wwp1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Gene Symbol	wwp1
Entrez Gene ID	100038255
Full Name	WW domain containing E3 ubiquitin protein ligase 1
Synonyms	aip5,hsdrp1,tiul1
General protein information	Preferred Names WW domain containing E3 ubiquitin protein ligase 1 Names NEDD4-like E3 ubiquitin-protein ligase WWP1 WW domain-containing protein 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001097375.1	NP_001090844.1	NEDD4-like E3 ubiquitin-protein ligase WWP1
XM_012964151.2	XP_012819605.1	NEDD4-like E3 ubiquitin-protein ligase WWP1 isoform X1
XM_012964151.3	XP_012819605.2	NEDD4-like E3 ubiquitin-protein ligase WWP1 isoform X1
XM_012964150.2	XP_012819604.1	NEDD4-like E3 ubiquitin-protein ligase WWP1 isoform X1
XM_018094505.1	XP_017949994.1	NEDD4-like E3 ubiquitin-protein ligase WWP1 isoform X2
XM_012964150.3	XP_012819604.2	NEDD4-like E3 ubiquitin-protein ligase WWP1 isoform X1
XM_012964152.3	XP_012819606.2	NEDD4-like E3 ubiquitin-protein ligase WWP1 isoform X1
XM_012964152.2	XP_012819606.1	NEDD4-like E3 ubiquitin-protein ligase WWP1 isoform X1

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009074	XP_319824.3
Homo sapiens (human)	WWP1	NP_008944.1
Pan troglodytes (chimpanzee)	WWP1	XP_519843.2
Drosophila melanogaster (fruit fly)	Su(dx)	NP_722754.1
	WWP1	XP_001083173.1
Bos taurus (cattle)	WWP1	NP_001032540.1
Mus musculus (house mouse)	Wwp1	NP_796301.2
Rattus norvegicus (Norway rat)	Wwp1	NP_001019928.1
Caenorhabditis elegans (roundworm)	wwp-1	NP_740775.1
Xenopus tropicalis (tropical clawed frog)	wwp1	NP_001090844.1
Canis lupus familiaris (dog)	WWP1	XP_003640065.1
Gallus gallus (chicken)	WWP1	NP_001012572.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following wwp1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the wwp1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa24470	NM_001097375.1 Latest version!	Xenopus tropicalis WW domain containing E3 ubiquitin protein ligase 1 (wwp1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa30166	XM_012964150.2 Latest version!	Xenopus tropicalis WW domain containing E3 ubiquitin protein ligase 1 (wwp1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa30166	XM_012964151.2 Latest version!	Xenopus tropicalis WW domain containing E3 ubiquitin protein ligase 1 (wwp1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa30166	XM_012964152.2 Latest version!	Xenopus tropicalis WW domain containing E3 ubiquitin protein ligase 1 (wwp1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa24470	XM_012964150.3 Latest version!	Xenopus tropicalis WW domain containing E3 ubiquitin protein ligase 1 (wwp1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa24470	XM_012964151.3 Latest version!	Xenopus tropicalis WW domain containing E3 ubiquitin protein ligase 1 (wwp1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa24470	XM_012964152.3 Latest version!	Xenopus tropicalis WW domain containing E3 ubiquitin protein ligase 1 (wwp1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa44834	XM_018094505.1 Latest version!	Xenopus tropicalis WW domain containing E3 ubiquitin protein ligase 1 (wwp1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

wwp1 ( NM_001097375.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001090844.1 Xenopus tropicalis WW domain containing E3 ubiquitin protein ligase 1 (wwp1), mRNA.

Price & Availability↑

CloneID	OXa24470
Clone ID Related Accession (Same CDS sequence)	NM_001097375.1 , XM_012964151.3 , XM_012964150.3 , XM_012964152.3
Accession Version	NM_001097375.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	NEDD4-like E3 ubiquitin-protein ligase WWP1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC121961.1. ##Evidence-Data-START## Transcript exon combination :: BC121961.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028290, SAMD00028291 [ECO:0000348] ##Evidence-Data-END##

ATGGCCACAG CCTCGCCACG GCCGGGGAAT CGAAACAGTC TTGAAGATTT ACAGCTGCAC 
GTGACAGTGT CCTGTGCCAA ACTGAAGCGG AAGAAGAACT GGTTTGGAAC GACTGTATAT 
ACAGAATTAG CTGTTGATGG TGAAACAAAG AAGACTGCAA AATCAAGCAG CTCGAGTAAC 
CCCAAATGGG AAGAACGGTT AACTGTGAGT GTGACCCCAC AAACTACACT TGAATTCAAG 
GTATGGAGCC ACCATTCGTG GAAAGCGGAT GCGTTGCTTG GCAAAACCGC TGTCGACTTA 
AAGCAAGCTC TGGAGACCCA TAACCGGAAG TTGGAAAGAG TGAAAGAACA ACTGAAACTT 
TCATTGGACG GCAAACATGG CACAGTACAG ACTGGCGAGC TGACAGTCAT TTTAGATGGA 
TTAAATGTGG AGCCTGAATC ACTTGCTACG TTTAACTCAA CAGCCACTCC AGCCAGAGAA 
ATCCAGCAGA ATGGGGATGC CGTCCGTGAG AATGGAGGAG AAACCTCTTT GGAAGCAAGA 
GTTGGAGCCA CTTGCGACAT GCCCAATGGG ACAGCTAGTG AAACCACATC CGAGCAGAAT 
GCCTTTAATA CACAAATAGC AAATATAGAT AGCACTCATT CCCCTTCTCG TGTTGCAGCC 
AGACCCAAAA ATACACCAGC ACCAAATCCA CTGACTGGCG ATAGAACTGT TAATGGCACA 
AGTGCTACAT CTGAAGCAAA TAGCACACCT TCCCATGAAG CTTTACCATC TCCTGCCGGA 
GCCCCCTCAT CAGATAGCAT AGAGATGATG GACAGTACAT CAGATGGGCC AGTATCAATA 
CCTTTAGATA GCGGCACGTC TGCAGAGAGC AGTTCTGCTG GGGCTTCATC AGAACCAGTA 
AGGGCTGCAG ACTCTGGGCC ATCACCTGCT GCTGGCAGCA ATAGCCCTGA TGCTGCAAAA 
CCAAGGCAGG CGTCATCTAC AGGTTCTGGG GATCCTGTCA GACAGCAGCC AAGCTCCGCT 
GCAGAGCCTT TGCCATCGGG ATGGGAACAG AGGAAAGACC CCCATGGTAG AACCTACTAC 
GTTGATCACA ACACAAGGAC TACAACATGG GAACGCCCAC AGCCTTTGCC TCCTGGGTGG 
GAGAGAAGGG TCGATGATCG TGGGAGAGTA TATTATGTTG ACCATAACAC AAGAACCACA 
ACATGGCAGA GGCCTACTAT GGAGTCTGTA CGAAACTTTG AACAGTGGCA GTCTCAGCGC 
AATCAACTCC AGGGAGCTAT GCAGCAGTTT AACCAGCGAT ACCTCTATTC GGCATCAATG 
TTATCCGCTG AAAATGACCC GTTAGGACCA CTTCCGCCAG GATGGGAGAG AAGAGTGGAT 
TCCACTGATC GTGTCTATTT TGTGAACCAC AATACAAAAA CAACACAGTG GGAGGACCCT 
CGAACTCAAG GTTTACAGAA CGAAGATCCA TTGCCAGAGG GCTGGGAAAT CCGCTACACA 
AGAGAAGGAG TAAAGTACTT TGTAGATCAC AATACAAAGA CGACTACTTT CAGCGACCCA 
AGAACTGGGA AGTCAGCAGT AAGCAAAGGG CCACAGATTG CCTATGAACG TAGTTTCCGC 
TGGAAGCTTG CTCATTTTCG TTATTTATGC CAGTCCAATG CACTGCCCAG CCATGTGAAG 
ATAACGGTGT CCAGGCAGAC GCTCTTTGAA GACTCTTTCC AGCAGATTAT GGCATTAAAA 
CCATATGATC TGAGAAGGCG ATTGTATGTC ATGTTCAGAG GGGAAGAAGG TTTGGACTAT 
GGAGGCTTAG CCAGAGAGTG GTTTTTCCTG CTTTCCCATG AGGTACTGAA CCCCATGTAC 
TGCCTGTTCG AGTATGCAGG CAAGAGCAAT TACTGCTTGC AGATCAACCC TGCCTCCACC 
ATTAATCCTG ACCACCTCTC CTATTTCTGC TTTATTGGCC GCTTTATTGC AATGGCGCTG 
TTCCATGGGA AGTTTATTGA TACTGGCTTC TCTCTTCCAT TCTACAAACG AATGCTCAGC 
AAGAAACTTA CCATTAAAGA TCTGGAATCG ATTGATCCCG AGTTCTACAA CTCTTTGATA 
TGGATACGGG ACAATAACAT TGAAGAATGT AACCTGGAAA TGTACTTTTC TGTGGACATG 
GAAATATTGG GGAAAGTCAC CTCACATGAT CTGAAGCCTG AAGGTTCCAA CATCCTTGTG 
ACTGAGGAGA ACAAGGAAGA ATATATTGGT CTCATGGCAG AATGGAGATT CTCACGTGGA 
GTTGAGGAAC AAACCAAAGC ATTTCTTGAT GGCTTTAATG CAGTGGTCCC ACTTCAGTGG 
CTACAGTACT TTGATGAAAA AGAGTTAGAG GTTATGCTGT GTGGCATGCA GGAGGTGGAT 
TTATCAGACT GGCAGCGGAA TACAGTCTAT CGGCATTACA CAAGGAATAG CAAGCAGATC 
ATATGGTTCT GGCAGTTTGT GAAGGAAATG GACAATGAGG TGCGGTTGCG GCTGCTGCAG 
TTTGTCACTG GTACCTGCAG GCTGCCGCTG GGAGGATTCG CAGAGCTCAT GGGAAGCAAC 
GGTCCACAGA AATTCTGCAT TGAGAAGGTT GGAAAAGAAA CATGGCTCCC TCGAAGTCAT 
ACATGTTTCA ATCGACTGGA CCTGCCGCCA TATAAAAGCT ATGAACAACT GAAGGAGAAG 
CTGCTTTTTG CAATTGAAGA AACGGAAGGA TTTGGCCAAG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001090844.1
CDS	95..2839
Translation	MATASPRPGNRNSLEDLQLHVTVSCAKLKRKKNWFGTTVYTELAVDGETKKTAKSSSSSNPKWEERLTVSVTPQTTLEFKVWSHHSWKADALLGKTAVDLKQALETHNRKLERVKEQLKLSLDGKHGTVQTGELTVILDGLNVEPESLATFNSTATPAREIQQNGDAVRENGGETSLEARVGATCDMPNGTASETTSEQNAFNTQIANIDSTHSPSRVAARPKNTPAPNPLTGDRTVNGTSATSEANSTPSHEALPSPAGAPSSDSIEMMDSTSDGPVSIPLDSGTSAESSSAGASSEPVRAADSGPSPAAGSNSPDAAKPRQASSTGSGDPVRQQPSSAAEPLPSGWEQRKDPHGRTYYVDHNTRTTTWERPQPLPPGWERRVDDRGRVYYVDHNTRTTTWQRPTMESVRNFEQWQSQRNQLQGAMQQFNQRYLYSASMLSAENDPLGPLPPGWERRVDSTDRVYFVNHNTKTTQWEDPRTQGLQNEDPLPEGWEIRYTREGVKYFVDHNTKTTTFSDPRTGKSAVSKGPQIAYERSFRWKLAHFRYLCQSNALPSHVKITVSRQTLFEDSFQQIMALKPYDLRRRLYVMFRGEEGLDYGGLAREWFFLLSHEVLNPMYCLFEYAGKSNYCLQINPASTINPDHLSYFCFIGRFIAMALFHGKFIDTGFSLPFYKRMLSKKLTIKDLESIDPEFYNSLIWIRDNNIEECNLEMYFSVDMEILGKVTSHDLKPEGSNILVTEENKEEYIGLMAEWRFSRGVEEQTKAFLDGFNAVVPLQWLQYFDEKELEVMLCGMQEVDLSDWQRNTVYRHYTRNSKQIIWFWQFVKEMDNEVRLRLLQFVTGTCRLPLGGFAELMGSNGPQKFCIEKVGKETWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	NM_001097375.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis WW domain containing E3 ubiquitin protein ligase 1 (wwp1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001097375.1

ORF Insert Sequence:

ATGGCCACAG CCTCGCCACG GCCGGGGAAT CGAAACAGTC TTGAAGATTT ACAGCTGCAC 
GTGACAGTGT CCTGTGCCAA ACTGAAGCGG AAGAAGAACT GGTTTGGAAC GACTGTATAT 
ACAGAATTAG CTGTTGATGG TGAAACAAAG AAGACTGCAA AATCAAGCAG CTCGAGTAAC 
CCCAAATGGG AAGAACGGTT AACTGTGAGT GTGACCCCAC AAACTACACT TGAATTCAAG 
GTATGGAGCC ACCATTCGTG GAAAGCGGAT GCGTTGCTTG GCAAAACCGC TGTCGACTTA 
AAGCAAGCTC TGGAGACCCA TAACCGGAAG TTGGAAAGAG TGAAAGAACA ACTGAAACTT 
TCATTGGACG GCAAACATGG CACAGTACAG ACTGGCGAGC TGACAGTCAT TTTAGATGGA 
TTAAATGTGG AGCCTGAATC ACTTGCTACG TTTAACTCAA CAGCCACTCC AGCCAGAGAA 
ATCCAGCAGA ATGGGGATGC CGTCCGTGAG AATGGAGGAG AAACCTCTTT GGAAGCAAGA 
GTTGGAGCCA CTTGCGACAT GCCCAATGGG ACAGCTAGTG AAACCACATC CGAGCAGAAT 
GCCTTTAATA CACAAATAGC AAATATAGAT AGCACTCATT CCCCTTCTCG TGTTGCAGCC 
AGACCCAAAA ATACACCAGC ACCAAATCCA CTGACTGGCG ATAGAACTGT TAATGGCACA 
AGTGCTACAT CTGAAGCAAA TAGCACACCT TCCCATGAAG CTTTACCATC TCCTGCCGGA 
GCCCCCTCAT CAGATAGCAT AGAGATGATG GACAGTACAT CAGATGGGCC AGTATCAATA 
CCTTTAGATA GCGGCACGTC TGCAGAGAGC AGTTCTGCTG GGGCTTCATC AGAACCAGTA 
AGGGCTGCAG ACTCTGGGCC ATCACCTGCT GCTGGCAGCA ATAGCCCTGA TGCTGCAAAA 
CCAAGGCAGG CGTCATCTAC AGGTTCTGGG GATCCTGTCA GACAGCAGCC AAGCTCCGCT 
GCAGAGCCTT TGCCATCGGG ATGGGAACAG AGGAAAGACC CCCATGGTAG AACCTACTAC 
GTTGATCACA ACACAAGGAC TACAACATGG GAACGCCCAC AGCCTTTGCC TCCTGGGTGG 
GAGAGAAGGG TCGATGATCG TGGGAGAGTA TATTATGTTG ACCATAACAC AAGAACCACA 
ACATGGCAGA GGCCTACTAT GGAGTCTGTA CGAAACTTTG AACAGTGGCA GTCTCAGCGC 
AATCAACTCC AGGGAGCTAT GCAGCAGTTT AACCAGCGAT ACCTCTATTC GGCATCAATG 
TTATCCGCTG AAAATGACCC GTTAGGACCA CTTCCGCCAG GATGGGAGAG AAGAGTGGAT 
TCCACTGATC GTGTCTATTT TGTGAACCAC AATACAAAAA CAACACAGTG GGAGGACCCT 
CGAACTCAAG GTTTACAGAA CGAAGATCCA TTGCCAGAGG GCTGGGAAAT CCGCTACACA 
AGAGAAGGAG TAAAGTACTT TGTAGATCAC AATACAAAGA CGACTACTTT CAGCGACCCA 
AGAACTGGGA AGTCAGCAGT AAGCAAAGGG CCACAGATTG CCTATGAACG TAGTTTCCGC 
TGGAAGCTTG CTCATTTTCG TTATTTATGC CAGTCCAATG CACTGCCCAG CCATGTGAAG 
ATAACGGTGT CCAGGCAGAC GCTCTTTGAA GACTCTTTCC AGCAGATTAT GGCATTAAAA 
CCATATGATC TGAGAAGGCG ATTGTATGTC ATGTTCAGAG GGGAAGAAGG TTTGGACTAT 
GGAGGCTTAG CCAGAGAGTG GTTTTTCCTG CTTTCCCATG AGGTACTGAA CCCCATGTAC 
TGCCTGTTCG AGTATGCAGG CAAGAGCAAT TACTGCTTGC AGATCAACCC TGCCTCCACC 
ATTAATCCTG ACCACCTCTC CTATTTCTGC TTTATTGGCC GCTTTATTGC AATGGCGCTG 
TTCCATGGGA AGTTTATTGA TACTGGCTTC TCTCTTCCAT TCTACAAACG AATGCTCAGC 
AAGAAACTTA CCATTAAAGA TCTGGAATCG ATTGATCCCG AGTTCTACAA CTCTTTGATA 
TGGATACGGG ACAATAACAT TGAAGAATGT AACCTGGAAA TGTACTTTTC TGTGGACATG 
GAAATATTGG GGAAAGTCAC CTCACATGAT CTGAAGCCTG AAGGTTCCAA CATCCTTGTG 
ACTGAGGAGA ACAAGGAAGA ATATATTGGT CTCATGGCAG AATGGAGATT CTCACGTGGA 
GTTGAGGAAC AAACCAAAGC ATTTCTTGAT GGCTTTAATG CAGTGGTCCC ACTTCAGTGG 
CTACAGTACT TTGATGAAAA AGAGTTAGAG GTTATGCTGT GTGGCATGCA GGAGGTGGAT 
TTATCAGACT GGCAGCGGAA TACAGTCTAT CGGCATTACA CAAGGAATAG CAAGCAGATC 
ATATGGTTCT GGCAGTTTGT GAAGGAAATG GACAATGAGG TGCGGTTGCG GCTGCTGCAG 
TTTGTCACTG GTACCTGCAG GCTGCCGCTG GGAGGATTCG CAGAGCTCAT GGGAAGCAAC 
GGTCCACAGA AATTCTGCAT TGAGAAGGTT GGAAAAGAAA CATGGCTCCC TCGAAGTCAT 
ACATGTTTCA ATCGACTGGA CCTGCCGCCA TATAAAAGCT ATGAACAACT GAAGGAGAAG 
CTGCTTTTTG CAATTGAAGA AACGGAAGGA TTTGGCCAAG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wwp1 ( XM_012964151.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819605.1 Xenopus tropicalis WW domain containing E3 ubiquitin protein ligase 1 (wwp1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa30166
Clone ID Related Accession (Same CDS sequence)	XM_012964151.2 , XM_012964150.2 , XM_012964152.2
Accession Version	XM_012964151.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	NEDD4-like E3 ubiquitin-protein ligase WWP1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012964151.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG CCTCGCCACG GCCGGGGAAT CGAAACAGTC TTGAAGATTT ACAGCTGCAC 
GTGACAGTGT CCTGTGCCAA ACTGAAGCGG AAGAAGAACT GGTTTGGAAC GACTGTATAT 
ACAGAATTAG CTGTTGATGG TGAAACAAAG AAGACTGCAA AATCAAGCAG CTCGAGTAAC 
CCCAAATGGG AAGAACGGTT AACTGTGAGT GTGACCCCAC AAACTACACT TGAATTCAAG 
GTATGGAGCC ACCATTCGTG GAAAGCGGAT GCGTTGCTTG GCAAAACCGC TGTCGACTTA 
AAGCAAGCTC TGGAGACCCA TAACCGGAAG TTGGAAAGAG TGAAAGAACA ACTGAAACTT 
TCATTGGACG GCAAACATGG CACAGTACAG ACTGGCGAGC TGACAGTCAT TTTAGATGGA 
TTAAATGTGG AGCCTGAATC ACTTGCTACG TTTAACTCAA CAGCCACTCC AGCCAGAGAA 
ATCCAGCAGA ATGGGGATGC CGTCCGTGAG AATGGAGGAG AAACCTCTTT GGAAGCAAGA 
GTTGGAGCCA CTTGCGACAT GCCCAATGGG ACAGCTAGTG AAACCACATC CGAGCAGAAT 
GCCTTTAATA CACAAATAGC AAATATAGAT GGCACTCATT CCCCTTCTCG TGTTGCAGCC 
AGACCCAAAA ATACACCAGC ACCAAATCCA CTGACTGGCG ATAGAACTGT TAATGGCACA 
AGTGCTACAT CTGAAGCAAA TAGCACACCT TCCCATGAAG CTTTACCATC TCCTGCCGGA 
GCCCCCTCAT CAGATAGCAT AGAGATGATG GACAGTACAT CAGATGGGCC AGTATCAATA 
CCTTTAGATA GCGGCACGTC TGCAGAGAGC AGTTCTGCTG GGGCTTCATC AGAACCAGTA 
AGGGCTGCAG ACTCTGGGCC ATCACCTGCT GCTGGCAGCA ATAGCCCTGA TGCTGCAAAA 
CCAAGGCAGG CGTCATCTAC AGGTTCTGGG GATCCTGTCA GACAGCAGCC AAGCTCCGCT 
GCAGAGCCTT TGCCATCGGG ATGGGAACAG AGGAAAGACC CCCATGGTAG AACCTACTAC 
GTTGATCACA ACACAAGGAC TACAACATGG GAACGCCCAC AGCCTTTGCC TCCTGGGTGG 
GAGAGAAGGG TCGATGATCG TGGGAGAGTA TATTATGTTG ACCATAACAC AAGAACCACA 
ACATGGCAGA GGCCTACTAT GGAGTCTGTA CGAAACTTTG AACAGTGGCA GTCTCAGCGC 
AATCAACTCC AGGGAGCTAT GCAGCAGTTT AACCAGCGAT ACCTCTATTC GGCATCAATG 
TTATCCGCTG AAAATGACCC GTTAGGACCA CTTCCGCCAG GATGGGAGAG AAGAGTGGAT 
TCCACTGATC GTGTCTATTT TGTGAACCAC AATACAAAAA CAACACAGTG GGAGGACCCT 
CGAACTCAAG GTTTACAGAA CGAAGATCCA TTGCCAGAGG GCTGGGAAAT CCGCTACACA 
AGAGAAGGAG TAAAGTACTT TGTAGATCAC AATACAAAGA CGACTACTTT CAGCGACCCA 
AGAACTGGGA AGTCAGCAGT AAGCAAAGGG CCACAGATTG CCTATGAACG TAGTTTCCGC 
TGGAAGCTTG CTCATTTTCG TTATTTATGC CAGTCCAATG CACTGCCCAG CCATGTGAAG 
ATAACGGTGT CCAGGCAGAC GCTCTTTGAA GACTCTTTCC AGCAGATTAT GGCATTAAAA 
CCATATGATC TGAGAAGGCG ATTGTATGTC ATGTTCAGAG GGGAAGAAGG TCTGGACTAT 
GGAGGCTTAG CCAGAGAGTG GTTTTTCCTG CTTTCCCATG AGGTACTGAA CCCCATGTAC 
TGCCTGTTCG AGTATGCAGG CAAGAGCAAT TACTGCTTGC AGATCAACCC TGCCTCCACC 
ATTAATCCTG ACCACCTCTC CTATTTCTGC TTTATTGGCC GCTTTATTGC AATGGCGCTG 
TTCCATGGGA AGTTTATTGA TACTGGCTTC TCTCTTCCAT TCTACAAACG AATGCTCAGC 
AAGAAACTTA CCATTAAAGA TCTGGAATCG ATTGATCCCG AGTTCTACAA CTCTTTGATA 
TGGATACGGG ACAATAACAT TGAAGAATGT AACCTGGAAA TGTACTTTTC TGTGGACATG 
GAAATATTGG GGAAAGTCAC CTCACATGAT CTGAAGCCTG AAGGTTCCAA CATCCTTGTG 
ACTGAGGAGA ACAAGGAAGA ATATATTGGT CTCATGGCAG AATGGAGATT CTCACGTGGA 
GTTGAAGAAC AAACCAAAGC ATTTCTTGAT GGCTTTAATG CAGTGGTCCC ACTTCAGTGG 
CTACAGTACT TTGATGAAAA AGAGTTAGAG GTTATGCTGT GTGGCATGCA GGAGGTGGAT 
TTATCAGACT GGCAGCGGAA TACAGTCTAT CGGCATTACA CAAGGAATAG CAAGCAGATC 
ATATGGTTCT GGCAGTTTGT GAAGGAAATG GACAATGAGG TGCGGTTGCG GCTGCTGCAG 
TTTGTCACTG GTACCTGCAG GCTGCCGCTG GGAGGATTCG CAGAGCTCAT GGGAAGCAAC 
GGTCCACAGA AATTCTGCAT TGAGAAGGTT GGAAAAGAAA CATGGCTCCC TCGAAGTCAT 
ACATGTTTCA ATCGACTGGA CCTGCCGCCA TATAAAAGCT ATGAACAACT GAAGGAGAAG 
CTGCTTTTTG CAATTGAAGA AACGGAAGGA TTTGGCCAAG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819605.1
CDS	428..3172
Translation	MATASPRPGNRNSLEDLQLHVTVSCAKLKRKKNWFGTTVYTELAVDGETKKTAKSSSSSNPKWEERLTVSVTPQTTLEFKVWSHHSWKADALLGKTAVDLKQALETHNRKLERVKEQLKLSLDGKHGTVQTGELTVILDGLNVEPESLATFNSTATPAREIQQNGDAVRENGGETSLEARVGATCDMPNGTASETTSEQNAFNTQIANIDGTHSPSRVAARPKNTPAPNPLTGDRTVNGTSATSEANSTPSHEALPSPAGAPSSDSIEMMDSTSDGPVSIPLDSGTSAESSSAGASSEPVRAADSGPSPAAGSNSPDAAKPRQASSTGSGDPVRQQPSSAAEPLPSGWEQRKDPHGRTYYVDHNTRTTTWERPQPLPPGWERRVDDRGRVYYVDHNTRTTTWQRPTMESVRNFEQWQSQRNQLQGAMQQFNQRYLYSASMLSAENDPLGPLPPGWERRVDSTDRVYFVNHNTKTTQWEDPRTQGLQNEDPLPEGWEIRYTREGVKYFVDHNTKTTTFSDPRTGKSAVSKGPQIAYERSFRWKLAHFRYLCQSNALPSHVKITVSRQTLFEDSFQQIMALKPYDLRRRLYVMFRGEEGLDYGGLAREWFFLLSHEVLNPMYCLFEYAGKSNYCLQINPASTINPDHLSYFCFIGRFIAMALFHGKFIDTGFSLPFYKRMLSKKLTIKDLESIDPEFYNSLIWIRDNNIEECNLEMYFSVDMEILGKVTSHDLKPEGSNILVTEENKEEYIGLMAEWRFSRGVEEQTKAFLDGFNAVVPLQWLQYFDEKELEVMLCGMQEVDLSDWQRNTVYRHYTRNSKQIIWFWQFVKEMDNEVRLRLLQFVTGTCRLPLGGFAELMGSNGPQKFCIEKVGKETWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	XM_012964151.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis WW domain containing E3 ubiquitin protein ligase 1 (wwp1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012964151.2

ORF Insert Sequence:

ATGGCCACAG CCTCGCCACG GCCGGGGAAT CGAAACAGTC TTGAAGATTT ACAGCTGCAC 
GTGACAGTGT CCTGTGCCAA ACTGAAGCGG AAGAAGAACT GGTTTGGAAC GACTGTATAT 
ACAGAATTAG CTGTTGATGG TGAAACAAAG AAGACTGCAA AATCAAGCAG CTCGAGTAAC 
CCCAAATGGG AAGAACGGTT AACTGTGAGT GTGACCCCAC AAACTACACT TGAATTCAAG 
GTATGGAGCC ACCATTCGTG GAAAGCGGAT GCGTTGCTTG GCAAAACCGC TGTCGACTTA 
AAGCAAGCTC TGGAGACCCA TAACCGGAAG TTGGAAAGAG TGAAAGAACA ACTGAAACTT 
TCATTGGACG GCAAACATGG CACAGTACAG ACTGGCGAGC TGACAGTCAT TTTAGATGGA 
TTAAATGTGG AGCCTGAATC ACTTGCTACG TTTAACTCAA CAGCCACTCC AGCCAGAGAA 
ATCCAGCAGA ATGGGGATGC CGTCCGTGAG AATGGAGGAG AAACCTCTTT GGAAGCAAGA 
GTTGGAGCCA CTTGCGACAT GCCCAATGGG ACAGCTAGTG AAACCACATC CGAGCAGAAT 
GCCTTTAATA CACAAATAGC AAATATAGAT GGCACTCATT CCCCTTCTCG TGTTGCAGCC 
AGACCCAAAA ATACACCAGC ACCAAATCCA CTGACTGGCG ATAGAACTGT TAATGGCACA 
AGTGCTACAT CTGAAGCAAA TAGCACACCT TCCCATGAAG CTTTACCATC TCCTGCCGGA 
GCCCCCTCAT CAGATAGCAT AGAGATGATG GACAGTACAT CAGATGGGCC AGTATCAATA 
CCTTTAGATA GCGGCACGTC TGCAGAGAGC AGTTCTGCTG GGGCTTCATC AGAACCAGTA 
AGGGCTGCAG ACTCTGGGCC ATCACCTGCT GCTGGCAGCA ATAGCCCTGA TGCTGCAAAA 
CCAAGGCAGG CGTCATCTAC AGGTTCTGGG GATCCTGTCA GACAGCAGCC AAGCTCCGCT 
GCAGAGCCTT TGCCATCGGG ATGGGAACAG AGGAAAGACC CCCATGGTAG AACCTACTAC 
GTTGATCACA ACACAAGGAC TACAACATGG GAACGCCCAC AGCCTTTGCC TCCTGGGTGG 
GAGAGAAGGG TCGATGATCG TGGGAGAGTA TATTATGTTG ACCATAACAC AAGAACCACA 
ACATGGCAGA GGCCTACTAT GGAGTCTGTA CGAAACTTTG AACAGTGGCA GTCTCAGCGC 
AATCAACTCC AGGGAGCTAT GCAGCAGTTT AACCAGCGAT ACCTCTATTC GGCATCAATG 
TTATCCGCTG AAAATGACCC GTTAGGACCA CTTCCGCCAG GATGGGAGAG AAGAGTGGAT 
TCCACTGATC GTGTCTATTT TGTGAACCAC AATACAAAAA CAACACAGTG GGAGGACCCT 
CGAACTCAAG GTTTACAGAA CGAAGATCCA TTGCCAGAGG GCTGGGAAAT CCGCTACACA 
AGAGAAGGAG TAAAGTACTT TGTAGATCAC AATACAAAGA CGACTACTTT CAGCGACCCA 
AGAACTGGGA AGTCAGCAGT AAGCAAAGGG CCACAGATTG CCTATGAACG TAGTTTCCGC 
TGGAAGCTTG CTCATTTTCG TTATTTATGC CAGTCCAATG CACTGCCCAG CCATGTGAAG 
ATAACGGTGT CCAGGCAGAC GCTCTTTGAA GACTCTTTCC AGCAGATTAT GGCATTAAAA 
CCATATGATC TGAGAAGGCG ATTGTATGTC ATGTTCAGAG GGGAAGAAGG TCTGGACTAT 
GGAGGCTTAG CCAGAGAGTG GTTTTTCCTG CTTTCCCATG AGGTACTGAA CCCCATGTAC 
TGCCTGTTCG AGTATGCAGG CAAGAGCAAT TACTGCTTGC AGATCAACCC TGCCTCCACC 
ATTAATCCTG ACCACCTCTC CTATTTCTGC TTTATTGGCC GCTTTATTGC AATGGCGCTG 
TTCCATGGGA AGTTTATTGA TACTGGCTTC TCTCTTCCAT TCTACAAACG AATGCTCAGC 
AAGAAACTTA CCATTAAAGA TCTGGAATCG ATTGATCCCG AGTTCTACAA CTCTTTGATA 
TGGATACGGG ACAATAACAT TGAAGAATGT AACCTGGAAA TGTACTTTTC TGTGGACATG 
GAAATATTGG GGAAAGTCAC CTCACATGAT CTGAAGCCTG AAGGTTCCAA CATCCTTGTG 
ACTGAGGAGA ACAAGGAAGA ATATATTGGT CTCATGGCAG AATGGAGATT CTCACGTGGA 
GTTGAAGAAC AAACCAAAGC ATTTCTTGAT GGCTTTAATG CAGTGGTCCC ACTTCAGTGG 
CTACAGTACT TTGATGAAAA AGAGTTAGAG GTTATGCTGT GTGGCATGCA GGAGGTGGAT 
TTATCAGACT GGCAGCGGAA TACAGTCTAT CGGCATTACA CAAGGAATAG CAAGCAGATC 
ATATGGTTCT GGCAGTTTGT GAAGGAAATG GACAATGAGG TGCGGTTGCG GCTGCTGCAG 
TTTGTCACTG GTACCTGCAG GCTGCCGCTG GGAGGATTCG CAGAGCTCAT GGGAAGCAAC 
GGTCCACAGA AATTCTGCAT TGAGAAGGTT GGAAAAGAAA CATGGCTCCC TCGAAGTCAT 
ACATGTTTCA ATCGACTGGA CCTGCCGCCA TATAAAAGCT ATGAACAACT GAAGGAGAAG 
CTGCTTTTTG CAATTGAAGA AACGGAAGGA TTTGGCCAAG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wwp1 ( XM_012964150.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819604.1 Xenopus tropicalis WW domain containing E3 ubiquitin protein ligase 1 (wwp1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa30166
Clone ID Related Accession (Same CDS sequence)	XM_012964151.2 , XM_012964150.2 , XM_012964152.2
Accession Version	XM_012964150.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	NEDD4-like E3 ubiquitin-protein ligase WWP1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012964150.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG CCTCGCCACG GCCGGGGAAT CGAAACAGTC TTGAAGATTT ACAGCTGCAC 
GTGACAGTGT CCTGTGCCAA ACTGAAGCGG AAGAAGAACT GGTTTGGAAC GACTGTATAT 
ACAGAATTAG CTGTTGATGG TGAAACAAAG AAGACTGCAA AATCAAGCAG CTCGAGTAAC 
CCCAAATGGG AAGAACGGTT AACTGTGAGT GTGACCCCAC AAACTACACT TGAATTCAAG 
GTATGGAGCC ACCATTCGTG GAAAGCGGAT GCGTTGCTTG GCAAAACCGC TGTCGACTTA 
AAGCAAGCTC TGGAGACCCA TAACCGGAAG TTGGAAAGAG TGAAAGAACA ACTGAAACTT 
TCATTGGACG GCAAACATGG CACAGTACAG ACTGGCGAGC TGACAGTCAT TTTAGATGGA 
TTAAATGTGG AGCCTGAATC ACTTGCTACG TTTAACTCAA CAGCCACTCC AGCCAGAGAA 
ATCCAGCAGA ATGGGGATGC CGTCCGTGAG AATGGAGGAG AAACCTCTTT GGAAGCAAGA 
GTTGGAGCCA CTTGCGACAT GCCCAATGGG ACAGCTAGTG AAACCACATC CGAGCAGAAT 
GCCTTTAATA CACAAATAGC AAATATAGAT GGCACTCATT CCCCTTCTCG TGTTGCAGCC 
AGACCCAAAA ATACACCAGC ACCAAATCCA CTGACTGGCG ATAGAACTGT TAATGGCACA 
AGTGCTACAT CTGAAGCAAA TAGCACACCT TCCCATGAAG CTTTACCATC TCCTGCCGGA 
GCCCCCTCAT CAGATAGCAT AGAGATGATG GACAGTACAT CAGATGGGCC AGTATCAATA 
CCTTTAGATA GCGGCACGTC TGCAGAGAGC AGTTCTGCTG GGGCTTCATC AGAACCAGTA 
AGGGCTGCAG ACTCTGGGCC ATCACCTGCT GCTGGCAGCA ATAGCCCTGA TGCTGCAAAA 
CCAAGGCAGG CGTCATCTAC AGGTTCTGGG GATCCTGTCA GACAGCAGCC AAGCTCCGCT 
GCAGAGCCTT TGCCATCGGG ATGGGAACAG AGGAAAGACC CCCATGGTAG AACCTACTAC 
GTTGATCACA ACACAAGGAC TACAACATGG GAACGCCCAC AGCCTTTGCC TCCTGGGTGG 
GAGAGAAGGG TCGATGATCG TGGGAGAGTA TATTATGTTG ACCATAACAC AAGAACCACA 
ACATGGCAGA GGCCTACTAT GGAGTCTGTA CGAAACTTTG AACAGTGGCA GTCTCAGCGC 
AATCAACTCC AGGGAGCTAT GCAGCAGTTT AACCAGCGAT ACCTCTATTC GGCATCAATG 
TTATCCGCTG AAAATGACCC GTTAGGACCA CTTCCGCCAG GATGGGAGAG AAGAGTGGAT 
TCCACTGATC GTGTCTATTT TGTGAACCAC AATACAAAAA CAACACAGTG GGAGGACCCT 
CGAACTCAAG GTTTACAGAA CGAAGATCCA TTGCCAGAGG GCTGGGAAAT CCGCTACACA 
AGAGAAGGAG TAAAGTACTT TGTAGATCAC AATACAAAGA CGACTACTTT CAGCGACCCA 
AGAACTGGGA AGTCAGCAGT AAGCAAAGGG CCACAGATTG CCTATGAACG TAGTTTCCGC 
TGGAAGCTTG CTCATTTTCG TTATTTATGC CAGTCCAATG CACTGCCCAG CCATGTGAAG 
ATAACGGTGT CCAGGCAGAC GCTCTTTGAA GACTCTTTCC AGCAGATTAT GGCATTAAAA 
CCATATGATC TGAGAAGGCG ATTGTATGTC ATGTTCAGAG GGGAAGAAGG TCTGGACTAT 
GGAGGCTTAG CCAGAGAGTG GTTTTTCCTG CTTTCCCATG AGGTACTGAA CCCCATGTAC 
TGCCTGTTCG AGTATGCAGG CAAGAGCAAT TACTGCTTGC AGATCAACCC TGCCTCCACC 
ATTAATCCTG ACCACCTCTC CTATTTCTGC TTTATTGGCC GCTTTATTGC AATGGCGCTG 
TTCCATGGGA AGTTTATTGA TACTGGCTTC TCTCTTCCAT TCTACAAACG AATGCTCAGC 
AAGAAACTTA CCATTAAAGA TCTGGAATCG ATTGATCCCG AGTTCTACAA CTCTTTGATA 
TGGATACGGG ACAATAACAT TGAAGAATGT AACCTGGAAA TGTACTTTTC TGTGGACATG 
GAAATATTGG GGAAAGTCAC CTCACATGAT CTGAAGCCTG AAGGTTCCAA CATCCTTGTG 
ACTGAGGAGA ACAAGGAAGA ATATATTGGT CTCATGGCAG AATGGAGATT CTCACGTGGA 
GTTGAAGAAC AAACCAAAGC ATTTCTTGAT GGCTTTAATG CAGTGGTCCC ACTTCAGTGG 
CTACAGTACT TTGATGAAAA AGAGTTAGAG GTTATGCTGT GTGGCATGCA GGAGGTGGAT 
TTATCAGACT GGCAGCGGAA TACAGTCTAT CGGCATTACA CAAGGAATAG CAAGCAGATC 
ATATGGTTCT GGCAGTTTGT GAAGGAAATG GACAATGAGG TGCGGTTGCG GCTGCTGCAG 
TTTGTCACTG GTACCTGCAG GCTGCCGCTG GGAGGATTCG CAGAGCTCAT GGGAAGCAAC 
GGTCCACAGA AATTCTGCAT TGAGAAGGTT GGAAAAGAAA CATGGCTCCC TCGAAGTCAT 
ACATGTTTCA ATCGACTGGA CCTGCCGCCA TATAAAAGCT ATGAACAACT GAAGGAGAAG 
CTGCTTTTTG CAATTGAAGA AACGGAAGGA TTTGGCCAAG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819604.1
CDS	355..3099
Translation	MATASPRPGNRNSLEDLQLHVTVSCAKLKRKKNWFGTTVYTELAVDGETKKTAKSSSSSNPKWEERLTVSVTPQTTLEFKVWSHHSWKADALLGKTAVDLKQALETHNRKLERVKEQLKLSLDGKHGTVQTGELTVILDGLNVEPESLATFNSTATPAREIQQNGDAVRENGGETSLEARVGATCDMPNGTASETTSEQNAFNTQIANIDGTHSPSRVAARPKNTPAPNPLTGDRTVNGTSATSEANSTPSHEALPSPAGAPSSDSIEMMDSTSDGPVSIPLDSGTSAESSSAGASSEPVRAADSGPSPAAGSNSPDAAKPRQASSTGSGDPVRQQPSSAAEPLPSGWEQRKDPHGRTYYVDHNTRTTTWERPQPLPPGWERRVDDRGRVYYVDHNTRTTTWQRPTMESVRNFEQWQSQRNQLQGAMQQFNQRYLYSASMLSAENDPLGPLPPGWERRVDSTDRVYFVNHNTKTTQWEDPRTQGLQNEDPLPEGWEIRYTREGVKYFVDHNTKTTTFSDPRTGKSAVSKGPQIAYERSFRWKLAHFRYLCQSNALPSHVKITVSRQTLFEDSFQQIMALKPYDLRRRLYVMFRGEEGLDYGGLAREWFFLLSHEVLNPMYCLFEYAGKSNYCLQINPASTINPDHLSYFCFIGRFIAMALFHGKFIDTGFSLPFYKRMLSKKLTIKDLESIDPEFYNSLIWIRDNNIEECNLEMYFSVDMEILGKVTSHDLKPEGSNILVTEENKEEYIGLMAEWRFSRGVEEQTKAFLDGFNAVVPLQWLQYFDEKELEVMLCGMQEVDLSDWQRNTVYRHYTRNSKQIIWFWQFVKEMDNEVRLRLLQFVTGTCRLPLGGFAELMGSNGPQKFCIEKVGKETWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	XM_012964150.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis WW domain containing E3 ubiquitin protein ligase 1 (wwp1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012964150.2

ORF Insert Sequence:

ATGGCCACAG CCTCGCCACG GCCGGGGAAT CGAAACAGTC TTGAAGATTT ACAGCTGCAC 
GTGACAGTGT CCTGTGCCAA ACTGAAGCGG AAGAAGAACT GGTTTGGAAC GACTGTATAT 
ACAGAATTAG CTGTTGATGG TGAAACAAAG AAGACTGCAA AATCAAGCAG CTCGAGTAAC 
CCCAAATGGG AAGAACGGTT AACTGTGAGT GTGACCCCAC AAACTACACT TGAATTCAAG 
GTATGGAGCC ACCATTCGTG GAAAGCGGAT GCGTTGCTTG GCAAAACCGC TGTCGACTTA 
AAGCAAGCTC TGGAGACCCA TAACCGGAAG TTGGAAAGAG TGAAAGAACA ACTGAAACTT 
TCATTGGACG GCAAACATGG CACAGTACAG ACTGGCGAGC TGACAGTCAT TTTAGATGGA 
TTAAATGTGG AGCCTGAATC ACTTGCTACG TTTAACTCAA CAGCCACTCC AGCCAGAGAA 
ATCCAGCAGA ATGGGGATGC CGTCCGTGAG AATGGAGGAG AAACCTCTTT GGAAGCAAGA 
GTTGGAGCCA CTTGCGACAT GCCCAATGGG ACAGCTAGTG AAACCACATC CGAGCAGAAT 
GCCTTTAATA CACAAATAGC AAATATAGAT GGCACTCATT CCCCTTCTCG TGTTGCAGCC 
AGACCCAAAA ATACACCAGC ACCAAATCCA CTGACTGGCG ATAGAACTGT TAATGGCACA 
AGTGCTACAT CTGAAGCAAA TAGCACACCT TCCCATGAAG CTTTACCATC TCCTGCCGGA 
GCCCCCTCAT CAGATAGCAT AGAGATGATG GACAGTACAT CAGATGGGCC AGTATCAATA 
CCTTTAGATA GCGGCACGTC TGCAGAGAGC AGTTCTGCTG GGGCTTCATC AGAACCAGTA 
AGGGCTGCAG ACTCTGGGCC ATCACCTGCT GCTGGCAGCA ATAGCCCTGA TGCTGCAAAA 
CCAAGGCAGG CGTCATCTAC AGGTTCTGGG GATCCTGTCA GACAGCAGCC AAGCTCCGCT 
GCAGAGCCTT TGCCATCGGG ATGGGAACAG AGGAAAGACC CCCATGGTAG AACCTACTAC 
GTTGATCACA ACACAAGGAC TACAACATGG GAACGCCCAC AGCCTTTGCC TCCTGGGTGG 
GAGAGAAGGG TCGATGATCG TGGGAGAGTA TATTATGTTG ACCATAACAC AAGAACCACA 
ACATGGCAGA GGCCTACTAT GGAGTCTGTA CGAAACTTTG AACAGTGGCA GTCTCAGCGC 
AATCAACTCC AGGGAGCTAT GCAGCAGTTT AACCAGCGAT ACCTCTATTC GGCATCAATG 
TTATCCGCTG AAAATGACCC GTTAGGACCA CTTCCGCCAG GATGGGAGAG AAGAGTGGAT 
TCCACTGATC GTGTCTATTT TGTGAACCAC AATACAAAAA CAACACAGTG GGAGGACCCT 
CGAACTCAAG GTTTACAGAA CGAAGATCCA TTGCCAGAGG GCTGGGAAAT CCGCTACACA 
AGAGAAGGAG TAAAGTACTT TGTAGATCAC AATACAAAGA CGACTACTTT CAGCGACCCA 
AGAACTGGGA AGTCAGCAGT AAGCAAAGGG CCACAGATTG CCTATGAACG TAGTTTCCGC 
TGGAAGCTTG CTCATTTTCG TTATTTATGC CAGTCCAATG CACTGCCCAG CCATGTGAAG 
ATAACGGTGT CCAGGCAGAC GCTCTTTGAA GACTCTTTCC AGCAGATTAT GGCATTAAAA 
CCATATGATC TGAGAAGGCG ATTGTATGTC ATGTTCAGAG GGGAAGAAGG TCTGGACTAT 
GGAGGCTTAG CCAGAGAGTG GTTTTTCCTG CTTTCCCATG AGGTACTGAA CCCCATGTAC 
TGCCTGTTCG AGTATGCAGG CAAGAGCAAT TACTGCTTGC AGATCAACCC TGCCTCCACC 
ATTAATCCTG ACCACCTCTC CTATTTCTGC TTTATTGGCC GCTTTATTGC AATGGCGCTG 
TTCCATGGGA AGTTTATTGA TACTGGCTTC TCTCTTCCAT TCTACAAACG AATGCTCAGC 
AAGAAACTTA CCATTAAAGA TCTGGAATCG ATTGATCCCG AGTTCTACAA CTCTTTGATA 
TGGATACGGG ACAATAACAT TGAAGAATGT AACCTGGAAA TGTACTTTTC TGTGGACATG 
GAAATATTGG GGAAAGTCAC CTCACATGAT CTGAAGCCTG AAGGTTCCAA CATCCTTGTG 
ACTGAGGAGA ACAAGGAAGA ATATATTGGT CTCATGGCAG AATGGAGATT CTCACGTGGA 
GTTGAAGAAC AAACCAAAGC ATTTCTTGAT GGCTTTAATG CAGTGGTCCC ACTTCAGTGG 
CTACAGTACT TTGATGAAAA AGAGTTAGAG GTTATGCTGT GTGGCATGCA GGAGGTGGAT 
TTATCAGACT GGCAGCGGAA TACAGTCTAT CGGCATTACA CAAGGAATAG CAAGCAGATC 
ATATGGTTCT GGCAGTTTGT GAAGGAAATG GACAATGAGG TGCGGTTGCG GCTGCTGCAG 
TTTGTCACTG GTACCTGCAG GCTGCCGCTG GGAGGATTCG CAGAGCTCAT GGGAAGCAAC 
GGTCCACAGA AATTCTGCAT TGAGAAGGTT GGAAAAGAAA CATGGCTCCC TCGAAGTCAT 
ACATGTTTCA ATCGACTGGA CCTGCCGCCA TATAAAAGCT ATGAACAACT GAAGGAGAAG 
CTGCTTTTTG CAATTGAAGA AACGGAAGGA TTTGGCCAAG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wwp1 ( XM_012964152.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819606.1 Xenopus tropicalis WW domain containing E3 ubiquitin protein ligase 1 (wwp1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa30166
Clone ID Related Accession (Same CDS sequence)	XM_012964151.2 , XM_012964150.2 , XM_012964152.2
Accession Version	XM_012964152.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	NEDD4-like E3 ubiquitin-protein ligase WWP1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012964152.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG CCTCGCCACG GCCGGGGAAT CGAAACAGTC TTGAAGATTT ACAGCTGCAC 
GTGACAGTGT CCTGTGCCAA ACTGAAGCGG AAGAAGAACT GGTTTGGAAC GACTGTATAT 
ACAGAATTAG CTGTTGATGG TGAAACAAAG AAGACTGCAA AATCAAGCAG CTCGAGTAAC 
CCCAAATGGG AAGAACGGTT AACTGTGAGT GTGACCCCAC AAACTACACT TGAATTCAAG 
GTATGGAGCC ACCATTCGTG GAAAGCGGAT GCGTTGCTTG GCAAAACCGC TGTCGACTTA 
AAGCAAGCTC TGGAGACCCA TAACCGGAAG TTGGAAAGAG TGAAAGAACA ACTGAAACTT 
TCATTGGACG GCAAACATGG CACAGTACAG ACTGGCGAGC TGACAGTCAT TTTAGATGGA 
TTAAATGTGG AGCCTGAATC ACTTGCTACG TTTAACTCAA CAGCCACTCC AGCCAGAGAA 
ATCCAGCAGA ATGGGGATGC CGTCCGTGAG AATGGAGGAG AAACCTCTTT GGAAGCAAGA 
GTTGGAGCCA CTTGCGACAT GCCCAATGGG ACAGCTAGTG AAACCACATC CGAGCAGAAT 
GCCTTTAATA CACAAATAGC AAATATAGAT GGCACTCATT CCCCTTCTCG TGTTGCAGCC 
AGACCCAAAA ATACACCAGC ACCAAATCCA CTGACTGGCG ATAGAACTGT TAATGGCACA 
AGTGCTACAT CTGAAGCAAA TAGCACACCT TCCCATGAAG CTTTACCATC TCCTGCCGGA 
GCCCCCTCAT CAGATAGCAT AGAGATGATG GACAGTACAT CAGATGGGCC AGTATCAATA 
CCTTTAGATA GCGGCACGTC TGCAGAGAGC AGTTCTGCTG GGGCTTCATC AGAACCAGTA 
AGGGCTGCAG ACTCTGGGCC ATCACCTGCT GCTGGCAGCA ATAGCCCTGA TGCTGCAAAA 
CCAAGGCAGG CGTCATCTAC AGGTTCTGGG GATCCTGTCA GACAGCAGCC AAGCTCCGCT 
GCAGAGCCTT TGCCATCGGG ATGGGAACAG AGGAAAGACC CCCATGGTAG AACCTACTAC 
GTTGATCACA ACACAAGGAC TACAACATGG GAACGCCCAC AGCCTTTGCC TCCTGGGTGG 
GAGAGAAGGG TCGATGATCG TGGGAGAGTA TATTATGTTG ACCATAACAC AAGAACCACA 
ACATGGCAGA GGCCTACTAT GGAGTCTGTA CGAAACTTTG AACAGTGGCA GTCTCAGCGC 
AATCAACTCC AGGGAGCTAT GCAGCAGTTT AACCAGCGAT ACCTCTATTC GGCATCAATG 
TTATCCGCTG AAAATGACCC GTTAGGACCA CTTCCGCCAG GATGGGAGAG AAGAGTGGAT 
TCCACTGATC GTGTCTATTT TGTGAACCAC AATACAAAAA CAACACAGTG GGAGGACCCT 
CGAACTCAAG GTTTACAGAA CGAAGATCCA TTGCCAGAGG GCTGGGAAAT CCGCTACACA 
AGAGAAGGAG TAAAGTACTT TGTAGATCAC AATACAAAGA CGACTACTTT CAGCGACCCA 
AGAACTGGGA AGTCAGCAGT AAGCAAAGGG CCACAGATTG CCTATGAACG TAGTTTCCGC 
TGGAAGCTTG CTCATTTTCG TTATTTATGC CAGTCCAATG CACTGCCCAG CCATGTGAAG 
ATAACGGTGT CCAGGCAGAC GCTCTTTGAA GACTCTTTCC AGCAGATTAT GGCATTAAAA 
CCATATGATC TGAGAAGGCG ATTGTATGTC ATGTTCAGAG GGGAAGAAGG TCTGGACTAT 
GGAGGCTTAG CCAGAGAGTG GTTTTTCCTG CTTTCCCATG AGGTACTGAA CCCCATGTAC 
TGCCTGTTCG AGTATGCAGG CAAGAGCAAT TACTGCTTGC AGATCAACCC TGCCTCCACC 
ATTAATCCTG ACCACCTCTC CTATTTCTGC TTTATTGGCC GCTTTATTGC AATGGCGCTG 
TTCCATGGGA AGTTTATTGA TACTGGCTTC TCTCTTCCAT TCTACAAACG AATGCTCAGC 
AAGAAACTTA CCATTAAAGA TCTGGAATCG ATTGATCCCG AGTTCTACAA CTCTTTGATA 
TGGATACGGG ACAATAACAT TGAAGAATGT AACCTGGAAA TGTACTTTTC TGTGGACATG 
GAAATATTGG GGAAAGTCAC CTCACATGAT CTGAAGCCTG AAGGTTCCAA CATCCTTGTG 
ACTGAGGAGA ACAAGGAAGA ATATATTGGT CTCATGGCAG AATGGAGATT CTCACGTGGA 
GTTGAAGAAC AAACCAAAGC ATTTCTTGAT GGCTTTAATG CAGTGGTCCC ACTTCAGTGG 
CTACAGTACT TTGATGAAAA AGAGTTAGAG GTTATGCTGT GTGGCATGCA GGAGGTGGAT 
TTATCAGACT GGCAGCGGAA TACAGTCTAT CGGCATTACA CAAGGAATAG CAAGCAGATC 
ATATGGTTCT GGCAGTTTGT GAAGGAAATG GACAATGAGG TGCGGTTGCG GCTGCTGCAG 
TTTGTCACTG GTACCTGCAG GCTGCCGCTG GGAGGATTCG CAGAGCTCAT GGGAAGCAAC 
GGTCCACAGA AATTCTGCAT TGAGAAGGTT GGAAAAGAAA CATGGCTCCC TCGAAGTCAT 
ACATGTTTCA ATCGACTGGA CCTGCCGCCA TATAAAAGCT ATGAACAACT GAAGGAGAAG 
CTGCTTTTTG CAATTGAAGA AACGGAAGGA TTTGGCCAAG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819606.1
CDS	54..2798
Translation	MATASPRPGNRNSLEDLQLHVTVSCAKLKRKKNWFGTTVYTELAVDGETKKTAKSSSSSNPKWEERLTVSVTPQTTLEFKVWSHHSWKADALLGKTAVDLKQALETHNRKLERVKEQLKLSLDGKHGTVQTGELTVILDGLNVEPESLATFNSTATPAREIQQNGDAVRENGGETSLEARVGATCDMPNGTASETTSEQNAFNTQIANIDGTHSPSRVAARPKNTPAPNPLTGDRTVNGTSATSEANSTPSHEALPSPAGAPSSDSIEMMDSTSDGPVSIPLDSGTSAESSSAGASSEPVRAADSGPSPAAGSNSPDAAKPRQASSTGSGDPVRQQPSSAAEPLPSGWEQRKDPHGRTYYVDHNTRTTTWERPQPLPPGWERRVDDRGRVYYVDHNTRTTTWQRPTMESVRNFEQWQSQRNQLQGAMQQFNQRYLYSASMLSAENDPLGPLPPGWERRVDSTDRVYFVNHNTKTTQWEDPRTQGLQNEDPLPEGWEIRYTREGVKYFVDHNTKTTTFSDPRTGKSAVSKGPQIAYERSFRWKLAHFRYLCQSNALPSHVKITVSRQTLFEDSFQQIMALKPYDLRRRLYVMFRGEEGLDYGGLAREWFFLLSHEVLNPMYCLFEYAGKSNYCLQINPASTINPDHLSYFCFIGRFIAMALFHGKFIDTGFSLPFYKRMLSKKLTIKDLESIDPEFYNSLIWIRDNNIEECNLEMYFSVDMEILGKVTSHDLKPEGSNILVTEENKEEYIGLMAEWRFSRGVEEQTKAFLDGFNAVVPLQWLQYFDEKELEVMLCGMQEVDLSDWQRNTVYRHYTRNSKQIIWFWQFVKEMDNEVRLRLLQFVTGTCRLPLGGFAELMGSNGPQKFCIEKVGKETWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	XM_012964152.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis WW domain containing E3 ubiquitin protein ligase 1 (wwp1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012964152.2

ORF Insert Sequence:

ATGGCCACAG CCTCGCCACG GCCGGGGAAT CGAAACAGTC TTGAAGATTT ACAGCTGCAC 
GTGACAGTGT CCTGTGCCAA ACTGAAGCGG AAGAAGAACT GGTTTGGAAC GACTGTATAT 
ACAGAATTAG CTGTTGATGG TGAAACAAAG AAGACTGCAA AATCAAGCAG CTCGAGTAAC 
CCCAAATGGG AAGAACGGTT AACTGTGAGT GTGACCCCAC AAACTACACT TGAATTCAAG 
GTATGGAGCC ACCATTCGTG GAAAGCGGAT GCGTTGCTTG GCAAAACCGC TGTCGACTTA 
AAGCAAGCTC TGGAGACCCA TAACCGGAAG TTGGAAAGAG TGAAAGAACA ACTGAAACTT 
TCATTGGACG GCAAACATGG CACAGTACAG ACTGGCGAGC TGACAGTCAT TTTAGATGGA 
TTAAATGTGG AGCCTGAATC ACTTGCTACG TTTAACTCAA CAGCCACTCC AGCCAGAGAA 
ATCCAGCAGA ATGGGGATGC CGTCCGTGAG AATGGAGGAG AAACCTCTTT GGAAGCAAGA 
GTTGGAGCCA CTTGCGACAT GCCCAATGGG ACAGCTAGTG AAACCACATC CGAGCAGAAT 
GCCTTTAATA CACAAATAGC AAATATAGAT GGCACTCATT CCCCTTCTCG TGTTGCAGCC 
AGACCCAAAA ATACACCAGC ACCAAATCCA CTGACTGGCG ATAGAACTGT TAATGGCACA 
AGTGCTACAT CTGAAGCAAA TAGCACACCT TCCCATGAAG CTTTACCATC TCCTGCCGGA 
GCCCCCTCAT CAGATAGCAT AGAGATGATG GACAGTACAT CAGATGGGCC AGTATCAATA 
CCTTTAGATA GCGGCACGTC TGCAGAGAGC AGTTCTGCTG GGGCTTCATC AGAACCAGTA 
AGGGCTGCAG ACTCTGGGCC ATCACCTGCT GCTGGCAGCA ATAGCCCTGA TGCTGCAAAA 
CCAAGGCAGG CGTCATCTAC AGGTTCTGGG GATCCTGTCA GACAGCAGCC AAGCTCCGCT 
GCAGAGCCTT TGCCATCGGG ATGGGAACAG AGGAAAGACC CCCATGGTAG AACCTACTAC 
GTTGATCACA ACACAAGGAC TACAACATGG GAACGCCCAC AGCCTTTGCC TCCTGGGTGG 
GAGAGAAGGG TCGATGATCG TGGGAGAGTA TATTATGTTG ACCATAACAC AAGAACCACA 
ACATGGCAGA GGCCTACTAT GGAGTCTGTA CGAAACTTTG AACAGTGGCA GTCTCAGCGC 
AATCAACTCC AGGGAGCTAT GCAGCAGTTT AACCAGCGAT ACCTCTATTC GGCATCAATG 
TTATCCGCTG AAAATGACCC GTTAGGACCA CTTCCGCCAG GATGGGAGAG AAGAGTGGAT 
TCCACTGATC GTGTCTATTT TGTGAACCAC AATACAAAAA CAACACAGTG GGAGGACCCT 
CGAACTCAAG GTTTACAGAA CGAAGATCCA TTGCCAGAGG GCTGGGAAAT CCGCTACACA 
AGAGAAGGAG TAAAGTACTT TGTAGATCAC AATACAAAGA CGACTACTTT CAGCGACCCA 
AGAACTGGGA AGTCAGCAGT AAGCAAAGGG CCACAGATTG CCTATGAACG TAGTTTCCGC 
TGGAAGCTTG CTCATTTTCG TTATTTATGC CAGTCCAATG CACTGCCCAG CCATGTGAAG 
ATAACGGTGT CCAGGCAGAC GCTCTTTGAA GACTCTTTCC AGCAGATTAT GGCATTAAAA 
CCATATGATC TGAGAAGGCG ATTGTATGTC ATGTTCAGAG GGGAAGAAGG TCTGGACTAT 
GGAGGCTTAG CCAGAGAGTG GTTTTTCCTG CTTTCCCATG AGGTACTGAA CCCCATGTAC 
TGCCTGTTCG AGTATGCAGG CAAGAGCAAT TACTGCTTGC AGATCAACCC TGCCTCCACC 
ATTAATCCTG ACCACCTCTC CTATTTCTGC TTTATTGGCC GCTTTATTGC AATGGCGCTG 
TTCCATGGGA AGTTTATTGA TACTGGCTTC TCTCTTCCAT TCTACAAACG AATGCTCAGC 
AAGAAACTTA CCATTAAAGA TCTGGAATCG ATTGATCCCG AGTTCTACAA CTCTTTGATA 
TGGATACGGG ACAATAACAT TGAAGAATGT AACCTGGAAA TGTACTTTTC TGTGGACATG 
GAAATATTGG GGAAAGTCAC CTCACATGAT CTGAAGCCTG AAGGTTCCAA CATCCTTGTG 
ACTGAGGAGA ACAAGGAAGA ATATATTGGT CTCATGGCAG AATGGAGATT CTCACGTGGA 
GTTGAAGAAC AAACCAAAGC ATTTCTTGAT GGCTTTAATG CAGTGGTCCC ACTTCAGTGG 
CTACAGTACT TTGATGAAAA AGAGTTAGAG GTTATGCTGT GTGGCATGCA GGAGGTGGAT 
TTATCAGACT GGCAGCGGAA TACAGTCTAT CGGCATTACA CAAGGAATAG CAAGCAGATC 
ATATGGTTCT GGCAGTTTGT GAAGGAAATG GACAATGAGG TGCGGTTGCG GCTGCTGCAG 
TTTGTCACTG GTACCTGCAG GCTGCCGCTG GGAGGATTCG CAGAGCTCAT GGGAAGCAAC 
GGTCCACAGA AATTCTGCAT TGAGAAGGTT GGAAAAGAAA CATGGCTCCC TCGAAGTCAT 
ACATGTTTCA ATCGACTGGA CCTGCCGCCA TATAAAAGCT ATGAACAACT GAAGGAGAAG 
CTGCTTTTTG CAATTGAAGA AACGGAAGGA TTTGGCCAAG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wwp1 ( XM_012964151.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819605.2 Xenopus tropicalis WW domain containing E3 ubiquitin protein ligase 1 (wwp1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa24470
Clone ID Related Accession (Same CDS sequence)	NM_001097375.1 , XM_012964151.3 , XM_012964150.3 , XM_012964152.3
Accession Version	XM_012964151.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	NEDD4-like E3 ubiquitin-protein ligase WWP1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012964151.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG CCTCGCCACG GCCGGGGAAT CGAAACAGTC TTGAAGATTT ACAGCTGCAC 
GTGACAGTGT CCTGTGCCAA ACTGAAGCGG AAGAAGAACT GGTTTGGAAC GACTGTATAT 
ACAGAATTAG CTGTTGATGG TGAAACAAAG AAGACTGCAA AATCAAGCAG CTCGAGTAAC 
CCCAAATGGG AAGAACGGTT AACTGTGAGT GTGACCCCAC AAACTACACT TGAATTCAAG 
GTATGGAGCC ACCATTCGTG GAAAGCGGAT GCGTTGCTTG GCAAAACCGC TGTCGACTTA 
AAGCAAGCTC TGGAGACCCA TAACCGGAAG TTGGAAAGAG TGAAAGAACA ACTGAAACTT 
TCATTGGACG GCAAACATGG CACAGTACAG ACTGGCGAGC TGACAGTCAT TTTAGATGGA 
TTAAATGTGG AGCCTGAATC ACTTGCTACG TTTAACTCAA CAGCCACTCC AGCCAGAGAA 
ATCCAGCAGA ATGGGGATGC CGTCCGTGAG AATGGAGGAG AAACCTCTTT GGAAGCAAGA 
GTTGGAGCCA CTTGCGACAT GCCCAATGGG ACAGCTAGTG AAACCACATC CGAGCAGAAT 
GCCTTTAATA CACAAATAGC AAATATAGAT AGCACTCATT CCCCTTCTCG TGTTGCAGCC 
AGACCCAAAA ATACACCAGC ACCAAATCCA CTGACTGGCG ATAGAACTGT TAATGGCACA 
AGTGCTACAT CTGAAGCAAA TAGCACACCT TCCCATGAAG CTTTACCATC TCCTGCCGGA 
GCCCCCTCAT CAGATAGCAT AGAGATGATG GACAGTACAT CAGATGGGCC AGTATCAATA 
CCTTTAGATA GCGGCACGTC TGCAGAGAGC AGTTCTGCTG GGGCTTCATC AGAACCAGTA 
AGGGCTGCAG ACTCTGGGCC ATCACCTGCT GCTGGCAGCA ATAGCCCTGA TGCTGCAAAA 
CCAAGGCAGG CGTCATCTAC AGGTTCTGGG GATCCTGTCA GACAGCAGCC AAGCTCCGCT 
GCAGAGCCTT TGCCATCGGG ATGGGAACAG AGGAAAGACC CCCATGGTAG AACCTACTAC 
GTTGATCACA ACACAAGGAC TACAACATGG GAACGCCCAC AGCCTTTGCC TCCTGGGTGG 
GAGAGAAGGG TCGATGATCG TGGGAGAGTA TATTATGTTG ACCATAACAC AAGAACCACA 
ACATGGCAGA GGCCTACTAT GGAGTCTGTA CGAAACTTTG AACAGTGGCA GTCTCAGCGC 
AATCAACTCC AGGGAGCTAT GCAGCAGTTT AACCAGCGAT ACCTCTATTC GGCATCAATG 
TTATCCGCTG AAAATGACCC GTTAGGACCA CTTCCGCCAG GATGGGAGAG AAGAGTGGAT 
TCCACTGATC GTGTCTATTT TGTGAACCAC AATACAAAAA CAACACAGTG GGAGGACCCT 
CGAACTCAAG GTTTACAGAA CGAAGATCCA TTGCCAGAGG GCTGGGAAAT CCGCTACACA 
AGAGAAGGAG TAAAGTACTT TGTAGATCAC AATACAAAGA CGACTACTTT CAGCGACCCA 
AGAACTGGGA AGTCAGCAGT AAGCAAAGGG CCACAGATTG CCTATGAACG TAGTTTCCGC 
TGGAAGCTTG CTCATTTTCG TTATTTATGC CAGTCCAATG CACTGCCCAG CCATGTGAAG 
ATAACGGTGT CCAGGCAGAC GCTCTTTGAA GACTCTTTCC AGCAGATTAT GGCATTAAAA 
CCATATGATC TGAGAAGGCG ATTGTATGTC ATGTTCAGAG GGGAAGAAGG TTTGGACTAT 
GGAGGCTTAG CCAGAGAGTG GTTTTTCCTG CTTTCCCATG AGGTACTGAA CCCCATGTAC 
TGCCTGTTCG AGTATGCAGG CAAGAGCAAT TACTGCTTGC AGATCAACCC TGCCTCCACC 
ATTAATCCTG ACCACCTCTC CTATTTCTGC TTTATTGGCC GCTTTATTGC AATGGCGCTG 
TTCCATGGGA AGTTTATTGA TACTGGCTTC TCTCTTCCAT TCTACAAACG AATGCTCAGC 
AAGAAACTTA CCATTAAAGA TCTGGAATCG ATTGATCCCG AGTTCTACAA CTCTTTGATA 
TGGATACGGG ACAATAACAT TGAAGAATGT AACCTGGAAA TGTACTTTTC TGTGGACATG 
GAAATATTGG GGAAAGTCAC CTCACATGAT CTGAAGCCTG AAGGTTCCAA CATCCTTGTG 
ACTGAGGAGA ACAAGGAAGA ATATATTGGT CTCATGGCAG AATGGAGATT CTCACGTGGA 
GTTGAGGAAC AAACCAAAGC ATTTCTTGAT GGCTTTAATG CAGTGGTCCC ACTTCAGTGG 
CTACAGTACT TTGATGAAAA AGAGTTAGAG GTTATGCTGT GTGGCATGCA GGAGGTGGAT 
TTATCAGACT GGCAGCGGAA TACAGTCTAT CGGCATTACA CAAGGAATAG CAAGCAGATC 
ATATGGTTCT GGCAGTTTGT GAAGGAAATG GACAATGAGG TGCGGTTGCG GCTGCTGCAG 
TTTGTCACTG GTACCTGCAG GCTGCCGCTG GGAGGATTCG CAGAGCTCAT GGGAAGCAAC 
GGTCCACAGA AATTCTGCAT TGAGAAGGTT GGAAAAGAAA CATGGCTCCC TCGAAGTCAT 
ACATGTTTCA ATCGACTGGA CCTGCCGCCA TATAAAAGCT ATGAACAACT GAAGGAGAAG 
CTGCTTTTTG CAATTGAAGA AACGGAAGGA TTTGGCCAAG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819605.2
CDS	426..3170
Translation	MATASPRPGNRNSLEDLQLHVTVSCAKLKRKKNWFGTTVYTELAVDGETKKTAKSSSSSNPKWEERLTVSVTPQTTLEFKVWSHHSWKADALLGKTAVDLKQALETHNRKLERVKEQLKLSLDGKHGTVQTGELTVILDGLNVEPESLATFNSTATPAREIQQNGDAVRENGGETSLEARVGATCDMPNGTASETTSEQNAFNTQIANIDSTHSPSRVAARPKNTPAPNPLTGDRTVNGTSATSEANSTPSHEALPSPAGAPSSDSIEMMDSTSDGPVSIPLDSGTSAESSSAGASSEPVRAADSGPSPAAGSNSPDAAKPRQASSTGSGDPVRQQPSSAAEPLPSGWEQRKDPHGRTYYVDHNTRTTTWERPQPLPPGWERRVDDRGRVYYVDHNTRTTTWQRPTMESVRNFEQWQSQRNQLQGAMQQFNQRYLYSASMLSAENDPLGPLPPGWERRVDSTDRVYFVNHNTKTTQWEDPRTQGLQNEDPLPEGWEIRYTREGVKYFVDHNTKTTTFSDPRTGKSAVSKGPQIAYERSFRWKLAHFRYLCQSNALPSHVKITVSRQTLFEDSFQQIMALKPYDLRRRLYVMFRGEEGLDYGGLAREWFFLLSHEVLNPMYCLFEYAGKSNYCLQINPASTINPDHLSYFCFIGRFIAMALFHGKFIDTGFSLPFYKRMLSKKLTIKDLESIDPEFYNSLIWIRDNNIEECNLEMYFSVDMEILGKVTSHDLKPEGSNILVTEENKEEYIGLMAEWRFSRGVEEQTKAFLDGFNAVVPLQWLQYFDEKELEVMLCGMQEVDLSDWQRNTVYRHYTRNSKQIIWFWQFVKEMDNEVRLRLLQFVTGTCRLPLGGFAELMGSNGPQKFCIEKVGKETWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	XM_012964151.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis WW domain containing E3 ubiquitin protein ligase 1 (wwp1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012964151.3

ORF Insert Sequence:

ATGGCCACAG CCTCGCCACG GCCGGGGAAT CGAAACAGTC TTGAAGATTT ACAGCTGCAC 
GTGACAGTGT CCTGTGCCAA ACTGAAGCGG AAGAAGAACT GGTTTGGAAC GACTGTATAT 
ACAGAATTAG CTGTTGATGG TGAAACAAAG AAGACTGCAA AATCAAGCAG CTCGAGTAAC 
CCCAAATGGG AAGAACGGTT AACTGTGAGT GTGACCCCAC AAACTACACT TGAATTCAAG 
GTATGGAGCC ACCATTCGTG GAAAGCGGAT GCGTTGCTTG GCAAAACCGC TGTCGACTTA 
AAGCAAGCTC TGGAGACCCA TAACCGGAAG TTGGAAAGAG TGAAAGAACA ACTGAAACTT 
TCATTGGACG GCAAACATGG CACAGTACAG ACTGGCGAGC TGACAGTCAT TTTAGATGGA 
TTAAATGTGG AGCCTGAATC ACTTGCTACG TTTAACTCAA CAGCCACTCC AGCCAGAGAA 
ATCCAGCAGA ATGGGGATGC CGTCCGTGAG AATGGAGGAG AAACCTCTTT GGAAGCAAGA 
GTTGGAGCCA CTTGCGACAT GCCCAATGGG ACAGCTAGTG AAACCACATC CGAGCAGAAT 
GCCTTTAATA CACAAATAGC AAATATAGAT AGCACTCATT CCCCTTCTCG TGTTGCAGCC 
AGACCCAAAA ATACACCAGC ACCAAATCCA CTGACTGGCG ATAGAACTGT TAATGGCACA 
AGTGCTACAT CTGAAGCAAA TAGCACACCT TCCCATGAAG CTTTACCATC TCCTGCCGGA 
GCCCCCTCAT CAGATAGCAT AGAGATGATG GACAGTACAT CAGATGGGCC AGTATCAATA 
CCTTTAGATA GCGGCACGTC TGCAGAGAGC AGTTCTGCTG GGGCTTCATC AGAACCAGTA 
AGGGCTGCAG ACTCTGGGCC ATCACCTGCT GCTGGCAGCA ATAGCCCTGA TGCTGCAAAA 
CCAAGGCAGG CGTCATCTAC AGGTTCTGGG GATCCTGTCA GACAGCAGCC AAGCTCCGCT 
GCAGAGCCTT TGCCATCGGG ATGGGAACAG AGGAAAGACC CCCATGGTAG AACCTACTAC 
GTTGATCACA ACACAAGGAC TACAACATGG GAACGCCCAC AGCCTTTGCC TCCTGGGTGG 
GAGAGAAGGG TCGATGATCG TGGGAGAGTA TATTATGTTG ACCATAACAC AAGAACCACA 
ACATGGCAGA GGCCTACTAT GGAGTCTGTA CGAAACTTTG AACAGTGGCA GTCTCAGCGC 
AATCAACTCC AGGGAGCTAT GCAGCAGTTT AACCAGCGAT ACCTCTATTC GGCATCAATG 
TTATCCGCTG AAAATGACCC GTTAGGACCA CTTCCGCCAG GATGGGAGAG AAGAGTGGAT 
TCCACTGATC GTGTCTATTT TGTGAACCAC AATACAAAAA CAACACAGTG GGAGGACCCT 
CGAACTCAAG GTTTACAGAA CGAAGATCCA TTGCCAGAGG GCTGGGAAAT CCGCTACACA 
AGAGAAGGAG TAAAGTACTT TGTAGATCAC AATACAAAGA CGACTACTTT CAGCGACCCA 
AGAACTGGGA AGTCAGCAGT AAGCAAAGGG CCACAGATTG CCTATGAACG TAGTTTCCGC 
TGGAAGCTTG CTCATTTTCG TTATTTATGC CAGTCCAATG CACTGCCCAG CCATGTGAAG 
ATAACGGTGT CCAGGCAGAC GCTCTTTGAA GACTCTTTCC AGCAGATTAT GGCATTAAAA 
CCATATGATC TGAGAAGGCG ATTGTATGTC ATGTTCAGAG GGGAAGAAGG TTTGGACTAT 
GGAGGCTTAG CCAGAGAGTG GTTTTTCCTG CTTTCCCATG AGGTACTGAA CCCCATGTAC 
TGCCTGTTCG AGTATGCAGG CAAGAGCAAT TACTGCTTGC AGATCAACCC TGCCTCCACC 
ATTAATCCTG ACCACCTCTC CTATTTCTGC TTTATTGGCC GCTTTATTGC AATGGCGCTG 
TTCCATGGGA AGTTTATTGA TACTGGCTTC TCTCTTCCAT TCTACAAACG AATGCTCAGC 
AAGAAACTTA CCATTAAAGA TCTGGAATCG ATTGATCCCG AGTTCTACAA CTCTTTGATA 
TGGATACGGG ACAATAACAT TGAAGAATGT AACCTGGAAA TGTACTTTTC TGTGGACATG 
GAAATATTGG GGAAAGTCAC CTCACATGAT CTGAAGCCTG AAGGTTCCAA CATCCTTGTG 
ACTGAGGAGA ACAAGGAAGA ATATATTGGT CTCATGGCAG AATGGAGATT CTCACGTGGA 
GTTGAGGAAC AAACCAAAGC ATTTCTTGAT GGCTTTAATG CAGTGGTCCC ACTTCAGTGG 
CTACAGTACT TTGATGAAAA AGAGTTAGAG GTTATGCTGT GTGGCATGCA GGAGGTGGAT 
TTATCAGACT GGCAGCGGAA TACAGTCTAT CGGCATTACA CAAGGAATAG CAAGCAGATC 
ATATGGTTCT GGCAGTTTGT GAAGGAAATG GACAATGAGG TGCGGTTGCG GCTGCTGCAG 
TTTGTCACTG GTACCTGCAG GCTGCCGCTG GGAGGATTCG CAGAGCTCAT GGGAAGCAAC 
GGTCCACAGA AATTCTGCAT TGAGAAGGTT GGAAAAGAAA CATGGCTCCC TCGAAGTCAT 
ACATGTTTCA ATCGACTGGA CCTGCCGCCA TATAAAAGCT ATGAACAACT GAAGGAGAAG 
CTGCTTTTTG CAATTGAAGA AACGGAAGGA TTTGGCCAAG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wwp1 ( XM_012964150.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819604.2 Xenopus tropicalis WW domain containing E3 ubiquitin protein ligase 1 (wwp1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa24470
Clone ID Related Accession (Same CDS sequence)	NM_001097375.1 , XM_012964151.3 , XM_012964150.3 , XM_012964152.3
Accession Version	XM_012964150.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	NEDD4-like E3 ubiquitin-protein ligase WWP1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012964150.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG CCTCGCCACG GCCGGGGAAT CGAAACAGTC TTGAAGATTT ACAGCTGCAC 
GTGACAGTGT CCTGTGCCAA ACTGAAGCGG AAGAAGAACT GGTTTGGAAC GACTGTATAT 
ACAGAATTAG CTGTTGATGG TGAAACAAAG AAGACTGCAA AATCAAGCAG CTCGAGTAAC 
CCCAAATGGG AAGAACGGTT AACTGTGAGT GTGACCCCAC AAACTACACT TGAATTCAAG 
GTATGGAGCC ACCATTCGTG GAAAGCGGAT GCGTTGCTTG GCAAAACCGC TGTCGACTTA 
AAGCAAGCTC TGGAGACCCA TAACCGGAAG TTGGAAAGAG TGAAAGAACA ACTGAAACTT 
TCATTGGACG GCAAACATGG CACAGTACAG ACTGGCGAGC TGACAGTCAT TTTAGATGGA 
TTAAATGTGG AGCCTGAATC ACTTGCTACG TTTAACTCAA CAGCCACTCC AGCCAGAGAA 
ATCCAGCAGA ATGGGGATGC CGTCCGTGAG AATGGAGGAG AAACCTCTTT GGAAGCAAGA 
GTTGGAGCCA CTTGCGACAT GCCCAATGGG ACAGCTAGTG AAACCACATC CGAGCAGAAT 
GCCTTTAATA CACAAATAGC AAATATAGAT AGCACTCATT CCCCTTCTCG TGTTGCAGCC 
AGACCCAAAA ATACACCAGC ACCAAATCCA CTGACTGGCG ATAGAACTGT TAATGGCACA 
AGTGCTACAT CTGAAGCAAA TAGCACACCT TCCCATGAAG CTTTACCATC TCCTGCCGGA 
GCCCCCTCAT CAGATAGCAT AGAGATGATG GACAGTACAT CAGATGGGCC AGTATCAATA 
CCTTTAGATA GCGGCACGTC TGCAGAGAGC AGTTCTGCTG GGGCTTCATC AGAACCAGTA 
AGGGCTGCAG ACTCTGGGCC ATCACCTGCT GCTGGCAGCA ATAGCCCTGA TGCTGCAAAA 
CCAAGGCAGG CGTCATCTAC AGGTTCTGGG GATCCTGTCA GACAGCAGCC AAGCTCCGCT 
GCAGAGCCTT TGCCATCGGG ATGGGAACAG AGGAAAGACC CCCATGGTAG AACCTACTAC 
GTTGATCACA ACACAAGGAC TACAACATGG GAACGCCCAC AGCCTTTGCC TCCTGGGTGG 
GAGAGAAGGG TCGATGATCG TGGGAGAGTA TATTATGTTG ACCATAACAC AAGAACCACA 
ACATGGCAGA GGCCTACTAT GGAGTCTGTA CGAAACTTTG AACAGTGGCA GTCTCAGCGC 
AATCAACTCC AGGGAGCTAT GCAGCAGTTT AACCAGCGAT ACCTCTATTC GGCATCAATG 
TTATCCGCTG AAAATGACCC GTTAGGACCA CTTCCGCCAG GATGGGAGAG AAGAGTGGAT 
TCCACTGATC GTGTCTATTT TGTGAACCAC AATACAAAAA CAACACAGTG GGAGGACCCT 
CGAACTCAAG GTTTACAGAA CGAAGATCCA TTGCCAGAGG GCTGGGAAAT CCGCTACACA 
AGAGAAGGAG TAAAGTACTT TGTAGATCAC AATACAAAGA CGACTACTTT CAGCGACCCA 
AGAACTGGGA AGTCAGCAGT AAGCAAAGGG CCACAGATTG CCTATGAACG TAGTTTCCGC 
TGGAAGCTTG CTCATTTTCG TTATTTATGC CAGTCCAATG CACTGCCCAG CCATGTGAAG 
ATAACGGTGT CCAGGCAGAC GCTCTTTGAA GACTCTTTCC AGCAGATTAT GGCATTAAAA 
CCATATGATC TGAGAAGGCG ATTGTATGTC ATGTTCAGAG GGGAAGAAGG TTTGGACTAT 
GGAGGCTTAG CCAGAGAGTG GTTTTTCCTG CTTTCCCATG AGGTACTGAA CCCCATGTAC 
TGCCTGTTCG AGTATGCAGG CAAGAGCAAT TACTGCTTGC AGATCAACCC TGCCTCCACC 
ATTAATCCTG ACCACCTCTC CTATTTCTGC TTTATTGGCC GCTTTATTGC AATGGCGCTG 
TTCCATGGGA AGTTTATTGA TACTGGCTTC TCTCTTCCAT TCTACAAACG AATGCTCAGC 
AAGAAACTTA CCATTAAAGA TCTGGAATCG ATTGATCCCG AGTTCTACAA CTCTTTGATA 
TGGATACGGG ACAATAACAT TGAAGAATGT AACCTGGAAA TGTACTTTTC TGTGGACATG 
GAAATATTGG GGAAAGTCAC CTCACATGAT CTGAAGCCTG AAGGTTCCAA CATCCTTGTG 
ACTGAGGAGA ACAAGGAAGA ATATATTGGT CTCATGGCAG AATGGAGATT CTCACGTGGA 
GTTGAGGAAC AAACCAAAGC ATTTCTTGAT GGCTTTAATG CAGTGGTCCC ACTTCAGTGG 
CTACAGTACT TTGATGAAAA AGAGTTAGAG GTTATGCTGT GTGGCATGCA GGAGGTGGAT 
TTATCAGACT GGCAGCGGAA TACAGTCTAT CGGCATTACA CAAGGAATAG CAAGCAGATC 
ATATGGTTCT GGCAGTTTGT GAAGGAAATG GACAATGAGG TGCGGTTGCG GCTGCTGCAG 
TTTGTCACTG GTACCTGCAG GCTGCCGCTG GGAGGATTCG CAGAGCTCAT GGGAAGCAAC 
GGTCCACAGA AATTCTGCAT TGAGAAGGTT GGAAAAGAAA CATGGCTCCC TCGAAGTCAT 
ACATGTTTCA ATCGACTGGA CCTGCCGCCA TATAAAAGCT ATGAACAACT GAAGGAGAAG 
CTGCTTTTTG CAATTGAAGA AACGGAAGGA TTTGGCCAAG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819604.2
CDS	353..3097
Translation	MATASPRPGNRNSLEDLQLHVTVSCAKLKRKKNWFGTTVYTELAVDGETKKTAKSSSSSNPKWEERLTVSVTPQTTLEFKVWSHHSWKADALLGKTAVDLKQALETHNRKLERVKEQLKLSLDGKHGTVQTGELTVILDGLNVEPESLATFNSTATPAREIQQNGDAVRENGGETSLEARVGATCDMPNGTASETTSEQNAFNTQIANIDSTHSPSRVAARPKNTPAPNPLTGDRTVNGTSATSEANSTPSHEALPSPAGAPSSDSIEMMDSTSDGPVSIPLDSGTSAESSSAGASSEPVRAADSGPSPAAGSNSPDAAKPRQASSTGSGDPVRQQPSSAAEPLPSGWEQRKDPHGRTYYVDHNTRTTTWERPQPLPPGWERRVDDRGRVYYVDHNTRTTTWQRPTMESVRNFEQWQSQRNQLQGAMQQFNQRYLYSASMLSAENDPLGPLPPGWERRVDSTDRVYFVNHNTKTTQWEDPRTQGLQNEDPLPEGWEIRYTREGVKYFVDHNTKTTTFSDPRTGKSAVSKGPQIAYERSFRWKLAHFRYLCQSNALPSHVKITVSRQTLFEDSFQQIMALKPYDLRRRLYVMFRGEEGLDYGGLAREWFFLLSHEVLNPMYCLFEYAGKSNYCLQINPASTINPDHLSYFCFIGRFIAMALFHGKFIDTGFSLPFYKRMLSKKLTIKDLESIDPEFYNSLIWIRDNNIEECNLEMYFSVDMEILGKVTSHDLKPEGSNILVTEENKEEYIGLMAEWRFSRGVEEQTKAFLDGFNAVVPLQWLQYFDEKELEVMLCGMQEVDLSDWQRNTVYRHYTRNSKQIIWFWQFVKEMDNEVRLRLLQFVTGTCRLPLGGFAELMGSNGPQKFCIEKVGKETWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	XM_012964150.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis WW domain containing E3 ubiquitin protein ligase 1 (wwp1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012964150.3

ORF Insert Sequence:

ATGGCCACAG CCTCGCCACG GCCGGGGAAT CGAAACAGTC TTGAAGATTT ACAGCTGCAC 
GTGACAGTGT CCTGTGCCAA ACTGAAGCGG AAGAAGAACT GGTTTGGAAC GACTGTATAT 
ACAGAATTAG CTGTTGATGG TGAAACAAAG AAGACTGCAA AATCAAGCAG CTCGAGTAAC 
CCCAAATGGG AAGAACGGTT AACTGTGAGT GTGACCCCAC AAACTACACT TGAATTCAAG 
GTATGGAGCC ACCATTCGTG GAAAGCGGAT GCGTTGCTTG GCAAAACCGC TGTCGACTTA 
AAGCAAGCTC TGGAGACCCA TAACCGGAAG TTGGAAAGAG TGAAAGAACA ACTGAAACTT 
TCATTGGACG GCAAACATGG CACAGTACAG ACTGGCGAGC TGACAGTCAT TTTAGATGGA 
TTAAATGTGG AGCCTGAATC ACTTGCTACG TTTAACTCAA CAGCCACTCC AGCCAGAGAA 
ATCCAGCAGA ATGGGGATGC CGTCCGTGAG AATGGAGGAG AAACCTCTTT GGAAGCAAGA 
GTTGGAGCCA CTTGCGACAT GCCCAATGGG ACAGCTAGTG AAACCACATC CGAGCAGAAT 
GCCTTTAATA CACAAATAGC AAATATAGAT AGCACTCATT CCCCTTCTCG TGTTGCAGCC 
AGACCCAAAA ATACACCAGC ACCAAATCCA CTGACTGGCG ATAGAACTGT TAATGGCACA 
AGTGCTACAT CTGAAGCAAA TAGCACACCT TCCCATGAAG CTTTACCATC TCCTGCCGGA 
GCCCCCTCAT CAGATAGCAT AGAGATGATG GACAGTACAT CAGATGGGCC AGTATCAATA 
CCTTTAGATA GCGGCACGTC TGCAGAGAGC AGTTCTGCTG GGGCTTCATC AGAACCAGTA 
AGGGCTGCAG ACTCTGGGCC ATCACCTGCT GCTGGCAGCA ATAGCCCTGA TGCTGCAAAA 
CCAAGGCAGG CGTCATCTAC AGGTTCTGGG GATCCTGTCA GACAGCAGCC AAGCTCCGCT 
GCAGAGCCTT TGCCATCGGG ATGGGAACAG AGGAAAGACC CCCATGGTAG AACCTACTAC 
GTTGATCACA ACACAAGGAC TACAACATGG GAACGCCCAC AGCCTTTGCC TCCTGGGTGG 
GAGAGAAGGG TCGATGATCG TGGGAGAGTA TATTATGTTG ACCATAACAC AAGAACCACA 
ACATGGCAGA GGCCTACTAT GGAGTCTGTA CGAAACTTTG AACAGTGGCA GTCTCAGCGC 
AATCAACTCC AGGGAGCTAT GCAGCAGTTT AACCAGCGAT ACCTCTATTC GGCATCAATG 
TTATCCGCTG AAAATGACCC GTTAGGACCA CTTCCGCCAG GATGGGAGAG AAGAGTGGAT 
TCCACTGATC GTGTCTATTT TGTGAACCAC AATACAAAAA CAACACAGTG GGAGGACCCT 
CGAACTCAAG GTTTACAGAA CGAAGATCCA TTGCCAGAGG GCTGGGAAAT CCGCTACACA 
AGAGAAGGAG TAAAGTACTT TGTAGATCAC AATACAAAGA CGACTACTTT CAGCGACCCA 
AGAACTGGGA AGTCAGCAGT AAGCAAAGGG CCACAGATTG CCTATGAACG TAGTTTCCGC 
TGGAAGCTTG CTCATTTTCG TTATTTATGC CAGTCCAATG CACTGCCCAG CCATGTGAAG 
ATAACGGTGT CCAGGCAGAC GCTCTTTGAA GACTCTTTCC AGCAGATTAT GGCATTAAAA 
CCATATGATC TGAGAAGGCG ATTGTATGTC ATGTTCAGAG GGGAAGAAGG TTTGGACTAT 
GGAGGCTTAG CCAGAGAGTG GTTTTTCCTG CTTTCCCATG AGGTACTGAA CCCCATGTAC 
TGCCTGTTCG AGTATGCAGG CAAGAGCAAT TACTGCTTGC AGATCAACCC TGCCTCCACC 
ATTAATCCTG ACCACCTCTC CTATTTCTGC TTTATTGGCC GCTTTATTGC AATGGCGCTG 
TTCCATGGGA AGTTTATTGA TACTGGCTTC TCTCTTCCAT TCTACAAACG AATGCTCAGC 
AAGAAACTTA CCATTAAAGA TCTGGAATCG ATTGATCCCG AGTTCTACAA CTCTTTGATA 
TGGATACGGG ACAATAACAT TGAAGAATGT AACCTGGAAA TGTACTTTTC TGTGGACATG 
GAAATATTGG GGAAAGTCAC CTCACATGAT CTGAAGCCTG AAGGTTCCAA CATCCTTGTG 
ACTGAGGAGA ACAAGGAAGA ATATATTGGT CTCATGGCAG AATGGAGATT CTCACGTGGA 
GTTGAGGAAC AAACCAAAGC ATTTCTTGAT GGCTTTAATG CAGTGGTCCC ACTTCAGTGG 
CTACAGTACT TTGATGAAAA AGAGTTAGAG GTTATGCTGT GTGGCATGCA GGAGGTGGAT 
TTATCAGACT GGCAGCGGAA TACAGTCTAT CGGCATTACA CAAGGAATAG CAAGCAGATC 
ATATGGTTCT GGCAGTTTGT GAAGGAAATG GACAATGAGG TGCGGTTGCG GCTGCTGCAG 
TTTGTCACTG GTACCTGCAG GCTGCCGCTG GGAGGATTCG CAGAGCTCAT GGGAAGCAAC 
GGTCCACAGA AATTCTGCAT TGAGAAGGTT GGAAAAGAAA CATGGCTCCC TCGAAGTCAT 
ACATGTTTCA ATCGACTGGA CCTGCCGCCA TATAAAAGCT ATGAACAACT GAAGGAGAAG 
CTGCTTTTTG CAATTGAAGA AACGGAAGGA TTTGGCCAAG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wwp1 ( XM_012964152.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819606.2 Xenopus tropicalis WW domain containing E3 ubiquitin protein ligase 1 (wwp1), transcript variant X3, mRNA.

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CloneID	OXa24470
Clone ID Related Accession (Same CDS sequence)	NM_001097375.1 , XM_012964151.3 , XM_012964150.3 , XM_012964152.3
Accession Version	XM_012964152.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	NEDD4-like E3 ubiquitin-protein ligase WWP1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012964152.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG CCTCGCCACG GCCGGGGAAT CGAAACAGTC TTGAAGATTT ACAGCTGCAC 
GTGACAGTGT CCTGTGCCAA ACTGAAGCGG AAGAAGAACT GGTTTGGAAC GACTGTATAT 
ACAGAATTAG CTGTTGATGG TGAAACAAAG AAGACTGCAA AATCAAGCAG CTCGAGTAAC 
CCCAAATGGG AAGAACGGTT AACTGTGAGT GTGACCCCAC AAACTACACT TGAATTCAAG 
GTATGGAGCC ACCATTCGTG GAAAGCGGAT GCGTTGCTTG GCAAAACCGC TGTCGACTTA 
AAGCAAGCTC TGGAGACCCA TAACCGGAAG TTGGAAAGAG TGAAAGAACA ACTGAAACTT 
TCATTGGACG GCAAACATGG CACAGTACAG ACTGGCGAGC TGACAGTCAT TTTAGATGGA 
TTAAATGTGG AGCCTGAATC ACTTGCTACG TTTAACTCAA CAGCCACTCC AGCCAGAGAA 
ATCCAGCAGA ATGGGGATGC CGTCCGTGAG AATGGAGGAG AAACCTCTTT GGAAGCAAGA 
GTTGGAGCCA CTTGCGACAT GCCCAATGGG ACAGCTAGTG AAACCACATC CGAGCAGAAT 
GCCTTTAATA CACAAATAGC AAATATAGAT AGCACTCATT CCCCTTCTCG TGTTGCAGCC 
AGACCCAAAA ATACACCAGC ACCAAATCCA CTGACTGGCG ATAGAACTGT TAATGGCACA 
AGTGCTACAT CTGAAGCAAA TAGCACACCT TCCCATGAAG CTTTACCATC TCCTGCCGGA 
GCCCCCTCAT CAGATAGCAT AGAGATGATG GACAGTACAT CAGATGGGCC AGTATCAATA 
CCTTTAGATA GCGGCACGTC TGCAGAGAGC AGTTCTGCTG GGGCTTCATC AGAACCAGTA 
AGGGCTGCAG ACTCTGGGCC ATCACCTGCT GCTGGCAGCA ATAGCCCTGA TGCTGCAAAA 
CCAAGGCAGG CGTCATCTAC AGGTTCTGGG GATCCTGTCA GACAGCAGCC AAGCTCCGCT 
GCAGAGCCTT TGCCATCGGG ATGGGAACAG AGGAAAGACC CCCATGGTAG AACCTACTAC 
GTTGATCACA ACACAAGGAC TACAACATGG GAACGCCCAC AGCCTTTGCC TCCTGGGTGG 
GAGAGAAGGG TCGATGATCG TGGGAGAGTA TATTATGTTG ACCATAACAC AAGAACCACA 
ACATGGCAGA GGCCTACTAT GGAGTCTGTA CGAAACTTTG AACAGTGGCA GTCTCAGCGC 
AATCAACTCC AGGGAGCTAT GCAGCAGTTT AACCAGCGAT ACCTCTATTC GGCATCAATG 
TTATCCGCTG AAAATGACCC GTTAGGACCA CTTCCGCCAG GATGGGAGAG AAGAGTGGAT 
TCCACTGATC GTGTCTATTT TGTGAACCAC AATACAAAAA CAACACAGTG GGAGGACCCT 
CGAACTCAAG GTTTACAGAA CGAAGATCCA TTGCCAGAGG GCTGGGAAAT CCGCTACACA 
AGAGAAGGAG TAAAGTACTT TGTAGATCAC AATACAAAGA CGACTACTTT CAGCGACCCA 
AGAACTGGGA AGTCAGCAGT AAGCAAAGGG CCACAGATTG CCTATGAACG TAGTTTCCGC 
TGGAAGCTTG CTCATTTTCG TTATTTATGC CAGTCCAATG CACTGCCCAG CCATGTGAAG 
ATAACGGTGT CCAGGCAGAC GCTCTTTGAA GACTCTTTCC AGCAGATTAT GGCATTAAAA 
CCATATGATC TGAGAAGGCG ATTGTATGTC ATGTTCAGAG GGGAAGAAGG TTTGGACTAT 
GGAGGCTTAG CCAGAGAGTG GTTTTTCCTG CTTTCCCATG AGGTACTGAA CCCCATGTAC 
TGCCTGTTCG AGTATGCAGG CAAGAGCAAT TACTGCTTGC AGATCAACCC TGCCTCCACC 
ATTAATCCTG ACCACCTCTC CTATTTCTGC TTTATTGGCC GCTTTATTGC AATGGCGCTG 
TTCCATGGGA AGTTTATTGA TACTGGCTTC TCTCTTCCAT TCTACAAACG AATGCTCAGC 
AAGAAACTTA CCATTAAAGA TCTGGAATCG ATTGATCCCG AGTTCTACAA CTCTTTGATA 
TGGATACGGG ACAATAACAT TGAAGAATGT AACCTGGAAA TGTACTTTTC TGTGGACATG 
GAAATATTGG GGAAAGTCAC CTCACATGAT CTGAAGCCTG AAGGTTCCAA CATCCTTGTG 
ACTGAGGAGA ACAAGGAAGA ATATATTGGT CTCATGGCAG AATGGAGATT CTCACGTGGA 
GTTGAGGAAC AAACCAAAGC ATTTCTTGAT GGCTTTAATG CAGTGGTCCC ACTTCAGTGG 
CTACAGTACT TTGATGAAAA AGAGTTAGAG GTTATGCTGT GTGGCATGCA GGAGGTGGAT 
TTATCAGACT GGCAGCGGAA TACAGTCTAT CGGCATTACA CAAGGAATAG CAAGCAGATC 
ATATGGTTCT GGCAGTTTGT GAAGGAAATG GACAATGAGG TGCGGTTGCG GCTGCTGCAG 
TTTGTCACTG GTACCTGCAG GCTGCCGCTG GGAGGATTCG CAGAGCTCAT GGGAAGCAAC 
GGTCCACAGA AATTCTGCAT TGAGAAGGTT GGAAAAGAAA CATGGCTCCC TCGAAGTCAT 
ACATGTTTCA ATCGACTGGA CCTGCCGCCA TATAAAAGCT ATGAACAACT GAAGGAGAAG 
CTGCTTTTTG CAATTGAAGA AACGGAAGGA TTTGGCCAAG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819606.2
CDS	41..2785
Translation	MATASPRPGNRNSLEDLQLHVTVSCAKLKRKKNWFGTTVYTELAVDGETKKTAKSSSSSNPKWEERLTVSVTPQTTLEFKVWSHHSWKADALLGKTAVDLKQALETHNRKLERVKEQLKLSLDGKHGTVQTGELTVILDGLNVEPESLATFNSTATPAREIQQNGDAVRENGGETSLEARVGATCDMPNGTASETTSEQNAFNTQIANIDSTHSPSRVAARPKNTPAPNPLTGDRTVNGTSATSEANSTPSHEALPSPAGAPSSDSIEMMDSTSDGPVSIPLDSGTSAESSSAGASSEPVRAADSGPSPAAGSNSPDAAKPRQASSTGSGDPVRQQPSSAAEPLPSGWEQRKDPHGRTYYVDHNTRTTTWERPQPLPPGWERRVDDRGRVYYVDHNTRTTTWQRPTMESVRNFEQWQSQRNQLQGAMQQFNQRYLYSASMLSAENDPLGPLPPGWERRVDSTDRVYFVNHNTKTTQWEDPRTQGLQNEDPLPEGWEIRYTREGVKYFVDHNTKTTTFSDPRTGKSAVSKGPQIAYERSFRWKLAHFRYLCQSNALPSHVKITVSRQTLFEDSFQQIMALKPYDLRRRLYVMFRGEEGLDYGGLAREWFFLLSHEVLNPMYCLFEYAGKSNYCLQINPASTINPDHLSYFCFIGRFIAMALFHGKFIDTGFSLPFYKRMLSKKLTIKDLESIDPEFYNSLIWIRDNNIEECNLEMYFSVDMEILGKVTSHDLKPEGSNILVTEENKEEYIGLMAEWRFSRGVEEQTKAFLDGFNAVVPLQWLQYFDEKELEVMLCGMQEVDLSDWQRNTVYRHYTRNSKQIIWFWQFVKEMDNEVRLRLLQFVTGTCRLPLGGFAELMGSNGPQKFCIEKVGKETWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	XM_012964152.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis WW domain containing E3 ubiquitin protein ligase 1 (wwp1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012964152.3

ORF Insert Sequence:

ATGGCCACAG CCTCGCCACG GCCGGGGAAT CGAAACAGTC TTGAAGATTT ACAGCTGCAC 
GTGACAGTGT CCTGTGCCAA ACTGAAGCGG AAGAAGAACT GGTTTGGAAC GACTGTATAT 
ACAGAATTAG CTGTTGATGG TGAAACAAAG AAGACTGCAA AATCAAGCAG CTCGAGTAAC 
CCCAAATGGG AAGAACGGTT AACTGTGAGT GTGACCCCAC AAACTACACT TGAATTCAAG 
GTATGGAGCC ACCATTCGTG GAAAGCGGAT GCGTTGCTTG GCAAAACCGC TGTCGACTTA 
AAGCAAGCTC TGGAGACCCA TAACCGGAAG TTGGAAAGAG TGAAAGAACA ACTGAAACTT 
TCATTGGACG GCAAACATGG CACAGTACAG ACTGGCGAGC TGACAGTCAT TTTAGATGGA 
TTAAATGTGG AGCCTGAATC ACTTGCTACG TTTAACTCAA CAGCCACTCC AGCCAGAGAA 
ATCCAGCAGA ATGGGGATGC CGTCCGTGAG AATGGAGGAG AAACCTCTTT GGAAGCAAGA 
GTTGGAGCCA CTTGCGACAT GCCCAATGGG ACAGCTAGTG AAACCACATC CGAGCAGAAT 
GCCTTTAATA CACAAATAGC AAATATAGAT AGCACTCATT CCCCTTCTCG TGTTGCAGCC 
AGACCCAAAA ATACACCAGC ACCAAATCCA CTGACTGGCG ATAGAACTGT TAATGGCACA 
AGTGCTACAT CTGAAGCAAA TAGCACACCT TCCCATGAAG CTTTACCATC TCCTGCCGGA 
GCCCCCTCAT CAGATAGCAT AGAGATGATG GACAGTACAT CAGATGGGCC AGTATCAATA 
CCTTTAGATA GCGGCACGTC TGCAGAGAGC AGTTCTGCTG GGGCTTCATC AGAACCAGTA 
AGGGCTGCAG ACTCTGGGCC ATCACCTGCT GCTGGCAGCA ATAGCCCTGA TGCTGCAAAA 
CCAAGGCAGG CGTCATCTAC AGGTTCTGGG GATCCTGTCA GACAGCAGCC AAGCTCCGCT 
GCAGAGCCTT TGCCATCGGG ATGGGAACAG AGGAAAGACC CCCATGGTAG AACCTACTAC 
GTTGATCACA ACACAAGGAC TACAACATGG GAACGCCCAC AGCCTTTGCC TCCTGGGTGG 
GAGAGAAGGG TCGATGATCG TGGGAGAGTA TATTATGTTG ACCATAACAC AAGAACCACA 
ACATGGCAGA GGCCTACTAT GGAGTCTGTA CGAAACTTTG AACAGTGGCA GTCTCAGCGC 
AATCAACTCC AGGGAGCTAT GCAGCAGTTT AACCAGCGAT ACCTCTATTC GGCATCAATG 
TTATCCGCTG AAAATGACCC GTTAGGACCA CTTCCGCCAG GATGGGAGAG AAGAGTGGAT 
TCCACTGATC GTGTCTATTT TGTGAACCAC AATACAAAAA CAACACAGTG GGAGGACCCT 
CGAACTCAAG GTTTACAGAA CGAAGATCCA TTGCCAGAGG GCTGGGAAAT CCGCTACACA 
AGAGAAGGAG TAAAGTACTT TGTAGATCAC AATACAAAGA CGACTACTTT CAGCGACCCA 
AGAACTGGGA AGTCAGCAGT AAGCAAAGGG CCACAGATTG CCTATGAACG TAGTTTCCGC 
TGGAAGCTTG CTCATTTTCG TTATTTATGC CAGTCCAATG CACTGCCCAG CCATGTGAAG 
ATAACGGTGT CCAGGCAGAC GCTCTTTGAA GACTCTTTCC AGCAGATTAT GGCATTAAAA 
CCATATGATC TGAGAAGGCG ATTGTATGTC ATGTTCAGAG GGGAAGAAGG TTTGGACTAT 
GGAGGCTTAG CCAGAGAGTG GTTTTTCCTG CTTTCCCATG AGGTACTGAA CCCCATGTAC 
TGCCTGTTCG AGTATGCAGG CAAGAGCAAT TACTGCTTGC AGATCAACCC TGCCTCCACC 
ATTAATCCTG ACCACCTCTC CTATTTCTGC TTTATTGGCC GCTTTATTGC AATGGCGCTG 
TTCCATGGGA AGTTTATTGA TACTGGCTTC TCTCTTCCAT TCTACAAACG AATGCTCAGC 
AAGAAACTTA CCATTAAAGA TCTGGAATCG ATTGATCCCG AGTTCTACAA CTCTTTGATA 
TGGATACGGG ACAATAACAT TGAAGAATGT AACCTGGAAA TGTACTTTTC TGTGGACATG 
GAAATATTGG GGAAAGTCAC CTCACATGAT CTGAAGCCTG AAGGTTCCAA CATCCTTGTG 
ACTGAGGAGA ACAAGGAAGA ATATATTGGT CTCATGGCAG AATGGAGATT CTCACGTGGA 
GTTGAGGAAC AAACCAAAGC ATTTCTTGAT GGCTTTAATG CAGTGGTCCC ACTTCAGTGG 
CTACAGTACT TTGATGAAAA AGAGTTAGAG GTTATGCTGT GTGGCATGCA GGAGGTGGAT 
TTATCAGACT GGCAGCGGAA TACAGTCTAT CGGCATTACA CAAGGAATAG CAAGCAGATC 
ATATGGTTCT GGCAGTTTGT GAAGGAAATG GACAATGAGG TGCGGTTGCG GCTGCTGCAG 
TTTGTCACTG GTACCTGCAG GCTGCCGCTG GGAGGATTCG CAGAGCTCAT GGGAAGCAAC 
GGTCCACAGA AATTCTGCAT TGAGAAGGTT GGAAAAGAAA CATGGCTCCC TCGAAGTCAT 
ACATGTTTCA ATCGACTGGA CCTGCCGCCA TATAAAAGCT ATGAACAACT GAAGGAGAAG 
CTGCTTTTTG CAATTGAAGA AACGGAAGGA TTTGGCCAAG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wwp1 ( XM_018094505.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949994.1 Xenopus tropicalis WW domain containing E3 ubiquitin protein ligase 1 (wwp1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa44834
Clone ID Related Accession (Same CDS sequence)	XM_018094505.1
Accession Version	XM_018094505.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1644bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	NEDD4-like E3 ubiquitin-protein ligase WWP1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAACGC CCACAGCCTT TGCCTCCTGG GTAAGGTGGG AGAGAAGGGT CGATGATCGT 
GGGAGAGTAT ATTATGTTGA CCATAACACA AGAACCACAA CATGGCAGAG GCCTACTATG 
GAGTCTGTAC GAAACTTTGA ACAGTGGCAG TCTCAGCGCA ATCAACTCCA GGGAGCTATG 
CAGCAGTTTA ACCAGCGATA CCTCTATTCG GCATCAATGT TATCCGCTGA AAATGACCCG 
TTAGGACCAC TTCCGCCAGG ATGGGAGAGA AGAGTGGATT CCACTGATCG TGTCTATTTT 
GTGAACCACA ATACAAAAAC AACACAGTGG GAGGACCCTC GAACTCAAGG TTTACAGAAC 
GAAGATCCAT TGCCAGAGGG CTGGGAAATC CGCTACACAA GAGAAGGAGT AAAGTACTTT 
GTAGATCACA ATACAAAGAC GACTACTTTC AGCGACCCAA GAACTGGGAA GTCAGCAGTA 
AGCAAAGGGC CACAGATTGC CTATGAACGT AGTTTCCGCT GGAAGCTTGC TCATTTTCGT 
TATTTATGCC AGTCCAATGC ACTGCCCAGC CATGTGAAGA TAACGGTGTC CAGGCAGACG 
CTCTTTGAAG ACTCTTTCCA GCAGATTATG GCATTAAAAC CATATGATCT GAGAAGGCGA 
TTGTATGTCA TGTTCAGAGG GGAAGAAGGT CTGGACTATG GAGGCTTAGC CAGAGAGTGG 
TTTTTCCTGC TTTCCCATGA GGTACTGAAC CCCATGTACT GCCTGTTCGA GTATGCAGGC 
AAGAGCAATT ACTGCTTGCA GATCAACCCT GCCTCCACCA TTAATCCTGA CCACCTCTCC 
TATTTCTGCT TTATTGGCCG CTTTATTGCA ATGGCGCTGT TCCATGGGAA GTTTATTGAT 
ACTGGCTTCT CTCTTCCATT CTACAAACGA ATGCTCAGCA AGAAACTTAC CATTAAAGAT 
CTGGAATCGA TTGATCCCGA GTTCTACAAC TCTTTGATAT GGATACGGGA CAATAACATT 
GAAGAATGTA ACCTGGAAAT GTACTTTTCT GTGGACATGG AAATATTGGG GAAAGTCACC 
TCACATGATC TGAAGCCTGA AGGTTCCAAC ATCCTTGTGA CTGAGGAGAA CAAGGAAGAA 
TATATTGGTC TCATGGCAGA ATGGAGATTC TCACGTGGAG TTGAAGAACA AACCAAAGCA 
TTTCTTGATG GCTTTAATGC AGTGGTCCCA CTTCAGTGGC TACAGTACTT TGATGAAAAA 
GAGTTAGAGG TTATGCTGTG TGGCATGCAG GAGGTGGATT TATCAGACTG GCAGCGGAAT 
ACAGTCTATC GGCATTACAC AAGGAATAGC AAGCAGATCA TATGGTTCTG GCAGTTTGTG 
AAGGAAATGG ACAATGAGGT GCGGTTGCGG CTGCTGCAGT TTGTCACTGG TACCTGCAGG 
CTGCCGCTGG GAGGATTCGC AGAGCTCATG GGAAGCAACG GTCCACAGAA ATTCTGCATT 
GAGAAGGTTG GAAAAGAAAC ATGGCTCCCT CGAAGTCATA CATGTTTCAA TCGACTGGAC 
CTGCCGCCAT ATAAAAGCTA TGAACAACTG AAGGAGAAGC TGCTTTTTGC AATTGAAGAA 
ACGGAAGGAT TTGGCCAAGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949994.1
CDS	157..1800
Translation	MGTPTAFASWVRWERRVDDRGRVYYVDHNTRTTTWQRPTMESVRNFEQWQSQRNQLQGAMQQFNQRYLYSASMLSAENDPLGPLPPGWERRVDSTDRVYFVNHNTKTTQWEDPRTQGLQNEDPLPEGWEIRYTREGVKYFVDHNTKTTTFSDPRTGKSAVSKGPQIAYERSFRWKLAHFRYLCQSNALPSHVKITVSRQTLFEDSFQQIMALKPYDLRRRLYVMFRGEEGLDYGGLAREWFFLLSHEVLNPMYCLFEYAGKSNYCLQINPASTINPDHLSYFCFIGRFIAMALFHGKFIDTGFSLPFYKRMLSKKLTIKDLESIDPEFYNSLIWIRDNNIEECNLEMYFSVDMEILGKVTSHDLKPEGSNILVTEENKEEYIGLMAEWRFSRGVEEQTKAFLDGFNAVVPLQWLQYFDEKELEVMLCGMQEVDLSDWQRNTVYRHYTRNSKQIIWFWQFVKEMDNEVRLRLLQFVTGTCRLPLGGFAELMGSNGPQKFCIEKVGKETWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	XM_018094505.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis WW domain containing E3 ubiquitin protein ligase 1 (wwp1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094505.1

ORF Insert Sequence:

ATGGGAACGC CCACAGCCTT TGCCTCCTGG GTAAGGTGGG AGAGAAGGGT CGATGATCGT 
GGGAGAGTAT ATTATGTTGA CCATAACACA AGAACCACAA CATGGCAGAG GCCTACTATG 
GAGTCTGTAC GAAACTTTGA ACAGTGGCAG TCTCAGCGCA ATCAACTCCA GGGAGCTATG 
CAGCAGTTTA ACCAGCGATA CCTCTATTCG GCATCAATGT TATCCGCTGA AAATGACCCG 
TTAGGACCAC TTCCGCCAGG ATGGGAGAGA AGAGTGGATT CCACTGATCG TGTCTATTTT 
GTGAACCACA ATACAAAAAC AACACAGTGG GAGGACCCTC GAACTCAAGG TTTACAGAAC 
GAAGATCCAT TGCCAGAGGG CTGGGAAATC CGCTACACAA GAGAAGGAGT AAAGTACTTT 
GTAGATCACA ATACAAAGAC GACTACTTTC AGCGACCCAA GAACTGGGAA GTCAGCAGTA 
AGCAAAGGGC CACAGATTGC CTATGAACGT AGTTTCCGCT GGAAGCTTGC TCATTTTCGT 
TATTTATGCC AGTCCAATGC ACTGCCCAGC CATGTGAAGA TAACGGTGTC CAGGCAGACG 
CTCTTTGAAG ACTCTTTCCA GCAGATTATG GCATTAAAAC CATATGATCT GAGAAGGCGA 
TTGTATGTCA TGTTCAGAGG GGAAGAAGGT CTGGACTATG GAGGCTTAGC CAGAGAGTGG 
TTTTTCCTGC TTTCCCATGA GGTACTGAAC CCCATGTACT GCCTGTTCGA GTATGCAGGC 
AAGAGCAATT ACTGCTTGCA GATCAACCCT GCCTCCACCA TTAATCCTGA CCACCTCTCC 
TATTTCTGCT TTATTGGCCG CTTTATTGCA ATGGCGCTGT TCCATGGGAA GTTTATTGAT 
ACTGGCTTCT CTCTTCCATT CTACAAACGA ATGCTCAGCA AGAAACTTAC CATTAAAGAT 
CTGGAATCGA TTGATCCCGA GTTCTACAAC TCTTTGATAT GGATACGGGA CAATAACATT 
GAAGAATGTA ACCTGGAAAT GTACTTTTCT GTGGACATGG AAATATTGGG GAAAGTCACC 
TCACATGATC TGAAGCCTGA AGGTTCCAAC ATCCTTGTGA CTGAGGAGAA CAAGGAAGAA 
TATATTGGTC TCATGGCAGA ATGGAGATTC TCACGTGGAG TTGAAGAACA AACCAAAGCA 
TTTCTTGATG GCTTTAATGC AGTGGTCCCA CTTCAGTGGC TACAGTACTT TGATGAAAAA 
GAGTTAGAGG TTATGCTGTG TGGCATGCAG GAGGTGGATT TATCAGACTG GCAGCGGAAT 
ACAGTCTATC GGCATTACAC AAGGAATAGC AAGCAGATCA TATGGTTCTG GCAGTTTGTG 
AAGGAAATGG ACAATGAGGT GCGGTTGCGG CTGCTGCAGT TTGTCACTGG TACCTGCAGG 
CTGCCGCTGG GAGGATTCGC AGAGCTCATG GGAAGCAACG GTCCACAGAA ATTCTGCATT 
GAGAAGGTTG GAAAAGAAAC ATGGCTCCCT CGAAGTCATA CATGTTTCAA TCGACTGGAC 
CTGCCGCCAT ATAAAAGCTA TGAACAACTG AAGGAGAAGC TGCTTTTTGC AATTGAAGAA 
ACGGAAGGAT TTGGCCAAGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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wwp1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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