HECW2 cDNA ORF clone, Sus scrofa(pig)

  • Gene

  • Clones

  • gRNAs

The following HECW2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HECW2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSe239004 XM_021074546.1
Latest version!
Sus scrofa HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OSe01255 NM_001198924.1
Latest version!
Sus scrofa HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OSe01255 XM_021074544.1
Latest version!
Sus scrofa HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OSe01255 XM_021074543.1
Latest version!
Sus scrofa HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OSe01255 XM_021074541.1
Latest version!
Sus scrofa HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OSe01255 XM_021074545.1
Latest version!
Sus scrofa HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OSe01255 XM_021074542.1
Latest version!
Sus scrofa HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSe239004
    Clone ID Related Accession (Same CDS sequence) XM_021074546.1
    Accession Version XM_021074546.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4332bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product E3 ubiquitin-protein ligase HECW2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010457.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGAGCCGG AGATAAAAAT CTGTTTCAAA TACTACCACG GCATTAGTGG AGCCCTGCGA 
    GCCACGACCC CCTGCATCAC GGTGAAGAAC CCTGCTGTGA TGATGGGGGC AGAAGGCATG
    GAAGGAGGTG CTGCAGGCAG TCAGCATTCT CGGAAACTCG TTAGCTTTAC GTTGTCAGAT
    CTTAGGGCAG TTGGGCTAAA GAAAGGGATG TTCTTCAATC CTGACCCTTA TCTGAAGATG
    TCCATTCAGC CAGGGAAGAA GAGCAGTTTC CCCACCTGTG CCCACCATGG GCAGGAGAGG
    AGGTCTACCA TCATCAGTAA CACCACTAAT CCAATCTGGC ACCGAGAGAA ATACTCCTTT
    TTTGCACTCC TTACTGATGT CTTAGAAATT GAAATTAAAG ACAAATTTGC CAAGAGCCGT
    CCTATCATCA AGCGTTTTCT GGGGAAACTA ACCATCCCAG TCCAGAGGCT GTTGGAGCGA
    CAAGCCATCG GTGATCAGAT GCTCAGCTAC AACCTTGGCA GAAGGCTCCC AGCTGACCAC
    GTGAGTGGGT ACCTCCAGTT CAGAGTGGAG GTCACGTCTT CTGTGCATGA AGATGCTTCT
    CCAGAAGCAG TTGGCACAAT ACTTGGAGTC AATACTGTGA ATGGAGACCT AGGAAGCCCT
    TCTGATGAGG AGGACATGCC AGGGGGCCAC CACGACAGCC CGGTTTGTTC TAATGGACCA
    GTTTCTGAGG AAAGTGCTGA TGGGACCCCC AAGCATTCTT TCAGGACTAG CTCTACTCTG
    GAAATAGACA CGGAAGATTT AACCTCTACT TCTTCAAGGA CCTCACCTCC CAGAGGACGT
    CAGGATTCCC TCAACGATTA TTTAGATGCT ATTGAACACA ATGGCCATTC CAGGTCAGGT
    GCAGCTACCT GCTCAGAGAG GTCCATGGGA GCCTCTCCAA AACTAAGGAG TAGTTTTCCC
    ACCGACACGA GACTCAATGC CATGCTTCAT ATTGATTCAG ATGAGGAAGA CCACGAGTTC
    CAGCAGGACC TGGGTTATCC ATCTTCCTTG GAGGAGGAAG GAGGGTTGAT AATGTTTAGC
    AGGGCATCAA GAACTGATGA CGGGAGCCTC ACATCTCAGA CAAAGCCGGA AGACATCCCA
    GTGGAGACTG AGGAAGCCTC TATCCACGAA CCTGCTTCCT TTGAGGATAA GCCAGAACAT
    CTTCCGGAGC TTACAGAGAG CTCCTTACCC TCAGGCCCCA TGCCAGATGA AAGTGAAAAT
    GGCCTGGAGT CTCAACCAGG TGCCGACCAG GGCAGCACTG AATTGTGTGG CTCTCAGGAG
    GTCGATCAGC CTACGAGTGG GGCAGACACG GGCACTTCTG ACACGTCGGG AGGAAGCAGA
    AGAGCCATCA GTGAGACAGA GTCCCTTGAT CAGGGGTCCG AGCCTTCCCA GGTGTCCTCT
    GAAACAGAAC CCAGTGACCC TGCCAGGACA GAGAGCGTGA GTGAGGCCAG CACCAGGCCT
    GAGGGAGAGA GTGACCTGGA AGGGGCCGAC AGCTCCTGCA ATGAGAGTGT CACCACGCAG
    CTGTCCTCGG TGGAGACACG GTGCTCATCT CTTGAGAGTG CACGGTTTCC CGAAACCCCA
    GCCTTTTCTT CTCAGGAGGA GGAAGATGGA GCCTGTGCAG CAGAGCCCAC CAGCAGTGGC
    CCTGCCGAGG GGTCACAGGA TTCCATATGC ACTCCTGGGT CCTTACCTGT GGTGCAGGTG
    CCCAGTGGAG AGGAGGAAGG GCCAGGGGCA GAATCGGCCA CCTTACCTGA CCAGGAGGAG
    GTGGGGGAGG TCTGGCAGCG GAGGGGGAGC CTGGAGGGAG CTGCTGCTGC TGCTGCTGCT
    GCTGAGAGCC AGCCCCAAGA AGAGGGTGAT GCTGGGGAAG CCCAAGGGGC TTGTGAAGGG
    GCCACTGCCC AGGAGGAGGG TGCTACTGGA GGTTCTCAAG CCAACGGCCA CCAGCCACTG
    CGATCTCTGC CTTCAGTGCG CCAAGATGTT AGCCGGTACC AGAGGGTGGA CGAGGCTCTC
    CCACCAAACT GGGAGGCCCG CATTGACAGC CACGGCAGGA TCTTCTACGT GGATCACGTG
    AACAGAACCA CGACGTGGCA GCGGCCCACC GCTCCCCCCG CCCCGCAGGT GCTGCAGAGG
    TCCAACTCCA TACAGCAGAT GGAGCAGCTG AACCGGCGAT ACCAGAGTAT CCGCAGAACC
    ATGACTAACG AGAGGCCCGA GGAAAATAGC AGTGCTCTTG ATGGGGCAGG AGAGGAGGCC
    GACTTTCACC AAGCCAGTGC AGATTTCCGA CGGGAGAGCG TCCTGCCCCA CTCTACCTCC
    AGATCCAGGC TCACGTTGCT GTTACAGTCG CCCCCTGTGA AGTTCCTCAT CAGCCCAGAG
    TTCTTCACCG TGCTGCATTC GAACCCTAGT GCCTACCGCA TGTTTACAAA CAACACGTGT
    TTGAAGCACA TGATCACCAA AGTCCGGCGG GACACCCACC ACTTTGAACG CTACCAGCAT
    AACCGGGACC TTGTGGGATT CCTCAACATG TTTGCAAACA AACAACTGGA GCTGCCGAGG
    GGCTGGGAAA TGAAACACGA TCATCAGGGC AAGGCATTTT TTGTTGACCA CAACTCCCGG
    ACCACCACTT TCATTGACCC CCGGCTCCCA CTCCAGAGCA GCAGACCCAC GAGTGCGCTG
    GTGCATCGAC AGCATCTGAC GAGGCAGCGC AGCCACAGTG CAGGCGAGGT GGGAGAAGAT
    TCTCGACATG CCGGACCACC AGTTCTTCCC AGGCCGTCGA GTACGTTCAA CACAGTCAGC
    AGGCCGCAGT ACCAGGACAT GGTTCCAGTG GCTTACAATG ACAAGATTGT TGCATTTCTT
    CGCCAACCCA ACATCTTTGA AATTCTACAG GAGCGTCAAC CTGATCTTAC CAGGAATCAC
    TCACTCAGGG AGAAGATCCA ATTTATCCGA ACTGAAGGGA CCCCAGGATT GGTGCGTCTT
    TCAAGTGATG CAGACCTTGT TATGTTGCTA AGTTTATTTG AAGAAGAGAT AATGTCATAC
    GTGCCTCCAC ATGCCTTACT CCATCCCAGC TACTGTCAGT CCCCACGTGG CTCCCCTGTG
    TCCTCTCCCC AGAACTCGCC AGGTACTCAG CGTGCCAACG CCCGGGCTCC CGCCCCTTAC
    AAGCGAGACT TTGAAGCCAA ACTGAGGAAT TTTTACAGGA AGTTAGAGAC TAAAGGATAT
    GGACAAGGCC CAGGGAAATT AAAGTTAATT ATCAGAAGAG ATCACTTGCT CGAAGATGCT
    TTTAATCAGA TTATGGGCTA TTCCAGAAAA GACCTGCAGA GAAATAAGCT GTACGTCACA
    TTTGTCGGGG AGGAAGGGCT GGATTACAGT GGACCTTCCA GAGAGTTTTT CTTCCTGGTA
    TCCAGAGAGC TCTTTAACCC ATATTACGGC TTATTTGAAT ATTCAGCCAA TGACACATAC
    ACAGTACAAA TAAGTCCCAT GTCTGCTTTT GTAGACAATC ACCATGAATG GTTCCGGTTC
    AGTGGTAGGA TCCTTGGTCT TGCGCTGATT CACCAGTATT TGTTGGATGC CTTCTTCACG
    CGGCCCTTCT ATAAGGCTCT TCTCAGAATT CTGTGTGACC TGAGTGATCT AGAATACCTT
    GATGAGGAGT TCCACCAGAG CCTGCAGTGG ATGAAAGACA ATGATATCCA TGACATTCTG
    GACCTCACGT TCACAGTGAA CGAAGAAGTT TTCGGGCAGA TAACTGAACG AGAATTAAAA
    CCAGGGGGTG CCAACATCCC AGTCACAGAG AAGAACAAGA AGGAGTACAT CGAGAGGATG
    GTGAAATGGA GGATTGAGCG GGGTGTTGTG CAGCAAACGG AGAGCTTAGT GCGTGGCTTC
    TACGAGGTGG TGGATGCCAG GCTAGTATCT GTTTTTGATG CAAGAGAACT GGAACTGGTC
    ATCGCAGGCA CAGCTGAAAT AGACCTAAGT GATTGGAGAA ACAACACAGA ATACAGAGGA
    GGTTATCATG ACAATCATAT TGTAATTCGG TGGTTCTGGG CTGCAGTGGA GAGATTCAAC
    AATGAACAAC GATTAAGGTT GTTACAGTTT GTCACAGGCA CATCCAGCAT TCCCTATGAA
    GGATTTGCTT CTCTCCGAGG GAGTAATGGC CCAAGAAGAT TCTGCGTGGA GAAATGGGGG
    AAAATCACCG CTCTTCCCAG AGCTCACACT TGTTTTAACC GTCTGGATCT CCCTCCATAC
    CCATCCTTCT CCATGCTTTA TGAAAAACTT TTGACAGCAG TTGAGGAGAC CAGTACCTTT
    GGACTTGAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020930205.1
    CDS906..5237
    Translation

    Target ORF information:

    RefSeq Version XM_021074546.1
    Organism Sus scrofa(pig)
    Definition Sus scrofa HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021074546.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGAGCCGG AGATAAAAAT CTGTTTCAAA TACTACCACG GCATTAGTGG AGCCCTGCGA 
    GCCACGACCC CCTGCATCAC GGTGAAGAAC CCTGCTGTGA TGATGGGGGC AGAAGGCATG
    GAAGGAGGTG CTGCAGGCAG TCAGCATTCT CGGAAACTCG TTAGCTTTAC GTTGTCAGAT
    CTTAGGGCAG TTGGGCTAAA GAAAGGGATG TTCTTCAATC CTGACCCTTA TCTGAAGATG
    TCCATTCAGC CAGGGAAGAA GAGCAGTTTC CCCACCTGTG CCCACCATGG GCAGGAGAGG
    AGGTCTACCA TCATCAGTAA CACCACTAAT CCAATCTGGC ACCGAGAGAA ATACTCCTTT
    TTTGCACTCC TTACTGATGT CTTAGAAATT GAAATTAAAG ACAAATTTGC CAAGAGCCGT
    CCTATCATCA AGCGTTTTCT GGGGAAACTA ACCATCCCAG TCCAGAGGCT GTTGGAGCGA
    CAAGCCATCG GTGATCAGAT GCTCAGCTAC AACCTTGGCA GAAGGCTCCC AGCTGACCAC
    GTGAGTGGGT ACCTCCAGTT CAGAGTGGAG GTCACGTCTT CTGTGCATGA AGATGCTTCT
    CCAGAAGCAG TTGGCACAAT ACTTGGAGTC AATACTGTGA ATGGAGACCT AGGAAGCCCT
    TCTGATGAGG AGGACATGCC AGGGGGCCAC CACGACAGCC CGGTTTGTTC TAATGGACCA
    GTTTCTGAGG AAAGTGCTGA TGGGACCCCC AAGCATTCTT TCAGGACTAG CTCTACTCTG
    GAAATAGACA CGGAAGATTT AACCTCTACT TCTTCAAGGA CCTCACCTCC CAGAGGACGT
    CAGGATTCCC TCAACGATTA TTTAGATGCT ATTGAACACA ATGGCCATTC CAGGTCAGGT
    GCAGCTACCT GCTCAGAGAG GTCCATGGGA GCCTCTCCAA AACTAAGGAG TAGTTTTCCC
    ACCGACACGA GACTCAATGC CATGCTTCAT ATTGATTCAG ATGAGGAAGA CCACGAGTTC
    CAGCAGGACC TGGGTTATCC ATCTTCCTTG GAGGAGGAAG GAGGGTTGAT AATGTTTAGC
    AGGGCATCAA GAACTGATGA CGGGAGCCTC ACATCTCAGA CAAAGCCGGA AGACATCCCA
    GTGGAGACTG AGGAAGCCTC TATCCACGAA CCTGCTTCCT TTGAGGATAA GCCAGAACAT
    CTTCCGGAGC TTACAGAGAG CTCCTTACCC TCAGGCCCCA TGCCAGATGA AAGTGAAAAT
    GGCCTGGAGT CTCAACCAGG TGCCGACCAG GGCAGCACTG AATTGTGTGG CTCTCAGGAG
    GTCGATCAGC CTACGAGTGG GGCAGACACG GGCACTTCTG ACACGTCGGG AGGAAGCAGA
    AGAGCCATCA GTGAGACAGA GTCCCTTGAT CAGGGGTCCG AGCCTTCCCA GGTGTCCTCT
    GAAACAGAAC CCAGTGACCC TGCCAGGACA GAGAGCGTGA GTGAGGCCAG CACCAGGCCT
    GAGGGAGAGA GTGACCTGGA AGGGGCCGAC AGCTCCTGCA ATGAGAGTGT CACCACGCAG
    CTGTCCTCGG TGGAGACACG GTGCTCATCT CTTGAGAGTG CACGGTTTCC CGAAACCCCA
    GCCTTTTCTT CTCAGGAGGA GGAAGATGGA GCCTGTGCAG CAGAGCCCAC CAGCAGTGGC
    CCTGCCGAGG GGTCACAGGA TTCCATATGC ACTCCTGGGT CCTTACCTGT GGTGCAGGTG
    CCCAGTGGAG AGGAGGAAGG GCCAGGGGCA GAATCGGCCA CCTTACCTGA CCAGGAGGAG
    GTGGGGGAGG TCTGGCAGCG GAGGGGGAGC CTGGAGGGAG CTGCTGCTGC TGCTGCTGCT
    GCTGAGAGCC AGCCCCAAGA AGAGGGTGAT GCTGGGGAAG CCCAAGGGGC TTGTGAAGGG
    GCCACTGCCC AGGAGGAGGG TGCTACTGGA GGTTCTCAAG CCAACGGCCA CCAGCCACTG
    CGATCTCTGC CTTCAGTGCG CCAAGATGTT AGCCGGTACC AGAGGGTGGA CGAGGCTCTC
    CCACCAAACT GGGAGGCCCG CATTGACAGC CACGGCAGGA TCTTCTACGT GGATCACGTG
    AACAGAACCA CGACGTGGCA GCGGCCCACC GCTCCCCCCG CCCCGCAGGT GCTGCAGAGG
    TCCAACTCCA TACAGCAGAT GGAGCAGCTG AACCGGCGAT ACCAGAGTAT CCGCAGAACC
    ATGACTAACG AGAGGCCCGA GGAAAATAGC AGTGCTCTTG ATGGGGCAGG AGAGGAGGCC
    GACTTTCACC AAGCCAGTGC AGATTTCCGA CGGGAGAGCG TCCTGCCCCA CTCTACCTCC
    AGATCCAGGC TCACGTTGCT GTTACAGTCG CCCCCTGTGA AGTTCCTCAT CAGCCCAGAG
    TTCTTCACCG TGCTGCATTC GAACCCTAGT GCCTACCGCA TGTTTACAAA CAACACGTGT
    TTGAAGCACA TGATCACCAA AGTCCGGCGG GACACCCACC ACTTTGAACG CTACCAGCAT
    AACCGGGACC TTGTGGGATT CCTCAACATG TTTGCAAACA AACAACTGGA GCTGCCGAGG
    GGCTGGGAAA TGAAACACGA TCATCAGGGC AAGGCATTTT TTGTTGACCA CAACTCCCGG
    ACCACCACTT TCATTGACCC CCGGCTCCCA CTCCAGAGCA GCAGACCCAC GAGTGCGCTG
    GTGCATCGAC AGCATCTGAC GAGGCAGCGC AGCCACAGTG CAGGCGAGGT GGGAGAAGAT
    TCTCGACATG CCGGACCACC AGTTCTTCCC AGGCCGTCGA GTACGTTCAA CACAGTCAGC
    AGGCCGCAGT ACCAGGACAT GGTTCCAGTG GCTTACAATG ACAAGATTGT TGCATTTCTT
    CGCCAACCCA ACATCTTTGA AATTCTACAG GAGCGTCAAC CTGATCTTAC CAGGAATCAC
    TCACTCAGGG AGAAGATCCA ATTTATCCGA ACTGAAGGGA CCCCAGGATT GGTGCGTCTT
    TCAAGTGATG CAGACCTTGT TATGTTGCTA AGTTTATTTG AAGAAGAGAT AATGTCATAC
    GTGCCTCCAC ATGCCTTACT CCATCCCAGC TACTGTCAGT CCCCACGTGG CTCCCCTGTG
    TCCTCTCCCC AGAACTCGCC AGGTACTCAG CGTGCCAACG CCCGGGCTCC CGCCCCTTAC
    AAGCGAGACT TTGAAGCCAA ACTGAGGAAT TTTTACAGGA AGTTAGAGAC TAAAGGATAT
    GGACAAGGCC CAGGGAAATT AAAGTTAATT ATCAGAAGAG ATCACTTGCT CGAAGATGCT
    TTTAATCAGA TTATGGGCTA TTCCAGAAAA GACCTGCAGA GAAATAAGCT GTACGTCACA
    TTTGTCGGGG AGGAAGGGCT GGATTACAGT GGACCTTCCA GAGAGTTTTT CTTCCTGGTA
    TCCAGAGAGC TCTTTAACCC ATATTACGGC TTATTTGAAT ATTCAGCCAA TGACACATAC
    ACAGTACAAA TAAGTCCCAT GTCTGCTTTT GTAGACAATC ACCATGAATG GTTCCGGTTC
    AGTGGTAGGA TCCTTGGTCT TGCGCTGATT CACCAGTATT TGTTGGATGC CTTCTTCACG
    CGGCCCTTCT ATAAGGCTCT TCTCAGAATT CTGTGTGACC TGAGTGATCT AGAATACCTT
    GATGAGGAGT TCCACCAGAG CCTGCAGTGG ATGAAAGACA ATGATATCCA TGACATTCTG
    GACCTCACGT TCACAGTGAA CGAAGAAGTT TTCGGGCAGA TAACTGAACG AGAATTAAAA
    CCAGGGGGTG CCAACATCCC AGTCACAGAG AAGAACAAGA AGGAGTACAT CGAGAGGATG
    GTGAAATGGA GGATTGAGCG GGGTGTTGTG CAGCAAACGG AGAGCTTAGT GCGTGGCTTC
    TACGAGGTGG TGGATGCCAG GCTAGTATCT GTTTTTGATG CAAGAGAACT GGAACTGGTC
    ATCGCAGGCA CAGCTGAAAT AGACCTAAGT GATTGGAGAA ACAACACAGA ATACAGAGGA
    GGTTATCATG ACAATCATAT TGTAATTCGG TGGTTCTGGG CTGCAGTGGA GAGATTCAAC
    AATGAACAAC GATTAAGGTT GTTACAGTTT GTCACAGGCA CATCCAGCAT TCCCTATGAA
    GGATTTGCTT CTCTCCGAGG GAGTAATGGC CCAAGAAGAT TCTGCGTGGA GAAATGGGGG
    AAAATCACCG CTCTTCCCAG AGCTCACACT TGTTTTAACC GTCTGGATCT CCCTCCATAC
    CCATCCTTCT CCATGCTTTA TGAAAAACTT TTGACAGCAG TTGAGGAGAC CAGTACCTTT
    GGACTTGAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe01255
    Clone ID Related Accession (Same CDS sequence) XM_021074541.1 , XM_021074543.1 , NM_001198924.1 , XM_021074542.1 , XM_021074545.1 , XM_021074544.1
    Accession Version NM_001198924.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4725bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-15
    Organism Sus scrofa(pig)
    Product E3 ubiquitin-protein ligase HECW2
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from HM562353.1. ##Evidence-Data-START## Transcript exon combination :: HM562353.1 [ECO:0000332] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCTAGCT CAGCCCGGGA GCACCTGCTT TTTGTGCGGC GGCGAAATCC CCAGATGCGG 
    TACACTCTGA GTCCAGAGAA CCTCCAGAGC CTGGCTGCCC AGAGCTCCAT GCCTGAGAAC
    ATGACCCTGC AGCGGGCCAA CAGCGACACA GATCTGGTGA CTTCTGAGAG CCGGTCCAGC
    CTGACTGCCA GCATGTATGA GTACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG
    GACATTAAAG AGGAGGTGGA CCCGAGTGAT TGGATTGGAC TTTATCACAT AGATGAGAAT
    TCTCCAGCCA ACTTCTGGGA TTCTAAAAAC AGGGGTGTGA CTGGAACACA AAAAGGACAA
    ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAGC CGGAGATAAA AATCTGTTTC
    AAATACTACC ACGGCATTAG TGGAGCCCTG CGAGCCACGA CCCCCTGCAT CACGGTGAAG
    AACCCTGCTG TGATGATGGG GGCAGAAGGC ATGGAAGGAG GTGCTGCAGG CAGTCAGCAT
    TCTCGGAAAC TCGTTAGCTT TACGTTGTCA GATCTTAGGG CAGTTGGGCT AAAGAAAGGG
    ATGTTCTTCA ATCCTGACCC TTATCTGAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT
    TTCCCCACCT GTGCCCACCA TGGGCAGGAG AGGAGGTCTA CCATCATCAG TAACACCACT
    AATCCAATCT GGCACCGAGA GAAATACTCC TTTTTTGCAC TCCTTACTGA TGTCTTAGAA
    ATTGAAATTA AAGACAAATT TGCCAAGAGC CGTCCTATCA TCAAGCGTTT TCTGGGGAAA
    CTAACCATCC CAGTCCAGAG GCTGTTGGAG CGACAAGCCA TCGGTGATCA GATGCTCAGC
    TACAACCTTG GCAGAAGGCT CCCAGCTGAC CACGTGAGTG GGTACCTCCA GTTCAGAGTG
    GAGGTCACGT CTTCTGTGCA TGAAGATGCT TCTCCAGAAG CAGTTGGCAC AATACTTGGA
    GTCAATACTG TGAATGGAGA CCTAGGAAGC CCTTCTGATG AGGAGGACAT GCCAGGGGGC
    CACCACGACA GCCCGGTTTG TTCTAATGGA CCAGTTTCTG AGGAAAGTGC TGATGGGACC
    CCCAAGCATT CTTTCAGGAC TAGCTCTACT CTGGAAATAG ACACGGAAGA TTTAACCTCT
    ACTTCTTCAA GGACCTCACC TCCCAGAGGA CGTCAGGATT CCCTCAACGA TTATTTAGAT
    GCTATTGAAC ACAATGGCCA TTCCAGGTCA GGTGCAGCTA CCTGCTCAGA GAGGTCCATG
    GGAGCCTCTC CAAAACTAAG GAGTAGTTTT CCCACCGACA CGAGACTCAA TGCCATGCTT
    CATATTGATT CAGATGAGGA AGACCACGAG TTCCAGCAGG ACCTGGGTTA TCCATCTTCC
    TTGGAGGAGG AAGGAGGGTT GATAATGTTT AGCAGGGCAT CAAGAACTGA TGACGGGAGC
    CTCACATCTC AGACAAAGCC GGAAGACATC CCAGTGGAGA CTGAGGAAGC CTCTATCCAC
    GAACCTGCTT CCTTTGAGGA TAAGCCAGAA CATCTTCCGG AGCTTACAGA GAGCTCCTTA
    CCCTCAGGCC CCATGCCAGA TGAAAGTGAA AATGGCCTGG AGTCTCAACC AGGTGCCGAC
    CAGGGCAGCA CTGAATTGTG TGGCTCTCAG GAGGTCGATC AGCCTACGAG TGGGGCAGAC
    ACGGGCACTT CTGACACGTC GGGAGGAAGC AGAAGAGCCA TCAGTGAGAC AGAGTCCCTT
    GATCAGGGGT CCGAGCCTTC CCAGGTGTCC TCTGAAACAG AACCCAGTGA CCCTGCCAGG
    ACAGAGAGCG TGAGTGAGGC CAGCACCAGG CCTGAGGGAG AGAGTGACCT GGAAGGGGCC
    GACAGCTCCT GCAATGAGAG TGTCACCACG CAGCTGTCCT CGGTGGAGAC ACGGTGCTCA
    TCTCTTGAGA GTGCACGGTT TCCCGAAACC CCAGCCTTTT CTTCTCAGGA GGAGGAAGAT
    GGAGCCTGTG CAGCAGAGCC CACCAGCAGT GGCCCTGCCG AGGGGTCACA GGATTCCATA
    TGCACTCCTG GGTCCTTACC TGTGGTGCAG GTGCCCAGTG GAGAGGAGGA AGGGCCAGGG
    GCAGAATCGG CCACCTTACC TGACCAGGAG GAGGTGGGGG AGGTCTGGCA GCGGAGGGGG
    AGCCTGGAGG GAGCTGCTGC TGCTGCTGCT GCTGCTGAGA GCCAGCCCCA AGAAGAGGGT
    GATGCTGGGG AAGCCCAAGG GGCTTGTGAA GGGGCCACTG CCCAGGAGGA GGGTGCTACT
    GGAGGTTCTC AAGCCAACGG CCACCAGCCA CTGCGATCTC TGCCTTCAGT GCGCCAAGAT
    GTTAGCCGGT ACCAGAGGGT GGACGAGGCT CTCCCACCAA ACTGGGAGGC CCGCATTGAC
    AGCCACGGCA GGATCTTCTA CGTGGATCAC GTGAACAGAA CCACGACGTG GCAGCGGCCC
    ACCGCTCCCC CCGCCCCGCA GGTGCTGCAG AGGTCCAACT CCATACAGCA GATGGAGCAG
    CTGAACCGGC GATACCAGAG TATCCGCAGA ACCATGACTA ACGAGAGGCC CGAGGAAAAT
    AGCAGTGCTC TTGATGGGGC AGGAGAGGAG GCCGACTTTC ACCAAGCCAG TGCAGATTTC
    CGACGGGAGA GCGTCCTGCC CCACTCTACC TCCAGATCCA GGCTCACGTT GCTGTTACAG
    TCGCCCCCTG TGAAGTTCCT CATCAGCCCA GAGTTCTTCA CCGTGCTGCA TTCGAACCCT
    AGTGCCTACC GCATGTTTAC AAACAACACG TGTTTGAAGC ACATGATCAC CAAAGTCCGG
    CGGGACACCC ACCACTTTGA ACGCTACCAG CATAACCGGG ACCTTGTGGG ATTCCTCAAC
    ATGTTTGCAA ACAAACAACT GGAGCTGCCG AGGGGCTGGG AAATGAAACA CGATCATCAG
    GGCAAGGCAT TTTTTGTTGA CCACAACTCC CGGACCACCA CTTTCATTGA CCCCCGGCTC
    CCACTCCAGA GCAGCAGACC CACGAGTGCG CTGGTGCATC GACAGCATCT GACGAGGCAG
    CGCAGCCACA GTGCAGGCGA GGTGGGAGAA GATTCTCGAC ATGCCGGACC ACCAGTTCTT
    CCCAGGCCGT CGAGTACGTT CAACACAGTC AGCAGGCCGC AGTACCAGGA CATGGTTCCA
    GTGGCTTACA ATGACAAGAT TGTTGCATTT CTTCGCCAAC CCAACATCTT TGAAATTCTA
    CAGGAGCGTC AACCTGATCT TACCAGGAAT CACTCACTCA GGGAGAAGAT CCAATTTATC
    CGAACTGAAG GGACCCCAGG ATTGGTGCGT CTTTCAAGTG ATGCAGACCT TGTTATGTTG
    CTAAGTTTAT TTGAAGAAGA GATAATGTCA TACGTGCCTC CACATGCCTT ACTCCATCCC
    AGCTACTGTC AGTCCCCACG TGGCTCCCCT GTGTCCTCTC CCCAGAACTC GCCAGGTACT
    CAGCGTGCCA ACGCCCGGGC TCCCGCCCCT TACAAGCGAG ACTTTGAAGC CAAACTGAGG
    AATTTTTACA GGAAGTTAGA GACTAAAGGA TATGGACAAG GCCCAGGGAA ATTAAAGTTA
    ATTATCAGAA GAGATCACTT GCTCGAAGAT GCTTTTAATC AGATTATGGG CTATTCCAGA
    AAAGACCTGC AGAGAAATAA GCTGTACGTC ACATTTGTCG GGGAGGAAGG GCTGGATTAC
    AGTGGACCTT CCAGAGAGTT TTTCTTCCTG GTATCCAGAG AGCTCTTTAA CCCATATTAC
    GGCTTATTTG AATATTCAGC CAATGACACA TACACAGTAC AAATAAGTCC CATGTCTGCT
    TTTGTAGACA ATCACCATGA ATGGTTCCGG TTCAGTGGTA GGATCCTTGG TCTTGCGCTG
    ATTCACCAGT ATTTGTTGGA TGCCTTCTTC ACGCGGCCCT TCTATAAGGC TCTTCTCAGA
    ATTCTGTGTG ACCTGAGTGA TCTAGAATAC CTTGATGAGG AGTTCCACCA GAGCCTGCAG
    TGGATGAAAG ACAATGATAT CCATGACATT CTGGACCTCA CGTTCACAGT GAACGAAGAA
    GTTTTCGGGC AGATAACTGA ACGAGAATTA AAACCAGGGG GTGCCAACAT CCCAGTCACA
    GAGAAGAACA AGAAGGAGTA CATCGAGAGG ATGGTGAAAT GGAGGATTGA GCGGGGTGTT
    GTGCAGCAAA CGGAGAGCTT AGTGCGTGGC TTCTACGAGG TGGTGGATGC CAGGCTAGTA
    TCTGTTTTTG ATGCAAGAGA ACTGGAACTG GTCATCGCAG GCACAGCTGA AATAGACCTA
    AGTGATTGGA GAAACAACAC AGAATACAGA GGAGGTTATC ATGACAATCA TATTGTAATT
    CGGTGGTTCT GGGCTGCAGT GGAGAGATTC AACAATGAAC AACGATTAAG GTTGTTACAG
    TTTGTCACAG GCACATCCAG CATTCCCTAT GAAGGATTTG CTTCTCTCCG AGGGAGTAAT
    GGCCCAAGAA GATTCTGCGT GGAGAAATGG GGGAAAATCA CCGCTCTTCC CAGAGCTCAC
    ACTTGTTTTA ACCGTCTGGA TCTCCCTCCA TACCCATCCT TCTCCATGCT TTATGAAAAA
    CTTTTGACAG CAGTTGAGGA GACCAGTACC TTTGGACTTG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001185853.1
    CDS3..4727
    Translation

    Target ORF information:

    RefSeq Version NM_001198924.1
    Organism Sus scrofa(pig)
    Definition Sus scrofa HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001198924.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCTAGCT CAGCCCGGGA GCACCTGCTT TTTGTGCGGC GGCGAAATCC CCAGATGCGG 
    TACACTCTGA GTCCAGAGAA CCTCCAGAGC CTGGCTGCCC AGAGCTCCAT GCCTGAGAAC
    ATGACCCTGC AGCGGGCCAA CAGCGACACA GATCTGGTGA CTTCTGAGAG CCGGTCCAGC
    CTGACTGCCA GCATGTATGA GTACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG
    GACATTAAAG AGGAGGTGGA CCCGAGTGAT TGGATTGGAC TTTATCACAT AGATGAGAAT
    TCTCCAGCCA ACTTCTGGGA TTCTAAAAAC AGGGGTGTGA CTGGAACACA AAAAGGACAA
    ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAGC CGGAGATAAA AATCTGTTTC
    AAATACTACC ACGGCATTAG TGGAGCCCTG CGAGCCACGA CCCCCTGCAT CACGGTGAAG
    AACCCTGCTG TGATGATGGG GGCAGAAGGC ATGGAAGGAG GTGCTGCAGG CAGTCAGCAT
    TCTCGGAAAC TCGTTAGCTT TACGTTGTCA GATCTTAGGG CAGTTGGGCT AAAGAAAGGG
    ATGTTCTTCA ATCCTGACCC TTATCTGAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT
    TTCCCCACCT GTGCCCACCA TGGGCAGGAG AGGAGGTCTA CCATCATCAG TAACACCACT
    AATCCAATCT GGCACCGAGA GAAATACTCC TTTTTTGCAC TCCTTACTGA TGTCTTAGAA
    ATTGAAATTA AAGACAAATT TGCCAAGAGC CGTCCTATCA TCAAGCGTTT TCTGGGGAAA
    CTAACCATCC CAGTCCAGAG GCTGTTGGAG CGACAAGCCA TCGGTGATCA GATGCTCAGC
    TACAACCTTG GCAGAAGGCT CCCAGCTGAC CACGTGAGTG GGTACCTCCA GTTCAGAGTG
    GAGGTCACGT CTTCTGTGCA TGAAGATGCT TCTCCAGAAG CAGTTGGCAC AATACTTGGA
    GTCAATACTG TGAATGGAGA CCTAGGAAGC CCTTCTGATG AGGAGGACAT GCCAGGGGGC
    CACCACGACA GCCCGGTTTG TTCTAATGGA CCAGTTTCTG AGGAAAGTGC TGATGGGACC
    CCCAAGCATT CTTTCAGGAC TAGCTCTACT CTGGAAATAG ACACGGAAGA TTTAACCTCT
    ACTTCTTCAA GGACCTCACC TCCCAGAGGA CGTCAGGATT CCCTCAACGA TTATTTAGAT
    GCTATTGAAC ACAATGGCCA TTCCAGGTCA GGTGCAGCTA CCTGCTCAGA GAGGTCCATG
    GGAGCCTCTC CAAAACTAAG GAGTAGTTTT CCCACCGACA CGAGACTCAA TGCCATGCTT
    CATATTGATT CAGATGAGGA AGACCACGAG TTCCAGCAGG ACCTGGGTTA TCCATCTTCC
    TTGGAGGAGG AAGGAGGGTT GATAATGTTT AGCAGGGCAT CAAGAACTGA TGACGGGAGC
    CTCACATCTC AGACAAAGCC GGAAGACATC CCAGTGGAGA CTGAGGAAGC CTCTATCCAC
    GAACCTGCTT CCTTTGAGGA TAAGCCAGAA CATCTTCCGG AGCTTACAGA GAGCTCCTTA
    CCCTCAGGCC CCATGCCAGA TGAAAGTGAA AATGGCCTGG AGTCTCAACC AGGTGCCGAC
    CAGGGCAGCA CTGAATTGTG TGGCTCTCAG GAGGTCGATC AGCCTACGAG TGGGGCAGAC
    ACGGGCACTT CTGACACGTC GGGAGGAAGC AGAAGAGCCA TCAGTGAGAC AGAGTCCCTT
    GATCAGGGGT CCGAGCCTTC CCAGGTGTCC TCTGAAACAG AACCCAGTGA CCCTGCCAGG
    ACAGAGAGCG TGAGTGAGGC CAGCACCAGG CCTGAGGGAG AGAGTGACCT GGAAGGGGCC
    GACAGCTCCT GCAATGAGAG TGTCACCACG CAGCTGTCCT CGGTGGAGAC ACGGTGCTCA
    TCTCTTGAGA GTGCACGGTT TCCCGAAACC CCAGCCTTTT CTTCTCAGGA GGAGGAAGAT
    GGAGCCTGTG CAGCAGAGCC CACCAGCAGT GGCCCTGCCG AGGGGTCACA GGATTCCATA
    TGCACTCCTG GGTCCTTACC TGTGGTGCAG GTGCCCAGTG GAGAGGAGGA AGGGCCAGGG
    GCAGAATCGG CCACCTTACC TGACCAGGAG GAGGTGGGGG AGGTCTGGCA GCGGAGGGGG
    AGCCTGGAGG GAGCTGCTGC TGCTGCTGCT GCTGCTGAGA GCCAGCCCCA AGAAGAGGGT
    GATGCTGGGG AAGCCCAAGG GGCTTGTGAA GGGGCCACTG CCCAGGAGGA GGGTGCTACT
    GGAGGTTCTC AAGCCAACGG CCACCAGCCA CTGCGATCTC TGCCTTCAGT GCGCCAAGAT
    GTTAGCCGGT ACCAGAGGGT GGACGAGGCT CTCCCACCAA ACTGGGAGGC CCGCATTGAC
    AGCCACGGCA GGATCTTCTA CGTGGATCAC GTGAACAGAA CCACGACGTG GCAGCGGCCC
    ACCGCTCCCC CCGCCCCGCA GGTGCTGCAG AGGTCCAACT CCATACAGCA GATGGAGCAG
    CTGAACCGGC GATACCAGAG TATCCGCAGA ACCATGACTA ACGAGAGGCC CGAGGAAAAT
    AGCAGTGCTC TTGATGGGGC AGGAGAGGAG GCCGACTTTC ACCAAGCCAG TGCAGATTTC
    CGACGGGAGA GCGTCCTGCC CCACTCTACC TCCAGATCCA GGCTCACGTT GCTGTTACAG
    TCGCCCCCTG TGAAGTTCCT CATCAGCCCA GAGTTCTTCA CCGTGCTGCA TTCGAACCCT
    AGTGCCTACC GCATGTTTAC AAACAACACG TGTTTGAAGC ACATGATCAC CAAAGTCCGG
    CGGGACACCC ACCACTTTGA ACGCTACCAG CATAACCGGG ACCTTGTGGG ATTCCTCAAC
    ATGTTTGCAA ACAAACAACT GGAGCTGCCG AGGGGCTGGG AAATGAAACA CGATCATCAG
    GGCAAGGCAT TTTTTGTTGA CCACAACTCC CGGACCACCA CTTTCATTGA CCCCCGGCTC
    CCACTCCAGA GCAGCAGACC CACGAGTGCG CTGGTGCATC GACAGCATCT GACGAGGCAG
    CGCAGCCACA GTGCAGGCGA GGTGGGAGAA GATTCTCGAC ATGCCGGACC ACCAGTTCTT
    CCCAGGCCGT CGAGTACGTT CAACACAGTC AGCAGGCCGC AGTACCAGGA CATGGTTCCA
    GTGGCTTACA ATGACAAGAT TGTTGCATTT CTTCGCCAAC CCAACATCTT TGAAATTCTA
    CAGGAGCGTC AACCTGATCT TACCAGGAAT CACTCACTCA GGGAGAAGAT CCAATTTATC
    CGAACTGAAG GGACCCCAGG ATTGGTGCGT CTTTCAAGTG ATGCAGACCT TGTTATGTTG
    CTAAGTTTAT TTGAAGAAGA GATAATGTCA TACGTGCCTC CACATGCCTT ACTCCATCCC
    AGCTACTGTC AGTCCCCACG TGGCTCCCCT GTGTCCTCTC CCCAGAACTC GCCAGGTACT
    CAGCGTGCCA ACGCCCGGGC TCCCGCCCCT TACAAGCGAG ACTTTGAAGC CAAACTGAGG
    AATTTTTACA GGAAGTTAGA GACTAAAGGA TATGGACAAG GCCCAGGGAA ATTAAAGTTA
    ATTATCAGAA GAGATCACTT GCTCGAAGAT GCTTTTAATC AGATTATGGG CTATTCCAGA
    AAAGACCTGC AGAGAAATAA GCTGTACGTC ACATTTGTCG GGGAGGAAGG GCTGGATTAC
    AGTGGACCTT CCAGAGAGTT TTTCTTCCTG GTATCCAGAG AGCTCTTTAA CCCATATTAC
    GGCTTATTTG AATATTCAGC CAATGACACA TACACAGTAC AAATAAGTCC CATGTCTGCT
    TTTGTAGACA ATCACCATGA ATGGTTCCGG TTCAGTGGTA GGATCCTTGG TCTTGCGCTG
    ATTCACCAGT ATTTGTTGGA TGCCTTCTTC ACGCGGCCCT TCTATAAGGC TCTTCTCAGA
    ATTCTGTGTG ACCTGAGTGA TCTAGAATAC CTTGATGAGG AGTTCCACCA GAGCCTGCAG
    TGGATGAAAG ACAATGATAT CCATGACATT CTGGACCTCA CGTTCACAGT GAACGAAGAA
    GTTTTCGGGC AGATAACTGA ACGAGAATTA AAACCAGGGG GTGCCAACAT CCCAGTCACA
    GAGAAGAACA AGAAGGAGTA CATCGAGAGG ATGGTGAAAT GGAGGATTGA GCGGGGTGTT
    GTGCAGCAAA CGGAGAGCTT AGTGCGTGGC TTCTACGAGG TGGTGGATGC CAGGCTAGTA
    TCTGTTTTTG ATGCAAGAGA ACTGGAACTG GTCATCGCAG GCACAGCTGA AATAGACCTA
    AGTGATTGGA GAAACAACAC AGAATACAGA GGAGGTTATC ATGACAATCA TATTGTAATT
    CGGTGGTTCT GGGCTGCAGT GGAGAGATTC AACAATGAAC AACGATTAAG GTTGTTACAG
    TTTGTCACAG GCACATCCAG CATTCCCTAT GAAGGATTTG CTTCTCTCCG AGGGAGTAAT
    GGCCCAAGAA GATTCTGCGT GGAGAAATGG GGGAAAATCA CCGCTCTTCC CAGAGCTCAC
    ACTTGTTTTA ACCGTCTGGA TCTCCCTCCA TACCCATCCT TCTCCATGCT TTATGAAAAA
    CTTTTGACAG CAGTTGAGGA GACCAGTACC TTTGGACTTG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe01255
    Clone ID Related Accession (Same CDS sequence) XM_021074541.1 , XM_021074543.1 , NM_001198924.1 , XM_021074542.1 , XM_021074545.1 , XM_021074544.1
    Accession Version XM_021074541.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4725bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product E3 ubiquitin-protein ligase HECW2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010457.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCTAGCT CAGCCCGGGA GCACCTGCTT TTTGTGCGGC GGCGAAATCC CCAGATGCGG 
    TACACTCTGA GTCCAGAGAA CCTCCAGAGC CTGGCTGCCC AGAGCTCCAT GCCTGAGAAC
    ATGACCCTGC AGCGGGCCAA CAGCGACACA GATCTGGTGA CTTCTGAGAG CCGGTCCAGC
    CTGACTGCCA GCATGTATGA GTACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG
    GACATTAAAG AGGAGGTGGA CCCGAGTGAT TGGATTGGAC TTTATCACAT AGATGAGAAT
    TCTCCAGCCA ACTTCTGGGA TTCTAAAAAC AGGGGTGTGA CTGGAACACA AAAAGGACAA
    ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAGC CGGAGATAAA AATCTGTTTC
    AAATACTACC ACGGCATTAG TGGAGCCCTG CGAGCCACGA CCCCCTGCAT CACGGTGAAG
    AACCCTGCTG TGATGATGGG GGCAGAAGGC ATGGAAGGAG GTGCTGCAGG CAGTCAGCAT
    TCTCGGAAAC TCGTTAGCTT TACGTTGTCA GATCTTAGGG CAGTTGGGCT AAAGAAAGGG
    ATGTTCTTCA ATCCTGACCC TTATCTGAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT
    TTCCCCACCT GTGCCCACCA TGGGCAGGAG AGGAGGTCTA CCATCATCAG TAACACCACT
    AATCCAATCT GGCACCGAGA GAAATACTCC TTTTTTGCAC TCCTTACTGA TGTCTTAGAA
    ATTGAAATTA AAGACAAATT TGCCAAGAGC CGTCCTATCA TCAAGCGTTT TCTGGGGAAA
    CTAACCATCC CAGTCCAGAG GCTGTTGGAG CGACAAGCCA TCGGTGATCA GATGCTCAGC
    TACAACCTTG GCAGAAGGCT CCCAGCTGAC CACGTGAGTG GGTACCTCCA GTTCAGAGTG
    GAGGTCACGT CTTCTGTGCA TGAAGATGCT TCTCCAGAAG CAGTTGGCAC AATACTTGGA
    GTCAATACTG TGAATGGAGA CCTAGGAAGC CCTTCTGATG AGGAGGACAT GCCAGGGGGC
    CACCACGACA GCCCGGTTTG TTCTAATGGA CCAGTTTCTG AGGAAAGTGC TGATGGGACC
    CCCAAGCATT CTTTCAGGAC TAGCTCTACT CTGGAAATAG ACACGGAAGA TTTAACCTCT
    ACTTCTTCAA GGACCTCACC TCCCAGAGGA CGTCAGGATT CCCTCAACGA TTATTTAGAT
    GCTATTGAAC ACAATGGCCA TTCCAGGTCA GGTGCAGCTA CCTGCTCAGA GAGGTCCATG
    GGAGCCTCTC CAAAACTAAG GAGTAGTTTT CCCACCGACA CGAGACTCAA TGCCATGCTT
    CATATTGATT CAGATGAGGA AGACCACGAG TTCCAGCAGG ACCTGGGTTA TCCATCTTCC
    TTGGAGGAGG AAGGAGGGTT GATAATGTTT AGCAGGGCAT CAAGAACTGA TGACGGGAGC
    CTCACATCTC AGACAAAGCC GGAAGACATC CCAGTGGAGA CTGAGGAAGC CTCTATCCAC
    GAACCTGCTT CCTTTGAGGA TAAGCCAGAA CATCTTCCGG AGCTTACAGA GAGCTCCTTA
    CCCTCAGGCC CCATGCCAGA TGAAAGTGAA AATGGCCTGG AGTCTCAACC AGGTGCCGAC
    CAGGGCAGCA CTGAATTGTG TGGCTCTCAG GAGGTCGATC AGCCTACGAG TGGGGCAGAC
    ACGGGCACTT CTGACACGTC GGGAGGAAGC AGAAGAGCCA TCAGTGAGAC AGAGTCCCTT
    GATCAGGGGT CCGAGCCTTC CCAGGTGTCC TCTGAAACAG AACCCAGTGA CCCTGCCAGG
    ACAGAGAGCG TGAGTGAGGC CAGCACCAGG CCTGAGGGAG AGAGTGACCT GGAAGGGGCC
    GACAGCTCCT GCAATGAGAG TGTCACCACG CAGCTGTCCT CGGTGGAGAC ACGGTGCTCA
    TCTCTTGAGA GTGCACGGTT TCCCGAAACC CCAGCCTTTT CTTCTCAGGA GGAGGAAGAT
    GGAGCCTGTG CAGCAGAGCC CACCAGCAGT GGCCCTGCCG AGGGGTCACA GGATTCCATA
    TGCACTCCTG GGTCCTTACC TGTGGTGCAG GTGCCCAGTG GAGAGGAGGA AGGGCCAGGG
    GCAGAATCGG CCACCTTACC TGACCAGGAG GAGGTGGGGG AGGTCTGGCA GCGGAGGGGG
    AGCCTGGAGG GAGCTGCTGC TGCTGCTGCT GCTGCTGAGA GCCAGCCCCA AGAAGAGGGT
    GATGCTGGGG AAGCCCAAGG GGCTTGTGAA GGGGCCACTG CCCAGGAGGA GGGTGCTACT
    GGAGGTTCTC AAGCCAACGG CCACCAGCCA CTGCGATCTC TGCCTTCAGT GCGCCAAGAT
    GTTAGCCGGT ACCAGAGGGT GGACGAGGCT CTCCCACCAA ACTGGGAGGC CCGCATTGAC
    AGCCACGGCA GGATCTTCTA CGTGGATCAC GTGAACAGAA CCACGACGTG GCAGCGGCCC
    ACCGCTCCCC CCGCCCCGCA GGTGCTGCAG AGGTCCAACT CCATACAGCA GATGGAGCAG
    CTGAACCGGC GATACCAGAG TATCCGCAGA ACCATGACTA ACGAGAGGCC CGAGGAAAAT
    AGCAGTGCTC TTGATGGGGC AGGAGAGGAG GCCGACTTTC ACCAAGCCAG TGCAGATTTC
    CGACGGGAGA GCGTCCTGCC CCACTCTACC TCCAGATCCA GGCTCACGTT GCTGTTACAG
    TCGCCCCCTG TGAAGTTCCT CATCAGCCCA GAGTTCTTCA CCGTGCTGCA TTCGAACCCT
    AGTGCCTACC GCATGTTTAC AAACAACACG TGTTTGAAGC ACATGATCAC CAAAGTCCGG
    CGGGACACCC ACCACTTTGA ACGCTACCAG CATAACCGGG ACCTTGTGGG ATTCCTCAAC
    ATGTTTGCAA ACAAACAACT GGAGCTGCCG AGGGGCTGGG AAATGAAACA CGATCATCAG
    GGCAAGGCAT TTTTTGTTGA CCACAACTCC CGGACCACCA CTTTCATTGA CCCCCGGCTC
    CCACTCCAGA GCAGCAGACC CACGAGTGCG CTGGTGCATC GACAGCATCT GACGAGGCAG
    CGCAGCCACA GTGCAGGCGA GGTGGGAGAA GATTCTCGAC ATGCCGGACC ACCAGTTCTT
    CCCAGGCCGT CGAGTACGTT CAACACAGTC AGCAGGCCGC AGTACCAGGA CATGGTTCCA
    GTGGCTTACA ATGACAAGAT TGTTGCATTT CTTCGCCAAC CCAACATCTT TGAAATTCTA
    CAGGAGCGTC AACCTGATCT TACCAGGAAT CACTCACTCA GGGAGAAGAT CCAATTTATC
    CGAACTGAAG GGACCCCAGG ATTGGTGCGT CTTTCAAGTG ATGCAGACCT TGTTATGTTG
    CTAAGTTTAT TTGAAGAAGA GATAATGTCA TACGTGCCTC CACATGCCTT ACTCCATCCC
    AGCTACTGTC AGTCCCCACG TGGCTCCCCT GTGTCCTCTC CCCAGAACTC GCCAGGTACT
    CAGCGTGCCA ACGCCCGGGC TCCCGCCCCT TACAAGCGAG ACTTTGAAGC CAAACTGAGG
    AATTTTTACA GGAAGTTAGA GACTAAAGGA TATGGACAAG GCCCAGGGAA ATTAAAGTTA
    ATTATCAGAA GAGATCACTT GCTCGAAGAT GCTTTTAATC AGATTATGGG CTATTCCAGA
    AAAGACCTGC AGAGAAATAA GCTGTACGTC ACATTTGTCG GGGAGGAAGG GCTGGATTAC
    AGTGGACCTT CCAGAGAGTT TTTCTTCCTG GTATCCAGAG AGCTCTTTAA CCCATATTAC
    GGCTTATTTG AATATTCAGC CAATGACACA TACACAGTAC AAATAAGTCC CATGTCTGCT
    TTTGTAGACA ATCACCATGA ATGGTTCCGG TTCAGTGGTA GGATCCTTGG TCTTGCGCTG
    ATTCACCAGT ATTTGTTGGA TGCCTTCTTC ACGCGGCCCT TCTATAAGGC TCTTCTCAGA
    ATTCTGTGTG ACCTGAGTGA TCTAGAATAC CTTGATGAGG AGTTCCACCA GAGCCTGCAG
    TGGATGAAAG ACAATGATAT CCATGACATT CTGGACCTCA CGTTCACAGT GAACGAAGAA
    GTTTTCGGGC AGATAACTGA ACGAGAATTA AAACCAGGGG GTGCCAACAT CCCAGTCACA
    GAGAAGAACA AGAAGGAGTA CATCGAGAGG ATGGTGAAAT GGAGGATTGA GCGGGGTGTT
    GTGCAGCAAA CGGAGAGCTT AGTGCGTGGC TTCTACGAGG TGGTGGATGC CAGGCTAGTA
    TCTGTTTTTG ATGCAAGAGA ACTGGAACTG GTCATCGCAG GCACAGCTGA AATAGACCTA
    AGTGATTGGA GAAACAACAC AGAATACAGA GGAGGTTATC ATGACAATCA TATTGTAATT
    CGGTGGTTCT GGGCTGCAGT GGAGAGATTC AACAATGAAC AACGATTAAG GTTGTTACAG
    TTTGTCACAG GCACATCCAG CATTCCCTAT GAAGGATTTG CTTCTCTCCG AGGGAGTAAT
    GGCCCAAGAA GATTCTGCGT GGAGAAATGG GGGAAAATCA CCGCTCTTCC CAGAGCTCAC
    ACTTGTTTTA ACCGTCTGGA TCTCCCTCCA TACCCATCCT TCTCCATGCT TTATGAAAAA
    CTTTTGACAG CAGTTGAGGA GACCAGTACC TTTGGACTTG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020930200.1
    CDS182..4906
    Translation

    Target ORF information:

    RefSeq Version XM_021074541.1
    Organism Sus scrofa(pig)
    Definition Sus scrofa HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021074541.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCTAGCT CAGCCCGGGA GCACCTGCTT TTTGTGCGGC GGCGAAATCC CCAGATGCGG 
    TACACTCTGA GTCCAGAGAA CCTCCAGAGC CTGGCTGCCC AGAGCTCCAT GCCTGAGAAC
    ATGACCCTGC AGCGGGCCAA CAGCGACACA GATCTGGTGA CTTCTGAGAG CCGGTCCAGC
    CTGACTGCCA GCATGTATGA GTACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG
    GACATTAAAG AGGAGGTGGA CCCGAGTGAT TGGATTGGAC TTTATCACAT AGATGAGAAT
    TCTCCAGCCA ACTTCTGGGA TTCTAAAAAC AGGGGTGTGA CTGGAACACA AAAAGGACAA
    ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAGC CGGAGATAAA AATCTGTTTC
    AAATACTACC ACGGCATTAG TGGAGCCCTG CGAGCCACGA CCCCCTGCAT CACGGTGAAG
    AACCCTGCTG TGATGATGGG GGCAGAAGGC ATGGAAGGAG GTGCTGCAGG CAGTCAGCAT
    TCTCGGAAAC TCGTTAGCTT TACGTTGTCA GATCTTAGGG CAGTTGGGCT AAAGAAAGGG
    ATGTTCTTCA ATCCTGACCC TTATCTGAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT
    TTCCCCACCT GTGCCCACCA TGGGCAGGAG AGGAGGTCTA CCATCATCAG TAACACCACT
    AATCCAATCT GGCACCGAGA GAAATACTCC TTTTTTGCAC TCCTTACTGA TGTCTTAGAA
    ATTGAAATTA AAGACAAATT TGCCAAGAGC CGTCCTATCA TCAAGCGTTT TCTGGGGAAA
    CTAACCATCC CAGTCCAGAG GCTGTTGGAG CGACAAGCCA TCGGTGATCA GATGCTCAGC
    TACAACCTTG GCAGAAGGCT CCCAGCTGAC CACGTGAGTG GGTACCTCCA GTTCAGAGTG
    GAGGTCACGT CTTCTGTGCA TGAAGATGCT TCTCCAGAAG CAGTTGGCAC AATACTTGGA
    GTCAATACTG TGAATGGAGA CCTAGGAAGC CCTTCTGATG AGGAGGACAT GCCAGGGGGC
    CACCACGACA GCCCGGTTTG TTCTAATGGA CCAGTTTCTG AGGAAAGTGC TGATGGGACC
    CCCAAGCATT CTTTCAGGAC TAGCTCTACT CTGGAAATAG ACACGGAAGA TTTAACCTCT
    ACTTCTTCAA GGACCTCACC TCCCAGAGGA CGTCAGGATT CCCTCAACGA TTATTTAGAT
    GCTATTGAAC ACAATGGCCA TTCCAGGTCA GGTGCAGCTA CCTGCTCAGA GAGGTCCATG
    GGAGCCTCTC CAAAACTAAG GAGTAGTTTT CCCACCGACA CGAGACTCAA TGCCATGCTT
    CATATTGATT CAGATGAGGA AGACCACGAG TTCCAGCAGG ACCTGGGTTA TCCATCTTCC
    TTGGAGGAGG AAGGAGGGTT GATAATGTTT AGCAGGGCAT CAAGAACTGA TGACGGGAGC
    CTCACATCTC AGACAAAGCC GGAAGACATC CCAGTGGAGA CTGAGGAAGC CTCTATCCAC
    GAACCTGCTT CCTTTGAGGA TAAGCCAGAA CATCTTCCGG AGCTTACAGA GAGCTCCTTA
    CCCTCAGGCC CCATGCCAGA TGAAAGTGAA AATGGCCTGG AGTCTCAACC AGGTGCCGAC
    CAGGGCAGCA CTGAATTGTG TGGCTCTCAG GAGGTCGATC AGCCTACGAG TGGGGCAGAC
    ACGGGCACTT CTGACACGTC GGGAGGAAGC AGAAGAGCCA TCAGTGAGAC AGAGTCCCTT
    GATCAGGGGT CCGAGCCTTC CCAGGTGTCC TCTGAAACAG AACCCAGTGA CCCTGCCAGG
    ACAGAGAGCG TGAGTGAGGC CAGCACCAGG CCTGAGGGAG AGAGTGACCT GGAAGGGGCC
    GACAGCTCCT GCAATGAGAG TGTCACCACG CAGCTGTCCT CGGTGGAGAC ACGGTGCTCA
    TCTCTTGAGA GTGCACGGTT TCCCGAAACC CCAGCCTTTT CTTCTCAGGA GGAGGAAGAT
    GGAGCCTGTG CAGCAGAGCC CACCAGCAGT GGCCCTGCCG AGGGGTCACA GGATTCCATA
    TGCACTCCTG GGTCCTTACC TGTGGTGCAG GTGCCCAGTG GAGAGGAGGA AGGGCCAGGG
    GCAGAATCGG CCACCTTACC TGACCAGGAG GAGGTGGGGG AGGTCTGGCA GCGGAGGGGG
    AGCCTGGAGG GAGCTGCTGC TGCTGCTGCT GCTGCTGAGA GCCAGCCCCA AGAAGAGGGT
    GATGCTGGGG AAGCCCAAGG GGCTTGTGAA GGGGCCACTG CCCAGGAGGA GGGTGCTACT
    GGAGGTTCTC AAGCCAACGG CCACCAGCCA CTGCGATCTC TGCCTTCAGT GCGCCAAGAT
    GTTAGCCGGT ACCAGAGGGT GGACGAGGCT CTCCCACCAA ACTGGGAGGC CCGCATTGAC
    AGCCACGGCA GGATCTTCTA CGTGGATCAC GTGAACAGAA CCACGACGTG GCAGCGGCCC
    ACCGCTCCCC CCGCCCCGCA GGTGCTGCAG AGGTCCAACT CCATACAGCA GATGGAGCAG
    CTGAACCGGC GATACCAGAG TATCCGCAGA ACCATGACTA ACGAGAGGCC CGAGGAAAAT
    AGCAGTGCTC TTGATGGGGC AGGAGAGGAG GCCGACTTTC ACCAAGCCAG TGCAGATTTC
    CGACGGGAGA GCGTCCTGCC CCACTCTACC TCCAGATCCA GGCTCACGTT GCTGTTACAG
    TCGCCCCCTG TGAAGTTCCT CATCAGCCCA GAGTTCTTCA CCGTGCTGCA TTCGAACCCT
    AGTGCCTACC GCATGTTTAC AAACAACACG TGTTTGAAGC ACATGATCAC CAAAGTCCGG
    CGGGACACCC ACCACTTTGA ACGCTACCAG CATAACCGGG ACCTTGTGGG ATTCCTCAAC
    ATGTTTGCAA ACAAACAACT GGAGCTGCCG AGGGGCTGGG AAATGAAACA CGATCATCAG
    GGCAAGGCAT TTTTTGTTGA CCACAACTCC CGGACCACCA CTTTCATTGA CCCCCGGCTC
    CCACTCCAGA GCAGCAGACC CACGAGTGCG CTGGTGCATC GACAGCATCT GACGAGGCAG
    CGCAGCCACA GTGCAGGCGA GGTGGGAGAA GATTCTCGAC ATGCCGGACC ACCAGTTCTT
    CCCAGGCCGT CGAGTACGTT CAACACAGTC AGCAGGCCGC AGTACCAGGA CATGGTTCCA
    GTGGCTTACA ATGACAAGAT TGTTGCATTT CTTCGCCAAC CCAACATCTT TGAAATTCTA
    CAGGAGCGTC AACCTGATCT TACCAGGAAT CACTCACTCA GGGAGAAGAT CCAATTTATC
    CGAACTGAAG GGACCCCAGG ATTGGTGCGT CTTTCAAGTG ATGCAGACCT TGTTATGTTG
    CTAAGTTTAT TTGAAGAAGA GATAATGTCA TACGTGCCTC CACATGCCTT ACTCCATCCC
    AGCTACTGTC AGTCCCCACG TGGCTCCCCT GTGTCCTCTC CCCAGAACTC GCCAGGTACT
    CAGCGTGCCA ACGCCCGGGC TCCCGCCCCT TACAAGCGAG ACTTTGAAGC CAAACTGAGG
    AATTTTTACA GGAAGTTAGA GACTAAAGGA TATGGACAAG GCCCAGGGAA ATTAAAGTTA
    ATTATCAGAA GAGATCACTT GCTCGAAGAT GCTTTTAATC AGATTATGGG CTATTCCAGA
    AAAGACCTGC AGAGAAATAA GCTGTACGTC ACATTTGTCG GGGAGGAAGG GCTGGATTAC
    AGTGGACCTT CCAGAGAGTT TTTCTTCCTG GTATCCAGAG AGCTCTTTAA CCCATATTAC
    GGCTTATTTG AATATTCAGC CAATGACACA TACACAGTAC AAATAAGTCC CATGTCTGCT
    TTTGTAGACA ATCACCATGA ATGGTTCCGG TTCAGTGGTA GGATCCTTGG TCTTGCGCTG
    ATTCACCAGT ATTTGTTGGA TGCCTTCTTC ACGCGGCCCT TCTATAAGGC TCTTCTCAGA
    ATTCTGTGTG ACCTGAGTGA TCTAGAATAC CTTGATGAGG AGTTCCACCA GAGCCTGCAG
    TGGATGAAAG ACAATGATAT CCATGACATT CTGGACCTCA CGTTCACAGT GAACGAAGAA
    GTTTTCGGGC AGATAACTGA ACGAGAATTA AAACCAGGGG GTGCCAACAT CCCAGTCACA
    GAGAAGAACA AGAAGGAGTA CATCGAGAGG ATGGTGAAAT GGAGGATTGA GCGGGGTGTT
    GTGCAGCAAA CGGAGAGCTT AGTGCGTGGC TTCTACGAGG TGGTGGATGC CAGGCTAGTA
    TCTGTTTTTG ATGCAAGAGA ACTGGAACTG GTCATCGCAG GCACAGCTGA AATAGACCTA
    AGTGATTGGA GAAACAACAC AGAATACAGA GGAGGTTATC ATGACAATCA TATTGTAATT
    CGGTGGTTCT GGGCTGCAGT GGAGAGATTC AACAATGAAC AACGATTAAG GTTGTTACAG
    TTTGTCACAG GCACATCCAG CATTCCCTAT GAAGGATTTG CTTCTCTCCG AGGGAGTAAT
    GGCCCAAGAA GATTCTGCGT GGAGAAATGG GGGAAAATCA CCGCTCTTCC CAGAGCTCAC
    ACTTGTTTTA ACCGTCTGGA TCTCCCTCCA TACCCATCCT TCTCCATGCT TTATGAAAAA
    CTTTTGACAG CAGTTGAGGA GACCAGTACC TTTGGACTTG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe01255
    Clone ID Related Accession (Same CDS sequence) XM_021074541.1 , XM_021074543.1 , NM_001198924.1 , XM_021074542.1 , XM_021074545.1 , XM_021074544.1
    Accession Version XM_021074543.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4725bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product E3 ubiquitin-protein ligase HECW2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010457.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCTAGCT CAGCCCGGGA GCACCTGCTT TTTGTGCGGC GGCGAAATCC CCAGATGCGG 
    TACACTCTGA GTCCAGAGAA CCTCCAGAGC CTGGCTGCCC AGAGCTCCAT GCCTGAGAAC
    ATGACCCTGC AGCGGGCCAA CAGCGACACA GATCTGGTGA CTTCTGAGAG CCGGTCCAGC
    CTGACTGCCA GCATGTATGA GTACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG
    GACATTAAAG AGGAGGTGGA CCCGAGTGAT TGGATTGGAC TTTATCACAT AGATGAGAAT
    TCTCCAGCCA ACTTCTGGGA TTCTAAAAAC AGGGGTGTGA CTGGAACACA AAAAGGACAA
    ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAGC CGGAGATAAA AATCTGTTTC
    AAATACTACC ACGGCATTAG TGGAGCCCTG CGAGCCACGA CCCCCTGCAT CACGGTGAAG
    AACCCTGCTG TGATGATGGG GGCAGAAGGC ATGGAAGGAG GTGCTGCAGG CAGTCAGCAT
    TCTCGGAAAC TCGTTAGCTT TACGTTGTCA GATCTTAGGG CAGTTGGGCT AAAGAAAGGG
    ATGTTCTTCA ATCCTGACCC TTATCTGAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT
    TTCCCCACCT GTGCCCACCA TGGGCAGGAG AGGAGGTCTA CCATCATCAG TAACACCACT
    AATCCAATCT GGCACCGAGA GAAATACTCC TTTTTTGCAC TCCTTACTGA TGTCTTAGAA
    ATTGAAATTA AAGACAAATT TGCCAAGAGC CGTCCTATCA TCAAGCGTTT TCTGGGGAAA
    CTAACCATCC CAGTCCAGAG GCTGTTGGAG CGACAAGCCA TCGGTGATCA GATGCTCAGC
    TACAACCTTG GCAGAAGGCT CCCAGCTGAC CACGTGAGTG GGTACCTCCA GTTCAGAGTG
    GAGGTCACGT CTTCTGTGCA TGAAGATGCT TCTCCAGAAG CAGTTGGCAC AATACTTGGA
    GTCAATACTG TGAATGGAGA CCTAGGAAGC CCTTCTGATG AGGAGGACAT GCCAGGGGGC
    CACCACGACA GCCCGGTTTG TTCTAATGGA CCAGTTTCTG AGGAAAGTGC TGATGGGACC
    CCCAAGCATT CTTTCAGGAC TAGCTCTACT CTGGAAATAG ACACGGAAGA TTTAACCTCT
    ACTTCTTCAA GGACCTCACC TCCCAGAGGA CGTCAGGATT CCCTCAACGA TTATTTAGAT
    GCTATTGAAC ACAATGGCCA TTCCAGGTCA GGTGCAGCTA CCTGCTCAGA GAGGTCCATG
    GGAGCCTCTC CAAAACTAAG GAGTAGTTTT CCCACCGACA CGAGACTCAA TGCCATGCTT
    CATATTGATT CAGATGAGGA AGACCACGAG TTCCAGCAGG ACCTGGGTTA TCCATCTTCC
    TTGGAGGAGG AAGGAGGGTT GATAATGTTT AGCAGGGCAT CAAGAACTGA TGACGGGAGC
    CTCACATCTC AGACAAAGCC GGAAGACATC CCAGTGGAGA CTGAGGAAGC CTCTATCCAC
    GAACCTGCTT CCTTTGAGGA TAAGCCAGAA CATCTTCCGG AGCTTACAGA GAGCTCCTTA
    CCCTCAGGCC CCATGCCAGA TGAAAGTGAA AATGGCCTGG AGTCTCAACC AGGTGCCGAC
    CAGGGCAGCA CTGAATTGTG TGGCTCTCAG GAGGTCGATC AGCCTACGAG TGGGGCAGAC
    ACGGGCACTT CTGACACGTC GGGAGGAAGC AGAAGAGCCA TCAGTGAGAC AGAGTCCCTT
    GATCAGGGGT CCGAGCCTTC CCAGGTGTCC TCTGAAACAG AACCCAGTGA CCCTGCCAGG
    ACAGAGAGCG TGAGTGAGGC CAGCACCAGG CCTGAGGGAG AGAGTGACCT GGAAGGGGCC
    GACAGCTCCT GCAATGAGAG TGTCACCACG CAGCTGTCCT CGGTGGAGAC ACGGTGCTCA
    TCTCTTGAGA GTGCACGGTT TCCCGAAACC CCAGCCTTTT CTTCTCAGGA GGAGGAAGAT
    GGAGCCTGTG CAGCAGAGCC CACCAGCAGT GGCCCTGCCG AGGGGTCACA GGATTCCATA
    TGCACTCCTG GGTCCTTACC TGTGGTGCAG GTGCCCAGTG GAGAGGAGGA AGGGCCAGGG
    GCAGAATCGG CCACCTTACC TGACCAGGAG GAGGTGGGGG AGGTCTGGCA GCGGAGGGGG
    AGCCTGGAGG GAGCTGCTGC TGCTGCTGCT GCTGCTGAGA GCCAGCCCCA AGAAGAGGGT
    GATGCTGGGG AAGCCCAAGG GGCTTGTGAA GGGGCCACTG CCCAGGAGGA GGGTGCTACT
    GGAGGTTCTC AAGCCAACGG CCACCAGCCA CTGCGATCTC TGCCTTCAGT GCGCCAAGAT
    GTTAGCCGGT ACCAGAGGGT GGACGAGGCT CTCCCACCAA ACTGGGAGGC CCGCATTGAC
    AGCCACGGCA GGATCTTCTA CGTGGATCAC GTGAACAGAA CCACGACGTG GCAGCGGCCC
    ACCGCTCCCC CCGCCCCGCA GGTGCTGCAG AGGTCCAACT CCATACAGCA GATGGAGCAG
    CTGAACCGGC GATACCAGAG TATCCGCAGA ACCATGACTA ACGAGAGGCC CGAGGAAAAT
    AGCAGTGCTC TTGATGGGGC AGGAGAGGAG GCCGACTTTC ACCAAGCCAG TGCAGATTTC
    CGACGGGAGA GCGTCCTGCC CCACTCTACC TCCAGATCCA GGCTCACGTT GCTGTTACAG
    TCGCCCCCTG TGAAGTTCCT CATCAGCCCA GAGTTCTTCA CCGTGCTGCA TTCGAACCCT
    AGTGCCTACC GCATGTTTAC AAACAACACG TGTTTGAAGC ACATGATCAC CAAAGTCCGG
    CGGGACACCC ACCACTTTGA ACGCTACCAG CATAACCGGG ACCTTGTGGG ATTCCTCAAC
    ATGTTTGCAA ACAAACAACT GGAGCTGCCG AGGGGCTGGG AAATGAAACA CGATCATCAG
    GGCAAGGCAT TTTTTGTTGA CCACAACTCC CGGACCACCA CTTTCATTGA CCCCCGGCTC
    CCACTCCAGA GCAGCAGACC CACGAGTGCG CTGGTGCATC GACAGCATCT GACGAGGCAG
    CGCAGCCACA GTGCAGGCGA GGTGGGAGAA GATTCTCGAC ATGCCGGACC ACCAGTTCTT
    CCCAGGCCGT CGAGTACGTT CAACACAGTC AGCAGGCCGC AGTACCAGGA CATGGTTCCA
    GTGGCTTACA ATGACAAGAT TGTTGCATTT CTTCGCCAAC CCAACATCTT TGAAATTCTA
    CAGGAGCGTC AACCTGATCT TACCAGGAAT CACTCACTCA GGGAGAAGAT CCAATTTATC
    CGAACTGAAG GGACCCCAGG ATTGGTGCGT CTTTCAAGTG ATGCAGACCT TGTTATGTTG
    CTAAGTTTAT TTGAAGAAGA GATAATGTCA TACGTGCCTC CACATGCCTT ACTCCATCCC
    AGCTACTGTC AGTCCCCACG TGGCTCCCCT GTGTCCTCTC CCCAGAACTC GCCAGGTACT
    CAGCGTGCCA ACGCCCGGGC TCCCGCCCCT TACAAGCGAG ACTTTGAAGC CAAACTGAGG
    AATTTTTACA GGAAGTTAGA GACTAAAGGA TATGGACAAG GCCCAGGGAA ATTAAAGTTA
    ATTATCAGAA GAGATCACTT GCTCGAAGAT GCTTTTAATC AGATTATGGG CTATTCCAGA
    AAAGACCTGC AGAGAAATAA GCTGTACGTC ACATTTGTCG GGGAGGAAGG GCTGGATTAC
    AGTGGACCTT CCAGAGAGTT TTTCTTCCTG GTATCCAGAG AGCTCTTTAA CCCATATTAC
    GGCTTATTTG AATATTCAGC CAATGACACA TACACAGTAC AAATAAGTCC CATGTCTGCT
    TTTGTAGACA ATCACCATGA ATGGTTCCGG TTCAGTGGTA GGATCCTTGG TCTTGCGCTG
    ATTCACCAGT ATTTGTTGGA TGCCTTCTTC ACGCGGCCCT TCTATAAGGC TCTTCTCAGA
    ATTCTGTGTG ACCTGAGTGA TCTAGAATAC CTTGATGAGG AGTTCCACCA GAGCCTGCAG
    TGGATGAAAG ACAATGATAT CCATGACATT CTGGACCTCA CGTTCACAGT GAACGAAGAA
    GTTTTCGGGC AGATAACTGA ACGAGAATTA AAACCAGGGG GTGCCAACAT CCCAGTCACA
    GAGAAGAACA AGAAGGAGTA CATCGAGAGG ATGGTGAAAT GGAGGATTGA GCGGGGTGTT
    GTGCAGCAAA CGGAGAGCTT AGTGCGTGGC TTCTACGAGG TGGTGGATGC CAGGCTAGTA
    TCTGTTTTTG ATGCAAGAGA ACTGGAACTG GTCATCGCAG GCACAGCTGA AATAGACCTA
    AGTGATTGGA GAAACAACAC AGAATACAGA GGAGGTTATC ATGACAATCA TATTGTAATT
    CGGTGGTTCT GGGCTGCAGT GGAGAGATTC AACAATGAAC AACGATTAAG GTTGTTACAG
    TTTGTCACAG GCACATCCAG CATTCCCTAT GAAGGATTTG CTTCTCTCCG AGGGAGTAAT
    GGCCCAAGAA GATTCTGCGT GGAGAAATGG GGGAAAATCA CCGCTCTTCC CAGAGCTCAC
    ACTTGTTTTA ACCGTCTGGA TCTCCCTCCA TACCCATCCT TCTCCATGCT TTATGAAAAA
    CTTTTGACAG CAGTTGAGGA GACCAGTACC TTTGGACTTG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020930202.1
    CDS158..4882
    Translation

    Target ORF information:

    RefSeq Version XM_021074543.1
    Organism Sus scrofa(pig)
    Definition Sus scrofa HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021074543.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCTAGCT CAGCCCGGGA GCACCTGCTT TTTGTGCGGC GGCGAAATCC CCAGATGCGG 
    TACACTCTGA GTCCAGAGAA CCTCCAGAGC CTGGCTGCCC AGAGCTCCAT GCCTGAGAAC
    ATGACCCTGC AGCGGGCCAA CAGCGACACA GATCTGGTGA CTTCTGAGAG CCGGTCCAGC
    CTGACTGCCA GCATGTATGA GTACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG
    GACATTAAAG AGGAGGTGGA CCCGAGTGAT TGGATTGGAC TTTATCACAT AGATGAGAAT
    TCTCCAGCCA ACTTCTGGGA TTCTAAAAAC AGGGGTGTGA CTGGAACACA AAAAGGACAA
    ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAGC CGGAGATAAA AATCTGTTTC
    AAATACTACC ACGGCATTAG TGGAGCCCTG CGAGCCACGA CCCCCTGCAT CACGGTGAAG
    AACCCTGCTG TGATGATGGG GGCAGAAGGC ATGGAAGGAG GTGCTGCAGG CAGTCAGCAT
    TCTCGGAAAC TCGTTAGCTT TACGTTGTCA GATCTTAGGG CAGTTGGGCT AAAGAAAGGG
    ATGTTCTTCA ATCCTGACCC TTATCTGAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT
    TTCCCCACCT GTGCCCACCA TGGGCAGGAG AGGAGGTCTA CCATCATCAG TAACACCACT
    AATCCAATCT GGCACCGAGA GAAATACTCC TTTTTTGCAC TCCTTACTGA TGTCTTAGAA
    ATTGAAATTA AAGACAAATT TGCCAAGAGC CGTCCTATCA TCAAGCGTTT TCTGGGGAAA
    CTAACCATCC CAGTCCAGAG GCTGTTGGAG CGACAAGCCA TCGGTGATCA GATGCTCAGC
    TACAACCTTG GCAGAAGGCT CCCAGCTGAC CACGTGAGTG GGTACCTCCA GTTCAGAGTG
    GAGGTCACGT CTTCTGTGCA TGAAGATGCT TCTCCAGAAG CAGTTGGCAC AATACTTGGA
    GTCAATACTG TGAATGGAGA CCTAGGAAGC CCTTCTGATG AGGAGGACAT GCCAGGGGGC
    CACCACGACA GCCCGGTTTG TTCTAATGGA CCAGTTTCTG AGGAAAGTGC TGATGGGACC
    CCCAAGCATT CTTTCAGGAC TAGCTCTACT CTGGAAATAG ACACGGAAGA TTTAACCTCT
    ACTTCTTCAA GGACCTCACC TCCCAGAGGA CGTCAGGATT CCCTCAACGA TTATTTAGAT
    GCTATTGAAC ACAATGGCCA TTCCAGGTCA GGTGCAGCTA CCTGCTCAGA GAGGTCCATG
    GGAGCCTCTC CAAAACTAAG GAGTAGTTTT CCCACCGACA CGAGACTCAA TGCCATGCTT
    CATATTGATT CAGATGAGGA AGACCACGAG TTCCAGCAGG ACCTGGGTTA TCCATCTTCC
    TTGGAGGAGG AAGGAGGGTT GATAATGTTT AGCAGGGCAT CAAGAACTGA TGACGGGAGC
    CTCACATCTC AGACAAAGCC GGAAGACATC CCAGTGGAGA CTGAGGAAGC CTCTATCCAC
    GAACCTGCTT CCTTTGAGGA TAAGCCAGAA CATCTTCCGG AGCTTACAGA GAGCTCCTTA
    CCCTCAGGCC CCATGCCAGA TGAAAGTGAA AATGGCCTGG AGTCTCAACC AGGTGCCGAC
    CAGGGCAGCA CTGAATTGTG TGGCTCTCAG GAGGTCGATC AGCCTACGAG TGGGGCAGAC
    ACGGGCACTT CTGACACGTC GGGAGGAAGC AGAAGAGCCA TCAGTGAGAC AGAGTCCCTT
    GATCAGGGGT CCGAGCCTTC CCAGGTGTCC TCTGAAACAG AACCCAGTGA CCCTGCCAGG
    ACAGAGAGCG TGAGTGAGGC CAGCACCAGG CCTGAGGGAG AGAGTGACCT GGAAGGGGCC
    GACAGCTCCT GCAATGAGAG TGTCACCACG CAGCTGTCCT CGGTGGAGAC ACGGTGCTCA
    TCTCTTGAGA GTGCACGGTT TCCCGAAACC CCAGCCTTTT CTTCTCAGGA GGAGGAAGAT
    GGAGCCTGTG CAGCAGAGCC CACCAGCAGT GGCCCTGCCG AGGGGTCACA GGATTCCATA
    TGCACTCCTG GGTCCTTACC TGTGGTGCAG GTGCCCAGTG GAGAGGAGGA AGGGCCAGGG
    GCAGAATCGG CCACCTTACC TGACCAGGAG GAGGTGGGGG AGGTCTGGCA GCGGAGGGGG
    AGCCTGGAGG GAGCTGCTGC TGCTGCTGCT GCTGCTGAGA GCCAGCCCCA AGAAGAGGGT
    GATGCTGGGG AAGCCCAAGG GGCTTGTGAA GGGGCCACTG CCCAGGAGGA GGGTGCTACT
    GGAGGTTCTC AAGCCAACGG CCACCAGCCA CTGCGATCTC TGCCTTCAGT GCGCCAAGAT
    GTTAGCCGGT ACCAGAGGGT GGACGAGGCT CTCCCACCAA ACTGGGAGGC CCGCATTGAC
    AGCCACGGCA GGATCTTCTA CGTGGATCAC GTGAACAGAA CCACGACGTG GCAGCGGCCC
    ACCGCTCCCC CCGCCCCGCA GGTGCTGCAG AGGTCCAACT CCATACAGCA GATGGAGCAG
    CTGAACCGGC GATACCAGAG TATCCGCAGA ACCATGACTA ACGAGAGGCC CGAGGAAAAT
    AGCAGTGCTC TTGATGGGGC AGGAGAGGAG GCCGACTTTC ACCAAGCCAG TGCAGATTTC
    CGACGGGAGA GCGTCCTGCC CCACTCTACC TCCAGATCCA GGCTCACGTT GCTGTTACAG
    TCGCCCCCTG TGAAGTTCCT CATCAGCCCA GAGTTCTTCA CCGTGCTGCA TTCGAACCCT
    AGTGCCTACC GCATGTTTAC AAACAACACG TGTTTGAAGC ACATGATCAC CAAAGTCCGG
    CGGGACACCC ACCACTTTGA ACGCTACCAG CATAACCGGG ACCTTGTGGG ATTCCTCAAC
    ATGTTTGCAA ACAAACAACT GGAGCTGCCG AGGGGCTGGG AAATGAAACA CGATCATCAG
    GGCAAGGCAT TTTTTGTTGA CCACAACTCC CGGACCACCA CTTTCATTGA CCCCCGGCTC
    CCACTCCAGA GCAGCAGACC CACGAGTGCG CTGGTGCATC GACAGCATCT GACGAGGCAG
    CGCAGCCACA GTGCAGGCGA GGTGGGAGAA GATTCTCGAC ATGCCGGACC ACCAGTTCTT
    CCCAGGCCGT CGAGTACGTT CAACACAGTC AGCAGGCCGC AGTACCAGGA CATGGTTCCA
    GTGGCTTACA ATGACAAGAT TGTTGCATTT CTTCGCCAAC CCAACATCTT TGAAATTCTA
    CAGGAGCGTC AACCTGATCT TACCAGGAAT CACTCACTCA GGGAGAAGAT CCAATTTATC
    CGAACTGAAG GGACCCCAGG ATTGGTGCGT CTTTCAAGTG ATGCAGACCT TGTTATGTTG
    CTAAGTTTAT TTGAAGAAGA GATAATGTCA TACGTGCCTC CACATGCCTT ACTCCATCCC
    AGCTACTGTC AGTCCCCACG TGGCTCCCCT GTGTCCTCTC CCCAGAACTC GCCAGGTACT
    CAGCGTGCCA ACGCCCGGGC TCCCGCCCCT TACAAGCGAG ACTTTGAAGC CAAACTGAGG
    AATTTTTACA GGAAGTTAGA GACTAAAGGA TATGGACAAG GCCCAGGGAA ATTAAAGTTA
    ATTATCAGAA GAGATCACTT GCTCGAAGAT GCTTTTAATC AGATTATGGG CTATTCCAGA
    AAAGACCTGC AGAGAAATAA GCTGTACGTC ACATTTGTCG GGGAGGAAGG GCTGGATTAC
    AGTGGACCTT CCAGAGAGTT TTTCTTCCTG GTATCCAGAG AGCTCTTTAA CCCATATTAC
    GGCTTATTTG AATATTCAGC CAATGACACA TACACAGTAC AAATAAGTCC CATGTCTGCT
    TTTGTAGACA ATCACCATGA ATGGTTCCGG TTCAGTGGTA GGATCCTTGG TCTTGCGCTG
    ATTCACCAGT ATTTGTTGGA TGCCTTCTTC ACGCGGCCCT TCTATAAGGC TCTTCTCAGA
    ATTCTGTGTG ACCTGAGTGA TCTAGAATAC CTTGATGAGG AGTTCCACCA GAGCCTGCAG
    TGGATGAAAG ACAATGATAT CCATGACATT CTGGACCTCA CGTTCACAGT GAACGAAGAA
    GTTTTCGGGC AGATAACTGA ACGAGAATTA AAACCAGGGG GTGCCAACAT CCCAGTCACA
    GAGAAGAACA AGAAGGAGTA CATCGAGAGG ATGGTGAAAT GGAGGATTGA GCGGGGTGTT
    GTGCAGCAAA CGGAGAGCTT AGTGCGTGGC TTCTACGAGG TGGTGGATGC CAGGCTAGTA
    TCTGTTTTTG ATGCAAGAGA ACTGGAACTG GTCATCGCAG GCACAGCTGA AATAGACCTA
    AGTGATTGGA GAAACAACAC AGAATACAGA GGAGGTTATC ATGACAATCA TATTGTAATT
    CGGTGGTTCT GGGCTGCAGT GGAGAGATTC AACAATGAAC AACGATTAAG GTTGTTACAG
    TTTGTCACAG GCACATCCAG CATTCCCTAT GAAGGATTTG CTTCTCTCCG AGGGAGTAAT
    GGCCCAAGAA GATTCTGCGT GGAGAAATGG GGGAAAATCA CCGCTCTTCC CAGAGCTCAC
    ACTTGTTTTA ACCGTCTGGA TCTCCCTCCA TACCCATCCT TCTCCATGCT TTATGAAAAA
    CTTTTGACAG CAGTTGAGGA GACCAGTACC TTTGGACTTG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe01255
    Clone ID Related Accession (Same CDS sequence) XM_021074541.1 , XM_021074543.1 , NM_001198924.1 , XM_021074542.1 , XM_021074545.1 , XM_021074544.1
    Accession Version XM_021074542.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4725bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product E3 ubiquitin-protein ligase HECW2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010457.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCTAGCT CAGCCCGGGA GCACCTGCTT TTTGTGCGGC GGCGAAATCC CCAGATGCGG 
    TACACTCTGA GTCCAGAGAA CCTCCAGAGC CTGGCTGCCC AGAGCTCCAT GCCTGAGAAC
    ATGACCCTGC AGCGGGCCAA CAGCGACACA GATCTGGTGA CTTCTGAGAG CCGGTCCAGC
    CTGACTGCCA GCATGTATGA GTACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG
    GACATTAAAG AGGAGGTGGA CCCGAGTGAT TGGATTGGAC TTTATCACAT AGATGAGAAT
    TCTCCAGCCA ACTTCTGGGA TTCTAAAAAC AGGGGTGTGA CTGGAACACA AAAAGGACAA
    ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAGC CGGAGATAAA AATCTGTTTC
    AAATACTACC ACGGCATTAG TGGAGCCCTG CGAGCCACGA CCCCCTGCAT CACGGTGAAG
    AACCCTGCTG TGATGATGGG GGCAGAAGGC ATGGAAGGAG GTGCTGCAGG CAGTCAGCAT
    TCTCGGAAAC TCGTTAGCTT TACGTTGTCA GATCTTAGGG CAGTTGGGCT AAAGAAAGGG
    ATGTTCTTCA ATCCTGACCC TTATCTGAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT
    TTCCCCACCT GTGCCCACCA TGGGCAGGAG AGGAGGTCTA CCATCATCAG TAACACCACT
    AATCCAATCT GGCACCGAGA GAAATACTCC TTTTTTGCAC TCCTTACTGA TGTCTTAGAA
    ATTGAAATTA AAGACAAATT TGCCAAGAGC CGTCCTATCA TCAAGCGTTT TCTGGGGAAA
    CTAACCATCC CAGTCCAGAG GCTGTTGGAG CGACAAGCCA TCGGTGATCA GATGCTCAGC
    TACAACCTTG GCAGAAGGCT CCCAGCTGAC CACGTGAGTG GGTACCTCCA GTTCAGAGTG
    GAGGTCACGT CTTCTGTGCA TGAAGATGCT TCTCCAGAAG CAGTTGGCAC AATACTTGGA
    GTCAATACTG TGAATGGAGA CCTAGGAAGC CCTTCTGATG AGGAGGACAT GCCAGGGGGC
    CACCACGACA GCCCGGTTTG TTCTAATGGA CCAGTTTCTG AGGAAAGTGC TGATGGGACC
    CCCAAGCATT CTTTCAGGAC TAGCTCTACT CTGGAAATAG ACACGGAAGA TTTAACCTCT
    ACTTCTTCAA GGACCTCACC TCCCAGAGGA CGTCAGGATT CCCTCAACGA TTATTTAGAT
    GCTATTGAAC ACAATGGCCA TTCCAGGTCA GGTGCAGCTA CCTGCTCAGA GAGGTCCATG
    GGAGCCTCTC CAAAACTAAG GAGTAGTTTT CCCACCGACA CGAGACTCAA TGCCATGCTT
    CATATTGATT CAGATGAGGA AGACCACGAG TTCCAGCAGG ACCTGGGTTA TCCATCTTCC
    TTGGAGGAGG AAGGAGGGTT GATAATGTTT AGCAGGGCAT CAAGAACTGA TGACGGGAGC
    CTCACATCTC AGACAAAGCC GGAAGACATC CCAGTGGAGA CTGAGGAAGC CTCTATCCAC
    GAACCTGCTT CCTTTGAGGA TAAGCCAGAA CATCTTCCGG AGCTTACAGA GAGCTCCTTA
    CCCTCAGGCC CCATGCCAGA TGAAAGTGAA AATGGCCTGG AGTCTCAACC AGGTGCCGAC
    CAGGGCAGCA CTGAATTGTG TGGCTCTCAG GAGGTCGATC AGCCTACGAG TGGGGCAGAC
    ACGGGCACTT CTGACACGTC GGGAGGAAGC AGAAGAGCCA TCAGTGAGAC AGAGTCCCTT
    GATCAGGGGT CCGAGCCTTC CCAGGTGTCC TCTGAAACAG AACCCAGTGA CCCTGCCAGG
    ACAGAGAGCG TGAGTGAGGC CAGCACCAGG CCTGAGGGAG AGAGTGACCT GGAAGGGGCC
    GACAGCTCCT GCAATGAGAG TGTCACCACG CAGCTGTCCT CGGTGGAGAC ACGGTGCTCA
    TCTCTTGAGA GTGCACGGTT TCCCGAAACC CCAGCCTTTT CTTCTCAGGA GGAGGAAGAT
    GGAGCCTGTG CAGCAGAGCC CACCAGCAGT GGCCCTGCCG AGGGGTCACA GGATTCCATA
    TGCACTCCTG GGTCCTTACC TGTGGTGCAG GTGCCCAGTG GAGAGGAGGA AGGGCCAGGG
    GCAGAATCGG CCACCTTACC TGACCAGGAG GAGGTGGGGG AGGTCTGGCA GCGGAGGGGG
    AGCCTGGAGG GAGCTGCTGC TGCTGCTGCT GCTGCTGAGA GCCAGCCCCA AGAAGAGGGT
    GATGCTGGGG AAGCCCAAGG GGCTTGTGAA GGGGCCACTG CCCAGGAGGA GGGTGCTACT
    GGAGGTTCTC AAGCCAACGG CCACCAGCCA CTGCGATCTC TGCCTTCAGT GCGCCAAGAT
    GTTAGCCGGT ACCAGAGGGT GGACGAGGCT CTCCCACCAA ACTGGGAGGC CCGCATTGAC
    AGCCACGGCA GGATCTTCTA CGTGGATCAC GTGAACAGAA CCACGACGTG GCAGCGGCCC
    ACCGCTCCCC CCGCCCCGCA GGTGCTGCAG AGGTCCAACT CCATACAGCA GATGGAGCAG
    CTGAACCGGC GATACCAGAG TATCCGCAGA ACCATGACTA ACGAGAGGCC CGAGGAAAAT
    AGCAGTGCTC TTGATGGGGC AGGAGAGGAG GCCGACTTTC ACCAAGCCAG TGCAGATTTC
    CGACGGGAGA GCGTCCTGCC CCACTCTACC TCCAGATCCA GGCTCACGTT GCTGTTACAG
    TCGCCCCCTG TGAAGTTCCT CATCAGCCCA GAGTTCTTCA CCGTGCTGCA TTCGAACCCT
    AGTGCCTACC GCATGTTTAC AAACAACACG TGTTTGAAGC ACATGATCAC CAAAGTCCGG
    CGGGACACCC ACCACTTTGA ACGCTACCAG CATAACCGGG ACCTTGTGGG ATTCCTCAAC
    ATGTTTGCAA ACAAACAACT GGAGCTGCCG AGGGGCTGGG AAATGAAACA CGATCATCAG
    GGCAAGGCAT TTTTTGTTGA CCACAACTCC CGGACCACCA CTTTCATTGA CCCCCGGCTC
    CCACTCCAGA GCAGCAGACC CACGAGTGCG CTGGTGCATC GACAGCATCT GACGAGGCAG
    CGCAGCCACA GTGCAGGCGA GGTGGGAGAA GATTCTCGAC ATGCCGGACC ACCAGTTCTT
    CCCAGGCCGT CGAGTACGTT CAACACAGTC AGCAGGCCGC AGTACCAGGA CATGGTTCCA
    GTGGCTTACA ATGACAAGAT TGTTGCATTT CTTCGCCAAC CCAACATCTT TGAAATTCTA
    CAGGAGCGTC AACCTGATCT TACCAGGAAT CACTCACTCA GGGAGAAGAT CCAATTTATC
    CGAACTGAAG GGACCCCAGG ATTGGTGCGT CTTTCAAGTG ATGCAGACCT TGTTATGTTG
    CTAAGTTTAT TTGAAGAAGA GATAATGTCA TACGTGCCTC CACATGCCTT ACTCCATCCC
    AGCTACTGTC AGTCCCCACG TGGCTCCCCT GTGTCCTCTC CCCAGAACTC GCCAGGTACT
    CAGCGTGCCA ACGCCCGGGC TCCCGCCCCT TACAAGCGAG ACTTTGAAGC CAAACTGAGG
    AATTTTTACA GGAAGTTAGA GACTAAAGGA TATGGACAAG GCCCAGGGAA ATTAAAGTTA
    ATTATCAGAA GAGATCACTT GCTCGAAGAT GCTTTTAATC AGATTATGGG CTATTCCAGA
    AAAGACCTGC AGAGAAATAA GCTGTACGTC ACATTTGTCG GGGAGGAAGG GCTGGATTAC
    AGTGGACCTT CCAGAGAGTT TTTCTTCCTG GTATCCAGAG AGCTCTTTAA CCCATATTAC
    GGCTTATTTG AATATTCAGC CAATGACACA TACACAGTAC AAATAAGTCC CATGTCTGCT
    TTTGTAGACA ATCACCATGA ATGGTTCCGG TTCAGTGGTA GGATCCTTGG TCTTGCGCTG
    ATTCACCAGT ATTTGTTGGA TGCCTTCTTC ACGCGGCCCT TCTATAAGGC TCTTCTCAGA
    ATTCTGTGTG ACCTGAGTGA TCTAGAATAC CTTGATGAGG AGTTCCACCA GAGCCTGCAG
    TGGATGAAAG ACAATGATAT CCATGACATT CTGGACCTCA CGTTCACAGT GAACGAAGAA
    GTTTTCGGGC AGATAACTGA ACGAGAATTA AAACCAGGGG GTGCCAACAT CCCAGTCACA
    GAGAAGAACA AGAAGGAGTA CATCGAGAGG ATGGTGAAAT GGAGGATTGA GCGGGGTGTT
    GTGCAGCAAA CGGAGAGCTT AGTGCGTGGC TTCTACGAGG TGGTGGATGC CAGGCTAGTA
    TCTGTTTTTG ATGCAAGAGA ACTGGAACTG GTCATCGCAG GCACAGCTGA AATAGACCTA
    AGTGATTGGA GAAACAACAC AGAATACAGA GGAGGTTATC ATGACAATCA TATTGTAATT
    CGGTGGTTCT GGGCTGCAGT GGAGAGATTC AACAATGAAC AACGATTAAG GTTGTTACAG
    TTTGTCACAG GCACATCCAG CATTCCCTAT GAAGGATTTG CTTCTCTCCG AGGGAGTAAT
    GGCCCAAGAA GATTCTGCGT GGAGAAATGG GGGAAAATCA CCGCTCTTCC CAGAGCTCAC
    ACTTGTTTTA ACCGTCTGGA TCTCCCTCCA TACCCATCCT TCTCCATGCT TTATGAAAAA
    CTTTTGACAG CAGTTGAGGA GACCAGTACC TTTGGACTTG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020930201.1
    CDS139..4863
    Translation

    Target ORF information:

    RefSeq Version XM_021074542.1
    Organism Sus scrofa(pig)
    Definition Sus scrofa HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021074542.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCTAGCT CAGCCCGGGA GCACCTGCTT TTTGTGCGGC GGCGAAATCC CCAGATGCGG 
    TACACTCTGA GTCCAGAGAA CCTCCAGAGC CTGGCTGCCC AGAGCTCCAT GCCTGAGAAC
    ATGACCCTGC AGCGGGCCAA CAGCGACACA GATCTGGTGA CTTCTGAGAG CCGGTCCAGC
    CTGACTGCCA GCATGTATGA GTACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG
    GACATTAAAG AGGAGGTGGA CCCGAGTGAT TGGATTGGAC TTTATCACAT AGATGAGAAT
    TCTCCAGCCA ACTTCTGGGA TTCTAAAAAC AGGGGTGTGA CTGGAACACA AAAAGGACAA
    ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAGC CGGAGATAAA AATCTGTTTC
    AAATACTACC ACGGCATTAG TGGAGCCCTG CGAGCCACGA CCCCCTGCAT CACGGTGAAG
    AACCCTGCTG TGATGATGGG GGCAGAAGGC ATGGAAGGAG GTGCTGCAGG CAGTCAGCAT
    TCTCGGAAAC TCGTTAGCTT TACGTTGTCA GATCTTAGGG CAGTTGGGCT AAAGAAAGGG
    ATGTTCTTCA ATCCTGACCC TTATCTGAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT
    TTCCCCACCT GTGCCCACCA TGGGCAGGAG AGGAGGTCTA CCATCATCAG TAACACCACT
    AATCCAATCT GGCACCGAGA GAAATACTCC TTTTTTGCAC TCCTTACTGA TGTCTTAGAA
    ATTGAAATTA AAGACAAATT TGCCAAGAGC CGTCCTATCA TCAAGCGTTT TCTGGGGAAA
    CTAACCATCC CAGTCCAGAG GCTGTTGGAG CGACAAGCCA TCGGTGATCA GATGCTCAGC
    TACAACCTTG GCAGAAGGCT CCCAGCTGAC CACGTGAGTG GGTACCTCCA GTTCAGAGTG
    GAGGTCACGT CTTCTGTGCA TGAAGATGCT TCTCCAGAAG CAGTTGGCAC AATACTTGGA
    GTCAATACTG TGAATGGAGA CCTAGGAAGC CCTTCTGATG AGGAGGACAT GCCAGGGGGC
    CACCACGACA GCCCGGTTTG TTCTAATGGA CCAGTTTCTG AGGAAAGTGC TGATGGGACC
    CCCAAGCATT CTTTCAGGAC TAGCTCTACT CTGGAAATAG ACACGGAAGA TTTAACCTCT
    ACTTCTTCAA GGACCTCACC TCCCAGAGGA CGTCAGGATT CCCTCAACGA TTATTTAGAT
    GCTATTGAAC ACAATGGCCA TTCCAGGTCA GGTGCAGCTA CCTGCTCAGA GAGGTCCATG
    GGAGCCTCTC CAAAACTAAG GAGTAGTTTT CCCACCGACA CGAGACTCAA TGCCATGCTT
    CATATTGATT CAGATGAGGA AGACCACGAG TTCCAGCAGG ACCTGGGTTA TCCATCTTCC
    TTGGAGGAGG AAGGAGGGTT GATAATGTTT AGCAGGGCAT CAAGAACTGA TGACGGGAGC
    CTCACATCTC AGACAAAGCC GGAAGACATC CCAGTGGAGA CTGAGGAAGC CTCTATCCAC
    GAACCTGCTT CCTTTGAGGA TAAGCCAGAA CATCTTCCGG AGCTTACAGA GAGCTCCTTA
    CCCTCAGGCC CCATGCCAGA TGAAAGTGAA AATGGCCTGG AGTCTCAACC AGGTGCCGAC
    CAGGGCAGCA CTGAATTGTG TGGCTCTCAG GAGGTCGATC AGCCTACGAG TGGGGCAGAC
    ACGGGCACTT CTGACACGTC GGGAGGAAGC AGAAGAGCCA TCAGTGAGAC AGAGTCCCTT
    GATCAGGGGT CCGAGCCTTC CCAGGTGTCC TCTGAAACAG AACCCAGTGA CCCTGCCAGG
    ACAGAGAGCG TGAGTGAGGC CAGCACCAGG CCTGAGGGAG AGAGTGACCT GGAAGGGGCC
    GACAGCTCCT GCAATGAGAG TGTCACCACG CAGCTGTCCT CGGTGGAGAC ACGGTGCTCA
    TCTCTTGAGA GTGCACGGTT TCCCGAAACC CCAGCCTTTT CTTCTCAGGA GGAGGAAGAT
    GGAGCCTGTG CAGCAGAGCC CACCAGCAGT GGCCCTGCCG AGGGGTCACA GGATTCCATA
    TGCACTCCTG GGTCCTTACC TGTGGTGCAG GTGCCCAGTG GAGAGGAGGA AGGGCCAGGG
    GCAGAATCGG CCACCTTACC TGACCAGGAG GAGGTGGGGG AGGTCTGGCA GCGGAGGGGG
    AGCCTGGAGG GAGCTGCTGC TGCTGCTGCT GCTGCTGAGA GCCAGCCCCA AGAAGAGGGT
    GATGCTGGGG AAGCCCAAGG GGCTTGTGAA GGGGCCACTG CCCAGGAGGA GGGTGCTACT
    GGAGGTTCTC AAGCCAACGG CCACCAGCCA CTGCGATCTC TGCCTTCAGT GCGCCAAGAT
    GTTAGCCGGT ACCAGAGGGT GGACGAGGCT CTCCCACCAA ACTGGGAGGC CCGCATTGAC
    AGCCACGGCA GGATCTTCTA CGTGGATCAC GTGAACAGAA CCACGACGTG GCAGCGGCCC
    ACCGCTCCCC CCGCCCCGCA GGTGCTGCAG AGGTCCAACT CCATACAGCA GATGGAGCAG
    CTGAACCGGC GATACCAGAG TATCCGCAGA ACCATGACTA ACGAGAGGCC CGAGGAAAAT
    AGCAGTGCTC TTGATGGGGC AGGAGAGGAG GCCGACTTTC ACCAAGCCAG TGCAGATTTC
    CGACGGGAGA GCGTCCTGCC CCACTCTACC TCCAGATCCA GGCTCACGTT GCTGTTACAG
    TCGCCCCCTG TGAAGTTCCT CATCAGCCCA GAGTTCTTCA CCGTGCTGCA TTCGAACCCT
    AGTGCCTACC GCATGTTTAC AAACAACACG TGTTTGAAGC ACATGATCAC CAAAGTCCGG
    CGGGACACCC ACCACTTTGA ACGCTACCAG CATAACCGGG ACCTTGTGGG ATTCCTCAAC
    ATGTTTGCAA ACAAACAACT GGAGCTGCCG AGGGGCTGGG AAATGAAACA CGATCATCAG
    GGCAAGGCAT TTTTTGTTGA CCACAACTCC CGGACCACCA CTTTCATTGA CCCCCGGCTC
    CCACTCCAGA GCAGCAGACC CACGAGTGCG CTGGTGCATC GACAGCATCT GACGAGGCAG
    CGCAGCCACA GTGCAGGCGA GGTGGGAGAA GATTCTCGAC ATGCCGGACC ACCAGTTCTT
    CCCAGGCCGT CGAGTACGTT CAACACAGTC AGCAGGCCGC AGTACCAGGA CATGGTTCCA
    GTGGCTTACA ATGACAAGAT TGTTGCATTT CTTCGCCAAC CCAACATCTT TGAAATTCTA
    CAGGAGCGTC AACCTGATCT TACCAGGAAT CACTCACTCA GGGAGAAGAT CCAATTTATC
    CGAACTGAAG GGACCCCAGG ATTGGTGCGT CTTTCAAGTG ATGCAGACCT TGTTATGTTG
    CTAAGTTTAT TTGAAGAAGA GATAATGTCA TACGTGCCTC CACATGCCTT ACTCCATCCC
    AGCTACTGTC AGTCCCCACG TGGCTCCCCT GTGTCCTCTC CCCAGAACTC GCCAGGTACT
    CAGCGTGCCA ACGCCCGGGC TCCCGCCCCT TACAAGCGAG ACTTTGAAGC CAAACTGAGG
    AATTTTTACA GGAAGTTAGA GACTAAAGGA TATGGACAAG GCCCAGGGAA ATTAAAGTTA
    ATTATCAGAA GAGATCACTT GCTCGAAGAT GCTTTTAATC AGATTATGGG CTATTCCAGA
    AAAGACCTGC AGAGAAATAA GCTGTACGTC ACATTTGTCG GGGAGGAAGG GCTGGATTAC
    AGTGGACCTT CCAGAGAGTT TTTCTTCCTG GTATCCAGAG AGCTCTTTAA CCCATATTAC
    GGCTTATTTG AATATTCAGC CAATGACACA TACACAGTAC AAATAAGTCC CATGTCTGCT
    TTTGTAGACA ATCACCATGA ATGGTTCCGG TTCAGTGGTA GGATCCTTGG TCTTGCGCTG
    ATTCACCAGT ATTTGTTGGA TGCCTTCTTC ACGCGGCCCT TCTATAAGGC TCTTCTCAGA
    ATTCTGTGTG ACCTGAGTGA TCTAGAATAC CTTGATGAGG AGTTCCACCA GAGCCTGCAG
    TGGATGAAAG ACAATGATAT CCATGACATT CTGGACCTCA CGTTCACAGT GAACGAAGAA
    GTTTTCGGGC AGATAACTGA ACGAGAATTA AAACCAGGGG GTGCCAACAT CCCAGTCACA
    GAGAAGAACA AGAAGGAGTA CATCGAGAGG ATGGTGAAAT GGAGGATTGA GCGGGGTGTT
    GTGCAGCAAA CGGAGAGCTT AGTGCGTGGC TTCTACGAGG TGGTGGATGC CAGGCTAGTA
    TCTGTTTTTG ATGCAAGAGA ACTGGAACTG GTCATCGCAG GCACAGCTGA AATAGACCTA
    AGTGATTGGA GAAACAACAC AGAATACAGA GGAGGTTATC ATGACAATCA TATTGTAATT
    CGGTGGTTCT GGGCTGCAGT GGAGAGATTC AACAATGAAC AACGATTAAG GTTGTTACAG
    TTTGTCACAG GCACATCCAG CATTCCCTAT GAAGGATTTG CTTCTCTCCG AGGGAGTAAT
    GGCCCAAGAA GATTCTGCGT GGAGAAATGG GGGAAAATCA CCGCTCTTCC CAGAGCTCAC
    ACTTGTTTTA ACCGTCTGGA TCTCCCTCCA TACCCATCCT TCTCCATGCT TTATGAAAAA
    CTTTTGACAG CAGTTGAGGA GACCAGTACC TTTGGACTTG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe01255
    Clone ID Related Accession (Same CDS sequence) XM_021074541.1 , XM_021074543.1 , NM_001198924.1 , XM_021074542.1 , XM_021074545.1 , XM_021074544.1
    Accession Version XM_021074545.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4725bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product E3 ubiquitin-protein ligase HECW2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010457.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCTAGCT CAGCCCGGGA GCACCTGCTT TTTGTGCGGC GGCGAAATCC CCAGATGCGG 
    TACACTCTGA GTCCAGAGAA CCTCCAGAGC CTGGCTGCCC AGAGCTCCAT GCCTGAGAAC
    ATGACCCTGC AGCGGGCCAA CAGCGACACA GATCTGGTGA CTTCTGAGAG CCGGTCCAGC
    CTGACTGCCA GCATGTATGA GTACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG
    GACATTAAAG AGGAGGTGGA CCCGAGTGAT TGGATTGGAC TTTATCACAT AGATGAGAAT
    TCTCCAGCCA ACTTCTGGGA TTCTAAAAAC AGGGGTGTGA CTGGAACACA AAAAGGACAA
    ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAGC CGGAGATAAA AATCTGTTTC
    AAATACTACC ACGGCATTAG TGGAGCCCTG CGAGCCACGA CCCCCTGCAT CACGGTGAAG
    AACCCTGCTG TGATGATGGG GGCAGAAGGC ATGGAAGGAG GTGCTGCAGG CAGTCAGCAT
    TCTCGGAAAC TCGTTAGCTT TACGTTGTCA GATCTTAGGG CAGTTGGGCT AAAGAAAGGG
    ATGTTCTTCA ATCCTGACCC TTATCTGAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT
    TTCCCCACCT GTGCCCACCA TGGGCAGGAG AGGAGGTCTA CCATCATCAG TAACACCACT
    AATCCAATCT GGCACCGAGA GAAATACTCC TTTTTTGCAC TCCTTACTGA TGTCTTAGAA
    ATTGAAATTA AAGACAAATT TGCCAAGAGC CGTCCTATCA TCAAGCGTTT TCTGGGGAAA
    CTAACCATCC CAGTCCAGAG GCTGTTGGAG CGACAAGCCA TCGGTGATCA GATGCTCAGC
    TACAACCTTG GCAGAAGGCT CCCAGCTGAC CACGTGAGTG GGTACCTCCA GTTCAGAGTG
    GAGGTCACGT CTTCTGTGCA TGAAGATGCT TCTCCAGAAG CAGTTGGCAC AATACTTGGA
    GTCAATACTG TGAATGGAGA CCTAGGAAGC CCTTCTGATG AGGAGGACAT GCCAGGGGGC
    CACCACGACA GCCCGGTTTG TTCTAATGGA CCAGTTTCTG AGGAAAGTGC TGATGGGACC
    CCCAAGCATT CTTTCAGGAC TAGCTCTACT CTGGAAATAG ACACGGAAGA TTTAACCTCT
    ACTTCTTCAA GGACCTCACC TCCCAGAGGA CGTCAGGATT CCCTCAACGA TTATTTAGAT
    GCTATTGAAC ACAATGGCCA TTCCAGGTCA GGTGCAGCTA CCTGCTCAGA GAGGTCCATG
    GGAGCCTCTC CAAAACTAAG GAGTAGTTTT CCCACCGACA CGAGACTCAA TGCCATGCTT
    CATATTGATT CAGATGAGGA AGACCACGAG TTCCAGCAGG ACCTGGGTTA TCCATCTTCC
    TTGGAGGAGG AAGGAGGGTT GATAATGTTT AGCAGGGCAT CAAGAACTGA TGACGGGAGC
    CTCACATCTC AGACAAAGCC GGAAGACATC CCAGTGGAGA CTGAGGAAGC CTCTATCCAC
    GAACCTGCTT CCTTTGAGGA TAAGCCAGAA CATCTTCCGG AGCTTACAGA GAGCTCCTTA
    CCCTCAGGCC CCATGCCAGA TGAAAGTGAA AATGGCCTGG AGTCTCAACC AGGTGCCGAC
    CAGGGCAGCA CTGAATTGTG TGGCTCTCAG GAGGTCGATC AGCCTACGAG TGGGGCAGAC
    ACGGGCACTT CTGACACGTC GGGAGGAAGC AGAAGAGCCA TCAGTGAGAC AGAGTCCCTT
    GATCAGGGGT CCGAGCCTTC CCAGGTGTCC TCTGAAACAG AACCCAGTGA CCCTGCCAGG
    ACAGAGAGCG TGAGTGAGGC CAGCACCAGG CCTGAGGGAG AGAGTGACCT GGAAGGGGCC
    GACAGCTCCT GCAATGAGAG TGTCACCACG CAGCTGTCCT CGGTGGAGAC ACGGTGCTCA
    TCTCTTGAGA GTGCACGGTT TCCCGAAACC CCAGCCTTTT CTTCTCAGGA GGAGGAAGAT
    GGAGCCTGTG CAGCAGAGCC CACCAGCAGT GGCCCTGCCG AGGGGTCACA GGATTCCATA
    TGCACTCCTG GGTCCTTACC TGTGGTGCAG GTGCCCAGTG GAGAGGAGGA AGGGCCAGGG
    GCAGAATCGG CCACCTTACC TGACCAGGAG GAGGTGGGGG AGGTCTGGCA GCGGAGGGGG
    AGCCTGGAGG GAGCTGCTGC TGCTGCTGCT GCTGCTGAGA GCCAGCCCCA AGAAGAGGGT
    GATGCTGGGG AAGCCCAAGG GGCTTGTGAA GGGGCCACTG CCCAGGAGGA GGGTGCTACT
    GGAGGTTCTC AAGCCAACGG CCACCAGCCA CTGCGATCTC TGCCTTCAGT GCGCCAAGAT
    GTTAGCCGGT ACCAGAGGGT GGACGAGGCT CTCCCACCAA ACTGGGAGGC CCGCATTGAC
    AGCCACGGCA GGATCTTCTA CGTGGATCAC GTGAACAGAA CCACGACGTG GCAGCGGCCC
    ACCGCTCCCC CCGCCCCGCA GGTGCTGCAG AGGTCCAACT CCATACAGCA GATGGAGCAG
    CTGAACCGGC GATACCAGAG TATCCGCAGA ACCATGACTA ACGAGAGGCC CGAGGAAAAT
    AGCAGTGCTC TTGATGGGGC AGGAGAGGAG GCCGACTTTC ACCAAGCCAG TGCAGATTTC
    CGACGGGAGA GCGTCCTGCC CCACTCTACC TCCAGATCCA GGCTCACGTT GCTGTTACAG
    TCGCCCCCTG TGAAGTTCCT CATCAGCCCA GAGTTCTTCA CCGTGCTGCA TTCGAACCCT
    AGTGCCTACC GCATGTTTAC AAACAACACG TGTTTGAAGC ACATGATCAC CAAAGTCCGG
    CGGGACACCC ACCACTTTGA ACGCTACCAG CATAACCGGG ACCTTGTGGG ATTCCTCAAC
    ATGTTTGCAA ACAAACAACT GGAGCTGCCG AGGGGCTGGG AAATGAAACA CGATCATCAG
    GGCAAGGCAT TTTTTGTTGA CCACAACTCC CGGACCACCA CTTTCATTGA CCCCCGGCTC
    CCACTCCAGA GCAGCAGACC CACGAGTGCG CTGGTGCATC GACAGCATCT GACGAGGCAG
    CGCAGCCACA GTGCAGGCGA GGTGGGAGAA GATTCTCGAC ATGCCGGACC ACCAGTTCTT
    CCCAGGCCGT CGAGTACGTT CAACACAGTC AGCAGGCCGC AGTACCAGGA CATGGTTCCA
    GTGGCTTACA ATGACAAGAT TGTTGCATTT CTTCGCCAAC CCAACATCTT TGAAATTCTA
    CAGGAGCGTC AACCTGATCT TACCAGGAAT CACTCACTCA GGGAGAAGAT CCAATTTATC
    CGAACTGAAG GGACCCCAGG ATTGGTGCGT CTTTCAAGTG ATGCAGACCT TGTTATGTTG
    CTAAGTTTAT TTGAAGAAGA GATAATGTCA TACGTGCCTC CACATGCCTT ACTCCATCCC
    AGCTACTGTC AGTCCCCACG TGGCTCCCCT GTGTCCTCTC CCCAGAACTC GCCAGGTACT
    CAGCGTGCCA ACGCCCGGGC TCCCGCCCCT TACAAGCGAG ACTTTGAAGC CAAACTGAGG
    AATTTTTACA GGAAGTTAGA GACTAAAGGA TATGGACAAG GCCCAGGGAA ATTAAAGTTA
    ATTATCAGAA GAGATCACTT GCTCGAAGAT GCTTTTAATC AGATTATGGG CTATTCCAGA
    AAAGACCTGC AGAGAAATAA GCTGTACGTC ACATTTGTCG GGGAGGAAGG GCTGGATTAC
    AGTGGACCTT CCAGAGAGTT TTTCTTCCTG GTATCCAGAG AGCTCTTTAA CCCATATTAC
    GGCTTATTTG AATATTCAGC CAATGACACA TACACAGTAC AAATAAGTCC CATGTCTGCT
    TTTGTAGACA ATCACCATGA ATGGTTCCGG TTCAGTGGTA GGATCCTTGG TCTTGCGCTG
    ATTCACCAGT ATTTGTTGGA TGCCTTCTTC ACGCGGCCCT TCTATAAGGC TCTTCTCAGA
    ATTCTGTGTG ACCTGAGTGA TCTAGAATAC CTTGATGAGG AGTTCCACCA GAGCCTGCAG
    TGGATGAAAG ACAATGATAT CCATGACATT CTGGACCTCA CGTTCACAGT GAACGAAGAA
    GTTTTCGGGC AGATAACTGA ACGAGAATTA AAACCAGGGG GTGCCAACAT CCCAGTCACA
    GAGAAGAACA AGAAGGAGTA CATCGAGAGG ATGGTGAAAT GGAGGATTGA GCGGGGTGTT
    GTGCAGCAAA CGGAGAGCTT AGTGCGTGGC TTCTACGAGG TGGTGGATGC CAGGCTAGTA
    TCTGTTTTTG ATGCAAGAGA ACTGGAACTG GTCATCGCAG GCACAGCTGA AATAGACCTA
    AGTGATTGGA GAAACAACAC AGAATACAGA GGAGGTTATC ATGACAATCA TATTGTAATT
    CGGTGGTTCT GGGCTGCAGT GGAGAGATTC AACAATGAAC AACGATTAAG GTTGTTACAG
    TTTGTCACAG GCACATCCAG CATTCCCTAT GAAGGATTTG CTTCTCTCCG AGGGAGTAAT
    GGCCCAAGAA GATTCTGCGT GGAGAAATGG GGGAAAATCA CCGCTCTTCC CAGAGCTCAC
    ACTTGTTTTA ACCGTCTGGA TCTCCCTCCA TACCCATCCT TCTCCATGCT TTATGAAAAA
    CTTTTGACAG CAGTTGAGGA GACCAGTACC TTTGGACTTG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020930204.1
    CDS116..4840
    Translation

    Target ORF information:

    RefSeq Version XM_021074545.1
    Organism Sus scrofa(pig)
    Definition Sus scrofa HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021074545.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCTAGCT CAGCCCGGGA GCACCTGCTT TTTGTGCGGC GGCGAAATCC CCAGATGCGG 
    TACACTCTGA GTCCAGAGAA CCTCCAGAGC CTGGCTGCCC AGAGCTCCAT GCCTGAGAAC
    ATGACCCTGC AGCGGGCCAA CAGCGACACA GATCTGGTGA CTTCTGAGAG CCGGTCCAGC
    CTGACTGCCA GCATGTATGA GTACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG
    GACATTAAAG AGGAGGTGGA CCCGAGTGAT TGGATTGGAC TTTATCACAT AGATGAGAAT
    TCTCCAGCCA ACTTCTGGGA TTCTAAAAAC AGGGGTGTGA CTGGAACACA AAAAGGACAA
    ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAGC CGGAGATAAA AATCTGTTTC
    AAATACTACC ACGGCATTAG TGGAGCCCTG CGAGCCACGA CCCCCTGCAT CACGGTGAAG
    AACCCTGCTG TGATGATGGG GGCAGAAGGC ATGGAAGGAG GTGCTGCAGG CAGTCAGCAT
    TCTCGGAAAC TCGTTAGCTT TACGTTGTCA GATCTTAGGG CAGTTGGGCT AAAGAAAGGG
    ATGTTCTTCA ATCCTGACCC TTATCTGAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT
    TTCCCCACCT GTGCCCACCA TGGGCAGGAG AGGAGGTCTA CCATCATCAG TAACACCACT
    AATCCAATCT GGCACCGAGA GAAATACTCC TTTTTTGCAC TCCTTACTGA TGTCTTAGAA
    ATTGAAATTA AAGACAAATT TGCCAAGAGC CGTCCTATCA TCAAGCGTTT TCTGGGGAAA
    CTAACCATCC CAGTCCAGAG GCTGTTGGAG CGACAAGCCA TCGGTGATCA GATGCTCAGC
    TACAACCTTG GCAGAAGGCT CCCAGCTGAC CACGTGAGTG GGTACCTCCA GTTCAGAGTG
    GAGGTCACGT CTTCTGTGCA TGAAGATGCT TCTCCAGAAG CAGTTGGCAC AATACTTGGA
    GTCAATACTG TGAATGGAGA CCTAGGAAGC CCTTCTGATG AGGAGGACAT GCCAGGGGGC
    CACCACGACA GCCCGGTTTG TTCTAATGGA CCAGTTTCTG AGGAAAGTGC TGATGGGACC
    CCCAAGCATT CTTTCAGGAC TAGCTCTACT CTGGAAATAG ACACGGAAGA TTTAACCTCT
    ACTTCTTCAA GGACCTCACC TCCCAGAGGA CGTCAGGATT CCCTCAACGA TTATTTAGAT
    GCTATTGAAC ACAATGGCCA TTCCAGGTCA GGTGCAGCTA CCTGCTCAGA GAGGTCCATG
    GGAGCCTCTC CAAAACTAAG GAGTAGTTTT CCCACCGACA CGAGACTCAA TGCCATGCTT
    CATATTGATT CAGATGAGGA AGACCACGAG TTCCAGCAGG ACCTGGGTTA TCCATCTTCC
    TTGGAGGAGG AAGGAGGGTT GATAATGTTT AGCAGGGCAT CAAGAACTGA TGACGGGAGC
    CTCACATCTC AGACAAAGCC GGAAGACATC CCAGTGGAGA CTGAGGAAGC CTCTATCCAC
    GAACCTGCTT CCTTTGAGGA TAAGCCAGAA CATCTTCCGG AGCTTACAGA GAGCTCCTTA
    CCCTCAGGCC CCATGCCAGA TGAAAGTGAA AATGGCCTGG AGTCTCAACC AGGTGCCGAC
    CAGGGCAGCA CTGAATTGTG TGGCTCTCAG GAGGTCGATC AGCCTACGAG TGGGGCAGAC
    ACGGGCACTT CTGACACGTC GGGAGGAAGC AGAAGAGCCA TCAGTGAGAC AGAGTCCCTT
    GATCAGGGGT CCGAGCCTTC CCAGGTGTCC TCTGAAACAG AACCCAGTGA CCCTGCCAGG
    ACAGAGAGCG TGAGTGAGGC CAGCACCAGG CCTGAGGGAG AGAGTGACCT GGAAGGGGCC
    GACAGCTCCT GCAATGAGAG TGTCACCACG CAGCTGTCCT CGGTGGAGAC ACGGTGCTCA
    TCTCTTGAGA GTGCACGGTT TCCCGAAACC CCAGCCTTTT CTTCTCAGGA GGAGGAAGAT
    GGAGCCTGTG CAGCAGAGCC CACCAGCAGT GGCCCTGCCG AGGGGTCACA GGATTCCATA
    TGCACTCCTG GGTCCTTACC TGTGGTGCAG GTGCCCAGTG GAGAGGAGGA AGGGCCAGGG
    GCAGAATCGG CCACCTTACC TGACCAGGAG GAGGTGGGGG AGGTCTGGCA GCGGAGGGGG
    AGCCTGGAGG GAGCTGCTGC TGCTGCTGCT GCTGCTGAGA GCCAGCCCCA AGAAGAGGGT
    GATGCTGGGG AAGCCCAAGG GGCTTGTGAA GGGGCCACTG CCCAGGAGGA GGGTGCTACT
    GGAGGTTCTC AAGCCAACGG CCACCAGCCA CTGCGATCTC TGCCTTCAGT GCGCCAAGAT
    GTTAGCCGGT ACCAGAGGGT GGACGAGGCT CTCCCACCAA ACTGGGAGGC CCGCATTGAC
    AGCCACGGCA GGATCTTCTA CGTGGATCAC GTGAACAGAA CCACGACGTG GCAGCGGCCC
    ACCGCTCCCC CCGCCCCGCA GGTGCTGCAG AGGTCCAACT CCATACAGCA GATGGAGCAG
    CTGAACCGGC GATACCAGAG TATCCGCAGA ACCATGACTA ACGAGAGGCC CGAGGAAAAT
    AGCAGTGCTC TTGATGGGGC AGGAGAGGAG GCCGACTTTC ACCAAGCCAG TGCAGATTTC
    CGACGGGAGA GCGTCCTGCC CCACTCTACC TCCAGATCCA GGCTCACGTT GCTGTTACAG
    TCGCCCCCTG TGAAGTTCCT CATCAGCCCA GAGTTCTTCA CCGTGCTGCA TTCGAACCCT
    AGTGCCTACC GCATGTTTAC AAACAACACG TGTTTGAAGC ACATGATCAC CAAAGTCCGG
    CGGGACACCC ACCACTTTGA ACGCTACCAG CATAACCGGG ACCTTGTGGG ATTCCTCAAC
    ATGTTTGCAA ACAAACAACT GGAGCTGCCG AGGGGCTGGG AAATGAAACA CGATCATCAG
    GGCAAGGCAT TTTTTGTTGA CCACAACTCC CGGACCACCA CTTTCATTGA CCCCCGGCTC
    CCACTCCAGA GCAGCAGACC CACGAGTGCG CTGGTGCATC GACAGCATCT GACGAGGCAG
    CGCAGCCACA GTGCAGGCGA GGTGGGAGAA GATTCTCGAC ATGCCGGACC ACCAGTTCTT
    CCCAGGCCGT CGAGTACGTT CAACACAGTC AGCAGGCCGC AGTACCAGGA CATGGTTCCA
    GTGGCTTACA ATGACAAGAT TGTTGCATTT CTTCGCCAAC CCAACATCTT TGAAATTCTA
    CAGGAGCGTC AACCTGATCT TACCAGGAAT CACTCACTCA GGGAGAAGAT CCAATTTATC
    CGAACTGAAG GGACCCCAGG ATTGGTGCGT CTTTCAAGTG ATGCAGACCT TGTTATGTTG
    CTAAGTTTAT TTGAAGAAGA GATAATGTCA TACGTGCCTC CACATGCCTT ACTCCATCCC
    AGCTACTGTC AGTCCCCACG TGGCTCCCCT GTGTCCTCTC CCCAGAACTC GCCAGGTACT
    CAGCGTGCCA ACGCCCGGGC TCCCGCCCCT TACAAGCGAG ACTTTGAAGC CAAACTGAGG
    AATTTTTACA GGAAGTTAGA GACTAAAGGA TATGGACAAG GCCCAGGGAA ATTAAAGTTA
    ATTATCAGAA GAGATCACTT GCTCGAAGAT GCTTTTAATC AGATTATGGG CTATTCCAGA
    AAAGACCTGC AGAGAAATAA GCTGTACGTC ACATTTGTCG GGGAGGAAGG GCTGGATTAC
    AGTGGACCTT CCAGAGAGTT TTTCTTCCTG GTATCCAGAG AGCTCTTTAA CCCATATTAC
    GGCTTATTTG AATATTCAGC CAATGACACA TACACAGTAC AAATAAGTCC CATGTCTGCT
    TTTGTAGACA ATCACCATGA ATGGTTCCGG TTCAGTGGTA GGATCCTTGG TCTTGCGCTG
    ATTCACCAGT ATTTGTTGGA TGCCTTCTTC ACGCGGCCCT TCTATAAGGC TCTTCTCAGA
    ATTCTGTGTG ACCTGAGTGA TCTAGAATAC CTTGATGAGG AGTTCCACCA GAGCCTGCAG
    TGGATGAAAG ACAATGATAT CCATGACATT CTGGACCTCA CGTTCACAGT GAACGAAGAA
    GTTTTCGGGC AGATAACTGA ACGAGAATTA AAACCAGGGG GTGCCAACAT CCCAGTCACA
    GAGAAGAACA AGAAGGAGTA CATCGAGAGG ATGGTGAAAT GGAGGATTGA GCGGGGTGTT
    GTGCAGCAAA CGGAGAGCTT AGTGCGTGGC TTCTACGAGG TGGTGGATGC CAGGCTAGTA
    TCTGTTTTTG ATGCAAGAGA ACTGGAACTG GTCATCGCAG GCACAGCTGA AATAGACCTA
    AGTGATTGGA GAAACAACAC AGAATACAGA GGAGGTTATC ATGACAATCA TATTGTAATT
    CGGTGGTTCT GGGCTGCAGT GGAGAGATTC AACAATGAAC AACGATTAAG GTTGTTACAG
    TTTGTCACAG GCACATCCAG CATTCCCTAT GAAGGATTTG CTTCTCTCCG AGGGAGTAAT
    GGCCCAAGAA GATTCTGCGT GGAGAAATGG GGGAAAATCA CCGCTCTTCC CAGAGCTCAC
    ACTTGTTTTA ACCGTCTGGA TCTCCCTCCA TACCCATCCT TCTCCATGCT TTATGAAAAA
    CTTTTGACAG CAGTTGAGGA GACCAGTACC TTTGGACTTG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe01255
    Clone ID Related Accession (Same CDS sequence) XM_021074541.1 , XM_021074543.1 , NM_001198924.1 , XM_021074542.1 , XM_021074545.1 , XM_021074544.1
    Accession Version XM_021074544.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4725bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product E3 ubiquitin-protein ligase HECW2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010457.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCTAGCT CAGCCCGGGA GCACCTGCTT TTTGTGCGGC GGCGAAATCC CCAGATGCGG 
    TACACTCTGA GTCCAGAGAA CCTCCAGAGC CTGGCTGCCC AGAGCTCCAT GCCTGAGAAC
    ATGACCCTGC AGCGGGCCAA CAGCGACACA GATCTGGTGA CTTCTGAGAG CCGGTCCAGC
    CTGACTGCCA GCATGTATGA GTACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG
    GACATTAAAG AGGAGGTGGA CCCGAGTGAT TGGATTGGAC TTTATCACAT AGATGAGAAT
    TCTCCAGCCA ACTTCTGGGA TTCTAAAAAC AGGGGTGTGA CTGGAACACA AAAAGGACAA
    ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAGC CGGAGATAAA AATCTGTTTC
    AAATACTACC ACGGCATTAG TGGAGCCCTG CGAGCCACGA CCCCCTGCAT CACGGTGAAG
    AACCCTGCTG TGATGATGGG GGCAGAAGGC ATGGAAGGAG GTGCTGCAGG CAGTCAGCAT
    TCTCGGAAAC TCGTTAGCTT TACGTTGTCA GATCTTAGGG CAGTTGGGCT AAAGAAAGGG
    ATGTTCTTCA ATCCTGACCC TTATCTGAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT
    TTCCCCACCT GTGCCCACCA TGGGCAGGAG AGGAGGTCTA CCATCATCAG TAACACCACT
    AATCCAATCT GGCACCGAGA GAAATACTCC TTTTTTGCAC TCCTTACTGA TGTCTTAGAA
    ATTGAAATTA AAGACAAATT TGCCAAGAGC CGTCCTATCA TCAAGCGTTT TCTGGGGAAA
    CTAACCATCC CAGTCCAGAG GCTGTTGGAG CGACAAGCCA TCGGTGATCA GATGCTCAGC
    TACAACCTTG GCAGAAGGCT CCCAGCTGAC CACGTGAGTG GGTACCTCCA GTTCAGAGTG
    GAGGTCACGT CTTCTGTGCA TGAAGATGCT TCTCCAGAAG CAGTTGGCAC AATACTTGGA
    GTCAATACTG TGAATGGAGA CCTAGGAAGC CCTTCTGATG AGGAGGACAT GCCAGGGGGC
    CACCACGACA GCCCGGTTTG TTCTAATGGA CCAGTTTCTG AGGAAAGTGC TGATGGGACC
    CCCAAGCATT CTTTCAGGAC TAGCTCTACT CTGGAAATAG ACACGGAAGA TTTAACCTCT
    ACTTCTTCAA GGACCTCACC TCCCAGAGGA CGTCAGGATT CCCTCAACGA TTATTTAGAT
    GCTATTGAAC ACAATGGCCA TTCCAGGTCA GGTGCAGCTA CCTGCTCAGA GAGGTCCATG
    GGAGCCTCTC CAAAACTAAG GAGTAGTTTT CCCACCGACA CGAGACTCAA TGCCATGCTT
    CATATTGATT CAGATGAGGA AGACCACGAG TTCCAGCAGG ACCTGGGTTA TCCATCTTCC
    TTGGAGGAGG AAGGAGGGTT GATAATGTTT AGCAGGGCAT CAAGAACTGA TGACGGGAGC
    CTCACATCTC AGACAAAGCC GGAAGACATC CCAGTGGAGA CTGAGGAAGC CTCTATCCAC
    GAACCTGCTT CCTTTGAGGA TAAGCCAGAA CATCTTCCGG AGCTTACAGA GAGCTCCTTA
    CCCTCAGGCC CCATGCCAGA TGAAAGTGAA AATGGCCTGG AGTCTCAACC AGGTGCCGAC
    CAGGGCAGCA CTGAATTGTG TGGCTCTCAG GAGGTCGATC AGCCTACGAG TGGGGCAGAC
    ACGGGCACTT CTGACACGTC GGGAGGAAGC AGAAGAGCCA TCAGTGAGAC AGAGTCCCTT
    GATCAGGGGT CCGAGCCTTC CCAGGTGTCC TCTGAAACAG AACCCAGTGA CCCTGCCAGG
    ACAGAGAGCG TGAGTGAGGC CAGCACCAGG CCTGAGGGAG AGAGTGACCT GGAAGGGGCC
    GACAGCTCCT GCAATGAGAG TGTCACCACG CAGCTGTCCT CGGTGGAGAC ACGGTGCTCA
    TCTCTTGAGA GTGCACGGTT TCCCGAAACC CCAGCCTTTT CTTCTCAGGA GGAGGAAGAT
    GGAGCCTGTG CAGCAGAGCC CACCAGCAGT GGCCCTGCCG AGGGGTCACA GGATTCCATA
    TGCACTCCTG GGTCCTTACC TGTGGTGCAG GTGCCCAGTG GAGAGGAGGA AGGGCCAGGG
    GCAGAATCGG CCACCTTACC TGACCAGGAG GAGGTGGGGG AGGTCTGGCA GCGGAGGGGG
    AGCCTGGAGG GAGCTGCTGC TGCTGCTGCT GCTGCTGAGA GCCAGCCCCA AGAAGAGGGT
    GATGCTGGGG AAGCCCAAGG GGCTTGTGAA GGGGCCACTG CCCAGGAGGA GGGTGCTACT
    GGAGGTTCTC AAGCCAACGG CCACCAGCCA CTGCGATCTC TGCCTTCAGT GCGCCAAGAT
    GTTAGCCGGT ACCAGAGGGT GGACGAGGCT CTCCCACCAA ACTGGGAGGC CCGCATTGAC
    AGCCACGGCA GGATCTTCTA CGTGGATCAC GTGAACAGAA CCACGACGTG GCAGCGGCCC
    ACCGCTCCCC CCGCCCCGCA GGTGCTGCAG AGGTCCAACT CCATACAGCA GATGGAGCAG
    CTGAACCGGC GATACCAGAG TATCCGCAGA ACCATGACTA ACGAGAGGCC CGAGGAAAAT
    AGCAGTGCTC TTGATGGGGC AGGAGAGGAG GCCGACTTTC ACCAAGCCAG TGCAGATTTC
    CGACGGGAGA GCGTCCTGCC CCACTCTACC TCCAGATCCA GGCTCACGTT GCTGTTACAG
    TCGCCCCCTG TGAAGTTCCT CATCAGCCCA GAGTTCTTCA CCGTGCTGCA TTCGAACCCT
    AGTGCCTACC GCATGTTTAC AAACAACACG TGTTTGAAGC ACATGATCAC CAAAGTCCGG
    CGGGACACCC ACCACTTTGA ACGCTACCAG CATAACCGGG ACCTTGTGGG ATTCCTCAAC
    ATGTTTGCAA ACAAACAACT GGAGCTGCCG AGGGGCTGGG AAATGAAACA CGATCATCAG
    GGCAAGGCAT TTTTTGTTGA CCACAACTCC CGGACCACCA CTTTCATTGA CCCCCGGCTC
    CCACTCCAGA GCAGCAGACC CACGAGTGCG CTGGTGCATC GACAGCATCT GACGAGGCAG
    CGCAGCCACA GTGCAGGCGA GGTGGGAGAA GATTCTCGAC ATGCCGGACC ACCAGTTCTT
    CCCAGGCCGT CGAGTACGTT CAACACAGTC AGCAGGCCGC AGTACCAGGA CATGGTTCCA
    GTGGCTTACA ATGACAAGAT TGTTGCATTT CTTCGCCAAC CCAACATCTT TGAAATTCTA
    CAGGAGCGTC AACCTGATCT TACCAGGAAT CACTCACTCA GGGAGAAGAT CCAATTTATC
    CGAACTGAAG GGACCCCAGG ATTGGTGCGT CTTTCAAGTG ATGCAGACCT TGTTATGTTG
    CTAAGTTTAT TTGAAGAAGA GATAATGTCA TACGTGCCTC CACATGCCTT ACTCCATCCC
    AGCTACTGTC AGTCCCCACG TGGCTCCCCT GTGTCCTCTC CCCAGAACTC GCCAGGTACT
    CAGCGTGCCA ACGCCCGGGC TCCCGCCCCT TACAAGCGAG ACTTTGAAGC CAAACTGAGG
    AATTTTTACA GGAAGTTAGA GACTAAAGGA TATGGACAAG GCCCAGGGAA ATTAAAGTTA
    ATTATCAGAA GAGATCACTT GCTCGAAGAT GCTTTTAATC AGATTATGGG CTATTCCAGA
    AAAGACCTGC AGAGAAATAA GCTGTACGTC ACATTTGTCG GGGAGGAAGG GCTGGATTAC
    AGTGGACCTT CCAGAGAGTT TTTCTTCCTG GTATCCAGAG AGCTCTTTAA CCCATATTAC
    GGCTTATTTG AATATTCAGC CAATGACACA TACACAGTAC AAATAAGTCC CATGTCTGCT
    TTTGTAGACA ATCACCATGA ATGGTTCCGG TTCAGTGGTA GGATCCTTGG TCTTGCGCTG
    ATTCACCAGT ATTTGTTGGA TGCCTTCTTC ACGCGGCCCT TCTATAAGGC TCTTCTCAGA
    ATTCTGTGTG ACCTGAGTGA TCTAGAATAC CTTGATGAGG AGTTCCACCA GAGCCTGCAG
    TGGATGAAAG ACAATGATAT CCATGACATT CTGGACCTCA CGTTCACAGT GAACGAAGAA
    GTTTTCGGGC AGATAACTGA ACGAGAATTA AAACCAGGGG GTGCCAACAT CCCAGTCACA
    GAGAAGAACA AGAAGGAGTA CATCGAGAGG ATGGTGAAAT GGAGGATTGA GCGGGGTGTT
    GTGCAGCAAA CGGAGAGCTT AGTGCGTGGC TTCTACGAGG TGGTGGATGC CAGGCTAGTA
    TCTGTTTTTG ATGCAAGAGA ACTGGAACTG GTCATCGCAG GCACAGCTGA AATAGACCTA
    AGTGATTGGA GAAACAACAC AGAATACAGA GGAGGTTATC ATGACAATCA TATTGTAATT
    CGGTGGTTCT GGGCTGCAGT GGAGAGATTC AACAATGAAC AACGATTAAG GTTGTTACAG
    TTTGTCACAG GCACATCCAG CATTCCCTAT GAAGGATTTG CTTCTCTCCG AGGGAGTAAT
    GGCCCAAGAA GATTCTGCGT GGAGAAATGG GGGAAAATCA CCGCTCTTCC CAGAGCTCAC
    ACTTGTTTTA ACCGTCTGGA TCTCCCTCCA TACCCATCCT TCTCCATGCT TTATGAAAAA
    CTTTTGACAG CAGTTGAGGA GACCAGTACC TTTGGACTTG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020930203.1
    CDS358..5082
    Translation

    Target ORF information:

    RefSeq Version XM_021074544.1
    Organism Sus scrofa(pig)
    Definition Sus scrofa HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021074544.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCTAGCT CAGCCCGGGA GCACCTGCTT TTTGTGCGGC GGCGAAATCC CCAGATGCGG 
    TACACTCTGA GTCCAGAGAA CCTCCAGAGC CTGGCTGCCC AGAGCTCCAT GCCTGAGAAC
    ATGACCCTGC AGCGGGCCAA CAGCGACACA GATCTGGTGA CTTCTGAGAG CCGGTCCAGC
    CTGACTGCCA GCATGTATGA GTACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG
    GACATTAAAG AGGAGGTGGA CCCGAGTGAT TGGATTGGAC TTTATCACAT AGATGAGAAT
    TCTCCAGCCA ACTTCTGGGA TTCTAAAAAC AGGGGTGTGA CTGGAACACA AAAAGGACAA
    ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAGC CGGAGATAAA AATCTGTTTC
    AAATACTACC ACGGCATTAG TGGAGCCCTG CGAGCCACGA CCCCCTGCAT CACGGTGAAG
    AACCCTGCTG TGATGATGGG GGCAGAAGGC ATGGAAGGAG GTGCTGCAGG CAGTCAGCAT
    TCTCGGAAAC TCGTTAGCTT TACGTTGTCA GATCTTAGGG CAGTTGGGCT AAAGAAAGGG
    ATGTTCTTCA ATCCTGACCC TTATCTGAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT
    TTCCCCACCT GTGCCCACCA TGGGCAGGAG AGGAGGTCTA CCATCATCAG TAACACCACT
    AATCCAATCT GGCACCGAGA GAAATACTCC TTTTTTGCAC TCCTTACTGA TGTCTTAGAA
    ATTGAAATTA AAGACAAATT TGCCAAGAGC CGTCCTATCA TCAAGCGTTT TCTGGGGAAA
    CTAACCATCC CAGTCCAGAG GCTGTTGGAG CGACAAGCCA TCGGTGATCA GATGCTCAGC
    TACAACCTTG GCAGAAGGCT CCCAGCTGAC CACGTGAGTG GGTACCTCCA GTTCAGAGTG
    GAGGTCACGT CTTCTGTGCA TGAAGATGCT TCTCCAGAAG CAGTTGGCAC AATACTTGGA
    GTCAATACTG TGAATGGAGA CCTAGGAAGC CCTTCTGATG AGGAGGACAT GCCAGGGGGC
    CACCACGACA GCCCGGTTTG TTCTAATGGA CCAGTTTCTG AGGAAAGTGC TGATGGGACC
    CCCAAGCATT CTTTCAGGAC TAGCTCTACT CTGGAAATAG ACACGGAAGA TTTAACCTCT
    ACTTCTTCAA GGACCTCACC TCCCAGAGGA CGTCAGGATT CCCTCAACGA TTATTTAGAT
    GCTATTGAAC ACAATGGCCA TTCCAGGTCA GGTGCAGCTA CCTGCTCAGA GAGGTCCATG
    GGAGCCTCTC CAAAACTAAG GAGTAGTTTT CCCACCGACA CGAGACTCAA TGCCATGCTT
    CATATTGATT CAGATGAGGA AGACCACGAG TTCCAGCAGG ACCTGGGTTA TCCATCTTCC
    TTGGAGGAGG AAGGAGGGTT GATAATGTTT AGCAGGGCAT CAAGAACTGA TGACGGGAGC
    CTCACATCTC AGACAAAGCC GGAAGACATC CCAGTGGAGA CTGAGGAAGC CTCTATCCAC
    GAACCTGCTT CCTTTGAGGA TAAGCCAGAA CATCTTCCGG AGCTTACAGA GAGCTCCTTA
    CCCTCAGGCC CCATGCCAGA TGAAAGTGAA AATGGCCTGG AGTCTCAACC AGGTGCCGAC
    CAGGGCAGCA CTGAATTGTG TGGCTCTCAG GAGGTCGATC AGCCTACGAG TGGGGCAGAC
    ACGGGCACTT CTGACACGTC GGGAGGAAGC AGAAGAGCCA TCAGTGAGAC AGAGTCCCTT
    GATCAGGGGT CCGAGCCTTC CCAGGTGTCC TCTGAAACAG AACCCAGTGA CCCTGCCAGG
    ACAGAGAGCG TGAGTGAGGC CAGCACCAGG CCTGAGGGAG AGAGTGACCT GGAAGGGGCC
    GACAGCTCCT GCAATGAGAG TGTCACCACG CAGCTGTCCT CGGTGGAGAC ACGGTGCTCA
    TCTCTTGAGA GTGCACGGTT TCCCGAAACC CCAGCCTTTT CTTCTCAGGA GGAGGAAGAT
    GGAGCCTGTG CAGCAGAGCC CACCAGCAGT GGCCCTGCCG AGGGGTCACA GGATTCCATA
    TGCACTCCTG GGTCCTTACC TGTGGTGCAG GTGCCCAGTG GAGAGGAGGA AGGGCCAGGG
    GCAGAATCGG CCACCTTACC TGACCAGGAG GAGGTGGGGG AGGTCTGGCA GCGGAGGGGG
    AGCCTGGAGG GAGCTGCTGC TGCTGCTGCT GCTGCTGAGA GCCAGCCCCA AGAAGAGGGT
    GATGCTGGGG AAGCCCAAGG GGCTTGTGAA GGGGCCACTG CCCAGGAGGA GGGTGCTACT
    GGAGGTTCTC AAGCCAACGG CCACCAGCCA CTGCGATCTC TGCCTTCAGT GCGCCAAGAT
    GTTAGCCGGT ACCAGAGGGT GGACGAGGCT CTCCCACCAA ACTGGGAGGC CCGCATTGAC
    AGCCACGGCA GGATCTTCTA CGTGGATCAC GTGAACAGAA CCACGACGTG GCAGCGGCCC
    ACCGCTCCCC CCGCCCCGCA GGTGCTGCAG AGGTCCAACT CCATACAGCA GATGGAGCAG
    CTGAACCGGC GATACCAGAG TATCCGCAGA ACCATGACTA ACGAGAGGCC CGAGGAAAAT
    AGCAGTGCTC TTGATGGGGC AGGAGAGGAG GCCGACTTTC ACCAAGCCAG TGCAGATTTC
    CGACGGGAGA GCGTCCTGCC CCACTCTACC TCCAGATCCA GGCTCACGTT GCTGTTACAG
    TCGCCCCCTG TGAAGTTCCT CATCAGCCCA GAGTTCTTCA CCGTGCTGCA TTCGAACCCT
    AGTGCCTACC GCATGTTTAC AAACAACACG TGTTTGAAGC ACATGATCAC CAAAGTCCGG
    CGGGACACCC ACCACTTTGA ACGCTACCAG CATAACCGGG ACCTTGTGGG ATTCCTCAAC
    ATGTTTGCAA ACAAACAACT GGAGCTGCCG AGGGGCTGGG AAATGAAACA CGATCATCAG
    GGCAAGGCAT TTTTTGTTGA CCACAACTCC CGGACCACCA CTTTCATTGA CCCCCGGCTC
    CCACTCCAGA GCAGCAGACC CACGAGTGCG CTGGTGCATC GACAGCATCT GACGAGGCAG
    CGCAGCCACA GTGCAGGCGA GGTGGGAGAA GATTCTCGAC ATGCCGGACC ACCAGTTCTT
    CCCAGGCCGT CGAGTACGTT CAACACAGTC AGCAGGCCGC AGTACCAGGA CATGGTTCCA
    GTGGCTTACA ATGACAAGAT TGTTGCATTT CTTCGCCAAC CCAACATCTT TGAAATTCTA
    CAGGAGCGTC AACCTGATCT TACCAGGAAT CACTCACTCA GGGAGAAGAT CCAATTTATC
    CGAACTGAAG GGACCCCAGG ATTGGTGCGT CTTTCAAGTG ATGCAGACCT TGTTATGTTG
    CTAAGTTTAT TTGAAGAAGA GATAATGTCA TACGTGCCTC CACATGCCTT ACTCCATCCC
    AGCTACTGTC AGTCCCCACG TGGCTCCCCT GTGTCCTCTC CCCAGAACTC GCCAGGTACT
    CAGCGTGCCA ACGCCCGGGC TCCCGCCCCT TACAAGCGAG ACTTTGAAGC CAAACTGAGG
    AATTTTTACA GGAAGTTAGA GACTAAAGGA TATGGACAAG GCCCAGGGAA ATTAAAGTTA
    ATTATCAGAA GAGATCACTT GCTCGAAGAT GCTTTTAATC AGATTATGGG CTATTCCAGA
    AAAGACCTGC AGAGAAATAA GCTGTACGTC ACATTTGTCG GGGAGGAAGG GCTGGATTAC
    AGTGGACCTT CCAGAGAGTT TTTCTTCCTG GTATCCAGAG AGCTCTTTAA CCCATATTAC
    GGCTTATTTG AATATTCAGC CAATGACACA TACACAGTAC AAATAAGTCC CATGTCTGCT
    TTTGTAGACA ATCACCATGA ATGGTTCCGG TTCAGTGGTA GGATCCTTGG TCTTGCGCTG
    ATTCACCAGT ATTTGTTGGA TGCCTTCTTC ACGCGGCCCT TCTATAAGGC TCTTCTCAGA
    ATTCTGTGTG ACCTGAGTGA TCTAGAATAC CTTGATGAGG AGTTCCACCA GAGCCTGCAG
    TGGATGAAAG ACAATGATAT CCATGACATT CTGGACCTCA CGTTCACAGT GAACGAAGAA
    GTTTTCGGGC AGATAACTGA ACGAGAATTA AAACCAGGGG GTGCCAACAT CCCAGTCACA
    GAGAAGAACA AGAAGGAGTA CATCGAGAGG ATGGTGAAAT GGAGGATTGA GCGGGGTGTT
    GTGCAGCAAA CGGAGAGCTT AGTGCGTGGC TTCTACGAGG TGGTGGATGC CAGGCTAGTA
    TCTGTTTTTG ATGCAAGAGA ACTGGAACTG GTCATCGCAG GCACAGCTGA AATAGACCTA
    AGTGATTGGA GAAACAACAC AGAATACAGA GGAGGTTATC ATGACAATCA TATTGTAATT
    CGGTGGTTCT GGGCTGCAGT GGAGAGATTC AACAATGAAC AACGATTAAG GTTGTTACAG
    TTTGTCACAG GCACATCCAG CATTCCCTAT GAAGGATTTG CTTCTCTCCG AGGGAGTAAT
    GGCCCAAGAA GATTCTGCGT GGAGAAATGG GGGAAAATCA CCGCTCTTCC CAGAGCTCAC
    ACTTGTTTTA ACCGTCTGGA TCTCCCTCCA TACCCATCCT TCTCCATGCT TTATGAAAAA
    CTTTTGACAG CAGTTGAGGA GACCAGTACC TTTGGACTTG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.