LOC658667 cDNA ORF clone, Tribolium castaneum(red flour beetle)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC658667
Entrez Gene ID	658667
Full Name	probable citrate synthase 2, mitochondrial
Gene Type	protein-coding
Organism	Tribolium castaneum(red flour beetle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_965031.4	XP_970124.1	probable citrate synthase 2, mitochondrial

Pathways from BioSystems
Gene Ontology

The following LOC658667 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC658667 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTg07238	XM_965031.4 Latest version!	Tribolium castaneum probable citrate synthase 2, mitochondrial (LOC658667), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC658667 ( XM_965031.4 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_970124.1 Tribolium castaneum probable citrate synthase 2, mitochondrial (LOC658667), mRNA.

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CloneID	OTg07238
Clone ID Related Accession (Same CDS sequence)	XM_965031.4
Accession Version	XM_965031.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1401bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-20
Organism	Tribolium castaneum(red flour beetle)
Product	probable citrate synthase 2, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015450166.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 21, 2016 this sequence version replaced XM_965031.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACTGT TCCGTTTTAC AGCAACAAAA TTGGCCGAAA GTCAGAAAAT CGTCCCTCAA 
ATCTCGGTGG TTTTGCGACA ACTTAGCGAT GCCTCAACCG ATCTCAAAGC GGTCCTCGCC 
GCCAAAATCC CCAAAGAACA AGAACGTGTC AAAAACTTCC GTAAAAACTA CGGAAACCAG 
AAAGTCGGGG AAGTCACCGT CGATATGATG TATGGTGGTA TGAGAGGCAT TAAAGGCCTC 
GTGTGTGAAA CTTCGGTTCT CGACCCGGAC GAAGGTATCC GATTCCGAGG TCTGTCCATC 
CCCGAATGTC AGAAATTGTT ACCGAAAGCA CCCGGAGGCA GCGAACCCTT GCCCGAAGGG 
CTCTTCTGGT TGTTGATCAC CGGGGACGTA CCGACCGAAG CTCAGGTGAG GGCCATTTCG 
AAAGAGTGGG CCGAACGTGC AGAGTTACCG TCACATGTGG TCACACTGTT GAACAATTTG 
CCCACAAATG TGCACCCCAT GTCCCAGTTG TCGGCCGCTA TCACTGTACT GAACAGCGAA 
AGCAAGTTCG CTAAGGCGTA TTCAAGCGGT GTCCACAAGT CGAAATATTG GGAGTACGTT 
TACGAGGATT CCATGGACTT GATCGCGAAA TTGCCGGTGA TTGCGGCCAC TATCTACCGG 
AACACGTTCA AGGACGGGTC TGGGATCGGT GCCATAGACA CTTCCAAAGA CTGGAGTTAC 
AACTTCACGC AAATGCTGGG TTATGATAAC CCCGAATTTG TCGAACTTAT GCGACTATAT 
CTCACGATTC ACAGTGATCA TGAAGGTGGC AACGTTTCGG CCCACACTGT CCATTTGGTG 
GGGTCTGCTT TGAGCGACCC TTATTTGTCT TTTGCTGCCG GTATGAACGG ACTCGCTGGA 
CCCCTCCACG GTTTAGCCAA CCAAGAGGTG CTCCTGTGGT TGCAAAAAAT CCGCCAACAA 
ATCGGCGATA AAGCCACCGA AGAACAGCTC AAGGACTTCA TCTGGAAGAC GCTCAAATCG 
GGACAGGTTG TTCCAGGGTA CGGACACGCC GTTCTGCGTA AAACTGACCC CAGATACACG 
TGCCAGAGAG AATTTGCCTT GAAACACCTC CCCAACGATC CTCTCTTCCA GCTGGTGTCG 
AACATCTTCA AGGTGGTTCC CCCAATCCTG CTCGAGCTGG GCAAGGTGAA GAACCCGTGG 
CCCAACGTGG ACGCCCACTC CGGCGTGCTT TTGCAGTATT ACGGACTTAA AGAAATGAAT 
TATTACACTG TTTTGTTCGG GGTTTCCCGT GCATTGGGTG TGTTGGCATC ACTGGTCTGG 
GATCGGGCTC TCGGTCTGCC GATCGAAAGG CCGAAATCGT TGTCAACGGA TGGTCTCATG 
AAGGCGCTCA AGGCCGCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_970124.1
CDS	125..1525
Translation	MALFRFTATKLAESQKIVPQISVVLRQLSDASTDLKAVLAAKIPKEQERVKNFRKNYGNQKVGEVTVDMMYGGMRGIKGLVCETSVLDPDEGIRFRGLSIPECQKLLPKAPGGSEPLPEGLFWLLITGDVPTEAQVRAISKEWAERAELPSHVVTLLNNLPTNVHPMSQLSAAITVLNSESKFAKAYSSGVHKSKYWEYVYEDSMDLIAKLPVIAATIYRNTFKDGSGIGAIDTSKDWSYNFTQMLGYDNPEFVELMRLYLTIHSDHEGGNVSAHTVHLVGSALSDPYLSFAAGMNGLAGPLHGLANQEVLLWLQKIRQQIGDKATEEQLKDFIWKTLKSGQVVPGYGHAVLRKTDPRYTCQREFALKHLPNDPLFQLVSNIFKVVPPILLELGKVKNPWPNVDAHSGVLLQYYGLKEMNYYTVLFGVSRALGVLASLVWDRALGLPIERPKSLSTDGLMKALKAA

Target ORF information:

RefSeq Version	XM_965031.4
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum probable citrate synthase 2, mitochondrial (LOC658667), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_965031.4

ORF Insert Sequence:

ATGGCACTGT TCCGTTTTAC AGCAACAAAA TTGGCCGAAA GTCAGAAAAT CGTCCCTCAA 
ATCTCGGTGG TTTTGCGACA ACTTAGCGAT GCCTCAACCG ATCTCAAAGC GGTCCTCGCC 
GCCAAAATCC CCAAAGAACA AGAACGTGTC AAAAACTTCC GTAAAAACTA CGGAAACCAG 
AAAGTCGGGG AAGTCACCGT CGATATGATG TATGGTGGTA TGAGAGGCAT TAAAGGCCTC 
GTGTGTGAAA CTTCGGTTCT CGACCCGGAC GAAGGTATCC GATTCCGAGG TCTGTCCATC 
CCCGAATGTC AGAAATTGTT ACCGAAAGCA CCCGGAGGCA GCGAACCCTT GCCCGAAGGG 
CTCTTCTGGT TGTTGATCAC CGGGGACGTA CCGACCGAAG CTCAGGTGAG GGCCATTTCG 
AAAGAGTGGG CCGAACGTGC AGAGTTACCG TCACATGTGG TCACACTGTT GAACAATTTG 
CCCACAAATG TGCACCCCAT GTCCCAGTTG TCGGCCGCTA TCACTGTACT GAACAGCGAA 
AGCAAGTTCG CTAAGGCGTA TTCAAGCGGT GTCCACAAGT CGAAATATTG GGAGTACGTT 
TACGAGGATT CCATGGACTT GATCGCGAAA TTGCCGGTGA TTGCGGCCAC TATCTACCGG 
AACACGTTCA AGGACGGGTC TGGGATCGGT GCCATAGACA CTTCCAAAGA CTGGAGTTAC 
AACTTCACGC AAATGCTGGG TTATGATAAC CCCGAATTTG TCGAACTTAT GCGACTATAT 
CTCACGATTC ACAGTGATCA TGAAGGTGGC AACGTTTCGG CCCACACTGT CCATTTGGTG 
GGGTCTGCTT TGAGCGACCC TTATTTGTCT TTTGCTGCCG GTATGAACGG ACTCGCTGGA 
CCCCTCCACG GTTTAGCCAA CCAAGAGGTG CTCCTGTGGT TGCAAAAAAT CCGCCAACAA 
ATCGGCGATA AAGCCACCGA AGAACAGCTC AAGGACTTCA TCTGGAAGAC GCTCAAATCG 
GGACAGGTTG TTCCAGGGTA CGGACACGCC GTTCTGCGTA AAACTGACCC CAGATACACG 
TGCCAGAGAG AATTTGCCTT GAAACACCTC CCCAACGATC CTCTCTTCCA GCTGGTGTCG 
AACATCTTCA AGGTGGTTCC CCCAATCCTG CTCGAGCTGG GCAAGGTGAA GAACCCGTGG 
CCCAACGTGG ACGCCCACTC CGGCGTGCTT TTGCAGTATT ACGGACTTAA AGAAATGAAT 
TATTACACTG TTTTGTTCGG GGTTTCCCGT GCATTGGGTG TGTTGGCATC ACTGGTCTGG 
GATCGGGCTC TCGGTCTGCC GATCGAAAGG CCGAAATCGT TGTCAACGGA TGGTCTCATG 
AAGGCGCTCA AGGCCGCTTA A