCAGCGC AAATGTCAAC AATTCAGCCC CTTAGATTGC TTGTGCCTAC AAATTATCCG 
AATTGCTCAC CGATACTGTT AGACAAGTTT CCATTTGAAG TCAGTAAAGA GTACGAGGAT 
TTGTCAATAA AAGCTAAATT TAGGTTTAGC ATCTCTCTTC GAAGCCTTTC ACAGCCCATG 
TCGCTTGGAG ACATAGCCAG GACTTGGGAT GTCTGTGCCC ATGCTGTTAT TTCAGAGCAT 
GCACAACAAA GTGGCGGAGG CACCTTCAGC TCAAAATATG GTTCTTGGGA AAACTGCGTG 
AGTGCAGCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024449450.1
CDS	216..4127
Translation	MDANNWRPTAPGGEPVMDTGDWRTQLQPDARQRIVNKIMETLKRHLPFSGQEGLQELKKIAVRFEEKIYTAATSQSDYLRKISLKMLAMENKSQSTIPNSLPPNSTGSGNKPLDPGGSHSMQSQVHNQGPSLTIPLPANQSQERQQLLSQNMQTGMASSGVQSSSGLTSALPPISSLTQTVPNSVGQNPNMQSISGVSQNPVGNPMGQGVPSNMLANSQRQGRQLVVPQQQQQPQNPQQYFYQQQLQQQLLKQKLQQGNPQHSLVQSHVQQQQQQQQNLLQSNQLQSSQQSGVQTPSVMQPSIMQTAPLSCLQQNQPSSVQQSTQPMLQQHPQPVLRQQQQPQQTAGIHQQQRLLGQQNNLPNLQQQQLMVQQNNLSSMHQQQLGSQSNVSSLQQQQLLGAQSGNSSMQTNQHPVHMLQQSKVTLQQQAQQSAGTLLPNQGQQSQPQLPQQQLMSQIQSQPVQLQQQSNPLQHDLQQRFQASGSLLQQQNVTDQQKQLYQSQRALPETSPTSLDSTTQTGHVNVNDWQEEIYQKIKVMKEMYFPEINEIYQRIAAKLPQHDSHPQQPKSEQLDKLKALKTMLERLIMFLQVPKNNIKLNFKEKLGYYENQILNFLNTSRPRKPVPNLQQGQLPQLHMQPMQRPQSQVPQLQSHENQLNPQLQSMNLQGSVPTMQQNNVPSLPHNSLSSLSGVSTSQPNKMNPMQSASNLDSGQGNSLSSLQQAPVGSVQQNPVSSSQPTNFNTLSTQSGVSMLQSNIPLQLNSNMIQQQHLKQQMLQTQQLKQQFQQRQPQQQQRQTQQQQHLMQKQQMLLQQQQQQLHQQAKQQLPAQMQTQQIPQPHQMNDVNEMKLRQGIGIKPAVFQQHLSTGQRTAFPHQQMKPGSSFPISSPQMLQHASPQLQQHSSPQIDQQNLLPSLTKTGTPLQSANSPFVVPSPSTPLAPSPVPGDSEKPISGISSNIVHQPTVAQATAPSLAIGTPGISASPLLAEFTSPDGAHGGALTTVSGKSNVMEQPLERMIKAVKSLSPKALSASVSDIGSVVSMIDRIAGSAPGNGSRAAVGEDLVAMTKCRLQARNFFTQDGMTGSKKMRRHTSAMPLNVASSAGSVSDSFKQFTGPETSDLESTVTSSVKRPKIELFLQANHALLEEIREINQRLLDTVVDISDEDVDSTAAAAAAEGGGGTFVKCSFIAVALSPNLKAQYTSAQMSTIQPLRLLVPTNYPNCSPILLDKFPFEVSKEYEDLSIKAKFRFSISLRSLSQPMSLGDIARTWDVCAHAVISEHAQQSGGGTFSSKYGSWENCVSAA

Target ORF information:

RefSeq Version	XM_024593682.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa mediator of RNA polymerase II transcription subunit 15a (LOC7470541), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024593682.1

ORF Insert Sequence:

ATGGATGCCA ATAATTGGAG GCCTACTGCC CCCGGTGGAG AACCTGTCAT GGACACGGGT 
GATTGGAGAA CTCAATTGCA GCCGGACGCA CGCCAAAGAA TAGTCAACAA AATCATGGAG 
ACATTAAAAA GACATCTTCC ATTTTCTGGT CAAGAGGGAT TACAAGAACT CAAGAAAATA 
GCCGTACGGT TTGAGGAAAA GATTTATACT GCTGCAACAA GTCAGTCTGA TTATCTGCGT 
AAAATATCTC TGAAGATGCT TGCAATGGAG AACAAGTCTC AAAGTACCAT ACCCAATTCT 
TTGCCACCCA ACTCCACTGG CAGTGGTAAC AAACCCCTGG ATCCAGGAGG ATCTCACAGT 
ATGCAGTCTC AAGTTCACAA TCAAGGGCCA TCACTCACTA TCCCATTGCC TGCTAATCAA 
TCTCAAGAAC GCCAGCAATT GTTGTCACAG AACATGCAGA CTGGTATGGC ATCTAGTGGA 
GTTCAAAGTT CTTCTGGTTT AACGTCAGCA TTGCCTCCCA TCTCCAGTTT AACCCAGACT 
GTCCCCAACT CTGTTGGCCA GAATCCTAAC ATGCAGAGTA TCTCTGGTGT TTCACAGAAC 
CCAGTGGGGA ATCCAATGGG ACAAGGGGTG CCCTCCAATA TGCTTGCCAA TTCTCAGAGA 
CAAGGTAGGC AACTAGTTGT TCCACAGCAA CAACAGCAGC CCCAAAATCC TCAGCAGTAT 
TTTTACCAGC AGCAGTTACA ACAACAGCTT TTGAAGCAGA AGCTTCAACA GGGAAATCCC 
CAGCACTCAC TTGTGCAATC TCACGTCCAG CAGCAGCAAC AGCAGCAGCA AAACCTATTA 
CAGTCAAATC AGTTGCAATC TTCTCAGCAG TCTGGTGTGC AAACACCATC TGTTATGCAG 
CCTTCAATAA TGCAAACGGC TCCTCTCTCT TGTCTTCAAC AGAATCAGCC ATCTTCTGTT 
CAACAGTCAA CACAACCCAT GCTTCAGCAA CATCCACAAC CAGTCCTCAG GCAGCAGCAA 
CAACCTCAAC AGACTGCTGG TATTCATCAG CAGCAGAGGT TGCTAGGCCA GCAGAATAAC 
CTTCCAAATC TGCAGCAGCA GCAATTAATG GTTCAACAAA ATAACCTATC AAGCATGCAT 
CAGCAACAGC TGGGCTCTCA AAGTAATGTC TCCAGTTTAC AGCAGCAGCA GCTGCTTGGA 
GCTCAGTCTG GTAACTCAAG CATGCAAACT AATCAGCACC CTGTACACAT GCTACAACAG 
TCCAAGGTTA CGCTGCAGCA ACAAGCACAA CAAAGTGCAG GTACTTTGTT ACCTAATCAA 
GGGCAGCAGT CACAACCACA GCTGCCACAG CAGCAATTGA TGTCACAGAT CCAATCACAG 
CCAGTGCAGC TGCAACAGCA ATCAAATCCA TTACAACATG ATCTGCAACA AAGATTTCAA 
GCATCAGGTT CCTTGCTTCA ACAGCAGAAT GTGACTGATC AGCAAAAGCA GTTATATCAA 
TCTCAAAGAG CCCTTCCAGA AACGTCCCCA ACATCCCTAG ATTCTACAAC ACAGACTGGA 
CATGTGAATG TCAATGATTG GCAAGAGGAG ATCTACCAAA AGATCAAAGT CATGAAGGAG 
ATGTATTTCC CTGAAATAAA TGAAATATAC CAGAGAATTG CTGCCAAGTT ACCGCAGCAT 
GACTCTCATC CGCAACAACC AAAGTCAGAA CAGCTTGACA AGCTAAAGGC ACTTAAGACC 
ATGTTGGAGC GCCTGATAAT GTTCTTACAA GTTCCGAAAA ACAACATCAA GCTTAATTTC 
AAAGAGAAGT TGGGTTACTA TGAGAATCAG ATTTTAAATT TTCTAAACAC AAGCAGACCC 
AGAAAGCCTG TTCCTAATCT TCAGCAAGGA CAGCTTCCCC AGCTTCATAT GCAACCTATG 
CAGCGACCCC AATCTCAAGT TCCTCAATTG CAGTCCCATG AAAATCAACT CAACCCCCAG 
TTGCAATCAA TGAATTTGCA AGGTTCTGTA CCAACAATGC AGCAGAACAA TGTGCCAAGC 
TTGCCGCATA ATTCTCTTTC TTCTTTATCT GGGGTTTCTA CATCACAACC AAACAAGATG 
AATCCAATGC AATCAGCTTC CAATTTAGAT TCTGGACAAG GAAATTCACT GAGCTCATTG 
CAGCAGGCAC CTGTAGGATC TGTACAACAG AATCCTGTGA GCAGTTCCCA ACCTACCAAT 
TTTAACACAT TGTCAACACA AAGTGGGGTG AGTATGCTGC AGTCGAATAT TCCTCTTCAG 
TTGAATTCCA ATATGATTCA ACAGCAGCAT CTAAAACAGC AGATGCTACA AACACAACAG 
CTTAAACAAC AGTTCCAACA GCGTCAGCCG CAGCAGCAAC AGCGTCAGAC GCAGCAGCAA 
CAGCATTTAA TGCAGAAGCA ACAGATGCTG CTGCAGCAGC AGCAGCAGCA GTTACACCAG 
CAAGCCAAGC AGCAGTTGCC TGCACAGATG CAGACACAAC AAATTCCACA GCCGCATCAG 
ATGAATGATG TAAATGAGAT GAAGTTGAGA CAGGGGATTG GAATCAAGCC AGCAGTCTTC 
CAACAACATC TCTCGACAGG TCAACGCACT GCTTTCCCTC ATCAACAAAT GAAACCAGGA 
TCCTCATTTC CTATTTCTTC ACCACAAATG CTCCAGCATG CTTCCCCCCA ATTACAACAA 
CATTCTTCTC CTCAGATTGA CCAGCAAAAT CTACTGCCTT CTCTAACTAA AACTGGAACA 
CCTTTGCAAT CTGCAAACTC GCCTTTTGTT GTCCCATCTC CTTCAACTCC TTTAGCTCCA 
TCCCCTGTGC CAGGAGATTC TGAGAAACCC ATTTCTGGTA TCTCCTCAAA TATTGTACAC 
CAACCAACTG TTGCACAAGC AACGGCTCCA TCCCTTGCAA TTGGCACTCC TGGGATATCT 
GCCTCGCCTT TGCTTGCAGA GTTTACCAGT CCTGATGGTG CTCATGGTGG TGCTTTGACA 
ACTGTTTCTG GCAAGTCGAA TGTTATGGAG CAACCACTTG AACGCATGAT TAAAGCGGTG 
AAATCTCTTT CTCCTAAAGC ACTTAGTGCA TCTGTCAGTG ACATTGGCTC TGTTGTCAGT 
ATGATTGACA GAATTGCTGG CTCGGCACCC GGTAATGGGT CTAGAGCTGC AGTTGGTGAG 
GATCTGGTAG CGATGACAAA ATGTCGCCTT CAGGCAAGAA ATTTCTTCAC CCAAGATGGG 
ATGACTGGAT CGAAGAAAAT GAGGCGCCAT ACAAGTGCCA TGCCCTTGAA TGTTGCATCA 
TCAGCTGGCA GTGTAAGCGA CAGTTTTAAG CAGTTTACTG GTCCAGAGAC ATCTGATTTG 
GAGTCGACTG TAACGTCAAG TGTCAAGAGG CCTAAAATTG AGCTCTTCTT GCAGGCTAAT 
CATGCCCTTT TGGAAGAGAT AAGGGAAATA AATCAGCGAC TTTTAGACAC AGTGGTTGAT 
ATTAGTGATG AAGATGTTGA CTCAACTGCA GCAGCAGCTG CTGCTGAAGG GGGTGGAGGA 
ACCTTTGTCA AATGCTCATT TATCGCTGTG GCTCTGAGTC CAAACTTGAA AGCACAGTAC 
ACTTCAGCGC AAATGTCAAC AATTCAGCCC CTTAGATTGC TTGTGCCTAC AAATTATCCG 
AATTGCTCAC CGATACTGTT AGACAAGTTT CCATTTGAAG TCAGTAAAGA GTACGAGGAT 
TTGTCAATAA AAGCTAAATT TAGGTTTAGC ATCTCTCTTC GAAGCCTTTC ACAGCCCATG 
TCGCTTGGAG ACATAGCCAG GACTTGGGAT GTCTGTGCCC ATGCTGTTAT TTCAGAGCAT 
GCACAACAAA GTGGCGGAGG CACCTTCAGC TCAAAATATG GTTCTTGGGA AAACTGCGTG 
AGTGCAGCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POPTR_0001s21200g ( XM_024593685.1 ) cDNA ORF clone, Populus trichocarpa(Populus balsamifera subsp. trichocarpa) -> XP_024449453.1 Populus trichocarpa mediator of RNA polymerase II transcription subunit 15a (LOC7470541), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OPa423153
Clone ID Related Accession (Same CDS sequence)	XM_024593683.1 , XM_024593682.1 , XM_024593685.1
Accession Version	XM_024593685.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3912bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-08
Organism	Populus trichocarpa(black cottonwood)
Product	mediator of RNA polymerase II transcription subunit 15a isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037285.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCCA ATAATTGGAG GCCTACTGCC CCCGGTGGAG AACCTGTCAT GGACACGGGT 
GATTGGAGAA CTCAATTGCA GCCGGACGCA CGCCAAAGAA TAGTCAACAA AATCATGGAG 
ACATTAAAAA GACATCTTCC ATTTTCTGGT CAAGAGGGAT TACAAGAACT CAAGAAAATA 
GCCGTACGGT TTGAGGAAAA GATTTATACT GCTGCAACAA GTCAGTCTGA TTATCTGCGT 
AAAATATCTC TGAAGATGCT TGCAATGGAG AACAAGTCTC AAAGTACCAT ACCCAATTCT 
TTGCCACCCA ACTCCACTGG CAGTGGTAAC AAACCCCTGG ATCCAGGAGG ATCTCACAGT 
ATGCAGTCTC AAGTTCACAA TCAAGGGCCA TCACTCACTA TCCCATTGCC TGCTAATCAA 
TCTCAAGAAC GCCAGCAATT GTTGTCACAG AACATGCAGA CTGGTATGGC ATCTAGTGGA 
GTTCAAAGTT CTTCTGGTTT AACGTCAGCA TTGCCTCCCA TCTCCAGTTT AACCCAGACT 
GTCCCCAACT CTGTTGGCCA GAATCCTAAC ATGCAGAGTA TCTCTGGTGT TTCACAGAAC 
CCAGTGGGGA ATCCAATGGG ACAAGGGGTG CCCTCCAATA TGCTTGCCAA TTCTCAGAGA 
CAAGGTAGGC AACTAGTTGT TCCACAGCAA CAACAGCAGC CCCAAAATCC TCAGCAGTAT 
TTTTACCAGC AGCAGTTACA ACAACAGCTT TTGAAGCAGA AGCTTCAACA GGGAAATCCC 
CAGCACTCAC TTGTGCAATC TCACGTCCAG CAGCAGCAAC AGCAGCAGCA AAACCTATTA 
CAGTCAAATC AGTTGCAATC TTCTCAGCAG TCTGGTGTGC AAACACCATC TGTTATGCAG 
CCTTCAATAA TGCAAACGGC TCCTCTCTCT TGTCTTCAAC AGAATCAGCC ATCTTCTGTT 
CAACAGTCAA CACAACCCAT GCTTCAGCAA CATCCACAAC CAGTCCTCAG GCAGCAGCAA 
CAACCTCAAC AGACTGCTGG TATTCATCAG CAGCAGAGGT TGCTAGGCCA GCAGAATAAC 
CTTCCAAATC TGCAGCAGCA GCAATTAATG GTTCAACAAA ATAACCTATC AAGCATGCAT 
CAGCAACAGC TGGGCTCTCA AAGTAATGTC TCCAGTTTAC AGCAGCAGCA GCTGCTTGGA 
GCTCAGTCTG GTAACTCAAG CATGCAAACT AATCAGCACC CTGTACACAT GCTACAACAG 
TCCAAGGTTA CGCTGCAGCA ACAAGCACAA CAAAGTGCAG GTACTTTGTT ACCTAATCAA 
GGGCAGCAGT CACAACCACA GCTGCCACAG CAGCAATTGA TGTCACAGAT CCAATCACAG 
CCAGTGCAGC TGCAACAGCA ATCAAATCCA TTACAACATG ATCTGCAACA AAGATTTCAA 
GCATCAGGTT CCTTGCTTCA ACAGCAGAAT GTGACTGATC AGCAAAAGCA GTTATATCAA 
TCTCAAAGAG CCCTTCCAGA AACGTCCCCA ACATCCCTAG ATTCTACAAC ACAGACTGGA 
CATGTGAATG TCAATGATTG GCAAGAGGAG ATCTACCAAA AGATCAAAGT CATGAAGGAG 
ATGTATTTCC CTGAAATAAA TGAAATATAC CAGAGAATTG CTGCCAAGTT ACCGCAGCAT 
GACTCTCATC CGCAACAACC AAAGTCAGAA CAGCTTGACA AGCTAAAGGC ACTTAAGACC 
ATGTTGGAGC GCCTGATAAT GTTCTTACAA GTTCCGAAAA ACAACATCAA GCTTAATTTC 
AAAGAGAAGT TGGGTTACTA TGAGAATCAG ATTTTAAATT TTCTAAACAC AAGCAGACCC 
AGAAAGCCTG TTCCTAATCT TCAGCAAGGA CAGCTTCCCC AGCTTCATAT GCAACCTATG 
CAGCGACCCC AATCTCAAGT TCCTCAATTG CAGTCCCATG AAAATCAACT CAACCCCCAG 
TTGCAATCAA TGAATTTGCA AGGTTCTGTA CCAACAATGC AGCAGAACAA TGTGCCAAGC 
TTGCCGCATA ATTCTCTTTC TTCTTTATCT GGGGTTTCTA CATCACAACC AAACAAGATG 
AATCCAATGC AATCAGCTTC CAATTTAGAT TCTGGACAAG GAAATTCACT GAGCTCATTG 
CAGCAGGCAC CTGTAGGATC TGTACAACAG AATCCTGTGA GCAGTTCCCA ACCTACCAAT 
TTTAACACAT TGTCAACACA AAGTGGGGTG AGTATGCTGC AGTCGAATAT TCCTCTTCAG 
TTGAATTCCA ATATGATTCA ACAGCAGCAT CTAAAACAGC AGATGCTACA AACACAACAG 
CTTAAACAAC AGTTCCAACA GCGTCAGCCG CAGCAGCAAC AGCGTCAGAC GCAGCAGCAA 
CAGCATTTAA TGCAGAAGCA ACAGATGCTG CTGCAGCAGC AGCAGCAGCA GTTACACCAG 
CAAGCCAAGC AGCAGTTGCC TGCACAGATG CAGACACAAC AAATTCCACA GCCGCATCAG 
ATGAATGATG TAAATGAGAT GAAGTTGAGA CAGGGGATTG GAATCAAGCC AGCAGTCTTC 
CAACAACATC TCTCGACAGG TCAACGCACT GCTTTCCCTC ATCAACAAAT GAAACCAGGA 
TCCTCATTTC CTATTTCTTC ACCACAAATG CTCCAGCATG CTTCCCCCCA ATTACAACAA 
CATTCTTCTC CTCAGATTGA CCAGCAAAAT CTACTGCCTT CTCTAACTAA AACTGGAACA 
CCTTTGCAAT CTGCAAACTC GCCTTTTGTT GTCCCATCTC CTTCAACTCC TTTAGCTCCA 
TCCCCTGTGC CAGGAGATTC TGAGAAACCC ATTTCTGGTA TCTCCTCAAA TATTGTACAC 
CAACCAACTG TTGCACAAGC AACGGCTCCA TCCCTTGCAA TTGGCACTCC TGGGATATCT 
GCCTCGCCTT TGCTTGCAGA GTTTACCAGT CCTGATGGTG CTCATGGTGG TGCTTTGACA 
ACTGTTTCTG GCAAGTCGAA TGTTATGGAG CAACCACTTG AACGCATGAT TAAAGCGGTG 
AAATCTCTTT CTCCTAAAGC ACTTAGTGCA TCTGTCAGTG ACATTGGCTC TGTTGTCAGT 
ATGATTGACA GAATTGCTGG CTCGGCACCC GGTAATGGGT CTAGAGCTGC AGTTGGTGAG 
GATCTGGTAG CGATGACAAA ATGTCGCCTT CAGGCAAGAA ATTTCTTCAC CCAAGATGGG 
ATGACTGGAT CGAAGAAAAT GAGGCGCCAT ACAAGTGCCA TGCCCTTGAA TGTTGCATCA 
TCAGCTGGCA GTGTAAGCGA CAGTTTTAAG CAGTTTACTG GTCCAGAGAC ATCTGATTTG 
GAGTCGACTG TAACGTCAAG TGTCAAGAGG CCTAAAATTG AGCTCTTCTT GCAGGCTAAT 
CATGCCCTTT TGGAAGAGAT AAGGGAAATA AATCAGCGAC TTTTAGACAC AGTGGTTGAT 
ATTAGTGATG AAGATGTTGA CTCAACTGCA GCAGCAGCTG CTGCTGAAGG GGGTGGAGGA 
ACCTTTGTCA AATGCTCATT TATCGCTGTG GCTCTGAGTC CAAACTTGAA AGCACAGTAC 
ACTTCAGCGC AAATGTCAAC AATTCAGCCC CTTAGATTGC TTGTGCCTAC AAATTATCCG 
AATTGCTCAC CGATACTGTT AGACAAGTTT CCATTTGAAG TCAGTAAAGA GTACGAGGAT 
TTGTCAATAA AAGCTAAATT TAGGTTTAGC ATCTCTCTTC GAAGCCTTTC ACAGCCCATG 
TCGCTTGGAG ACATAGCCAG GACTTGGGAT GTCTGTGCCC ATGCTGTTAT TTCAGAGCAT 
GCACAACAAA GTGGCGGAGG CACCTTCAGC TCAAAATATG GTTCTTGGGA AAACTGCGTG 
AGTGCAGCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024449453.1
CDS	259..4170
Translation	MDANNWRPTAPGGEPVMDTGDWRTQLQPDARQRIVNKIMETLKRHLPFSGQEGLQELKKIAVRFEEKIYTAATSQSDYLRKISLKMLAMENKSQSTIPNSLPPNSTGSGNKPLDPGGSHSMQSQVHNQGPSLTIPLPANQSQERQQLLSQNMQTGMASSGVQSSSGLTSALPPISSLTQTVPNSVGQNPNMQSISGVSQNPVGNPMGQGVPSNMLANSQRQGRQLVVPQQQQQPQNPQQYFYQQQLQQQLLKQKLQQGNPQHSLVQSHVQQQQQQQQNLLQSNQLQSSQQSGVQTPSVMQPSIMQTAPLSCLQQNQPSSVQQSTQPMLQQHPQPVLRQQQQPQQTAGIHQQQRLLGQQNNLPNLQQQQLMVQQNNLSSMHQQQLGSQSNVSSLQQQQLLGAQSGNSSMQTNQHPVHMLQQSKVTLQQQAQQSAGTLLPNQGQQSQPQLPQQQLMSQIQSQPVQLQQQSNPLQHDLQQRFQASGSLLQQQNVTDQQKQLYQSQRALPETSPTSLDSTTQTGHVNVNDWQEEIYQKIKVMKEMYFPEINEIYQRIAAKLPQHDSHPQQPKSEQLDKLKALKTMLERLIMFLQVPKNNIKLNFKEKLGYYENQILNFLNTSRPRKPVPNLQQGQLPQLHMQPMQRPQSQVPQLQSHENQLNPQLQSMNLQGSVPTMQQNNVPSLPHNSLSSLSGVSTSQPNKMNPMQSASNLDSGQGNSLSSLQQAPVGSVQQNPVSSSQPTNFNTLSTQSGVSMLQSNIPLQLNSNMIQQQHLKQQMLQTQQLKQQFQQRQPQQQQRQTQQQQHLMQKQQMLLQQQQQQLHQQAKQQLPAQMQTQQIPQPHQMNDVNEMKLRQGIGIKPAVFQQHLSTGQRTAFPHQQMKPGSSFPISSPQMLQHASPQLQQHSSPQIDQQNLLPSLTKTGTPLQSANSPFVVPSPSTPLAPSPVPGDSEKPISGISSNIVHQPTVAQATAPSLAIGTPGISASPLLAEFTSPDGAHGGALTTVSGKSNVMEQPLERMIKAVKSLSPKALSASVSDIGSVVSMIDRIAGSAPGNGSRAAVGEDLVAMTKCRLQARNFFTQDGMTGSKKMRRHTSAMPLNVASSAGSVSDSFKQFTGPETSDLESTVTSSVKRPKIELFLQANHALLEEIREINQRLLDTVVDISDEDVDSTAAAAAAEGGGGTFVKCSFIAVALSPNLKAQYTSAQMSTIQPLRLLVPTNYPNCSPILLDKFPFEVSKEYEDLSIKAKFRFSISLRSLSQPMSLGDIARTWDVCAHAVISEHAQQSGGGTFSSKYGSWENCVSAA

Target ORF information:

RefSeq Version	XM_024593685.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa mediator of RNA polymerase II transcription subunit 15a (LOC7470541), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024593685.1

ORF Insert Sequence:

ATGGATGCCA ATAATTGGAG GCCTACTGCC CCCGGTGGAG AACCTGTCAT GGACACGGGT 
GATTGGAGAA CTCAATTGCA GCCGGACGCA CGCCAAAGAA TAGTCAACAA AATCATGGAG 
ACATTAAAAA GACATCTTCC ATTTTCTGGT CAAGAGGGAT TACAAGAACT CAAGAAAATA 
GCCGTACGGT TTGAGGAAAA GATTTATACT GCTGCAACAA GTCAGTCTGA TTATCTGCGT 
AAAATATCTC TGAAGATGCT TGCAATGGAG AACAAGTCTC AAAGTACCAT ACCCAATTCT 
TTGCCACCCA ACTCCACTGG CAGTGGTAAC AAACCCCTGG ATCCAGGAGG ATCTCACAGT 
ATGCAGTCTC AAGTTCACAA TCAAGGGCCA TCACTCACTA TCCCATTGCC TGCTAATCAA 
TCTCAAGAAC GCCAGCAATT GTTGTCACAG AACATGCAGA CTGGTATGGC ATCTAGTGGA 
GTTCAAAGTT CTTCTGGTTT AACGTCAGCA TTGCCTCCCA TCTCCAGTTT AACCCAGACT 
GTCCCCAACT CTGTTGGCCA GAATCCTAAC ATGCAGAGTA TCTCTGGTGT TTCACAGAAC 
CCAGTGGGGA ATCCAATGGG ACAAGGGGTG CCCTCCAATA TGCTTGCCAA TTCTCAGAGA 
CAAGGTAGGC AACTAGTTGT TCCACAGCAA CAACAGCAGC CCCAAAATCC TCAGCAGTAT 
TTTTACCAGC AGCAGTTACA ACAACAGCTT TTGAAGCAGA AGCTTCAACA GGGAAATCCC 
CAGCACTCAC TTGTGCAATC TCACGTCCAG CAGCAGCAAC AGCAGCAGCA AAACCTATTA 
CAGTCAAATC AGTTGCAATC TTCTCAGCAG TCTGGTGTGC AAACACCATC TGTTATGCAG 
CCTTCAATAA TGCAAACGGC TCCTCTCTCT TGTCTTCAAC AGAATCAGCC ATCTTCTGTT 
CAACAGTCAA CACAACCCAT GCTTCAGCAA CATCCACAAC CAGTCCTCAG GCAGCAGCAA 
CAACCTCAAC AGACTGCTGG TATTCATCAG CAGCAGAGGT TGCTAGGCCA GCAGAATAAC 
CTTCCAAATC TGCAGCAGCA GCAATTAATG GTTCAACAAA ATAACCTATC AAGCATGCAT 
CAGCAACAGC TGGGCTCTCA AAGTAATGTC TCCAGTTTAC AGCAGCAGCA GCTGCTTGGA 
GCTCAGTCTG GTAACTCAAG CATGCAAACT AATCAGCACC CTGTACACAT GCTACAACAG 
TCCAAGGTTA CGCTGCAGCA ACAAGCACAA CAAAGTGCAG GTACTTTGTT ACCTAATCAA 
GGGCAGCAGT CACAACCACA GCTGCCACAG CAGCAATTGA TGTCACAGAT CCAATCACAG 
CCAGTGCAGC TGCAACAGCA ATCAAATCCA TTACAACATG ATCTGCAACA AAGATTTCAA 
GCATCAGGTT CCTTGCTTCA ACAGCAGAAT GTGACTGATC AGCAAAAGCA GTTATATCAA 
TCTCAAAGAG CCCTTCCAGA AACGTCCCCA ACATCCCTAG ATTCTACAAC ACAGACTGGA 
CATGTGAATG TCAATGATTG GCAAGAGGAG ATCTACCAAA AGATCAAAGT CATGAAGGAG 
ATGTATTTCC CTGAAATAAA TGAAATATAC CAGAGAATTG CTGCCAAGTT ACCGCAGCAT 
GACTCTCATC CGCAACAACC AAAGTCAGAA CAGCTTGACA AGCTAAAGGC ACTTAAGACC 
ATGTTGGAGC GCCTGATAAT GTTCTTACAA GTTCCGAAAA ACAACATCAA GCTTAATTTC 
AAAGAGAAGT TGGGTTACTA TGAGAATCAG ATTTTAAATT TTCTAAACAC AAGCAGACCC 
AGAAAGCCTG TTCCTAATCT TCAGCAAGGA CAGCTTCCCC AGCTTCATAT GCAACCTATG 
CAGCGACCCC AATCTCAAGT TCCTCAATTG CAGTCCCATG AAAATCAACT CAACCCCCAG 
TTGCAATCAA TGAATTTGCA AGGTTCTGTA CCAACAATGC AGCAGAACAA TGTGCCAAGC 
TTGCCGCATA ATTCTCTTTC TTCTTTATCT GGGGTTTCTA CATCACAACC AAACAAGATG 
AATCCAATGC AATCAGCTTC CAATTTAGAT TCTGGACAAG GAAATTCACT GAGCTCATTG 
CAGCAGGCAC CTGTAGGATC TGTACAACAG AATCCTGTGA GCAGTTCCCA ACCTACCAAT 
TTTAACACAT TGTCAACACA AAGTGGGGTG AGTATGCTGC AGTCGAATAT TCCTCTTCAG 
TTGAATTCCA ATATGATTCA ACAGCAGCAT CTAAAACAGC AGATGCTACA AACACAACAG 
CTTAAACAAC AGTTCCAACA GCGTCAGCCG CAGCAGCAAC AGCGTCAGAC GCAGCAGCAA 
CAGCATTTAA TGCAGAAGCA ACAGATGCTG CTGCAGCAGC AGCAGCAGCA GTTACACCAG 
CAAGCCAAGC AGCAGTTGCC TGCACAGATG CAGACACAAC AAATTCCACA GCCGCATCAG 
ATGAATGATG TAAATGAGAT GAAGTTGAGA CAGGGGATTG GAATCAAGCC AGCAGTCTTC 
CAACAACATC TCTCGACAGG TCAACGCACT GCTTTCCCTC ATCAACAAAT GAAACCAGGA 
TCCTCATTTC CTATTTCTTC ACCACAAATG CTCCAGCATG CTTCCCCCCA ATTACAACAA 
CATTCTTCTC CTCAGATTGA CCAGCAAAAT CTACTGCCTT CTCTAACTAA AACTGGAACA 
CCTTTGCAAT CTGCAAACTC GCCTTTTGTT GTCCCATCTC CTTCAACTCC TTTAGCTCCA 
TCCCCTGTGC CAGGAGATTC TGAGAAACCC ATTTCTGGTA TCTCCTCAAA TATTGTACAC 
CAACCAACTG TTGCACAAGC AACGGCTCCA TCCCTTGCAA TTGGCACTCC TGGGATATCT 
GCCTCGCCTT TGCTTGCAGA GTTTACCAGT CCTGATGGTG CTCATGGTGG TGCTTTGACA 
ACTGTTTCTG GCAAGTCGAA TGTTATGGAG CAACCACTTG AACGCATGAT TAAAGCGGTG 
AAATCTCTTT CTCCTAAAGC ACTTAGTGCA TCTGTCAGTG ACATTGGCTC TGTTGTCAGT 
ATGATTGACA GAATTGCTGG CTCGGCACCC GGTAATGGGT CTAGAGCTGC AGTTGGTGAG 
GATCTGGTAG CGATGACAAA ATGTCGCCTT CAGGCAAGAA ATTTCTTCAC CCAAGATGGG 
ATGACTGGAT CGAAGAAAAT GAGGCGCCAT ACAAGTGCCA TGCCCTTGAA TGTTGCATCA 
TCAGCTGGCA GTGTAAGCGA CAGTTTTAAG CAGTTTACTG GTCCAGAGAC ATCTGATTTG 
GAGTCGACTG TAACGTCAAG TGTCAAGAGG CCTAAAATTG AGCTCTTCTT GCAGGCTAAT 
CATGCCCTTT TGGAAGAGAT AAGGGAAATA AATCAGCGAC TTTTAGACAC AGTGGTTGAT 
ATTAGTGATG AAGATGTTGA CTCAACTGCA GCAGCAGCTG CTGCTGAAGG GGGTGGAGGA 
ACCTTTGTCA AATGCTCATT TATCGCTGTG GCTCTGAGTC CAAACTTGAA AGCACAGTAC 
ACTTCAGCGC AAATGTCAAC AATTCAGCCC CTTAGATTGC TTGTGCCTAC AAATTATCCG 
AATTGCTCAC CGATACTGTT AGACAAGTTT CCATTTGAAG TCAGTAAAGA GTACGAGGAT 
TTGTCAATAA AAGCTAAATT TAGGTTTAGC ATCTCTCTTC GAAGCCTTTC ACAGCCCATG 
TCGCTTGGAG ACATAGCCAG GACTTGGGAT GTCTGTGCCC ATGCTGTTAT TTCAGAGCAT 
GCACAACAAA GTGGCGGAGG CACCTTCAGC TCAAAATATG GTTCTTGGGA AAACTGCGTG 
AGTGCAGCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POPTR_0001s21200g ( XM_024593686.1 ) cDNA ORF clone, Populus trichocarpa(Populus balsamifera subsp. trichocarpa) -> XP_024449454.1 Populus trichocarpa mediator of RNA polymerase II transcription subunit 15a (LOC7470541), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OPa423154
Clone ID Related Accession (Same CDS sequence)	XM_024593686.1
Accession Version	XM_024593686.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-08
Organism	Populus trichocarpa(black cottonwood)
Product	mediator of RNA polymerase II transcription subunit 15a isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037285.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCCA ATAATTGGAG GCCTACTGCC CCCGGTGGAG AACCTGTCAT GGACACGGGT 
GATTGGAGAA CTCAATTGCA GCCGGACGCA CGCCAAAGAA TAGTCAACAA AATCATGGAG 
ACATTAAAAA GACATCTTCC ATTTTCTGGT CAAGAGGGAT TACAAGAACT CAAGAAAATA 
GCCGTACGGT TTGAGGAAAA GATTTATACT GCTGCAACAA GTCAGTCTGA TTATCTGCGT 
AAAATATCTC TGAAGATGCT TGCAATGGAG AACAAGTCTC AAAGTACCAT ACCCAATTCT 
TTGCCACCCA ACTCCACTGG CAGTGGTAAC AAACCCCTGG ATCCAGGAGG ATCTCACAGT 
ATGCAGTCTC AAGTTCACAA TCAAGGGCCA TCACTCACTA TCCCATTGCC TGCTAATCAA 
TCTCAAGAAC GCCAGCAATT GTTGTCACAG AACATGCAGA CTGGTATGGC ATCTAGTGGA 
GTTCAAAGTT CTTCTGGTTT AACGTCAGCA TTGCCTCCCA TCTCCAGTTT AACCCAGACT 
GTCCCCAACT CTGTTGGCCA GAATCCTAAC ATGCAGAGTA TCTCTGGTGT TTCACAGAAC 
CCAGTGGGGA ATCCAATGGG ACAAGGGGTG CCCTCCAATA TGCTTGCCAA TTCTCAGAGA 
CAAGGTAGGC AACTAGTTGT TCCACAGCAA CAACAGCAGC CCCAAAATCC TCAGCAGTAT 
TTTTACCAGC AGCAGTTACA ACAACAGCTT TTGAAGCAGA AGCTTCAACA GGGAAATCCC 
CAGCACTCAC TTGTGCAATC TCACGTCCAG CAGCAGCAAC AGCAGCAGCA AAACCTATTA 
CAGTCAAATC AGTTGCAATC TTCTCAGCAG TCTGGTGTGC AAACACCATC TGTTATGCAG 
CCTTCAATAA TGCAAACGGC TCCTCTCTCT TGTCTTCAAC AGAATCAGCC ATCTTCTGTT 
CAACAGTCAA CACAACCCAT GCTTCAGCAA CATCCACAAC CAGTCCTCAG GCAGCAGCAA 
CAACCTCAAC AGACTGCTGG TATTCATCAG CAGCAGAGGT TGCTAGGCCA GCAGAATAAC 
CTTCCAAATC TGCAGCAGCA GCAATTAATG GTTCAACAAA ATAACCTATC AAGCATGCAT 
CAGCAACAGC TGGGCTCTCA AAGTAATGTC TCCAGTTTAC AGCAGCAGCA GCTGCTTGGA 
GCTCAGTCTG GTAACTCAAG CATGCAAACT AATCAGCACC CTGTACACAT GCTACAACAG 
TCCAAGGTTA CGCTGCAGCA ACAAGCACAA CAAAGTGCAG GTACTTTGTT ACCTAATCAA 
GGGCAGCAGT CACAACCACA GCTGCCACAG CAGCAATTGA TGTCACAGAT CCAATCACAG 
CCAGTGCAGC TGCAACAGCA ATCAAATCCA TTACAACATG ATCTGCAACA AAGATTTCAA 
GCATCAGGTT CCTTGCTTCA ACAGCAGAAT GTGACTGATC AGCAAAAGCA GTTATATCAA 
TCTCAAAGAG CCCTTCCAGA AACGTCCCCA ACATCCCTAG ATTCTACAAC ACAGACTGGA 
CATGTGAATG TCAATGATTG GCAAGAGGAG ATCTACCAAA AGATCAAAGT CATGAAGGAG 
ATGTATTTCC CTGAAATAAA TGAAATATAC CAGAGAATTG CTGCCAAGTT ACCGCAGCAT 
GACTCTCATC CGCAACAACC AAAGTCAGAA CAGCTTGACA AGCTAAAGGC ACTTAAGACC 
ATGTTGGAGC GCCTGATAAT GTTCTTACAA GTTCCGAAAA ACAACATCAA GCTTAATTTC 
AAAGAGAAGT TGGGTTACTA TGAGAATCAG ATTTTAAATT TTCTAAACAC AAGCAGACCC 
AGAAAGCCTG TTCCTAATCT TCAGCAAGGA CAGCTTCCCC AGCTTCATAT GCAACCTATG 
CAGCGACCCC AATCTCAAGT TCCTCAATTG CAGTCCCATG AAAATCAACT CAACCCCCAG 
TTGCAATCAA TGAATTTGCA AGGTTCTGTA CCAACAATGC AGCAGAACAA TGTGCCAAGC 
TTGCCGCATA ATTCTCTTTC TTCTTTATCT GGGGTTTCTA CATCACAACC AAACAAGATG 
AATCCAATGC AATCAGCTTC CAATTTAGAT TCTGGACAAG GAAATTCACT GAGCTCATTG 
CAGCAGGCAC CTGTAGGATC TGTACAACAG AATCCTGTGA GCAGTTCCCA ACCTACCAAT 
TTTAACACAT TGTCAACACA AAGTGGGGTG AGTATGCTGC AGTCGAATAT TCCTCTTCAG 
TTGAATTCCA ATATGATTCA ACAGCAGCAT CTAAAACAGC AGATGCTACA AACACAACAG 
CTTAAACAAC AGTTCCAACA GCGTCAGCCG CAGCAGCAAC AGCGTCAGAC GCAGCAGCAA 
CAGCATTTAA TGCAGAAGCA ACAGATGCTG CTGCAGCAGC AGCAGCAGCA GTTACACCAG 
CAAGCCAAGC AGCAGTTGCC TGCACAGATG CAGACACAAC AAATTCCACA GCCGCATCAG 
ATGAATGATG TAAATGAGAT GAAGTTGAGA CAGGGGATTG GAATCAAGCC AGCAGTCTTC 
CAACAACATC TCTCGACAGG TCAACGCACT GCTTTCCCTC ATCAACAAAT GAAACCAGGA 
TCCTCATTTC CTATTTCTTC ACCACAAATG CTCCAGCATG CTTCCCCCCA ATTACAACAA 
CATTCTTCTC CTCAGATTGA CCAGCAAAAT CTACTGCCTT CTCTAACTAA AACTGGAACA 
CCTTTGCAAT CTGCAAACTC GCCTTTTGTT GTCCCATCTC CTTCAACTCC TTTAGCTCCA 
TCCCCTGTGC CAGGAGATTC TGAGAAACCC ATTTCTGGTA TCTCCTCAAA TATTGTACAC 
CAACCAACTG TTGCACAAGC AACGGCTCCA TCCCTTGCAA TTGGCACTCC TGGGATATCT 
GCCTCGCCTT TGCTTGCAGA GTTTACCAGT CCTGATGGTG CTCATGGTGG TGCTTTGACA 
ACTGTTTCTG GCAAGTCGAA TGTTATGGAG CAACCACTTG AACGCATGAT TAAAGCGGTG 
AAATCTCTTT CTCCTAAAGC ACTTAGTGCA TCTGTCAGTG ACATTGGCTC TGTTGTCAGT 
ATGATTGACA GAATTGCTGG CTCGGCACCC GGTAATGGGT CTAGAGCTGC AGTTGGTGAG 
GATCTGGTAG CGATGACAAA ATGTCGCCTT CAGGCAAGAA ATTTCTTCAC CCAAGATGGG 
ATGACTGGAT CGAAGAAAAT GAGGCGCCAT ACAAGTGCCA TGCCCTTGAA TGTTGCATCA 
TCAGCTGGCA GTGTAAGCGA CAGTTTTAAG CAGTTTACTG GTCCAGAGAC ATCTGATTTG 
GAGTCGACTG TAACGTCAAG TGTCAAGAGG CCTAAAATTG AGGCTAATCA TGCCCTTTTG 
GAAGAGATAA GGGAAATAAA TCAGCGACTT TTAGACACAG TGGTTGATAT TAGTGATGAA 
GATGTTGACT CAACTGCAGC AGCAGCTGCT GCTGAAGGGG GTGGAGGAAC CTTTGTCAAA 
TGCTCATTTA TCGCTGTGGC TCTGAGTCCA AACTTGAAAG CACAGTACAC TTCAGCGCAA 
ATGTCAACAA TTCAGCCCCT TAGATTGCTT GTGCCTACAA ATTATCCGAA TTGCTCACCG 
ATACTGTTAG ACAAGTTTCC ATTTGAAGTC AGTAAAGAGT ACGAGGATTT GTCAATAAAA 
GCTAAATTTA GGTTTAGCAT CTCTCTTCGA AGCCTTTCAC AGCCCATGTC GCTTGGAGAC 
ATAGCCAGGA CTTGGGATGT CTGTGCCCAT GCTGTTATTT CAGAGCATGC ACAACAAAGT 
GGCGGAGGCA CCTTCAGCTC AAAATATGGT TCTTGGGAAA ACTGCGTGAG TGCAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024449454.1
CDS	216..4115
Translation	MDANNWRPTAPGGEPVMDTGDWRTQLQPDARQRIVNKIMETLKRHLPFSGQEGLQELKKIAVRFEEKIYTAATSQSDYLRKISLKMLAMENKSQSTIPNSLPPNSTGSGNKPLDPGGSHSMQSQVHNQGPSLTIPLPANQSQERQQLLSQNMQTGMASSGVQSSSGLTSALPPISSLTQTVPNSVGQNPNMQSISGVSQNPVGNPMGQGVPSNMLANSQRQGRQLVVPQQQQQPQNPQQYFYQQQLQQQLLKQKLQQGNPQHSLVQSHVQQQQQQQQNLLQSNQLQSSQQSGVQTPSVMQPSIMQTAPLSCLQQNQPSSVQQSTQPMLQQHPQPVLRQQQQPQQTAGIHQQQRLLGQQNNLPNLQQQQLMVQQNNLSSMHQQQLGSQSNVSSLQQQQLLGAQSGNSSMQTNQHPVHMLQQSKVTLQQQAQQSAGTLLPNQGQQSQPQLPQQQLMSQIQSQPVQLQQQSNPLQHDLQQRFQASGSLLQQQNVTDQQKQLYQSQRALPETSPTSLDSTTQTGHVNVNDWQEEIYQKIKVMKEMYFPEINEIYQRIAAKLPQHDSHPQQPKSEQLDKLKALKTMLERLIMFLQVPKNNIKLNFKEKLGYYENQILNFLNTSRPRKPVPNLQQGQLPQLHMQPMQRPQSQVPQLQSHENQLNPQLQSMNLQGSVPTMQQNNVPSLPHNSLSSLSGVSTSQPNKMNPMQSASNLDSGQGNSLSSLQQAPVGSVQQNPVSSSQPTNFNTLSTQSGVSMLQSNIPLQLNSNMIQQQHLKQQMLQTQQLKQQFQQRQPQQQQRQTQQQQHLMQKQQMLLQQQQQQLHQQAKQQLPAQMQTQQIPQPHQMNDVNEMKLRQGIGIKPAVFQQHLSTGQRTAFPHQQMKPGSSFPISSPQMLQHASPQLQQHSSPQIDQQNLLPSLTKTGTPLQSANSPFVVPSPSTPLAPSPVPGDSEKPISGISSNIVHQPTVAQATAPSLAIGTPGISASPLLAEFTSPDGAHGGALTTVSGKSNVMEQPLERMIKAVKSLSPKALSASVSDIGSVVSMIDRIAGSAPGNGSRAAVGEDLVAMTKCRLQARNFFTQDGMTGSKKMRRHTSAMPLNVASSAGSVSDSFKQFTGPETSDLESTVTSSVKRPKIEANHALLEEIREINQRLLDTVVDISDEDVDSTAAAAAAEGGGGTFVKCSFIAVALSPNLKAQYTSAQMSTIQPLRLLVPTNYPNCSPILLDKFPFEVSKEYEDLSIKAKFRFSISLRSLSQPMSLGDIARTWDVCAHAVISEHAQQSGGGTFSSKYGSWENCVSAA

Target ORF information:

RefSeq Version	XM_024593686.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa mediator of RNA polymerase II transcription subunit 15a (LOC7470541), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024593686.1

ORF Insert Sequence:

ATGGATGCCA ATAATTGGAG GCCTACTGCC CCCGGTGGAG AACCTGTCAT GGACACGGGT 
GATTGGAGAA CTCAATTGCA GCCGGACGCA CGCCAAAGAA TAGTCAACAA AATCATGGAG 
ACATTAAAAA GACATCTTCC ATTTTCTGGT CAAGAGGGAT TACAAGAACT CAAGAAAATA 
GCCGTACGGT TTGAGGAAAA GATTTATACT GCTGCAACAA GTCAGTCTGA TTATCTGCGT 
AAAATATCTC TGAAGATGCT TGCAATGGAG AACAAGTCTC AAAGTACCAT ACCCAATTCT 
TTGCCACCCA ACTCCACTGG CAGTGGTAAC AAACCCCTGG ATCCAGGAGG ATCTCACAGT 
ATGCAGTCTC AAGTTCACAA TCAAGGGCCA TCACTCACTA TCCCATTGCC TGCTAATCAA 
TCTCAAGAAC GCCAGCAATT GTTGTCACAG AACATGCAGA CTGGTATGGC ATCTAGTGGA 
GTTCAAAGTT CTTCTGGTTT AACGTCAGCA TTGCCTCCCA TCTCCAGTTT AACCCAGACT 
GTCCCCAACT CTGTTGGCCA GAATCCTAAC ATGCAGAGTA TCTCTGGTGT TTCACAGAAC 
CCAGTGGGGA ATCCAATGGG ACAAGGGGTG CCCTCCAATA TGCTTGCCAA TTCTCAGAGA 
CAAGGTAGGC AACTAGTTGT TCCACAGCAA CAACAGCAGC CCCAAAATCC TCAGCAGTAT 
TTTTACCAGC AGCAGTTACA ACAACAGCTT TTGAAGCAGA AGCTTCAACA GGGAAATCCC 
CAGCACTCAC TTGTGCAATC TCACGTCCAG CAGCAGCAAC AGCAGCAGCA AAACCTATTA 
CAGTCAAATC AGTTGCAATC TTCTCAGCAG TCTGGTGTGC AAACACCATC TGTTATGCAG 
CCTTCAATAA TGCAAACGGC TCCTCTCTCT TGTCTTCAAC AGAATCAGCC ATCTTCTGTT 
CAACAGTCAA CACAACCCAT GCTTCAGCAA CATCCACAAC CAGTCCTCAG GCAGCAGCAA 
CAACCTCAAC AGACTGCTGG TATTCATCAG CAGCAGAGGT TGCTAGGCCA GCAGAATAAC 
CTTCCAAATC TGCAGCAGCA GCAATTAATG GTTCAACAAA ATAACCTATC AAGCATGCAT 
CAGCAACAGC TGGGCTCTCA AAGTAATGTC TCCAGTTTAC AGCAGCAGCA GCTGCTTGGA 
GCTCAGTCTG GTAACTCAAG CATGCAAACT AATCAGCACC CTGTACACAT GCTACAACAG 
TCCAAGGTTA CGCTGCAGCA ACAAGCACAA CAAAGTGCAG GTACTTTGTT ACCTAATCAA 
GGGCAGCAGT CACAACCACA GCTGCCACAG CAGCAATTGA TGTCACAGAT CCAATCACAG 
CCAGTGCAGC TGCAACAGCA ATCAAATCCA TTACAACATG ATCTGCAACA AAGATTTCAA 
GCATCAGGTT CCTTGCTTCA ACAGCAGAAT GTGACTGATC AGCAAAAGCA GTTATATCAA 
TCTCAAAGAG CCCTTCCAGA AACGTCCCCA ACATCCCTAG ATTCTACAAC ACAGACTGGA 
CATGTGAATG TCAATGATTG GCAAGAGGAG ATCTACCAAA AGATCAAAGT CATGAAGGAG 
ATGTATTTCC CTGAAATAAA TGAAATATAC CAGAGAATTG CTGCCAAGTT ACCGCAGCAT 
GACTCTCATC CGCAACAACC AAAGTCAGAA CAGCTTGACA AGCTAAAGGC ACTTAAGACC 
ATGTTGGAGC GCCTGATAAT GTTCTTACAA GTTCCGAAAA ACAACATCAA GCTTAATTTC 
AAAGAGAAGT TGGGTTACTA TGAGAATCAG ATTTTAAATT TTCTAAACAC AAGCAGACCC 
AGAAAGCCTG TTCCTAATCT TCAGCAAGGA CAGCTTCCCC AGCTTCATAT GCAACCTATG 
CAGCGACCCC AATCTCAAGT TCCTCAATTG CAGTCCCATG AAAATCAACT CAACCCCCAG 
TTGCAATCAA TGAATTTGCA AGGTTCTGTA CCAACAATGC AGCAGAACAA TGTGCCAAGC 
TTGCCGCATA ATTCTCTTTC TTCTTTATCT GGGGTTTCTA CATCACAACC AAACAAGATG 
AATCCAATGC AATCAGCTTC CAATTTAGAT TCTGGACAAG GAAATTCACT GAGCTCATTG 
CAGCAGGCAC CTGTAGGATC TGTACAACAG AATCCTGTGA GCAGTTCCCA ACCTACCAAT 
TTTAACACAT TGTCAACACA AAGTGGGGTG AGTATGCTGC AGTCGAATAT TCCTCTTCAG 
TTGAATTCCA ATATGATTCA ACAGCAGCAT CTAAAACAGC AGATGCTACA AACACAACAG 
CTTAAACAAC AGTTCCAACA GCGTCAGCCG CAGCAGCAAC AGCGTCAGAC GCAGCAGCAA 
CAGCATTTAA TGCAGAAGCA ACAGATGCTG CTGCAGCAGC AGCAGCAGCA GTTACACCAG 
CAAGCCAAGC AGCAGTTGCC TGCACAGATG CAGACACAAC AAATTCCACA GCCGCATCAG 
ATGAATGATG TAAATGAGAT GAAGTTGAGA CAGGGGATTG GAATCAAGCC AGCAGTCTTC 
CAACAACATC TCTCGACAGG TCAACGCACT GCTTTCCCTC ATCAACAAAT GAAACCAGGA 
TCCTCATTTC CTATTTCTTC ACCACAAATG CTCCAGCATG CTTCCCCCCA ATTACAACAA 
CATTCTTCTC CTCAGATTGA CCAGCAAAAT CTACTGCCTT CTCTAACTAA AACTGGAACA 
CCTTTGCAAT CTGCAAACTC GCCTTTTGTT GTCCCATCTC CTTCAACTCC TTTAGCTCCA 
TCCCCTGTGC CAGGAGATTC TGAGAAACCC ATTTCTGGTA TCTCCTCAAA TATTGTACAC 
CAACCAACTG TTGCACAAGC AACGGCTCCA TCCCTTGCAA TTGGCACTCC TGGGATATCT 
GCCTCGCCTT TGCTTGCAGA GTTTACCAGT CCTGATGGTG CTCATGGTGG TGCTTTGACA 
ACTGTTTCTG GCAAGTCGAA TGTTATGGAG CAACCACTTG AACGCATGAT TAAAGCGGTG 
AAATCTCTTT CTCCTAAAGC ACTTAGTGCA TCTGTCAGTG ACATTGGCTC TGTTGTCAGT 
ATGATTGACA GAATTGCTGG CTCGGCACCC GGTAATGGGT CTAGAGCTGC AGTTGGTGAG 
GATCTGGTAG CGATGACAAA ATGTCGCCTT CAGGCAAGAA ATTTCTTCAC CCAAGATGGG 
ATGACTGGAT CGAAGAAAAT GAGGCGCCAT ACAAGTGCCA TGCCCTTGAA TGTTGCATCA 
TCAGCTGGCA GTGTAAGCGA CAGTTTTAAG CAGTTTACTG GTCCAGAGAC ATCTGATTTG 
GAGTCGACTG TAACGTCAAG TGTCAAGAGG CCTAAAATTG AGGCTAATCA TGCCCTTTTG 
GAAGAGATAA GGGAAATAAA TCAGCGACTT TTAGACACAG TGGTTGATAT TAGTGATGAA 
GATGTTGACT CAACTGCAGC AGCAGCTGCT GCTGAAGGGG GTGGAGGAAC CTTTGTCAAA 
TGCTCATTTA TCGCTGTGGC TCTGAGTCCA AACTTGAAAG CACAGTACAC TTCAGCGCAA 
ATGTCAACAA TTCAGCCCCT TAGATTGCTT GTGCCTACAA ATTATCCGAA TTGCTCACCG 
ATACTGTTAG ACAAGTTTCC ATTTGAAGTC AGTAAAGAGT ACGAGGATTT GTCAATAAAA 
GCTAAATTTA GGTTTAGCAT CTCTCTTCGA AGCCTTTCAC AGCCCATGTC GCTTGGAGAC 
ATAGCCAGGA CTTGGGATGT CTGTGCCCAT GCTGTTATTT CAGAGCATGC ACAACAAAGT 
GGCGGAGGCA CCTTCAGCTC AAAATATGGT TCTTGGGAAA ACTGCGTGAG TGCAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POPTR_0001s21200g ( XM_024593687.1 ) cDNA ORF clone, Populus trichocarpa(Populus balsamifera subsp. trichocarpa) -> XP_024449455.1 Populus trichocarpa mediator of RNA polymerase II transcription subunit 15a (LOC7470541), transcript variant X5, mRNA.

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CloneID	OPa423155
Clone ID Related Accession (Same CDS sequence)	XM_024593687.1
Accession Version	XM_024593687.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3837bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-08
Organism	Populus trichocarpa(black cottonwood)
Product	mediator of RNA polymerase II transcription subunit 15a isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037285.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCCA ATAATTGGAG GCCTACTGCC CCCGGTGGAG AACCTGTCAT GGACACGGGT 
GATTGGAGAA CTCAATTGCA GCCGGACGCA CGCCAAAGAA TAGTCAACAA AATCATGGAG 
ACATTAAAAA GACATCTTCC ATTTTCTGGT CAAGAGGGAT TACAAGAACT CAAGAAAATA 
GCCGTACGGT TTGAGGAAAA GATTTATACT GCTGCAACAA GTCAGTCTGA TTATCTGCGT 
AAAATATCTC TGAAGATGCT TGCAATGGAG AACAAGTCTC AAAGTACCAT ACCCAATTCT 
TTGCCACCCA ACTCCACTGG CAGTGGTAAC AAACCCCTGG ATCCAGGAGG ATCTCACAGT 
ATGCAGTCTC AAGTTCACAA TCAAGGGCCA TCACTCACTA TCCCATTGCC TGCTAATCAA 
TCTCAAGAAC GCCAGCAATT GTTGTCACAG AACATGCAGA CTGGTATGGC ATCTAGTGGA 
GTTCAAAGTT CTTCTGGTTT AACGTCAGCA TTGCCTCCCA TCTCCAGTTT AACCCAGACT 
GTCCCCAACT CTGTTGGCCA GAATCCTAAC ATGCAGAGTA TCTCTGGTGT TTCACAGAAC 
CCAGTGGGGA ATCCAATGGG ACAAGGGGTG CCCTCCAATA TGCTTGCCAA TTCTCAGAGA 
CAAGGTAGGC AACTAGTTGT TCCACAGCAA CAACAGCAGC CCCAAAATCC TCAGCAGTAT 
TTTTACCAGC AGCAGTTACA ACAACAGCTT TTGAAGCAGA AGCTTCAACA GGGAAATCCC 
CAGCACTCAC TTGTGCAATC TCACGTCCAG CAGCAGCAAC AGCAGCAGCA AAACCTATTA 
CAGTCAAATC AGTTGCAATC TTCTCAGCAG TCTGGTGTGC AAACACCATC TGTTATGCAG 
CCTTCAATAA TGCAAACGGC TCCTCTCTCT TGTCTTCAAC AGAATCAGCC ATCTTCTGTT 
CAACAGTCAA CACAACCCAT GCTTCAGCAA CATCCACAAC CAGTCCTCAG GCAGCAGCAA 
CAACCTCAAC AGACTGCTGG TATTCATCAG CAGCAGAGGT TGCTAGGCCA GCAGAATAAC 
CTTCCAAATC TGCAGCAGCA GCAATTAATG GTTCAACAAA ATAACCTATC AAGCATGCAT 
CAGCAACAGC TGGGCTCTCA AAGTAATGTC TCCAGTTTAC AGCAGCAGCA GCTGCTTGGA 
GCTCAGTCTG GTAACTCAAG CATGCAAACT AATCAGCACC CTGTACACAT GCTACAACAG 
TCCAAGGTTA CGCTGCAGCA ACAAGCACAA CAAAGTGCAG GTACTTTGTT ACCTAATCAA 
GGGCAGCAGT CACAACCACA GCTGCCACAG CAGCAATTGA TGTCACAGAT CCAATCACAG 
CCAGTGCAGC TGCAACAGCA ATCAAATCCA TTACAACATG ATCTGCAACA AAGATTTCAA 
GCATCAGGTT CCTTGCTTCA ACAGCAGAAT GTGACTGATC AGCAAAAGCA GTTATATCAA 
TCTCAAAGAG CCCTTCCAGA AACGTCCCCA ACATCCCTAG ATTCTACAAC ACAGACTGGA 
CATGTGAATG TCAATGATTG GCAAGAGGAG ATCTACCAAA AGCATGACTC TCATCCGCAA 
CAACCAAAGT CAGAACAGCT TGACAAGCTA AAGGCACTTA AGACCATGTT GGAGCGCCTG 
ATAATGTTCT TACAAGTTCC GAAAAACAAC ATCAAGCTTA ATTTCAAAGA GAAGTTGGGT 
TACTATGAGA ATCAGATTTT AAATTTTCTA AACACAAGCA GACCCAGAAA GCCTGTTCCT 
AATCTTCAGC AAGGACAGCT TCCCCAGCTT CATATGCAAC CTATGCAGCG ACCCCAATCT 
CAAGTTCCTC AATTGCAGTC CCATGAAAAT CAACTCAACC CCCAGTTGCA ATCAATGAAT 
TTGCAAGGTT CTGTACCAAC AATGCAGCAG AACAATGTGC CAAGCTTGCC GCATAATTCT 
CTTTCTTCTT TATCTGGGGT TTCTACATCA CAACCAAACA AGATGAATCC AATGCAATCA 
GCTTCCAATT TAGATTCTGG ACAAGGAAAT TCACTGAGCT CATTGCAGCA GGCACCTGTA 
GGATCTGTAC AACAGAATCC TGTGAGCAGT TCCCAACCTA CCAATTTTAA CACATTGTCA 
ACACAAAGTG GGGTGAGTAT GCTGCAGTCG AATATTCCTC TTCAGTTGAA TTCCAATATG 
ATTCAACAGC AGCATCTAAA ACAGCAGATG CTACAAACAC AACAGCTTAA ACAACAGTTC 
CAACAGCGTC AGCCGCAGCA GCAACAGCGT CAGACGCAGC AGCAACAGCA TTTAATGCAG 
AAGCAACAGA TGCTGCTGCA GCAGCAGCAG CAGCAGTTAC ACCAGCAAGC CAAGCAGCAG 
TTGCCTGCAC AGATGCAGAC ACAACAAATT CCACAGCCGC ATCAGATGAA TGATGTAAAT 
GAGATGAAGT TGAGACAGGG GATTGGAATC AAGCCAGCAG TCTTCCAACA ACATCTCTCG 
ACAGGTCAAC GCACTGCTTT CCCTCATCAA CAAATGAAAC CAGGATCCTC ATTTCCTATT 
TCTTCACCAC AAATGCTCCA GCATGCTTCC CCCCAATTAC AACAACATTC TTCTCCTCAG 
ATTGACCAGC AAAATCTACT GCCTTCTCTA ACTAAAACTG GAACACCTTT GCAATCTGCA 
AACTCGCCTT TTGTTGTCCC ATCTCCTTCA ACTCCTTTAG CTCCATCCCC TGTGCCAGGA 
GATTCTGAGA AACCCATTTC TGGTATCTCC TCAAATATTG TACACCAACC AACTGTTGCA 
CAAGCAACGG CTCCATCCCT TGCAATTGGC ACTCCTGGGA TATCTGCCTC GCCTTTGCTT 
GCAGAGTTTA CCAGTCCTGA TGGTGCTCAT GGTGGTGCTT TGACAACTGT TTCTGGCAAG 
TCGAATGTTA TGGAGCAACC ACTTGAACGC ATGATTAAAG CGGTGAAATC TCTTTCTCCT 
AAAGCACTTA GTGCATCTGT CAGTGACATT GGCTCTGTTG TCAGTATGAT TGACAGAATT 
GCTGGCTCGG CACCCGGTAA TGGGTCTAGA GCTGCAGTTG GTGAGGATCT GGTAGCGATG 
ACAAAATGTC GCCTTCAGGC AAGAAATTTC TTCACCCAAG ATGGGATGAC TGGATCGAAG 
AAAATGAGGC GCCATACAAG TGCCATGCCC TTGAATGTTG CATCATCAGC TGGCAGTGTA 
AGCGACAGTT TTAAGCAGTT TACTGGTCCA GAGACATCTG ATTTGGAGTC GACTGTAACG 
TCAAGTGTCA AGAGGCCTAA AATTGAGCTC TTCTTGCAGG CTAATCATGC CCTTTTGGAA 
GAGATAAGGG AAATAAATCA GCGACTTTTA GACACAGTGG TTGATATTAG TGATGAAGAT 
GTTGACTCAA CTGCAGCAGC AGCTGCTGCT GAAGGGGGTG GAGGAACCTT TGTCAAATGC 
TCATTTATCG CTGTGGCTCT GAGTCCAAAC TTGAAAGCAC AGTACACTTC AGCGCAAATG 
TCAACAATTC AGCCCCTTAG ATTGCTTGTG CCTACAAATT ATCCGAATTG CTCACCGATA 
CTGTTAGACA AGTTTCCATT TGAAGTCAGT AAAGAGTACG AGGATTTGTC AATAAAAGCT 
AAATTTAGGT TTAGCATCTC TCTTCGAAGC CTTTCACAGC CCATGTCGCT TGGAGACATA 
GCCAGGACTT GGGATGTCTG TGCCCATGCT GTTATTTCAG AGCATGCACA ACAAAGTGGC 
GGAGGCACCT TCAGCTCAAA ATATGGTTCT TGGGAAAACT GCGTGAGTGC AGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024449455.1
CDS	217..4053
Translation	MDANNWRPTAPGGEPVMDTGDWRTQLQPDARQRIVNKIMETLKRHLPFSGQEGLQELKKIAVRFEEKIYTAATSQSDYLRKISLKMLAMENKSQSTIPNSLPPNSTGSGNKPLDPGGSHSMQSQVHNQGPSLTIPLPANQSQERQQLLSQNMQTGMASSGVQSSSGLTSALPPISSLTQTVPNSVGQNPNMQSISGVSQNPVGNPMGQGVPSNMLANSQRQGRQLVVPQQQQQPQNPQQYFYQQQLQQQLLKQKLQQGNPQHSLVQSHVQQQQQQQQNLLQSNQLQSSQQSGVQTPSVMQPSIMQTAPLSCLQQNQPSSVQQSTQPMLQQHPQPVLRQQQQPQQTAGIHQQQRLLGQQNNLPNLQQQQLMVQQNNLSSMHQQQLGSQSNVSSLQQQQLLGAQSGNSSMQTNQHPVHMLQQSKVTLQQQAQQSAGTLLPNQGQQSQPQLPQQQLMSQIQSQPVQLQQQSNPLQHDLQQRFQASGSLLQQQNVTDQQKQLYQSQRALPETSPTSLDSTTQTGHVNVNDWQEEIYQKHDSHPQQPKSEQLDKLKALKTMLERLIMFLQVPKNNIKLNFKEKLGYYENQILNFLNTSRPRKPVPNLQQGQLPQLHMQPMQRPQSQVPQLQSHENQLNPQLQSMNLQGSVPTMQQNNVPSLPHNSLSSLSGVSTSQPNKMNPMQSASNLDSGQGNSLSSLQQAPVGSVQQNPVSSSQPTNFNTLSTQSGVSMLQSNIPLQLNSNMIQQQHLKQQMLQTQQLKQQFQQRQPQQQQRQTQQQQHLMQKQQMLLQQQQQQLHQQAKQQLPAQMQTQQIPQPHQMNDVNEMKLRQGIGIKPAVFQQHLSTGQRTAFPHQQMKPGSSFPISSPQMLQHASPQLQQHSSPQIDQQNLLPSLTKTGTPLQSANSPFVVPSPSTPLAPSPVPGDSEKPISGISSNIVHQPTVAQATAPSLAIGTPGISASPLLAEFTSPDGAHGGALTTVSGKSNVMEQPLERMIKAVKSLSPKALSASVSDIGSVVSMIDRIAGSAPGNGSRAAVGEDLVAMTKCRLQARNFFTQDGMTGSKKMRRHTSAMPLNVASSAGSVSDSFKQFTGPETSDLESTVTSSVKRPKIELFLQANHALLEEIREINQRLLDTVVDISDEDVDSTAAAAAAEGGGGTFVKCSFIAVALSPNLKAQYTSAQMSTIQPLRLLVPTNYPNCSPILLDKFPFEVSKEYEDLSIKAKFRFSISLRSLSQPMSLGDIARTWDVCAHAVISEHAQQSGGGTFSSKYGSWENCVSAA

Target ORF information:

RefSeq Version	XM_024593687.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa mediator of RNA polymerase II transcription subunit 15a (LOC7470541), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024593687.1

ORF Insert Sequence:

ATGGATGCCA ATAATTGGAG GCCTACTGCC CCCGGTGGAG AACCTGTCAT GGACACGGGT 
GATTGGAGAA CTCAATTGCA GCCGGACGCA CGCCAAAGAA TAGTCAACAA AATCATGGAG 
ACATTAAAAA GACATCTTCC ATTTTCTGGT CAAGAGGGAT TACAAGAACT CAAGAAAATA 
GCCGTACGGT TTGAGGAAAA GATTTATACT GCTGCAACAA GTCAGTCTGA TTATCTGCGT 
AAAATATCTC TGAAGATGCT TGCAATGGAG AACAAGTCTC AAAGTACCAT ACCCAATTCT 
TTGCCACCCA ACTCCACTGG CAGTGGTAAC AAACCCCTGG ATCCAGGAGG ATCTCACAGT 
ATGCAGTCTC AAGTTCACAA TCAAGGGCCA TCACTCACTA TCCCATTGCC TGCTAATCAA 
TCTCAAGAAC GCCAGCAATT GTTGTCACAG AACATGCAGA CTGGTATGGC ATCTAGTGGA 
GTTCAAAGTT CTTCTGGTTT AACGTCAGCA TTGCCTCCCA TCTCCAGTTT AACCCAGACT 
GTCCCCAACT CTGTTGGCCA GAATCCTAAC ATGCAGAGTA TCTCTGGTGT TTCACAGAAC 
CCAGTGGGGA ATCCAATGGG ACAAGGGGTG CCCTCCAATA TGCTTGCCAA TTCTCAGAGA 
CAAGGTAGGC AACTAGTTGT TCCACAGCAA CAACAGCAGC CCCAAAATCC TCAGCAGTAT 
TTTTACCAGC AGCAGTTACA ACAACAGCTT TTGAAGCAGA AGCTTCAACA GGGAAATCCC 
CAGCACTCAC TTGTGCAATC TCACGTCCAG CAGCAGCAAC AGCAGCAGCA AAACCTATTA 
CAGTCAAATC AGTTGCAATC TTCTCAGCAG TCTGGTGTGC AAACACCATC TGTTATGCAG 
CCTTCAATAA TGCAAACGGC TCCTCTCTCT TGTCTTCAAC AGAATCAGCC ATCTTCTGTT 
CAACAGTCAA CACAACCCAT GCTTCAGCAA CATCCACAAC CAGTCCTCAG GCAGCAGCAA 
CAACCTCAAC AGACTGCTGG TATTCATCAG CAGCAGAGGT TGCTAGGCCA GCAGAATAAC 
CTTCCAAATC TGCAGCAGCA GCAATTAATG GTTCAACAAA ATAACCTATC AAGCATGCAT 
CAGCAACAGC TGGGCTCTCA AAGTAATGTC TCCAGTTTAC AGCAGCAGCA GCTGCTTGGA 
GCTCAGTCTG GTAACTCAAG CATGCAAACT AATCAGCACC CTGTACACAT GCTACAACAG 
TCCAAGGTTA CGCTGCAGCA ACAAGCACAA CAAAGTGCAG GTACTTTGTT ACCTAATCAA 
GGGCAGCAGT CACAACCACA GCTGCCACAG CAGCAATTGA TGTCACAGAT CCAATCACAG 
CCAGTGCAGC TGCAACAGCA ATCAAATCCA TTACAACATG ATCTGCAACA AAGATTTCAA 
GCATCAGGTT CCTTGCTTCA ACAGCAGAAT GTGACTGATC AGCAAAAGCA GTTATATCAA 
TCTCAAAGAG CCCTTCCAGA AACGTCCCCA ACATCCCTAG ATTCTACAAC ACAGACTGGA 
CATGTGAATG TCAATGATTG GCAAGAGGAG ATCTACCAAA AGCATGACTC TCATCCGCAA 
CAACCAAAGT CAGAACAGCT TGACAAGCTA AAGGCACTTA AGACCATGTT GGAGCGCCTG 
ATAATGTTCT TACAAGTTCC GAAAAACAAC ATCAAGCTTA ATTTCAAAGA GAAGTTGGGT 
TACTATGAGA ATCAGATTTT AAATTTTCTA AACACAAGCA GACCCAGAAA GCCTGTTCCT 
AATCTTCAGC AAGGACAGCT TCCCCAGCTT CATATGCAAC CTATGCAGCG ACCCCAATCT 
CAAGTTCCTC AATTGCAGTC CCATGAAAAT CAACTCAACC CCCAGTTGCA ATCAATGAAT 
TTGCAAGGTT CTGTACCAAC AATGCAGCAG AACAATGTGC CAAGCTTGCC GCATAATTCT 
CTTTCTTCTT TATCTGGGGT TTCTACATCA CAACCAAACA AGATGAATCC AATGCAATCA 
GCTTCCAATT TAGATTCTGG ACAAGGAAAT TCACTGAGCT CATTGCAGCA GGCACCTGTA 
GGATCTGTAC AACAGAATCC TGTGAGCAGT TCCCAACCTA CCAATTTTAA CACATTGTCA 
ACACAAAGTG GGGTGAGTAT GCTGCAGTCG AATATTCCTC TTCAGTTGAA TTCCAATATG 
ATTCAACAGC AGCATCTAAA ACAGCAGATG CTACAAACAC AACAGCTTAA ACAACAGTTC 
CAACAGCGTC AGCCGCAGCA GCAACAGCGT CAGACGCAGC AGCAACAGCA TTTAATGCAG 
AAGCAACAGA TGCTGCTGCA GCAGCAGCAG CAGCAGTTAC ACCAGCAAGC CAAGCAGCAG 
TTGCCTGCAC AGATGCAGAC ACAACAAATT CCACAGCCGC ATCAGATGAA TGATGTAAAT 
GAGATGAAGT TGAGACAGGG GATTGGAATC AAGCCAGCAG TCTTCCAACA ACATCTCTCG 
ACAGGTCAAC GCACTGCTTT CCCTCATCAA CAAATGAAAC CAGGATCCTC ATTTCCTATT 
TCTTCACCAC AAATGCTCCA GCATGCTTCC CCCCAATTAC AACAACATTC TTCTCCTCAG 
ATTGACCAGC AAAATCTACT GCCTTCTCTA ACTAAAACTG GAACACCTTT GCAATCTGCA 
AACTCGCCTT TTGTTGTCCC ATCTCCTTCA ACTCCTTTAG CTCCATCCCC TGTGCCAGGA 
GATTCTGAGA AACCCATTTC TGGTATCTCC TCAAATATTG TACACCAACC AACTGTTGCA 
CAAGCAACGG CTCCATCCCT TGCAATTGGC ACTCCTGGGA TATCTGCCTC GCCTTTGCTT 
GCAGAGTTTA CCAGTCCTGA TGGTGCTCAT GGTGGTGCTT TGACAACTGT TTCTGGCAAG 
TCGAATGTTA TGGAGCAACC ACTTGAACGC ATGATTAAAG CGGTGAAATC TCTTTCTCCT 
AAAGCACTTA GTGCATCTGT CAGTGACATT GGCTCTGTTG TCAGTATGAT TGACAGAATT 
GCTGGCTCGG CACCCGGTAA TGGGTCTAGA GCTGCAGTTG GTGAGGATCT GGTAGCGATG 
ACAAAATGTC GCCTTCAGGC AAGAAATTTC TTCACCCAAG ATGGGATGAC TGGATCGAAG 
AAAATGAGGC GCCATACAAG TGCCATGCCC TTGAATGTTG CATCATCAGC TGGCAGTGTA 
AGCGACAGTT TTAAGCAGTT TACTGGTCCA GAGACATCTG ATTTGGAGTC GACTGTAACG 
TCAAGTGTCA AGAGGCCTAA AATTGAGCTC TTCTTGCAGG CTAATCATGC CCTTTTGGAA 
GAGATAAGGG AAATAAATCA GCGACTTTTA GACACAGTGG TTGATATTAG TGATGAAGAT 
GTTGACTCAA CTGCAGCAGC AGCTGCTGCT GAAGGGGGTG GAGGAACCTT TGTCAAATGC 
TCATTTATCG CTGTGGCTCT GAGTCCAAAC TTGAAAGCAC AGTACACTTC AGCGCAAATG 
TCAACAATTC AGCCCCTTAG ATTGCTTGTG CCTACAAATT ATCCGAATTG CTCACCGATA 
CTGTTAGACA AGTTTCCATT TGAAGTCAGT AAAGAGTACG AGGATTTGTC AATAAAAGCT 
AAATTTAGGT TTAGCATCTC TCTTCGAAGC CTTTCACAGC CCATGTCGCT TGGAGACATA 
GCCAGGACTT GGGATGTCTG TGCCCATGCT GTTATTTCAG AGCATGCACA ACAAAGTGGC 
GGAGGCACCT TCAGCTCAAA ATATGGTTCT TGGGAAAACT GCGTGAGTGC AGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POPTR_0001s21200g ( XM_024593688.1 ) cDNA ORF clone, Populus trichocarpa(Populus balsamifera subsp. trichocarpa) -> XP_024449456.1 Populus trichocarpa mediator of RNA polymerase II transcription subunit 15a (LOC7470541), transcript variant X6, mRNA.

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CloneID	OPa423156
Clone ID Related Accession (Same CDS sequence)	XM_024593688.1
Accession Version	XM_024593688.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3807bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-08
Organism	Populus trichocarpa(black cottonwood)
Product	mediator of RNA polymerase II transcription subunit 15a isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037285.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCAGCA TGGAGACATT AAAAAGACAT CTTCCATTTT CTGGTCAAGA GGGATTACAA 
GAACTCAAGA AAATAGCCGT ACGGTTTGAG GAAAAGATTT ATACTGCTGC AACAAGTCAG 
TCTGATTATC TGCGTAAAAT ATCTCTGAAG ATGCTTGCAA TGGAGAACAA GTCTCAAAGT 
ACCATACCCA ATTCTTTGCC ACCCAACTCC ACTGGCAGTG GTAACAAACC CCTGGATCCA 
GGAGGATCTC ACAGTATGCA GTCTCAAGTT CACAATCAAG GGCCATCACT CACTATCCCA 
TTGCCTGCTA ATCAATCTCA AGAACGCCAG CAATTGTTGT CACAGAACAT GCAGACTGGT 
ATGGCATCTA GTGGAGTTCA AAGTTCTTCT GGTTTAACGT CAGCATTGCC TCCCATCTCC 
AGTTTAACCC AGACTGTCCC CAACTCTGTT GGCCAGAATC CTAACATGCA GAGTATCTCT 
GGTGTTTCAC AGAACCCAGT GGGGAATCCA ATGGGACAAG GGGTGCCCTC CAATATGCTT 
GCCAATTCTC AGAGACAAGG TAGGCAACTA GTTGTTCCAC AGCAACAACA GCAGCCCCAA 
AATCCTCAGC AGTATTTTTA CCAGCAGCAG TTACAACAAC AGCTTTTGAA GCAGAAGCTT 
CAACAGGGAA ATCCCCAGCA CTCACTTGTG CAATCTCACG TCCAGCAGCA GCAACAGCAG 
CAGCAAAACC TATTACAGTC AAATCAGTTG CAATCTTCTC AGCAGTCTGG TGTGCAAACA 
CCATCTGTTA TGCAGCCTTC AATAATGCAA ACGGCTCCTC TCTCTTGTCT TCAACAGAAT 
CAGCCATCTT CTGTTCAACA GTCAACACAA CCCATGCTTC AGCAACATCC ACAACCAGTC 
CTCAGGCAGC AGCAACAACC TCAACAGACT GCTGGTATTC ATCAGCAGCA GAGGTTGCTA 
GGCCAGCAGA ATAACCTTCC AAATCTGCAG CAGCAGCAAT TAATGGTTCA ACAAAATAAC 
CTATCAAGCA TGCATCAGCA ACAGCTGGGC TCTCAAAGTA ATGTCTCCAG TTTACAGCAG 
CAGCAGCTGC TTGGAGCTCA GTCTGGTAAC TCAAGCATGC AAACTAATCA GCACCCTGTA 
CACATGCTAC AACAGTCCAA GGTTACGCTG CAGCAACAAG CACAACAAAG TGCAGGTACT 
TTGTTACCTA ATCAAGGGCA GCAGTCACAA CCACAGCTGC CACAGCAGCA ATTGATGTCA 
CAGATCCAAT CACAGCCAGT GCAGCTGCAA CAGCAATCAA ATCCATTACA ACATGATCTG 
CAACAAAGAT TTCAAGCATC AGGTTCCTTG CTTCAACAGC AGAATGTGAC TGATCAGCAA 
AAGCAGTTAT ATCAATCTCA AAGAGCCCTT CCAGAAACGT CCCCAACATC CCTAGATTCT 
ACAACACAGA CTGGACATGT GAATGTCAAT GATTGGCAAG AGGAGATCTA CCAAAAGATC 
AAAGTCATGA AGGAGATGTA TTTCCCTGAA ATAAATGAAA TATACCAGAG AATTGCTGCC 
AAGTTACCGC AGCATGACTC TCATCCGCAA CAACCAAAGT CAGAACAGCT TGACAAGCTA 
AAGGCACTTA AGACCATGTT GGAGCGCCTG ATAATGTTCT TACAAGTTCC GAAAAACAAC 
ATCAAGCTTA ATTTCAAAGA GAAGTTGGGT TACTATGAGA ATCAGATTTT AAATTTTCTA 
AACACAAGCA GACCCAGAAA GCCTGTTCCT AATCTTCAGC AAGGACAGCT TCCCCAGCTT 
CATATGCAAC CTATGCAGCG ACCCCAATCT CAAGTTCCTC AATTGCAGTC CCATGAAAAT 
CAACTCAACC CCCAGTTGCA ATCAATGAAT TTGCAAGGTT CTGTACCAAC AATGCAGCAG 
AACAATGTGC CAAGCTTGCC GCATAATTCT CTTTCTTCTT TATCTGGGGT TTCTACATCA 
CAACCAAACA AGATGAATCC AATGCAATCA GCTTCCAATT TAGATTCTGG ACAAGGAAAT 
TCACTGAGCT CATTGCAGCA GGCACCTGTA GGATCTGTAC AACAGAATCC TGTGAGCAGT 
TCCCAACCTA CCAATTTTAA CACATTGTCA ACACAAAGTG GGGTGAGTAT GCTGCAGTCG 
AATATTCCTC TTCAGTTGAA TTCCAATATG ATTCAACAGC AGCATCTAAA ACAGCAGATG 
CTACAAACAC AACAGCTTAA ACAACAGTTC CAACAGCGTC AGCCGCAGCA GCAACAGCGT 
CAGACGCAGC AGCAACAGCA TTTAATGCAG AAGCAACAGA TGCTGCTGCA GCAGCAGCAG 
CAGCAGTTAC ACCAGCAAGC CAAGCAGCAG TTGCCTGCAC AGATGCAGAC ACAACAAATT 
CCACAGCCGC ATCAGATGAA TGATGTAAAT GAGATGAAGT TGAGACAGGG GATTGGAATC 
AAGCCAGCAG TCTTCCAACA ACATCTCTCG ACAGGTCAAC GCACTGCTTT CCCTCATCAA 
CAAATGAAAC CAGGATCCTC ATTTCCTATT TCTTCACCAC AAATGCTCCA GCATGCTTCC 
CCCCAATTAC AACAACATTC TTCTCCTCAG ATTGACCAGC AAAATCTACT GCCTTCTCTA 
ACTAAAACTG GAACACCTTT GCAATCTGCA AACTCGCCTT TTGTTGTCCC ATCTCCTTCA 
ACTCCTTTAG CTCCATCCCC TGTGCCAGGA GATTCTGAGA AACCCATTTC TGGTATCTCC 
TCAAATATTG TACACCAACC AACTGTTGCA CAAGCAACGG CTCCATCCCT TGCAATTGGC 
ACTCCTGGGA TATCTGCCTC GCCTTTGCTT GCAGAGTTTA CCAGTCCTGA TGGTGCTCAT 
GGTGGTGCTT TGACAACTGT TTCTGGCAAG TCGAATGTTA TGGAGCAACC ACTTGAACGC 
ATGATTAAAG CGGTGAAATC TCTTTCTCCT AAAGCACTTA GTGCATCTGT CAGTGACATT 
GGCTCTGTTG TCAGTATGAT TGACAGAATT GCTGGCTCGG CACCCGGTAA TGGGTCTAGA 
GCTGCAGTTG GTGAGGATCT GGTAGCGATG ACAAAATGTC GCCTTCAGGC AAGAAATTTC 
TTCACCCAAG ATGGGATGAC TGGATCGAAG AAAATGAGGC GCCATACAAG TGCCATGCCC 
TTGAATGTTG CATCATCAGC TGGCAGTGTA AGCGACAGTT TTAAGCAGTT TACTGGTCCA 
GAGACATCTG ATTTGGAGTC GACTGTAACG TCAAGTGTCA AGAGGCCTAA AATTGAGCTC 
TTCTTGCAGG CTAATCATGC CCTTTTGGAA GAGATAAGGG AAATAAATCA GCGACTTTTA 
GACACAGTGG TTGATATTAG TGATGAAGAT GTTGACTCAA CTGCAGCAGC AGCTGCTGCT 
GAAGGGGGTG GAGGAACCTT TGTCAAATGC TCATTTATCG CTGTGGCTCT GAGTCCAAAC 
TTGAAAGCAC AGTACACTTC AGCGCAAATG TCAACAATTC AGCCCCTTAG ATTGCTTGTG 
CCTACAAATT ATCCGAATTG CTCACCGATA CTGTTAGACA AGTTTCCATT TGAAGTCAGT 
AAAGAGTACG AGGATTTGTC AATAAAAGCT AAATTTAGGT TTAGCATCTC TCTTCGAAGC 
CTTTCACAGC CCATGTCGCT TGGAGACATA GCCAGGACTT GGGATGTCTG TGCCCATGCT 
GTTATTTCAG AGCATGCACA ACAAAGTGGC GGAGGCACCT TCAGCTCAAA ATATGGTTCT 
TGGGAAAACT GCGTGAGTGC AGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024449456.1
CDS	146..3952
Translation	MCSMETLKRHLPFSGQEGLQELKKIAVRFEEKIYTAATSQSDYLRKISLKMLAMENKSQSTIPNSLPPNSTGSGNKPLDPGGSHSMQSQVHNQGPSLTIPLPANQSQERQQLLSQNMQTGMASSGVQSSSGLTSALPPISSLTQTVPNSVGQNPNMQSISGVSQNPVGNPMGQGVPSNMLANSQRQGRQLVVPQQQQQPQNPQQYFYQQQLQQQLLKQKLQQGNPQHSLVQSHVQQQQQQQQNLLQSNQLQSSQQSGVQTPSVMQPSIMQTAPLSCLQQNQPSSVQQSTQPMLQQHPQPVLRQQQQPQQTAGIHQQQRLLGQQNNLPNLQQQQLMVQQNNLSSMHQQQLGSQSNVSSLQQQQLLGAQSGNSSMQTNQHPVHMLQQSKVTLQQQAQQSAGTLLPNQGQQSQPQLPQQQLMSQIQSQPVQLQQQSNPLQHDLQQRFQASGSLLQQQNVTDQQKQLYQSQRALPETSPTSLDSTTQTGHVNVNDWQEEIYQKIKVMKEMYFPEINEIYQRIAAKLPQHDSHPQQPKSEQLDKLKALKTMLERLIMFLQVPKNNIKLNFKEKLGYYENQILNFLNTSRPRKPVPNLQQGQLPQLHMQPMQRPQSQVPQLQSHENQLNPQLQSMNLQGSVPTMQQNNVPSLPHNSLSSLSGVSTSQPNKMNPMQSASNLDSGQGNSLSSLQQAPVGSVQQNPVSSSQPTNFNTLSTQSGVSMLQSNIPLQLNSNMIQQQHLKQQMLQTQQLKQQFQQRQPQQQQRQTQQQQHLMQKQQMLLQQQQQQLHQQAKQQLPAQMQTQQIPQPHQMNDVNEMKLRQGIGIKPAVFQQHLSTGQRTAFPHQQMKPGSSFPISSPQMLQHASPQLQQHSSPQIDQQNLLPSLTKTGTPLQSANSPFVVPSPSTPLAPSPVPGDSEKPISGISSNIVHQPTVAQATAPSLAIGTPGISASPLLAEFTSPDGAHGGALTTVSGKSNVMEQPLERMIKAVKSLSPKALSASVSDIGSVVSMIDRIAGSAPGNGSRAAVGEDLVAMTKCRLQARNFFTQDGMTGSKKMRRHTSAMPLNVASSAGSVSDSFKQFTGPETSDLESTVTSSVKRPKIELFLQANHALLEEIREINQRLLDTVVDISDEDVDSTAAAAAAEGGGGTFVKCSFIAVALSPNLKAQYTSAQMSTIQPLRLLVPTNYPNCSPILLDKFPFEVSKEYEDLSIKAKFRFSISLRSLSQPMSLGDIARTWDVCAHAVISEHAQQSGGGTFSSKYGSWENCVSAA

Target ORF information:

RefSeq Version	XM_024593688.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa mediator of RNA polymerase II transcription subunit 15a (LOC7470541), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024593688.1

ORF Insert Sequence:

ATGTGCAGCA TGGAGACATT AAAAAGACAT CTTCCATTTT CTGGTCAAGA GGGATTACAA 
GAACTCAAGA AAATAGCCGT ACGGTTTGAG GAAAAGATTT ATACTGCTGC AACAAGTCAG 
TCTGATTATC TGCGTAAAAT ATCTCTGAAG ATGCTTGCAA TGGAGAACAA GTCTCAAAGT 
ACCATACCCA ATTCTTTGCC ACCCAACTCC ACTGGCAGTG GTAACAAACC CCTGGATCCA 
GGAGGATCTC ACAGTATGCA GTCTCAAGTT CACAATCAAG GGCCATCACT CACTATCCCA 
TTGCCTGCTA ATCAATCTCA AGAACGCCAG CAATTGTTGT CACAGAACAT GCAGACTGGT 
ATGGCATCTA GTGGAGTTCA AAGTTCTTCT GGTTTAACGT CAGCATTGCC TCCCATCTCC 
AGTTTAACCC AGACTGTCCC CAACTCTGTT GGCCAGAATC CTAACATGCA GAGTATCTCT 
GGTGTTTCAC AGAACCCAGT GGGGAATCCA ATGGGACAAG GGGTGCCCTC CAATATGCTT 
GCCAATTCTC AGAGACAAGG TAGGCAACTA GTTGTTCCAC AGCAACAACA GCAGCCCCAA 
AATCCTCAGC AGTATTTTTA CCAGCAGCAG TTACAACAAC AGCTTTTGAA GCAGAAGCTT 
CAACAGGGAA ATCCCCAGCA CTCACTTGTG CAATCTCACG TCCAGCAGCA GCAACAGCAG 
CAGCAAAACC TATTACAGTC AAATCAGTTG CAATCTTCTC AGCAGTCTGG TGTGCAAACA 
CCATCTGTTA TGCAGCCTTC AATAATGCAA ACGGCTCCTC TCTCTTGTCT TCAACAGAAT 
CAGCCATCTT CTGTTCAACA GTCAACACAA CCCATGCTTC AGCAACATCC ACAACCAGTC 
CTCAGGCAGC AGCAACAACC TCAACAGACT GCTGGTATTC ATCAGCAGCA GAGGTTGCTA 
GGCCAGCAGA ATAACCTTCC AAATCTGCAG CAGCAGCAAT TAATGGTTCA ACAAAATAAC 
CTATCAAGCA TGCATCAGCA ACAGCTGGGC TCTCAAAGTA ATGTCTCCAG TTTACAGCAG 
CAGCAGCTGC TTGGAGCTCA GTCTGGTAAC TCAAGCATGC AAACTAATCA GCACCCTGTA 
CACATGCTAC AACAGTCCAA GGTTACGCTG CAGCAACAAG CACAACAAAG TGCAGGTACT 
TTGTTACCTA ATCAAGGGCA GCAGTCACAA CCACAGCTGC CACAGCAGCA ATTGATGTCA 
CAGATCCAAT CACAGCCAGT GCAGCTGCAA CAGCAATCAA ATCCATTACA ACATGATCTG 
CAACAAAGAT TTCAAGCATC AGGTTCCTTG CTTCAACAGC AGAATGTGAC TGATCAGCAA 
AAGCAGTTAT ATCAATCTCA AAGAGCCCTT CCAGAAACGT CCCCAACATC CCTAGATTCT 
ACAACACAGA CTGGACATGT GAATGTCAAT GATTGGCAAG AGGAGATCTA CCAAAAGATC 
AAAGTCATGA AGGAGATGTA TTTCCCTGAA ATAAATGAAA TATACCAGAG AATTGCTGCC 
AAGTTACCGC AGCATGACTC TCATCCGCAA CAACCAAAGT CAGAACAGCT TGACAAGCTA 
AAGGCACTTA AGACCATGTT GGAGCGCCTG ATAATGTTCT TACAAGTTCC GAAAAACAAC 
ATCAAGCTTA ATTTCAAAGA GAAGTTGGGT TACTATGAGA ATCAGATTTT AAATTTTCTA 
AACACAAGCA GACCCAGAAA GCCTGTTCCT AATCTTCAGC AAGGACAGCT TCCCCAGCTT 
CATATGCAAC CTATGCAGCG ACCCCAATCT CAAGTTCCTC AATTGCAGTC CCATGAAAAT 
CAACTCAACC CCCAGTTGCA ATCAATGAAT TTGCAAGGTT CTGTACCAAC AATGCAGCAG 
AACAATGTGC CAAGCTTGCC GCATAATTCT CTTTCTTCTT TATCTGGGGT TTCTACATCA 
CAACCAAACA AGATGAATCC AATGCAATCA GCTTCCAATT TAGATTCTGG ACAAGGAAAT 
TCACTGAGCT CATTGCAGCA GGCACCTGTA GGATCTGTAC AACAGAATCC TGTGAGCAGT 
TCCCAACCTA CCAATTTTAA CACATTGTCA ACACAAAGTG GGGTGAGTAT GCTGCAGTCG 
AATATTCCTC TTCAGTTGAA TTCCAATATG ATTCAACAGC AGCATCTAAA ACAGCAGATG 
CTACAAACAC AACAGCTTAA ACAACAGTTC CAACAGCGTC AGCCGCAGCA GCAACAGCGT 
CAGACGCAGC AGCAACAGCA TTTAATGCAG AAGCAACAGA TGCTGCTGCA GCAGCAGCAG 
CAGCAGTTAC ACCAGCAAGC CAAGCAGCAG TTGCCTGCAC AGATGCAGAC ACAACAAATT 
CCACAGCCGC ATCAGATGAA TGATGTAAAT GAGATGAAGT TGAGACAGGG GATTGGAATC 
AAGCCAGCAG TCTTCCAACA ACATCTCTCG ACAGGTCAAC GCACTGCTTT CCCTCATCAA 
CAAATGAAAC CAGGATCCTC ATTTCCTATT TCTTCACCAC AAATGCTCCA GCATGCTTCC 
CCCCAATTAC AACAACATTC TTCTCCTCAG ATTGACCAGC AAAATCTACT GCCTTCTCTA 
ACTAAAACTG GAACACCTTT GCAATCTGCA AACTCGCCTT TTGTTGTCCC ATCTCCTTCA 
ACTCCTTTAG CTCCATCCCC TGTGCCAGGA GATTCTGAGA AACCCATTTC TGGTATCTCC 
TCAAATATTG TACACCAACC AACTGTTGCA CAAGCAACGG CTCCATCCCT TGCAATTGGC 
ACTCCTGGGA TATCTGCCTC GCCTTTGCTT GCAGAGTTTA CCAGTCCTGA TGGTGCTCAT 
GGTGGTGCTT TGACAACTGT TTCTGGCAAG TCGAATGTTA TGGAGCAACC ACTTGAACGC 
ATGATTAAAG CGGTGAAATC TCTTTCTCCT AAAGCACTTA GTGCATCTGT CAGTGACATT 
GGCTCTGTTG TCAGTATGAT TGACAGAATT GCTGGCTCGG CACCCGGTAA TGGGTCTAGA 
GCTGCAGTTG GTGAGGATCT GGTAGCGATG ACAAAATGTC GCCTTCAGGC AAGAAATTTC 
TTCACCCAAG ATGGGATGAC TGGATCGAAG AAAATGAGGC GCCATACAAG TGCCATGCCC 
TTGAATGTTG CATCATCAGC TGGCAGTGTA AGCGACAGTT TTAAGCAGTT TACTGGTCCA 
GAGACATCTG ATTTGGAGTC GACTGTAACG TCAAGTGTCA AGAGGCCTAA AATTGAGCTC 
TTCTTGCAGG CTAATCATGC CCTTTTGGAA GAGATAAGGG AAATAAATCA GCGACTTTTA 
GACACAGTGG TTGATATTAG TGATGAAGAT GTTGACTCAA CTGCAGCAGC AGCTGCTGCT 
GAAGGGGGTG GAGGAACCTT TGTCAAATGC TCATTTATCG CTGTGGCTCT GAGTCCAAAC 
TTGAAAGCAC AGTACACTTC AGCGCAAATG TCAACAATTC AGCCCCTTAG ATTGCTTGTG 
CCTACAAATT ATCCGAATTG CTCACCGATA CTGTTAGACA AGTTTCCATT TGAAGTCAGT 
AAAGAGTACG AGGATTTGTC AATAAAAGCT AAATTTAGGT TTAGCATCTC TCTTCGAAGC 
CTTTCACAGC CCATGTCGCT TGGAGACATA GCCAGGACTT GGGATGTCTG TGCCCATGCT 
GTTATTTCAG AGCATGCACA ACAAAGTGGC GGAGGCACCT TCAGCTCAAA ATATGGTTCT 
TGGGAAAACT GCGTGAGTGC AGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome of black cottonwood, Populus trichocarpa (Torr. & Gray).
Science (New York, N.Y.)313(5793)1596-604(2006 Sep)
Tuskan GA,Difazio S,Jansson S,Bohlmann J,Grigoriev I,Hellsten U,Putnam N,Ralph S,Rombauts S,Salamov A,Schein J,Sterck L,Aerts A,Bhalerao RR,Bhalerao RP,Blaudez D,Boerjan W,Brun A,Brunner A,Busov V,Campbell M,Carlson J,Chalot M,Chapman J,Chen GL,Cooper D,Coutinho PM,Couturier J,Covert S,Cronk Q,Cunningham R,Davis J,Degroeve S,D?jardin A,Depamphilis C,Detter J,Dirks B,Dubchak I,Duplessis S,Ehlting J,Ellis B,Gendler K,Goodstein D,Gribskov M,Grimwood J,Groover A,Gunter L,Hamberger B,Heinze B,Helariutta Y,Henrissat B,Holligan D,Holt R,Huang W,Islam-Faridi N,Jones S,Jones-Rhoades M,Jorgensen R,Joshi C,Kangasj?rvi J,Karlsson J,Kelleher C,Kirkpatrick R,Kirst M,Kohler A,Kalluri U,Larimer F,Leebens-Mack J,Lepl? JC,Locascio P,Lou Y,Lucas S,Martin F,Montanini B,Napoli C,Nelson DR,Nelson C,Nieminen K,Nilsson O,Pereda V,Peter G,Philippe R,Pilate G,Poliakov A,Razumovskaya J,Richardson P,Rinaldi C,Ritland K,Rouz? P,Ryaboy D,Schmutz J,Schrader J,Segerman B,Shin H,Siddiqui A,Sterky F,Terry A,Tsai CJ,Uberbacher E,Unneberg P,Vahala J,Wall K,Wessler S,Yang G,Yin T,Douglas C,Marra M,Sandberg G,Van de Peer Y,Rokhsar D

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POPTR_0001s21200g cDNA ORF clone, Populus trichocarpa(Populus balsamifera subsp. trichocarpa)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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