pcnx1 gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_012969000.3	XP_012824454.2	pecanex-like protein 1 isoform X6
XM_004917251.3	XP_004917308.1	pecanex-like protein 1 isoform X1
XM_012969000.2	XP_012824454.1	pecanex-like protein 1 isoform X5
XM_004917253.4	XP_004917310.2	pecanex-like protein 1 isoform X3
XM_004917255.4	XP_004917312.2	pecanex-like protein 1 isoform X5
XM_004917252.3	XP_004917309.1	pecanex-like protein 1 isoform X2
XM_004917253.3	XP_004917310.1	pecanex-like protein 1 isoform X3
XM_004917251.4	XP_004917308.2	pecanex-like protein 1 isoform X1
XM_012969001.2	XP_012824455.1	pecanex-like protein 1 isoform X6
XM_004917252.4	XP_004917309.2	pecanex-like protein 1 isoform X2
XM_012969001.3	XP_012824455.2	pecanex-like protein 1 isoform X7
XM_004917255.3	XP_004917312.1	pecanex-like protein 1 isoform X4
XM_031890966.1	XP_031746826.1	pecanex-like protein 1 isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa64207	XM_004917251.4 Latest version!	Xenopus tropicalis pecanex 1 (pcnx1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OXa64208	XM_004917252.4 Latest version!	Xenopus tropicalis pecanex 1 (pcnx1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OXa64209	XM_004917253.4 Latest version!	Xenopus tropicalis pecanex 1 (pcnx1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa64210	XM_031890966.1 Latest version!	Xenopus tropicalis pecanex 1 (pcnx1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa64211	XM_004917255.4 Latest version!	Xenopus tropicalis pecanex 1 (pcnx1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa64212	XM_012969000.3 Latest version!	Xenopus tropicalis pecanex 1 (pcnx1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa64213	XM_012969001.3 Latest version!	Xenopus tropicalis pecanex 1 (pcnx1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa15003	XM_004917251.3 Latest version!	Xenopus tropicalis pecanex homolog (pcnx), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OXa15004	XM_004917252.3 Latest version!	Xenopus tropicalis pecanex homolog (pcnx), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OXa15005	XM_004917253.3 Latest version!	Xenopus tropicalis pecanex homolog (pcnx), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OXa15007	XM_004917255.3 Latest version!	Xenopus tropicalis pecanex homolog (pcnx), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OXa31182	XM_012969000.2 Latest version!	Xenopus tropicalis pecanex homolog (pcnx), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OXa31183	XM_012969001.2 Latest version!	Xenopus tropicalis pecanex homolog (pcnx), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

pcnx1 ( XM_004917251.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004917308.2 Xenopus tropicalis pecanex 1 (pcnx1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa64207
Clone ID Related Accession (Same CDS sequence)	XM_004917251.4
Accession Version	XM_004917251.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7026bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pecanex-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004917251.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCC AGACACTGCA AATCCTCCGG CAGGGAGTCT GGGCTTCCAT CACCGGAGGC 
TGGTACTACG ACCCCCACCA GAGTACCTTT GTCAATACCC TACACCTCTA CATCTGGCTC 
TTCCTCCTCT GCTTCCCCTT CACCCTCTAC ATGGCACTAC CCACATCTTT GGTGATTGTT 
GCTGTGTACT GCCCAGTCGT TGCAGCTGTT TTTATTGTTT TGAAGCTTGT TAACTACCGT 
CTGCACAGGG CATTGGATGA AGGAGAAATT GTGGAGAGGA GTGCTAGTGA GTTGACTGAG 
AGGGTGGCAA AGGCAGAACA CAGTGACAGC TCTGCAAGAA GAGAGGACAG CAATGGACCT 
GGTGATCCAG GTGGAGGGAT TGAAATGTCT GAATTTGTTC GAGAAGCAAC TCCTCCAGTT 
GGATGCAGTT CACGTAATTC CTACTCTGGG TTGGACCCAA GCAATCAGGT TGGTGTGTCT 
CGTTATGGAG GTGCATCTGG AAGCAAAGGG GATACAGATG CTGCAAAGAC ATCAGATGAT 
ATCAGTCTGA GTCTTGGCCA GAGTTACAGT TTGTGCAAAG AAGCAAGTGA AGACCAAGAT 
TTGACTGTGG ATCAGAAGCT TTTCCGTCTC GTCTCCAATG ACTCCTTTGT CTCCATGCAG 
CCATCTATAT CCTCGGGTGG AAAAGACTTA ACTAGAGACT TCAGTGACAA AGGATCAATC 
CCAACACACA GTCATCATCA CCATGTGGAT CTGTCTCTGT CTAATGCTTG TGACACAGAA 
TTGGCCTCAC TTGTTCCATT GCATTCACAT TCGTACAGGA AAGATCATCG ACCAAGAGGT 
GTCCCAAGGA CTTCAAGTTC TGCTGTTGCT TTCCCAGACA CTTCTCTCAC TGACTTTTCA 
CTCTATCAAC CAAGGCGCGG GCTGGATCCT GTTAGTGAAC TTGAAGCTTC CAAACCACAT 
TCTGGGTCCA AAGATTCACT AGTAGATAAT GCTTGTGCTT CAGGGATTTT TCAATTAGAC 
TGTCTAAAAA GCAAAGGAGC CCAGCAGCTT TCAAGAAGTG GTAGTGTTAA ACACATGAAG 
ACTGACAAAA GCATGGATAG TTTAAGAAGC CTCAGCACGA GAAGCAGTGG ATCCACAGAA 
AGTTATTGTA GTGGAACAGA TCGGGACACA AATAGCACTG TTAGCAGCTA CAAAAGTGAA 
CAGACCAGTT CAACTCATGT TGAAAGTATT TTATCTGAGC ATGAGGATGT TACTGCTGCA 
AAGAGAGTCC ATTGTAGTGA CCTGGAGGAA GAACATAGCT CTGCTACTGA TAAAAGGACT 
AGTAGTATAA GAACTGCTTT AGAAAGAAAC ACTGCTCAGG AAGCCAAGGA TGCCAACACA 
TCTGATGATG TACAAAGTCC AAGGGGTCTT AGCTCATCAG CATCAGAGGA AGCTAATAAG 
AATCCACACG CAAATGAGTT AACTGTACAA ACAGACTGGC TTCCAGGTAA ACTGTCAGTG 
AACATTGATG AACCTGGGGA GAAACCAGTC ATTTTAGTTG ACTTGAGATC TTTGGAAGAA 
ACGGGTATTA AGCAGAGAGA CAATGACGTA CGACCCAAAT CATCTAGTTT AATACATCGA 
ACATCCTCTG CTCATAAATC ATGCAGGAGA CGGACAGGTA AAAAAAGAGC CAGCAGTTTT 
GATGCAAGTA GACACAAAGA ATATGTTTCC TTTCAAGGTA TAGCTGATAC CAAACCTCAC 
AGTACTGGCT TTTGTCATGA TGAAGATTCT AGCGACCAAA GCGACTTGAG CAGGACCTCA 
AGCGTTCAGT CGGCCCACCG TTTCAGCAGC GATAGCTCTT CCAGCACTAC TTCCCATTCA 
TGCCAGTCTC CTGAAGCAAG ATACAGTGCC CTAAGGACAA AATATCGAAT AAAAGAAAGA 
GGCACAGATT CACATCACAC ACACCTAAAT TGTGAAGGAA CTAGTAAAAA GCGGTCCAAC 
CGCAGGACTT CTAGTGCAAG TGGTACAAAA AGCCGAGCTA GAGTATTAAG TCTGGACAGT 
GGCACTGTAG TTAGTTTGAG TGATGCCAAC AGACTGGCCG CTCCTGAAAA TATAAAAACC 
TTAACCACTT CAAAATCAGA TCTAGAAGCC AAAGAAGGCG AAGTGCTAGA TCAGATGTCT 
CTGTTGGGGC GAGCTTCACA GTTAGAATCT GTAACACGGT CAAGAAATAG TTTGCCCAGC 
CAGGTTACTT TCCAAGATGT TGGAGAGCGA GACGCATCTG TTGGAGCACA CGCCAGTGAG 
GAGACAGTAA CATTTCGCCG CGAACGCAGC ACATTTAGGC GCCAAGCGGT TCGGCGCAGA 
CACAATGCAG GGAGTAATCC CACCCCTCCT GCGTCACTCA TCGGATCACC TCTGAGCCTT 
CAAGATGGTC ATCAAGGCCA GCAGTCATCA ACCCAGGTCA AAGTACAGTC CCGCCCCCCT 
TCCCAGGCTG CAGTGCTCAG CGCTAGCGCC TCCTTGCTGG TGAGAAATGG GAGTGTCCAC 
TTAGAAGCGT CTCATGACAA TGCATCTGCC GTAGGCAGTA GCAGTTTGCA CGATGAACTT 
GGTATGCAGG CCTTATGTAA CCTTGGTGAC ATGGAAGTTT TAATGCAGCA CATTTTGGGT 
AAATTTACCC CATCTCTATA TGAGACTGGA GGTTGTGACA TGTCACTTGT AAACTTTGAG 
CCAGCTGCAA GAAGGACGTC TAATAATCTT TGTGACACAG ATTCCCATGT ATCAAGTTCT 
ACCTCAGTTC GATTCTACCC ACATGATCTA CTCTCTCTTC CCCAGATCCG ATTAAACAGG 
CTGCTAACCA TAGATACAGA TCTGTTAGAG CAGCAGGATA TTGATCTAAG CCCTGACCTG 
CAGGACCAGA TGCAACCACA GGAACAGGCT GCCCAAAAAG TAAAACACTA CTACCGGTTT 
TGGATTCTCC CAAAGCTGTG GGTCGGCATC AACTTTGACA GGCTTACACT CTTAGCCTTG 
TTCGATAGGA ACCGCGAGGT CCTAGAAAAT GTGCTGTCTG TCATTCTCGC CATTCTAGTT 
GCCTTTTTGG GCTCTGTTCT TCTCATAGAA GGCTTCTTTA AAGATATCTG GGTCTTCCAG 
TTTTGTCTTG TTATAGCCAG CTGTCAATAC TCTCTCCTTA AGAGTGTTCA ACCAGATTCT 
TCTTCCCCAA GACATGGCCA TAATCGTATC ATTGCCTATA GTAGGCCCGT TTACTTCTGT 
GCCTGTTGCG GCCTTATTTG GTTATTGGAT TATGGAAGCA AAAACATGTC TACCACCAAA 
TACAAACTAT ATGGAATGGC CTTCACCAAT CCTCTGCTTC TAGTGTCAGC CAGGGACTTA 
GTCATTGTCT TCACACTCTG TTTCCCAGTC GTGTTCTTTA TTGGTCTCCT GCCTCAGGTG 
AACACATTCG TCATGTACCT GTGTGAGCAA GTAGATATAC ATGTGTTTGG TGGAAATGCT 
ACCACCAGCT TGCTTGCAGC AATTTACAGC TTTATCCGTA GCATTGTGGC CATAGCTTTA 
CTCTACGGGC TGTGTTACGG TGCTCTTAAG GATCCATGGG ATGCACAACA CATTCCTGTA 
TTATTCTCTA TTTTCTGTGG TGTTCTTGTG GCAGTTTCAT ACCATCTCAG CCGACAGAGC 
AGCGATCCCT CTGTCCTCAT CTCACTGGTG CAATCAAAGA TTTTTCCAAA CAGCGAGGAT 
ACAAATCCTG AAGACCCACT ATCAGAAGTG AAAGACCCTC TTCCAGAAAA ACTGAGAAGC 
TCTGTTAATG AACGACTTCA GTCAGACGTA GTAGTCTGTG TCGTTATTGC AGTGTTGTAC 
TTTGCAATTC ATGTCAGCAC CGTATTTACT GCATTACAGC CGGTTCTGAG CTACGTACTC 
TATGCACTAG TTGGTTCTGT TGGACTCCTT ACTCACTACA TTCTACCACA AGTACGGAAA 
CAGCTTCCTT GGCACTGCTT CTCCCACCCG CTGCTGAAAA CTAAGGAATA TTACCAATTT 
GAAGTTCGAA ATGCTGCTCA TCTGATGTGG TTTGAGAAGG CTCACATCTG GCTGCTTTTT 
ATCGAGAAGA ACATTATTTA TCCTCTTATA GTTCTCAATG AGCTTAGCAT TGGTGCTAAG 
GATATCGTCA GCCCGAAAAA GCTGGATGCA GAAGTTGGTG CATTGTTGAT CACCATTGCC 
GGAATGAAGC TGCTGAGGTC GTCATTTAGC AGTCCCACCT GCCAGTATGC CACTGTCATT 
TTCACTGTCC TCTTCTTTAC TTTTGACTAC AAGGAGTATA CAGAAAAACT GCTGCTAGAT 
CTGTTCTTTA TGTCAATCCT CTTTAGCAAG CTCTGGGAAC TGTTTTTCAA ACTGCGCTTT 
GTCTACACCT ATATTGCCCC TTGGCAGATC ACGTGGGGAT CAGCATTCCA TGCTTTCGCA 
CAGCCATTTG CTGTACCTCA TTCTGCAATG CTGTTTGTAC AGGCAATTGT TTCTGCACTG 
TTCTCCACTC CCCTGAACCC TTTCTTAGGG AGTGCAATCT TTATTACATC ATATGTACGC 
CCTGTCAAGT TTTGGGAGAG AGACTACAAT ACAAAGAGAG TTGACCACTC AAACACAAGA 
TTAGCCTCTC AGCTTGACAG AAATCCAGGT TCAGATGACA ACAACTTGAA TTCCATATTT 
TATGAGCATC TCACACGCTC CCTTCAGCAT AGTCTGTGTG GTGATCTGTT GCTTGGCCGC 
TGGGGAAATT ACAATACTGG GGATTGCTTT ATTCTGGCCT CAGATTATCT AAACGCCTTG 
GTGCACCTTA TCGAAATAGG CAATGGTCTG GTCACCTTCC AGCTAAGAGG ACTAGAGTTC 
AGAGGTACCT ACTGCCAGCA GCGTGAAGTT GAAGCAATCA CTGAGGGTGT AGAGGAAGAT 
GAAGGCTTTT GCTGTTGCGA ACCTGGACAT ATTCCTCATA TGCTGTCTTT CAATGCTGCC 
TTTGGGCAGC GCTGGCTGGC ATGGGAAGTC TTGGTTACAA AGTATGTTCT GGAAGGTTAC 
AGCATCACAG ACAACAGTGC AGCCTCTATG CTTCAAGTGT TTGACCTTCG CAGGATCCTC 
ACTACCTATT ATGTTAAGGG CATTATATAC TATGTGACAG CATCTGCAAA ACTGGAGGAG 
TGGTTAGCAA ATGAGACAAT GCAGGAAGGT CTTCGCCAGT GTTCTGATCA TTGTTATGTT 
GATGTAGATC CAACTTTCAA CCCAAATATT GATGAAGACT ATGACCTTCG CTTAGCAGGC 
ATTTCACGGG AGAGCTTCTG TGCAAGCTAT CTTAACTGGA TTAAGTACTG TTCCTCTAGA 
AGGGAGAAGC CTCTTGATGC TGAAAAGGAT TCACCTTTGG TAACACTTTG TTATGGGCTC 
TGTATTTTGG GGAGAAGGGC ACTTGGTACA GCTTCTCATC ACATGTCCAG CAATTTGGAG 
TCCTTTCTAT ATGGTTTACA TGCCTTGTTT AAAGGAGATT TCCGCATATC ATCTCTCCGT 
GATGAGTGGA TTTTTGCTGA TATGGAGCTT TTAAGAAAAG TAGTAGTTCC TGGCATACGT 
ATGTCACTAA AACTTCATCA GGACCACTTT ACATCTCCAG ATGAGTATGA TGATCCTGCA 
GTTTTGTATG AAGCCATTAC ATCACATGAG CAGAACCTTG TTATTGCGCA TGAGGGAGAT 
CCAGCGTGGA GGAGTGCTGT TCTTTCTAAC TCACCTTCTC TTCTCGCGCT GCGTCATGTG 
ATGGATGATG GAACCAACGA ATACAAGATC ATAATGTTAA ATAAACGTTA TCTTAGTTTT 
AGAGTCATTA AGGTAAACAA GGAGTGTGTG CGGGGCTTAT GGGCTGGTCA GCAGCAAGAA 
TTGGTCTTTC TTCGTAATCG GAACCCTGAA AGAGGCAGCA TCCAAAATGC AAAGCAGGCT 
CTCAGGAATA TGATCAATTC ATCATGTGAC CAACCTATTG GATACCCAAT TTATGTTTCT 
CCACTGACAA CCTCTTATTC AGACAGCCAT GATCAGCTTA AACAGATATT TGGTGGGCCA 
ATTAGCTTGG CCAATATCCG GAACTTCATT GTATCTACAT GGCACAGATT AAGAAAAGGT 
TGCGGTGCAG GATGCAATAG TGGTGGAAAT ATTGAGGACT CTGACACTGG AGGCGGGACC 
TCCATTAATA GCAACAATCC ACCAAATGCA AATGAAACTC AGAGCAGTCT GTCACAAGTT 
GGTGTCCCAG GTGGAACAGG GCATGTGCCG AATGCACGGC TCCAGCCTCC GGGGATGCCT 
CATCCATCAA CACTAGGAAC CAGTCACAGT TCTCACTCCA TGCAGTCAAG CCTTGTCAGA 
CATTCCCCAG CTCGTGGATC AGTAGCAAGT CAATCATCTT ACTGCTACAA CAGCCGTCAC 
TCATCACTTA GAACATCCAC AACAGGATTT GTGCCTTGTA GGCGCTCCTC TACCAGCCAG 
ATATCTCTTC GCAATCTCCC CTCATCCATT CAGTCCCGTC TTTCCATGGT GAACCAAACA 
GACCCCACAG GACAGTCAGT TTTGGTTGGC CTTCCTTCCT CCAGCAGCTC AAGCCAGAGC 
ATCCCTGCAT GCAAAAGACA CACCTTAGTG GGCTTTTTAG GAACAGAAGG AGGCAATAAC 
CTCACTGAAA CGCAATCCTG TGGCAATTCT AGTCCAGCAA CCCATTCAAG AGCCAAAAAG 
GAAGAGGTTG TTTACAGAGT CCAGATTGTG GATCCAAGCC AAGTGCTTGA AGGCGTGAAT 
ATATCCAAAA GAAAGGAGCT GCAGTGGCCA GATGAGCTGG TGCGGCTAAA AGCCGGCAAG 
AACAGCTGGA AAGACTGGAG CCCTCAGGAA GGCATGGAAG GCCATGTAAT TCACCGCTGG 
GTGCCTTGCA GCAGAGACCC TTGTAATAGG TCCCACATTG ACAAAACAAT CCTGCTGATT 
AAGATTGAGG ACAAATATGT TGCTGTGATT GAAACTGGTG TGCTTGAACT CGGAGCTGAA 
GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004917308.2
CDS	277..7302
Translation	MGSQTLQILRQGVWASITGGWYYDPHQSTFVNTLHLYIWLFLLCFPFTLYMALPTSLVIVAVYCPVVAAVFIVLKLVNYRLHRALDEGEIVERSASELTERVAKAEHSDSSARREDSNGPGDPGGGIEMSEFVREATPPVGCSSRNSYSGLDPSNQVGVSRYGGASGSKGDTDAAKTSDDISLSLGQSYSLCKEASEDQDLTVDQKLFRLVSNDSFVSMQPSISSGGKDLTRDFSDKGSIPTHSHHHHVDLSLSNACDTELASLVPLHSHSYRKDHRPRGVPRTSSSAVAFPDTSLTDFSLYQPRRGLDPVSELEASKPHSGSKDSLVDNACASGIFQLDCLKSKGAQQLSRSGSVKHMKTDKSMDSLRSLSTRSSGSTESYCSGTDRDTNSTVSSYKSEQTSSTHVESILSEHEDVTAAKRVHCSDLEEEHSSATDKRTSSIRTALERNTAQEAKDANTSDDVQSPRGLSSSASEEANKNPHANELTVQTDWLPGKLSVNIDEPGEKPVILVDLRSLEETGIKQRDNDVRPKSSSLIHRTSSAHKSCRRRTGKKRASSFDASRHKEYVSFQGIADTKPHSTGFCHDEDSSDQSDLSRTSSVQSAHRFSSDSSSSTTSHSCQSPEARYSALRTKYRIKERGTDSHHTHLNCEGTSKKRSNRRTSSASGTKSRARVLSLDSGTVVSLSDANRLAAPENIKTLTTSKSDLEAKEGEVLDQMSLLGRASQLESVTRSRNSLPSQVTFQDVGERDASVGAHASEETVTFRRERSTFRRQAVRRRHNAGSNPTPPASLIGSPLSLQDGHQGQQSSTQVKVQSRPPSQAAVLSASASLLVRNGSVHLEASHDNASAVGSSSLHDELGMQALCNLGDMEVLMQHILGKFTPSLYETGGCDMSLVNFEPAARRTSNNLCDTDSHVSSSTSVRFYPHDLLSLPQIRLNRLLTIDTDLLEQQDIDLSPDLQDQMQPQEQAAQKVKHYYRFWILPKLWVGINFDRLTLLALFDRNREVLENVLSVILAILVAFLGSVLLIEGFFKDIWVFQFCLVIASCQYSLLKSVQPDSSSPRHGHNRIIAYSRPVYFCACCGLIWLLDYGSKNMSTTKYKLYGMAFTNPLLLVSARDLVIVFTLCFPVVFFIGLLPQVNTFVMYLCEQVDIHVFGGNATTSLLAAIYSFIRSIVAIALLYGLCYGALKDPWDAQHIPVLFSIFCGVLVAVSYHLSRQSSDPSVLISLVQSKIFPNSEDTNPEDPLSEVKDPLPEKLRSSVNERLQSDVVVCVVIAVLYFAIHVSTVFTALQPVLSYVLYALVGSVGLLTHYILPQVRKQLPWHCFSHPLLKTKEYYQFEVRNAAHLMWFEKAHIWLLFIEKNIIYPLIVLNELSIGAKDIVSPKKLDAEVGALLITIAGMKLLRSSFSSPTCQYATVIFTVLFFTFDYKEYTEKLLLDLFFMSILFSKLWELFFKLRFVYTYIAPWQITWGSAFHAFAQPFAVPHSAMLFVQAIVSALFSTPLNPFLGSAIFITSYVRPVKFWERDYNTKRVDHSNTRLASQLDRNPGSDDNNLNSIFYEHLTRSLQHSLCGDLLLGRWGNYNTGDCFILASDYLNALVHLIEIGNGLVTFQLRGLEFRGTYCQQREVEAITEGVEEDEGFCCCEPGHIPHMLSFNAAFGQRWLAWEVLVTKYVLEGYSITDNSAASMLQVFDLRRILTTYYVKGIIYYVTASAKLEEWLANETMQEGLRQCSDHCYVDVDPTFNPNIDEDYDLRLAGISRESFCASYLNWIKYCSSRREKPLDAEKDSPLVTLCYGLCILGRRALGTASHHMSSNLESFLYGLHALFKGDFRISSLRDEWIFADMELLRKVVVPGIRMSLKLHQDHFTSPDEYDDPAVLYEAITSHEQNLVIAHEGDPAWRSAVLSNSPSLLALRHVMDDGTNEYKIIMLNKRYLSFRVIKVNKECVRGLWAGQQQELVFLRNRNPERGSIQNAKQALRNMINSSCDQPIGYPIYVSPLTTSYSDSHDQLKQIFGGPISLANIRNFIVSTWHRLRKGCGAGCNSGGNIEDSDTGGGTSINSNNPPNANETQSSLSQVGVPGGTGHVPNARLQPPGMPHPSTLGTSHSSHSMQSSLVRHSPARGSVASQSSYCYNSRHSSLRTSTTGFVPCRRSSTSQISLRNLPSSIQSRLSMVNQTDPTGQSVLVGLPSSSSSSQSIPACKRHTLVGFLGTEGGNNLTETQSCGNSSPATHSRAKKEEVVYRVQIVDPSQVLEGVNISKRKELQWPDELVRLKAGKNSWKDWSPQEGMEGHVIHRWVPCSRDPCNRSHIDKTILLIKIEDKYVAVIETGVLELGAEV

Target ORF information:

RefSeq Version	XM_004917251.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pecanex 1 (pcnx1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004917251.4

ORF Insert Sequence:

ATGGGTTCCC AGACACTGCA AATCCTCCGG CAGGGAGTCT GGGCTTCCAT CACCGGAGGC 
TGGTACTACG ACCCCCACCA GAGTACCTTT GTCAATACCC TACACCTCTA CATCTGGCTC 
TTCCTCCTCT GCTTCCCCTT CACCCTCTAC ATGGCACTAC CCACATCTTT GGTGATTGTT 
GCTGTGTACT GCCCAGTCGT TGCAGCTGTT TTTATTGTTT TGAAGCTTGT TAACTACCGT 
CTGCACAGGG CATTGGATGA AGGAGAAATT GTGGAGAGGA GTGCTAGTGA GTTGACTGAG 
AGGGTGGCAA AGGCAGAACA CAGTGACAGC TCTGCAAGAA GAGAGGACAG CAATGGACCT 
GGTGATCCAG GTGGAGGGAT TGAAATGTCT GAATTTGTTC GAGAAGCAAC TCCTCCAGTT 
GGATGCAGTT CACGTAATTC CTACTCTGGG TTGGACCCAA GCAATCAGGT TGGTGTGTCT 
CGTTATGGAG GTGCATCTGG AAGCAAAGGG GATACAGATG CTGCAAAGAC ATCAGATGAT 
ATCAGTCTGA GTCTTGGCCA GAGTTACAGT TTGTGCAAAG AAGCAAGTGA AGACCAAGAT 
TTGACTGTGG ATCAGAAGCT TTTCCGTCTC GTCTCCAATG ACTCCTTTGT CTCCATGCAG 
CCATCTATAT CCTCGGGTGG AAAAGACTTA ACTAGAGACT TCAGTGACAA AGGATCAATC 
CCAACACACA GTCATCATCA CCATGTGGAT CTGTCTCTGT CTAATGCTTG TGACACAGAA 
TTGGCCTCAC TTGTTCCATT GCATTCACAT TCGTACAGGA AAGATCATCG ACCAAGAGGT 
GTCCCAAGGA CTTCAAGTTC TGCTGTTGCT TTCCCAGACA CTTCTCTCAC TGACTTTTCA 
CTCTATCAAC CAAGGCGCGG GCTGGATCCT GTTAGTGAAC TTGAAGCTTC CAAACCACAT 
TCTGGGTCCA AAGATTCACT AGTAGATAAT GCTTGTGCTT CAGGGATTTT TCAATTAGAC 
TGTCTAAAAA GCAAAGGAGC CCAGCAGCTT TCAAGAAGTG GTAGTGTTAA ACACATGAAG 
ACTGACAAAA GCATGGATAG TTTAAGAAGC CTCAGCACGA GAAGCAGTGG ATCCACAGAA 
AGTTATTGTA GTGGAACAGA TCGGGACACA AATAGCACTG TTAGCAGCTA CAAAAGTGAA 
CAGACCAGTT CAACTCATGT TGAAAGTATT TTATCTGAGC ATGAGGATGT TACTGCTGCA 
AAGAGAGTCC ATTGTAGTGA CCTGGAGGAA GAACATAGCT CTGCTACTGA TAAAAGGACT 
AGTAGTATAA GAACTGCTTT AGAAAGAAAC ACTGCTCAGG AAGCCAAGGA TGCCAACACA 
TCTGATGATG TACAAAGTCC AAGGGGTCTT AGCTCATCAG CATCAGAGGA AGCTAATAAG 
AATCCACACG CAAATGAGTT AACTGTACAA ACAGACTGGC TTCCAGGTAA ACTGTCAGTG 
AACATTGATG AACCTGGGGA GAAACCAGTC ATTTTAGTTG ACTTGAGATC TTTGGAAGAA 
ACGGGTATTA AGCAGAGAGA CAATGACGTA CGACCCAAAT CATCTAGTTT AATACATCGA 
ACATCCTCTG CTCATAAATC ATGCAGGAGA CGGACAGGTA AAAAAAGAGC CAGCAGTTTT 
GATGCAAGTA GACACAAAGA ATATGTTTCC TTTCAAGGTA TAGCTGATAC CAAACCTCAC 
AGTACTGGCT TTTGTCATGA TGAAGATTCT AGCGACCAAA GCGACTTGAG CAGGACCTCA 
AGCGTTCAGT CGGCCCACCG TTTCAGCAGC GATAGCTCTT CCAGCACTAC TTCCCATTCA 
TGCCAGTCTC CTGAAGCAAG ATACAGTGCC CTAAGGACAA AATATCGAAT AAAAGAAAGA 
GGCACAGATT CACATCACAC ACACCTAAAT TGTGAAGGAA CTAGTAAAAA GCGGTCCAAC 
CGCAGGACTT CTAGTGCAAG TGGTACAAAA AGCCGAGCTA GAGTATTAAG TCTGGACAGT 
GGCACTGTAG TTAGTTTGAG TGATGCCAAC AGACTGGCCG CTCCTGAAAA TATAAAAACC 
TTAACCACTT CAAAATCAGA TCTAGAAGCC AAAGAAGGCG AAGTGCTAGA TCAGATGTCT 
CTGTTGGGGC GAGCTTCACA GTTAGAATCT GTAACACGGT CAAGAAATAG TTTGCCCAGC 
CAGGTTACTT TCCAAGATGT TGGAGAGCGA GACGCATCTG TTGGAGCACA CGCCAGTGAG 
GAGACAGTAA CATTTCGCCG CGAACGCAGC ACATTTAGGC GCCAAGCGGT TCGGCGCAGA 
CACAATGCAG GGAGTAATCC CACCCCTCCT GCGTCACTCA TCGGATCACC TCTGAGCCTT 
CAAGATGGTC ATCAAGGCCA GCAGTCATCA ACCCAGGTCA AAGTACAGTC CCGCCCCCCT 
TCCCAGGCTG CAGTGCTCAG CGCTAGCGCC TCCTTGCTGG TGAGAAATGG GAGTGTCCAC 
TTAGAAGCGT CTCATGACAA TGCATCTGCC GTAGGCAGTA GCAGTTTGCA CGATGAACTT 
GGTATGCAGG CCTTATGTAA CCTTGGTGAC ATGGAAGTTT TAATGCAGCA CATTTTGGGT 
AAATTTACCC CATCTCTATA TGAGACTGGA GGTTGTGACA TGTCACTTGT AAACTTTGAG 
CCAGCTGCAA GAAGGACGTC TAATAATCTT TGTGACACAG ATTCCCATGT ATCAAGTTCT 
ACCTCAGTTC GATTCTACCC ACATGATCTA CTCTCTCTTC CCCAGATCCG ATTAAACAGG 
CTGCTAACCA TAGATACAGA TCTGTTAGAG CAGCAGGATA TTGATCTAAG CCCTGACCTG 
CAGGACCAGA TGCAACCACA GGAACAGGCT GCCCAAAAAG TAAAACACTA CTACCGGTTT 
TGGATTCTCC CAAAGCTGTG GGTCGGCATC AACTTTGACA GGCTTACACT CTTAGCCTTG 
TTCGATAGGA ACCGCGAGGT CCTAGAAAAT GTGCTGTCTG TCATTCTCGC CATTCTAGTT 
GCCTTTTTGG GCTCTGTTCT TCTCATAGAA GGCTTCTTTA AAGATATCTG GGTCTTCCAG 
TTTTGTCTTG TTATAGCCAG CTGTCAATAC TCTCTCCTTA AGAGTGTTCA ACCAGATTCT 
TCTTCCCCAA GACATGGCCA TAATCGTATC ATTGCCTATA GTAGGCCCGT TTACTTCTGT 
GCCTGTTGCG GCCTTATTTG GTTATTGGAT TATGGAAGCA AAAACATGTC TACCACCAAA 
TACAAACTAT ATGGAATGGC CTTCACCAAT CCTCTGCTTC TAGTGTCAGC CAGGGACTTA 
GTCATTGTCT TCACACTCTG TTTCCCAGTC GTGTTCTTTA TTGGTCTCCT GCCTCAGGTG 
AACACATTCG TCATGTACCT GTGTGAGCAA GTAGATATAC ATGTGTTTGG TGGAAATGCT 
ACCACCAGCT TGCTTGCAGC AATTTACAGC TTTATCCGTA GCATTGTGGC CATAGCTTTA 
CTCTACGGGC TGTGTTACGG TGCTCTTAAG GATCCATGGG ATGCACAACA CATTCCTGTA 
TTATTCTCTA TTTTCTGTGG TGTTCTTGTG GCAGTTTCAT ACCATCTCAG CCGACAGAGC 
AGCGATCCCT CTGTCCTCAT CTCACTGGTG CAATCAAAGA TTTTTCCAAA CAGCGAGGAT 
ACAAATCCTG AAGACCCACT ATCAGAAGTG AAAGACCCTC TTCCAGAAAA ACTGAGAAGC 
TCTGTTAATG AACGACTTCA GTCAGACGTA GTAGTCTGTG TCGTTATTGC AGTGTTGTAC 
TTTGCAATTC ATGTCAGCAC CGTATTTACT GCATTACAGC CGGTTCTGAG CTACGTACTC 
TATGCACTAG TTGGTTCTGT TGGACTCCTT ACTCACTACA TTCTACCACA AGTACGGAAA 
CAGCTTCCTT GGCACTGCTT CTCCCACCCG CTGCTGAAAA CTAAGGAATA TTACCAATTT 
GAAGTTCGAA ATGCTGCTCA TCTGATGTGG TTTGAGAAGG CTCACATCTG GCTGCTTTTT 
ATCGAGAAGA ACATTATTTA TCCTCTTATA GTTCTCAATG AGCTTAGCAT TGGTGCTAAG 
GATATCGTCA GCCCGAAAAA GCTGGATGCA GAAGTTGGTG CATTGTTGAT CACCATTGCC 
GGAATGAAGC TGCTGAGGTC GTCATTTAGC AGTCCCACCT GCCAGTATGC CACTGTCATT 
TTCACTGTCC TCTTCTTTAC TTTTGACTAC AAGGAGTATA CAGAAAAACT GCTGCTAGAT 
CTGTTCTTTA TGTCAATCCT CTTTAGCAAG CTCTGGGAAC TGTTTTTCAA ACTGCGCTTT 
GTCTACACCT ATATTGCCCC TTGGCAGATC ACGTGGGGAT CAGCATTCCA TGCTTTCGCA 
CAGCCATTTG CTGTACCTCA TTCTGCAATG CTGTTTGTAC AGGCAATTGT TTCTGCACTG 
TTCTCCACTC CCCTGAACCC TTTCTTAGGG AGTGCAATCT TTATTACATC ATATGTACGC 
CCTGTCAAGT TTTGGGAGAG AGACTACAAT ACAAAGAGAG TTGACCACTC AAACACAAGA 
TTAGCCTCTC AGCTTGACAG AAATCCAGGT TCAGATGACA ACAACTTGAA TTCCATATTT 
TATGAGCATC TCACACGCTC CCTTCAGCAT AGTCTGTGTG GTGATCTGTT GCTTGGCCGC 
TGGGGAAATT ACAATACTGG GGATTGCTTT ATTCTGGCCT CAGATTATCT AAACGCCTTG 
GTGCACCTTA TCGAAATAGG CAATGGTCTG GTCACCTTCC AGCTAAGAGG ACTAGAGTTC 
AGAGGTACCT ACTGCCAGCA GCGTGAAGTT GAAGCAATCA CTGAGGGTGT AGAGGAAGAT 
GAAGGCTTTT GCTGTTGCGA ACCTGGACAT ATTCCTCATA TGCTGTCTTT CAATGCTGCC 
TTTGGGCAGC GCTGGCTGGC ATGGGAAGTC TTGGTTACAA AGTATGTTCT GGAAGGTTAC 
AGCATCACAG ACAACAGTGC AGCCTCTATG CTTCAAGTGT TTGACCTTCG CAGGATCCTC 
ACTACCTATT ATGTTAAGGG CATTATATAC TATGTGACAG CATCTGCAAA ACTGGAGGAG 
TGGTTAGCAA ATGAGACAAT GCAGGAAGGT CTTCGCCAGT GTTCTGATCA TTGTTATGTT 
GATGTAGATC CAACTTTCAA CCCAAATATT GATGAAGACT ATGACCTTCG CTTAGCAGGC 
ATTTCACGGG AGAGCTTCTG TGCAAGCTAT CTTAACTGGA TTAAGTACTG TTCCTCTAGA 
AGGGAGAAGC CTCTTGATGC TGAAAAGGAT TCACCTTTGG TAACACTTTG TTATGGGCTC 
TGTATTTTGG GGAGAAGGGC ACTTGGTACA GCTTCTCATC ACATGTCCAG CAATTTGGAG 
TCCTTTCTAT ATGGTTTACA TGCCTTGTTT AAAGGAGATT TCCGCATATC ATCTCTCCGT 
GATGAGTGGA TTTTTGCTGA TATGGAGCTT TTAAGAAAAG TAGTAGTTCC TGGCATACGT 
ATGTCACTAA AACTTCATCA GGACCACTTT ACATCTCCAG ATGAGTATGA TGATCCTGCA 
GTTTTGTATG AAGCCATTAC ATCACATGAG CAGAACCTTG TTATTGCGCA TGAGGGAGAT 
CCAGCGTGGA GGAGTGCTGT TCTTTCTAAC TCACCTTCTC TTCTCGCGCT GCGTCATGTG 
ATGGATGATG GAACCAACGA ATACAAGATC ATAATGTTAA ATAAACGTTA TCTTAGTTTT 
AGAGTCATTA AGGTAAACAA GGAGTGTGTG CGGGGCTTAT GGGCTGGTCA GCAGCAAGAA 
TTGGTCTTTC TTCGTAATCG GAACCCTGAA AGAGGCAGCA TCCAAAATGC AAAGCAGGCT 
CTCAGGAATA TGATCAATTC ATCATGTGAC CAACCTATTG GATACCCAAT TTATGTTTCT 
CCACTGACAA CCTCTTATTC AGACAGCCAT GATCAGCTTA AACAGATATT TGGTGGGCCA 
ATTAGCTTGG CCAATATCCG GAACTTCATT GTATCTACAT GGCACAGATT AAGAAAAGGT 
TGCGGTGCAG GATGCAATAG TGGTGGAAAT ATTGAGGACT CTGACACTGG AGGCGGGACC 
TCCATTAATA GCAACAATCC ACCAAATGCA AATGAAACTC AGAGCAGTCT GTCACAAGTT 
GGTGTCCCAG GTGGAACAGG GCATGTGCCG AATGCACGGC TCCAGCCTCC GGGGATGCCT 
CATCCATCAA CACTAGGAAC CAGTCACAGT TCTCACTCCA TGCAGTCAAG CCTTGTCAGA 
CATTCCCCAG CTCGTGGATC AGTAGCAAGT CAATCATCTT ACTGCTACAA CAGCCGTCAC 
TCATCACTTA GAACATCCAC AACAGGATTT GTGCCTTGTA GGCGCTCCTC TACCAGCCAG 
ATATCTCTTC GCAATCTCCC CTCATCCATT CAGTCCCGTC TTTCCATGGT GAACCAAACA 
GACCCCACAG GACAGTCAGT TTTGGTTGGC CTTCCTTCCT CCAGCAGCTC AAGCCAGAGC 
ATCCCTGCAT GCAAAAGACA CACCTTAGTG GGCTTTTTAG GAACAGAAGG AGGCAATAAC 
CTCACTGAAA CGCAATCCTG TGGCAATTCT AGTCCAGCAA CCCATTCAAG AGCCAAAAAG 
GAAGAGGTTG TTTACAGAGT CCAGATTGTG GATCCAAGCC AAGTGCTTGA AGGCGTGAAT 
ATATCCAAAA GAAAGGAGCT GCAGTGGCCA GATGAGCTGG TGCGGCTAAA AGCCGGCAAG 
AACAGCTGGA AAGACTGGAG CCCTCAGGAA GGCATGGAAG GCCATGTAAT TCACCGCTGG 
GTGCCTTGCA GCAGAGACCC TTGTAATAGG TCCCACATTG ACAAAACAAT CCTGCTGATT 
AAGATTGAGG ACAAATATGT TGCTGTGATT GAAACTGGTG TGCTTGAACT CGGAGCTGAA 
GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pcnx1 ( XM_004917252.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004917309.2 Xenopus tropicalis pecanex 1 (pcnx1), transcript variant X2, mRNA.

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CloneID	OXa64208
Clone ID Related Accession (Same CDS sequence)	XM_004917252.4
Accession Version	XM_004917252.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7011bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pecanex-like protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004917252.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCC AGACACTGCA AATCCTCCGG CAGGGAGTCT GGGCTTCCAT CACCGGAGGC 
TGGTACTACG ACCCCCACCA GAGTACCTTT GTCAATACCC TACACCTCTA CATCTGGCTC 
TTCCTCCTCT GCTTCCCCTT CACCCTCTAC ATGGCACTAC CCACATCTTT GGTGATTGTT 
GCTGTGTACT GCCCAGTCGT TGCAGCTGTT TTTATTGTTT TGAAGCTTGT TAACTACCGT 
CTGCACAGGG CATTGGATGA AGGAGAAATT GTGGAGAGGA GTGCTAGTGA GTTGACTGAG 
AGGGTGGCAA AGGCAGAACA CAGTGACAGC TCTGCAAGAA GAGAGGACAG CAATGGACCT 
GGTGATCCAG GTGGAGGGAT TGAAATGTCT GAATTTGTTC GAGAAGCAAC TCCTCCAGTT 
GGATGCAGTT CACGTAATTC CTACTCTGGG TTGGACCCAA GCAATCAGGT TGGTGTGTCT 
CGTTATGGAG GTGCATCTGG AAGCAAAGGG GATACAGATG CTGCAAAGAC ATCAGATGAT 
ATCAGTCTGA GTCTTGGCCA GAGTTACAGT TTGTGCAAAG AAGCAAGTGA AGACCAAGAT 
TTGACTGTGG ATCAGAAGCT TTTCCGTCTC GTCTCCAATG ACTCCTTTGT CTCCATGCAG 
CCATCTATAT CCTCGGGTGG AAAAGACTTA ACTAGAGACT TCAGTGACAA AGGATCAATC 
CCAACACACA GTCATCATCA CCATGTGGAT CTGTCTCTGT CTAATGCTTG TGACACAGAA 
TTGGCCTCAC TTGTTCCATT GCATTCACAT TCGTACAGGA AAGATCATCG ACCAAGAGGT 
GTCCCAAGGA CTTCAAGTTC TGCTGTTGCT TTCCCAGACA CTTCTCTCAC TGACTTTTCA 
CTCTATCAAC CAAGGCGCGG GCTGGATCCT GTTAGTGAAC TTGAAGCTTC CAAACCACAT 
TCTGGGTCCA AAGATTCACT AGTAGATAAT GCTTGTGCTT CAGGGATTTT TCAATTAGAC 
TGTCTAAAAA GCAAAGGAGC CCAGCAGCTT TCAAGAAGTG GTAGTGTTAA ACACATGAAG 
ACTGACAAAA GCATGGATAG TTTAAGAAGC CTCAGCACGA GAAGCAGTGG ATCCACAGAA 
AGTTATTGTA GTGGAACAGA TCGGGACACA AATAGCACTG TTAGCAGCTA CAAAAGTGAA 
CAGACCAGTT CAACTCATGT TGAAAGTATT TTATCTGAGC ATGAGGATGT TACTGCTGCA 
AAGAGAGTCC ATTGTAGTGA CCTGGAGGAA GAACATAGCT CTGCTACTGA TAAAAGGACT 
AGTAGTATAA GAACTGCTTT AGAAAGAAAC ACTGCTCAGG AAGCCAAGGA TGCCAACACA 
TCTGATGATG TACAAAGTCC AAGGGGTCTT AGCTCATCAG CATCAGAGGA AGCTAATAAG 
AATCCACACG CAAATGAGTT AACTGTACAA ACAGACTGGC TTCCAGGTAA ACTGTCAGTG 
AACATTGATG AACCTGGGGA GAAACCAGTC ATTTTAGTTG ACTTGAGATC TTTGGAAGAA 
ACGGGTATTA AGCAGAGAGA CAATGACGTA CGACCCAAAT CATCTAGTTT AATACATCGA 
ACATCCTCTG CTCATAAATC ATGCAGGAGA CGGACAGGTA AAAAAAGAGC CAGCAGTTTT 
GATGCAAGTA GACACAAAGA ATATGTTTCC TTTCAAGGTA TAGCTGATAC CAAACCTCAC 
AGTACTGGCT TTTGTCATGA TGAAGATTCT AGCGACCAAA GCGACTTGAG CAGGACCTCA 
AGCGTTCAGT CGGCCCACCG TTTCAGCAGC GATAGCTCTT CCAGCACTAC TTCCCATTCA 
TGCCAGTCTC CTGAAGCAAG ATACAGTGCC CTAAGGACAA AATATCGAAT AAAAGAAAGA 
GGCACAGATT CACATCACAC ACACCTAAAT TGTGAAGGAA CTAGTAAAAA GCGGTCCAAC 
CGCAGGACTT CTAGTGCAAG TGGTACAAAA AGCCGAGCTA GAGTATTAAG TCTGGACAGT 
GGCACTGTAG TTAGTTTGAG TGATGCCAAC AGACTGGCCG CTCCTGAAAA TATAAAAACC 
TTAACCACTT CAAAATCAGA TCTAGAAGCC AAAGAAGGCG AAGTGCTAGA TCAGATGTCT 
CTGTTGGGGC GAGCTTCACA GTTAGAATCT GTAACACGGT CAAGAAATAG TTTGCCCAGC 
CAGGTTACTT TCCAAGATGT TGGAGAGCGA GACGCATCTG TTGGAGCACA CGCCAGTGAG 
GAGACAGTAA CATTTCGCCG CGAACGCAGC ACATTTAGGC GCCAAGCGGT TCGGCGCAGA 
CACAATGCAG GGAGTAATCC CACCCCTCCT GCGTCACTCA TCGGATCACC TCTGAGCCTT 
CAAGATGGTC ATCAAGGCCA GCAGTCATCA ACCCAGGTCA AAGTACAGTC CCGCCCCCCT 
TCCCAGGCTG CAGTGCTCAG CGCTAGCGCC TCCTTGCTGG TGAGAAATGG GAGTGTCCAC 
TTAGAAGCGT CTCATGACAA TGCATCTGCC GTAGGCAGTA GCAGTTTGCA CGATGAACTT 
GGTATGCAGG CCTTATGTAA CCTTGGTGAC ATGGAAGTTT TAATGCAGCA CATTTTGGGT 
AAATTTACCC CATCTCTATA TGAGACTGGA GGTTGTGACA TGTCACTTGT AAACTTTGAG 
CCAGCTGCAA GAAGGACGTC TAATAATCTT TGTGACACAG ATTCCCATGT ATCAAGTTCT 
ACCTCAGTTC GATTCTACCC ACATGATCTA ATCCGATTAA ACAGGCTGCT AACCATAGAT 
ACAGATCTGT TAGAGCAGCA GGATATTGAT CTAAGCCCTG ACCTGCAGGA CCAGATGCAA 
CCACAGGAAC AGGCTGCCCA AAAAGTAAAA CACTACTACC GGTTTTGGAT TCTCCCAAAG 
CTGTGGGTCG GCATCAACTT TGACAGGCTT ACACTCTTAG CCTTGTTCGA TAGGAACCGC 
GAGGTCCTAG AAAATGTGCT GTCTGTCATT CTCGCCATTC TAGTTGCCTT TTTGGGCTCT 
GTTCTTCTCA TAGAAGGCTT CTTTAAAGAT ATCTGGGTCT TCCAGTTTTG TCTTGTTATA 
GCCAGCTGTC AATACTCTCT CCTTAAGAGT GTTCAACCAG ATTCTTCTTC CCCAAGACAT 
GGCCATAATC GTATCATTGC CTATAGTAGG CCCGTTTACT TCTGTGCCTG TTGCGGCCTT 
ATTTGGTTAT TGGATTATGG AAGCAAAAAC ATGTCTACCA CCAAATACAA ACTATATGGA 
ATGGCCTTCA CCAATCCTCT GCTTCTAGTG TCAGCCAGGG ACTTAGTCAT TGTCTTCACA 
CTCTGTTTCC CAGTCGTGTT CTTTATTGGT CTCCTGCCTC AGGTGAACAC ATTCGTCATG 
TACCTGTGTG AGCAAGTAGA TATACATGTG TTTGGTGGAA ATGCTACCAC CAGCTTGCTT 
GCAGCAATTT ACAGCTTTAT CCGTAGCATT GTGGCCATAG CTTTACTCTA CGGGCTGTGT 
TACGGTGCTC TTAAGGATCC ATGGGATGCA CAACACATTC CTGTATTATT CTCTATTTTC 
TGTGGTGTTC TTGTGGCAGT TTCATACCAT CTCAGCCGAC AGAGCAGCGA TCCCTCTGTC 
CTCATCTCAC TGGTGCAATC AAAGATTTTT CCAAACAGCG AGGATACAAA TCCTGAAGAC 
CCACTATCAG AAGTGAAAGA CCCTCTTCCA GAAAAACTGA GAAGCTCTGT TAATGAACGA 
CTTCAGTCAG ACGTAGTAGT CTGTGTCGTT ATTGCAGTGT TGTACTTTGC AATTCATGTC 
AGCACCGTAT TTACTGCATT ACAGCCGGTT CTGAGCTACG TACTCTATGC ACTAGTTGGT 
TCTGTTGGAC TCCTTACTCA CTACATTCTA CCACAAGTAC GGAAACAGCT TCCTTGGCAC 
TGCTTCTCCC ACCCGCTGCT GAAAACTAAG GAATATTACC AATTTGAAGT TCGAAATGCT 
GCTCATCTGA TGTGGTTTGA GAAGGCTCAC ATCTGGCTGC TTTTTATCGA GAAGAACATT 
ATTTATCCTC TTATAGTTCT CAATGAGCTT AGCATTGGTG CTAAGGATAT CGTCAGCCCG 
AAAAAGCTGG ATGCAGAAGT TGGTGCATTG TTGATCACCA TTGCCGGAAT GAAGCTGCTG 
AGGTCGTCAT TTAGCAGTCC CACCTGCCAG TATGCCACTG TCATTTTCAC TGTCCTCTTC 
TTTACTTTTG ACTACAAGGA GTATACAGAA AAACTGCTGC TAGATCTGTT CTTTATGTCA 
ATCCTCTTTA GCAAGCTCTG GGAACTGTTT TTCAAACTGC GCTTTGTCTA CACCTATATT 
GCCCCTTGGC AGATCACGTG GGGATCAGCA TTCCATGCTT TCGCACAGCC ATTTGCTGTA 
CCTCATTCTG CAATGCTGTT TGTACAGGCA ATTGTTTCTG CACTGTTCTC CACTCCCCTG 
AACCCTTTCT TAGGGAGTGC AATCTTTATT ACATCATATG TACGCCCTGT CAAGTTTTGG 
GAGAGAGACT ACAATACAAA GAGAGTTGAC CACTCAAACA CAAGATTAGC CTCTCAGCTT 
GACAGAAATC CAGGTTCAGA TGACAACAAC TTGAATTCCA TATTTTATGA GCATCTCACA 
CGCTCCCTTC AGCATAGTCT GTGTGGTGAT CTGTTGCTTG GCCGCTGGGG AAATTACAAT 
ACTGGGGATT GCTTTATTCT GGCCTCAGAT TATCTAAACG CCTTGGTGCA CCTTATCGAA 
ATAGGCAATG GTCTGGTCAC CTTCCAGCTA AGAGGACTAG AGTTCAGAGG TACCTACTGC 
CAGCAGCGTG AAGTTGAAGC AATCACTGAG GGTGTAGAGG AAGATGAAGG CTTTTGCTGT 
TGCGAACCTG GACATATTCC TCATATGCTG TCTTTCAATG CTGCCTTTGG GCAGCGCTGG 
CTGGCATGGG AAGTCTTGGT TACAAAGTAT GTTCTGGAAG GTTACAGCAT CACAGACAAC 
AGTGCAGCCT CTATGCTTCA AGTGTTTGAC CTTCGCAGGA TCCTCACTAC CTATTATGTT 
AAGGGCATTA TATACTATGT GACAGCATCT GCAAAACTGG AGGAGTGGTT AGCAAATGAG 
ACAATGCAGG AAGGTCTTCG CCAGTGTTCT GATCATTGTT ATGTTGATGT AGATCCAACT 
TTCAACCCAA ATATTGATGA AGACTATGAC CTTCGCTTAG CAGGCATTTC ACGGGAGAGC 
TTCTGTGCAA GCTATCTTAA CTGGATTAAG TACTGTTCCT CTAGAAGGGA GAAGCCTCTT 
GATGCTGAAA AGGATTCACC TTTGGTAACA CTTTGTTATG GGCTCTGTAT TTTGGGGAGA 
AGGGCACTTG GTACAGCTTC TCATCACATG TCCAGCAATT TGGAGTCCTT TCTATATGGT 
TTACATGCCT TGTTTAAAGG AGATTTCCGC ATATCATCTC TCCGTGATGA GTGGATTTTT 
GCTGATATGG AGCTTTTAAG AAAAGTAGTA GTTCCTGGCA TACGTATGTC ACTAAAACTT 
CATCAGGACC ACTTTACATC TCCAGATGAG TATGATGATC CTGCAGTTTT GTATGAAGCC 
ATTACATCAC ATGAGCAGAA CCTTGTTATT GCGCATGAGG GAGATCCAGC GTGGAGGAGT 
GCTGTTCTTT CTAACTCACC TTCTCTTCTC GCGCTGCGTC ATGTGATGGA TGATGGAACC 
AACGAATACA AGATCATAAT GTTAAATAAA CGTTATCTTA GTTTTAGAGT CATTAAGGTA 
AACAAGGAGT GTGTGCGGGG CTTATGGGCT GGTCAGCAGC AAGAATTGGT CTTTCTTCGT 
AATCGGAACC CTGAAAGAGG CAGCATCCAA AATGCAAAGC AGGCTCTCAG GAATATGATC 
AATTCATCAT GTGACCAACC TATTGGATAC CCAATTTATG TTTCTCCACT GACAACCTCT 
TATTCAGACA GCCATGATCA GCTTAAACAG ATATTTGGTG GGCCAATTAG CTTGGCCAAT 
ATCCGGAACT TCATTGTATC TACATGGCAC AGATTAAGAA AAGGTTGCGG TGCAGGATGC 
AATAGTGGTG GAAATATTGA GGACTCTGAC ACTGGAGGCG GGACCTCCAT TAATAGCAAC 
AATCCACCAA ATGCAAATGA AACTCAGAGC AGTCTGTCAC AAGTTGGTGT CCCAGGTGGA 
ACAGGGCATG TGCCGAATGC ACGGCTCCAG CCTCCGGGGA TGCCTCATCC ATCAACACTA 
GGAACCAGTC ACAGTTCTCA CTCCATGCAG TCAAGCCTTG TCAGACATTC CCCAGCTCGT 
GGATCAGTAG CAAGTCAATC ATCTTACTGC TACAACAGCC GTCACTCATC ACTTAGAACA 
TCCACAACAG GATTTGTGCC TTGTAGGCGC TCCTCTACCA GCCAGATATC TCTTCGCAAT 
CTCCCCTCAT CCATTCAGTC CCGTCTTTCC ATGGTGAACC AAACAGACCC CACAGGACAG 
TCAGTTTTGG TTGGCCTTCC TTCCTCCAGC AGCTCAAGCC AGAGCATCCC TGCATGCAAA 
AGACACACCT TAGTGGGCTT TTTAGGAACA GAAGGAGGCA ATAACCTCAC TGAAACGCAA 
TCCTGTGGCA ATTCTAGTCC AGCAACCCAT TCAAGAGCCA AAAAGGAAGA GGTTGTTTAC 
AGAGTCCAGA TTGTGGATCC AAGCCAAGTG CTTGAAGGCG TGAATATATC CAAAAGAAAG 
GAGCTGCAGT GGCCAGATGA GCTGGTGCGG CTAAAAGCCG GCAAGAACAG CTGGAAAGAC 
TGGAGCCCTC AGGAAGGCAT GGAAGGCCAT GTAATTCACC GCTGGGTGCC TTGCAGCAGA 
GACCCTTGTA ATAGGTCCCA CATTGACAAA ACAATCCTGC TGATTAAGAT TGAGGACAAA 
TATGTTGCTG TGATTGAAAC TGGTGTGCTT GAACTCGGAG CTGAAGTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004917309.2
CDS	277..7287
Translation	MGSQTLQILRQGVWASITGGWYYDPHQSTFVNTLHLYIWLFLLCFPFTLYMALPTSLVIVAVYCPVVAAVFIVLKLVNYRLHRALDEGEIVERSASELTERVAKAEHSDSSARREDSNGPGDPGGGIEMSEFVREATPPVGCSSRNSYSGLDPSNQVGVSRYGGASGSKGDTDAAKTSDDISLSLGQSYSLCKEASEDQDLTVDQKLFRLVSNDSFVSMQPSISSGGKDLTRDFSDKGSIPTHSHHHHVDLSLSNACDTELASLVPLHSHSYRKDHRPRGVPRTSSSAVAFPDTSLTDFSLYQPRRGLDPVSELEASKPHSGSKDSLVDNACASGIFQLDCLKSKGAQQLSRSGSVKHMKTDKSMDSLRSLSTRSSGSTESYCSGTDRDTNSTVSSYKSEQTSSTHVESILSEHEDVTAAKRVHCSDLEEEHSSATDKRTSSIRTALERNTAQEAKDANTSDDVQSPRGLSSSASEEANKNPHANELTVQTDWLPGKLSVNIDEPGEKPVILVDLRSLEETGIKQRDNDVRPKSSSLIHRTSSAHKSCRRRTGKKRASSFDASRHKEYVSFQGIADTKPHSTGFCHDEDSSDQSDLSRTSSVQSAHRFSSDSSSSTTSHSCQSPEARYSALRTKYRIKERGTDSHHTHLNCEGTSKKRSNRRTSSASGTKSRARVLSLDSGTVVSLSDANRLAAPENIKTLTTSKSDLEAKEGEVLDQMSLLGRASQLESVTRSRNSLPSQVTFQDVGERDASVGAHASEETVTFRRERSTFRRQAVRRRHNAGSNPTPPASLIGSPLSLQDGHQGQQSSTQVKVQSRPPSQAAVLSASASLLVRNGSVHLEASHDNASAVGSSSLHDELGMQALCNLGDMEVLMQHILGKFTPSLYETGGCDMSLVNFEPAARRTSNNLCDTDSHVSSSTSVRFYPHDLIRLNRLLTIDTDLLEQQDIDLSPDLQDQMQPQEQAAQKVKHYYRFWILPKLWVGINFDRLTLLALFDRNREVLENVLSVILAILVAFLGSVLLIEGFFKDIWVFQFCLVIASCQYSLLKSVQPDSSSPRHGHNRIIAYSRPVYFCACCGLIWLLDYGSKNMSTTKYKLYGMAFTNPLLLVSARDLVIVFTLCFPVVFFIGLLPQVNTFVMYLCEQVDIHVFGGNATTSLLAAIYSFIRSIVAIALLYGLCYGALKDPWDAQHIPVLFSIFCGVLVAVSYHLSRQSSDPSVLISLVQSKIFPNSEDTNPEDPLSEVKDPLPEKLRSSVNERLQSDVVVCVVIAVLYFAIHVSTVFTALQPVLSYVLYALVGSVGLLTHYILPQVRKQLPWHCFSHPLLKTKEYYQFEVRNAAHLMWFEKAHIWLLFIEKNIIYPLIVLNELSIGAKDIVSPKKLDAEVGALLITIAGMKLLRSSFSSPTCQYATVIFTVLFFTFDYKEYTEKLLLDLFFMSILFSKLWELFFKLRFVYTYIAPWQITWGSAFHAFAQPFAVPHSAMLFVQAIVSALFSTPLNPFLGSAIFITSYVRPVKFWERDYNTKRVDHSNTRLASQLDRNPGSDDNNLNSIFYEHLTRSLQHSLCGDLLLGRWGNYNTGDCFILASDYLNALVHLIEIGNGLVTFQLRGLEFRGTYCQQREVEAITEGVEEDEGFCCCEPGHIPHMLSFNAAFGQRWLAWEVLVTKYVLEGYSITDNSAASMLQVFDLRRILTTYYVKGIIYYVTASAKLEEWLANETMQEGLRQCSDHCYVDVDPTFNPNIDEDYDLRLAGISRESFCASYLNWIKYCSSRREKPLDAEKDSPLVTLCYGLCILGRRALGTASHHMSSNLESFLYGLHALFKGDFRISSLRDEWIFADMELLRKVVVPGIRMSLKLHQDHFTSPDEYDDPAVLYEAITSHEQNLVIAHEGDPAWRSAVLSNSPSLLALRHVMDDGTNEYKIIMLNKRYLSFRVIKVNKECVRGLWAGQQQELVFLRNRNPERGSIQNAKQALRNMINSSCDQPIGYPIYVSPLTTSYSDSHDQLKQIFGGPISLANIRNFIVSTWHRLRKGCGAGCNSGGNIEDSDTGGGTSINSNNPPNANETQSSLSQVGVPGGTGHVPNARLQPPGMPHPSTLGTSHSSHSMQSSLVRHSPARGSVASQSSYCYNSRHSSLRTSTTGFVPCRRSSTSQISLRNLPSSIQSRLSMVNQTDPTGQSVLVGLPSSSSSSQSIPACKRHTLVGFLGTEGGNNLTETQSCGNSSPATHSRAKKEEVVYRVQIVDPSQVLEGVNISKRKELQWPDELVRLKAGKNSWKDWSPQEGMEGHVIHRWVPCSRDPCNRSHIDKTILLIKIEDKYVAVIETGVLELGAEV

Target ORF information:

RefSeq Version	XM_004917252.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pecanex 1 (pcnx1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004917252.4

ORF Insert Sequence:

ATGGGTTCCC AGACACTGCA AATCCTCCGG CAGGGAGTCT GGGCTTCCAT CACCGGAGGC 
TGGTACTACG ACCCCCACCA GAGTACCTTT GTCAATACCC TACACCTCTA CATCTGGCTC 
TTCCTCCTCT GCTTCCCCTT CACCCTCTAC ATGGCACTAC CCACATCTTT GGTGATTGTT 
GCTGTGTACT GCCCAGTCGT TGCAGCTGTT TTTATTGTTT TGAAGCTTGT TAACTACCGT 
CTGCACAGGG CATTGGATGA AGGAGAAATT GTGGAGAGGA GTGCTAGTGA GTTGACTGAG 
AGGGTGGCAA AGGCAGAACA CAGTGACAGC TCTGCAAGAA GAGAGGACAG CAATGGACCT 
GGTGATCCAG GTGGAGGGAT TGAAATGTCT GAATTTGTTC GAGAAGCAAC TCCTCCAGTT 
GGATGCAGTT CACGTAATTC CTACTCTGGG TTGGACCCAA GCAATCAGGT TGGTGTGTCT 
CGTTATGGAG GTGCATCTGG AAGCAAAGGG GATACAGATG CTGCAAAGAC ATCAGATGAT 
ATCAGTCTGA GTCTTGGCCA GAGTTACAGT TTGTGCAAAG AAGCAAGTGA AGACCAAGAT 
TTGACTGTGG ATCAGAAGCT TTTCCGTCTC GTCTCCAATG ACTCCTTTGT CTCCATGCAG 
CCATCTATAT CCTCGGGTGG AAAAGACTTA ACTAGAGACT TCAGTGACAA AGGATCAATC 
CCAACACACA GTCATCATCA CCATGTGGAT CTGTCTCTGT CTAATGCTTG TGACACAGAA 
TTGGCCTCAC TTGTTCCATT GCATTCACAT TCGTACAGGA AAGATCATCG ACCAAGAGGT 
GTCCCAAGGA CTTCAAGTTC TGCTGTTGCT TTCCCAGACA CTTCTCTCAC TGACTTTTCA 
CTCTATCAAC CAAGGCGCGG GCTGGATCCT GTTAGTGAAC TTGAAGCTTC CAAACCACAT 
TCTGGGTCCA AAGATTCACT AGTAGATAAT GCTTGTGCTT CAGGGATTTT TCAATTAGAC 
TGTCTAAAAA GCAAAGGAGC CCAGCAGCTT TCAAGAAGTG GTAGTGTTAA ACACATGAAG 
ACTGACAAAA GCATGGATAG TTTAAGAAGC CTCAGCACGA GAAGCAGTGG ATCCACAGAA 
AGTTATTGTA GTGGAACAGA TCGGGACACA AATAGCACTG TTAGCAGCTA CAAAAGTGAA 
CAGACCAGTT CAACTCATGT TGAAAGTATT TTATCTGAGC ATGAGGATGT TACTGCTGCA 
AAGAGAGTCC ATTGTAGTGA CCTGGAGGAA GAACATAGCT CTGCTACTGA TAAAAGGACT 
AGTAGTATAA GAACTGCTTT AGAAAGAAAC ACTGCTCAGG AAGCCAAGGA TGCCAACACA 
TCTGATGATG TACAAAGTCC AAGGGGTCTT AGCTCATCAG CATCAGAGGA AGCTAATAAG 
AATCCACACG CAAATGAGTT AACTGTACAA ACAGACTGGC TTCCAGGTAA ACTGTCAGTG 
AACATTGATG AACCTGGGGA GAAACCAGTC ATTTTAGTTG ACTTGAGATC TTTGGAAGAA 
ACGGGTATTA AGCAGAGAGA CAATGACGTA CGACCCAAAT CATCTAGTTT AATACATCGA 
ACATCCTCTG CTCATAAATC ATGCAGGAGA CGGACAGGTA AAAAAAGAGC CAGCAGTTTT 
GATGCAAGTA GACACAAAGA ATATGTTTCC TTTCAAGGTA TAGCTGATAC CAAACCTCAC 
AGTACTGGCT TTTGTCATGA TGAAGATTCT AGCGACCAAA GCGACTTGAG CAGGACCTCA 
AGCGTTCAGT CGGCCCACCG TTTCAGCAGC GATAGCTCTT CCAGCACTAC TTCCCATTCA 
TGCCAGTCTC CTGAAGCAAG ATACAGTGCC CTAAGGACAA AATATCGAAT AAAAGAAAGA 
GGCACAGATT CACATCACAC ACACCTAAAT TGTGAAGGAA CTAGTAAAAA GCGGTCCAAC 
CGCAGGACTT CTAGTGCAAG TGGTACAAAA AGCCGAGCTA GAGTATTAAG TCTGGACAGT 
GGCACTGTAG TTAGTTTGAG TGATGCCAAC AGACTGGCCG CTCCTGAAAA TATAAAAACC 
TTAACCACTT CAAAATCAGA TCTAGAAGCC AAAGAAGGCG AAGTGCTAGA TCAGATGTCT 
CTGTTGGGGC GAGCTTCACA GTTAGAATCT GTAACACGGT CAAGAAATAG TTTGCCCAGC 
CAGGTTACTT TCCAAGATGT TGGAGAGCGA GACGCATCTG TTGGAGCACA CGCCAGTGAG 
GAGACAGTAA CATTTCGCCG CGAACGCAGC ACATTTAGGC GCCAAGCGGT TCGGCGCAGA 
CACAATGCAG GGAGTAATCC CACCCCTCCT GCGTCACTCA TCGGATCACC TCTGAGCCTT 
CAAGATGGTC ATCAAGGCCA GCAGTCATCA ACCCAGGTCA AAGTACAGTC CCGCCCCCCT 
TCCCAGGCTG CAGTGCTCAG CGCTAGCGCC TCCTTGCTGG TGAGAAATGG GAGTGTCCAC 
TTAGAAGCGT CTCATGACAA TGCATCTGCC GTAGGCAGTA GCAGTTTGCA CGATGAACTT 
GGTATGCAGG CCTTATGTAA CCTTGGTGAC ATGGAAGTTT TAATGCAGCA CATTTTGGGT 
AAATTTACCC CATCTCTATA TGAGACTGGA GGTTGTGACA TGTCACTTGT AAACTTTGAG 
CCAGCTGCAA GAAGGACGTC TAATAATCTT TGTGACACAG ATTCCCATGT ATCAAGTTCT 
ACCTCAGTTC GATTCTACCC ACATGATCTA ATCCGATTAA ACAGGCTGCT AACCATAGAT 
ACAGATCTGT TAGAGCAGCA GGATATTGAT CTAAGCCCTG ACCTGCAGGA CCAGATGCAA 
CCACAGGAAC AGGCTGCCCA AAAAGTAAAA CACTACTACC GGTTTTGGAT TCTCCCAAAG 
CTGTGGGTCG GCATCAACTT TGACAGGCTT ACACTCTTAG CCTTGTTCGA TAGGAACCGC 
GAGGTCCTAG AAAATGTGCT GTCTGTCATT CTCGCCATTC TAGTTGCCTT TTTGGGCTCT 
GTTCTTCTCA TAGAAGGCTT CTTTAAAGAT ATCTGGGTCT TCCAGTTTTG TCTTGTTATA 
GCCAGCTGTC AATACTCTCT CCTTAAGAGT GTTCAACCAG ATTCTTCTTC CCCAAGACAT 
GGCCATAATC GTATCATTGC CTATAGTAGG CCCGTTTACT TCTGTGCCTG TTGCGGCCTT 
ATTTGGTTAT TGGATTATGG AAGCAAAAAC ATGTCTACCA CCAAATACAA ACTATATGGA 
ATGGCCTTCA CCAATCCTCT GCTTCTAGTG TCAGCCAGGG ACTTAGTCAT TGTCTTCACA 
CTCTGTTTCC CAGTCGTGTT CTTTATTGGT CTCCTGCCTC AGGTGAACAC ATTCGTCATG 
TACCTGTGTG AGCAAGTAGA TATACATGTG TTTGGTGGAA ATGCTACCAC CAGCTTGCTT 
GCAGCAATTT ACAGCTTTAT CCGTAGCATT GTGGCCATAG CTTTACTCTA CGGGCTGTGT 
TACGGTGCTC TTAAGGATCC ATGGGATGCA CAACACATTC CTGTATTATT CTCTATTTTC 
TGTGGTGTTC TTGTGGCAGT TTCATACCAT CTCAGCCGAC AGAGCAGCGA TCCCTCTGTC 
CTCATCTCAC TGGTGCAATC AAAGATTTTT CCAAACAGCG AGGATACAAA TCCTGAAGAC 
CCACTATCAG AAGTGAAAGA CCCTCTTCCA GAAAAACTGA GAAGCTCTGT TAATGAACGA 
CTTCAGTCAG ACGTAGTAGT CTGTGTCGTT ATTGCAGTGT TGTACTTTGC AATTCATGTC 
AGCACCGTAT TTACTGCATT ACAGCCGGTT CTGAGCTACG TACTCTATGC ACTAGTTGGT 
TCTGTTGGAC TCCTTACTCA CTACATTCTA CCACAAGTAC GGAAACAGCT TCCTTGGCAC 
TGCTTCTCCC ACCCGCTGCT GAAAACTAAG GAATATTACC AATTTGAAGT TCGAAATGCT 
GCTCATCTGA TGTGGTTTGA GAAGGCTCAC ATCTGGCTGC TTTTTATCGA GAAGAACATT 
ATTTATCCTC TTATAGTTCT CAATGAGCTT AGCATTGGTG CTAAGGATAT CGTCAGCCCG 
AAAAAGCTGG ATGCAGAAGT TGGTGCATTG TTGATCACCA TTGCCGGAAT GAAGCTGCTG 
AGGTCGTCAT TTAGCAGTCC CACCTGCCAG TATGCCACTG TCATTTTCAC TGTCCTCTTC 
TTTACTTTTG ACTACAAGGA GTATACAGAA AAACTGCTGC TAGATCTGTT CTTTATGTCA 
ATCCTCTTTA GCAAGCTCTG GGAACTGTTT TTCAAACTGC GCTTTGTCTA CACCTATATT 
GCCCCTTGGC AGATCACGTG GGGATCAGCA TTCCATGCTT TCGCACAGCC ATTTGCTGTA 
CCTCATTCTG CAATGCTGTT TGTACAGGCA ATTGTTTCTG CACTGTTCTC CACTCCCCTG 
AACCCTTTCT TAGGGAGTGC AATCTTTATT ACATCATATG TACGCCCTGT CAAGTTTTGG 
GAGAGAGACT ACAATACAAA GAGAGTTGAC CACTCAAACA CAAGATTAGC CTCTCAGCTT 
GACAGAAATC CAGGTTCAGA TGACAACAAC TTGAATTCCA TATTTTATGA GCATCTCACA 
CGCTCCCTTC AGCATAGTCT GTGTGGTGAT CTGTTGCTTG GCCGCTGGGG AAATTACAAT 
ACTGGGGATT GCTTTATTCT GGCCTCAGAT TATCTAAACG CCTTGGTGCA CCTTATCGAA 
ATAGGCAATG GTCTGGTCAC CTTCCAGCTA AGAGGACTAG AGTTCAGAGG TACCTACTGC 
CAGCAGCGTG AAGTTGAAGC AATCACTGAG GGTGTAGAGG AAGATGAAGG CTTTTGCTGT 
TGCGAACCTG GACATATTCC TCATATGCTG TCTTTCAATG CTGCCTTTGG GCAGCGCTGG 
CTGGCATGGG AAGTCTTGGT TACAAAGTAT GTTCTGGAAG GTTACAGCAT CACAGACAAC 
AGTGCAGCCT CTATGCTTCA AGTGTTTGAC CTTCGCAGGA TCCTCACTAC CTATTATGTT 
AAGGGCATTA TATACTATGT GACAGCATCT GCAAAACTGG AGGAGTGGTT AGCAAATGAG 
ACAATGCAGG AAGGTCTTCG CCAGTGTTCT GATCATTGTT ATGTTGATGT AGATCCAACT 
TTCAACCCAA ATATTGATGA AGACTATGAC CTTCGCTTAG CAGGCATTTC ACGGGAGAGC 
TTCTGTGCAA GCTATCTTAA CTGGATTAAG TACTGTTCCT CTAGAAGGGA GAAGCCTCTT 
GATGCTGAAA AGGATTCACC TTTGGTAACA CTTTGTTATG GGCTCTGTAT TTTGGGGAGA 
AGGGCACTTG GTACAGCTTC TCATCACATG TCCAGCAATT TGGAGTCCTT TCTATATGGT 
TTACATGCCT TGTTTAAAGG AGATTTCCGC ATATCATCTC TCCGTGATGA GTGGATTTTT 
GCTGATATGG AGCTTTTAAG AAAAGTAGTA GTTCCTGGCA TACGTATGTC ACTAAAACTT 
CATCAGGACC ACTTTACATC TCCAGATGAG TATGATGATC CTGCAGTTTT GTATGAAGCC 
ATTACATCAC ATGAGCAGAA CCTTGTTATT GCGCATGAGG GAGATCCAGC GTGGAGGAGT 
GCTGTTCTTT CTAACTCACC TTCTCTTCTC GCGCTGCGTC ATGTGATGGA TGATGGAACC 
AACGAATACA AGATCATAAT GTTAAATAAA CGTTATCTTA GTTTTAGAGT CATTAAGGTA 
AACAAGGAGT GTGTGCGGGG CTTATGGGCT GGTCAGCAGC AAGAATTGGT CTTTCTTCGT 
AATCGGAACC CTGAAAGAGG CAGCATCCAA AATGCAAAGC AGGCTCTCAG GAATATGATC 
AATTCATCAT GTGACCAACC TATTGGATAC CCAATTTATG TTTCTCCACT GACAACCTCT 
TATTCAGACA GCCATGATCA GCTTAAACAG ATATTTGGTG GGCCAATTAG CTTGGCCAAT 
ATCCGGAACT TCATTGTATC TACATGGCAC AGATTAAGAA AAGGTTGCGG TGCAGGATGC 
AATAGTGGTG GAAATATTGA GGACTCTGAC ACTGGAGGCG GGACCTCCAT TAATAGCAAC 
AATCCACCAA ATGCAAATGA AACTCAGAGC AGTCTGTCAC AAGTTGGTGT CCCAGGTGGA 
ACAGGGCATG TGCCGAATGC ACGGCTCCAG CCTCCGGGGA TGCCTCATCC ATCAACACTA 
GGAACCAGTC ACAGTTCTCA CTCCATGCAG TCAAGCCTTG TCAGACATTC CCCAGCTCGT 
GGATCAGTAG CAAGTCAATC ATCTTACTGC TACAACAGCC GTCACTCATC ACTTAGAACA 
TCCACAACAG GATTTGTGCC TTGTAGGCGC TCCTCTACCA GCCAGATATC TCTTCGCAAT 
CTCCCCTCAT CCATTCAGTC CCGTCTTTCC ATGGTGAACC AAACAGACCC CACAGGACAG 
TCAGTTTTGG TTGGCCTTCC TTCCTCCAGC AGCTCAAGCC AGAGCATCCC TGCATGCAAA 
AGACACACCT TAGTGGGCTT TTTAGGAACA GAAGGAGGCA ATAACCTCAC TGAAACGCAA 
TCCTGTGGCA ATTCTAGTCC AGCAACCCAT TCAAGAGCCA AAAAGGAAGA GGTTGTTTAC 
AGAGTCCAGA TTGTGGATCC AAGCCAAGTG CTTGAAGGCG TGAATATATC CAAAAGAAAG 
GAGCTGCAGT GGCCAGATGA GCTGGTGCGG CTAAAAGCCG GCAAGAACAG CTGGAAAGAC 
TGGAGCCCTC AGGAAGGCAT GGAAGGCCAT GTAATTCACC GCTGGGTGCC TTGCAGCAGA 
GACCCTTGTA ATAGGTCCCA CATTGACAAA ACAATCCTGC TGATTAAGAT TGAGGACAAA 
TATGTTGCTG TGATTGAAAC TGGTGTGCTT GAACTCGGAG CTGAAGTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pcnx1 ( XM_004917253.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004917310.2 Xenopus tropicalis pecanex 1 (pcnx1), transcript variant X3, mRNA.

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CloneID	OXa64209
Clone ID Related Accession (Same CDS sequence)	XM_004917253.4
Accession Version	XM_004917253.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6969bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pecanex-like protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004917253.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCC AGACACTGCA AATCCTCCGG CAGGGAGTCT GGGCTTCCAT CACCGGAGGC 
TGGTACTACG ACCCCCACCA GAGTACCTTT GTCAATACCC TACACCTCTA CATCTGGCTC 
TTCCTCCTCT GCTTCCCCTT CACCCTCTAC ATGGCACTAC CCACATCTTT GGTGATTGTT 
GCTGTGTACT GCCCAGTCGT TGCAGCTGTT TTTATTGTTT TGAAGCTTGT TAACTACCGT 
CTGCACAGGG CATTGGATGA AGGAGAAATT GTGGAGAGGA GTGCTAGTGA GTTGACTGAG 
AGGGTGGCAA AGGCAGAACA CAGTGACAGC TCTGCAAGAA GAGAGGACAG CAATGGACCT 
GGTGATCCAG GTGGAGGGAT TGAAATGTCT GAATTTGTTC GAGAAGCAAC TCCTCCAGTT 
GGATGCAGTT CACGTAATTC CTACTCTGGG TTGGACCCAA GCAATCAGGT TGGTGTGTCT 
CGTTATGGAG GTGCATCTGG AAGCAAAGGG GATACAGATG CTGCAAAGAC ATCAGATGAT 
ATCAGTCTGA GTCTTGGCCA GAGTTACAGT TTGTGCAAAG AAGCAAGTGA AGACCAAGAT 
TTGACTGTGG ATCAGAAGCT TTTCCGTCTC GTCTCCAATG ACTCCTTTGT CTCCATGCAG 
CCATCTATAT CCTCGGGTGG AAAAGACTTA ACTAGAGACT TCAGTGACAA AGGATCAATC 
CCAACACACA GTCATCATCA CCATGTGGAT CTGTCTCTGT CTAATGCTTG TGACACAGAA 
TTGGCCTCAC TTGTTCCATT GCATTCACAT TCGTACAGGA AAGATCATCG ACCAAGAGGT 
GTCCCAAGGA CTTCAAGTTC TGCTGTTGCT TTCCCAGACA CTTCTCTCAC TGACTTTTCA 
CTCTATCAAC CAAGGCGCGG GCTGGATCCT GTTAGTGAAC TTGAAGCTTC CAAACCACAT 
TCTGGGTCCA AAGATTCACT AGTAGATAAT GCTTGTGCTT CAGGGATTTT TCAATTAGAC 
TGTCTAAAAA GCAAAGGAGC CCAGCAGCTT TCAAGAAGTG GTAGTGTTAA ACACATGAAG 
ACTGACAAAA GCATGGATAG TTTAAGAAGC CTCAGCACGA GAAGCAGTGG ATCCACAGAA 
AGTTATTGTA GTGGAACAGA TCGGGACACA AATAGCACTG TTAGCAGCTA CAAAAGTGAA 
CAGACCAGTT CAACTCATGT TGAAAGTATT TTATCTGAGC ATGAGGATGT TACTGCTGCA 
AAGAGAGTCC ATTGTAGTGA CCTGGAGGAA GAACATAGCT CTGCTACTGA TAAAAGGACT 
AGTAGTATAA GAACTGCTTT AGAAAGAAAC ACTGCTCAGG AAGCCAAGGA TGCCAACACA 
TCTGATGATG TACAAAGTCC AAGGGGTCTT AGCTCATCAG CATCAGAGGA AGCTAATAAG 
AATCCACACG CAAATGAGTT AACTGTACAA ACAGACTGGC TTCCAGGTAA ACTGTCAGTG 
AACATTGATG AACCTGGGGA GAAACCAGTC ATTTTAGTTG ACTTGAGATC TTTGGAAGAA 
ACGGGTATTA AGCAGAGAGA CAATGACGTA CGACCCAAAT CATCTAGTTT AATACATCGA 
ACATCCTCTG CTCATAAATC ATGCAGGAGA CGGACAGGTA AAAAAAGAGC CAGCAGTTTT 
GATGCAAGTA GACACAAAGA ATATGTTTCC TTTCAAGGTA TAGCTGATAC CAAACCTCAC 
AGTACTGGCT TTTGTCATGA TGAAGATTCT AGCGACCAAA GCGACTTGAG CAGGACCTCA 
AGCGTTCAGT CGGCCCACCG TTTCAGCAGC GATAGCTCTT CCAGCACTAC TTCCCATTCA 
TGCCAGTCTC CTGAAGCAAG ATACAGTGCC CTAAGGACAA AATATCGAAT AAAAGAAAGA 
GGCACAGATT CACATCACAC ACACCTAAAT TGTGAAGGAA CTAGTAAAAA GCGGTCCAAC 
CGCAGGACTT CTAGTGCAAG TGGTACAAAA AGCCGAGCTA GAGTATTAAG TCTGGACAGT 
GGCACTGTAG TTAGTTTGAG TGATGCCAAC AGACTGGCCG CTCCTGAAAA TATAAAAACC 
TTAACCACTT CAAAATCAGA TCTAGAAGCC AAAGAAGGCG AAGTGCTAGA TCAGATGTCT 
CTGTTGGGGC GAGCTTCACA GTTAGAATCT GTAACACGGT CAAGAAATAG TTTGCCCAGC 
CAGGTTACTT TCCAAGATGT TGGAGAGCGA GACGCATCTG TTGGAGCACA CGCCAGTGAG 
GAGACAGTAA CATTTCGCCG CGAACGCAGC ACATTTAGGC GCCAAGCGGT TCGGCGCAGA 
CACAATGCAG GGAGTAATCC CACCCCTCCT GCGTCACTCA TCGGATCACC TCTGAGCCTT 
CAAGATGGTC ATCAAGGCCA GCAGTCATCA ACCCAGGTCA AAGTACAGTC CCGCCCCCCT 
TCCCAGGCTG CAGTGCTCAG CGCTAGCGCC TCCTTGCTGG TGAGAAATGG GAGTGTCCAC 
TTAGAAGCGT CTCATGACAA TGCATCTGCC GTAGGCAGTA GCAGTTTGCA CGATGAACTT 
GGTAAATTTA CCCCATCTCT ATATGAGACT GGAGGTTGTG ACATGTCACT TGTAAACTTT 
GAGCCAGCTG CAAGAAGGAC GTCTAATAAT CTTTGTGACA CAGATTCCCA TGTATCAAGT 
TCTACCTCAG TTCGATTCTA CCCACATGAT CTACTCTCTC TTCCCCAGAT CCGATTAAAC 
AGGCTGCTAA CCATAGATAC AGATCTGTTA GAGCAGCAGG ATATTGATCT AAGCCCTGAC 
CTGCAGGACC AGATGCAACC ACAGGAACAG GCTGCCCAAA AAGTAAAACA CTACTACCGG 
TTTTGGATTC TCCCAAAGCT GTGGGTCGGC ATCAACTTTG ACAGGCTTAC ACTCTTAGCC 
TTGTTCGATA GGAACCGCGA GGTCCTAGAA AATGTGCTGT CTGTCATTCT CGCCATTCTA 
GTTGCCTTTT TGGGCTCTGT TCTTCTCATA GAAGGCTTCT TTAAAGATAT CTGGGTCTTC 
CAGTTTTGTC TTGTTATAGC CAGCTGTCAA TACTCTCTCC TTAAGAGTGT TCAACCAGAT 
TCTTCTTCCC CAAGACATGG CCATAATCGT ATCATTGCCT ATAGTAGGCC CGTTTACTTC 
TGTGCCTGTT GCGGCCTTAT TTGGTTATTG GATTATGGAA GCAAAAACAT GTCTACCACC 
AAATACAAAC TATATGGAAT GGCCTTCACC AATCCTCTGC TTCTAGTGTC AGCCAGGGAC 
TTAGTCATTG TCTTCACACT CTGTTTCCCA GTCGTGTTCT TTATTGGTCT CCTGCCTCAG 
GTGAACACAT TCGTCATGTA CCTGTGTGAG CAAGTAGATA TACATGTGTT TGGTGGAAAT 
GCTACCACCA GCTTGCTTGC AGCAATTTAC AGCTTTATCC GTAGCATTGT GGCCATAGCT 
TTACTCTACG GGCTGTGTTA CGGTGCTCTT AAGGATCCAT GGGATGCACA ACACATTCCT 
GTATTATTCT CTATTTTCTG TGGTGTTCTT GTGGCAGTTT CATACCATCT CAGCCGACAG 
AGCAGCGATC CCTCTGTCCT CATCTCACTG GTGCAATCAA AGATTTTTCC AAACAGCGAG 
GATACAAATC CTGAAGACCC ACTATCAGAA GTGAAAGACC CTCTTCCAGA AAAACTGAGA 
AGCTCTGTTA ATGAACGACT TCAGTCAGAC GTAGTAGTCT GTGTCGTTAT TGCAGTGTTG 
TACTTTGCAA TTCATGTCAG CACCGTATTT ACTGCATTAC AGCCGGTTCT GAGCTACGTA 
CTCTATGCAC TAGTTGGTTC TGTTGGACTC CTTACTCACT ACATTCTACC ACAAGTACGG 
AAACAGCTTC CTTGGCACTG CTTCTCCCAC CCGCTGCTGA AAACTAAGGA ATATTACCAA 
TTTGAAGTTC GAAATGCTGC TCATCTGATG TGGTTTGAGA AGGCTCACAT CTGGCTGCTT 
TTTATCGAGA AGAACATTAT TTATCCTCTT ATAGTTCTCA ATGAGCTTAG CATTGGTGCT 
AAGGATATCG TCAGCCCGAA AAAGCTGGAT GCAGAAGTTG GTGCATTGTT GATCACCATT 
GCCGGAATGA AGCTGCTGAG GTCGTCATTT AGCAGTCCCA CCTGCCAGTA TGCCACTGTC 
ATTTTCACTG TCCTCTTCTT TACTTTTGAC TACAAGGAGT ATACAGAAAA ACTGCTGCTA 
GATCTGTTCT TTATGTCAAT CCTCTTTAGC AAGCTCTGGG AACTGTTTTT CAAACTGCGC 
TTTGTCTACA CCTATATTGC CCCTTGGCAG ATCACGTGGG GATCAGCATT CCATGCTTTC 
GCACAGCCAT TTGCTGTACC TCATTCTGCA ATGCTGTTTG TACAGGCAAT TGTTTCTGCA 
CTGTTCTCCA CTCCCCTGAA CCCTTTCTTA GGGAGTGCAA TCTTTATTAC ATCATATGTA 
CGCCCTGTCA AGTTTTGGGA GAGAGACTAC AATACAAAGA GAGTTGACCA CTCAAACACA 
AGATTAGCCT CTCAGCTTGA CAGAAATCCA GGTTCAGATG ACAACAACTT GAATTCCATA 
TTTTATGAGC ATCTCACACG CTCCCTTCAG CATAGTCTGT GTGGTGATCT GTTGCTTGGC 
CGCTGGGGAA ATTACAATAC TGGGGATTGC TTTATTCTGG CCTCAGATTA TCTAAACGCC 
TTGGTGCACC TTATCGAAAT AGGCAATGGT CTGGTCACCT TCCAGCTAAG AGGACTAGAG 
TTCAGAGGTA CCTACTGCCA GCAGCGTGAA GTTGAAGCAA TCACTGAGGG TGTAGAGGAA 
GATGAAGGCT TTTGCTGTTG CGAACCTGGA CATATTCCTC ATATGCTGTC TTTCAATGCT 
GCCTTTGGGC AGCGCTGGCT GGCATGGGAA GTCTTGGTTA CAAAGTATGT TCTGGAAGGT 
TACAGCATCA CAGACAACAG TGCAGCCTCT ATGCTTCAAG TGTTTGACCT TCGCAGGATC 
CTCACTACCT ATTATGTTAA GGGCATTATA TACTATGTGA CAGCATCTGC AAAACTGGAG 
GAGTGGTTAG CAAATGAGAC AATGCAGGAA GGTCTTCGCC AGTGTTCTGA TCATTGTTAT 
GTTGATGTAG ATCCAACTTT CAACCCAAAT ATTGATGAAG ACTATGACCT TCGCTTAGCA 
GGCATTTCAC GGGAGAGCTT CTGTGCAAGC TATCTTAACT GGATTAAGTA CTGTTCCTCT 
AGAAGGGAGA AGCCTCTTGA TGCTGAAAAG GATTCACCTT TGGTAACACT TTGTTATGGG 
CTCTGTATTT TGGGGAGAAG GGCACTTGGT ACAGCTTCTC ATCACATGTC CAGCAATTTG 
GAGTCCTTTC TATATGGTTT ACATGCCTTG TTTAAAGGAG ATTTCCGCAT ATCATCTCTC 
CGTGATGAGT GGATTTTTGC TGATATGGAG CTTTTAAGAA AAGTAGTAGT TCCTGGCATA 
CGTATGTCAC TAAAACTTCA TCAGGACCAC TTTACATCTC CAGATGAGTA TGATGATCCT 
GCAGTTTTGT ATGAAGCCAT TACATCACAT GAGCAGAACC TTGTTATTGC GCATGAGGGA 
GATCCAGCGT GGAGGAGTGC TGTTCTTTCT AACTCACCTT CTCTTCTCGC GCTGCGTCAT 
GTGATGGATG ATGGAACCAA CGAATACAAG ATCATAATGT TAAATAAACG TTATCTTAGT 
TTTAGAGTCA TTAAGGTAAA CAAGGAGTGT GTGCGGGGCT TATGGGCTGG TCAGCAGCAA 
GAATTGGTCT TTCTTCGTAA TCGGAACCCT GAAAGAGGCA GCATCCAAAA TGCAAAGCAG 
GCTCTCAGGA ATATGATCAA TTCATCATGT GACCAACCTA TTGGATACCC AATTTATGTT 
TCTCCACTGA CAACCTCTTA TTCAGACAGC CATGATCAGC TTAAACAGAT ATTTGGTGGG 
CCAATTAGCT TGGCCAATAT CCGGAACTTC ATTGTATCTA CATGGCACAG ATTAAGAAAA 
GGTTGCGGTG CAGGATGCAA TAGTGGTGGA AATATTGAGG ACTCTGACAC TGGAGGCGGG 
ACCTCCATTA ATAGCAACAA TCCACCAAAT GCAAATGAAA CTCAGAGCAG TCTGTCACAA 
GTTGGTGTCC CAGGTGGAAC AGGGCATGTG CCGAATGCAC GGCTCCAGCC TCCGGGGATG 
CCTCATCCAT CAACACTAGG AACCAGTCAC AGTTCTCACT CCATGCAGTC AAGCCTTGTC 
AGACATTCCC CAGCTCGTGG ATCAGTAGCA AGTCAATCAT CTTACTGCTA CAACAGCCGT 
CACTCATCAC TTAGAACATC CACAACAGGA TTTGTGCCTT GTAGGCGCTC CTCTACCAGC 
CAGATATCTC TTCGCAATCT CCCCTCATCC ATTCAGTCCC GTCTTTCCAT GGTGAACCAA 
ACAGACCCCA CAGGACAGTC AGTTTTGGTT GGCCTTCCTT CCTCCAGCAG CTCAAGCCAG 
AGCATCCCTG CATGCAAAAG ACACACCTTA GTGGGCTTTT TAGGAACAGA AGGAGGCAAT 
AACCTCACTG AAACGCAATC CTGTGGCAAT TCTAGTCCAG CAACCCATTC AAGAGCCAAA 
AAGGAAGAGG TTGTTTACAG AGTCCAGATT GTGGATCCAA GCCAAGTGCT TGAAGGCGTG 
AATATATCCA AAAGAAAGGA GCTGCAGTGG CCAGATGAGC TGGTGCGGCT AAAAGCCGGC 
AAGAACAGCT GGAAAGACTG GAGCCCTCAG GAAGGCATGG AAGGCCATGT AATTCACCGC 
TGGGTGCCTT GCAGCAGAGA CCCTTGTAAT AGGTCCCACA TTGACAAAAC AATCCTGCTG 
ATTAAGATTG AGGACAAATA TGTTGCTGTG ATTGAAACTG GTGTGCTTGA ACTCGGAGCT 
GAAGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004917310.2
CDS	277..7245
Translation	MGSQTLQILRQGVWASITGGWYYDPHQSTFVNTLHLYIWLFLLCFPFTLYMALPTSLVIVAVYCPVVAAVFIVLKLVNYRLHRALDEGEIVERSASELTERVAKAEHSDSSARREDSNGPGDPGGGIEMSEFVREATPPVGCSSRNSYSGLDPSNQVGVSRYGGASGSKGDTDAAKTSDDISLSLGQSYSLCKEASEDQDLTVDQKLFRLVSNDSFVSMQPSISSGGKDLTRDFSDKGSIPTHSHHHHVDLSLSNACDTELASLVPLHSHSYRKDHRPRGVPRTSSSAVAFPDTSLTDFSLYQPRRGLDPVSELEASKPHSGSKDSLVDNACASGIFQLDCLKSKGAQQLSRSGSVKHMKTDKSMDSLRSLSTRSSGSTESYCSGTDRDTNSTVSSYKSEQTSSTHVESILSEHEDVTAAKRVHCSDLEEEHSSATDKRTSSIRTALERNTAQEAKDANTSDDVQSPRGLSSSASEEANKNPHANELTVQTDWLPGKLSVNIDEPGEKPVILVDLRSLEETGIKQRDNDVRPKSSSLIHRTSSAHKSCRRRTGKKRASSFDASRHKEYVSFQGIADTKPHSTGFCHDEDSSDQSDLSRTSSVQSAHRFSSDSSSSTTSHSCQSPEARYSALRTKYRIKERGTDSHHTHLNCEGTSKKRSNRRTSSASGTKSRARVLSLDSGTVVSLSDANRLAAPENIKTLTTSKSDLEAKEGEVLDQMSLLGRASQLESVTRSRNSLPSQVTFQDVGERDASVGAHASEETVTFRRERSTFRRQAVRRRHNAGSNPTPPASLIGSPLSLQDGHQGQQSSTQVKVQSRPPSQAAVLSASASLLVRNGSVHLEASHDNASAVGSSSLHDELGKFTPSLYETGGCDMSLVNFEPAARRTSNNLCDTDSHVSSSTSVRFYPHDLLSLPQIRLNRLLTIDTDLLEQQDIDLSPDLQDQMQPQEQAAQKVKHYYRFWILPKLWVGINFDRLTLLALFDRNREVLENVLSVILAILVAFLGSVLLIEGFFKDIWVFQFCLVIASCQYSLLKSVQPDSSSPRHGHNRIIAYSRPVYFCACCGLIWLLDYGSKNMSTTKYKLYGMAFTNPLLLVSARDLVIVFTLCFPVVFFIGLLPQVNTFVMYLCEQVDIHVFGGNATTSLLAAIYSFIRSIVAIALLYGLCYGALKDPWDAQHIPVLFSIFCGVLVAVSYHLSRQSSDPSVLISLVQSKIFPNSEDTNPEDPLSEVKDPLPEKLRSSVNERLQSDVVVCVVIAVLYFAIHVSTVFTALQPVLSYVLYALVGSVGLLTHYILPQVRKQLPWHCFSHPLLKTKEYYQFEVRNAAHLMWFEKAHIWLLFIEKNIIYPLIVLNELSIGAKDIVSPKKLDAEVGALLITIAGMKLLRSSFSSPTCQYATVIFTVLFFTFDYKEYTEKLLLDLFFMSILFSKLWELFFKLRFVYTYIAPWQITWGSAFHAFAQPFAVPHSAMLFVQAIVSALFSTPLNPFLGSAIFITSYVRPVKFWERDYNTKRVDHSNTRLASQLDRNPGSDDNNLNSIFYEHLTRSLQHSLCGDLLLGRWGNYNTGDCFILASDYLNALVHLIEIGNGLVTFQLRGLEFRGTYCQQREVEAITEGVEEDEGFCCCEPGHIPHMLSFNAAFGQRWLAWEVLVTKYVLEGYSITDNSAASMLQVFDLRRILTTYYVKGIIYYVTASAKLEEWLANETMQEGLRQCSDHCYVDVDPTFNPNIDEDYDLRLAGISRESFCASYLNWIKYCSSRREKPLDAEKDSPLVTLCYGLCILGRRALGTASHHMSSNLESFLYGLHALFKGDFRISSLRDEWIFADMELLRKVVVPGIRMSLKLHQDHFTSPDEYDDPAVLYEAITSHEQNLVIAHEGDPAWRSAVLSNSPSLLALRHVMDDGTNEYKIIMLNKRYLSFRVIKVNKECVRGLWAGQQQELVFLRNRNPERGSIQNAKQALRNMINSSCDQPIGYPIYVSPLTTSYSDSHDQLKQIFGGPISLANIRNFIVSTWHRLRKGCGAGCNSGGNIEDSDTGGGTSINSNNPPNANETQSSLSQVGVPGGTGHVPNARLQPPGMPHPSTLGTSHSSHSMQSSLVRHSPARGSVASQSSYCYNSRHSSLRTSTTGFVPCRRSSTSQISLRNLPSSIQSRLSMVNQTDPTGQSVLVGLPSSSSSSQSIPACKRHTLVGFLGTEGGNNLTETQSCGNSSPATHSRAKKEEVVYRVQIVDPSQVLEGVNISKRKELQWPDELVRLKAGKNSWKDWSPQEGMEGHVIHRWVPCSRDPCNRSHIDKTILLIKIEDKYVAVIETGVLELGAEV

Target ORF information:

RefSeq Version	XM_004917253.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pecanex 1 (pcnx1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004917253.4

ORF Insert Sequence:

ATGGGTTCCC AGACACTGCA AATCCTCCGG CAGGGAGTCT GGGCTTCCAT CACCGGAGGC 
TGGTACTACG ACCCCCACCA GAGTACCTTT GTCAATACCC TACACCTCTA CATCTGGCTC 
TTCCTCCTCT GCTTCCCCTT CACCCTCTAC ATGGCACTAC CCACATCTTT GGTGATTGTT 
GCTGTGTACT GCCCAGTCGT TGCAGCTGTT TTTATTGTTT TGAAGCTTGT TAACTACCGT 
CTGCACAGGG CATTGGATGA AGGAGAAATT GTGGAGAGGA GTGCTAGTGA GTTGACTGAG 
AGGGTGGCAA AGGCAGAACA CAGTGACAGC TCTGCAAGAA GAGAGGACAG CAATGGACCT 
GGTGATCCAG GTGGAGGGAT TGAAATGTCT GAATTTGTTC GAGAAGCAAC TCCTCCAGTT 
GGATGCAGTT CACGTAATTC CTACTCTGGG TTGGACCCAA GCAATCAGGT TGGTGTGTCT 
CGTTATGGAG GTGCATCTGG AAGCAAAGGG GATACAGATG CTGCAAAGAC ATCAGATGAT 
ATCAGTCTGA GTCTTGGCCA GAGTTACAGT TTGTGCAAAG AAGCAAGTGA AGACCAAGAT 
TTGACTGTGG ATCAGAAGCT TTTCCGTCTC GTCTCCAATG ACTCCTTTGT CTCCATGCAG 
CCATCTATAT CCTCGGGTGG AAAAGACTTA ACTAGAGACT TCAGTGACAA AGGATCAATC 
CCAACACACA GTCATCATCA CCATGTGGAT CTGTCTCTGT CTAATGCTTG TGACACAGAA 
TTGGCCTCAC TTGTTCCATT GCATTCACAT TCGTACAGGA AAGATCATCG ACCAAGAGGT 
GTCCCAAGGA CTTCAAGTTC TGCTGTTGCT TTCCCAGACA CTTCTCTCAC TGACTTTTCA 
CTCTATCAAC CAAGGCGCGG GCTGGATCCT GTTAGTGAAC TTGAAGCTTC CAAACCACAT 
TCTGGGTCCA AAGATTCACT AGTAGATAAT GCTTGTGCTT CAGGGATTTT TCAATTAGAC 
TGTCTAAAAA GCAAAGGAGC CCAGCAGCTT TCAAGAAGTG GTAGTGTTAA ACACATGAAG 
ACTGACAAAA GCATGGATAG TTTAAGAAGC CTCAGCACGA GAAGCAGTGG ATCCACAGAA 
AGTTATTGTA GTGGAACAGA TCGGGACACA AATAGCACTG TTAGCAGCTA CAAAAGTGAA 
CAGACCAGTT CAACTCATGT TGAAAGTATT TTATCTGAGC ATGAGGATGT TACTGCTGCA 
AAGAGAGTCC ATTGTAGTGA CCTGGAGGAA GAACATAGCT CTGCTACTGA TAAAAGGACT 
AGTAGTATAA GAACTGCTTT AGAAAGAAAC ACTGCTCAGG AAGCCAAGGA TGCCAACACA 
TCTGATGATG TACAAAGTCC AAGGGGTCTT AGCTCATCAG CATCAGAGGA AGCTAATAAG 
AATCCACACG CAAATGAGTT AACTGTACAA ACAGACTGGC TTCCAGGTAA ACTGTCAGTG 
AACATTGATG AACCTGGGGA GAAACCAGTC ATTTTAGTTG ACTTGAGATC TTTGGAAGAA 
ACGGGTATTA AGCAGAGAGA CAATGACGTA CGACCCAAAT CATCTAGTTT AATACATCGA 
ACATCCTCTG CTCATAAATC ATGCAGGAGA CGGACAGGTA AAAAAAGAGC CAGCAGTTTT 
GATGCAAGTA GACACAAAGA ATATGTTTCC TTTCAAGGTA TAGCTGATAC CAAACCTCAC 
AGTACTGGCT TTTGTCATGA TGAAGATTCT AGCGACCAAA GCGACTTGAG CAGGACCTCA 
AGCGTTCAGT CGGCCCACCG TTTCAGCAGC GATAGCTCTT CCAGCACTAC TTCCCATTCA 
TGCCAGTCTC CTGAAGCAAG ATACAGTGCC CTAAGGACAA AATATCGAAT AAAAGAAAGA 
GGCACAGATT CACATCACAC ACACCTAAAT TGTGAAGGAA CTAGTAAAAA GCGGTCCAAC 
CGCAGGACTT CTAGTGCAAG TGGTACAAAA AGCCGAGCTA GAGTATTAAG TCTGGACAGT 
GGCACTGTAG TTAGTTTGAG TGATGCCAAC AGACTGGCCG CTCCTGAAAA TATAAAAACC 
TTAACCACTT CAAAATCAGA TCTAGAAGCC AAAGAAGGCG AAGTGCTAGA TCAGATGTCT 
CTGTTGGGGC GAGCTTCACA GTTAGAATCT GTAACACGGT CAAGAAATAG TTTGCCCAGC 
CAGGTTACTT TCCAAGATGT TGGAGAGCGA GACGCATCTG TTGGAGCACA CGCCAGTGAG 
GAGACAGTAA CATTTCGCCG CGAACGCAGC ACATTTAGGC GCCAAGCGGT TCGGCGCAGA 
CACAATGCAG GGAGTAATCC CACCCCTCCT GCGTCACTCA TCGGATCACC TCTGAGCCTT 
CAAGATGGTC ATCAAGGCCA GCAGTCATCA ACCCAGGTCA AAGTACAGTC CCGCCCCCCT 
TCCCAGGCTG CAGTGCTCAG CGCTAGCGCC TCCTTGCTGG TGAGAAATGG GAGTGTCCAC 
TTAGAAGCGT CTCATGACAA TGCATCTGCC GTAGGCAGTA GCAGTTTGCA CGATGAACTT 
GGTAAATTTA CCCCATCTCT ATATGAGACT GGAGGTTGTG ACATGTCACT TGTAAACTTT 
GAGCCAGCTG CAAGAAGGAC GTCTAATAAT CTTTGTGACA CAGATTCCCA TGTATCAAGT 
TCTACCTCAG TTCGATTCTA CCCACATGAT CTACTCTCTC TTCCCCAGAT CCGATTAAAC 
AGGCTGCTAA CCATAGATAC AGATCTGTTA GAGCAGCAGG ATATTGATCT AAGCCCTGAC 
CTGCAGGACC AGATGCAACC ACAGGAACAG GCTGCCCAAA AAGTAAAACA CTACTACCGG 
TTTTGGATTC TCCCAAAGCT GTGGGTCGGC ATCAACTTTG ACAGGCTTAC ACTCTTAGCC 
TTGTTCGATA GGAACCGCGA GGTCCTAGAA AATGTGCTGT CTGTCATTCT CGCCATTCTA 
GTTGCCTTTT TGGGCTCTGT TCTTCTCATA GAAGGCTTCT TTAAAGATAT CTGGGTCTTC 
CAGTTTTGTC TTGTTATAGC CAGCTGTCAA TACTCTCTCC TTAAGAGTGT TCAACCAGAT 
TCTTCTTCCC CAAGACATGG CCATAATCGT ATCATTGCCT ATAGTAGGCC CGTTTACTTC 
TGTGCCTGTT GCGGCCTTAT TTGGTTATTG GATTATGGAA GCAAAAACAT GTCTACCACC 
AAATACAAAC TATATGGAAT GGCCTTCACC AATCCTCTGC TTCTAGTGTC AGCCAGGGAC 
TTAGTCATTG TCTTCACACT CTGTTTCCCA GTCGTGTTCT TTATTGGTCT CCTGCCTCAG 
GTGAACACAT TCGTCATGTA CCTGTGTGAG CAAGTAGATA TACATGTGTT TGGTGGAAAT 
GCTACCACCA GCTTGCTTGC AGCAATTTAC AGCTTTATCC GTAGCATTGT GGCCATAGCT 
TTACTCTACG GGCTGTGTTA CGGTGCTCTT AAGGATCCAT GGGATGCACA ACACATTCCT 
GTATTATTCT CTATTTTCTG TGGTGTTCTT GTGGCAGTTT CATACCATCT CAGCCGACAG 
AGCAGCGATC CCTCTGTCCT CATCTCACTG GTGCAATCAA AGATTTTTCC AAACAGCGAG 
GATACAAATC CTGAAGACCC ACTATCAGAA GTGAAAGACC CTCTTCCAGA AAAACTGAGA 
AGCTCTGTTA ATGAACGACT TCAGTCAGAC GTAGTAGTCT GTGTCGTTAT TGCAGTGTTG 
TACTTTGCAA TTCATGTCAG CACCGTATTT ACTGCATTAC AGCCGGTTCT GAGCTACGTA 
CTCTATGCAC TAGTTGGTTC TGTTGGACTC CTTACTCACT ACATTCTACC ACAAGTACGG 
AAACAGCTTC CTTGGCACTG CTTCTCCCAC CCGCTGCTGA AAACTAAGGA ATATTACCAA 
TTTGAAGTTC GAAATGCTGC TCATCTGATG TGGTTTGAGA AGGCTCACAT CTGGCTGCTT 
TTTATCGAGA AGAACATTAT TTATCCTCTT ATAGTTCTCA ATGAGCTTAG CATTGGTGCT 
AAGGATATCG TCAGCCCGAA AAAGCTGGAT GCAGAAGTTG GTGCATTGTT GATCACCATT 
GCCGGAATGA AGCTGCTGAG GTCGTCATTT AGCAGTCCCA CCTGCCAGTA TGCCACTGTC 
ATTTTCACTG TCCTCTTCTT TACTTTTGAC TACAAGGAGT ATACAGAAAA ACTGCTGCTA 
GATCTGTTCT TTATGTCAAT CCTCTTTAGC AAGCTCTGGG AACTGTTTTT CAAACTGCGC 
TTTGTCTACA CCTATATTGC CCCTTGGCAG ATCACGTGGG GATCAGCATT CCATGCTTTC 
GCACAGCCAT TTGCTGTACC TCATTCTGCA ATGCTGTTTG TACAGGCAAT TGTTTCTGCA 
CTGTTCTCCA CTCCCCTGAA CCCTTTCTTA GGGAGTGCAA TCTTTATTAC ATCATATGTA 
CGCCCTGTCA AGTTTTGGGA GAGAGACTAC AATACAAAGA GAGTTGACCA CTCAAACACA 
AGATTAGCCT CTCAGCTTGA CAGAAATCCA GGTTCAGATG ACAACAACTT GAATTCCATA 
TTTTATGAGC ATCTCACACG CTCCCTTCAG CATAGTCTGT GTGGTGATCT GTTGCTTGGC 
CGCTGGGGAA ATTACAATAC TGGGGATTGC TTTATTCTGG CCTCAGATTA TCTAAACGCC 
TTGGTGCACC TTATCGAAAT AGGCAATGGT CTGGTCACCT TCCAGCTAAG AGGACTAGAG 
TTCAGAGGTA CCTACTGCCA GCAGCGTGAA GTTGAAGCAA TCACTGAGGG TGTAGAGGAA 
GATGAAGGCT TTTGCTGTTG CGAACCTGGA CATATTCCTC ATATGCTGTC TTTCAATGCT 
GCCTTTGGGC AGCGCTGGCT GGCATGGGAA GTCTTGGTTA CAAAGTATGT TCTGGAAGGT 
TACAGCATCA CAGACAACAG TGCAGCCTCT ATGCTTCAAG TGTTTGACCT TCGCAGGATC 
CTCACTACCT ATTATGTTAA GGGCATTATA TACTATGTGA CAGCATCTGC AAAACTGGAG 
GAGTGGTTAG CAAATGAGAC AATGCAGGAA GGTCTTCGCC AGTGTTCTGA TCATTGTTAT 
GTTGATGTAG ATCCAACTTT CAACCCAAAT ATTGATGAAG ACTATGACCT TCGCTTAGCA 
GGCATTTCAC GGGAGAGCTT CTGTGCAAGC TATCTTAACT GGATTAAGTA CTGTTCCTCT 
AGAAGGGAGA AGCCTCTTGA TGCTGAAAAG GATTCACCTT TGGTAACACT TTGTTATGGG 
CTCTGTATTT TGGGGAGAAG GGCACTTGGT ACAGCTTCTC ATCACATGTC CAGCAATTTG 
GAGTCCTTTC TATATGGTTT ACATGCCTTG TTTAAAGGAG ATTTCCGCAT ATCATCTCTC 
CGTGATGAGT GGATTTTTGC TGATATGGAG CTTTTAAGAA AAGTAGTAGT TCCTGGCATA 
CGTATGTCAC TAAAACTTCA TCAGGACCAC TTTACATCTC CAGATGAGTA TGATGATCCT 
GCAGTTTTGT ATGAAGCCAT TACATCACAT GAGCAGAACC TTGTTATTGC GCATGAGGGA 
GATCCAGCGT GGAGGAGTGC TGTTCTTTCT AACTCACCTT CTCTTCTCGC GCTGCGTCAT 
GTGATGGATG ATGGAACCAA CGAATACAAG ATCATAATGT TAAATAAACG TTATCTTAGT 
TTTAGAGTCA TTAAGGTAAA CAAGGAGTGT GTGCGGGGCT TATGGGCTGG TCAGCAGCAA 
GAATTGGTCT TTCTTCGTAA TCGGAACCCT GAAAGAGGCA GCATCCAAAA TGCAAAGCAG 
GCTCTCAGGA ATATGATCAA TTCATCATGT GACCAACCTA TTGGATACCC AATTTATGTT 
TCTCCACTGA CAACCTCTTA TTCAGACAGC CATGATCAGC TTAAACAGAT ATTTGGTGGG 
CCAATTAGCT TGGCCAATAT CCGGAACTTC ATTGTATCTA CATGGCACAG ATTAAGAAAA 
GGTTGCGGTG CAGGATGCAA TAGTGGTGGA AATATTGAGG ACTCTGACAC TGGAGGCGGG 
ACCTCCATTA ATAGCAACAA TCCACCAAAT GCAAATGAAA CTCAGAGCAG TCTGTCACAA 
GTTGGTGTCC CAGGTGGAAC AGGGCATGTG CCGAATGCAC GGCTCCAGCC TCCGGGGATG 
CCTCATCCAT CAACACTAGG AACCAGTCAC AGTTCTCACT CCATGCAGTC AAGCCTTGTC 
AGACATTCCC CAGCTCGTGG ATCAGTAGCA AGTCAATCAT CTTACTGCTA CAACAGCCGT 
CACTCATCAC TTAGAACATC CACAACAGGA TTTGTGCCTT GTAGGCGCTC CTCTACCAGC 
CAGATATCTC TTCGCAATCT CCCCTCATCC ATTCAGTCCC GTCTTTCCAT GGTGAACCAA 
ACAGACCCCA CAGGACAGTC AGTTTTGGTT GGCCTTCCTT CCTCCAGCAG CTCAAGCCAG 
AGCATCCCTG CATGCAAAAG ACACACCTTA GTGGGCTTTT TAGGAACAGA AGGAGGCAAT 
AACCTCACTG AAACGCAATC CTGTGGCAAT TCTAGTCCAG CAACCCATTC AAGAGCCAAA 
AAGGAAGAGG TTGTTTACAG AGTCCAGATT GTGGATCCAA GCCAAGTGCT TGAAGGCGTG 
AATATATCCA AAAGAAAGGA GCTGCAGTGG CCAGATGAGC TGGTGCGGCT AAAAGCCGGC 
AAGAACAGCT GGAAAGACTG GAGCCCTCAG GAAGGCATGG AAGGCCATGT AATTCACCGC 
TGGGTGCCTT GCAGCAGAGA CCCTTGTAAT AGGTCCCACA TTGACAAAAC AATCCTGCTG 
ATTAAGATTG AGGACAAATA TGTTGCTGTG ATTGAAACTG GTGTGCTTGA ACTCGGAGCT 
GAAGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pcnx1 ( XM_031890966.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746826.1 Xenopus tropicalis pecanex 1 (pcnx1), transcript variant X4, mRNA.

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CloneID	OXa64210
Clone ID Related Accession (Same CDS sequence)	XM_031890966.1
Accession Version	XM_031890966.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6954bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pecanex-like protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCC AGACACTGCA AATCCTCCGG CAGGGAGTCT GGGCTTCCAT CACCGGAGGC 
TGGTACTACG ACCCCCACCA GAGTACCTTT GTCAATACCC TACACCTCTA CATCTGGCTC 
TTCCTCCTCT GCTTCCCCTT CACCCTCTAC ATGGCACTAC CCACATCTTT GGTGATTGTT 
GCTGTGTACT GCCCAGTCGT TGCAGCTGTT TTTATTGTTT TGAAGCTTGT TAACTACCGT 
CTGCACAGGG CATTGGATGA AGGAGAAATT GTGGAGAGGA GTGCTAGTGA GTTGACTGAG 
AGGGTGGCAA AGGCAGAACA CAGTGACAGC TCTGCAAGAA GAGAGGACAG CAATGGACCT 
GGTGATCCAG GTGGAGGGAT TGAAATGTCT GAATTTGTTC GAGAAGCAAC TCCTCCAGTT 
GGATGCAGTT CACGTAATTC CTACTCTGGG TTGGACCCAA GCAATCAGGT TGGTGTGTCT 
CGTTATGGAG GTGCATCTGG AAGCAAAGGG GATACAGATG CTGCAAAGAC ATCAGATGAT 
ATCAGTCTGA GTCTTGGCCA GAGTTACAGT TTGTGCAAAG AAGCAAGTGA AGACCAAGAT 
TTGACTGTGG ATCAGAAGCT TTTCCGTCTC GTCTCCAATG ACTCCTTTGT CTCCATGCAG 
CCATCTATAT CCTCGGGTGG AAAAGACTTA ACTAGAGACT TCAGTGACAA AGGATCAATC 
CCAACACACA GTCATCATCA CCATGTGGAT CTGTCTCTGT CTAATGCTTG TGACACAGAA 
TTGGCCTCAC TTGTTCCATT GCATTCACAT TCGTACAGGA AAGATCATCG ACCAAGAGGT 
GTCCCAAGGA CTTCAAGTTC TGCTGTTGCT TTCCCAGACA CTTCTCTCAC TGACTTTTCA 
CTCTATCAAC CAAGGCGCGG GCTGGATCCT GTTAGTGAAC TTGAAGCTTC CAAACCACAT 
TCTGGGTCCA AAGATTCACT AGTAGATAAT GCTTGTGCTT CAGGGATTTT TCAATTAGAC 
TGTCTAAAAA GCAAAGGAGC CCAGCAGCTT TCAAGAAGTG GTAGTGTTAA ACACATGAAG 
ACTGACAAAA GCATGGATAG TTTAAGAAGC CTCAGCACGA GAAGCAGTGG ATCCACAGAA 
AGTTATTGTA GTGGAACAGA TCGGGACACA AATAGCACTG TTAGCAGCTA CAAAAGTGAA 
CAGACCAGTT CAACTCATGT TGAAAGTATT TTATCTGAGC ATGAGGATGT TACTGCTGCA 
AAGAGAGTCC ATTGTAGTGA CCTGGAGGAA GAACATAGCT CTGCTACTGA TAAAAGGACT 
AGTAGTATAA GAACTGCTTT AGAAAGAAAC ACTGCTCAGG AAGCCAAGGA TGCCAACACA 
TCTGATGATG TACAAAGTCC AAGGGGTCTT AGCTCATCAG CATCAGAGGA AGCTAATAAG 
AATCCACACG CAAATGAGTT AACTGTACAA ACAGACTGGC TTCCAGGTAA ACTGTCAGTG 
AACATTGATG AACCTGGGGA GAAACCAGTC ATTTTAGTTG ACTTGAGATC TTTGGAAGAA 
ACGGGTATTA AGCAGAGAGA CAATGACGTA CGACCCAAAT CATCTAGTTT AATACATCGA 
ACATCCTCTG CTCATAAATC ATGCAGGAGA CGGACAGGTA AAAAAAGAGC CAGCAGTTTT 
GATGCAAGTA GACACAAAGA ATATGTTTCC TTTCAAGGTA TAGCTGATAC CAAACCTCAC 
AGTACTGGCT TTTGTCATGA TGAAGATTCT AGCGACCAAA GCGACTTGAG CAGGACCTCA 
AGCGTTCAGT CGGCCCACCG TTTCAGCAGC GATAGCTCTT CCAGCACTAC TTCCCATTCA 
TGCCAGTCTC CTGAAGCAAG ATACAGTGCC CTAAGGACAA AATATCGAAT AAAAGAAAGA 
GGCACAGATT CACATCACAC ACACCTAAAT TGTGAAGGAA CTAGTAAAAA GCGGTCCAAC 
CGCAGGACTT CTAGTGCAAG TGGTACAAAA AGCCGAGCTA GAGTATTAAG TCTGGACAGT 
GGCACTGTAG TTAGTTTGAG TGATGCCAAC AGACTGGCCG CTCCTGAAAA TATAAAAACC 
TTAACCACTT CAAAATCAGA TCTAGAAGCC AAAGAAGGCG AAGTGCTAGA TCAGATGTCT 
CTGTTGGGGC GAGCTTCACA GTTAGAATCT GTAACACGGT CAAGAAATAG TTTGCCCAGC 
CAGGTTACTT TCCAAGATGT TGGAGAGCGA GACGCATCTG TTGGAGCACA CGCCAGTGAG 
GAGACAGTAA CATTTCGCCG CGAACGCAGC ACATTTAGGC GCCAAGCGGT TCGGCGCAGA 
CACAATGCAG GGAGTAATCC CACCCCTCCT GCGTCACTCA TCGGATCACC TCTGAGCCTT 
CAAGATGGTC ATCAAGGCCA GCAGTCATCA ACCCAGGTCA AAGTACAGTC CCGCCCCCCT 
TCCCAGGCTG CAGTGCTCAG CGCTAGCGCC TCCTTGCTGG TGAGAAATGG GAGTGTCCAC 
TTAGAAGCGT CTCATGACAA TGCATCTGCC GTAGGCAGTA GCAGTTTGCA CGATGAACTT 
GGTATGCAGG CCTTATGTAA CCTTGGTGAC ATGGAAGTTT TAATGCAGCA CATTTTGGGT 
AAATTTACCC CATCTCTATA TGAGACTGGA GGTTGTGACA TGTCACTTGT AAACTTTGAG 
CCAGCTGCAA GAAGGACGTC TAATAATCTT TGTGACACAG ATTCCCATGT ATCAAGTTCT 
ACCTCAGTTC GATTCTACCC ACATGATCTA CTCTCTCTTC CCCAGATCCG ATTAAACAGG 
CTGCTAACCA TAGATACAGA TCTGTTAGAG CAGCAGGATA TTGATCTAAG CCCTGACCTG 
CAGGACCAGA TGCAACCACA GGAACAGGCT GCCCAAAAAG TAAAACACTA CTACCGGTTT 
TGGATTCTCC CAAAGCTGTG GGTCGGCATC AACTTTGACA GGCTTACACT CTTAGCCTTG 
TTCGATAGGA ACCGCGAGGT CCTAGAAAAT GTGCTGTCTG TCATTCTCGC CATTCTAGTT 
GCCTTTTTGG GCTCTGTTCT TCTCATAGAA GGCTTCTTTA AAGATATCTG GGTCTTCCAG 
TTTTGTCTTG TTATAGCCAG CTGTCAATAC TCTCTCCTTA AGAGTGTTCA ACCAGATTCT 
TCTTCCCCAA GACATGGCCA TAATCGTATC ATTGCCTATA GTAGGCCCGT TTACTTCTGT 
GCCTGTTGCG GCCTTATTTG GTTATTGGAT TATGGAAGCA AAAACATGTC TACCACCAAA 
TACAAACTAT ATGGAATGGC CTTCACCAAT CCTCTGCTTC TAGTGTCAGC CAGGGACTTA 
GTCATTGTCT TCACACTCTG TTTCCCAGTC GTGTTCTTTA TTGGTCTCCT GCCTCAGGTG 
AACACATTCG TCATGTACCT GTGTGAGCAA GTAGATATAC ATGTGTTTGG TGGAAATGCT 
ACCACCAGCT TGCTTGCAGC AATTTACAGC TTTATCCGTA GCATTGTGGC CATAGCTTTA 
CTCTACGGGC TGTGTTACGG TGCTCTTAAG GATCCATGGG ATGCACAACA CATTCCTGTA 
TTATTCTCTA TTTTCTGTGG TGTTCTTGTG GCAGTTTCAT ACCATCTCAG CCGACAGAGC 
AGCGATCCCT CTGTCCTCAT CTCACTGGTG CAATCAAAGA TTTTTCCAAA CAGCGAGGAT 
ACAAATCCTG AAGACCCACT ATCAGAAGTG AAAGACCCTC TTCCAGAAAA ACTGAGAAGC 
TCTGTTAATG AACGACTTCA GTCAGACGTA GTAGTCTGTG TCGTTATTGC AGTGTTGTAC 
TTTGCAATTC ATGTCAGCAC CGTATTTACT GCATTACAGC CGGTTCTGAG CTACGTACTC 
TATGCACTAG TTGGTTCTGT TGGACTCCTT ACTCACTACA TTCTACCACA AGTACGGAAA 
CAGCTTCCTT GGCACTGCTT CTCCCACCCG CTGCTGAAAA CTAAGGAATA TTACCAATTT 
GAAGTTCGAA ATGCTGCTCA TCTGATGTGG TTTGAGAAGG CTCACATCTG GCTGCTTTTT 
ATCGAGAAGA ACATTATTTA TCCTCTTATA GTTCTCAATG AGCTTAGCAT TGGTGCTAAG 
GATATCGTCA GCCCGAAAAA GCTGGATGCA GAAGTTGGTG CATTGTTGAT CACCATTGCC 
GGAATGAAGC TGCTGAGGTC GTCATTTAGC AGTCCCACCT GCCAGTATGC CACTGTCATT 
TTCACTGTCC TCTTCTTTAC TTTTGACTAC AAGGAGTATA CAGAAAAACT GCTGCTAGAT 
CTGTTCTTTA TGTCAATCCT CTTTAGCAAG CTCTGGGAAC TGTTTTTCAA ACTGCGCTTT 
GTCTACACCT ATATTGCCCC TTGGCAGATC ACGTGGGGAT CAGCATTCCA TGCTTTCGCA 
CAGCCATTTG CTGTACCTCA TTCTGCAATG CTGTTTGTAC AGGCAATTGT TTCTGCACTG 
TTCTCCACTC CCCTGAACCC TTTCTTAGGG AGTGCAATCT TTATTACATC ATATGTACGC 
CCTGTCAAGT TTTGGGAGAG AGACTACAAT ACAAAGAGAG TTGACCACTC AAACACAAGA 
TTAGCCTCTC AGCTTGACAG AAATCCAGGT TCAGATGACA ACAACTTGAA TTCCATATTT 
TATGAGCATC TCACACGCTC CCTTCAGCAT AGTCTGTGTG GTGATCTGTT GCTTGGCCGC 
TGGGGAAATT ACAATACTGG GGATTGCTTT ATTCTGGCCT CAGATTATCT AAACGCCTTG 
GTGCACCTTA TCGAAATAGG CAATGGTCTG GTCACCTTCC AGCTAAGAGG ACTAGAGTTC 
AGAGGTACCT ACTGCCAGCA GCGTGAAGTT GAAGCAATCA CTGAGGGTGT AGAGGAAGAT 
GAAGGCTTTT GCTGTTGCGA ACCTGGACAT ATTCCTCATA TGCTGTCTTT CAATGCTGCC 
TTTGGGCAGC GCTGGCTGGC ATGGGAAGTC TTGGTTACAA AGTATGTTCT GGAAGGTTAC 
AGCATCACAG ACAACAGTGC AGCCTCTATG CTTCAAGTGT TTGACCTTCG CAGGATCCTC 
ACTACCTATT ATGTTAAGGG CATTATATAC TATGTGACAG CATCTGCAAA ACTGGAGGAG 
TGGTTAGCAA ATGAGACAAT GCAGGAAGGT CTTCGCCAGT GTTCTGATCA TTGTTATGTT 
GATGTAGATC CAACTTTCAA CCCAAATATT GATGAAGACT ATGACCTTCG CTTAGCAGGC 
ATTTCACGGG AGAGCTTCTG TGCAAGCTAT CTTAACTGGA TTAAGTACTG TTCCTCTAGA 
AGGGAGAAGC CTCTTGATGC TGAAAAGGAT TCACCTTTGG TAACACTTTG TTATGGGCTC 
TGTATTTTGG GGAGAAGGGC ACTTGGTACA GCTTCTCATC ACATGTCCAG CAATTTGGAG 
TCCTTTCTAT ATGGTTTACA TGCCTTGTTT AAAGGAGATT TCCGCATATC ATCTCTCCGT 
GATGAGTGGA TTTTTGCTGA TATGGAGCTT TTAAGAAAAG TAGTAGTTCC TGGCATACGT 
ATGTCACTAA AACTTCATCA GGACCACTTT ACATCTCCAG ATGAGTATGA TGATCCTGCA 
GTTTTGTATG AAGCCATTAC ATCACATGAG CAGAACCTTG TTATTGCGCA TGAGGGAGAT 
CCAGCGTGGA GGAGTGCTGT TCTTTCTAAC TCACCTTCTC TTCTCGCGCT GCGTCATGTG 
ATGGATGATG GAACCAACGA ATACAAGATC ATAATGTTAA ATAAACGTTA TCTTAGTTTT 
AGAGTCATTA AGGTAAACAA GGAGTGTGTG CGGGGCTTAT GGGCTGGTCA GCAGCAAGAA 
TTGGTCTTTC TTCGTAATCG GAACCCTGAA AGAGGCAGCA TCCAAAATGC AAAGCAGGCT 
CTCAGGAATA TGATCAATTC ATCATGTGAC CAACCTATTG GATACCCAAT TTATGTTTCT 
CCACTGACAA CCTCTTATTC AGACAGCCAT GATCAGCTTA AACAGATATT TGGTGGGCCA 
ATTAGCTTGG CCAATATCCG GAACTTCATT GTATCTACAT GGCACAGATT AAGAAAAGGT 
TGCGGTGCAG GATGCAATAG TGGTGGAAAT ATTGAGGACT CTGACACTGG AGGCGGGACC 
TCCATTAATA GCAACAATCC ACCAAATGCA AATGAAACTC AGAGCAGTCT GTCACAAGTT 
GGTGTCCCAG GTGGAACAGG GCATGTGCCG AATGCACGGC TCCAGCCTCC GGGGATGCCT 
CATCCATCAA CACTAGGAAC CAGTCACAGT TCTCACTCCA TGCAGTCAAG CCTTGTCAGA 
CATTCCCCAG CTCGTGGATC AGTAGCAAGT CAATCATCTT ACTGCTACAA CAGCCGTCAC 
TCATCACTTA GAACATCCAC AACAGGATTT GTGCCTTGTA GGCGCTCCTC TACCAGCCAG 
ATATCTCTTC GCAATCTCCC CTCATCCATT CAGTCCCGTC TTTCCATGGT GAACCAAACA 
GACCCCACAG GACAGTCAGT TTTGGTTGGC CTTCCTTCCT CCAGCAGCTC AAGCCAGAGC 
ATCCCTGCAT GCAAAAGACA CACCTTAGTG GGCTTTTTAG GAACAGAAGG AGGCAATAAC 
CTCACTGAAA CGCAATCCTG TGGCAATTCT AGTCCAGCAA CCCATTCAAG AGCCAAAAAG 
GAAGAGGTTG TTTACAGAGT CCAGATTGTG GATCCAAGCC AAGTGCTTGA AGGCGTGAAT 
ATATCCAAAA GAAAGGAGCT GCAGTGGCCA GATGAGCTGG TGCGGCTAAA AGCCGGCAAG 
AACAGCTGGA AAGACTGGAG CCCTCAGGAA GGCATGGAAG GCCATGTAGG TTTGGCTGGT 
TACCATAAAT ATCTTCATGG GAACAAATAT TTTCCTGTTA GTCCTCAGCA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746826.1
CDS	277..7230
Translation	MGSQTLQILRQGVWASITGGWYYDPHQSTFVNTLHLYIWLFLLCFPFTLYMALPTSLVIVAVYCPVVAAVFIVLKLVNYRLHRALDEGEIVERSASELTERVAKAEHSDSSARREDSNGPGDPGGGIEMSEFVREATPPVGCSSRNSYSGLDPSNQVGVSRYGGASGSKGDTDAAKTSDDISLSLGQSYSLCKEASEDQDLTVDQKLFRLVSNDSFVSMQPSISSGGKDLTRDFSDKGSIPTHSHHHHVDLSLSNACDTELASLVPLHSHSYRKDHRPRGVPRTSSSAVAFPDTSLTDFSLYQPRRGLDPVSELEASKPHSGSKDSLVDNACASGIFQLDCLKSKGAQQLSRSGSVKHMKTDKSMDSLRSLSTRSSGSTESYCSGTDRDTNSTVSSYKSEQTSSTHVESILSEHEDVTAAKRVHCSDLEEEHSSATDKRTSSIRTALERNTAQEAKDANTSDDVQSPRGLSSSASEEANKNPHANELTVQTDWLPGKLSVNIDEPGEKPVILVDLRSLEETGIKQRDNDVRPKSSSLIHRTSSAHKSCRRRTGKKRASSFDASRHKEYVSFQGIADTKPHSTGFCHDEDSSDQSDLSRTSSVQSAHRFSSDSSSSTTSHSCQSPEARYSALRTKYRIKERGTDSHHTHLNCEGTSKKRSNRRTSSASGTKSRARVLSLDSGTVVSLSDANRLAAPENIKTLTTSKSDLEAKEGEVLDQMSLLGRASQLESVTRSRNSLPSQVTFQDVGERDASVGAHASEETVTFRRERSTFRRQAVRRRHNAGSNPTPPASLIGSPLSLQDGHQGQQSSTQVKVQSRPPSQAAVLSASASLLVRNGSVHLEASHDNASAVGSSSLHDELGMQALCNLGDMEVLMQHILGKFTPSLYETGGCDMSLVNFEPAARRTSNNLCDTDSHVSSSTSVRFYPHDLLSLPQIRLNRLLTIDTDLLEQQDIDLSPDLQDQMQPQEQAAQKVKHYYRFWILPKLWVGINFDRLTLLALFDRNREVLENVLSVILAILVAFLGSVLLIEGFFKDIWVFQFCLVIASCQYSLLKSVQPDSSSPRHGHNRIIAYSRPVYFCACCGLIWLLDYGSKNMSTTKYKLYGMAFTNPLLLVSARDLVIVFTLCFPVVFFIGLLPQVNTFVMYLCEQVDIHVFGGNATTSLLAAIYSFIRSIVAIALLYGLCYGALKDPWDAQHIPVLFSIFCGVLVAVSYHLSRQSSDPSVLISLVQSKIFPNSEDTNPEDPLSEVKDPLPEKLRSSVNERLQSDVVVCVVIAVLYFAIHVSTVFTALQPVLSYVLYALVGSVGLLTHYILPQVRKQLPWHCFSHPLLKTKEYYQFEVRNAAHLMWFEKAHIWLLFIEKNIIYPLIVLNELSIGAKDIVSPKKLDAEVGALLITIAGMKLLRSSFSSPTCQYATVIFTVLFFTFDYKEYTEKLLLDLFFMSILFSKLWELFFKLRFVYTYIAPWQITWGSAFHAFAQPFAVPHSAMLFVQAIVSALFSTPLNPFLGSAIFITSYVRPVKFWERDYNTKRVDHSNTRLASQLDRNPGSDDNNLNSIFYEHLTRSLQHSLCGDLLLGRWGNYNTGDCFILASDYLNALVHLIEIGNGLVTFQLRGLEFRGTYCQQREVEAITEGVEEDEGFCCCEPGHIPHMLSFNAAFGQRWLAWEVLVTKYVLEGYSITDNSAASMLQVFDLRRILTTYYVKGIIYYVTASAKLEEWLANETMQEGLRQCSDHCYVDVDPTFNPNIDEDYDLRLAGISRESFCASYLNWIKYCSSRREKPLDAEKDSPLVTLCYGLCILGRRALGTASHHMSSNLESFLYGLHALFKGDFRISSLRDEWIFADMELLRKVVVPGIRMSLKLHQDHFTSPDEYDDPAVLYEAITSHEQNLVIAHEGDPAWRSAVLSNSPSLLALRHVMDDGTNEYKIIMLNKRYLSFRVIKVNKECVRGLWAGQQQELVFLRNRNPERGSIQNAKQALRNMINSSCDQPIGYPIYVSPLTTSYSDSHDQLKQIFGGPISLANIRNFIVSTWHRLRKGCGAGCNSGGNIEDSDTGGGTSINSNNPPNANETQSSLSQVGVPGGTGHVPNARLQPPGMPHPSTLGTSHSSHSMQSSLVRHSPARGSVASQSSYCYNSRHSSLRTSTTGFVPCRRSSTSQISLRNLPSSIQSRLSMVNQTDPTGQSVLVGLPSSSSSSQSIPACKRHTLVGFLGTEGGNNLTETQSCGNSSPATHSRAKKEEVVYRVQIVDPSQVLEGVNISKRKELQWPDELVRLKAGKNSWKDWSPQEGMEGHVGLAGYHKYLHGNKYFPVSPQQ

Target ORF information:

RefSeq Version	XM_031890966.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pecanex 1 (pcnx1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031890966.1

ORF Insert Sequence:

ATGGGTTCCC AGACACTGCA AATCCTCCGG CAGGGAGTCT GGGCTTCCAT CACCGGAGGC 
TGGTACTACG ACCCCCACCA GAGTACCTTT GTCAATACCC TACACCTCTA CATCTGGCTC 
TTCCTCCTCT GCTTCCCCTT CACCCTCTAC ATGGCACTAC CCACATCTTT GGTGATTGTT 
GCTGTGTACT GCCCAGTCGT TGCAGCTGTT TTTATTGTTT TGAAGCTTGT TAACTACCGT 
CTGCACAGGG CATTGGATGA AGGAGAAATT GTGGAGAGGA GTGCTAGTGA GTTGACTGAG 
AGGGTGGCAA AGGCAGAACA CAGTGACAGC TCTGCAAGAA GAGAGGACAG CAATGGACCT 
GGTGATCCAG GTGGAGGGAT TGAAATGTCT GAATTTGTTC GAGAAGCAAC TCCTCCAGTT 
GGATGCAGTT CACGTAATTC CTACTCTGGG TTGGACCCAA GCAATCAGGT TGGTGTGTCT 
CGTTATGGAG GTGCATCTGG AAGCAAAGGG GATACAGATG CTGCAAAGAC ATCAGATGAT 
ATCAGTCTGA GTCTTGGCCA GAGTTACAGT TTGTGCAAAG AAGCAAGTGA AGACCAAGAT 
TTGACTGTGG ATCAGAAGCT TTTCCGTCTC GTCTCCAATG ACTCCTTTGT CTCCATGCAG 
CCATCTATAT CCTCGGGTGG AAAAGACTTA ACTAGAGACT TCAGTGACAA AGGATCAATC 
CCAACACACA GTCATCATCA CCATGTGGAT CTGTCTCTGT CTAATGCTTG TGACACAGAA 
TTGGCCTCAC TTGTTCCATT GCATTCACAT TCGTACAGGA AAGATCATCG ACCAAGAGGT 
GTCCCAAGGA CTTCAAGTTC TGCTGTTGCT TTCCCAGACA CTTCTCTCAC TGACTTTTCA 
CTCTATCAAC CAAGGCGCGG GCTGGATCCT GTTAGTGAAC TTGAAGCTTC CAAACCACAT 
TCTGGGTCCA AAGATTCACT AGTAGATAAT GCTTGTGCTT CAGGGATTTT TCAATTAGAC 
TGTCTAAAAA GCAAAGGAGC CCAGCAGCTT TCAAGAAGTG GTAGTGTTAA ACACATGAAG 
ACTGACAAAA GCATGGATAG TTTAAGAAGC CTCAGCACGA GAAGCAGTGG ATCCACAGAA 
AGTTATTGTA GTGGAACAGA TCGGGACACA AATAGCACTG TTAGCAGCTA CAAAAGTGAA 
CAGACCAGTT CAACTCATGT TGAAAGTATT TTATCTGAGC ATGAGGATGT TACTGCTGCA 
AAGAGAGTCC ATTGTAGTGA CCTGGAGGAA GAACATAGCT CTGCTACTGA TAAAAGGACT 
AGTAGTATAA GAACTGCTTT AGAAAGAAAC ACTGCTCAGG AAGCCAAGGA TGCCAACACA 
TCTGATGATG TACAAAGTCC AAGGGGTCTT AGCTCATCAG CATCAGAGGA AGCTAATAAG 
AATCCACACG CAAATGAGTT AACTGTACAA ACAGACTGGC TTCCAGGTAA ACTGTCAGTG 
AACATTGATG AACCTGGGGA GAAACCAGTC ATTTTAGTTG ACTTGAGATC TTTGGAAGAA 
ACGGGTATTA AGCAGAGAGA CAATGACGTA CGACCCAAAT CATCTAGTTT AATACATCGA 
ACATCCTCTG CTCATAAATC ATGCAGGAGA CGGACAGGTA AAAAAAGAGC CAGCAGTTTT 
GATGCAAGTA GACACAAAGA ATATGTTTCC TTTCAAGGTA TAGCTGATAC CAAACCTCAC 
AGTACTGGCT TTTGTCATGA TGAAGATTCT AGCGACCAAA GCGACTTGAG CAGGACCTCA 
AGCGTTCAGT CGGCCCACCG TTTCAGCAGC GATAGCTCTT CCAGCACTAC TTCCCATTCA 
TGCCAGTCTC CTGAAGCAAG ATACAGTGCC CTAAGGACAA AATATCGAAT AAAAGAAAGA 
GGCACAGATT CACATCACAC ACACCTAAAT TGTGAAGGAA CTAGTAAAAA GCGGTCCAAC 
CGCAGGACTT CTAGTGCAAG TGGTACAAAA AGCCGAGCTA GAGTATTAAG TCTGGACAGT 
GGCACTGTAG TTAGTTTGAG TGATGCCAAC AGACTGGCCG CTCCTGAAAA TATAAAAACC 
TTAACCACTT CAAAATCAGA TCTAGAAGCC AAAGAAGGCG AAGTGCTAGA TCAGATGTCT 
CTGTTGGGGC GAGCTTCACA GTTAGAATCT GTAACACGGT CAAGAAATAG TTTGCCCAGC 
CAGGTTACTT TCCAAGATGT TGGAGAGCGA GACGCATCTG TTGGAGCACA CGCCAGTGAG 
GAGACAGTAA CATTTCGCCG CGAACGCAGC ACATTTAGGC GCCAAGCGGT TCGGCGCAGA 
CACAATGCAG GGAGTAATCC CACCCCTCCT GCGTCACTCA TCGGATCACC TCTGAGCCTT 
CAAGATGGTC ATCAAGGCCA GCAGTCATCA ACCCAGGTCA AAGTACAGTC CCGCCCCCCT 
TCCCAGGCTG CAGTGCTCAG CGCTAGCGCC TCCTTGCTGG TGAGAAATGG GAGTGTCCAC 
TTAGAAGCGT CTCATGACAA TGCATCTGCC GTAGGCAGTA GCAGTTTGCA CGATGAACTT 
GGTATGCAGG CCTTATGTAA CCTTGGTGAC ATGGAAGTTT TAATGCAGCA CATTTTGGGT 
AAATTTACCC CATCTCTATA TGAGACTGGA GGTTGTGACA TGTCACTTGT AAACTTTGAG 
CCAGCTGCAA GAAGGACGTC TAATAATCTT TGTGACACAG ATTCCCATGT ATCAAGTTCT 
ACCTCAGTTC GATTCTACCC ACATGATCTA CTCTCTCTTC CCCAGATCCG ATTAAACAGG 
CTGCTAACCA TAGATACAGA TCTGTTAGAG CAGCAGGATA TTGATCTAAG CCCTGACCTG 
CAGGACCAGA TGCAACCACA GGAACAGGCT GCCCAAAAAG TAAAACACTA CTACCGGTTT 
TGGATTCTCC CAAAGCTGTG GGTCGGCATC AACTTTGACA GGCTTACACT CTTAGCCTTG 
TTCGATAGGA ACCGCGAGGT CCTAGAAAAT GTGCTGTCTG TCATTCTCGC CATTCTAGTT 
GCCTTTTTGG GCTCTGTTCT TCTCATAGAA GGCTTCTTTA AAGATATCTG GGTCTTCCAG 
TTTTGTCTTG TTATAGCCAG CTGTCAATAC TCTCTCCTTA AGAGTGTTCA ACCAGATTCT 
TCTTCCCCAA GACATGGCCA TAATCGTATC ATTGCCTATA GTAGGCCCGT TTACTTCTGT 
GCCTGTTGCG GCCTTATTTG GTTATTGGAT TATGGAAGCA AAAACATGTC TACCACCAAA 
TACAAACTAT ATGGAATGGC CTTCACCAAT CCTCTGCTTC TAGTGTCAGC CAGGGACTTA 
GTCATTGTCT TCACACTCTG TTTCCCAGTC GTGTTCTTTA TTGGTCTCCT GCCTCAGGTG 
AACACATTCG TCATGTACCT GTGTGAGCAA GTAGATATAC ATGTGTTTGG TGGAAATGCT 
ACCACCAGCT TGCTTGCAGC AATTTACAGC TTTATCCGTA GCATTGTGGC CATAGCTTTA 
CTCTACGGGC TGTGTTACGG TGCTCTTAAG GATCCATGGG ATGCACAACA CATTCCTGTA 
TTATTCTCTA TTTTCTGTGG TGTTCTTGTG GCAGTTTCAT ACCATCTCAG CCGACAGAGC 
AGCGATCCCT CTGTCCTCAT CTCACTGGTG CAATCAAAGA TTTTTCCAAA CAGCGAGGAT 
ACAAATCCTG AAGACCCACT ATCAGAAGTG AAAGACCCTC TTCCAGAAAA ACTGAGAAGC 
TCTGTTAATG AACGACTTCA GTCAGACGTA GTAGTCTGTG TCGTTATTGC AGTGTTGTAC 
TTTGCAATTC ATGTCAGCAC CGTATTTACT GCATTACAGC CGGTTCTGAG CTACGTACTC 
TATGCACTAG TTGGTTCTGT TGGACTCCTT ACTCACTACA TTCTACCACA AGTACGGAAA 
CAGCTTCCTT GGCACTGCTT CTCCCACCCG CTGCTGAAAA CTAAGGAATA TTACCAATTT 
GAAGTTCGAA ATGCTGCTCA TCTGATGTGG TTTGAGAAGG CTCACATCTG GCTGCTTTTT 
ATCGAGAAGA ACATTATTTA TCCTCTTATA GTTCTCAATG AGCTTAGCAT TGGTGCTAAG 
GATATCGTCA GCCCGAAAAA GCTGGATGCA GAAGTTGGTG CATTGTTGAT CACCATTGCC 
GGAATGAAGC TGCTGAGGTC GTCATTTAGC AGTCCCACCT GCCAGTATGC CACTGTCATT 
TTCACTGTCC TCTTCTTTAC TTTTGACTAC AAGGAGTATA CAGAAAAACT GCTGCTAGAT 
CTGTTCTTTA TGTCAATCCT CTTTAGCAAG CTCTGGGAAC TGTTTTTCAA ACTGCGCTTT 
GTCTACACCT ATATTGCCCC TTGGCAGATC ACGTGGGGAT CAGCATTCCA TGCTTTCGCA 
CAGCCATTTG CTGTACCTCA TTCTGCAATG CTGTTTGTAC AGGCAATTGT TTCTGCACTG 
TTCTCCACTC CCCTGAACCC TTTCTTAGGG AGTGCAATCT TTATTACATC ATATGTACGC 
CCTGTCAAGT TTTGGGAGAG AGACTACAAT ACAAAGAGAG TTGACCACTC AAACACAAGA 
TTAGCCTCTC AGCTTGACAG AAATCCAGGT TCAGATGACA ACAACTTGAA TTCCATATTT 
TATGAGCATC TCACACGCTC CCTTCAGCAT AGTCTGTGTG GTGATCTGTT GCTTGGCCGC 
TGGGGAAATT ACAATACTGG GGATTGCTTT ATTCTGGCCT CAGATTATCT AAACGCCTTG 
GTGCACCTTA TCGAAATAGG CAATGGTCTG GTCACCTTCC AGCTAAGAGG ACTAGAGTTC 
AGAGGTACCT ACTGCCAGCA GCGTGAAGTT GAAGCAATCA CTGAGGGTGT AGAGGAAGAT 
GAAGGCTTTT GCTGTTGCGA ACCTGGACAT ATTCCTCATA TGCTGTCTTT CAATGCTGCC 
TTTGGGCAGC GCTGGCTGGC ATGGGAAGTC TTGGTTACAA AGTATGTTCT GGAAGGTTAC 
AGCATCACAG ACAACAGTGC AGCCTCTATG CTTCAAGTGT TTGACCTTCG CAGGATCCTC 
ACTACCTATT ATGTTAAGGG CATTATATAC TATGTGACAG CATCTGCAAA ACTGGAGGAG 
TGGTTAGCAA ATGAGACAAT GCAGGAAGGT CTTCGCCAGT GTTCTGATCA TTGTTATGTT 
GATGTAGATC CAACTTTCAA CCCAAATATT GATGAAGACT ATGACCTTCG CTTAGCAGGC 
ATTTCACGGG AGAGCTTCTG TGCAAGCTAT CTTAACTGGA TTAAGTACTG TTCCTCTAGA 
AGGGAGAAGC CTCTTGATGC TGAAAAGGAT TCACCTTTGG TAACACTTTG TTATGGGCTC 
TGTATTTTGG GGAGAAGGGC ACTTGGTACA GCTTCTCATC ACATGTCCAG CAATTTGGAG 
TCCTTTCTAT ATGGTTTACA TGCCTTGTTT AAAGGAGATT TCCGCATATC ATCTCTCCGT 
GATGAGTGGA TTTTTGCTGA TATGGAGCTT TTAAGAAAAG TAGTAGTTCC TGGCATACGT 
ATGTCACTAA AACTTCATCA GGACCACTTT ACATCTCCAG ATGAGTATGA TGATCCTGCA 
GTTTTGTATG AAGCCATTAC ATCACATGAG CAGAACCTTG TTATTGCGCA TGAGGGAGAT 
CCAGCGTGGA GGAGTGCTGT TCTTTCTAAC TCACCTTCTC TTCTCGCGCT GCGTCATGTG 
ATGGATGATG GAACCAACGA ATACAAGATC ATAATGTTAA ATAAACGTTA TCTTAGTTTT 
AGAGTCATTA AGGTAAACAA GGAGTGTGTG CGGGGCTTAT GGGCTGGTCA GCAGCAAGAA 
TTGGTCTTTC TTCGTAATCG GAACCCTGAA AGAGGCAGCA TCCAAAATGC AAAGCAGGCT 
CTCAGGAATA TGATCAATTC ATCATGTGAC CAACCTATTG GATACCCAAT TTATGTTTCT 
CCACTGACAA CCTCTTATTC AGACAGCCAT GATCAGCTTA AACAGATATT TGGTGGGCCA 
ATTAGCTTGG CCAATATCCG GAACTTCATT GTATCTACAT GGCACAGATT AAGAAAAGGT 
TGCGGTGCAG GATGCAATAG TGGTGGAAAT ATTGAGGACT CTGACACTGG AGGCGGGACC 
TCCATTAATA GCAACAATCC ACCAAATGCA AATGAAACTC AGAGCAGTCT GTCACAAGTT 
GGTGTCCCAG GTGGAACAGG GCATGTGCCG AATGCACGGC TCCAGCCTCC GGGGATGCCT 
CATCCATCAA CACTAGGAAC CAGTCACAGT TCTCACTCCA TGCAGTCAAG CCTTGTCAGA 
CATTCCCCAG CTCGTGGATC AGTAGCAAGT CAATCATCTT ACTGCTACAA CAGCCGTCAC 
TCATCACTTA GAACATCCAC AACAGGATTT GTGCCTTGTA GGCGCTCCTC TACCAGCCAG 
ATATCTCTTC GCAATCTCCC CTCATCCATT CAGTCCCGTC TTTCCATGGT GAACCAAACA 
GACCCCACAG GACAGTCAGT TTTGGTTGGC CTTCCTTCCT CCAGCAGCTC AAGCCAGAGC 
ATCCCTGCAT GCAAAAGACA CACCTTAGTG GGCTTTTTAG GAACAGAAGG AGGCAATAAC 
CTCACTGAAA CGCAATCCTG TGGCAATTCT AGTCCAGCAA CCCATTCAAG AGCCAAAAAG 
GAAGAGGTTG TTTACAGAGT CCAGATTGTG GATCCAAGCC AAGTGCTTGA AGGCGTGAAT 
ATATCCAAAA GAAAGGAGCT GCAGTGGCCA GATGAGCTGG TGCGGCTAAA AGCCGGCAAG 
AACAGCTGGA AAGACTGGAG CCCTCAGGAA GGCATGGAAG GCCATGTAGG TTTGGCTGGT 
TACCATAAAT ATCTTCATGG GAACAAATAT TTTCCTGTTA GTCCTCAGCA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pcnx1 ( XM_004917255.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004917312.2 Xenopus tropicalis pecanex 1 (pcnx1), transcript variant X5, mRNA.

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CloneID	OXa64211
Clone ID Related Accession (Same CDS sequence)	XM_004917255.4
Accession Version	XM_004917255.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6936bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pecanex-like protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004917255.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCC AGACACTGCA AATCCTCCGG CAGGGAGTCT GGGCTTCCAT CACCGGAGGC 
TGGTACTACG ACCCCCACCA GAGTACCTTT GTCAATACCC TACACCTCTA CATCTGGCTC 
TTCCTCCTCT GCTTCCCCTT CACCCTCTAC ATGGCACTAC CCACATCTTT GGTGATTGTT 
GCTGTGTACT GCCCAGTCGT TGCAGCTGTT TTTATTGTTT TGAAGCTTGT TAACTACCGT 
CTGCACAGGG CATTGGATGA AGGAGAAATT GTGGAGAGGA GTGCTAGTGA GTTGACTGAG 
AGGGTGGCAA AGGCAGAACA CAGTGACAGC TCTGCAAGAA GAGAGGACAG CAATGGACCT 
GGTGATCCAG GTGGAGGGAT TGAAATGTCT GAATTTGTTC GAGAAGCAAC TCCTCCAGTT 
GGATGCAGTT CACGTAATTC CTACTCTGGG TTGGACCCAA GCAATCAGGT TGGTGTGTCT 
CGTTATGGAG GTGCATCTGG AAGCAAAGAT TTGACTGTGG ATCAGAAGCT TTTCCGTCTC 
GTCTCCAATG ACTCCTTTGT CTCCATGCAG CCATCTATAT CCTCGGGTGG AAAAGACTTA 
ACTAGAGACT TCAGTGACAA AGGATCAATC CCAACACACA GTCATCATCA CCATGTGGAT 
CTGTCTCTGT CTAATGCTTG TGACACAGAA TTGGCCTCAC TTGTTCCATT GCATTCACAT 
TCGTACAGGA AAGATCATCG ACCAAGAGGT GTCCCAAGGA CTTCAAGTTC TGCTGTTGCT 
TTCCCAGACA CTTCTCTCAC TGACTTTTCA CTCTATCAAC CAAGGCGCGG GCTGGATCCT 
GTTAGTGAAC TTGAAGCTTC CAAACCACAT TCTGGGTCCA AAGATTCACT AGTAGATAAT 
GCTTGTGCTT CAGGGATTTT TCAATTAGAC TGTCTAAAAA GCAAAGGAGC CCAGCAGCTT 
TCAAGAAGTG GTAGTGTTAA ACACATGAAG ACTGACAAAA GCATGGATAG TTTAAGAAGC 
CTCAGCACGA GAAGCAGTGG ATCCACAGAA AGTTATTGTA GTGGAACAGA TCGGGACACA 
AATAGCACTG TTAGCAGCTA CAAAAGTGAA CAGACCAGTT CAACTCATGT TGAAAGTATT 
TTATCTGAGC ATGAGGATGT TACTGCTGCA AAGAGAGTCC ATTGTAGTGA CCTGGAGGAA 
GAACATAGCT CTGCTACTGA TAAAAGGACT AGTAGTATAA GAACTGCTTT AGAAAGAAAC 
ACTGCTCAGG AAGCCAAGGA TGCCAACACA TCTGATGATG TACAAAGTCC AAGGGGTCTT 
AGCTCATCAG CATCAGAGGA AGCTAATAAG AATCCACACG CAAATGAGTT AACTGTACAA 
ACAGACTGGC TTCCAGGTAA ACTGTCAGTG AACATTGATG AACCTGGGGA GAAACCAGTC 
ATTTTAGTTG ACTTGAGATC TTTGGAAGAA ACGGGTATTA AGCAGAGAGA CAATGACGTA 
CGACCCAAAT CATCTAGTTT AATACATCGA ACATCCTCTG CTCATAAATC ATGCAGGAGA 
CGGACAGGTA AAAAAAGAGC CAGCAGTTTT GATGCAAGTA GACACAAAGA ATATGTTTCC 
TTTCAAGGTA TAGCTGATAC CAAACCTCAC AGTACTGGCT TTTGTCATGA TGAAGATTCT 
AGCGACCAAA GCGACTTGAG CAGGACCTCA AGCGTTCAGT CGGCCCACCG TTTCAGCAGC 
GATAGCTCTT CCAGCACTAC TTCCCATTCA TGCCAGTCTC CTGAAGCAAG ATACAGTGCC 
CTAAGGACAA AATATCGAAT AAAAGAAAGA GGCACAGATT CACATCACAC ACACCTAAAT 
TGTGAAGGAA CTAGTAAAAA GCGGTCCAAC CGCAGGACTT CTAGTGCAAG TGGTACAAAA 
AGCCGAGCTA GAGTATTAAG TCTGGACAGT GGCACTGTAG TTAGTTTGAG TGATGCCAAC 
AGACTGGCCG CTCCTGAAAA TATAAAAACC TTAACCACTT CAAAATCAGA TCTAGAAGCC 
AAAGAAGGCG AAGTGCTAGA TCAGATGTCT CTGTTGGGGC GAGCTTCACA GTTAGAATCT 
GTAACACGGT CAAGAAATAG TTTGCCCAGC CAGGTTACTT TCCAAGATGT TGGAGAGCGA 
GACGCATCTG TTGGAGCACA CGCCAGTGAG GAGACAGTAA CATTTCGCCG CGAACGCAGC 
ACATTTAGGC GCCAAGCGGT TCGGCGCAGA CACAATGCAG GGAGTAATCC CACCCCTCCT 
GCGTCACTCA TCGGATCACC TCTGAGCCTT CAAGATGGTC ATCAAGGCCA GCAGTCATCA 
ACCCAGGTCA AAGTACAGTC CCGCCCCCCT TCCCAGGCTG CAGTGCTCAG CGCTAGCGCC 
TCCTTGCTGG TGAGAAATGG GAGTGTCCAC TTAGAAGCGT CTCATGACAA TGCATCTGCC 
GTAGGCAGTA GCAGTTTGCA CGATGAACTT GGTATGCAGG CCTTATGTAA CCTTGGTGAC 
ATGGAAGTTT TAATGCAGCA CATTTTGGGT AAATTTACCC CATCTCTATA TGAGACTGGA 
GGTTGTGACA TGTCACTTGT AAACTTTGAG CCAGCTGCAA GAAGGACGTC TAATAATCTT 
TGTGACACAG ATTCCCATGT ATCAAGTTCT ACCTCAGTTC GATTCTACCC ACATGATCTA 
CTCTCTCTTC CCCAGATCCG ATTAAACAGG CTGCTAACCA TAGATACAGA TCTGTTAGAG 
CAGCAGGATA TTGATCTAAG CCCTGACCTG CAGGACCAGA TGCAACCACA GGAACAGGCT 
GCCCAAAAAG TAAAACACTA CTACCGGTTT TGGATTCTCC CAAAGCTGTG GGTCGGCATC 
AACTTTGACA GGCTTACACT CTTAGCCTTG TTCGATAGGA ACCGCGAGGT CCTAGAAAAT 
GTGCTGTCTG TCATTCTCGC CATTCTAGTT GCCTTTTTGG GCTCTGTTCT TCTCATAGAA 
GGCTTCTTTA AAGATATCTG GGTCTTCCAG TTTTGTCTTG TTATAGCCAG CTGTCAATAC 
TCTCTCCTTA AGAGTGTTCA ACCAGATTCT TCTTCCCCAA GACATGGCCA TAATCGTATC 
ATTGCCTATA GTAGGCCCGT TTACTTCTGT GCCTGTTGCG GCCTTATTTG GTTATTGGAT 
TATGGAAGCA AAAACATGTC TACCACCAAA TACAAACTAT ATGGAATGGC CTTCACCAAT 
CCTCTGCTTC TAGTGTCAGC CAGGGACTTA GTCATTGTCT TCACACTCTG TTTCCCAGTC 
GTGTTCTTTA TTGGTCTCCT GCCTCAGGTG AACACATTCG TCATGTACCT GTGTGAGCAA 
GTAGATATAC ATGTGTTTGG TGGAAATGCT ACCACCAGCT TGCTTGCAGC AATTTACAGC 
TTTATCCGTA GCATTGTGGC CATAGCTTTA CTCTACGGGC TGTGTTACGG TGCTCTTAAG 
GATCCATGGG ATGCACAACA CATTCCTGTA TTATTCTCTA TTTTCTGTGG TGTTCTTGTG 
GCAGTTTCAT ACCATCTCAG CCGACAGAGC AGCGATCCCT CTGTCCTCAT CTCACTGGTG 
CAATCAAAGA TTTTTCCAAA CAGCGAGGAT ACAAATCCTG AAGACCCACT ATCAGAAGTG 
AAAGACCCTC TTCCAGAAAA ACTGAGAAGC TCTGTTAATG AACGACTTCA GTCAGACGTA 
GTAGTCTGTG TCGTTATTGC AGTGTTGTAC TTTGCAATTC ATGTCAGCAC CGTATTTACT 
GCATTACAGC CGGTTCTGAG CTACGTACTC TATGCACTAG TTGGTTCTGT TGGACTCCTT 
ACTCACTACA TTCTACCACA AGTACGGAAA CAGCTTCCTT GGCACTGCTT CTCCCACCCG 
CTGCTGAAAA CTAAGGAATA TTACCAATTT GAAGTTCGAA ATGCTGCTCA TCTGATGTGG 
TTTGAGAAGG CTCACATCTG GCTGCTTTTT ATCGAGAAGA ACATTATTTA TCCTCTTATA 
GTTCTCAATG AGCTTAGCAT TGGTGCTAAG GATATCGTCA GCCCGAAAAA GCTGGATGCA 
GAAGTTGGTG CATTGTTGAT CACCATTGCC GGAATGAAGC TGCTGAGGTC GTCATTTAGC 
AGTCCCACCT GCCAGTATGC CACTGTCATT TTCACTGTCC TCTTCTTTAC TTTTGACTAC 
AAGGAGTATA CAGAAAAACT GCTGCTAGAT CTGTTCTTTA TGTCAATCCT CTTTAGCAAG 
CTCTGGGAAC TGTTTTTCAA ACTGCGCTTT GTCTACACCT ATATTGCCCC TTGGCAGATC 
ACGTGGGGAT CAGCATTCCA TGCTTTCGCA CAGCCATTTG CTGTACCTCA TTCTGCAATG 
CTGTTTGTAC AGGCAATTGT TTCTGCACTG TTCTCCACTC CCCTGAACCC TTTCTTAGGG 
AGTGCAATCT TTATTACATC ATATGTACGC CCTGTCAAGT TTTGGGAGAG AGACTACAAT 
ACAAAGAGAG TTGACCACTC AAACACAAGA TTAGCCTCTC AGCTTGACAG AAATCCAGGT 
TCAGATGACA ACAACTTGAA TTCCATATTT TATGAGCATC TCACACGCTC CCTTCAGCAT 
AGTCTGTGTG GTGATCTGTT GCTTGGCCGC TGGGGAAATT ACAATACTGG GGATTGCTTT 
ATTCTGGCCT CAGATTATCT AAACGCCTTG GTGCACCTTA TCGAAATAGG CAATGGTCTG 
GTCACCTTCC AGCTAAGAGG ACTAGAGTTC AGAGGTACCT ACTGCCAGCA GCGTGAAGTT 
GAAGCAATCA CTGAGGGTGT AGAGGAAGAT GAAGGCTTTT GCTGTTGCGA ACCTGGACAT 
ATTCCTCATA TGCTGTCTTT CAATGCTGCC TTTGGGCAGC GCTGGCTGGC ATGGGAAGTC 
TTGGTTACAA AGTATGTTCT GGAAGGTTAC AGCATCACAG ACAACAGTGC AGCCTCTATG 
CTTCAAGTGT TTGACCTTCG CAGGATCCTC ACTACCTATT ATGTTAAGGG CATTATATAC 
TATGTGACAG CATCTGCAAA ACTGGAGGAG TGGTTAGCAA ATGAGACAAT GCAGGAAGGT 
CTTCGCCAGT GTTCTGATCA TTGTTATGTT GATGTAGATC CAACTTTCAA CCCAAATATT 
GATGAAGACT ATGACCTTCG CTTAGCAGGC ATTTCACGGG AGAGCTTCTG TGCAAGCTAT 
CTTAACTGGA TTAAGTACTG TTCCTCTAGA AGGGAGAAGC CTCTTGATGC TGAAAAGGAT 
TCACCTTTGG TAACACTTTG TTATGGGCTC TGTATTTTGG GGAGAAGGGC ACTTGGTACA 
GCTTCTCATC ACATGTCCAG CAATTTGGAG TCCTTTCTAT ATGGTTTACA TGCCTTGTTT 
AAAGGAGATT TCCGCATATC ATCTCTCCGT GATGAGTGGA TTTTTGCTGA TATGGAGCTT 
TTAAGAAAAG TAGTAGTTCC TGGCATACGT ATGTCACTAA AACTTCATCA GGACCACTTT 
ACATCTCCAG ATGAGTATGA TGATCCTGCA GTTTTGTATG AAGCCATTAC ATCACATGAG 
CAGAACCTTG TTATTGCGCA TGAGGGAGAT CCAGCGTGGA GGAGTGCTGT TCTTTCTAAC 
TCACCTTCTC TTCTCGCGCT GCGTCATGTG ATGGATGATG GAACCAACGA ATACAAGATC 
ATAATGTTAA ATAAACGTTA TCTTAGTTTT AGAGTCATTA AGGTAAACAA GGAGTGTGTG 
CGGGGCTTAT GGGCTGGTCA GCAGCAAGAA TTGGTCTTTC TTCGTAATCG GAACCCTGAA 
AGAGGCAGCA TCCAAAATGC AAAGCAGGCT CTCAGGAATA TGATCAATTC ATCATGTGAC 
CAACCTATTG GATACCCAAT TTATGTTTCT CCACTGACAA CCTCTTATTC AGACAGCCAT 
GATCAGCTTA AACAGATATT TGGTGGGCCA ATTAGCTTGG CCAATATCCG GAACTTCATT 
GTATCTACAT GGCACAGATT AAGAAAAGGT TGCGGTGCAG GATGCAATAG TGGTGGAAAT 
ATTGAGGACT CTGACACTGG AGGCGGGACC TCCATTAATA GCAACAATCC ACCAAATGCA 
AATGAAACTC AGAGCAGTCT GTCACAAGTT GGTGTCCCAG GTGGAACAGG GCATGTGCCG 
AATGCACGGC TCCAGCCTCC GGGGATGCCT CATCCATCAA CACTAGGAAC CAGTCACAGT 
TCTCACTCCA TGCAGTCAAG CCTTGTCAGA CATTCCCCAG CTCGTGGATC AGTAGCAAGT 
CAATCATCTT ACTGCTACAA CAGCCGTCAC TCATCACTTA GAACATCCAC AACAGGATTT 
GTGCCTTGTA GGCGCTCCTC TACCAGCCAG ATATCTCTTC GCAATCTCCC CTCATCCATT 
CAGTCCCGTC TTTCCATGGT GAACCAAACA GACCCCACAG GACAGTCAGT TTTGGTTGGC 
CTTCCTTCCT CCAGCAGCTC AAGCCAGAGC ATCCCTGCAT GCAAAAGACA CACCTTAGTG 
GGCTTTTTAG GAACAGAAGG AGGCAATAAC CTCACTGAAA CGCAATCCTG TGGCAATTCT 
AGTCCAGCAA CCCATTCAAG AGCCAAAAAG GAAGAGGTTG TTTACAGAGT CCAGATTGTG 
GATCCAAGCC AAGTGCTTGA AGGCGTGAAT ATATCCAAAA GAAAGGAGCT GCAGTGGCCA 
GATGAGCTGG TGCGGCTAAA AGCCGGCAAG AACAGCTGGA AAGACTGGAG CCCTCAGGAA 
GGCATGGAAG GCCATGTAAT TCACCGCTGG GTGCCTTGCA GCAGAGACCC TTGTAATAGG 
TCCCACATTG ACAAAACAAT CCTGCTGATT AAGATTGAGG ACAAATATGT TGCTGTGATT 
GAAACTGGTG TGCTTGAACT CGGAGCTGAA GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004917312.2
CDS	277..7212
Translation	MGSQTLQILRQGVWASITGGWYYDPHQSTFVNTLHLYIWLFLLCFPFTLYMALPTSLVIVAVYCPVVAAVFIVLKLVNYRLHRALDEGEIVERSASELTERVAKAEHSDSSARREDSNGPGDPGGGIEMSEFVREATPPVGCSSRNSYSGLDPSNQVGVSRYGGASGSKDLTVDQKLFRLVSNDSFVSMQPSISSGGKDLTRDFSDKGSIPTHSHHHHVDLSLSNACDTELASLVPLHSHSYRKDHRPRGVPRTSSSAVAFPDTSLTDFSLYQPRRGLDPVSELEASKPHSGSKDSLVDNACASGIFQLDCLKSKGAQQLSRSGSVKHMKTDKSMDSLRSLSTRSSGSTESYCSGTDRDTNSTVSSYKSEQTSSTHVESILSEHEDVTAAKRVHCSDLEEEHSSATDKRTSSIRTALERNTAQEAKDANTSDDVQSPRGLSSSASEEANKNPHANELTVQTDWLPGKLSVNIDEPGEKPVILVDLRSLEETGIKQRDNDVRPKSSSLIHRTSSAHKSCRRRTGKKRASSFDASRHKEYVSFQGIADTKPHSTGFCHDEDSSDQSDLSRTSSVQSAHRFSSDSSSSTTSHSCQSPEARYSALRTKYRIKERGTDSHHTHLNCEGTSKKRSNRRTSSASGTKSRARVLSLDSGTVVSLSDANRLAAPENIKTLTTSKSDLEAKEGEVLDQMSLLGRASQLESVTRSRNSLPSQVTFQDVGERDASVGAHASEETVTFRRERSTFRRQAVRRRHNAGSNPTPPASLIGSPLSLQDGHQGQQSSTQVKVQSRPPSQAAVLSASASLLVRNGSVHLEASHDNASAVGSSSLHDELGMQALCNLGDMEVLMQHILGKFTPSLYETGGCDMSLVNFEPAARRTSNNLCDTDSHVSSSTSVRFYPHDLLSLPQIRLNRLLTIDTDLLEQQDIDLSPDLQDQMQPQEQAAQKVKHYYRFWILPKLWVGINFDRLTLLALFDRNREVLENVLSVILAILVAFLGSVLLIEGFFKDIWVFQFCLVIASCQYSLLKSVQPDSSSPRHGHNRIIAYSRPVYFCACCGLIWLLDYGSKNMSTTKYKLYGMAFTNPLLLVSARDLVIVFTLCFPVVFFIGLLPQVNTFVMYLCEQVDIHVFGGNATTSLLAAIYSFIRSIVAIALLYGLCYGALKDPWDAQHIPVLFSIFCGVLVAVSYHLSRQSSDPSVLISLVQSKIFPNSEDTNPEDPLSEVKDPLPEKLRSSVNERLQSDVVVCVVIAVLYFAIHVSTVFTALQPVLSYVLYALVGSVGLLTHYILPQVRKQLPWHCFSHPLLKTKEYYQFEVRNAAHLMWFEKAHIWLLFIEKNIIYPLIVLNELSIGAKDIVSPKKLDAEVGALLITIAGMKLLRSSFSSPTCQYATVIFTVLFFTFDYKEYTEKLLLDLFFMSILFSKLWELFFKLRFVYTYIAPWQITWGSAFHAFAQPFAVPHSAMLFVQAIVSALFSTPLNPFLGSAIFITSYVRPVKFWERDYNTKRVDHSNTRLASQLDRNPGSDDNNLNSIFYEHLTRSLQHSLCGDLLLGRWGNYNTGDCFILASDYLNALVHLIEIGNGLVTFQLRGLEFRGTYCQQREVEAITEGVEEDEGFCCCEPGHIPHMLSFNAAFGQRWLAWEVLVTKYVLEGYSITDNSAASMLQVFDLRRILTTYYVKGIIYYVTASAKLEEWLANETMQEGLRQCSDHCYVDVDPTFNPNIDEDYDLRLAGISRESFCASYLNWIKYCSSRREKPLDAEKDSPLVTLCYGLCILGRRALGTASHHMSSNLESFLYGLHALFKGDFRISSLRDEWIFADMELLRKVVVPGIRMSLKLHQDHFTSPDEYDDPAVLYEAITSHEQNLVIAHEGDPAWRSAVLSNSPSLLALRHVMDDGTNEYKIIMLNKRYLSFRVIKVNKECVRGLWAGQQQELVFLRNRNPERGSIQNAKQALRNMINSSCDQPIGYPIYVSPLTTSYSDSHDQLKQIFGGPISLANIRNFIVSTWHRLRKGCGAGCNSGGNIEDSDTGGGTSINSNNPPNANETQSSLSQVGVPGGTGHVPNARLQPPGMPHPSTLGTSHSSHSMQSSLVRHSPARGSVASQSSYCYNSRHSSLRTSTTGFVPCRRSSTSQISLRNLPSSIQSRLSMVNQTDPTGQSVLVGLPSSSSSSQSIPACKRHTLVGFLGTEGGNNLTETQSCGNSSPATHSRAKKEEVVYRVQIVDPSQVLEGVNISKRKELQWPDELVRLKAGKNSWKDWSPQEGMEGHVIHRWVPCSRDPCNRSHIDKTILLIKIEDKYVAVIETGVLELGAEV

Target ORF information:

RefSeq Version	XM_004917255.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pecanex 1 (pcnx1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004917255.4

ORF Insert Sequence:

ATGGGTTCCC AGACACTGCA AATCCTCCGG CAGGGAGTCT GGGCTTCCAT CACCGGAGGC 
TGGTACTACG ACCCCCACCA GAGTACCTTT GTCAATACCC TACACCTCTA CATCTGGCTC 
TTCCTCCTCT GCTTCCCCTT CACCCTCTAC ATGGCACTAC CCACATCTTT GGTGATTGTT 
GCTGTGTACT GCCCAGTCGT TGCAGCTGTT TTTATTGTTT TGAAGCTTGT TAACTACCGT 
CTGCACAGGG CATTGGATGA AGGAGAAATT GTGGAGAGGA GTGCTAGTGA GTTGACTGAG 
AGGGTGGCAA AGGCAGAACA CAGTGACAGC TCTGCAAGAA GAGAGGACAG CAATGGACCT 
GGTGATCCAG GTGGAGGGAT TGAAATGTCT GAATTTGTTC GAGAAGCAAC TCCTCCAGTT 
GGATGCAGTT CACGTAATTC CTACTCTGGG TTGGACCCAA GCAATCAGGT TGGTGTGTCT 
CGTTATGGAG GTGCATCTGG AAGCAAAGAT TTGACTGTGG ATCAGAAGCT TTTCCGTCTC 
GTCTCCAATG ACTCCTTTGT CTCCATGCAG CCATCTATAT CCTCGGGTGG AAAAGACTTA 
ACTAGAGACT TCAGTGACAA AGGATCAATC CCAACACACA GTCATCATCA CCATGTGGAT 
CTGTCTCTGT CTAATGCTTG TGACACAGAA TTGGCCTCAC TTGTTCCATT GCATTCACAT 
TCGTACAGGA AAGATCATCG ACCAAGAGGT GTCCCAAGGA CTTCAAGTTC TGCTGTTGCT 
TTCCCAGACA CTTCTCTCAC TGACTTTTCA CTCTATCAAC CAAGGCGCGG GCTGGATCCT 
GTTAGTGAAC TTGAAGCTTC CAAACCACAT TCTGGGTCCA AAGATTCACT AGTAGATAAT 
GCTTGTGCTT CAGGGATTTT TCAATTAGAC TGTCTAAAAA GCAAAGGAGC CCAGCAGCTT 
TCAAGAAGTG GTAGTGTTAA ACACATGAAG ACTGACAAAA GCATGGATAG TTTAAGAAGC 
CTCAGCACGA GAAGCAGTGG ATCCACAGAA AGTTATTGTA GTGGAACAGA TCGGGACACA 
AATAGCACTG TTAGCAGCTA CAAAAGTGAA CAGACCAGTT CAACTCATGT TGAAAGTATT 
TTATCTGAGC ATGAGGATGT TACTGCTGCA AAGAGAGTCC ATTGTAGTGA CCTGGAGGAA 
GAACATAGCT CTGCTACTGA TAAAAGGACT AGTAGTATAA GAACTGCTTT AGAAAGAAAC 
ACTGCTCAGG AAGCCAAGGA TGCCAACACA TCTGATGATG TACAAAGTCC AAGGGGTCTT 
AGCTCATCAG CATCAGAGGA AGCTAATAAG AATCCACACG CAAATGAGTT AACTGTACAA 
ACAGACTGGC TTCCAGGTAA ACTGTCAGTG AACATTGATG AACCTGGGGA GAAACCAGTC 
ATTTTAGTTG ACTTGAGATC TTTGGAAGAA ACGGGTATTA AGCAGAGAGA CAATGACGTA 
CGACCCAAAT CATCTAGTTT AATACATCGA ACATCCTCTG CTCATAAATC ATGCAGGAGA 
CGGACAGGTA AAAAAAGAGC CAGCAGTTTT GATGCAAGTA GACACAAAGA ATATGTTTCC 
TTTCAAGGTA TAGCTGATAC CAAACCTCAC AGTACTGGCT TTTGTCATGA TGAAGATTCT 
AGCGACCAAA GCGACTTGAG CAGGACCTCA AGCGTTCAGT CGGCCCACCG TTTCAGCAGC 
GATAGCTCTT CCAGCACTAC TTCCCATTCA TGCCAGTCTC CTGAAGCAAG ATACAGTGCC 
CTAAGGACAA AATATCGAAT AAAAGAAAGA GGCACAGATT CACATCACAC ACACCTAAAT 
TGTGAAGGAA CTAGTAAAAA GCGGTCCAAC CGCAGGACTT CTAGTGCAAG TGGTACAAAA 
AGCCGAGCTA GAGTATTAAG TCTGGACAGT GGCACTGTAG TTAGTTTGAG TGATGCCAAC 
AGACTGGCCG CTCCTGAAAA TATAAAAACC TTAACCACTT CAAAATCAGA TCTAGAAGCC 
AAAGAAGGCG AAGTGCTAGA TCAGATGTCT CTGTTGGGGC GAGCTTCACA GTTAGAATCT 
GTAACACGGT CAAGAAATAG TTTGCCCAGC CAGGTTACTT TCCAAGATGT TGGAGAGCGA 
GACGCATCTG TTGGAGCACA CGCCAGTGAG GAGACAGTAA CATTTCGCCG CGAACGCAGC 
ACATTTAGGC GCCAAGCGGT TCGGCGCAGA CACAATGCAG GGAGTAATCC CACCCCTCCT 
GCGTCACTCA TCGGATCACC TCTGAGCCTT CAAGATGGTC ATCAAGGCCA GCAGTCATCA 
ACCCAGGTCA AAGTACAGTC CCGCCCCCCT TCCCAGGCTG CAGTGCTCAG CGCTAGCGCC 
TCCTTGCTGG TGAGAAATGG GAGTGTCCAC TTAGAAGCGT CTCATGACAA TGCATCTGCC 
GTAGGCAGTA GCAGTTTGCA CGATGAACTT GGTATGCAGG CCTTATGTAA CCTTGGTGAC 
ATGGAAGTTT TAATGCAGCA CATTTTGGGT AAATTTACCC CATCTCTATA TGAGACTGGA 
GGTTGTGACA TGTCACTTGT AAACTTTGAG CCAGCTGCAA GAAGGACGTC TAATAATCTT 
TGTGACACAG ATTCCCATGT ATCAAGTTCT ACCTCAGTTC GATTCTACCC ACATGATCTA 
CTCTCTCTTC CCCAGATCCG ATTAAACAGG CTGCTAACCA TAGATACAGA TCTGTTAGAG 
CAGCAGGATA TTGATCTAAG CCCTGACCTG CAGGACCAGA TGCAACCACA GGAACAGGCT 
GCCCAAAAAG TAAAACACTA CTACCGGTTT TGGATTCTCC CAAAGCTGTG GGTCGGCATC 
AACTTTGACA GGCTTACACT CTTAGCCTTG TTCGATAGGA ACCGCGAGGT CCTAGAAAAT 
GTGCTGTCTG TCATTCTCGC CATTCTAGTT GCCTTTTTGG GCTCTGTTCT TCTCATAGAA 
GGCTTCTTTA AAGATATCTG GGTCTTCCAG TTTTGTCTTG TTATAGCCAG CTGTCAATAC 
TCTCTCCTTA AGAGTGTTCA ACCAGATTCT TCTTCCCCAA GACATGGCCA TAATCGTATC 
ATTGCCTATA GTAGGCCCGT TTACTTCTGT GCCTGTTGCG GCCTTATTTG GTTATTGGAT 
TATGGAAGCA AAAACATGTC TACCACCAAA TACAAACTAT ATGGAATGGC CTTCACCAAT 
CCTCTGCTTC TAGTGTCAGC CAGGGACTTA GTCATTGTCT TCACACTCTG TTTCCCAGTC 
GTGTTCTTTA TTGGTCTCCT GCCTCAGGTG AACACATTCG TCATGTACCT GTGTGAGCAA 
GTAGATATAC ATGTGTTTGG TGGAAATGCT ACCACCAGCT TGCTTGCAGC AATTTACAGC 
TTTATCCGTA GCATTGTGGC CATAGCTTTA CTCTACGGGC TGTGTTACGG TGCTCTTAAG 
GATCCATGGG ATGCACAACA CATTCCTGTA TTATTCTCTA TTTTCTGTGG TGTTCTTGTG 
GCAGTTTCAT ACCATCTCAG CCGACAGAGC AGCGATCCCT CTGTCCTCAT CTCACTGGTG 
CAATCAAAGA TTTTTCCAAA CAGCGAGGAT ACAAATCCTG AAGACCCACT ATCAGAAGTG 
AAAGACCCTC TTCCAGAAAA ACTGAGAAGC TCTGTTAATG AACGACTTCA GTCAGACGTA 
GTAGTCTGTG TCGTTATTGC AGTGTTGTAC TTTGCAATTC ATGTCAGCAC CGTATTTACT 
GCATTACAGC CGGTTCTGAG CTACGTACTC TATGCACTAG TTGGTTCTGT TGGACTCCTT 
ACTCACTACA TTCTACCACA AGTACGGAAA CAGCTTCCTT GGCACTGCTT CTCCCACCCG 
CTGCTGAAAA CTAAGGAATA TTACCAATTT GAAGTTCGAA ATGCTGCTCA TCTGATGTGG 
TTTGAGAAGG CTCACATCTG GCTGCTTTTT ATCGAGAAGA ACATTATTTA TCCTCTTATA 
GTTCTCAATG AGCTTAGCAT TGGTGCTAAG GATATCGTCA GCCCGAAAAA GCTGGATGCA 
GAAGTTGGTG CATTGTTGAT CACCATTGCC GGAATGAAGC TGCTGAGGTC GTCATTTAGC 
AGTCCCACCT GCCAGTATGC CACTGTCATT TTCACTGTCC TCTTCTTTAC TTTTGACTAC 
AAGGAGTATA CAGAAAAACT GCTGCTAGAT CTGTTCTTTA TGTCAATCCT CTTTAGCAAG 
CTCTGGGAAC TGTTTTTCAA ACTGCGCTTT GTCTACACCT ATATTGCCCC TTGGCAGATC 
ACGTGGGGAT CAGCATTCCA TGCTTTCGCA CAGCCATTTG CTGTACCTCA TTCTGCAATG 
CTGTTTGTAC AGGCAATTGT TTCTGCACTG TTCTCCACTC CCCTGAACCC TTTCTTAGGG 
AGTGCAATCT TTATTACATC ATATGTACGC CCTGTCAAGT TTTGGGAGAG AGACTACAAT 
ACAAAGAGAG TTGACCACTC AAACACAAGA TTAGCCTCTC AGCTTGACAG AAATCCAGGT 
TCAGATGACA ACAACTTGAA TTCCATATTT TATGAGCATC TCACACGCTC CCTTCAGCAT 
AGTCTGTGTG GTGATCTGTT GCTTGGCCGC TGGGGAAATT ACAATACTGG GGATTGCTTT 
ATTCTGGCCT CAGATTATCT AAACGCCTTG GTGCACCTTA TCGAAATAGG CAATGGTCTG 
GTCACCTTCC AGCTAAGAGG ACTAGAGTTC AGAGGTACCT ACTGCCAGCA GCGTGAAGTT 
GAAGCAATCA CTGAGGGTGT AGAGGAAGAT GAAGGCTTTT GCTGTTGCGA ACCTGGACAT 
ATTCCTCATA TGCTGTCTTT CAATGCTGCC TTTGGGCAGC GCTGGCTGGC ATGGGAAGTC 
TTGGTTACAA AGTATGTTCT GGAAGGTTAC AGCATCACAG ACAACAGTGC AGCCTCTATG 
CTTCAAGTGT TTGACCTTCG CAGGATCCTC ACTACCTATT ATGTTAAGGG CATTATATAC 
TATGTGACAG CATCTGCAAA ACTGGAGGAG TGGTTAGCAA ATGAGACAAT GCAGGAAGGT 
CTTCGCCAGT GTTCTGATCA TTGTTATGTT GATGTAGATC CAACTTTCAA CCCAAATATT 
GATGAAGACT ATGACCTTCG CTTAGCAGGC ATTTCACGGG AGAGCTTCTG TGCAAGCTAT 
CTTAACTGGA TTAAGTACTG TTCCTCTAGA AGGGAGAAGC CTCTTGATGC TGAAAAGGAT 
TCACCTTTGG TAACACTTTG TTATGGGCTC TGTATTTTGG GGAGAAGGGC ACTTGGTACA 
GCTTCTCATC ACATGTCCAG CAATTTGGAG TCCTTTCTAT ATGGTTTACA TGCCTTGTTT 
AAAGGAGATT TCCGCATATC ATCTCTCCGT GATGAGTGGA TTTTTGCTGA TATGGAGCTT 
TTAAGAAAAG TAGTAGTTCC TGGCATACGT ATGTCACTAA AACTTCATCA GGACCACTTT 
ACATCTCCAG ATGAGTATGA TGATCCTGCA GTTTTGTATG AAGCCATTAC ATCACATGAG 
CAGAACCTTG TTATTGCGCA TGAGGGAGAT CCAGCGTGGA GGAGTGCTGT TCTTTCTAAC 
TCACCTTCTC TTCTCGCGCT GCGTCATGTG ATGGATGATG GAACCAACGA ATACAAGATC 
ATAATGTTAA ATAAACGTTA TCTTAGTTTT AGAGTCATTA AGGTAAACAA GGAGTGTGTG 
CGGGGCTTAT GGGCTGGTCA GCAGCAAGAA TTGGTCTTTC TTCGTAATCG GAACCCTGAA 
AGAGGCAGCA TCCAAAATGC AAAGCAGGCT CTCAGGAATA TGATCAATTC ATCATGTGAC 
CAACCTATTG GATACCCAAT TTATGTTTCT CCACTGACAA CCTCTTATTC AGACAGCCAT 
GATCAGCTTA AACAGATATT TGGTGGGCCA ATTAGCTTGG CCAATATCCG GAACTTCATT 
GTATCTACAT GGCACAGATT AAGAAAAGGT TGCGGTGCAG GATGCAATAG TGGTGGAAAT 
ATTGAGGACT CTGACACTGG AGGCGGGACC TCCATTAATA GCAACAATCC ACCAAATGCA 
AATGAAACTC AGAGCAGTCT GTCACAAGTT GGTGTCCCAG GTGGAACAGG GCATGTGCCG 
AATGCACGGC TCCAGCCTCC GGGGATGCCT CATCCATCAA CACTAGGAAC CAGTCACAGT 
TCTCACTCCA TGCAGTCAAG CCTTGTCAGA CATTCCCCAG CTCGTGGATC AGTAGCAAGT 
CAATCATCTT ACTGCTACAA CAGCCGTCAC TCATCACTTA GAACATCCAC AACAGGATTT 
GTGCCTTGTA GGCGCTCCTC TACCAGCCAG ATATCTCTTC GCAATCTCCC CTCATCCATT 
CAGTCCCGTC TTTCCATGGT GAACCAAACA GACCCCACAG GACAGTCAGT TTTGGTTGGC 
CTTCCTTCCT CCAGCAGCTC AAGCCAGAGC ATCCCTGCAT GCAAAAGACA CACCTTAGTG 
GGCTTTTTAG GAACAGAAGG AGGCAATAAC CTCACTGAAA CGCAATCCTG TGGCAATTCT 
AGTCCAGCAA CCCATTCAAG AGCCAAAAAG GAAGAGGTTG TTTACAGAGT CCAGATTGTG 
GATCCAAGCC AAGTGCTTGA AGGCGTGAAT ATATCCAAAA GAAAGGAGCT GCAGTGGCCA 
GATGAGCTGG TGCGGCTAAA AGCCGGCAAG AACAGCTGGA AAGACTGGAG CCCTCAGGAA 
GGCATGGAAG GCCATGTAAT TCACCGCTGG GTGCCTTGCA GCAGAGACCC TTGTAATAGG 
TCCCACATTG ACAAAACAAT CCTGCTGATT AAGATTGAGG ACAAATATGT TGCTGTGATT 
GAAACTGGTG TGCTTGAACT CGGAGCTGAA GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pcnx1 ( XM_012969000.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012824454.2 Xenopus tropicalis pecanex 1 (pcnx1), transcript variant X6, mRNA.

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CloneID	OXa64212
Clone ID Related Accession (Same CDS sequence)	XM_012969000.3
Accession Version	XM_012969000.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pecanex-like protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012969000.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCC AGACACTGCA AATCCTCCGG CAGGGAGTCT GGGCTTCCAT CACCGGAGGC 
TGGTACTACG ACCCCCACCA GAGTACCTTT GTCAATACCC TACACCTCTA CATCTGGCTC 
TTCCTCCTCT GCTTCCCCTT CACCCTCTAC ATGGCACTAC CCACATCTTT GGTGATTGTT 
GCTGTGTACT GCCCAGTCGT TGCAGCTGTT TTTATTGTTT TGAAGCTTGT TAACTACCGT 
CTGCACAGGG CATTGGATGA AGGAGAAATT GTGGAGAGGA GTGCTAGTGA GTTGACTGAG 
AGGGTGGCAA AGGCAGAACA CAGTGACAGC TCTGCAAGAA GAGAGGACAG CAATGGACCT 
GGTGATCCAG GTGGAGGGAT TGAAATGTCT GAATTTGTTC GAGAAGCAAC TCCTCCAGTT 
GGATGCAGTT CACGTAATTC CTACTCTGGG TTGGACCCAA GCAATCAGGT TGGTGTGTCT 
CGTTATGGAG GTGCATCTGG AAGCAAAGGG GATACAGATG CTGCAAAGAC ATCAGATGAT 
ATCAGTCTGA GTCTTGGCCA GAGTTACAGT TTGTGCAAAG AAGCAAGTGA AGACCAAGAT 
TTGACTGTGG ATCAGAAGCT TTTCCGTCTC GTCTCCAATG ACTCCTTTGT CTCCATGCAG 
CCATCTATAT CCTCGGGTGG AAAAGACTTA ACTAGAGACT TCAGTGACAA AGGATCAATC 
CCAACACACA GTCATCATCA CCATGTGGAT CTGTCTCTGT CTAATGCTTG TGACACAGAA 
TTGGCCTCAC TTGTTCCATT GCATTCACAT TCGTACAGGA AAGATCATCG ACCAAGAGGT 
GTCCCAAGGA CTTCAAGTTC TGCTGTTGCT TTCCCAGACA CTTCTCTCAC TGACTTTTCA 
CTCTATCAAC CAAGGCGCGG GCTGGATCCT GTTAGTGAAC TTGAAGCTTC CAAACCACAT 
TCTGGGTCCA AAGATTCACT AGTAGATAAT GCTTGTGCTT CAGGGATTTT TCAATTAGAC 
TGTCTAAAAA GCAAAGGAGC CCAGCAGCTT TCAAGAAGTG GTAGTGTTAA ACACATGAAG 
ACTGACAAAA GCATGGATAG TTTAAGAAGC CTCAGCACGA GAAGCAGTGG ATCCACAGAA 
AGTTATTGTA GTGGAACAGA TCGGGACACA AATAGCACTG TTAGCAGCTA CAAAAGTGAA 
CAGACCAGTT CAACTCATGT TGAAAGTATT TTATCTGAGC ATGAGGATGT TACTGCTGCA 
AAGAGAGTCC ATTGTAGTGA CCTGGAGGAA GAACATAGCT CTGCTACTGA TAAAAGGACT 
AGTAGTATAA GAACTGCTTT AGAAAGAAAC ACTGCTCAGG AAGCCAAGGA TGCCAACACA 
TCTGATGATG TACAAAGTCC AAGGGGTCTT AGCTCATCAG CATCAGAGGA AGCTAATAAG 
AATCCACACG CAAATGAGTT AACTGTACAA ACAGACTGGC TTCCAGGTAA ACTGTCAGTG 
AACATTGATG AACCTGGGGA GAAACCAGTC ATTTTAGTTG ACTTGAGATC TTTGGAAGAA 
ACGGGTATTA AGCAGAGAGA CAATGACGTA CGACCCAAAT CATCTAGTTT AATACATCGA 
ACATCCTCTG CTCATAAATC ATGCAGGAGA CGGACAGGTA AAAAAAGAGC CAGCAGTTTT 
GATGCAAGTA GACACAAAGA ATATGTTTCC TTTCAAGGTA TAGCTGATAC CAAACCTCAC 
AGTACTGGCT TTTGTCATGA TGAAGATTCT AGCGACCAAA GCGACTTGAG CAGGACCTCA 
AGCGTTCAGT CGGCCCACCG TTTCAGCAGC GATAGCTCTT CCAGCACTAC TTCCCATTCA 
TGCCAGTCTC CTGAAGCAAG ATACAGTGCC CTAAGGACAA AATATCGAAT AAAAGAAAGA 
GGCACAGATT CACATCACAC ACACCTAAAT TGTGAAGGAA CTAGTAAAAA GCGGTCCAAC 
CGCAGGACTT CTAGTGCAAG TGGTACAAAA AGCCGAGCTA GAGTATTAAG TCTGGACAGT 
GGCACTGTAG TTAGTTTGAG TGATGCCAAC AGACTGGCCG CTCCTGAAAA TATAAAAACC 
TTAACCACTT CAAAATCAGA TCTAGAAGCC AAAGAAGGCG AAGTGCTAGA TCAGATGTCT 
CTGTTGGGGC GAGCTTCACA GTTAGAATCT GTAACACGGT CAAGAAATAG TTTGCCCAGC 
CAGGTTACTT TCCAAGATGT TGGAGAGCGA GACGCATCTG TTGGAGGTAA ATTTACCCCA 
TCTCTATATG AGACTGGAGG TTGTGACATG TCACTTGTAA ACTTTGAGCC AGCTGCAAGA 
AGGACGTCTA ATAATCTTTG TGACACAGAT TCCCATGTAT CAAGTTCTAC CTCAGTTCGA 
TTCTACCCAC ATGATCTACT CTCTCTTCCC CAGATCCGAT TAAACAGGCT GCTAACCATA 
GATACAGATC TGTTAGAGCA GCAGGATATT GATCTAAGCC CTGACCTGCA GGACCAGATG 
CAACCACAGG AACAGGCTGC CCAAAAAGTA AAACACTACT ACCGGTTTTG GATTCTCCCA 
AAGCTGTGGG TCGGCATCAA CTTTGACAGG CTTACACTCT TAGCCTTGTT CGATAGGAAC 
CGCGAGGTCC TAGAAAATGT GCTGTCTGTC ATTCTCGCCA TTCTAGTTGC CTTTTTGGGC 
TCTGTTCTTC TCATAGAAGG CTTCTTTAAA GATATCTGGG TCTTCCAGTT TTGTCTTGTT 
ATAGCCAGCT GTCAATACTC TCTCCTTAAG AGTGTTCAAC CAGATTCTTC TTCCCCAAGA 
CATGGCCATA ATCGTATCAT TGCCTATAGT AGGCCCGTTT ACTTCTGTGC CTGTTGCGGC 
CTTATTTGGT TATTGGATTA TGGAAGCAAA AACATGTCTA CCACCAAATA CAAACTATAT 
GGAATGGCCT TCACCAATCC TCTGCTTCTA GTGTCAGCCA GGGACTTAGT CATTGTCTTC 
ACACTCTGTT TCCCAGTCGT GTTCTTTATT GGTCTCCTGC CTCAGGTGAA CACATTCGTC 
ATGTACCTGT GTGAGCAAGT AGATATACAT GTGTTTGGTG GAAATGCTAC CACCAGCTTG 
CTTGCAGCAA TTTACAGCTT TATCCGTAGC ATTGTGGCCA TAGCTTTACT CTACGGGCTG 
TGTTACGGTG CTCTTAAGGA TCCATGGGAT GCACAACACA TTCCTGTATT ATTCTCTATT 
TTCTGTGGTG TTCTTGTGGC AGTTTCATAC CATCTCAGCC GACAGAGCAG CGATCCCTCT 
GTCCTCATCT CACTGGTGCA ATCAAAGATT TTTCCAAACA GCGAGGATAC AAATCCTGAA 
GACCCACTAT CAGAAGTGAA AGACCCTCTT CCAGAAAAAC TGAGAAGCTC TGTTAATGAA 
CGACTTCAGT CAGACGTAGT AGTCTGTGTC GTTATTGCAG TGTTGTACTT TGCAATTCAT 
GTCAGCACCG TATTTACTGC ATTACAGCCG GTTCTGAGCT ACGTACTCTA TGCACTAGTT 
GGTTCTGTTG GACTCCTTAC TCACTACATT CTACCACAAG TACGGAAACA GCTTCCTTGG 
CACTGCTTCT CCCACCCGCT GCTGAAAACT AAGGAATATT ACCAATTTGA AGTTCGAAAT 
GCTGCTCATC TGATGTGGTT TGAGAAGGCT CACATCTGGC TGCTTTTTAT CGAGAAGAAC 
ATTATTTATC CTCTTATAGT TCTCAATGAG CTTAGCATTG GTGCTAAGGA TATCGTCAGC 
CCGAAAAAGC TGGATGCAGA AGTTGGTGCA TTGTTGATCA CCATTGCCGG AATGAAGCTG 
CTGAGGTCGT CATTTAGCAG TCCCACCTGC CAGTATGCCA CTGTCATTTT CACTGTCCTC 
TTCTTTACTT TTGACTACAA GGAGTATACA GAAAAACTGC TGCTAGATCT GTTCTTTATG 
TCAATCCTCT TTAGCAAGCT CTGGGAACTG TTTTTCAAAC TGCGCTTTGT CTACACCTAT 
ATTGCCCCTT GGCAGATCAC GTGGGGATCA GCATTCCATG CTTTCGCACA GCCATTTGCT 
GTACCTCATT CTGCAATGCT GTTTGTACAG GCAATTGTTT CTGCACTGTT CTCCACTCCC 
CTGAACCCTT TCTTAGGGAG TGCAATCTTT ATTACATCAT ATGTACGCCC TGTCAAGTTT 
TGGGAGAGAG ACTACAATAC AAAGAGAGTT GACCACTCAA ACACAAGATT AGCCTCTCAG 
CTTGACAGAA ATCCAGGTTC AGATGACAAC AACTTGAATT CCATATTTTA TGAGCATCTC 
ACACGCTCCC TTCAGCATAG TCTGTGTGGT GATCTGTTGC TTGGCCGCTG GGGAAATTAC 
AATACTGGGG ATTGCTTTAT TCTGGCCTCA GATTATCTAA ACGCCTTGGT GCACCTTATC 
GAAATAGGCA ATGGTCTGGT CACCTTCCAG CTAAGAGGAC TAGAGTTCAG AGGTACCTAC 
TGCCAGCAGC GTGAAGTTGA AGCAATCACT GAGGGTGTAG AGGAAGATGA AGGCTTTTGC 
TGTTGCGAAC CTGGACATAT TCCTCATATG CTGTCTTTCA ATGCTGCCTT TGGGCAGCGC 
TGGCTGGCAT GGGAAGTCTT GGTTACAAAG TATGTTCTGG AAGGTTACAG CATCACAGAC 
AACAGTGCAG CCTCTATGCT TCAAGTGTTT GACCTTCGCA GGATCCTCAC TACCTATTAT 
GTTAAGGGCA TTATATACTA TGTGACAGCA TCTGCAAAAC TGGAGGAGTG GTTAGCAAAT 
GAGACAATGC AGGAAGGTCT TCGCCAGTGT TCTGATCATT GTTATGTTGA TGTAGATCCA 
ACTTTCAACC CAAATATTGA TGAAGACTAT GACCTTCGCT TAGCAGGCAT TTCACGGGAG 
AGCTTCTGTG CAAGCTATCT TAACTGGATT AAGTACTGTT CCTCTAGAAG GGAGAAGCCT 
CTTGATGCTG AAAAGGATTC ACCTTTGGTA ACACTTTGTT ATGGGCTCTG TATTTTGGGG 
AGAAGGGCAC TTGGTACAGC TTCTCATCAC ATGTCCAGCA ATTTGGAGTC CTTTCTATAT 
GGTTTACATG CCTTGTTTAA AGGAGATTTC CGCATATCAT CTCTCCGTGA TGAGTGGATT 
TTTGCTGATA TGGAGCTTTT AAGAAAAGTA GTAGTTCCTG GCATACGTAT GTCACTAAAA 
CTTCATCAGG ACCACTTTAC ATCTCCAGAT GAGTATGATG ATCCTGCAGT TTTGTATGAA 
GCCATTACAT CACATGAGCA GAACCTTGTT ATTGCGCATG AGGGAGATCC AGCGTGGAGG 
AGTGCTGTTC TTTCTAACTC ACCTTCTCTT CTCGCGCTGC GTCATGTGAT GGATGATGGA 
ACCAACGAAT ACAAGATCAT AATGTTAAAT AAACGTTATC TTAGTTTTAG AGTCATTAAG 
GTAAACAAGG AGTGTGTGCG GGGCTTATGG GCTGGTCAGC AGCAAGAATT GGTCTTTCTT 
CGTAATCGGA ACCCTGAAAG AGGCAGCATC CAAAATGCAA AGCAGGCTCT CAGGAATATG 
ATCAATTCAT CATGTGACCA ACCTATTGGA TACCCAATTT ATGTTTCTCC ACTGACAACC 
TCTTATTCAG ACAGCCATGA TCAGCTTAAA CAGATATTTG GTGGGCCAAT TAGCTTGGCC 
AATATCCGGA ACTTCATTGT ATCTACATGG CACAGATTAA GAAAAGGTTG CGGTGCAGGA 
TGCAATAGTG GTGGAAATAT TGAGGACTCT GACACTGGAG GCGGGACCTC CATTAATAGC 
AACAATCCAC CAAATGCAAA TGAAACTCAG AGCAGTCTGT CACAAGTTGG TGTCCCAGGT 
GGAACAGGGC ATGTGCCGAA TGCACGGCTC CAGCCTCCGG GGATGCCTCA TCCATCAACA 
CTAGGAACCA GTCACAGTTC TCACTCCATG CAGTCAAGCC TTGTCAGACA TTCCCCAGCT 
CGTGGATCAG TAGCAAGTCA ATCATCTTAC TGCTACAACA GCCGTCACTC ATCACTTAGA 
ACATCCACAA CAGGATTTGT GCCTTGTAGG CGCTCCTCTA CCAGCCAGAT ATCTCTTCGC 
AATCTCCCCT CATCCATTCA GTCCCGTCTT TCCATGGTGA ACCAAACAGA CCCCACAGGA 
CAGTCAGTTT TGGTTGGCCT TCCTTCCTCC AGCAGCTCAA GCCAGAGCAT CCCTGCATGC 
AAAAGACACA CCTTAGTGGG CTTTTTAGGA ACAGAAGGAG GCAATAACCT CACTGAAACG 
CAATCCTGTG GCAATTCTAG TCCAGCAACC CATTCAAGAG CCAAAAAGGA AGAGGTTGTT 
TACAGAGTCC AGATTGTGGA TCCAAGCCAA GTGCTTGAAG GCGTGAATAT ATCCAAAAGA 
AAGGAGCTGC AGTGGCCAGA TGAGCTGGTG CGGCTAAAAG CCGGCAAGAA CAGCTGGAAA 
GACTGGAGCC CTCAGGAAGG CATGGAAGGC CATGTAATTC ACCGCTGGGT GCCTTGCAGC 
AGAGACCCTT GTAATAGGTC CCACATTGAC AAAACAATCC TGCTGATTAA GATTGAGGAC 
AAATATGTTG CTGTGATTGA AACTGGTGTG CTTGAACTCG GAGCTGAAGT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012824454.2
CDS	276..6929
Translation	MGSQTLQILRQGVWASITGGWYYDPHQSTFVNTLHLYIWLFLLCFPFTLYMALPTSLVIVAVYCPVVAAVFIVLKLVNYRLHRALDEGEIVERSASELTERVAKAEHSDSSARREDSNGPGDPGGGIEMSEFVREATPPVGCSSRNSYSGLDPSNQVGVSRYGGASGSKGDTDAAKTSDDISLSLGQSYSLCKEASEDQDLTVDQKLFRLVSNDSFVSMQPSISSGGKDLTRDFSDKGSIPTHSHHHHVDLSLSNACDTELASLVPLHSHSYRKDHRPRGVPRTSSSAVAFPDTSLTDFSLYQPRRGLDPVSELEASKPHSGSKDSLVDNACASGIFQLDCLKSKGAQQLSRSGSVKHMKTDKSMDSLRSLSTRSSGSTESYCSGTDRDTNSTVSSYKSEQTSSTHVESILSEHEDVTAAKRVHCSDLEEEHSSATDKRTSSIRTALERNTAQEAKDANTSDDVQSPRGLSSSASEEANKNPHANELTVQTDWLPGKLSVNIDEPGEKPVILVDLRSLEETGIKQRDNDVRPKSSSLIHRTSSAHKSCRRRTGKKRASSFDASRHKEYVSFQGIADTKPHSTGFCHDEDSSDQSDLSRTSSVQSAHRFSSDSSSSTTSHSCQSPEARYSALRTKYRIKERGTDSHHTHLNCEGTSKKRSNRRTSSASGTKSRARVLSLDSGTVVSLSDANRLAAPENIKTLTTSKSDLEAKEGEVLDQMSLLGRASQLESVTRSRNSLPSQVTFQDVGERDASVGGKFTPSLYETGGCDMSLVNFEPAARRTSNNLCDTDSHVSSSTSVRFYPHDLLSLPQIRLNRLLTIDTDLLEQQDIDLSPDLQDQMQPQEQAAQKVKHYYRFWILPKLWVGINFDRLTLLALFDRNREVLENVLSVILAILVAFLGSVLLIEGFFKDIWVFQFCLVIASCQYSLLKSVQPDSSSPRHGHNRIIAYSRPVYFCACCGLIWLLDYGSKNMSTTKYKLYGMAFTNPLLLVSARDLVIVFTLCFPVVFFIGLLPQVNTFVMYLCEQVDIHVFGGNATTSLLAAIYSFIRSIVAIALLYGLCYGALKDPWDAQHIPVLFSIFCGVLVAVSYHLSRQSSDPSVLISLVQSKIFPNSEDTNPEDPLSEVKDPLPEKLRSSVNERLQSDVVVCVVIAVLYFAIHVSTVFTALQPVLSYVLYALVGSVGLLTHYILPQVRKQLPWHCFSHPLLKTKEYYQFEVRNAAHLMWFEKAHIWLLFIEKNIIYPLIVLNELSIGAKDIVSPKKLDAEVGALLITIAGMKLLRSSFSSPTCQYATVIFTVLFFTFDYKEYTEKLLLDLFFMSILFSKLWELFFKLRFVYTYIAPWQITWGSAFHAFAQPFAVPHSAMLFVQAIVSALFSTPLNPFLGSAIFITSYVRPVKFWERDYNTKRVDHSNTRLASQLDRNPGSDDNNLNSIFYEHLTRSLQHSLCGDLLLGRWGNYNTGDCFILASDYLNALVHLIEIGNGLVTFQLRGLEFRGTYCQQREVEAITEGVEEDEGFCCCEPGHIPHMLSFNAAFGQRWLAWEVLVTKYVLEGYSITDNSAASMLQVFDLRRILTTYYVKGIIYYVTASAKLEEWLANETMQEGLRQCSDHCYVDVDPTFNPNIDEDYDLRLAGISRESFCASYLNWIKYCSSRREKPLDAEKDSPLVTLCYGLCILGRRALGTASHHMSSNLESFLYGLHALFKGDFRISSLRDEWIFADMELLRKVVVPGIRMSLKLHQDHFTSPDEYDDPAVLYEAITSHEQNLVIAHEGDPAWRSAVLSNSPSLLALRHVMDDGTNEYKIIMLNKRYLSFRVIKVNKECVRGLWAGQQQELVFLRNRNPERGSIQNAKQALRNMINSSCDQPIGYPIYVSPLTTSYSDSHDQLKQIFGGPISLANIRNFIVSTWHRLRKGCGAGCNSGGNIEDSDTGGGTSINSNNPPNANETQSSLSQVGVPGGTGHVPNARLQPPGMPHPSTLGTSHSSHSMQSSLVRHSPARGSVASQSSYCYNSRHSSLRTSTTGFVPCRRSSTSQISLRNLPSSIQSRLSMVNQTDPTGQSVLVGLPSSSSSSQSIPACKRHTLVGFLGTEGGNNLTETQSCGNSSPATHSRAKKEEVVYRVQIVDPSQVLEGVNISKRKELQWPDELVRLKAGKNSWKDWSPQEGMEGHVIHRWVPCSRDPCNRSHIDKTILLIKIEDKYVAVIETGVLELGAEV

Target ORF information:

RefSeq Version	XM_012969000.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pecanex 1 (pcnx1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012969000.3

ORF Insert Sequence:

ATGGGTTCCC AGACACTGCA AATCCTCCGG CAGGGAGTCT GGGCTTCCAT CACCGGAGGC 
TGGTACTACG ACCCCCACCA GAGTACCTTT GTCAATACCC TACACCTCTA CATCTGGCTC 
TTCCTCCTCT GCTTCCCCTT CACCCTCTAC ATGGCACTAC CCACATCTTT GGTGATTGTT 
GCTGTGTACT GCCCAGTCGT TGCAGCTGTT TTTATTGTTT TGAAGCTTGT TAACTACCGT 
CTGCACAGGG CATTGGATGA AGGAGAAATT GTGGAGAGGA GTGCTAGTGA GTTGACTGAG 
AGGGTGGCAA AGGCAGAACA CAGTGACAGC TCTGCAAGAA GAGAGGACAG CAATGGACCT 
GGTGATCCAG GTGGAGGGAT TGAAATGTCT GAATTTGTTC GAGAAGCAAC TCCTCCAGTT 
GGATGCAGTT CACGTAATTC CTACTCTGGG TTGGACCCAA GCAATCAGGT TGGTGTGTCT 
CGTTATGGAG GTGCATCTGG AAGCAAAGGG GATACAGATG CTGCAAAGAC ATCAGATGAT 
ATCAGTCTGA GTCTTGGCCA GAGTTACAGT TTGTGCAAAG AAGCAAGTGA AGACCAAGAT 
TTGACTGTGG ATCAGAAGCT TTTCCGTCTC GTCTCCAATG ACTCCTTTGT CTCCATGCAG 
CCATCTATAT CCTCGGGTGG AAAAGACTTA ACTAGAGACT TCAGTGACAA AGGATCAATC 
CCAACACACA GTCATCATCA CCATGTGGAT CTGTCTCTGT CTAATGCTTG TGACACAGAA 
TTGGCCTCAC TTGTTCCATT GCATTCACAT TCGTACAGGA AAGATCATCG ACCAAGAGGT 
GTCCCAAGGA CTTCAAGTTC TGCTGTTGCT TTCCCAGACA CTTCTCTCAC TGACTTTTCA 
CTCTATCAAC CAAGGCGCGG GCTGGATCCT GTTAGTGAAC TTGAAGCTTC CAAACCACAT 
TCTGGGTCCA AAGATTCACT AGTAGATAAT GCTTGTGCTT CAGGGATTTT TCAATTAGAC 
TGTCTAAAAA GCAAAGGAGC CCAGCAGCTT TCAAGAAGTG GTAGTGTTAA ACACATGAAG 
ACTGACAAAA GCATGGATAG TTTAAGAAGC CTCAGCACGA GAAGCAGTGG ATCCACAGAA 
AGTTATTGTA GTGGAACAGA TCGGGACACA AATAGCACTG TTAGCAGCTA CAAAAGTGAA 
CAGACCAGTT CAACTCATGT TGAAAGTATT TTATCTGAGC ATGAGGATGT TACTGCTGCA 
AAGAGAGTCC ATTGTAGTGA CCTGGAGGAA GAACATAGCT CTGCTACTGA TAAAAGGACT 
AGTAGTATAA GAACTGCTTT AGAAAGAAAC ACTGCTCAGG AAGCCAAGGA TGCCAACACA 
TCTGATGATG TACAAAGTCC AAGGGGTCTT AGCTCATCAG CATCAGAGGA AGCTAATAAG 
AATCCACACG CAAATGAGTT AACTGTACAA ACAGACTGGC TTCCAGGTAA ACTGTCAGTG 
AACATTGATG AACCTGGGGA GAAACCAGTC ATTTTAGTTG ACTTGAGATC TTTGGAAGAA 
ACGGGTATTA AGCAGAGAGA CAATGACGTA CGACCCAAAT CATCTAGTTT AATACATCGA 
ACATCCTCTG CTCATAAATC ATGCAGGAGA CGGACAGGTA AAAAAAGAGC CAGCAGTTTT 
GATGCAAGTA GACACAAAGA ATATGTTTCC TTTCAAGGTA TAGCTGATAC CAAACCTCAC 
AGTACTGGCT TTTGTCATGA TGAAGATTCT AGCGACCAAA GCGACTTGAG CAGGACCTCA 
AGCGTTCAGT CGGCCCACCG TTTCAGCAGC GATAGCTCTT CCAGCACTAC TTCCCATTCA 
TGCCAGTCTC CTGAAGCAAG ATACAGTGCC CTAAGGACAA AATATCGAAT AAAAGAAAGA 
GGCACAGATT CACATCACAC ACACCTAAAT TGTGAAGGAA CTAGTAAAAA GCGGTCCAAC 
CGCAGGACTT CTAGTGCAAG TGGTACAAAA AGCCGAGCTA GAGTATTAAG TCTGGACAGT 
GGCACTGTAG TTAGTTTGAG TGATGCCAAC AGACTGGCCG CTCCTGAAAA TATAAAAACC 
TTAACCACTT CAAAATCAGA TCTAGAAGCC AAAGAAGGCG AAGTGCTAGA TCAGATGTCT 
CTGTTGGGGC GAGCTTCACA GTTAGAATCT GTAACACGGT CAAGAAATAG TTTGCCCAGC 
CAGGTTACTT TCCAAGATGT TGGAGAGCGA GACGCATCTG TTGGAGGTAA ATTTACCCCA 
TCTCTATATG AGACTGGAGG TTGTGACATG TCACTTGTAA ACTTTGAGCC AGCTGCAAGA 
AGGACGTCTA ATAATCTTTG TGACACAGAT TCCCATGTAT CAAGTTCTAC CTCAGTTCGA 
TTCTACCCAC ATGATCTACT CTCTCTTCCC CAGATCCGAT TAAACAGGCT GCTAACCATA 
GATACAGATC TGTTAGAGCA GCAGGATATT GATCTAAGCC CTGACCTGCA GGACCAGATG 
CAACCACAGG AACAGGCTGC CCAAAAAGTA AAACACTACT ACCGGTTTTG GATTCTCCCA 
AAGCTGTGGG TCGGCATCAA CTTTGACAGG CTTACACTCT TAGCCTTGTT CGATAGGAAC 
CGCGAGGTCC TAGAAAATGT GCTGTCTGTC ATTCTCGCCA TTCTAGTTGC CTTTTTGGGC 
TCTGTTCTTC TCATAGAAGG CTTCTTTAAA GATATCTGGG TCTTCCAGTT TTGTCTTGTT 
ATAGCCAGCT GTCAATACTC TCTCCTTAAG AGTGTTCAAC CAGATTCTTC TTCCCCAAGA 
CATGGCCATA ATCGTATCAT TGCCTATAGT AGGCCCGTTT ACTTCTGTGC CTGTTGCGGC 
CTTATTTGGT TATTGGATTA TGGAAGCAAA AACATGTCTA CCACCAAATA CAAACTATAT 
GGAATGGCCT TCACCAATCC TCTGCTTCTA GTGTCAGCCA GGGACTTAGT CATTGTCTTC 
ACACTCTGTT TCCCAGTCGT GTTCTTTATT GGTCTCCTGC CTCAGGTGAA CACATTCGTC 
ATGTACCTGT GTGAGCAAGT AGATATACAT GTGTTTGGTG GAAATGCTAC CACCAGCTTG 
CTTGCAGCAA TTTACAGCTT TATCCGTAGC ATTGTGGCCA TAGCTTTACT CTACGGGCTG 
TGTTACGGTG CTCTTAAGGA TCCATGGGAT GCACAACACA TTCCTGTATT ATTCTCTATT 
TTCTGTGGTG TTCTTGTGGC AGTTTCATAC CATCTCAGCC GACAGAGCAG CGATCCCTCT 
GTCCTCATCT CACTGGTGCA ATCAAAGATT TTTCCAAACA GCGAGGATAC AAATCCTGAA 
GACCCACTAT CAGAAGTGAA AGACCCTCTT CCAGAAAAAC TGAGAAGCTC TGTTAATGAA 
CGACTTCAGT CAGACGTAGT AGTCTGTGTC GTTATTGCAG TGTTGTACTT TGCAATTCAT 
GTCAGCACCG TATTTACTGC ATTACAGCCG GTTCTGAGCT ACGTACTCTA TGCACTAGTT 
GGTTCTGTTG GACTCCTTAC TCACTACATT CTACCACAAG TACGGAAACA GCTTCCTTGG 
CACTGCTTCT CCCACCCGCT GCTGAAAACT AAGGAATATT ACCAATTTGA AGTTCGAAAT 
GCTGCTCATC TGATGTGGTT TGAGAAGGCT CACATCTGGC TGCTTTTTAT CGAGAAGAAC 
ATTATTTATC CTCTTATAGT TCTCAATGAG CTTAGCATTG GTGCTAAGGA TATCGTCAGC 
CCGAAAAAGC TGGATGCAGA AGTTGGTGCA TTGTTGATCA CCATTGCCGG AATGAAGCTG 
CTGAGGTCGT CATTTAGCAG TCCCACCTGC CAGTATGCCA CTGTCATTTT CACTGTCCTC 
TTCTTTACTT TTGACTACAA GGAGTATACA GAAAAACTGC TGCTAGATCT GTTCTTTATG 
TCAATCCTCT TTAGCAAGCT CTGGGAACTG TTTTTCAAAC TGCGCTTTGT CTACACCTAT 
ATTGCCCCTT GGCAGATCAC GTGGGGATCA GCATTCCATG CTTTCGCACA GCCATTTGCT 
GTACCTCATT CTGCAATGCT GTTTGTACAG GCAATTGTTT CTGCACTGTT CTCCACTCCC 
CTGAACCCTT TCTTAGGGAG TGCAATCTTT ATTACATCAT ATGTACGCCC TGTCAAGTTT 
TGGGAGAGAG ACTACAATAC AAAGAGAGTT GACCACTCAA ACACAAGATT AGCCTCTCAG 
CTTGACAGAA ATCCAGGTTC AGATGACAAC AACTTGAATT CCATATTTTA TGAGCATCTC 
ACACGCTCCC TTCAGCATAG TCTGTGTGGT GATCTGTTGC TTGGCCGCTG GGGAAATTAC 
AATACTGGGG ATTGCTTTAT TCTGGCCTCA GATTATCTAA ACGCCTTGGT GCACCTTATC 
GAAATAGGCA ATGGTCTGGT CACCTTCCAG CTAAGAGGAC TAGAGTTCAG AGGTACCTAC 
TGCCAGCAGC GTGAAGTTGA AGCAATCACT GAGGGTGTAG AGGAAGATGA AGGCTTTTGC 
TGTTGCGAAC CTGGACATAT TCCTCATATG CTGTCTTTCA ATGCTGCCTT TGGGCAGCGC 
TGGCTGGCAT GGGAAGTCTT GGTTACAAAG TATGTTCTGG AAGGTTACAG CATCACAGAC 
AACAGTGCAG CCTCTATGCT TCAAGTGTTT GACCTTCGCA GGATCCTCAC TACCTATTAT 
GTTAAGGGCA TTATATACTA TGTGACAGCA TCTGCAAAAC TGGAGGAGTG GTTAGCAAAT 
GAGACAATGC AGGAAGGTCT TCGCCAGTGT TCTGATCATT GTTATGTTGA TGTAGATCCA 
ACTTTCAACC CAAATATTGA TGAAGACTAT GACCTTCGCT TAGCAGGCAT TTCACGGGAG 
AGCTTCTGTG CAAGCTATCT TAACTGGATT AAGTACTGTT CCTCTAGAAG GGAGAAGCCT 
CTTGATGCTG AAAAGGATTC ACCTTTGGTA ACACTTTGTT ATGGGCTCTG TATTTTGGGG 
AGAAGGGCAC TTGGTACAGC TTCTCATCAC ATGTCCAGCA ATTTGGAGTC CTTTCTATAT 
GGTTTACATG CCTTGTTTAA AGGAGATTTC CGCATATCAT CTCTCCGTGA TGAGTGGATT 
TTTGCTGATA TGGAGCTTTT AAGAAAAGTA GTAGTTCCTG GCATACGTAT GTCACTAAAA 
CTTCATCAGG ACCACTTTAC ATCTCCAGAT GAGTATGATG ATCCTGCAGT TTTGTATGAA 
GCCATTACAT CACATGAGCA GAACCTTGTT ATTGCGCATG AGGGAGATCC AGCGTGGAGG 
AGTGCTGTTC TTTCTAACTC ACCTTCTCTT CTCGCGCTGC GTCATGTGAT GGATGATGGA 
ACCAACGAAT ACAAGATCAT AATGTTAAAT AAACGTTATC TTAGTTTTAG AGTCATTAAG 
GTAAACAAGG AGTGTGTGCG GGGCTTATGG GCTGGTCAGC AGCAAGAATT GGTCTTTCTT 
CGTAATCGGA ACCCTGAAAG AGGCAGCATC CAAAATGCAA AGCAGGCTCT CAGGAATATG 
ATCAATTCAT CATGTGACCA ACCTATTGGA TACCCAATTT ATGTTTCTCC ACTGACAACC 
TCTTATTCAG ACAGCCATGA TCAGCTTAAA CAGATATTTG GTGGGCCAAT TAGCTTGGCC 
AATATCCGGA ACTTCATTGT ATCTACATGG CACAGATTAA GAAAAGGTTG CGGTGCAGGA 
TGCAATAGTG GTGGAAATAT TGAGGACTCT GACACTGGAG GCGGGACCTC CATTAATAGC 
AACAATCCAC CAAATGCAAA TGAAACTCAG AGCAGTCTGT CACAAGTTGG TGTCCCAGGT 
GGAACAGGGC ATGTGCCGAA TGCACGGCTC CAGCCTCCGG GGATGCCTCA TCCATCAACA 
CTAGGAACCA GTCACAGTTC TCACTCCATG CAGTCAAGCC TTGTCAGACA TTCCCCAGCT 
CGTGGATCAG TAGCAAGTCA ATCATCTTAC TGCTACAACA GCCGTCACTC ATCACTTAGA 
ACATCCACAA CAGGATTTGT GCCTTGTAGG CGCTCCTCTA CCAGCCAGAT ATCTCTTCGC 
AATCTCCCCT CATCCATTCA GTCCCGTCTT TCCATGGTGA ACCAAACAGA CCCCACAGGA 
CAGTCAGTTT TGGTTGGCCT TCCTTCCTCC AGCAGCTCAA GCCAGAGCAT CCCTGCATGC 
AAAAGACACA CCTTAGTGGG CTTTTTAGGA ACAGAAGGAG GCAATAACCT CACTGAAACG 
CAATCCTGTG GCAATTCTAG TCCAGCAACC CATTCAAGAG CCAAAAAGGA AGAGGTTGTT 
TACAGAGTCC AGATTGTGGA TCCAAGCCAA GTGCTTGAAG GCGTGAATAT ATCCAAAAGA 
AAGGAGCTGC AGTGGCCAGA TGAGCTGGTG CGGCTAAAAG CCGGCAAGAA CAGCTGGAAA 
GACTGGAGCC CTCAGGAAGG CATGGAAGGC CATGTAATTC ACCGCTGGGT GCCTTGCAGC 
AGAGACCCTT GTAATAGGTC CCACATTGAC AAAACAATCC TGCTGATTAA GATTGAGGAC 
AAATATGTTG CTGTGATTGA AACTGGTGTG CTTGAACTCG GAGCTGAAGT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pcnx1 ( XM_012969001.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012824455.2 Xenopus tropicalis pecanex 1 (pcnx1), transcript variant X7, mRNA.

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CloneID	OXa64213
Clone ID Related Accession (Same CDS sequence)	XM_012969001.3
Accession Version	XM_012969001.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6639bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pecanex-like protein 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012969001.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCC AGACACTGCA AATCCTCCGG CAGGGAGTCT GGGCTTCCAT CACCGGAGGC 
TGGTACTACG ACCCCCACCA GAGTACCTTT GTCAATACCC TACACCTCTA CATCTGGCTC 
TTCCTCCTCT GCTTCCCCTT CACCCTCTAC ATGGCACTAC CCACATCTTT GGTGATTGTT 
GCTGTGTACT GCCCAGTCGT TGCAGCTGTT TTTATTGTTT TGAAGCTTGT TAACTACCGT 
CTGCACAGGG CATTGGATGA AGGAGAAATT GTGGAGAGGA GTGCTAGTGA GTTGACTGAG 
AGGGTGGCAA AGGCAGAACA CAGTGACAGC TCTGCAAGAA GAGAGGACAG CAATGGACCT 
GGTGATCCAG GTGGAGGGAT TGAAATGTCT GAATTTGTTC GAGAAGCAAC TCCTCCAGTT 
GGATGCAGTT CACGTAATTC CTACTCTGGG TTGGACCCAA GCAATCAGGT TGGTGTGTCT 
CGTTATGGAG GTGCATCTGG AAGCAAAGGG GATACAGATG CTGCAAAGAC ATCAGATGAT 
ATCAGTCTGA GTCTTGGCCA GAGTTACAGT TTGTGCAAAG AAGCAAGTGA AGACCAAGAT 
TTGACTGTGG ATCAGAAGCT TTTCCGTCTC GTCTCCAATG ACTCCTTTGT CTCCATGCAG 
CCATCTATAT CCTCGGGTGG AAAAGACTTA ACTAGAGACT TCAGTGACAA AGGATCAATC 
CCAACACACA GTCATCATCA CCATGTGGAT CTGTCTCTGT CTAATGCTTG TGACACAGAA 
TTGGCCTCAC TTGTTCCATT GCATTCACAT TCGTACAGGA AAGATCATCG ACCAAGAGGT 
GTCCCAAGGA CTTCAAGTTC TGCTGTTGCT TTCCCAGACA CTTCTCTCAC TGACTTTTCA 
CTCTATCAAC CAAGGCGCGG GCTGGATCCT GTTAGTGAAC TTGAAGCTTC CAAACCACAT 
TCTGGGTCCA AAGATTCACT AGTAGATAAT GCTTGTGCTT CAGGGATTTT TCAATTAGAC 
TGTCTAAAAA GCAAAGGAGC CCAGCAGCTT TCAAGAAGTG GTAGTGTTAA ACACATGAAG 
ACTGACAAAA GCATGGATAG TTTAAGAAGC CTCAGCACGA GAAGCAGTGG ATCCACAGAA 
AGTTATTGTA GTGGAACAGA TCGGGACACA AATAGCACTG TTAGCAGCTA CAAAAGTGAA 
CAGACCAGTT CAACTCATGT TGAAAGTATT TTATCTGAGC ATGAGGATGT TACTGCTGCA 
AAGAGAGTCC ATTGTAGTGA CCTGGAGGAA GAACATAGCT CTGCTACTGA TAAAAGGACT 
AGTAGTATAA GAACTGCTTT AGAAAGAAAC ACTGCTCAGG AAGCCAAGGA TGCCAACACA 
TCTGATGATG TACAAAGTCC AAGGGGTCTT AGCTCATCAG CATCAGAGGA AGCTAATAAG 
AATCCACACG CAAATGAGTT AACTGTACAA ACAGACTGGC TTCCAGGTAA ACTGTCAGTG 
AACATTGATG AACCTGGGGA GAAACCAGTC ATTTTAGTTG ACTTGAGATC TTTGGAAGAA 
ACGGGTATTA AGCAGAGAGA CAATGACGTA CGACCCAAAT CATCTAGTTT AATACATCGA 
ACATCCTCTG CTCATAAATC ATGCAGGAGA CGGACAGGTA AAAAAAGAGC CAGCAGTTTT 
GATGCAAGTA GACACAAAGA ATATGTTTCC TTTCAAGGTA TAGCTGATAC CAAACCTCAC 
AGTACTGGCT TTTGTCATGA TGAAGATTCT AGCGACCAAA GCGACTTGAG CAGGACCTCA 
AGCGTTCAGT CGGCCCACCG TTTCAGCAGC GATAGCTCTT CCAGCACTAC TTCCCATTCA 
TGCCAGTCTC CTGAAGCAAG ATACAGTGCC CTAAGGACAA AATATCGAAT AAAAGAAAGA 
GGCACAGATT CACATCACAC ACACCTAAAT TGTGAAGGAA CTAGTAAAAA GCGGTCCAAC 
CGCAGGACTT CTAGTGCAAG TGGTACAAAA AGCCGAGCTA GAGTATTAAG TCTGGACAGT 
GGCACTGTAG TTAGTTTGAG TGATGCCAAC AGACTGGCCG CTCCTGAAAA TATAAAAACC 
TTAACCACTT CAAAATCAGA TCTAGAAGCC AAAGAAGGCG AAGTGCTAGA TCAGATGTCT 
CTGTTGGGGC GAGCTTCACA GTTAGAATCT GTAACACGGT CAAGAAATAG TTTGCCCAGC 
CAGGTTACTT TCCAAGATGT TGGAGAGCGA GACGCATCTG TTGGAGGTAA ATTTACCCCA 
TCTCTATATG AGACTGGAGG TTGTGACATG TCACTTGTAA ACTTTGAGCC AGCTGCAAGA 
AGGACGTCTA ATAATCTTTG TGACACAGAT TCCCATGTAT CAAGTTCTAC CTCAGTTCGA 
TTCTACCCAC ATGATCTAAT CCGATTAAAC AGGCTGCTAA CCATAGATAC AGATCTGTTA 
GAGCAGCAGG ATATTGATCT AAGCCCTGAC CTGCAGGACC AGATGCAACC ACAGGAACAG 
GCTGCCCAAA AAGTAAAACA CTACTACCGG TTTTGGATTC TCCCAAAGCT GTGGGTCGGC 
ATCAACTTTG ACAGGCTTAC ACTCTTAGCC TTGTTCGATA GGAACCGCGA GGTCCTAGAA 
AATGTGCTGT CTGTCATTCT CGCCATTCTA GTTGCCTTTT TGGGCTCTGT TCTTCTCATA 
GAAGGCTTCT TTAAAGATAT CTGGGTCTTC CAGTTTTGTC TTGTTATAGC CAGCTGTCAA 
TACTCTCTCC TTAAGAGTGT TCAACCAGAT TCTTCTTCCC CAAGACATGG CCATAATCGT 
ATCATTGCCT ATAGTAGGCC CGTTTACTTC TGTGCCTGTT GCGGCCTTAT TTGGTTATTG 
GATTATGGAA GCAAAAACAT GTCTACCACC AAATACAAAC TATATGGAAT GGCCTTCACC 
AATCCTCTGC TTCTAGTGTC AGCCAGGGAC TTAGTCATTG TCTTCACACT CTGTTTCCCA 
GTCGTGTTCT TTATTGGTCT CCTGCCTCAG GTGAACACAT TCGTCATGTA CCTGTGTGAG 
CAAGTAGATA TACATGTGTT TGGTGGAAAT GCTACCACCA GCTTGCTTGC AGCAATTTAC 
AGCTTTATCC GTAGCATTGT GGCCATAGCT TTACTCTACG GGCTGTGTTA CGGTGCTCTT 
AAGGATCCAT GGGATGCACA ACACATTCCT GTATTATTCT CTATTTTCTG TGGTGTTCTT 
GTGGCAGTTT CATACCATCT CAGCCGACAG AGCAGCGATC CCTCTGTCCT CATCTCACTG 
GTGCAATCAA AGATTTTTCC AAACAGCGAG GATACAAATC CTGAAGACCC ACTATCAGAA 
GTGAAAGACC CTCTTCCAGA AAAACTGAGA AGCTCTGTTA ATGAACGACT TCAGTCAGAC 
GTAGTAGTCT GTGTCGTTAT TGCAGTGTTG TACTTTGCAA TTCATGTCAG CACCGTATTT 
ACTGCATTAC AGCCGGTTCT GAGCTACGTA CTCTATGCAC TAGTTGGTTC TGTTGGACTC 
CTTACTCACT ACATTCTACC ACAAGTACGG AAACAGCTTC CTTGGCACTG CTTCTCCCAC 
CCGCTGCTGA AAACTAAGGA ATATTACCAA TTTGAAGTTC GAAATGCTGC TCATCTGATG 
TGGTTTGAGA AGGCTCACAT CTGGCTGCTT TTTATCGAGA AGAACATTAT TTATCCTCTT 
ATAGTTCTCA ATGAGCTTAG CATTGGTGCT AAGGATATCG TCAGCCCGAA AAAGCTGGAT 
GCAGAAGTTG GTGCATTGTT GATCACCATT GCCGGAATGA AGCTGCTGAG GTCGTCATTT 
AGCAGTCCCA CCTGCCAGTA TGCCACTGTC ATTTTCACTG TCCTCTTCTT TACTTTTGAC 
TACAAGGAGT ATACAGAAAA ACTGCTGCTA GATCTGTTCT TTATGTCAAT CCTCTTTAGC 
AAGCTCTGGG AACTGTTTTT CAAACTGCGC TTTGTCTACA CCTATATTGC CCCTTGGCAG 
ATCACGTGGG GATCAGCATT CCATGCTTTC GCACAGCCAT TTGCTGTACC TCATTCTGCA 
ATGCTGTTTG TACAGGCAAT TGTTTCTGCA CTGTTCTCCA CTCCCCTGAA CCCTTTCTTA 
GGGAGTGCAA TCTTTATTAC ATCATATGTA CGCCCTGTCA AGTTTTGGGA GAGAGACTAC 
AATACAAAGA GAGTTGACCA CTCAAACACA AGATTAGCCT CTCAGCTTGA CAGAAATCCA 
GGTTCAGATG ACAACAACTT GAATTCCATA TTTTATGAGC ATCTCACACG CTCCCTTCAG 
CATAGTCTGT GTGGTGATCT GTTGCTTGGC CGCTGGGGAA ATTACAATAC TGGGGATTGC 
TTTATTCTGG CCTCAGATTA TCTAAACGCC TTGGTGCACC TTATCGAAAT AGGCAATGGT 
CTGGTCACCT TCCAGCTAAG AGGACTAGAG TTCAGAGGTA CCTACTGCCA GCAGCGTGAA 
GTTGAAGCAA TCACTGAGGG TGTAGAGGAA GATGAAGGCT TTTGCTGTTG CGAACCTGGA 
CATATTCCTC ATATGCTGTC TTTCAATGCT GCCTTTGGGC AGCGCTGGCT GGCATGGGAA 
GTCTTGGTTA CAAAGTATGT TCTGGAAGGT TACAGCATCA CAGACAACAG TGCAGCCTCT 
ATGCTTCAAG TGTTTGACCT TCGCAGGATC CTCACTACCT ATTATGTTAA GGGCATTATA 
TACTATGTGA CAGCATCTGC AAAACTGGAG GAGTGGTTAG CAAATGAGAC AATGCAGGAA 
GGTCTTCGCC AGTGTTCTGA TCATTGTTAT GTTGATGTAG ATCCAACTTT CAACCCAAAT 
ATTGATGAAG ACTATGACCT TCGCTTAGCA GGCATTTCAC GGGAGAGCTT CTGTGCAAGC 
TATCTTAACT GGATTAAGTA CTGTTCCTCT AGAAGGGAGA AGCCTCTTGA TGCTGAAAAG 
GATTCACCTT TGGTAACACT TTGTTATGGG CTCTGTATTT TGGGGAGAAG GGCACTTGGT 
ACAGCTTCTC ATCACATGTC CAGCAATTTG GAGTCCTTTC TATATGGTTT ACATGCCTTG 
TTTAAAGGAG ATTTCCGCAT ATCATCTCTC CGTGATGAGT GGATTTTTGC TGATATGGAG 
CTTTTAAGAA AAGTAGTAGT TCCTGGCATA CGTATGTCAC TAAAACTTCA TCAGGACCAC 
TTTACATCTC CAGATGAGTA TGATGATCCT GCAGTTTTGT ATGAAGCCAT TACATCACAT 
GAGCAGAACC TTGTTATTGC GCATGAGGGA GATCCAGCGT GGAGGAGTGC TGTTCTTTCT 
AACTCACCTT CTCTTCTCGC GCTGCGTCAT GTGATGGATG ATGGAACCAA CGAATACAAG 
ATCATAATGT TAAATAAACG TTATCTTAGT TTTAGAGTCA TTAAGGTAAA CAAGGAGTGT 
GTGCGGGGCT TATGGGCTGG TCAGCAGCAA GAATTGGTCT TTCTTCGTAA TCGGAACCCT 
GAAAGAGGCA GCATCCAAAA TGCAAAGCAG GCTCTCAGGA ATATGATCAA TTCATCATGT 
GACCAACCTA TTGGATACCC AATTTATGTT TCTCCACTGA CAACCTCTTA TTCAGACAGC 
CATGATCAGC TTAAACAGAT ATTTGGTGGG CCAATTAGCT TGGCCAATAT CCGGAACTTC 
ATTGTATCTA CATGGCACAG ATTAAGAAAA GGTTGCGGTG CAGGATGCAA TAGTGGTGGA 
AATATTGAGG ACTCTGACAC TGGAGGCGGG ACCTCCATTA ATAGCAACAA TCCACCAAAT 
GCAAATGAAA CTCAGAGCAG TCTGTCACAA GTTGGTGTCC CAGGTGGAAC AGGGCATGTG 
CCGAATGCAC GGCTCCAGCC TCCGGGGATG CCTCATCCAT CAACACTAGG AACCAGTCAC 
AGTTCTCACT CCATGCAGTC AAGCCTTGTC AGACATTCCC CAGCTCGTGG ATCAGTAGCA 
AGTCAATCAT CTTACTGCTA CAACAGCCGT CACTCATCAC TTAGAACATC CACAACAGGA 
TTTGTGCCTT GTAGGCGCTC CTCTACCAGC CAGATATCTC TTCGCAATCT CCCCTCATCC 
ATTCAGTCCC GTCTTTCCAT GGTGAACCAA ACAGACCCCA CAGGACAGTC AGTTTTGGTT 
GGCCTTCCTT CCTCCAGCAG CTCAAGCCAG AGCATCCCTG CATGCAAAAG ACACACCTTA 
GTGGGCTTTT TAGGAACAGA AGGAGGCAAT AACCTCACTG AAACGCAATC CTGTGGCAAT 
TCTAGTCCAG CAACCCATTC AAGAGCCAAA AAGGAAGAGG TTGTTTACAG AGTCCAGATT 
GTGGATCCAA GCCAAGTGCT TGAAGGCGTG AATATATCCA AAAGAAAGGA GCTGCAGTGG 
CCAGATGAGC TGGTGCGGCT AAAAGCCGGC AAGAACAGCT GGAAAGACTG GAGCCCTCAG 
GAAGGCATGG AAGGCCATGT AATTCACCGC TGGGTGCCTT GCAGCAGAGA CCCTTGTAAT 
AGGTCCCACA TTGACAAAAC AATCCTGCTG ATTAAGATTG AGGACAAATA TGTTGCTGTG 
ATTGAAACTG GTGTGCTTGA ACTCGGAGCT GAAGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012824455.2
CDS	276..6914
Translation	MGSQTLQILRQGVWASITGGWYYDPHQSTFVNTLHLYIWLFLLCFPFTLYMALPTSLVIVAVYCPVVAAVFIVLKLVNYRLHRALDEGEIVERSASELTERVAKAEHSDSSARREDSNGPGDPGGGIEMSEFVREATPPVGCSSRNSYSGLDPSNQVGVSRYGGASGSKGDTDAAKTSDDISLSLGQSYSLCKEASEDQDLTVDQKLFRLVSNDSFVSMQPSISSGGKDLTRDFSDKGSIPTHSHHHHVDLSLSNACDTELASLVPLHSHSYRKDHRPRGVPRTSSSAVAFPDTSLTDFSLYQPRRGLDPVSELEASKPHSGSKDSLVDNACASGIFQLDCLKSKGAQQLSRSGSVKHMKTDKSMDSLRSLSTRSSGSTESYCSGTDRDTNSTVSSYKSEQTSSTHVESILSEHEDVTAAKRVHCSDLEEEHSSATDKRTSSIRTALERNTAQEAKDANTSDDVQSPRGLSSSASEEANKNPHANELTVQTDWLPGKLSVNIDEPGEKPVILVDLRSLEETGIKQRDNDVRPKSSSLIHRTSSAHKSCRRRTGKKRASSFDASRHKEYVSFQGIADTKPHSTGFCHDEDSSDQSDLSRTSSVQSAHRFSSDSSSSTTSHSCQSPEARYSALRTKYRIKERGTDSHHTHLNCEGTSKKRSNRRTSSASGTKSRARVLSLDSGTVVSLSDANRLAAPENIKTLTTSKSDLEAKEGEVLDQMSLLGRASQLESVTRSRNSLPSQVTFQDVGERDASVGGKFTPSLYETGGCDMSLVNFEPAARRTSNNLCDTDSHVSSSTSVRFYPHDLIRLNRLLTIDTDLLEQQDIDLSPDLQDQMQPQEQAAQKVKHYYRFWILPKLWVGINFDRLTLLALFDRNREVLENVLSVILAILVAFLGSVLLIEGFFKDIWVFQFCLVIASCQYSLLKSVQPDSSSPRHGHNRIIAYSRPVYFCACCGLIWLLDYGSKNMSTTKYKLYGMAFTNPLLLVSARDLVIVFTLCFPVVFFIGLLPQVNTFVMYLCEQVDIHVFGGNATTSLLAAIYSFIRSIVAIALLYGLCYGALKDPWDAQHIPVLFSIFCGVLVAVSYHLSRQSSDPSVLISLVQSKIFPNSEDTNPEDPLSEVKDPLPEKLRSSVNERLQSDVVVCVVIAVLYFAIHVSTVFTALQPVLSYVLYALVGSVGLLTHYILPQVRKQLPWHCFSHPLLKTKEYYQFEVRNAAHLMWFEKAHIWLLFIEKNIIYPLIVLNELSIGAKDIVSPKKLDAEVGALLITIAGMKLLRSSFSSPTCQYATVIFTVLFFTFDYKEYTEKLLLDLFFMSILFSKLWELFFKLRFVYTYIAPWQITWGSAFHAFAQPFAVPHSAMLFVQAIVSALFSTPLNPFLGSAIFITSYVRPVKFWERDYNTKRVDHSNTRLASQLDRNPGSDDNNLNSIFYEHLTRSLQHSLCGDLLLGRWGNYNTGDCFILASDYLNALVHLIEIGNGLVTFQLRGLEFRGTYCQQREVEAITEGVEEDEGFCCCEPGHIPHMLSFNAAFGQRWLAWEVLVTKYVLEGYSITDNSAASMLQVFDLRRILTTYYVKGIIYYVTASAKLEEWLANETMQEGLRQCSDHCYVDVDPTFNPNIDEDYDLRLAGISRESFCASYLNWIKYCSSRREKPLDAEKDSPLVTLCYGLCILGRRALGTASHHMSSNLESFLYGLHALFKGDFRISSLRDEWIFADMELLRKVVVPGIRMSLKLHQDHFTSPDEYDDPAVLYEAITSHEQNLVIAHEGDPAWRSAVLSNSPSLLALRHVMDDGTNEYKIIMLNKRYLSFRVIKVNKECVRGLWAGQQQELVFLRNRNPERGSIQNAKQALRNMINSSCDQPIGYPIYVSPLTTSYSDSHDQLKQIFGGPISLANIRNFIVSTWHRLRKGCGAGCNSGGNIEDSDTGGGTSINSNNPPNANETQSSLSQVGVPGGTGHVPNARLQPPGMPHPSTLGTSHSSHSMQSSLVRHSPARGSVASQSSYCYNSRHSSLRTSTTGFVPCRRSSTSQISLRNLPSSIQSRLSMVNQTDPTGQSVLVGLPSSSSSSQSIPACKRHTLVGFLGTEGGNNLTETQSCGNSSPATHSRAKKEEVVYRVQIVDPSQVLEGVNISKRKELQWPDELVRLKAGKNSWKDWSPQEGMEGHVIHRWVPCSRDPCNRSHIDKTILLIKIEDKYVAVIETGVLELGAEV

Target ORF information:

RefSeq Version	XM_012969001.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pecanex 1 (pcnx1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012969001.3

ORF Insert Sequence:

ATGGGTTCCC AGACACTGCA AATCCTCCGG CAGGGAGTCT GGGCTTCCAT CACCGGAGGC 
TGGTACTACG ACCCCCACCA GAGTACCTTT GTCAATACCC TACACCTCTA CATCTGGCTC 
TTCCTCCTCT GCTTCCCCTT CACCCTCTAC ATGGCACTAC CCACATCTTT GGTGATTGTT 
GCTGTGTACT GCCCAGTCGT TGCAGCTGTT TTTATTGTTT TGAAGCTTGT TAACTACCGT 
CTGCACAGGG CATTGGATGA AGGAGAAATT GTGGAGAGGA GTGCTAGTGA GTTGACTGAG 
AGGGTGGCAA AGGCAGAACA CAGTGACAGC TCTGCAAGAA GAGAGGACAG CAATGGACCT 
GGTGATCCAG GTGGAGGGAT TGAAATGTCT GAATTTGTTC GAGAAGCAAC TCCTCCAGTT 
GGATGCAGTT CACGTAATTC CTACTCTGGG TTGGACCCAA GCAATCAGGT TGGTGTGTCT 
CGTTATGGAG GTGCATCTGG AAGCAAAGGG GATACAGATG CTGCAAAGAC ATCAGATGAT 
ATCAGTCTGA GTCTTGGCCA GAGTTACAGT TTGTGCAAAG AAGCAAGTGA AGACCAAGAT 
TTGACTGTGG ATCAGAAGCT TTTCCGTCTC GTCTCCAATG ACTCCTTTGT CTCCATGCAG 
CCATCTATAT CCTCGGGTGG AAAAGACTTA ACTAGAGACT TCAGTGACAA AGGATCAATC 
CCAACACACA GTCATCATCA CCATGTGGAT CTGTCTCTGT CTAATGCTTG TGACACAGAA 
TTGGCCTCAC TTGTTCCATT GCATTCACAT TCGTACAGGA AAGATCATCG ACCAAGAGGT 
GTCCCAAGGA CTTCAAGTTC TGCTGTTGCT TTCCCAGACA CTTCTCTCAC TGACTTTTCA 
CTCTATCAAC CAAGGCGCGG GCTGGATCCT GTTAGTGAAC TTGAAGCTTC CAAACCACAT 
TCTGGGTCCA AAGATTCACT AGTAGATAAT GCTTGTGCTT CAGGGATTTT TCAATTAGAC 
TGTCTAAAAA GCAAAGGAGC CCAGCAGCTT TCAAGAAGTG GTAGTGTTAA ACACATGAAG 
ACTGACAAAA GCATGGATAG TTTAAGAAGC CTCAGCACGA GAAGCAGTGG ATCCACAGAA 
AGTTATTGTA GTGGAACAGA TCGGGACACA AATAGCACTG TTAGCAGCTA CAAAAGTGAA 
CAGACCAGTT CAACTCATGT TGAAAGTATT TTATCTGAGC ATGAGGATGT TACTGCTGCA 
AAGAGAGTCC ATTGTAGTGA CCTGGAGGAA GAACATAGCT CTGCTACTGA TAAAAGGACT 
AGTAGTATAA GAACTGCTTT AGAAAGAAAC ACTGCTCAGG AAGCCAAGGA TGCCAACACA 
TCTGATGATG TACAAAGTCC AAGGGGTCTT AGCTCATCAG CATCAGAGGA AGCTAATAAG 
AATCCACACG CAAATGAGTT AACTGTACAA ACAGACTGGC TTCCAGGTAA ACTGTCAGTG 
AACATTGATG AACCTGGGGA GAAACCAGTC ATTTTAGTTG ACTTGAGATC TTTGGAAGAA 
ACGGGTATTA AGCAGAGAGA CAATGACGTA CGACCCAAAT CATCTAGTTT AATACATCGA 
ACATCCTCTG CTCATAAATC ATGCAGGAGA CGGACAGGTA AAAAAAGAGC CAGCAGTTTT 
GATGCAAGTA GACACAAAGA ATATGTTTCC TTTCAAGGTA TAGCTGATAC CAAACCTCAC 
AGTACTGGCT TTTGTCATGA TGAAGATTCT AGCGACCAAA GCGACTTGAG CAGGACCTCA 
AGCGTTCAGT CGGCCCACCG TTTCAGCAGC GATAGCTCTT CCAGCACTAC TTCCCATTCA 
TGCCAGTCTC CTGAAGCAAG ATACAGTGCC CTAAGGACAA AATATCGAAT AAAAGAAAGA 
GGCACAGATT CACATCACAC ACACCTAAAT TGTGAAGGAA CTAGTAAAAA GCGGTCCAAC 
CGCAGGACTT CTAGTGCAAG TGGTACAAAA AGCCGAGCTA GAGTATTAAG TCTGGACAGT 
GGCACTGTAG TTAGTTTGAG TGATGCCAAC AGACTGGCCG CTCCTGAAAA TATAAAAACC 
TTAACCACTT CAAAATCAGA TCTAGAAGCC AAAGAAGGCG AAGTGCTAGA TCAGATGTCT 
CTGTTGGGGC GAGCTTCACA GTTAGAATCT GTAACACGGT CAAGAAATAG TTTGCCCAGC 
CAGGTTACTT TCCAAGATGT TGGAGAGCGA GACGCATCTG TTGGAGGTAA ATTTACCCCA 
TCTCTATATG AGACTGGAGG TTGTGACATG TCACTTGTAA ACTTTGAGCC AGCTGCAAGA 
AGGACGTCTA ATAATCTTTG TGACACAGAT TCCCATGTAT CAAGTTCTAC CTCAGTTCGA 
TTCTACCCAC ATGATCTAAT CCGATTAAAC AGGCTGCTAA CCATAGATAC AGATCTGTTA 
GAGCAGCAGG ATATTGATCT AAGCCCTGAC CTGCAGGACC AGATGCAACC ACAGGAACAG 
GCTGCCCAAA AAGTAAAACA CTACTACCGG TTTTGGATTC TCCCAAAGCT GTGGGTCGGC 
ATCAACTTTG ACAGGCTTAC ACTCTTAGCC TTGTTCGATA GGAACCGCGA GGTCCTAGAA 
AATGTGCTGT CTGTCATTCT CGCCATTCTA GTTGCCTTTT TGGGCTCTGT TCTTCTCATA 
GAAGGCTTCT TTAAAGATAT CTGGGTCTTC CAGTTTTGTC TTGTTATAGC CAGCTGTCAA 
TACTCTCTCC TTAAGAGTGT TCAACCAGAT TCTTCTTCCC CAAGACATGG CCATAATCGT 
ATCATTGCCT ATAGTAGGCC CGTTTACTTC TGTGCCTGTT GCGGCCTTAT TTGGTTATTG 
GATTATGGAA GCAAAAACAT GTCTACCACC AAATACAAAC TATATGGAAT GGCCTTCACC 
AATCCTCTGC TTCTAGTGTC AGCCAGGGAC TTAGTCATTG TCTTCACACT CTGTTTCCCA 
GTCGTGTTCT TTATTGGTCT CCTGCCTCAG GTGAACACAT TCGTCATGTA CCTGTGTGAG 
CAAGTAGATA TACATGTGTT TGGTGGAAAT GCTACCACCA GCTTGCTTGC AGCAATTTAC 
AGCTTTATCC GTAGCATTGT GGCCATAGCT TTACTCTACG GGCTGTGTTA CGGTGCTCTT 
AAGGATCCAT GGGATGCACA ACACATTCCT GTATTATTCT CTATTTTCTG TGGTGTTCTT 
GTGGCAGTTT CATACCATCT CAGCCGACAG AGCAGCGATC CCTCTGTCCT CATCTCACTG 
GTGCAATCAA AGATTTTTCC AAACAGCGAG GATACAAATC CTGAAGACCC ACTATCAGAA 
GTGAAAGACC CTCTTCCAGA AAAACTGAGA AGCTCTGTTA ATGAACGACT TCAGTCAGAC 
GTAGTAGTCT GTGTCGTTAT TGCAGTGTTG TACTTTGCAA TTCATGTCAG CACCGTATTT 
ACTGCATTAC AGCCGGTTCT GAGCTACGTA CTCTATGCAC TAGTTGGTTC TGTTGGACTC 
CTTACTCACT ACATTCTACC ACAAGTACGG AAACAGCTTC CTTGGCACTG CTTCTCCCAC 
CCGCTGCTGA AAACTAAGGA ATATTACCAA TTTGAAGTTC GAAATGCTGC TCATCTGATG 
TGGTTTGAGA AGGCTCACAT CTGGCTGCTT TTTATCGAGA AGAACATTAT TTATCCTCTT 
ATAGTTCTCA ATGAGCTTAG CATTGGTGCT AAGGATATCG TCAGCCCGAA AAAGCTGGAT 
GCAGAAGTTG GTGCATTGTT GATCACCATT GCCGGAATGA AGCTGCTGAG GTCGTCATTT 
AGCAGTCCCA CCTGCCAGTA TGCCACTGTC ATTTTCACTG TCCTCTTCTT TACTTTTGAC 
TACAAGGAGT ATACAGAAAA ACTGCTGCTA GATCTGTTCT TTATGTCAAT CCTCTTTAGC 
AAGCTCTGGG AACTGTTTTT CAAACTGCGC TTTGTCTACA CCTATATTGC CCCTTGGCAG 
ATCACGTGGG GATCAGCATT CCATGCTTTC GCACAGCCAT TTGCTGTACC TCATTCTGCA 
ATGCTGTTTG TACAGGCAAT TGTTTCTGCA CTGTTCTCCA CTCCCCTGAA CCCTTTCTTA 
GGGAGTGCAA TCTTTATTAC ATCATATGTA CGCCCTGTCA AGTTTTGGGA GAGAGACTAC 
AATACAAAGA GAGTTGACCA CTCAAACACA AGATTAGCCT CTCAGCTTGA CAGAAATCCA 
GGTTCAGATG ACAACAACTT GAATTCCATA TTTTATGAGC ATCTCACACG CTCCCTTCAG 
CATAGTCTGT GTGGTGATCT GTTGCTTGGC CGCTGGGGAA ATTACAATAC TGGGGATTGC 
TTTATTCTGG CCTCAGATTA TCTAAACGCC TTGGTGCACC TTATCGAAAT AGGCAATGGT 
CTGGTCACCT TCCAGCTAAG AGGACTAGAG TTCAGAGGTA CCTACTGCCA GCAGCGTGAA 
GTTGAAGCAA TCACTGAGGG TGTAGAGGAA GATGAAGGCT TTTGCTGTTG CGAACCTGGA 
CATATTCCTC ATATGCTGTC TTTCAATGCT GCCTTTGGGC AGCGCTGGCT GGCATGGGAA 
GTCTTGGTTA CAAAGTATGT TCTGGAAGGT TACAGCATCA CAGACAACAG TGCAGCCTCT 
ATGCTTCAAG TGTTTGACCT TCGCAGGATC CTCACTACCT ATTATGTTAA GGGCATTATA 
TACTATGTGA CAGCATCTGC AAAACTGGAG GAGTGGTTAG CAAATGAGAC AATGCAGGAA 
GGTCTTCGCC AGTGTTCTGA TCATTGTTAT GTTGATGTAG ATCCAACTTT CAACCCAAAT 
ATTGATGAAG ACTATGACCT TCGCTTAGCA GGCATTTCAC GGGAGAGCTT CTGTGCAAGC 
TATCTTAACT GGATTAAGTA CTGTTCCTCT AGAAGGGAGA AGCCTCTTGA TGCTGAAAAG 
GATTCACCTT TGGTAACACT TTGTTATGGG CTCTGTATTT TGGGGAGAAG GGCACTTGGT 
ACAGCTTCTC ATCACATGTC CAGCAATTTG GAGTCCTTTC TATATGGTTT ACATGCCTTG 
TTTAAAGGAG ATTTCCGCAT ATCATCTCTC CGTGATGAGT GGATTTTTGC TGATATGGAG 
CTTTTAAGAA AAGTAGTAGT TCCTGGCATA CGTATGTCAC TAAAACTTCA TCAGGACCAC 
TTTACATCTC CAGATGAGTA TGATGATCCT GCAGTTTTGT ATGAAGCCAT TACATCACAT 
GAGCAGAACC TTGTTATTGC GCATGAGGGA GATCCAGCGT GGAGGAGTGC TGTTCTTTCT 
AACTCACCTT CTCTTCTCGC GCTGCGTCAT GTGATGGATG ATGGAACCAA CGAATACAAG 
ATCATAATGT TAAATAAACG TTATCTTAGT TTTAGAGTCA TTAAGGTAAA CAAGGAGTGT 
GTGCGGGGCT TATGGGCTGG TCAGCAGCAA GAATTGGTCT TTCTTCGTAA TCGGAACCCT 
GAAAGAGGCA GCATCCAAAA TGCAAAGCAG GCTCTCAGGA ATATGATCAA TTCATCATGT 
GACCAACCTA TTGGATACCC AATTTATGTT TCTCCACTGA CAACCTCTTA TTCAGACAGC 
CATGATCAGC TTAAACAGAT ATTTGGTGGG CCAATTAGCT TGGCCAATAT CCGGAACTTC 
ATTGTATCTA CATGGCACAG ATTAAGAAAA GGTTGCGGTG CAGGATGCAA TAGTGGTGGA 
AATATTGAGG ACTCTGACAC TGGAGGCGGG ACCTCCATTA ATAGCAACAA TCCACCAAAT 
GCAAATGAAA CTCAGAGCAG TCTGTCACAA GTTGGTGTCC CAGGTGGAAC AGGGCATGTG 
CCGAATGCAC GGCTCCAGCC TCCGGGGATG CCTCATCCAT CAACACTAGG AACCAGTCAC 
AGTTCTCACT CCATGCAGTC AAGCCTTGTC AGACATTCCC CAGCTCGTGG ATCAGTAGCA 
AGTCAATCAT CTTACTGCTA CAACAGCCGT CACTCATCAC TTAGAACATC CACAACAGGA 
TTTGTGCCTT GTAGGCGCTC CTCTACCAGC CAGATATCTC TTCGCAATCT CCCCTCATCC 
ATTCAGTCCC GTCTTTCCAT GGTGAACCAA ACAGACCCCA CAGGACAGTC AGTTTTGGTT 
GGCCTTCCTT CCTCCAGCAG CTCAAGCCAG AGCATCCCTG CATGCAAAAG ACACACCTTA 
GTGGGCTTTT TAGGAACAGA AGGAGGCAAT AACCTCACTG AAACGCAATC CTGTGGCAAT 
TCTAGTCCAG CAACCCATTC AAGAGCCAAA AAGGAAGAGG TTGTTTACAG AGTCCAGATT 
GTGGATCCAA GCCAAGTGCT TGAAGGCGTG AATATATCCA AAAGAAAGGA GCTGCAGTGG 
CCAGATGAGC TGGTGCGGCT AAAAGCCGGC AAGAACAGCT GGAAAGACTG GAGCCCTCAG 
GAAGGCATGG AAGGCCATGT AATTCACCGC TGGGTGCCTT GCAGCAGAGA CCCTTGTAAT 
AGGTCCCACA TTGACAAAAC AATCCTGCTG ATTAAGATTG AGGACAAATA TGTTGCTGTG 
ATTGAAACTG GTGTGCTTGA ACTCGGAGCT GAAGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pcnx1 ( XM_004917251.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004917308.1 Xenopus tropicalis pecanex homolog (pcnx), transcript variant X1, mRNA.

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CloneID	OXa15003
Clone ID Related Accession (Same CDS sequence)	XM_004917251.3
Accession Version	XM_004917251.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7026bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pecanex-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004917251.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCC AGACACTGCA AATCCTCCGG CAGGGAGTCT GGGCTTCCAT CACCGGAGGC 
TGGTACTACG ACCCCCACCA GAGTACCTTT GTCAATACCC TACACCTCTA CATCTGGCTC 
TTCCTCCTCT GCTTCCCCTT CACCCTCTAC ATGGCACTAC CCACATCTTT GGTGATTGTT 
GCTGTGTACT GCCCAGTCGT TGCAGCTGTT TTTATTGTTT TGAAGCTTGT TAACTACCGT 
CTGCACAGGG CATTGGATGA AGGAGAAATT GTGGAGAGGA GTGCTAGTGA GTTGACTGAG 
AGGGTGGCAA AGGCAGAACA CAGTGACAGC TCTGCAAGAA GAGAGGACAG CAATGGACCT 
GGTGATCCAG GTGGAGGGAT TGAAATGTCT GAATTTGTTC GAGAAGCAAC TCCTCCAGTT 
GGATGCAGTT CACGTAATTC CTACTCTGGG TTGGACCCAA GCAATCAGGT TGGTGTGTCT 
CGTTATGGAG GTGCATCTGG AAGCAAAGGG GATACAGATG CTGCAAAGAC ATCAGATGAT 
ATCAGTCTGA GTCTTGGCCA GAGTTACAGT TTGTGCAAAG AAGCAAGTGA AGACCAAGAT 
TTGACTGTGG ATCAGAAGCT TTTCCGTCTC GTCTCCAATG ACTCCTTTGT CTCCATGCAG 
CCATCTATAT CCTCGGGTGG AAAAGACTTA ACTAGAGACT TCAGTGACAA AGGATCAATC 
CCAACACACA GTCATCATCA CCATGTGGAT CTGTCTCTGT CTAATGCTTG TGACACAGAA 
TTGGCCTCAC TTGTTCCATT GCATTCACAT TCGTACAGGA AAGATCATCG ACCAAGAGGT 
GTCCCAAGGA CTTCAAGTTC TGCTGTTGCT TTCCCAGACA CTTCTCTCAC TGACTTTTCA 
CTCTATCAAC CAAGGCGCGG GCTGGATCCT GTTAGTGAAC TTGAAGCTTC CAAACCACAT 
TCTGGGTCCA AAGATTCACT AGTACATAAT GCTTGTGCTT CAGGGATTTT TCAATTAGAC 
TGTCTAAAAA GCAAAGGAGC CCAGCAGCTT TCAAGAAGTG GTAGTGTTAA ACACATGAAG 
ACTGACAAAA GCATGGATAG TTTAAGAAGC CTCAGCACGA GAAGCAGTGG ATCCACAGAA 
AGTTATTGTA GTGGAACAGA TCGGGACACA AATAGCACTG TTAGCAGCTA CAAAAGTGAA 
CAGACCAGTT CAACTCATGT TGAAAGTATT TTATCTGAGC ATGAGGATGT TACTGCTGCA 
AAGAGAGTCC ATTGTAGTGA CCTGGAGGAA GAACATAGCT CTGCTACTGA TAAAAGGACT 
AGTAGTATAA GAACTGCTTT AGAAAGAAAC ACTGCTCAGG AAGCCAAGGA TGCCAACACA 
TCTGATGATG TACAAAGTCC AAGGGGTCTT AGCTCATCAG CATCAGAGGA AGCTAATAAG 
AATCCACACG CAAATGAGTT AACTGTACAA ACAGACTGGC TTCCAGGTAA ACTGTCAGTG 
AACATTGATG AACCTGGGGA GAAACCAGTC ATTTTAGTTG ACTTGAGATC TTTGAAAGAA 
ACGGGTATTA AGCAGAGAGA CAATGACGTA CGACCCAAAT CATCTAGTTT AATACATCGA 
ACATCCTCTG CTCATAAATC ATGCAGGAGA CGGACAGGTA AAAAAAGAGC CAGCAGTTTT 
GATGCAAGTA GACACAAAGA ATATGTTTCC TTTCAAGGTA TAGCTGATAC CAAACCTCAC 
AGTACTGGCT TTTGTCATGA TGAAGATTCT AGCGACCAAA GCGACTTGAG CAGGACCTCA 
AGCGTTCAGT CGGCCCACCG TTTCAGCAGC GATAGCTCTT CCAGCACTAC TTCCCATTCA 
TGCCAGTCTC CTGAAGCAAG ATACAGTGCC CTAAGGACAA AATATCGAAT AAAAGAAAGA 
GGCACAGATT CACATCACAC ACACCTAAAT TGTGAAGGAA CTAGTAAAAA GCGGTCCAAC 
CGCAGGACTT CTAGTGCAAG TGGTACAAAA AGCCGAGCTA GAGTATTAAG TCTGGACAGT 
GGCACTGTAG TTAGTTTGAG TGATGCCAAC AGACTGGCCG CTCCTGAAAA TATAAAAACC 
TTAACCACTT CAAAATCAGA TCTAGAAGCC AAAGAAGGCG AAGTGCTAGA TCAGATGTCT 
CTGTTGGGGC GAGCTTCACA GTTAGAATCT GTAACACGGT CAAGAAATAG TTTGCCCAGC 
CAGGTTACTT TCCAAGATGT TGGAGAGCGA GACGCATCTG TTGGAGCACA CGCCAGTGAG 
GAGACAGTAA CATTTCGCCG CGAACGCAGC ACATTTAGGC GCCAAGCGGT TCGGCGCAGA 
CACAATGCAG GGAGTAATCC CACCCCTCCT GCGTCACTCA TCGGATCACC TCTGAGCCTT 
CAAGATGGTC ATCAAGGCCA GCAGTCATCA ACCCAGGTCA AAGTACAGTC CCGCCCCCCT 
TCCCAGGCTG CAGTGCTCAG CGCTAGCGCC TCCTTGCTGG TGAGAAATGG GAGTGTCCAC 
TTAGAAGCGT CTCATGACAA TGCATCTGCC GTAGGCAGTA GCAGTTTGCA CGATGAACTT 
GGTATGCAGG CCTTATGTAA CCTTGGTGAC ATGGAAGTTT TAATGCAGCA CATTTTGGGT 
AAATTTACCC CATCTCTATA TGAGACTGGA GGTTGTGACA TGTCACTTGT AAACTTTGAG 
CCAGCTGCAA GAAGGACGTC TAATAATCTT TGTGACACAG ATTCCCATGT ATCAAGTTCT 
ACCTCAGTTC GGTTCTACCC ACATGATCTA CTCTCTCTTC CCCAGATCCG ATTAAACAGG 
CTGCTAACCA TAGATACAGA TCTGTTAGAG CAGCAGGATA TTGATCTAAG CCCTGACCTG 
CAGGACCAGA TGCAACCACA GGAACAGGCT GCCCAAAAAG TAAAACACTA CTACCGGTTT 
TGGATTCTCC CAAAGCTGTG GGTCGGCATC AACTTTGACA GGCTTACACT CTTAGCCTTG 
TTCGATAGGA ACCGCGAGGT CCTAGAAAAT GTGTTGTCTG TCATTCTCGC CATTCTAGTT 
GCCTTTTTGG GCTCTGTTCT TCTCATAGAA GGCTTCTTTA AAGATATCTG GGTCTTCCAG 
TTTTGTCTTG TTATAGCCAG CTGTCAATAC TCTCTCCTTA AGAGTGTTCA ACCAGATTCT 
TCTTCCCCAA GACATGGCCA TAATCGTATC ATTGCCTATA GTAGGCCCGT TTACTTCTGT 
GCCTGTTGCG GCCTTATTTG GTTATTGGAT TATGGAAGCA AAAACATGTC TACCACCAAA 
TACAAACTAT ATGGAATGGC CTTCACCAAT CCTCTGCTTC TAGTGTCAGC CAGGGACTTA 
GTCATTGTCT TCACACTCTG TTTCCCAGTC GTGTTCTTTA TTGGTCTCCT GCCTCAGGTG 
AACACATTCG TCATGTACCT GTGTGAGCAA GTAGATATAC ATGTGTTTGG TGGAAATGCT 
ACCACCAGCT TGCTTGCAGC AATTTACAGC TTTATCCGTA GCATTGTGGC CATAGCTTTA 
CTCTACGGGC TGTGTTACGG TGCTCTTAAG GATCCATGGG ATGCACAACA CATTCCTGTA 
TTATTCTCTA TTTTCTGTGG TGTTCTTGTG GCAGTTTCAT ACCATCTCAG CCGACAGAGC 
AGCGATCCCT CTGTCCTCAT CTCACTGGTG CAATCAAAGA TTTTTCCAAA CAGCGAGGAT 
ACAAATCCTG AAGACCCACT ATCAGAAGTG AAAGACCCTC TTCCAGAAAA ACTGAGAAGC 
TCTGTTAATG AACGACTTCA GTCAGACGTA GTAGTCTGTG TCGTTATTGC AGTGTTGTAC 
TTTGCAATTC ATGTCAGCAC CGTATTTACT GCATTACAGC CGGTTCTGAG CTACGTACTC 
TATGCACTAG TTGGTTCTGT TGGACTCCTT ACTCACTACA TTCTACCACA AGTACGGAAA 
CAGCTTCCTT GGCACTGCTT CTCCCACCCG CTGCTGAAAA CTAAGGAATA TTACCAATTT 
GAAGTTCGAA ATGCTGCTCA TCTGATGTGG TTTGAGAAGG CTCACATCTG GCTGCTTTTT 
ATCGAGAAGA ACATTATTTA TCCTCTTATA GTTCTCAATG AGCTTAGCAT TGGTGCTAAG 
GATATCGTCA GCCCGAAAAA GCTGGATGCA GAAGTTGGTG CATTGTTGAT CACCATTGCC 
GGAATGAAGC TGCTGAGGTC GTCATTTAGC AGTCCCACCT GCCAGTATGC CACTGTCATT 
TTCACTGTCC TCTTCTTTAC TTTTGACTAC AAGGAGTATA CAGAAAAACT GCTGCTAGAT 
CTGTTCTTTA TGTCAATCCT CTTTAGCAAG CTCTGGGAAC TGTTTTTCAA ACTGCGCTTT 
GTCTACACCT ATATTGCCCC TTGGCAGATC ACGTGGGGAT CAGCATTCCA TGCTTTCGCA 
CAGCCATTTG CTGTACCTCA TTCTGCAATG CTGTTTGTAC AGGCAATTGT TTCTGCACTG 
TTCTCCACTC CCCTGAACCC TTTCTTAGGG AGTGCAATCT TTATTACATC ATATGTACGC 
CCTGTCAAGT TTTGGGAGAG AGACTACAAT ACAAAGAGAG TTGACCACTC AAACACAAGA 
TTAGCCTCTC AGCTTGACAG AAATCCAGGT TCAGATGACA ACAACTTGAA TTCCATATTT 
TATGAGCATC TCACACGCTC CCTTCAGCAT AGTCTGTGTG GTGATCTGTT GCTTGGCCGC 
TGGGGAAATT ACAATACTGG GGATTGCTTT ATTCTGGCCT CAGATTATCT AAACGCCTTG 
GTGCACCTTA TCGAAATAGG CAATGGTCTG GTCACCTTCC AGCTAAGAGG ACTAGAGTTC 
AGAGGTACCT ACTGCCAGCA GCGTGAAGTT GAAGCAATCA CTGAGGGTGT AGAGGAAGAT 
GAAGGCTTTT GCTGTTGCGA ACCTGGACAT ATTCCTCATA TGCTGTCTTT CAATGCTGCC 
TTTGGGCAGC GCTGGCTGGC ATGGGAAGTC TTGGTTACAA AGTATGTTCT GGAAGGTTAC 
AGCATCACAG ACAACAGTGC AGCCTCTATG CTTCAAGTGT TTGACCTTCG CAGGATCCTC 
ACTACCTATT ATGTTAAGGG CATTATATAC TATGTGACAG CATCTGCAAA ACTGGAGGAG 
TGGTTAGCAA ATGAGACAAT GCAGGAAGGT CTTCGCCAGT GTTCTGATCA TTGTTATGTT 
GATGTAGATC CAACTTTCAA CCCAAATATT GATGAAGACT ATGACCTTCG CTTAGCAGGC 
ATTTCACGGG AGAGCTTCTG TGCAAGCTAT CTTAACTGGA TTAAGTACTG TTCCTCTAGA 
AGGGAGAAGC CTCTTGATGC TGAAAAGGAT TCACCTTTGG TAACACTTTG TTATGGGCTC 
TGTATTTTGG GGAGAAGGGC ACTTGGTACA GCTTCTCATC ACATGTCCAG CAATTTGGAG 
TCCTTTCTAT ATGGTTTACA TGCCTTGTTT AAAGGAGATT TCCGCATATC ATCTCTCCGT 
GATGAGTGGA TTTTTGCTGA TATGGAGCTT TTAAGAAAAG TAGTAGTTCC TGGCATACGT 
ATGTCACTAA AACTTCATCA GGACCACTTT ACATCTCCAG ATGAGTATGA TGATCCTGCA 
GTTTTGTATG AAGCCATTAC ATCACATGAG CAGAACCTTG TTATTGCGCA TGAGGGAGAT 
CCAGCGTGGA GGAGTGCTGT TCTTTCTAAC TCACCTTCTC TTCTCGCGCT GCGTCATGTG 
ATGGATGATG GAACCAACGA ATACAAGATC ATAATGTTAA ATAAACGTTA TCTTAGTTTT 
AGAGTCATTA AGGTAAACAA GGAGTGTGTG CGGGGCTTAT GGGCTGGTCA GCAGCAAGAA 
TTGGTCTTTC TTCGTAATCG GAACCCTGAA AGAGGCAGCA TCCAAAATGC AAAGCAGGCT 
CTCAGGAATA TGATCAATTC ATCATGTGAC CAACCTATTG GATACCCAAT TTATGTTTCT 
CCACTGACAA CCTCTTATTC AGACAGCCAT GATCAGCTTA AACAGATATT TGGTGGGCCA 
ATTAGCTTGG CCAATATCCG GAACTTCATT GTATCTACAT GGCACAGATT AAGAAAAGGT 
TGCGGTGCAG GATGCAATAG TGGTGGAAAT ATTGAGGACT CTGACACTGG AGGCGGGACC 
TCCATTAATA GCAACAATCC ACCAAATGCA AATGAAACTC AGAGCAGTCT GTCACAAGTT 
GGTGTCCCAG GTGGAACAGG GCATGTGCCG AATGCACGGC TCCAGCCTCC GGGGATGCCT 
CATCCATCAA CACTAGGAAC CAGTCACAGT TCTCACTCCA TGCAGTCAAG CCTTGTCAGA 
CATTCCCCAG CTCGTGGATC AGTAGCAAGT CAATCATCTT ACTGCTACAA CAGCCGTCAC 
TCATCACTTA GAACATCCAC AACAGGATTT GTGCCTTGTA GGCGCTCCTC TACCAGCCAG 
ATATCTCTTC GCAATCTCCC CTCATCCATT CAGTCCCGTC TTTCCATGGT GAACCAAACA 
GACCCCACAG GACAGTCAGT TTTGGTTGGC CTTCCTTCCT CCAGCAGCTC AAGCCAGAGC 
ATCCCTGCAT GCAAAAGACA CACCTTAGTG GGCTTTTTAG GAACAGAAGG AGGCAATAAC 
CTCACTGAAA CGCAATCCTG TGGCAATTCT AGTCCAGCAA CCCATTCAAG AGCCAAAAAG 
GAAGAGGTTG TTTACAGAGT CCAGATTGTG GATCCAAGCC AAGTGCTTGA AGGCGTGAAT 
ATATCCAAAA GAAAGGAGCT GCAGTGGCCA GATGAGCTGG TGCGGCTAAA AGCCGGCAAG 
AACAGCTGGA AAGACTGGAG CCCTCAGGAA GGCATGGAAG GCCATGTAAT TCACCGCTGG 
GTGCCTTGCA GCAGAGACCC TTGTAATAGG TCCCACATTG ACAAAACAAT CCTGCTGATT 
AAGATTGAGG ACAAATATGT TGCTGTGATT GAAACTGGTG TGCTTGAACT CGGAGCTGAA 
GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004917308.1
CDS	272..7297
Translation	MGSQTLQILRQGVWASITGGWYYDPHQSTFVNTLHLYIWLFLLCFPFTLYMALPTSLVIVAVYCPVVAAVFIVLKLVNYRLHRALDEGEIVERSASELTERVAKAEHSDSSARREDSNGPGDPGGGIEMSEFVREATPPVGCSSRNSYSGLDPSNQVGVSRYGGASGSKGDTDAAKTSDDISLSLGQSYSLCKEASEDQDLTVDQKLFRLVSNDSFVSMQPSISSGGKDLTRDFSDKGSIPTHSHHHHVDLSLSNACDTELASLVPLHSHSYRKDHRPRGVPRTSSSAVAFPDTSLTDFSLYQPRRGLDPVSELEASKPHSGSKDSLVHNACASGIFQLDCLKSKGAQQLSRSGSVKHMKTDKSMDSLRSLSTRSSGSTESYCSGTDRDTNSTVSSYKSEQTSSTHVESILSEHEDVTAAKRVHCSDLEEEHSSATDKRTSSIRTALERNTAQEAKDANTSDDVQSPRGLSSSASEEANKNPHANELTVQTDWLPGKLSVNIDEPGEKPVILVDLRSLKETGIKQRDNDVRPKSSSLIHRTSSAHKSCRRRTGKKRASSFDASRHKEYVSFQGIADTKPHSTGFCHDEDSSDQSDLSRTSSVQSAHRFSSDSSSSTTSHSCQSPEARYSALRTKYRIKERGTDSHHTHLNCEGTSKKRSNRRTSSASGTKSRARVLSLDSGTVVSLSDANRLAAPENIKTLTTSKSDLEAKEGEVLDQMSLLGRASQLESVTRSRNSLPSQVTFQDVGERDASVGAHASEETVTFRRERSTFRRQAVRRRHNAGSNPTPPASLIGSPLSLQDGHQGQQSSTQVKVQSRPPSQAAVLSASASLLVRNGSVHLEASHDNASAVGSSSLHDELGMQALCNLGDMEVLMQHILGKFTPSLYETGGCDMSLVNFEPAARRTSNNLCDTDSHVSSSTSVRFYPHDLLSLPQIRLNRLLTIDTDLLEQQDIDLSPDLQDQMQPQEQAAQKVKHYYRFWILPKLWVGINFDRLTLLALFDRNREVLENVLSVILAILVAFLGSVLLIEGFFKDIWVFQFCLVIASCQYSLLKSVQPDSSSPRHGHNRIIAYSRPVYFCACCGLIWLLDYGSKNMSTTKYKLYGMAFTNPLLLVSARDLVIVFTLCFPVVFFIGLLPQVNTFVMYLCEQVDIHVFGGNATTSLLAAIYSFIRSIVAIALLYGLCYGALKDPWDAQHIPVLFSIFCGVLVAVSYHLSRQSSDPSVLISLVQSKIFPNSEDTNPEDPLSEVKDPLPEKLRSSVNERLQSDVVVCVVIAVLYFAIHVSTVFTALQPVLSYVLYALVGSVGLLTHYILPQVRKQLPWHCFSHPLLKTKEYYQFEVRNAAHLMWFEKAHIWLLFIEKNIIYPLIVLNELSIGAKDIVSPKKLDAEVGALLITIAGMKLLRSSFSSPTCQYATVIFTVLFFTFDYKEYTEKLLLDLFFMSILFSKLWELFFKLRFVYTYIAPWQITWGSAFHAFAQPFAVPHSAMLFVQAIVSALFSTPLNPFLGSAIFITSYVRPVKFWERDYNTKRVDHSNTRLASQLDRNPGSDDNNLNSIFYEHLTRSLQHSLCGDLLLGRWGNYNTGDCFILASDYLNALVHLIEIGNGLVTFQLRGLEFRGTYCQQREVEAITEGVEEDEGFCCCEPGHIPHMLSFNAAFGQRWLAWEVLVTKYVLEGYSITDNSAASMLQVFDLRRILTTYYVKGIIYYVTASAKLEEWLANETMQEGLRQCSDHCYVDVDPTFNPNIDEDYDLRLAGISRESFCASYLNWIKYCSSRREKPLDAEKDSPLVTLCYGLCILGRRALGTASHHMSSNLESFLYGLHALFKGDFRISSLRDEWIFADMELLRKVVVPGIRMSLKLHQDHFTSPDEYDDPAVLYEAITSHEQNLVIAHEGDPAWRSAVLSNSPSLLALRHVMDDGTNEYKIIMLNKRYLSFRVIKVNKECVRGLWAGQQQELVFLRNRNPERGSIQNAKQALRNMINSSCDQPIGYPIYVSPLTTSYSDSHDQLKQIFGGPISLANIRNFIVSTWHRLRKGCGAGCNSGGNIEDSDTGGGTSINSNNPPNANETQSSLSQVGVPGGTGHVPNARLQPPGMPHPSTLGTSHSSHSMQSSLVRHSPARGSVASQSSYCYNSRHSSLRTSTTGFVPCRRSSTSQISLRNLPSSIQSRLSMVNQTDPTGQSVLVGLPSSSSSSQSIPACKRHTLVGFLGTEGGNNLTETQSCGNSSPATHSRAKKEEVVYRVQIVDPSQVLEGVNISKRKELQWPDELVRLKAGKNSWKDWSPQEGMEGHVIHRWVPCSRDPCNRSHIDKTILLIKIEDKYVAVIETGVLELGAEV

Target ORF information:

RefSeq Version	XM_004917251.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pecanex homolog (pcnx), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004917251.3

ORF Insert Sequence:

ATGGGTTCCC AGACACTGCA AATCCTCCGG CAGGGAGTCT GGGCTTCCAT CACCGGAGGC 
TGGTACTACG ACCCCCACCA GAGTACCTTT GTCAATACCC TACACCTCTA CATCTGGCTC 
TTCCTCCTCT GCTTCCCCTT CACCCTCTAC ATGGCACTAC CCACATCTTT GGTGATTGTT 
GCTGTGTACT GCCCAGTCGT TGCAGCTGTT TTTATTGTTT TGAAGCTTGT TAACTACCGT 
CTGCACAGGG CATTGGATGA AGGAGAAATT GTGGAGAGGA GTGCTAGTGA GTTGACTGAG 
AGGGTGGCAA AGGCAGAACA CAGTGACAGC TCTGCAAGAA GAGAGGACAG CAATGGACCT 
GGTGATCCAG GTGGAGGGAT TGAAATGTCT GAATTTGTTC GAGAAGCAAC TCCTCCAGTT 
GGATGCAGTT CACGTAATTC CTACTCTGGG TTGGACCCAA GCAATCAGGT TGGTGTGTCT 
CGTTATGGAG GTGCATCTGG AAGCAAAGGG GATACAGATG CTGCAAAGAC ATCAGATGAT 
ATCAGTCTGA GTCTTGGCCA GAGTTACAGT TTGTGCAAAG AAGCAAGTGA AGACCAAGAT 
TTGACTGTGG ATCAGAAGCT TTTCCGTCTC GTCTCCAATG ACTCCTTTGT CTCCATGCAG 
CCATCTATAT CCTCGGGTGG AAAAGACTTA ACTAGAGACT TCAGTGACAA AGGATCAATC 
CCAACACACA GTCATCATCA CCATGTGGAT CTGTCTCTGT CTAATGCTTG TGACACAGAA 
TTGGCCTCAC TTGTTCCATT GCATTCACAT TCGTACAGGA AAGATCATCG ACCAAGAGGT 
GTCCCAAGGA CTTCAAGTTC TGCTGTTGCT TTCCCAGACA CTTCTCTCAC TGACTTTTCA 
CTCTATCAAC CAAGGCGCGG GCTGGATCCT GTTAGTGAAC TTGAAGCTTC CAAACCACAT 
TCTGGGTCCA AAGATTCACT AGTACATAAT GCTTGTGCTT CAGGGATTTT TCAATTAGAC 
TGTCTAAAAA GCAAAGGAGC CCAGCAGCTT TCAAGAAGTG GTAGTGTTAA ACACATGAAG 
ACTGACAAAA GCATGGATAG TTTAAGAAGC CTCAGCACGA GAAGCAGTGG ATCCACAGAA 
AGTTATTGTA GTGGAACAGA TCGGGACACA AATAGCACTG TTAGCAGCTA CAAAAGTGAA 
CAGACCAGTT CAACTCATGT TGAAAGTATT TTATCTGAGC ATGAGGATGT TACTGCTGCA 
AAGAGAGTCC ATTGTAGTGA CCTGGAGGAA GAACATAGCT CTGCTACTGA TAAAAGGACT 
AGTAGTATAA GAACTGCTTT AGAAAGAAAC ACTGCTCAGG AAGCCAAGGA TGCCAACACA 
TCTGATGATG TACAAAGTCC AAGGGGTCTT AGCTCATCAG CATCAGAGGA AGCTAATAAG 
AATCCACACG CAAATGAGTT AACTGTACAA ACAGACTGGC TTCCAGGTAA ACTGTCAGTG 
AACATTGATG AACCTGGGGA GAAACCAGTC ATTTTAGTTG ACTTGAGATC TTTGAAAGAA 
ACGGGTATTA AGCAGAGAGA CAATGACGTA CGACCCAAAT CATCTAGTTT AATACATCGA 
ACATCCTCTG CTCATAAATC ATGCAGGAGA CGGACAGGTA AAAAAAGAGC CAGCAGTTTT 
GATGCAAGTA GACACAAAGA ATATGTTTCC TTTCAAGGTA TAGCTGATAC CAAACCTCAC 
AGTACTGGCT TTTGTCATGA TGAAGATTCT AGCGACCAAA GCGACTTGAG CAGGACCTCA 
AGCGTTCAGT CGGCCCACCG TTTCAGCAGC GATAGCTCTT CCAGCACTAC TTCCCATTCA 
TGCCAGTCTC CTGAAGCAAG ATACAGTGCC CTAAGGACAA AATATCGAAT AAAAGAAAGA 
GGCACAGATT CACATCACAC ACACCTAAAT TGTGAAGGAA CTAGTAAAAA GCGGTCCAAC 
CGCAGGACTT CTAGTGCAAG TGGTACAAAA AGCCGAGCTA GAGTATTAAG TCTGGACAGT 
GGCACTGTAG TTAGTTTGAG TGATGCCAAC AGACTGGCCG CTCCTGAAAA TATAAAAACC 
TTAACCACTT CAAAATCAGA TCTAGAAGCC AAAGAAGGCG AAGTGCTAGA TCAGATGTCT 
CTGTTGGGGC GAGCTTCACA GTTAGAATCT GTAACACGGT CAAGAAATAG TTTGCCCAGC 
CAGGTTACTT TCCAAGATGT TGGAGAGCGA GACGCATCTG TTGGAGCACA CGCCAGTGAG 
GAGACAGTAA CATTTCGCCG CGAACGCAGC ACATTTAGGC GCCAAGCGGT TCGGCGCAGA 
CACAATGCAG GGAGTAATCC CACCCCTCCT GCGTCACTCA TCGGATCACC TCTGAGCCTT 
CAAGATGGTC ATCAAGGCCA GCAGTCATCA ACCCAGGTCA AAGTACAGTC CCGCCCCCCT 
TCCCAGGCTG CAGTGCTCAG CGCTAGCGCC TCCTTGCTGG TGAGAAATGG GAGTGTCCAC 
TTAGAAGCGT CTCATGACAA TGCATCTGCC GTAGGCAGTA GCAGTTTGCA CGATGAACTT 
GGTATGCAGG CCTTATGTAA CCTTGGTGAC ATGGAAGTTT TAATGCAGCA CATTTTGGGT 
AAATTTACCC CATCTCTATA TGAGACTGGA GGTTGTGACA TGTCACTTGT AAACTTTGAG 
CCAGCTGCAA GAAGGACGTC TAATAATCTT TGTGACACAG ATTCCCATGT ATCAAGTTCT 
ACCTCAGTTC GGTTCTACCC ACATGATCTA CTCTCTCTTC CCCAGATCCG ATTAAACAGG 
CTGCTAACCA TAGATACAGA TCTGTTAGAG CAGCAGGATA TTGATCTAAG CCCTGACCTG 
CAGGACCAGA TGCAACCACA GGAACAGGCT GCCCAAAAAG TAAAACACTA CTACCGGTTT 
TGGATTCTCC CAAAGCTGTG GGTCGGCATC AACTTTGACA GGCTTACACT CTTAGCCTTG 
TTCGATAGGA ACCGCGAGGT CCTAGAAAAT GTGTTGTCTG TCATTCTCGC CATTCTAGTT 
GCCTTTTTGG GCTCTGTTCT TCTCATAGAA GGCTTCTTTA AAGATATCTG GGTCTTCCAG 
TTTTGTCTTG TTATAGCCAG CTGTCAATAC TCTCTCCTTA AGAGTGTTCA ACCAGATTCT 
TCTTCCCCAA GACATGGCCA TAATCGTATC ATTGCCTATA GTAGGCCCGT TTACTTCTGT 
GCCTGTTGCG GCCTTATTTG GTTATTGGAT TATGGAAGCA AAAACATGTC TACCACCAAA 
TACAAACTAT ATGGAATGGC CTTCACCAAT CCTCTGCTTC TAGTGTCAGC CAGGGACTTA 
GTCATTGTCT TCACACTCTG TTTCCCAGTC GTGTTCTTTA TTGGTCTCCT GCCTCAGGTG 
AACACATTCG TCATGTACCT GTGTGAGCAA GTAGATATAC ATGTGTTTGG TGGAAATGCT 
ACCACCAGCT TGCTTGCAGC AATTTACAGC TTTATCCGTA GCATTGTGGC CATAGCTTTA 
CTCTACGGGC TGTGTTACGG TGCTCTTAAG GATCCATGGG ATGCACAACA CATTCCTGTA 
TTATTCTCTA TTTTCTGTGG TGTTCTTGTG GCAGTTTCAT ACCATCTCAG CCGACAGAGC 
AGCGATCCCT CTGTCCTCAT CTCACTGGTG CAATCAAAGA TTTTTCCAAA CAGCGAGGAT 
ACAAATCCTG AAGACCCACT ATCAGAAGTG AAAGACCCTC TTCCAGAAAA ACTGAGAAGC 
TCTGTTAATG AACGACTTCA GTCAGACGTA GTAGTCTGTG TCGTTATTGC AGTGTTGTAC 
TTTGCAATTC ATGTCAGCAC CGTATTTACT GCATTACAGC CGGTTCTGAG CTACGTACTC 
TATGCACTAG TTGGTTCTGT TGGACTCCTT ACTCACTACA TTCTACCACA AGTACGGAAA 
CAGCTTCCTT GGCACTGCTT CTCCCACCCG CTGCTGAAAA CTAAGGAATA TTACCAATTT 
GAAGTTCGAA ATGCTGCTCA TCTGATGTGG TTTGAGAAGG CTCACATCTG GCTGCTTTTT 
ATCGAGAAGA ACATTATTTA TCCTCTTATA GTTCTCAATG AGCTTAGCAT TGGTGCTAAG 
GATATCGTCA GCCCGAAAAA GCTGGATGCA GAAGTTGGTG CATTGTTGAT CACCATTGCC 
GGAATGAAGC TGCTGAGGTC GTCATTTAGC AGTCCCACCT GCCAGTATGC CACTGTCATT 
TTCACTGTCC TCTTCTTTAC TTTTGACTAC AAGGAGTATA CAGAAAAACT GCTGCTAGAT 
CTGTTCTTTA TGTCAATCCT CTTTAGCAAG CTCTGGGAAC TGTTTTTCAA ACTGCGCTTT 
GTCTACACCT ATATTGCCCC TTGGCAGATC ACGTGGGGAT CAGCATTCCA TGCTTTCGCA 
CAGCCATTTG CTGTACCTCA TTCTGCAATG CTGTTTGTAC AGGCAATTGT TTCTGCACTG 
TTCTCCACTC CCCTGAACCC TTTCTTAGGG AGTGCAATCT TTATTACATC ATATGTACGC 
CCTGTCAAGT TTTGGGAGAG AGACTACAAT ACAAAGAGAG TTGACCACTC AAACACAAGA 
TTAGCCTCTC AGCTTGACAG AAATCCAGGT TCAGATGACA ACAACTTGAA TTCCATATTT 
TATGAGCATC TCACACGCTC CCTTCAGCAT AGTCTGTGTG GTGATCTGTT GCTTGGCCGC 
TGGGGAAATT ACAATACTGG GGATTGCTTT ATTCTGGCCT CAGATTATCT AAACGCCTTG 
GTGCACCTTA TCGAAATAGG CAATGGTCTG GTCACCTTCC AGCTAAGAGG ACTAGAGTTC 
AGAGGTACCT ACTGCCAGCA GCGTGAAGTT GAAGCAATCA CTGAGGGTGT AGAGGAAGAT 
GAAGGCTTTT GCTGTTGCGA ACCTGGACAT ATTCCTCATA TGCTGTCTTT CAATGCTGCC 
TTTGGGCAGC GCTGGCTGGC ATGGGAAGTC TTGGTTACAA AGTATGTTCT GGAAGGTTAC 
AGCATCACAG ACAACAGTGC AGCCTCTATG CTTCAAGTGT TTGACCTTCG CAGGATCCTC 
ACTACCTATT ATGTTAAGGG CATTATATAC TATGTGACAG CATCTGCAAA ACTGGAGGAG 
TGGTTAGCAA ATGAGACAAT GCAGGAAGGT CTTCGCCAGT GTTCTGATCA TTGTTATGTT 
GATGTAGATC CAACTTTCAA CCCAAATATT GATGAAGACT ATGACCTTCG CTTAGCAGGC 
ATTTCACGGG AGAGCTTCTG TGCAAGCTAT CTTAACTGGA TTAAGTACTG TTCCTCTAGA 
AGGGAGAAGC CTCTTGATGC TGAAAAGGAT TCACCTTTGG TAACACTTTG TTATGGGCTC 
TGTATTTTGG GGAGAAGGGC ACTTGGTACA GCTTCTCATC ACATGTCCAG CAATTTGGAG 
TCCTTTCTAT ATGGTTTACA TGCCTTGTTT AAAGGAGATT TCCGCATATC ATCTCTCCGT 
GATGAGTGGA TTTTTGCTGA TATGGAGCTT TTAAGAAAAG TAGTAGTTCC TGGCATACGT 
ATGTCACTAA AACTTCATCA GGACCACTTT ACATCTCCAG ATGAGTATGA TGATCCTGCA 
GTTTTGTATG AAGCCATTAC ATCACATGAG CAGAACCTTG TTATTGCGCA TGAGGGAGAT 
CCAGCGTGGA GGAGTGCTGT TCTTTCTAAC TCACCTTCTC TTCTCGCGCT GCGTCATGTG 
ATGGATGATG GAACCAACGA ATACAAGATC ATAATGTTAA ATAAACGTTA TCTTAGTTTT 
AGAGTCATTA AGGTAAACAA GGAGTGTGTG CGGGGCTTAT GGGCTGGTCA GCAGCAAGAA 
TTGGTCTTTC TTCGTAATCG GAACCCTGAA AGAGGCAGCA TCCAAAATGC AAAGCAGGCT 
CTCAGGAATA TGATCAATTC ATCATGTGAC CAACCTATTG GATACCCAAT TTATGTTTCT 
CCACTGACAA CCTCTTATTC AGACAGCCAT GATCAGCTTA AACAGATATT TGGTGGGCCA 
ATTAGCTTGG CCAATATCCG GAACTTCATT GTATCTACAT GGCACAGATT AAGAAAAGGT 
TGCGGTGCAG GATGCAATAG TGGTGGAAAT ATTGAGGACT CTGACACTGG AGGCGGGACC 
TCCATTAATA GCAACAATCC ACCAAATGCA AATGAAACTC AGAGCAGTCT GTCACAAGTT 
GGTGTCCCAG GTGGAACAGG GCATGTGCCG AATGCACGGC TCCAGCCTCC GGGGATGCCT 
CATCCATCAA CACTAGGAAC CAGTCACAGT TCTCACTCCA TGCAGTCAAG CCTTGTCAGA 
CATTCCCCAG CTCGTGGATC AGTAGCAAGT CAATCATCTT ACTGCTACAA CAGCCGTCAC 
TCATCACTTA GAACATCCAC AACAGGATTT GTGCCTTGTA GGCGCTCCTC TACCAGCCAG 
ATATCTCTTC GCAATCTCCC CTCATCCATT CAGTCCCGTC TTTCCATGGT GAACCAAACA 
GACCCCACAG GACAGTCAGT TTTGGTTGGC CTTCCTTCCT CCAGCAGCTC AAGCCAGAGC 
ATCCCTGCAT GCAAAAGACA CACCTTAGTG GGCTTTTTAG GAACAGAAGG AGGCAATAAC 
CTCACTGAAA CGCAATCCTG TGGCAATTCT AGTCCAGCAA CCCATTCAAG AGCCAAAAAG 
GAAGAGGTTG TTTACAGAGT CCAGATTGTG GATCCAAGCC AAGTGCTTGA AGGCGTGAAT 
ATATCCAAAA GAAAGGAGCT GCAGTGGCCA GATGAGCTGG TGCGGCTAAA AGCCGGCAAG 
AACAGCTGGA AAGACTGGAG CCCTCAGGAA GGCATGGAAG GCCATGTAAT TCACCGCTGG 
GTGCCTTGCA GCAGAGACCC TTGTAATAGG TCCCACATTG ACAAAACAAT CCTGCTGATT 
AAGATTGAGG ACAAATATGT TGCTGTGATT GAAACTGGTG TGCTTGAACT CGGAGCTGAA 
GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pcnx1 ( XM_004917252.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004917309.1 Xenopus tropicalis pecanex homolog (pcnx), transcript variant X2, mRNA.

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CloneID	OXa15004
Clone ID Related Accession (Same CDS sequence)	XM_004917252.3
Accession Version	XM_004917252.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7011bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pecanex-like protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004917252.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCC AGACACTGCA AATCCTCCGG CAGGGAGTCT GGGCTTCCAT CACCGGAGGC 
TGGTACTACG ACCCCCACCA GAGTACCTTT GTCAATACCC TACACCTCTA CATCTGGCTC 
TTCCTCCTCT GCTTCCCCTT CACCCTCTAC ATGGCACTAC CCACATCTTT GGTGATTGTT 
GCTGTGTACT GCCCAGTCGT TGCAGCTGTT TTTATTGTTT TGAAGCTTGT TAACTACCGT 
CTGCACAGGG CATTGGATGA AGGAGAAATT GTGGAGAGGA GTGCTAGTGA GTTGACTGAG 
AGGGTGGCAA AGGCAGAACA CAGTGACAGC TCTGCAAGAA GAGAGGACAG CAATGGACCT 
GGTGATCCAG GTGGAGGGAT TGAAATGTCT GAATTTGTTC GAGAAGCAAC TCCTCCAGTT 
GGATGCAGTT CACGTAATTC CTACTCTGGG TTGGACCCAA GCAATCAGGT TGGTGTGTCT 
CGTTATGGAG GTGCATCTGG AAGCAAAGGG GATACAGATG CTGCAAAGAC ATCAGATGAT 
ATCAGTCTGA GTCTTGGCCA GAGTTACAGT TTGTGCAAAG AAGCAAGTGA AGACCAAGAT 
TTGACTGTGG ATCAGAAGCT TTTCCGTCTC GTCTCCAATG ACTCCTTTGT CTCCATGCAG 
CCATCTATAT CCTCGGGTGG AAAAGACTTA ACTAGAGACT TCAGTGACAA AGGATCAATC 
CCAACACACA GTCATCATCA CCATGTGGAT CTGTCTCTGT CTAATGCTTG TGACACAGAA 
TTGGCCTCAC TTGTTCCATT GCATTCACAT TCGTACAGGA AAGATCATCG ACCAAGAGGT 
GTCCCAAGGA CTTCAAGTTC TGCTGTTGCT TTCCCAGACA CTTCTCTCAC TGACTTTTCA 
CTCTATCAAC CAAGGCGCGG GCTGGATCCT GTTAGTGAAC TTGAAGCTTC CAAACCACAT 
TCTGGGTCCA AAGATTCACT AGTACATAAT GCTTGTGCTT CAGGGATTTT TCAATTAGAC 
TGTCTAAAAA GCAAAGGAGC CCAGCAGCTT TCAAGAAGTG GTAGTGTTAA ACACATGAAG 
ACTGACAAAA GCATGGATAG TTTAAGAAGC CTCAGCACGA GAAGCAGTGG ATCCACAGAA 
AGTTATTGTA GTGGAACAGA TCGGGACACA AATAGCACTG TTAGCAGCTA CAAAAGTGAA 
CAGACCAGTT CAACTCATGT TGAAAGTATT TTATCTGAGC ATGAGGATGT TACTGCTGCA 
AAGAGAGTCC ATTGTAGTGA CCTGGAGGAA GAACATAGCT CTGCTACTGA TAAAAGGACT 
AGTAGTATAA GAACTGCTTT AGAAAGAAAC ACTGCTCAGG AAGCCAAGGA TGCCAACACA 
TCTGATGATG TACAAAGTCC AAGGGGTCTT AGCTCATCAG CATCAGAGGA AGCTAATAAG 
AATCCACACG CAAATGAGTT AACTGTACAA ACAGACTGGC TTCCAGGTAA ACTGTCAGTG 
AACATTGATG AACCTGGGGA GAAACCAGTC ATTTTAGTTG ACTTGAGATC TTTGAAAGAA 
ACGGGTATTA AGCAGAGAGA CAATGACGTA CGACCCAAAT CATCTAGTTT AATACATCGA 
ACATCCTCTG CTCATAAATC ATGCAGGAGA CGGACAGGTA AAAAAAGAGC CAGCAGTTTT 
GATGCAAGTA GACACAAAGA ATATGTTTCC TTTCAAGGTA TAGCTGATAC CAAACCTCAC 
AGTACTGGCT TTTGTCATGA TGAAGATTCT AGCGACCAAA GCGACTTGAG CAGGACCTCA 
AGCGTTCAGT CGGCCCACCG TTTCAGCAGC GATAGCTCTT CCAGCACTAC TTCCCATTCA 
TGCCAGTCTC CTGAAGCAAG ATACAGTGCC CTAAGGACAA AATATCGAAT AAAAGAAAGA 
GGCACAGATT CACATCACAC ACACCTAAAT TGTGAAGGAA CTAGTAAAAA GCGGTCCAAC 
CGCAGGACTT CTAGTGCAAG TGGTACAAAA AGCCGAGCTA GAGTATTAAG TCTGGACAGT 
GGCACTGTAG TTAGTTTGAG TGATGCCAAC AGACTGGCCG CTCCTGAAAA TATAAAAACC 
TTAACCACTT CAAAATCAGA TCTAGAAGCC AAAGAAGGCG AAGTGCTAGA TCAGATGTCT 
CTGTTGGGGC GAGCTTCACA GTTAGAATCT GTAACACGGT CAAGAAATAG TTTGCCCAGC 
CAGGTTACTT TCCAAGATGT TGGAGAGCGA GACGCATCTG TTGGAGCACA CGCCAGTGAG 
GAGACAGTAA CATTTCGCCG CGAACGCAGC ACATTTAGGC GCCAAGCGGT TCGGCGCAGA 
CACAATGCAG GGAGTAATCC CACCCCTCCT GCGTCACTCA TCGGATCACC TCTGAGCCTT 
CAAGATGGTC ATCAAGGCCA GCAGTCATCA ACCCAGGTCA AAGTACAGTC CCGCCCCCCT 
TCCCAGGCTG CAGTGCTCAG CGCTAGCGCC TCCTTGCTGG TGAGAAATGG GAGTGTCCAC 
TTAGAAGCGT CTCATGACAA TGCATCTGCC GTAGGCAGTA GCAGTTTGCA CGATGAACTT 
GGTATGCAGG CCTTATGTAA CCTTGGTGAC ATGGAAGTTT TAATGCAGCA CATTTTGGGT 
AAATTTACCC CATCTCTATA TGAGACTGGA GGTTGTGACA TGTCACTTGT AAACTTTGAG 
CCAGCTGCAA GAAGGACGTC TAATAATCTT TGTGACACAG ATTCCCATGT ATCAAGTTCT 
ACCTCAGTTC GGTTCTACCC ACATGATCTA ATCCGATTAA ACAGGCTGCT AACCATAGAT 
ACAGATCTGT TAGAGCAGCA GGATATTGAT CTAAGCCCTG ACCTGCAGGA CCAGATGCAA 
CCACAGGAAC AGGCTGCCCA AAAAGTAAAA CACTACTACC GGTTTTGGAT TCTCCCAAAG 
CTGTGGGTCG GCATCAACTT TGACAGGCTT ACACTCTTAG CCTTGTTCGA TAGGAACCGC 
GAGGTCCTAG AAAATGTGTT GTCTGTCATT CTCGCCATTC TAGTTGCCTT TTTGGGCTCT 
GTTCTTCTCA TAGAAGGCTT CTTTAAAGAT ATCTGGGTCT TCCAGTTTTG TCTTGTTATA 
GCCAGCTGTC AATACTCTCT CCTTAAGAGT GTTCAACCAG ATTCTTCTTC CCCAAGACAT 
GGCCATAATC GTATCATTGC CTATAGTAGG CCCGTTTACT TCTGTGCCTG TTGCGGCCTT 
ATTTGGTTAT TGGATTATGG AAGCAAAAAC ATGTCTACCA CCAAATACAA ACTATATGGA 
ATGGCCTTCA CCAATCCTCT GCTTCTAGTG TCAGCCAGGG ACTTAGTCAT TGTCTTCACA 
CTCTGTTTCC CAGTCGTGTT CTTTATTGGT CTCCTGCCTC AGGTGAACAC ATTCGTCATG 
TACCTGTGTG AGCAAGTAGA TATACATGTG TTTGGTGGAA ATGCTACCAC CAGCTTGCTT 
GCAGCAATTT ACAGCTTTAT CCGTAGCATT GTGGCCATAG CTTTACTCTA CGGGCTGTGT 
TACGGTGCTC TTAAGGATCC ATGGGATGCA CAACACATTC CTGTATTATT CTCTATTTTC 
TGTGGTGTTC TTGTGGCAGT TTCATACCAT CTCAGCCGAC AGAGCAGCGA TCCCTCTGTC 
CTCATCTCAC TGGTGCAATC AAAGATTTTT CCAAACAGCG AGGATACAAA TCCTGAAGAC 
CCACTATCAG AAGTGAAAGA CCCTCTTCCA GAAAAACTGA GAAGCTCTGT TAATGAACGA 
CTTCAGTCAG ACGTAGTAGT CTGTGTCGTT ATTGCAGTGT TGTACTTTGC AATTCATGTC 
AGCACCGTAT TTACTGCATT ACAGCCGGTT CTGAGCTACG TACTCTATGC ACTAGTTGGT 
TCTGTTGGAC TCCTTACTCA CTACATTCTA CCACAAGTAC GGAAACAGCT TCCTTGGCAC 
TGCTTCTCCC ACCCGCTGCT GAAAACTAAG GAATATTACC AATTTGAAGT TCGAAATGCT 
GCTCATCTGA TGTGGTTTGA GAAGGCTCAC ATCTGGCTGC TTTTTATCGA GAAGAACATT 
ATTTATCCTC TTATAGTTCT CAATGAGCTT AGCATTGGTG CTAAGGATAT CGTCAGCCCG 
AAAAAGCTGG ATGCAGAAGT TGGTGCATTG TTGATCACCA TTGCCGGAAT GAAGCTGCTG 
AGGTCGTCAT TTAGCAGTCC CACCTGCCAG TATGCCACTG TCATTTTCAC TGTCCTCTTC 
TTTACTTTTG ACTACAAGGA GTATACAGAA AAACTGCTGC TAGATCTGTT CTTTATGTCA 
ATCCTCTTTA GCAAGCTCTG GGAACTGTTT TTCAAACTGC GCTTTGTCTA CACCTATATT 
GCCCCTTGGC AGATCACGTG GGGATCAGCA TTCCATGCTT TCGCACAGCC ATTTGCTGTA 
CCTCATTCTG CAATGCTGTT TGTACAGGCA ATTGTTTCTG CACTGTTCTC CACTCCCCTG 
AACCCTTTCT TAGGGAGTGC AATCTTTATT ACATCATATG TACGCCCTGT CAAGTTTTGG 
GAGAGAGACT ACAATACAAA GAGAGTTGAC CACTCAAACA CAAGATTAGC CTCTCAGCTT 
GACAGAAATC CAGGTTCAGA TGACAACAAC TTGAATTCCA TATTTTATGA GCATCTCACA 
CGCTCCCTTC AGCATAGTCT GTGTGGTGAT CTGTTGCTTG GCCGCTGGGG AAATTACAAT 
ACTGGGGATT GCTTTATTCT GGCCTCAGAT TATCTAAACG CCTTGGTGCA CCTTATCGAA 
ATAGGCAATG GTCTGGTCAC CTTCCAGCTA AGAGGACTAG AGTTCAGAGG TACCTACTGC 
CAGCAGCGTG AAGTTGAAGC AATCACTGAG GGTGTAGAGG AAGATGAAGG CTTTTGCTGT 
TGCGAACCTG GACATATTCC TCATATGCTG TCTTTCAATG CTGCCTTTGG GCAGCGCTGG 
CTGGCATGGG AAGTCTTGGT TACAAAGTAT GTTCTGGAAG GTTACAGCAT CACAGACAAC 
AGTGCAGCCT CTATGCTTCA AGTGTTTGAC CTTCGCAGGA TCCTCACTAC CTATTATGTT 
AAGGGCATTA TATACTATGT GACAGCATCT GCAAAACTGG AGGAGTGGTT AGCAAATGAG 
ACAATGCAGG AAGGTCTTCG CCAGTGTTCT GATCATTGTT ATGTTGATGT AGATCCAACT 
TTCAACCCAA ATATTGATGA AGACTATGAC CTTCGCTTAG CAGGCATTTC ACGGGAGAGC 
TTCTGTGCAA GCTATCTTAA CTGGATTAAG TACTGTTCCT CTAGAAGGGA GAAGCCTCTT 
GATGCTGAAA AGGATTCACC TTTGGTAACA CTTTGTTATG GGCTCTGTAT TTTGGGGAGA 
AGGGCACTTG GTACAGCTTC TCATCACATG TCCAGCAATT TGGAGTCCTT TCTATATGGT 
TTACATGCCT TGTTTAAAGG AGATTTCCGC ATATCATCTC TCCGTGATGA GTGGATTTTT 
GCTGATATGG AGCTTTTAAG AAAAGTAGTA GTTCCTGGCA TACGTATGTC ACTAAAACTT 
CATCAGGACC ACTTTACATC TCCAGATGAG TATGATGATC CTGCAGTTTT GTATGAAGCC 
ATTACATCAC ATGAGCAGAA CCTTGTTATT GCGCATGAGG GAGATCCAGC GTGGAGGAGT 
GCTGTTCTTT CTAACTCACC TTCTCTTCTC GCGCTGCGTC ATGTGATGGA TGATGGAACC 
AACGAATACA AGATCATAAT GTTAAATAAA CGTTATCTTA GTTTTAGAGT CATTAAGGTA 
AACAAGGAGT GTGTGCGGGG CTTATGGGCT GGTCAGCAGC AAGAATTGGT CTTTCTTCGT 
AATCGGAACC CTGAAAGAGG CAGCATCCAA AATGCAAAGC AGGCTCTCAG GAATATGATC 
AATTCATCAT GTGACCAACC TATTGGATAC CCAATTTATG TTTCTCCACT GACAACCTCT 
TATTCAGACA GCCATGATCA GCTTAAACAG ATATTTGGTG GGCCAATTAG CTTGGCCAAT 
ATCCGGAACT TCATTGTATC TACATGGCAC AGATTAAGAA AAGGTTGCGG TGCAGGATGC 
AATAGTGGTG GAAATATTGA GGACTCTGAC ACTGGAGGCG GGACCTCCAT TAATAGCAAC 
AATCCACCAA ATGCAAATGA AACTCAGAGC AGTCTGTCAC AAGTTGGTGT CCCAGGTGGA 
ACAGGGCATG TGCCGAATGC ACGGCTCCAG CCTCCGGGGA TGCCTCATCC ATCAACACTA 
GGAACCAGTC ACAGTTCTCA CTCCATGCAG TCAAGCCTTG TCAGACATTC CCCAGCTCGT 
GGATCAGTAG CAAGTCAATC ATCTTACTGC TACAACAGCC GTCACTCATC ACTTAGAACA 
TCCACAACAG GATTTGTGCC TTGTAGGCGC TCCTCTACCA GCCAGATATC TCTTCGCAAT 
CTCCCCTCAT CCATTCAGTC CCGTCTTTCC ATGGTGAACC AAACAGACCC CACAGGACAG 
TCAGTTTTGG TTGGCCTTCC TTCCTCCAGC AGCTCAAGCC AGAGCATCCC TGCATGCAAA 
AGACACACCT TAGTGGGCTT TTTAGGAACA GAAGGAGGCA ATAACCTCAC TGAAACGCAA 
TCCTGTGGCA ATTCTAGTCC AGCAACCCAT TCAAGAGCCA AAAAGGAAGA GGTTGTTTAC 
AGAGTCCAGA TTGTGGATCC AAGCCAAGTG CTTGAAGGCG TGAATATATC CAAAAGAAAG 
GAGCTGCAGT GGCCAGATGA GCTGGTGCGG CTAAAAGCCG GCAAGAACAG CTGGAAAGAC 
TGGAGCCCTC AGGAAGGCAT GGAAGGCCAT GTAATTCACC GCTGGGTGCC TTGCAGCAGA 
GACCCTTGTA ATAGGTCCCA CATTGACAAA ACAATCCTGC TGATTAAGAT TGAGGACAAA 
TATGTTGCTG TGATTGAAAC TGGTGTGCTT GAACTCGGAG CTGAAGTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004917309.1
CDS	272..7282
Translation	MGSQTLQILRQGVWASITGGWYYDPHQSTFVNTLHLYIWLFLLCFPFTLYMALPTSLVIVAVYCPVVAAVFIVLKLVNYRLHRALDEGEIVERSASELTERVAKAEHSDSSARREDSNGPGDPGGGIEMSEFVREATPPVGCSSRNSYSGLDPSNQVGVSRYGGASGSKGDTDAAKTSDDISLSLGQSYSLCKEASEDQDLTVDQKLFRLVSNDSFVSMQPSISSGGKDLTRDFSDKGSIPTHSHHHHVDLSLSNACDTELASLVPLHSHSYRKDHRPRGVPRTSSSAVAFPDTSLTDFSLYQPRRGLDPVSELEASKPHSGSKDSLVHNACASGIFQLDCLKSKGAQQLSRSGSVKHMKTDKSMDSLRSLSTRSSGSTESYCSGTDRDTNSTVSSYKSEQTSSTHVESILSEHEDVTAAKRVHCSDLEEEHSSATDKRTSSIRTALERNTAQEAKDANTSDDVQSPRGLSSSASEEANKNPHANELTVQTDWLPGKLSVNIDEPGEKPVILVDLRSLKETGIKQRDNDVRPKSSSLIHRTSSAHKSCRRRTGKKRASSFDASRHKEYVSFQGIADTKPHSTGFCHDEDSSDQSDLSRTSSVQSAHRFSSDSSSSTTSHSCQSPEARYSALRTKYRIKERGTDSHHTHLNCEGTSKKRSNRRTSSASGTKSRARVLSLDSGTVVSLSDANRLAAPENIKTLTTSKSDLEAKEGEVLDQMSLLGRASQLESVTRSRNSLPSQVTFQDVGERDASVGAHASEETVTFRRERSTFRRQAVRRRHNAGSNPTPPASLIGSPLSLQDGHQGQQSSTQVKVQSRPPSQAAVLSASASLLVRNGSVHLEASHDNASAVGSSSLHDELGMQALCNLGDMEVLMQHILGKFTPSLYETGGCDMSLVNFEPAARRTSNNLCDTDSHVSSSTSVRFYPHDLIRLNRLLTIDTDLLEQQDIDLSPDLQDQMQPQEQAAQKVKHYYRFWILPKLWVGINFDRLTLLALFDRNREVLENVLSVILAILVAFLGSVLLIEGFFKDIWVFQFCLVIASCQYSLLKSVQPDSSSPRHGHNRIIAYSRPVYFCACCGLIWLLDYGSKNMSTTKYKLYGMAFTNPLLLVSARDLVIVFTLCFPVVFFIGLLPQVNTFVMYLCEQVDIHVFGGNATTSLLAAIYSFIRSIVAIALLYGLCYGALKDPWDAQHIPVLFSIFCGVLVAVSYHLSRQSSDPSVLISLVQSKIFPNSEDTNPEDPLSEVKDPLPEKLRSSVNERLQSDVVVCVVIAVLYFAIHVSTVFTALQPVLSYVLYALVGSVGLLTHYILPQVRKQLPWHCFSHPLLKTKEYYQFEVRNAAHLMWFEKAHIWLLFIEKNIIYPLIVLNELSIGAKDIVSPKKLDAEVGALLITIAGMKLLRSSFSSPTCQYATVIFTVLFFTFDYKEYTEKLLLDLFFMSILFSKLWELFFKLRFVYTYIAPWQITWGSAFHAFAQPFAVPHSAMLFVQAIVSALFSTPLNPFLGSAIFITSYVRPVKFWERDYNTKRVDHSNTRLASQLDRNPGSDDNNLNSIFYEHLTRSLQHSLCGDLLLGRWGNYNTGDCFILASDYLNALVHLIEIGNGLVTFQLRGLEFRGTYCQQREVEAITEGVEEDEGFCCCEPGHIPHMLSFNAAFGQRWLAWEVLVTKYVLEGYSITDNSAASMLQVFDLRRILTTYYVKGIIYYVTASAKLEEWLANETMQEGLRQCSDHCYVDVDPTFNPNIDEDYDLRLAGISRESFCASYLNWIKYCSSRREKPLDAEKDSPLVTLCYGLCILGRRALGTASHHMSSNLESFLYGLHALFKGDFRISSLRDEWIFADMELLRKVVVPGIRMSLKLHQDHFTSPDEYDDPAVLYEAITSHEQNLVIAHEGDPAWRSAVLSNSPSLLALRHVMDDGTNEYKIIMLNKRYLSFRVIKVNKECVRGLWAGQQQELVFLRNRNPERGSIQNAKQALRNMINSSCDQPIGYPIYVSPLTTSYSDSHDQLKQIFGGPISLANIRNFIVSTWHRLRKGCGAGCNSGGNIEDSDTGGGTSINSNNPPNANETQSSLSQVGVPGGTGHVPNARLQPPGMPHPSTLGTSHSSHSMQSSLVRHSPARGSVASQSSYCYNSRHSSLRTSTTGFVPCRRSSTSQISLRNLPSSIQSRLSMVNQTDPTGQSVLVGLPSSSSSSQSIPACKRHTLVGFLGTEGGNNLTETQSCGNSSPATHSRAKKEEVVYRVQIVDPSQVLEGVNISKRKELQWPDELVRLKAGKNSWKDWSPQEGMEGHVIHRWVPCSRDPCNRSHIDKTILLIKIEDKYVAVIETGVLELGAEV

Target ORF information:

RefSeq Version	XM_004917252.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pecanex homolog (pcnx), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004917252.3

ORF Insert Sequence:

ATGGGTTCCC AGACACTGCA AATCCTCCGG CAGGGAGTCT GGGCTTCCAT CACCGGAGGC 
TGGTACTACG ACCCCCACCA GAGTACCTTT GTCAATACCC TACACCTCTA CATCTGGCTC 
TTCCTCCTCT GCTTCCCCTT CACCCTCTAC ATGGCACTAC CCACATCTTT GGTGATTGTT 
GCTGTGTACT GCCCAGTCGT TGCAGCTGTT TTTATTGTTT TGAAGCTTGT TAACTACCGT 
CTGCACAGGG CATTGGATGA AGGAGAAATT GTGGAGAGGA GTGCTAGTGA GTTGACTGAG 
AGGGTGGCAA AGGCAGAACA CAGTGACAGC TCTGCAAGAA GAGAGGACAG CAATGGACCT 
GGTGATCCAG GTGGAGGGAT TGAAATGTCT GAATTTGTTC GAGAAGCAAC TCCTCCAGTT 
GGATGCAGTT CACGTAATTC CTACTCTGGG TTGGACCCAA GCAATCAGGT TGGTGTGTCT 
CGTTATGGAG GTGCATCTGG AAGCAAAGGG GATACAGATG CTGCAAAGAC ATCAGATGAT 
ATCAGTCTGA GTCTTGGCCA GAGTTACAGT TTGTGCAAAG AAGCAAGTGA AGACCAAGAT 
TTGACTGTGG ATCAGAAGCT TTTCCGTCTC GTCTCCAATG ACTCCTTTGT CTCCATGCAG 
CCATCTATAT CCTCGGGTGG AAAAGACTTA ACTAGAGACT TCAGTGACAA AGGATCAATC 
CCAACACACA GTCATCATCA CCATGTGGAT CTGTCTCTGT CTAATGCTTG TGACACAGAA 
TTGGCCTCAC TTGTTCCATT GCATTCACAT TCGTACAGGA AAGATCATCG ACCAAGAGGT 
GTCCCAAGGA CTTCAAGTTC TGCTGTTGCT TTCCCAGACA CTTCTCTCAC TGACTTTTCA 
CTCTATCAAC CAAGGCGCGG GCTGGATCCT GTTAGTGAAC TTGAAGCTTC CAAACCACAT 
TCTGGGTCCA AAGATTCACT AGTACATAAT GCTTGTGCTT CAGGGATTTT TCAATTAGAC 
TGTCTAAAAA GCAAAGGAGC CCAGCAGCTT TCAAGAAGTG GTAGTGTTAA ACACATGAAG 
ACTGACAAAA GCATGGATAG TTTAAGAAGC CTCAGCACGA GAAGCAGTGG ATCCACAGAA 
AGTTATTGTA GTGGAACAGA TCGGGACACA AATAGCACTG TTAGCAGCTA CAAAAGTGAA 
CAGACCAGTT CAACTCATGT TGAAAGTATT TTATCTGAGC ATGAGGATGT TACTGCTGCA 
AAGAGAGTCC ATTGTAGTGA CCTGGAGGAA GAACATAGCT CTGCTACTGA TAAAAGGACT 
AGTAGTATAA GAACTGCTTT AGAAAGAAAC ACTGCTCAGG AAGCCAAGGA TGCCAACACA 
TCTGATGATG TACAAAGTCC AAGGGGTCTT AGCTCATCAG CATCAGAGGA AGCTAATAAG 
AATCCACACG CAAATGAGTT AACTGTACAA ACAGACTGGC TTCCAGGTAA ACTGTCAGTG 
AACATTGATG AACCTGGGGA GAAACCAGTC ATTTTAGTTG ACTTGAGATC TTTGAAAGAA 
ACGGGTATTA AGCAGAGAGA CAATGACGTA CGACCCAAAT CATCTAGTTT AATACATCGA 
ACATCCTCTG CTCATAAATC ATGCAGGAGA CGGACAGGTA AAAAAAGAGC CAGCAGTTTT 
GATGCAAGTA GACACAAAGA ATATGTTTCC TTTCAAGGTA TAGCTGATAC CAAACCTCAC 
AGTACTGGCT TTTGTCATGA TGAAGATTCT AGCGACCAAA GCGACTTGAG CAGGACCTCA 
AGCGTTCAGT CGGCCCACCG TTTCAGCAGC GATAGCTCTT CCAGCACTAC TTCCCATTCA 
TGCCAGTCTC CTGAAGCAAG ATACAGTGCC CTAAGGACAA AATATCGAAT AAAAGAAAGA 
GGCACAGATT CACATCACAC ACACCTAAAT TGTGAAGGAA CTAGTAAAAA GCGGTCCAAC 
CGCAGGACTT CTAGTGCAAG TGGTACAAAA AGCCGAGCTA GAGTATTAAG TCTGGACAGT 
GGCACTGTAG TTAGTTTGAG TGATGCCAAC AGACTGGCCG CTCCTGAAAA TATAAAAACC 
TTAACCACTT CAAAATCAGA TCTAGAAGCC AAAGAAGGCG AAGTGCTAGA TCAGATGTCT 
CTGTTGGGGC GAGCTTCACA GTTAGAATCT GTAACACGGT CAAGAAATAG TTTGCCCAGC 
CAGGTTACTT TCCAAGATGT TGGAGAGCGA GACGCATCTG TTGGAGCACA CGCCAGTGAG 
GAGACAGTAA CATTTCGCCG CGAACGCAGC ACATTTAGGC GCCAAGCGGT TCGGCGCAGA 
CACAATGCAG GGAGTAATCC CACCCCTCCT GCGTCACTCA TCGGATCACC TCTGAGCCTT 
CAAGATGGTC ATCAAGGCCA GCAGTCATCA ACCCAGGTCA AAGTACAGTC CCGCCCCCCT 
TCCCAGGCTG CAGTGCTCAG CGCTAGCGCC TCCTTGCTGG TGAGAAATGG GAGTGTCCAC 
TTAGAAGCGT CTCATGACAA TGCATCTGCC GTAGGCAGTA GCAGTTTGCA CGATGAACTT 
GGTATGCAGG CCTTATGTAA CCTTGGTGAC ATGGAAGTTT TAATGCAGCA CATTTTGGGT 
AAATTTACCC CATCTCTATA TGAGACTGGA GGTTGTGACA TGTCACTTGT AAACTTTGAG 
CCAGCTGCAA GAAGGACGTC TAATAATCTT TGTGACACAG ATTCCCATGT ATCAAGTTCT 
ACCTCAGTTC GGTTCTACCC ACATGATCTA ATCCGATTAA ACAGGCTGCT AACCATAGAT 
ACAGATCTGT TAGAGCAGCA GGATATTGAT CTAAGCCCTG ACCTGCAGGA CCAGATGCAA 
CCACAGGAAC AGGCTGCCCA AAAAGTAAAA CACTACTACC GGTTTTGGAT TCTCCCAAAG 
CTGTGGGTCG GCATCAACTT TGACAGGCTT ACACTCTTAG CCTTGTTCGA TAGGAACCGC 
GAGGTCCTAG AAAATGTGTT GTCTGTCATT CTCGCCATTC TAGTTGCCTT TTTGGGCTCT 
GTTCTTCTCA TAGAAGGCTT CTTTAAAGAT ATCTGGGTCT TCCAGTTTTG TCTTGTTATA 
GCCAGCTGTC AATACTCTCT CCTTAAGAGT GTTCAACCAG ATTCTTCTTC CCCAAGACAT 
GGCCATAATC GTATCATTGC CTATAGTAGG CCCGTTTACT TCTGTGCCTG TTGCGGCCTT 
ATTTGGTTAT TGGATTATGG AAGCAAAAAC ATGTCTACCA CCAAATACAA ACTATATGGA 
ATGGCCTTCA CCAATCCTCT GCTTCTAGTG TCAGCCAGGG ACTTAGTCAT TGTCTTCACA 
CTCTGTTTCC CAGTCGTGTT CTTTATTGGT CTCCTGCCTC AGGTGAACAC ATTCGTCATG 
TACCTGTGTG AGCAAGTAGA TATACATGTG TTTGGTGGAA ATGCTACCAC CAGCTTGCTT 
GCAGCAATTT ACAGCTTTAT CCGTAGCATT GTGGCCATAG CTTTACTCTA CGGGCTGTGT 
TACGGTGCTC TTAAGGATCC ATGGGATGCA CAACACATTC CTGTATTATT CTCTATTTTC 
TGTGGTGTTC TTGTGGCAGT TTCATACCAT CTCAGCCGAC AGAGCAGCGA TCCCTCTGTC 
CTCATCTCAC TGGTGCAATC AAAGATTTTT CCAAACAGCG AGGATACAAA TCCTGAAGAC 
CCACTATCAG AAGTGAAAGA CCCTCTTCCA GAAAAACTGA GAAGCTCTGT TAATGAACGA 
CTTCAGTCAG ACGTAGTAGT CTGTGTCGTT ATTGCAGTGT TGTACTTTGC AATTCATGTC 
AGCACCGTAT TTACTGCATT ACAGCCGGTT CTGAGCTACG TACTCTATGC ACTAGTTGGT 
TCTGTTGGAC TCCTTACTCA CTACATTCTA CCACAAGTAC GGAAACAGCT TCCTTGGCAC 
TGCTTCTCCC ACCCGCTGCT GAAAACTAAG GAATATTACC AATTTGAAGT TCGAAATGCT 
GCTCATCTGA TGTGGTTTGA GAAGGCTCAC ATCTGGCTGC TTTTTATCGA GAAGAACATT 
ATTTATCCTC TTATAGTTCT CAATGAGCTT AGCATTGGTG CTAAGGATAT CGTCAGCCCG 
AAAAAGCTGG ATGCAGAAGT TGGTGCATTG TTGATCACCA TTGCCGGAAT GAAGCTGCTG 
AGGTCGTCAT TTAGCAGTCC CACCTGCCAG TATGCCACTG TCATTTTCAC TGTCCTCTTC 
TTTACTTTTG ACTACAAGGA GTATACAGAA AAACTGCTGC TAGATCTGTT CTTTATGTCA 
ATCCTCTTTA GCAAGCTCTG GGAACTGTTT TTCAAACTGC GCTTTGTCTA CACCTATATT 
GCCCCTTGGC AGATCACGTG GGGATCAGCA TTCCATGCTT TCGCACAGCC ATTTGCTGTA 
CCTCATTCTG CAATGCTGTT TGTACAGGCA ATTGTTTCTG CACTGTTCTC CACTCCCCTG 
AACCCTTTCT TAGGGAGTGC AATCTTTATT ACATCATATG TACGCCCTGT CAAGTTTTGG 
GAGAGAGACT ACAATACAAA GAGAGTTGAC CACTCAAACA CAAGATTAGC CTCTCAGCTT 
GACAGAAATC CAGGTTCAGA TGACAACAAC TTGAATTCCA TATTTTATGA GCATCTCACA 
CGCTCCCTTC AGCATAGTCT GTGTGGTGAT CTGTTGCTTG GCCGCTGGGG AAATTACAAT 
ACTGGGGATT GCTTTATTCT GGCCTCAGAT TATCTAAACG CCTTGGTGCA CCTTATCGAA 
ATAGGCAATG GTCTGGTCAC CTTCCAGCTA AGAGGACTAG AGTTCAGAGG TACCTACTGC 
CAGCAGCGTG AAGTTGAAGC AATCACTGAG GGTGTAGAGG AAGATGAAGG CTTTTGCTGT 
TGCGAACCTG GACATATTCC TCATATGCTG TCTTTCAATG CTGCCTTTGG GCAGCGCTGG 
CTGGCATGGG AAGTCTTGGT TACAAAGTAT GTTCTGGAAG GTTACAGCAT CACAGACAAC 
AGTGCAGCCT CTATGCTTCA AGTGTTTGAC CTTCGCAGGA TCCTCACTAC CTATTATGTT 
AAGGGCATTA TATACTATGT GACAGCATCT GCAAAACTGG AGGAGTGGTT AGCAAATGAG 
ACAATGCAGG AAGGTCTTCG CCAGTGTTCT GATCATTGTT ATGTTGATGT AGATCCAACT 
TTCAACCCAA ATATTGATGA AGACTATGAC CTTCGCTTAG CAGGCATTTC ACGGGAGAGC 
TTCTGTGCAA GCTATCTTAA CTGGATTAAG TACTGTTCCT CTAGAAGGGA GAAGCCTCTT 
GATGCTGAAA AGGATTCACC TTTGGTAACA CTTTGTTATG GGCTCTGTAT TTTGGGGAGA 
AGGGCACTTG GTACAGCTTC TCATCACATG TCCAGCAATT TGGAGTCCTT TCTATATGGT 
TTACATGCCT TGTTTAAAGG AGATTTCCGC ATATCATCTC TCCGTGATGA GTGGATTTTT 
GCTGATATGG AGCTTTTAAG AAAAGTAGTA GTTCCTGGCA TACGTATGTC ACTAAAACTT 
CATCAGGACC ACTTTACATC TCCAGATGAG TATGATGATC CTGCAGTTTT GTATGAAGCC 
ATTACATCAC ATGAGCAGAA CCTTGTTATT GCGCATGAGG GAGATCCAGC GTGGAGGAGT 
GCTGTTCTTT CTAACTCACC TTCTCTTCTC GCGCTGCGTC ATGTGATGGA TGATGGAACC 
AACGAATACA AGATCATAAT GTTAAATAAA CGTTATCTTA GTTTTAGAGT CATTAAGGTA 
AACAAGGAGT GTGTGCGGGG CTTATGGGCT GGTCAGCAGC AAGAATTGGT CTTTCTTCGT 
AATCGGAACC CTGAAAGAGG CAGCATCCAA AATGCAAAGC AGGCTCTCAG GAATATGATC 
AATTCATCAT GTGACCAACC TATTGGATAC CCAATTTATG TTTCTCCACT GACAACCTCT 
TATTCAGACA GCCATGATCA GCTTAAACAG ATATTTGGTG GGCCAATTAG CTTGGCCAAT 
ATCCGGAACT TCATTGTATC TACATGGCAC AGATTAAGAA AAGGTTGCGG TGCAGGATGC 
AATAGTGGTG GAAATATTGA GGACTCTGAC ACTGGAGGCG GGACCTCCAT TAATAGCAAC 
AATCCACCAA ATGCAAATGA AACTCAGAGC AGTCTGTCAC AAGTTGGTGT CCCAGGTGGA 
ACAGGGCATG TGCCGAATGC ACGGCTCCAG CCTCCGGGGA TGCCTCATCC ATCAACACTA 
GGAACCAGTC ACAGTTCTCA CTCCATGCAG TCAAGCCTTG TCAGACATTC CCCAGCTCGT 
GGATCAGTAG CAAGTCAATC ATCTTACTGC TACAACAGCC GTCACTCATC ACTTAGAACA 
TCCACAACAG GATTTGTGCC TTGTAGGCGC TCCTCTACCA GCCAGATATC TCTTCGCAAT 
CTCCCCTCAT CCATTCAGTC CCGTCTTTCC ATGGTGAACC AAACAGACCC CACAGGACAG 
TCAGTTTTGG TTGGCCTTCC TTCCTCCAGC AGCTCAAGCC AGAGCATCCC TGCATGCAAA 
AGACACACCT TAGTGGGCTT TTTAGGAACA GAAGGAGGCA ATAACCTCAC TGAAACGCAA 
TCCTGTGGCA ATTCTAGTCC AGCAACCCAT TCAAGAGCCA AAAAGGAAGA GGTTGTTTAC 
AGAGTCCAGA TTGTGGATCC AAGCCAAGTG CTTGAAGGCG TGAATATATC CAAAAGAAAG 
GAGCTGCAGT GGCCAGATGA GCTGGTGCGG CTAAAAGCCG GCAAGAACAG CTGGAAAGAC 
TGGAGCCCTC AGGAAGGCAT GGAAGGCCAT GTAATTCACC GCTGGGTGCC TTGCAGCAGA 
GACCCTTGTA ATAGGTCCCA CATTGACAAA ACAATCCTGC TGATTAAGAT TGAGGACAAA 
TATGTTGCTG TGATTGAAAC TGGTGTGCTT GAACTCGGAG CTGAAGTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pcnx1 ( XM_004917253.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004917310.1 Xenopus tropicalis pecanex homolog (pcnx), transcript variant X3, mRNA.

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CloneID	OXa15005
Clone ID Related Accession (Same CDS sequence)	XM_004917253.3
Accession Version	XM_004917253.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6969bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pecanex-like protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004917253.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCC AGACACTGCA AATCCTCCGG CAGGGAGTCT GGGCTTCCAT CACCGGAGGC 
TGGTACTACG ACCCCCACCA GAGTACCTTT GTCAATACCC TACACCTCTA CATCTGGCTC 
TTCCTCCTCT GCTTCCCCTT CACCCTCTAC ATGGCACTAC CCACATCTTT GGTGATTGTT 
GCTGTGTACT GCCCAGTCGT TGCAGCTGTT TTTATTGTTT TGAAGCTTGT TAACTACCGT 
CTGCACAGGG CATTGGATGA AGGAGAAATT GTGGAGAGGA GTGCTAGTGA GTTGACTGAG 
AGGGTGGCAA AGGCAGAACA CAGTGACAGC TCTGCAAGAA GAGAGGACAG CAATGGACCT 
GGTGATCCAG GTGGAGGGAT TGAAATGTCT GAATTTGTTC GAGAAGCAAC TCCTCCAGTT 
GGATGCAGTT CACGTAATTC CTACTCTGGG TTGGACCCAA GCAATCAGGT TGGTGTGTCT 
CGTTATGGAG GTGCATCTGG AAGCAAAGGG GATACAGATG CTGCAAAGAC ATCAGATGAT 
ATCAGTCTGA GTCTTGGCCA GAGTTACAGT TTGTGCAAAG AAGCAAGTGA AGACCAAGAT 
TTGACTGTGG ATCAGAAGCT TTTCCGTCTC GTCTCCAATG ACTCCTTTGT CTCCATGCAG 
CCATCTATAT CCTCGGGTGG AAAAGACTTA ACTAGAGACT TCAGTGACAA AGGATCAATC 
CCAACACACA GTCATCATCA CCATGTGGAT CTGTCTCTGT CTAATGCTTG TGACACAGAA 
TTGGCCTCAC TTGTTCCATT GCATTCACAT TCGTACAGGA AAGATCATCG ACCAAGAGGT 
GTCCCAAGGA CTTCAAGTTC TGCTGTTGCT TTCCCAGACA CTTCTCTCAC TGACTTTTCA 
CTCTATCAAC CAAGGCGCGG GCTGGATCCT GTTAGTGAAC TTGAAGCTTC CAAACCACAT 
TCTGGGTCCA AAGATTCACT AGTACATAAT GCTTGTGCTT CAGGGATTTT TCAATTAGAC 
TGTCTAAAAA GCAAAGGAGC CCAGCAGCTT TCAAGAAGTG GTAGTGTTAA ACACATGAAG 
ACTGACAAAA GCATGGATAG TTTAAGAAGC CTCAGCACGA GAAGCAGTGG ATCCACAGAA 
AGTTATTGTA GTGGAACAGA TCGGGACACA AATAGCACTG TTAGCAGCTA CAAAAGTGAA 
CAGACCAGTT CAACTCATGT TGAAAGTATT TTATCTGAGC ATGAGGATGT TACTGCTGCA 
AAGAGAGTCC ATTGTAGTGA CCTGGAGGAA GAACATAGCT CTGCTACTGA TAAAAGGACT 
AGTAGTATAA GAACTGCTTT AGAAAGAAAC ACTGCTCAGG AAGCCAAGGA TGCCAACACA 
TCTGATGATG TACAAAGTCC AAGGGGTCTT AGCTCATCAG CATCAGAGGA AGCTAATAAG 
AATCCACACG CAAATGAGTT AACTGTACAA ACAGACTGGC TTCCAGGTAA ACTGTCAGTG 
AACATTGATG AACCTGGGGA GAAACCAGTC ATTTTAGTTG ACTTGAGATC TTTGAAAGAA 
ACGGGTATTA AGCAGAGAGA CAATGACGTA CGACCCAAAT CATCTAGTTT AATACATCGA 
ACATCCTCTG CTCATAAATC ATGCAGGAGA CGGACAGGTA AAAAAAGAGC CAGCAGTTTT 
GATGCAAGTA GACACAAAGA ATATGTTTCC TTTCAAGGTA TAGCTGATAC CAAACCTCAC 
AGTACTGGCT TTTGTCATGA TGAAGATTCT AGCGACCAAA GCGACTTGAG CAGGACCTCA 
AGCGTTCAGT CGGCCCACCG TTTCAGCAGC GATAGCTCTT CCAGCACTAC TTCCCATTCA 
TGCCAGTCTC CTGAAGCAAG ATACAGTGCC CTAAGGACAA AATATCGAAT AAAAGAAAGA 
GGCACAGATT CACATCACAC ACACCTAAAT TGTGAAGGAA CTAGTAAAAA GCGGTCCAAC 
CGCAGGACTT CTAGTGCAAG TGGTACAAAA AGCCGAGCTA GAGTATTAAG TCTGGACAGT 
GGCACTGTAG TTAGTTTGAG TGATGCCAAC AGACTGGCCG CTCCTGAAAA TATAAAAACC 
TTAACCACTT CAAAATCAGA TCTAGAAGCC AAAGAAGGCG AAGTGCTAGA TCAGATGTCT 
CTGTTGGGGC GAGCTTCACA GTTAGAATCT GTAACACGGT CAAGAAATAG TTTGCCCAGC 
CAGGTTACTT TCCAAGATGT TGGAGAGCGA GACGCATCTG TTGGAGCACA CGCCAGTGAG 
GAGACAGTAA CATTTCGCCG CGAACGCAGC ACATTTAGGC GCCAAGCGGT TCGGCGCAGA 
CACAATGCAG GGAGTAATCC CACCCCTCCT GCGTCACTCA TCGGATCACC TCTGAGCCTT 
CAAGATGGTC ATCAAGGCCA GCAGTCATCA ACCCAGGTCA AAGTACAGTC CCGCCCCCCT 
TCCCAGGCTG CAGTGCTCAG CGCTAGCGCC TCCTTGCTGG TGAGAAATGG GAGTGTCCAC 
TTAGAAGCGT CTCATGACAA TGCATCTGCC GTAGGCAGTA GCAGTTTGCA CGATGAACTT 
GGTAAATTTA CCCCATCTCT ATATGAGACT GGAGGTTGTG ACATGTCACT TGTAAACTTT 
GAGCCAGCTG CAAGAAGGAC GTCTAATAAT CTTTGTGACA CAGATTCCCA TGTATCAAGT 
TCTACCTCAG TTCGGTTCTA CCCACATGAT CTACTCTCTC TTCCCCAGAT CCGATTAAAC 
AGGCTGCTAA CCATAGATAC AGATCTGTTA GAGCAGCAGG ATATTGATCT AAGCCCTGAC 
CTGCAGGACC AGATGCAACC ACAGGAACAG GCTGCCCAAA AAGTAAAACA CTACTACCGG 
TTTTGGATTC TCCCAAAGCT GTGGGTCGGC ATCAACTTTG ACAGGCTTAC ACTCTTAGCC 
TTGTTCGATA GGAACCGCGA GGTCCTAGAA AATGTGTTGT CTGTCATTCT CGCCATTCTA 
GTTGCCTTTT TGGGCTCTGT TCTTCTCATA GAAGGCTTCT TTAAAGATAT CTGGGTCTTC 
CAGTTTTGTC TTGTTATAGC CAGCTGTCAA TACTCTCTCC TTAAGAGTGT TCAACCAGAT 
TCTTCTTCCC CAAGACATGG CCATAATCGT ATCATTGCCT ATAGTAGGCC CGTTTACTTC 
TGTGCCTGTT GCGGCCTTAT TTGGTTATTG GATTATGGAA GCAAAAACAT GTCTACCACC 
AAATACAAAC TATATGGAAT GGCCTTCACC AATCCTCTGC TTCTAGTGTC AGCCAGGGAC 
TTAGTCATTG TCTTCACACT CTGTTTCCCA GTCGTGTTCT TTATTGGTCT CCTGCCTCAG 
GTGAACACAT TCGTCATGTA CCTGTGTGAG CAAGTAGATA TACATGTGTT TGGTGGAAAT 
GCTACCACCA GCTTGCTTGC AGCAATTTAC AGCTTTATCC GTAGCATTGT GGCCATAGCT 
TTACTCTACG GGCTGTGTTA CGGTGCTCTT AAGGATCCAT GGGATGCACA ACACATTCCT 
GTATTATTCT CTATTTTCTG TGGTGTTCTT GTGGCAGTTT CATACCATCT CAGCCGACAG 
AGCAGCGATC CCTCTGTCCT CATCTCACTG GTGCAATCAA AGATTTTTCC AAACAGCGAG 
GATACAAATC CTGAAGACCC ACTATCAGAA GTGAAAGACC CTCTTCCAGA AAAACTGAGA 
AGCTCTGTTA ATGAACGACT TCAGTCAGAC GTAGTAGTCT GTGTCGTTAT TGCAGTGTTG 
TACTTTGCAA TTCATGTCAG CACCGTATTT ACTGCATTAC AGCCGGTTCT GAGCTACGTA 
CTCTATGCAC TAGTTGGTTC TGTTGGACTC CTTACTCACT ACATTCTACC ACAAGTACGG 
AAACAGCTTC CTTGGCACTG CTTCTCCCAC CCGCTGCTGA AAACTAAGGA ATATTACCAA 
TTTGAAGTTC GAAATGCTGC TCATCTGATG TGGTTTGAGA AGGCTCACAT CTGGCTGCTT 
TTTATCGAGA AGAACATTAT TTATCCTCTT ATAGTTCTCA ATGAGCTTAG CATTGGTGCT 
AAGGATATCG TCAGCCCGAA AAAGCTGGAT GCAGAAGTTG GTGCATTGTT GATCACCATT 
GCCGGAATGA AGCTGCTGAG GTCGTCATTT AGCAGTCCCA CCTGCCAGTA TGCCACTGTC 
ATTTTCACTG TCCTCTTCTT TACTTTTGAC TACAAGGAGT ATACAGAAAA ACTGCTGCTA 
GATCTGTTCT TTATGTCAAT CCTCTTTAGC AAGCTCTGGG AACTGTTTTT CAAACTGCGC 
TTTGTCTACA CCTATATTGC CCCTTGGCAG ATCACGTGGG GATCAGCATT CCATGCTTTC 
GCACAGCCAT TTGCTGTACC TCATTCTGCA ATGCTGTTTG TACAGGCAAT TGTTTCTGCA 
CTGTTCTCCA CTCCCCTGAA CCCTTTCTTA GGGAGTGCAA TCTTTATTAC ATCATATGTA 
CGCCCTGTCA AGTTTTGGGA GAGAGACTAC AATACAAAGA GAGTTGACCA CTCAAACACA 
AGATTAGCCT CTCAGCTTGA CAGAAATCCA GGTTCAGATG ACAACAACTT GAATTCCATA 
TTTTATGAGC ATCTCACACG CTCCCTTCAG CATAGTCTGT GTGGTGATCT GTTGCTTGGC 
CGCTGGGGAA ATTACAATAC TGGGGATTGC TTTATTCTGG CCTCAGATTA TCTAAACGCC 
TTGGTGCACC TTATCGAAAT AGGCAATGGT CTGGTCACCT TCCAGCTAAG AGGACTAGAG 
TTCAGAGGTA CCTACTGCCA GCAGCGTGAA GTTGAAGCAA TCACTGAGGG TGTAGAGGAA 
GATGAAGGCT TTTGCTGTTG CGAACCTGGA CATATTCCTC ATATGCTGTC TTTCAATGCT 
GCCTTTGGGC AGCGCTGGCT GGCATGGGAA GTCTTGGTTA CAAAGTATGT TCTGGAAGGT 
TACAGCATCA CAGACAACAG TGCAGCCTCT ATGCTTCAAG TGTTTGACCT TCGCAGGATC 
CTCACTACCT ATTATGTTAA GGGCATTATA TACTATGTGA CAGCATCTGC AAAACTGGAG 
GAGTGGTTAG CAAATGAGAC AATGCAGGAA GGTCTTCGCC AGTGTTCTGA TCATTGTTAT 
GTTGATGTAG ATCCAACTTT CAACCCAAAT ATTGATGAAG ACTATGACCT TCGCTTAGCA 
GGCATTTCAC GGGAGAGCTT CTGTGCAAGC TATCTTAACT GGATTAAGTA CTGTTCCTCT 
AGAAGGGAGA AGCCTCTTGA TGCTGAAAAG GATTCACCTT TGGTAACACT TTGTTATGGG 
CTCTGTATTT TGGGGAGAAG GGCACTTGGT ACAGCTTCTC ATCACATGTC CAGCAATTTG 
GAGTCCTTTC TATATGGTTT ACATGCCTTG TTTAAAGGAG ATTTCCGCAT ATCATCTCTC 
CGTGATGAGT GGATTTTTGC TGATATGGAG CTTTTAAGAA AAGTAGTAGT TCCTGGCATA 
CGTATGTCAC TAAAACTTCA TCAGGACCAC TTTACATCTC CAGATGAGTA TGATGATCCT 
GCAGTTTTGT ATGAAGCCAT TACATCACAT GAGCAGAACC TTGTTATTGC GCATGAGGGA 
GATCCAGCGT GGAGGAGTGC TGTTCTTTCT AACTCACCTT CTCTTCTCGC GCTGCGTCAT 
GTGATGGATG ATGGAACCAA CGAATACAAG ATCATAATGT TAAATAAACG TTATCTTAGT 
TTTAGAGTCA TTAAGGTAAA CAAGGAGTGT GTGCGGGGCT TATGGGCTGG TCAGCAGCAA 
GAATTGGTCT TTCTTCGTAA TCGGAACCCT GAAAGAGGCA GCATCCAAAA TGCAAAGCAG 
GCTCTCAGGA ATATGATCAA TTCATCATGT GACCAACCTA TTGGATACCC AATTTATGTT 
TCTCCACTGA CAACCTCTTA TTCAGACAGC CATGATCAGC TTAAACAGAT ATTTGGTGGG 
CCAATTAGCT TGGCCAATAT CCGGAACTTC ATTGTATCTA CATGGCACAG ATTAAGAAAA 
GGTTGCGGTG CAGGATGCAA TAGTGGTGGA AATATTGAGG ACTCTGACAC TGGAGGCGGG 
ACCTCCATTA ATAGCAACAA TCCACCAAAT GCAAATGAAA CTCAGAGCAG TCTGTCACAA 
GTTGGTGTCC CAGGTGGAAC AGGGCATGTG CCGAATGCAC GGCTCCAGCC TCCGGGGATG 
CCTCATCCAT CAACACTAGG AACCAGTCAC AGTTCTCACT CCATGCAGTC AAGCCTTGTC 
AGACATTCCC CAGCTCGTGG ATCAGTAGCA AGTCAATCAT CTTACTGCTA CAACAGCCGT 
CACTCATCAC TTAGAACATC CACAACAGGA TTTGTGCCTT GTAGGCGCTC CTCTACCAGC 
CAGATATCTC TTCGCAATCT CCCCTCATCC ATTCAGTCCC GTCTTTCCAT GGTGAACCAA 
ACAGACCCCA CAGGACAGTC AGTTTTGGTT GGCCTTCCTT CCTCCAGCAG CTCAAGCCAG 
AGCATCCCTG CATGCAAAAG ACACACCTTA GTGGGCTTTT TAGGAACAGA AGGAGGCAAT 
AACCTCACTG AAACGCAATC CTGTGGCAAT TCTAGTCCAG CAACCCATTC AAGAGCCAAA 
AAGGAAGAGG TTGTTTACAG AGTCCAGATT GTGGATCCAA GCCAAGTGCT TGAAGGCGTG 
AATATATCCA AAAGAAAGGA GCTGCAGTGG CCAGATGAGC TGGTGCGGCT AAAAGCCGGC 
AAGAACAGCT GGAAAGACTG GAGCCCTCAG GAAGGCATGG AAGGCCATGT AATTCACCGC 
TGGGTGCCTT GCAGCAGAGA CCCTTGTAAT AGGTCCCACA TTGACAAAAC AATCCTGCTG 
ATTAAGATTG AGGACAAATA TGTTGCTGTG ATTGAAACTG GTGTGCTTGA ACTCGGAGCT 
GAAGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004917310.1
CDS	272..7240
Translation	MGSQTLQILRQGVWASITGGWYYDPHQSTFVNTLHLYIWLFLLCFPFTLYMALPTSLVIVAVYCPVVAAVFIVLKLVNYRLHRALDEGEIVERSASELTERVAKAEHSDSSARREDSNGPGDPGGGIEMSEFVREATPPVGCSSRNSYSGLDPSNQVGVSRYGGASGSKGDTDAAKTSDDISLSLGQSYSLCKEASEDQDLTVDQKLFRLVSNDSFVSMQPSISSGGKDLTRDFSDKGSIPTHSHHHHVDLSLSNACDTELASLVPLHSHSYRKDHRPRGVPRTSSSAVAFPDTSLTDFSLYQPRRGLDPVSELEASKPHSGSKDSLVHNACASGIFQLDCLKSKGAQQLSRSGSVKHMKTDKSMDSLRSLSTRSSGSTESYCSGTDRDTNSTVSSYKSEQTSSTHVESILSEHEDVTAAKRVHCSDLEEEHSSATDKRTSSIRTALERNTAQEAKDANTSDDVQSPRGLSSSASEEANKNPHANELTVQTDWLPGKLSVNIDEPGEKPVILVDLRSLKETGIKQRDNDVRPKSSSLIHRTSSAHKSCRRRTGKKRASSFDASRHKEYVSFQGIADTKPHSTGFCHDEDSSDQSDLSRTSSVQSAHRFSSDSSSSTTSHSCQSPEARYSALRTKYRIKERGTDSHHTHLNCEGTSKKRSNRRTSSASGTKSRARVLSLDSGTVVSLSDANRLAAPENIKTLTTSKSDLEAKEGEVLDQMSLLGRASQLESVTRSRNSLPSQVTFQDVGERDASVGAHASEETVTFRRERSTFRRQAVRRRHNAGSNPTPPASLIGSPLSLQDGHQGQQSSTQVKVQSRPPSQAAVLSASASLLVRNGSVHLEASHDNASAVGSSSLHDELGKFTPSLYETGGCDMSLVNFEPAARRTSNNLCDTDSHVSSSTSVRFYPHDLLSLPQIRLNRLLTIDTDLLEQQDIDLSPDLQDQMQPQEQAAQKVKHYYRFWILPKLWVGINFDRLTLLALFDRNREVLENVLSVILAILVAFLGSVLLIEGFFKDIWVFQFCLVIASCQYSLLKSVQPDSSSPRHGHNRIIAYSRPVYFCACCGLIWLLDYGSKNMSTTKYKLYGMAFTNPLLLVSARDLVIVFTLCFPVVFFIGLLPQVNTFVMYLCEQVDIHVFGGNATTSLLAAIYSFIRSIVAIALLYGLCYGALKDPWDAQHIPVLFSIFCGVLVAVSYHLSRQSSDPSVLISLVQSKIFPNSEDTNPEDPLSEVKDPLPEKLRSSVNERLQSDVVVCVVIAVLYFAIHVSTVFTALQPVLSYVLYALVGSVGLLTHYILPQVRKQLPWHCFSHPLLKTKEYYQFEVRNAAHLMWFEKAHIWLLFIEKNIIYPLIVLNELSIGAKDIVSPKKLDAEVGALLITIAGMKLLRSSFSSPTCQYATVIFTVLFFTFDYKEYTEKLLLDLFFMSILFSKLWELFFKLRFVYTYIAPWQITWGSAFHAFAQPFAVPHSAMLFVQAIVSALFSTPLNPFLGSAIFITSYVRPVKFWERDYNTKRVDHSNTRLASQLDRNPGSDDNNLNSIFYEHLTRSLQHSLCGDLLLGRWGNYNTGDCFILASDYLNALVHLIEIGNGLVTFQLRGLEFRGTYCQQREVEAITEGVEEDEGFCCCEPGHIPHMLSFNAAFGQRWLAWEVLVTKYVLEGYSITDNSAASMLQVFDLRRILTTYYVKGIIYYVTASAKLEEWLANETMQEGLRQCSDHCYVDVDPTFNPNIDEDYDLRLAGISRESFCASYLNWIKYCSSRREKPLDAEKDSPLVTLCYGLCILGRRALGTASHHMSSNLESFLYGLHALFKGDFRISSLRDEWIFADMELLRKVVVPGIRMSLKLHQDHFTSPDEYDDPAVLYEAITSHEQNLVIAHEGDPAWRSAVLSNSPSLLALRHVMDDGTNEYKIIMLNKRYLSFRVIKVNKECVRGLWAGQQQELVFLRNRNPERGSIQNAKQALRNMINSSCDQPIGYPIYVSPLTTSYSDSHDQLKQIFGGPISLANIRNFIVSTWHRLRKGCGAGCNSGGNIEDSDTGGGTSINSNNPPNANETQSSLSQVGVPGGTGHVPNARLQPPGMPHPSTLGTSHSSHSMQSSLVRHSPARGSVASQSSYCYNSRHSSLRTSTTGFVPCRRSSTSQISLRNLPSSIQSRLSMVNQTDPTGQSVLVGLPSSSSSSQSIPACKRHTLVGFLGTEGGNNLTETQSCGNSSPATHSRAKKEEVVYRVQIVDPSQVLEGVNISKRKELQWPDELVRLKAGKNSWKDWSPQEGMEGHVIHRWVPCSRDPCNRSHIDKTILLIKIEDKYVAVIETGVLELGAEV

Target ORF information:

RefSeq Version	XM_004917253.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pecanex homolog (pcnx), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004917253.3

ORF Insert Sequence:

ATGGGTTCCC AGACACTGCA AATCCTCCGG CAGGGAGTCT GGGCTTCCAT CACCGGAGGC 
TGGTACTACG ACCCCCACCA GAGTACCTTT GTCAATACCC TACACCTCTA CATCTGGCTC 
TTCCTCCTCT GCTTCCCCTT CACCCTCTAC ATGGCACTAC CCACATCTTT GGTGATTGTT 
GCTGTGTACT GCCCAGTCGT TGCAGCTGTT TTTATTGTTT TGAAGCTTGT TAACTACCGT 
CTGCACAGGG CATTGGATGA AGGAGAAATT GTGGAGAGGA GTGCTAGTGA GTTGACTGAG 
AGGGTGGCAA AGGCAGAACA CAGTGACAGC TCTGCAAGAA GAGAGGACAG CAATGGACCT 
GGTGATCCAG GTGGAGGGAT TGAAATGTCT GAATTTGTTC GAGAAGCAAC TCCTCCAGTT 
GGATGCAGTT CACGTAATTC CTACTCTGGG TTGGACCCAA GCAATCAGGT TGGTGTGTCT 
CGTTATGGAG GTGCATCTGG AAGCAAAGGG GATACAGATG CTGCAAAGAC ATCAGATGAT 
ATCAGTCTGA GTCTTGGCCA GAGTTACAGT TTGTGCAAAG AAGCAAGTGA AGACCAAGAT 
TTGACTGTGG ATCAGAAGCT TTTCCGTCTC GTCTCCAATG ACTCCTTTGT CTCCATGCAG 
CCATCTATAT CCTCGGGTGG AAAAGACTTA ACTAGAGACT TCAGTGACAA AGGATCAATC 
CCAACACACA GTCATCATCA CCATGTGGAT CTGTCTCTGT CTAATGCTTG TGACACAGAA 
TTGGCCTCAC TTGTTCCATT GCATTCACAT TCGTACAGGA AAGATCATCG ACCAAGAGGT 
GTCCCAAGGA CTTCAAGTTC TGCTGTTGCT TTCCCAGACA CTTCTCTCAC TGACTTTTCA 
CTCTATCAAC CAAGGCGCGG GCTGGATCCT GTTAGTGAAC TTGAAGCTTC CAAACCACAT 
TCTGGGTCCA AAGATTCACT AGTACATAAT GCTTGTGCTT CAGGGATTTT TCAATTAGAC 
TGTCTAAAAA GCAAAGGAGC CCAGCAGCTT TCAAGAAGTG GTAGTGTTAA ACACATGAAG 
ACTGACAAAA GCATGGATAG TTTAAGAAGC CTCAGCACGA GAAGCAGTGG ATCCACAGAA 
AGTTATTGTA GTGGAACAGA TCGGGACACA AATAGCACTG TTAGCAGCTA CAAAAGTGAA 
CAGACCAGTT CAACTCATGT TGAAAGTATT TTATCTGAGC ATGAGGATGT TACTGCTGCA 
AAGAGAGTCC ATTGTAGTGA CCTGGAGGAA GAACATAGCT CTGCTACTGA TAAAAGGACT 
AGTAGTATAA GAACTGCTTT AGAAAGAAAC ACTGCTCAGG AAGCCAAGGA TGCCAACACA 
TCTGATGATG TACAAAGTCC AAGGGGTCTT AGCTCATCAG CATCAGAGGA AGCTAATAAG 
AATCCACACG CAAATGAGTT AACTGTACAA ACAGACTGGC TTCCAGGTAA ACTGTCAGTG 
AACATTGATG AACCTGGGGA GAAACCAGTC ATTTTAGTTG ACTTGAGATC TTTGAAAGAA 
ACGGGTATTA AGCAGAGAGA CAATGACGTA CGACCCAAAT CATCTAGTTT AATACATCGA 
ACATCCTCTG CTCATAAATC ATGCAGGAGA CGGACAGGTA AAAAAAGAGC CAGCAGTTTT 
GATGCAAGTA GACACAAAGA ATATGTTTCC TTTCAAGGTA TAGCTGATAC CAAACCTCAC 
AGTACTGGCT TTTGTCATGA TGAAGATTCT AGCGACCAAA GCGACTTGAG CAGGACCTCA 
AGCGTTCAGT CGGCCCACCG TTTCAGCAGC GATAGCTCTT CCAGCACTAC TTCCCATTCA 
TGCCAGTCTC CTGAAGCAAG ATACAGTGCC CTAAGGACAA AATATCGAAT AAAAGAAAGA 
GGCACAGATT CACATCACAC ACACCTAAAT TGTGAAGGAA CTAGTAAAAA GCGGTCCAAC 
CGCAGGACTT CTAGTGCAAG TGGTACAAAA AGCCGAGCTA GAGTATTAAG TCTGGACAGT 
GGCACTGTAG TTAGTTTGAG TGATGCCAAC AGACTGGCCG CTCCTGAAAA TATAAAAACC 
TTAACCACTT CAAAATCAGA TCTAGAAGCC AAAGAAGGCG AAGTGCTAGA TCAGATGTCT 
CTGTTGGGGC GAGCTTCACA GTTAGAATCT GTAACACGGT CAAGAAATAG TTTGCCCAGC 
CAGGTTACTT TCCAAGATGT TGGAGAGCGA GACGCATCTG TTGGAGCACA CGCCAGTGAG 
GAGACAGTAA CATTTCGCCG CGAACGCAGC ACATTTAGGC GCCAAGCGGT TCGGCGCAGA 
CACAATGCAG GGAGTAATCC CACCCCTCCT GCGTCACTCA TCGGATCACC TCTGAGCCTT 
CAAGATGGTC ATCAAGGCCA GCAGTCATCA ACCCAGGTCA AAGTACAGTC CCGCCCCCCT 
TCCCAGGCTG CAGTGCTCAG CGCTAGCGCC TCCTTGCTGG TGAGAAATGG GAGTGTCCAC 
TTAGAAGCGT CTCATGACAA TGCATCTGCC GTAGGCAGTA GCAGTTTGCA CGATGAACTT 
GGTAAATTTA CCCCATCTCT ATATGAGACT GGAGGTTGTG ACATGTCACT TGTAAACTTT 
GAGCCAGCTG CAAGAAGGAC GTCTAATAAT CTTTGTGACA CAGATTCCCA TGTATCAAGT 
TCTACCTCAG TTCGGTTCTA CCCACATGAT CTACTCTCTC TTCCCCAGAT CCGATTAAAC 
AGGCTGCTAA CCATAGATAC AGATCTGTTA GAGCAGCAGG ATATTGATCT AAGCCCTGAC 
CTGCAGGACC AGATGCAACC ACAGGAACAG GCTGCCCAAA AAGTAAAACA CTACTACCGG 
TTTTGGATTC TCCCAAAGCT GTGGGTCGGC ATCAACTTTG ACAGGCTTAC ACTCTTAGCC 
TTGTTCGATA GGAACCGCGA GGTCCTAGAA AATGTGTTGT CTGTCATTCT CGCCATTCTA 
GTTGCCTTTT TGGGCTCTGT TCTTCTCATA GAAGGCTTCT TTAAAGATAT CTGGGTCTTC 
CAGTTTTGTC TTGTTATAGC CAGCTGTCAA TACTCTCTCC TTAAGAGTGT TCAACCAGAT 
TCTTCTTCCC CAAGACATGG CCATAATCGT ATCATTGCCT ATAGTAGGCC CGTTTACTTC 
TGTGCCTGTT GCGGCCTTAT TTGGTTATTG GATTATGGAA GCAAAAACAT GTCTACCACC 
AAATACAAAC TATATGGAAT GGCCTTCACC AATCCTCTGC TTCTAGTGTC AGCCAGGGAC 
TTAGTCATTG TCTTCACACT CTGTTTCCCA GTCGTGTTCT TTATTGGTCT CCTGCCTCAG 
GTGAACACAT TCGTCATGTA CCTGTGTGAG CAAGTAGATA TACATGTGTT TGGTGGAAAT 
GCTACCACCA GCTTGCTTGC AGCAATTTAC AGCTTTATCC GTAGCATTGT GGCCATAGCT 
TTACTCTACG GGCTGTGTTA CGGTGCTCTT AAGGATCCAT GGGATGCACA ACACATTCCT 
GTATTATTCT CTATTTTCTG TGGTGTTCTT GTGGCAGTTT CATACCATCT CAGCCGACAG 
AGCAGCGATC CCTCTGTCCT CATCTCACTG GTGCAATCAA AGATTTTTCC AAACAGCGAG 
GATACAAATC CTGAAGACCC ACTATCAGAA GTGAAAGACC CTCTTCCAGA AAAACTGAGA 
AGCTCTGTTA ATGAACGACT TCAGTCAGAC GTAGTAGTCT GTGTCGTTAT TGCAGTGTTG 
TACTTTGCAA TTCATGTCAG CACCGTATTT ACTGCATTAC AGCCGGTTCT GAGCTACGTA 
CTCTATGCAC TAGTTGGTTC TGTTGGACTC CTTACTCACT ACATTCTACC ACAAGTACGG 
AAACAGCTTC CTTGGCACTG CTTCTCCCAC CCGCTGCTGA AAACTAAGGA ATATTACCAA 
TTTGAAGTTC GAAATGCTGC TCATCTGATG TGGTTTGAGA AGGCTCACAT CTGGCTGCTT 
TTTATCGAGA AGAACATTAT TTATCCTCTT ATAGTTCTCA ATGAGCTTAG CATTGGTGCT 
AAGGATATCG TCAGCCCGAA AAAGCTGGAT GCAGAAGTTG GTGCATTGTT GATCACCATT 
GCCGGAATGA AGCTGCTGAG GTCGTCATTT AGCAGTCCCA CCTGCCAGTA TGCCACTGTC 
ATTTTCACTG TCCTCTTCTT TACTTTTGAC TACAAGGAGT ATACAGAAAA ACTGCTGCTA 
GATCTGTTCT TTATGTCAAT CCTCTTTAGC AAGCTCTGGG AACTGTTTTT CAAACTGCGC 
TTTGTCTACA CCTATATTGC CCCTTGGCAG ATCACGTGGG GATCAGCATT CCATGCTTTC 
GCACAGCCAT TTGCTGTACC TCATTCTGCA ATGCTGTTTG TACAGGCAAT TGTTTCTGCA 
CTGTTCTCCA CTCCCCTGAA CCCTTTCTTA GGGAGTGCAA TCTTTATTAC ATCATATGTA 
CGCCCTGTCA AGTTTTGGGA GAGAGACTAC AATACAAAGA GAGTTGACCA CTCAAACACA 
AGATTAGCCT CTCAGCTTGA CAGAAATCCA GGTTCAGATG ACAACAACTT GAATTCCATA 
TTTTATGAGC ATCTCACACG CTCCCTTCAG CATAGTCTGT GTGGTGATCT GTTGCTTGGC 
CGCTGGGGAA ATTACAATAC TGGGGATTGC TTTATTCTGG CCTCAGATTA TCTAAACGCC 
TTGGTGCACC TTATCGAAAT AGGCAATGGT CTGGTCACCT TCCAGCTAAG AGGACTAGAG 
TTCAGAGGTA CCTACTGCCA GCAGCGTGAA GTTGAAGCAA TCACTGAGGG TGTAGAGGAA 
GATGAAGGCT TTTGCTGTTG CGAACCTGGA CATATTCCTC ATATGCTGTC TTTCAATGCT 
GCCTTTGGGC AGCGCTGGCT GGCATGGGAA GTCTTGGTTA CAAAGTATGT TCTGGAAGGT 
TACAGCATCA CAGACAACAG TGCAGCCTCT ATGCTTCAAG TGTTTGACCT TCGCAGGATC 
CTCACTACCT ATTATGTTAA GGGCATTATA TACTATGTGA CAGCATCTGC AAAACTGGAG 
GAGTGGTTAG CAAATGAGAC AATGCAGGAA GGTCTTCGCC AGTGTTCTGA TCATTGTTAT 
GTTGATGTAG ATCCAACTTT CAACCCAAAT ATTGATGAAG ACTATGACCT TCGCTTAGCA 
GGCATTTCAC GGGAGAGCTT CTGTGCAAGC TATCTTAACT GGATTAAGTA CTGTTCCTCT 
AGAAGGGAGA AGCCTCTTGA TGCTGAAAAG GATTCACCTT TGGTAACACT TTGTTATGGG 
CTCTGTATTT TGGGGAGAAG GGCACTTGGT ACAGCTTCTC ATCACATGTC CAGCAATTTG 
GAGTCCTTTC TATATGGTTT ACATGCCTTG TTTAAAGGAG ATTTCCGCAT ATCATCTCTC 
CGTGATGAGT GGATTTTTGC TGATATGGAG CTTTTAAGAA AAGTAGTAGT TCCTGGCATA 
CGTATGTCAC TAAAACTTCA TCAGGACCAC TTTACATCTC CAGATGAGTA TGATGATCCT 
GCAGTTTTGT ATGAAGCCAT TACATCACAT GAGCAGAACC TTGTTATTGC GCATGAGGGA 
GATCCAGCGT GGAGGAGTGC TGTTCTTTCT AACTCACCTT CTCTTCTCGC GCTGCGTCAT 
GTGATGGATG ATGGAACCAA CGAATACAAG ATCATAATGT TAAATAAACG TTATCTTAGT 
TTTAGAGTCA TTAAGGTAAA CAAGGAGTGT GTGCGGGGCT TATGGGCTGG TCAGCAGCAA 
GAATTGGTCT TTCTTCGTAA TCGGAACCCT GAAAGAGGCA GCATCCAAAA TGCAAAGCAG 
GCTCTCAGGA ATATGATCAA TTCATCATGT GACCAACCTA TTGGATACCC AATTTATGTT 
TCTCCACTGA CAACCTCTTA TTCAGACAGC CATGATCAGC TTAAACAGAT ATTTGGTGGG 
CCAATTAGCT TGGCCAATAT CCGGAACTTC ATTGTATCTA CATGGCACAG ATTAAGAAAA 
GGTTGCGGTG CAGGATGCAA TAGTGGTGGA AATATTGAGG ACTCTGACAC TGGAGGCGGG 
ACCTCCATTA ATAGCAACAA TCCACCAAAT GCAAATGAAA CTCAGAGCAG TCTGTCACAA 
GTTGGTGTCC CAGGTGGAAC AGGGCATGTG CCGAATGCAC GGCTCCAGCC TCCGGGGATG 
CCTCATCCAT CAACACTAGG AACCAGTCAC AGTTCTCACT CCATGCAGTC AAGCCTTGTC 
AGACATTCCC CAGCTCGTGG ATCAGTAGCA AGTCAATCAT CTTACTGCTA CAACAGCCGT 
CACTCATCAC TTAGAACATC CACAACAGGA TTTGTGCCTT GTAGGCGCTC CTCTACCAGC 
CAGATATCTC TTCGCAATCT CCCCTCATCC ATTCAGTCCC GTCTTTCCAT GGTGAACCAA 
ACAGACCCCA CAGGACAGTC AGTTTTGGTT GGCCTTCCTT CCTCCAGCAG CTCAAGCCAG 
AGCATCCCTG CATGCAAAAG ACACACCTTA GTGGGCTTTT TAGGAACAGA AGGAGGCAAT 
AACCTCACTG AAACGCAATC CTGTGGCAAT TCTAGTCCAG CAACCCATTC AAGAGCCAAA 
AAGGAAGAGG TTGTTTACAG AGTCCAGATT GTGGATCCAA GCCAAGTGCT TGAAGGCGTG 
AATATATCCA AAAGAAAGGA GCTGCAGTGG CCAGATGAGC TGGTGCGGCT AAAAGCCGGC 
AAGAACAGCT GGAAAGACTG GAGCCCTCAG GAAGGCATGG AAGGCCATGT AATTCACCGC 
TGGGTGCCTT GCAGCAGAGA CCCTTGTAAT AGGTCCCACA TTGACAAAAC AATCCTGCTG 
ATTAAGATTG AGGACAAATA TGTTGCTGTG ATTGAAACTG GTGTGCTTGA ACTCGGAGCT 
GAAGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pcnx1 ( XM_004917255.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004917312.1 Xenopus tropicalis pecanex homolog (pcnx), transcript variant X4, mRNA.

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CloneID	OXa15007
Clone ID Related Accession (Same CDS sequence)	XM_004917255.3
Accession Version	XM_004917255.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6936bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pecanex-like protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004917255.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCC AGACACTGCA AATCCTCCGG CAGGGAGTCT GGGCTTCCAT CACCGGAGGC 
TGGTACTACG ACCCCCACCA GAGTACCTTT GTCAATACCC TACACCTCTA CATCTGGCTC 
TTCCTCCTCT GCTTCCCCTT CACCCTCTAC ATGGCACTAC CCACATCTTT GGTGATTGTT 
GCTGTGTACT GCCCAGTCGT TGCAGCTGTT TTTATTGTTT TGAAGCTTGT TAACTACCGT 
CTGCACAGGG CATTGGATGA AGGAGAAATT GTGGAGAGGA GTGCTAGTGA GTTGACTGAG 
AGGGTGGCAA AGGCAGAACA CAGTGACAGC TCTGCAAGAA GAGAGGACAG CAATGGACCT 
GGTGATCCAG GTGGAGGGAT TGAAATGTCT GAATTTGTTC GAGAAGCAAC TCCTCCAGTT 
GGATGCAGTT CACGTAATTC CTACTCTGGG TTGGACCCAA GCAATCAGGT TGGTGTGTCT 
CGTTATGGAG GTGCATCTGG AAGCAAAGAT TTGACTGTGG ATCAGAAGCT TTTCCGTCTC 
GTCTCCAATG ACTCCTTTGT CTCCATGCAG CCATCTATAT CCTCGGGTGG AAAAGACTTA 
ACTAGAGACT TCAGTGACAA AGGATCAATC CCAACACACA GTCATCATCA CCATGTGGAT 
CTGTCTCTGT CTAATGCTTG TGACACAGAA TTGGCCTCAC TTGTTCCATT GCATTCACAT 
TCGTACAGGA AAGATCATCG ACCAAGAGGT GTCCCAAGGA CTTCAAGTTC TGCTGTTGCT 
TTCCCAGACA CTTCTCTCAC TGACTTTTCA CTCTATCAAC CAAGGCGCGG GCTGGATCCT 
GTTAGTGAAC TTGAAGCTTC CAAACCACAT TCTGGGTCCA AAGATTCACT AGTACATAAT 
GCTTGTGCTT CAGGGATTTT TCAATTAGAC TGTCTAAAAA GCAAAGGAGC CCAGCAGCTT 
TCAAGAAGTG GTAGTGTTAA ACACATGAAG ACTGACAAAA GCATGGATAG TTTAAGAAGC 
CTCAGCACGA GAAGCAGTGG ATCCACAGAA AGTTATTGTA GTGGAACAGA TCGGGACACA 
AATAGCACTG TTAGCAGCTA CAAAAGTGAA CAGACCAGTT CAACTCATGT TGAAAGTATT 
TTATCTGAGC ATGAGGATGT TACTGCTGCA AAGAGAGTCC ATTGTAGTGA CCTGGAGGAA 
GAACATAGCT CTGCTACTGA TAAAAGGACT AGTAGTATAA GAACTGCTTT AGAAAGAAAC 
ACTGCTCAGG AAGCCAAGGA TGCCAACACA TCTGATGATG TACAAAGTCC AAGGGGTCTT 
AGCTCATCAG CATCAGAGGA AGCTAATAAG AATCCACACG CAAATGAGTT AACTGTACAA 
ACAGACTGGC TTCCAGGTAA ACTGTCAGTG AACATTGATG AACCTGGGGA GAAACCAGTC 
ATTTTAGTTG ACTTGAGATC TTTGAAAGAA ACGGGTATTA AGCAGAGAGA CAATGACGTA 
CGACCCAAAT CATCTAGTTT AATACATCGA ACATCCTCTG CTCATAAATC ATGCAGGAGA 
CGGACAGGTA AAAAAAGAGC CAGCAGTTTT GATGCAAGTA GACACAAAGA ATATGTTTCC 
TTTCAAGGTA TAGCTGATAC CAAACCTCAC AGTACTGGCT TTTGTCATGA TGAAGATTCT 
AGCGACCAAA GCGACTTGAG CAGGACCTCA AGCGTTCAGT CGGCCCACCG TTTCAGCAGC 
GATAGCTCTT CCAGCACTAC TTCCCATTCA TGCCAGTCTC CTGAAGCAAG ATACAGTGCC 
CTAAGGACAA AATATCGAAT AAAAGAAAGA GGCACAGATT CACATCACAC ACACCTAAAT 
TGTGAAGGAA CTAGTAAAAA GCGGTCCAAC CGCAGGACTT CTAGTGCAAG TGGTACAAAA 
AGCCGAGCTA GAGTATTAAG TCTGGACAGT GGCACTGTAG TTAGTTTGAG TGATGCCAAC 
AGACTGGCCG CTCCTGAAAA TATAAAAACC TTAACCACTT CAAAATCAGA TCTAGAAGCC 
AAAGAAGGCG AAGTGCTAGA TCAGATGTCT CTGTTGGGGC GAGCTTCACA GTTAGAATCT 
GTAACACGGT CAAGAAATAG TTTGCCCAGC CAGGTTACTT TCCAAGATGT TGGAGAGCGA 
GACGCATCTG TTGGAGCACA CGCCAGTGAG GAGACAGTAA CATTTCGCCG CGAACGCAGC 
ACATTTAGGC GCCAAGCGGT TCGGCGCAGA CACAATGCAG GGAGTAATCC CACCCCTCCT 
GCGTCACTCA TCGGATCACC TCTGAGCCTT CAAGATGGTC ATCAAGGCCA GCAGTCATCA 
ACCCAGGTCA AAGTACAGTC CCGCCCCCCT TCCCAGGCTG CAGTGCTCAG CGCTAGCGCC 
TCCTTGCTGG TGAGAAATGG GAGTGTCCAC TTAGAAGCGT CTCATGACAA TGCATCTGCC 
GTAGGCAGTA GCAGTTTGCA CGATGAACTT GGTATGCAGG CCTTATGTAA CCTTGGTGAC 
ATGGAAGTTT TAATGCAGCA CATTTTGGGT AAATTTACCC CATCTCTATA TGAGACTGGA 
GGTTGTGACA TGTCACTTGT AAACTTTGAG CCAGCTGCAA GAAGGACGTC TAATAATCTT 
TGTGACACAG ATTCCCATGT ATCAAGTTCT ACCTCAGTTC GGTTCTACCC ACATGATCTA 
CTCTCTCTTC CCCAGATCCG ATTAAACAGG CTGCTAACCA TAGATACAGA TCTGTTAGAG 
CAGCAGGATA TTGATCTAAG CCCTGACCTG CAGGACCAGA TGCAACCACA GGAACAGGCT 
GCCCAAAAAG TAAAACACTA CTACCGGTTT TGGATTCTCC CAAAGCTGTG GGTCGGCATC 
AACTTTGACA GGCTTACACT CTTAGCCTTG TTCGATAGGA ACCGCGAGGT CCTAGAAAAT 
GTGTTGTCTG TCATTCTCGC CATTCTAGTT GCCTTTTTGG GCTCTGTTCT TCTCATAGAA 
GGCTTCTTTA AAGATATCTG GGTCTTCCAG TTTTGTCTTG TTATAGCCAG CTGTCAATAC 
TCTCTCCTTA AGAGTGTTCA ACCAGATTCT TCTTCCCCAA GACATGGCCA TAATCGTATC 
ATTGCCTATA GTAGGCCCGT TTACTTCTGT GCCTGTTGCG GCCTTATTTG GTTATTGGAT 
TATGGAAGCA AAAACATGTC TACCACCAAA TACAAACTAT ATGGAATGGC CTTCACCAAT 
CCTCTGCTTC TAGTGTCAGC CAGGGACTTA GTCATTGTCT TCACACTCTG TTTCCCAGTC 
GTGTTCTTTA TTGGTCTCCT GCCTCAGGTG AACACATTCG TCATGTACCT GTGTGAGCAA 
GTAGATATAC ATGTGTTTGG TGGAAATGCT ACCACCAGCT TGCTTGCAGC AATTTACAGC 
TTTATCCGTA GCATTGTGGC CATAGCTTTA CTCTACGGGC TGTGTTACGG TGCTCTTAAG 
GATCCATGGG ATGCACAACA CATTCCTGTA TTATTCTCTA TTTTCTGTGG TGTTCTTGTG 
GCAGTTTCAT ACCATCTCAG CCGACAGAGC AGCGATCCCT CTGTCCTCAT CTCACTGGTG 
CAATCAAAGA TTTTTCCAAA CAGCGAGGAT ACAAATCCTG AAGACCCACT ATCAGAAGTG 
AAAGACCCTC TTCCAGAAAA ACTGAGAAGC TCTGTTAATG AACGACTTCA GTCAGACGTA 
GTAGTCTGTG TCGTTATTGC AGTGTTGTAC TTTGCAATTC ATGTCAGCAC CGTATTTACT 
GCATTACAGC CGGTTCTGAG CTACGTACTC TATGCACTAG TTGGTTCTGT TGGACTCCTT 
ACTCACTACA TTCTACCACA AGTACGGAAA CAGCTTCCTT GGCACTGCTT CTCCCACCCG 
CTGCTGAAAA CTAAGGAATA TTACCAATTT GAAGTTCGAA ATGCTGCTCA TCTGATGTGG 
TTTGAGAAGG CTCACATCTG GCTGCTTTTT ATCGAGAAGA ACATTATTTA TCCTCTTATA 
GTTCTCAATG AGCTTAGCAT TGGTGCTAAG GATATCGTCA GCCCGAAAAA GCTGGATGCA 
GAAGTTGGTG CATTGTTGAT CACCATTGCC GGAATGAAGC TGCTGAGGTC GTCATTTAGC 
AGTCCCACCT GCCAGTATGC CACTGTCATT TTCACTGTCC TCTTCTTTAC TTTTGACTAC 
AAGGAGTATA CAGAAAAACT GCTGCTAGAT CTGTTCTTTA TGTCAATCCT CTTTAGCAAG 
CTCTGGGAAC TGTTTTTCAA ACTGCGCTTT GTCTACACCT ATATTGCCCC TTGGCAGATC 
ACGTGGGGAT CAGCATTCCA TGCTTTCGCA CAGCCATTTG CTGTACCTCA TTCTGCAATG 
CTGTTTGTAC AGGCAATTGT TTCTGCACTG TTCTCCACTC CCCTGAACCC TTTCTTAGGG 
AGTGCAATCT TTATTACATC ATATGTACGC CCTGTCAAGT TTTGGGAGAG AGACTACAAT 
ACAAAGAGAG TTGACCACTC AAACACAAGA TTAGCCTCTC AGCTTGACAG AAATCCAGGT 
TCAGATGACA ACAACTTGAA TTCCATATTT TATGAGCATC TCACACGCTC CCTTCAGCAT 
AGTCTGTGTG GTGATCTGTT GCTTGGCCGC TGGGGAAATT ACAATACTGG GGATTGCTTT 
ATTCTGGCCT CAGATTATCT AAACGCCTTG GTGCACCTTA TCGAAATAGG CAATGGTCTG 
GTCACCTTCC AGCTAAGAGG ACTAGAGTTC AGAGGTACCT ACTGCCAGCA GCGTGAAGTT 
GAAGCAATCA CTGAGGGTGT AGAGGAAGAT GAAGGCTTTT GCTGTTGCGA ACCTGGACAT 
ATTCCTCATA TGCTGTCTTT CAATGCTGCC TTTGGGCAGC GCTGGCTGGC ATGGGAAGTC 
TTGGTTACAA AGTATGTTCT GGAAGGTTAC AGCATCACAG ACAACAGTGC AGCCTCTATG 
CTTCAAGTGT TTGACCTTCG CAGGATCCTC ACTACCTATT ATGTTAAGGG CATTATATAC 
TATGTGACAG CATCTGCAAA ACTGGAGGAG TGGTTAGCAA ATGAGACAAT GCAGGAAGGT 
CTTCGCCAGT GTTCTGATCA TTGTTATGTT GATGTAGATC CAACTTTCAA CCCAAATATT 
GATGAAGACT ATGACCTTCG CTTAGCAGGC ATTTCACGGG AGAGCTTCTG TGCAAGCTAT 
CTTAACTGGA TTAAGTACTG TTCCTCTAGA AGGGAGAAGC CTCTTGATGC TGAAAAGGAT 
TCACCTTTGG TAACACTTTG TTATGGGCTC TGTATTTTGG GGAGAAGGGC ACTTGGTACA 
GCTTCTCATC ACATGTCCAG CAATTTGGAG TCCTTTCTAT ATGGTTTACA TGCCTTGTTT 
AAAGGAGATT TCCGCATATC ATCTCTCCGT GATGAGTGGA TTTTTGCTGA TATGGAGCTT 
TTAAGAAAAG TAGTAGTTCC TGGCATACGT ATGTCACTAA AACTTCATCA GGACCACTTT 
ACATCTCCAG ATGAGTATGA TGATCCTGCA GTTTTGTATG AAGCCATTAC ATCACATGAG 
CAGAACCTTG TTATTGCGCA TGAGGGAGAT CCAGCGTGGA GGAGTGCTGT TCTTTCTAAC 
TCACCTTCTC TTCTCGCGCT GCGTCATGTG ATGGATGATG GAACCAACGA ATACAAGATC 
ATAATGTTAA ATAAACGTTA TCTTAGTTTT AGAGTCATTA AGGTAAACAA GGAGTGTGTG 
CGGGGCTTAT GGGCTGGTCA GCAGCAAGAA TTGGTCTTTC TTCGTAATCG GAACCCTGAA 
AGAGGCAGCA TCCAAAATGC AAAGCAGGCT CTCAGGAATA TGATCAATTC ATCATGTGAC 
CAACCTATTG GATACCCAAT TTATGTTTCT CCACTGACAA CCTCTTATTC AGACAGCCAT 
GATCAGCTTA AACAGATATT TGGTGGGCCA ATTAGCTTGG CCAATATCCG GAACTTCATT 
GTATCTACAT GGCACAGATT AAGAAAAGGT TGCGGTGCAG GATGCAATAG TGGTGGAAAT 
ATTGAGGACT CTGACACTGG AGGCGGGACC TCCATTAATA GCAACAATCC ACCAAATGCA 
AATGAAACTC AGAGCAGTCT GTCACAAGTT GGTGTCCCAG GTGGAACAGG GCATGTGCCG 
AATGCACGGC TCCAGCCTCC GGGGATGCCT CATCCATCAA CACTAGGAAC CAGTCACAGT 
TCTCACTCCA TGCAGTCAAG CCTTGTCAGA CATTCCCCAG CTCGTGGATC AGTAGCAAGT 
CAATCATCTT ACTGCTACAA CAGCCGTCAC TCATCACTTA GAACATCCAC AACAGGATTT 
GTGCCTTGTA GGCGCTCCTC TACCAGCCAG ATATCTCTTC GCAATCTCCC CTCATCCATT 
CAGTCCCGTC TTTCCATGGT GAACCAAACA GACCCCACAG GACAGTCAGT TTTGGTTGGC 
CTTCCTTCCT CCAGCAGCTC AAGCCAGAGC ATCCCTGCAT GCAAAAGACA CACCTTAGTG 
GGCTTTTTAG GAACAGAAGG AGGCAATAAC CTCACTGAAA CGCAATCCTG TGGCAATTCT 
AGTCCAGCAA CCCATTCAAG AGCCAAAAAG GAAGAGGTTG TTTACAGAGT CCAGATTGTG 
GATCCAAGCC AAGTGCTTGA AGGCGTGAAT ATATCCAAAA GAAAGGAGCT GCAGTGGCCA 
GATGAGCTGG TGCGGCTAAA AGCCGGCAAG AACAGCTGGA AAGACTGGAG CCCTCAGGAA 
GGCATGGAAG GCCATGTAAT TCACCGCTGG GTGCCTTGCA GCAGAGACCC TTGTAATAGG 
TCCCACATTG ACAAAACAAT CCTGCTGATT AAGATTGAGG ACAAATATGT TGCTGTGATT 
GAAACTGGTG TGCTTGAACT CGGAGCTGAA GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004917312.1
CDS	272..7207
Translation	MGSQTLQILRQGVWASITGGWYYDPHQSTFVNTLHLYIWLFLLCFPFTLYMALPTSLVIVAVYCPVVAAVFIVLKLVNYRLHRALDEGEIVERSASELTERVAKAEHSDSSARREDSNGPGDPGGGIEMSEFVREATPPVGCSSRNSYSGLDPSNQVGVSRYGGASGSKDLTVDQKLFRLVSNDSFVSMQPSISSGGKDLTRDFSDKGSIPTHSHHHHVDLSLSNACDTELASLVPLHSHSYRKDHRPRGVPRTSSSAVAFPDTSLTDFSLYQPRRGLDPVSELEASKPHSGSKDSLVHNACASGIFQLDCLKSKGAQQLSRSGSVKHMKTDKSMDSLRSLSTRSSGSTESYCSGTDRDTNSTVSSYKSEQTSSTHVESILSEHEDVTAAKRVHCSDLEEEHSSATDKRTSSIRTALERNTAQEAKDANTSDDVQSPRGLSSSASEEANKNPHANELTVQTDWLPGKLSVNIDEPGEKPVILVDLRSLKETGIKQRDNDVRPKSSSLIHRTSSAHKSCRRRTGKKRASSFDASRHKEYVSFQGIADTKPHSTGFCHDEDSSDQSDLSRTSSVQSAHRFSSDSSSSTTSHSCQSPEARYSALRTKYRIKERGTDSHHTHLNCEGTSKKRSNRRTSSASGTKSRARVLSLDSGTVVSLSDANRLAAPENIKTLTTSKSDLEAKEGEVLDQMSLLGRASQLESVTRSRNSLPSQVTFQDVGERDASVGAHASEETVTFRRERSTFRRQAVRRRHNAGSNPTPPASLIGSPLSLQDGHQGQQSSTQVKVQSRPPSQAAVLSASASLLVRNGSVHLEASHDNASAVGSSSLHDELGMQALCNLGDMEVLMQHILGKFTPSLYETGGCDMSLVNFEPAARRTSNNLCDTDSHVSSSTSVRFYPHDLLSLPQIRLNRLLTIDTDLLEQQDIDLSPDLQDQMQPQEQAAQKVKHYYRFWILPKLWVGINFDRLTLLALFDRNREVLENVLSVILAILVAFLGSVLLIEGFFKDIWVFQFCLVIASCQYSLLKSVQPDSSSPRHGHNRIIAYSRPVYFCACCGLIWLLDYGSKNMSTTKYKLYGMAFTNPLLLVSARDLVIVFTLCFPVVFFIGLLPQVNTFVMYLCEQVDIHVFGGNATTSLLAAIYSFIRSIVAIALLYGLCYGALKDPWDAQHIPVLFSIFCGVLVAVSYHLSRQSSDPSVLISLVQSKIFPNSEDTNPEDPLSEVKDPLPEKLRSSVNERLQSDVVVCVVIAVLYFAIHVSTVFTALQPVLSYVLYALVGSVGLLTHYILPQVRKQLPWHCFSHPLLKTKEYYQFEVRNAAHLMWFEKAHIWLLFIEKNIIYPLIVLNELSIGAKDIVSPKKLDAEVGALLITIAGMKLLRSSFSSPTCQYATVIFTVLFFTFDYKEYTEKLLLDLFFMSILFSKLWELFFKLRFVYTYIAPWQITWGSAFHAFAQPFAVPHSAMLFVQAIVSALFSTPLNPFLGSAIFITSYVRPVKFWERDYNTKRVDHSNTRLASQLDRNPGSDDNNLNSIFYEHLTRSLQHSLCGDLLLGRWGNYNTGDCFILASDYLNALVHLIEIGNGLVTFQLRGLEFRGTYCQQREVEAITEGVEEDEGFCCCEPGHIPHMLSFNAAFGQRWLAWEVLVTKYVLEGYSITDNSAASMLQVFDLRRILTTYYVKGIIYYVTASAKLEEWLANETMQEGLRQCSDHCYVDVDPTFNPNIDEDYDLRLAGISRESFCASYLNWIKYCSSRREKPLDAEKDSPLVTLCYGLCILGRRALGTASHHMSSNLESFLYGLHALFKGDFRISSLRDEWIFADMELLRKVVVPGIRMSLKLHQDHFTSPDEYDDPAVLYEAITSHEQNLVIAHEGDPAWRSAVLSNSPSLLALRHVMDDGTNEYKIIMLNKRYLSFRVIKVNKECVRGLWAGQQQELVFLRNRNPERGSIQNAKQALRNMINSSCDQPIGYPIYVSPLTTSYSDSHDQLKQIFGGPISLANIRNFIVSTWHRLRKGCGAGCNSGGNIEDSDTGGGTSINSNNPPNANETQSSLSQVGVPGGTGHVPNARLQPPGMPHPSTLGTSHSSHSMQSSLVRHSPARGSVASQSSYCYNSRHSSLRTSTTGFVPCRRSSTSQISLRNLPSSIQSRLSMVNQTDPTGQSVLVGLPSSSSSSQSIPACKRHTLVGFLGTEGGNNLTETQSCGNSSPATHSRAKKEEVVYRVQIVDPSQVLEGVNISKRKELQWPDELVRLKAGKNSWKDWSPQEGMEGHVIHRWVPCSRDPCNRSHIDKTILLIKIEDKYVAVIETGVLELGAEV

Target ORF information:

RefSeq Version	XM_004917255.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pecanex homolog (pcnx), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004917255.3

ORF Insert Sequence:

ATGGGTTCCC AGACACTGCA AATCCTCCGG CAGGGAGTCT GGGCTTCCAT CACCGGAGGC 
TGGTACTACG ACCCCCACCA GAGTACCTTT GTCAATACCC TACACCTCTA CATCTGGCTC 
TTCCTCCTCT GCTTCCCCTT CACCCTCTAC ATGGCACTAC CCACATCTTT GGTGATTGTT 
GCTGTGTACT GCCCAGTCGT TGCAGCTGTT TTTATTGTTT TGAAGCTTGT TAACTACCGT 
CTGCACAGGG CATTGGATGA AGGAGAAATT GTGGAGAGGA GTGCTAGTGA GTTGACTGAG 
AGGGTGGCAA AGGCAGAACA CAGTGACAGC TCTGCAAGAA GAGAGGACAG CAATGGACCT 
GGTGATCCAG GTGGAGGGAT TGAAATGTCT GAATTTGTTC GAGAAGCAAC TCCTCCAGTT 
GGATGCAGTT CACGTAATTC CTACTCTGGG TTGGACCCAA GCAATCAGGT TGGTGTGTCT 
CGTTATGGAG GTGCATCTGG AAGCAAAGAT TTGACTGTGG ATCAGAAGCT TTTCCGTCTC 
GTCTCCAATG ACTCCTTTGT CTCCATGCAG CCATCTATAT CCTCGGGTGG AAAAGACTTA 
ACTAGAGACT TCAGTGACAA AGGATCAATC CCAACACACA GTCATCATCA CCATGTGGAT 
CTGTCTCTGT CTAATGCTTG TGACACAGAA TTGGCCTCAC TTGTTCCATT GCATTCACAT 
TCGTACAGGA AAGATCATCG ACCAAGAGGT GTCCCAAGGA CTTCAAGTTC TGCTGTTGCT 
TTCCCAGACA CTTCTCTCAC TGACTTTTCA CTCTATCAAC CAAGGCGCGG GCTGGATCCT 
GTTAGTGAAC TTGAAGCTTC CAAACCACAT TCTGGGTCCA AAGATTCACT AGTACATAAT 
GCTTGTGCTT CAGGGATTTT TCAATTAGAC TGTCTAAAAA GCAAAGGAGC CCAGCAGCTT 
TCAAGAAGTG GTAGTGTTAA ACACATGAAG ACTGACAAAA GCATGGATAG TTTAAGAAGC 
CTCAGCACGA GAAGCAGTGG ATCCACAGAA AGTTATTGTA GTGGAACAGA TCGGGACACA 
AATAGCACTG TTAGCAGCTA CAAAAGTGAA CAGACCAGTT CAACTCATGT TGAAAGTATT 
TTATCTGAGC ATGAGGATGT TACTGCTGCA AAGAGAGTCC ATTGTAGTGA CCTGGAGGAA 
GAACATAGCT CTGCTACTGA TAAAAGGACT AGTAGTATAA GAACTGCTTT AGAAAGAAAC 
ACTGCTCAGG AAGCCAAGGA TGCCAACACA TCTGATGATG TACAAAGTCC AAGGGGTCTT 
AGCTCATCAG CATCAGAGGA AGCTAATAAG AATCCACACG CAAATGAGTT AACTGTACAA 
ACAGACTGGC TTCCAGGTAA ACTGTCAGTG AACATTGATG AACCTGGGGA GAAACCAGTC 
ATTTTAGTTG ACTTGAGATC TTTGAAAGAA ACGGGTATTA AGCAGAGAGA CAATGACGTA 
CGACCCAAAT CATCTAGTTT AATACATCGA ACATCCTCTG CTCATAAATC ATGCAGGAGA 
CGGACAGGTA AAAAAAGAGC CAGCAGTTTT GATGCAAGTA GACACAAAGA ATATGTTTCC 
TTTCAAGGTA TAGCTGATAC CAAACCTCAC AGTACTGGCT TTTGTCATGA TGAAGATTCT 
AGCGACCAAA GCGACTTGAG CAGGACCTCA AGCGTTCAGT CGGCCCACCG TTTCAGCAGC 
GATAGCTCTT CCAGCACTAC TTCCCATTCA TGCCAGTCTC CTGAAGCAAG ATACAGTGCC 
CTAAGGACAA AATATCGAAT AAAAGAAAGA GGCACAGATT CACATCACAC ACACCTAAAT 
TGTGAAGGAA CTAGTAAAAA GCGGTCCAAC CGCAGGACTT CTAGTGCAAG TGGTACAAAA 
AGCCGAGCTA GAGTATTAAG TCTGGACAGT GGCACTGTAG TTAGTTTGAG TGATGCCAAC 
AGACTGGCCG CTCCTGAAAA TATAAAAACC TTAACCACTT CAAAATCAGA TCTAGAAGCC 
AAAGAAGGCG AAGTGCTAGA TCAGATGTCT CTGTTGGGGC GAGCTTCACA GTTAGAATCT 
GTAACACGGT CAAGAAATAG TTTGCCCAGC CAGGTTACTT TCCAAGATGT TGGAGAGCGA 
GACGCATCTG TTGGAGCACA CGCCAGTGAG GAGACAGTAA CATTTCGCCG CGAACGCAGC 
ACATTTAGGC GCCAAGCGGT TCGGCGCAGA CACAATGCAG GGAGTAATCC CACCCCTCCT 
GCGTCACTCA TCGGATCACC TCTGAGCCTT CAAGATGGTC ATCAAGGCCA GCAGTCATCA 
ACCCAGGTCA AAGTACAGTC CCGCCCCCCT TCCCAGGCTG CAGTGCTCAG CGCTAGCGCC 
TCCTTGCTGG TGAGAAATGG GAGTGTCCAC TTAGAAGCGT CTCATGACAA TGCATCTGCC 
GTAGGCAGTA GCAGTTTGCA CGATGAACTT GGTATGCAGG CCTTATGTAA CCTTGGTGAC 
ATGGAAGTTT TAATGCAGCA CATTTTGGGT AAATTTACCC CATCTCTATA TGAGACTGGA 
GGTTGTGACA TGTCACTTGT AAACTTTGAG CCAGCTGCAA GAAGGACGTC TAATAATCTT 
TGTGACACAG ATTCCCATGT ATCAAGTTCT ACCTCAGTTC GGTTCTACCC ACATGATCTA 
CTCTCTCTTC CCCAGATCCG ATTAAACAGG CTGCTAACCA TAGATACAGA TCTGTTAGAG 
CAGCAGGATA TTGATCTAAG CCCTGACCTG CAGGACCAGA TGCAACCACA GGAACAGGCT 
GCCCAAAAAG TAAAACACTA CTACCGGTTT TGGATTCTCC CAAAGCTGTG GGTCGGCATC 
AACTTTGACA GGCTTACACT CTTAGCCTTG TTCGATAGGA ACCGCGAGGT CCTAGAAAAT 
GTGTTGTCTG TCATTCTCGC CATTCTAGTT GCCTTTTTGG GCTCTGTTCT TCTCATAGAA 
GGCTTCTTTA AAGATATCTG GGTCTTCCAG TTTTGTCTTG TTATAGCCAG CTGTCAATAC 
TCTCTCCTTA AGAGTGTTCA ACCAGATTCT TCTTCCCCAA GACATGGCCA TAATCGTATC 
ATTGCCTATA GTAGGCCCGT TTACTTCTGT GCCTGTTGCG GCCTTATTTG GTTATTGGAT 
TATGGAAGCA AAAACATGTC TACCACCAAA TACAAACTAT ATGGAATGGC CTTCACCAAT 
CCTCTGCTTC TAGTGTCAGC CAGGGACTTA GTCATTGTCT TCACACTCTG TTTCCCAGTC 
GTGTTCTTTA TTGGTCTCCT GCCTCAGGTG AACACATTCG TCATGTACCT GTGTGAGCAA 
GTAGATATAC ATGTGTTTGG TGGAAATGCT ACCACCAGCT TGCTTGCAGC AATTTACAGC 
TTTATCCGTA GCATTGTGGC CATAGCTTTA CTCTACGGGC TGTGTTACGG TGCTCTTAAG 
GATCCATGGG ATGCACAACA CATTCCTGTA TTATTCTCTA TTTTCTGTGG TGTTCTTGTG 
GCAGTTTCAT ACCATCTCAG CCGACAGAGC AGCGATCCCT CTGTCCTCAT CTCACTGGTG 
CAATCAAAGA TTTTTCCAAA CAGCGAGGAT ACAAATCCTG AAGACCCACT ATCAGAAGTG 
AAAGACCCTC TTCCAGAAAA ACTGAGAAGC TCTGTTAATG AACGACTTCA GTCAGACGTA 
GTAGTCTGTG TCGTTATTGC AGTGTTGTAC TTTGCAATTC ATGTCAGCAC CGTATTTACT 
GCATTACAGC CGGTTCTGAG CTACGTACTC TATGCACTAG TTGGTTCTGT TGGACTCCTT 
ACTCACTACA TTCTACCACA AGTACGGAAA CAGCTTCCTT GGCACTGCTT CTCCCACCCG 
CTGCTGAAAA CTAAGGAATA TTACCAATTT GAAGTTCGAA ATGCTGCTCA TCTGATGTGG 
TTTGAGAAGG CTCACATCTG GCTGCTTTTT ATCGAGAAGA ACATTATTTA TCCTCTTATA 
GTTCTCAATG AGCTTAGCAT TGGTGCTAAG GATATCGTCA GCCCGAAAAA GCTGGATGCA 
GAAGTTGGTG CATTGTTGAT CACCATTGCC GGAATGAAGC TGCTGAGGTC GTCATTTAGC 
AGTCCCACCT GCCAGTATGC CACTGTCATT TTCACTGTCC TCTTCTTTAC TTTTGACTAC 
AAGGAGTATA CAGAAAAACT GCTGCTAGAT CTGTTCTTTA TGTCAATCCT CTTTAGCAAG 
CTCTGGGAAC TGTTTTTCAA ACTGCGCTTT GTCTACACCT ATATTGCCCC TTGGCAGATC 
ACGTGGGGAT CAGCATTCCA TGCTTTCGCA CAGCCATTTG CTGTACCTCA TTCTGCAATG 
CTGTTTGTAC AGGCAATTGT TTCTGCACTG TTCTCCACTC CCCTGAACCC TTTCTTAGGG 
AGTGCAATCT TTATTACATC ATATGTACGC CCTGTCAAGT TTTGGGAGAG AGACTACAAT 
ACAAAGAGAG TTGACCACTC AAACACAAGA TTAGCCTCTC AGCTTGACAG AAATCCAGGT 
TCAGATGACA ACAACTTGAA TTCCATATTT TATGAGCATC TCACACGCTC CCTTCAGCAT 
AGTCTGTGTG GTGATCTGTT GCTTGGCCGC TGGGGAAATT ACAATACTGG GGATTGCTTT 
ATTCTGGCCT CAGATTATCT AAACGCCTTG GTGCACCTTA TCGAAATAGG CAATGGTCTG 
GTCACCTTCC AGCTAAGAGG ACTAGAGTTC AGAGGTACCT ACTGCCAGCA GCGTGAAGTT 
GAAGCAATCA CTGAGGGTGT AGAGGAAGAT GAAGGCTTTT GCTGTTGCGA ACCTGGACAT 
ATTCCTCATA TGCTGTCTTT CAATGCTGCC TTTGGGCAGC GCTGGCTGGC ATGGGAAGTC 
TTGGTTACAA AGTATGTTCT GGAAGGTTAC AGCATCACAG ACAACAGTGC AGCCTCTATG 
CTTCAAGTGT TTGACCTTCG CAGGATCCTC ACTACCTATT ATGTTAAGGG CATTATATAC 
TATGTGACAG CATCTGCAAA ACTGGAGGAG TGGTTAGCAA ATGAGACAAT GCAGGAAGGT 
CTTCGCCAGT GTTCTGATCA TTGTTATGTT GATGTAGATC CAACTTTCAA CCCAAATATT 
GATGAAGACT ATGACCTTCG CTTAGCAGGC ATTTCACGGG AGAGCTTCTG TGCAAGCTAT 
CTTAACTGGA TTAAGTACTG TTCCTCTAGA AGGGAGAAGC CTCTTGATGC TGAAAAGGAT 
TCACCTTTGG TAACACTTTG TTATGGGCTC TGTATTTTGG GGAGAAGGGC ACTTGGTACA 
GCTTCTCATC ACATGTCCAG CAATTTGGAG TCCTTTCTAT ATGGTTTACA TGCCTTGTTT 
AAAGGAGATT TCCGCATATC ATCTCTCCGT GATGAGTGGA TTTTTGCTGA TATGGAGCTT 
TTAAGAAAAG TAGTAGTTCC TGGCATACGT ATGTCACTAA AACTTCATCA GGACCACTTT 
ACATCTCCAG ATGAGTATGA TGATCCTGCA GTTTTGTATG AAGCCATTAC ATCACATGAG 
CAGAACCTTG TTATTGCGCA TGAGGGAGAT CCAGCGTGGA GGAGTGCTGT TCTTTCTAAC 
TCACCTTCTC TTCTCGCGCT GCGTCATGTG ATGGATGATG GAACCAACGA ATACAAGATC 
ATAATGTTAA ATAAACGTTA TCTTAGTTTT AGAGTCATTA AGGTAAACAA GGAGTGTGTG 
CGGGGCTTAT GGGCTGGTCA GCAGCAAGAA TTGGTCTTTC TTCGTAATCG GAACCCTGAA 
AGAGGCAGCA TCCAAAATGC AAAGCAGGCT CTCAGGAATA TGATCAATTC ATCATGTGAC 
CAACCTATTG GATACCCAAT TTATGTTTCT CCACTGACAA CCTCTTATTC AGACAGCCAT 
GATCAGCTTA AACAGATATT TGGTGGGCCA ATTAGCTTGG CCAATATCCG GAACTTCATT 
GTATCTACAT GGCACAGATT AAGAAAAGGT TGCGGTGCAG GATGCAATAG TGGTGGAAAT 
ATTGAGGACT CTGACACTGG AGGCGGGACC TCCATTAATA GCAACAATCC ACCAAATGCA 
AATGAAACTC AGAGCAGTCT GTCACAAGTT GGTGTCCCAG GTGGAACAGG GCATGTGCCG 
AATGCACGGC TCCAGCCTCC GGGGATGCCT CATCCATCAA CACTAGGAAC CAGTCACAGT 
TCTCACTCCA TGCAGTCAAG CCTTGTCAGA CATTCCCCAG CTCGTGGATC AGTAGCAAGT 
CAATCATCTT ACTGCTACAA CAGCCGTCAC TCATCACTTA GAACATCCAC AACAGGATTT 
GTGCCTTGTA GGCGCTCCTC TACCAGCCAG ATATCTCTTC GCAATCTCCC CTCATCCATT 
CAGTCCCGTC TTTCCATGGT GAACCAAACA GACCCCACAG GACAGTCAGT TTTGGTTGGC 
CTTCCTTCCT CCAGCAGCTC AAGCCAGAGC ATCCCTGCAT GCAAAAGACA CACCTTAGTG 
GGCTTTTTAG GAACAGAAGG AGGCAATAAC CTCACTGAAA CGCAATCCTG TGGCAATTCT 
AGTCCAGCAA CCCATTCAAG AGCCAAAAAG GAAGAGGTTG TTTACAGAGT CCAGATTGTG 
GATCCAAGCC AAGTGCTTGA AGGCGTGAAT ATATCCAAAA GAAAGGAGCT GCAGTGGCCA 
GATGAGCTGG TGCGGCTAAA AGCCGGCAAG AACAGCTGGA AAGACTGGAG CCCTCAGGAA 
GGCATGGAAG GCCATGTAAT TCACCGCTGG GTGCCTTGCA GCAGAGACCC TTGTAATAGG 
TCCCACATTG ACAAAACAAT CCTGCTGATT AAGATTGAGG ACAAATATGT TGCTGTGATT 
GAAACTGGTG TGCTTGAACT CGGAGCTGAA GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pcnx1 ( XM_012969000.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012824454.1 Xenopus tropicalis pecanex homolog (pcnx), transcript variant X5, mRNA.

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CloneID	OXa31182
Clone ID Related Accession (Same CDS sequence)	XM_012969000.2
Accession Version	XM_012969000.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pecanex-like protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012969000.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCC AGACACTGCA AATCCTCCGG CAGGGAGTCT GGGCTTCCAT CACCGGAGGC 
TGGTACTACG ACCCCCACCA GAGTACCTTT GTCAATACCC TACACCTCTA CATCTGGCTC 
TTCCTCCTCT GCTTCCCCTT CACCCTCTAC ATGGCACTAC CCACATCTTT GGTGATTGTT 
GCTGTGTACT GCCCAGTCGT TGCAGCTGTT TTTATTGTTT TGAAGCTTGT TAACTACCGT 
CTGCACAGGG CATTGGATGA AGGAGAAATT GTGGAGAGGA GTGCTAGTGA GTTGACTGAG 
AGGGTGGCAA AGGCAGAACA CAGTGACAGC TCTGCAAGAA GAGAGGACAG CAATGGACCT 
GGTGATCCAG GTGGAGGGAT TGAAATGTCT GAATTTGTTC GAGAAGCAAC TCCTCCAGTT 
GGATGCAGTT CACGTAATTC CTACTCTGGG TTGGACCCAA GCAATCAGGT TGGTGTGTCT 
CGTTATGGAG GTGCATCTGG AAGCAAAGGG GATACAGATG CTGCAAAGAC ATCAGATGAT 
ATCAGTCTGA GTCTTGGCCA GAGTTACAGT TTGTGCAAAG AAGCAAGTGA AGACCAAGAT 
TTGACTGTGG ATCAGAAGCT TTTCCGTCTC GTCTCCAATG ACTCCTTTGT CTCCATGCAG 
CCATCTATAT CCTCGGGTGG AAAAGACTTA ACTAGAGACT TCAGTGACAA AGGATCAATC 
CCAACACACA GTCATCATCA CCATGTGGAT CTGTCTCTGT CTAATGCTTG TGACACAGAA 
TTGGCCTCAC TTGTTCCATT GCATTCACAT TCGTACAGGA AAGATCATCG ACCAAGAGGT 
GTCCCAAGGA CTTCAAGTTC TGCTGTTGCT TTCCCAGACA CTTCTCTCAC TGACTTTTCA 
CTCTATCAAC CAAGGCGCGG GCTGGATCCT GTTAGTGAAC TTGAAGCTTC CAAACCACAT 
TCTGGGTCCA AAGATTCACT AGTACATAAT GCTTGTGCTT CAGGGATTTT TCAATTAGAC 
TGTCTAAAAA GCAAAGGAGC CCAGCAGCTT TCAAGAAGTG GTAGTGTTAA ACACATGAAG 
ACTGACAAAA GCATGGATAG TTTAAGAAGC CTCAGCACGA GAAGCAGTGG ATCCACAGAA 
AGTTATTGTA GTGGAACAGA TCGGGACACA AATAGCACTG TTAGCAGCTA CAAAAGTGAA 
CAGACCAGTT CAACTCATGT TGAAAGTATT TTATCTGAGC ATGAGGATGT TACTGCTGCA 
AAGAGAGTCC ATTGTAGTGA CCTGGAGGAA GAACATAGCT CTGCTACTGA TAAAAGGACT 
AGTAGTATAA GAACTGCTTT AGAAAGAAAC ACTGCTCAGG AAGCCAAGGA TGCCAACACA 
TCTGATGATG TACAAAGTCC AAGGGGTCTT AGCTCATCAG CATCAGAGGA AGCTAATAAG 
AATCCACACG CAAATGAGTT AACTGTACAA ACAGACTGGC TTCCAGGTAA ACTGTCAGTG 
AACATTGATG AACCTGGGGA GAAACCAGTC ATTTTAGTTG ACTTGAGATC TTTGAAAGAA 
ACGGGTATTA AGCAGAGAGA CAATGACGTA CGACCCAAAT CATCTAGTTT AATACATCGA 
ACATCCTCTG CTCATAAATC ATGCAGGAGA CGGACAGGTA AAAAAAGAGC CAGCAGTTTT 
GATGCAAGTA GACACAAAGA ATATGTTTCC TTTCAAGGTA TAGCTGATAC CAAACCTCAC 
AGTACTGGCT TTTGTCATGA TGAAGATTCT AGCGACCAAA GCGACTTGAG CAGGACCTCA 
AGCGTTCAGT CGGCCCACCG TTTCAGCAGC GATAGCTCTT CCAGCACTAC TTCCCATTCA 
TGCCAGTCTC CTGAAGCAAG ATACAGTGCC CTAAGGACAA AATATCGAAT AAAAGAAAGA 
GGCACAGATT CACATCACAC ACACCTAAAT TGTGAAGGAA CTAGTAAAAA GCGGTCCAAC 
CGCAGGACTT CTAGTGCAAG TGGTACAAAA AGCCGAGCTA GAGTATTAAG TCTGGACAGT 
GGCACTGTAG TTAGTTTGAG TGATGCCAAC AGACTGGCCG CTCCTGAAAA TATAAAAACC 
TTAACCACTT CAAAATCAGA TCTAGAAGCC AAAGAAGGCG AAGTGCTAGA TCAGATGTCT 
CTGTTGGGGC GAGCTTCACA GTTAGAATCT GTAACACGGT CAAGAAATAG TTTGCCCAGC 
CAGGTTACTT TCCAAGATGT TGGAGAGCGA GACGCATCTG TTGGAGGTAA ATTTACCCCA 
TCTCTATATG AGACTGGAGG TTGTGACATG TCACTTGTAA ACTTTGAGCC AGCTGCAAGA 
AGGACGTCTA ATAATCTTTG TGACACAGAT TCCCATGTAT CAAGTTCTAC CTCAGTTCGG 
TTCTACCCAC ATGATCTACT CTCTCTTCCC CAGATCCGAT TAAACAGGCT GCTAACCATA 
GATACAGATC TGTTAGAGCA GCAGGATATT GATCTAAGCC CTGACCTGCA GGACCAGATG 
CAACCACAGG AACAGGCTGC CCAAAAAGTA AAACACTACT ACCGGTTTTG GATTCTCCCA 
AAGCTGTGGG TCGGCATCAA CTTTGACAGG CTTACACTCT TAGCCTTGTT CGATAGGAAC 
CGCGAGGTCC TAGAAAATGT GTTGTCTGTC ATTCTCGCCA TTCTAGTTGC CTTTTTGGGC 
TCTGTTCTTC TCATAGAAGG CTTCTTTAAA GATATCTGGG TCTTCCAGTT TTGTCTTGTT 
ATAGCCAGCT GTCAATACTC TCTCCTTAAG AGTGTTCAAC CAGATTCTTC TTCCCCAAGA 
CATGGCCATA ATCGTATCAT TGCCTATAGT AGGCCCGTTT ACTTCTGTGC CTGTTGCGGC 
CTTATTTGGT TATTGGATTA TGGAAGCAAA AACATGTCTA CCACCAAATA CAAACTATAT 
GGAATGGCCT TCACCAATCC TCTGCTTCTA GTGTCAGCCA GGGACTTAGT CATTGTCTTC 
ACACTCTGTT TCCCAGTCGT GTTCTTTATT GGTCTCCTGC CTCAGGTGAA CACATTCGTC 
ATGTACCTGT GTGAGCAAGT AGATATACAT GTGTTTGGTG GAAATGCTAC CACCAGCTTG 
CTTGCAGCAA TTTACAGCTT TATCCGTAGC ATTGTGGCCA TAGCTTTACT CTACGGGCTG 
TGTTACGGTG CTCTTAAGGA TCCATGGGAT GCACAACACA TTCCTGTATT ATTCTCTATT 
TTCTGTGGTG TTCTTGTGGC AGTTTCATAC CATCTCAGCC GACAGAGCAG CGATCCCTCT 
GTCCTCATCT CACTGGTGCA ATCAAAGATT TTTCCAAACA GCGAGGATAC AAATCCTGAA 
GACCCACTAT CAGAAGTGAA AGACCCTCTT CCAGAAAAAC TGAGAAGCTC TGTTAATGAA 
CGACTTCAGT CAGACGTAGT AGTCTGTGTC GTTATTGCAG TGTTGTACTT TGCAATTCAT 
GTCAGCACCG TATTTACTGC ATTACAGCCG GTTCTGAGCT ACGTACTCTA TGCACTAGTT 
GGTTCTGTTG GACTCCTTAC TCACTACATT CTACCACAAG TACGGAAACA GCTTCCTTGG 
CACTGCTTCT CCCACCCGCT GCTGAAAACT AAGGAATATT ACCAATTTGA AGTTCGAAAT 
GCTGCTCATC TGATGTGGTT TGAGAAGGCT CACATCTGGC TGCTTTTTAT CGAGAAGAAC 
ATTATTTATC CTCTTATAGT TCTCAATGAG CTTAGCATTG GTGCTAAGGA TATCGTCAGC 
CCGAAAAAGC TGGATGCAGA AGTTGGTGCA TTGTTGATCA CCATTGCCGG AATGAAGCTG 
CTGAGGTCGT CATTTAGCAG TCCCACCTGC CAGTATGCCA CTGTCATTTT CACTGTCCTC 
TTCTTTACTT TTGACTACAA GGAGTATACA GAAAAACTGC TGCTAGATCT GTTCTTTATG 
TCAATCCTCT TTAGCAAGCT CTGGGAACTG TTTTTCAAAC TGCGCTTTGT CTACACCTAT 
ATTGCCCCTT GGCAGATCAC GTGGGGATCA GCATTCCATG CTTTCGCACA GCCATTTGCT 
GTACCTCATT CTGCAATGCT GTTTGTACAG GCAATTGTTT CTGCACTGTT CTCCACTCCC 
CTGAACCCTT TCTTAGGGAG TGCAATCTTT ATTACATCAT ATGTACGCCC TGTCAAGTTT 
TGGGAGAGAG ACTACAATAC AAAGAGAGTT GACCACTCAA ACACAAGATT AGCCTCTCAG 
CTTGACAGAA ATCCAGGTTC AGATGACAAC AACTTGAATT CCATATTTTA TGAGCATCTC 
ACACGCTCCC TTCAGCATAG TCTGTGTGGT GATCTGTTGC TTGGCCGCTG GGGAAATTAC 
AATACTGGGG ATTGCTTTAT TCTGGCCTCA GATTATCTAA ACGCCTTGGT GCACCTTATC 
GAAATAGGCA ATGGTCTGGT CACCTTCCAG CTAAGAGGAC TAGAGTTCAG AGGTACCTAC 
TGCCAGCAGC GTGAAGTTGA AGCAATCACT GAGGGTGTAG AGGAAGATGA AGGCTTTTGC 
TGTTGCGAAC CTGGACATAT TCCTCATATG CTGTCTTTCA ATGCTGCCTT TGGGCAGCGC 
TGGCTGGCAT GGGAAGTCTT GGTTACAAAG TATGTTCTGG AAGGTTACAG CATCACAGAC 
AACAGTGCAG CCTCTATGCT TCAAGTGTTT GACCTTCGCA GGATCCTCAC TACCTATTAT 
GTTAAGGGCA TTATATACTA TGTGACAGCA TCTGCAAAAC TGGAGGAGTG GTTAGCAAAT 
GAGACAATGC AGGAAGGTCT TCGCCAGTGT TCTGATCATT GTTATGTTGA TGTAGATCCA 
ACTTTCAACC CAAATATTGA TGAAGACTAT GACCTTCGCT TAGCAGGCAT TTCACGGGAG 
AGCTTCTGTG CAAGCTATCT TAACTGGATT AAGTACTGTT CCTCTAGAAG GGAGAAGCCT 
CTTGATGCTG AAAAGGATTC ACCTTTGGTA ACACTTTGTT ATGGGCTCTG TATTTTGGGG 
AGAAGGGCAC TTGGTACAGC TTCTCATCAC ATGTCCAGCA ATTTGGAGTC CTTTCTATAT 
GGTTTACATG CCTTGTTTAA AGGAGATTTC CGCATATCAT CTCTCCGTGA TGAGTGGATT 
TTTGCTGATA TGGAGCTTTT AAGAAAAGTA GTAGTTCCTG GCATACGTAT GTCACTAAAA 
CTTCATCAGG ACCACTTTAC ATCTCCAGAT GAGTATGATG ATCCTGCAGT TTTGTATGAA 
GCCATTACAT CACATGAGCA GAACCTTGTT ATTGCGCATG AGGGAGATCC AGCGTGGAGG 
AGTGCTGTTC TTTCTAACTC ACCTTCTCTT CTCGCGCTGC GTCATGTGAT GGATGATGGA 
ACCAACGAAT ACAAGATCAT AATGTTAAAT AAACGTTATC TTAGTTTTAG AGTCATTAAG 
GTAAACAAGG AGTGTGTGCG GGGCTTATGG GCTGGTCAGC AGCAAGAATT GGTCTTTCTT 
CGTAATCGGA ACCCTGAAAG AGGCAGCATC CAAAATGCAA AGCAGGCTCT CAGGAATATG 
ATCAATTCAT CATGTGACCA ACCTATTGGA TACCCAATTT ATGTTTCTCC ACTGACAACC 
TCTTATTCAG ACAGCCATGA TCAGCTTAAA CAGATATTTG GTGGGCCAAT TAGCTTGGCC 
AATATCCGGA ACTTCATTGT ATCTACATGG CACAGATTAA GAAAAGGTTG CGGTGCAGGA 
TGCAATAGTG GTGGAAATAT TGAGGACTCT GACACTGGAG GCGGGACCTC CATTAATAGC 
AACAATCCAC CAAATGCAAA TGAAACTCAG AGCAGTCTGT CACAAGTTGG TGTCCCAGGT 
GGAACAGGGC ATGTGCCGAA TGCACGGCTC CAGCCTCCGG GGATGCCTCA TCCATCAACA 
CTAGGAACCA GTCACAGTTC TCACTCCATG CAGTCAAGCC TTGTCAGACA TTCCCCAGCT 
CGTGGATCAG TAGCAAGTCA ATCATCTTAC TGCTACAACA GCCGTCACTC ATCACTTAGA 
ACATCCACAA CAGGATTTGT GCCTTGTAGG CGCTCCTCTA CCAGCCAGAT ATCTCTTCGC 
AATCTCCCCT CATCCATTCA GTCCCGTCTT TCCATGGTGA ACCAAACAGA CCCCACAGGA 
CAGTCAGTTT TGGTTGGCCT TCCTTCCTCC AGCAGCTCAA GCCAGAGCAT CCCTGCATGC 
AAAAGACACA CCTTAGTGGG CTTTTTAGGA ACAGAAGGAG GCAATAACCT CACTGAAACG 
CAATCCTGTG GCAATTCTAG TCCAGCAACC CATTCAAGAG CCAAAAAGGA AGAGGTTGTT 
TACAGAGTCC AGATTGTGGA TCCAAGCCAA GTGCTTGAAG GCGTGAATAT ATCCAAAAGA 
AAGGAGCTGC AGTGGCCAGA TGAGCTGGTG CGGCTAAAAG CCGGCAAGAA CAGCTGGAAA 
GACTGGAGCC CTCAGGAAGG CATGGAAGGC CATGTAATTC ACCGCTGGGT GCCTTGCAGC 
AGAGACCCTT GTAATAGGTC CCACATTGAC AAAACAATCC TGCTGATTAA GATTGAGGAC 
AAATATGTTG CTGTGATTGA AACTGGTGTG CTTGAACTCG GAGCTGAAGT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012824454.1
CDS	272..6925
Translation	MGSQTLQILRQGVWASITGGWYYDPHQSTFVNTLHLYIWLFLLCFPFTLYMALPTSLVIVAVYCPVVAAVFIVLKLVNYRLHRALDEGEIVERSASELTERVAKAEHSDSSARREDSNGPGDPGGGIEMSEFVREATPPVGCSSRNSYSGLDPSNQVGVSRYGGASGSKGDTDAAKTSDDISLSLGQSYSLCKEASEDQDLTVDQKLFRLVSNDSFVSMQPSISSGGKDLTRDFSDKGSIPTHSHHHHVDLSLSNACDTELASLVPLHSHSYRKDHRPRGVPRTSSSAVAFPDTSLTDFSLYQPRRGLDPVSELEASKPHSGSKDSLVHNACASGIFQLDCLKSKGAQQLSRSGSVKHMKTDKSMDSLRSLSTRSSGSTESYCSGTDRDTNSTVSSYKSEQTSSTHVESILSEHEDVTAAKRVHCSDLEEEHSSATDKRTSSIRTALERNTAQEAKDANTSDDVQSPRGLSSSASEEANKNPHANELTVQTDWLPGKLSVNIDEPGEKPVILVDLRSLKETGIKQRDNDVRPKSSSLIHRTSSAHKSCRRRTGKKRASSFDASRHKEYVSFQGIADTKPHSTGFCHDEDSSDQSDLSRTSSVQSAHRFSSDSSSSTTSHSCQSPEARYSALRTKYRIKERGTDSHHTHLNCEGTSKKRSNRRTSSASGTKSRARVLSLDSGTVVSLSDANRLAAPENIKTLTTSKSDLEAKEGEVLDQMSLLGRASQLESVTRSRNSLPSQVTFQDVGERDASVGGKFTPSLYETGGCDMSLVNFEPAARRTSNNLCDTDSHVSSSTSVRFYPHDLLSLPQIRLNRLLTIDTDLLEQQDIDLSPDLQDQMQPQEQAAQKVKHYYRFWILPKLWVGINFDRLTLLALFDRNREVLENVLSVILAILVAFLGSVLLIEGFFKDIWVFQFCLVIASCQYSLLKSVQPDSSSPRHGHNRIIAYSRPVYFCACCGLIWLLDYGSKNMSTTKYKLYGMAFTNPLLLVSARDLVIVFTLCFPVVFFIGLLPQVNTFVMYLCEQVDIHVFGGNATTSLLAAIYSFIRSIVAIALLYGLCYGALKDPWDAQHIPVLFSIFCGVLVAVSYHLSRQSSDPSVLISLVQSKIFPNSEDTNPEDPLSEVKDPLPEKLRSSVNERLQSDVVVCVVIAVLYFAIHVSTVFTALQPVLSYVLYALVGSVGLLTHYILPQVRKQLPWHCFSHPLLKTKEYYQFEVRNAAHLMWFEKAHIWLLFIEKNIIYPLIVLNELSIGAKDIVSPKKLDAEVGALLITIAGMKLLRSSFSSPTCQYATVIFTVLFFTFDYKEYTEKLLLDLFFMSILFSKLWELFFKLRFVYTYIAPWQITWGSAFHAFAQPFAVPHSAMLFVQAIVSALFSTPLNPFLGSAIFITSYVRPVKFWERDYNTKRVDHSNTRLASQLDRNPGSDDNNLNSIFYEHLTRSLQHSLCGDLLLGRWGNYNTGDCFILASDYLNALVHLIEIGNGLVTFQLRGLEFRGTYCQQREVEAITEGVEEDEGFCCCEPGHIPHMLSFNAAFGQRWLAWEVLVTKYVLEGYSITDNSAASMLQVFDLRRILTTYYVKGIIYYVTASAKLEEWLANETMQEGLRQCSDHCYVDVDPTFNPNIDEDYDLRLAGISRESFCASYLNWIKYCSSRREKPLDAEKDSPLVTLCYGLCILGRRALGTASHHMSSNLESFLYGLHALFKGDFRISSLRDEWIFADMELLRKVVVPGIRMSLKLHQDHFTSPDEYDDPAVLYEAITSHEQNLVIAHEGDPAWRSAVLSNSPSLLALRHVMDDGTNEYKIIMLNKRYLSFRVIKVNKECVRGLWAGQQQELVFLRNRNPERGSIQNAKQALRNMINSSCDQPIGYPIYVSPLTTSYSDSHDQLKQIFGGPISLANIRNFIVSTWHRLRKGCGAGCNSGGNIEDSDTGGGTSINSNNPPNANETQSSLSQVGVPGGTGHVPNARLQPPGMPHPSTLGTSHSSHSMQSSLVRHSPARGSVASQSSYCYNSRHSSLRTSTTGFVPCRRSSTSQISLRNLPSSIQSRLSMVNQTDPTGQSVLVGLPSSSSSSQSIPACKRHTLVGFLGTEGGNNLTETQSCGNSSPATHSRAKKEEVVYRVQIVDPSQVLEGVNISKRKELQWPDELVRLKAGKNSWKDWSPQEGMEGHVIHRWVPCSRDPCNRSHIDKTILLIKIEDKYVAVIETGVLELGAEV

Target ORF information:

RefSeq Version	XM_012969000.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pecanex homolog (pcnx), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012969000.2

ORF Insert Sequence:

ATGGGTTCCC AGACACTGCA AATCCTCCGG CAGGGAGTCT GGGCTTCCAT CACCGGAGGC 
TGGTACTACG ACCCCCACCA GAGTACCTTT GTCAATACCC TACACCTCTA CATCTGGCTC 
TTCCTCCTCT GCTTCCCCTT CACCCTCTAC ATGGCACTAC CCACATCTTT GGTGATTGTT 
GCTGTGTACT GCCCAGTCGT TGCAGCTGTT TTTATTGTTT TGAAGCTTGT TAACTACCGT 
CTGCACAGGG CATTGGATGA AGGAGAAATT GTGGAGAGGA GTGCTAGTGA GTTGACTGAG 
AGGGTGGCAA AGGCAGAACA CAGTGACAGC TCTGCAAGAA GAGAGGACAG CAATGGACCT 
GGTGATCCAG GTGGAGGGAT TGAAATGTCT GAATTTGTTC GAGAAGCAAC TCCTCCAGTT 
GGATGCAGTT CACGTAATTC CTACTCTGGG TTGGACCCAA GCAATCAGGT TGGTGTGTCT 
CGTTATGGAG GTGCATCTGG AAGCAAAGGG GATACAGATG CTGCAAAGAC ATCAGATGAT 
ATCAGTCTGA GTCTTGGCCA GAGTTACAGT TTGTGCAAAG AAGCAAGTGA AGACCAAGAT 
TTGACTGTGG ATCAGAAGCT TTTCCGTCTC GTCTCCAATG ACTCCTTTGT CTCCATGCAG 
CCATCTATAT CCTCGGGTGG AAAAGACTTA ACTAGAGACT TCAGTGACAA AGGATCAATC 
CCAACACACA GTCATCATCA CCATGTGGAT CTGTCTCTGT CTAATGCTTG TGACACAGAA 
TTGGCCTCAC TTGTTCCATT GCATTCACAT TCGTACAGGA AAGATCATCG ACCAAGAGGT 
GTCCCAAGGA CTTCAAGTTC TGCTGTTGCT TTCCCAGACA CTTCTCTCAC TGACTTTTCA 
CTCTATCAAC CAAGGCGCGG GCTGGATCCT GTTAGTGAAC TTGAAGCTTC CAAACCACAT 
TCTGGGTCCA AAGATTCACT AGTACATAAT GCTTGTGCTT CAGGGATTTT TCAATTAGAC 
TGTCTAAAAA GCAAAGGAGC CCAGCAGCTT TCAAGAAGTG GTAGTGTTAA ACACATGAAG 
ACTGACAAAA GCATGGATAG TTTAAGAAGC CTCAGCACGA GAAGCAGTGG ATCCACAGAA 
AGTTATTGTA GTGGAACAGA TCGGGACACA AATAGCACTG TTAGCAGCTA CAAAAGTGAA 
CAGACCAGTT CAACTCATGT TGAAAGTATT TTATCTGAGC ATGAGGATGT TACTGCTGCA 
AAGAGAGTCC ATTGTAGTGA CCTGGAGGAA GAACATAGCT CTGCTACTGA TAAAAGGACT 
AGTAGTATAA GAACTGCTTT AGAAAGAAAC ACTGCTCAGG AAGCCAAGGA TGCCAACACA 
TCTGATGATG TACAAAGTCC AAGGGGTCTT AGCTCATCAG CATCAGAGGA AGCTAATAAG 
AATCCACACG CAAATGAGTT AACTGTACAA ACAGACTGGC TTCCAGGTAA ACTGTCAGTG 
AACATTGATG AACCTGGGGA GAAACCAGTC ATTTTAGTTG ACTTGAGATC TTTGAAAGAA 
ACGGGTATTA AGCAGAGAGA CAATGACGTA CGACCCAAAT CATCTAGTTT AATACATCGA 
ACATCCTCTG CTCATAAATC ATGCAGGAGA CGGACAGGTA AAAAAAGAGC CAGCAGTTTT 
GATGCAAGTA GACACAAAGA ATATGTTTCC TTTCAAGGTA TAGCTGATAC CAAACCTCAC 
AGTACTGGCT TTTGTCATGA TGAAGATTCT AGCGACCAAA GCGACTTGAG CAGGACCTCA 
AGCGTTCAGT CGGCCCACCG TTTCAGCAGC GATAGCTCTT CCAGCACTAC TTCCCATTCA 
TGCCAGTCTC CTGAAGCAAG ATACAGTGCC CTAAGGACAA AATATCGAAT AAAAGAAAGA 
GGCACAGATT CACATCACAC ACACCTAAAT TGTGAAGGAA CTAGTAAAAA GCGGTCCAAC 
CGCAGGACTT CTAGTGCAAG TGGTACAAAA AGCCGAGCTA GAGTATTAAG TCTGGACAGT 
GGCACTGTAG TTAGTTTGAG TGATGCCAAC AGACTGGCCG CTCCTGAAAA TATAAAAACC 
TTAACCACTT CAAAATCAGA TCTAGAAGCC AAAGAAGGCG AAGTGCTAGA TCAGATGTCT 
CTGTTGGGGC GAGCTTCACA GTTAGAATCT GTAACACGGT CAAGAAATAG TTTGCCCAGC 
CAGGTTACTT TCCAAGATGT TGGAGAGCGA GACGCATCTG TTGGAGGTAA ATTTACCCCA 
TCTCTATATG AGACTGGAGG TTGTGACATG TCACTTGTAA ACTTTGAGCC AGCTGCAAGA 
AGGACGTCTA ATAATCTTTG TGACACAGAT TCCCATGTAT CAAGTTCTAC CTCAGTTCGG 
TTCTACCCAC ATGATCTACT CTCTCTTCCC CAGATCCGAT TAAACAGGCT GCTAACCATA 
GATACAGATC TGTTAGAGCA GCAGGATATT GATCTAAGCC CTGACCTGCA GGACCAGATG 
CAACCACAGG AACAGGCTGC CCAAAAAGTA AAACACTACT ACCGGTTTTG GATTCTCCCA 
AAGCTGTGGG TCGGCATCAA CTTTGACAGG CTTACACTCT TAGCCTTGTT CGATAGGAAC 
CGCGAGGTCC TAGAAAATGT GTTGTCTGTC ATTCTCGCCA TTCTAGTTGC CTTTTTGGGC 
TCTGTTCTTC TCATAGAAGG CTTCTTTAAA GATATCTGGG TCTTCCAGTT TTGTCTTGTT 
ATAGCCAGCT GTCAATACTC TCTCCTTAAG AGTGTTCAAC CAGATTCTTC TTCCCCAAGA 
CATGGCCATA ATCGTATCAT TGCCTATAGT AGGCCCGTTT ACTTCTGTGC CTGTTGCGGC 
CTTATTTGGT TATTGGATTA TGGAAGCAAA AACATGTCTA CCACCAAATA CAAACTATAT 
GGAATGGCCT TCACCAATCC TCTGCTTCTA GTGTCAGCCA GGGACTTAGT CATTGTCTTC 
ACACTCTGTT TCCCAGTCGT GTTCTTTATT GGTCTCCTGC CTCAGGTGAA CACATTCGTC 
ATGTACCTGT GTGAGCAAGT AGATATACAT GTGTTTGGTG GAAATGCTAC CACCAGCTTG 
CTTGCAGCAA TTTACAGCTT TATCCGTAGC ATTGTGGCCA TAGCTTTACT CTACGGGCTG 
TGTTACGGTG CTCTTAAGGA TCCATGGGAT GCACAACACA TTCCTGTATT ATTCTCTATT 
TTCTGTGGTG TTCTTGTGGC AGTTTCATAC CATCTCAGCC GACAGAGCAG CGATCCCTCT 
GTCCTCATCT CACTGGTGCA ATCAAAGATT TTTCCAAACA GCGAGGATAC AAATCCTGAA 
GACCCACTAT CAGAAGTGAA AGACCCTCTT CCAGAAAAAC TGAGAAGCTC TGTTAATGAA 
CGACTTCAGT CAGACGTAGT AGTCTGTGTC GTTATTGCAG TGTTGTACTT TGCAATTCAT 
GTCAGCACCG TATTTACTGC ATTACAGCCG GTTCTGAGCT ACGTACTCTA TGCACTAGTT 
GGTTCTGTTG GACTCCTTAC TCACTACATT CTACCACAAG TACGGAAACA GCTTCCTTGG 
CACTGCTTCT CCCACCCGCT GCTGAAAACT AAGGAATATT ACCAATTTGA AGTTCGAAAT 
GCTGCTCATC TGATGTGGTT TGAGAAGGCT CACATCTGGC TGCTTTTTAT CGAGAAGAAC 
ATTATTTATC CTCTTATAGT TCTCAATGAG CTTAGCATTG GTGCTAAGGA TATCGTCAGC 
CCGAAAAAGC TGGATGCAGA AGTTGGTGCA TTGTTGATCA CCATTGCCGG AATGAAGCTG 
CTGAGGTCGT CATTTAGCAG TCCCACCTGC CAGTATGCCA CTGTCATTTT CACTGTCCTC 
TTCTTTACTT TTGACTACAA GGAGTATACA GAAAAACTGC TGCTAGATCT GTTCTTTATG 
TCAATCCTCT TTAGCAAGCT CTGGGAACTG TTTTTCAAAC TGCGCTTTGT CTACACCTAT 
ATTGCCCCTT GGCAGATCAC GTGGGGATCA GCATTCCATG CTTTCGCACA GCCATTTGCT 
GTACCTCATT CTGCAATGCT GTTTGTACAG GCAATTGTTT CTGCACTGTT CTCCACTCCC 
CTGAACCCTT TCTTAGGGAG TGCAATCTTT ATTACATCAT ATGTACGCCC TGTCAAGTTT 
TGGGAGAGAG ACTACAATAC AAAGAGAGTT GACCACTCAA ACACAAGATT AGCCTCTCAG 
CTTGACAGAA ATCCAGGTTC AGATGACAAC AACTTGAATT CCATATTTTA TGAGCATCTC 
ACACGCTCCC TTCAGCATAG TCTGTGTGGT GATCTGTTGC TTGGCCGCTG GGGAAATTAC 
AATACTGGGG ATTGCTTTAT TCTGGCCTCA GATTATCTAA ACGCCTTGGT GCACCTTATC 
GAAATAGGCA ATGGTCTGGT CACCTTCCAG CTAAGAGGAC TAGAGTTCAG AGGTACCTAC 
TGCCAGCAGC GTGAAGTTGA AGCAATCACT GAGGGTGTAG AGGAAGATGA AGGCTTTTGC 
TGTTGCGAAC CTGGACATAT TCCTCATATG CTGTCTTTCA ATGCTGCCTT TGGGCAGCGC 
TGGCTGGCAT GGGAAGTCTT GGTTACAAAG TATGTTCTGG AAGGTTACAG CATCACAGAC 
AACAGTGCAG CCTCTATGCT TCAAGTGTTT GACCTTCGCA GGATCCTCAC TACCTATTAT 
GTTAAGGGCA TTATATACTA TGTGACAGCA TCTGCAAAAC TGGAGGAGTG GTTAGCAAAT 
GAGACAATGC AGGAAGGTCT TCGCCAGTGT TCTGATCATT GTTATGTTGA TGTAGATCCA 
ACTTTCAACC CAAATATTGA TGAAGACTAT GACCTTCGCT TAGCAGGCAT TTCACGGGAG 
AGCTTCTGTG CAAGCTATCT TAACTGGATT AAGTACTGTT CCTCTAGAAG GGAGAAGCCT 
CTTGATGCTG AAAAGGATTC ACCTTTGGTA ACACTTTGTT ATGGGCTCTG TATTTTGGGG 
AGAAGGGCAC TTGGTACAGC TTCTCATCAC ATGTCCAGCA ATTTGGAGTC CTTTCTATAT 
GGTTTACATG CCTTGTTTAA AGGAGATTTC CGCATATCAT CTCTCCGTGA TGAGTGGATT 
TTTGCTGATA TGGAGCTTTT AAGAAAAGTA GTAGTTCCTG GCATACGTAT GTCACTAAAA 
CTTCATCAGG ACCACTTTAC ATCTCCAGAT GAGTATGATG ATCCTGCAGT TTTGTATGAA 
GCCATTACAT CACATGAGCA GAACCTTGTT ATTGCGCATG AGGGAGATCC AGCGTGGAGG 
AGTGCTGTTC TTTCTAACTC ACCTTCTCTT CTCGCGCTGC GTCATGTGAT GGATGATGGA 
ACCAACGAAT ACAAGATCAT AATGTTAAAT AAACGTTATC TTAGTTTTAG AGTCATTAAG 
GTAAACAAGG AGTGTGTGCG GGGCTTATGG GCTGGTCAGC AGCAAGAATT GGTCTTTCTT 
CGTAATCGGA ACCCTGAAAG AGGCAGCATC CAAAATGCAA AGCAGGCTCT CAGGAATATG 
ATCAATTCAT CATGTGACCA ACCTATTGGA TACCCAATTT ATGTTTCTCC ACTGACAACC 
TCTTATTCAG ACAGCCATGA TCAGCTTAAA CAGATATTTG GTGGGCCAAT TAGCTTGGCC 
AATATCCGGA ACTTCATTGT ATCTACATGG CACAGATTAA GAAAAGGTTG CGGTGCAGGA 
TGCAATAGTG GTGGAAATAT TGAGGACTCT GACACTGGAG GCGGGACCTC CATTAATAGC 
AACAATCCAC CAAATGCAAA TGAAACTCAG AGCAGTCTGT CACAAGTTGG TGTCCCAGGT 
GGAACAGGGC ATGTGCCGAA TGCACGGCTC CAGCCTCCGG GGATGCCTCA TCCATCAACA 
CTAGGAACCA GTCACAGTTC TCACTCCATG CAGTCAAGCC TTGTCAGACA TTCCCCAGCT 
CGTGGATCAG TAGCAAGTCA ATCATCTTAC TGCTACAACA GCCGTCACTC ATCACTTAGA 
ACATCCACAA CAGGATTTGT GCCTTGTAGG CGCTCCTCTA CCAGCCAGAT ATCTCTTCGC 
AATCTCCCCT CATCCATTCA GTCCCGTCTT TCCATGGTGA ACCAAACAGA CCCCACAGGA 
CAGTCAGTTT TGGTTGGCCT TCCTTCCTCC AGCAGCTCAA GCCAGAGCAT CCCTGCATGC 
AAAAGACACA CCTTAGTGGG CTTTTTAGGA ACAGAAGGAG GCAATAACCT CACTGAAACG 
CAATCCTGTG GCAATTCTAG TCCAGCAACC CATTCAAGAG CCAAAAAGGA AGAGGTTGTT 
TACAGAGTCC AGATTGTGGA TCCAAGCCAA GTGCTTGAAG GCGTGAATAT ATCCAAAAGA 
AAGGAGCTGC AGTGGCCAGA TGAGCTGGTG CGGCTAAAAG CCGGCAAGAA CAGCTGGAAA 
GACTGGAGCC CTCAGGAAGG CATGGAAGGC CATGTAATTC ACCGCTGGGT GCCTTGCAGC 
AGAGACCCTT GTAATAGGTC CCACATTGAC AAAACAATCC TGCTGATTAA GATTGAGGAC 
AAATATGTTG CTGTGATTGA AACTGGTGTG CTTGAACTCG GAGCTGAAGT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pcnx1 ( XM_012969001.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012824455.1 Xenopus tropicalis pecanex homolog (pcnx), transcript variant X6, mRNA.

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CloneID	OXa31183
Clone ID Related Accession (Same CDS sequence)	XM_012969001.2
Accession Version	XM_012969001.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6639bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pecanex-like protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012969001.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCC AGACACTGCA AATCCTCCGG CAGGGAGTCT GGGCTTCCAT CACCGGAGGC 
TGGTACTACG ACCCCCACCA GAGTACCTTT GTCAATACCC TACACCTCTA CATCTGGCTC 
TTCCTCCTCT GCTTCCCCTT CACCCTCTAC ATGGCACTAC CCACATCTTT GGTGATTGTT 
GCTGTGTACT GCCCAGTCGT TGCAGCTGTT TTTATTGTTT TGAAGCTTGT TAACTACCGT 
CTGCACAGGG CATTGGATGA AGGAGAAATT GTGGAGAGGA GTGCTAGTGA GTTGACTGAG 
AGGGTGGCAA AGGCAGAACA CAGTGACAGC TCTGCAAGAA GAGAGGACAG CAATGGACCT 
GGTGATCCAG GTGGAGGGAT TGAAATGTCT GAATTTGTTC GAGAAGCAAC TCCTCCAGTT 
GGATGCAGTT CACGTAATTC CTACTCTGGG TTGGACCCAA GCAATCAGGT TGGTGTGTCT 
CGTTATGGAG GTGCATCTGG AAGCAAAGGG GATACAGATG CTGCAAAGAC ATCAGATGAT 
ATCAGTCTGA GTCTTGGCCA GAGTTACAGT TTGTGCAAAG AAGCAAGTGA AGACCAAGAT 
TTGACTGTGG ATCAGAAGCT TTTCCGTCTC GTCTCCAATG ACTCCTTTGT CTCCATGCAG 
CCATCTATAT CCTCGGGTGG AAAAGACTTA ACTAGAGACT TCAGTGACAA AGGATCAATC 
CCAACACACA GTCATCATCA CCATGTGGAT CTGTCTCTGT CTAATGCTTG TGACACAGAA 
TTGGCCTCAC TTGTTCCATT GCATTCACAT TCGTACAGGA AAGATCATCG ACCAAGAGGT 
GTCCCAAGGA CTTCAAGTTC TGCTGTTGCT TTCCCAGACA CTTCTCTCAC TGACTTTTCA 
CTCTATCAAC CAAGGCGCGG GCTGGATCCT GTTAGTGAAC TTGAAGCTTC CAAACCACAT 
TCTGGGTCCA AAGATTCACT AGTACATAAT GCTTGTGCTT CAGGGATTTT TCAATTAGAC 
TGTCTAAAAA GCAAAGGAGC CCAGCAGCTT TCAAGAAGTG GTAGTGTTAA ACACATGAAG 
ACTGACAAAA GCATGGATAG TTTAAGAAGC CTCAGCACGA GAAGCAGTGG ATCCACAGAA 
AGTTATTGTA GTGGAACAGA TCGGGACACA AATAGCACTG TTAGCAGCTA CAAAAGTGAA 
CAGACCAGTT CAACTCATGT TGAAAGTATT TTATCTGAGC ATGAGGATGT TACTGCTGCA 
AAGAGAGTCC ATTGTAGTGA CCTGGAGGAA GAACATAGCT CTGCTACTGA TAAAAGGACT 
AGTAGTATAA GAACTGCTTT AGAAAGAAAC ACTGCTCAGG AAGCCAAGGA TGCCAACACA 
TCTGATGATG TACAAAGTCC AAGGGGTCTT AGCTCATCAG CATCAGAGGA AGCTAATAAG 
AATCCACACG CAAATGAGTT AACTGTACAA ACAGACTGGC TTCCAGGTAA ACTGTCAGTG 
AACATTGATG AACCTGGGGA GAAACCAGTC ATTTTAGTTG ACTTGAGATC TTTGAAAGAA 
ACGGGTATTA AGCAGAGAGA CAATGACGTA CGACCCAAAT CATCTAGTTT AATACATCGA 
ACATCCTCTG CTCATAAATC ATGCAGGAGA CGGACAGGTA AAAAAAGAGC CAGCAGTTTT 
GATGCAAGTA GACACAAAGA ATATGTTTCC TTTCAAGGTA TAGCTGATAC CAAACCTCAC 
AGTACTGGCT TTTGTCATGA TGAAGATTCT AGCGACCAAA GCGACTTGAG CAGGACCTCA 
AGCGTTCAGT CGGCCCACCG TTTCAGCAGC GATAGCTCTT CCAGCACTAC TTCCCATTCA 
TGCCAGTCTC CTGAAGCAAG ATACAGTGCC CTAAGGACAA AATATCGAAT AAAAGAAAGA 
GGCACAGATT CACATCACAC ACACCTAAAT TGTGAAGGAA CTAGTAAAAA GCGGTCCAAC 
CGCAGGACTT CTAGTGCAAG TGGTACAAAA AGCCGAGCTA GAGTATTAAG TCTGGACAGT 
GGCACTGTAG TTAGTTTGAG TGATGCCAAC AGACTGGCCG CTCCTGAAAA TATAAAAACC 
TTAACCACTT CAAAATCAGA TCTAGAAGCC AAAGAAGGCG AAGTGCTAGA TCAGATGTCT 
CTGTTGGGGC GAGCTTCACA GTTAGAATCT GTAACACGGT CAAGAAATAG TTTGCCCAGC 
CAGGTTACTT TCCAAGATGT TGGAGAGCGA GACGCATCTG TTGGAGGTAA ATTTACCCCA 
TCTCTATATG AGACTGGAGG TTGTGACATG TCACTTGTAA ACTTTGAGCC AGCTGCAAGA 
AGGACGTCTA ATAATCTTTG TGACACAGAT TCCCATGTAT CAAGTTCTAC CTCAGTTCGG 
TTCTACCCAC ATGATCTAAT CCGATTAAAC AGGCTGCTAA CCATAGATAC AGATCTGTTA 
GAGCAGCAGG ATATTGATCT AAGCCCTGAC CTGCAGGACC AGATGCAACC ACAGGAACAG 
GCTGCCCAAA AAGTAAAACA CTACTACCGG TTTTGGATTC TCCCAAAGCT GTGGGTCGGC 
ATCAACTTTG ACAGGCTTAC ACTCTTAGCC TTGTTCGATA GGAACCGCGA GGTCCTAGAA 
AATGTGTTGT CTGTCATTCT CGCCATTCTA GTTGCCTTTT TGGGCTCTGT TCTTCTCATA 
GAAGGCTTCT TTAAAGATAT CTGGGTCTTC CAGTTTTGTC TTGTTATAGC CAGCTGTCAA 
TACTCTCTCC TTAAGAGTGT TCAACCAGAT TCTTCTTCCC CAAGACATGG CCATAATCGT 
ATCATTGCCT ATAGTAGGCC CGTTTACTTC TGTGCCTGTT GCGGCCTTAT TTGGTTATTG 
GATTATGGAA GCAAAAACAT GTCTACCACC AAATACAAAC TATATGGAAT GGCCTTCACC 
AATCCTCTGC TTCTAGTGTC AGCCAGGGAC TTAGTCATTG TCTTCACACT CTGTTTCCCA 
GTCGTGTTCT TTATTGGTCT CCTGCCTCAG GTGAACACAT TCGTCATGTA CCTGTGTGAG 
CAAGTAGATA TACATGTGTT TGGTGGAAAT GCTACCACCA GCTTGCTTGC AGCAATTTAC 
AGCTTTATCC GTAGCATTGT GGCCATAGCT TTACTCTACG GGCTGTGTTA CGGTGCTCTT 
AAGGATCCAT GGGATGCACA ACACATTCCT GTATTATTCT CTATTTTCTG TGGTGTTCTT 
GTGGCAGTTT CATACCATCT CAGCCGACAG AGCAGCGATC CCTCTGTCCT CATCTCACTG 
GTGCAATCAA AGATTTTTCC AAACAGCGAG GATACAAATC CTGAAGACCC ACTATCAGAA 
GTGAAAGACC CTCTTCCAGA AAAACTGAGA AGCTCTGTTA ATGAACGACT TCAGTCAGAC 
GTAGTAGTCT GTGTCGTTAT TGCAGTGTTG TACTTTGCAA TTCATGTCAG CACCGTATTT 
ACTGCATTAC AGCCGGTTCT GAGCTACGTA CTCTATGCAC TAGTTGGTTC TGTTGGACTC 
CTTACTCACT ACATTCTACC ACAAGTACGG AAACAGCTTC CTTGGCACTG CTTCTCCCAC 
CCGCTGCTGA AAACTAAGGA ATATTACCAA TTTGAAGTTC GAAATGCTGC TCATCTGATG 
TGGTTTGAGA AGGCTCACAT CTGGCTGCTT TTTATCGAGA AGAACATTAT TTATCCTCTT 
ATAGTTCTCA ATGAGCTTAG CATTGGTGCT AAGGATATCG TCAGCCCGAA AAAGCTGGAT 
GCAGAAGTTG GTGCATTGTT GATCACCATT GCCGGAATGA AGCTGCTGAG GTCGTCATTT 
AGCAGTCCCA CCTGCCAGTA TGCCACTGTC ATTTTCACTG TCCTCTTCTT TACTTTTGAC 
TACAAGGAGT ATACAGAAAA ACTGCTGCTA GATCTGTTCT TTATGTCAAT CCTCTTTAGC 
AAGCTCTGGG AACTGTTTTT CAAACTGCGC TTTGTCTACA CCTATATTGC CCCTTGGCAG 
ATCACGTGGG GATCAGCATT CCATGCTTTC GCACAGCCAT TTGCTGTACC TCATTCTGCA 
ATGCTGTTTG TACAGGCAAT TGTTTCTGCA CTGTTCTCCA CTCCCCTGAA CCCTTTCTTA 
GGGAGTGCAA TCTTTATTAC ATCATATGTA CGCCCTGTCA AGTTTTGGGA GAGAGACTAC 
AATACAAAGA GAGTTGACCA CTCAAACACA AGATTAGCCT CTCAGCTTGA CAGAAATCCA 
GGTTCAGATG ACAACAACTT GAATTCCATA TTTTATGAGC ATCTCACACG CTCCCTTCAG 
CATAGTCTGT GTGGTGATCT GTTGCTTGGC CGCTGGGGAA ATTACAATAC TGGGGATTGC 
TTTATTCTGG CCTCAGATTA TCTAAACGCC TTGGTGCACC TTATCGAAAT AGGCAATGGT 
CTGGTCACCT TCCAGCTAAG AGGACTAGAG TTCAGAGGTA CCTACTGCCA GCAGCGTGAA 
GTTGAAGCAA TCACTGAGGG TGTAGAGGAA GATGAAGGCT TTTGCTGTTG CGAACCTGGA 
CATATTCCTC ATATGCTGTC TTTCAATGCT GCCTTTGGGC AGCGCTGGCT GGCATGGGAA 
GTCTTGGTTA CAAAGTATGT TCTGGAAGGT TACAGCATCA CAGACAACAG TGCAGCCTCT 
ATGCTTCAAG TGTTTGACCT TCGCAGGATC CTCACTACCT ATTATGTTAA GGGCATTATA 
TACTATGTGA CAGCATCTGC AAAACTGGAG GAGTGGTTAG CAAATGAGAC AATGCAGGAA 
GGTCTTCGCC AGTGTTCTGA TCATTGTTAT GTTGATGTAG ATCCAACTTT CAACCCAAAT 
ATTGATGAAG ACTATGACCT TCGCTTAGCA GGCATTTCAC GGGAGAGCTT CTGTGCAAGC 
TATCTTAACT GGATTAAGTA CTGTTCCTCT AGAAGGGAGA AGCCTCTTGA TGCTGAAAAG 
GATTCACCTT TGGTAACACT TTGTTATGGG CTCTGTATTT TGGGGAGAAG GGCACTTGGT 
ACAGCTTCTC ATCACATGTC CAGCAATTTG GAGTCCTTTC TATATGGTTT ACATGCCTTG 
TTTAAAGGAG ATTTCCGCAT ATCATCTCTC CGTGATGAGT GGATTTTTGC TGATATGGAG 
CTTTTAAGAA AAGTAGTAGT TCCTGGCATA CGTATGTCAC TAAAACTTCA TCAGGACCAC 
TTTACATCTC CAGATGAGTA TGATGATCCT GCAGTTTTGT ATGAAGCCAT TACATCACAT 
GAGCAGAACC TTGTTATTGC GCATGAGGGA GATCCAGCGT GGAGGAGTGC TGTTCTTTCT 
AACTCACCTT CTCTTCTCGC GCTGCGTCAT GTGATGGATG ATGGAACCAA CGAATACAAG 
ATCATAATGT TAAATAAACG TTATCTTAGT TTTAGAGTCA TTAAGGTAAA CAAGGAGTGT 
GTGCGGGGCT TATGGGCTGG TCAGCAGCAA GAATTGGTCT TTCTTCGTAA TCGGAACCCT 
GAAAGAGGCA GCATCCAAAA TGCAAAGCAG GCTCTCAGGA ATATGATCAA TTCATCATGT 
GACCAACCTA TTGGATACCC AATTTATGTT TCTCCACTGA CAACCTCTTA TTCAGACAGC 
CATGATCAGC TTAAACAGAT ATTTGGTGGG CCAATTAGCT TGGCCAATAT CCGGAACTTC 
ATTGTATCTA CATGGCACAG ATTAAGAAAA GGTTGCGGTG CAGGATGCAA TAGTGGTGGA 
AATATTGAGG ACTCTGACAC TGGAGGCGGG ACCTCCATTA ATAGCAACAA TCCACCAAAT 
GCAAATGAAA CTCAGAGCAG TCTGTCACAA GTTGGTGTCC CAGGTGGAAC AGGGCATGTG 
CCGAATGCAC GGCTCCAGCC TCCGGGGATG CCTCATCCAT CAACACTAGG AACCAGTCAC 
AGTTCTCACT CCATGCAGTC AAGCCTTGTC AGACATTCCC CAGCTCGTGG ATCAGTAGCA 
AGTCAATCAT CTTACTGCTA CAACAGCCGT CACTCATCAC TTAGAACATC CACAACAGGA 
TTTGTGCCTT GTAGGCGCTC CTCTACCAGC CAGATATCTC TTCGCAATCT CCCCTCATCC 
ATTCAGTCCC GTCTTTCCAT GGTGAACCAA ACAGACCCCA CAGGACAGTC AGTTTTGGTT 
GGCCTTCCTT CCTCCAGCAG CTCAAGCCAG AGCATCCCTG CATGCAAAAG ACACACCTTA 
GTGGGCTTTT TAGGAACAGA AGGAGGCAAT AACCTCACTG AAACGCAATC CTGTGGCAAT 
TCTAGTCCAG CAACCCATTC AAGAGCCAAA AAGGAAGAGG TTGTTTACAG AGTCCAGATT 
GTGGATCCAA GCCAAGTGCT TGAAGGCGTG AATATATCCA AAAGAAAGGA GCTGCAGTGG 
CCAGATGAGC TGGTGCGGCT AAAAGCCGGC AAGAACAGCT GGAAAGACTG GAGCCCTCAG 
GAAGGCATGG AAGGCCATGT AATTCACCGC TGGGTGCCTT GCAGCAGAGA CCCTTGTAAT 
AGGTCCCACA TTGACAAAAC AATCCTGCTG ATTAAGATTG AGGACAAATA TGTTGCTGTG 
ATTGAAACTG GTGTGCTTGA ACTCGGAGCT GAAGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012824455.1
CDS	272..6910
Translation	MGSQTLQILRQGVWASITGGWYYDPHQSTFVNTLHLYIWLFLLCFPFTLYMALPTSLVIVAVYCPVVAAVFIVLKLVNYRLHRALDEGEIVERSASELTERVAKAEHSDSSARREDSNGPGDPGGGIEMSEFVREATPPVGCSSRNSYSGLDPSNQVGVSRYGGASGSKGDTDAAKTSDDISLSLGQSYSLCKEASEDQDLTVDQKLFRLVSNDSFVSMQPSISSGGKDLTRDFSDKGSIPTHSHHHHVDLSLSNACDTELASLVPLHSHSYRKDHRPRGVPRTSSSAVAFPDTSLTDFSLYQPRRGLDPVSELEASKPHSGSKDSLVHNACASGIFQLDCLKSKGAQQLSRSGSVKHMKTDKSMDSLRSLSTRSSGSTESYCSGTDRDTNSTVSSYKSEQTSSTHVESILSEHEDVTAAKRVHCSDLEEEHSSATDKRTSSIRTALERNTAQEAKDANTSDDVQSPRGLSSSASEEANKNPHANELTVQTDWLPGKLSVNIDEPGEKPVILVDLRSLKETGIKQRDNDVRPKSSSLIHRTSSAHKSCRRRTGKKRASSFDASRHKEYVSFQGIADTKPHSTGFCHDEDSSDQSDLSRTSSVQSAHRFSSDSSSSTTSHSCQSPEARYSALRTKYRIKERGTDSHHTHLNCEGTSKKRSNRRTSSASGTKSRARVLSLDSGTVVSLSDANRLAAPENIKTLTTSKSDLEAKEGEVLDQMSLLGRASQLESVTRSRNSLPSQVTFQDVGERDASVGGKFTPSLYETGGCDMSLVNFEPAARRTSNNLCDTDSHVSSSTSVRFYPHDLIRLNRLLTIDTDLLEQQDIDLSPDLQDQMQPQEQAAQKVKHYYRFWILPKLWVGINFDRLTLLALFDRNREVLENVLSVILAILVAFLGSVLLIEGFFKDIWVFQFCLVIASCQYSLLKSVQPDSSSPRHGHNRIIAYSRPVYFCACCGLIWLLDYGSKNMSTTKYKLYGMAFTNPLLLVSARDLVIVFTLCFPVVFFIGLLPQVNTFVMYLCEQVDIHVFGGNATTSLLAAIYSFIRSIVAIALLYGLCYGALKDPWDAQHIPVLFSIFCGVLVAVSYHLSRQSSDPSVLISLVQSKIFPNSEDTNPEDPLSEVKDPLPEKLRSSVNERLQSDVVVCVVIAVLYFAIHVSTVFTALQPVLSYVLYALVGSVGLLTHYILPQVRKQLPWHCFSHPLLKTKEYYQFEVRNAAHLMWFEKAHIWLLFIEKNIIYPLIVLNELSIGAKDIVSPKKLDAEVGALLITIAGMKLLRSSFSSPTCQYATVIFTVLFFTFDYKEYTEKLLLDLFFMSILFSKLWELFFKLRFVYTYIAPWQITWGSAFHAFAQPFAVPHSAMLFVQAIVSALFSTPLNPFLGSAIFITSYVRPVKFWERDYNTKRVDHSNTRLASQLDRNPGSDDNNLNSIFYEHLTRSLQHSLCGDLLLGRWGNYNTGDCFILASDYLNALVHLIEIGNGLVTFQLRGLEFRGTYCQQREVEAITEGVEEDEGFCCCEPGHIPHMLSFNAAFGQRWLAWEVLVTKYVLEGYSITDNSAASMLQVFDLRRILTTYYVKGIIYYVTASAKLEEWLANETMQEGLRQCSDHCYVDVDPTFNPNIDEDYDLRLAGISRESFCASYLNWIKYCSSRREKPLDAEKDSPLVTLCYGLCILGRRALGTASHHMSSNLESFLYGLHALFKGDFRISSLRDEWIFADMELLRKVVVPGIRMSLKLHQDHFTSPDEYDDPAVLYEAITSHEQNLVIAHEGDPAWRSAVLSNSPSLLALRHVMDDGTNEYKIIMLNKRYLSFRVIKVNKECVRGLWAGQQQELVFLRNRNPERGSIQNAKQALRNMINSSCDQPIGYPIYVSPLTTSYSDSHDQLKQIFGGPISLANIRNFIVSTWHRLRKGCGAGCNSGGNIEDSDTGGGTSINSNNPPNANETQSSLSQVGVPGGTGHVPNARLQPPGMPHPSTLGTSHSSHSMQSSLVRHSPARGSVASQSSYCYNSRHSSLRTSTTGFVPCRRSSTSQISLRNLPSSIQSRLSMVNQTDPTGQSVLVGLPSSSSSSQSIPACKRHTLVGFLGTEGGNNLTETQSCGNSSPATHSRAKKEEVVYRVQIVDPSQVLEGVNISKRKELQWPDELVRLKAGKNSWKDWSPQEGMEGHVIHRWVPCSRDPCNRSHIDKTILLIKIEDKYVAVIETGVLELGAEV

Target ORF information:

RefSeq Version	XM_012969001.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pecanex homolog (pcnx), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012969001.2

ORF Insert Sequence:

ATGGGTTCCC AGACACTGCA AATCCTCCGG CAGGGAGTCT GGGCTTCCAT CACCGGAGGC 
TGGTACTACG ACCCCCACCA GAGTACCTTT GTCAATACCC TACACCTCTA CATCTGGCTC 
TTCCTCCTCT GCTTCCCCTT CACCCTCTAC ATGGCACTAC CCACATCTTT GGTGATTGTT 
GCTGTGTACT GCCCAGTCGT TGCAGCTGTT TTTATTGTTT TGAAGCTTGT TAACTACCGT 
CTGCACAGGG CATTGGATGA AGGAGAAATT GTGGAGAGGA GTGCTAGTGA GTTGACTGAG 
AGGGTGGCAA AGGCAGAACA CAGTGACAGC TCTGCAAGAA GAGAGGACAG CAATGGACCT 
GGTGATCCAG GTGGAGGGAT TGAAATGTCT GAATTTGTTC GAGAAGCAAC TCCTCCAGTT 
GGATGCAGTT CACGTAATTC CTACTCTGGG TTGGACCCAA GCAATCAGGT TGGTGTGTCT 
CGTTATGGAG GTGCATCTGG AAGCAAAGGG GATACAGATG CTGCAAAGAC ATCAGATGAT 
ATCAGTCTGA GTCTTGGCCA GAGTTACAGT TTGTGCAAAG AAGCAAGTGA AGACCAAGAT 
TTGACTGTGG ATCAGAAGCT TTTCCGTCTC GTCTCCAATG ACTCCTTTGT CTCCATGCAG 
CCATCTATAT CCTCGGGTGG AAAAGACTTA ACTAGAGACT TCAGTGACAA AGGATCAATC 
CCAACACACA GTCATCATCA CCATGTGGAT CTGTCTCTGT CTAATGCTTG TGACACAGAA 
TTGGCCTCAC TTGTTCCATT GCATTCACAT TCGTACAGGA AAGATCATCG ACCAAGAGGT 
GTCCCAAGGA CTTCAAGTTC TGCTGTTGCT TTCCCAGACA CTTCTCTCAC TGACTTTTCA 
CTCTATCAAC CAAGGCGCGG GCTGGATCCT GTTAGTGAAC TTGAAGCTTC CAAACCACAT 
TCTGGGTCCA AAGATTCACT AGTACATAAT GCTTGTGCTT CAGGGATTTT TCAATTAGAC 
TGTCTAAAAA GCAAAGGAGC CCAGCAGCTT TCAAGAAGTG GTAGTGTTAA ACACATGAAG 
ACTGACAAAA GCATGGATAG TTTAAGAAGC CTCAGCACGA GAAGCAGTGG ATCCACAGAA 
AGTTATTGTA GTGGAACAGA TCGGGACACA AATAGCACTG TTAGCAGCTA CAAAAGTGAA 
CAGACCAGTT CAACTCATGT TGAAAGTATT TTATCTGAGC ATGAGGATGT TACTGCTGCA 
AAGAGAGTCC ATTGTAGTGA CCTGGAGGAA GAACATAGCT CTGCTACTGA TAAAAGGACT 
AGTAGTATAA GAACTGCTTT AGAAAGAAAC ACTGCTCAGG AAGCCAAGGA TGCCAACACA 
TCTGATGATG TACAAAGTCC AAGGGGTCTT AGCTCATCAG CATCAGAGGA AGCTAATAAG 
AATCCACACG CAAATGAGTT AACTGTACAA ACAGACTGGC TTCCAGGTAA ACTGTCAGTG 
AACATTGATG AACCTGGGGA GAAACCAGTC ATTTTAGTTG ACTTGAGATC TTTGAAAGAA 
ACGGGTATTA AGCAGAGAGA CAATGACGTA CGACCCAAAT CATCTAGTTT AATACATCGA 
ACATCCTCTG CTCATAAATC ATGCAGGAGA CGGACAGGTA AAAAAAGAGC CAGCAGTTTT 
GATGCAAGTA GACACAAAGA ATATGTTTCC TTTCAAGGTA TAGCTGATAC CAAACCTCAC 
AGTACTGGCT TTTGTCATGA TGAAGATTCT AGCGACCAAA GCGACTTGAG CAGGACCTCA 
AGCGTTCAGT CGGCCCACCG TTTCAGCAGC GATAGCTCTT CCAGCACTAC TTCCCATTCA 
TGCCAGTCTC CTGAAGCAAG ATACAGTGCC CTAAGGACAA AATATCGAAT AAAAGAAAGA 
GGCACAGATT CACATCACAC ACACCTAAAT TGTGAAGGAA CTAGTAAAAA GCGGTCCAAC 
CGCAGGACTT CTAGTGCAAG TGGTACAAAA AGCCGAGCTA GAGTATTAAG TCTGGACAGT 
GGCACTGTAG TTAGTTTGAG TGATGCCAAC AGACTGGCCG CTCCTGAAAA TATAAAAACC 
TTAACCACTT CAAAATCAGA TCTAGAAGCC AAAGAAGGCG AAGTGCTAGA TCAGATGTCT 
CTGTTGGGGC GAGCTTCACA GTTAGAATCT GTAACACGGT CAAGAAATAG TTTGCCCAGC 
CAGGTTACTT TCCAAGATGT TGGAGAGCGA GACGCATCTG TTGGAGGTAA ATTTACCCCA 
TCTCTATATG AGACTGGAGG TTGTGACATG TCACTTGTAA ACTTTGAGCC AGCTGCAAGA 
AGGACGTCTA ATAATCTTTG TGACACAGAT TCCCATGTAT CAAGTTCTAC CTCAGTTCGG 
TTCTACCCAC ATGATCTAAT CCGATTAAAC AGGCTGCTAA CCATAGATAC AGATCTGTTA 
GAGCAGCAGG ATATTGATCT AAGCCCTGAC CTGCAGGACC AGATGCAACC ACAGGAACAG 
GCTGCCCAAA AAGTAAAACA CTACTACCGG TTTTGGATTC TCCCAAAGCT GTGGGTCGGC 
ATCAACTTTG ACAGGCTTAC ACTCTTAGCC TTGTTCGATA GGAACCGCGA GGTCCTAGAA 
AATGTGTTGT CTGTCATTCT CGCCATTCTA GTTGCCTTTT TGGGCTCTGT TCTTCTCATA 
GAAGGCTTCT TTAAAGATAT CTGGGTCTTC CAGTTTTGTC TTGTTATAGC CAGCTGTCAA 
TACTCTCTCC TTAAGAGTGT TCAACCAGAT TCTTCTTCCC CAAGACATGG CCATAATCGT 
ATCATTGCCT ATAGTAGGCC CGTTTACTTC TGTGCCTGTT GCGGCCTTAT TTGGTTATTG 
GATTATGGAA GCAAAAACAT GTCTACCACC AAATACAAAC TATATGGAAT GGCCTTCACC 
AATCCTCTGC TTCTAGTGTC AGCCAGGGAC TTAGTCATTG TCTTCACACT CTGTTTCCCA 
GTCGTGTTCT TTATTGGTCT CCTGCCTCAG GTGAACACAT TCGTCATGTA CCTGTGTGAG 
CAAGTAGATA TACATGTGTT TGGTGGAAAT GCTACCACCA GCTTGCTTGC AGCAATTTAC 
AGCTTTATCC GTAGCATTGT GGCCATAGCT TTACTCTACG GGCTGTGTTA CGGTGCTCTT 
AAGGATCCAT GGGATGCACA ACACATTCCT GTATTATTCT CTATTTTCTG TGGTGTTCTT 
GTGGCAGTTT CATACCATCT CAGCCGACAG AGCAGCGATC CCTCTGTCCT CATCTCACTG 
GTGCAATCAA AGATTTTTCC AAACAGCGAG GATACAAATC CTGAAGACCC ACTATCAGAA 
GTGAAAGACC CTCTTCCAGA AAAACTGAGA AGCTCTGTTA ATGAACGACT TCAGTCAGAC 
GTAGTAGTCT GTGTCGTTAT TGCAGTGTTG TACTTTGCAA TTCATGTCAG CACCGTATTT 
ACTGCATTAC AGCCGGTTCT GAGCTACGTA CTCTATGCAC TAGTTGGTTC TGTTGGACTC 
CTTACTCACT ACATTCTACC ACAAGTACGG AAACAGCTTC CTTGGCACTG CTTCTCCCAC 
CCGCTGCTGA AAACTAAGGA ATATTACCAA TTTGAAGTTC GAAATGCTGC TCATCTGATG 
TGGTTTGAGA AGGCTCACAT CTGGCTGCTT TTTATCGAGA AGAACATTAT TTATCCTCTT 
ATAGTTCTCA ATGAGCTTAG CATTGGTGCT AAGGATATCG TCAGCCCGAA AAAGCTGGAT 
GCAGAAGTTG GTGCATTGTT GATCACCATT GCCGGAATGA AGCTGCTGAG GTCGTCATTT 
AGCAGTCCCA CCTGCCAGTA TGCCACTGTC ATTTTCACTG TCCTCTTCTT TACTTTTGAC 
TACAAGGAGT ATACAGAAAA ACTGCTGCTA GATCTGTTCT TTATGTCAAT CCTCTTTAGC 
AAGCTCTGGG AACTGTTTTT CAAACTGCGC TTTGTCTACA CCTATATTGC CCCTTGGCAG 
ATCACGTGGG GATCAGCATT CCATGCTTTC GCACAGCCAT TTGCTGTACC TCATTCTGCA 
ATGCTGTTTG TACAGGCAAT TGTTTCTGCA CTGTTCTCCA CTCCCCTGAA CCCTTTCTTA 
GGGAGTGCAA TCTTTATTAC ATCATATGTA CGCCCTGTCA AGTTTTGGGA GAGAGACTAC 
AATACAAAGA GAGTTGACCA CTCAAACACA AGATTAGCCT CTCAGCTTGA CAGAAATCCA 
GGTTCAGATG ACAACAACTT GAATTCCATA TTTTATGAGC ATCTCACACG CTCCCTTCAG 
CATAGTCTGT GTGGTGATCT GTTGCTTGGC CGCTGGGGAA ATTACAATAC TGGGGATTGC 
TTTATTCTGG CCTCAGATTA TCTAAACGCC TTGGTGCACC TTATCGAAAT AGGCAATGGT 
CTGGTCACCT TCCAGCTAAG AGGACTAGAG TTCAGAGGTA CCTACTGCCA GCAGCGTGAA 
GTTGAAGCAA TCACTGAGGG TGTAGAGGAA GATGAAGGCT TTTGCTGTTG CGAACCTGGA 
CATATTCCTC ATATGCTGTC TTTCAATGCT GCCTTTGGGC AGCGCTGGCT GGCATGGGAA 
GTCTTGGTTA CAAAGTATGT TCTGGAAGGT TACAGCATCA CAGACAACAG TGCAGCCTCT 
ATGCTTCAAG TGTTTGACCT TCGCAGGATC CTCACTACCT ATTATGTTAA GGGCATTATA 
TACTATGTGA CAGCATCTGC AAAACTGGAG GAGTGGTTAG CAAATGAGAC AATGCAGGAA 
GGTCTTCGCC AGTGTTCTGA TCATTGTTAT GTTGATGTAG ATCCAACTTT CAACCCAAAT 
ATTGATGAAG ACTATGACCT TCGCTTAGCA GGCATTTCAC GGGAGAGCTT CTGTGCAAGC 
TATCTTAACT GGATTAAGTA CTGTTCCTCT AGAAGGGAGA AGCCTCTTGA TGCTGAAAAG 
GATTCACCTT TGGTAACACT TTGTTATGGG CTCTGTATTT TGGGGAGAAG GGCACTTGGT 
ACAGCTTCTC ATCACATGTC CAGCAATTTG GAGTCCTTTC TATATGGTTT ACATGCCTTG 
TTTAAAGGAG ATTTCCGCAT ATCATCTCTC CGTGATGAGT GGATTTTTGC TGATATGGAG 
CTTTTAAGAA AAGTAGTAGT TCCTGGCATA CGTATGTCAC TAAAACTTCA TCAGGACCAC 
TTTACATCTC CAGATGAGTA TGATGATCCT GCAGTTTTGT ATGAAGCCAT TACATCACAT 
GAGCAGAACC TTGTTATTGC GCATGAGGGA GATCCAGCGT GGAGGAGTGC TGTTCTTTCT 
AACTCACCTT CTCTTCTCGC GCTGCGTCAT GTGATGGATG ATGGAACCAA CGAATACAAG 
ATCATAATGT TAAATAAACG TTATCTTAGT TTTAGAGTCA TTAAGGTAAA CAAGGAGTGT 
GTGCGGGGCT TATGGGCTGG TCAGCAGCAA GAATTGGTCT TTCTTCGTAA TCGGAACCCT 
GAAAGAGGCA GCATCCAAAA TGCAAAGCAG GCTCTCAGGA ATATGATCAA TTCATCATGT 
GACCAACCTA TTGGATACCC AATTTATGTT TCTCCACTGA CAACCTCTTA TTCAGACAGC 
CATGATCAGC TTAAACAGAT ATTTGGTGGG CCAATTAGCT TGGCCAATAT CCGGAACTTC 
ATTGTATCTA CATGGCACAG ATTAAGAAAA GGTTGCGGTG CAGGATGCAA TAGTGGTGGA 
AATATTGAGG ACTCTGACAC TGGAGGCGGG ACCTCCATTA ATAGCAACAA TCCACCAAAT 
GCAAATGAAA CTCAGAGCAG TCTGTCACAA GTTGGTGTCC CAGGTGGAAC AGGGCATGTG 
CCGAATGCAC GGCTCCAGCC TCCGGGGATG CCTCATCCAT CAACACTAGG AACCAGTCAC 
AGTTCTCACT CCATGCAGTC AAGCCTTGTC AGACATTCCC CAGCTCGTGG ATCAGTAGCA 
AGTCAATCAT CTTACTGCTA CAACAGCCGT CACTCATCAC TTAGAACATC CACAACAGGA 
TTTGTGCCTT GTAGGCGCTC CTCTACCAGC CAGATATCTC TTCGCAATCT CCCCTCATCC 
ATTCAGTCCC GTCTTTCCAT GGTGAACCAA ACAGACCCCA CAGGACAGTC AGTTTTGGTT 
GGCCTTCCTT CCTCCAGCAG CTCAAGCCAG AGCATCCCTG CATGCAAAAG ACACACCTTA 
GTGGGCTTTT TAGGAACAGA AGGAGGCAAT AACCTCACTG AAACGCAATC CTGTGGCAAT 
TCTAGTCCAG CAACCCATTC AAGAGCCAAA AAGGAAGAGG TTGTTTACAG AGTCCAGATT 
GTGGATCCAA GCCAAGTGCT TGAAGGCGTG AATATATCCA AAAGAAAGGA GCTGCAGTGG 
CCAGATGAGC TGGTGCGGCT AAAAGCCGGC AAGAACAGCT GGAAAGACTG GAGCCCTCAG 
GAAGGCATGG AAGGCCATGT AATTCACCGC TGGGTGCCTT GCAGCAGAGA CCCTTGTAAT 
AGGTCCCACA TTGACAAAAC AATCCTGCTG ATTAAGATTG AGGACAAATA TGTTGCTGTG 
ATTGAAACTG GTGTGCTTGA ACTCGGAGCT GAAGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	pcnx1
Entrez Gene ID	100135121
Full Name	pecanex homolog 1
Synonyms	PCNXL1,pcnx,pecanex
General protein information	Preferred Names pecanex homolog 1 Names pecanex-like protein 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

	PCNX	XP_001084081.1
Homo sapiens (human)	PCNX	NP_055797.2
Mus musculus (house mouse)	Pcnx	NP_061284.2
Pan troglodytes (chimpanzee)	PCNX	XP_510039.3
Canis lupus familiaris (dog)	PCNX	XP_005623651.1
Bos taurus (cattle)	PCNX	XP_002691096.2
Rattus norvegicus (Norway rat)	Pcnx	NP_001163818.1
Gallus gallus (chicken)	PCNX	XP_423893.4
Danio rerio (zebrafish)	pcnx	XP_005157080.1
Xenopus tropicalis (tropical clawed frog)	LOC100135121	XP_004917311.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

pcnx1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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