LOC658809 cDNA ORF clone, Tribolium castaneum(red flour beetle)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC658809
Entrez Gene ID	658809
Full Name	ATP-dependent zinc metalloprotease YME1 homolog
Gene Type	protein-coding
Organism	Tribolium castaneum(red flour beetle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_965166.3	XP_970259.1	ATP-dependent zinc metalloprotease YME1 homolog

Pathways from BioSystems
Gene Ontology

The following LOC658809 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC658809 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTg09200	XM_965166.3 Latest version!	Tribolium castaneum ATP-dependent zinc metalloprotease YME1 homolog (LOC658809), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC658809 ( XM_965166.3 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_970259.1 Tribolium castaneum ATP-dependent zinc metalloprotease YME1 homolog (LOC658809), mRNA.

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CloneID	OTg09200
Clone ID Related Accession (Same CDS sequence)	XM_965166.3
Accession Version	XM_965166.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2151bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-20
Organism	Tribolium castaneum(red flour beetle)
Product	ATP-dependent zinc metalloprotease YME1 homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015450175.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Mar 21, 2016 this sequence version replaced XM_965166.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTTCAC TCCAGAACCA AAACCAGGCA ATCTTCACTC TCTCCCAAAT CTCCGCGATA 
ATAACCCCCC GTCAGAATGC CTTCCTGACC AAAAAAGCCC ACAAAGACAA ATCGCAAATC 
ATGTGCAAGG AAAGCTTCCT GGAGGCGCTC AAAAACTGCG ACTTCTCCTT CACCAAGCAC 
CTCAAAGACA CCTCATTCCT CAACAACTTC AAAGTCTCGT ACGTCTCGCA CGACACTTTC 
TTCGAAAACA AACACGGTTT CTCCGAAGCC GACTTAAAGA GCGCAGTTCC AACCCTCATA 
GTCAAAAATC GCAAACACTT CTACGTCCCC CAAAACTTCT ACCAGAAGCT AATCCACGGT 
TTGCGGCACG AAAACGCCTT CACGTTCGAC ATCCAAGTGC GGGGCTTCAA GACAGACCGC 
AGTGTCAAGG CGGAAATGCA GCGAAATCCG GGCCTGGGCG CCCGCATCAA AAACGCCCTA 
GGGCTGACCC CAAGCGAGAA TTTGGACTCG AAATTGGCCA AAGACTTGTC GTTGGGCAAC 
GAAAAGATGA AACAGATTTT CGCAGCCGAG GGGAATTCAA TGACAGAGGC GGAAAAACAA 
AGGATTAAGG TGGCTTTTGC CGAGGGCTAT CTCTTGGCCA ATAGCCCGGG GGCGCGGACG 
GGCAAAGCCG CGCGGTATTT TAAGGTGTTT CAGCAGGTGT TGACCATTGT GATCTTCCTA 
GCCATTGTTA TTAGTTTGAT GGTCAGTACA ACGGGCTCGA TTTTTAGAAT TCAATTAGGG 
AATCAAGTGG AGGTGGACCC TGAGGAGATA CATGTGACTT TTGATGACGT CAAGGGGGCT 
GATGAGGCCA AACAAGAGCT TAAAGACGTA GTTGAGTTTT TGAAAAATCC CGATAAATTT 
TCACAATTAG GGGGCAAACT ACCAAAGGGG GTGTTGTTAG TAGGGCCTCC AGGTACTGGT 
AAAACACTTC TAGCTAGGGC TGTGGCTGGT GAGGCGGGTG TGCCCTTTTT TCACGCTGCA 
GGGCCGGAAT TTGATGAAAT TTTGGTGGGG CAAGGGGCTA GGCGTGTCCG GGATTTATTT 
AAGTCTGCCA AAGAAAAGGC CCCCTGTGTG ATTTTCATCG ATGAAATCGA TTCAGTTGGT 
GCGAAACGGA CGAATAGTGT TTTACATCCG TATGCAAATC AAACGATTAA TCAACTTTTG 
AGCGAAATGG ACGGTTTTCA TCAAAACGAG GGGGTTATTG TTTTGGGGGC AACTAATCGA 
AGGGATGATT TAGATCAAGC CTTGCTGAGA CCGGGACGGT TTGATGTTGA GGTCACGGTC 
CCTACGCCCG ATTTCACCGG CCGGAAAGAA ATCCTGGGCC TCTATTTGGG CAAAGTCCTT 
GCCAAGGACG TGGATTTGGA ACTCTTGGCC CGGGGAACCA CCGGTTTTAC GGGCGCAGAC 
CTCGAAAACA TGGTCAATCA AGCTGCTCTC AAGGCTGCCA TCGATGGGGC CGATTGCGTT 
AGCATGAAAT ACCTCGAATC GGCGCGCGAT AAAGTCTTAA TGGGCCCCGA ACGCAAATCA 
CGAATTCCCG ACGAAGAAGC CAACCTTATA ACAGCGTACC ATGAAGGGGG CCACGCGATT 
GTGGCGTTCT ACACGCGTGA CAGCCATCCG TTGCATAAAG TTACGATAAT ACCGAGGGGG 
CCCAGTTTGG GGCACACGGC CTACATTCCG GAGAAGGAGC GCTATCATGT GACCAAATCG 
CAACTTTTGG CAACGATGGA TGTCATGATG GGGGGCAGAG CCGCCGAAGA GCTGATTTTC 
GGGACGGAAA AAATCACCTC AGGGGCGAGC TCGGATTTGA AGCAAGCGAC GTCCATAGCC 
ACCCATATGG TGAAGGACTG GGGCATGTCG GAGAAAATCG GCCTGAGGAC GATGACTGAA 
AGCTCGAAGC CGTTCCAAGG CGACAGTTTG GGGCCGTCGA CTAACGAACT CGTTGATAGC 
GAAATTAGGA GAATTCTGTC AGAAAGTTAC GACAGAGCCA AACATATTCT CAAAGCGCAT 
GCCAAGGAAC ATAAGGCCTT AGCCGAGGCT TTGATGAAGT ACGAGACGTT AGATGCGGAA 
GATATTAAAG CCATAATGAC AGAAAAAGGG CCACATGTTG AAAAATCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_970259.1
CDS	190..2340
Translation	MFSLQNQNQAIFTLSQISAIITPRQNAFLTKKAHKDKSQIMCKESFLEALKNCDFSFTKHLKDTSFLNNFKVSYVSHDTFFENKHGFSEADLKSAVPTLIVKNRKHFYVPQNFYQKLIHGLRHENAFTFDIQVRGFKTDRSVKAEMQRNPGLGARIKNALGLTPSENLDSKLAKDLSLGNEKMKQIFAAEGNSMTEAEKQRIKVAFAEGYLLANSPGARTGKAARYFKVFQQVLTIVIFLAIVISLMVSTTGSIFRIQLGNQVEVDPEEIHVTFDDVKGADEAKQELKDVVEFLKNPDKFSQLGGKLPKGVLLVGPPGTGKTLLARAVAGEAGVPFFHAAGPEFDEILVGQGARRVRDLFKSAKEKAPCVIFIDEIDSVGAKRTNSVLHPYANQTINQLLSEMDGFHQNEGVIVLGATNRRDDLDQALLRPGRFDVEVTVPTPDFTGRKEILGLYLGKVLAKDVDLELLARGTTGFTGADLENMVNQAALKAAIDGADCVSMKYLESARDKVLMGPERKSRIPDEEANLITAYHEGGHAIVAFYTRDSHPLHKVTIIPRGPSLGHTAYIPEKERYHVTKSQLLATMDVMMGGRAAEELIFGTEKITSGASSDLKQATSIATHMVKDWGMSEKIGLRTMTESSKPFQGDSLGPSTNELVDSEIRRILSESYDRAKHILKAHAKEHKALAEALMKYETLDAEDIKAIMTEKGPHVEKS

Target ORF information:

RefSeq Version	XM_965166.3
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum ATP-dependent zinc metalloprotease YME1 homolog (LOC658809), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_965166.3

ORF Insert Sequence:

ATGTTTTCAC TCCAGAACCA AAACCAGGCA ATCTTCACTC TCTCCCAAAT CTCCGCGATA 
ATAACCCCCC GTCAGAATGC CTTCCTGACC AAAAAAGCCC ACAAAGACAA ATCGCAAATC 
ATGTGCAAGG AAAGCTTCCT GGAGGCGCTC AAAAACTGCG ACTTCTCCTT CACCAAGCAC 
CTCAAAGACA CCTCATTCCT CAACAACTTC AAAGTCTCGT ACGTCTCGCA CGACACTTTC 
TTCGAAAACA AACACGGTTT CTCCGAAGCC GACTTAAAGA GCGCAGTTCC AACCCTCATA 
GTCAAAAATC GCAAACACTT CTACGTCCCC CAAAACTTCT ACCAGAAGCT AATCCACGGT 
TTGCGGCACG AAAACGCCTT CACGTTCGAC ATCCAAGTGC GGGGCTTCAA GACAGACCGC 
AGTGTCAAGG CGGAAATGCA GCGAAATCCG GGCCTGGGCG CCCGCATCAA AAACGCCCTA 
GGGCTGACCC CAAGCGAGAA TTTGGACTCG AAATTGGCCA AAGACTTGTC GTTGGGCAAC 
GAAAAGATGA AACAGATTTT CGCAGCCGAG GGGAATTCAA TGACAGAGGC GGAAAAACAA 
AGGATTAAGG TGGCTTTTGC CGAGGGCTAT CTCTTGGCCA ATAGCCCGGG GGCGCGGACG 
GGCAAAGCCG CGCGGTATTT TAAGGTGTTT CAGCAGGTGT TGACCATTGT GATCTTCCTA 
GCCATTGTTA TTAGTTTGAT GGTCAGTACA ACGGGCTCGA TTTTTAGAAT TCAATTAGGG 
AATCAAGTGG AGGTGGACCC TGAGGAGATA CATGTGACTT TTGATGACGT CAAGGGGGCT 
GATGAGGCCA AACAAGAGCT TAAAGACGTA GTTGAGTTTT TGAAAAATCC CGATAAATTT 
TCACAATTAG GGGGCAAACT ACCAAAGGGG GTGTTGTTAG TAGGGCCTCC AGGTACTGGT 
AAAACACTTC TAGCTAGGGC TGTGGCTGGT GAGGCGGGTG TGCCCTTTTT TCACGCTGCA 
GGGCCGGAAT TTGATGAAAT TTTGGTGGGG CAAGGGGCTA GGCGTGTCCG GGATTTATTT 
AAGTCTGCCA AAGAAAAGGC CCCCTGTGTG ATTTTCATCG ATGAAATCGA TTCAGTTGGT 
GCGAAACGGA CGAATAGTGT TTTACATCCG TATGCAAATC AAACGATTAA TCAACTTTTG 
AGCGAAATGG ACGGTTTTCA TCAAAACGAG GGGGTTATTG TTTTGGGGGC AACTAATCGA 
AGGGATGATT TAGATCAAGC CTTGCTGAGA CCGGGACGGT TTGATGTTGA GGTCACGGTC 
CCTACGCCCG ATTTCACCGG CCGGAAAGAA ATCCTGGGCC TCTATTTGGG CAAAGTCCTT 
GCCAAGGACG TGGATTTGGA ACTCTTGGCC CGGGGAACCA CCGGTTTTAC GGGCGCAGAC 
CTCGAAAACA TGGTCAATCA AGCTGCTCTC AAGGCTGCCA TCGATGGGGC CGATTGCGTT 
AGCATGAAAT ACCTCGAATC GGCGCGCGAT AAAGTCTTAA TGGGCCCCGA ACGCAAATCA 
CGAATTCCCG ACGAAGAAGC CAACCTTATA ACAGCGTACC ATGAAGGGGG CCACGCGATT 
GTGGCGTTCT ACACGCGTGA CAGCCATCCG TTGCATAAAG TTACGATAAT ACCGAGGGGG 
CCCAGTTTGG GGCACACGGC CTACATTCCG GAGAAGGAGC GCTATCATGT GACCAAATCG 
CAACTTTTGG CAACGATGGA TGTCATGATG GGGGGCAGAG CCGCCGAAGA GCTGATTTTC 
GGGACGGAAA AAATCACCTC AGGGGCGAGC TCGGATTTGA AGCAAGCGAC GTCCATAGCC 
ACCCATATGG TGAAGGACTG GGGCATGTCG GAGAAAATCG GCCTGAGGAC GATGACTGAA 
AGCTCGAAGC CGTTCCAAGG CGACAGTTTG GGGCCGTCGA CTAACGAACT CGTTGATAGC 
GAAATTAGGA GAATTCTGTC AGAAAGTTAC GACAGAGCCA AACATATTCT CAAAGCGCAT 
GCCAAGGAAC ATAAGGCCTT AGCCGAGGCT TTGATGAAGT ACGAGACGTT AGATGCGGAA 
GATATTAAAG CCATAATGAC AGAAAAAGGG CCACATGTTG AAAAATCTTA A