GJA1 cDNA ORF clone, Sus scrofa(pig)

Gene
Clones
gRNAs

Summary

Gene Symbol	GJA1
Entrez Gene ID	100518636
Full Name	gap junction protein alpha 1
Synonyms	CX43
Gene Type	protein-coding
Organism	Sus scrofa(pig)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001244212.1	NP_001231141.1	gap junction alpha-1 protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Localization and quantitative analysis of Cx43 in porcine oocytes during in vitro maturation.
Lee S, Hiradate Y, Hoshino Y, Ko YG, Tanemura K, Sato E
Zygote (Cambridge, England)24(3)364-70(2016 Jun)

Influence of the antiandrogen flutamide on connexin 43 (Cx43) gene and protein expression in the porcine placenta and uterus during pregnancy.
Wieciech I, Grzesiak M, Knapczyk-Stwora K, Pytlik A, S?omczynska M
Folia biologica62(4)367-75(2014)

Androgen signaling disruption during fetal and postnatal development affects androgen receptor and connexin 43 expression and distribution in adult boar prostate.
Hejmej A, G?rowska E, Kotula-Balak M, Chojnacka K, Zarzycka M, Zaj?c J, Bili?ska B
BioMed research international2013407678(2013)

The dynamics of connexin expression, degradation and localisation are regulated by gonadotropins during the early stages of in vitro maturation of swine oocytes.
Santiquet N, Robert C, Richard FJ
PloS one8(7)e68456(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that connexin 43 (Cx43) is localized in the ooplasmic membrane through zona pellucidae and its level changes over time during culture in porcine oocytes.
Title: Localization and quantitative analysis of Cx43 in porcine oocytes during in vitro maturation.

The effects of flutamide on connexin 43 expression in porcine placenta and uterus throughout pregnancy are reported.
Title: Influence of the antiandrogen flutamide on connexin 43 (Cx43) gene and protein expression in the porcine placenta and uterus during pregnancy.

The in vitro cultivation of cumulus cells was associated with cell proliferation and that Cx43 and Cdk4 gene expression was upregulated after in vitro cultivation, resulting in significantly higher protein levels.
Title: Real-time proliferation of porcine cumulus cells is related to the protein levels and cellular distribution of Cdk4 and Cx43.

we demonstrated that modulation of Cx43 expression in the prostate could serve as a sensitive marker of hormonal disruption during different developmental stages.
Title: Androgen signaling disruption during fetal and postnatal development affects androgen receptor and connexin 43 expression and distribution in adult boar prostate.

Gonadotropins regulate Cx43 protein expression, degradation and localization in porcine cumulus oocyte complex.
Title: The dynamics of connexin expression, degradation and localisation are regulated by gonadotropins during the early stages of in vitro maturation of swine oocytes.

The following GJA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GJA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSe00597	NM_001244212.1 Latest version!	Sus scrofa gap junction protein alpha 1 (GJA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GJA1 ( NM_001244212.1 ) cDNA ORF clone, Sus scrofa(pig) -> NP_001231141.1 Sus scrofa gap junction protein alpha 1 (GJA1), mRNA.

Price & Availability↑

CloneID	OSe00597
Clone ID Related Accession (Same CDS sequence)	NM_001244212.1
Accession Version	NM_001244212.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Sus scrofa(pig)
Product	gap junction alpha-1 protein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from EV960972.2, DN129630.1, AJ293888.1, EW652303.2, DN129019.1, CF787394.1, EW180362.2 and BF703764.1. On or before Sep 15, 2011 this sequence version replaced XM_003121236.2, XM_003353229.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EV960972.2, DN115380.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1487420, SAMEA1876824 [ECO:0000348] ##Evidence-Data-END##

ATGGGTGACT GGAGTGCCTT AGGCAAACTC CTTGACAAGG TTCAAGCCTA TTCCACCGCT 
GGAGGGAAGG TGTGGCTGTC AGTCCTTTTC ATTTTCCGAA TCCTGCTCTT GGGCACAGCT 
GTTGAGTCAG CCTGGGGTGA TGAGCAGTCT GCCTTTCGTT GTAACACTCA GCAACCCGGT 
TGTGAAAATG TCTGCTATGA CAAATCCTTC CCCATCTCTC ACGTGCGTTT CTGGGTCCTG 
CAGATCATAT TTGTGTCTGT TCCTACACTC TTGTACCTGG CTCATGTGTT CTACGTGATG 
CGAAAGGAAG AGAAACTGAA CAAGAAAGAG GAGGAACTCA AAGTTGCCCA AACTGACGGT 
GTCAACGTGG AGATGCACTT AAAGCAGATA GAAATAAAGA AGTTTAAGTA CGGCATTGAG 
GAGCACGGCA AGGTGAAAAT GCGAGGGGGC TTGCTGCGAA CCTACATCAT CAGTATCCTC 
TTCAAGTCTG TCTTCGAGGT GGCCTTCTTG CTGATCCAAT GGTACATCTA TGGATTCAGC 
CTGAGTGCTG TTTACACTTG CAAAAGAGAT CCCTGCCCGC ACCAGGTGGA CTGTTTCCTC 
TCTCGTCCCA CGGAGAAAAC CATCTTCATC ATCTTCATGC TGGTCGTATC CTTGGTGTCT 
CTCGCCTTGA ACATCATCGA ACTCTTCTAT GTCTTCTTCA AGGGTGTTAA GGATCGTGTG 
AAGGGAAAGA GTGATCCTTA CCACGCCACC ACCGGTCCAC TGAGCCCCTC CAAAGACTGT 
GGATCTCCAA AATACGCTTA TTTCAATGGC TGCTCCTCAC CAACTGCTCC GCTCTCACCC 
ATGTCCCCTC CCGGATACAA GCTGGTTACC GGAGACAGAA ACAATTCTTC CTGCCGCAAT 
TACAACAAAC AAGCGAGTGA GCAAAACTGG GCTAATTACA GTGCCGAGCA GAATCGAATG 
GGGCAGGCAG GAAGCACCAT CTCTAACTCC CATGCACAGC CTTTTGATTT CCCCGATGAC 
AACCAGAATT CTAAAAAACT CGATGCTGGC CATGAACTCC AGCCTCTAGC CATTGTGGAC 
CAGCGGCCTT CCAGCAGAGC CAGCAGTCGC GCCAGCAGCC GACCTCGGCC TGATGACCTG 
GAGATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001231141.1
CDS	182..1330
Translation	MGDWSALGKLLDKVQAYSTAGGKVWLSVLFIFRILLLGTAVESAWGDEQSAFRCNTQQPGCENVCYDKSFPISHVRFWVLQIIFVSVPTLLYLAHVFYVMRKEEKLNKKEEELKVAQTDGVNVEMHLKQIEIKKFKYGIEEHGKVKMRGGLLRTYIISILFKSVFEVAFLLIQWYIYGFSLSAVYTCKRDPCPHQVDCFLSRPTEKTIFIIFMLVVSLVSLALNIIELFYVFFKGVKDRVKGKSDPYHATTGPLSPSKDCGSPKYAYFNGCSSPTAPLSPMSPPGYKLVTGDRNNSSCRNYNKQASEQNWANYSAEQNRMGQAGSTISNSHAQPFDFPDDNQNSKKLDAGHELQPLAIVDQRPSSRASSRASSRPRPDDLEI

Target ORF information:

RefSeq Version	NM_001244212.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa gap junction protein alpha 1 (GJA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001244212.1

ORF Insert Sequence:

ATGGGTGACT GGAGTGCCTT AGGCAAACTC CTTGACAAGG TTCAAGCCTA TTCCACCGCT 
GGAGGGAAGG TGTGGCTGTC AGTCCTTTTC ATTTTCCGAA TCCTGCTCTT GGGCACAGCT 
GTTGAGTCAG CCTGGGGTGA TGAGCAGTCT GCCTTTCGTT GTAACACTCA GCAACCCGGT 
TGTGAAAATG TCTGCTATGA CAAATCCTTC CCCATCTCTC ACGTGCGTTT CTGGGTCCTG 
CAGATCATAT TTGTGTCTGT TCCTACACTC TTGTACCTGG CTCATGTGTT CTACGTGATG 
CGAAAGGAAG AGAAACTGAA CAAGAAAGAG GAGGAACTCA AAGTTGCCCA AACTGACGGT 
GTCAACGTGG AGATGCACTT AAAGCAGATA GAAATAAAGA AGTTTAAGTA CGGCATTGAG 
GAGCACGGCA AGGTGAAAAT GCGAGGGGGC TTGCTGCGAA CCTACATCAT CAGTATCCTC 
TTCAAGTCTG TCTTCGAGGT GGCCTTCTTG CTGATCCAAT GGTACATCTA TGGATTCAGC 
CTGAGTGCTG TTTACACTTG CAAAAGAGAT CCCTGCCCGC ACCAGGTGGA CTGTTTCCTC 
TCTCGTCCCA CGGAGAAAAC CATCTTCATC ATCTTCATGC TGGTCGTATC CTTGGTGTCT 
CTCGCCTTGA ACATCATCGA ACTCTTCTAT GTCTTCTTCA AGGGTGTTAA GGATCGTGTG 
AAGGGAAAGA GTGATCCTTA CCACGCCACC ACCGGTCCAC TGAGCCCCTC CAAAGACTGT 
GGATCTCCAA AATACGCTTA TTTCAATGGC TGCTCCTCAC CAACTGCTCC GCTCTCACCC 
ATGTCCCCTC CCGGATACAA GCTGGTTACC GGAGACAGAA ACAATTCTTC CTGCCGCAAT 
TACAACAAAC AAGCGAGTGA GCAAAACTGG GCTAATTACA GTGCCGAGCA GAATCGAATG 
GGGCAGGCAG GAAGCACCAT CTCTAACTCC CATGCACAGC CTTTTGATTT CCCCGATGAC 
AACCAGAATT CTAAAAAACT CGATGCTGGC CATGAACTCC AGCCTCTAGC CATTGTGGAC 
CAGCGGCCTT CCAGCAGAGC CAGCAGTCGC GCCAGCAGCC GACCTCGGCC TGATGACCTG 
GAGATCTAG