HECW2 cDNA ORF clone, Sus scrofa(pig)

Gene
Clones
gRNAs

Summary

Gene Symbol	HECW2
Entrez Gene ID	100155879
Full Name	HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2
Gene Type	protein-coding
Organism	Sus scrofa(pig)
Genome	Chromosome: 15 Map Location: 15\|15

mRNA and Protein(s)

mRNA	Protein	Name
XM_021074546.1	XP_020930205.1	E3 ubiquitin-protein ligase HECW2 isoform X2
XM_021074541.1	XP_020930200.1	E3 ubiquitin-protein ligase HECW2 isoform X1
XM_021074543.1	XP_020930202.1	E3 ubiquitin-protein ligase HECW2 isoform X1
NM_001198924.1	NP_001185853.1	E3 ubiquitin-protein ligase HECW2
XM_021074542.1	XP_020930201.1	E3 ubiquitin-protein ligase HECW2 isoform X1
XM_021074545.1	XP_020930204.1	E3 ubiquitin-protein ligase HECW2 isoform X1
XM_021074544.1	XP_020930203.1	E3 ubiquitin-protein ligase HECW2 isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Knobbed acrosome defect is associated with a region containing the genes STK17b and HECW2 on porcine chromosome 15.
Sironen A, Uimari P, Nagy S, Paku S, Andersson M, Vilkki J
BMC genomics11699(2010 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Two candidate genes STK17b and HECW2 for a severe defect affecting both the sperm acrosome and chromatin that causes infertility, were identified.
Title: Knobbed acrosome defect is associated with a region containing the genes STK17b and HECW2 on porcine chromosome 15.

The following HECW2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HECW2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSe239004	XM_021074546.1 Latest version!	Sus scrofa HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OSe01255	NM_001198924.1 Latest version!	Sus scrofa HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OSe01255	XM_021074544.1 Latest version!	Sus scrofa HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OSe01255	XM_021074543.1 Latest version!	Sus scrofa HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OSe01255	XM_021074541.1 Latest version!	Sus scrofa HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OSe01255	XM_021074545.1 Latest version!	Sus scrofa HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OSe01255	XM_021074542.1 Latest version!	Sus scrofa HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HECW2 ( XM_021074546.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020930205.1 Sus scrofa HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OSe239004
Clone ID Related Accession (Same CDS sequence)	XM_021074546.1
Accession Version	XM_021074546.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4332bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Sus scrofa(pig)
Product	E3 ubiquitin-protein ligase HECW2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010457.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCGG AGATAAAAAT CTGTTTCAAA TACTACCACG GCATTAGTGG AGCCCTGCGA 
GCCACGACCC CCTGCATCAC GGTGAAGAAC CCTGCTGTGA TGATGGGGGC AGAAGGCATG 
GAAGGAGGTG CTGCAGGCAG TCAGCATTCT CGGAAACTCG TTAGCTTTAC GTTGTCAGAT 
CTTAGGGCAG TTGGGCTAAA GAAAGGGATG TTCTTCAATC CTGACCCTTA TCTGAAGATG 
TCCATTCAGC CAGGGAAGAA GAGCAGTTTC CCCACCTGTG CCCACCATGG GCAGGAGAGG 
AGGTCTACCA TCATCAGTAA CACCACTAAT CCAATCTGGC ACCGAGAGAA ATACTCCTTT 
TTTGCACTCC TTACTGATGT CTTAGAAATT GAAATTAAAG ACAAATTTGC CAAGAGCCGT 
CCTATCATCA AGCGTTTTCT GGGGAAACTA ACCATCCCAG TCCAGAGGCT GTTGGAGCGA 
CAAGCCATCG GTGATCAGAT GCTCAGCTAC AACCTTGGCA GAAGGCTCCC AGCTGACCAC 
GTGAGTGGGT ACCTCCAGTT CAGAGTGGAG GTCACGTCTT CTGTGCATGA AGATGCTTCT 
CCAGAAGCAG TTGGCACAAT ACTTGGAGTC AATACTGTGA ATGGAGACCT AGGAAGCCCT 
TCTGATGAGG AGGACATGCC AGGGGGCCAC CACGACAGCC CGGTTTGTTC TAATGGACCA 
GTTTCTGAGG AAAGTGCTGA TGGGACCCCC AAGCATTCTT TCAGGACTAG CTCTACTCTG 
GAAATAGACA CGGAAGATTT AACCTCTACT TCTTCAAGGA CCTCACCTCC CAGAGGACGT 
CAGGATTCCC TCAACGATTA TTTAGATGCT ATTGAACACA ATGGCCATTC CAGGTCAGGT 
GCAGCTACCT GCTCAGAGAG GTCCATGGGA GCCTCTCCAA AACTAAGGAG TAGTTTTCCC 
ACCGACACGA GACTCAATGC CATGCTTCAT ATTGATTCAG ATGAGGAAGA CCACGAGTTC 
CAGCAGGACC TGGGTTATCC ATCTTCCTTG GAGGAGGAAG GAGGGTTGAT AATGTTTAGC 
AGGGCATCAA GAACTGATGA CGGGAGCCTC ACATCTCAGA CAAAGCCGGA AGACATCCCA 
GTGGAGACTG AGGAAGCCTC TATCCACGAA CCTGCTTCCT TTGAGGATAA GCCAGAACAT 
CTTCCGGAGC TTACAGAGAG CTCCTTACCC TCAGGCCCCA TGCCAGATGA AAGTGAAAAT 
GGCCTGGAGT CTCAACCAGG TGCCGACCAG GGCAGCACTG AATTGTGTGG CTCTCAGGAG 
GTCGATCAGC CTACGAGTGG GGCAGACACG GGCACTTCTG ACACGTCGGG AGGAAGCAGA 
AGAGCCATCA GTGAGACAGA GTCCCTTGAT CAGGGGTCCG AGCCTTCCCA GGTGTCCTCT 
GAAACAGAAC CCAGTGACCC TGCCAGGACA GAGAGCGTGA GTGAGGCCAG CACCAGGCCT 
GAGGGAGAGA GTGACCTGGA AGGGGCCGAC AGCTCCTGCA ATGAGAGTGT CACCACGCAG 
CTGTCCTCGG TGGAGACACG GTGCTCATCT CTTGAGAGTG CACGGTTTCC CGAAACCCCA 
GCCTTTTCTT CTCAGGAGGA GGAAGATGGA GCCTGTGCAG CAGAGCCCAC CAGCAGTGGC 
CCTGCCGAGG GGTCACAGGA TTCCATATGC ACTCCTGGGT CCTTACCTGT GGTGCAGGTG 
CCCAGTGGAG AGGAGGAAGG GCCAGGGGCA GAATCGGCCA CCTTACCTGA CCAGGAGGAG 
GTGGGGGAGG TCTGGCAGCG GAGGGGGAGC CTGGAGGGAG CTGCTGCTGC TGCTGCTGCT 
GCTGAGAGCC AGCCCCAAGA AGAGGGTGAT GCTGGGGAAG CCCAAGGGGC TTGTGAAGGG 
GCCACTGCCC AGGAGGAGGG TGCTACTGGA GGTTCTCAAG CCAACGGCCA CCAGCCACTG 
CGATCTCTGC CTTCAGTGCG CCAAGATGTT AGCCGGTACC AGAGGGTGGA CGAGGCTCTC 
CCACCAAACT GGGAGGCCCG CATTGACAGC CACGGCAGGA TCTTCTACGT GGATCACGTG 
AACAGAACCA CGACGTGGCA GCGGCCCACC GCTCCCCCCG CCCCGCAGGT GCTGCAGAGG 
TCCAACTCCA TACAGCAGAT GGAGCAGCTG AACCGGCGAT ACCAGAGTAT CCGCAGAACC 
ATGACTAACG AGAGGCCCGA GGAAAATAGC AGTGCTCTTG ATGGGGCAGG AGAGGAGGCC 
GACTTTCACC AAGCCAGTGC AGATTTCCGA CGGGAGAGCG TCCTGCCCCA CTCTACCTCC 
AGATCCAGGC TCACGTTGCT GTTACAGTCG CCCCCTGTGA AGTTCCTCAT CAGCCCAGAG 
TTCTTCACCG TGCTGCATTC GAACCCTAGT GCCTACCGCA TGTTTACAAA CAACACGTGT 
TTGAAGCACA TGATCACCAA AGTCCGGCGG GACACCCACC ACTTTGAACG CTACCAGCAT 
AACCGGGACC TTGTGGGATT CCTCAACATG TTTGCAAACA AACAACTGGA GCTGCCGAGG 
GGCTGGGAAA TGAAACACGA TCATCAGGGC AAGGCATTTT TTGTTGACCA CAACTCCCGG 
ACCACCACTT TCATTGACCC CCGGCTCCCA CTCCAGAGCA GCAGACCCAC GAGTGCGCTG 
GTGCATCGAC AGCATCTGAC GAGGCAGCGC AGCCACAGTG CAGGCGAGGT GGGAGAAGAT 
TCTCGACATG CCGGACCACC AGTTCTTCCC AGGCCGTCGA GTACGTTCAA CACAGTCAGC 
AGGCCGCAGT ACCAGGACAT GGTTCCAGTG GCTTACAATG ACAAGATTGT TGCATTTCTT 
CGCCAACCCA ACATCTTTGA AATTCTACAG GAGCGTCAAC CTGATCTTAC CAGGAATCAC 
TCACTCAGGG AGAAGATCCA ATTTATCCGA ACTGAAGGGA CCCCAGGATT GGTGCGTCTT 
TCAAGTGATG CAGACCTTGT TATGTTGCTA AGTTTATTTG AAGAAGAGAT AATGTCATAC 
GTGCCTCCAC ATGCCTTACT CCATCCCAGC TACTGTCAGT CCCCACGTGG CTCCCCTGTG 
TCCTCTCCCC AGAACTCGCC AGGTACTCAG CGTGCCAACG CCCGGGCTCC CGCCCCTTAC 
AAGCGAGACT TTGAAGCCAA ACTGAGGAAT TTTTACAGGA AGTTAGAGAC TAAAGGATAT 
GGACAAGGCC CAGGGAAATT AAAGTTAATT ATCAGAAGAG ATCACTTGCT CGAAGATGCT 
TTTAATCAGA TTATGGGCTA TTCCAGAAAA GACCTGCAGA GAAATAAGCT GTACGTCACA 
TTTGTCGGGG AGGAAGGGCT GGATTACAGT GGACCTTCCA GAGAGTTTTT CTTCCTGGTA 
TCCAGAGAGC TCTTTAACCC ATATTACGGC TTATTTGAAT ATTCAGCCAA TGACACATAC 
ACAGTACAAA TAAGTCCCAT GTCTGCTTTT GTAGACAATC ACCATGAATG GTTCCGGTTC 
AGTGGTAGGA TCCTTGGTCT TGCGCTGATT CACCAGTATT TGTTGGATGC CTTCTTCACG 
CGGCCCTTCT ATAAGGCTCT TCTCAGAATT CTGTGTGACC TGAGTGATCT AGAATACCTT 
GATGAGGAGT TCCACCAGAG CCTGCAGTGG ATGAAAGACA ATGATATCCA TGACATTCTG 
GACCTCACGT TCACAGTGAA CGAAGAAGTT TTCGGGCAGA TAACTGAACG AGAATTAAAA 
CCAGGGGGTG CCAACATCCC AGTCACAGAG AAGAACAAGA AGGAGTACAT CGAGAGGATG 
GTGAAATGGA GGATTGAGCG GGGTGTTGTG CAGCAAACGG AGAGCTTAGT GCGTGGCTTC 
TACGAGGTGG TGGATGCCAG GCTAGTATCT GTTTTTGATG CAAGAGAACT GGAACTGGTC 
ATCGCAGGCA CAGCTGAAAT AGACCTAAGT GATTGGAGAA ACAACACAGA ATACAGAGGA 
GGTTATCATG ACAATCATAT TGTAATTCGG TGGTTCTGGG CTGCAGTGGA GAGATTCAAC 
AATGAACAAC GATTAAGGTT GTTACAGTTT GTCACAGGCA CATCCAGCAT TCCCTATGAA 
GGATTTGCTT CTCTCCGAGG GAGTAATGGC CCAAGAAGAT TCTGCGTGGA GAAATGGGGG 
AAAATCACCG CTCTTCCCAG AGCTCACACT TGTTTTAACC GTCTGGATCT CCCTCCATAC 
CCATCCTTCT CCATGCTTTA TGAAAAACTT TTGACAGCAG TTGAGGAGAC CAGTACCTTT 
GGACTTGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020930205.1
CDS	906..5237
Translation	MEPEIKICFKYYHGISGALRATTPCITVKNPAVMMGAEGMEGGAAGSQHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTCAHHGQERRSTIISNTTNPIWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAIGDQMLSYNLGRRLPADHVSGYLQFRVEVTSSVHEDASPEAVGTILGVNTVNGDLGSPSDEEDMPGGHHDSPVCSNGPVSEESADGTPKHSFRTSSTLEIDTEDLTSTSSRTSPPRGRQDSLNDYLDAIEHNGHSRSGAATCSERSMGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMFSRASRTDDGSLTSQTKPEDIPVETEEASIHEPASFEDKPEHLPELTESSLPSGPMPDESENGLESQPGADQGSTELCGSQEVDQPTSGADTGTSDTSGGSRRAISETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDLEGADSSCNESVTTQLSSVETRCSSLESARFPETPAFSSQEEEDGACAAEPTSSGPAEGSQDSICTPGSLPVVQVPSGEEEGPGAESATLPDQEEVGEVWQRRGSLEGAAAAAAAAESQPQEEGDAGEAQGACEGATAQEEGATGGSQANGHQPLRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENSSALDGAGEEADFHQASADFRRESVLPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNIFEILQERQPDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_021074546.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021074546.1

ORF Insert Sequence:

ATGGAGCCGG AGATAAAAAT CTGTTTCAAA TACTACCACG GCATTAGTGG AGCCCTGCGA 
GCCACGACCC CCTGCATCAC GGTGAAGAAC CCTGCTGTGA TGATGGGGGC AGAAGGCATG 
GAAGGAGGTG CTGCAGGCAG TCAGCATTCT CGGAAACTCG TTAGCTTTAC GTTGTCAGAT 
CTTAGGGCAG TTGGGCTAAA GAAAGGGATG TTCTTCAATC CTGACCCTTA TCTGAAGATG 
TCCATTCAGC CAGGGAAGAA GAGCAGTTTC CCCACCTGTG CCCACCATGG GCAGGAGAGG 
AGGTCTACCA TCATCAGTAA CACCACTAAT CCAATCTGGC ACCGAGAGAA ATACTCCTTT 
TTTGCACTCC TTACTGATGT CTTAGAAATT GAAATTAAAG ACAAATTTGC CAAGAGCCGT 
CCTATCATCA AGCGTTTTCT GGGGAAACTA ACCATCCCAG TCCAGAGGCT GTTGGAGCGA 
CAAGCCATCG GTGATCAGAT GCTCAGCTAC AACCTTGGCA GAAGGCTCCC AGCTGACCAC 
GTGAGTGGGT ACCTCCAGTT CAGAGTGGAG GTCACGTCTT CTGTGCATGA AGATGCTTCT 
CCAGAAGCAG TTGGCACAAT ACTTGGAGTC AATACTGTGA ATGGAGACCT AGGAAGCCCT 
TCTGATGAGG AGGACATGCC AGGGGGCCAC CACGACAGCC CGGTTTGTTC TAATGGACCA 
GTTTCTGAGG AAAGTGCTGA TGGGACCCCC AAGCATTCTT TCAGGACTAG CTCTACTCTG 
GAAATAGACA CGGAAGATTT AACCTCTACT TCTTCAAGGA CCTCACCTCC CAGAGGACGT 
CAGGATTCCC TCAACGATTA TTTAGATGCT ATTGAACACA ATGGCCATTC CAGGTCAGGT 
GCAGCTACCT GCTCAGAGAG GTCCATGGGA GCCTCTCCAA AACTAAGGAG TAGTTTTCCC 
ACCGACACGA GACTCAATGC CATGCTTCAT ATTGATTCAG ATGAGGAAGA CCACGAGTTC 
CAGCAGGACC TGGGTTATCC ATCTTCCTTG GAGGAGGAAG GAGGGTTGAT AATGTTTAGC 
AGGGCATCAA GAACTGATGA CGGGAGCCTC ACATCTCAGA CAAAGCCGGA AGACATCCCA 
GTGGAGACTG AGGAAGCCTC TATCCACGAA CCTGCTTCCT TTGAGGATAA GCCAGAACAT 
CTTCCGGAGC TTACAGAGAG CTCCTTACCC TCAGGCCCCA TGCCAGATGA AAGTGAAAAT 
GGCCTGGAGT CTCAACCAGG TGCCGACCAG GGCAGCACTG AATTGTGTGG CTCTCAGGAG 
GTCGATCAGC CTACGAGTGG GGCAGACACG GGCACTTCTG ACACGTCGGG AGGAAGCAGA 
AGAGCCATCA GTGAGACAGA GTCCCTTGAT CAGGGGTCCG AGCCTTCCCA GGTGTCCTCT 
GAAACAGAAC CCAGTGACCC TGCCAGGACA GAGAGCGTGA GTGAGGCCAG CACCAGGCCT 
GAGGGAGAGA GTGACCTGGA AGGGGCCGAC AGCTCCTGCA ATGAGAGTGT CACCACGCAG 
CTGTCCTCGG TGGAGACACG GTGCTCATCT CTTGAGAGTG CACGGTTTCC CGAAACCCCA 
GCCTTTTCTT CTCAGGAGGA GGAAGATGGA GCCTGTGCAG CAGAGCCCAC CAGCAGTGGC 
CCTGCCGAGG GGTCACAGGA TTCCATATGC ACTCCTGGGT CCTTACCTGT GGTGCAGGTG 
CCCAGTGGAG AGGAGGAAGG GCCAGGGGCA GAATCGGCCA CCTTACCTGA CCAGGAGGAG 
GTGGGGGAGG TCTGGCAGCG GAGGGGGAGC CTGGAGGGAG CTGCTGCTGC TGCTGCTGCT 
GCTGAGAGCC AGCCCCAAGA AGAGGGTGAT GCTGGGGAAG CCCAAGGGGC TTGTGAAGGG 
GCCACTGCCC AGGAGGAGGG TGCTACTGGA GGTTCTCAAG CCAACGGCCA CCAGCCACTG 
CGATCTCTGC CTTCAGTGCG CCAAGATGTT AGCCGGTACC AGAGGGTGGA CGAGGCTCTC 
CCACCAAACT GGGAGGCCCG CATTGACAGC CACGGCAGGA TCTTCTACGT GGATCACGTG 
AACAGAACCA CGACGTGGCA GCGGCCCACC GCTCCCCCCG CCCCGCAGGT GCTGCAGAGG 
TCCAACTCCA TACAGCAGAT GGAGCAGCTG AACCGGCGAT ACCAGAGTAT CCGCAGAACC 
ATGACTAACG AGAGGCCCGA GGAAAATAGC AGTGCTCTTG ATGGGGCAGG AGAGGAGGCC 
GACTTTCACC AAGCCAGTGC AGATTTCCGA CGGGAGAGCG TCCTGCCCCA CTCTACCTCC 
AGATCCAGGC TCACGTTGCT GTTACAGTCG CCCCCTGTGA AGTTCCTCAT CAGCCCAGAG 
TTCTTCACCG TGCTGCATTC GAACCCTAGT GCCTACCGCA TGTTTACAAA CAACACGTGT 
TTGAAGCACA TGATCACCAA AGTCCGGCGG GACACCCACC ACTTTGAACG CTACCAGCAT 
AACCGGGACC TTGTGGGATT CCTCAACATG TTTGCAAACA AACAACTGGA GCTGCCGAGG 
GGCTGGGAAA TGAAACACGA TCATCAGGGC AAGGCATTTT TTGTTGACCA CAACTCCCGG 
ACCACCACTT TCATTGACCC CCGGCTCCCA CTCCAGAGCA GCAGACCCAC GAGTGCGCTG 
GTGCATCGAC AGCATCTGAC GAGGCAGCGC AGCCACAGTG CAGGCGAGGT GGGAGAAGAT 
TCTCGACATG CCGGACCACC AGTTCTTCCC AGGCCGTCGA GTACGTTCAA CACAGTCAGC 
AGGCCGCAGT ACCAGGACAT GGTTCCAGTG GCTTACAATG ACAAGATTGT TGCATTTCTT 
CGCCAACCCA ACATCTTTGA AATTCTACAG GAGCGTCAAC CTGATCTTAC CAGGAATCAC 
TCACTCAGGG AGAAGATCCA ATTTATCCGA ACTGAAGGGA CCCCAGGATT GGTGCGTCTT 
TCAAGTGATG CAGACCTTGT TATGTTGCTA AGTTTATTTG AAGAAGAGAT AATGTCATAC 
GTGCCTCCAC ATGCCTTACT CCATCCCAGC TACTGTCAGT CCCCACGTGG CTCCCCTGTG 
TCCTCTCCCC AGAACTCGCC AGGTACTCAG CGTGCCAACG CCCGGGCTCC CGCCCCTTAC 
AAGCGAGACT TTGAAGCCAA ACTGAGGAAT TTTTACAGGA AGTTAGAGAC TAAAGGATAT 
GGACAAGGCC CAGGGAAATT AAAGTTAATT ATCAGAAGAG ATCACTTGCT CGAAGATGCT 
TTTAATCAGA TTATGGGCTA TTCCAGAAAA GACCTGCAGA GAAATAAGCT GTACGTCACA 
TTTGTCGGGG AGGAAGGGCT GGATTACAGT GGACCTTCCA GAGAGTTTTT CTTCCTGGTA 
TCCAGAGAGC TCTTTAACCC ATATTACGGC TTATTTGAAT ATTCAGCCAA TGACACATAC 
ACAGTACAAA TAAGTCCCAT GTCTGCTTTT GTAGACAATC ACCATGAATG GTTCCGGTTC 
AGTGGTAGGA TCCTTGGTCT TGCGCTGATT CACCAGTATT TGTTGGATGC CTTCTTCACG 
CGGCCCTTCT ATAAGGCTCT TCTCAGAATT CTGTGTGACC TGAGTGATCT AGAATACCTT 
GATGAGGAGT TCCACCAGAG CCTGCAGTGG ATGAAAGACA ATGATATCCA TGACATTCTG 
GACCTCACGT TCACAGTGAA CGAAGAAGTT TTCGGGCAGA TAACTGAACG AGAATTAAAA 
CCAGGGGGTG CCAACATCCC AGTCACAGAG AAGAACAAGA AGGAGTACAT CGAGAGGATG 
GTGAAATGGA GGATTGAGCG GGGTGTTGTG CAGCAAACGG AGAGCTTAGT GCGTGGCTTC 
TACGAGGTGG TGGATGCCAG GCTAGTATCT GTTTTTGATG CAAGAGAACT GGAACTGGTC 
ATCGCAGGCA CAGCTGAAAT AGACCTAAGT GATTGGAGAA ACAACACAGA ATACAGAGGA 
GGTTATCATG ACAATCATAT TGTAATTCGG TGGTTCTGGG CTGCAGTGGA GAGATTCAAC 
AATGAACAAC GATTAAGGTT GTTACAGTTT GTCACAGGCA CATCCAGCAT TCCCTATGAA 
GGATTTGCTT CTCTCCGAGG GAGTAATGGC CCAAGAAGAT TCTGCGTGGA GAAATGGGGG 
AAAATCACCG CTCTTCCCAG AGCTCACACT TGTTTTAACC GTCTGGATCT CCCTCCATAC 
CCATCCTTCT CCATGCTTTA TGAAAAACTT TTGACAGCAG TTGAGGAGAC CAGTACCTTT 
GGACTTGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW2 ( NM_001198924.1 ) cDNA ORF clone, Sus scrofa(pig) -> NP_001185853.1 Sus scrofa HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), mRNA.

Price & Availability↑

CloneID	OSe01255
Clone ID Related Accession (Same CDS sequence)	XM_021074541.1 , XM_021074543.1 , NM_001198924.1 , XM_021074542.1 , XM_021074545.1 , XM_021074544.1
Accession Version	NM_001198924.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-15
Organism	Sus scrofa(pig)
Product	E3 ubiquitin-protein ligase HECW2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from HM562353.1. ##Evidence-Data-START## Transcript exon combination :: HM562353.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCTAGCT CAGCCCGGGA GCACCTGCTT TTTGTGCGGC GGCGAAATCC CCAGATGCGG 
TACACTCTGA GTCCAGAGAA CCTCCAGAGC CTGGCTGCCC AGAGCTCCAT GCCTGAGAAC 
ATGACCCTGC AGCGGGCCAA CAGCGACACA GATCTGGTGA CTTCTGAGAG CCGGTCCAGC 
CTGACTGCCA GCATGTATGA GTACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAAG AGGAGGTGGA CCCGAGTGAT TGGATTGGAC TTTATCACAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAAAAC AGGGGTGTGA CTGGAACACA AAAAGGACAA 
ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAGC CGGAGATAAA AATCTGTTTC 
AAATACTACC ACGGCATTAG TGGAGCCCTG CGAGCCACGA CCCCCTGCAT CACGGTGAAG 
AACCCTGCTG TGATGATGGG GGCAGAAGGC ATGGAAGGAG GTGCTGCAGG CAGTCAGCAT 
TCTCGGAAAC TCGTTAGCTT TACGTTGTCA GATCTTAGGG CAGTTGGGCT AAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTGAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT 
TTCCCCACCT GTGCCCACCA TGGGCAGGAG AGGAGGTCTA CCATCATCAG TAACACCACT 
AATCCAATCT GGCACCGAGA GAAATACTCC TTTTTTGCAC TCCTTACTGA TGTCTTAGAA 
ATTGAAATTA AAGACAAATT TGCCAAGAGC CGTCCTATCA TCAAGCGTTT TCTGGGGAAA 
CTAACCATCC CAGTCCAGAG GCTGTTGGAG CGACAAGCCA TCGGTGATCA GATGCTCAGC 
TACAACCTTG GCAGAAGGCT CCCAGCTGAC CACGTGAGTG GGTACCTCCA GTTCAGAGTG 
GAGGTCACGT CTTCTGTGCA TGAAGATGCT TCTCCAGAAG CAGTTGGCAC AATACTTGGA 
GTCAATACTG TGAATGGAGA CCTAGGAAGC CCTTCTGATG AGGAGGACAT GCCAGGGGGC 
CACCACGACA GCCCGGTTTG TTCTAATGGA CCAGTTTCTG AGGAAAGTGC TGATGGGACC 
CCCAAGCATT CTTTCAGGAC TAGCTCTACT CTGGAAATAG ACACGGAAGA TTTAACCTCT 
ACTTCTTCAA GGACCTCACC TCCCAGAGGA CGTCAGGATT CCCTCAACGA TTATTTAGAT 
GCTATTGAAC ACAATGGCCA TTCCAGGTCA GGTGCAGCTA CCTGCTCAGA GAGGTCCATG 
GGAGCCTCTC CAAAACTAAG GAGTAGTTTT CCCACCGACA CGAGACTCAA TGCCATGCTT 
CATATTGATT CAGATGAGGA AGACCACGAG TTCCAGCAGG ACCTGGGTTA TCCATCTTCC 
TTGGAGGAGG AAGGAGGGTT GATAATGTTT AGCAGGGCAT CAAGAACTGA TGACGGGAGC 
CTCACATCTC AGACAAAGCC GGAAGACATC CCAGTGGAGA CTGAGGAAGC CTCTATCCAC 
GAACCTGCTT CCTTTGAGGA TAAGCCAGAA CATCTTCCGG AGCTTACAGA GAGCTCCTTA 
CCCTCAGGCC CCATGCCAGA TGAAAGTGAA AATGGCCTGG AGTCTCAACC AGGTGCCGAC 
CAGGGCAGCA CTGAATTGTG TGGCTCTCAG GAGGTCGATC AGCCTACGAG TGGGGCAGAC 
ACGGGCACTT CTGACACGTC GGGAGGAAGC AGAAGAGCCA TCAGTGAGAC AGAGTCCCTT 
GATCAGGGGT CCGAGCCTTC CCAGGTGTCC TCTGAAACAG AACCCAGTGA CCCTGCCAGG 
ACAGAGAGCG TGAGTGAGGC CAGCACCAGG CCTGAGGGAG AGAGTGACCT GGAAGGGGCC 
GACAGCTCCT GCAATGAGAG TGTCACCACG CAGCTGTCCT CGGTGGAGAC ACGGTGCTCA 
TCTCTTGAGA GTGCACGGTT TCCCGAAACC CCAGCCTTTT CTTCTCAGGA GGAGGAAGAT 
GGAGCCTGTG CAGCAGAGCC CACCAGCAGT GGCCCTGCCG AGGGGTCACA GGATTCCATA 
TGCACTCCTG GGTCCTTACC TGTGGTGCAG GTGCCCAGTG GAGAGGAGGA AGGGCCAGGG 
GCAGAATCGG CCACCTTACC TGACCAGGAG GAGGTGGGGG AGGTCTGGCA GCGGAGGGGG 
AGCCTGGAGG GAGCTGCTGC TGCTGCTGCT GCTGCTGAGA GCCAGCCCCA AGAAGAGGGT 
GATGCTGGGG AAGCCCAAGG GGCTTGTGAA GGGGCCACTG CCCAGGAGGA GGGTGCTACT 
GGAGGTTCTC AAGCCAACGG CCACCAGCCA CTGCGATCTC TGCCTTCAGT GCGCCAAGAT 
GTTAGCCGGT ACCAGAGGGT GGACGAGGCT CTCCCACCAA ACTGGGAGGC CCGCATTGAC 
AGCCACGGCA GGATCTTCTA CGTGGATCAC GTGAACAGAA CCACGACGTG GCAGCGGCCC 
ACCGCTCCCC CCGCCCCGCA GGTGCTGCAG AGGTCCAACT CCATACAGCA GATGGAGCAG 
CTGAACCGGC GATACCAGAG TATCCGCAGA ACCATGACTA ACGAGAGGCC CGAGGAAAAT 
AGCAGTGCTC TTGATGGGGC AGGAGAGGAG GCCGACTTTC ACCAAGCCAG TGCAGATTTC 
CGACGGGAGA GCGTCCTGCC CCACTCTACC TCCAGATCCA GGCTCACGTT GCTGTTACAG 
TCGCCCCCTG TGAAGTTCCT CATCAGCCCA GAGTTCTTCA CCGTGCTGCA TTCGAACCCT 
AGTGCCTACC GCATGTTTAC AAACAACACG TGTTTGAAGC ACATGATCAC CAAAGTCCGG 
CGGGACACCC ACCACTTTGA ACGCTACCAG CATAACCGGG ACCTTGTGGG ATTCCTCAAC 
ATGTTTGCAA ACAAACAACT GGAGCTGCCG AGGGGCTGGG AAATGAAACA CGATCATCAG 
GGCAAGGCAT TTTTTGTTGA CCACAACTCC CGGACCACCA CTTTCATTGA CCCCCGGCTC 
CCACTCCAGA GCAGCAGACC CACGAGTGCG CTGGTGCATC GACAGCATCT GACGAGGCAG 
CGCAGCCACA GTGCAGGCGA GGTGGGAGAA GATTCTCGAC ATGCCGGACC ACCAGTTCTT 
CCCAGGCCGT CGAGTACGTT CAACACAGTC AGCAGGCCGC AGTACCAGGA CATGGTTCCA 
GTGGCTTACA ATGACAAGAT TGTTGCATTT CTTCGCCAAC CCAACATCTT TGAAATTCTA 
CAGGAGCGTC AACCTGATCT TACCAGGAAT CACTCACTCA GGGAGAAGAT CCAATTTATC 
CGAACTGAAG GGACCCCAGG ATTGGTGCGT CTTTCAAGTG ATGCAGACCT TGTTATGTTG 
CTAAGTTTAT TTGAAGAAGA GATAATGTCA TACGTGCCTC CACATGCCTT ACTCCATCCC 
AGCTACTGTC AGTCCCCACG TGGCTCCCCT GTGTCCTCTC CCCAGAACTC GCCAGGTACT 
CAGCGTGCCA ACGCCCGGGC TCCCGCCCCT TACAAGCGAG ACTTTGAAGC CAAACTGAGG 
AATTTTTACA GGAAGTTAGA GACTAAAGGA TATGGACAAG GCCCAGGGAA ATTAAAGTTA 
ATTATCAGAA GAGATCACTT GCTCGAAGAT GCTTTTAATC AGATTATGGG CTATTCCAGA 
AAAGACCTGC AGAGAAATAA GCTGTACGTC ACATTTGTCG GGGAGGAAGG GCTGGATTAC 
AGTGGACCTT CCAGAGAGTT TTTCTTCCTG GTATCCAGAG AGCTCTTTAA CCCATATTAC 
GGCTTATTTG AATATTCAGC CAATGACACA TACACAGTAC AAATAAGTCC CATGTCTGCT 
TTTGTAGACA ATCACCATGA ATGGTTCCGG TTCAGTGGTA GGATCCTTGG TCTTGCGCTG 
ATTCACCAGT ATTTGTTGGA TGCCTTCTTC ACGCGGCCCT TCTATAAGGC TCTTCTCAGA 
ATTCTGTGTG ACCTGAGTGA TCTAGAATAC CTTGATGAGG AGTTCCACCA GAGCCTGCAG 
TGGATGAAAG ACAATGATAT CCATGACATT CTGGACCTCA CGTTCACAGT GAACGAAGAA 
GTTTTCGGGC AGATAACTGA ACGAGAATTA AAACCAGGGG GTGCCAACAT CCCAGTCACA 
GAGAAGAACA AGAAGGAGTA CATCGAGAGG ATGGTGAAAT GGAGGATTGA GCGGGGTGTT 
GTGCAGCAAA CGGAGAGCTT AGTGCGTGGC TTCTACGAGG TGGTGGATGC CAGGCTAGTA 
TCTGTTTTTG ATGCAAGAGA ACTGGAACTG GTCATCGCAG GCACAGCTGA AATAGACCTA 
AGTGATTGGA GAAACAACAC AGAATACAGA GGAGGTTATC ATGACAATCA TATTGTAATT 
CGGTGGTTCT GGGCTGCAGT GGAGAGATTC AACAATGAAC AACGATTAAG GTTGTTACAG 
TTTGTCACAG GCACATCCAG CATTCCCTAT GAAGGATTTG CTTCTCTCCG AGGGAGTAAT 
GGCCCAAGAA GATTCTGCGT GGAGAAATGG GGGAAAATCA CCGCTCTTCC CAGAGCTCAC 
ACTTGTTTTA ACCGTCTGGA TCTCCCTCCA TACCCATCCT TCTCCATGCT TTATGAAAAA 
CTTTTGACAG CAGTTGAGGA GACCAGTACC TTTGGACTTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001185853.1
CDS	3..4727
Translation	MASSAREHLLFVRRRNPQMRYTLSPENLQSLAAQSSMPENMTLQRANSDTDLVTSESRSSLTASMYEYTLGQAQNLIIFWDIKEEVDPSDWIGLYHIDENSPANFWDSKNRGVTGTQKGQIVWRIEPGPYFMEPEIKICFKYYHGISGALRATTPCITVKNPAVMMGAEGMEGGAAGSQHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTCAHHGQERRSTIISNTTNPIWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAIGDQMLSYNLGRRLPADHVSGYLQFRVEVTSSVHEDASPEAVGTILGVNTVNGDLGSPSDEEDMPGGHHDSPVCSNGPVSEESADGTPKHSFRTSSTLEIDTEDLTSTSSRTSPPRGRQDSLNDYLDAIEHNGHSRSGAATCSERSMGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMFSRASRTDDGSLTSQTKPEDIPVETEEASIHEPASFEDKPEHLPELTESSLPSGPMPDESENGLESQPGADQGSTELCGSQEVDQPTSGADTGTSDTSGGSRRAISETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDLEGADSSCNESVTTQLSSVETRCSSLESARFPETPAFSSQEEEDGACAAEPTSSGPAEGSQDSICTPGSLPVVQVPSGEEEGPGAESATLPDQEEVGEVWQRRGSLEGAAAAAAAAESQPQEEGDAGEAQGACEGATAQEEGATGGSQANGHQPLRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENSSALDGAGEEADFHQASADFRRESVLPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNIFEILQERQPDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	NM_001198924.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001198924.1

ORF Insert Sequence:

ATGGCTAGCT CAGCCCGGGA GCACCTGCTT TTTGTGCGGC GGCGAAATCC CCAGATGCGG 
TACACTCTGA GTCCAGAGAA CCTCCAGAGC CTGGCTGCCC AGAGCTCCAT GCCTGAGAAC 
ATGACCCTGC AGCGGGCCAA CAGCGACACA GATCTGGTGA CTTCTGAGAG CCGGTCCAGC 
CTGACTGCCA GCATGTATGA GTACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAAG AGGAGGTGGA CCCGAGTGAT TGGATTGGAC TTTATCACAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAAAAC AGGGGTGTGA CTGGAACACA AAAAGGACAA 
ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAGC CGGAGATAAA AATCTGTTTC 
AAATACTACC ACGGCATTAG TGGAGCCCTG CGAGCCACGA CCCCCTGCAT CACGGTGAAG 
AACCCTGCTG TGATGATGGG GGCAGAAGGC ATGGAAGGAG GTGCTGCAGG CAGTCAGCAT 
TCTCGGAAAC TCGTTAGCTT TACGTTGTCA GATCTTAGGG CAGTTGGGCT AAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTGAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT 
TTCCCCACCT GTGCCCACCA TGGGCAGGAG AGGAGGTCTA CCATCATCAG TAACACCACT 
AATCCAATCT GGCACCGAGA GAAATACTCC TTTTTTGCAC TCCTTACTGA TGTCTTAGAA 
ATTGAAATTA AAGACAAATT TGCCAAGAGC CGTCCTATCA TCAAGCGTTT TCTGGGGAAA 
CTAACCATCC CAGTCCAGAG GCTGTTGGAG CGACAAGCCA TCGGTGATCA GATGCTCAGC 
TACAACCTTG GCAGAAGGCT CCCAGCTGAC CACGTGAGTG GGTACCTCCA GTTCAGAGTG 
GAGGTCACGT CTTCTGTGCA TGAAGATGCT TCTCCAGAAG CAGTTGGCAC AATACTTGGA 
GTCAATACTG TGAATGGAGA CCTAGGAAGC CCTTCTGATG AGGAGGACAT GCCAGGGGGC 
CACCACGACA GCCCGGTTTG TTCTAATGGA CCAGTTTCTG AGGAAAGTGC TGATGGGACC 
CCCAAGCATT CTTTCAGGAC TAGCTCTACT CTGGAAATAG ACACGGAAGA TTTAACCTCT 
ACTTCTTCAA GGACCTCACC TCCCAGAGGA CGTCAGGATT CCCTCAACGA TTATTTAGAT 
GCTATTGAAC ACAATGGCCA TTCCAGGTCA GGTGCAGCTA CCTGCTCAGA GAGGTCCATG 
GGAGCCTCTC CAAAACTAAG GAGTAGTTTT CCCACCGACA CGAGACTCAA TGCCATGCTT 
CATATTGATT CAGATGAGGA AGACCACGAG TTCCAGCAGG ACCTGGGTTA TCCATCTTCC 
TTGGAGGAGG AAGGAGGGTT GATAATGTTT AGCAGGGCAT CAAGAACTGA TGACGGGAGC 
CTCACATCTC AGACAAAGCC GGAAGACATC CCAGTGGAGA CTGAGGAAGC CTCTATCCAC 
GAACCTGCTT CCTTTGAGGA TAAGCCAGAA CATCTTCCGG AGCTTACAGA GAGCTCCTTA 
CCCTCAGGCC CCATGCCAGA TGAAAGTGAA AATGGCCTGG AGTCTCAACC AGGTGCCGAC 
CAGGGCAGCA CTGAATTGTG TGGCTCTCAG GAGGTCGATC AGCCTACGAG TGGGGCAGAC 
ACGGGCACTT CTGACACGTC GGGAGGAAGC AGAAGAGCCA TCAGTGAGAC AGAGTCCCTT 
GATCAGGGGT CCGAGCCTTC CCAGGTGTCC TCTGAAACAG AACCCAGTGA CCCTGCCAGG 
ACAGAGAGCG TGAGTGAGGC CAGCACCAGG CCTGAGGGAG AGAGTGACCT GGAAGGGGCC 
GACAGCTCCT GCAATGAGAG TGTCACCACG CAGCTGTCCT CGGTGGAGAC ACGGTGCTCA 
TCTCTTGAGA GTGCACGGTT TCCCGAAACC CCAGCCTTTT CTTCTCAGGA GGAGGAAGAT 
GGAGCCTGTG CAGCAGAGCC CACCAGCAGT GGCCCTGCCG AGGGGTCACA GGATTCCATA 
TGCACTCCTG GGTCCTTACC TGTGGTGCAG GTGCCCAGTG GAGAGGAGGA AGGGCCAGGG 
GCAGAATCGG CCACCTTACC TGACCAGGAG GAGGTGGGGG AGGTCTGGCA GCGGAGGGGG 
AGCCTGGAGG GAGCTGCTGC TGCTGCTGCT GCTGCTGAGA GCCAGCCCCA AGAAGAGGGT 
GATGCTGGGG AAGCCCAAGG GGCTTGTGAA GGGGCCACTG CCCAGGAGGA GGGTGCTACT 
GGAGGTTCTC AAGCCAACGG CCACCAGCCA CTGCGATCTC TGCCTTCAGT GCGCCAAGAT 
GTTAGCCGGT ACCAGAGGGT GGACGAGGCT CTCCCACCAA ACTGGGAGGC CCGCATTGAC 
AGCCACGGCA GGATCTTCTA CGTGGATCAC GTGAACAGAA CCACGACGTG GCAGCGGCCC 
ACCGCTCCCC CCGCCCCGCA GGTGCTGCAG AGGTCCAACT CCATACAGCA GATGGAGCAG 
CTGAACCGGC GATACCAGAG TATCCGCAGA ACCATGACTA ACGAGAGGCC CGAGGAAAAT 
AGCAGTGCTC TTGATGGGGC AGGAGAGGAG GCCGACTTTC ACCAAGCCAG TGCAGATTTC 
CGACGGGAGA GCGTCCTGCC CCACTCTACC TCCAGATCCA GGCTCACGTT GCTGTTACAG 
TCGCCCCCTG TGAAGTTCCT CATCAGCCCA GAGTTCTTCA CCGTGCTGCA TTCGAACCCT 
AGTGCCTACC GCATGTTTAC AAACAACACG TGTTTGAAGC ACATGATCAC CAAAGTCCGG 
CGGGACACCC ACCACTTTGA ACGCTACCAG CATAACCGGG ACCTTGTGGG ATTCCTCAAC 
ATGTTTGCAA ACAAACAACT GGAGCTGCCG AGGGGCTGGG AAATGAAACA CGATCATCAG 
GGCAAGGCAT TTTTTGTTGA CCACAACTCC CGGACCACCA CTTTCATTGA CCCCCGGCTC 
CCACTCCAGA GCAGCAGACC CACGAGTGCG CTGGTGCATC GACAGCATCT GACGAGGCAG 
CGCAGCCACA GTGCAGGCGA GGTGGGAGAA GATTCTCGAC ATGCCGGACC ACCAGTTCTT 
CCCAGGCCGT CGAGTACGTT CAACACAGTC AGCAGGCCGC AGTACCAGGA CATGGTTCCA 
GTGGCTTACA ATGACAAGAT TGTTGCATTT CTTCGCCAAC CCAACATCTT TGAAATTCTA 
CAGGAGCGTC AACCTGATCT TACCAGGAAT CACTCACTCA GGGAGAAGAT CCAATTTATC 
CGAACTGAAG GGACCCCAGG ATTGGTGCGT CTTTCAAGTG ATGCAGACCT TGTTATGTTG 
CTAAGTTTAT TTGAAGAAGA GATAATGTCA TACGTGCCTC CACATGCCTT ACTCCATCCC 
AGCTACTGTC AGTCCCCACG TGGCTCCCCT GTGTCCTCTC CCCAGAACTC GCCAGGTACT 
CAGCGTGCCA ACGCCCGGGC TCCCGCCCCT TACAAGCGAG ACTTTGAAGC CAAACTGAGG 
AATTTTTACA GGAAGTTAGA GACTAAAGGA TATGGACAAG GCCCAGGGAA ATTAAAGTTA 
ATTATCAGAA GAGATCACTT GCTCGAAGAT GCTTTTAATC AGATTATGGG CTATTCCAGA 
AAAGACCTGC AGAGAAATAA GCTGTACGTC ACATTTGTCG GGGAGGAAGG GCTGGATTAC 
AGTGGACCTT CCAGAGAGTT TTTCTTCCTG GTATCCAGAG AGCTCTTTAA CCCATATTAC 
GGCTTATTTG AATATTCAGC CAATGACACA TACACAGTAC AAATAAGTCC CATGTCTGCT 
TTTGTAGACA ATCACCATGA ATGGTTCCGG TTCAGTGGTA GGATCCTTGG TCTTGCGCTG 
ATTCACCAGT ATTTGTTGGA TGCCTTCTTC ACGCGGCCCT TCTATAAGGC TCTTCTCAGA 
ATTCTGTGTG ACCTGAGTGA TCTAGAATAC CTTGATGAGG AGTTCCACCA GAGCCTGCAG 
TGGATGAAAG ACAATGATAT CCATGACATT CTGGACCTCA CGTTCACAGT GAACGAAGAA 
GTTTTCGGGC AGATAACTGA ACGAGAATTA AAACCAGGGG GTGCCAACAT CCCAGTCACA 
GAGAAGAACA AGAAGGAGTA CATCGAGAGG ATGGTGAAAT GGAGGATTGA GCGGGGTGTT 
GTGCAGCAAA CGGAGAGCTT AGTGCGTGGC TTCTACGAGG TGGTGGATGC CAGGCTAGTA 
TCTGTTTTTG ATGCAAGAGA ACTGGAACTG GTCATCGCAG GCACAGCTGA AATAGACCTA 
AGTGATTGGA GAAACAACAC AGAATACAGA GGAGGTTATC ATGACAATCA TATTGTAATT 
CGGTGGTTCT GGGCTGCAGT GGAGAGATTC AACAATGAAC AACGATTAAG GTTGTTACAG 
TTTGTCACAG GCACATCCAG CATTCCCTAT GAAGGATTTG CTTCTCTCCG AGGGAGTAAT 
GGCCCAAGAA GATTCTGCGT GGAGAAATGG GGGAAAATCA CCGCTCTTCC CAGAGCTCAC 
ACTTGTTTTA ACCGTCTGGA TCTCCCTCCA TACCCATCCT TCTCCATGCT TTATGAAAAA 
CTTTTGACAG CAGTTGAGGA GACCAGTACC TTTGGACTTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW2 ( XM_021074541.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020930200.1 Sus scrofa HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X1, mRNA.

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CloneID	OSe01255
Clone ID Related Accession (Same CDS sequence)	XM_021074541.1 , XM_021074543.1 , NM_001198924.1 , XM_021074542.1 , XM_021074545.1 , XM_021074544.1
Accession Version	XM_021074541.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Sus scrofa(pig)
Product	E3 ubiquitin-protein ligase HECW2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010457.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAGCT CAGCCCGGGA GCACCTGCTT TTTGTGCGGC GGCGAAATCC CCAGATGCGG 
TACACTCTGA GTCCAGAGAA CCTCCAGAGC CTGGCTGCCC AGAGCTCCAT GCCTGAGAAC 
ATGACCCTGC AGCGGGCCAA CAGCGACACA GATCTGGTGA CTTCTGAGAG CCGGTCCAGC 
CTGACTGCCA GCATGTATGA GTACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAAG AGGAGGTGGA CCCGAGTGAT TGGATTGGAC TTTATCACAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAAAAC AGGGGTGTGA CTGGAACACA AAAAGGACAA 
ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAGC CGGAGATAAA AATCTGTTTC 
AAATACTACC ACGGCATTAG TGGAGCCCTG CGAGCCACGA CCCCCTGCAT CACGGTGAAG 
AACCCTGCTG TGATGATGGG GGCAGAAGGC ATGGAAGGAG GTGCTGCAGG CAGTCAGCAT 
TCTCGGAAAC TCGTTAGCTT TACGTTGTCA GATCTTAGGG CAGTTGGGCT AAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTGAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT 
TTCCCCACCT GTGCCCACCA TGGGCAGGAG AGGAGGTCTA CCATCATCAG TAACACCACT 
AATCCAATCT GGCACCGAGA GAAATACTCC TTTTTTGCAC TCCTTACTGA TGTCTTAGAA 
ATTGAAATTA AAGACAAATT TGCCAAGAGC CGTCCTATCA TCAAGCGTTT TCTGGGGAAA 
CTAACCATCC CAGTCCAGAG GCTGTTGGAG CGACAAGCCA TCGGTGATCA GATGCTCAGC 
TACAACCTTG GCAGAAGGCT CCCAGCTGAC CACGTGAGTG GGTACCTCCA GTTCAGAGTG 
GAGGTCACGT CTTCTGTGCA TGAAGATGCT TCTCCAGAAG CAGTTGGCAC AATACTTGGA 
GTCAATACTG TGAATGGAGA CCTAGGAAGC CCTTCTGATG AGGAGGACAT GCCAGGGGGC 
CACCACGACA GCCCGGTTTG TTCTAATGGA CCAGTTTCTG AGGAAAGTGC TGATGGGACC 
CCCAAGCATT CTTTCAGGAC TAGCTCTACT CTGGAAATAG ACACGGAAGA TTTAACCTCT 
ACTTCTTCAA GGACCTCACC TCCCAGAGGA CGTCAGGATT CCCTCAACGA TTATTTAGAT 
GCTATTGAAC ACAATGGCCA TTCCAGGTCA GGTGCAGCTA CCTGCTCAGA GAGGTCCATG 
GGAGCCTCTC CAAAACTAAG GAGTAGTTTT CCCACCGACA CGAGACTCAA TGCCATGCTT 
CATATTGATT CAGATGAGGA AGACCACGAG TTCCAGCAGG ACCTGGGTTA TCCATCTTCC 
TTGGAGGAGG AAGGAGGGTT GATAATGTTT AGCAGGGCAT CAAGAACTGA TGACGGGAGC 
CTCACATCTC AGACAAAGCC GGAAGACATC CCAGTGGAGA CTGAGGAAGC CTCTATCCAC 
GAACCTGCTT CCTTTGAGGA TAAGCCAGAA CATCTTCCGG AGCTTACAGA GAGCTCCTTA 
CCCTCAGGCC CCATGCCAGA TGAAAGTGAA AATGGCCTGG AGTCTCAACC AGGTGCCGAC 
CAGGGCAGCA CTGAATTGTG TGGCTCTCAG GAGGTCGATC AGCCTACGAG TGGGGCAGAC 
ACGGGCACTT CTGACACGTC GGGAGGAAGC AGAAGAGCCA TCAGTGAGAC AGAGTCCCTT 
GATCAGGGGT CCGAGCCTTC CCAGGTGTCC TCTGAAACAG AACCCAGTGA CCCTGCCAGG 
ACAGAGAGCG TGAGTGAGGC CAGCACCAGG CCTGAGGGAG AGAGTGACCT GGAAGGGGCC 
GACAGCTCCT GCAATGAGAG TGTCACCACG CAGCTGTCCT CGGTGGAGAC ACGGTGCTCA 
TCTCTTGAGA GTGCACGGTT TCCCGAAACC CCAGCCTTTT CTTCTCAGGA GGAGGAAGAT 
GGAGCCTGTG CAGCAGAGCC CACCAGCAGT GGCCCTGCCG AGGGGTCACA GGATTCCATA 
TGCACTCCTG GGTCCTTACC TGTGGTGCAG GTGCCCAGTG GAGAGGAGGA AGGGCCAGGG 
GCAGAATCGG CCACCTTACC TGACCAGGAG GAGGTGGGGG AGGTCTGGCA GCGGAGGGGG 
AGCCTGGAGG GAGCTGCTGC TGCTGCTGCT GCTGCTGAGA GCCAGCCCCA AGAAGAGGGT 
GATGCTGGGG AAGCCCAAGG GGCTTGTGAA GGGGCCACTG CCCAGGAGGA GGGTGCTACT 
GGAGGTTCTC AAGCCAACGG CCACCAGCCA CTGCGATCTC TGCCTTCAGT GCGCCAAGAT 
GTTAGCCGGT ACCAGAGGGT GGACGAGGCT CTCCCACCAA ACTGGGAGGC CCGCATTGAC 
AGCCACGGCA GGATCTTCTA CGTGGATCAC GTGAACAGAA CCACGACGTG GCAGCGGCCC 
ACCGCTCCCC CCGCCCCGCA GGTGCTGCAG AGGTCCAACT CCATACAGCA GATGGAGCAG 
CTGAACCGGC GATACCAGAG TATCCGCAGA ACCATGACTA ACGAGAGGCC CGAGGAAAAT 
AGCAGTGCTC TTGATGGGGC AGGAGAGGAG GCCGACTTTC ACCAAGCCAG TGCAGATTTC 
CGACGGGAGA GCGTCCTGCC CCACTCTACC TCCAGATCCA GGCTCACGTT GCTGTTACAG 
TCGCCCCCTG TGAAGTTCCT CATCAGCCCA GAGTTCTTCA CCGTGCTGCA TTCGAACCCT 
AGTGCCTACC GCATGTTTAC AAACAACACG TGTTTGAAGC ACATGATCAC CAAAGTCCGG 
CGGGACACCC ACCACTTTGA ACGCTACCAG CATAACCGGG ACCTTGTGGG ATTCCTCAAC 
ATGTTTGCAA ACAAACAACT GGAGCTGCCG AGGGGCTGGG AAATGAAACA CGATCATCAG 
GGCAAGGCAT TTTTTGTTGA CCACAACTCC CGGACCACCA CTTTCATTGA CCCCCGGCTC 
CCACTCCAGA GCAGCAGACC CACGAGTGCG CTGGTGCATC GACAGCATCT GACGAGGCAG 
CGCAGCCACA GTGCAGGCGA GGTGGGAGAA GATTCTCGAC ATGCCGGACC ACCAGTTCTT 
CCCAGGCCGT CGAGTACGTT CAACACAGTC AGCAGGCCGC AGTACCAGGA CATGGTTCCA 
GTGGCTTACA ATGACAAGAT TGTTGCATTT CTTCGCCAAC CCAACATCTT TGAAATTCTA 
CAGGAGCGTC AACCTGATCT TACCAGGAAT CACTCACTCA GGGAGAAGAT CCAATTTATC 
CGAACTGAAG GGACCCCAGG ATTGGTGCGT CTTTCAAGTG ATGCAGACCT TGTTATGTTG 
CTAAGTTTAT TTGAAGAAGA GATAATGTCA TACGTGCCTC CACATGCCTT ACTCCATCCC 
AGCTACTGTC AGTCCCCACG TGGCTCCCCT GTGTCCTCTC CCCAGAACTC GCCAGGTACT 
CAGCGTGCCA ACGCCCGGGC TCCCGCCCCT TACAAGCGAG ACTTTGAAGC CAAACTGAGG 
AATTTTTACA GGAAGTTAGA GACTAAAGGA TATGGACAAG GCCCAGGGAA ATTAAAGTTA 
ATTATCAGAA GAGATCACTT GCTCGAAGAT GCTTTTAATC AGATTATGGG CTATTCCAGA 
AAAGACCTGC AGAGAAATAA GCTGTACGTC ACATTTGTCG GGGAGGAAGG GCTGGATTAC 
AGTGGACCTT CCAGAGAGTT TTTCTTCCTG GTATCCAGAG AGCTCTTTAA CCCATATTAC 
GGCTTATTTG AATATTCAGC CAATGACACA TACACAGTAC AAATAAGTCC CATGTCTGCT 
TTTGTAGACA ATCACCATGA ATGGTTCCGG TTCAGTGGTA GGATCCTTGG TCTTGCGCTG 
ATTCACCAGT ATTTGTTGGA TGCCTTCTTC ACGCGGCCCT TCTATAAGGC TCTTCTCAGA 
ATTCTGTGTG ACCTGAGTGA TCTAGAATAC CTTGATGAGG AGTTCCACCA GAGCCTGCAG 
TGGATGAAAG ACAATGATAT CCATGACATT CTGGACCTCA CGTTCACAGT GAACGAAGAA 
GTTTTCGGGC AGATAACTGA ACGAGAATTA AAACCAGGGG GTGCCAACAT CCCAGTCACA 
GAGAAGAACA AGAAGGAGTA CATCGAGAGG ATGGTGAAAT GGAGGATTGA GCGGGGTGTT 
GTGCAGCAAA CGGAGAGCTT AGTGCGTGGC TTCTACGAGG TGGTGGATGC CAGGCTAGTA 
TCTGTTTTTG ATGCAAGAGA ACTGGAACTG GTCATCGCAG GCACAGCTGA AATAGACCTA 
AGTGATTGGA GAAACAACAC AGAATACAGA GGAGGTTATC ATGACAATCA TATTGTAATT 
CGGTGGTTCT GGGCTGCAGT GGAGAGATTC AACAATGAAC AACGATTAAG GTTGTTACAG 
TTTGTCACAG GCACATCCAG CATTCCCTAT GAAGGATTTG CTTCTCTCCG AGGGAGTAAT 
GGCCCAAGAA GATTCTGCGT GGAGAAATGG GGGAAAATCA CCGCTCTTCC CAGAGCTCAC 
ACTTGTTTTA ACCGTCTGGA TCTCCCTCCA TACCCATCCT TCTCCATGCT TTATGAAAAA 
CTTTTGACAG CAGTTGAGGA GACCAGTACC TTTGGACTTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020930200.1
CDS	182..4906
Translation	MASSAREHLLFVRRRNPQMRYTLSPENLQSLAAQSSMPENMTLQRANSDTDLVTSESRSSLTASMYEYTLGQAQNLIIFWDIKEEVDPSDWIGLYHIDENSPANFWDSKNRGVTGTQKGQIVWRIEPGPYFMEPEIKICFKYYHGISGALRATTPCITVKNPAVMMGAEGMEGGAAGSQHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTCAHHGQERRSTIISNTTNPIWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAIGDQMLSYNLGRRLPADHVSGYLQFRVEVTSSVHEDASPEAVGTILGVNTVNGDLGSPSDEEDMPGGHHDSPVCSNGPVSEESADGTPKHSFRTSSTLEIDTEDLTSTSSRTSPPRGRQDSLNDYLDAIEHNGHSRSGAATCSERSMGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMFSRASRTDDGSLTSQTKPEDIPVETEEASIHEPASFEDKPEHLPELTESSLPSGPMPDESENGLESQPGADQGSTELCGSQEVDQPTSGADTGTSDTSGGSRRAISETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDLEGADSSCNESVTTQLSSVETRCSSLESARFPETPAFSSQEEEDGACAAEPTSSGPAEGSQDSICTPGSLPVVQVPSGEEEGPGAESATLPDQEEVGEVWQRRGSLEGAAAAAAAAESQPQEEGDAGEAQGACEGATAQEEGATGGSQANGHQPLRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENSSALDGAGEEADFHQASADFRRESVLPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNIFEILQERQPDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_021074541.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021074541.1

ORF Insert Sequence:

ATGGCTAGCT CAGCCCGGGA GCACCTGCTT TTTGTGCGGC GGCGAAATCC CCAGATGCGG 
TACACTCTGA GTCCAGAGAA CCTCCAGAGC CTGGCTGCCC AGAGCTCCAT GCCTGAGAAC 
ATGACCCTGC AGCGGGCCAA CAGCGACACA GATCTGGTGA CTTCTGAGAG CCGGTCCAGC 
CTGACTGCCA GCATGTATGA GTACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAAG AGGAGGTGGA CCCGAGTGAT TGGATTGGAC TTTATCACAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAAAAC AGGGGTGTGA CTGGAACACA AAAAGGACAA 
ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAGC CGGAGATAAA AATCTGTTTC 
AAATACTACC ACGGCATTAG TGGAGCCCTG CGAGCCACGA CCCCCTGCAT CACGGTGAAG 
AACCCTGCTG TGATGATGGG GGCAGAAGGC ATGGAAGGAG GTGCTGCAGG CAGTCAGCAT 
TCTCGGAAAC TCGTTAGCTT TACGTTGTCA GATCTTAGGG CAGTTGGGCT AAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTGAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT 
TTCCCCACCT GTGCCCACCA TGGGCAGGAG AGGAGGTCTA CCATCATCAG TAACACCACT 
AATCCAATCT GGCACCGAGA GAAATACTCC TTTTTTGCAC TCCTTACTGA TGTCTTAGAA 
ATTGAAATTA AAGACAAATT TGCCAAGAGC CGTCCTATCA TCAAGCGTTT TCTGGGGAAA 
CTAACCATCC CAGTCCAGAG GCTGTTGGAG CGACAAGCCA TCGGTGATCA GATGCTCAGC 
TACAACCTTG GCAGAAGGCT CCCAGCTGAC CACGTGAGTG GGTACCTCCA GTTCAGAGTG 
GAGGTCACGT CTTCTGTGCA TGAAGATGCT TCTCCAGAAG CAGTTGGCAC AATACTTGGA 
GTCAATACTG TGAATGGAGA CCTAGGAAGC CCTTCTGATG AGGAGGACAT GCCAGGGGGC 
CACCACGACA GCCCGGTTTG TTCTAATGGA CCAGTTTCTG AGGAAAGTGC TGATGGGACC 
CCCAAGCATT CTTTCAGGAC TAGCTCTACT CTGGAAATAG ACACGGAAGA TTTAACCTCT 
ACTTCTTCAA GGACCTCACC TCCCAGAGGA CGTCAGGATT CCCTCAACGA TTATTTAGAT 
GCTATTGAAC ACAATGGCCA TTCCAGGTCA GGTGCAGCTA CCTGCTCAGA GAGGTCCATG 
GGAGCCTCTC CAAAACTAAG GAGTAGTTTT CCCACCGACA CGAGACTCAA TGCCATGCTT 
CATATTGATT CAGATGAGGA AGACCACGAG TTCCAGCAGG ACCTGGGTTA TCCATCTTCC 
TTGGAGGAGG AAGGAGGGTT GATAATGTTT AGCAGGGCAT CAAGAACTGA TGACGGGAGC 
CTCACATCTC AGACAAAGCC GGAAGACATC CCAGTGGAGA CTGAGGAAGC CTCTATCCAC 
GAACCTGCTT CCTTTGAGGA TAAGCCAGAA CATCTTCCGG AGCTTACAGA GAGCTCCTTA 
CCCTCAGGCC CCATGCCAGA TGAAAGTGAA AATGGCCTGG AGTCTCAACC AGGTGCCGAC 
CAGGGCAGCA CTGAATTGTG TGGCTCTCAG GAGGTCGATC AGCCTACGAG TGGGGCAGAC 
ACGGGCACTT CTGACACGTC GGGAGGAAGC AGAAGAGCCA TCAGTGAGAC AGAGTCCCTT 
GATCAGGGGT CCGAGCCTTC CCAGGTGTCC TCTGAAACAG AACCCAGTGA CCCTGCCAGG 
ACAGAGAGCG TGAGTGAGGC CAGCACCAGG CCTGAGGGAG AGAGTGACCT GGAAGGGGCC 
GACAGCTCCT GCAATGAGAG TGTCACCACG CAGCTGTCCT CGGTGGAGAC ACGGTGCTCA 
TCTCTTGAGA GTGCACGGTT TCCCGAAACC CCAGCCTTTT CTTCTCAGGA GGAGGAAGAT 
GGAGCCTGTG CAGCAGAGCC CACCAGCAGT GGCCCTGCCG AGGGGTCACA GGATTCCATA 
TGCACTCCTG GGTCCTTACC TGTGGTGCAG GTGCCCAGTG GAGAGGAGGA AGGGCCAGGG 
GCAGAATCGG CCACCTTACC TGACCAGGAG GAGGTGGGGG AGGTCTGGCA GCGGAGGGGG 
AGCCTGGAGG GAGCTGCTGC TGCTGCTGCT GCTGCTGAGA GCCAGCCCCA AGAAGAGGGT 
GATGCTGGGG AAGCCCAAGG GGCTTGTGAA GGGGCCACTG CCCAGGAGGA GGGTGCTACT 
GGAGGTTCTC AAGCCAACGG CCACCAGCCA CTGCGATCTC TGCCTTCAGT GCGCCAAGAT 
GTTAGCCGGT ACCAGAGGGT GGACGAGGCT CTCCCACCAA ACTGGGAGGC CCGCATTGAC 
AGCCACGGCA GGATCTTCTA CGTGGATCAC GTGAACAGAA CCACGACGTG GCAGCGGCCC 
ACCGCTCCCC CCGCCCCGCA GGTGCTGCAG AGGTCCAACT CCATACAGCA GATGGAGCAG 
CTGAACCGGC GATACCAGAG TATCCGCAGA ACCATGACTA ACGAGAGGCC CGAGGAAAAT 
AGCAGTGCTC TTGATGGGGC AGGAGAGGAG GCCGACTTTC ACCAAGCCAG TGCAGATTTC 
CGACGGGAGA GCGTCCTGCC CCACTCTACC TCCAGATCCA GGCTCACGTT GCTGTTACAG 
TCGCCCCCTG TGAAGTTCCT CATCAGCCCA GAGTTCTTCA CCGTGCTGCA TTCGAACCCT 
AGTGCCTACC GCATGTTTAC AAACAACACG TGTTTGAAGC ACATGATCAC CAAAGTCCGG 
CGGGACACCC ACCACTTTGA ACGCTACCAG CATAACCGGG ACCTTGTGGG ATTCCTCAAC 
ATGTTTGCAA ACAAACAACT GGAGCTGCCG AGGGGCTGGG AAATGAAACA CGATCATCAG 
GGCAAGGCAT TTTTTGTTGA CCACAACTCC CGGACCACCA CTTTCATTGA CCCCCGGCTC 
CCACTCCAGA GCAGCAGACC CACGAGTGCG CTGGTGCATC GACAGCATCT GACGAGGCAG 
CGCAGCCACA GTGCAGGCGA GGTGGGAGAA GATTCTCGAC ATGCCGGACC ACCAGTTCTT 
CCCAGGCCGT CGAGTACGTT CAACACAGTC AGCAGGCCGC AGTACCAGGA CATGGTTCCA 
GTGGCTTACA ATGACAAGAT TGTTGCATTT CTTCGCCAAC CCAACATCTT TGAAATTCTA 
CAGGAGCGTC AACCTGATCT TACCAGGAAT CACTCACTCA GGGAGAAGAT CCAATTTATC 
CGAACTGAAG GGACCCCAGG ATTGGTGCGT CTTTCAAGTG ATGCAGACCT TGTTATGTTG 
CTAAGTTTAT TTGAAGAAGA GATAATGTCA TACGTGCCTC CACATGCCTT ACTCCATCCC 
AGCTACTGTC AGTCCCCACG TGGCTCCCCT GTGTCCTCTC CCCAGAACTC GCCAGGTACT 
CAGCGTGCCA ACGCCCGGGC TCCCGCCCCT TACAAGCGAG ACTTTGAAGC CAAACTGAGG 
AATTTTTACA GGAAGTTAGA GACTAAAGGA TATGGACAAG GCCCAGGGAA ATTAAAGTTA 
ATTATCAGAA GAGATCACTT GCTCGAAGAT GCTTTTAATC AGATTATGGG CTATTCCAGA 
AAAGACCTGC AGAGAAATAA GCTGTACGTC ACATTTGTCG GGGAGGAAGG GCTGGATTAC 
AGTGGACCTT CCAGAGAGTT TTTCTTCCTG GTATCCAGAG AGCTCTTTAA CCCATATTAC 
GGCTTATTTG AATATTCAGC CAATGACACA TACACAGTAC AAATAAGTCC CATGTCTGCT 
TTTGTAGACA ATCACCATGA ATGGTTCCGG TTCAGTGGTA GGATCCTTGG TCTTGCGCTG 
ATTCACCAGT ATTTGTTGGA TGCCTTCTTC ACGCGGCCCT TCTATAAGGC TCTTCTCAGA 
ATTCTGTGTG ACCTGAGTGA TCTAGAATAC CTTGATGAGG AGTTCCACCA GAGCCTGCAG 
TGGATGAAAG ACAATGATAT CCATGACATT CTGGACCTCA CGTTCACAGT GAACGAAGAA 
GTTTTCGGGC AGATAACTGA ACGAGAATTA AAACCAGGGG GTGCCAACAT CCCAGTCACA 
GAGAAGAACA AGAAGGAGTA CATCGAGAGG ATGGTGAAAT GGAGGATTGA GCGGGGTGTT 
GTGCAGCAAA CGGAGAGCTT AGTGCGTGGC TTCTACGAGG TGGTGGATGC CAGGCTAGTA 
TCTGTTTTTG ATGCAAGAGA ACTGGAACTG GTCATCGCAG GCACAGCTGA AATAGACCTA 
AGTGATTGGA GAAACAACAC AGAATACAGA GGAGGTTATC ATGACAATCA TATTGTAATT 
CGGTGGTTCT GGGCTGCAGT GGAGAGATTC AACAATGAAC AACGATTAAG GTTGTTACAG 
TTTGTCACAG GCACATCCAG CATTCCCTAT GAAGGATTTG CTTCTCTCCG AGGGAGTAAT 
GGCCCAAGAA GATTCTGCGT GGAGAAATGG GGGAAAATCA CCGCTCTTCC CAGAGCTCAC 
ACTTGTTTTA ACCGTCTGGA TCTCCCTCCA TACCCATCCT TCTCCATGCT TTATGAAAAA 
CTTTTGACAG CAGTTGAGGA GACCAGTACC TTTGGACTTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW2 ( XM_021074543.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020930202.1 Sus scrofa HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X3, mRNA.

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CloneID	OSe01255
Clone ID Related Accession (Same CDS sequence)	XM_021074541.1 , XM_021074543.1 , NM_001198924.1 , XM_021074542.1 , XM_021074545.1 , XM_021074544.1
Accession Version	XM_021074543.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Sus scrofa(pig)
Product	E3 ubiquitin-protein ligase HECW2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010457.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAGCT CAGCCCGGGA GCACCTGCTT TTTGTGCGGC GGCGAAATCC CCAGATGCGG 
TACACTCTGA GTCCAGAGAA CCTCCAGAGC CTGGCTGCCC AGAGCTCCAT GCCTGAGAAC 
ATGACCCTGC AGCGGGCCAA CAGCGACACA GATCTGGTGA CTTCTGAGAG CCGGTCCAGC 
CTGACTGCCA GCATGTATGA GTACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAAG AGGAGGTGGA CCCGAGTGAT TGGATTGGAC TTTATCACAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAAAAC AGGGGTGTGA CTGGAACACA AAAAGGACAA 
ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAGC CGGAGATAAA AATCTGTTTC 
AAATACTACC ACGGCATTAG TGGAGCCCTG CGAGCCACGA CCCCCTGCAT CACGGTGAAG 
AACCCTGCTG TGATGATGGG GGCAGAAGGC ATGGAAGGAG GTGCTGCAGG CAGTCAGCAT 
TCTCGGAAAC TCGTTAGCTT TACGTTGTCA GATCTTAGGG CAGTTGGGCT AAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTGAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT 
TTCCCCACCT GTGCCCACCA TGGGCAGGAG AGGAGGTCTA CCATCATCAG TAACACCACT 
AATCCAATCT GGCACCGAGA GAAATACTCC TTTTTTGCAC TCCTTACTGA TGTCTTAGAA 
ATTGAAATTA AAGACAAATT TGCCAAGAGC CGTCCTATCA TCAAGCGTTT TCTGGGGAAA 
CTAACCATCC CAGTCCAGAG GCTGTTGGAG CGACAAGCCA TCGGTGATCA GATGCTCAGC 
TACAACCTTG GCAGAAGGCT CCCAGCTGAC CACGTGAGTG GGTACCTCCA GTTCAGAGTG 
GAGGTCACGT CTTCTGTGCA TGAAGATGCT TCTCCAGAAG CAGTTGGCAC AATACTTGGA 
GTCAATACTG TGAATGGAGA CCTAGGAAGC CCTTCTGATG AGGAGGACAT GCCAGGGGGC 
CACCACGACA GCCCGGTTTG TTCTAATGGA CCAGTTTCTG AGGAAAGTGC TGATGGGACC 
CCCAAGCATT CTTTCAGGAC TAGCTCTACT CTGGAAATAG ACACGGAAGA TTTAACCTCT 
ACTTCTTCAA GGACCTCACC TCCCAGAGGA CGTCAGGATT CCCTCAACGA TTATTTAGAT 
GCTATTGAAC ACAATGGCCA TTCCAGGTCA GGTGCAGCTA CCTGCTCAGA GAGGTCCATG 
GGAGCCTCTC CAAAACTAAG GAGTAGTTTT CCCACCGACA CGAGACTCAA TGCCATGCTT 
CATATTGATT CAGATGAGGA AGACCACGAG TTCCAGCAGG ACCTGGGTTA TCCATCTTCC 
TTGGAGGAGG AAGGAGGGTT GATAATGTTT AGCAGGGCAT CAAGAACTGA TGACGGGAGC 
CTCACATCTC AGACAAAGCC GGAAGACATC CCAGTGGAGA CTGAGGAAGC CTCTATCCAC 
GAACCTGCTT CCTTTGAGGA TAAGCCAGAA CATCTTCCGG AGCTTACAGA GAGCTCCTTA 
CCCTCAGGCC CCATGCCAGA TGAAAGTGAA AATGGCCTGG AGTCTCAACC AGGTGCCGAC 
CAGGGCAGCA CTGAATTGTG TGGCTCTCAG GAGGTCGATC AGCCTACGAG TGGGGCAGAC 
ACGGGCACTT CTGACACGTC GGGAGGAAGC AGAAGAGCCA TCAGTGAGAC AGAGTCCCTT 
GATCAGGGGT CCGAGCCTTC CCAGGTGTCC TCTGAAACAG AACCCAGTGA CCCTGCCAGG 
ACAGAGAGCG TGAGTGAGGC CAGCACCAGG CCTGAGGGAG AGAGTGACCT GGAAGGGGCC 
GACAGCTCCT GCAATGAGAG TGTCACCACG CAGCTGTCCT CGGTGGAGAC ACGGTGCTCA 
TCTCTTGAGA GTGCACGGTT TCCCGAAACC CCAGCCTTTT CTTCTCAGGA GGAGGAAGAT 
GGAGCCTGTG CAGCAGAGCC CACCAGCAGT GGCCCTGCCG AGGGGTCACA GGATTCCATA 
TGCACTCCTG GGTCCTTACC TGTGGTGCAG GTGCCCAGTG GAGAGGAGGA AGGGCCAGGG 
GCAGAATCGG CCACCTTACC TGACCAGGAG GAGGTGGGGG AGGTCTGGCA GCGGAGGGGG 
AGCCTGGAGG GAGCTGCTGC TGCTGCTGCT GCTGCTGAGA GCCAGCCCCA AGAAGAGGGT 
GATGCTGGGG AAGCCCAAGG GGCTTGTGAA GGGGCCACTG CCCAGGAGGA GGGTGCTACT 
GGAGGTTCTC AAGCCAACGG CCACCAGCCA CTGCGATCTC TGCCTTCAGT GCGCCAAGAT 
GTTAGCCGGT ACCAGAGGGT GGACGAGGCT CTCCCACCAA ACTGGGAGGC CCGCATTGAC 
AGCCACGGCA GGATCTTCTA CGTGGATCAC GTGAACAGAA CCACGACGTG GCAGCGGCCC 
ACCGCTCCCC CCGCCCCGCA GGTGCTGCAG AGGTCCAACT CCATACAGCA GATGGAGCAG 
CTGAACCGGC GATACCAGAG TATCCGCAGA ACCATGACTA ACGAGAGGCC CGAGGAAAAT 
AGCAGTGCTC TTGATGGGGC AGGAGAGGAG GCCGACTTTC ACCAAGCCAG TGCAGATTTC 
CGACGGGAGA GCGTCCTGCC CCACTCTACC TCCAGATCCA GGCTCACGTT GCTGTTACAG 
TCGCCCCCTG TGAAGTTCCT CATCAGCCCA GAGTTCTTCA CCGTGCTGCA TTCGAACCCT 
AGTGCCTACC GCATGTTTAC AAACAACACG TGTTTGAAGC ACATGATCAC CAAAGTCCGG 
CGGGACACCC ACCACTTTGA ACGCTACCAG CATAACCGGG ACCTTGTGGG ATTCCTCAAC 
ATGTTTGCAA ACAAACAACT GGAGCTGCCG AGGGGCTGGG AAATGAAACA CGATCATCAG 
GGCAAGGCAT TTTTTGTTGA CCACAACTCC CGGACCACCA CTTTCATTGA CCCCCGGCTC 
CCACTCCAGA GCAGCAGACC CACGAGTGCG CTGGTGCATC GACAGCATCT GACGAGGCAG 
CGCAGCCACA GTGCAGGCGA GGTGGGAGAA GATTCTCGAC ATGCCGGACC ACCAGTTCTT 
CCCAGGCCGT CGAGTACGTT CAACACAGTC AGCAGGCCGC AGTACCAGGA CATGGTTCCA 
GTGGCTTACA ATGACAAGAT TGTTGCATTT CTTCGCCAAC CCAACATCTT TGAAATTCTA 
CAGGAGCGTC AACCTGATCT TACCAGGAAT CACTCACTCA GGGAGAAGAT CCAATTTATC 
CGAACTGAAG GGACCCCAGG ATTGGTGCGT CTTTCAAGTG ATGCAGACCT TGTTATGTTG 
CTAAGTTTAT TTGAAGAAGA GATAATGTCA TACGTGCCTC CACATGCCTT ACTCCATCCC 
AGCTACTGTC AGTCCCCACG TGGCTCCCCT GTGTCCTCTC CCCAGAACTC GCCAGGTACT 
CAGCGTGCCA ACGCCCGGGC TCCCGCCCCT TACAAGCGAG ACTTTGAAGC CAAACTGAGG 
AATTTTTACA GGAAGTTAGA GACTAAAGGA TATGGACAAG GCCCAGGGAA ATTAAAGTTA 
ATTATCAGAA GAGATCACTT GCTCGAAGAT GCTTTTAATC AGATTATGGG CTATTCCAGA 
AAAGACCTGC AGAGAAATAA GCTGTACGTC ACATTTGTCG GGGAGGAAGG GCTGGATTAC 
AGTGGACCTT CCAGAGAGTT TTTCTTCCTG GTATCCAGAG AGCTCTTTAA CCCATATTAC 
GGCTTATTTG AATATTCAGC CAATGACACA TACACAGTAC AAATAAGTCC CATGTCTGCT 
TTTGTAGACA ATCACCATGA ATGGTTCCGG TTCAGTGGTA GGATCCTTGG TCTTGCGCTG 
ATTCACCAGT ATTTGTTGGA TGCCTTCTTC ACGCGGCCCT TCTATAAGGC TCTTCTCAGA 
ATTCTGTGTG ACCTGAGTGA TCTAGAATAC CTTGATGAGG AGTTCCACCA GAGCCTGCAG 
TGGATGAAAG ACAATGATAT CCATGACATT CTGGACCTCA CGTTCACAGT GAACGAAGAA 
GTTTTCGGGC AGATAACTGA ACGAGAATTA AAACCAGGGG GTGCCAACAT CCCAGTCACA 
GAGAAGAACA AGAAGGAGTA CATCGAGAGG ATGGTGAAAT GGAGGATTGA GCGGGGTGTT 
GTGCAGCAAA CGGAGAGCTT AGTGCGTGGC TTCTACGAGG TGGTGGATGC CAGGCTAGTA 
TCTGTTTTTG ATGCAAGAGA ACTGGAACTG GTCATCGCAG GCACAGCTGA AATAGACCTA 
AGTGATTGGA GAAACAACAC AGAATACAGA GGAGGTTATC ATGACAATCA TATTGTAATT 
CGGTGGTTCT GGGCTGCAGT GGAGAGATTC AACAATGAAC AACGATTAAG GTTGTTACAG 
TTTGTCACAG GCACATCCAG CATTCCCTAT GAAGGATTTG CTTCTCTCCG AGGGAGTAAT 
GGCCCAAGAA GATTCTGCGT GGAGAAATGG GGGAAAATCA CCGCTCTTCC CAGAGCTCAC 
ACTTGTTTTA ACCGTCTGGA TCTCCCTCCA TACCCATCCT TCTCCATGCT TTATGAAAAA 
CTTTTGACAG CAGTTGAGGA GACCAGTACC TTTGGACTTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020930202.1
CDS	158..4882
Translation	MASSAREHLLFVRRRNPQMRYTLSPENLQSLAAQSSMPENMTLQRANSDTDLVTSESRSSLTASMYEYTLGQAQNLIIFWDIKEEVDPSDWIGLYHIDENSPANFWDSKNRGVTGTQKGQIVWRIEPGPYFMEPEIKICFKYYHGISGALRATTPCITVKNPAVMMGAEGMEGGAAGSQHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTCAHHGQERRSTIISNTTNPIWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAIGDQMLSYNLGRRLPADHVSGYLQFRVEVTSSVHEDASPEAVGTILGVNTVNGDLGSPSDEEDMPGGHHDSPVCSNGPVSEESADGTPKHSFRTSSTLEIDTEDLTSTSSRTSPPRGRQDSLNDYLDAIEHNGHSRSGAATCSERSMGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMFSRASRTDDGSLTSQTKPEDIPVETEEASIHEPASFEDKPEHLPELTESSLPSGPMPDESENGLESQPGADQGSTELCGSQEVDQPTSGADTGTSDTSGGSRRAISETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDLEGADSSCNESVTTQLSSVETRCSSLESARFPETPAFSSQEEEDGACAAEPTSSGPAEGSQDSICTPGSLPVVQVPSGEEEGPGAESATLPDQEEVGEVWQRRGSLEGAAAAAAAAESQPQEEGDAGEAQGACEGATAQEEGATGGSQANGHQPLRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENSSALDGAGEEADFHQASADFRRESVLPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNIFEILQERQPDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_021074543.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021074543.1

ORF Insert Sequence:

ATGGCTAGCT CAGCCCGGGA GCACCTGCTT TTTGTGCGGC GGCGAAATCC CCAGATGCGG 
TACACTCTGA GTCCAGAGAA CCTCCAGAGC CTGGCTGCCC AGAGCTCCAT GCCTGAGAAC 
ATGACCCTGC AGCGGGCCAA CAGCGACACA GATCTGGTGA CTTCTGAGAG CCGGTCCAGC 
CTGACTGCCA GCATGTATGA GTACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAAG AGGAGGTGGA CCCGAGTGAT TGGATTGGAC TTTATCACAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAAAAC AGGGGTGTGA CTGGAACACA AAAAGGACAA 
ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAGC CGGAGATAAA AATCTGTTTC 
AAATACTACC ACGGCATTAG TGGAGCCCTG CGAGCCACGA CCCCCTGCAT CACGGTGAAG 
AACCCTGCTG TGATGATGGG GGCAGAAGGC ATGGAAGGAG GTGCTGCAGG CAGTCAGCAT 
TCTCGGAAAC TCGTTAGCTT TACGTTGTCA GATCTTAGGG CAGTTGGGCT AAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTGAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT 
TTCCCCACCT GTGCCCACCA TGGGCAGGAG AGGAGGTCTA CCATCATCAG TAACACCACT 
AATCCAATCT GGCACCGAGA GAAATACTCC TTTTTTGCAC TCCTTACTGA TGTCTTAGAA 
ATTGAAATTA AAGACAAATT TGCCAAGAGC CGTCCTATCA TCAAGCGTTT TCTGGGGAAA 
CTAACCATCC CAGTCCAGAG GCTGTTGGAG CGACAAGCCA TCGGTGATCA GATGCTCAGC 
TACAACCTTG GCAGAAGGCT CCCAGCTGAC CACGTGAGTG GGTACCTCCA GTTCAGAGTG 
GAGGTCACGT CTTCTGTGCA TGAAGATGCT TCTCCAGAAG CAGTTGGCAC AATACTTGGA 
GTCAATACTG TGAATGGAGA CCTAGGAAGC CCTTCTGATG AGGAGGACAT GCCAGGGGGC 
CACCACGACA GCCCGGTTTG TTCTAATGGA CCAGTTTCTG AGGAAAGTGC TGATGGGACC 
CCCAAGCATT CTTTCAGGAC TAGCTCTACT CTGGAAATAG ACACGGAAGA TTTAACCTCT 
ACTTCTTCAA GGACCTCACC TCCCAGAGGA CGTCAGGATT CCCTCAACGA TTATTTAGAT 
GCTATTGAAC ACAATGGCCA TTCCAGGTCA GGTGCAGCTA CCTGCTCAGA GAGGTCCATG 
GGAGCCTCTC CAAAACTAAG GAGTAGTTTT CCCACCGACA CGAGACTCAA TGCCATGCTT 
CATATTGATT CAGATGAGGA AGACCACGAG TTCCAGCAGG ACCTGGGTTA TCCATCTTCC 
TTGGAGGAGG AAGGAGGGTT GATAATGTTT AGCAGGGCAT CAAGAACTGA TGACGGGAGC 
CTCACATCTC AGACAAAGCC GGAAGACATC CCAGTGGAGA CTGAGGAAGC CTCTATCCAC 
GAACCTGCTT CCTTTGAGGA TAAGCCAGAA CATCTTCCGG AGCTTACAGA GAGCTCCTTA 
CCCTCAGGCC CCATGCCAGA TGAAAGTGAA AATGGCCTGG AGTCTCAACC AGGTGCCGAC 
CAGGGCAGCA CTGAATTGTG TGGCTCTCAG GAGGTCGATC AGCCTACGAG TGGGGCAGAC 
ACGGGCACTT CTGACACGTC GGGAGGAAGC AGAAGAGCCA TCAGTGAGAC AGAGTCCCTT 
GATCAGGGGT CCGAGCCTTC CCAGGTGTCC TCTGAAACAG AACCCAGTGA CCCTGCCAGG 
ACAGAGAGCG TGAGTGAGGC CAGCACCAGG CCTGAGGGAG AGAGTGACCT GGAAGGGGCC 
GACAGCTCCT GCAATGAGAG TGTCACCACG CAGCTGTCCT CGGTGGAGAC ACGGTGCTCA 
TCTCTTGAGA GTGCACGGTT TCCCGAAACC CCAGCCTTTT CTTCTCAGGA GGAGGAAGAT 
GGAGCCTGTG CAGCAGAGCC CACCAGCAGT GGCCCTGCCG AGGGGTCACA GGATTCCATA 
TGCACTCCTG GGTCCTTACC TGTGGTGCAG GTGCCCAGTG GAGAGGAGGA AGGGCCAGGG 
GCAGAATCGG CCACCTTACC TGACCAGGAG GAGGTGGGGG AGGTCTGGCA GCGGAGGGGG 
AGCCTGGAGG GAGCTGCTGC TGCTGCTGCT GCTGCTGAGA GCCAGCCCCA AGAAGAGGGT 
GATGCTGGGG AAGCCCAAGG GGCTTGTGAA GGGGCCACTG CCCAGGAGGA GGGTGCTACT 
GGAGGTTCTC AAGCCAACGG CCACCAGCCA CTGCGATCTC TGCCTTCAGT GCGCCAAGAT 
GTTAGCCGGT ACCAGAGGGT GGACGAGGCT CTCCCACCAA ACTGGGAGGC CCGCATTGAC 
AGCCACGGCA GGATCTTCTA CGTGGATCAC GTGAACAGAA CCACGACGTG GCAGCGGCCC 
ACCGCTCCCC CCGCCCCGCA GGTGCTGCAG AGGTCCAACT CCATACAGCA GATGGAGCAG 
CTGAACCGGC GATACCAGAG TATCCGCAGA ACCATGACTA ACGAGAGGCC CGAGGAAAAT 
AGCAGTGCTC TTGATGGGGC AGGAGAGGAG GCCGACTTTC ACCAAGCCAG TGCAGATTTC 
CGACGGGAGA GCGTCCTGCC CCACTCTACC TCCAGATCCA GGCTCACGTT GCTGTTACAG 
TCGCCCCCTG TGAAGTTCCT CATCAGCCCA GAGTTCTTCA CCGTGCTGCA TTCGAACCCT 
AGTGCCTACC GCATGTTTAC AAACAACACG TGTTTGAAGC ACATGATCAC CAAAGTCCGG 
CGGGACACCC ACCACTTTGA ACGCTACCAG CATAACCGGG ACCTTGTGGG ATTCCTCAAC 
ATGTTTGCAA ACAAACAACT GGAGCTGCCG AGGGGCTGGG AAATGAAACA CGATCATCAG 
GGCAAGGCAT TTTTTGTTGA CCACAACTCC CGGACCACCA CTTTCATTGA CCCCCGGCTC 
CCACTCCAGA GCAGCAGACC CACGAGTGCG CTGGTGCATC GACAGCATCT GACGAGGCAG 
CGCAGCCACA GTGCAGGCGA GGTGGGAGAA GATTCTCGAC ATGCCGGACC ACCAGTTCTT 
CCCAGGCCGT CGAGTACGTT CAACACAGTC AGCAGGCCGC AGTACCAGGA CATGGTTCCA 
GTGGCTTACA ATGACAAGAT TGTTGCATTT CTTCGCCAAC CCAACATCTT TGAAATTCTA 
CAGGAGCGTC AACCTGATCT TACCAGGAAT CACTCACTCA GGGAGAAGAT CCAATTTATC 
CGAACTGAAG GGACCCCAGG ATTGGTGCGT CTTTCAAGTG ATGCAGACCT TGTTATGTTG 
CTAAGTTTAT TTGAAGAAGA GATAATGTCA TACGTGCCTC CACATGCCTT ACTCCATCCC 
AGCTACTGTC AGTCCCCACG TGGCTCCCCT GTGTCCTCTC CCCAGAACTC GCCAGGTACT 
CAGCGTGCCA ACGCCCGGGC TCCCGCCCCT TACAAGCGAG ACTTTGAAGC CAAACTGAGG 
AATTTTTACA GGAAGTTAGA GACTAAAGGA TATGGACAAG GCCCAGGGAA ATTAAAGTTA 
ATTATCAGAA GAGATCACTT GCTCGAAGAT GCTTTTAATC AGATTATGGG CTATTCCAGA 
AAAGACCTGC AGAGAAATAA GCTGTACGTC ACATTTGTCG GGGAGGAAGG GCTGGATTAC 
AGTGGACCTT CCAGAGAGTT TTTCTTCCTG GTATCCAGAG AGCTCTTTAA CCCATATTAC 
GGCTTATTTG AATATTCAGC CAATGACACA TACACAGTAC AAATAAGTCC CATGTCTGCT 
TTTGTAGACA ATCACCATGA ATGGTTCCGG TTCAGTGGTA GGATCCTTGG TCTTGCGCTG 
ATTCACCAGT ATTTGTTGGA TGCCTTCTTC ACGCGGCCCT TCTATAAGGC TCTTCTCAGA 
ATTCTGTGTG ACCTGAGTGA TCTAGAATAC CTTGATGAGG AGTTCCACCA GAGCCTGCAG 
TGGATGAAAG ACAATGATAT CCATGACATT CTGGACCTCA CGTTCACAGT GAACGAAGAA 
GTTTTCGGGC AGATAACTGA ACGAGAATTA AAACCAGGGG GTGCCAACAT CCCAGTCACA 
GAGAAGAACA AGAAGGAGTA CATCGAGAGG ATGGTGAAAT GGAGGATTGA GCGGGGTGTT 
GTGCAGCAAA CGGAGAGCTT AGTGCGTGGC TTCTACGAGG TGGTGGATGC CAGGCTAGTA 
TCTGTTTTTG ATGCAAGAGA ACTGGAACTG GTCATCGCAG GCACAGCTGA AATAGACCTA 
AGTGATTGGA GAAACAACAC AGAATACAGA GGAGGTTATC ATGACAATCA TATTGTAATT 
CGGTGGTTCT GGGCTGCAGT GGAGAGATTC AACAATGAAC AACGATTAAG GTTGTTACAG 
TTTGTCACAG GCACATCCAG CATTCCCTAT GAAGGATTTG CTTCTCTCCG AGGGAGTAAT 
GGCCCAAGAA GATTCTGCGT GGAGAAATGG GGGAAAATCA CCGCTCTTCC CAGAGCTCAC 
ACTTGTTTTA ACCGTCTGGA TCTCCCTCCA TACCCATCCT TCTCCATGCT TTATGAAAAA 
CTTTTGACAG CAGTTGAGGA GACCAGTACC TTTGGACTTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW2 ( XM_021074542.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020930201.1 Sus scrofa HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X2, mRNA.

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CloneID	OSe01255
Clone ID Related Accession (Same CDS sequence)	XM_021074541.1 , XM_021074543.1 , NM_001198924.1 , XM_021074542.1 , XM_021074545.1 , XM_021074544.1
Accession Version	XM_021074542.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Sus scrofa(pig)
Product	E3 ubiquitin-protein ligase HECW2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010457.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAGCT CAGCCCGGGA GCACCTGCTT TTTGTGCGGC GGCGAAATCC CCAGATGCGG 
TACACTCTGA GTCCAGAGAA CCTCCAGAGC CTGGCTGCCC AGAGCTCCAT GCCTGAGAAC 
ATGACCCTGC AGCGGGCCAA CAGCGACACA GATCTGGTGA CTTCTGAGAG CCGGTCCAGC 
CTGACTGCCA GCATGTATGA GTACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAAG AGGAGGTGGA CCCGAGTGAT TGGATTGGAC TTTATCACAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAAAAC AGGGGTGTGA CTGGAACACA AAAAGGACAA 
ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAGC CGGAGATAAA AATCTGTTTC 
AAATACTACC ACGGCATTAG TGGAGCCCTG CGAGCCACGA CCCCCTGCAT CACGGTGAAG 
AACCCTGCTG TGATGATGGG GGCAGAAGGC ATGGAAGGAG GTGCTGCAGG CAGTCAGCAT 
TCTCGGAAAC TCGTTAGCTT TACGTTGTCA GATCTTAGGG CAGTTGGGCT AAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTGAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT 
TTCCCCACCT GTGCCCACCA TGGGCAGGAG AGGAGGTCTA CCATCATCAG TAACACCACT 
AATCCAATCT GGCACCGAGA GAAATACTCC TTTTTTGCAC TCCTTACTGA TGTCTTAGAA 
ATTGAAATTA AAGACAAATT TGCCAAGAGC CGTCCTATCA TCAAGCGTTT TCTGGGGAAA 
CTAACCATCC CAGTCCAGAG GCTGTTGGAG CGACAAGCCA TCGGTGATCA GATGCTCAGC 
TACAACCTTG GCAGAAGGCT CCCAGCTGAC CACGTGAGTG GGTACCTCCA GTTCAGAGTG 
GAGGTCACGT CTTCTGTGCA TGAAGATGCT TCTCCAGAAG CAGTTGGCAC AATACTTGGA 
GTCAATACTG TGAATGGAGA CCTAGGAAGC CCTTCTGATG AGGAGGACAT GCCAGGGGGC 
CACCACGACA GCCCGGTTTG TTCTAATGGA CCAGTTTCTG AGGAAAGTGC TGATGGGACC 
CCCAAGCATT CTTTCAGGAC TAGCTCTACT CTGGAAATAG ACACGGAAGA TTTAACCTCT 
ACTTCTTCAA GGACCTCACC TCCCAGAGGA CGTCAGGATT CCCTCAACGA TTATTTAGAT 
GCTATTGAAC ACAATGGCCA TTCCAGGTCA GGTGCAGCTA CCTGCTCAGA GAGGTCCATG 
GGAGCCTCTC CAAAACTAAG GAGTAGTTTT CCCACCGACA CGAGACTCAA TGCCATGCTT 
CATATTGATT CAGATGAGGA AGACCACGAG TTCCAGCAGG ACCTGGGTTA TCCATCTTCC 
TTGGAGGAGG AAGGAGGGTT GATAATGTTT AGCAGGGCAT CAAGAACTGA TGACGGGAGC 
CTCACATCTC AGACAAAGCC GGAAGACATC CCAGTGGAGA CTGAGGAAGC CTCTATCCAC 
GAACCTGCTT CCTTTGAGGA TAAGCCAGAA CATCTTCCGG AGCTTACAGA GAGCTCCTTA 
CCCTCAGGCC CCATGCCAGA TGAAAGTGAA AATGGCCTGG AGTCTCAACC AGGTGCCGAC 
CAGGGCAGCA CTGAATTGTG TGGCTCTCAG GAGGTCGATC AGCCTACGAG TGGGGCAGAC 
ACGGGCACTT CTGACACGTC GGGAGGAAGC AGAAGAGCCA TCAGTGAGAC AGAGTCCCTT 
GATCAGGGGT CCGAGCCTTC CCAGGTGTCC TCTGAAACAG AACCCAGTGA CCCTGCCAGG 
ACAGAGAGCG TGAGTGAGGC CAGCACCAGG CCTGAGGGAG AGAGTGACCT GGAAGGGGCC 
GACAGCTCCT GCAATGAGAG TGTCACCACG CAGCTGTCCT CGGTGGAGAC ACGGTGCTCA 
TCTCTTGAGA GTGCACGGTT TCCCGAAACC CCAGCCTTTT CTTCTCAGGA GGAGGAAGAT 
GGAGCCTGTG CAGCAGAGCC CACCAGCAGT GGCCCTGCCG AGGGGTCACA GGATTCCATA 
TGCACTCCTG GGTCCTTACC TGTGGTGCAG GTGCCCAGTG GAGAGGAGGA AGGGCCAGGG 
GCAGAATCGG CCACCTTACC TGACCAGGAG GAGGTGGGGG AGGTCTGGCA GCGGAGGGGG 
AGCCTGGAGG GAGCTGCTGC TGCTGCTGCT GCTGCTGAGA GCCAGCCCCA AGAAGAGGGT 
GATGCTGGGG AAGCCCAAGG GGCTTGTGAA GGGGCCACTG CCCAGGAGGA GGGTGCTACT 
GGAGGTTCTC AAGCCAACGG CCACCAGCCA CTGCGATCTC TGCCTTCAGT GCGCCAAGAT 
GTTAGCCGGT ACCAGAGGGT GGACGAGGCT CTCCCACCAA ACTGGGAGGC CCGCATTGAC 
AGCCACGGCA GGATCTTCTA CGTGGATCAC GTGAACAGAA CCACGACGTG GCAGCGGCCC 
ACCGCTCCCC CCGCCCCGCA GGTGCTGCAG AGGTCCAACT CCATACAGCA GATGGAGCAG 
CTGAACCGGC GATACCAGAG TATCCGCAGA ACCATGACTA ACGAGAGGCC CGAGGAAAAT 
AGCAGTGCTC TTGATGGGGC AGGAGAGGAG GCCGACTTTC ACCAAGCCAG TGCAGATTTC 
CGACGGGAGA GCGTCCTGCC CCACTCTACC TCCAGATCCA GGCTCACGTT GCTGTTACAG 
TCGCCCCCTG TGAAGTTCCT CATCAGCCCA GAGTTCTTCA CCGTGCTGCA TTCGAACCCT 
AGTGCCTACC GCATGTTTAC AAACAACACG TGTTTGAAGC ACATGATCAC CAAAGTCCGG 
CGGGACACCC ACCACTTTGA ACGCTACCAG CATAACCGGG ACCTTGTGGG ATTCCTCAAC 
ATGTTTGCAA ACAAACAACT GGAGCTGCCG AGGGGCTGGG AAATGAAACA CGATCATCAG 
GGCAAGGCAT TTTTTGTTGA CCACAACTCC CGGACCACCA CTTTCATTGA CCCCCGGCTC 
CCACTCCAGA GCAGCAGACC CACGAGTGCG CTGGTGCATC GACAGCATCT GACGAGGCAG 
CGCAGCCACA GTGCAGGCGA GGTGGGAGAA GATTCTCGAC ATGCCGGACC ACCAGTTCTT 
CCCAGGCCGT CGAGTACGTT CAACACAGTC AGCAGGCCGC AGTACCAGGA CATGGTTCCA 
GTGGCTTACA ATGACAAGAT TGTTGCATTT CTTCGCCAAC CCAACATCTT TGAAATTCTA 
CAGGAGCGTC AACCTGATCT TACCAGGAAT CACTCACTCA GGGAGAAGAT CCAATTTATC 
CGAACTGAAG GGACCCCAGG ATTGGTGCGT CTTTCAAGTG ATGCAGACCT TGTTATGTTG 
CTAAGTTTAT TTGAAGAAGA GATAATGTCA TACGTGCCTC CACATGCCTT ACTCCATCCC 
AGCTACTGTC AGTCCCCACG TGGCTCCCCT GTGTCCTCTC CCCAGAACTC GCCAGGTACT 
CAGCGTGCCA ACGCCCGGGC TCCCGCCCCT TACAAGCGAG ACTTTGAAGC CAAACTGAGG 
AATTTTTACA GGAAGTTAGA GACTAAAGGA TATGGACAAG GCCCAGGGAA ATTAAAGTTA 
ATTATCAGAA GAGATCACTT GCTCGAAGAT GCTTTTAATC AGATTATGGG CTATTCCAGA 
AAAGACCTGC AGAGAAATAA GCTGTACGTC ACATTTGTCG GGGAGGAAGG GCTGGATTAC 
AGTGGACCTT CCAGAGAGTT TTTCTTCCTG GTATCCAGAG AGCTCTTTAA CCCATATTAC 
GGCTTATTTG AATATTCAGC CAATGACACA TACACAGTAC AAATAAGTCC CATGTCTGCT 
TTTGTAGACA ATCACCATGA ATGGTTCCGG TTCAGTGGTA GGATCCTTGG TCTTGCGCTG 
ATTCACCAGT ATTTGTTGGA TGCCTTCTTC ACGCGGCCCT TCTATAAGGC TCTTCTCAGA 
ATTCTGTGTG ACCTGAGTGA TCTAGAATAC CTTGATGAGG AGTTCCACCA GAGCCTGCAG 
TGGATGAAAG ACAATGATAT CCATGACATT CTGGACCTCA CGTTCACAGT GAACGAAGAA 
GTTTTCGGGC AGATAACTGA ACGAGAATTA AAACCAGGGG GTGCCAACAT CCCAGTCACA 
GAGAAGAACA AGAAGGAGTA CATCGAGAGG ATGGTGAAAT GGAGGATTGA GCGGGGTGTT 
GTGCAGCAAA CGGAGAGCTT AGTGCGTGGC TTCTACGAGG TGGTGGATGC CAGGCTAGTA 
TCTGTTTTTG ATGCAAGAGA ACTGGAACTG GTCATCGCAG GCACAGCTGA AATAGACCTA 
AGTGATTGGA GAAACAACAC AGAATACAGA GGAGGTTATC ATGACAATCA TATTGTAATT 
CGGTGGTTCT GGGCTGCAGT GGAGAGATTC AACAATGAAC AACGATTAAG GTTGTTACAG 
TTTGTCACAG GCACATCCAG CATTCCCTAT GAAGGATTTG CTTCTCTCCG AGGGAGTAAT 
GGCCCAAGAA GATTCTGCGT GGAGAAATGG GGGAAAATCA CCGCTCTTCC CAGAGCTCAC 
ACTTGTTTTA ACCGTCTGGA TCTCCCTCCA TACCCATCCT TCTCCATGCT TTATGAAAAA 
CTTTTGACAG CAGTTGAGGA GACCAGTACC TTTGGACTTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020930201.1
CDS	139..4863
Translation	MASSAREHLLFVRRRNPQMRYTLSPENLQSLAAQSSMPENMTLQRANSDTDLVTSESRSSLTASMYEYTLGQAQNLIIFWDIKEEVDPSDWIGLYHIDENSPANFWDSKNRGVTGTQKGQIVWRIEPGPYFMEPEIKICFKYYHGISGALRATTPCITVKNPAVMMGAEGMEGGAAGSQHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTCAHHGQERRSTIISNTTNPIWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAIGDQMLSYNLGRRLPADHVSGYLQFRVEVTSSVHEDASPEAVGTILGVNTVNGDLGSPSDEEDMPGGHHDSPVCSNGPVSEESADGTPKHSFRTSSTLEIDTEDLTSTSSRTSPPRGRQDSLNDYLDAIEHNGHSRSGAATCSERSMGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMFSRASRTDDGSLTSQTKPEDIPVETEEASIHEPASFEDKPEHLPELTESSLPSGPMPDESENGLESQPGADQGSTELCGSQEVDQPTSGADTGTSDTSGGSRRAISETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDLEGADSSCNESVTTQLSSVETRCSSLESARFPETPAFSSQEEEDGACAAEPTSSGPAEGSQDSICTPGSLPVVQVPSGEEEGPGAESATLPDQEEVGEVWQRRGSLEGAAAAAAAAESQPQEEGDAGEAQGACEGATAQEEGATGGSQANGHQPLRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENSSALDGAGEEADFHQASADFRRESVLPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNIFEILQERQPDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_021074542.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021074542.1

ORF Insert Sequence:

ATGGCTAGCT CAGCCCGGGA GCACCTGCTT TTTGTGCGGC GGCGAAATCC CCAGATGCGG 
TACACTCTGA GTCCAGAGAA CCTCCAGAGC CTGGCTGCCC AGAGCTCCAT GCCTGAGAAC 
ATGACCCTGC AGCGGGCCAA CAGCGACACA GATCTGGTGA CTTCTGAGAG CCGGTCCAGC 
CTGACTGCCA GCATGTATGA GTACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAAG AGGAGGTGGA CCCGAGTGAT TGGATTGGAC TTTATCACAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAAAAC AGGGGTGTGA CTGGAACACA AAAAGGACAA 
ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAGC CGGAGATAAA AATCTGTTTC 
AAATACTACC ACGGCATTAG TGGAGCCCTG CGAGCCACGA CCCCCTGCAT CACGGTGAAG 
AACCCTGCTG TGATGATGGG GGCAGAAGGC ATGGAAGGAG GTGCTGCAGG CAGTCAGCAT 
TCTCGGAAAC TCGTTAGCTT TACGTTGTCA GATCTTAGGG CAGTTGGGCT AAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTGAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT 
TTCCCCACCT GTGCCCACCA TGGGCAGGAG AGGAGGTCTA CCATCATCAG TAACACCACT 
AATCCAATCT GGCACCGAGA GAAATACTCC TTTTTTGCAC TCCTTACTGA TGTCTTAGAA 
ATTGAAATTA AAGACAAATT TGCCAAGAGC CGTCCTATCA TCAAGCGTTT TCTGGGGAAA 
CTAACCATCC CAGTCCAGAG GCTGTTGGAG CGACAAGCCA TCGGTGATCA GATGCTCAGC 
TACAACCTTG GCAGAAGGCT CCCAGCTGAC CACGTGAGTG GGTACCTCCA GTTCAGAGTG 
GAGGTCACGT CTTCTGTGCA TGAAGATGCT TCTCCAGAAG CAGTTGGCAC AATACTTGGA 
GTCAATACTG TGAATGGAGA CCTAGGAAGC CCTTCTGATG AGGAGGACAT GCCAGGGGGC 
CACCACGACA GCCCGGTTTG TTCTAATGGA CCAGTTTCTG AGGAAAGTGC TGATGGGACC 
CCCAAGCATT CTTTCAGGAC TAGCTCTACT CTGGAAATAG ACACGGAAGA TTTAACCTCT 
ACTTCTTCAA GGACCTCACC TCCCAGAGGA CGTCAGGATT CCCTCAACGA TTATTTAGAT 
GCTATTGAAC ACAATGGCCA TTCCAGGTCA GGTGCAGCTA CCTGCTCAGA GAGGTCCATG 
GGAGCCTCTC CAAAACTAAG GAGTAGTTTT CCCACCGACA CGAGACTCAA TGCCATGCTT 
CATATTGATT CAGATGAGGA AGACCACGAG TTCCAGCAGG ACCTGGGTTA TCCATCTTCC 
TTGGAGGAGG AAGGAGGGTT GATAATGTTT AGCAGGGCAT CAAGAACTGA TGACGGGAGC 
CTCACATCTC AGACAAAGCC GGAAGACATC CCAGTGGAGA CTGAGGAAGC CTCTATCCAC 
GAACCTGCTT CCTTTGAGGA TAAGCCAGAA CATCTTCCGG AGCTTACAGA GAGCTCCTTA 
CCCTCAGGCC CCATGCCAGA TGAAAGTGAA AATGGCCTGG AGTCTCAACC AGGTGCCGAC 
CAGGGCAGCA CTGAATTGTG TGGCTCTCAG GAGGTCGATC AGCCTACGAG TGGGGCAGAC 
ACGGGCACTT CTGACACGTC GGGAGGAAGC AGAAGAGCCA TCAGTGAGAC AGAGTCCCTT 
GATCAGGGGT CCGAGCCTTC CCAGGTGTCC TCTGAAACAG AACCCAGTGA CCCTGCCAGG 
ACAGAGAGCG TGAGTGAGGC CAGCACCAGG CCTGAGGGAG AGAGTGACCT GGAAGGGGCC 
GACAGCTCCT GCAATGAGAG TGTCACCACG CAGCTGTCCT CGGTGGAGAC ACGGTGCTCA 
TCTCTTGAGA GTGCACGGTT TCCCGAAACC CCAGCCTTTT CTTCTCAGGA GGAGGAAGAT 
GGAGCCTGTG CAGCAGAGCC CACCAGCAGT GGCCCTGCCG AGGGGTCACA GGATTCCATA 
TGCACTCCTG GGTCCTTACC TGTGGTGCAG GTGCCCAGTG GAGAGGAGGA AGGGCCAGGG 
GCAGAATCGG CCACCTTACC TGACCAGGAG GAGGTGGGGG AGGTCTGGCA GCGGAGGGGG 
AGCCTGGAGG GAGCTGCTGC TGCTGCTGCT GCTGCTGAGA GCCAGCCCCA AGAAGAGGGT 
GATGCTGGGG AAGCCCAAGG GGCTTGTGAA GGGGCCACTG CCCAGGAGGA GGGTGCTACT 
GGAGGTTCTC AAGCCAACGG CCACCAGCCA CTGCGATCTC TGCCTTCAGT GCGCCAAGAT 
GTTAGCCGGT ACCAGAGGGT GGACGAGGCT CTCCCACCAA ACTGGGAGGC CCGCATTGAC 
AGCCACGGCA GGATCTTCTA CGTGGATCAC GTGAACAGAA CCACGACGTG GCAGCGGCCC 
ACCGCTCCCC CCGCCCCGCA GGTGCTGCAG AGGTCCAACT CCATACAGCA GATGGAGCAG 
CTGAACCGGC GATACCAGAG TATCCGCAGA ACCATGACTA ACGAGAGGCC CGAGGAAAAT 
AGCAGTGCTC TTGATGGGGC AGGAGAGGAG GCCGACTTTC ACCAAGCCAG TGCAGATTTC 
CGACGGGAGA GCGTCCTGCC CCACTCTACC TCCAGATCCA GGCTCACGTT GCTGTTACAG 
TCGCCCCCTG TGAAGTTCCT CATCAGCCCA GAGTTCTTCA CCGTGCTGCA TTCGAACCCT 
AGTGCCTACC GCATGTTTAC AAACAACACG TGTTTGAAGC ACATGATCAC CAAAGTCCGG 
CGGGACACCC ACCACTTTGA ACGCTACCAG CATAACCGGG ACCTTGTGGG ATTCCTCAAC 
ATGTTTGCAA ACAAACAACT GGAGCTGCCG AGGGGCTGGG AAATGAAACA CGATCATCAG 
GGCAAGGCAT TTTTTGTTGA CCACAACTCC CGGACCACCA CTTTCATTGA CCCCCGGCTC 
CCACTCCAGA GCAGCAGACC CACGAGTGCG CTGGTGCATC GACAGCATCT GACGAGGCAG 
CGCAGCCACA GTGCAGGCGA GGTGGGAGAA GATTCTCGAC ATGCCGGACC ACCAGTTCTT 
CCCAGGCCGT CGAGTACGTT CAACACAGTC AGCAGGCCGC AGTACCAGGA CATGGTTCCA 
GTGGCTTACA ATGACAAGAT TGTTGCATTT CTTCGCCAAC CCAACATCTT TGAAATTCTA 
CAGGAGCGTC AACCTGATCT TACCAGGAAT CACTCACTCA GGGAGAAGAT CCAATTTATC 
CGAACTGAAG GGACCCCAGG ATTGGTGCGT CTTTCAAGTG ATGCAGACCT TGTTATGTTG 
CTAAGTTTAT TTGAAGAAGA GATAATGTCA TACGTGCCTC CACATGCCTT ACTCCATCCC 
AGCTACTGTC AGTCCCCACG TGGCTCCCCT GTGTCCTCTC CCCAGAACTC GCCAGGTACT 
CAGCGTGCCA ACGCCCGGGC TCCCGCCCCT TACAAGCGAG ACTTTGAAGC CAAACTGAGG 
AATTTTTACA GGAAGTTAGA GACTAAAGGA TATGGACAAG GCCCAGGGAA ATTAAAGTTA 
ATTATCAGAA GAGATCACTT GCTCGAAGAT GCTTTTAATC AGATTATGGG CTATTCCAGA 
AAAGACCTGC AGAGAAATAA GCTGTACGTC ACATTTGTCG GGGAGGAAGG GCTGGATTAC 
AGTGGACCTT CCAGAGAGTT TTTCTTCCTG GTATCCAGAG AGCTCTTTAA CCCATATTAC 
GGCTTATTTG AATATTCAGC CAATGACACA TACACAGTAC AAATAAGTCC CATGTCTGCT 
TTTGTAGACA ATCACCATGA ATGGTTCCGG TTCAGTGGTA GGATCCTTGG TCTTGCGCTG 
ATTCACCAGT ATTTGTTGGA TGCCTTCTTC ACGCGGCCCT TCTATAAGGC TCTTCTCAGA 
ATTCTGTGTG ACCTGAGTGA TCTAGAATAC CTTGATGAGG AGTTCCACCA GAGCCTGCAG 
TGGATGAAAG ACAATGATAT CCATGACATT CTGGACCTCA CGTTCACAGT GAACGAAGAA 
GTTTTCGGGC AGATAACTGA ACGAGAATTA AAACCAGGGG GTGCCAACAT CCCAGTCACA 
GAGAAGAACA AGAAGGAGTA CATCGAGAGG ATGGTGAAAT GGAGGATTGA GCGGGGTGTT 
GTGCAGCAAA CGGAGAGCTT AGTGCGTGGC TTCTACGAGG TGGTGGATGC CAGGCTAGTA 
TCTGTTTTTG ATGCAAGAGA ACTGGAACTG GTCATCGCAG GCACAGCTGA AATAGACCTA 
AGTGATTGGA GAAACAACAC AGAATACAGA GGAGGTTATC ATGACAATCA TATTGTAATT 
CGGTGGTTCT GGGCTGCAGT GGAGAGATTC AACAATGAAC AACGATTAAG GTTGTTACAG 
TTTGTCACAG GCACATCCAG CATTCCCTAT GAAGGATTTG CTTCTCTCCG AGGGAGTAAT 
GGCCCAAGAA GATTCTGCGT GGAGAAATGG GGGAAAATCA CCGCTCTTCC CAGAGCTCAC 
ACTTGTTTTA ACCGTCTGGA TCTCCCTCCA TACCCATCCT TCTCCATGCT TTATGAAAAA 
CTTTTGACAG CAGTTGAGGA GACCAGTACC TTTGGACTTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW2 ( XM_021074545.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020930204.1 Sus scrofa HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OSe01255
Clone ID Related Accession (Same CDS sequence)	XM_021074541.1 , XM_021074543.1 , NM_001198924.1 , XM_021074542.1 , XM_021074545.1 , XM_021074544.1
Accession Version	XM_021074545.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Sus scrofa(pig)
Product	E3 ubiquitin-protein ligase HECW2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010457.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAGCT CAGCCCGGGA GCACCTGCTT TTTGTGCGGC GGCGAAATCC CCAGATGCGG 
TACACTCTGA GTCCAGAGAA CCTCCAGAGC CTGGCTGCCC AGAGCTCCAT GCCTGAGAAC 
ATGACCCTGC AGCGGGCCAA CAGCGACACA GATCTGGTGA CTTCTGAGAG CCGGTCCAGC 
CTGACTGCCA GCATGTATGA GTACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAAG AGGAGGTGGA CCCGAGTGAT TGGATTGGAC TTTATCACAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAAAAC AGGGGTGTGA CTGGAACACA AAAAGGACAA 
ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAGC CGGAGATAAA AATCTGTTTC 
AAATACTACC ACGGCATTAG TGGAGCCCTG CGAGCCACGA CCCCCTGCAT CACGGTGAAG 
AACCCTGCTG TGATGATGGG GGCAGAAGGC ATGGAAGGAG GTGCTGCAGG CAGTCAGCAT 
TCTCGGAAAC TCGTTAGCTT TACGTTGTCA GATCTTAGGG CAGTTGGGCT AAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTGAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT 
TTCCCCACCT GTGCCCACCA TGGGCAGGAG AGGAGGTCTA CCATCATCAG TAACACCACT 
AATCCAATCT GGCACCGAGA GAAATACTCC TTTTTTGCAC TCCTTACTGA TGTCTTAGAA 
ATTGAAATTA AAGACAAATT TGCCAAGAGC CGTCCTATCA TCAAGCGTTT TCTGGGGAAA 
CTAACCATCC CAGTCCAGAG GCTGTTGGAG CGACAAGCCA TCGGTGATCA GATGCTCAGC 
TACAACCTTG GCAGAAGGCT CCCAGCTGAC CACGTGAGTG GGTACCTCCA GTTCAGAGTG 
GAGGTCACGT CTTCTGTGCA TGAAGATGCT TCTCCAGAAG CAGTTGGCAC AATACTTGGA 
GTCAATACTG TGAATGGAGA CCTAGGAAGC CCTTCTGATG AGGAGGACAT GCCAGGGGGC 
CACCACGACA GCCCGGTTTG TTCTAATGGA CCAGTTTCTG AGGAAAGTGC TGATGGGACC 
CCCAAGCATT CTTTCAGGAC TAGCTCTACT CTGGAAATAG ACACGGAAGA TTTAACCTCT 
ACTTCTTCAA GGACCTCACC TCCCAGAGGA CGTCAGGATT CCCTCAACGA TTATTTAGAT 
GCTATTGAAC ACAATGGCCA TTCCAGGTCA GGTGCAGCTA CCTGCTCAGA GAGGTCCATG 
GGAGCCTCTC CAAAACTAAG GAGTAGTTTT CCCACCGACA CGAGACTCAA TGCCATGCTT 
CATATTGATT CAGATGAGGA AGACCACGAG TTCCAGCAGG ACCTGGGTTA TCCATCTTCC 
TTGGAGGAGG AAGGAGGGTT GATAATGTTT AGCAGGGCAT CAAGAACTGA TGACGGGAGC 
CTCACATCTC AGACAAAGCC GGAAGACATC CCAGTGGAGA CTGAGGAAGC CTCTATCCAC 
GAACCTGCTT CCTTTGAGGA TAAGCCAGAA CATCTTCCGG AGCTTACAGA GAGCTCCTTA 
CCCTCAGGCC CCATGCCAGA TGAAAGTGAA AATGGCCTGG AGTCTCAACC AGGTGCCGAC 
CAGGGCAGCA CTGAATTGTG TGGCTCTCAG GAGGTCGATC AGCCTACGAG TGGGGCAGAC 
ACGGGCACTT CTGACACGTC GGGAGGAAGC AGAAGAGCCA TCAGTGAGAC AGAGTCCCTT 
GATCAGGGGT CCGAGCCTTC CCAGGTGTCC TCTGAAACAG AACCCAGTGA CCCTGCCAGG 
ACAGAGAGCG TGAGTGAGGC CAGCACCAGG CCTGAGGGAG AGAGTGACCT GGAAGGGGCC 
GACAGCTCCT GCAATGAGAG TGTCACCACG CAGCTGTCCT CGGTGGAGAC ACGGTGCTCA 
TCTCTTGAGA GTGCACGGTT TCCCGAAACC CCAGCCTTTT CTTCTCAGGA GGAGGAAGAT 
GGAGCCTGTG CAGCAGAGCC CACCAGCAGT GGCCCTGCCG AGGGGTCACA GGATTCCATA 
TGCACTCCTG GGTCCTTACC TGTGGTGCAG GTGCCCAGTG GAGAGGAGGA AGGGCCAGGG 
GCAGAATCGG CCACCTTACC TGACCAGGAG GAGGTGGGGG AGGTCTGGCA GCGGAGGGGG 
AGCCTGGAGG GAGCTGCTGC TGCTGCTGCT GCTGCTGAGA GCCAGCCCCA AGAAGAGGGT 
GATGCTGGGG AAGCCCAAGG GGCTTGTGAA GGGGCCACTG CCCAGGAGGA GGGTGCTACT 
GGAGGTTCTC AAGCCAACGG CCACCAGCCA CTGCGATCTC TGCCTTCAGT GCGCCAAGAT 
GTTAGCCGGT ACCAGAGGGT GGACGAGGCT CTCCCACCAA ACTGGGAGGC CCGCATTGAC 
AGCCACGGCA GGATCTTCTA CGTGGATCAC GTGAACAGAA CCACGACGTG GCAGCGGCCC 
ACCGCTCCCC CCGCCCCGCA GGTGCTGCAG AGGTCCAACT CCATACAGCA GATGGAGCAG 
CTGAACCGGC GATACCAGAG TATCCGCAGA ACCATGACTA ACGAGAGGCC CGAGGAAAAT 
AGCAGTGCTC TTGATGGGGC AGGAGAGGAG GCCGACTTTC ACCAAGCCAG TGCAGATTTC 
CGACGGGAGA GCGTCCTGCC CCACTCTACC TCCAGATCCA GGCTCACGTT GCTGTTACAG 
TCGCCCCCTG TGAAGTTCCT CATCAGCCCA GAGTTCTTCA CCGTGCTGCA TTCGAACCCT 
AGTGCCTACC GCATGTTTAC AAACAACACG TGTTTGAAGC ACATGATCAC CAAAGTCCGG 
CGGGACACCC ACCACTTTGA ACGCTACCAG CATAACCGGG ACCTTGTGGG ATTCCTCAAC 
ATGTTTGCAA ACAAACAACT GGAGCTGCCG AGGGGCTGGG AAATGAAACA CGATCATCAG 
GGCAAGGCAT TTTTTGTTGA CCACAACTCC CGGACCACCA CTTTCATTGA CCCCCGGCTC 
CCACTCCAGA GCAGCAGACC CACGAGTGCG CTGGTGCATC GACAGCATCT GACGAGGCAG 
CGCAGCCACA GTGCAGGCGA GGTGGGAGAA GATTCTCGAC ATGCCGGACC ACCAGTTCTT 
CCCAGGCCGT CGAGTACGTT CAACACAGTC AGCAGGCCGC AGTACCAGGA CATGGTTCCA 
GTGGCTTACA ATGACAAGAT TGTTGCATTT CTTCGCCAAC CCAACATCTT TGAAATTCTA 
CAGGAGCGTC AACCTGATCT TACCAGGAAT CACTCACTCA GGGAGAAGAT CCAATTTATC 
CGAACTGAAG GGACCCCAGG ATTGGTGCGT CTTTCAAGTG ATGCAGACCT TGTTATGTTG 
CTAAGTTTAT TTGAAGAAGA GATAATGTCA TACGTGCCTC CACATGCCTT ACTCCATCCC 
AGCTACTGTC AGTCCCCACG TGGCTCCCCT GTGTCCTCTC CCCAGAACTC GCCAGGTACT 
CAGCGTGCCA ACGCCCGGGC TCCCGCCCCT TACAAGCGAG ACTTTGAAGC CAAACTGAGG 
AATTTTTACA GGAAGTTAGA GACTAAAGGA TATGGACAAG GCCCAGGGAA ATTAAAGTTA 
ATTATCAGAA GAGATCACTT GCTCGAAGAT GCTTTTAATC AGATTATGGG CTATTCCAGA 
AAAGACCTGC AGAGAAATAA GCTGTACGTC ACATTTGTCG GGGAGGAAGG GCTGGATTAC 
AGTGGACCTT CCAGAGAGTT TTTCTTCCTG GTATCCAGAG AGCTCTTTAA CCCATATTAC 
GGCTTATTTG AATATTCAGC CAATGACACA TACACAGTAC AAATAAGTCC CATGTCTGCT 
TTTGTAGACA ATCACCATGA ATGGTTCCGG TTCAGTGGTA GGATCCTTGG TCTTGCGCTG 
ATTCACCAGT ATTTGTTGGA TGCCTTCTTC ACGCGGCCCT TCTATAAGGC TCTTCTCAGA 
ATTCTGTGTG ACCTGAGTGA TCTAGAATAC CTTGATGAGG AGTTCCACCA GAGCCTGCAG 
TGGATGAAAG ACAATGATAT CCATGACATT CTGGACCTCA CGTTCACAGT GAACGAAGAA 
GTTTTCGGGC AGATAACTGA ACGAGAATTA AAACCAGGGG GTGCCAACAT CCCAGTCACA 
GAGAAGAACA AGAAGGAGTA CATCGAGAGG ATGGTGAAAT GGAGGATTGA GCGGGGTGTT 
GTGCAGCAAA CGGAGAGCTT AGTGCGTGGC TTCTACGAGG TGGTGGATGC CAGGCTAGTA 
TCTGTTTTTG ATGCAAGAGA ACTGGAACTG GTCATCGCAG GCACAGCTGA AATAGACCTA 
AGTGATTGGA GAAACAACAC AGAATACAGA GGAGGTTATC ATGACAATCA TATTGTAATT 
CGGTGGTTCT GGGCTGCAGT GGAGAGATTC AACAATGAAC AACGATTAAG GTTGTTACAG 
TTTGTCACAG GCACATCCAG CATTCCCTAT GAAGGATTTG CTTCTCTCCG AGGGAGTAAT 
GGCCCAAGAA GATTCTGCGT GGAGAAATGG GGGAAAATCA CCGCTCTTCC CAGAGCTCAC 
ACTTGTTTTA ACCGTCTGGA TCTCCCTCCA TACCCATCCT TCTCCATGCT TTATGAAAAA 
CTTTTGACAG CAGTTGAGGA GACCAGTACC TTTGGACTTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020930204.1
CDS	116..4840
Translation	MASSAREHLLFVRRRNPQMRYTLSPENLQSLAAQSSMPENMTLQRANSDTDLVTSESRSSLTASMYEYTLGQAQNLIIFWDIKEEVDPSDWIGLYHIDENSPANFWDSKNRGVTGTQKGQIVWRIEPGPYFMEPEIKICFKYYHGISGALRATTPCITVKNPAVMMGAEGMEGGAAGSQHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTCAHHGQERRSTIISNTTNPIWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAIGDQMLSYNLGRRLPADHVSGYLQFRVEVTSSVHEDASPEAVGTILGVNTVNGDLGSPSDEEDMPGGHHDSPVCSNGPVSEESADGTPKHSFRTSSTLEIDTEDLTSTSSRTSPPRGRQDSLNDYLDAIEHNGHSRSGAATCSERSMGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMFSRASRTDDGSLTSQTKPEDIPVETEEASIHEPASFEDKPEHLPELTESSLPSGPMPDESENGLESQPGADQGSTELCGSQEVDQPTSGADTGTSDTSGGSRRAISETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDLEGADSSCNESVTTQLSSVETRCSSLESARFPETPAFSSQEEEDGACAAEPTSSGPAEGSQDSICTPGSLPVVQVPSGEEEGPGAESATLPDQEEVGEVWQRRGSLEGAAAAAAAAESQPQEEGDAGEAQGACEGATAQEEGATGGSQANGHQPLRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENSSALDGAGEEADFHQASADFRRESVLPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNIFEILQERQPDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_021074545.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021074545.1

ORF Insert Sequence:

ATGGCTAGCT CAGCCCGGGA GCACCTGCTT TTTGTGCGGC GGCGAAATCC CCAGATGCGG 
TACACTCTGA GTCCAGAGAA CCTCCAGAGC CTGGCTGCCC AGAGCTCCAT GCCTGAGAAC 
ATGACCCTGC AGCGGGCCAA CAGCGACACA GATCTGGTGA CTTCTGAGAG CCGGTCCAGC 
CTGACTGCCA GCATGTATGA GTACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAAG AGGAGGTGGA CCCGAGTGAT TGGATTGGAC TTTATCACAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAAAAC AGGGGTGTGA CTGGAACACA AAAAGGACAA 
ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAGC CGGAGATAAA AATCTGTTTC 
AAATACTACC ACGGCATTAG TGGAGCCCTG CGAGCCACGA CCCCCTGCAT CACGGTGAAG 
AACCCTGCTG TGATGATGGG GGCAGAAGGC ATGGAAGGAG GTGCTGCAGG CAGTCAGCAT 
TCTCGGAAAC TCGTTAGCTT TACGTTGTCA GATCTTAGGG CAGTTGGGCT AAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTGAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT 
TTCCCCACCT GTGCCCACCA TGGGCAGGAG AGGAGGTCTA CCATCATCAG TAACACCACT 
AATCCAATCT GGCACCGAGA GAAATACTCC TTTTTTGCAC TCCTTACTGA TGTCTTAGAA 
ATTGAAATTA AAGACAAATT TGCCAAGAGC CGTCCTATCA TCAAGCGTTT TCTGGGGAAA 
CTAACCATCC CAGTCCAGAG GCTGTTGGAG CGACAAGCCA TCGGTGATCA GATGCTCAGC 
TACAACCTTG GCAGAAGGCT CCCAGCTGAC CACGTGAGTG GGTACCTCCA GTTCAGAGTG 
GAGGTCACGT CTTCTGTGCA TGAAGATGCT TCTCCAGAAG CAGTTGGCAC AATACTTGGA 
GTCAATACTG TGAATGGAGA CCTAGGAAGC CCTTCTGATG AGGAGGACAT GCCAGGGGGC 
CACCACGACA GCCCGGTTTG TTCTAATGGA CCAGTTTCTG AGGAAAGTGC TGATGGGACC 
CCCAAGCATT CTTTCAGGAC TAGCTCTACT CTGGAAATAG ACACGGAAGA TTTAACCTCT 
ACTTCTTCAA GGACCTCACC TCCCAGAGGA CGTCAGGATT CCCTCAACGA TTATTTAGAT 
GCTATTGAAC ACAATGGCCA TTCCAGGTCA GGTGCAGCTA CCTGCTCAGA GAGGTCCATG 
GGAGCCTCTC CAAAACTAAG GAGTAGTTTT CCCACCGACA CGAGACTCAA TGCCATGCTT 
CATATTGATT CAGATGAGGA AGACCACGAG TTCCAGCAGG ACCTGGGTTA TCCATCTTCC 
TTGGAGGAGG AAGGAGGGTT GATAATGTTT AGCAGGGCAT CAAGAACTGA TGACGGGAGC 
CTCACATCTC AGACAAAGCC GGAAGACATC CCAGTGGAGA CTGAGGAAGC CTCTATCCAC 
GAACCTGCTT CCTTTGAGGA TAAGCCAGAA CATCTTCCGG AGCTTACAGA GAGCTCCTTA 
CCCTCAGGCC CCATGCCAGA TGAAAGTGAA AATGGCCTGG AGTCTCAACC AGGTGCCGAC 
CAGGGCAGCA CTGAATTGTG TGGCTCTCAG GAGGTCGATC AGCCTACGAG TGGGGCAGAC 
ACGGGCACTT CTGACACGTC GGGAGGAAGC AGAAGAGCCA TCAGTGAGAC AGAGTCCCTT 
GATCAGGGGT CCGAGCCTTC CCAGGTGTCC TCTGAAACAG AACCCAGTGA CCCTGCCAGG 
ACAGAGAGCG TGAGTGAGGC CAGCACCAGG CCTGAGGGAG AGAGTGACCT GGAAGGGGCC 
GACAGCTCCT GCAATGAGAG TGTCACCACG CAGCTGTCCT CGGTGGAGAC ACGGTGCTCA 
TCTCTTGAGA GTGCACGGTT TCCCGAAACC CCAGCCTTTT CTTCTCAGGA GGAGGAAGAT 
GGAGCCTGTG CAGCAGAGCC CACCAGCAGT GGCCCTGCCG AGGGGTCACA GGATTCCATA 
TGCACTCCTG GGTCCTTACC TGTGGTGCAG GTGCCCAGTG GAGAGGAGGA AGGGCCAGGG 
GCAGAATCGG CCACCTTACC TGACCAGGAG GAGGTGGGGG AGGTCTGGCA GCGGAGGGGG 
AGCCTGGAGG GAGCTGCTGC TGCTGCTGCT GCTGCTGAGA GCCAGCCCCA AGAAGAGGGT 
GATGCTGGGG AAGCCCAAGG GGCTTGTGAA GGGGCCACTG CCCAGGAGGA GGGTGCTACT 
GGAGGTTCTC AAGCCAACGG CCACCAGCCA CTGCGATCTC TGCCTTCAGT GCGCCAAGAT 
GTTAGCCGGT ACCAGAGGGT GGACGAGGCT CTCCCACCAA ACTGGGAGGC CCGCATTGAC 
AGCCACGGCA GGATCTTCTA CGTGGATCAC GTGAACAGAA CCACGACGTG GCAGCGGCCC 
ACCGCTCCCC CCGCCCCGCA GGTGCTGCAG AGGTCCAACT CCATACAGCA GATGGAGCAG 
CTGAACCGGC GATACCAGAG TATCCGCAGA ACCATGACTA ACGAGAGGCC CGAGGAAAAT 
AGCAGTGCTC TTGATGGGGC AGGAGAGGAG GCCGACTTTC ACCAAGCCAG TGCAGATTTC 
CGACGGGAGA GCGTCCTGCC CCACTCTACC TCCAGATCCA GGCTCACGTT GCTGTTACAG 
TCGCCCCCTG TGAAGTTCCT CATCAGCCCA GAGTTCTTCA CCGTGCTGCA TTCGAACCCT 
AGTGCCTACC GCATGTTTAC AAACAACACG TGTTTGAAGC ACATGATCAC CAAAGTCCGG 
CGGGACACCC ACCACTTTGA ACGCTACCAG CATAACCGGG ACCTTGTGGG ATTCCTCAAC 
ATGTTTGCAA ACAAACAACT GGAGCTGCCG AGGGGCTGGG AAATGAAACA CGATCATCAG 
GGCAAGGCAT TTTTTGTTGA CCACAACTCC CGGACCACCA CTTTCATTGA CCCCCGGCTC 
CCACTCCAGA GCAGCAGACC CACGAGTGCG CTGGTGCATC GACAGCATCT GACGAGGCAG 
CGCAGCCACA GTGCAGGCGA GGTGGGAGAA GATTCTCGAC ATGCCGGACC ACCAGTTCTT 
CCCAGGCCGT CGAGTACGTT CAACACAGTC AGCAGGCCGC AGTACCAGGA CATGGTTCCA 
GTGGCTTACA ATGACAAGAT TGTTGCATTT CTTCGCCAAC CCAACATCTT TGAAATTCTA 
CAGGAGCGTC AACCTGATCT TACCAGGAAT CACTCACTCA GGGAGAAGAT CCAATTTATC 
CGAACTGAAG GGACCCCAGG ATTGGTGCGT CTTTCAAGTG ATGCAGACCT TGTTATGTTG 
CTAAGTTTAT TTGAAGAAGA GATAATGTCA TACGTGCCTC CACATGCCTT ACTCCATCCC 
AGCTACTGTC AGTCCCCACG TGGCTCCCCT GTGTCCTCTC CCCAGAACTC GCCAGGTACT 
CAGCGTGCCA ACGCCCGGGC TCCCGCCCCT TACAAGCGAG ACTTTGAAGC CAAACTGAGG 
AATTTTTACA GGAAGTTAGA GACTAAAGGA TATGGACAAG GCCCAGGGAA ATTAAAGTTA 
ATTATCAGAA GAGATCACTT GCTCGAAGAT GCTTTTAATC AGATTATGGG CTATTCCAGA 
AAAGACCTGC AGAGAAATAA GCTGTACGTC ACATTTGTCG GGGAGGAAGG GCTGGATTAC 
AGTGGACCTT CCAGAGAGTT TTTCTTCCTG GTATCCAGAG AGCTCTTTAA CCCATATTAC 
GGCTTATTTG AATATTCAGC CAATGACACA TACACAGTAC AAATAAGTCC CATGTCTGCT 
TTTGTAGACA ATCACCATGA ATGGTTCCGG TTCAGTGGTA GGATCCTTGG TCTTGCGCTG 
ATTCACCAGT ATTTGTTGGA TGCCTTCTTC ACGCGGCCCT TCTATAAGGC TCTTCTCAGA 
ATTCTGTGTG ACCTGAGTGA TCTAGAATAC CTTGATGAGG AGTTCCACCA GAGCCTGCAG 
TGGATGAAAG ACAATGATAT CCATGACATT CTGGACCTCA CGTTCACAGT GAACGAAGAA 
GTTTTCGGGC AGATAACTGA ACGAGAATTA AAACCAGGGG GTGCCAACAT CCCAGTCACA 
GAGAAGAACA AGAAGGAGTA CATCGAGAGG ATGGTGAAAT GGAGGATTGA GCGGGGTGTT 
GTGCAGCAAA CGGAGAGCTT AGTGCGTGGC TTCTACGAGG TGGTGGATGC CAGGCTAGTA 
TCTGTTTTTG ATGCAAGAGA ACTGGAACTG GTCATCGCAG GCACAGCTGA AATAGACCTA 
AGTGATTGGA GAAACAACAC AGAATACAGA GGAGGTTATC ATGACAATCA TATTGTAATT 
CGGTGGTTCT GGGCTGCAGT GGAGAGATTC AACAATGAAC AACGATTAAG GTTGTTACAG 
TTTGTCACAG GCACATCCAG CATTCCCTAT GAAGGATTTG CTTCTCTCCG AGGGAGTAAT 
GGCCCAAGAA GATTCTGCGT GGAGAAATGG GGGAAAATCA CCGCTCTTCC CAGAGCTCAC 
ACTTGTTTTA ACCGTCTGGA TCTCCCTCCA TACCCATCCT TCTCCATGCT TTATGAAAAA 
CTTTTGACAG CAGTTGAGGA GACCAGTACC TTTGGACTTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW2 ( XM_021074544.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020930203.1 Sus scrofa HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OSe01255
Clone ID Related Accession (Same CDS sequence)	XM_021074541.1 , XM_021074543.1 , NM_001198924.1 , XM_021074542.1 , XM_021074545.1 , XM_021074544.1
Accession Version	XM_021074544.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Sus scrofa(pig)
Product	E3 ubiquitin-protein ligase HECW2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010457.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAGCT CAGCCCGGGA GCACCTGCTT TTTGTGCGGC GGCGAAATCC CCAGATGCGG 
TACACTCTGA GTCCAGAGAA CCTCCAGAGC CTGGCTGCCC AGAGCTCCAT GCCTGAGAAC 
ATGACCCTGC AGCGGGCCAA CAGCGACACA GATCTGGTGA CTTCTGAGAG CCGGTCCAGC 
CTGACTGCCA GCATGTATGA GTACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAAG AGGAGGTGGA CCCGAGTGAT TGGATTGGAC TTTATCACAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAAAAC AGGGGTGTGA CTGGAACACA AAAAGGACAA 
ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAGC CGGAGATAAA AATCTGTTTC 
AAATACTACC ACGGCATTAG TGGAGCCCTG CGAGCCACGA CCCCCTGCAT CACGGTGAAG 
AACCCTGCTG TGATGATGGG GGCAGAAGGC ATGGAAGGAG GTGCTGCAGG CAGTCAGCAT 
TCTCGGAAAC TCGTTAGCTT TACGTTGTCA GATCTTAGGG CAGTTGGGCT AAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTGAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT 
TTCCCCACCT GTGCCCACCA TGGGCAGGAG AGGAGGTCTA CCATCATCAG TAACACCACT 
AATCCAATCT GGCACCGAGA GAAATACTCC TTTTTTGCAC TCCTTACTGA TGTCTTAGAA 
ATTGAAATTA AAGACAAATT TGCCAAGAGC CGTCCTATCA TCAAGCGTTT TCTGGGGAAA 
CTAACCATCC CAGTCCAGAG GCTGTTGGAG CGACAAGCCA TCGGTGATCA GATGCTCAGC 
TACAACCTTG GCAGAAGGCT CCCAGCTGAC CACGTGAGTG GGTACCTCCA GTTCAGAGTG 
GAGGTCACGT CTTCTGTGCA TGAAGATGCT TCTCCAGAAG CAGTTGGCAC AATACTTGGA 
GTCAATACTG TGAATGGAGA CCTAGGAAGC CCTTCTGATG AGGAGGACAT GCCAGGGGGC 
CACCACGACA GCCCGGTTTG TTCTAATGGA CCAGTTTCTG AGGAAAGTGC TGATGGGACC 
CCCAAGCATT CTTTCAGGAC TAGCTCTACT CTGGAAATAG ACACGGAAGA TTTAACCTCT 
ACTTCTTCAA GGACCTCACC TCCCAGAGGA CGTCAGGATT CCCTCAACGA TTATTTAGAT 
GCTATTGAAC ACAATGGCCA TTCCAGGTCA GGTGCAGCTA CCTGCTCAGA GAGGTCCATG 
GGAGCCTCTC CAAAACTAAG GAGTAGTTTT CCCACCGACA CGAGACTCAA TGCCATGCTT 
CATATTGATT CAGATGAGGA AGACCACGAG TTCCAGCAGG ACCTGGGTTA TCCATCTTCC 
TTGGAGGAGG AAGGAGGGTT GATAATGTTT AGCAGGGCAT CAAGAACTGA TGACGGGAGC 
CTCACATCTC AGACAAAGCC GGAAGACATC CCAGTGGAGA CTGAGGAAGC CTCTATCCAC 
GAACCTGCTT CCTTTGAGGA TAAGCCAGAA CATCTTCCGG AGCTTACAGA GAGCTCCTTA 
CCCTCAGGCC CCATGCCAGA TGAAAGTGAA AATGGCCTGG AGTCTCAACC AGGTGCCGAC 
CAGGGCAGCA CTGAATTGTG TGGCTCTCAG GAGGTCGATC AGCCTACGAG TGGGGCAGAC 
ACGGGCACTT CTGACACGTC GGGAGGAAGC AGAAGAGCCA TCAGTGAGAC AGAGTCCCTT 
GATCAGGGGT CCGAGCCTTC CCAGGTGTCC TCTGAAACAG AACCCAGTGA CCCTGCCAGG 
ACAGAGAGCG TGAGTGAGGC CAGCACCAGG CCTGAGGGAG AGAGTGACCT GGAAGGGGCC 
GACAGCTCCT GCAATGAGAG TGTCACCACG CAGCTGTCCT CGGTGGAGAC ACGGTGCTCA 
TCTCTTGAGA GTGCACGGTT TCCCGAAACC CCAGCCTTTT CTTCTCAGGA GGAGGAAGAT 
GGAGCCTGTG CAGCAGAGCC CACCAGCAGT GGCCCTGCCG AGGGGTCACA GGATTCCATA 
TGCACTCCTG GGTCCTTACC TGTGGTGCAG GTGCCCAGTG GAGAGGAGGA AGGGCCAGGG 
GCAGAATCGG CCACCTTACC TGACCAGGAG GAGGTGGGGG AGGTCTGGCA GCGGAGGGGG 
AGCCTGGAGG GAGCTGCTGC TGCTGCTGCT GCTGCTGAGA GCCAGCCCCA AGAAGAGGGT 
GATGCTGGGG AAGCCCAAGG GGCTTGTGAA GGGGCCACTG CCCAGGAGGA GGGTGCTACT 
GGAGGTTCTC AAGCCAACGG CCACCAGCCA CTGCGATCTC TGCCTTCAGT GCGCCAAGAT 
GTTAGCCGGT ACCAGAGGGT GGACGAGGCT CTCCCACCAA ACTGGGAGGC CCGCATTGAC 
AGCCACGGCA GGATCTTCTA CGTGGATCAC GTGAACAGAA CCACGACGTG GCAGCGGCCC 
ACCGCTCCCC CCGCCCCGCA GGTGCTGCAG AGGTCCAACT CCATACAGCA GATGGAGCAG 
CTGAACCGGC GATACCAGAG TATCCGCAGA ACCATGACTA ACGAGAGGCC CGAGGAAAAT 
AGCAGTGCTC TTGATGGGGC AGGAGAGGAG GCCGACTTTC ACCAAGCCAG TGCAGATTTC 
CGACGGGAGA GCGTCCTGCC CCACTCTACC TCCAGATCCA GGCTCACGTT GCTGTTACAG 
TCGCCCCCTG TGAAGTTCCT CATCAGCCCA GAGTTCTTCA CCGTGCTGCA TTCGAACCCT 
AGTGCCTACC GCATGTTTAC AAACAACACG TGTTTGAAGC ACATGATCAC CAAAGTCCGG 
CGGGACACCC ACCACTTTGA ACGCTACCAG CATAACCGGG ACCTTGTGGG ATTCCTCAAC 
ATGTTTGCAA ACAAACAACT GGAGCTGCCG AGGGGCTGGG AAATGAAACA CGATCATCAG 
GGCAAGGCAT TTTTTGTTGA CCACAACTCC CGGACCACCA CTTTCATTGA CCCCCGGCTC 
CCACTCCAGA GCAGCAGACC CACGAGTGCG CTGGTGCATC GACAGCATCT GACGAGGCAG 
CGCAGCCACA GTGCAGGCGA GGTGGGAGAA GATTCTCGAC ATGCCGGACC ACCAGTTCTT 
CCCAGGCCGT CGAGTACGTT CAACACAGTC AGCAGGCCGC AGTACCAGGA CATGGTTCCA 
GTGGCTTACA ATGACAAGAT TGTTGCATTT CTTCGCCAAC CCAACATCTT TGAAATTCTA 
CAGGAGCGTC AACCTGATCT TACCAGGAAT CACTCACTCA GGGAGAAGAT CCAATTTATC 
CGAACTGAAG GGACCCCAGG ATTGGTGCGT CTTTCAAGTG ATGCAGACCT TGTTATGTTG 
CTAAGTTTAT TTGAAGAAGA GATAATGTCA TACGTGCCTC CACATGCCTT ACTCCATCCC 
AGCTACTGTC AGTCCCCACG TGGCTCCCCT GTGTCCTCTC CCCAGAACTC GCCAGGTACT 
CAGCGTGCCA ACGCCCGGGC TCCCGCCCCT TACAAGCGAG ACTTTGAAGC CAAACTGAGG 
AATTTTTACA GGAAGTTAGA GACTAAAGGA TATGGACAAG GCCCAGGGAA ATTAAAGTTA 
ATTATCAGAA GAGATCACTT GCTCGAAGAT GCTTTTAATC AGATTATGGG CTATTCCAGA 
AAAGACCTGC AGAGAAATAA GCTGTACGTC ACATTTGTCG GGGAGGAAGG GCTGGATTAC 
AGTGGACCTT CCAGAGAGTT TTTCTTCCTG GTATCCAGAG AGCTCTTTAA CCCATATTAC 
GGCTTATTTG AATATTCAGC CAATGACACA TACACAGTAC AAATAAGTCC CATGTCTGCT 
TTTGTAGACA ATCACCATGA ATGGTTCCGG TTCAGTGGTA GGATCCTTGG TCTTGCGCTG 
ATTCACCAGT ATTTGTTGGA TGCCTTCTTC ACGCGGCCCT TCTATAAGGC TCTTCTCAGA 
ATTCTGTGTG ACCTGAGTGA TCTAGAATAC CTTGATGAGG AGTTCCACCA GAGCCTGCAG 
TGGATGAAAG ACAATGATAT CCATGACATT CTGGACCTCA CGTTCACAGT GAACGAAGAA 
GTTTTCGGGC AGATAACTGA ACGAGAATTA AAACCAGGGG GTGCCAACAT CCCAGTCACA 
GAGAAGAACA AGAAGGAGTA CATCGAGAGG ATGGTGAAAT GGAGGATTGA GCGGGGTGTT 
GTGCAGCAAA CGGAGAGCTT AGTGCGTGGC TTCTACGAGG TGGTGGATGC CAGGCTAGTA 
TCTGTTTTTG ATGCAAGAGA ACTGGAACTG GTCATCGCAG GCACAGCTGA AATAGACCTA 
AGTGATTGGA GAAACAACAC AGAATACAGA GGAGGTTATC ATGACAATCA TATTGTAATT 
CGGTGGTTCT GGGCTGCAGT GGAGAGATTC AACAATGAAC AACGATTAAG GTTGTTACAG 
TTTGTCACAG GCACATCCAG CATTCCCTAT GAAGGATTTG CTTCTCTCCG AGGGAGTAAT 
GGCCCAAGAA GATTCTGCGT GGAGAAATGG GGGAAAATCA CCGCTCTTCC CAGAGCTCAC 
ACTTGTTTTA ACCGTCTGGA TCTCCCTCCA TACCCATCCT TCTCCATGCT TTATGAAAAA 
CTTTTGACAG CAGTTGAGGA GACCAGTACC TTTGGACTTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020930203.1
CDS	358..5082
Translation	MASSAREHLLFVRRRNPQMRYTLSPENLQSLAAQSSMPENMTLQRANSDTDLVTSESRSSLTASMYEYTLGQAQNLIIFWDIKEEVDPSDWIGLYHIDENSPANFWDSKNRGVTGTQKGQIVWRIEPGPYFMEPEIKICFKYYHGISGALRATTPCITVKNPAVMMGAEGMEGGAAGSQHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTCAHHGQERRSTIISNTTNPIWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAIGDQMLSYNLGRRLPADHVSGYLQFRVEVTSSVHEDASPEAVGTILGVNTVNGDLGSPSDEEDMPGGHHDSPVCSNGPVSEESADGTPKHSFRTSSTLEIDTEDLTSTSSRTSPPRGRQDSLNDYLDAIEHNGHSRSGAATCSERSMGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMFSRASRTDDGSLTSQTKPEDIPVETEEASIHEPASFEDKPEHLPELTESSLPSGPMPDESENGLESQPGADQGSTELCGSQEVDQPTSGADTGTSDTSGGSRRAISETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDLEGADSSCNESVTTQLSSVETRCSSLESARFPETPAFSSQEEEDGACAAEPTSSGPAEGSQDSICTPGSLPVVQVPSGEEEGPGAESATLPDQEEVGEVWQRRGSLEGAAAAAAAAESQPQEEGDAGEAQGACEGATAQEEGATGGSQANGHQPLRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENSSALDGAGEEADFHQASADFRRESVLPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNIFEILQERQPDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_021074544.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021074544.1

ORF Insert Sequence:

ATGGCTAGCT CAGCCCGGGA GCACCTGCTT TTTGTGCGGC GGCGAAATCC CCAGATGCGG 
TACACTCTGA GTCCAGAGAA CCTCCAGAGC CTGGCTGCCC AGAGCTCCAT GCCTGAGAAC 
ATGACCCTGC AGCGGGCCAA CAGCGACACA GATCTGGTGA CTTCTGAGAG CCGGTCCAGC 
CTGACTGCCA GCATGTATGA GTACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAAG AGGAGGTGGA CCCGAGTGAT TGGATTGGAC TTTATCACAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAAAAC AGGGGTGTGA CTGGAACACA AAAAGGACAA 
ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAGC CGGAGATAAA AATCTGTTTC 
AAATACTACC ACGGCATTAG TGGAGCCCTG CGAGCCACGA CCCCCTGCAT CACGGTGAAG 
AACCCTGCTG TGATGATGGG GGCAGAAGGC ATGGAAGGAG GTGCTGCAGG CAGTCAGCAT 
TCTCGGAAAC TCGTTAGCTT TACGTTGTCA GATCTTAGGG CAGTTGGGCT AAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTGAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT 
TTCCCCACCT GTGCCCACCA TGGGCAGGAG AGGAGGTCTA CCATCATCAG TAACACCACT 
AATCCAATCT GGCACCGAGA GAAATACTCC TTTTTTGCAC TCCTTACTGA TGTCTTAGAA 
ATTGAAATTA AAGACAAATT TGCCAAGAGC CGTCCTATCA TCAAGCGTTT TCTGGGGAAA 
CTAACCATCC CAGTCCAGAG GCTGTTGGAG CGACAAGCCA TCGGTGATCA GATGCTCAGC 
TACAACCTTG GCAGAAGGCT CCCAGCTGAC CACGTGAGTG GGTACCTCCA GTTCAGAGTG 
GAGGTCACGT CTTCTGTGCA TGAAGATGCT TCTCCAGAAG CAGTTGGCAC AATACTTGGA 
GTCAATACTG TGAATGGAGA CCTAGGAAGC CCTTCTGATG AGGAGGACAT GCCAGGGGGC 
CACCACGACA GCCCGGTTTG TTCTAATGGA CCAGTTTCTG AGGAAAGTGC TGATGGGACC 
CCCAAGCATT CTTTCAGGAC TAGCTCTACT CTGGAAATAG ACACGGAAGA TTTAACCTCT 
ACTTCTTCAA GGACCTCACC TCCCAGAGGA CGTCAGGATT CCCTCAACGA TTATTTAGAT 
GCTATTGAAC ACAATGGCCA TTCCAGGTCA GGTGCAGCTA CCTGCTCAGA GAGGTCCATG 
GGAGCCTCTC CAAAACTAAG GAGTAGTTTT CCCACCGACA CGAGACTCAA TGCCATGCTT 
CATATTGATT CAGATGAGGA AGACCACGAG TTCCAGCAGG ACCTGGGTTA TCCATCTTCC 
TTGGAGGAGG AAGGAGGGTT GATAATGTTT AGCAGGGCAT CAAGAACTGA TGACGGGAGC 
CTCACATCTC AGACAAAGCC GGAAGACATC CCAGTGGAGA CTGAGGAAGC CTCTATCCAC 
GAACCTGCTT CCTTTGAGGA TAAGCCAGAA CATCTTCCGG AGCTTACAGA GAGCTCCTTA 
CCCTCAGGCC CCATGCCAGA TGAAAGTGAA AATGGCCTGG AGTCTCAACC AGGTGCCGAC 
CAGGGCAGCA CTGAATTGTG TGGCTCTCAG GAGGTCGATC AGCCTACGAG TGGGGCAGAC 
ACGGGCACTT CTGACACGTC GGGAGGAAGC AGAAGAGCCA TCAGTGAGAC AGAGTCCCTT 
GATCAGGGGT CCGAGCCTTC CCAGGTGTCC TCTGAAACAG AACCCAGTGA CCCTGCCAGG 
ACAGAGAGCG TGAGTGAGGC CAGCACCAGG CCTGAGGGAG AGAGTGACCT GGAAGGGGCC 
GACAGCTCCT GCAATGAGAG TGTCACCACG CAGCTGTCCT CGGTGGAGAC ACGGTGCTCA 
TCTCTTGAGA GTGCACGGTT TCCCGAAACC CCAGCCTTTT CTTCTCAGGA GGAGGAAGAT 
GGAGCCTGTG CAGCAGAGCC CACCAGCAGT GGCCCTGCCG AGGGGTCACA GGATTCCATA 
TGCACTCCTG GGTCCTTACC TGTGGTGCAG GTGCCCAGTG GAGAGGAGGA AGGGCCAGGG 
GCAGAATCGG CCACCTTACC TGACCAGGAG GAGGTGGGGG AGGTCTGGCA GCGGAGGGGG 
AGCCTGGAGG GAGCTGCTGC TGCTGCTGCT GCTGCTGAGA GCCAGCCCCA AGAAGAGGGT 
GATGCTGGGG AAGCCCAAGG GGCTTGTGAA GGGGCCACTG CCCAGGAGGA GGGTGCTACT 
GGAGGTTCTC AAGCCAACGG CCACCAGCCA CTGCGATCTC TGCCTTCAGT GCGCCAAGAT 
GTTAGCCGGT ACCAGAGGGT GGACGAGGCT CTCCCACCAA ACTGGGAGGC CCGCATTGAC 
AGCCACGGCA GGATCTTCTA CGTGGATCAC GTGAACAGAA CCACGACGTG GCAGCGGCCC 
ACCGCTCCCC CCGCCCCGCA GGTGCTGCAG AGGTCCAACT CCATACAGCA GATGGAGCAG 
CTGAACCGGC GATACCAGAG TATCCGCAGA ACCATGACTA ACGAGAGGCC CGAGGAAAAT 
AGCAGTGCTC TTGATGGGGC AGGAGAGGAG GCCGACTTTC ACCAAGCCAG TGCAGATTTC 
CGACGGGAGA GCGTCCTGCC CCACTCTACC TCCAGATCCA GGCTCACGTT GCTGTTACAG 
TCGCCCCCTG TGAAGTTCCT CATCAGCCCA GAGTTCTTCA CCGTGCTGCA TTCGAACCCT 
AGTGCCTACC GCATGTTTAC AAACAACACG TGTTTGAAGC ACATGATCAC CAAAGTCCGG 
CGGGACACCC ACCACTTTGA ACGCTACCAG CATAACCGGG ACCTTGTGGG ATTCCTCAAC 
ATGTTTGCAA ACAAACAACT GGAGCTGCCG AGGGGCTGGG AAATGAAACA CGATCATCAG 
GGCAAGGCAT TTTTTGTTGA CCACAACTCC CGGACCACCA CTTTCATTGA CCCCCGGCTC 
CCACTCCAGA GCAGCAGACC CACGAGTGCG CTGGTGCATC GACAGCATCT GACGAGGCAG 
CGCAGCCACA GTGCAGGCGA GGTGGGAGAA GATTCTCGAC ATGCCGGACC ACCAGTTCTT 
CCCAGGCCGT CGAGTACGTT CAACACAGTC AGCAGGCCGC AGTACCAGGA CATGGTTCCA 
GTGGCTTACA ATGACAAGAT TGTTGCATTT CTTCGCCAAC CCAACATCTT TGAAATTCTA 
CAGGAGCGTC AACCTGATCT TACCAGGAAT CACTCACTCA GGGAGAAGAT CCAATTTATC 
CGAACTGAAG GGACCCCAGG ATTGGTGCGT CTTTCAAGTG ATGCAGACCT TGTTATGTTG 
CTAAGTTTAT TTGAAGAAGA GATAATGTCA TACGTGCCTC CACATGCCTT ACTCCATCCC 
AGCTACTGTC AGTCCCCACG TGGCTCCCCT GTGTCCTCTC CCCAGAACTC GCCAGGTACT 
CAGCGTGCCA ACGCCCGGGC TCCCGCCCCT TACAAGCGAG ACTTTGAAGC CAAACTGAGG 
AATTTTTACA GGAAGTTAGA GACTAAAGGA TATGGACAAG GCCCAGGGAA ATTAAAGTTA 
ATTATCAGAA GAGATCACTT GCTCGAAGAT GCTTTTAATC AGATTATGGG CTATTCCAGA 
AAAGACCTGC AGAGAAATAA GCTGTACGTC ACATTTGTCG GGGAGGAAGG GCTGGATTAC 
AGTGGACCTT CCAGAGAGTT TTTCTTCCTG GTATCCAGAG AGCTCTTTAA CCCATATTAC 
GGCTTATTTG AATATTCAGC CAATGACACA TACACAGTAC AAATAAGTCC CATGTCTGCT 
TTTGTAGACA ATCACCATGA ATGGTTCCGG TTCAGTGGTA GGATCCTTGG TCTTGCGCTG 
ATTCACCAGT ATTTGTTGGA TGCCTTCTTC ACGCGGCCCT TCTATAAGGC TCTTCTCAGA 
ATTCTGTGTG ACCTGAGTGA TCTAGAATAC CTTGATGAGG AGTTCCACCA GAGCCTGCAG 
TGGATGAAAG ACAATGATAT CCATGACATT CTGGACCTCA CGTTCACAGT GAACGAAGAA 
GTTTTCGGGC AGATAACTGA ACGAGAATTA AAACCAGGGG GTGCCAACAT CCCAGTCACA 
GAGAAGAACA AGAAGGAGTA CATCGAGAGG ATGGTGAAAT GGAGGATTGA GCGGGGTGTT 
GTGCAGCAAA CGGAGAGCTT AGTGCGTGGC TTCTACGAGG TGGTGGATGC CAGGCTAGTA 
TCTGTTTTTG ATGCAAGAGA ACTGGAACTG GTCATCGCAG GCACAGCTGA AATAGACCTA 
AGTGATTGGA GAAACAACAC AGAATACAGA GGAGGTTATC ATGACAATCA TATTGTAATT 
CGGTGGTTCT GGGCTGCAGT GGAGAGATTC AACAATGAAC AACGATTAAG GTTGTTACAG 
TTTGTCACAG GCACATCCAG CATTCCCTAT GAAGGATTTG CTTCTCTCCG AGGGAGTAAT 
GGCCCAAGAA GATTCTGCGT GGAGAAATGG GGGAAAATCA CCGCTCTTCC CAGAGCTCAC 
ACTTGTTTTA ACCGTCTGGA TCTCCCTCCA TACCCATCCT TCTCCATGCT TTATGAAAAA 
CTTTTGACAG CAGTTGAGGA GACCAGTACC TTTGGACTTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Knobbed acrosome defect is associated with a region containing the genes STK17b and HECW2 on porcine chromosome 15.
BMC genomics11699(2010 Dec)
Sironen A,Uimari P,Nagy S,Paku S,Andersson M,Vilkki J

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HECW2 cDNA ORF clone, Sus scrofa(pig)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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