Nefm cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nefm
Entrez Gene ID	24588
Full Name	neurofilament medium
Synonyms	Nef3,Nfm
General protein information	Preferred Names neurofilament medium Names neurofilament medium polypeptide 160 kDa neurofilament protein NF-M Neurofilament protein, middle polypeptide neurofilament 3, medium neurofilament triplet M protein neurofilament, medium polypeptide
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 15 Map Location: 15p12
Summary	neuronal intermediate filament protein; important component of the cytoskeleton [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_017029.2	NP_058725.1	neurofilament medium polypeptide

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Nefm	NP_032717.2
Rattus norvegicus (Norway rat)	Nefm	NP_058725.1

Gene Ontology
Bibliography

Related articles in PubMed

Neurofilament subunits are integral components of synapses and modulate neurotransmission and behavior in vivo.
Yuan A, Sershen H, Veeranna, Basavarajappa BS, Kumar A, Hashim A, Berg M, Lee JH, Sato Y, Rao MV, Mohan PS, Dyakin V, Julien JP, Lee VM, Nixon RA
Molecular psychiatry20(8)986-94(2015 Aug)

Loss of Usp9x disrupts cortical architecture, hippocampal development and TGF?-mediated axonogenesis.
Stegeman S, Jolly LA, Premarathne S, Gecz J, Richards LJ, Mackay-Sim A, Wood SA
PloS one8(7)e68287(2013)

Genesis of muscle fiber-type diversity during mouse embryogenesis relies on Six1 and Six4 gene expression.
Richard AF, Demignon J, Sakakibara I, Pujol J, Favier M, Strochlic L, Le Grand F, Sgarioto N, Guernec A, Schmitt A, Cagnard N, Huang R, Legay C, Guillet-Deniau I, Maire P
Developmental biology359(2)303-20(2011 Nov)

Direct evidence of phosphorylated neuronal intermediate filament proteins in neurofibrillary tangles (NFTs): phosphoproteomics of Alzheimer's NFTs.
Rudrabhatla P, Jaffe H, Pant HC
FASEB journal : official publication of the Federation of American Societies for Experimental Biology25(11)3896-905(2011 Nov)

Induced loss of ADAR2 engenders slow death of motor neurons from Q/R site-unedited GluR2.
Hideyama T, Yamashita T, Suzuki T, Tsuji S, Higuchi M, Seeburg PH, Takahashi R, Misawa H, Kwak S
The Journal of neuroscience : the official journal of the Society for Neuroscience30(36)11917-25(2010 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

head domain phosphorylation of endogenous NF-M by forskolin-activated PKA inhibits NF-M tail domain phosphorylation in rat cortical neurons
Title: Phosphorylation of the head domain of neurofilament protein (NF-M): a factor regulating topographic phosphorylation of NF-M tail domain KSP sites in neurons.

The following Nefm gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nefm cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa00009	NM_017029.2 Latest version!	Rattus norvegicus neurofilament medium (Nefm), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nefm ( NM_017029.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058725.1 Rattus norvegicus neurofilament medium (Nefm), mRNA.

Price & Availability↑

CloneID	ORa00009
Clone ID Related Accession (Same CDS sequence)	NM_017029.2
Accession Version	NM_017029.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2538bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	neurofilament medium polypeptide
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from Z12152.1. On Aug 31, 2012 this sequence version replaced NM_017029.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Z12152.1, M18628.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGAGCTACA CGCTGGACTC GCTGGGCAAC CCGTCCGCCT ACCGGCGCGT CACCGAGACC 
CCGTCCAGCT TCAGTCGTGT GAGCGGTTCC CCGTCCAGCG GCTTCCGCTC GCAGTCCTGG 
TCCCGCGGCT CGCCCAGCAC CGTGTCCTCC TCCTACAAGC GCAGCGCGCT CGCCCCGCGC 
CTCGCCTACA GCTCGGCTAT GCTCAGCTCG GCCGAGAGCA GCCTCGACTT CAGCCAGTCC 
TCTTCGCTGC TTAACGGCGG CTCCGGCGGC GACTACAAGC TGTCCCGCTC AAACGAGAAA 
GAGCAGCTGC AGGGGCTGAA CGACCGTTTC GCCGGCTACA TCGAGAAAGT GCACTACTTG 
GAACAACAGA ACAAGGAGAT CGAGGCAGAG ATCCACGCGC TGCGGCAGAA GCAGGCCTCG 
CACGCCCAGC TGGGTGACGC TTACGACCAG GAGATCCGAG AGCTGCGCGC CACCCTGGAG 
ATGGTGAATC ACGAGAAGGC TCAAGTGCAG CTGGACTCTG ATCACTTGGA GGAAGACATC 
CACCGGCTCA AGGAGCGCTT CGAGGAGGAG GCGCGGCTGC GGGACGACAC CGAGGCTGCC 
ATCCGGGCGC TGCGCAAAGA CATAGAGGAG TCGTCGATGG TTAAGGTGGA GCTGGACAAG 
AAGGTGCAGT CGCTGCAGGA TGAGGTGGCC TTCCTGCGGA GCAATCACGA AGAGGAGGTG 
GCCGACCTGC TGGCCCAGAT CCAGGCGTCG CACATCACCG TAGAGCGCAA AGACTACCTG 
AAGACAGACA TCTCCACGGC GCTGAAAGAG ATCCGCTCCC AGCTCGAGTG TCACTCCGAC 
CAGAACATGC ACCAGGCCGA AGAGTGGTTC AAATGCCGCT ACGCCAAGCT CACCGAGGCG 
GCCGAGCAGA ACAAGGAGGC CATCCGCTCC GCTAAAGAAG AGATCGCCGA GTACCGGCGC 
CAGCTGCAGT CCAAGAGCAT TGAGCTCGAG TCGGTGCGAG GCACTAAGGA GTCCCTGGAA 
CGGCAGCTCA GCGACATCGA GGAGCGCCAC AACCACGACC TCAGCAGCTA CCAGGACACC 
ATCCAGCAGC TGGAAAATGA GCTTCGGGGA ACAAAGTGGG AAATGGCTCG TCATTTGCGA 
GAATACCAGG ATCTCCTTAA CGTCAAGATG GCTCTGGACA TCGAGATCGC CGCATATAGG 
AAACTACTGG AGGGTGAAGA GACCAGATTT AGCACATTTT CAGGAAGCAT CACTGGGCCT 
CTGTACACAC ACCGACAGCC CTCAGTCACA ATATCCAGTA AGATTCAGAA GACCAAAGTC 
GAGGCCCCCA AGCTCAAGGT CCAACACAAA TTTGTGGAGG AGATCATTGA GGAGACTAAA 
GTGGAAGATG AGAAGTCAGA AATGGAAGAC GCCCTCACAG TCATTGCAGA GGAATTGGCA 
GCCTCTGCCA AAGAGGAGAA AGAAGAGGCA GAAGAAAAGG AAGAGGAACC GGAAGTTGAA 
AAGTCTCCCG TGAAGTCTCC TGAGGCTAAG GAAGAGGAGG AAGGGGAAAA GGAGGAAGAA 
GAGGAAGGCC AAGAGGAAGA AGAGGAGGAA GATGAAGGTG TCAAGTCAGA CCAGGCAGAA 
GAGGGAGGAT CTGAGAAGGA AGGCTCGAGT GAAAAGGATG AAGGTGAGCA AGAAGAAGAA 
GGGGAAACTG AGGCAGAAGG TGAAGGAGAG GAAGCAGAAG CTAAGGAGGA AAAGAAAACA 
GAGGGAAAGG TCGAGGAAAT GGCTATCAAG GAGGAAATCA AGGTCGAGAA GCCCGAGAAA 
GCCAAGTCCC CTGTGCCAAA ATCACCCGTG GAAGAAGTAA AGCCAAAACC AGAAGCCAAA 
GCCGGAAAGG ATGAGCAGAA GGAGGAAGAG AAAGTTGAGG AGAAGAAGGA GGTAGCCAAG 
GAATCACCCA AGGAAGAGAA GGTGGAGAAA AAGGAGGAGA AGCCAAAAGA TGTCCCAGAT 
AAAAAGAAGG CTGAGTCCCC AGTGAAAGAA AAGGCCGTAG AGGAAATGAT CACCATTACT 
AAGTCGGTAA AGGTGAGCCT GGAGAAAGAC ACCAAAGAGG AGAAGCCTCA GCAGCAGGAG 
AAGGTGAAGG AGAAGGCAGA GGAGGAGGGG GGTAGTGAGG AGGAAGTGGG TGACAAAAGC 
CCGCAAGAAT CCAAGAAGGA AGACATAGCT ATCAATGGGG AGGTGGAAGG AAAAGAGGAG 
GAGGAGCAGG AAACTCAGGA GAAGGGCAGT GGGCAAGAGG AGGAGAAAGG GGTGGTCACT 
AATGGCTTAG ATGTGAGCCC TGCGGAGGAA AAGAAAGGGG AGGATAGAAG TGATGACAAA 
GTGGTGGTGA CCAAGAAGGT AGAAAAAATC ACCAGCGAGG GAGGCGATGG TGCTACCAAA 
TACATCACCA AATCTGTTAC TGTCACTCAA AAGGTTGAAG AGCATGAGGA GACCTTTGAG 
GAGAAGCTGG TGTCAACTAA AAAGGTAGAA AAGGTCACTT CACATGCCAT AGTCAAGGAA 
GTCACCCAGG GTGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058725.1
CDS	36..2573
Translation	MSYTLDSLGNPSAYRRVTETPSSFSRVSGSPSSGFRSQSWSRGSPSTVSSSYKRSALAPRLAYSSAMLSSAESSLDFSQSSSLLNGGSGGDYKLSRSNEKEQLQGLNDRFAGYIEKVHYLEQQNKEIEAEIHALRQKQASHAQLGDAYDQEIRELRATLEMVNHEKAQVQLDSDHLEEDIHRLKERFEEEARLRDDTEAAIRALRKDIEESSMVKVELDKKVQSLQDEVAFLRSNHEEEVADLLAQIQASHITVERKDYLKTDISTALKEIRSQLECHSDQNMHQAEEWFKCRYAKLTEAAEQNKEAIRSAKEEIAEYRRQLQSKSIELESVRGTKESLERQLSDIEERHNHDLSSYQDTIQQLENELRGTKWEMARHLREYQDLLNVKMALDIEIAAYRKLLEGEETRFSTFSGSITGPLYTHRQPSVTISSKIQKTKVEAPKLKVQHKFVEEIIEETKVEDEKSEMEDALTVIAEELAASAKEEKEEAEEKEEEPEVEKSPVKSPEAKEEEEGEKEEEEEGQEEEEEEDEGVKSDQAEEGGSEKEGSSEKDEGEQEEEGETEAEGEGEEAEAKEEKKTEGKVEEMAIKEEIKVEKPEKAKSPVPKSPVEEVKPKPEAKAGKDEQKEEEKVEEKKEVAKESPKEEKVEKKEEKPKDVPDKKKAESPVKEKAVEEMITITKSVKVSLEKDTKEEKPQQQEKVKEKAEEEGGSEEEVGDKSPQESKKEDIAINGEVEGKEEEEQETQEKGSGQEEEKGVVTNGLDVSPAEEKKGEDRSDDKVVVTKKVEKITSEGGDGATKYITKSVTVTQKVEEHEETFEEKLVSTKKVEKVTSHAIVKEVTQGD

Target ORF information:

RefSeq Version	NM_017029.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neurofilament medium (Nefm), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017029.2

ORF Insert Sequence:

ATGAGCTACA CGCTGGACTC GCTGGGCAAC CCGTCCGCCT ACCGGCGCGT CACCGAGACC 
CCGTCCAGCT TCAGTCGTGT GAGCGGTTCC CCGTCCAGCG GCTTCCGCTC GCAGTCCTGG 
TCCCGCGGCT CGCCCAGCAC CGTGTCCTCC TCCTACAAGC GCAGCGCGCT CGCCCCGCGC 
CTCGCCTACA GCTCGGCTAT GCTCAGCTCG GCCGAGAGCA GCCTCGACTT CAGCCAGTCC 
TCTTCGCTGC TTAACGGCGG CTCCGGCGGC GACTACAAGC TGTCCCGCTC AAACGAGAAA 
GAGCAGCTGC AGGGGCTGAA CGACCGTTTC GCCGGCTACA TCGAGAAAGT GCACTACTTG 
GAACAACAGA ACAAGGAGAT CGAGGCAGAG ATCCACGCGC TGCGGCAGAA GCAGGCCTCG 
CACGCCCAGC TGGGTGACGC TTACGACCAG GAGATCCGAG AGCTGCGCGC CACCCTGGAG 
ATGGTGAATC ACGAGAAGGC TCAAGTGCAG CTGGACTCTG ATCACTTGGA GGAAGACATC 
CACCGGCTCA AGGAGCGCTT CGAGGAGGAG GCGCGGCTGC GGGACGACAC CGAGGCTGCC 
ATCCGGGCGC TGCGCAAAGA CATAGAGGAG TCGTCGATGG TTAAGGTGGA GCTGGACAAG 
AAGGTGCAGT CGCTGCAGGA TGAGGTGGCC TTCCTGCGGA GCAATCACGA AGAGGAGGTG 
GCCGACCTGC TGGCCCAGAT CCAGGCGTCG CACATCACCG TAGAGCGCAA AGACTACCTG 
AAGACAGACA TCTCCACGGC GCTGAAAGAG ATCCGCTCCC AGCTCGAGTG TCACTCCGAC 
CAGAACATGC ACCAGGCCGA AGAGTGGTTC AAATGCCGCT ACGCCAAGCT CACCGAGGCG 
GCCGAGCAGA ACAAGGAGGC CATCCGCTCC GCTAAAGAAG AGATCGCCGA GTACCGGCGC 
CAGCTGCAGT CCAAGAGCAT TGAGCTCGAG TCGGTGCGAG GCACTAAGGA GTCCCTGGAA 
CGGCAGCTCA GCGACATCGA GGAGCGCCAC AACCACGACC TCAGCAGCTA CCAGGACACC 
ATCCAGCAGC TGGAAAATGA GCTTCGGGGA ACAAAGTGGG AAATGGCTCG TCATTTGCGA 
GAATACCAGG ATCTCCTTAA CGTCAAGATG GCTCTGGACA TCGAGATCGC CGCATATAGG 
AAACTACTGG AGGGTGAAGA GACCAGATTT AGCACATTTT CAGGAAGCAT CACTGGGCCT 
CTGTACACAC ACCGACAGCC CTCAGTCACA ATATCCAGTA AGATTCAGAA GACCAAAGTC 
GAGGCCCCCA AGCTCAAGGT CCAACACAAA TTTGTGGAGG AGATCATTGA GGAGACTAAA 
GTGGAAGATG AGAAGTCAGA AATGGAAGAC GCCCTCACAG TCATTGCAGA GGAATTGGCA 
GCCTCTGCCA AAGAGGAGAA AGAAGAGGCA GAAGAAAAGG AAGAGGAACC GGAAGTTGAA 
AAGTCTCCCG TGAAGTCTCC TGAGGCTAAG GAAGAGGAGG AAGGGGAAAA GGAGGAAGAA 
GAGGAAGGCC AAGAGGAAGA AGAGGAGGAA GATGAAGGTG TCAAGTCAGA CCAGGCAGAA 
GAGGGAGGAT CTGAGAAGGA AGGCTCGAGT GAAAAGGATG AAGGTGAGCA AGAAGAAGAA 
GGGGAAACTG AGGCAGAAGG TGAAGGAGAG GAAGCAGAAG CTAAGGAGGA AAAGAAAACA 
GAGGGAAAGG TCGAGGAAAT GGCTATCAAG GAGGAAATCA AGGTCGAGAA GCCCGAGAAA 
GCCAAGTCCC CTGTGCCAAA ATCACCCGTG GAAGAAGTAA AGCCAAAACC AGAAGCCAAA 
GCCGGAAAGG ATGAGCAGAA GGAGGAAGAG AAAGTTGAGG AGAAGAAGGA GGTAGCCAAG 
GAATCACCCA AGGAAGAGAA GGTGGAGAAA AAGGAGGAGA AGCCAAAAGA TGTCCCAGAT 
AAAAAGAAGG CTGAGTCCCC AGTGAAAGAA AAGGCCGTAG AGGAAATGAT CACCATTACT 
AAGTCGGTAA AGGTGAGCCT GGAGAAAGAC ACCAAAGAGG AGAAGCCTCA GCAGCAGGAG 
AAGGTGAAGG AGAAGGCAGA GGAGGAGGGG GGTAGTGAGG AGGAAGTGGG TGACAAAAGC 
CCGCAAGAAT CCAAGAAGGA AGACATAGCT ATCAATGGGG AGGTGGAAGG AAAAGAGGAG 
GAGGAGCAGG AAACTCAGGA GAAGGGCAGT GGGCAAGAGG AGGAGAAAGG GGTGGTCACT 
AATGGCTTAG ATGTGAGCCC TGCGGAGGAA AAGAAAGGGG AGGATAGAAG TGATGACAAA 
GTGGTGGTGA CCAAGAAGGT AGAAAAAATC ACCAGCGAGG GAGGCGATGG TGCTACCAAA 
TACATCACCA AATCTGTTAC TGTCACTCAA AAGGTTGAAG AGCATGAGGA GACCTTTGAG 
GAGAAGCTGG TGTCAACTAA AAAGGTAGAA AAGGTCACTT CACATGCCAT AGTCAAGGAA 
GTCACCCAGG GTGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Neurofilament subunits are integral components of synapses and modulate neurotransmission and behavior in vivo.
Molecular psychiatry20(8)986-94(2015 Aug)
Yuan A,Sershen H,Veeranna,Basavarajappa BS,Kumar A,Hashim A,Berg M,Lee JH,Sato Y,Rao MV,Mohan PS,Dyakin V,Julien JP,Lee VM,Nixon RA

Loss of Usp9x disrupts cortical architecture, hippocampal development and TGF?-mediated axonogenesis.
PloS one8(7)e68287(2013)
Stegeman S,Jolly LA,Premarathne S,Gecz J,Richards LJ,Mackay-Sim A,Wood SA

Genesis of muscle fiber-type diversity during mouse embryogenesis relies on Six1 and Six4 gene expression.
Developmental biology359(2)303-20(2011 Nov)
Richard AF,Demignon J,Sakakibara I,Pujol J,Favier M,Strochlic L,Le Grand F,Sgarioto N,Guernec A,Schmitt A,Cagnard N,Huang R,Legay C,Guillet-Deniau I,Maire P

Direct evidence of phosphorylated neuronal intermediate filament proteins in neurofibrillary tangles (NFTs): phosphoproteomics of Alzheimer's NFTs.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology25(11)3896-905(2011 Nov)
Rudrabhatla P,Jaffe H,Pant HC

Induced loss of ADAR2 engenders slow death of motor neurons from Q/R site-unedited GluR2.
The Journal of neuroscience : the official journal of the Society for Neuroscience30(36)11917-25(2010 Sep)
Hideyama T,Yamashita T,Suzuki T,Tsuji S,Higuchi M,Seeburg PH,Takahashi R,Misawa H,Kwak S

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Nefm cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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