LOC660100 cDNA ORF clone, Tribolium castaneum(red flour beetle)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC660100
Entrez Gene ID	660100
Full Name	pleckstrin homology-like domain family B member 1
Gene Type	protein-coding
Organism	Tribolium castaneum(red flour beetle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008193048.2	XP_008191270.1	pleckstrin homology-like domain family B member 1 isoform X1
XM_015978278.1	XP_015833764.1	pleckstrin homology-like domain family B member 1 isoform X1
XM_966360.3	XP_971453.1	pleckstrin homology-like domain family B member 1 isoform X2
XM_015978279.1	XP_015833765.1	pleckstrin homology-like domain family B member 1 isoform X3

Pathways from BioSystems
Gene Ontology

The following LOC660100 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC660100 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTg17839	XM_015978279.1 Latest version!	Tribolium castaneum pleckstrin homology-like domain family B member 1 (LOC660100), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OTg03799	XM_015978278.1 Latest version!	Tribolium castaneum pleckstrin homology-like domain family B member 1 (LOC660100), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OTg03799	XM_008193048.2 Latest version!	Tribolium castaneum pleckstrin homology-like domain family B member 1 (LOC660100), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OTg03800	XM_966360.3 Latest version!	Tribolium castaneum pleckstrin homology-like domain family B member 1 (LOC660100), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC660100 ( XM_015978279.1 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_015833765.1 Tribolium castaneum pleckstrin homology-like domain family B member 1 (LOC660100), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OTg17839
Clone ID Related Accession (Same CDS sequence)	XM_015978279.1
Accession Version	XM_015978279.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-20
Organism	Tribolium castaneum(red flour beetle)
Product	pleckstrin homology-like domain family B member 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015450149.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGACCA TCGGCCGATC AAACTACATG CGTTTTAACC ACCCGGCGGA AGCAAAAATA 
ATGAAATCAG TTCTCCCCAA CCCTCGCATC TCCATGGCTT CGATCACTTT CGAACCCGAC 
AATTCTTTTC AGTCTAAATA CAACAAAAAA CCACCAGCAG TGCCTCGAAA AAACTCGAAA 
GAATCCCTCA TTGAATCGGG TGTCGAAGAA CCGGTGCCTT CCAGTATTAT GACCAAAGTG 
TCAAAATTCG AGTATTTAGC CCAACAAAAT TTCAAAAAAA GCATATCACC GAAAGTGTTC 
TCTTCAAATC TCGTAACTGT AAATACGCCG GCGAAAGACG TCCTCGGAAA ATCGCCACCT 
GATCTGCAAA ACTTCAGCAA GAATTTACCA CAAAATGCCT TAAACTACTC CGAACTGAAT 
TACAACGAAA AACAACAAAT TAAAACATCG GAGCGGTTTG GCAAAAAGTC GCCGCAGTAC 
GTTAACGTGA CGGTGAGCGA GAAGAAAAAC ATTAATAATA AAGTGATCAT TTACGAAAAT 
GGATGTATTC CGAAAAACCC AAACCTCGAA TGCAACGAAC TGAATAATAA AAGCTCAAGC 
CACAAAAATA TTAACATGAA TCGAGCGCTC ACACCATCGC CCAACTATAA CAGGAATCCT 
TCGCCACATT TGAGGAGCAC AACGCCGAAT AATTGTAAGA ACGAAACCGA AATTACAGAA 
AACTGCAACG ACGCTGAACT GAAAAGGACA CAGGCGCAGC AAGAACGAAT CAAGGAGCAA 
GAAATCGGGA AAGCGGAACA GGCAAGACTT GAAGAAATTC TAAATATGTG CGTTGAATAC 
GAAAAGCAGG CACAATCGGA AAAAAATAAA CTCACGCCGA ATAGAATAAA AACAAACGGT 
TCGCTGCCGC GTGATAAGCG TCAGTATGGA CCCTTATCTC CTGTGTATAC CACTCCTCCA 
CCTTCGCCGA GAGACGTGGA AATTGTTCAG CATGAGAACA GGAGGAATAA TTCTTCGTAT 
GAGAATATTA GTATTAATAG GGATAATTCA CTGGAACGAA TTAATGCCAA TTATGATAAT 
GTTGAAATGA AGCAAGTTGA TGACTTTAGG ACTACGAAGT GTAACAGTCC GTATGAAAAT 
ATTGTTCCGG CGCATCAATC GCCAAGGACG AGGATTAAGA CTATCGCTTC GTCGAACCGA 
GACGGCAAAT CTAAGGACAC TGCCGAAATA ATCGAAAACA AATTCGCCAT TCTCGAAGCC 
GAGCAATACC TCCTCCAGAA TCTCACAATA AACGACGAAA AATCTCTCGA AACCGACAAA 
ATTCTCGCTG GCCGAGAAAA CACAAAATTC ATATTTCCCC TCGAAGAAAA ATCCTTCACC 
TCACTCCCTG CCAATTTAAA CGCCACGCCA TACAACAAAA AACCAAAACC AAACAACATA 
AACGACAAAC AAAAAATCGA AAAAATCCGA AAAGAACGCA AAGAAATTCT CTCGCAAATC 
TCGTCCATCA AGCGCCACAT CGCCGAAACG GAAATCCACG AAGAGGAACT GCAGCGCGAC 
ATTGAGCTCG AAAACGCCCT CATTTCCGGC GAACACAAAG CAAAATTGCT CGAACTGGAC 
AAATTGGAAC ACCGCAAGAA CAAACTCTTC AACTGTGCGC AAAAACTGGA AGAGAGTATG 
TCACACCGAC AGACGAAACA AGACGAAGAT CAGTTAAAAT GCAAAGAAAA GCTCGAACTA 
GCGCAGGAAA ATATGCTTCA AATCGAAGGC AAGTTGGCCA AGACGCCCAA AACCGACCCC 
AAGTACGAGA ACGTCTTCGA GGAGTATTTG AAAGCTCAAG AGATTTTGGA CAACGAGAAG 
AAAGCGTTCG AAGACTTGGA GTTCCACCAT CTGGAGGAGG AAGCCGATTG GTTGGCCAGT 
CGGGAAGAGG TCCAACGGGA AATTCTGGAT TTGTCCAAAA AGATTGACTC GGTTAAAGAC 
CAAATCCAGG AATTGGAACA ACAAAAGTAC AACATCTCGA AGAACAACAA CACTGAAGTG 
AAGATCATTC GCATGCGACG TGACGAATTC ATGCGCAAGT TGGAGAAGTG TTCGTTCGTT 
TTGCGGCGAA TTGACAACGA TTTGTCCGTT TTCGCCCATC AGGAGTCCGA CCAAGAGGAA 
TCAACTGACA GCGACAGCGA CAAATCGAAA GACCTGGACA AACATTTGTC CAATCTATCG 
TTGAATGTGA TCCATGACCT GAGCTGTTCG GTCATCGTGA GCAACACCAA GACCTCAATT 
GACCACGTGT ACAACATGTC GCAGTCATTT AACGAAAAAT TGTTGCAAGA AAAATCGGTT 
TTGGAGACCG GGATTGGCAC CAAATGTCCA AGTCAGGATG ACATTGACCG GATCAGTAAA 
GTAACATCAA GTGCCCCTAT TAGTATCAAT GAGGAGCAAG GATCTCTGGG AAGAAAGACA 
ATCGAATCGC TTAAAGATAT CGAAAGGAAG AGACATCTTC ATTTGTGCCA ACAAGGTTCG 
CAGGTTATCG AGCAGGAGAG ACAAAGAGTG CTGGCTTTGA AACAGAGGGT GCAGCATGAA 
GTGAGGATCA AGTGGGCGCA GAGCAGGCAG GATTGTGTGG TTCCTGAGTC AGGAGAGAAG 
AGAAACAGTG TTGAAATCGT AGGACGACAA CCCGAAAGGC CCGTTTCAAA ATCTCCTACG 
TCAAACGAAC AGATACGCTC CGGCTCAAGT GAGGCGGATT CGCCACGTCC AATGTCTGAA 
ATTAGTGAAA TAAGCACCGA TTTCGGAGAA ATCTCAAACA AGAAAAGAAC AAAACCCCAT 
GGCGACAAAC AACGACCCTT AACCCGATAT TTGCCCATCA GAGGCTCAGA CCTCGACCTA 
CGCCAACATA TAGAATCGGC AGGTCATCAA GTGGTGTTAT GTCCCCACGT CATCATCAAT 
TCGAGCAGCT GCAGAGGCTT TTTGCACAAA AAAGGGTCAA AATTGAACGG ATGGTCGAAA 
AGGTGGTTCG TTTTCGATCG AAATAAGCAT ACGCTGACTT ACTACAACGA CAAGAGTGAA 
AAGAAAGCTC GTGGAGGAGC CTATTTTCAA GCGATTGAAG AGGTCTATTT GGACCACCTC 
AACAGTGTTA AATCGCCGAA CCCCCAACTG ACCTTCATCG TGAAAACGCA CGAAAAGTCC 
TATTATCTAC TGGCGCCCTC GCCCGAAGCG ATGAGGATTT GGGTTGATGT TATCTTTACT 
GGTGCTGAAG GGTACCAAGA ATTTGGACAT GGTACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015833765.1
CDS	96..3374
Translation	MLTIGRSNYMRFNHPAEAKIMKSVLPNPRISMASITFEPDNSFQSKYNKKPPAVPRKNSKESLIESGVEEPVPSSIMTKVSKFEYLAQQNFKKSISPKVFSSNLVTVNTPAKDVLGKSPPDLQNFSKNLPQNALNYSELNYNEKQQIKTSERFGKKSPQYVNVTVSEKKNINNKVIIYENGCIPKNPNLECNELNNKSSSHKNINMNRALTPSPNYNRNPSPHLRSTTPNNCKNETEITENCNDAELKRTQAQQERIKEQEIGKAEQARLEEILNMCVEYEKQAQSEKNKLTPNRIKTNGSLPRDKRQYGPLSPVYTTPPPSPRDVEIVQHENRRNNSSYENISINRDNSLERINANYDNVEMKQVDDFRTTKCNSPYENIVPAHQSPRTRIKTIASSNRDGKSKDTAEIIENKFAILEAEQYLLQNLTINDEKSLETDKILAGRENTKFIFPLEEKSFTSLPANLNATPYNKKPKPNNINDKQKIEKIRKERKEILSQISSIKRHIAETEIHEEELQRDIELENALISGEHKAKLLELDKLEHRKNKLFNCAQKLEESMSHRQTKQDEDQLKCKEKLELAQENMLQIEGKLAKTPKTDPKYENVFEEYLKAQEILDNEKKAFEDLEFHHLEEEADWLASREEVQREILDLSKKIDSVKDQIQELEQQKYNISKNNNTEVKIIRMRRDEFMRKLEKCSFVLRRIDNDLSVFAHQESDQEESTDSDSDKSKDLDKHLSNLSLNVIHDLSCSVIVSNTKTSIDHVYNMSQSFNEKLLQEKSVLETGIGTKCPSQDDIDRISKVTSSAPISINEEQGSLGRKTIESLKDIERKRHLHLCQQGSQVIEQERQRVLALKQRVQHEVRIKWAQSRQDCVVPESGEKRNSVEIVGRQPERPVSKSPTSNEQIRSGSSEADSPRPMSEISEISTDFGEISNKKRTKPHGDKQRPLTRYLPIRGSDLDLRQHIESAGHQVVLCPHVIINSSSCRGFLHKKGSKLNGWSKRWFVFDRNKHTLTYYNDKSEKKARGGAYFQAIEEVYLDHLNSVKSPNPQLTFIVKTHEKSYYLLAPSPEAMRIWVDVIFTGAEGYQEFGHGT

Target ORF information:

RefSeq Version	XM_015978279.1
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum pleckstrin homology-like domain family B member 1 (LOC660100), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015978279.1

ORF Insert Sequence:

ATGCTGACCA TCGGCCGATC AAACTACATG CGTTTTAACC ACCCGGCGGA AGCAAAAATA 
ATGAAATCAG TTCTCCCCAA CCCTCGCATC TCCATGGCTT CGATCACTTT CGAACCCGAC 
AATTCTTTTC AGTCTAAATA CAACAAAAAA CCACCAGCAG TGCCTCGAAA AAACTCGAAA 
GAATCCCTCA TTGAATCGGG TGTCGAAGAA CCGGTGCCTT CCAGTATTAT GACCAAAGTG 
TCAAAATTCG AGTATTTAGC CCAACAAAAT TTCAAAAAAA GCATATCACC GAAAGTGTTC 
TCTTCAAATC TCGTAACTGT AAATACGCCG GCGAAAGACG TCCTCGGAAA ATCGCCACCT 
GATCTGCAAA ACTTCAGCAA GAATTTACCA CAAAATGCCT TAAACTACTC CGAACTGAAT 
TACAACGAAA AACAACAAAT TAAAACATCG GAGCGGTTTG GCAAAAAGTC GCCGCAGTAC 
GTTAACGTGA CGGTGAGCGA GAAGAAAAAC ATTAATAATA AAGTGATCAT TTACGAAAAT 
GGATGTATTC CGAAAAACCC AAACCTCGAA TGCAACGAAC TGAATAATAA AAGCTCAAGC 
CACAAAAATA TTAACATGAA TCGAGCGCTC ACACCATCGC CCAACTATAA CAGGAATCCT 
TCGCCACATT TGAGGAGCAC AACGCCGAAT AATTGTAAGA ACGAAACCGA AATTACAGAA 
AACTGCAACG ACGCTGAACT GAAAAGGACA CAGGCGCAGC AAGAACGAAT CAAGGAGCAA 
GAAATCGGGA AAGCGGAACA GGCAAGACTT GAAGAAATTC TAAATATGTG CGTTGAATAC 
GAAAAGCAGG CACAATCGGA AAAAAATAAA CTCACGCCGA ATAGAATAAA AACAAACGGT 
TCGCTGCCGC GTGATAAGCG TCAGTATGGA CCCTTATCTC CTGTGTATAC CACTCCTCCA 
CCTTCGCCGA GAGACGTGGA AATTGTTCAG CATGAGAACA GGAGGAATAA TTCTTCGTAT 
GAGAATATTA GTATTAATAG GGATAATTCA CTGGAACGAA TTAATGCCAA TTATGATAAT 
GTTGAAATGA AGCAAGTTGA TGACTTTAGG ACTACGAAGT GTAACAGTCC GTATGAAAAT 
ATTGTTCCGG CGCATCAATC GCCAAGGACG AGGATTAAGA CTATCGCTTC GTCGAACCGA 
GACGGCAAAT CTAAGGACAC TGCCGAAATA ATCGAAAACA AATTCGCCAT TCTCGAAGCC 
GAGCAATACC TCCTCCAGAA TCTCACAATA AACGACGAAA AATCTCTCGA AACCGACAAA 
ATTCTCGCTG GCCGAGAAAA CACAAAATTC ATATTTCCCC TCGAAGAAAA ATCCTTCACC 
TCACTCCCTG CCAATTTAAA CGCCACGCCA TACAACAAAA AACCAAAACC AAACAACATA 
AACGACAAAC AAAAAATCGA AAAAATCCGA AAAGAACGCA AAGAAATTCT CTCGCAAATC 
TCGTCCATCA AGCGCCACAT CGCCGAAACG GAAATCCACG AAGAGGAACT GCAGCGCGAC 
ATTGAGCTCG AAAACGCCCT CATTTCCGGC GAACACAAAG CAAAATTGCT CGAACTGGAC 
AAATTGGAAC ACCGCAAGAA CAAACTCTTC AACTGTGCGC AAAAACTGGA AGAGAGTATG 
TCACACCGAC AGACGAAACA AGACGAAGAT CAGTTAAAAT GCAAAGAAAA GCTCGAACTA 
GCGCAGGAAA ATATGCTTCA AATCGAAGGC AAGTTGGCCA AGACGCCCAA AACCGACCCC 
AAGTACGAGA ACGTCTTCGA GGAGTATTTG AAAGCTCAAG AGATTTTGGA CAACGAGAAG 
AAAGCGTTCG AAGACTTGGA GTTCCACCAT CTGGAGGAGG AAGCCGATTG GTTGGCCAGT 
CGGGAAGAGG TCCAACGGGA AATTCTGGAT TTGTCCAAAA AGATTGACTC GGTTAAAGAC 
CAAATCCAGG AATTGGAACA ACAAAAGTAC AACATCTCGA AGAACAACAA CACTGAAGTG 
AAGATCATTC GCATGCGACG TGACGAATTC ATGCGCAAGT TGGAGAAGTG TTCGTTCGTT 
TTGCGGCGAA TTGACAACGA TTTGTCCGTT TTCGCCCATC AGGAGTCCGA CCAAGAGGAA 
TCAACTGACA GCGACAGCGA CAAATCGAAA GACCTGGACA AACATTTGTC CAATCTATCG 
TTGAATGTGA TCCATGACCT GAGCTGTTCG GTCATCGTGA GCAACACCAA GACCTCAATT 
GACCACGTGT ACAACATGTC GCAGTCATTT AACGAAAAAT TGTTGCAAGA AAAATCGGTT 
TTGGAGACCG GGATTGGCAC CAAATGTCCA AGTCAGGATG ACATTGACCG GATCAGTAAA 
GTAACATCAA GTGCCCCTAT TAGTATCAAT GAGGAGCAAG GATCTCTGGG AAGAAAGACA 
ATCGAATCGC TTAAAGATAT CGAAAGGAAG AGACATCTTC ATTTGTGCCA ACAAGGTTCG 
CAGGTTATCG AGCAGGAGAG ACAAAGAGTG CTGGCTTTGA AACAGAGGGT GCAGCATGAA 
GTGAGGATCA AGTGGGCGCA GAGCAGGCAG GATTGTGTGG TTCCTGAGTC AGGAGAGAAG 
AGAAACAGTG TTGAAATCGT AGGACGACAA CCCGAAAGGC CCGTTTCAAA ATCTCCTACG 
TCAAACGAAC AGATACGCTC CGGCTCAAGT GAGGCGGATT CGCCACGTCC AATGTCTGAA 
ATTAGTGAAA TAAGCACCGA TTTCGGAGAA ATCTCAAACA AGAAAAGAAC AAAACCCCAT 
GGCGACAAAC AACGACCCTT AACCCGATAT TTGCCCATCA GAGGCTCAGA CCTCGACCTA 
CGCCAACATA TAGAATCGGC AGGTCATCAA GTGGTGTTAT GTCCCCACGT CATCATCAAT 
TCGAGCAGCT GCAGAGGCTT TTTGCACAAA AAAGGGTCAA AATTGAACGG ATGGTCGAAA 
AGGTGGTTCG TTTTCGATCG AAATAAGCAT ACGCTGACTT ACTACAACGA CAAGAGTGAA 
AAGAAAGCTC GTGGAGGAGC CTATTTTCAA GCGATTGAAG AGGTCTATTT GGACCACCTC 
AACAGTGTTA AATCGCCGAA CCCCCAACTG ACCTTCATCG TGAAAACGCA CGAAAAGTCC 
TATTATCTAC TGGCGCCCTC GCCCGAAGCG ATGAGGATTT GGGTTGATGT TATCTTTACT 
GGTGCTGAAG GGTACCAAGA ATTTGGACAT GGTACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC660100 ( XM_008193048.2 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_008191270.1 Tribolium castaneum pleckstrin homology-like domain family B member 1 (LOC660100), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OTg03799
Clone ID Related Accession (Same CDS sequence)	XM_008193048.2 , XM_015978278.1
Accession Version	XM_008193048.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3609bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-20
Organism	Tribolium castaneum(red flour beetle)
Product	pleckstrin homology-like domain family B member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015450149.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 21, 2016 this sequence version replaced XM_008193048.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGCC TAAGTGGAAC CGAATCTTCG CCGGAGTTAT CAGACAGTGG AGGCAGAGCC 
CTGAAATTGC AAACCGACAC ACCACACTTG GTCAACATTG GGGGCGACAG ACTCAGCACT 
TCGGTGACGC TTCACCCCAT TCCTCAAGGT AGTCGGGTGA CGTTAGGATG CGGTCCAGGT 
GTTGATATTC GAGTGCAAGG GACTGGAGTC CTGCCTTTGC ATTGCCATAT CGAAAATAGT 
GAAGGTGTTG TTACCATTTA TCCGCTATCG GATAATCTCT CTGTTGATGG AGTGAAAATA 
TCCAGACCCA CTCGCCTATC TCAGGGTGTT ATGCTGACCA TCGGCCGATC AAACTACATG 
CGTTTTAACC ACCCGGCGGA AGCAAAAATA ATGAAATCAG TTCTCCCCAA CCCTCGCATC 
TCCATGGCTT CGATCACTTT CGAACCCGAC AATTCTTTTC AGTCTAAATA CAACAAAAAA 
CCACCAGCAG TGCCTCGAAA AAACTCGAAA GAATCCCTCA TTGAATCGGG TGTCGAAGAA 
CCGGTGCCTT CCAGTATTAT GACCAAAGTG TCAAAATTCG AGTATTTAGC CCAACAAAAT 
TTCAAAAAAA GCATATCACC GAAAGTGTTC TCTTCAAATC TCGTAACTGT AAATACGCCG 
GCGAAAGACG TCCTCGGAAA ATCGCCACCT GATCTGCAAA ACTTCAGCAA GAATTTACCA 
CAAAATGCCT TAAACTACTC CGAACTGAAT TACAACGAAA AACAACAAAT TAAAACATCG 
GAGCGGTTTG GCAAAAAGTC GCCGCAGTAC GTTAACGTGA CGGTGAGCGA GAAGAAAAAC 
ATTAATAATA AAGTGATCAT TTACGAAAAT GGATGTATTC CGAAAAACCC AAACCTCGAA 
TGCAACGAAC TGAATAATAA AAGCTCAAGC CACAAAAATA TTAACATGAA TCGAGCGCTC 
ACACCATCGC CCAACTATAA CAGGAATCCT TCGCCACATT TGAGGAGCAC AACGCCGAAT 
AATTGTAAGA ACGAAACCGA AATTACAGAA AACTGCAACG ACGCTGAACT GAAAAGGACA 
CAGGCGCAGC AAGAACGAAT CAAGGAGCAA GAAATCGGGA AAGCGGAACA GGCAAGACTT 
GAAGAAATTC TAAATATGTG CGTTGAATAC GAAAAGCAGG CACAATCGGA AAAAAATAAA 
CTCACGCCGA ATAGAATAAA AACAAACGGT TCGCTGCCGC GTGATAAGCG TCAGTATGGA 
CCCTTATCTC CTGTGTATAC CACTCCTCCA CCTTCGCCGA GAGACGTGGA AATTGTTCAG 
CATGAGAACA GGAGGAATAA TTCTTCGTAT GAGAATATTA GTATTAATAG GGATAATTCA 
CTGGAACGAA TTAATGCCAA TTATGATAAT GTTGAAATGA AGCAAGTTGA TGACTTTAGG 
ACTACGAAGT GTAACAGTCC GTATGAAAAT ATTGTTCCGG CGCATCAATC GCCAAGGACG 
AGGATTAAGA CTATCGCTTC GTCGAACCGA GACGGCAAAT CTAAGGACAC TGCCGAAATA 
ATCGAAAACA AATTCGCCAT TCTCGAAGCC GAGCAATACC TCCTCCAGAA TCTCACAATA 
AACGACGAAA AATCTCTCGA AACCGACAAA ATTCTCGCTG GCCGAGAAAA CACAAAATTC 
ATATTTCCCC TCGAAGAAAA ATCCTTCACC TCACTCCCTG CCAATTTAAA CGCCACGCCA 
TACAACAAAA AACCAAAACC AAACAACATA AACGACAAAC AAAAAATCGA AAAAATCCGA 
AAAGAACGCA AAGAAATTCT CTCGCAAATC TCGTCCATCA AGCGCCACAT CGCCGAAACG 
GAAATCCACG AAGAGGAACT GCAGCGCGAC ATTGAGCTCG AAAACGCCCT CATTTCCGGC 
GAACACAAAG CAAAATTGCT CGAACTGGAC AAATTGGAAC ACCGCAAGAA CAAACTCTTC 
AACTGTGCGC AAAAACTGGA AGAGAGTATG TCACACCGAC AGACGAAACA AGACGAAGAT 
CAGTTAAAAT GCAAAGAAAA GCTCGAACTA GCGCAGGAAA ATATGCTTCA AATCGAAGGC 
AAGTTGGCCA AGACGCCCAA AACCGACCCC AAGTACGAGA ACGTCTTCGA GGAGTATTTG 
AAAGCTCAAG AGATTTTGGA CAACGAGAAG AAAGCGTTCG AAGACTTGGA GTTCCACCAT 
CTGGAGGAGG AAGCCGATTG GTTGGCCAGT CGGGAAGAGG TCCAACGGGA AATTCTGGAT 
TTGTCCAAAA AGATTGACTC GGTTAAAGAC CAAATCCAGG AATTGGAACA ACAAAAGTAC 
AACATCTCGA AGAACAACAA CACTGAAGTG AAGATCATTC GCATGCGACG TGACGAATTC 
ATGCGCAAGT TGGAGAAGTG TTCGTTCGTT TTGCGGCGAA TTGACAACGA TTTGTCCGTT 
TTCGCCCATC AGGAGTCCGA CCAAGAGGAA TCAACTGACA GCGACAGCGA CAAATCGAAA 
GACCTGGACA AACATTTGTC CAATCTATCG TTGAATGTGA TCCATGACCT GAGCTGTTCG 
GTCATCGTGA GCAACACCAA GACCTCAATT GACCACGTGT ACAACATGTC GCAGTCATTT 
AACGAAAAAT TGTTGCAAGA AAAATCGGTT TTGGAGACCG GGATTGGCAC CAAATGTCCA 
AGTCAGGATG ACATTGACCG GATCAGTAAA GTAACATCAA GTGCCCCTAT TAGTATCAAT 
GAGGAGCAAG GATCTCTGGG AAGAAAGACA ATCGAATCGC TTAAAGATAT CGAAAGGAAG 
AGACATCTTC ATTTGTGCCA ACAAGGTTCG CAGGTTATCG AGCAGGAGAG ACAAAGAGTG 
CTGGCTTTGA AACAGAGGGT GCAGCATGAA GTGAGGATCA AGTGGGCGCA GAGCAGGCAG 
GATTGTGTGG TTCCTGAGTC AGGAGAGAAG AGAAACAGTG TTGAAATCGT AGGACGACAA 
CCCGAAAGGC CCGTTTCAAA ATCTCCTACG TCAAACGAAC AGATACGCTC CGGCTCAAGT 
GAGGCGGATT CGCCACGTCC AATGTCTGAA ATTAGTGAAA TAAGCACCGA TTTCGGAGAA 
ATCTCAAACA AGAAAAGAAC AAAACCCCAT GGCGACAAAC AACGACCCTT AACCCGATAT 
TTGCCCATCA GAGGCTCAGA CCTCGACCTA CGCCAACATA TAGAATCGGC AGGTCATCAA 
GTGGTGTTAT GTCCCCACGT CATCATCAAT TCGAGCAGCT GCAGAGGCTT TTTGCACAAA 
AAAGGGTCAA AATTGAACGG ATGGTCGAAA AGGTGGTTCG TTTTCGATCG AAATAAGCAT 
ACGCTGACTT ACTACAACGA CAAGAGTGAA AAGAAAGCTC GTGGAGGAGC CTATTTTCAA 
GCGATTGAAG AGGTCTATTT GGACCACCTC AACAGTGTTA AATCGCCGAA CCCCCAACTG 
ACCTTCATCG TGAAAACGCA CGAAAAGTCC TATTATCTAC TGGCGCCCTC GCCCGAAGCG 
ATGAGGATTT GGGTTGATGT TATCTTTACT GGTGCTGAAG GGTACCAAGA ATTTGGACAT 
GGTACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008191270.1
CDS	248..3856
Translation	MSGLSGTESSPELSDSGGRALKLQTDTPHLVNIGGDRLSTSVTLHPIPQGSRVTLGCGPGVDIRVQGTGVLPLHCHIENSEGVVTIYPLSDNLSVDGVKISRPTRLSQGVMLTIGRSNYMRFNHPAEAKIMKSVLPNPRISMASITFEPDNSFQSKYNKKPPAVPRKNSKESLIESGVEEPVPSSIMTKVSKFEYLAQQNFKKSISPKVFSSNLVTVNTPAKDVLGKSPPDLQNFSKNLPQNALNYSELNYNEKQQIKTSERFGKKSPQYVNVTVSEKKNINNKVIIYENGCIPKNPNLECNELNNKSSSHKNINMNRALTPSPNYNRNPSPHLRSTTPNNCKNETEITENCNDAELKRTQAQQERIKEQEIGKAEQARLEEILNMCVEYEKQAQSEKNKLTPNRIKTNGSLPRDKRQYGPLSPVYTTPPPSPRDVEIVQHENRRNNSSYENISINRDNSLERINANYDNVEMKQVDDFRTTKCNSPYENIVPAHQSPRTRIKTIASSNRDGKSKDTAEIIENKFAILEAEQYLLQNLTINDEKSLETDKILAGRENTKFIFPLEEKSFTSLPANLNATPYNKKPKPNNINDKQKIEKIRKERKEILSQISSIKRHIAETEIHEEELQRDIELENALISGEHKAKLLELDKLEHRKNKLFNCAQKLEESMSHRQTKQDEDQLKCKEKLELAQENMLQIEGKLAKTPKTDPKYENVFEEYLKAQEILDNEKKAFEDLEFHHLEEEADWLASREEVQREILDLSKKIDSVKDQIQELEQQKYNISKNNNTEVKIIRMRRDEFMRKLEKCSFVLRRIDNDLSVFAHQESDQEESTDSDSDKSKDLDKHLSNLSLNVIHDLSCSVIVSNTKTSIDHVYNMSQSFNEKLLQEKSVLETGIGTKCPSQDDIDRISKVTSSAPISINEEQGSLGRKTIESLKDIERKRHLHLCQQGSQVIEQERQRVLALKQRVQHEVRIKWAQSRQDCVVPESGEKRNSVEIVGRQPERPVSKSPTSNEQIRSGSSEADSPRPMSEISEISTDFGEISNKKRTKPHGDKQRPLTRYLPIRGSDLDLRQHIESAGHQVVLCPHVIINSSSCRGFLHKKGSKLNGWSKRWFVFDRNKHTLTYYNDKSEKKARGGAYFQAIEEVYLDHLNSVKSPNPQLTFIVKTHEKSYYLLAPSPEAMRIWVDVIFTGAEGYQEFGHGT

Target ORF information:

RefSeq Version	XM_008193048.2
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum pleckstrin homology-like domain family B member 1 (LOC660100), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008193048.2

ORF Insert Sequence:

ATGTCCGGCC TAAGTGGAAC CGAATCTTCG CCGGAGTTAT CAGACAGTGG AGGCAGAGCC 
CTGAAATTGC AAACCGACAC ACCACACTTG GTCAACATTG GGGGCGACAG ACTCAGCACT 
TCGGTGACGC TTCACCCCAT TCCTCAAGGT AGTCGGGTGA CGTTAGGATG CGGTCCAGGT 
GTTGATATTC GAGTGCAAGG GACTGGAGTC CTGCCTTTGC ATTGCCATAT CGAAAATAGT 
GAAGGTGTTG TTACCATTTA TCCGCTATCG GATAATCTCT CTGTTGATGG AGTGAAAATA 
TCCAGACCCA CTCGCCTATC TCAGGGTGTT ATGCTGACCA TCGGCCGATC AAACTACATG 
CGTTTTAACC ACCCGGCGGA AGCAAAAATA ATGAAATCAG TTCTCCCCAA CCCTCGCATC 
TCCATGGCTT CGATCACTTT CGAACCCGAC AATTCTTTTC AGTCTAAATA CAACAAAAAA 
CCACCAGCAG TGCCTCGAAA AAACTCGAAA GAATCCCTCA TTGAATCGGG TGTCGAAGAA 
CCGGTGCCTT CCAGTATTAT GACCAAAGTG TCAAAATTCG AGTATTTAGC CCAACAAAAT 
TTCAAAAAAA GCATATCACC GAAAGTGTTC TCTTCAAATC TCGTAACTGT AAATACGCCG 
GCGAAAGACG TCCTCGGAAA ATCGCCACCT GATCTGCAAA ACTTCAGCAA GAATTTACCA 
CAAAATGCCT TAAACTACTC CGAACTGAAT TACAACGAAA AACAACAAAT TAAAACATCG 
GAGCGGTTTG GCAAAAAGTC GCCGCAGTAC GTTAACGTGA CGGTGAGCGA GAAGAAAAAC 
ATTAATAATA AAGTGATCAT TTACGAAAAT GGATGTATTC CGAAAAACCC AAACCTCGAA 
TGCAACGAAC TGAATAATAA AAGCTCAAGC CACAAAAATA TTAACATGAA TCGAGCGCTC 
ACACCATCGC CCAACTATAA CAGGAATCCT TCGCCACATT TGAGGAGCAC AACGCCGAAT 
AATTGTAAGA ACGAAACCGA AATTACAGAA AACTGCAACG ACGCTGAACT GAAAAGGACA 
CAGGCGCAGC AAGAACGAAT CAAGGAGCAA GAAATCGGGA AAGCGGAACA GGCAAGACTT 
GAAGAAATTC TAAATATGTG CGTTGAATAC GAAAAGCAGG CACAATCGGA AAAAAATAAA 
CTCACGCCGA ATAGAATAAA AACAAACGGT TCGCTGCCGC GTGATAAGCG TCAGTATGGA 
CCCTTATCTC CTGTGTATAC CACTCCTCCA CCTTCGCCGA GAGACGTGGA AATTGTTCAG 
CATGAGAACA GGAGGAATAA TTCTTCGTAT GAGAATATTA GTATTAATAG GGATAATTCA 
CTGGAACGAA TTAATGCCAA TTATGATAAT GTTGAAATGA AGCAAGTTGA TGACTTTAGG 
ACTACGAAGT GTAACAGTCC GTATGAAAAT ATTGTTCCGG CGCATCAATC GCCAAGGACG 
AGGATTAAGA CTATCGCTTC GTCGAACCGA GACGGCAAAT CTAAGGACAC TGCCGAAATA 
ATCGAAAACA AATTCGCCAT TCTCGAAGCC GAGCAATACC TCCTCCAGAA TCTCACAATA 
AACGACGAAA AATCTCTCGA AACCGACAAA ATTCTCGCTG GCCGAGAAAA CACAAAATTC 
ATATTTCCCC TCGAAGAAAA ATCCTTCACC TCACTCCCTG CCAATTTAAA CGCCACGCCA 
TACAACAAAA AACCAAAACC AAACAACATA AACGACAAAC AAAAAATCGA AAAAATCCGA 
AAAGAACGCA AAGAAATTCT CTCGCAAATC TCGTCCATCA AGCGCCACAT CGCCGAAACG 
GAAATCCACG AAGAGGAACT GCAGCGCGAC ATTGAGCTCG AAAACGCCCT CATTTCCGGC 
GAACACAAAG CAAAATTGCT CGAACTGGAC AAATTGGAAC ACCGCAAGAA CAAACTCTTC 
AACTGTGCGC AAAAACTGGA AGAGAGTATG TCACACCGAC AGACGAAACA AGACGAAGAT 
CAGTTAAAAT GCAAAGAAAA GCTCGAACTA GCGCAGGAAA ATATGCTTCA AATCGAAGGC 
AAGTTGGCCA AGACGCCCAA AACCGACCCC AAGTACGAGA ACGTCTTCGA GGAGTATTTG 
AAAGCTCAAG AGATTTTGGA CAACGAGAAG AAAGCGTTCG AAGACTTGGA GTTCCACCAT 
CTGGAGGAGG AAGCCGATTG GTTGGCCAGT CGGGAAGAGG TCCAACGGGA AATTCTGGAT 
TTGTCCAAAA AGATTGACTC GGTTAAAGAC CAAATCCAGG AATTGGAACA ACAAAAGTAC 
AACATCTCGA AGAACAACAA CACTGAAGTG AAGATCATTC GCATGCGACG TGACGAATTC 
ATGCGCAAGT TGGAGAAGTG TTCGTTCGTT TTGCGGCGAA TTGACAACGA TTTGTCCGTT 
TTCGCCCATC AGGAGTCCGA CCAAGAGGAA TCAACTGACA GCGACAGCGA CAAATCGAAA 
GACCTGGACA AACATTTGTC CAATCTATCG TTGAATGTGA TCCATGACCT GAGCTGTTCG 
GTCATCGTGA GCAACACCAA GACCTCAATT GACCACGTGT ACAACATGTC GCAGTCATTT 
AACGAAAAAT TGTTGCAAGA AAAATCGGTT TTGGAGACCG GGATTGGCAC CAAATGTCCA 
AGTCAGGATG ACATTGACCG GATCAGTAAA GTAACATCAA GTGCCCCTAT TAGTATCAAT 
GAGGAGCAAG GATCTCTGGG AAGAAAGACA ATCGAATCGC TTAAAGATAT CGAAAGGAAG 
AGACATCTTC ATTTGTGCCA ACAAGGTTCG CAGGTTATCG AGCAGGAGAG ACAAAGAGTG 
CTGGCTTTGA AACAGAGGGT GCAGCATGAA GTGAGGATCA AGTGGGCGCA GAGCAGGCAG 
GATTGTGTGG TTCCTGAGTC AGGAGAGAAG AGAAACAGTG TTGAAATCGT AGGACGACAA 
CCCGAAAGGC CCGTTTCAAA ATCTCCTACG TCAAACGAAC AGATACGCTC CGGCTCAAGT 
GAGGCGGATT CGCCACGTCC AATGTCTGAA ATTAGTGAAA TAAGCACCGA TTTCGGAGAA 
ATCTCAAACA AGAAAAGAAC AAAACCCCAT GGCGACAAAC AACGACCCTT AACCCGATAT 
TTGCCCATCA GAGGCTCAGA CCTCGACCTA CGCCAACATA TAGAATCGGC AGGTCATCAA 
GTGGTGTTAT GTCCCCACGT CATCATCAAT TCGAGCAGCT GCAGAGGCTT TTTGCACAAA 
AAAGGGTCAA AATTGAACGG ATGGTCGAAA AGGTGGTTCG TTTTCGATCG AAATAAGCAT 
ACGCTGACTT ACTACAACGA CAAGAGTGAA AAGAAAGCTC GTGGAGGAGC CTATTTTCAA 
GCGATTGAAG AGGTCTATTT GGACCACCTC AACAGTGTTA AATCGCCGAA CCCCCAACTG 
ACCTTCATCG TGAAAACGCA CGAAAAGTCC TATTATCTAC TGGCGCCCTC GCCCGAAGCG 
ATGAGGATTT GGGTTGATGT TATCTTTACT GGTGCTGAAG GGTACCAAGA ATTTGGACAT 
GGTACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC660100 ( XM_015978278.1 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_015833764.1 Tribolium castaneum pleckstrin homology-like domain family B member 1 (LOC660100), transcript variant X2, mRNA.

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CloneID	OTg03799
Clone ID Related Accession (Same CDS sequence)	XM_008193048.2 , XM_015978278.1
Accession Version	XM_015978278.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3609bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-20
Organism	Tribolium castaneum(red flour beetle)
Product	pleckstrin homology-like domain family B member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015450149.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGCC TAAGTGGAAC CGAATCTTCG CCGGAGTTAT CAGACAGTGG AGGCAGAGCC 
CTGAAATTGC AAACCGACAC ACCACACTTG GTCAACATTG GGGGCGACAG ACTCAGCACT 
TCGGTGACGC TTCACCCCAT TCCTCAAGGT AGTCGGGTGA CGTTAGGATG CGGTCCAGGT 
GTTGATATTC GAGTGCAAGG GACTGGAGTC CTGCCTTTGC ATTGCCATAT CGAAAATAGT 
GAAGGTGTTG TTACCATTTA TCCGCTATCG GATAATCTCT CTGTTGATGG AGTGAAAATA 
TCCAGACCCA CTCGCCTATC TCAGGGTGTT ATGCTGACCA TCGGCCGATC AAACTACATG 
CGTTTTAACC ACCCGGCGGA AGCAAAAATA ATGAAATCAG TTCTCCCCAA CCCTCGCATC 
TCCATGGCTT CGATCACTTT CGAACCCGAC AATTCTTTTC AGTCTAAATA CAACAAAAAA 
CCACCAGCAG TGCCTCGAAA AAACTCGAAA GAATCCCTCA TTGAATCGGG TGTCGAAGAA 
CCGGTGCCTT CCAGTATTAT GACCAAAGTG TCAAAATTCG AGTATTTAGC CCAACAAAAT 
TTCAAAAAAA GCATATCACC GAAAGTGTTC TCTTCAAATC TCGTAACTGT AAATACGCCG 
GCGAAAGACG TCCTCGGAAA ATCGCCACCT GATCTGCAAA ACTTCAGCAA GAATTTACCA 
CAAAATGCCT TAAACTACTC CGAACTGAAT TACAACGAAA AACAACAAAT TAAAACATCG 
GAGCGGTTTG GCAAAAAGTC GCCGCAGTAC GTTAACGTGA CGGTGAGCGA GAAGAAAAAC 
ATTAATAATA AAGTGATCAT TTACGAAAAT GGATGTATTC CGAAAAACCC AAACCTCGAA 
TGCAACGAAC TGAATAATAA AAGCTCAAGC CACAAAAATA TTAACATGAA TCGAGCGCTC 
ACACCATCGC CCAACTATAA CAGGAATCCT TCGCCACATT TGAGGAGCAC AACGCCGAAT 
AATTGTAAGA ACGAAACCGA AATTACAGAA AACTGCAACG ACGCTGAACT GAAAAGGACA 
CAGGCGCAGC AAGAACGAAT CAAGGAGCAA GAAATCGGGA AAGCGGAACA GGCAAGACTT 
GAAGAAATTC TAAATATGTG CGTTGAATAC GAAAAGCAGG CACAATCGGA AAAAAATAAA 
CTCACGCCGA ATAGAATAAA AACAAACGGT TCGCTGCCGC GTGATAAGCG TCAGTATGGA 
CCCTTATCTC CTGTGTATAC CACTCCTCCA CCTTCGCCGA GAGACGTGGA AATTGTTCAG 
CATGAGAACA GGAGGAATAA TTCTTCGTAT GAGAATATTA GTATTAATAG GGATAATTCA 
CTGGAACGAA TTAATGCCAA TTATGATAAT GTTGAAATGA AGCAAGTTGA TGACTTTAGG 
ACTACGAAGT GTAACAGTCC GTATGAAAAT ATTGTTCCGG CGCATCAATC GCCAAGGACG 
AGGATTAAGA CTATCGCTTC GTCGAACCGA GACGGCAAAT CTAAGGACAC TGCCGAAATA 
ATCGAAAACA AATTCGCCAT TCTCGAAGCC GAGCAATACC TCCTCCAGAA TCTCACAATA 
AACGACGAAA AATCTCTCGA AACCGACAAA ATTCTCGCTG GCCGAGAAAA CACAAAATTC 
ATATTTCCCC TCGAAGAAAA ATCCTTCACC TCACTCCCTG CCAATTTAAA CGCCACGCCA 
TACAACAAAA AACCAAAACC AAACAACATA AACGACAAAC AAAAAATCGA AAAAATCCGA 
AAAGAACGCA AAGAAATTCT CTCGCAAATC TCGTCCATCA AGCGCCACAT CGCCGAAACG 
GAAATCCACG AAGAGGAACT GCAGCGCGAC ATTGAGCTCG AAAACGCCCT CATTTCCGGC 
GAACACAAAG CAAAATTGCT CGAACTGGAC AAATTGGAAC ACCGCAAGAA CAAACTCTTC 
AACTGTGCGC AAAAACTGGA AGAGAGTATG TCACACCGAC AGACGAAACA AGACGAAGAT 
CAGTTAAAAT GCAAAGAAAA GCTCGAACTA GCGCAGGAAA ATATGCTTCA AATCGAAGGC 
AAGTTGGCCA AGACGCCCAA AACCGACCCC AAGTACGAGA ACGTCTTCGA GGAGTATTTG 
AAAGCTCAAG AGATTTTGGA CAACGAGAAG AAAGCGTTCG AAGACTTGGA GTTCCACCAT 
CTGGAGGAGG AAGCCGATTG GTTGGCCAGT CGGGAAGAGG TCCAACGGGA AATTCTGGAT 
TTGTCCAAAA AGATTGACTC GGTTAAAGAC CAAATCCAGG AATTGGAACA ACAAAAGTAC 
AACATCTCGA AGAACAACAA CACTGAAGTG AAGATCATTC GCATGCGACG TGACGAATTC 
ATGCGCAAGT TGGAGAAGTG TTCGTTCGTT TTGCGGCGAA TTGACAACGA TTTGTCCGTT 
TTCGCCCATC AGGAGTCCGA CCAAGAGGAA TCAACTGACA GCGACAGCGA CAAATCGAAA 
GACCTGGACA AACATTTGTC CAATCTATCG TTGAATGTGA TCCATGACCT GAGCTGTTCG 
GTCATCGTGA GCAACACCAA GACCTCAATT GACCACGTGT ACAACATGTC GCAGTCATTT 
AACGAAAAAT TGTTGCAAGA AAAATCGGTT TTGGAGACCG GGATTGGCAC CAAATGTCCA 
AGTCAGGATG ACATTGACCG GATCAGTAAA GTAACATCAA GTGCCCCTAT TAGTATCAAT 
GAGGAGCAAG GATCTCTGGG AAGAAAGACA ATCGAATCGC TTAAAGATAT CGAAAGGAAG 
AGACATCTTC ATTTGTGCCA ACAAGGTTCG CAGGTTATCG AGCAGGAGAG ACAAAGAGTG 
CTGGCTTTGA AACAGAGGGT GCAGCATGAA GTGAGGATCA AGTGGGCGCA GAGCAGGCAG 
GATTGTGTGG TTCCTGAGTC AGGAGAGAAG AGAAACAGTG TTGAAATCGT AGGACGACAA 
CCCGAAAGGC CCGTTTCAAA ATCTCCTACG TCAAACGAAC AGATACGCTC CGGCTCAAGT 
GAGGCGGATT CGCCACGTCC AATGTCTGAA ATTAGTGAAA TAAGCACCGA TTTCGGAGAA 
ATCTCAAACA AGAAAAGAAC AAAACCCCAT GGCGACAAAC AACGACCCTT AACCCGATAT 
TTGCCCATCA GAGGCTCAGA CCTCGACCTA CGCCAACATA TAGAATCGGC AGGTCATCAA 
GTGGTGTTAT GTCCCCACGT CATCATCAAT TCGAGCAGCT GCAGAGGCTT TTTGCACAAA 
AAAGGGTCAA AATTGAACGG ATGGTCGAAA AGGTGGTTCG TTTTCGATCG AAATAAGCAT 
ACGCTGACTT ACTACAACGA CAAGAGTGAA AAGAAAGCTC GTGGAGGAGC CTATTTTCAA 
GCGATTGAAG AGGTCTATTT GGACCACCTC AACAGTGTTA AATCGCCGAA CCCCCAACTG 
ACCTTCATCG TGAAAACGCA CGAAAAGTCC TATTATCTAC TGGCGCCCTC GCCCGAAGCG 
ATGAGGATTT GGGTTGATGT TATCTTTACT GGTGCTGAAG GGTACCAAGA ATTTGGACAT 
GGTACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015833764.1
CDS	19..3627
Translation	MSGLSGTESSPELSDSGGRALKLQTDTPHLVNIGGDRLSTSVTLHPIPQGSRVTLGCGPGVDIRVQGTGVLPLHCHIENSEGVVTIYPLSDNLSVDGVKISRPTRLSQGVMLTIGRSNYMRFNHPAEAKIMKSVLPNPRISMASITFEPDNSFQSKYNKKPPAVPRKNSKESLIESGVEEPVPSSIMTKVSKFEYLAQQNFKKSISPKVFSSNLVTVNTPAKDVLGKSPPDLQNFSKNLPQNALNYSELNYNEKQQIKTSERFGKKSPQYVNVTVSEKKNINNKVIIYENGCIPKNPNLECNELNNKSSSHKNINMNRALTPSPNYNRNPSPHLRSTTPNNCKNETEITENCNDAELKRTQAQQERIKEQEIGKAEQARLEEILNMCVEYEKQAQSEKNKLTPNRIKTNGSLPRDKRQYGPLSPVYTTPPPSPRDVEIVQHENRRNNSSYENISINRDNSLERINANYDNVEMKQVDDFRTTKCNSPYENIVPAHQSPRTRIKTIASSNRDGKSKDTAEIIENKFAILEAEQYLLQNLTINDEKSLETDKILAGRENTKFIFPLEEKSFTSLPANLNATPYNKKPKPNNINDKQKIEKIRKERKEILSQISSIKRHIAETEIHEEELQRDIELENALISGEHKAKLLELDKLEHRKNKLFNCAQKLEESMSHRQTKQDEDQLKCKEKLELAQENMLQIEGKLAKTPKTDPKYENVFEEYLKAQEILDNEKKAFEDLEFHHLEEEADWLASREEVQREILDLSKKIDSVKDQIQELEQQKYNISKNNNTEVKIIRMRRDEFMRKLEKCSFVLRRIDNDLSVFAHQESDQEESTDSDSDKSKDLDKHLSNLSLNVIHDLSCSVIVSNTKTSIDHVYNMSQSFNEKLLQEKSVLETGIGTKCPSQDDIDRISKVTSSAPISINEEQGSLGRKTIESLKDIERKRHLHLCQQGSQVIEQERQRVLALKQRVQHEVRIKWAQSRQDCVVPESGEKRNSVEIVGRQPERPVSKSPTSNEQIRSGSSEADSPRPMSEISEISTDFGEISNKKRTKPHGDKQRPLTRYLPIRGSDLDLRQHIESAGHQVVLCPHVIINSSSCRGFLHKKGSKLNGWSKRWFVFDRNKHTLTYYNDKSEKKARGGAYFQAIEEVYLDHLNSVKSPNPQLTFIVKTHEKSYYLLAPSPEAMRIWVDVIFTGAEGYQEFGHGT

Target ORF information:

RefSeq Version	XM_015978278.1
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum pleckstrin homology-like domain family B member 1 (LOC660100), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015978278.1

ORF Insert Sequence:

ATGTCCGGCC TAAGTGGAAC CGAATCTTCG CCGGAGTTAT CAGACAGTGG AGGCAGAGCC 
CTGAAATTGC AAACCGACAC ACCACACTTG GTCAACATTG GGGGCGACAG ACTCAGCACT 
TCGGTGACGC TTCACCCCAT TCCTCAAGGT AGTCGGGTGA CGTTAGGATG CGGTCCAGGT 
GTTGATATTC GAGTGCAAGG GACTGGAGTC CTGCCTTTGC ATTGCCATAT CGAAAATAGT 
GAAGGTGTTG TTACCATTTA TCCGCTATCG GATAATCTCT CTGTTGATGG AGTGAAAATA 
TCCAGACCCA CTCGCCTATC TCAGGGTGTT ATGCTGACCA TCGGCCGATC AAACTACATG 
CGTTTTAACC ACCCGGCGGA AGCAAAAATA ATGAAATCAG TTCTCCCCAA CCCTCGCATC 
TCCATGGCTT CGATCACTTT CGAACCCGAC AATTCTTTTC AGTCTAAATA CAACAAAAAA 
CCACCAGCAG TGCCTCGAAA AAACTCGAAA GAATCCCTCA TTGAATCGGG TGTCGAAGAA 
CCGGTGCCTT CCAGTATTAT GACCAAAGTG TCAAAATTCG AGTATTTAGC CCAACAAAAT 
TTCAAAAAAA GCATATCACC GAAAGTGTTC TCTTCAAATC TCGTAACTGT AAATACGCCG 
GCGAAAGACG TCCTCGGAAA ATCGCCACCT GATCTGCAAA ACTTCAGCAA GAATTTACCA 
CAAAATGCCT TAAACTACTC CGAACTGAAT TACAACGAAA AACAACAAAT TAAAACATCG 
GAGCGGTTTG GCAAAAAGTC GCCGCAGTAC GTTAACGTGA CGGTGAGCGA GAAGAAAAAC 
ATTAATAATA AAGTGATCAT TTACGAAAAT GGATGTATTC CGAAAAACCC AAACCTCGAA 
TGCAACGAAC TGAATAATAA AAGCTCAAGC CACAAAAATA TTAACATGAA TCGAGCGCTC 
ACACCATCGC CCAACTATAA CAGGAATCCT TCGCCACATT TGAGGAGCAC AACGCCGAAT 
AATTGTAAGA ACGAAACCGA AATTACAGAA AACTGCAACG ACGCTGAACT GAAAAGGACA 
CAGGCGCAGC AAGAACGAAT CAAGGAGCAA GAAATCGGGA AAGCGGAACA GGCAAGACTT 
GAAGAAATTC TAAATATGTG CGTTGAATAC GAAAAGCAGG CACAATCGGA AAAAAATAAA 
CTCACGCCGA ATAGAATAAA AACAAACGGT TCGCTGCCGC GTGATAAGCG TCAGTATGGA 
CCCTTATCTC CTGTGTATAC CACTCCTCCA CCTTCGCCGA GAGACGTGGA AATTGTTCAG 
CATGAGAACA GGAGGAATAA TTCTTCGTAT GAGAATATTA GTATTAATAG GGATAATTCA 
CTGGAACGAA TTAATGCCAA TTATGATAAT GTTGAAATGA AGCAAGTTGA TGACTTTAGG 
ACTACGAAGT GTAACAGTCC GTATGAAAAT ATTGTTCCGG CGCATCAATC GCCAAGGACG 
AGGATTAAGA CTATCGCTTC GTCGAACCGA GACGGCAAAT CTAAGGACAC TGCCGAAATA 
ATCGAAAACA AATTCGCCAT TCTCGAAGCC GAGCAATACC TCCTCCAGAA TCTCACAATA 
AACGACGAAA AATCTCTCGA AACCGACAAA ATTCTCGCTG GCCGAGAAAA CACAAAATTC 
ATATTTCCCC TCGAAGAAAA ATCCTTCACC TCACTCCCTG CCAATTTAAA CGCCACGCCA 
TACAACAAAA AACCAAAACC AAACAACATA AACGACAAAC AAAAAATCGA AAAAATCCGA 
AAAGAACGCA AAGAAATTCT CTCGCAAATC TCGTCCATCA AGCGCCACAT CGCCGAAACG 
GAAATCCACG AAGAGGAACT GCAGCGCGAC ATTGAGCTCG AAAACGCCCT CATTTCCGGC 
GAACACAAAG CAAAATTGCT CGAACTGGAC AAATTGGAAC ACCGCAAGAA CAAACTCTTC 
AACTGTGCGC AAAAACTGGA AGAGAGTATG TCACACCGAC AGACGAAACA AGACGAAGAT 
CAGTTAAAAT GCAAAGAAAA GCTCGAACTA GCGCAGGAAA ATATGCTTCA AATCGAAGGC 
AAGTTGGCCA AGACGCCCAA AACCGACCCC AAGTACGAGA ACGTCTTCGA GGAGTATTTG 
AAAGCTCAAG AGATTTTGGA CAACGAGAAG AAAGCGTTCG AAGACTTGGA GTTCCACCAT 
CTGGAGGAGG AAGCCGATTG GTTGGCCAGT CGGGAAGAGG TCCAACGGGA AATTCTGGAT 
TTGTCCAAAA AGATTGACTC GGTTAAAGAC CAAATCCAGG AATTGGAACA ACAAAAGTAC 
AACATCTCGA AGAACAACAA CACTGAAGTG AAGATCATTC GCATGCGACG TGACGAATTC 
ATGCGCAAGT TGGAGAAGTG TTCGTTCGTT TTGCGGCGAA TTGACAACGA TTTGTCCGTT 
TTCGCCCATC AGGAGTCCGA CCAAGAGGAA TCAACTGACA GCGACAGCGA CAAATCGAAA 
GACCTGGACA AACATTTGTC CAATCTATCG TTGAATGTGA TCCATGACCT GAGCTGTTCG 
GTCATCGTGA GCAACACCAA GACCTCAATT GACCACGTGT ACAACATGTC GCAGTCATTT 
AACGAAAAAT TGTTGCAAGA AAAATCGGTT TTGGAGACCG GGATTGGCAC CAAATGTCCA 
AGTCAGGATG ACATTGACCG GATCAGTAAA GTAACATCAA GTGCCCCTAT TAGTATCAAT 
GAGGAGCAAG GATCTCTGGG AAGAAAGACA ATCGAATCGC TTAAAGATAT CGAAAGGAAG 
AGACATCTTC ATTTGTGCCA ACAAGGTTCG CAGGTTATCG AGCAGGAGAG ACAAAGAGTG 
CTGGCTTTGA AACAGAGGGT GCAGCATGAA GTGAGGATCA AGTGGGCGCA GAGCAGGCAG 
GATTGTGTGG TTCCTGAGTC AGGAGAGAAG AGAAACAGTG TTGAAATCGT AGGACGACAA 
CCCGAAAGGC CCGTTTCAAA ATCTCCTACG TCAAACGAAC AGATACGCTC CGGCTCAAGT 
GAGGCGGATT CGCCACGTCC AATGTCTGAA ATTAGTGAAA TAAGCACCGA TTTCGGAGAA 
ATCTCAAACA AGAAAAGAAC AAAACCCCAT GGCGACAAAC AACGACCCTT AACCCGATAT 
TTGCCCATCA GAGGCTCAGA CCTCGACCTA CGCCAACATA TAGAATCGGC AGGTCATCAA 
GTGGTGTTAT GTCCCCACGT CATCATCAAT TCGAGCAGCT GCAGAGGCTT TTTGCACAAA 
AAAGGGTCAA AATTGAACGG ATGGTCGAAA AGGTGGTTCG TTTTCGATCG AAATAAGCAT 
ACGCTGACTT ACTACAACGA CAAGAGTGAA AAGAAAGCTC GTGGAGGAGC CTATTTTCAA 
GCGATTGAAG AGGTCTATTT GGACCACCTC AACAGTGTTA AATCGCCGAA CCCCCAACTG 
ACCTTCATCG TGAAAACGCA CGAAAAGTCC TATTATCTAC TGGCGCCCTC GCCCGAAGCG 
ATGAGGATTT GGGTTGATGT TATCTTTACT GGTGCTGAAG GGTACCAAGA ATTTGGACAT 
GGTACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC660100 ( XM_966360.3 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_971453.1 Tribolium castaneum pleckstrin homology-like domain family B member 1 (LOC660100), transcript variant X3, mRNA.

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CloneID	OTg03800
Clone ID Related Accession (Same CDS sequence)	XM_966360.3
Accession Version	XM_966360.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-20
Organism	Tribolium castaneum(red flour beetle)
Product	pleckstrin homology-like domain family B member 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015450149.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 21, 2016 this sequence version replaced XM_966360.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGCC TAAGTGGAAC CGAATCTTCG CCGGAGTTAT CAGACAGTGG AGGCAGAGCC 
CTGAAATTGC AAACCGACAC ACCACACTTG GTCAACATTG GGGGCGACAG ACTCAGCACT 
TCGGTGACGC TTCACCCCAT TCCTCAAGGT CGGGTGACGT TAGGATGCGG TCCAGGTGTT 
GATATTCGAG TGCAAGGGAC TGGAGTCCTG CCTTTGCATT GCCATATCGA AAATAGTGAA 
GGTGTTGTTA CCATTTATCC GCTATCGGAT AATCTCTCTG TTGATGGAGT GAAAATATCC 
AGACCCACTC GCCTATCTCA GGGTGTTATG CTGACCATCG GCCGATCAAA CTACATGCGT 
TTTAACCACC CGGCGGAAGC AAAAATAATG AAATCAGTTC TCCCCAACCC TCGCATCTCC 
ATGGCTTCGA TCACTTTCGA ACCCGACAAT TCTTTTCAGT CTAAATACAA CAAAAAACCA 
CCAGCAGTGC CTCGAAAAAA CTCGAAAGAA TCCCTCATTG AATCGGGTGT CGAAGAACCG 
GTGCCTTCCA GTATTATGAC CAAAGTGTCA AAATTCGAGT ATTTAGCCCA ACAAAATTTC 
AAAAAAAGCA TATCACCGAA AGTGTTCTCT TCAAATCTCG TAACTGTAAA TACGCCGGCG 
AAAGACGTCC TCGGAAAATC GCCACCTGAT CTGCAAAACT TCAGCAAGAA TTTACCACAA 
AATGCCTTAA ACTACTCCGA ACTGAATTAC AACGAAAAAC AACAAATTAA AACATCGGAG 
CGGTTTGGCA AAAAGTCGCC GCAGTACGTT AACGTGACGG TGAGCGAGAA GAAAAACATT 
AATAATAAAG TGATCATTTA CGAAAATGGA TGTATTCCGA AAAACCCAAA CCTCGAATGC 
AACGAACTGA ATAATAAAAG CTCAAGCCAC AAAAATATTA ACATGAATCG AGCGCTCACA 
CCATCGCCCA ACTATAACAG GAATCCTTCG CCACATTTGA GGAGCACAAC GCCGAATAAT 
TGTAAGAACG AAACCGAAAT TACAGAAAAC TGCAACGACG CTGAACTGAA AAGGACACAG 
GCGCAGCAAG AACGAATCAA GGAGCAAGAA ATCGGGAAAG CGGAACAGGC AAGACTTGAA 
GAAATTCTAA ATATGTGCGT TGAATACGAA AAGCAGGCAC AATCGGAAAA AAATAAACTC 
ACGCCGAATA GAATAAAAAC AAACGGTTCG CTGCCGCGTG ATAAGCGTCA GTATGGACCC 
TTATCTCCTG TGTATACCAC TCCTCCACCT TCGCCGAGAG ACGTGGAAAT TGTTCAGCAT 
GAGAACAGGA GGAATAATTC TTCGTATGAG AATATTAGTA TTAATAGGGA TAATTCACTG 
GAACGAATTA ATGCCAATTA TGATAATGTT GAAATGAAGC AAGTTGATGA CTTTAGGACT 
ACGAAGTGTA ACAGTCCGTA TGAAAATATT GTTCCGGCGC ATCAATCGCC AAGGACGAGG 
ATTAAGACTA TCGCTTCGTC GAACCGAGAC GGCAAATCTA AGGACACTGC CGAAATAATC 
GAAAACAAAT TCGCCATTCT CGAAGCCGAG CAATACCTCC TCCAGAATCT CACAATAAAC 
GACGAAAAAT CTCTCGAAAC CGACAAAATT CTCGCTGGCC GAGAAAACAC AAAATTCATA 
TTTCCCCTCG AAGAAAAATC CTTCACCTCA CTCCCTGCCA ATTTAAACGC CACGCCATAC 
AACAAAAAAC CAAAACCAAA CAACATAAAC GACAAACAAA AAATCGAAAA AATCCGAAAA 
GAACGCAAAG AAATTCTCTC GCAAATCTCG TCCATCAAGC GCCACATCGC CGAAACGGAA 
ATCCACGAAG AGGAACTGCA GCGCGACATT GAGCTCGAAA ACGCCCTCAT TTCCGGCGAA 
CACAAAGCAA AATTGCTCGA ACTGGACAAA TTGGAACACC GCAAGAACAA ACTCTTCAAC 
TGTGCGCAAA AACTGGAAGA GAGTATGTCA CACCGACAGA CGAAACAAGA CGAAGATCAG 
TTAAAATGCA AAGAAAAGCT CGAACTAGCG CAGGAAAATA TGCTTCAAAT CGAAGGCAAG 
TTGGCCAAGA CGCCCAAAAC CGACCCCAAG TACGAGAACG TCTTCGAGGA GTATTTGAAA 
GCTCAAGAGA TTTTGGACAA CGAGAAGAAA GCGTTCGAAG ACTTGGAGTT CCACCATCTG 
GAGGAGGAAG CCGATTGGTT GGCCAGTCGG GAAGAGGTCC AACGGGAAAT TCTGGATTTG 
TCCAAAAAGA TTGACTCGGT TAAAGACCAA ATCCAGGAAT TGGAACAACA AAAGTACAAC 
ATCTCGAAGA ACAACAACAC TGAAGTGAAG ATCATTCGCA TGCGACGTGA CGAATTCATG 
CGCAAGTTGG AGAAGTGTTC GTTCGTTTTG CGGCGAATTG ACAACGATTT GTCCGTTTTC 
GCCCATCAGG AGTCCGACCA AGAGGAATCA ACTGACAGCG ACAGCGACAA ATCGAAAGAC 
CTGGACAAAC ATTTGTCCAA TCTATCGTTG AATGTGATCC ATGACCTGAG CTGTTCGGTC 
ATCGTGAGCA ACACCAAGAC CTCAATTGAC CACGTGTACA ACATGTCGCA GTCATTTAAC 
GAAAAATTGT TGCAAGAAAA ATCGGTTTTG GAGACCGGGA TTGGCACCAA ATGTCCAAGT 
CAGGATGACA TTGACCGGAT CAGTAAAGTA ACATCAAGTG CCCCTATTAG TATCAATGAG 
GAGCAAGGAT CTCTGGGAAG AAAGACAATC GAATCGCTTA AAGATATCGA AAGGAAGAGA 
CATCTTCATT TGTGCCAACA AGGTTCGCAG GTTATCGAGC AGGAGAGACA AAGAGTGCTG 
GCTTTGAAAC AGAGGGTGCA GCATGAAGTG AGGATCAAGT GGGCGCAGAG CAGGCAGGAT 
TGTGTGGTTC CTGAGTCAGG AGAGAAGAGA AACAGTGTTG AAATCGTAGG ACGACAACCC 
GAAAGGCCCG TTTCAAAATC TCCTACGTCA AACGAACAGA TACGCTCCGG CTCAAGTGAG 
GCGGATTCGC CACGTCCAAT GTCTGAAATT AGTGAAATAA GCACCGATTT CGGAGAAATC 
TCAAACAAGA AAAGAACAAA ACCCCATGGC GACAAACAAC GACCCTTAAC CCGATATTTG 
CCCATCAGAG GCTCAGACCT CGACCTACGC CAACATATAG AATCGGCAGG TCATCAAGTG 
GTGTTATGTC CCCACGTCAT CATCAATTCG AGCAGCTGCA GAGGCTTTTT GCACAAAAAA 
GGGTCAAAAT TGAACGGATG GTCGAAAAGG TGGTTCGTTT TCGATCGAAA TAAGCATACG 
CTGACTTACT ACAACGACAA GAGTGAAAAG AAAGCTCGTG GAGGAGCCTA TTTTCAAGCG 
ATTGAAGAGG TCTATTTGGA CCACCTCAAC AGTGTTAAAT CGCCGAACCC CCAACTGACC 
TTCATCGTGA AAACGCACGA AAAGTCCTAT TATCTACTGG CGCCCTCGCC CGAAGCGATG 
AGGATTTGGG TTGATGTTAT CTTTACTGGT GCTGAAGGGT ACCAAGAATT TGGACATGGT 
ACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_971453.1
CDS	248..3853
Translation	MSGLSGTESSPELSDSGGRALKLQTDTPHLVNIGGDRLSTSVTLHPIPQGRVTLGCGPGVDIRVQGTGVLPLHCHIENSEGVVTIYPLSDNLSVDGVKISRPTRLSQGVMLTIGRSNYMRFNHPAEAKIMKSVLPNPRISMASITFEPDNSFQSKYNKKPPAVPRKNSKESLIESGVEEPVPSSIMTKVSKFEYLAQQNFKKSISPKVFSSNLVTVNTPAKDVLGKSPPDLQNFSKNLPQNALNYSELNYNEKQQIKTSERFGKKSPQYVNVTVSEKKNINNKVIIYENGCIPKNPNLECNELNNKSSSHKNINMNRALTPSPNYNRNPSPHLRSTTPNNCKNETEITENCNDAELKRTQAQQERIKEQEIGKAEQARLEEILNMCVEYEKQAQSEKNKLTPNRIKTNGSLPRDKRQYGPLSPVYTTPPPSPRDVEIVQHENRRNNSSYENISINRDNSLERINANYDNVEMKQVDDFRTTKCNSPYENIVPAHQSPRTRIKTIASSNRDGKSKDTAEIIENKFAILEAEQYLLQNLTINDEKSLETDKILAGRENTKFIFPLEEKSFTSLPANLNATPYNKKPKPNNINDKQKIEKIRKERKEILSQISSIKRHIAETEIHEEELQRDIELENALISGEHKAKLLELDKLEHRKNKLFNCAQKLEESMSHRQTKQDEDQLKCKEKLELAQENMLQIEGKLAKTPKTDPKYENVFEEYLKAQEILDNEKKAFEDLEFHHLEEEADWLASREEVQREILDLSKKIDSVKDQIQELEQQKYNISKNNNTEVKIIRMRRDEFMRKLEKCSFVLRRIDNDLSVFAHQESDQEESTDSDSDKSKDLDKHLSNLSLNVIHDLSCSVIVSNTKTSIDHVYNMSQSFNEKLLQEKSVLETGIGTKCPSQDDIDRISKVTSSAPISINEEQGSLGRKTIESLKDIERKRHLHLCQQGSQVIEQERQRVLALKQRVQHEVRIKWAQSRQDCVVPESGEKRNSVEIVGRQPERPVSKSPTSNEQIRSGSSEADSPRPMSEISEISTDFGEISNKKRTKPHGDKQRPLTRYLPIRGSDLDLRQHIESAGHQVVLCPHVIINSSSCRGFLHKKGSKLNGWSKRWFVFDRNKHTLTYYNDKSEKKARGGAYFQAIEEVYLDHLNSVKSPNPQLTFIVKTHEKSYYLLAPSPEAMRIWVDVIFTGAEGYQEFGHGT

Target ORF information:

RefSeq Version	XM_966360.3
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum pleckstrin homology-like domain family B member 1 (LOC660100), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_966360.3

ORF Insert Sequence:

ATGTCCGGCC TAAGTGGAAC CGAATCTTCG CCGGAGTTAT CAGACAGTGG AGGCAGAGCC 
CTGAAATTGC AAACCGACAC ACCACACTTG GTCAACATTG GGGGCGACAG ACTCAGCACT 
TCGGTGACGC TTCACCCCAT TCCTCAAGGT CGGGTGACGT TAGGATGCGG TCCAGGTGTT 
GATATTCGAG TGCAAGGGAC TGGAGTCCTG CCTTTGCATT GCCATATCGA AAATAGTGAA 
GGTGTTGTTA CCATTTATCC GCTATCGGAT AATCTCTCTG TTGATGGAGT GAAAATATCC 
AGACCCACTC GCCTATCTCA GGGTGTTATG CTGACCATCG GCCGATCAAA CTACATGCGT 
TTTAACCACC CGGCGGAAGC AAAAATAATG AAATCAGTTC TCCCCAACCC TCGCATCTCC 
ATGGCTTCGA TCACTTTCGA ACCCGACAAT TCTTTTCAGT CTAAATACAA CAAAAAACCA 
CCAGCAGTGC CTCGAAAAAA CTCGAAAGAA TCCCTCATTG AATCGGGTGT CGAAGAACCG 
GTGCCTTCCA GTATTATGAC CAAAGTGTCA AAATTCGAGT ATTTAGCCCA ACAAAATTTC 
AAAAAAAGCA TATCACCGAA AGTGTTCTCT TCAAATCTCG TAACTGTAAA TACGCCGGCG 
AAAGACGTCC TCGGAAAATC GCCACCTGAT CTGCAAAACT TCAGCAAGAA TTTACCACAA 
AATGCCTTAA ACTACTCCGA ACTGAATTAC AACGAAAAAC AACAAATTAA AACATCGGAG 
CGGTTTGGCA AAAAGTCGCC GCAGTACGTT AACGTGACGG TGAGCGAGAA GAAAAACATT 
AATAATAAAG TGATCATTTA CGAAAATGGA TGTATTCCGA AAAACCCAAA CCTCGAATGC 
AACGAACTGA ATAATAAAAG CTCAAGCCAC AAAAATATTA ACATGAATCG AGCGCTCACA 
CCATCGCCCA ACTATAACAG GAATCCTTCG CCACATTTGA GGAGCACAAC GCCGAATAAT 
TGTAAGAACG AAACCGAAAT TACAGAAAAC TGCAACGACG CTGAACTGAA AAGGACACAG 
GCGCAGCAAG AACGAATCAA GGAGCAAGAA ATCGGGAAAG CGGAACAGGC AAGACTTGAA 
GAAATTCTAA ATATGTGCGT TGAATACGAA AAGCAGGCAC AATCGGAAAA AAATAAACTC 
ACGCCGAATA GAATAAAAAC AAACGGTTCG CTGCCGCGTG ATAAGCGTCA GTATGGACCC 
TTATCTCCTG TGTATACCAC TCCTCCACCT TCGCCGAGAG ACGTGGAAAT TGTTCAGCAT 
GAGAACAGGA GGAATAATTC TTCGTATGAG AATATTAGTA TTAATAGGGA TAATTCACTG 
GAACGAATTA ATGCCAATTA TGATAATGTT GAAATGAAGC AAGTTGATGA CTTTAGGACT 
ACGAAGTGTA ACAGTCCGTA TGAAAATATT GTTCCGGCGC ATCAATCGCC AAGGACGAGG 
ATTAAGACTA TCGCTTCGTC GAACCGAGAC GGCAAATCTA AGGACACTGC CGAAATAATC 
GAAAACAAAT TCGCCATTCT CGAAGCCGAG CAATACCTCC TCCAGAATCT CACAATAAAC 
GACGAAAAAT CTCTCGAAAC CGACAAAATT CTCGCTGGCC GAGAAAACAC AAAATTCATA 
TTTCCCCTCG AAGAAAAATC CTTCACCTCA CTCCCTGCCA ATTTAAACGC CACGCCATAC 
AACAAAAAAC CAAAACCAAA CAACATAAAC GACAAACAAA AAATCGAAAA AATCCGAAAA 
GAACGCAAAG AAATTCTCTC GCAAATCTCG TCCATCAAGC GCCACATCGC CGAAACGGAA 
ATCCACGAAG AGGAACTGCA GCGCGACATT GAGCTCGAAA ACGCCCTCAT TTCCGGCGAA 
CACAAAGCAA AATTGCTCGA ACTGGACAAA TTGGAACACC GCAAGAACAA ACTCTTCAAC 
TGTGCGCAAA AACTGGAAGA GAGTATGTCA CACCGACAGA CGAAACAAGA CGAAGATCAG 
TTAAAATGCA AAGAAAAGCT CGAACTAGCG CAGGAAAATA TGCTTCAAAT CGAAGGCAAG 
TTGGCCAAGA CGCCCAAAAC CGACCCCAAG TACGAGAACG TCTTCGAGGA GTATTTGAAA 
GCTCAAGAGA TTTTGGACAA CGAGAAGAAA GCGTTCGAAG ACTTGGAGTT CCACCATCTG 
GAGGAGGAAG CCGATTGGTT GGCCAGTCGG GAAGAGGTCC AACGGGAAAT TCTGGATTTG 
TCCAAAAAGA TTGACTCGGT TAAAGACCAA ATCCAGGAAT TGGAACAACA AAAGTACAAC 
ATCTCGAAGA ACAACAACAC TGAAGTGAAG ATCATTCGCA TGCGACGTGA CGAATTCATG 
CGCAAGTTGG AGAAGTGTTC GTTCGTTTTG CGGCGAATTG ACAACGATTT GTCCGTTTTC 
GCCCATCAGG AGTCCGACCA AGAGGAATCA ACTGACAGCG ACAGCGACAA ATCGAAAGAC 
CTGGACAAAC ATTTGTCCAA TCTATCGTTG AATGTGATCC ATGACCTGAG CTGTTCGGTC 
ATCGTGAGCA ACACCAAGAC CTCAATTGAC CACGTGTACA ACATGTCGCA GTCATTTAAC 
GAAAAATTGT TGCAAGAAAA ATCGGTTTTG GAGACCGGGA TTGGCACCAA ATGTCCAAGT 
CAGGATGACA TTGACCGGAT CAGTAAAGTA ACATCAAGTG CCCCTATTAG TATCAATGAG 
GAGCAAGGAT CTCTGGGAAG AAAGACAATC GAATCGCTTA AAGATATCGA AAGGAAGAGA 
CATCTTCATT TGTGCCAACA AGGTTCGCAG GTTATCGAGC AGGAGAGACA AAGAGTGCTG 
GCTTTGAAAC AGAGGGTGCA GCATGAAGTG AGGATCAAGT GGGCGCAGAG CAGGCAGGAT 
TGTGTGGTTC CTGAGTCAGG AGAGAAGAGA AACAGTGTTG AAATCGTAGG ACGACAACCC 
GAAAGGCCCG TTTCAAAATC TCCTACGTCA AACGAACAGA TACGCTCCGG CTCAAGTGAG 
GCGGATTCGC CACGTCCAAT GTCTGAAATT AGTGAAATAA GCACCGATTT CGGAGAAATC 
TCAAACAAGA AAAGAACAAA ACCCCATGGC GACAAACAAC GACCCTTAAC CCGATATTTG 
CCCATCAGAG GCTCAGACCT CGACCTACGC CAACATATAG AATCGGCAGG TCATCAAGTG 
GTGTTATGTC CCCACGTCAT CATCAATTCG AGCAGCTGCA GAGGCTTTTT GCACAAAAAA 
GGGTCAAAAT TGAACGGATG GTCGAAAAGG TGGTTCGTTT TCGATCGAAA TAAGCATACG 
CTGACTTACT ACAACGACAA GAGTGAAAAG AAAGCTCGTG GAGGAGCCTA TTTTCAAGCG 
ATTGAAGAGG TCTATTTGGA CCACCTCAAC AGTGTTAAAT CGCCGAACCC CCAACTGACC 
TTCATCGTGA AAACGCACGA AAAGTCCTAT TATCTACTGG CGCCCTCGCC CGAAGCGATG 
AGGATTTGGG TTGATGTTAT CTTTACTGGT GCTGAAGGGT ACCAAGAATT TGGACATGGT 
ACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC660100 cDNA ORF clone, Tribolium castaneum(red flour beetle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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