Apoa4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Apoa4
Entrez Gene ID	25080
Full Name	apolipoprotein A4
Synonyms	Apo-AIV,ApoA-IV,apoAIV
General protein information	Preferred Names apolipoprotein A4 Names apolipoprotein A-IV
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q22
Summary	may play a role in lipid transport [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_012737.1	NP_036869.1	apolipoprotein A-IV precursor

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	zgc:194131	NP_001122230.1
Danio rerio (zebrafish)	LOC100005908	XP_001344822.2
Pan troglodytes (chimpanzee)	APOA4	XP_522192.2
	APOA4	XP_001090730.2
Canis lupus familiaris (dog)	APOA4	XP_546510.3
Bos taurus (cattle)	APOA4	NP_001032557.1
Danio rerio (zebrafish)	zgc:112992	NP_001017909.1
Danio rerio (zebrafish)	apoa4	NP_001073330.1
Danio rerio (zebrafish)	si:dkey-7f3.15	XP_001338037.1
Xenopus tropicalis (tropical clawed frog)	apoa4	NP_001008099.1
Rattus norvegicus (Norway rat)	Apoa4	NP_036869.1
Gallus gallus (chicken)	APOA4	NP_990269.1
Homo sapiens (human)	APOA4	NP_000473.2
Mus musculus (house mouse)	Apoa4	NP_031494.2

Gene Ontology
Bibliography

Related articles in PubMed

Apolipoprotein A-IV exerts its anorectic action through a PI3K/Akt signaling pathway in the hypothalamus.
Shen L, Lo CC, Woollett LA, Liu M
Biochemical and biophysical research communications494(1-2)152-157(2017 12)

Glycoproteomics Reveals Decorin Peptides With Anti-Myostatin Activity in Human Atrial Fibrillation.
Barallobre-Barreiro J, Gupta SK, Zoccarato A, Kitazume-Taneike R, Fava M, Yin X, Werner T, Hirt MN, Zampetaki A, Viviano A, Chong M, Bern M, Kourliouros A, Domenech N, Willeit P, Shah AM, Jahangiri M, Schaefer L, Fischer JW, Iozzo RV, Viner R, Thum T, Heineke J, Kichler A, Otsu K, Mayr M
Circulation134(11)817-32(2016 Sep)

Extracellular matrix remodelling in response to venous hypertension: proteomics of human varicose veins.
Barallobre-Barreiro J, Oklu R, Lynch M, Fava M, Baig F, Yin X, Barwari T, Potier DN, Albadawi H, Jahangiri M, Porter KE, Watkins MT, Misra S, Stoughton J, Mayr M
Cardiovascular research110(3)419-30(2016 06)

Massive bowel resection upregulates the intestinal mRNA expression levels of cellular retinol-binding protein II and apolipoprotein A-IV and alters the intestinal vitamin A status in rats.
Hebiguchi T, Mezaki Y, Morii M, Watanabe R, Yoshikawa K, Miura M, Imai K, Senoo H, Yoshino H
International journal of molecular medicine35(3)724-30(2015 Mar)

Estradiol stimulates apolipoprotein A-IV gene expression in the nucleus of the solitary tract through estrogen receptor-?.
Shen L, Liu Y, Wang DQ, Tso P, Woods SC, Liu M
Endocrinology155(10)3882-90(2014 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

demonstrate a critical role of the PI3K/Akt pathway in apoA-IV's anorectic action in lean rats and suggest a defective PI3K pathway in the ventromedial hypothalamus is responsible for the impaired apoA-IV's anorectic action in the diet-induced obese animals
Title: Apolipoprotein A-IV exerts its anorectic action through a PI3K/Akt signaling pathway in the hypothalamus.

These results suggest that the elevated mRNA expression levels of Rbp2 and Apoa4 in the rats with SB contribute to the effective esterification and transport of vitamin A.
Title: Massive bowel resection upregulates the intestinal mRNA expression levels of cellular retinol-binding protein II and apolipoprotein A-IV and alters the intestinal vitamin A status in rats.

Nuclear ERalpha is the primary mediator of E2's action on apo A-IV gene expression.
Title: Estradiol stimulates apolipoprotein A-IV gene expression in the nucleus of the solitary tract through estrogen receptor-?.

Data suggest that apoAIV in NTS (nucleus of the solitary tract) or/and peripheral Cck (cholecystokinin) require vagal Cck1r (cholecystokinin receptor 1) signaling to elicit satiation; high-fat diet reduces satiating capacity of these signals.
Title: Intraperitoneal CCK and fourth-intraventricular Apo AIV require both peripheral and NTS CCK1R to reduce food intake in male rats.

Octreotide intervention down-regulated expression of apoB mRNA and apoB48 protein, as well as MTP mRNA and proteins. ApoAIV mRNA was not significantly different between obese and octreotide-treated rats.
Title: Octreotide promotes weight loss via suppression of intestinal MTP and apoB48 expression in diet-induced obesity rats.

The following Apoa4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Apoa4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12894	NM_012737.1 Latest version!	Rattus norvegicus apolipoprotein A4 (Apoa4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Apoa4 ( NM_012737.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036869.1 Rattus norvegicus apolipoprotein A4 (Apoa4), mRNA.

Price & Availability↑

CloneID	ORa12894
Clone ID Related Accession (Same CDS sequence)	NM_012737.1
Accession Version	NM_012737.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1176bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Rattus norvegicus(Norway rat)
Product	apolipoprotein A-IV precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M00002.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M00002.1, FQ218620.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689598, SAMEA2689605 [ECO:0000348] ##Evidence-Data-END##

ATGTTCCTGA AGGCTGTGGT GCTGACCGTG GCCCTGGTGG CCATCACCGG GACCCAGGCT 
GAGGTCACTT CCGACCAGGT GGCCAATGTG ATGTGGGACT ACTTCACCCA GCTAAGCAAC 
AATGCCAAGG AGGCTGTGGA ACAACTGCAG AAGACAGATG TCACTCAACA GCTCAATACC 
CTCTTCCAGG ACAAACTTGG GAACATTAAC ACCTATGCCG ATGACCTGCA GAACAAGCTG 
GTGCCCTTCG CCGTTCAACT GAGTGGACAT CTAACCAAGG AAACAGAGAG GGTGAGGGAA 
GAGATCCAGA AGGAGCTCGA GGACCTACGG GCCAACATGA TGCCCCATGC CAACAAAGTG 
AGCCAGATGT TCGGGGACAA CGTGCAGAAG TTGCAGGAAC ACCTGAGGCC CTATGCCACG 
GACCTGCAAG CTCAGATCAA CGCACAGACC CAGGATATGA AACGCCAGCT GACCCCCTAC 
ATCCAGCGCA TGCAGACCAC AATACAAGAC AATGTGGAAA ACCTGCAGTC CTCCATGGTG 
CCCTTTGCCA ACGAGCTAAA GGAAAAGTTT AACCAGAATA TGGAAGGGCT CAAGGGGCAA 
CTAACCCCCC GTGCCAACGA GCTGAAGGCC ACGATCGACC AGAACCTGGA AGACCTGCGC 
AGCAGACTGG CCCCTCTCGC GGAGGGTGTG CAGGAAAAAC TCAACCATCA GATGGAAGGC 
TTGGCCTTCC AGATGAAGAA GAACGCTGAG GAGCTCCATA CCAAAGTCTC CACAAATATC 
GACCAGCTGC AGAAGAATCT GGCCCCGCTG GTGGAAGATG TGCAAAGCAA GCTGAAAGGC 
AACACGGAAG GACTGCAGAA GTCTCTGGAA GACCTGAACA AGCAGCTGGA CCAGCAGGTG 
GAGGTGTTCC GGCGTGCTGT GGAGCCCCTG GGGGATAAGT TCAACATGGC TCTGGTGCAG 
CAGATGGAGA AGTTCAGGCA GCAGCTGGGC TCCGATTCGG GGGACGTGGA AAGCCACTTG 
AGCTTCCTGG AGAAGAACCT GAGGGAAAAG GTCAGCTCCT TTATGAGCAC CCTGCAAAAA 
AAGGGGAGCC CAGACCAGCC CCTAGCCCTC CCCCTCCCGG AGCAGGTTCA GGAACAGGTC 
CAGGAGCAGG TGCAGCCCAA ACCTCTGGAG AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036869.1
CDS	92..1267
Translation	MFLKAVVLTVALVAITGTQAEVTSDQVANVMWDYFTQLSNNAKEAVEQLQKTDVTQQLNTLFQDKLGNINTYADDLQNKLVPFAVQLSGHLTKETERVREEIQKELEDLRANMMPHANKVSQMFGDNVQKLQEHLRPYATDLQAQINAQTQDMKRQLTPYIQRMQTTIQDNVENLQSSMVPFANELKEKFNQNMEGLKGQLTPRANELKATIDQNLEDLRSRLAPLAEGVQEKLNHQMEGLAFQMKKNAEELHTKVSTNIDQLQKNLAPLVEDVQSKLKGNTEGLQKSLEDLNKQLDQQVEVFRRAVEPLGDKFNMALVQQMEKFRQQLGSDSGDVESHLSFLEKNLREKVSSFMSTLQKKGSPDQPLALPLPEQVQEQVQEQVQPKPLES

Target ORF information:

RefSeq Version	NM_012737.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus apolipoprotein A4 (Apoa4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012737.1

ORF Insert Sequence:

ATGTTCCTGA AGGCTGTGGT GCTGACCGTG GCCCTGGTGG CCATCACCGG GACCCAGGCT 
GAGGTCACTT CCGACCAGGT GGCCAATGTG ATGTGGGACT ACTTCACCCA GCTAAGCAAC 
AATGCCAAGG AGGCTGTGGA ACAACTGCAG AAGACAGATG TCACTCAACA GCTCAATACC 
CTCTTCCAGG ACAAACTTGG GAACATTAAC ACCTATGCCG ATGACCTGCA GAACAAGCTG 
GTGCCCTTCG CCGTTCAACT GAGTGGACAT CTAACCAAGG AAACAGAGAG GGTGAGGGAA 
GAGATCCAGA AGGAGCTCGA GGACCTACGG GCCAACATGA TGCCCCATGC CAACAAAGTG 
AGCCAGATGT TCGGGGACAA CGTGCAGAAG TTGCAGGAAC ACCTGAGGCC CTATGCCACG 
GACCTGCAAG CTCAGATCAA CGCACAGACC CAGGATATGA AACGCCAGCT GACCCCCTAC 
ATCCAGCGCA TGCAGACCAC AATACAAGAC AATGTGGAAA ACCTGCAGTC CTCCATGGTG 
CCCTTTGCCA ACGAGCTAAA GGAAAAGTTT AACCAGAATA TGGAAGGGCT CAAGGGGCAA 
CTAACCCCCC GTGCCAACGA GCTGAAGGCC ACGATCGACC AGAACCTGGA AGACCTGCGC 
AGCAGACTGG CCCCTCTCGC GGAGGGTGTG CAGGAAAAAC TCAACCATCA GATGGAAGGC 
TTGGCCTTCC AGATGAAGAA GAACGCTGAG GAGCTCCATA CCAAAGTCTC CACAAATATC 
GACCAGCTGC AGAAGAATCT GGCCCCGCTG GTGGAAGATG TGCAAAGCAA GCTGAAAGGC 
AACACGGAAG GACTGCAGAA GTCTCTGGAA GACCTGAACA AGCAGCTGGA CCAGCAGGTG 
GAGGTGTTCC GGCGTGCTGT GGAGCCCCTG GGGGATAAGT TCAACATGGC TCTGGTGCAG 
CAGATGGAGA AGTTCAGGCA GCAGCTGGGC TCCGATTCGG GGGACGTGGA AAGCCACTTG 
AGCTTCCTGG AGAAGAACCT GAGGGAAAAG GTCAGCTCCT TTATGAGCAC CCTGCAAAAA 
AAGGGGAGCC CAGACCAGCC CCTAGCCCTC CCCCTCCCGG AGCAGGTTCA GGAACAGGTC 
CAGGAGCAGG TGCAGCCCAA ACCTCTGGAG AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Apolipoprotein A-IV exerts its anorectic action through a PI3K/Akt signaling pathway in the hypothalamus.
Biochemical and biophysical research communications494(1-2)152-157(2017 12)
Shen L,Lo CC,Woollett LA,Liu M

Glycoproteomics Reveals Decorin Peptides With Anti-Myostatin Activity in Human Atrial Fibrillation.
Circulation134(11)817-32(2016 Sep)
Barallobre-Barreiro J,Gupta SK,Zoccarato A,Kitazume-Taneike R,Fava M,Yin X,Werner T,Hirt MN,Zampetaki A,Viviano A,Chong M,Bern M,Kourliouros A,Domenech N,Willeit P,Shah AM,Jahangiri M,Schaefer L,Fischer JW,Iozzo RV,Viner R,Thum T,Heineke J,Kichler A,Otsu K,Mayr M

Extracellular matrix remodelling in response to venous hypertension: proteomics of human varicose veins.
Cardiovascular research110(3)419-30(2016 06)
Barallobre-Barreiro J,Oklu R,Lynch M,Fava M,Baig F,Yin X,Barwari T,Potier DN,Albadawi H,Jahangiri M,Porter KE,Watkins MT,Misra S,Stoughton J,Mayr M

Massive bowel resection upregulates the intestinal mRNA expression levels of cellular retinol-binding protein II and apolipoprotein A-IV and alters the intestinal vitamin A status in rats.
International journal of molecular medicine35(3)724-30(2015 Mar)
Hebiguchi T,Mezaki Y,Morii M,Watanabe R,Yoshikawa K,Miura M,Imai K,Senoo H,Yoshino H

Estradiol stimulates apolipoprotein A-IV gene expression in the nucleus of the solitary tract through estrogen receptor-?.
Endocrinology155(10)3882-90(2014 Oct)
Shen L,Liu Y,Wang DQ,Tso P,Woods SC,Liu M