Gtf2a2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gtf2a2
Entrez Gene ID	83828
Full Name	general transcription factor IIA, 2
General protein information	Preferred Names general transcription factor IIA, 2 Names transcription initiation factor IIA subunit 2 TFIIA-gamma general transcription factor IIA subunit 2 general transcription factor IIa, 2 (12kD subunit) transcription initiation factor IIA gamma chain
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q24
Summary	mouse homolog is a subunit of one of the general transcription factors that, with RNA polymerase II holoenzyme, forms the preinitiation complex to initiate transcription of class II genes [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006243380.3	XP_006243442.1	transcription initiation factor IIA subunit 2 isoform X1
NM_053345.1	NP_445797.1	transcription initiation factor IIA subunit 2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	GTF2A2	NP_001032708.1
Mus musculus (house mouse)	Gtf2a2	NP_001034608.1
Drosophila melanogaster (fruit fly)	TfIIA-S	NP_524467.1
Eremothecium gossypii	AGOS_ABR071W	NP_983017.2
Oryza sativa (rice)	Os05g0107700	NP_001054426.1
	GTF2A2	XP_001100320.1
Canis lupus familiaris (dog)	GTF2A2	XP_005638528.1
Gallus gallus (chicken)	GTF2A2	NP_001075177.1
Saccharomyces cerevisiae (baker's yeast)	TOA2	NP_012865.1
Oryza sativa (rice)	Os01g0970500	NP_001045526.1
Kluyveromyces lactis	KLLA0C12815g	XP_452771.1
Magnaporthe oryzae (rice blast fungus)	MGG_06107	XP_003711973.1
Neurospora crassa	NCU09748	XP_964450.1
Xenopus tropicalis (tropical clawed frog)	gtf2a2	NP_001005107.1
Homo sapiens (human)	GTF2A2	NP_004483.1
Pan troglodytes (chimpanzee)	GTF2A2	XP_001173111.1
Rattus norvegicus (Norway rat)	Gtf2a2	NP_445797.1
Danio rerio (zebrafish)	gtf2a2	NP_001018441.1
Caenorhabditis elegans (roundworm)	CELE_Y111B2A.13	NP_499644.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004370	XP_313653.4
Schizosaccharomyces pombe (fission yeast)	SPCC553.11c	NP_587763.2
Arabidopsis thaliana (thale cress)	AT4G24440	NP_194175.1

Gene Ontology
Bibliography

Related articles in PubMed

Near-atomic resolution visualization of human transcription promoter opening.
He Y, Yan C, Fang J, Inouye C, Tjian R, Ivanov I, Nogales E
Nature533(7603)359-65(2016 05)

Structural insights into transcription initiation by RNA polymerase II.
Gr?nberg S, Hahn S
Trends in biochemical sciences38(12)603-11(2013 Dec)

The homeobox gene Mohawk represses transcription by recruiting the sin3A/HDAC co-repressor complex.
Anderson DM, Beres BJ, Wilson-Rawls J, Rawls A
Developmental dynamics : an official publication of the American Association of Anatomists238(3)572-80(2009 Mar)

General transcription factor IIA-gamma increases osteoblast-specific osteocalcin gene expression via activating transcription factor 4 and runt-related transcription factor 2.
Yu S, Jiang Y, Galson DL, Luo M, Lai Y, Lu Y, Ouyang HJ, Zhang J, Xiao G
The Journal of biological chemistry283(9)5542-53(2008 Feb)

TFIIAalpha/beta-like factor is encoded by a germ cell-specific gene whose expression is up-regulated with other general transcription factors during spermatogenesis in the mouse.
Han SY, Zhou L, Upadhyaya A, Lee SH, Parker KL, DeJong J
Biology of reproduction64(2)507-17(2001 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Gtf2a2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gtf2a2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa09322	NM_053345.1 Latest version!	Rattus norvegicus general transcription factor 2A subunit 2 (Gtf2a2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa09322	XM_006243380.3 Latest version!	Rattus norvegicus general transcription factor IIA, 2 (Gtf2a2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gtf2a2 ( NM_053345.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_445797.1 Rattus norvegicus general transcription factor 2A subunit 2 (Gtf2a2), mRNA.

Price & Availability↑

CloneID	ORa09322
Clone ID Related Accession (Same CDS sequence)	XM_006243380.3 , NM_053345.1
Accession Version	NM_053345.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 330bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Rattus norvegicus(Norway rat)
Product	transcription initiation factor IIA subunit 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF000944.1. ##Evidence-Data-START## Transcript exon combination :: AF000944.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCATATC AGTTATACAG AAATACAACT TTGGGGAACA GTCTTCAAGA GAGCCTTGAT 
GAGCTCATAC AGTCTCAACA GATCACCCCC CAGCTTGCCC TTCAAGTTCT ACTTCAGTTT 
GATAAAGCTA TAAATTCAGC ATTGGCTCAG AGAGTCAGGA ACAGAGTCAA TTTCAGGGGC 
TCTCTAAATA CATACAGATT CTGCGATAAT GTTTGGACTT TTGTATTGAA TGATGTTGAA 
TTCAGAGAGG TGACAGAACT TATTAAAGTG GATAAAGTGA AAATTGTAGC CTGTGATGGT 
AAAAATACCG GTTCCAATAC CACGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_445797.1
CDS	1..330
Translation	MAYQLYRNTTLGNSLQESLDELIQSQQITPQLALQVLLQFDKAINSALAQRVRNRVNFRGSLNTYRFCDNVWTFVLNDVEFREVTELIKVDKVKIVACDGKNTGSNTTE

Target ORF information:

RefSeq Version	NM_053345.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus general transcription factor 2A subunit 2 (Gtf2a2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053345.1

ORF Insert Sequence:

ATGGCATATC AGTTATACAG AAATACAACT TTGGGGAACA GTCTTCAAGA GAGCCTTGAT 
GAGCTCATAC AGTCTCAACA GATCACCCCC CAGCTTGCCC TTCAAGTTCT ACTTCAGTTT 
GATAAAGCTA TAAATTCAGC ATTGGCTCAG AGAGTCAGGA ACAGAGTCAA TTTCAGGGGC 
TCTCTAAATA CATACAGATT CTGCGATAAT GTTTGGACTT TTGTATTGAA TGATGTTGAA 
TTCAGAGAGG TGACAGAACT TATTAAAGTG GATAAAGTGA AAATTGTAGC CTGTGATGGT 
AAAAATACCG GTTCCAATAC CACGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gtf2a2 ( XM_006243380.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006243442.1 Rattus norvegicus general transcription factor IIA, 2 (Gtf2a2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa09322
Clone ID Related Accession (Same CDS sequence)	XM_006243380.3 , NM_053345.1
Accession Version	XM_006243380.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 330bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	transcription initiation factor IIA subunit 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006243380.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATATC AGTTATACAG AAATACAACT TTGGGGAACA GTCTTCAAGA GAGCCTTGAT 
GAGCTCATAC AGTCTCAACA GATCACCCCC CAGCTTGCCC TTCAAGTTCT ACTTCAGTTT 
GATAAAGCTA TAAATTCAGC ATTGGCTCAG AGAGTCAGGA ACAGAGTCAA TTTCAGGGGC 
TCTCTAAATA CATACAGATT CTGCGATAAT GTTTGGACTT TTGTATTGAA TGATGTTGAA 
TTCAGAGAGG TGACAGAACT TATTAAAGTG GATAAAGTGA AAATTGTAGC CTGTGATGGT 
AAAAATACCG GTTCCAATAC CACGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006243442.1
CDS	154..483
Translation	MAYQLYRNTTLGNSLQESLDELIQSQQITPQLALQVLLQFDKAINSALAQRVRNRVNFRGSLNTYRFCDNVWTFVLNDVEFREVTELIKVDKVKIVACDGKNTGSNTTE

Target ORF information:

RefSeq Version	XM_006243380.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus general transcription factor IIA, 2 (Gtf2a2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006243380.3

ORF Insert Sequence:

ATGGCATATC AGTTATACAG AAATACAACT TTGGGGAACA GTCTTCAAGA GAGCCTTGAT 
GAGCTCATAC AGTCTCAACA GATCACCCCC CAGCTTGCCC TTCAAGTTCT ACTTCAGTTT 
GATAAAGCTA TAAATTCAGC ATTGGCTCAG AGAGTCAGGA ACAGAGTCAA TTTCAGGGGC 
TCTCTAAATA CATACAGATT CTGCGATAAT GTTTGGACTT TTGTATTGAA TGATGTTGAA 
TTCAGAGAGG TGACAGAACT TATTAAAGTG GATAAAGTGA AAATTGTAGC CTGTGATGGT 
AAAAATACCG GTTCCAATAC CACGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Near-atomic resolution visualization of human transcription promoter opening.
Nature533(7603)359-65(2016 05)
He Y,Yan C,Fang J,Inouye C,Tjian R,Ivanov I,Nogales E

Structural insights into transcription initiation by RNA polymerase II.
Trends in biochemical sciences38(12)603-11(2013 Dec)
Gr?nberg S,Hahn S

The homeobox gene Mohawk represses transcription by recruiting the sin3A/HDAC co-repressor complex.
Developmental dynamics : an official publication of the American Association of Anatomists238(3)572-80(2009 Mar)
Anderson DM,Beres BJ,Wilson-Rawls J,Rawls A

General transcription factor IIA-gamma increases osteoblast-specific osteocalcin gene expression via activating transcription factor 4 and runt-related transcription factor 2.
The Journal of biological chemistry283(9)5542-53(2008 Feb)
Yu S,Jiang Y,Galson DL,Luo M,Lai Y,Lu Y,Ouyang HJ,Zhang J,Xiao G

TFIIAalpha/beta-like factor is encoded by a germ cell-specific gene whose expression is up-regulated with other general transcription factors during spermatogenesis in the mouse.
Biology of reproduction64(2)507-17(2001 Feb)
Han SY,Zhou L,Upadhyaya A,Lee SH,Parker KL,DeJong J