gbf1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

  • Gene

  • Clones

  • gRNAs

The following gbf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gbf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa62534 XM_031906081.1
Latest version!
Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa62534 XM_031906082.1
Latest version!
Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa62534 XM_031906080.1
Latest version!
Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa62535 XM_031906083.1
Latest version!
Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa62536 XM_031906084.1
Latest version!
Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa62537 XM_031906085.1
Latest version!
Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa62538 XM_012966851.3
Latest version!
Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa62538 XM_012966852.3
Latest version!
Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa62539 XM_031906086.1
Latest version!
Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa62540 XM_018095873.2
Latest version!
Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa62541 XM_018095871.2
Latest version!
Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa12833 XM_018095871.1
Latest version!
Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OXa12834 XM_018095872.1
Latest version!
Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OXa12835 XM_018095873.1
Latest version!
Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OXa33097 XM_012966854.2
Latest version!
Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OXa33097 XM_012966852.2
Latest version!
Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OXa33097 XM_012966853.2
Latest version!
Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OXa33097 XM_012966851.2
Latest version!
Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa62534
    Clone ID Related Accession (Same CDS sequence) XM_031906080.1 , XM_031906081.1 , XM_031906082.1
    Accession Version XM_031906080.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5673bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGA TCTCTCCATG TCTACTCTGC
    AAAATGGAGC TTAATAGTGG TGAACAGTCC AAAAACCTGA ACCAGTTAGA GAGGGTACTT
    CTTTTTAAGA GCCTGAAGTT GAAAATGAGA GCTGGAGGAA TGAGTGACTC CTCAAAATGG
    AAAAAGCAGA AGAAATCTCC TAGGCAGCCA CGTCATGTTA CCAAAGCTAT GGCGGCAGCA
    GAACCCTCCT CTCAGCAACC AGTAGTTAAT GGCACCACAA TTACTACTAA CCTCACTGGT
    GAAGTTCCTT TTATTGATGC TTCCTCTCCA GATGCTAATT TTCCCATTTC AGGGGTGAGT
    TCAGAGGCCA CGTCTGCTGT AGTCAGTCCT ACCACAGATA GTGGTGTAGA TATGTCCTCT
    CAGGCAACAT CTAAAGAAGA CCTTTCTGAA CTGGATCCTG CAAGTCCATC TCTTTCTGCC
    ATTCAGCAAT CAGGAGACAT TTGTCTTTCT GAACAGCAAA ACCAGGATGA TGATAAGATT
    GAAGACCTTA TGGAAAGGCA TACATATTCT GCTTCTGTGG AATCCATACC AGAAGTATTA
    GATGATAATG TGTCATTGGC TGATCAATCA GACTCCGCTT CAGTCCATGA TATGGATTAT
    GTAAATCCTA GAGGTGTGAG ATTTACCCAA TCTTCACAGA AAGAAGGAAC AGTCTTAGTA
    CCATATGGAC TGCCATGCAT TCGGGAATTA TTTCGTTTTC TTATTTCTTT AACCAACCCC
    CATGATCGAC ATAACTCCGA AGTGATGATA CACATGGGTT TACAGTTGAT CACAGTTGCT
    CTTGAGTCTG CACCTATTGA AAATTATGTT TCGCTTTTAG GCCTTGTAAA GGATGAACTT
    TGTCGACACT TATTTCAGCT ACTGAGCATT GAGAGACTGA ACCTATATGC AGCTTCCTTG
    CGAGCATGTT TTCTTTTGTT TGAGGGTATG AGGGAGCATT TGAAGTTCCA GCTAGAGATG
    TACATAAAGA AGCTCATGGA TATTATTACT GTGGAGAACC CTAAGATGCC TTATGAGATG
    AAAGAAATGG CTCTTGAAGC TATTGTGCAA TTGTGGAGAA TCCCGAGCTT TGTTACAGAA
    TTATACATTA ATTACGACTG TGATTATTAT TGCTCCAACC TTTTTGAAGA TCTCACTAAA
    CTGCTTTCCA AGAATGCCTT TCCAGTTTCT GGACAGCTGT ACACCACTCA TCTCCTTTCC
    CTGGAAGCTC TGCTAACTGT CATTGATAGC ACAGAATCAC ACTGCCAGGC TAAAAATCTA
    AGCAATGTGT CACAGGATAA GAAAGAAACT GGAAAACCAA GTGGAGATCT GTCAGAAGGG
    ACCAAAGATT CTGGAAATCT CAATGAACAC TCCATTGAAG TTAAGCCATT CACTGTTAGT
    GCAGAAAACA ATGGATCGAA GCCCCCCACA AGTGGCCACT TAATGGCAGA TAAAATGACT
    CTTGGGGTCC AAGAAACAGA GTACAGCACT GATGGAGGTG AGAAGAAGAC TTTAAAGAAG
    CCCCAACGCT TTTCCTGCTT ACTTCCGAAT GTTCAAGAGC TGAATGACAT TAAGACCAAA
    AAGAAGCTTC TTATAACTGG AACTGAGCAG TTCAACCAGA AACCAAAGAA AGGAGTGCAG
    TTTCTTCAGG AAAAAGACCT TTTGGCAACC CCCATGGATA ATGCTGAGGT AGCACAGTGG
    CTCAGAGAAA ATCCAAGGCT GGACAAGAAA ATGATTGGGG AGTTTGTGAG TGATCGCAAA
    AATCTGGACT TACTTGAAAG TTTTGTAGGA ACCTTCCATT TCCAAGGCCT GAGAGTTGAT
    GAAGCTTTAA GGCTTTACCT TGAAGCTTTT CGTTTGCCTG GCGAGGCACC TGTGATTCAG
    AGGTTACTGG AGGCTTTTAC AGAGCACTGG CGAAAATCCA ATGGAACTCC TTTTGCACAC
    AGTGATGCCT GCTTTGCTTT GGCTTATGCA GTTATCATGC TGAACACAGA CCAACACAAT
    CACAATGTCC GCAAGCAAAA TGTGCCAATG ACTTTAGAGG AATTCAGGAA AAATTTAAAG
    GGTGTTAATG GTGGAAAAGA TTTTGACCAA GATATGCTAG AGGATATTTA TCACGCAATC
    AAAAATGAAG AAATTGTAAT GCCTGAAGAG CAGACTGGCT TAGTAAAAGA GAATTATTTT
    TGGAATGTTT TGCTGCATCG GGGAGCCACT CCAGAAGGAA TGTTCTTGCA TGTGGATCCT
    GGAAGTTATG ACCATGACCT CTTTACTATG ACTTGGGGGC CAACAATAGC TGCCTTGTCC
    TATGTGTTTG ATAAGAGCAT GGATGAAACG ATTATTCAGA AAGCAATATC TGGTTTTAGG
    AAATGTGCAA TGATATCTGC TCACTATGGT CTCAGTGATG TTTTTGACAA TCTCATAATT
    TCCTTGTGCA AGTTTACAAC ACTTAGCAGT GAAGCCGTTG AAAATCTTCC CACTGTTTTT
    GGAAGCAACC TAAAAGCTCA GATCGCTGCA AAGACTGTGT TCCACTTATC CCACAGACAT
    GGTGACATCC TTCGAGAGGG ATGGAAAAAT ATTATGGACT CCATGCTGCA GCTTTTTAGA
    GCTGAGCTGC TTCCCAAGGC TATGGTTGAG GTTGAAGATT TTGTAGACCC CAATGGAAAA
    ATATCTCTTC AACGTGAGGA AATCCCTGCC AACCGTGGGG AATCCACTGT ACTAAGTTTT
    GTCAGCTGGC TGACTCTGAG TGGGACAGAA CAGTCAAGTC TAAGAGGACC TTCTACTGAA
    AATCAAGAGG CCAAGAAACT AGCGCTGGAA TGTATCAAGT TATGTGATCC AGAGAAACTG
    ATCACAGAGA GCAAGTTTCT TCAACTGGAG TCTTTGCAAG AACTAATGAA GGCTCTGATA
    TCTGTGACAC CAGATGAAGA AACGTATGAT GAAGAAGATG CTGCATTCTG CCTAGAAATG
    TTAATGAGAA TTGTGCTAGA AAACAGAGAT CGTGTTGGCT GTGTTTGGCA GGCTGTACGA
    GATCATCTCT ATCGTCTATG TGTCCATGCT GTAGAGTTCT GCTTCCTGGT TGAAAGAGCA
    GTTGTAGGGC TGCTGCGCTT GGCAATACGC CTTTTACGCA GAGAGGAAAT TAGTGGTCAG
    GTTATCCTCT CCTTGAGAAT TCTTTTAATG ATGAAACCAA CTGTGTTGTC TAAAGTGAGT
    CGCCAAGTTG CTTTTGGTGT GCATGAGCTC TTGAAAACCA ATGCTGCTAA CATCCATTCC
    AGCAATGACT GGTACACACT ATTCTGCCTT TTGGAGTGCA TTGGGGCAGG AGTGAAGCCG
    CCCCCTGCCT TACAGGTGAC GACGAGAGCT GACAATGACA CAGGTGCGCA GTCTGACAGT
    GAAGTTTCCT CATACCATCC AAGTGAAGTG AATCTTGATC GTGGATACAC CTCAGACTCT
    GAGGTATATG CAGAACACAG CAAGCAGTCA AAAATGCACC GCTCTGTGAC TGATGTGGAT
    ATGATGAACA GTGGCTGGCT GATGGTTGGA AAGGAAGATG TTGACTCTTC TAAAGTTGCT
    ACAGTTATTA ACAAACCTGT TGCTAATGCC CCAGTGAATC AATACAGCCT AACCATTGGT
    TTGGATTTTG GCCCTCACGA TACCAAATCT CTCATGAAGA GCGTGGAGTC CCTTTCTTTT
    ATTGTTCGAG ATGCTGCACA TGTCACACCA GAAAACTTTG AACTCTGTGT TAAAACAATC
    CGTGTCTTTG TAGAGGCAAG TCTAAATGGA GGTGTGTGGT ACAAATCTCA GGAGAAAAGG
    GTAAAGAACC ACAAATATGA CAGTAAATCC AACCGCTTCA AAAAGAAAAC CAAGGAAAAA
    GAAAATTCTG TCCGCCGTAC ACGGGCTTCA AATCAGCGAC AATACCGATC TCACAGTGAT
    GATGAGGAAG ATGAAAGTGT ACCAGCCAGC TATCACACTG TGTCATTACA GGTTAGTCAT
    GATCTTTTGG ACCTAATGCA CACACTTCAC ACAAGAGCTG CCACCATATA CAGTTCTTGG
    GCAGAGGAAC AGCGCAACCT AGAGACAGCT GCTGAGAAAA TTACAGCCGA TTCTAAGACA
    CTGTGGTCAA ACTGTTGGTG TCCTTTACTG CAAGGTATTG CTTGGCTCTG CTGTGATGCT
    CGGCGCCAGG TCAGAATGCA GGCTCTCACC TACCTGCAAA GGGCACTTTT AGTCCATGAC
    TTACAGGCTC TTGATGCACT TGAATGGGAA TCATGCTTTA ATAAGGTGCT ATTTCCATTG
    CTGACCAAAC TATTGGAAAA TATTAGTCCA GCTGATGTTG GCGGGATGGA GGAGACCAGA
    ATGAGAGGCT CCACTCTGCT CTCAAAGGTA TTTCTACAGC ATCTGTCTCC TCTTCTTTCT
    CTGTCGACAT TTGCTGCCTT GTGGCTTACT ATTCTTGATT TCATGGACAA GTACATGCAT
    GCTGGCTCCA GTGACTTACT GCCTGAGGCA ATTCCAGAGT CACTGAAAAA TATGCTTCTC
    GTAATGGACA CTGCCGGAAT ATTCCACAGT GCAGACTCGC GGACGGGCTA CTCTGATTTA
    TGGGAGATCA CGTGGGAAAG GATAGATTGC TTTTTACCTC GTCTAAGGGA AGAACTGTTT
    AAACAAACTG TGATACAAGA TCCACCCAAT ATAGTTACAA CAGAAACTCC TGTTGAGCCA
    AGACCTCCCC CTCAATCAAC TCAGTCAGAG AGACCAGCGG AGCAGGGACC TTGTGACTCA
    GAGACTCAAA GTTTAGCAAT CTCATCTGCT CCAGTAGTTG GATCAAGTTC TTCCTGCACC
    CCAGTTAGAG TCAGCCCTGT AACAGATATA CCTCCACCTA TAACTCAGTC TCCAGTCATC
    CTGCATCCTC TGACCTCCCC GCTGCAGGTG GGAGTGCCAC CCATGTCGCT GCCAATCATC
    CTCAACCCTG CATTAATTGA AGCTACCTCT CCAGTTCCGC TCTTGTCCAC TCCCCGACCT
    GCTGATGTAT CATCTGTCTC AGAGGTCAAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031761940.1
    CDS100..5772
    Translation

    Target ORF information:

    RefSeq Version XM_031906080.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031906080.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGA TCTCTCCATG TCTACTCTGC
    AAAATGGAGC TTAATAGTGG TGAACAGTCC AAAAACCTGA ACCAGTTAGA GAGGGTACTT
    CTTTTTAAGA GCCTGAAGTT GAAAATGAGA GCTGGAGGAA TGAGTGACTC CTCAAAATGG
    AAAAAGCAGA AGAAATCTCC TAGGCAGCCA CGTCATGTTA CCAAAGCTAT GGCGGCAGCA
    GAACCCTCCT CTCAGCAACC AGTAGTTAAT GGCACCACAA TTACTACTAA CCTCACTGGT
    GAAGTTCCTT TTATTGATGC TTCCTCTCCA GATGCTAATT TTCCCATTTC AGGGGTGAGT
    TCAGAGGCCA CGTCTGCTGT AGTCAGTCCT ACCACAGATA GTGGTGTAGA TATGTCCTCT
    CAGGCAACAT CTAAAGAAGA CCTTTCTGAA CTGGATCCTG CAAGTCCATC TCTTTCTGCC
    ATTCAGCAAT CAGGAGACAT TTGTCTTTCT GAACAGCAAA ACCAGGATGA TGATAAGATT
    GAAGACCTTA TGGAAAGGCA TACATATTCT GCTTCTGTGG AATCCATACC AGAAGTATTA
    GATGATAATG TGTCATTGGC TGATCAATCA GACTCCGCTT CAGTCCATGA TATGGATTAT
    GTAAATCCTA GAGGTGTGAG ATTTACCCAA TCTTCACAGA AAGAAGGAAC AGTCTTAGTA
    CCATATGGAC TGCCATGCAT TCGGGAATTA TTTCGTTTTC TTATTTCTTT AACCAACCCC
    CATGATCGAC ATAACTCCGA AGTGATGATA CACATGGGTT TACAGTTGAT CACAGTTGCT
    CTTGAGTCTG CACCTATTGA AAATTATGTT TCGCTTTTAG GCCTTGTAAA GGATGAACTT
    TGTCGACACT TATTTCAGCT ACTGAGCATT GAGAGACTGA ACCTATATGC AGCTTCCTTG
    CGAGCATGTT TTCTTTTGTT TGAGGGTATG AGGGAGCATT TGAAGTTCCA GCTAGAGATG
    TACATAAAGA AGCTCATGGA TATTATTACT GTGGAGAACC CTAAGATGCC TTATGAGATG
    AAAGAAATGG CTCTTGAAGC TATTGTGCAA TTGTGGAGAA TCCCGAGCTT TGTTACAGAA
    TTATACATTA ATTACGACTG TGATTATTAT TGCTCCAACC TTTTTGAAGA TCTCACTAAA
    CTGCTTTCCA AGAATGCCTT TCCAGTTTCT GGACAGCTGT ACACCACTCA TCTCCTTTCC
    CTGGAAGCTC TGCTAACTGT CATTGATAGC ACAGAATCAC ACTGCCAGGC TAAAAATCTA
    AGCAATGTGT CACAGGATAA GAAAGAAACT GGAAAACCAA GTGGAGATCT GTCAGAAGGG
    ACCAAAGATT CTGGAAATCT CAATGAACAC TCCATTGAAG TTAAGCCATT CACTGTTAGT
    GCAGAAAACA ATGGATCGAA GCCCCCCACA AGTGGCCACT TAATGGCAGA TAAAATGACT
    CTTGGGGTCC AAGAAACAGA GTACAGCACT GATGGAGGTG AGAAGAAGAC TTTAAAGAAG
    CCCCAACGCT TTTCCTGCTT ACTTCCGAAT GTTCAAGAGC TGAATGACAT TAAGACCAAA
    AAGAAGCTTC TTATAACTGG AACTGAGCAG TTCAACCAGA AACCAAAGAA AGGAGTGCAG
    TTTCTTCAGG AAAAAGACCT TTTGGCAACC CCCATGGATA ATGCTGAGGT AGCACAGTGG
    CTCAGAGAAA ATCCAAGGCT GGACAAGAAA ATGATTGGGG AGTTTGTGAG TGATCGCAAA
    AATCTGGACT TACTTGAAAG TTTTGTAGGA ACCTTCCATT TCCAAGGCCT GAGAGTTGAT
    GAAGCTTTAA GGCTTTACCT TGAAGCTTTT CGTTTGCCTG GCGAGGCACC TGTGATTCAG
    AGGTTACTGG AGGCTTTTAC AGAGCACTGG CGAAAATCCA ATGGAACTCC TTTTGCACAC
    AGTGATGCCT GCTTTGCTTT GGCTTATGCA GTTATCATGC TGAACACAGA CCAACACAAT
    CACAATGTCC GCAAGCAAAA TGTGCCAATG ACTTTAGAGG AATTCAGGAA AAATTTAAAG
    GGTGTTAATG GTGGAAAAGA TTTTGACCAA GATATGCTAG AGGATATTTA TCACGCAATC
    AAAAATGAAG AAATTGTAAT GCCTGAAGAG CAGACTGGCT TAGTAAAAGA GAATTATTTT
    TGGAATGTTT TGCTGCATCG GGGAGCCACT CCAGAAGGAA TGTTCTTGCA TGTGGATCCT
    GGAAGTTATG ACCATGACCT CTTTACTATG ACTTGGGGGC CAACAATAGC TGCCTTGTCC
    TATGTGTTTG ATAAGAGCAT GGATGAAACG ATTATTCAGA AAGCAATATC TGGTTTTAGG
    AAATGTGCAA TGATATCTGC TCACTATGGT CTCAGTGATG TTTTTGACAA TCTCATAATT
    TCCTTGTGCA AGTTTACAAC ACTTAGCAGT GAAGCCGTTG AAAATCTTCC CACTGTTTTT
    GGAAGCAACC TAAAAGCTCA GATCGCTGCA AAGACTGTGT TCCACTTATC CCACAGACAT
    GGTGACATCC TTCGAGAGGG ATGGAAAAAT ATTATGGACT CCATGCTGCA GCTTTTTAGA
    GCTGAGCTGC TTCCCAAGGC TATGGTTGAG GTTGAAGATT TTGTAGACCC CAATGGAAAA
    ATATCTCTTC AACGTGAGGA AATCCCTGCC AACCGTGGGG AATCCACTGT ACTAAGTTTT
    GTCAGCTGGC TGACTCTGAG TGGGACAGAA CAGTCAAGTC TAAGAGGACC TTCTACTGAA
    AATCAAGAGG CCAAGAAACT AGCGCTGGAA TGTATCAAGT TATGTGATCC AGAGAAACTG
    ATCACAGAGA GCAAGTTTCT TCAACTGGAG TCTTTGCAAG AACTAATGAA GGCTCTGATA
    TCTGTGACAC CAGATGAAGA AACGTATGAT GAAGAAGATG CTGCATTCTG CCTAGAAATG
    TTAATGAGAA TTGTGCTAGA AAACAGAGAT CGTGTTGGCT GTGTTTGGCA GGCTGTACGA
    GATCATCTCT ATCGTCTATG TGTCCATGCT GTAGAGTTCT GCTTCCTGGT TGAAAGAGCA
    GTTGTAGGGC TGCTGCGCTT GGCAATACGC CTTTTACGCA GAGAGGAAAT TAGTGGTCAG
    GTTATCCTCT CCTTGAGAAT TCTTTTAATG ATGAAACCAA CTGTGTTGTC TAAAGTGAGT
    CGCCAAGTTG CTTTTGGTGT GCATGAGCTC TTGAAAACCA ATGCTGCTAA CATCCATTCC
    AGCAATGACT GGTACACACT ATTCTGCCTT TTGGAGTGCA TTGGGGCAGG AGTGAAGCCG
    CCCCCTGCCT TACAGGTGAC GACGAGAGCT GACAATGACA CAGGTGCGCA GTCTGACAGT
    GAAGTTTCCT CATACCATCC AAGTGAAGTG AATCTTGATC GTGGATACAC CTCAGACTCT
    GAGGTATATG CAGAACACAG CAAGCAGTCA AAAATGCACC GCTCTGTGAC TGATGTGGAT
    ATGATGAACA GTGGCTGGCT GATGGTTGGA AAGGAAGATG TTGACTCTTC TAAAGTTGCT
    ACAGTTATTA ACAAACCTGT TGCTAATGCC CCAGTGAATC AATACAGCCT AACCATTGGT
    TTGGATTTTG GCCCTCACGA TACCAAATCT CTCATGAAGA GCGTGGAGTC CCTTTCTTTT
    ATTGTTCGAG ATGCTGCACA TGTCACACCA GAAAACTTTG AACTCTGTGT TAAAACAATC
    CGTGTCTTTG TAGAGGCAAG TCTAAATGGA GGTGTGTGGT ACAAATCTCA GGAGAAAAGG
    GTAAAGAACC ACAAATATGA CAGTAAATCC AACCGCTTCA AAAAGAAAAC CAAGGAAAAA
    GAAAATTCTG TCCGCCGTAC ACGGGCTTCA AATCAGCGAC AATACCGATC TCACAGTGAT
    GATGAGGAAG ATGAAAGTGT ACCAGCCAGC TATCACACTG TGTCATTACA GGTTAGTCAT
    GATCTTTTGG ACCTAATGCA CACACTTCAC ACAAGAGCTG CCACCATATA CAGTTCTTGG
    GCAGAGGAAC AGCGCAACCT AGAGACAGCT GCTGAGAAAA TTACAGCCGA TTCTAAGACA
    CTGTGGTCAA ACTGTTGGTG TCCTTTACTG CAAGGTATTG CTTGGCTCTG CTGTGATGCT
    CGGCGCCAGG TCAGAATGCA GGCTCTCACC TACCTGCAAA GGGCACTTTT AGTCCATGAC
    TTACAGGCTC TTGATGCACT TGAATGGGAA TCATGCTTTA ATAAGGTGCT ATTTCCATTG
    CTGACCAAAC TATTGGAAAA TATTAGTCCA GCTGATGTTG GCGGGATGGA GGAGACCAGA
    ATGAGAGGCT CCACTCTGCT CTCAAAGGTA TTTCTACAGC ATCTGTCTCC TCTTCTTTCT
    CTGTCGACAT TTGCTGCCTT GTGGCTTACT ATTCTTGATT TCATGGACAA GTACATGCAT
    GCTGGCTCCA GTGACTTACT GCCTGAGGCA ATTCCAGAGT CACTGAAAAA TATGCTTCTC
    GTAATGGACA CTGCCGGAAT ATTCCACAGT GCAGACTCGC GGACGGGCTA CTCTGATTTA
    TGGGAGATCA CGTGGGAAAG GATAGATTGC TTTTTACCTC GTCTAAGGGA AGAACTGTTT
    AAACAAACTG TGATACAAGA TCCACCCAAT ATAGTTACAA CAGAAACTCC TGTTGAGCCA
    AGACCTCCCC CTCAATCAAC TCAGTCAGAG AGACCAGCGG AGCAGGGACC TTGTGACTCA
    GAGACTCAAA GTTTAGCAAT CTCATCTGCT CCAGTAGTTG GATCAAGTTC TTCCTGCACC
    CCAGTTAGAG TCAGCCCTGT AACAGATATA CCTCCACCTA TAACTCAGTC TCCAGTCATC
    CTGCATCCTC TGACCTCCCC GCTGCAGGTG GGAGTGCCAC CCATGTCGCT GCCAATCATC
    CTCAACCCTG CATTAATTGA AGCTACCTCT CCAGTTCCGC TCTTGTCCAC TCCCCGACCT
    GCTGATGTAT CATCTGTCTC AGAGGTCAAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa62534
    Clone ID Related Accession (Same CDS sequence) XM_031906080.1 , XM_031906081.1 , XM_031906082.1
    Accession Version XM_031906081.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5673bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGA TCTCTCCATG TCTACTCTGC
    AAAATGGAGC TTAATAGTGG TGAACAGTCC AAAAACCTGA ACCAGTTAGA GAGGGTACTT
    CTTTTTAAGA GCCTGAAGTT GAAAATGAGA GCTGGAGGAA TGAGTGACTC CTCAAAATGG
    AAAAAGCAGA AGAAATCTCC TAGGCAGCCA CGTCATGTTA CCAAAGCTAT GGCGGCAGCA
    GAACCCTCCT CTCAGCAACC AGTAGTTAAT GGCACCACAA TTACTACTAA CCTCACTGGT
    GAAGTTCCTT TTATTGATGC TTCCTCTCCA GATGCTAATT TTCCCATTTC AGGGGTGAGT
    TCAGAGGCCA CGTCTGCTGT AGTCAGTCCT ACCACAGATA GTGGTGTAGA TATGTCCTCT
    CAGGCAACAT CTAAAGAAGA CCTTTCTGAA CTGGATCCTG CAAGTCCATC TCTTTCTGCC
    ATTCAGCAAT CAGGAGACAT TTGTCTTTCT GAACAGCAAA ACCAGGATGA TGATAAGATT
    GAAGACCTTA TGGAAAGGCA TACATATTCT GCTTCTGTGG AATCCATACC AGAAGTATTA
    GATGATAATG TGTCATTGGC TGATCAATCA GACTCCGCTT CAGTCCATGA TATGGATTAT
    GTAAATCCTA GAGGTGTGAG ATTTACCCAA TCTTCACAGA AAGAAGGAAC AGTCTTAGTA
    CCATATGGAC TGCCATGCAT TCGGGAATTA TTTCGTTTTC TTATTTCTTT AACCAACCCC
    CATGATCGAC ATAACTCCGA AGTGATGATA CACATGGGTT TACAGTTGAT CACAGTTGCT
    CTTGAGTCTG CACCTATTGA AAATTATGTT TCGCTTTTAG GCCTTGTAAA GGATGAACTT
    TGTCGACACT TATTTCAGCT ACTGAGCATT GAGAGACTGA ACCTATATGC AGCTTCCTTG
    CGAGCATGTT TTCTTTTGTT TGAGGGTATG AGGGAGCATT TGAAGTTCCA GCTAGAGATG
    TACATAAAGA AGCTCATGGA TATTATTACT GTGGAGAACC CTAAGATGCC TTATGAGATG
    AAAGAAATGG CTCTTGAAGC TATTGTGCAA TTGTGGAGAA TCCCGAGCTT TGTTACAGAA
    TTATACATTA ATTACGACTG TGATTATTAT TGCTCCAACC TTTTTGAAGA TCTCACTAAA
    CTGCTTTCCA AGAATGCCTT TCCAGTTTCT GGACAGCTGT ACACCACTCA TCTCCTTTCC
    CTGGAAGCTC TGCTAACTGT CATTGATAGC ACAGAATCAC ACTGCCAGGC TAAAAATCTA
    AGCAATGTGT CACAGGATAA GAAAGAAACT GGAAAACCAA GTGGAGATCT GTCAGAAGGG
    ACCAAAGATT CTGGAAATCT CAATGAACAC TCCATTGAAG TTAAGCCATT CACTGTTAGT
    GCAGAAAACA ATGGATCGAA GCCCCCCACA AGTGGCCACT TAATGGCAGA TAAAATGACT
    CTTGGGGTCC AAGAAACAGA GTACAGCACT GATGGAGGTG AGAAGAAGAC TTTAAAGAAG
    CCCCAACGCT TTTCCTGCTT ACTTCCGAAT GTTCAAGAGC TGAATGACAT TAAGACCAAA
    AAGAAGCTTC TTATAACTGG AACTGAGCAG TTCAACCAGA AACCAAAGAA AGGAGTGCAG
    TTTCTTCAGG AAAAAGACCT TTTGGCAACC CCCATGGATA ATGCTGAGGT AGCACAGTGG
    CTCAGAGAAA ATCCAAGGCT GGACAAGAAA ATGATTGGGG AGTTTGTGAG TGATCGCAAA
    AATCTGGACT TACTTGAAAG TTTTGTAGGA ACCTTCCATT TCCAAGGCCT GAGAGTTGAT
    GAAGCTTTAA GGCTTTACCT TGAAGCTTTT CGTTTGCCTG GCGAGGCACC TGTGATTCAG
    AGGTTACTGG AGGCTTTTAC AGAGCACTGG CGAAAATCCA ATGGAACTCC TTTTGCACAC
    AGTGATGCCT GCTTTGCTTT GGCTTATGCA GTTATCATGC TGAACACAGA CCAACACAAT
    CACAATGTCC GCAAGCAAAA TGTGCCAATG ACTTTAGAGG AATTCAGGAA AAATTTAAAG
    GGTGTTAATG GTGGAAAAGA TTTTGACCAA GATATGCTAG AGGATATTTA TCACGCAATC
    AAAAATGAAG AAATTGTAAT GCCTGAAGAG CAGACTGGCT TAGTAAAAGA GAATTATTTT
    TGGAATGTTT TGCTGCATCG GGGAGCCACT CCAGAAGGAA TGTTCTTGCA TGTGGATCCT
    GGAAGTTATG ACCATGACCT CTTTACTATG ACTTGGGGGC CAACAATAGC TGCCTTGTCC
    TATGTGTTTG ATAAGAGCAT GGATGAAACG ATTATTCAGA AAGCAATATC TGGTTTTAGG
    AAATGTGCAA TGATATCTGC TCACTATGGT CTCAGTGATG TTTTTGACAA TCTCATAATT
    TCCTTGTGCA AGTTTACAAC ACTTAGCAGT GAAGCCGTTG AAAATCTTCC CACTGTTTTT
    GGAAGCAACC TAAAAGCTCA GATCGCTGCA AAGACTGTGT TCCACTTATC CCACAGACAT
    GGTGACATCC TTCGAGAGGG ATGGAAAAAT ATTATGGACT CCATGCTGCA GCTTTTTAGA
    GCTGAGCTGC TTCCCAAGGC TATGGTTGAG GTTGAAGATT TTGTAGACCC CAATGGAAAA
    ATATCTCTTC AACGTGAGGA AATCCCTGCC AACCGTGGGG AATCCACTGT ACTAAGTTTT
    GTCAGCTGGC TGACTCTGAG TGGGACAGAA CAGTCAAGTC TAAGAGGACC TTCTACTGAA
    AATCAAGAGG CCAAGAAACT AGCGCTGGAA TGTATCAAGT TATGTGATCC AGAGAAACTG
    ATCACAGAGA GCAAGTTTCT TCAACTGGAG TCTTTGCAAG AACTAATGAA GGCTCTGATA
    TCTGTGACAC CAGATGAAGA AACGTATGAT GAAGAAGATG CTGCATTCTG CCTAGAAATG
    TTAATGAGAA TTGTGCTAGA AAACAGAGAT CGTGTTGGCT GTGTTTGGCA GGCTGTACGA
    GATCATCTCT ATCGTCTATG TGTCCATGCT GTAGAGTTCT GCTTCCTGGT TGAAAGAGCA
    GTTGTAGGGC TGCTGCGCTT GGCAATACGC CTTTTACGCA GAGAGGAAAT TAGTGGTCAG
    GTTATCCTCT CCTTGAGAAT TCTTTTAATG ATGAAACCAA CTGTGTTGTC TAAAGTGAGT
    CGCCAAGTTG CTTTTGGTGT GCATGAGCTC TTGAAAACCA ATGCTGCTAA CATCCATTCC
    AGCAATGACT GGTACACACT ATTCTGCCTT TTGGAGTGCA TTGGGGCAGG AGTGAAGCCG
    CCCCCTGCCT TACAGGTGAC GACGAGAGCT GACAATGACA CAGGTGCGCA GTCTGACAGT
    GAAGTTTCCT CATACCATCC AAGTGAAGTG AATCTTGATC GTGGATACAC CTCAGACTCT
    GAGGTATATG CAGAACACAG CAAGCAGTCA AAAATGCACC GCTCTGTGAC TGATGTGGAT
    ATGATGAACA GTGGCTGGCT GATGGTTGGA AAGGAAGATG TTGACTCTTC TAAAGTTGCT
    ACAGTTATTA ACAAACCTGT TGCTAATGCC CCAGTGAATC AATACAGCCT AACCATTGGT
    TTGGATTTTG GCCCTCACGA TACCAAATCT CTCATGAAGA GCGTGGAGTC CCTTTCTTTT
    ATTGTTCGAG ATGCTGCACA TGTCACACCA GAAAACTTTG AACTCTGTGT TAAAACAATC
    CGTGTCTTTG TAGAGGCAAG TCTAAATGGA GGTGTGTGGT ACAAATCTCA GGAGAAAAGG
    GTAAAGAACC ACAAATATGA CAGTAAATCC AACCGCTTCA AAAAGAAAAC CAAGGAAAAA
    GAAAATTCTG TCCGCCGTAC ACGGGCTTCA AATCAGCGAC AATACCGATC TCACAGTGAT
    GATGAGGAAG ATGAAAGTGT ACCAGCCAGC TATCACACTG TGTCATTACA GGTTAGTCAT
    GATCTTTTGG ACCTAATGCA CACACTTCAC ACAAGAGCTG CCACCATATA CAGTTCTTGG
    GCAGAGGAAC AGCGCAACCT AGAGACAGCT GCTGAGAAAA TTACAGCCGA TTCTAAGACA
    CTGTGGTCAA ACTGTTGGTG TCCTTTACTG CAAGGTATTG CTTGGCTCTG CTGTGATGCT
    CGGCGCCAGG TCAGAATGCA GGCTCTCACC TACCTGCAAA GGGCACTTTT AGTCCATGAC
    TTACAGGCTC TTGATGCACT TGAATGGGAA TCATGCTTTA ATAAGGTGCT ATTTCCATTG
    CTGACCAAAC TATTGGAAAA TATTAGTCCA GCTGATGTTG GCGGGATGGA GGAGACCAGA
    ATGAGAGGCT CCACTCTGCT CTCAAAGGTA TTTCTACAGC ATCTGTCTCC TCTTCTTTCT
    CTGTCGACAT TTGCTGCCTT GTGGCTTACT ATTCTTGATT TCATGGACAA GTACATGCAT
    GCTGGCTCCA GTGACTTACT GCCTGAGGCA ATTCCAGAGT CACTGAAAAA TATGCTTCTC
    GTAATGGACA CTGCCGGAAT ATTCCACAGT GCAGACTCGC GGACGGGCTA CTCTGATTTA
    TGGGAGATCA CGTGGGAAAG GATAGATTGC TTTTTACCTC GTCTAAGGGA AGAACTGTTT
    AAACAAACTG TGATACAAGA TCCACCCAAT ATAGTTACAA CAGAAACTCC TGTTGAGCCA
    AGACCTCCCC CTCAATCAAC TCAGTCAGAG AGACCAGCGG AGCAGGGACC TTGTGACTCA
    GAGACTCAAA GTTTAGCAAT CTCATCTGCT CCAGTAGTTG GATCAAGTTC TTCCTGCACC
    CCAGTTAGAG TCAGCCCTGT AACAGATATA CCTCCACCTA TAACTCAGTC TCCAGTCATC
    CTGCATCCTC TGACCTCCCC GCTGCAGGTG GGAGTGCCAC CCATGTCGCT GCCAATCATC
    CTCAACCCTG CATTAATTGA AGCTACCTCT CCAGTTCCGC TCTTGTCCAC TCCCCGACCT
    GCTGATGTAT CATCTGTCTC AGAGGTCAAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031761941.1
    CDS263..5935
    Translation

    Target ORF information:

    RefSeq Version XM_031906081.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031906081.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGA TCTCTCCATG TCTACTCTGC
    AAAATGGAGC TTAATAGTGG TGAACAGTCC AAAAACCTGA ACCAGTTAGA GAGGGTACTT
    CTTTTTAAGA GCCTGAAGTT GAAAATGAGA GCTGGAGGAA TGAGTGACTC CTCAAAATGG
    AAAAAGCAGA AGAAATCTCC TAGGCAGCCA CGTCATGTTA CCAAAGCTAT GGCGGCAGCA
    GAACCCTCCT CTCAGCAACC AGTAGTTAAT GGCACCACAA TTACTACTAA CCTCACTGGT
    GAAGTTCCTT TTATTGATGC TTCCTCTCCA GATGCTAATT TTCCCATTTC AGGGGTGAGT
    TCAGAGGCCA CGTCTGCTGT AGTCAGTCCT ACCACAGATA GTGGTGTAGA TATGTCCTCT
    CAGGCAACAT CTAAAGAAGA CCTTTCTGAA CTGGATCCTG CAAGTCCATC TCTTTCTGCC
    ATTCAGCAAT CAGGAGACAT TTGTCTTTCT GAACAGCAAA ACCAGGATGA TGATAAGATT
    GAAGACCTTA TGGAAAGGCA TACATATTCT GCTTCTGTGG AATCCATACC AGAAGTATTA
    GATGATAATG TGTCATTGGC TGATCAATCA GACTCCGCTT CAGTCCATGA TATGGATTAT
    GTAAATCCTA GAGGTGTGAG ATTTACCCAA TCTTCACAGA AAGAAGGAAC AGTCTTAGTA
    CCATATGGAC TGCCATGCAT TCGGGAATTA TTTCGTTTTC TTATTTCTTT AACCAACCCC
    CATGATCGAC ATAACTCCGA AGTGATGATA CACATGGGTT TACAGTTGAT CACAGTTGCT
    CTTGAGTCTG CACCTATTGA AAATTATGTT TCGCTTTTAG GCCTTGTAAA GGATGAACTT
    TGTCGACACT TATTTCAGCT ACTGAGCATT GAGAGACTGA ACCTATATGC AGCTTCCTTG
    CGAGCATGTT TTCTTTTGTT TGAGGGTATG AGGGAGCATT TGAAGTTCCA GCTAGAGATG
    TACATAAAGA AGCTCATGGA TATTATTACT GTGGAGAACC CTAAGATGCC TTATGAGATG
    AAAGAAATGG CTCTTGAAGC TATTGTGCAA TTGTGGAGAA TCCCGAGCTT TGTTACAGAA
    TTATACATTA ATTACGACTG TGATTATTAT TGCTCCAACC TTTTTGAAGA TCTCACTAAA
    CTGCTTTCCA AGAATGCCTT TCCAGTTTCT GGACAGCTGT ACACCACTCA TCTCCTTTCC
    CTGGAAGCTC TGCTAACTGT CATTGATAGC ACAGAATCAC ACTGCCAGGC TAAAAATCTA
    AGCAATGTGT CACAGGATAA GAAAGAAACT GGAAAACCAA GTGGAGATCT GTCAGAAGGG
    ACCAAAGATT CTGGAAATCT CAATGAACAC TCCATTGAAG TTAAGCCATT CACTGTTAGT
    GCAGAAAACA ATGGATCGAA GCCCCCCACA AGTGGCCACT TAATGGCAGA TAAAATGACT
    CTTGGGGTCC AAGAAACAGA GTACAGCACT GATGGAGGTG AGAAGAAGAC TTTAAAGAAG
    CCCCAACGCT TTTCCTGCTT ACTTCCGAAT GTTCAAGAGC TGAATGACAT TAAGACCAAA
    AAGAAGCTTC TTATAACTGG AACTGAGCAG TTCAACCAGA AACCAAAGAA AGGAGTGCAG
    TTTCTTCAGG AAAAAGACCT TTTGGCAACC CCCATGGATA ATGCTGAGGT AGCACAGTGG
    CTCAGAGAAA ATCCAAGGCT GGACAAGAAA ATGATTGGGG AGTTTGTGAG TGATCGCAAA
    AATCTGGACT TACTTGAAAG TTTTGTAGGA ACCTTCCATT TCCAAGGCCT GAGAGTTGAT
    GAAGCTTTAA GGCTTTACCT TGAAGCTTTT CGTTTGCCTG GCGAGGCACC TGTGATTCAG
    AGGTTACTGG AGGCTTTTAC AGAGCACTGG CGAAAATCCA ATGGAACTCC TTTTGCACAC
    AGTGATGCCT GCTTTGCTTT GGCTTATGCA GTTATCATGC TGAACACAGA CCAACACAAT
    CACAATGTCC GCAAGCAAAA TGTGCCAATG ACTTTAGAGG AATTCAGGAA AAATTTAAAG
    GGTGTTAATG GTGGAAAAGA TTTTGACCAA GATATGCTAG AGGATATTTA TCACGCAATC
    AAAAATGAAG AAATTGTAAT GCCTGAAGAG CAGACTGGCT TAGTAAAAGA GAATTATTTT
    TGGAATGTTT TGCTGCATCG GGGAGCCACT CCAGAAGGAA TGTTCTTGCA TGTGGATCCT
    GGAAGTTATG ACCATGACCT CTTTACTATG ACTTGGGGGC CAACAATAGC TGCCTTGTCC
    TATGTGTTTG ATAAGAGCAT GGATGAAACG ATTATTCAGA AAGCAATATC TGGTTTTAGG
    AAATGTGCAA TGATATCTGC TCACTATGGT CTCAGTGATG TTTTTGACAA TCTCATAATT
    TCCTTGTGCA AGTTTACAAC ACTTAGCAGT GAAGCCGTTG AAAATCTTCC CACTGTTTTT
    GGAAGCAACC TAAAAGCTCA GATCGCTGCA AAGACTGTGT TCCACTTATC CCACAGACAT
    GGTGACATCC TTCGAGAGGG ATGGAAAAAT ATTATGGACT CCATGCTGCA GCTTTTTAGA
    GCTGAGCTGC TTCCCAAGGC TATGGTTGAG GTTGAAGATT TTGTAGACCC CAATGGAAAA
    ATATCTCTTC AACGTGAGGA AATCCCTGCC AACCGTGGGG AATCCACTGT ACTAAGTTTT
    GTCAGCTGGC TGACTCTGAG TGGGACAGAA CAGTCAAGTC TAAGAGGACC TTCTACTGAA
    AATCAAGAGG CCAAGAAACT AGCGCTGGAA TGTATCAAGT TATGTGATCC AGAGAAACTG
    ATCACAGAGA GCAAGTTTCT TCAACTGGAG TCTTTGCAAG AACTAATGAA GGCTCTGATA
    TCTGTGACAC CAGATGAAGA AACGTATGAT GAAGAAGATG CTGCATTCTG CCTAGAAATG
    TTAATGAGAA TTGTGCTAGA AAACAGAGAT CGTGTTGGCT GTGTTTGGCA GGCTGTACGA
    GATCATCTCT ATCGTCTATG TGTCCATGCT GTAGAGTTCT GCTTCCTGGT TGAAAGAGCA
    GTTGTAGGGC TGCTGCGCTT GGCAATACGC CTTTTACGCA GAGAGGAAAT TAGTGGTCAG
    GTTATCCTCT CCTTGAGAAT TCTTTTAATG ATGAAACCAA CTGTGTTGTC TAAAGTGAGT
    CGCCAAGTTG CTTTTGGTGT GCATGAGCTC TTGAAAACCA ATGCTGCTAA CATCCATTCC
    AGCAATGACT GGTACACACT ATTCTGCCTT TTGGAGTGCA TTGGGGCAGG AGTGAAGCCG
    CCCCCTGCCT TACAGGTGAC GACGAGAGCT GACAATGACA CAGGTGCGCA GTCTGACAGT
    GAAGTTTCCT CATACCATCC AAGTGAAGTG AATCTTGATC GTGGATACAC CTCAGACTCT
    GAGGTATATG CAGAACACAG CAAGCAGTCA AAAATGCACC GCTCTGTGAC TGATGTGGAT
    ATGATGAACA GTGGCTGGCT GATGGTTGGA AAGGAAGATG TTGACTCTTC TAAAGTTGCT
    ACAGTTATTA ACAAACCTGT TGCTAATGCC CCAGTGAATC AATACAGCCT AACCATTGGT
    TTGGATTTTG GCCCTCACGA TACCAAATCT CTCATGAAGA GCGTGGAGTC CCTTTCTTTT
    ATTGTTCGAG ATGCTGCACA TGTCACACCA GAAAACTTTG AACTCTGTGT TAAAACAATC
    CGTGTCTTTG TAGAGGCAAG TCTAAATGGA GGTGTGTGGT ACAAATCTCA GGAGAAAAGG
    GTAAAGAACC ACAAATATGA CAGTAAATCC AACCGCTTCA AAAAGAAAAC CAAGGAAAAA
    GAAAATTCTG TCCGCCGTAC ACGGGCTTCA AATCAGCGAC AATACCGATC TCACAGTGAT
    GATGAGGAAG ATGAAAGTGT ACCAGCCAGC TATCACACTG TGTCATTACA GGTTAGTCAT
    GATCTTTTGG ACCTAATGCA CACACTTCAC ACAAGAGCTG CCACCATATA CAGTTCTTGG
    GCAGAGGAAC AGCGCAACCT AGAGACAGCT GCTGAGAAAA TTACAGCCGA TTCTAAGACA
    CTGTGGTCAA ACTGTTGGTG TCCTTTACTG CAAGGTATTG CTTGGCTCTG CTGTGATGCT
    CGGCGCCAGG TCAGAATGCA GGCTCTCACC TACCTGCAAA GGGCACTTTT AGTCCATGAC
    TTACAGGCTC TTGATGCACT TGAATGGGAA TCATGCTTTA ATAAGGTGCT ATTTCCATTG
    CTGACCAAAC TATTGGAAAA TATTAGTCCA GCTGATGTTG GCGGGATGGA GGAGACCAGA
    ATGAGAGGCT CCACTCTGCT CTCAAAGGTA TTTCTACAGC ATCTGTCTCC TCTTCTTTCT
    CTGTCGACAT TTGCTGCCTT GTGGCTTACT ATTCTTGATT TCATGGACAA GTACATGCAT
    GCTGGCTCCA GTGACTTACT GCCTGAGGCA ATTCCAGAGT CACTGAAAAA TATGCTTCTC
    GTAATGGACA CTGCCGGAAT ATTCCACAGT GCAGACTCGC GGACGGGCTA CTCTGATTTA
    TGGGAGATCA CGTGGGAAAG GATAGATTGC TTTTTACCTC GTCTAAGGGA AGAACTGTTT
    AAACAAACTG TGATACAAGA TCCACCCAAT ATAGTTACAA CAGAAACTCC TGTTGAGCCA
    AGACCTCCCC CTCAATCAAC TCAGTCAGAG AGACCAGCGG AGCAGGGACC TTGTGACTCA
    GAGACTCAAA GTTTAGCAAT CTCATCTGCT CCAGTAGTTG GATCAAGTTC TTCCTGCACC
    CCAGTTAGAG TCAGCCCTGT AACAGATATA CCTCCACCTA TAACTCAGTC TCCAGTCATC
    CTGCATCCTC TGACCTCCCC GCTGCAGGTG GGAGTGCCAC CCATGTCGCT GCCAATCATC
    CTCAACCCTG CATTAATTGA AGCTACCTCT CCAGTTCCGC TCTTGTCCAC TCCCCGACCT
    GCTGATGTAT CATCTGTCTC AGAGGTCAAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa62534
    Clone ID Related Accession (Same CDS sequence) XM_031906080.1 , XM_031906081.1 , XM_031906082.1
    Accession Version XM_031906082.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5673bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGA TCTCTCCATG TCTACTCTGC
    AAAATGGAGC TTAATAGTGG TGAACAGTCC AAAAACCTGA ACCAGTTAGA GAGGGTACTT
    CTTTTTAAGA GCCTGAAGTT GAAAATGAGA GCTGGAGGAA TGAGTGACTC CTCAAAATGG
    AAAAAGCAGA AGAAATCTCC TAGGCAGCCA CGTCATGTTA CCAAAGCTAT GGCGGCAGCA
    GAACCCTCCT CTCAGCAACC AGTAGTTAAT GGCACCACAA TTACTACTAA CCTCACTGGT
    GAAGTTCCTT TTATTGATGC TTCCTCTCCA GATGCTAATT TTCCCATTTC AGGGGTGAGT
    TCAGAGGCCA CGTCTGCTGT AGTCAGTCCT ACCACAGATA GTGGTGTAGA TATGTCCTCT
    CAGGCAACAT CTAAAGAAGA CCTTTCTGAA CTGGATCCTG CAAGTCCATC TCTTTCTGCC
    ATTCAGCAAT CAGGAGACAT TTGTCTTTCT GAACAGCAAA ACCAGGATGA TGATAAGATT
    GAAGACCTTA TGGAAAGGCA TACATATTCT GCTTCTGTGG AATCCATACC AGAAGTATTA
    GATGATAATG TGTCATTGGC TGATCAATCA GACTCCGCTT CAGTCCATGA TATGGATTAT
    GTAAATCCTA GAGGTGTGAG ATTTACCCAA TCTTCACAGA AAGAAGGAAC AGTCTTAGTA
    CCATATGGAC TGCCATGCAT TCGGGAATTA TTTCGTTTTC TTATTTCTTT AACCAACCCC
    CATGATCGAC ATAACTCCGA AGTGATGATA CACATGGGTT TACAGTTGAT CACAGTTGCT
    CTTGAGTCTG CACCTATTGA AAATTATGTT TCGCTTTTAG GCCTTGTAAA GGATGAACTT
    TGTCGACACT TATTTCAGCT ACTGAGCATT GAGAGACTGA ACCTATATGC AGCTTCCTTG
    CGAGCATGTT TTCTTTTGTT TGAGGGTATG AGGGAGCATT TGAAGTTCCA GCTAGAGATG
    TACATAAAGA AGCTCATGGA TATTATTACT GTGGAGAACC CTAAGATGCC TTATGAGATG
    AAAGAAATGG CTCTTGAAGC TATTGTGCAA TTGTGGAGAA TCCCGAGCTT TGTTACAGAA
    TTATACATTA ATTACGACTG TGATTATTAT TGCTCCAACC TTTTTGAAGA TCTCACTAAA
    CTGCTTTCCA AGAATGCCTT TCCAGTTTCT GGACAGCTGT ACACCACTCA TCTCCTTTCC
    CTGGAAGCTC TGCTAACTGT CATTGATAGC ACAGAATCAC ACTGCCAGGC TAAAAATCTA
    AGCAATGTGT CACAGGATAA GAAAGAAACT GGAAAACCAA GTGGAGATCT GTCAGAAGGG
    ACCAAAGATT CTGGAAATCT CAATGAACAC TCCATTGAAG TTAAGCCATT CACTGTTAGT
    GCAGAAAACA ATGGATCGAA GCCCCCCACA AGTGGCCACT TAATGGCAGA TAAAATGACT
    CTTGGGGTCC AAGAAACAGA GTACAGCACT GATGGAGGTG AGAAGAAGAC TTTAAAGAAG
    CCCCAACGCT TTTCCTGCTT ACTTCCGAAT GTTCAAGAGC TGAATGACAT TAAGACCAAA
    AAGAAGCTTC TTATAACTGG AACTGAGCAG TTCAACCAGA AACCAAAGAA AGGAGTGCAG
    TTTCTTCAGG AAAAAGACCT TTTGGCAACC CCCATGGATA ATGCTGAGGT AGCACAGTGG
    CTCAGAGAAA ATCCAAGGCT GGACAAGAAA ATGATTGGGG AGTTTGTGAG TGATCGCAAA
    AATCTGGACT TACTTGAAAG TTTTGTAGGA ACCTTCCATT TCCAAGGCCT GAGAGTTGAT
    GAAGCTTTAA GGCTTTACCT TGAAGCTTTT CGTTTGCCTG GCGAGGCACC TGTGATTCAG
    AGGTTACTGG AGGCTTTTAC AGAGCACTGG CGAAAATCCA ATGGAACTCC TTTTGCACAC
    AGTGATGCCT GCTTTGCTTT GGCTTATGCA GTTATCATGC TGAACACAGA CCAACACAAT
    CACAATGTCC GCAAGCAAAA TGTGCCAATG ACTTTAGAGG AATTCAGGAA AAATTTAAAG
    GGTGTTAATG GTGGAAAAGA TTTTGACCAA GATATGCTAG AGGATATTTA TCACGCAATC
    AAAAATGAAG AAATTGTAAT GCCTGAAGAG CAGACTGGCT TAGTAAAAGA GAATTATTTT
    TGGAATGTTT TGCTGCATCG GGGAGCCACT CCAGAAGGAA TGTTCTTGCA TGTGGATCCT
    GGAAGTTATG ACCATGACCT CTTTACTATG ACTTGGGGGC CAACAATAGC TGCCTTGTCC
    TATGTGTTTG ATAAGAGCAT GGATGAAACG ATTATTCAGA AAGCAATATC TGGTTTTAGG
    AAATGTGCAA TGATATCTGC TCACTATGGT CTCAGTGATG TTTTTGACAA TCTCATAATT
    TCCTTGTGCA AGTTTACAAC ACTTAGCAGT GAAGCCGTTG AAAATCTTCC CACTGTTTTT
    GGAAGCAACC TAAAAGCTCA GATCGCTGCA AAGACTGTGT TCCACTTATC CCACAGACAT
    GGTGACATCC TTCGAGAGGG ATGGAAAAAT ATTATGGACT CCATGCTGCA GCTTTTTAGA
    GCTGAGCTGC TTCCCAAGGC TATGGTTGAG GTTGAAGATT TTGTAGACCC CAATGGAAAA
    ATATCTCTTC AACGTGAGGA AATCCCTGCC AACCGTGGGG AATCCACTGT ACTAAGTTTT
    GTCAGCTGGC TGACTCTGAG TGGGACAGAA CAGTCAAGTC TAAGAGGACC TTCTACTGAA
    AATCAAGAGG CCAAGAAACT AGCGCTGGAA TGTATCAAGT TATGTGATCC AGAGAAACTG
    ATCACAGAGA GCAAGTTTCT TCAACTGGAG TCTTTGCAAG AACTAATGAA GGCTCTGATA
    TCTGTGACAC CAGATGAAGA AACGTATGAT GAAGAAGATG CTGCATTCTG CCTAGAAATG
    TTAATGAGAA TTGTGCTAGA AAACAGAGAT CGTGTTGGCT GTGTTTGGCA GGCTGTACGA
    GATCATCTCT ATCGTCTATG TGTCCATGCT GTAGAGTTCT GCTTCCTGGT TGAAAGAGCA
    GTTGTAGGGC TGCTGCGCTT GGCAATACGC CTTTTACGCA GAGAGGAAAT TAGTGGTCAG
    GTTATCCTCT CCTTGAGAAT TCTTTTAATG ATGAAACCAA CTGTGTTGTC TAAAGTGAGT
    CGCCAAGTTG CTTTTGGTGT GCATGAGCTC TTGAAAACCA ATGCTGCTAA CATCCATTCC
    AGCAATGACT GGTACACACT ATTCTGCCTT TTGGAGTGCA TTGGGGCAGG AGTGAAGCCG
    CCCCCTGCCT TACAGGTGAC GACGAGAGCT GACAATGACA CAGGTGCGCA GTCTGACAGT
    GAAGTTTCCT CATACCATCC AAGTGAAGTG AATCTTGATC GTGGATACAC CTCAGACTCT
    GAGGTATATG CAGAACACAG CAAGCAGTCA AAAATGCACC GCTCTGTGAC TGATGTGGAT
    ATGATGAACA GTGGCTGGCT GATGGTTGGA AAGGAAGATG TTGACTCTTC TAAAGTTGCT
    ACAGTTATTA ACAAACCTGT TGCTAATGCC CCAGTGAATC AATACAGCCT AACCATTGGT
    TTGGATTTTG GCCCTCACGA TACCAAATCT CTCATGAAGA GCGTGGAGTC CCTTTCTTTT
    ATTGTTCGAG ATGCTGCACA TGTCACACCA GAAAACTTTG AACTCTGTGT TAAAACAATC
    CGTGTCTTTG TAGAGGCAAG TCTAAATGGA GGTGTGTGGT ACAAATCTCA GGAGAAAAGG
    GTAAAGAACC ACAAATATGA CAGTAAATCC AACCGCTTCA AAAAGAAAAC CAAGGAAAAA
    GAAAATTCTG TCCGCCGTAC ACGGGCTTCA AATCAGCGAC AATACCGATC TCACAGTGAT
    GATGAGGAAG ATGAAAGTGT ACCAGCCAGC TATCACACTG TGTCATTACA GGTTAGTCAT
    GATCTTTTGG ACCTAATGCA CACACTTCAC ACAAGAGCTG CCACCATATA CAGTTCTTGG
    GCAGAGGAAC AGCGCAACCT AGAGACAGCT GCTGAGAAAA TTACAGCCGA TTCTAAGACA
    CTGTGGTCAA ACTGTTGGTG TCCTTTACTG CAAGGTATTG CTTGGCTCTG CTGTGATGCT
    CGGCGCCAGG TCAGAATGCA GGCTCTCACC TACCTGCAAA GGGCACTTTT AGTCCATGAC
    TTACAGGCTC TTGATGCACT TGAATGGGAA TCATGCTTTA ATAAGGTGCT ATTTCCATTG
    CTGACCAAAC TATTGGAAAA TATTAGTCCA GCTGATGTTG GCGGGATGGA GGAGACCAGA
    ATGAGAGGCT CCACTCTGCT CTCAAAGGTA TTTCTACAGC ATCTGTCTCC TCTTCTTTCT
    CTGTCGACAT TTGCTGCCTT GTGGCTTACT ATTCTTGATT TCATGGACAA GTACATGCAT
    GCTGGCTCCA GTGACTTACT GCCTGAGGCA ATTCCAGAGT CACTGAAAAA TATGCTTCTC
    GTAATGGACA CTGCCGGAAT ATTCCACAGT GCAGACTCGC GGACGGGCTA CTCTGATTTA
    TGGGAGATCA CGTGGGAAAG GATAGATTGC TTTTTACCTC GTCTAAGGGA AGAACTGTTT
    AAACAAACTG TGATACAAGA TCCACCCAAT ATAGTTACAA CAGAAACTCC TGTTGAGCCA
    AGACCTCCCC CTCAATCAAC TCAGTCAGAG AGACCAGCGG AGCAGGGACC TTGTGACTCA
    GAGACTCAAA GTTTAGCAAT CTCATCTGCT CCAGTAGTTG GATCAAGTTC TTCCTGCACC
    CCAGTTAGAG TCAGCCCTGT AACAGATATA CCTCCACCTA TAACTCAGTC TCCAGTCATC
    CTGCATCCTC TGACCTCCCC GCTGCAGGTG GGAGTGCCAC CCATGTCGCT GCCAATCATC
    CTCAACCCTG CATTAATTGA AGCTACCTCT CCAGTTCCGC TCTTGTCCAC TCCCCGACCT
    GCTGATGTAT CATCTGTCTC AGAGGTCAAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031761942.1
    CDS86..5758
    Translation

    Target ORF information:

    RefSeq Version XM_031906082.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031906082.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGA TCTCTCCATG TCTACTCTGC
    AAAATGGAGC TTAATAGTGG TGAACAGTCC AAAAACCTGA ACCAGTTAGA GAGGGTACTT
    CTTTTTAAGA GCCTGAAGTT GAAAATGAGA GCTGGAGGAA TGAGTGACTC CTCAAAATGG
    AAAAAGCAGA AGAAATCTCC TAGGCAGCCA CGTCATGTTA CCAAAGCTAT GGCGGCAGCA
    GAACCCTCCT CTCAGCAACC AGTAGTTAAT GGCACCACAA TTACTACTAA CCTCACTGGT
    GAAGTTCCTT TTATTGATGC TTCCTCTCCA GATGCTAATT TTCCCATTTC AGGGGTGAGT
    TCAGAGGCCA CGTCTGCTGT AGTCAGTCCT ACCACAGATA GTGGTGTAGA TATGTCCTCT
    CAGGCAACAT CTAAAGAAGA CCTTTCTGAA CTGGATCCTG CAAGTCCATC TCTTTCTGCC
    ATTCAGCAAT CAGGAGACAT TTGTCTTTCT GAACAGCAAA ACCAGGATGA TGATAAGATT
    GAAGACCTTA TGGAAAGGCA TACATATTCT GCTTCTGTGG AATCCATACC AGAAGTATTA
    GATGATAATG TGTCATTGGC TGATCAATCA GACTCCGCTT CAGTCCATGA TATGGATTAT
    GTAAATCCTA GAGGTGTGAG ATTTACCCAA TCTTCACAGA AAGAAGGAAC AGTCTTAGTA
    CCATATGGAC TGCCATGCAT TCGGGAATTA TTTCGTTTTC TTATTTCTTT AACCAACCCC
    CATGATCGAC ATAACTCCGA AGTGATGATA CACATGGGTT TACAGTTGAT CACAGTTGCT
    CTTGAGTCTG CACCTATTGA AAATTATGTT TCGCTTTTAG GCCTTGTAAA GGATGAACTT
    TGTCGACACT TATTTCAGCT ACTGAGCATT GAGAGACTGA ACCTATATGC AGCTTCCTTG
    CGAGCATGTT TTCTTTTGTT TGAGGGTATG AGGGAGCATT TGAAGTTCCA GCTAGAGATG
    TACATAAAGA AGCTCATGGA TATTATTACT GTGGAGAACC CTAAGATGCC TTATGAGATG
    AAAGAAATGG CTCTTGAAGC TATTGTGCAA TTGTGGAGAA TCCCGAGCTT TGTTACAGAA
    TTATACATTA ATTACGACTG TGATTATTAT TGCTCCAACC TTTTTGAAGA TCTCACTAAA
    CTGCTTTCCA AGAATGCCTT TCCAGTTTCT GGACAGCTGT ACACCACTCA TCTCCTTTCC
    CTGGAAGCTC TGCTAACTGT CATTGATAGC ACAGAATCAC ACTGCCAGGC TAAAAATCTA
    AGCAATGTGT CACAGGATAA GAAAGAAACT GGAAAACCAA GTGGAGATCT GTCAGAAGGG
    ACCAAAGATT CTGGAAATCT CAATGAACAC TCCATTGAAG TTAAGCCATT CACTGTTAGT
    GCAGAAAACA ATGGATCGAA GCCCCCCACA AGTGGCCACT TAATGGCAGA TAAAATGACT
    CTTGGGGTCC AAGAAACAGA GTACAGCACT GATGGAGGTG AGAAGAAGAC TTTAAAGAAG
    CCCCAACGCT TTTCCTGCTT ACTTCCGAAT GTTCAAGAGC TGAATGACAT TAAGACCAAA
    AAGAAGCTTC TTATAACTGG AACTGAGCAG TTCAACCAGA AACCAAAGAA AGGAGTGCAG
    TTTCTTCAGG AAAAAGACCT TTTGGCAACC CCCATGGATA ATGCTGAGGT AGCACAGTGG
    CTCAGAGAAA ATCCAAGGCT GGACAAGAAA ATGATTGGGG AGTTTGTGAG TGATCGCAAA
    AATCTGGACT TACTTGAAAG TTTTGTAGGA ACCTTCCATT TCCAAGGCCT GAGAGTTGAT
    GAAGCTTTAA GGCTTTACCT TGAAGCTTTT CGTTTGCCTG GCGAGGCACC TGTGATTCAG
    AGGTTACTGG AGGCTTTTAC AGAGCACTGG CGAAAATCCA ATGGAACTCC TTTTGCACAC
    AGTGATGCCT GCTTTGCTTT GGCTTATGCA GTTATCATGC TGAACACAGA CCAACACAAT
    CACAATGTCC GCAAGCAAAA TGTGCCAATG ACTTTAGAGG AATTCAGGAA AAATTTAAAG
    GGTGTTAATG GTGGAAAAGA TTTTGACCAA GATATGCTAG AGGATATTTA TCACGCAATC
    AAAAATGAAG AAATTGTAAT GCCTGAAGAG CAGACTGGCT TAGTAAAAGA GAATTATTTT
    TGGAATGTTT TGCTGCATCG GGGAGCCACT CCAGAAGGAA TGTTCTTGCA TGTGGATCCT
    GGAAGTTATG ACCATGACCT CTTTACTATG ACTTGGGGGC CAACAATAGC TGCCTTGTCC
    TATGTGTTTG ATAAGAGCAT GGATGAAACG ATTATTCAGA AAGCAATATC TGGTTTTAGG
    AAATGTGCAA TGATATCTGC TCACTATGGT CTCAGTGATG TTTTTGACAA TCTCATAATT
    TCCTTGTGCA AGTTTACAAC ACTTAGCAGT GAAGCCGTTG AAAATCTTCC CACTGTTTTT
    GGAAGCAACC TAAAAGCTCA GATCGCTGCA AAGACTGTGT TCCACTTATC CCACAGACAT
    GGTGACATCC TTCGAGAGGG ATGGAAAAAT ATTATGGACT CCATGCTGCA GCTTTTTAGA
    GCTGAGCTGC TTCCCAAGGC TATGGTTGAG GTTGAAGATT TTGTAGACCC CAATGGAAAA
    ATATCTCTTC AACGTGAGGA AATCCCTGCC AACCGTGGGG AATCCACTGT ACTAAGTTTT
    GTCAGCTGGC TGACTCTGAG TGGGACAGAA CAGTCAAGTC TAAGAGGACC TTCTACTGAA
    AATCAAGAGG CCAAGAAACT AGCGCTGGAA TGTATCAAGT TATGTGATCC AGAGAAACTG
    ATCACAGAGA GCAAGTTTCT TCAACTGGAG TCTTTGCAAG AACTAATGAA GGCTCTGATA
    TCTGTGACAC CAGATGAAGA AACGTATGAT GAAGAAGATG CTGCATTCTG CCTAGAAATG
    TTAATGAGAA TTGTGCTAGA AAACAGAGAT CGTGTTGGCT GTGTTTGGCA GGCTGTACGA
    GATCATCTCT ATCGTCTATG TGTCCATGCT GTAGAGTTCT GCTTCCTGGT TGAAAGAGCA
    GTTGTAGGGC TGCTGCGCTT GGCAATACGC CTTTTACGCA GAGAGGAAAT TAGTGGTCAG
    GTTATCCTCT CCTTGAGAAT TCTTTTAATG ATGAAACCAA CTGTGTTGTC TAAAGTGAGT
    CGCCAAGTTG CTTTTGGTGT GCATGAGCTC TTGAAAACCA ATGCTGCTAA CATCCATTCC
    AGCAATGACT GGTACACACT ATTCTGCCTT TTGGAGTGCA TTGGGGCAGG AGTGAAGCCG
    CCCCCTGCCT TACAGGTGAC GACGAGAGCT GACAATGACA CAGGTGCGCA GTCTGACAGT
    GAAGTTTCCT CATACCATCC AAGTGAAGTG AATCTTGATC GTGGATACAC CTCAGACTCT
    GAGGTATATG CAGAACACAG CAAGCAGTCA AAAATGCACC GCTCTGTGAC TGATGTGGAT
    ATGATGAACA GTGGCTGGCT GATGGTTGGA AAGGAAGATG TTGACTCTTC TAAAGTTGCT
    ACAGTTATTA ACAAACCTGT TGCTAATGCC CCAGTGAATC AATACAGCCT AACCATTGGT
    TTGGATTTTG GCCCTCACGA TACCAAATCT CTCATGAAGA GCGTGGAGTC CCTTTCTTTT
    ATTGTTCGAG ATGCTGCACA TGTCACACCA GAAAACTTTG AACTCTGTGT TAAAACAATC
    CGTGTCTTTG TAGAGGCAAG TCTAAATGGA GGTGTGTGGT ACAAATCTCA GGAGAAAAGG
    GTAAAGAACC ACAAATATGA CAGTAAATCC AACCGCTTCA AAAAGAAAAC CAAGGAAAAA
    GAAAATTCTG TCCGCCGTAC ACGGGCTTCA AATCAGCGAC AATACCGATC TCACAGTGAT
    GATGAGGAAG ATGAAAGTGT ACCAGCCAGC TATCACACTG TGTCATTACA GGTTAGTCAT
    GATCTTTTGG ACCTAATGCA CACACTTCAC ACAAGAGCTG CCACCATATA CAGTTCTTGG
    GCAGAGGAAC AGCGCAACCT AGAGACAGCT GCTGAGAAAA TTACAGCCGA TTCTAAGACA
    CTGTGGTCAA ACTGTTGGTG TCCTTTACTG CAAGGTATTG CTTGGCTCTG CTGTGATGCT
    CGGCGCCAGG TCAGAATGCA GGCTCTCACC TACCTGCAAA GGGCACTTTT AGTCCATGAC
    TTACAGGCTC TTGATGCACT TGAATGGGAA TCATGCTTTA ATAAGGTGCT ATTTCCATTG
    CTGACCAAAC TATTGGAAAA TATTAGTCCA GCTGATGTTG GCGGGATGGA GGAGACCAGA
    ATGAGAGGCT CCACTCTGCT CTCAAAGGTA TTTCTACAGC ATCTGTCTCC TCTTCTTTCT
    CTGTCGACAT TTGCTGCCTT GTGGCTTACT ATTCTTGATT TCATGGACAA GTACATGCAT
    GCTGGCTCCA GTGACTTACT GCCTGAGGCA ATTCCAGAGT CACTGAAAAA TATGCTTCTC
    GTAATGGACA CTGCCGGAAT ATTCCACAGT GCAGACTCGC GGACGGGCTA CTCTGATTTA
    TGGGAGATCA CGTGGGAAAG GATAGATTGC TTTTTACCTC GTCTAAGGGA AGAACTGTTT
    AAACAAACTG TGATACAAGA TCCACCCAAT ATAGTTACAA CAGAAACTCC TGTTGAGCCA
    AGACCTCCCC CTCAATCAAC TCAGTCAGAG AGACCAGCGG AGCAGGGACC TTGTGACTCA
    GAGACTCAAA GTTTAGCAAT CTCATCTGCT CCAGTAGTTG GATCAAGTTC TTCCTGCACC
    CCAGTTAGAG TCAGCCCTGT AACAGATATA CCTCCACCTA TAACTCAGTC TCCAGTCATC
    CTGCATCCTC TGACCTCCCC GCTGCAGGTG GGAGTGCCAC CCATGTCGCT GCCAATCATC
    CTCAACCCTG CATTAATTGA AGCTACCTCT CCAGTTCCGC TCTTGTCCAC TCCCCGACCT
    GCTGATGTAT CATCTGTCTC AGAGGTCAAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa62535
    Clone ID Related Accession (Same CDS sequence) XM_031906083.1
    Accession Version XM_031906083.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5667bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGA TCTCTCCATG TCTACTCTGC
    AAAATGGAGC TTAATAGTGG TGAACAGTCC AAAAACCTGA ACCAGTTAGA GAGGGTACTT
    CTTTTTAAGA GCCTGAAGTT GAAAATGAGA GCTGGAGGAA TGAGTGACTC CTCAAAATGG
    AAAAAGCAGA AGAAATCTCC TAGGCAGCCA CGTCATGTTA CCAAAGCTAT GGCGGCAGCA
    GAACCCTCCT CTCAGCAACC AGTAGTTAAT GGCACCACAA TTACTACTAA CCTCACTGGT
    GAAGTTCCTT TTATTGATGC TTCCTCTCCA GATGCTAATT TTCCCATTTC AGGGGTGAGT
    TCAGAGGCCA CGTCTGCTGT AGTCAGTCCT ACCACAGATA GTGGTGTAGA TATGTCCTCT
    CAGGCAACAT CTAAAGAAGA CCTTTCTGAA CTGGATCCTG CAAGTCCATC TCTTTCTGCC
    ATTCAGCAAT CAGGAGACAT TTGTCTTTCT GAACAGCAAA ACCAGGATGA TGATAAGATT
    GAAGACCTTA TGGAAAGGCA TACATATTCT GCTTCTGTGG AATCCATACC AGAAGTATTA
    GATGATAATG TGTCATTGGC TGATCAATCA GACTCCGCTT CAGTCCATGA TATGGATTAT
    GTAAATCCTA GAGGTGTGAG ATTTACCCAA TCTTCACAGA AAGAAGGAAC AGTCTTAGTA
    CCATATGGAC TGCCATGCAT TCGGGAATTA TTTCGTTTTC TTATTTCTTT AACCAACCCC
    CATGATCGAC ATAACTCCGA AGTGATGATA CACATGGGTT TACAGTTGAT CACAGTTGCT
    CTTGAGTCTG CACCTATTGA AAATTATGTT TCGCTTTTAG GCCTTGTAAA GGATGAACTT
    TGTCGACACT TATTTCAGCT ACTGAGCATT GAGAGACTGA ACCTATATGC AGCTTCCTTG
    CGAGCATGTT TTCTTTTGTT TGAGGGTATG AGGGAGCATT TGAAGTTCCA GCTAGAGATG
    TACATAAAGA AGCTCATGGA TATTATTACT GTGGAGAACC CTAAGATGCC TTATGAGATG
    AAAGAAATGG CTCTTGAAGC TATTGTGCAA TTGTGGAGAA TCCCGAGCTT TGTTACAGAA
    TTATACATTA ATTACGACTG TGATTATTAT TGCTCCAACC TTTTTGAAGA TCTCACTAAA
    CTGCTTTCCA AGAATGCCTT TCCAGTTTCT GGACAGCTGT ACACCACTCA TCTCCTTTCC
    CTGGAAGCTC TGCTAACTGT CATTGATAGC ACAGAATCAC ACTGCCAGGC TAAAAATCTA
    AGCAATGTGT CACAGGATAA GAAAGAAACT GGAAAACCAA GTGGAGATCT GTCAGAAGGG
    ACCAAAGATT CTGGAAATCT CAATGAACAC TCCATTGAAG TTAAGCCATT CACTGTTAGT
    GCAGAAAACA ATGGATCGAA GCCCCCCACA AGTGGCCACT TAATGGCAGA TAAAATGACT
    CTTGGGGTCC AAGAAACAGA GTACAGCACT GATGGAGGTG AGAAGAAGAC TTTAAAGAAG
    CCCCAACGCT TTTCCTGCTT ACTTCCGAAT GTTCAAGAGC TGAATGACAT TAAGACCAAA
    AAGAAGCTTC TTATAACTGG AACTGAGCAG TTCAACCAGA AACCAAAGAA AGGAGTGCAG
    TTTCTTCAGG AAAAAGACCT TTTGGCAACC CCCATGGATA ATGCTGAGGT AGCACAGTGG
    CTCAGAGAAA ATCCAAGGCT GGACAAGAAA ATGATTGGGG AGTTTGTGAG TGATCGCAAA
    AATCTGGACT TACTTGAAAG TTTTGTAGGA ACCTTCCATT TCCAAGGCCT GAGAGTTGAT
    GAAGCTTTAA GGCTTTACCT TGAAGCTTTT CGTTTGCCTG GCGAGGCACC TGTGATTCAG
    AGGTTACTGG AGGCTTTTAC AGAGCACTGG CGAAAATCCA ATGGAACTCC TTTTGCACAC
    AGTGATGCCT GCTTTGCTTT GGCTTATGCA GTTATCATGC TGAACACAGA CCAACACAAT
    CACAATGTCC GCAAGCAAAA TGTGCCAATG ACTTTAGAGG AATTCAGGAA AAATTTAAAG
    GGTGTTAATG GTGGAAAAGA TTTTGACCAA GATATGCTAG AGGATATTTA TCACGCAATC
    AAAAATGAAG AAATTGTAAT GCCTGAAGAG CAGACTGGCT TAGTAAAAGA GAATTATTTT
    TGGAATGTTT TGCTGCATCG GGGAGCCACT CCAGAAGGAA TGTTCTTGCA TGTGGATCCT
    GGAAGTTATG ACCATGACCT CTTTACTATG ACTTGGGGGC CAACAATAGC TGCCTTGTCC
    TATGTGTTTG ATAAGAGCAT GGATGAAACG ATTATTCAGA AAGCAATATC TGGTTTTAGG
    AAATGTGCAA TGATATCTGC TCACTATGGT CTCAGTGATG TTTTTGACAA TCTCATAATT
    TCCTTGTGCA AGTTTACAAC ACTTAGCAGT GAAGCCGTTG AAAATCTTCC CACTGTTTTT
    GGAAGCAACC TAAAAGCTCA GATCGCTGCA AAGACTGTGT TCCACTTATC CCACAGACAT
    GGTGACATCC TTCGAGAGGG ATGGAAAAAT ATTATGGACT CCATGCTGCA GCTTTTTAGA
    GCTGAGCTGC TTCCCAAGGC TATGGTTGAG GTTGAAGATT TTGTAGACCC CAATGGAAAA
    ATATCTCTTC AACGTGAGGA AATCCCTGCC AACCGTGGGG AATCCACTGT ACTAAGTTTT
    GTCAGCTGGC TGACTCTGAG TGGGACAGAA CAGTCAAGTC TAAGAGGACC TTCTACTGAA
    AATCAAGAGG CCAAGAAACT AGCGCTGGAA TGTATCAAGT TATGTGATCC AGAGAAACTG
    ATCACAGAGA GCAAGTTTCT TCAACTGGAG TCTTTGCAAG AACTAATGAA GGCTCTGATA
    TCTGTGACAC CAGATGAAGA AACGTATGAT GAAGAAGATG CTGCATTCTG CCTAGAAATG
    TTAATGAGAA TTGTGCTAGA AAACAGAGAT CGTGTTGGCT GTGTTTGGCA GGCTGTACGA
    GATCATCTCT ATCGTCTATG TGTCCATGCT GTAGAGTTCT GCTTCCTGGT TGAAAGAGCA
    GTTGTAGGGC TGCTGCGCTT GGCAATACGC CTTTTACGCA GAGAGGAAAT TAGTGGTCAG
    GTTATCCTCT CCTTGAGAAT TCTTTTAATG ATGAAACCAA CTGTGTTGTC TAAAGTGAGT
    CGCCAAGTTG CTTTTGGTGT GCATGAGCTC TTGAAAACCA ATGCTGCTAA CATCCATTCC
    AGCAATGACT GGTACACACT ATTCTGCCTT TTGGAGTGCA TTGGGGCAGG AGTGAAGCCG
    CCCCCTGCCT TACAGGTGAC GACGAGAGCT GACAATGACA CAGGTGCGCA GTCTGACAGT
    GAAGTTTCCT CATACCATCC AAGTGAAGTG AATCTTGATC GTGGATACAC CTCAGACTCT
    GAGGTATATG CAGAACACAG CAAGCAGTCA AAAATGCACC GCTCTGTGAC TGATGTGGAT
    ATGATGAACA GTGGCTGGCT GATGGTTGGA AAGGAAGATG TTGACTCTTC TAAAGTTGCT
    ACAGTTATTA ACAAACCTGT TGCTAATGCC CCAGTGAATC AATACAGCCT AACCATTGGT
    TTGGATTTTG GCCCTCACGA TACCAAATCT CTCATGAAGA GCGTGGAGTC CCTTTCTTTT
    ATTGTTCGAG ATGCTGCACA TGTCACACCA GAAAACTTTG AACTCTGTGT TAAAACAATC
    CGTGTCTTTG TAGAGGCAAG TCTAAATGGA GGGTACAAAT CTCAGGAGAA AAGGGTAAAG
    AACCACAAAT ATGACAGTAA ATCCAACCGC TTCAAAAAGA AAACCAAGGA AAAAGAAAAT
    TCTGTCCGCC GTACACGGGC TTCAAATCAG CGACAATACC GATCTCACAG TGATGATGAG
    GAAGATGAAA GTGTACCAGC CAGCTATCAC ACTGTGTCAT TACAGGTTAG TCATGATCTT
    TTGGACCTAA TGCACACACT TCACACAAGA GCTGCCACCA TATACAGTTC TTGGGCAGAG
    GAACAGCGCA ACCTAGAGAC AGCTGCTGAG AAAATTACAG CCGATTCTAA GACACTGTGG
    TCAAACTGTT GGTGTCCTTT ACTGCAAGGT ATTGCTTGGC TCTGCTGTGA TGCTCGGCGC
    CAGGTCAGAA TGCAGGCTCT CACCTACCTG CAAAGGGCAC TTTTAGTCCA TGACTTACAG
    GCTCTTGATG CACTTGAATG GGAATCATGC TTTAATAAGG TGCTATTTCC ATTGCTGACC
    AAACTATTGG AAAATATTAG TCCAGCTGAT GTTGGCGGGA TGGAGGAGAC CAGAATGAGA
    GGCTCCACTC TGCTCTCAAA GGTATTTCTA CAGCATCTGT CTCCTCTTCT TTCTCTGTCG
    ACATTTGCTG CCTTGTGGCT TACTATTCTT GATTTCATGG ACAAGTACAT GCATGCTGGC
    TCCAGTGACT TACTGCCTGA GGCAATTCCA GAGTCACTGA AAAATATGCT TCTCGTAATG
    GACACTGCCG GAATATTCCA CAGTGCAGAC TCGCGGACGG GCTACTCTGA TTTATGGGAG
    ATCACGTGGG AAAGGATAGA TTGCTTTTTA CCTCGTCTAA GGGAAGAACT GTTTAAACAA
    ACTGTGATAC AAGATCCACC CAATATAGTT ACAACAGAAA CTCCTGTTGA GCCAAGACCT
    CCCCCTCAAT CAACTCAGTC AGAGAGACCA GCGGAGCAGG GACCTTGTGA CTCAGAGACT
    CAAAGTTTAG CAATCTCATC TGCTCCAGTA GTTGGATCAA GTTCTTCCTG CACCCCAGTT
    AGAGTCAGCC CTGTAACAGA TATACCTCCA CCTATAACTC AGTCTCCAGT CATCCTGCAT
    CCTCTGACCT CCCCGCTGCA GGTGGGAGTG CCACCCATGT CGCTGCCAAT CATCCTCAAC
    CCTGCATTAA TTGAAGCTAC CTCTCCAGTT CCGCTCTTGT CCACTCCCCG ACCTGCTGAT
    GTATCATCTG TCTCAGAGGT CAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031761943.1
    CDS100..5766
    Translation

    Target ORF information:

    RefSeq Version XM_031906083.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031906083.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGA TCTCTCCATG TCTACTCTGC
    AAAATGGAGC TTAATAGTGG TGAACAGTCC AAAAACCTGA ACCAGTTAGA GAGGGTACTT
    CTTTTTAAGA GCCTGAAGTT GAAAATGAGA GCTGGAGGAA TGAGTGACTC CTCAAAATGG
    AAAAAGCAGA AGAAATCTCC TAGGCAGCCA CGTCATGTTA CCAAAGCTAT GGCGGCAGCA
    GAACCCTCCT CTCAGCAACC AGTAGTTAAT GGCACCACAA TTACTACTAA CCTCACTGGT
    GAAGTTCCTT TTATTGATGC TTCCTCTCCA GATGCTAATT TTCCCATTTC AGGGGTGAGT
    TCAGAGGCCA CGTCTGCTGT AGTCAGTCCT ACCACAGATA GTGGTGTAGA TATGTCCTCT
    CAGGCAACAT CTAAAGAAGA CCTTTCTGAA CTGGATCCTG CAAGTCCATC TCTTTCTGCC
    ATTCAGCAAT CAGGAGACAT TTGTCTTTCT GAACAGCAAA ACCAGGATGA TGATAAGATT
    GAAGACCTTA TGGAAAGGCA TACATATTCT GCTTCTGTGG AATCCATACC AGAAGTATTA
    GATGATAATG TGTCATTGGC TGATCAATCA GACTCCGCTT CAGTCCATGA TATGGATTAT
    GTAAATCCTA GAGGTGTGAG ATTTACCCAA TCTTCACAGA AAGAAGGAAC AGTCTTAGTA
    CCATATGGAC TGCCATGCAT TCGGGAATTA TTTCGTTTTC TTATTTCTTT AACCAACCCC
    CATGATCGAC ATAACTCCGA AGTGATGATA CACATGGGTT TACAGTTGAT CACAGTTGCT
    CTTGAGTCTG CACCTATTGA AAATTATGTT TCGCTTTTAG GCCTTGTAAA GGATGAACTT
    TGTCGACACT TATTTCAGCT ACTGAGCATT GAGAGACTGA ACCTATATGC AGCTTCCTTG
    CGAGCATGTT TTCTTTTGTT TGAGGGTATG AGGGAGCATT TGAAGTTCCA GCTAGAGATG
    TACATAAAGA AGCTCATGGA TATTATTACT GTGGAGAACC CTAAGATGCC TTATGAGATG
    AAAGAAATGG CTCTTGAAGC TATTGTGCAA TTGTGGAGAA TCCCGAGCTT TGTTACAGAA
    TTATACATTA ATTACGACTG TGATTATTAT TGCTCCAACC TTTTTGAAGA TCTCACTAAA
    CTGCTTTCCA AGAATGCCTT TCCAGTTTCT GGACAGCTGT ACACCACTCA TCTCCTTTCC
    CTGGAAGCTC TGCTAACTGT CATTGATAGC ACAGAATCAC ACTGCCAGGC TAAAAATCTA
    AGCAATGTGT CACAGGATAA GAAAGAAACT GGAAAACCAA GTGGAGATCT GTCAGAAGGG
    ACCAAAGATT CTGGAAATCT CAATGAACAC TCCATTGAAG TTAAGCCATT CACTGTTAGT
    GCAGAAAACA ATGGATCGAA GCCCCCCACA AGTGGCCACT TAATGGCAGA TAAAATGACT
    CTTGGGGTCC AAGAAACAGA GTACAGCACT GATGGAGGTG AGAAGAAGAC TTTAAAGAAG
    CCCCAACGCT TTTCCTGCTT ACTTCCGAAT GTTCAAGAGC TGAATGACAT TAAGACCAAA
    AAGAAGCTTC TTATAACTGG AACTGAGCAG TTCAACCAGA AACCAAAGAA AGGAGTGCAG
    TTTCTTCAGG AAAAAGACCT TTTGGCAACC CCCATGGATA ATGCTGAGGT AGCACAGTGG
    CTCAGAGAAA ATCCAAGGCT GGACAAGAAA ATGATTGGGG AGTTTGTGAG TGATCGCAAA
    AATCTGGACT TACTTGAAAG TTTTGTAGGA ACCTTCCATT TCCAAGGCCT GAGAGTTGAT
    GAAGCTTTAA GGCTTTACCT TGAAGCTTTT CGTTTGCCTG GCGAGGCACC TGTGATTCAG
    AGGTTACTGG AGGCTTTTAC AGAGCACTGG CGAAAATCCA ATGGAACTCC TTTTGCACAC
    AGTGATGCCT GCTTTGCTTT GGCTTATGCA GTTATCATGC TGAACACAGA CCAACACAAT
    CACAATGTCC GCAAGCAAAA TGTGCCAATG ACTTTAGAGG AATTCAGGAA AAATTTAAAG
    GGTGTTAATG GTGGAAAAGA TTTTGACCAA GATATGCTAG AGGATATTTA TCACGCAATC
    AAAAATGAAG AAATTGTAAT GCCTGAAGAG CAGACTGGCT TAGTAAAAGA GAATTATTTT
    TGGAATGTTT TGCTGCATCG GGGAGCCACT CCAGAAGGAA TGTTCTTGCA TGTGGATCCT
    GGAAGTTATG ACCATGACCT CTTTACTATG ACTTGGGGGC CAACAATAGC TGCCTTGTCC
    TATGTGTTTG ATAAGAGCAT GGATGAAACG ATTATTCAGA AAGCAATATC TGGTTTTAGG
    AAATGTGCAA TGATATCTGC TCACTATGGT CTCAGTGATG TTTTTGACAA TCTCATAATT
    TCCTTGTGCA AGTTTACAAC ACTTAGCAGT GAAGCCGTTG AAAATCTTCC CACTGTTTTT
    GGAAGCAACC TAAAAGCTCA GATCGCTGCA AAGACTGTGT TCCACTTATC CCACAGACAT
    GGTGACATCC TTCGAGAGGG ATGGAAAAAT ATTATGGACT CCATGCTGCA GCTTTTTAGA
    GCTGAGCTGC TTCCCAAGGC TATGGTTGAG GTTGAAGATT TTGTAGACCC CAATGGAAAA
    ATATCTCTTC AACGTGAGGA AATCCCTGCC AACCGTGGGG AATCCACTGT ACTAAGTTTT
    GTCAGCTGGC TGACTCTGAG TGGGACAGAA CAGTCAAGTC TAAGAGGACC TTCTACTGAA
    AATCAAGAGG CCAAGAAACT AGCGCTGGAA TGTATCAAGT TATGTGATCC AGAGAAACTG
    ATCACAGAGA GCAAGTTTCT TCAACTGGAG TCTTTGCAAG AACTAATGAA GGCTCTGATA
    TCTGTGACAC CAGATGAAGA AACGTATGAT GAAGAAGATG CTGCATTCTG CCTAGAAATG
    TTAATGAGAA TTGTGCTAGA AAACAGAGAT CGTGTTGGCT GTGTTTGGCA GGCTGTACGA
    GATCATCTCT ATCGTCTATG TGTCCATGCT GTAGAGTTCT GCTTCCTGGT TGAAAGAGCA
    GTTGTAGGGC TGCTGCGCTT GGCAATACGC CTTTTACGCA GAGAGGAAAT TAGTGGTCAG
    GTTATCCTCT CCTTGAGAAT TCTTTTAATG ATGAAACCAA CTGTGTTGTC TAAAGTGAGT
    CGCCAAGTTG CTTTTGGTGT GCATGAGCTC TTGAAAACCA ATGCTGCTAA CATCCATTCC
    AGCAATGACT GGTACACACT ATTCTGCCTT TTGGAGTGCA TTGGGGCAGG AGTGAAGCCG
    CCCCCTGCCT TACAGGTGAC GACGAGAGCT GACAATGACA CAGGTGCGCA GTCTGACAGT
    GAAGTTTCCT CATACCATCC AAGTGAAGTG AATCTTGATC GTGGATACAC CTCAGACTCT
    GAGGTATATG CAGAACACAG CAAGCAGTCA AAAATGCACC GCTCTGTGAC TGATGTGGAT
    ATGATGAACA GTGGCTGGCT GATGGTTGGA AAGGAAGATG TTGACTCTTC TAAAGTTGCT
    ACAGTTATTA ACAAACCTGT TGCTAATGCC CCAGTGAATC AATACAGCCT AACCATTGGT
    TTGGATTTTG GCCCTCACGA TACCAAATCT CTCATGAAGA GCGTGGAGTC CCTTTCTTTT
    ATTGTTCGAG ATGCTGCACA TGTCACACCA GAAAACTTTG AACTCTGTGT TAAAACAATC
    CGTGTCTTTG TAGAGGCAAG TCTAAATGGA GGGTACAAAT CTCAGGAGAA AAGGGTAAAG
    AACCACAAAT ATGACAGTAA ATCCAACCGC TTCAAAAAGA AAACCAAGGA AAAAGAAAAT
    TCTGTCCGCC GTACACGGGC TTCAAATCAG CGACAATACC GATCTCACAG TGATGATGAG
    GAAGATGAAA GTGTACCAGC CAGCTATCAC ACTGTGTCAT TACAGGTTAG TCATGATCTT
    TTGGACCTAA TGCACACACT TCACACAAGA GCTGCCACCA TATACAGTTC TTGGGCAGAG
    GAACAGCGCA ACCTAGAGAC AGCTGCTGAG AAAATTACAG CCGATTCTAA GACACTGTGG
    TCAAACTGTT GGTGTCCTTT ACTGCAAGGT ATTGCTTGGC TCTGCTGTGA TGCTCGGCGC
    CAGGTCAGAA TGCAGGCTCT CACCTACCTG CAAAGGGCAC TTTTAGTCCA TGACTTACAG
    GCTCTTGATG CACTTGAATG GGAATCATGC TTTAATAAGG TGCTATTTCC ATTGCTGACC
    AAACTATTGG AAAATATTAG TCCAGCTGAT GTTGGCGGGA TGGAGGAGAC CAGAATGAGA
    GGCTCCACTC TGCTCTCAAA GGTATTTCTA CAGCATCTGT CTCCTCTTCT TTCTCTGTCG
    ACATTTGCTG CCTTGTGGCT TACTATTCTT GATTTCATGG ACAAGTACAT GCATGCTGGC
    TCCAGTGACT TACTGCCTGA GGCAATTCCA GAGTCACTGA AAAATATGCT TCTCGTAATG
    GACACTGCCG GAATATTCCA CAGTGCAGAC TCGCGGACGG GCTACTCTGA TTTATGGGAG
    ATCACGTGGG AAAGGATAGA TTGCTTTTTA CCTCGTCTAA GGGAAGAACT GTTTAAACAA
    ACTGTGATAC AAGATCCACC CAATATAGTT ACAACAGAAA CTCCTGTTGA GCCAAGACCT
    CCCCCTCAAT CAACTCAGTC AGAGAGACCA GCGGAGCAGG GACCTTGTGA CTCAGAGACT
    CAAAGTTTAG CAATCTCATC TGCTCCAGTA GTTGGATCAA GTTCTTCCTG CACCCCAGTT
    AGAGTCAGCC CTGTAACAGA TATACCTCCA CCTATAACTC AGTCTCCAGT CATCCTGCAT
    CCTCTGACCT CCCCGCTGCA GGTGGGAGTG CCACCCATGT CGCTGCCAAT CATCCTCAAC
    CCTGCATTAA TTGAAGCTAC CTCTCCAGTT CCGCTCTTGT CCACTCCCCG ACCTGCTGAT
    GTATCATCTG TCTCAGAGGT CAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa62536
    Clone ID Related Accession (Same CDS sequence) XM_031906084.1
    Accession Version XM_031906084.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5661bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGA TCTCTCCATG TCTACTCTGC
    AAAATGGAGC TTAATAGTGG TGAACAGTCC AAAAACCTGA ACCAGTTAGA GAGGGTACTT
    CTTTTTAAGA GCCTGAAGTT GAAAATGAGA GCTGGAGGAA TGAGTGACTC CTCAAAATGG
    AAAAAGCAGA AGAAATCTCC TAGGCAGCCA CGTCATGTTA CCAAAGCTAT GGCGGCAGCA
    GAACCCTCCT CTCAGCAACC AGTAGTTAAT GGCACCACAA TTACTACTAA CCTCACTGGT
    GAAGTTCCTT TTATTGATGC TTCCTCTCCA GATGCTAATT TTCCCATTTC AGGGGTGAGT
    TCAGAGGCCA CGTCTGCTGT AGTCAGTCCT ACCACAGATA GTGGTGTAGA TATGTCCTCT
    CAGGCAACAT CTAAAGAAGA CCTTTCTGAA CTGGATCCTG CAAGTCCATC TCTTTCTGCC
    ATTCAGCAAT CAGGAGACAT TTGTCTTTCT GAACAGCAAA ACCAGGATGA TGATAAGATT
    GAAGACCTTA TGGAAAGGCA TACATATTCT GCTTCTGTGG AATCCATACC AGAAGTATTA
    GATGATAATG TGTCATTGGC TGATCAATCA GACTCCGCTT CAGTCCATGA TATGGATTAT
    GTAAATCCTA GAGGTGTGAG ATTTACCCAA TCTTCACAGA AAGAAGGAAC AGTCTTAGTA
    CCATATGGAC TGCCATGCAT TCGGGAATTA TTTCGTTTTC TTATTTCTTT AACCAACCCC
    CATGATCGAC ATAACTCCGA AGTGATGATA CACATGGGTT TACAGTTGAT CACAGTTGCT
    CTTGAGTCTG CACCTATTGA AAATTATGTT TCGCTTTTAG GCCTTGTAAA GGATGAACTT
    TGTCGACACT TATTTCAGCT ACTGAGCATT GAGAGACTGA ACCTATATGC AGCTTCCTTG
    CGAGCATGTT TTCTTTTGTT TGAGGGTATG AGGGAGCATT TGAAGTTCCA GCTAGAGATG
    TACATAAAGA AGCTCATGGA TATTATTACT GTGGAGAACC CTAAGATGCC TTATGAGATG
    AAAGAAATGG CTCTTGAAGC TATTGTGCAA TTGTGGAGAA TCCCGAGCTT TGTTACAGAA
    TTATACATTA ATTACGACTG TGATTATTAT TGCTCCAACC TTTTTGAAGA TCTCACTAAA
    CTGCTTTCCA AGAATGCCTT TCCAGTTTCT GGACAGCTGT ACACCACTCA TCTCCTTTCC
    CTGGAAGCTC TGCTAACTGT CATTGATAGC ACAGAATCAC ACTGCCAGGC TAAAAATCTA
    AGCAATGTGT CACAGGATAA GAAAGAAACT GGAAAACCAA GTGGAGATCT GTCAGAAGGG
    ACCAAAGATT CTGGAAATCT CAATGAACAC TCCATTGAAG TTAAGCCATT CACTGTTAGT
    GCAGAAAACA ATGGATCGAA GCCCCCCACA AGTGGCCACT TAATGGCAGA TAAAATGACT
    CTTGGGGTCC AAGAAACAGA GTACAGCACT GATGGAGGTG AGAAGAAGAC TTTAAAGAAG
    CCCCAACGCT TTTCCTGCTT ACTTCCGAAT GTTCAAGAGC TGAATGACAT TAAGACCAAA
    AAGAAGCTTC TTATAACTGG AACTGAGCAG TTCAACCAGA AACCAAAGAA AGGAGTGCAG
    TTTCTTCAGG AAAAAGACCT TTTGGCAACC CCCATGGATA ATGCTGAGGT AGCACAGTGG
    CTCAGAGAAA ATCCAAGGCT GGACAAGAAA ATGATTGGGG AGTTTGTGAG TGATCGCAAA
    AATCTGGACT TACTTGAAAG TTTTGTAGGA ACCTTCCATT TCCAAGGCCT GAGAGTTGAT
    GAAGCTTTAA GGCTTTACCT TGAAGCTTTT CGTTTGCCTG GCGAGGCACC TGTGATTCAG
    AGGTTACTGG AGGCTTTTAC AGAGCACTGG CGAAAATCCA ATGGAACTCC TTTTGCACAC
    AGTGATGCCT GCTTTGCTTT GGCTTATGCA GTTATCATGC TGAACACAGA CCAACACAAT
    CACAATGTCC GCAAGCAAAA TGTGCCAATG ACTTTAGAGG AATTCAGGAA AAATTTAAAG
    GGTGTTAATG GTGGAAAAGA TTTTGACCAA GATATGCTAG AGGATATTTA TCACGCAATC
    AAAAATGAAG AAATTGTAAT GCCTGAAGAG CAGACTGGCT TAGTAAAAGA GAATTATTTT
    TGGAATGTTT TGCTGCATCG GGGAGCCACT CCAGAAGGAA TGTTCTTGCA TGTGGATCCT
    GGAAGTTATG ACCATGACCT CTTTACTATG ACTTGGGGGC CAACAATAGC TGCCTTGTCC
    TATGTGTTTG ATAAGAGCAT GGATGAAACG ATTATTCAGA AAGCAATATC TGGTTTTAGG
    AAATGTGCAA TGATATCTGC TCACTATGGT CTCAGTGATG TTTTTGACAA TCTCATAATT
    TCCTTGTGCA AGTTTACAAC ACTTAGCAGT GAAGCCGTTG AAAATCTTCC CACTGTTTTT
    GGAAGCAACC TAAAAGCTCA GATCGCTGCA AAGACTGTGT TCCACTTATC CCACAGACAT
    GGTGACATCC TTCGAGAGGG ATGGAAAAAT ATTATGGACT CCATGCTGCA GCTTTTTAGA
    GCTGAGCTGC TTCCCAAGGC TATGGTTGAG GTTGAAGATT TTGTAGACCC CAATGGAAAA
    ATATCTCTTC AACGTGAGGA AATCCCTGCC AACCGTGGGG AATCCACTGT ACTAAGTTTT
    GTCAGCTGGC TGACTCTGAG TGGGACAGAA CAGTCAAGTC TAAGAGGACC TTCTACTGAA
    AATCAAGAGG CCAAGAAACT AGCGCTGGAA TGTATCAAGT TATGTGATCC AGAGAAACTG
    ATCACAGAGA GCAAGTTTCT TCAACTGGAG TCTTTGCAAG AACTAATGAA GGCTCTGATA
    TCTGTGACAC CAGATGAAGA AACGTATGAT GAAGAAGATG CTGCATTCTG CCTAGAAATG
    TTAATGAGAA TTGTGCTAGA AAACAGAGAT CGTGTTGGCT GTGTTTGGCA GGCTGTACGA
    GATCATCTCT ATCGTCTATG TGTCCATGCT GTAGAGTTCT GCTTCCTGGT TGAAAGAGCA
    GTTGTAGGGC TGCTGCGCTT GGCAATACGC CTTTTACGCA GAGAGGAAAT TAGTGGTCAG
    GTTATCCTCT CCTTGAGAAT TCTTTTAATG ATGAAACCAA CTGTGTTGTC TAAAGTGAGT
    CGCCAAGTTG CTTTTGGTGT GCATGAGCTC TTGAAAACCA ATGCTGCTAA CATCCATTCC
    AGCAATGACT GGTACACACT ATTCTGCCTT TTGGAGTGCA TTGGGGCAGG AGTGAAGCCG
    CCCCCTGCCT TACAGGTGAC GACGAGAGCT GACAATGACA CAGGTGCGCA GTCTGACAGT
    GAAGTTTCCT CATACCATCC AAGTGAAGTG AATCTTGATC GTGGATACAC CTCAGACTCT
    GAGGTATATG CAGAACACAG CAAGCAGTCA AAAATGCACC GCTCTGTGAC TGATGTGGAT
    ATGATGAACA GTGGCTGGCT GATGGTTGGA AAGGAAGATG TTGACTCTTC TAAAGTTGCT
    ACAGTTATTA ACAAACCTGT TGCTAATGCC CCAGTGAATC AATACAGCCT AACCATTGGT
    TTGGATTTTG GCCCTCACGA TACCAAATCT CTCATGAAGA GCGTGGAGTC CCTTTCTTTT
    ATTGTTCGAG ATGCTGCACA TGTCACACCA GAAAACTTTG AACTCTGTGT TAAAACAATC
    CGTGTCTTTG TAGAGGCAAG TCTAAATGGA GGTGTGTGGT ACAAATCTCA GGAGAAAAGG
    GTAAAGAACC ACAAATATGA CAGTAAATCC AACCGCTTCA AAAAGAAAAC CAAGGAAAAA
    GAAAATTCTG TCCGCCGTAC ACGGGCTTCA AATCAGCGAC AATACCGATC TCACAGTGAT
    GATGAGGAAG ATGAAAGTGT ACCAGCCAGC TATCACACTG TGTCATTACA GCTTTTGGAC
    CTAATGCACA CACTTCACAC AAGAGCTGCC ACCATATACA GTTCTTGGGC AGAGGAACAG
    CGCAACCTAG AGACAGCTGC TGAGAAAATT ACAGCCGATT CTAAGACACT GTGGTCAAAC
    TGTTGGTGTC CTTTACTGCA AGGTATTGCT TGGCTCTGCT GTGATGCTCG GCGCCAGGTC
    AGAATGCAGG CTCTCACCTA CCTGCAAAGG GCACTTTTAG TCCATGACTT ACAGGCTCTT
    GATGCACTTG AATGGGAATC ATGCTTTAAT AAGGTGCTAT TTCCATTGCT GACCAAACTA
    TTGGAAAATA TTAGTCCAGC TGATGTTGGC GGGATGGAGG AGACCAGAAT GAGAGGCTCC
    ACTCTGCTCT CAAAGGTATT TCTACAGCAT CTGTCTCCTC TTCTTTCTCT GTCGACATTT
    GCTGCCTTGT GGCTTACTAT TCTTGATTTC ATGGACAAGT ACATGCATGC TGGCTCCAGT
    GACTTACTGC CTGAGGCAAT TCCAGAGTCA CTGAAAAATA TGCTTCTCGT AATGGACACT
    GCCGGAATAT TCCACAGTGC AGACTCGCGG ACGGGCTACT CTGATTTATG GGAGATCACG
    TGGGAAAGGA TAGATTGCTT TTTACCTCGT CTAAGGGAAG AACTGTTTAA ACAAACTGTG
    ATACAAGATC CACCCAATAT AGTTACAACA GAAACTCCTG TTGAGCCAAG ACCTCCCCCT
    CAATCAACTC AGTCAGAGAG ACCAGCGGAG CAGGGACCTT GTGACTCAGA GACTCAAAGT
    TTAGCAATCT CATCTGCTCC AGTAGTTGGA TCAAGTTCTT CCTGCACCCC AGTTAGAGTC
    AGCCCTGTAA CAGATATACC TCCACCTATA ACTCAGTCTC CAGTCATCCT GCATCCTCTG
    ACCTCCCCGC TGCAGGTGGG AGTGCCACCC ATGTCGCTGC CAATCATCCT CAACCCTGCA
    TTAATTGAAG CTACCTCTCC AGTTCCGCTC TTGTCCACTC CCCGACCTGC TGATGTATCA
    TCTGTCTCAG AGGTCAACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031761944.1
    CDS100..5760
    Translation

    Target ORF information:

    RefSeq Version XM_031906084.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031906084.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGA TCTCTCCATG TCTACTCTGC
    AAAATGGAGC TTAATAGTGG TGAACAGTCC AAAAACCTGA ACCAGTTAGA GAGGGTACTT
    CTTTTTAAGA GCCTGAAGTT GAAAATGAGA GCTGGAGGAA TGAGTGACTC CTCAAAATGG
    AAAAAGCAGA AGAAATCTCC TAGGCAGCCA CGTCATGTTA CCAAAGCTAT GGCGGCAGCA
    GAACCCTCCT CTCAGCAACC AGTAGTTAAT GGCACCACAA TTACTACTAA CCTCACTGGT
    GAAGTTCCTT TTATTGATGC TTCCTCTCCA GATGCTAATT TTCCCATTTC AGGGGTGAGT
    TCAGAGGCCA CGTCTGCTGT AGTCAGTCCT ACCACAGATA GTGGTGTAGA TATGTCCTCT
    CAGGCAACAT CTAAAGAAGA CCTTTCTGAA CTGGATCCTG CAAGTCCATC TCTTTCTGCC
    ATTCAGCAAT CAGGAGACAT TTGTCTTTCT GAACAGCAAA ACCAGGATGA TGATAAGATT
    GAAGACCTTA TGGAAAGGCA TACATATTCT GCTTCTGTGG AATCCATACC AGAAGTATTA
    GATGATAATG TGTCATTGGC TGATCAATCA GACTCCGCTT CAGTCCATGA TATGGATTAT
    GTAAATCCTA GAGGTGTGAG ATTTACCCAA TCTTCACAGA AAGAAGGAAC AGTCTTAGTA
    CCATATGGAC TGCCATGCAT TCGGGAATTA TTTCGTTTTC TTATTTCTTT AACCAACCCC
    CATGATCGAC ATAACTCCGA AGTGATGATA CACATGGGTT TACAGTTGAT CACAGTTGCT
    CTTGAGTCTG CACCTATTGA AAATTATGTT TCGCTTTTAG GCCTTGTAAA GGATGAACTT
    TGTCGACACT TATTTCAGCT ACTGAGCATT GAGAGACTGA ACCTATATGC AGCTTCCTTG
    CGAGCATGTT TTCTTTTGTT TGAGGGTATG AGGGAGCATT TGAAGTTCCA GCTAGAGATG
    TACATAAAGA AGCTCATGGA TATTATTACT GTGGAGAACC CTAAGATGCC TTATGAGATG
    AAAGAAATGG CTCTTGAAGC TATTGTGCAA TTGTGGAGAA TCCCGAGCTT TGTTACAGAA
    TTATACATTA ATTACGACTG TGATTATTAT TGCTCCAACC TTTTTGAAGA TCTCACTAAA
    CTGCTTTCCA AGAATGCCTT TCCAGTTTCT GGACAGCTGT ACACCACTCA TCTCCTTTCC
    CTGGAAGCTC TGCTAACTGT CATTGATAGC ACAGAATCAC ACTGCCAGGC TAAAAATCTA
    AGCAATGTGT CACAGGATAA GAAAGAAACT GGAAAACCAA GTGGAGATCT GTCAGAAGGG
    ACCAAAGATT CTGGAAATCT CAATGAACAC TCCATTGAAG TTAAGCCATT CACTGTTAGT
    GCAGAAAACA ATGGATCGAA GCCCCCCACA AGTGGCCACT TAATGGCAGA TAAAATGACT
    CTTGGGGTCC AAGAAACAGA GTACAGCACT GATGGAGGTG AGAAGAAGAC TTTAAAGAAG
    CCCCAACGCT TTTCCTGCTT ACTTCCGAAT GTTCAAGAGC TGAATGACAT TAAGACCAAA
    AAGAAGCTTC TTATAACTGG AACTGAGCAG TTCAACCAGA AACCAAAGAA AGGAGTGCAG
    TTTCTTCAGG AAAAAGACCT TTTGGCAACC CCCATGGATA ATGCTGAGGT AGCACAGTGG
    CTCAGAGAAA ATCCAAGGCT GGACAAGAAA ATGATTGGGG AGTTTGTGAG TGATCGCAAA
    AATCTGGACT TACTTGAAAG TTTTGTAGGA ACCTTCCATT TCCAAGGCCT GAGAGTTGAT
    GAAGCTTTAA GGCTTTACCT TGAAGCTTTT CGTTTGCCTG GCGAGGCACC TGTGATTCAG
    AGGTTACTGG AGGCTTTTAC AGAGCACTGG CGAAAATCCA ATGGAACTCC TTTTGCACAC
    AGTGATGCCT GCTTTGCTTT GGCTTATGCA GTTATCATGC TGAACACAGA CCAACACAAT
    CACAATGTCC GCAAGCAAAA TGTGCCAATG ACTTTAGAGG AATTCAGGAA AAATTTAAAG
    GGTGTTAATG GTGGAAAAGA TTTTGACCAA GATATGCTAG AGGATATTTA TCACGCAATC
    AAAAATGAAG AAATTGTAAT GCCTGAAGAG CAGACTGGCT TAGTAAAAGA GAATTATTTT
    TGGAATGTTT TGCTGCATCG GGGAGCCACT CCAGAAGGAA TGTTCTTGCA TGTGGATCCT
    GGAAGTTATG ACCATGACCT CTTTACTATG ACTTGGGGGC CAACAATAGC TGCCTTGTCC
    TATGTGTTTG ATAAGAGCAT GGATGAAACG ATTATTCAGA AAGCAATATC TGGTTTTAGG
    AAATGTGCAA TGATATCTGC TCACTATGGT CTCAGTGATG TTTTTGACAA TCTCATAATT
    TCCTTGTGCA AGTTTACAAC ACTTAGCAGT GAAGCCGTTG AAAATCTTCC CACTGTTTTT
    GGAAGCAACC TAAAAGCTCA GATCGCTGCA AAGACTGTGT TCCACTTATC CCACAGACAT
    GGTGACATCC TTCGAGAGGG ATGGAAAAAT ATTATGGACT CCATGCTGCA GCTTTTTAGA
    GCTGAGCTGC TTCCCAAGGC TATGGTTGAG GTTGAAGATT TTGTAGACCC CAATGGAAAA
    ATATCTCTTC AACGTGAGGA AATCCCTGCC AACCGTGGGG AATCCACTGT ACTAAGTTTT
    GTCAGCTGGC TGACTCTGAG TGGGACAGAA CAGTCAAGTC TAAGAGGACC TTCTACTGAA
    AATCAAGAGG CCAAGAAACT AGCGCTGGAA TGTATCAAGT TATGTGATCC AGAGAAACTG
    ATCACAGAGA GCAAGTTTCT TCAACTGGAG TCTTTGCAAG AACTAATGAA GGCTCTGATA
    TCTGTGACAC CAGATGAAGA AACGTATGAT GAAGAAGATG CTGCATTCTG CCTAGAAATG
    TTAATGAGAA TTGTGCTAGA AAACAGAGAT CGTGTTGGCT GTGTTTGGCA GGCTGTACGA
    GATCATCTCT ATCGTCTATG TGTCCATGCT GTAGAGTTCT GCTTCCTGGT TGAAAGAGCA
    GTTGTAGGGC TGCTGCGCTT GGCAATACGC CTTTTACGCA GAGAGGAAAT TAGTGGTCAG
    GTTATCCTCT CCTTGAGAAT TCTTTTAATG ATGAAACCAA CTGTGTTGTC TAAAGTGAGT
    CGCCAAGTTG CTTTTGGTGT GCATGAGCTC TTGAAAACCA ATGCTGCTAA CATCCATTCC
    AGCAATGACT GGTACACACT ATTCTGCCTT TTGGAGTGCA TTGGGGCAGG AGTGAAGCCG
    CCCCCTGCCT TACAGGTGAC GACGAGAGCT GACAATGACA CAGGTGCGCA GTCTGACAGT
    GAAGTTTCCT CATACCATCC AAGTGAAGTG AATCTTGATC GTGGATACAC CTCAGACTCT
    GAGGTATATG CAGAACACAG CAAGCAGTCA AAAATGCACC GCTCTGTGAC TGATGTGGAT
    ATGATGAACA GTGGCTGGCT GATGGTTGGA AAGGAAGATG TTGACTCTTC TAAAGTTGCT
    ACAGTTATTA ACAAACCTGT TGCTAATGCC CCAGTGAATC AATACAGCCT AACCATTGGT
    TTGGATTTTG GCCCTCACGA TACCAAATCT CTCATGAAGA GCGTGGAGTC CCTTTCTTTT
    ATTGTTCGAG ATGCTGCACA TGTCACACCA GAAAACTTTG AACTCTGTGT TAAAACAATC
    CGTGTCTTTG TAGAGGCAAG TCTAAATGGA GGTGTGTGGT ACAAATCTCA GGAGAAAAGG
    GTAAAGAACC ACAAATATGA CAGTAAATCC AACCGCTTCA AAAAGAAAAC CAAGGAAAAA
    GAAAATTCTG TCCGCCGTAC ACGGGCTTCA AATCAGCGAC AATACCGATC TCACAGTGAT
    GATGAGGAAG ATGAAAGTGT ACCAGCCAGC TATCACACTG TGTCATTACA GCTTTTGGAC
    CTAATGCACA CACTTCACAC AAGAGCTGCC ACCATATACA GTTCTTGGGC AGAGGAACAG
    CGCAACCTAG AGACAGCTGC TGAGAAAATT ACAGCCGATT CTAAGACACT GTGGTCAAAC
    TGTTGGTGTC CTTTACTGCA AGGTATTGCT TGGCTCTGCT GTGATGCTCG GCGCCAGGTC
    AGAATGCAGG CTCTCACCTA CCTGCAAAGG GCACTTTTAG TCCATGACTT ACAGGCTCTT
    GATGCACTTG AATGGGAATC ATGCTTTAAT AAGGTGCTAT TTCCATTGCT GACCAAACTA
    TTGGAAAATA TTAGTCCAGC TGATGTTGGC GGGATGGAGG AGACCAGAAT GAGAGGCTCC
    ACTCTGCTCT CAAAGGTATT TCTACAGCAT CTGTCTCCTC TTCTTTCTCT GTCGACATTT
    GCTGCCTTGT GGCTTACTAT TCTTGATTTC ATGGACAAGT ACATGCATGC TGGCTCCAGT
    GACTTACTGC CTGAGGCAAT TCCAGAGTCA CTGAAAAATA TGCTTCTCGT AATGGACACT
    GCCGGAATAT TCCACAGTGC AGACTCGCGG ACGGGCTACT CTGATTTATG GGAGATCACG
    TGGGAAAGGA TAGATTGCTT TTTACCTCGT CTAAGGGAAG AACTGTTTAA ACAAACTGTG
    ATACAAGATC CACCCAATAT AGTTACAACA GAAACTCCTG TTGAGCCAAG ACCTCCCCCT
    CAATCAACTC AGTCAGAGAG ACCAGCGGAG CAGGGACCTT GTGACTCAGA GACTCAAAGT
    TTAGCAATCT CATCTGCTCC AGTAGTTGGA TCAAGTTCTT CCTGCACCCC AGTTAGAGTC
    AGCCCTGTAA CAGATATACC TCCACCTATA ACTCAGTCTC CAGTCATCCT GCATCCTCTG
    ACCTCCCCGC TGCAGGTGGG AGTGCCACCC ATGTCGCTGC CAATCATCCT CAACCCTGCA
    TTAATTGAAG CTACCTCTCC AGTTCCGCTC TTGTCCACTC CCCGACCTGC TGATGTATCA
    TCTGTCTCAG AGGTCAACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa62537
    Clone ID Related Accession (Same CDS sequence) XM_031906085.1
    Accession Version XM_031906085.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5655bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGA TCTCTCCATG TCTACTCTGC
    AAAATGGAGC TTAATAGTGG TGAACAGTCC AAAAACCTGA ACCAGTTAGA GAGGGTACTT
    CTTTTTAAGA GCCTGAAGTT GAAAATGAGA GCTGGAGGAA TGAGTGACTC CTCAAAATGG
    AAAAAGCAGA AGAAATCTCC TAGGCAGCCA CGTCATGTTA CCAAAGCTAT GGCGGCAGCA
    GAACCCTCCT CTCAGCAACC AGTAGTTAAT GGCACCACAA TTACTACTAA CCTCACTGGT
    GAAGTTCCTT TTATTGATGC TTCCTCTCCA GATGCTAATT TTCCCATTTC AGGGGTGAGT
    TCAGAGGCCA CGTCTGCTGT AGTCAGTCCT ACCACAGATA GTGGTGTAGA TATGTCCTCT
    CAGGCAACAT CTAAAGAAGA CCTTTCTGAA CTGGATCCTG CAAGTCCATC TCTTTCTGCC
    ATTCAGCAAT CAGGAGACAT TTGTCTTTCT GAACAGCAAA ACCAGGATGA TGATAAGATT
    GAAGACCTTA TGGAAAGGCA TACATATTCT GCTTCTGTGG AATCCATACC AGAAGTATTA
    GATGATAATG TGTCATTGGC TGATCAATCA GACTCCGCTT CAGTCCATGA TATGGATTAT
    GTAAATCCTA GAGGTGTGAG ATTTACCCAA TCTTCACAGA AAGAAGGAAC AGTCTTAGTA
    CCATATGGAC TGCCATGCAT TCGGGAATTA TTTCGTTTTC TTATTTCTTT AACCAACCCC
    CATGATCGAC ATAACTCCGA AGTGATGATA CACATGGGTT TACAGTTGAT CACAGTTGCT
    CTTGAGTCTG CACCTATTGA AAATTATGTT TCGCTTTTAG GCCTTGTAAA GGATGAACTT
    TGTCGACACT TATTTCAGCT ACTGAGCATT GAGAGACTGA ACCTATATGC AGCTTCCTTG
    CGAGCATGTT TTCTTTTGTT TGAGGGTATG AGGGAGCATT TGAAGTTCCA GCTAGAGATG
    TACATAAAGA AGCTCATGGA TATTATTACT GTGGAGAACC CTAAGATGCC TTATGAGATG
    AAAGAAATGG CTCTTGAAGC TATTGTGCAA TTGTGGAGAA TCCCGAGCTT TGTTACAGAA
    TTATACATTA ATTACGACTG TGATTATTAT TGCTCCAACC TTTTTGAAGA TCTCACTAAA
    CTGCTTTCCA AGAATGCCTT TCCAGTTTCT GGACAGCTGT ACACCACTCA TCTCCTTTCC
    CTGGAAGCTC TGCTAACTGT CATTGATAGC ACAGAATCAC ACTGCCAGGC TAAAAATCTA
    AGCAATGTGT CACAGGATAA GAAAGAAACT GGAAAACCAA GTGGAGATCT GTCAGAAGGG
    ACCAAAGATT CTGGAAATCT CAATGAACAC TCCATTGAAG TTAAGCCATT CACTGTTAGT
    GCAGAAAACA ATGGATCGAA GCCCCCCACA AGTGGCCACT TAATGGCAGA TAAAATGACT
    CTTGGGGTCC AAGAAACAGA GTACAGCACT GATGGAGGTG AGAAGAAGAC TTTAAAGAAG
    CCCCAACGCT TTTCCTGCTT ACTTCCGAAT GTTCAAGAGC TGAATGACAT TAAGACCAAA
    AAGAAGCTTC TTATAACTGG AACTGAGCAG TTCAACCAGA AACCAAAGAA AGGAGTGCAG
    TTTCTTCAGG AAAAAGACCT TTTGGCAACC CCCATGGATA ATGCTGAGGT AGCACAGTGG
    CTCAGAGAAA ATCCAAGGCT GGACAAGAAA ATGATTGGGG AGTTTGTGAG TGATCGCAAA
    AATCTGGACT TACTTGAAAG TTTTGTAGGA ACCTTCCATT TCCAAGGCCT GAGAGTTGAT
    GAAGCTTTAA GGCTTTACCT TGAAGCTTTT CGTTTGCCTG GCGAGGCACC TGTGATTCAG
    AGGTTACTGG AGGCTTTTAC AGAGCACTGG CGAAAATCCA ATGGAACTCC TTTTGCACAC
    AGTGATGCCT GCTTTGCTTT GGCTTATGCA GTTATCATGC TGAACACAGA CCAACACAAT
    CACAATGTCC GCAAGCAAAA TGTGCCAATG ACTTTAGAGG AATTCAGGAA AAATTTAAAG
    GGTGTTAATG GTGGAAAAGA TTTTGACCAA GATATGCTAG AGGATATTTA TCACGCAATC
    AAAAATGAAG AAATTGTAAT GCCTGAAGAG CAGACTGGCT TAGTAAAAGA GAATTATTTT
    TGGAATGTTT TGCTGCATCG GGGAGCCACT CCAGAAGGAA TGTTCTTGCA TGTGGATCCT
    GGAAGTTATG ACCATGACCT CTTTACTATG ACTTGGGGGC CAACAATAGC TGCCTTGTCC
    TATGTGTTTG ATAAGAGCAT GGATGAAACG ATTATTCAGA AAGCAATATC TGGTTTTAGG
    AAATGTGCAA TGATATCTGC TCACTATGGT CTCAGTGATG TTTTTGACAA TCTCATAATT
    TCCTTGTGCA AGTTTACAAC ACTTAGCAGT GAAGCCGTTG AAAATCTTCC CACTGTTTTT
    GGAAGCAACC TAAAAGCTCA GATCGCTGCA AAGACTGTGT TCCACTTATC CCACAGACAT
    GGTGACATCC TTCGAGAGGG ATGGAAAAAT ATTATGGACT CCATGCTGCA GCTTTTTAGA
    GCTGAGCTGC TTCCCAAGGC TATGGTTGAG GTTGAAGATT TTGTAGACCC CAATGGAAAA
    ATATCTCTTC AACGTGAGGA AATCCCTGCC AACCGTGGGG AATCCACTGT ACTAAGTTTT
    GTCAGCTGGC TGACTCTGAG TGGGACAGAA CAGTCAAGTC TAAGAGGACC TTCTACTGAA
    AATCAAGAGG CCAAGAAACT AGCGCTGGAA TGTATCAAGT TATGTGATCC AGAGAAACTG
    ATCACAGAGA GCAAGTTTCT TCAACTGGAG TCTTTGCAAG AACTAATGAA GGCTCTGATA
    TCTGTGACAC CAGATGAAGA AACGTATGAT GAAGAAGATG CTGCATTCTG CCTAGAAATG
    TTAATGAGAA TTGTGCTAGA AAACAGAGAT CGTGTTGGCT GTGTTTGGCA GGCTGTACGA
    GATCATCTCT ATCGTCTATG TGTCCATGCT GTAGAGTTCT GCTTCCTGGT TGAAAGAGCA
    GTTGTAGGGC TGCTGCGCTT GGCAATACGC CTTTTACGCA GAGAGGAAAT TAGTGGTCAG
    GTTATCCTCT CCTTGAGAAT TCTTTTAATG ATGAAACCAA CTGTGTTGTC TAAAGTGAGT
    CGCCAAGTTG CTTTTGGTGT GCATGAGCTC TTGAAAACCA ATGCTGCTAA CATCCATTCC
    AGCAATGACT GGTACACACT ATTCTGCCTT TTGGAGTGCA TTGGGGCAGG AGTGAAGCCG
    CCCCCTGCCT TACAGGTGAC GACGAGAGCT GACAATGACA CAGGTGCGCA GTCTGACAGT
    GAAGTTTCCT CATACCATCC AAGTGAAGTG AATCTTGATC GTGGATACAC CTCAGACTCT
    GAGGTATATG CAGAACACAG CAAGCAGTCA AAAATGCACC GCTCTGTGAC TGATGTGGAT
    ATGATGAACA GTGGCTGGCT GATGGTTGGA AAGGAAGATG TTGACTCTTC TAAAGTTGCT
    ACAGTTATTA ACAAACCTGT TGCTAATGCC CCAGTGAATC AATACAGCCT AACCATTGGT
    TTGGATTTTG GCCCTCACGA TACCAAATCT CTCATGAAGA GCGTGGAGTC CCTTTCTTTT
    ATTGTTCGAG ATGCTGCACA TGTCACACCA GAAAACTTTG AACTCTGTGT TAAAACAATC
    CGTGTCTTTG TAGAGGCAAG TCTAAATGGA GGGTACAAAT CTCAGGAGAA AAGGGTAAAG
    AACCACAAAT ATGACAGTAA ATCCAACCGC TTCAAAAAGA AAACCAAGGA AAAAGAAAAT
    TCTGTCCGCC GTACACGGGC TTCAAATCAG CGACAATACC GATCTCACAG TGATGATGAG
    GAAGATGAAA GTGTACCAGC CAGCTATCAC ACTGTGTCAT TACAGCTTTT GGACCTAATG
    CACACACTTC ACACAAGAGC TGCCACCATA TACAGTTCTT GGGCAGAGGA ACAGCGCAAC
    CTAGAGACAG CTGCTGAGAA AATTACAGCC GATTCTAAGA CACTGTGGTC AAACTGTTGG
    TGTCCTTTAC TGCAAGGTAT TGCTTGGCTC TGCTGTGATG CTCGGCGCCA GGTCAGAATG
    CAGGCTCTCA CCTACCTGCA AAGGGCACTT TTAGTCCATG ACTTACAGGC TCTTGATGCA
    CTTGAATGGG AATCATGCTT TAATAAGGTG CTATTTCCAT TGCTGACCAA ACTATTGGAA
    AATATTAGTC CAGCTGATGT TGGCGGGATG GAGGAGACCA GAATGAGAGG CTCCACTCTG
    CTCTCAAAGG TATTTCTACA GCATCTGTCT CCTCTTCTTT CTCTGTCGAC ATTTGCTGCC
    TTGTGGCTTA CTATTCTTGA TTTCATGGAC AAGTACATGC ATGCTGGCTC CAGTGACTTA
    CTGCCTGAGG CAATTCCAGA GTCACTGAAA AATATGCTTC TCGTAATGGA CACTGCCGGA
    ATATTCCACA GTGCAGACTC GCGGACGGGC TACTCTGATT TATGGGAGAT CACGTGGGAA
    AGGATAGATT GCTTTTTACC TCGTCTAAGG GAAGAACTGT TTAAACAAAC TGTGATACAA
    GATCCACCCA ATATAGTTAC AACAGAAACT CCTGTTGAGC CAAGACCTCC CCCTCAATCA
    ACTCAGTCAG AGAGACCAGC GGAGCAGGGA CCTTGTGACT CAGAGACTCA AAGTTTAGCA
    ATCTCATCTG CTCCAGTAGT TGGATCAAGT TCTTCCTGCA CCCCAGTTAG AGTCAGCCCT
    GTAACAGATA TACCTCCACC TATAACTCAG TCTCCAGTCA TCCTGCATCC TCTGACCTCC
    CCGCTGCAGG TGGGAGTGCC ACCCATGTCG CTGCCAATCA TCCTCAACCC TGCATTAATT
    GAAGCTACCT CTCCAGTTCC GCTCTTGTCC ACTCCCCGAC CTGCTGATGT ATCATCTGTC
    TCAGAGGTCA ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031761945.1
    CDS100..5754
    Translation

    Target ORF information:

    RefSeq Version XM_031906085.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031906085.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGA TCTCTCCATG TCTACTCTGC
    AAAATGGAGC TTAATAGTGG TGAACAGTCC AAAAACCTGA ACCAGTTAGA GAGGGTACTT
    CTTTTTAAGA GCCTGAAGTT GAAAATGAGA GCTGGAGGAA TGAGTGACTC CTCAAAATGG
    AAAAAGCAGA AGAAATCTCC TAGGCAGCCA CGTCATGTTA CCAAAGCTAT GGCGGCAGCA
    GAACCCTCCT CTCAGCAACC AGTAGTTAAT GGCACCACAA TTACTACTAA CCTCACTGGT
    GAAGTTCCTT TTATTGATGC TTCCTCTCCA GATGCTAATT TTCCCATTTC AGGGGTGAGT
    TCAGAGGCCA CGTCTGCTGT AGTCAGTCCT ACCACAGATA GTGGTGTAGA TATGTCCTCT
    CAGGCAACAT CTAAAGAAGA CCTTTCTGAA CTGGATCCTG CAAGTCCATC TCTTTCTGCC
    ATTCAGCAAT CAGGAGACAT TTGTCTTTCT GAACAGCAAA ACCAGGATGA TGATAAGATT
    GAAGACCTTA TGGAAAGGCA TACATATTCT GCTTCTGTGG AATCCATACC AGAAGTATTA
    GATGATAATG TGTCATTGGC TGATCAATCA GACTCCGCTT CAGTCCATGA TATGGATTAT
    GTAAATCCTA GAGGTGTGAG ATTTACCCAA TCTTCACAGA AAGAAGGAAC AGTCTTAGTA
    CCATATGGAC TGCCATGCAT TCGGGAATTA TTTCGTTTTC TTATTTCTTT AACCAACCCC
    CATGATCGAC ATAACTCCGA AGTGATGATA CACATGGGTT TACAGTTGAT CACAGTTGCT
    CTTGAGTCTG CACCTATTGA AAATTATGTT TCGCTTTTAG GCCTTGTAAA GGATGAACTT
    TGTCGACACT TATTTCAGCT ACTGAGCATT GAGAGACTGA ACCTATATGC AGCTTCCTTG
    CGAGCATGTT TTCTTTTGTT TGAGGGTATG AGGGAGCATT TGAAGTTCCA GCTAGAGATG
    TACATAAAGA AGCTCATGGA TATTATTACT GTGGAGAACC CTAAGATGCC TTATGAGATG
    AAAGAAATGG CTCTTGAAGC TATTGTGCAA TTGTGGAGAA TCCCGAGCTT TGTTACAGAA
    TTATACATTA ATTACGACTG TGATTATTAT TGCTCCAACC TTTTTGAAGA TCTCACTAAA
    CTGCTTTCCA AGAATGCCTT TCCAGTTTCT GGACAGCTGT ACACCACTCA TCTCCTTTCC
    CTGGAAGCTC TGCTAACTGT CATTGATAGC ACAGAATCAC ACTGCCAGGC TAAAAATCTA
    AGCAATGTGT CACAGGATAA GAAAGAAACT GGAAAACCAA GTGGAGATCT GTCAGAAGGG
    ACCAAAGATT CTGGAAATCT CAATGAACAC TCCATTGAAG TTAAGCCATT CACTGTTAGT
    GCAGAAAACA ATGGATCGAA GCCCCCCACA AGTGGCCACT TAATGGCAGA TAAAATGACT
    CTTGGGGTCC AAGAAACAGA GTACAGCACT GATGGAGGTG AGAAGAAGAC TTTAAAGAAG
    CCCCAACGCT TTTCCTGCTT ACTTCCGAAT GTTCAAGAGC TGAATGACAT TAAGACCAAA
    AAGAAGCTTC TTATAACTGG AACTGAGCAG TTCAACCAGA AACCAAAGAA AGGAGTGCAG
    TTTCTTCAGG AAAAAGACCT TTTGGCAACC CCCATGGATA ATGCTGAGGT AGCACAGTGG
    CTCAGAGAAA ATCCAAGGCT GGACAAGAAA ATGATTGGGG AGTTTGTGAG TGATCGCAAA
    AATCTGGACT TACTTGAAAG TTTTGTAGGA ACCTTCCATT TCCAAGGCCT GAGAGTTGAT
    GAAGCTTTAA GGCTTTACCT TGAAGCTTTT CGTTTGCCTG GCGAGGCACC TGTGATTCAG
    AGGTTACTGG AGGCTTTTAC AGAGCACTGG CGAAAATCCA ATGGAACTCC TTTTGCACAC
    AGTGATGCCT GCTTTGCTTT GGCTTATGCA GTTATCATGC TGAACACAGA CCAACACAAT
    CACAATGTCC GCAAGCAAAA TGTGCCAATG ACTTTAGAGG AATTCAGGAA AAATTTAAAG
    GGTGTTAATG GTGGAAAAGA TTTTGACCAA GATATGCTAG AGGATATTTA TCACGCAATC
    AAAAATGAAG AAATTGTAAT GCCTGAAGAG CAGACTGGCT TAGTAAAAGA GAATTATTTT
    TGGAATGTTT TGCTGCATCG GGGAGCCACT CCAGAAGGAA TGTTCTTGCA TGTGGATCCT
    GGAAGTTATG ACCATGACCT CTTTACTATG ACTTGGGGGC CAACAATAGC TGCCTTGTCC
    TATGTGTTTG ATAAGAGCAT GGATGAAACG ATTATTCAGA AAGCAATATC TGGTTTTAGG
    AAATGTGCAA TGATATCTGC TCACTATGGT CTCAGTGATG TTTTTGACAA TCTCATAATT
    TCCTTGTGCA AGTTTACAAC ACTTAGCAGT GAAGCCGTTG AAAATCTTCC CACTGTTTTT
    GGAAGCAACC TAAAAGCTCA GATCGCTGCA AAGACTGTGT TCCACTTATC CCACAGACAT
    GGTGACATCC TTCGAGAGGG ATGGAAAAAT ATTATGGACT CCATGCTGCA GCTTTTTAGA
    GCTGAGCTGC TTCCCAAGGC TATGGTTGAG GTTGAAGATT TTGTAGACCC CAATGGAAAA
    ATATCTCTTC AACGTGAGGA AATCCCTGCC AACCGTGGGG AATCCACTGT ACTAAGTTTT
    GTCAGCTGGC TGACTCTGAG TGGGACAGAA CAGTCAAGTC TAAGAGGACC TTCTACTGAA
    AATCAAGAGG CCAAGAAACT AGCGCTGGAA TGTATCAAGT TATGTGATCC AGAGAAACTG
    ATCACAGAGA GCAAGTTTCT TCAACTGGAG TCTTTGCAAG AACTAATGAA GGCTCTGATA
    TCTGTGACAC CAGATGAAGA AACGTATGAT GAAGAAGATG CTGCATTCTG CCTAGAAATG
    TTAATGAGAA TTGTGCTAGA AAACAGAGAT CGTGTTGGCT GTGTTTGGCA GGCTGTACGA
    GATCATCTCT ATCGTCTATG TGTCCATGCT GTAGAGTTCT GCTTCCTGGT TGAAAGAGCA
    GTTGTAGGGC TGCTGCGCTT GGCAATACGC CTTTTACGCA GAGAGGAAAT TAGTGGTCAG
    GTTATCCTCT CCTTGAGAAT TCTTTTAATG ATGAAACCAA CTGTGTTGTC TAAAGTGAGT
    CGCCAAGTTG CTTTTGGTGT GCATGAGCTC TTGAAAACCA ATGCTGCTAA CATCCATTCC
    AGCAATGACT GGTACACACT ATTCTGCCTT TTGGAGTGCA TTGGGGCAGG AGTGAAGCCG
    CCCCCTGCCT TACAGGTGAC GACGAGAGCT GACAATGACA CAGGTGCGCA GTCTGACAGT
    GAAGTTTCCT CATACCATCC AAGTGAAGTG AATCTTGATC GTGGATACAC CTCAGACTCT
    GAGGTATATG CAGAACACAG CAAGCAGTCA AAAATGCACC GCTCTGTGAC TGATGTGGAT
    ATGATGAACA GTGGCTGGCT GATGGTTGGA AAGGAAGATG TTGACTCTTC TAAAGTTGCT
    ACAGTTATTA ACAAACCTGT TGCTAATGCC CCAGTGAATC AATACAGCCT AACCATTGGT
    TTGGATTTTG GCCCTCACGA TACCAAATCT CTCATGAAGA GCGTGGAGTC CCTTTCTTTT
    ATTGTTCGAG ATGCTGCACA TGTCACACCA GAAAACTTTG AACTCTGTGT TAAAACAATC
    CGTGTCTTTG TAGAGGCAAG TCTAAATGGA GGGTACAAAT CTCAGGAGAA AAGGGTAAAG
    AACCACAAAT ATGACAGTAA ATCCAACCGC TTCAAAAAGA AAACCAAGGA AAAAGAAAAT
    TCTGTCCGCC GTACACGGGC TTCAAATCAG CGACAATACC GATCTCACAG TGATGATGAG
    GAAGATGAAA GTGTACCAGC CAGCTATCAC ACTGTGTCAT TACAGCTTTT GGACCTAATG
    CACACACTTC ACACAAGAGC TGCCACCATA TACAGTTCTT GGGCAGAGGA ACAGCGCAAC
    CTAGAGACAG CTGCTGAGAA AATTACAGCC GATTCTAAGA CACTGTGGTC AAACTGTTGG
    TGTCCTTTAC TGCAAGGTAT TGCTTGGCTC TGCTGTGATG CTCGGCGCCA GGTCAGAATG
    CAGGCTCTCA CCTACCTGCA AAGGGCACTT TTAGTCCATG ACTTACAGGC TCTTGATGCA
    CTTGAATGGG AATCATGCTT TAATAAGGTG CTATTTCCAT TGCTGACCAA ACTATTGGAA
    AATATTAGTC CAGCTGATGT TGGCGGGATG GAGGAGACCA GAATGAGAGG CTCCACTCTG
    CTCTCAAAGG TATTTCTACA GCATCTGTCT CCTCTTCTTT CTCTGTCGAC ATTTGCTGCC
    TTGTGGCTTA CTATTCTTGA TTTCATGGAC AAGTACATGC ATGCTGGCTC CAGTGACTTA
    CTGCCTGAGG CAATTCCAGA GTCACTGAAA AATATGCTTC TCGTAATGGA CACTGCCGGA
    ATATTCCACA GTGCAGACTC GCGGACGGGC TACTCTGATT TATGGGAGAT CACGTGGGAA
    AGGATAGATT GCTTTTTACC TCGTCTAAGG GAAGAACTGT TTAAACAAAC TGTGATACAA
    GATCCACCCA ATATAGTTAC AACAGAAACT CCTGTTGAGC CAAGACCTCC CCCTCAATCA
    ACTCAGTCAG AGAGACCAGC GGAGCAGGGA CCTTGTGACT CAGAGACTCA AAGTTTAGCA
    ATCTCATCTG CTCCAGTAGT TGGATCAAGT TCTTCCTGCA CCCCAGTTAG AGTCAGCCCT
    GTAACAGATA TACCTCCACC TATAACTCAG TCTCCAGTCA TCCTGCATCC TCTGACCTCC
    CCGCTGCAGG TGGGAGTGCC ACCCATGTCG CTGCCAATCA TCCTCAACCC TGCATTAATT
    GAAGCTACCT CTCCAGTTCC GCTCTTGTCC ACTCCCCGAC CTGCTGATGT ATCATCTGTC
    TCAGAGGTCA ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa62538
    Clone ID Related Accession (Same CDS sequence) XM_012966851.3 , XM_012966852.3
    Accession Version XM_012966851.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5574bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966851.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGT TGAAAATGAG AGCTGGAGGA
    ATGAGTGACT CCTCAAAATG GAAAAAGCAG AAGAAATCTC CTAGGCAGCC ACGTCATGTT
    ACCAAAGCTA TGGCGGCAGC AGAACCCTCC TCTCAGCAAC CAGTAGTTAA TGGCACCACA
    ATTACTACTA ACCTCACTGG TGAAGTTCCT TTTATTGATG CTTCCTCTCC AGATGCTAAT
    TTTCCCATTT CAGGGGTGAG TTCAGAGGCC ACGTCTGCTG TAGTCAGTCC TACCACAGAT
    AGTGGTGTAG ATATGTCCTC TCAGGCAACA TCTAAAGAAG ACCTTTCTGA ACTGGATCCT
    GCAAGTCCAT CTCTTTCTGC CATTCAGCAA TCAGGAGACA TTTGTCTTTC TGAACAGCAA
    AACCAGGATG ATGATAAGAT TGAAGACCTT ATGGAAAGGC ATACATATTC TGCTTCTGTG
    GAATCCATAC CAGAAGTATT AGATGATAAT GTGTCATTGG CTGATCAATC AGACTCCGCT
    TCAGTCCATG ATATGGATTA TGTAAATCCT AGAGGTGTGA GATTTACCCA ATCTTCACAG
    AAAGAAGGAA CAGTCTTAGT ACCATATGGA CTGCCATGCA TTCGGGAATT ATTTCGTTTT
    CTTATTTCTT TAACCAACCC CCATGATCGA CATAACTCCG AAGTGATGAT ACACATGGGT
    TTACAGTTGA TCACAGTTGC TCTTGAGTCT GCACCTATTG AAAATTATGT TTCGCTTTTA
    GGCCTTGTAA AGGATGAACT TTGTCGACAC TTATTTCAGC TACTGAGCAT TGAGAGACTG
    AACCTATATG CAGCTTCCTT GCGAGCATGT TTTCTTTTGT TTGAGGGTAT GAGGGAGCAT
    TTGAAGTTCC AGCTAGAGAT GTACATAAAG AAGCTCATGG ATATTATTAC TGTGGAGAAC
    CCTAAGATGC CTTATGAGAT GAAAGAAATG GCTCTTGAAG CTATTGTGCA ATTGTGGAGA
    ATCCCGAGCT TTGTTACAGA ATTATACATT AATTACGACT GTGATTATTA TTGCTCCAAC
    CTTTTTGAAG ATCTCACTAA ACTGCTTTCC AAGAATGCCT TTCCAGTTTC TGGACAGCTG
    TACACCACTC ATCTCCTTTC CCTGGAAGCT CTGCTAACTG TCATTGATAG CACAGAATCA
    CACTGCCAGG CTAAAAATCT AAGCAATGTG TCACAGGATA AGAAAGAAAC TGGAAAACCA
    AGTGGAGATC TGTCAGAAGG GACCAAAGAT TCTGGAAATC TCAATGAACA CTCCATTGAA
    GTTAAGCCAT TCACTGTTAG TGCAGAAAAC AATGGATCGA AGCCCCCCAC AAGTGGCCAC
    TTAATGGCAG ATAAAATGAC TCTTGGGGTC CAAGAAACAG AGTACAGCAC TGATGGAGGT
    GAGAAGAAGA CTTTAAAGAA GCCCCAACGC TTTTCCTGCT TACTTCCGAA TGTTCAAGAG
    CTGAATGACA TTAAGACCAA AAAGAAGCTT CTTATAACTG GAACTGAGCA GTTCAACCAG
    AAACCAAAGA AAGGAGTGCA GTTTCTTCAG GAAAAAGACC TTTTGGCAAC CCCCATGGAT
    AATGCTGAGG TAGCACAGTG GCTCAGAGAA AATCCAAGGC TGGACAAGAA AATGATTGGG
    GAGTTTGTGA GTGATCGCAA AAATCTGGAC TTACTTGAAA GTTTTGTAGG AACCTTCCAT
    TTCCAAGGCC TGAGAGTTGA TGAAGCTTTA AGGCTTTACC TTGAAGCTTT TCGTTTGCCT
    GGCGAGGCAC CTGTGATTCA GAGGTTACTG GAGGCTTTTA CAGAGCACTG GCGAAAATCC
    AATGGAACTC CTTTTGCACA CAGTGATGCC TGCTTTGCTT TGGCTTATGC AGTTATCATG
    CTGAACACAG ACCAACACAA TCACAATGTC CGCAAGCAAA ATGTGCCAAT GACTTTAGAG
    GAATTCAGGA AAAATTTAAA GGGTGTTAAT GGTGGAAAAG ATTTTGACCA AGATATGCTA
    GAGGATATTT ATCACGCAAT CAAAAATGAA GAAATTGTAA TGCCTGAAGA GCAGACTGGC
    TTAGTAAAAG AGAATTATTT TTGGAATGTT TTGCTGCATC GGGGAGCCAC TCCAGAAGGA
    ATGTTCTTGC ATGTGGATCC TGGAAGTTAT GACCATGACC TCTTTACTAT GACTTGGGGG
    CCAACAATAG CTGCCTTGTC CTATGTGTTT GATAAGAGCA TGGATGAAAC GATTATTCAG
    AAAGCAATAT CTGGTTTTAG GAAATGTGCA ATGATATCTG CTCACTATGG TCTCAGTGAT
    GTTTTTGACA ATCTCATAAT TTCCTTGTGC AAGTTTACAA CACTTAGCAG TGAAGCCGTT
    GAAAATCTTC CCACTGTTTT TGGAAGCAAC CTAAAAGCTC AGATCGCTGC AAAGACTGTG
    TTCCACTTAT CCCACAGACA TGGTGACATC CTTCGAGAGG GATGGAAAAA TATTATGGAC
    TCCATGCTGC AGCTTTTTAG AGCTGAGCTG CTTCCCAAGG CTATGGTTGA GGTTGAAGAT
    TTTGTAGACC CCAATGGAAA AATATCTCTT CAACGTGAGG AAATCCCTGC CAACCGTGGG
    GAATCCACTG TACTAAGTTT TGTCAGCTGG CTGACTCTGA GTGGGACAGA ACAGTCAAGT
    CTAAGAGGAC CTTCTACTGA AAATCAAGAG GCCAAGAAAC TAGCGCTGGA ATGTATCAAG
    TTATGTGATC CAGAGAAACT GATCACAGAG AGCAAGTTTC TTCAACTGGA GTCTTTGCAA
    GAACTAATGA AGGCTCTGAT ATCTGTGACA CCAGATGAAG AAACGTATGA TGAAGAAGAT
    GCTGCATTCT GCCTAGAAAT GTTAATGAGA ATTGTGCTAG AAAACAGAGA TCGTGTTGGC
    TGTGTTTGGC AGGCTGTACG AGATCATCTC TATCGTCTAT GTGTCCATGC TGTAGAGTTC
    TGCTTCCTGG TTGAAAGAGC AGTTGTAGGG CTGCTGCGCT TGGCAATACG CCTTTTACGC
    AGAGAGGAAA TTAGTGGTCA GGTTATCCTC TCCTTGAGAA TTCTTTTAAT GATGAAACCA
    ACTGTGTTGT CTAAAGTGAG TCGCCAAGTT GCTTTTGGTG TGCATGAGCT CTTGAAAACC
    AATGCTGCTA ACATCCATTC CAGCAATGAC TGGTACACAC TATTCTGCCT TTTGGAGTGC
    ATTGGGGCAG GAGTGAAGCC GCCCCCTGCC TTACAGGTGA CGACGAGAGC TGACAATGAC
    ACAGGTGCGC AGTCTGACAG TGAAGTTTCC TCATACCATC CAAGTGAAGT GAATCTTGAT
    CGTGGATACA CCTCAGACTC TGAGGTATAT GCAGAACACA GCAAGCAGTC AAAAATGCAC
    CGCTCTGTGA CTGATGTGGA TATGATGAAC AGTGGCTGGC TGATGGTTGG AAAGGAAGAT
    GTTGACTCTT CTAAAGTTGC TACAGTTATT AACAAACCTG TTGCTAATGC CCCAGTGAAT
    CAATACAGCC TAACCATTGG TTTGGATTTT GGCCCTCACG ATACCAAATC TCTCATGAAG
    AGCGTGGAGT CCCTTTCTTT TATTGTTCGA GATGCTGCAC ATGTCACACC AGAAAACTTT
    GAACTCTGTG TTAAAACAAT CCGTGTCTTT GTAGAGGCAA GTCTAAATGG AGGTGTGTGG
    TACAAATCTC AGGAGAAAAG GGTAAAGAAC CACAAATATG ACAGTAAATC CAACCGCTTC
    AAAAAGAAAA CCAAGGAAAA AGAAAATTCT GTCCGCCGTA CACGGGCTTC AAATCAGCGA
    CAATACCGAT CTCACAGTGA TGATGAGGAA GATGAAAGTG TACCAGCCAG CTATCACACT
    GTGTCATTAC AGGTTAGTCA TGATCTTTTG GACCTAATGC ACACACTTCA CACAAGAGCT
    GCCACCATAT ACAGTTCTTG GGCAGAGGAA CAGCGCAACC TAGAGACAGC TGCTGAGAAA
    ATTACAGCCG ATTCTAAGAC ACTGTGGTCA AACTGTTGGT GTCCTTTACT GCAAGGTATT
    GCTTGGCTCT GCTGTGATGC TCGGCGCCAG GTCAGAATGC AGGCTCTCAC CTACCTGCAA
    AGGGCACTTT TAGTCCATGA CTTACAGGCT CTTGATGCAC TTGAATGGGA ATCATGCTTT
    AATAAGGTGC TATTTCCATT GCTGACCAAA CTATTGGAAA ATATTAGTCC AGCTGATGTT
    GGCGGGATGG AGGAGACCAG AATGAGAGGC TCCACTCTGC TCTCAAAGGT ATTTCTACAG
    CATCTGTCTC CTCTTCTTTC TCTGTCGACA TTTGCTGCCT TGTGGCTTAC TATTCTTGAT
    TTCATGGACA AGTACATGCA TGCTGGCTCC AGTGACTTAC TGCCTGAGGC AATTCCAGAG
    TCACTGAAAA ATATGCTTCT CGTAATGGAC ACTGCCGGAA TATTCCACAG TGCAGACTCG
    CGGACGGGCT ACTCTGATTT ATGGGAGATC ACGTGGGAAA GGATAGATTG CTTTTTACCT
    CGTCTAAGGG AAGAACTGTT TAAACAAACT GTGATACAAG ATCCACCCAA TATAGTTACA
    ACAGAAACTC CTGTTGAGCC AAGACCTCCC CCTCAATCAA CTCAGTCAGA GAGACCAGCG
    GAGCAGGGAC CTTGTGACTC AGAGACTCAA AGTTTAGCAA TCTCATCTGC TCCAGTAGTT
    GGATCAAGTT CTTCCTGCAC CCCAGTTAGA GTCAGCCCTG TAACAGATAT ACCTCCACCT
    ATAACTCAGT CTCCAGTCAT CCTGCATCCT CTGACCTCCC CGCTGCAGGT GGGAGTGCCA
    CCCATGTCGC TGCCAATCAT CCTCAACCCT GCATTAATTG AAGCTACCTC TCCAGTTCCG
    CTCTTGTCCA CTCCCCGACC TGCTGATGTA TCATCTGTCT CAGAGGTCAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012822305.1
    CDS262..5835
    Translation

    Target ORF information:

    RefSeq Version XM_012966851.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966851.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGT TGAAAATGAG AGCTGGAGGA
    ATGAGTGACT CCTCAAAATG GAAAAAGCAG AAGAAATCTC CTAGGCAGCC ACGTCATGTT
    ACCAAAGCTA TGGCGGCAGC AGAACCCTCC TCTCAGCAAC CAGTAGTTAA TGGCACCACA
    ATTACTACTA ACCTCACTGG TGAAGTTCCT TTTATTGATG CTTCCTCTCC AGATGCTAAT
    TTTCCCATTT CAGGGGTGAG TTCAGAGGCC ACGTCTGCTG TAGTCAGTCC TACCACAGAT
    AGTGGTGTAG ATATGTCCTC TCAGGCAACA TCTAAAGAAG ACCTTTCTGA ACTGGATCCT
    GCAAGTCCAT CTCTTTCTGC CATTCAGCAA TCAGGAGACA TTTGTCTTTC TGAACAGCAA
    AACCAGGATG ATGATAAGAT TGAAGACCTT ATGGAAAGGC ATACATATTC TGCTTCTGTG
    GAATCCATAC CAGAAGTATT AGATGATAAT GTGTCATTGG CTGATCAATC AGACTCCGCT
    TCAGTCCATG ATATGGATTA TGTAAATCCT AGAGGTGTGA GATTTACCCA ATCTTCACAG
    AAAGAAGGAA CAGTCTTAGT ACCATATGGA CTGCCATGCA TTCGGGAATT ATTTCGTTTT
    CTTATTTCTT TAACCAACCC CCATGATCGA CATAACTCCG AAGTGATGAT ACACATGGGT
    TTACAGTTGA TCACAGTTGC TCTTGAGTCT GCACCTATTG AAAATTATGT TTCGCTTTTA
    GGCCTTGTAA AGGATGAACT TTGTCGACAC TTATTTCAGC TACTGAGCAT TGAGAGACTG
    AACCTATATG CAGCTTCCTT GCGAGCATGT TTTCTTTTGT TTGAGGGTAT GAGGGAGCAT
    TTGAAGTTCC AGCTAGAGAT GTACATAAAG AAGCTCATGG ATATTATTAC TGTGGAGAAC
    CCTAAGATGC CTTATGAGAT GAAAGAAATG GCTCTTGAAG CTATTGTGCA ATTGTGGAGA
    ATCCCGAGCT TTGTTACAGA ATTATACATT AATTACGACT GTGATTATTA TTGCTCCAAC
    CTTTTTGAAG ATCTCACTAA ACTGCTTTCC AAGAATGCCT TTCCAGTTTC TGGACAGCTG
    TACACCACTC ATCTCCTTTC CCTGGAAGCT CTGCTAACTG TCATTGATAG CACAGAATCA
    CACTGCCAGG CTAAAAATCT AAGCAATGTG TCACAGGATA AGAAAGAAAC TGGAAAACCA
    AGTGGAGATC TGTCAGAAGG GACCAAAGAT TCTGGAAATC TCAATGAACA CTCCATTGAA
    GTTAAGCCAT TCACTGTTAG TGCAGAAAAC AATGGATCGA AGCCCCCCAC AAGTGGCCAC
    TTAATGGCAG ATAAAATGAC TCTTGGGGTC CAAGAAACAG AGTACAGCAC TGATGGAGGT
    GAGAAGAAGA CTTTAAAGAA GCCCCAACGC TTTTCCTGCT TACTTCCGAA TGTTCAAGAG
    CTGAATGACA TTAAGACCAA AAAGAAGCTT CTTATAACTG GAACTGAGCA GTTCAACCAG
    AAACCAAAGA AAGGAGTGCA GTTTCTTCAG GAAAAAGACC TTTTGGCAAC CCCCATGGAT
    AATGCTGAGG TAGCACAGTG GCTCAGAGAA AATCCAAGGC TGGACAAGAA AATGATTGGG
    GAGTTTGTGA GTGATCGCAA AAATCTGGAC TTACTTGAAA GTTTTGTAGG AACCTTCCAT
    TTCCAAGGCC TGAGAGTTGA TGAAGCTTTA AGGCTTTACC TTGAAGCTTT TCGTTTGCCT
    GGCGAGGCAC CTGTGATTCA GAGGTTACTG GAGGCTTTTA CAGAGCACTG GCGAAAATCC
    AATGGAACTC CTTTTGCACA CAGTGATGCC TGCTTTGCTT TGGCTTATGC AGTTATCATG
    CTGAACACAG ACCAACACAA TCACAATGTC CGCAAGCAAA ATGTGCCAAT GACTTTAGAG
    GAATTCAGGA AAAATTTAAA GGGTGTTAAT GGTGGAAAAG ATTTTGACCA AGATATGCTA
    GAGGATATTT ATCACGCAAT CAAAAATGAA GAAATTGTAA TGCCTGAAGA GCAGACTGGC
    TTAGTAAAAG AGAATTATTT TTGGAATGTT TTGCTGCATC GGGGAGCCAC TCCAGAAGGA
    ATGTTCTTGC ATGTGGATCC TGGAAGTTAT GACCATGACC TCTTTACTAT GACTTGGGGG
    CCAACAATAG CTGCCTTGTC CTATGTGTTT GATAAGAGCA TGGATGAAAC GATTATTCAG
    AAAGCAATAT CTGGTTTTAG GAAATGTGCA ATGATATCTG CTCACTATGG TCTCAGTGAT
    GTTTTTGACA ATCTCATAAT TTCCTTGTGC AAGTTTACAA CACTTAGCAG TGAAGCCGTT
    GAAAATCTTC CCACTGTTTT TGGAAGCAAC CTAAAAGCTC AGATCGCTGC AAAGACTGTG
    TTCCACTTAT CCCACAGACA TGGTGACATC CTTCGAGAGG GATGGAAAAA TATTATGGAC
    TCCATGCTGC AGCTTTTTAG AGCTGAGCTG CTTCCCAAGG CTATGGTTGA GGTTGAAGAT
    TTTGTAGACC CCAATGGAAA AATATCTCTT CAACGTGAGG AAATCCCTGC CAACCGTGGG
    GAATCCACTG TACTAAGTTT TGTCAGCTGG CTGACTCTGA GTGGGACAGA ACAGTCAAGT
    CTAAGAGGAC CTTCTACTGA AAATCAAGAG GCCAAGAAAC TAGCGCTGGA ATGTATCAAG
    TTATGTGATC CAGAGAAACT GATCACAGAG AGCAAGTTTC TTCAACTGGA GTCTTTGCAA
    GAACTAATGA AGGCTCTGAT ATCTGTGACA CCAGATGAAG AAACGTATGA TGAAGAAGAT
    GCTGCATTCT GCCTAGAAAT GTTAATGAGA ATTGTGCTAG AAAACAGAGA TCGTGTTGGC
    TGTGTTTGGC AGGCTGTACG AGATCATCTC TATCGTCTAT GTGTCCATGC TGTAGAGTTC
    TGCTTCCTGG TTGAAAGAGC AGTTGTAGGG CTGCTGCGCT TGGCAATACG CCTTTTACGC
    AGAGAGGAAA TTAGTGGTCA GGTTATCCTC TCCTTGAGAA TTCTTTTAAT GATGAAACCA
    ACTGTGTTGT CTAAAGTGAG TCGCCAAGTT GCTTTTGGTG TGCATGAGCT CTTGAAAACC
    AATGCTGCTA ACATCCATTC CAGCAATGAC TGGTACACAC TATTCTGCCT TTTGGAGTGC
    ATTGGGGCAG GAGTGAAGCC GCCCCCTGCC TTACAGGTGA CGACGAGAGC TGACAATGAC
    ACAGGTGCGC AGTCTGACAG TGAAGTTTCC TCATACCATC CAAGTGAAGT GAATCTTGAT
    CGTGGATACA CCTCAGACTC TGAGGTATAT GCAGAACACA GCAAGCAGTC AAAAATGCAC
    CGCTCTGTGA CTGATGTGGA TATGATGAAC AGTGGCTGGC TGATGGTTGG AAAGGAAGAT
    GTTGACTCTT CTAAAGTTGC TACAGTTATT AACAAACCTG TTGCTAATGC CCCAGTGAAT
    CAATACAGCC TAACCATTGG TTTGGATTTT GGCCCTCACG ATACCAAATC TCTCATGAAG
    AGCGTGGAGT CCCTTTCTTT TATTGTTCGA GATGCTGCAC ATGTCACACC AGAAAACTTT
    GAACTCTGTG TTAAAACAAT CCGTGTCTTT GTAGAGGCAA GTCTAAATGG AGGTGTGTGG
    TACAAATCTC AGGAGAAAAG GGTAAAGAAC CACAAATATG ACAGTAAATC CAACCGCTTC
    AAAAAGAAAA CCAAGGAAAA AGAAAATTCT GTCCGCCGTA CACGGGCTTC AAATCAGCGA
    CAATACCGAT CTCACAGTGA TGATGAGGAA GATGAAAGTG TACCAGCCAG CTATCACACT
    GTGTCATTAC AGGTTAGTCA TGATCTTTTG GACCTAATGC ACACACTTCA CACAAGAGCT
    GCCACCATAT ACAGTTCTTG GGCAGAGGAA CAGCGCAACC TAGAGACAGC TGCTGAGAAA
    ATTACAGCCG ATTCTAAGAC ACTGTGGTCA AACTGTTGGT GTCCTTTACT GCAAGGTATT
    GCTTGGCTCT GCTGTGATGC TCGGCGCCAG GTCAGAATGC AGGCTCTCAC CTACCTGCAA
    AGGGCACTTT TAGTCCATGA CTTACAGGCT CTTGATGCAC TTGAATGGGA ATCATGCTTT
    AATAAGGTGC TATTTCCATT GCTGACCAAA CTATTGGAAA ATATTAGTCC AGCTGATGTT
    GGCGGGATGG AGGAGACCAG AATGAGAGGC TCCACTCTGC TCTCAAAGGT ATTTCTACAG
    CATCTGTCTC CTCTTCTTTC TCTGTCGACA TTTGCTGCCT TGTGGCTTAC TATTCTTGAT
    TTCATGGACA AGTACATGCA TGCTGGCTCC AGTGACTTAC TGCCTGAGGC AATTCCAGAG
    TCACTGAAAA ATATGCTTCT CGTAATGGAC ACTGCCGGAA TATTCCACAG TGCAGACTCG
    CGGACGGGCT ACTCTGATTT ATGGGAGATC ACGTGGGAAA GGATAGATTG CTTTTTACCT
    CGTCTAAGGG AAGAACTGTT TAAACAAACT GTGATACAAG ATCCACCCAA TATAGTTACA
    ACAGAAACTC CTGTTGAGCC AAGACCTCCC CCTCAATCAA CTCAGTCAGA GAGACCAGCG
    GAGCAGGGAC CTTGTGACTC AGAGACTCAA AGTTTAGCAA TCTCATCTGC TCCAGTAGTT
    GGATCAAGTT CTTCCTGCAC CCCAGTTAGA GTCAGCCCTG TAACAGATAT ACCTCCACCT
    ATAACTCAGT CTCCAGTCAT CCTGCATCCT CTGACCTCCC CGCTGCAGGT GGGAGTGCCA
    CCCATGTCGC TGCCAATCAT CCTCAACCCT GCATTAATTG AAGCTACCTC TCCAGTTCCG
    CTCTTGTCCA CTCCCCGACC TGCTGATGTA TCATCTGTCT CAGAGGTCAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa62538
    Clone ID Related Accession (Same CDS sequence) XM_012966851.3 , XM_012966852.3
    Accession Version XM_012966852.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5574bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966852.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGT TGAAAATGAG AGCTGGAGGA
    ATGAGTGACT CCTCAAAATG GAAAAAGCAG AAGAAATCTC CTAGGCAGCC ACGTCATGTT
    ACCAAAGCTA TGGCGGCAGC AGAACCCTCC TCTCAGCAAC CAGTAGTTAA TGGCACCACA
    ATTACTACTA ACCTCACTGG TGAAGTTCCT TTTATTGATG CTTCCTCTCC AGATGCTAAT
    TTTCCCATTT CAGGGGTGAG TTCAGAGGCC ACGTCTGCTG TAGTCAGTCC TACCACAGAT
    AGTGGTGTAG ATATGTCCTC TCAGGCAACA TCTAAAGAAG ACCTTTCTGA ACTGGATCCT
    GCAAGTCCAT CTCTTTCTGC CATTCAGCAA TCAGGAGACA TTTGTCTTTC TGAACAGCAA
    AACCAGGATG ATGATAAGAT TGAAGACCTT ATGGAAAGGC ATACATATTC TGCTTCTGTG
    GAATCCATAC CAGAAGTATT AGATGATAAT GTGTCATTGG CTGATCAATC AGACTCCGCT
    TCAGTCCATG ATATGGATTA TGTAAATCCT AGAGGTGTGA GATTTACCCA ATCTTCACAG
    AAAGAAGGAA CAGTCTTAGT ACCATATGGA CTGCCATGCA TTCGGGAATT ATTTCGTTTT
    CTTATTTCTT TAACCAACCC CCATGATCGA CATAACTCCG AAGTGATGAT ACACATGGGT
    TTACAGTTGA TCACAGTTGC TCTTGAGTCT GCACCTATTG AAAATTATGT TTCGCTTTTA
    GGCCTTGTAA AGGATGAACT TTGTCGACAC TTATTTCAGC TACTGAGCAT TGAGAGACTG
    AACCTATATG CAGCTTCCTT GCGAGCATGT TTTCTTTTGT TTGAGGGTAT GAGGGAGCAT
    TTGAAGTTCC AGCTAGAGAT GTACATAAAG AAGCTCATGG ATATTATTAC TGTGGAGAAC
    CCTAAGATGC CTTATGAGAT GAAAGAAATG GCTCTTGAAG CTATTGTGCA ATTGTGGAGA
    ATCCCGAGCT TTGTTACAGA ATTATACATT AATTACGACT GTGATTATTA TTGCTCCAAC
    CTTTTTGAAG ATCTCACTAA ACTGCTTTCC AAGAATGCCT TTCCAGTTTC TGGACAGCTG
    TACACCACTC ATCTCCTTTC CCTGGAAGCT CTGCTAACTG TCATTGATAG CACAGAATCA
    CACTGCCAGG CTAAAAATCT AAGCAATGTG TCACAGGATA AGAAAGAAAC TGGAAAACCA
    AGTGGAGATC TGTCAGAAGG GACCAAAGAT TCTGGAAATC TCAATGAACA CTCCATTGAA
    GTTAAGCCAT TCACTGTTAG TGCAGAAAAC AATGGATCGA AGCCCCCCAC AAGTGGCCAC
    TTAATGGCAG ATAAAATGAC TCTTGGGGTC CAAGAAACAG AGTACAGCAC TGATGGAGGT
    GAGAAGAAGA CTTTAAAGAA GCCCCAACGC TTTTCCTGCT TACTTCCGAA TGTTCAAGAG
    CTGAATGACA TTAAGACCAA AAAGAAGCTT CTTATAACTG GAACTGAGCA GTTCAACCAG
    AAACCAAAGA AAGGAGTGCA GTTTCTTCAG GAAAAAGACC TTTTGGCAAC CCCCATGGAT
    AATGCTGAGG TAGCACAGTG GCTCAGAGAA AATCCAAGGC TGGACAAGAA AATGATTGGG
    GAGTTTGTGA GTGATCGCAA AAATCTGGAC TTACTTGAAA GTTTTGTAGG AACCTTCCAT
    TTCCAAGGCC TGAGAGTTGA TGAAGCTTTA AGGCTTTACC TTGAAGCTTT TCGTTTGCCT
    GGCGAGGCAC CTGTGATTCA GAGGTTACTG GAGGCTTTTA CAGAGCACTG GCGAAAATCC
    AATGGAACTC CTTTTGCACA CAGTGATGCC TGCTTTGCTT TGGCTTATGC AGTTATCATG
    CTGAACACAG ACCAACACAA TCACAATGTC CGCAAGCAAA ATGTGCCAAT GACTTTAGAG
    GAATTCAGGA AAAATTTAAA GGGTGTTAAT GGTGGAAAAG ATTTTGACCA AGATATGCTA
    GAGGATATTT ATCACGCAAT CAAAAATGAA GAAATTGTAA TGCCTGAAGA GCAGACTGGC
    TTAGTAAAAG AGAATTATTT TTGGAATGTT TTGCTGCATC GGGGAGCCAC TCCAGAAGGA
    ATGTTCTTGC ATGTGGATCC TGGAAGTTAT GACCATGACC TCTTTACTAT GACTTGGGGG
    CCAACAATAG CTGCCTTGTC CTATGTGTTT GATAAGAGCA TGGATGAAAC GATTATTCAG
    AAAGCAATAT CTGGTTTTAG GAAATGTGCA ATGATATCTG CTCACTATGG TCTCAGTGAT
    GTTTTTGACA ATCTCATAAT TTCCTTGTGC AAGTTTACAA CACTTAGCAG TGAAGCCGTT
    GAAAATCTTC CCACTGTTTT TGGAAGCAAC CTAAAAGCTC AGATCGCTGC AAAGACTGTG
    TTCCACTTAT CCCACAGACA TGGTGACATC CTTCGAGAGG GATGGAAAAA TATTATGGAC
    TCCATGCTGC AGCTTTTTAG AGCTGAGCTG CTTCCCAAGG CTATGGTTGA GGTTGAAGAT
    TTTGTAGACC CCAATGGAAA AATATCTCTT CAACGTGAGG AAATCCCTGC CAACCGTGGG
    GAATCCACTG TACTAAGTTT TGTCAGCTGG CTGACTCTGA GTGGGACAGA ACAGTCAAGT
    CTAAGAGGAC CTTCTACTGA AAATCAAGAG GCCAAGAAAC TAGCGCTGGA ATGTATCAAG
    TTATGTGATC CAGAGAAACT GATCACAGAG AGCAAGTTTC TTCAACTGGA GTCTTTGCAA
    GAACTAATGA AGGCTCTGAT ATCTGTGACA CCAGATGAAG AAACGTATGA TGAAGAAGAT
    GCTGCATTCT GCCTAGAAAT GTTAATGAGA ATTGTGCTAG AAAACAGAGA TCGTGTTGGC
    TGTGTTTGGC AGGCTGTACG AGATCATCTC TATCGTCTAT GTGTCCATGC TGTAGAGTTC
    TGCTTCCTGG TTGAAAGAGC AGTTGTAGGG CTGCTGCGCT TGGCAATACG CCTTTTACGC
    AGAGAGGAAA TTAGTGGTCA GGTTATCCTC TCCTTGAGAA TTCTTTTAAT GATGAAACCA
    ACTGTGTTGT CTAAAGTGAG TCGCCAAGTT GCTTTTGGTG TGCATGAGCT CTTGAAAACC
    AATGCTGCTA ACATCCATTC CAGCAATGAC TGGTACACAC TATTCTGCCT TTTGGAGTGC
    ATTGGGGCAG GAGTGAAGCC GCCCCCTGCC TTACAGGTGA CGACGAGAGC TGACAATGAC
    ACAGGTGCGC AGTCTGACAG TGAAGTTTCC TCATACCATC CAAGTGAAGT GAATCTTGAT
    CGTGGATACA CCTCAGACTC TGAGGTATAT GCAGAACACA GCAAGCAGTC AAAAATGCAC
    CGCTCTGTGA CTGATGTGGA TATGATGAAC AGTGGCTGGC TGATGGTTGG AAAGGAAGAT
    GTTGACTCTT CTAAAGTTGC TACAGTTATT AACAAACCTG TTGCTAATGC CCCAGTGAAT
    CAATACAGCC TAACCATTGG TTTGGATTTT GGCCCTCACG ATACCAAATC TCTCATGAAG
    AGCGTGGAGT CCCTTTCTTT TATTGTTCGA GATGCTGCAC ATGTCACACC AGAAAACTTT
    GAACTCTGTG TTAAAACAAT CCGTGTCTTT GTAGAGGCAA GTCTAAATGG AGGTGTGTGG
    TACAAATCTC AGGAGAAAAG GGTAAAGAAC CACAAATATG ACAGTAAATC CAACCGCTTC
    AAAAAGAAAA CCAAGGAAAA AGAAAATTCT GTCCGCCGTA CACGGGCTTC AAATCAGCGA
    CAATACCGAT CTCACAGTGA TGATGAGGAA GATGAAAGTG TACCAGCCAG CTATCACACT
    GTGTCATTAC AGGTTAGTCA TGATCTTTTG GACCTAATGC ACACACTTCA CACAAGAGCT
    GCCACCATAT ACAGTTCTTG GGCAGAGGAA CAGCGCAACC TAGAGACAGC TGCTGAGAAA
    ATTACAGCCG ATTCTAAGAC ACTGTGGTCA AACTGTTGGT GTCCTTTACT GCAAGGTATT
    GCTTGGCTCT GCTGTGATGC TCGGCGCCAG GTCAGAATGC AGGCTCTCAC CTACCTGCAA
    AGGGCACTTT TAGTCCATGA CTTACAGGCT CTTGATGCAC TTGAATGGGA ATCATGCTTT
    AATAAGGTGC TATTTCCATT GCTGACCAAA CTATTGGAAA ATATTAGTCC AGCTGATGTT
    GGCGGGATGG AGGAGACCAG AATGAGAGGC TCCACTCTGC TCTCAAAGGT ATTTCTACAG
    CATCTGTCTC CTCTTCTTTC TCTGTCGACA TTTGCTGCCT TGTGGCTTAC TATTCTTGAT
    TTCATGGACA AGTACATGCA TGCTGGCTCC AGTGACTTAC TGCCTGAGGC AATTCCAGAG
    TCACTGAAAA ATATGCTTCT CGTAATGGAC ACTGCCGGAA TATTCCACAG TGCAGACTCG
    CGGACGGGCT ACTCTGATTT ATGGGAGATC ACGTGGGAAA GGATAGATTG CTTTTTACCT
    CGTCTAAGGG AAGAACTGTT TAAACAAACT GTGATACAAG ATCCACCCAA TATAGTTACA
    ACAGAAACTC CTGTTGAGCC AAGACCTCCC CCTCAATCAA CTCAGTCAGA GAGACCAGCG
    GAGCAGGGAC CTTGTGACTC AGAGACTCAA AGTTTAGCAA TCTCATCTGC TCCAGTAGTT
    GGATCAAGTT CTTCCTGCAC CCCAGTTAGA GTCAGCCCTG TAACAGATAT ACCTCCACCT
    ATAACTCAGT CTCCAGTCAT CCTGCATCCT CTGACCTCCC CGCTGCAGGT GGGAGTGCCA
    CCCATGTCGC TGCCAATCAT CCTCAACCCT GCATTAATTG AAGCTACCTC TCCAGTTCCG
    CTCTTGTCCA CTCCCCGACC TGCTGATGTA TCATCTGTCT CAGAGGTCAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012822306.1
    CDS100..5673
    Translation

    Target ORF information:

    RefSeq Version XM_012966852.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966852.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGT TGAAAATGAG AGCTGGAGGA
    ATGAGTGACT CCTCAAAATG GAAAAAGCAG AAGAAATCTC CTAGGCAGCC ACGTCATGTT
    ACCAAAGCTA TGGCGGCAGC AGAACCCTCC TCTCAGCAAC CAGTAGTTAA TGGCACCACA
    ATTACTACTA ACCTCACTGG TGAAGTTCCT TTTATTGATG CTTCCTCTCC AGATGCTAAT
    TTTCCCATTT CAGGGGTGAG TTCAGAGGCC ACGTCTGCTG TAGTCAGTCC TACCACAGAT
    AGTGGTGTAG ATATGTCCTC TCAGGCAACA TCTAAAGAAG ACCTTTCTGA ACTGGATCCT
    GCAAGTCCAT CTCTTTCTGC CATTCAGCAA TCAGGAGACA TTTGTCTTTC TGAACAGCAA
    AACCAGGATG ATGATAAGAT TGAAGACCTT ATGGAAAGGC ATACATATTC TGCTTCTGTG
    GAATCCATAC CAGAAGTATT AGATGATAAT GTGTCATTGG CTGATCAATC AGACTCCGCT
    TCAGTCCATG ATATGGATTA TGTAAATCCT AGAGGTGTGA GATTTACCCA ATCTTCACAG
    AAAGAAGGAA CAGTCTTAGT ACCATATGGA CTGCCATGCA TTCGGGAATT ATTTCGTTTT
    CTTATTTCTT TAACCAACCC CCATGATCGA CATAACTCCG AAGTGATGAT ACACATGGGT
    TTACAGTTGA TCACAGTTGC TCTTGAGTCT GCACCTATTG AAAATTATGT TTCGCTTTTA
    GGCCTTGTAA AGGATGAACT TTGTCGACAC TTATTTCAGC TACTGAGCAT TGAGAGACTG
    AACCTATATG CAGCTTCCTT GCGAGCATGT TTTCTTTTGT TTGAGGGTAT GAGGGAGCAT
    TTGAAGTTCC AGCTAGAGAT GTACATAAAG AAGCTCATGG ATATTATTAC TGTGGAGAAC
    CCTAAGATGC CTTATGAGAT GAAAGAAATG GCTCTTGAAG CTATTGTGCA ATTGTGGAGA
    ATCCCGAGCT TTGTTACAGA ATTATACATT AATTACGACT GTGATTATTA TTGCTCCAAC
    CTTTTTGAAG ATCTCACTAA ACTGCTTTCC AAGAATGCCT TTCCAGTTTC TGGACAGCTG
    TACACCACTC ATCTCCTTTC CCTGGAAGCT CTGCTAACTG TCATTGATAG CACAGAATCA
    CACTGCCAGG CTAAAAATCT AAGCAATGTG TCACAGGATA AGAAAGAAAC TGGAAAACCA
    AGTGGAGATC TGTCAGAAGG GACCAAAGAT TCTGGAAATC TCAATGAACA CTCCATTGAA
    GTTAAGCCAT TCACTGTTAG TGCAGAAAAC AATGGATCGA AGCCCCCCAC AAGTGGCCAC
    TTAATGGCAG ATAAAATGAC TCTTGGGGTC CAAGAAACAG AGTACAGCAC TGATGGAGGT
    GAGAAGAAGA CTTTAAAGAA GCCCCAACGC TTTTCCTGCT TACTTCCGAA TGTTCAAGAG
    CTGAATGACA TTAAGACCAA AAAGAAGCTT CTTATAACTG GAACTGAGCA GTTCAACCAG
    AAACCAAAGA AAGGAGTGCA GTTTCTTCAG GAAAAAGACC TTTTGGCAAC CCCCATGGAT
    AATGCTGAGG TAGCACAGTG GCTCAGAGAA AATCCAAGGC TGGACAAGAA AATGATTGGG
    GAGTTTGTGA GTGATCGCAA AAATCTGGAC TTACTTGAAA GTTTTGTAGG AACCTTCCAT
    TTCCAAGGCC TGAGAGTTGA TGAAGCTTTA AGGCTTTACC TTGAAGCTTT TCGTTTGCCT
    GGCGAGGCAC CTGTGATTCA GAGGTTACTG GAGGCTTTTA CAGAGCACTG GCGAAAATCC
    AATGGAACTC CTTTTGCACA CAGTGATGCC TGCTTTGCTT TGGCTTATGC AGTTATCATG
    CTGAACACAG ACCAACACAA TCACAATGTC CGCAAGCAAA ATGTGCCAAT GACTTTAGAG
    GAATTCAGGA AAAATTTAAA GGGTGTTAAT GGTGGAAAAG ATTTTGACCA AGATATGCTA
    GAGGATATTT ATCACGCAAT CAAAAATGAA GAAATTGTAA TGCCTGAAGA GCAGACTGGC
    TTAGTAAAAG AGAATTATTT TTGGAATGTT TTGCTGCATC GGGGAGCCAC TCCAGAAGGA
    ATGTTCTTGC ATGTGGATCC TGGAAGTTAT GACCATGACC TCTTTACTAT GACTTGGGGG
    CCAACAATAG CTGCCTTGTC CTATGTGTTT GATAAGAGCA TGGATGAAAC GATTATTCAG
    AAAGCAATAT CTGGTTTTAG GAAATGTGCA ATGATATCTG CTCACTATGG TCTCAGTGAT
    GTTTTTGACA ATCTCATAAT TTCCTTGTGC AAGTTTACAA CACTTAGCAG TGAAGCCGTT
    GAAAATCTTC CCACTGTTTT TGGAAGCAAC CTAAAAGCTC AGATCGCTGC AAAGACTGTG
    TTCCACTTAT CCCACAGACA TGGTGACATC CTTCGAGAGG GATGGAAAAA TATTATGGAC
    TCCATGCTGC AGCTTTTTAG AGCTGAGCTG CTTCCCAAGG CTATGGTTGA GGTTGAAGAT
    TTTGTAGACC CCAATGGAAA AATATCTCTT CAACGTGAGG AAATCCCTGC CAACCGTGGG
    GAATCCACTG TACTAAGTTT TGTCAGCTGG CTGACTCTGA GTGGGACAGA ACAGTCAAGT
    CTAAGAGGAC CTTCTACTGA AAATCAAGAG GCCAAGAAAC TAGCGCTGGA ATGTATCAAG
    TTATGTGATC CAGAGAAACT GATCACAGAG AGCAAGTTTC TTCAACTGGA GTCTTTGCAA
    GAACTAATGA AGGCTCTGAT ATCTGTGACA CCAGATGAAG AAACGTATGA TGAAGAAGAT
    GCTGCATTCT GCCTAGAAAT GTTAATGAGA ATTGTGCTAG AAAACAGAGA TCGTGTTGGC
    TGTGTTTGGC AGGCTGTACG AGATCATCTC TATCGTCTAT GTGTCCATGC TGTAGAGTTC
    TGCTTCCTGG TTGAAAGAGC AGTTGTAGGG CTGCTGCGCT TGGCAATACG CCTTTTACGC
    AGAGAGGAAA TTAGTGGTCA GGTTATCCTC TCCTTGAGAA TTCTTTTAAT GATGAAACCA
    ACTGTGTTGT CTAAAGTGAG TCGCCAAGTT GCTTTTGGTG TGCATGAGCT CTTGAAAACC
    AATGCTGCTA ACATCCATTC CAGCAATGAC TGGTACACAC TATTCTGCCT TTTGGAGTGC
    ATTGGGGCAG GAGTGAAGCC GCCCCCTGCC TTACAGGTGA CGACGAGAGC TGACAATGAC
    ACAGGTGCGC AGTCTGACAG TGAAGTTTCC TCATACCATC CAAGTGAAGT GAATCTTGAT
    CGTGGATACA CCTCAGACTC TGAGGTATAT GCAGAACACA GCAAGCAGTC AAAAATGCAC
    CGCTCTGTGA CTGATGTGGA TATGATGAAC AGTGGCTGGC TGATGGTTGG AAAGGAAGAT
    GTTGACTCTT CTAAAGTTGC TACAGTTATT AACAAACCTG TTGCTAATGC CCCAGTGAAT
    CAATACAGCC TAACCATTGG TTTGGATTTT GGCCCTCACG ATACCAAATC TCTCATGAAG
    AGCGTGGAGT CCCTTTCTTT TATTGTTCGA GATGCTGCAC ATGTCACACC AGAAAACTTT
    GAACTCTGTG TTAAAACAAT CCGTGTCTTT GTAGAGGCAA GTCTAAATGG AGGTGTGTGG
    TACAAATCTC AGGAGAAAAG GGTAAAGAAC CACAAATATG ACAGTAAATC CAACCGCTTC
    AAAAAGAAAA CCAAGGAAAA AGAAAATTCT GTCCGCCGTA CACGGGCTTC AAATCAGCGA
    CAATACCGAT CTCACAGTGA TGATGAGGAA GATGAAAGTG TACCAGCCAG CTATCACACT
    GTGTCATTAC AGGTTAGTCA TGATCTTTTG GACCTAATGC ACACACTTCA CACAAGAGCT
    GCCACCATAT ACAGTTCTTG GGCAGAGGAA CAGCGCAACC TAGAGACAGC TGCTGAGAAA
    ATTACAGCCG ATTCTAAGAC ACTGTGGTCA AACTGTTGGT GTCCTTTACT GCAAGGTATT
    GCTTGGCTCT GCTGTGATGC TCGGCGCCAG GTCAGAATGC AGGCTCTCAC CTACCTGCAA
    AGGGCACTTT TAGTCCATGA CTTACAGGCT CTTGATGCAC TTGAATGGGA ATCATGCTTT
    AATAAGGTGC TATTTCCATT GCTGACCAAA CTATTGGAAA ATATTAGTCC AGCTGATGTT
    GGCGGGATGG AGGAGACCAG AATGAGAGGC TCCACTCTGC TCTCAAAGGT ATTTCTACAG
    CATCTGTCTC CTCTTCTTTC TCTGTCGACA TTTGCTGCCT TGTGGCTTAC TATTCTTGAT
    TTCATGGACA AGTACATGCA TGCTGGCTCC AGTGACTTAC TGCCTGAGGC AATTCCAGAG
    TCACTGAAAA ATATGCTTCT CGTAATGGAC ACTGCCGGAA TATTCCACAG TGCAGACTCG
    CGGACGGGCT ACTCTGATTT ATGGGAGATC ACGTGGGAAA GGATAGATTG CTTTTTACCT
    CGTCTAAGGG AAGAACTGTT TAAACAAACT GTGATACAAG ATCCACCCAA TATAGTTACA
    ACAGAAACTC CTGTTGAGCC AAGACCTCCC CCTCAATCAA CTCAGTCAGA GAGACCAGCG
    GAGCAGGGAC CTTGTGACTC AGAGACTCAA AGTTTAGCAA TCTCATCTGC TCCAGTAGTT
    GGATCAAGTT CTTCCTGCAC CCCAGTTAGA GTCAGCCCTG TAACAGATAT ACCTCCACCT
    ATAACTCAGT CTCCAGTCAT CCTGCATCCT CTGACCTCCC CGCTGCAGGT GGGAGTGCCA
    CCCATGTCGC TGCCAATCAT CCTCAACCCT GCATTAATTG AAGCTACCTC TCCAGTTCCG
    CTCTTGTCCA CTCCCCGACC TGCTGATGTA TCATCTGTCT CAGAGGTCAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa62539
    Clone ID Related Accession (Same CDS sequence) XM_031906086.1
    Accession Version XM_031906086.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4989bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCCATTTT TCCTTGAGGC TTACAATATC TTTTGGAAAA ACAAACGTGT TCAGTTGAAA 
    ATGAGAGCTG GAGGAATGAG TGACTCCTCA AAATGGAAAA AGCAGAAGAA ATCTCCTAGG
    CAGCCACGTC ATGTTACCAA AGCTATGGCG GCAGCAGAAC CCTCCTCTCA GCAACCAGTA
    GTTAATGGCA CCACAATTAC TACTAACCTC ACTGGTGAAG TTCCTTTTAT TGATGCTTCC
    TCTCCAGATG CTAATTTTCC CATTTCAGGG GTGAGTTCAG AGGCCACGTC TGCTGTAGTC
    AGTCCTACCA CAGATAGTGG TGTAGATATG TCCTCTCAGG CAACATCTAA AGAAGACCTT
    TCTGAACTGG ATCCTGCAAG TCCATCTCTT TCTGCCATTC AGCAATCAGG AGACATTTGT
    CTTTCTGAAC AGCAAAACCA GGATGATGAT AAGATTGAAG ACCTTATGGA AAGGCATACA
    TATTCTGCTT CTGTGGAATC CATACCAGAA GTATTAGATG ATAATGTGTC ATTGGCTGAT
    CAATCAGACT CCGCTTCAGT CCATGATATG GATTATGTAA ATCCTAGAGG TGTGAGATTT
    ACCCAATCTT CACAGAAAGA AGGAACAGTC TTAGTACCAT ATGGACTGCC ATGCATTCGG
    GAATTATTTC GTTTTCTTAT TTCTTTAACC AACCCCCATG ATCGACATAA CTCCGAAGTG
    ATGATACACA TGGGTTTACA GTTGATCACA GTTGCTCTTG AGTCTGCACC TATTGAAAAT
    TATGTTTCGC TTTTAGGCCT TGTAAAGGAT GAACTTTGTC GACACTTATT TCAGCTACTG
    AGCATTGAGA GACTGAACCT ATATGCAGCT TCCTTGCGAG CATGTTTTCT TTTGTTTGAG
    GGTATGAGGG AGCATTTGAA GTTCCAGCTA GAGATGTACA TAAAGAAGCT CATGGATATT
    ATTACTGTGG AGAACCCTAA GATGCCTTAT GAGATGAAAG AAATGGCTCT TGAAGCTATT
    GTGCAATTGT GGAGAATCCC GAGCTTTGTT ACAGAATTAT ACATTAATTA CGACTGTGAT
    TATTATTGCT CCAACCTTTT TGAAGATCTC ACTAAACTGC TTTCCAAGAA TGCCTTTCCA
    GTTTCTGGAC AGCTGTACAC CACTCATCTC CTTTCCCTGG AAGCTCTGCT AACTGTCATT
    GATAGCACAG AATCACACTG CCAGGCTAAA AATCTAAGCA ATGTGTCACA GGATAAGAAA
    GAAACTGGAA AACCAAGTGG AGATCTGTCA GAAGGGACCA AAGATTCTGG AAATCTCAAT
    GAACACTCCA TTGAAGTTAA GCCATTCACT GTTAGTGCAG AAAACAATGG ATCGAAGCCC
    CCCACAAGTG GCCACTTAAT GGCAGATAAA ATGACTCTTG GGGTCCAAGA AACAGAGTAC
    AGCACTGATG GAGGTGAGAA GAAGACTTTA AAGAAGCCCC AACGCTTTTC CTGCTTACTT
    CCGAATGTTC AAGAGCTGAA TGACATTAAG ACCAAAAAGA AGCTTCTTAT AACTGGAACT
    GAGCAGTTCA ACCAGAAACC AAAGAAAGGA GTGCAGTTTC TTCAGGAAAA AGACCTTTTG
    GCAACCCCCA TGGATAATGC TGAGGTAGCA CAGTGGCTCA GAGAAAATCC AAGGCTGGAC
    AAGAAAATGA TTGGGGAGTT TGTGAGTGAT CGCAAAAATC TGGACTTACT TGAAAGTTTT
    GTAGGAACCT TCCATTTCCA AGGCCTGAGA GTTGATGAAG CTTTAAGGCT TTACCTTGAA
    GCTTTTCGTT TGCCTGGCGA GGCACCTGTG ATTCAGAGGT TACTGGAGGC TTTTACAGAG
    CACTGGCGAA AATCCAATGG AACTCCTTTT GCACACAGTG ATGCCTGCTT TGCTTTGGCT
    TATGCAGTTA TCATGCTGAA CACAGACCAA CACAATCACA ATGTCCGCAA GCAAAATGTG
    CCAATGACTT TAGAGGAATT CAGGAAAAAT TTAAAGGGTG TTAATGGTGG AAAAGATTTT
    GACCAAGATA TGCTAGAGGA TATTTATCAC GCAATCAAAA ATGAAGAAAT TGTAATGCCT
    GAAGAGCAGA CTGGCTTAGT AAAAGAGAAT TATTTTTGGA ATGTTTTGCT GCATCGGGGA
    GCCACTCCAG AAGGAATGTT CTTGCATGTG GATCCTGGAA GTTATGACCA TGACCTCTTT
    ACTATGACTT GGGGGCCAAC AATAGCTGCC TTGTCCTATG TGTTTGATAA GAGCATGGAT
    GAAACGATTA TTCAGAAAGC AATATCTGGT TTTAGGAAAT GTGCAATGAT ATCTGCTCAC
    TATGGTCTCA GTGATGTTTT TGACAATCTC ATAATTTCCT TGTGCAAGTT TACAACACTT
    AGCAGTGAAG CCGTTGAAAA TCTTCCCACT GTTTTTGGAA GCAACCTAAA AGCTCAGATC
    GCTGCAAAGA CTGTGTTCCA CTTATCCCAC AGACATGGTG ACATCCTTCG AGAGGGATGG
    AAAAATATTA TGGACTCCAT GCTGCAGCTT TTTAGAGCTG AGCTGCTTCC CAAGGCTATG
    GTTGAGGTTG AAGATTTTGT AGACCCCAAT GGAAAAATAT CTCTTCAACG TGAGGAAATC
    CCTGCCAACC GTGGGGAATC CACTGTACTA AGTTTTGTCA GCTGGCTGAC TCTGAGTGGG
    ACAGAACAGT CAAGTCTAAG AGGACCTTCT ACTGAAAATC AAGAGGCCAA GAAACTAGCG
    CTGGAATGTA TCAAGTTATG TGATCCAGAG AAACTGATCA CAGAGAGCAA GTTTCTTCAA
    CTGGAGTCTT TGCAAGAACT AATGAAGGCT CTGATATCTG TGACACCAGA TGAAGAAACG
    TATGATGAAG AAGATGCTGC ATTCTGCCTA GAAATGTTAA TGAGAATTGT GCTAGAAAAC
    AGAGATCGTG TTGGCTGTGT TTGGCAGGCT GTACGAGATC ATCTCTATCG TCTATGTGTC
    CATGCTGTAG AGTTCTGCTT CCTGGTTGAA AGAGCAGTTG TAGGGCTGCT GCGCTTGGCA
    ATACGCCTTT TACGCAGAGA GGAAATTAGT GGTCAGGTTA TCCTCTCCTT GAGAATTCTT
    TTAATGATGA AACCAACTGT GTTGTCTAAA GTGAGTCGCC AAGTTGCTTT TGGTGTGCAT
    GAGCTCTTGA AAACCAATGC TGCTAACATC CATTCCAGCA ATGACTGGTA CACACTATTC
    TGCCTTTTGG AGTGCATTGG GGCAGGAGTG AAGCCGCCCC CTGCCTTACA GGTGACGACG
    AGAGCTGACA ATGACACAGG TGCGCAGTCT GACAGTGAAG TTTCCTCATA CCATCCAAGT
    GAAGTGAATC TTGATCGTGG ATACACCTCA GACTCTGAGG TATATGCAGA ACACAGCAAG
    CAGTCAAAAA TGCACCGCTC TGTGACTGAT GTGGATATGA TGAACAGTGG CTGGCTGATG
    GTTGGAAAGG AAGATGTTGA CTCTTCTAAA GTTGCTACAG TTATTAACAA ACCTGTTGCT
    AATGCCCCAG TGAATCAATA CAGCCTAACC ATTGGTTTGG ATTTTGGCCC TCACGATACC
    AAATCTCTCA TGAAGAGCGT GGAGTCCCTT TCTTTTATTG TTCGAGATGC TGCACATGTC
    ACACCAGAAA ACTTTGAACT CTGTGTTAAA ACAATCCGTG TCTTTGTAGA GGCAAGTCTA
    AATGGAGGTG TGTGGTACAA ATCTCAGGAG AAAAGGGTAA AGAACCACAA ATATGACAGT
    AAATCCAACC GCTTCAAAAA GAAAACCAAG GAAAAAGAAA ATTCTGTCCG CCGTACACGG
    GCTTCAAATC AGCGACAATA CCGATCTCAC AGTGATGATG AGGAAGATGA AAGTGTACCA
    GCCAGCTATC ACACTGTGTC ATTACAGGTT AGTCATGATC TTTTGGACCT AATGCACACA
    CTTCACACAA GAGCTGCCAC CATATACAGT TCTTGGGCAG AGGAACAGCG CAACCTAGAG
    ACAGCTGCTG AGAAAATTAC AGCCGATTCT AAGACACTGT GGTCAAACTG TTGGTGTCCT
    TTACTGCAAG GTATTGCTTG GCTCTGCTGT GATGCTCGGC GCCAGGTCAG AATGCAGGCT
    CTCACCTACC TGCAAAGGGC ACTTTTAGTC CATGACTTAC AGGCTCTTGA TGCACTTGAA
    TGGGAATCAT GCTTTAATAA GGTGCTATTT CCATTGCTGA CCAAACTATT GGAAAATATT
    AGTCCAGCTG ATGTTGGCGG GATGGAGGAG ACCAGAATGA GAGGCTCCAC TCTGCTCTCA
    AAGGTATTTC TACAGCATCT GTCTCCTCTT CTTTCTCTGT CGACATTTGC TGCCTTGTGG
    CTTACTATTC TTGATTTCAT GGACAAGTAC ATGCATGCTG GCTCCAGTGA CTTACTGCCT
    GAGGCAATTC CAGAGTCACT GAAAAATATG CTTCTCGTAA TGGACACTGC CGGAATATTC
    CACAGTGCAG ACTCGCGGAC GGGCTACTCT GATTTATGGG AGATCACGTG GGAAAGGATA
    GATTGCTTTT TACCTCGTCT AAGGGAAGAA CTGTTTAAAC AAACTGTGAT ACAAGATCCA
    CCCAATATAG TTACAACAGA AACTCCTGTT GAGCCAAGAC CTCCCCCTCA ATCAACTCAG
    TCAGAGAGAC CAGCGGAGCA GGGACCTTGT GACTCAGAGA CTCAAAGTTT AGCAATCTCA
    TCTGCTCCAG TAGTTGGATC AAGTTCTTCC TGCACCCCAG TTAGAGTCAG CCCTGTAACA
    GATATACCTC CACCTATAAC TCAGTCTCCA GTCATCCTGC ATCCTCTGAC CTCCCCGCTG
    CAGGTGGGAG TGCCACCCAT GTCGCTGCCA ATCATCCTCA ACCCTGCATT AATTGAAGCT
    ACCTCTCCAG TTCCGCTCTT GTCCACTCCC CGACCTGCTG ATGTATCATC TGTCTCAGAG
    GTCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031761946.1
    CDS16..5004
    Translation

    Target ORF information:

    RefSeq Version XM_031906086.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031906086.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCCATTTT TCCTTGAGGC TTACAATATC TTTTGGAAAA ACAAACGTGT TCAGTTGAAA 
    ATGAGAGCTG GAGGAATGAG TGACTCCTCA AAATGGAAAA AGCAGAAGAA ATCTCCTAGG
    CAGCCACGTC ATGTTACCAA AGCTATGGCG GCAGCAGAAC CCTCCTCTCA GCAACCAGTA
    GTTAATGGCA CCACAATTAC TACTAACCTC ACTGGTGAAG TTCCTTTTAT TGATGCTTCC
    TCTCCAGATG CTAATTTTCC CATTTCAGGG GTGAGTTCAG AGGCCACGTC TGCTGTAGTC
    AGTCCTACCA CAGATAGTGG TGTAGATATG TCCTCTCAGG CAACATCTAA AGAAGACCTT
    TCTGAACTGG ATCCTGCAAG TCCATCTCTT TCTGCCATTC AGCAATCAGG AGACATTTGT
    CTTTCTGAAC AGCAAAACCA GGATGATGAT AAGATTGAAG ACCTTATGGA AAGGCATACA
    TATTCTGCTT CTGTGGAATC CATACCAGAA GTATTAGATG ATAATGTGTC ATTGGCTGAT
    CAATCAGACT CCGCTTCAGT CCATGATATG GATTATGTAA ATCCTAGAGG TGTGAGATTT
    ACCCAATCTT CACAGAAAGA AGGAACAGTC TTAGTACCAT ATGGACTGCC ATGCATTCGG
    GAATTATTTC GTTTTCTTAT TTCTTTAACC AACCCCCATG ATCGACATAA CTCCGAAGTG
    ATGATACACA TGGGTTTACA GTTGATCACA GTTGCTCTTG AGTCTGCACC TATTGAAAAT
    TATGTTTCGC TTTTAGGCCT TGTAAAGGAT GAACTTTGTC GACACTTATT TCAGCTACTG
    AGCATTGAGA GACTGAACCT ATATGCAGCT TCCTTGCGAG CATGTTTTCT TTTGTTTGAG
    GGTATGAGGG AGCATTTGAA GTTCCAGCTA GAGATGTACA TAAAGAAGCT CATGGATATT
    ATTACTGTGG AGAACCCTAA GATGCCTTAT GAGATGAAAG AAATGGCTCT TGAAGCTATT
    GTGCAATTGT GGAGAATCCC GAGCTTTGTT ACAGAATTAT ACATTAATTA CGACTGTGAT
    TATTATTGCT CCAACCTTTT TGAAGATCTC ACTAAACTGC TTTCCAAGAA TGCCTTTCCA
    GTTTCTGGAC AGCTGTACAC CACTCATCTC CTTTCCCTGG AAGCTCTGCT AACTGTCATT
    GATAGCACAG AATCACACTG CCAGGCTAAA AATCTAAGCA ATGTGTCACA GGATAAGAAA
    GAAACTGGAA AACCAAGTGG AGATCTGTCA GAAGGGACCA AAGATTCTGG AAATCTCAAT
    GAACACTCCA TTGAAGTTAA GCCATTCACT GTTAGTGCAG AAAACAATGG ATCGAAGCCC
    CCCACAAGTG GCCACTTAAT GGCAGATAAA ATGACTCTTG GGGTCCAAGA AACAGAGTAC
    AGCACTGATG GAGGTGAGAA GAAGACTTTA AAGAAGCCCC AACGCTTTTC CTGCTTACTT
    CCGAATGTTC AAGAGCTGAA TGACATTAAG ACCAAAAAGA AGCTTCTTAT AACTGGAACT
    GAGCAGTTCA ACCAGAAACC AAAGAAAGGA GTGCAGTTTC TTCAGGAAAA AGACCTTTTG
    GCAACCCCCA TGGATAATGC TGAGGTAGCA CAGTGGCTCA GAGAAAATCC AAGGCTGGAC
    AAGAAAATGA TTGGGGAGTT TGTGAGTGAT CGCAAAAATC TGGACTTACT TGAAAGTTTT
    GTAGGAACCT TCCATTTCCA AGGCCTGAGA GTTGATGAAG CTTTAAGGCT TTACCTTGAA
    GCTTTTCGTT TGCCTGGCGA GGCACCTGTG ATTCAGAGGT TACTGGAGGC TTTTACAGAG
    CACTGGCGAA AATCCAATGG AACTCCTTTT GCACACAGTG ATGCCTGCTT TGCTTTGGCT
    TATGCAGTTA TCATGCTGAA CACAGACCAA CACAATCACA ATGTCCGCAA GCAAAATGTG
    CCAATGACTT TAGAGGAATT CAGGAAAAAT TTAAAGGGTG TTAATGGTGG AAAAGATTTT
    GACCAAGATA TGCTAGAGGA TATTTATCAC GCAATCAAAA ATGAAGAAAT TGTAATGCCT
    GAAGAGCAGA CTGGCTTAGT AAAAGAGAAT TATTTTTGGA ATGTTTTGCT GCATCGGGGA
    GCCACTCCAG AAGGAATGTT CTTGCATGTG GATCCTGGAA GTTATGACCA TGACCTCTTT
    ACTATGACTT GGGGGCCAAC AATAGCTGCC TTGTCCTATG TGTTTGATAA GAGCATGGAT
    GAAACGATTA TTCAGAAAGC AATATCTGGT TTTAGGAAAT GTGCAATGAT ATCTGCTCAC
    TATGGTCTCA GTGATGTTTT TGACAATCTC ATAATTTCCT TGTGCAAGTT TACAACACTT
    AGCAGTGAAG CCGTTGAAAA TCTTCCCACT GTTTTTGGAA GCAACCTAAA AGCTCAGATC
    GCTGCAAAGA CTGTGTTCCA CTTATCCCAC AGACATGGTG ACATCCTTCG AGAGGGATGG
    AAAAATATTA TGGACTCCAT GCTGCAGCTT TTTAGAGCTG AGCTGCTTCC CAAGGCTATG
    GTTGAGGTTG AAGATTTTGT AGACCCCAAT GGAAAAATAT CTCTTCAACG TGAGGAAATC
    CCTGCCAACC GTGGGGAATC CACTGTACTA AGTTTTGTCA GCTGGCTGAC TCTGAGTGGG
    ACAGAACAGT CAAGTCTAAG AGGACCTTCT ACTGAAAATC AAGAGGCCAA GAAACTAGCG
    CTGGAATGTA TCAAGTTATG TGATCCAGAG AAACTGATCA CAGAGAGCAA GTTTCTTCAA
    CTGGAGTCTT TGCAAGAACT AATGAAGGCT CTGATATCTG TGACACCAGA TGAAGAAACG
    TATGATGAAG AAGATGCTGC ATTCTGCCTA GAAATGTTAA TGAGAATTGT GCTAGAAAAC
    AGAGATCGTG TTGGCTGTGT TTGGCAGGCT GTACGAGATC ATCTCTATCG TCTATGTGTC
    CATGCTGTAG AGTTCTGCTT CCTGGTTGAA AGAGCAGTTG TAGGGCTGCT GCGCTTGGCA
    ATACGCCTTT TACGCAGAGA GGAAATTAGT GGTCAGGTTA TCCTCTCCTT GAGAATTCTT
    TTAATGATGA AACCAACTGT GTTGTCTAAA GTGAGTCGCC AAGTTGCTTT TGGTGTGCAT
    GAGCTCTTGA AAACCAATGC TGCTAACATC CATTCCAGCA ATGACTGGTA CACACTATTC
    TGCCTTTTGG AGTGCATTGG GGCAGGAGTG AAGCCGCCCC CTGCCTTACA GGTGACGACG
    AGAGCTGACA ATGACACAGG TGCGCAGTCT GACAGTGAAG TTTCCTCATA CCATCCAAGT
    GAAGTGAATC TTGATCGTGG ATACACCTCA GACTCTGAGG TATATGCAGA ACACAGCAAG
    CAGTCAAAAA TGCACCGCTC TGTGACTGAT GTGGATATGA TGAACAGTGG CTGGCTGATG
    GTTGGAAAGG AAGATGTTGA CTCTTCTAAA GTTGCTACAG TTATTAACAA ACCTGTTGCT
    AATGCCCCAG TGAATCAATA CAGCCTAACC ATTGGTTTGG ATTTTGGCCC TCACGATACC
    AAATCTCTCA TGAAGAGCGT GGAGTCCCTT TCTTTTATTG TTCGAGATGC TGCACATGTC
    ACACCAGAAA ACTTTGAACT CTGTGTTAAA ACAATCCGTG TCTTTGTAGA GGCAAGTCTA
    AATGGAGGTG TGTGGTACAA ATCTCAGGAG AAAAGGGTAA AGAACCACAA ATATGACAGT
    AAATCCAACC GCTTCAAAAA GAAAACCAAG GAAAAAGAAA ATTCTGTCCG CCGTACACGG
    GCTTCAAATC AGCGACAATA CCGATCTCAC AGTGATGATG AGGAAGATGA AAGTGTACCA
    GCCAGCTATC ACACTGTGTC ATTACAGGTT AGTCATGATC TTTTGGACCT AATGCACACA
    CTTCACACAA GAGCTGCCAC CATATACAGT TCTTGGGCAG AGGAACAGCG CAACCTAGAG
    ACAGCTGCTG AGAAAATTAC AGCCGATTCT AAGACACTGT GGTCAAACTG TTGGTGTCCT
    TTACTGCAAG GTATTGCTTG GCTCTGCTGT GATGCTCGGC GCCAGGTCAG AATGCAGGCT
    CTCACCTACC TGCAAAGGGC ACTTTTAGTC CATGACTTAC AGGCTCTTGA TGCACTTGAA
    TGGGAATCAT GCTTTAATAA GGTGCTATTT CCATTGCTGA CCAAACTATT GGAAAATATT
    AGTCCAGCTG ATGTTGGCGG GATGGAGGAG ACCAGAATGA GAGGCTCCAC TCTGCTCTCA
    AAGGTATTTC TACAGCATCT GTCTCCTCTT CTTTCTCTGT CGACATTTGC TGCCTTGTGG
    CTTACTATTC TTGATTTCAT GGACAAGTAC ATGCATGCTG GCTCCAGTGA CTTACTGCCT
    GAGGCAATTC CAGAGTCACT GAAAAATATG CTTCTCGTAA TGGACACTGC CGGAATATTC
    CACAGTGCAG ACTCGCGGAC GGGCTACTCT GATTTATGGG AGATCACGTG GGAAAGGATA
    GATTGCTTTT TACCTCGTCT AAGGGAAGAA CTGTTTAAAC AAACTGTGAT ACAAGATCCA
    CCCAATATAG TTACAACAGA AACTCCTGTT GAGCCAAGAC CTCCCCCTCA ATCAACTCAG
    TCAGAGAGAC CAGCGGAGCA GGGACCTTGT GACTCAGAGA CTCAAAGTTT AGCAATCTCA
    TCTGCTCCAG TAGTTGGATC AAGTTCTTCC TGCACCCCAG TTAGAGTCAG CCCTGTAACA
    GATATACCTC CACCTATAAC TCAGTCTCCA GTCATCCTGC ATCCTCTGAC CTCCCCGCTG
    CAGGTGGGAG TGCCACCCAT GTCGCTGCCA ATCATCCTCA ACCCTGCATT AATTGAAGCT
    ACCTCTCCAG TTCCGCTCTT GTCCACTCCC CGACCTGCTG ATGTATCATC TGTCTCAGAG
    GTCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa62540
    Clone ID Related Accession (Same CDS sequence) XM_018095873.2
    Accession Version XM_018095873.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5556bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018095873.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGT TGAAAATGAG AGCTGGAGGA
    ATGAGTGACT CCTCAAAATG GAAAAAGCAG AAGAAATCTC CTAGGCAGCC ACGTCATGTT
    ACCAAAGCTA TGGCGGCAGC AGAACCCTCC TCTCAGCAAC CAGTAGTTAA TGGCACCACA
    ATTACTACTA ACCTCACTGG TGAAGTTCCT TTTATTGATG CTTCCTCTCC AGATGCTAAT
    TTTCCCATTT CAGGGGTGAG TTCAGAGGCC ACGTCTGCTG TAGTCAGTCC TACCACAGAT
    AGTGGTGTAG ATATGTCCTC TCAGGCAACA TCTAAAGAAG ACCTTTCTGA ACTGGATCCT
    GCAAGTCCAT CTCTTTCTGC CATTCAGCAA TCAGGAGACA TTTGTCTTTC TGAACAGCAA
    AACCAGGATG ATGATAAGAT TGAAGACCTT ATGGAAAGGC ATACATATTC TGCTTCTGTG
    GAATCCATAC CAGAAGTATT AGATGATAAT GTGTCATTGG CTGATCAATC AGACTCCGCT
    TCAGTCCATG ATATGGATTA TGTAAATCCT AGAGGTGTGA GATTTACCCA ATCTTCACAG
    AAAGAAGGAA CAGTCTTAGT ACCATATGGA CTGCCATGCA TTCGGGAATT ATTTCGTTTT
    CTTATTTCTT TAACCAACCC CCATGATCGA CATAACTCCG AAGTGATGAT ACACATGGGT
    TTACAGTTGA TCACAGTTGC TCTTGAGTCT GCACCTATTG AAAATTATGT TTCGCTTTTA
    GGCCTTGTAA AGGATGAACT TTGTCGACAC TTATTTCAGC TACTGAGCAT TGAGAGACTG
    AACCTATATG CAGCTTCCTT GCGAGCATGT TTTCTTTTGT TTGAGGGTAT GAGGGAGCAT
    TTGAAGTTCC AGCTAGAGAT GTACATAAAG AAGCTCATGG ATATTATTAC TGTGGAGAAC
    CCTAAGATGC CTTATGAGAT GAAAGAAATG GCTCTTGAAG CTATTGTGCA ATTGTGGAGA
    ATCCCGAGCT TTGTTACAGA ATTATACATT AATTACGACT GTGATTATTA TTGCTCCAAC
    CTTTTTGAAG ATCTCACTAA ACTGCTTTCC AAGAATGCCT TTCCAGTTTC TGGACAGCTG
    TACACCACTC ATCTCCTTTC CCTGGAAGCT CTGCTAACTG TCATTGATAG CACAGAATCA
    CACTGCCAGG CTAAAAATCT AAGCAATGTG TCACAGGATA AGAAAGAAAC TGGAAAACCA
    AGTGGAGATC TGTCAGAAGG GACCAAAGAT TCTGGAAATC TCAATGAACA CTCCATTGAA
    GTTAAGCCAT TCACTGTTAG TGCAGAAAAC AATGGATCGA AGCCCCCCAC AAGTGGCCAC
    TTAATGGCAG ATAAAATGAC TCTTGGGGTC CAAGAAACAG AGTACAGCAC TGATGGAGGT
    GAGAAGAAGA CTTTAAAGAA GCCCCAACGC TTTTCCTGCT TACTTCCGAA TGTTCAAGAG
    CTGAATGACA TTAAGACCAA AAAGAAGCTT CTTATAACTG GAACTGAGCA GTTCAACCAG
    AAACCAAAGA AAGGAGTGCA GTTTCTTCAG GAAAAAGACC TTTTGGCAAC CCCCATGGAT
    AATGCTGAGG TAGCACAGTG GCTCAGAGAA AATCCAAGGC TGGACAAGAA AATGATTGGG
    GAGTTTGTGA GTGATCGCAA AAATCTGGAC TTACTTGAAA GTTTTGTAGG AACCTTCCAT
    TTCCAAGGCC TGAGAGTTGA TGAAGCTTTA AGGCTTTACC TTGAAGCTTT TCGTTTGCCT
    GGCGAGGCAC CTGTGATTCA GAGGTTACTG GAGGCTTTTA CAGAGCACTG GCGAAAATCC
    AATGGAACTC CTTTTGCACA CAGTGATGCC TGCTTTGCTT TGGCTTATGC AGTTATCATG
    CTGAACACAG ACCAACACAA TCACAATGTC CGCAAGCAAA ATGTGCCAAT GACTTTAGAG
    GAATTCAGGA AAAATTTAAA GGGTGTTAAT GGTGGAAAAG ATTTTGACCA AGATATGCTA
    GAGGATATTT ATCACGCAAT CAAAAATGAA GAAATTGTAA TGCCTGAAGA GCAGACTGGC
    TTAGTAAAAG AGAATTATTT TTGGAATGTT TTGCTGCATC GGGGAGCCAC TCCAGAAGGA
    ATGTTCTTGC ATGTGGATCC TGGAAGTTAT GACCATGACC TCTTTACTAT GACTTGGGGG
    CCAACAATAG CTGCCTTGTC CTATGTGTTT GATAAGAGCA TGGATGAAAC GATTATTCAG
    AAAGCAATAT CTGGTTTTAG GAAATGTGCA ATGATATCTG CTCACTATGG TCTCAGTGAT
    GTTTTTGACA ATCTCATAAT TTCCTTGTGC AAGTTTACAA CACTTAGCAG TGAAGCCGTT
    GAAAATCTTC CCACTGTTTT TGGAAGCAAC CTAAAAGCTC AGATCGCTGC AAAGACTGTG
    TTCCACTTAT CCCACAGACA TGGTGACATC CTTCGAGAGG GATGGAAAAA TATTATGGAC
    TCCATGCTGC AGCTTTTTAG AGCTGAGCTG CTTCCCAAGG CTATGGTTGA GGTTGAAGAT
    TTTGTAGACC CCAATGGAAA AATATCTCTT CAACGTGAGG AAATCCCTGC CAACCGTGGG
    GAATCCACTG TACTAAGTTT TGTCAGCTGG CTGACTCTGA GTGGGACAGA ACAGTCAAGT
    CTAAGAGGAC CTTCTACTGA AAATCAAGAG GCCAAGAAAC TAGCGCTGGA ATGTATCAAG
    TTATGTGATC CAGAGAAACT GATCACAGAG AGCAAGTTTC TTCAACTGGA GTCTTTGCAA
    GAACTAATGA AGGCTCTGAT ATCTGTGACA CCAGATGAAG AAACGTATGA TGAAGAAGAT
    GCTGCATTCT GCCTAGAAAT GTTAATGAGA ATTGTGCTAG AAAACAGAGA TCGTGTTGGC
    TGTGTTTGGC AGGCTGTACG AGATCATCTC TATCGTCTAT GTGTCCATGC TGTAGAGTTC
    TGCTTCCTGG TTGAAAGAGC AGTTGTAGGG CTGCTGCGCT TGGCAATACG CCTTTTACGC
    AGAGAGGAAA TTAGTGGTCA GGTTATCCTC TCCTTGAGAA TTCTTTTAAT GATGAAACCA
    ACTGTGTTGT CTAAAGTGAG TCGCCAAGTT GCTTTTGGTG TGCATGAGCT CTTGAAAACC
    AATGCTGCTA ACATCCATTC CAGCAATGAC TGGTACACAC TATTCTGCCT TTTGGAGTGC
    ATTGGGGCAG GAGTGAAGCC GCCCCCTGCC TTACAGGTGA CGACGAGAGC TGACAATGAC
    ACAGGTGCGC AGTCTGACAG TGAAGTTTCC TCATACCATC CAAGTGAAGT GAATCTTGAT
    CGTGGATACA CCTCAGACTC TGAGGTATAT GCAGAACACA GCAAGCAGTC AAAAATGCAC
    CGCTCTGTGA CTGATGTGGA TATGATGAAC AGTGGCTGGC TGATGGTTGG AAAGGAAGAT
    GTTGACTCTT CTAAAGTTGC TACAGTTATT AACAAACCTG TTGCTAATGC CCCAGTGAAT
    CAATACAGCC TAACCATTGG TTTGGATTTT GGCCCTCACG ATACCAAATC TCTCATGAAG
    AGCGTGGAGT CCCTTTCTTT TATTGTTCGA GATGCTGCAC ATGTCACACC AGAAAACTTT
    GAACTCTGTG TTAAAACAAT CCGTGTCTTT GTAGAGGCAA GTCTAAATGG AGGGTACAAA
    TCTCAGGAGA AAAGGGTAAA GAACCACAAA TATGACAGTA AATCCAACCG CTTCAAAAAG
    AAAACCAAGG AAAAAGAAAA TTCTGTCCGC CGTACACGGG CTTCAAATCA GCGACAATAC
    CGATCTCACA GTGATGATGA GGAAGATGAA AGTGTACCAG CCAGCTATCA CACTGTGTCA
    TTACAGCTTT TGGACCTAAT GCACACACTT CACACAAGAG CTGCCACCAT ATACAGTTCT
    TGGGCAGAGG AACAGCGCAA CCTAGAGACA GCTGCTGAGA AAATTACAGC CGATTCTAAG
    ACACTGTGGT CAAACTGTTG GTGTCCTTTA CTGCAAGGTA TTGCTTGGCT CTGCTGTGAT
    GCTCGGCGCC AGGTCAGAAT GCAGGCTCTC ACCTACCTGC AAAGGGCACT TTTAGTCCAT
    GACTTACAGG CTCTTGATGC ACTTGAATGG GAATCATGCT TTAATAAGGT GCTATTTCCA
    TTGCTGACCA AACTATTGGA AAATATTAGT CCAGCTGATG TTGGCGGGAT GGAGGAGACC
    AGAATGAGAG GCTCCACTCT GCTCTCAAAG GTATTTCTAC AGCATCTGTC TCCTCTTCTT
    TCTCTGTCGA CATTTGCTGC CTTGTGGCTT ACTATTCTTG ATTTCATGGA CAAGTACATG
    CATGCTGGCT CCAGTGACTT ACTGCCTGAG GCAATTCCAG AGTCACTGAA AAATATGCTT
    CTCGTAATGG ACACTGCCGG AATATTCCAC AGTGCAGACT CGCGGACGGG CTACTCTGAT
    TTATGGGAGA TCACGTGGGA AAGGATAGAT TGCTTTTTAC CTCGTCTAAG GGAAGAACTG
    TTTAAACAAA CTGTGATACA AGATCCACCC AATATAGTTA CAACAGAAAC TCCTGTTGAG
    CCAAGACCTC CCCCTCAATC AACTCAGTCA GAGAGACCAG CGGAGCAGGG ACCTTGTGAC
    TCAGAGACTC AAAGTTTAGC AATCTCATCT GCTCCAGTAG TTGGATCAAG TTCTTCCTGC
    ACCCCAGTTA GAGTCAGCCC TGTAACAGAT ATACCTCCAC CTATAACTCA GTCTCCAGTC
    ATCCTGCATC CTCTGACCTC CCCGCTGCAG GTGGGAGTGC CACCCATGTC GCTGCCAATC
    ATCCTCAACC CTGCATTAAT TGAAGCTACC TCTCCAGTTC CGCTCTTGTC CACTCCCCGA
    CCTGCTGATG TATCATCTGT CTCAGAGGTC AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017951362.1
    CDS100..5655
    Translation

    Target ORF information:

    RefSeq Version XM_018095873.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018095873.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGT TGAAAATGAG AGCTGGAGGA
    ATGAGTGACT CCTCAAAATG GAAAAAGCAG AAGAAATCTC CTAGGCAGCC ACGTCATGTT
    ACCAAAGCTA TGGCGGCAGC AGAACCCTCC TCTCAGCAAC CAGTAGTTAA TGGCACCACA
    ATTACTACTA ACCTCACTGG TGAAGTTCCT TTTATTGATG CTTCCTCTCC AGATGCTAAT
    TTTCCCATTT CAGGGGTGAG TTCAGAGGCC ACGTCTGCTG TAGTCAGTCC TACCACAGAT
    AGTGGTGTAG ATATGTCCTC TCAGGCAACA TCTAAAGAAG ACCTTTCTGA ACTGGATCCT
    GCAAGTCCAT CTCTTTCTGC CATTCAGCAA TCAGGAGACA TTTGTCTTTC TGAACAGCAA
    AACCAGGATG ATGATAAGAT TGAAGACCTT ATGGAAAGGC ATACATATTC TGCTTCTGTG
    GAATCCATAC CAGAAGTATT AGATGATAAT GTGTCATTGG CTGATCAATC AGACTCCGCT
    TCAGTCCATG ATATGGATTA TGTAAATCCT AGAGGTGTGA GATTTACCCA ATCTTCACAG
    AAAGAAGGAA CAGTCTTAGT ACCATATGGA CTGCCATGCA TTCGGGAATT ATTTCGTTTT
    CTTATTTCTT TAACCAACCC CCATGATCGA CATAACTCCG AAGTGATGAT ACACATGGGT
    TTACAGTTGA TCACAGTTGC TCTTGAGTCT GCACCTATTG AAAATTATGT TTCGCTTTTA
    GGCCTTGTAA AGGATGAACT TTGTCGACAC TTATTTCAGC TACTGAGCAT TGAGAGACTG
    AACCTATATG CAGCTTCCTT GCGAGCATGT TTTCTTTTGT TTGAGGGTAT GAGGGAGCAT
    TTGAAGTTCC AGCTAGAGAT GTACATAAAG AAGCTCATGG ATATTATTAC TGTGGAGAAC
    CCTAAGATGC CTTATGAGAT GAAAGAAATG GCTCTTGAAG CTATTGTGCA ATTGTGGAGA
    ATCCCGAGCT TTGTTACAGA ATTATACATT AATTACGACT GTGATTATTA TTGCTCCAAC
    CTTTTTGAAG ATCTCACTAA ACTGCTTTCC AAGAATGCCT TTCCAGTTTC TGGACAGCTG
    TACACCACTC ATCTCCTTTC CCTGGAAGCT CTGCTAACTG TCATTGATAG CACAGAATCA
    CACTGCCAGG CTAAAAATCT AAGCAATGTG TCACAGGATA AGAAAGAAAC TGGAAAACCA
    AGTGGAGATC TGTCAGAAGG GACCAAAGAT TCTGGAAATC TCAATGAACA CTCCATTGAA
    GTTAAGCCAT TCACTGTTAG TGCAGAAAAC AATGGATCGA AGCCCCCCAC AAGTGGCCAC
    TTAATGGCAG ATAAAATGAC TCTTGGGGTC CAAGAAACAG AGTACAGCAC TGATGGAGGT
    GAGAAGAAGA CTTTAAAGAA GCCCCAACGC TTTTCCTGCT TACTTCCGAA TGTTCAAGAG
    CTGAATGACA TTAAGACCAA AAAGAAGCTT CTTATAACTG GAACTGAGCA GTTCAACCAG
    AAACCAAAGA AAGGAGTGCA GTTTCTTCAG GAAAAAGACC TTTTGGCAAC CCCCATGGAT
    AATGCTGAGG TAGCACAGTG GCTCAGAGAA AATCCAAGGC TGGACAAGAA AATGATTGGG
    GAGTTTGTGA GTGATCGCAA AAATCTGGAC TTACTTGAAA GTTTTGTAGG AACCTTCCAT
    TTCCAAGGCC TGAGAGTTGA TGAAGCTTTA AGGCTTTACC TTGAAGCTTT TCGTTTGCCT
    GGCGAGGCAC CTGTGATTCA GAGGTTACTG GAGGCTTTTA CAGAGCACTG GCGAAAATCC
    AATGGAACTC CTTTTGCACA CAGTGATGCC TGCTTTGCTT TGGCTTATGC AGTTATCATG
    CTGAACACAG ACCAACACAA TCACAATGTC CGCAAGCAAA ATGTGCCAAT GACTTTAGAG
    GAATTCAGGA AAAATTTAAA GGGTGTTAAT GGTGGAAAAG ATTTTGACCA AGATATGCTA
    GAGGATATTT ATCACGCAAT CAAAAATGAA GAAATTGTAA TGCCTGAAGA GCAGACTGGC
    TTAGTAAAAG AGAATTATTT TTGGAATGTT TTGCTGCATC GGGGAGCCAC TCCAGAAGGA
    ATGTTCTTGC ATGTGGATCC TGGAAGTTAT GACCATGACC TCTTTACTAT GACTTGGGGG
    CCAACAATAG CTGCCTTGTC CTATGTGTTT GATAAGAGCA TGGATGAAAC GATTATTCAG
    AAAGCAATAT CTGGTTTTAG GAAATGTGCA ATGATATCTG CTCACTATGG TCTCAGTGAT
    GTTTTTGACA ATCTCATAAT TTCCTTGTGC AAGTTTACAA CACTTAGCAG TGAAGCCGTT
    GAAAATCTTC CCACTGTTTT TGGAAGCAAC CTAAAAGCTC AGATCGCTGC AAAGACTGTG
    TTCCACTTAT CCCACAGACA TGGTGACATC CTTCGAGAGG GATGGAAAAA TATTATGGAC
    TCCATGCTGC AGCTTTTTAG AGCTGAGCTG CTTCCCAAGG CTATGGTTGA GGTTGAAGAT
    TTTGTAGACC CCAATGGAAA AATATCTCTT CAACGTGAGG AAATCCCTGC CAACCGTGGG
    GAATCCACTG TACTAAGTTT TGTCAGCTGG CTGACTCTGA GTGGGACAGA ACAGTCAAGT
    CTAAGAGGAC CTTCTACTGA AAATCAAGAG GCCAAGAAAC TAGCGCTGGA ATGTATCAAG
    TTATGTGATC CAGAGAAACT GATCACAGAG AGCAAGTTTC TTCAACTGGA GTCTTTGCAA
    GAACTAATGA AGGCTCTGAT ATCTGTGACA CCAGATGAAG AAACGTATGA TGAAGAAGAT
    GCTGCATTCT GCCTAGAAAT GTTAATGAGA ATTGTGCTAG AAAACAGAGA TCGTGTTGGC
    TGTGTTTGGC AGGCTGTACG AGATCATCTC TATCGTCTAT GTGTCCATGC TGTAGAGTTC
    TGCTTCCTGG TTGAAAGAGC AGTTGTAGGG CTGCTGCGCT TGGCAATACG CCTTTTACGC
    AGAGAGGAAA TTAGTGGTCA GGTTATCCTC TCCTTGAGAA TTCTTTTAAT GATGAAACCA
    ACTGTGTTGT CTAAAGTGAG TCGCCAAGTT GCTTTTGGTG TGCATGAGCT CTTGAAAACC
    AATGCTGCTA ACATCCATTC CAGCAATGAC TGGTACACAC TATTCTGCCT TTTGGAGTGC
    ATTGGGGCAG GAGTGAAGCC GCCCCCTGCC TTACAGGTGA CGACGAGAGC TGACAATGAC
    ACAGGTGCGC AGTCTGACAG TGAAGTTTCC TCATACCATC CAAGTGAAGT GAATCTTGAT
    CGTGGATACA CCTCAGACTC TGAGGTATAT GCAGAACACA GCAAGCAGTC AAAAATGCAC
    CGCTCTGTGA CTGATGTGGA TATGATGAAC AGTGGCTGGC TGATGGTTGG AAAGGAAGAT
    GTTGACTCTT CTAAAGTTGC TACAGTTATT AACAAACCTG TTGCTAATGC CCCAGTGAAT
    CAATACAGCC TAACCATTGG TTTGGATTTT GGCCCTCACG ATACCAAATC TCTCATGAAG
    AGCGTGGAGT CCCTTTCTTT TATTGTTCGA GATGCTGCAC ATGTCACACC AGAAAACTTT
    GAACTCTGTG TTAAAACAAT CCGTGTCTTT GTAGAGGCAA GTCTAAATGG AGGGTACAAA
    TCTCAGGAGA AAAGGGTAAA GAACCACAAA TATGACAGTA AATCCAACCG CTTCAAAAAG
    AAAACCAAGG AAAAAGAAAA TTCTGTCCGC CGTACACGGG CTTCAAATCA GCGACAATAC
    CGATCTCACA GTGATGATGA GGAAGATGAA AGTGTACCAG CCAGCTATCA CACTGTGTCA
    TTACAGCTTT TGGACCTAAT GCACACACTT CACACAAGAG CTGCCACCAT ATACAGTTCT
    TGGGCAGAGG AACAGCGCAA CCTAGAGACA GCTGCTGAGA AAATTACAGC CGATTCTAAG
    ACACTGTGGT CAAACTGTTG GTGTCCTTTA CTGCAAGGTA TTGCTTGGCT CTGCTGTGAT
    GCTCGGCGCC AGGTCAGAAT GCAGGCTCTC ACCTACCTGC AAAGGGCACT TTTAGTCCAT
    GACTTACAGG CTCTTGATGC ACTTGAATGG GAATCATGCT TTAATAAGGT GCTATTTCCA
    TTGCTGACCA AACTATTGGA AAATATTAGT CCAGCTGATG TTGGCGGGAT GGAGGAGACC
    AGAATGAGAG GCTCCACTCT GCTCTCAAAG GTATTTCTAC AGCATCTGTC TCCTCTTCTT
    TCTCTGTCGA CATTTGCTGC CTTGTGGCTT ACTATTCTTG ATTTCATGGA CAAGTACATG
    CATGCTGGCT CCAGTGACTT ACTGCCTGAG GCAATTCCAG AGTCACTGAA AAATATGCTT
    CTCGTAATGG ACACTGCCGG AATATTCCAC AGTGCAGACT CGCGGACGGG CTACTCTGAT
    TTATGGGAGA TCACGTGGGA AAGGATAGAT TGCTTTTTAC CTCGTCTAAG GGAAGAACTG
    TTTAAACAAA CTGTGATACA AGATCCACCC AATATAGTTA CAACAGAAAC TCCTGTTGAG
    CCAAGACCTC CCCCTCAATC AACTCAGTCA GAGAGACCAG CGGAGCAGGG ACCTTGTGAC
    TCAGAGACTC AAAGTTTAGC AATCTCATCT GCTCCAGTAG TTGGATCAAG TTCTTCCTGC
    ACCCCAGTTA GAGTCAGCCC TGTAACAGAT ATACCTCCAC CTATAACTCA GTCTCCAGTC
    ATCCTGCATC CTCTGACCTC CCCGCTGCAG GTGGGAGTGC CACCCATGTC GCTGCCAATC
    ATCCTCAACC CTGCATTAAT TGAAGCTACC TCTCCAGTTC CGCTCTTGTC CACTCCCCGA
    CCTGCTGATG TATCATCTGT CTCAGAGGTC AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa62541
    Clone ID Related Accession (Same CDS sequence) XM_018095871.2
    Accession Version XM_018095871.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5568bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018095871.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGT TGAAAATGAG AGCTGGAGGA
    ATGAGTGACT CCTCAAAATG GAAAAAGCAG AAGAAATCTC CTAGGCAGCC ACGTCATGTT
    ACCAAAGCTA TGGCGGCAGC AGAACCCTCC TCTCAGCAAC CAGTAGTTAA TGGCACCACA
    ATTACTACTA ACCTCACTGG TGAAGTTCCT TTTATTGATG CTTCCTCTCC AGATGCTAAT
    TTTCCCATTT CAGGGGTGAG TTCAGAGGCC ACGTCTGCTG TAGTCAGTCC TACCACAGAT
    AGTGGTGTAG ATATGTCCTC TCAGGCAACA TCTAAAGAAG ACCTTTCTGA ACTGGATCCT
    GCAAGTCCAT CTCTTTCTGC CATTCAGCAA TCAGGAGACA TTTGTCTTTC TGAACAGCAA
    AACCAGGATG ATGATAAGAT TGAAGACCTT ATGGAAAGGC ATACATATTC TGCTTCTGTG
    GAATCCATAC CAGAAGTATT AGATGATAAT GTGTCATTGG CTGATCAATC AGACTCCGCT
    TCAGTCCATG ATATGGATTA TGTAAATCCT AGAGGTGTGA GATTTACCCA ATCTTCACAG
    AAAGAAGGAA CAGTCTTAGT ACCATATGGA CTGCCATGCA TTCGGGAATT ATTTCGTTTT
    CTTATTTCTT TAACCAACCC CCATGATCGA CATAACTCCG AAGTGATGAT ACACATGGGT
    TTACAGTTGA TCACAGTTGC TCTTGAGTCT GCACCTATTG AAAATTATGT TTCGCTTTTA
    GGCCTTGTAA AGGATGAACT TTGTCGACAC TTATTTCAGC TACTGAGCAT TGAGAGACTG
    AACCTATATG CAGCTTCCTT GCGAGCATGT TTTCTTTTGT TTGAGGGTAT GAGGGAGCAT
    TTGAAGTTCC AGCTAGAGAT GTACATAAAG AAGCTCATGG ATATTATTAC TGTGGAGAAC
    CCTAAGATGC CTTATGAGAT GAAAGAAATG GCTCTTGAAG CTATTGTGCA ATTGTGGAGA
    ATCCCGAGCT TTGTTACAGA ATTATACATT AATTACGACT GTGATTATTA TTGCTCCAAC
    CTTTTTGAAG ATCTCACTAA ACTGCTTTCC AAGAATGCCT TTCCAGTTTC TGGACAGCTG
    TACACCACTC ATCTCCTTTC CCTGGAAGCT CTGCTAACTG TCATTGATAG CACAGAATCA
    CACTGCCAGG CTAAAAATCT AAGCAATGTG TCACAGGATA AGAAAGAAAC TGGAAAACCA
    AGTGGAGATC TGTCAGAAGG GACCAAAGAT TCTGGAAATC TCAATGAACA CTCCATTGAA
    GTTAAGCCAT TCACTGTTAG TGCAGAAAAC AATGGATCGA AGCCCCCCAC AAGTGGCCAC
    TTAATGGCAG ATAAAATGAC TCTTGGGGTC CAAGAAACAG AGTACAGCAC TGATGGAGGT
    GAGAAGAAGA CTTTAAAGAA GCCCCAACGC TTTTCCTGCT TACTTCCGAA TGTTCAAGAG
    CTGAATGACA TTAAGACCAA AAAGAAGCTT CTTATAACTG GAACTGAGCA GTTCAACCAG
    AAACCAAAGA AAGGAGTGCA GTTTCTTCAG GAAAAAGACC TTTTGGCAAC CCCCATGGAT
    AATGCTGAGG TAGCACAGTG GCTCAGAGAA AATCCAAGGC TGGACAAGAA AATGATTGGG
    GAGTTTGTGA GTGATCGCAA AAATCTGGAC TTACTTGAAA GTTTTGTAGG AACCTTCCAT
    TTCCAAGGCC TGAGAGTTGA TGAAGCTTTA AGGCTTTACC TTGAAGCTTT TCGTTTGCCT
    GGCGAGGCAC CTGTGATTCA GAGGTTACTG GAGGCTTTTA CAGAGCACTG GCGAAAATCC
    AATGGAACTC CTTTTGCACA CAGTGATGCC TGCTTTGCTT TGGCTTATGC AGTTATCATG
    CTGAACACAG ACCAACACAA TCACAATGTC CGCAAGCAAA ATGTGCCAAT GACTTTAGAG
    GAATTCAGGA AAAATTTAAA GGGTGTTAAT GGTGGAAAAG ATTTTGACCA AGATATGCTA
    GAGGATATTT ATCACGCAAT CAAAAATGAA GAAATTGTAA TGCCTGAAGA GCAGACTGGC
    TTAGTAAAAG AGAATTATTT TTGGAATGTT TTGCTGCATC GGGGAGCCAC TCCAGAAGGA
    ATGTTCTTGC ATGTGGATCC TGGAAGTTAT GACCATGACC TCTTTACTAT GACTTGGGGG
    CCAACAATAG CTGCCTTGTC CTATGTGTTT GATAAGAGCA TGGATGAAAC GATTATTCAG
    AAAGCAATAT CTGGTTTTAG GAAATGTGCA ATGATATCTG CTCACTATGG TCTCAGTGAT
    GTTTTTGACA ATCTCATAAT TTCCTTGTGC AAGTTTACAA CACTTAGCAG TGAAGCCGTT
    GAAAATCTTC CCACTGTTTT TGGAAGCAAC CTAAAAGCTC AGATCGCTGC AAAGACTGTG
    TTCCACTTAT CCCACAGACA TGGTGACATC CTTCGAGAGG GATGGAAAAA TATTATGGAC
    TCCATGCTGC AGCTTTTTAG AGCTGAGCTG CTTCCCAAGG CTATGGTTGA GGTTGAAGAT
    TTTGTAGACC CCAATGGAAA AATATCTCTT CAACGTGAGG AAATCCCTGC CAACCGTGGG
    GAATCCACTG TACTAAGTTT TGTCAGCTGG CTGACTCTGA GTGGGACAGA ACAGTCAAGT
    CTAAGAGGAC CTTCTACTGA AAATCAAGAG GCCAAGAAAC TAGCGCTGGA ATGTATCAAG
    TTATGTGATC CAGAGAAACT GATCACAGAG AGCAAGTTTC TTCAACTGGA GTCTTTGCAA
    GAACTAATGA AGGCTCTGAT ATCTGTGACA CCAGATGAAG AAACGTATGA TGAAGAAGAT
    GCTGCATTCT GCCTAGAAAT GTTAATGAGA ATTGTGCTAG AAAACAGAGA TCGTGTTGGC
    TGTGTTTGGC AGGCTGTACG AGATCATCTC TATCGTCTAT GTGTCCATGC TGTAGAGTTC
    TGCTTCCTGG TTGAAAGAGC AGTTGTAGGG CTGCTGCGCT TGGCAATACG CCTTTTACGC
    AGAGAGGAAA TTAGTGGTCA GGTTATCCTC TCCTTGAGAA TTCTTTTAAT GATGAAACCA
    ACTGTGTTGT CTAAAGTGAG TCGCCAAGTT GCTTTTGGTG TGCATGAGCT CTTGAAAACC
    AATGCTGCTA ACATCCATTC CAGCAATGAC TGGTACACAC TATTCTGCCT TTTGGAGTGC
    ATTGGGGCAG GAGTGAAGCC GCCCCCTGCC TTACAGGTGA CGACGAGAGC TGACAATGAC
    ACAGGTGCGC AGTCTGACAG TGAAGTTTCC TCATACCATC CAAGTGAAGT GAATCTTGAT
    CGTGGATACA CCTCAGACTC TGAGGTATAT GCAGAACACA GCAAGCAGTC AAAAATGCAC
    CGCTCTGTGA CTGATGTGGA TATGATGAAC AGTGGCTGGC TGATGGTTGG AAAGGAAGAT
    GTTGACTCTT CTAAAGTTGC TACAGTTATT AACAAACCTG TTGCTAATGC CCCAGTGAAT
    CAATACAGCC TAACCATTGG TTTGGATTTT GGCCCTCACG ATACCAAATC TCTCATGAAG
    AGCGTGGAGT CCCTTTCTTT TATTGTTCGA GATGCTGCAC ATGTCACACC AGAAAACTTT
    GAACTCTGTG TTAAAACAAT CCGTGTCTTT GTAGAGGCAA GTCTAAATGG AGGGTACAAA
    TCTCAGGAGA AAAGGGTAAA GAACCACAAA TATGACAGTA AATCCAACCG CTTCAAAAAG
    AAAACCAAGG AAAAAGAAAA TTCTGTCCGC CGTACACGGG CTTCAAATCA GCGACAATAC
    CGATCTCACA GTGATGATGA GGAAGATGAA AGTGTACCAG CCAGCTATCA CACTGTGTCA
    TTACAGGTTA GTCATGATCT TTTGGACCTA ATGCACACAC TTCACACAAG AGCTGCCACC
    ATATACAGTT CTTGGGCAGA GGAACAGCGC AACCTAGAGA CAGCTGCTGA GAAAATTACA
    GCCGATTCTA AGACACTGTG GTCAAACTGT TGGTGTCCTT TACTGCAAGG TATTGCTTGG
    CTCTGCTGTG ATGCTCGGCG CCAGGTCAGA ATGCAGGCTC TCACCTACCT GCAAAGGGCA
    CTTTTAGTCC ATGACTTACA GGCTCTTGAT GCACTTGAAT GGGAATCATG CTTTAATAAG
    GTGCTATTTC CATTGCTGAC CAAACTATTG GAAAATATTA GTCCAGCTGA TGTTGGCGGG
    ATGGAGGAGA CCAGAATGAG AGGCTCCACT CTGCTCTCAA AGGTATTTCT ACAGCATCTG
    TCTCCTCTTC TTTCTCTGTC GACATTTGCT GCCTTGTGGC TTACTATTCT TGATTTCATG
    GACAAGTACA TGCATGCTGG CTCCAGTGAC TTACTGCCTG AGGCAATTCC AGAGTCACTG
    AAAAATATGC TTCTCGTAAT GGACACTGCC GGAATATTCC ACAGTGCAGA CTCGCGGACG
    GGCTACTCTG ATTTATGGGA GATCACGTGG GAAAGGATAG ATTGCTTTTT ACCTCGTCTA
    AGGGAAGAAC TGTTTAAACA AACTGTGATA CAAGATCCAC CCAATATAGT TACAACAGAA
    ACTCCTGTTG AGCCAAGACC TCCCCCTCAA TCAACTCAGT CAGAGAGACC AGCGGAGCAG
    GGACCTTGTG ACTCAGAGAC TCAAAGTTTA GCAATCTCAT CTGCTCCAGT AGTTGGATCA
    AGTTCTTCCT GCACCCCAGT TAGAGTCAGC CCTGTAACAG ATATACCTCC ACCTATAACT
    CAGTCTCCAG TCATCCTGCA TCCTCTGACC TCCCCGCTGC AGGTGGGAGT GCCACCCATG
    TCGCTGCCAA TCATCCTCAA CCCTGCATTA ATTGAAGCTA CCTCTCCAGT TCCGCTCTTG
    TCCACTCCCC GACCTGCTGA TGTATCATCT GTCTCAGAGG TCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017951360.1
    CDS100..5667
    Translation

    Target ORF information:

    RefSeq Version XM_018095871.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018095871.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGT TGAAAATGAG AGCTGGAGGA
    ATGAGTGACT CCTCAAAATG GAAAAAGCAG AAGAAATCTC CTAGGCAGCC ACGTCATGTT
    ACCAAAGCTA TGGCGGCAGC AGAACCCTCC TCTCAGCAAC CAGTAGTTAA TGGCACCACA
    ATTACTACTA ACCTCACTGG TGAAGTTCCT TTTATTGATG CTTCCTCTCC AGATGCTAAT
    TTTCCCATTT CAGGGGTGAG TTCAGAGGCC ACGTCTGCTG TAGTCAGTCC TACCACAGAT
    AGTGGTGTAG ATATGTCCTC TCAGGCAACA TCTAAAGAAG ACCTTTCTGA ACTGGATCCT
    GCAAGTCCAT CTCTTTCTGC CATTCAGCAA TCAGGAGACA TTTGTCTTTC TGAACAGCAA
    AACCAGGATG ATGATAAGAT TGAAGACCTT ATGGAAAGGC ATACATATTC TGCTTCTGTG
    GAATCCATAC CAGAAGTATT AGATGATAAT GTGTCATTGG CTGATCAATC AGACTCCGCT
    TCAGTCCATG ATATGGATTA TGTAAATCCT AGAGGTGTGA GATTTACCCA ATCTTCACAG
    AAAGAAGGAA CAGTCTTAGT ACCATATGGA CTGCCATGCA TTCGGGAATT ATTTCGTTTT
    CTTATTTCTT TAACCAACCC CCATGATCGA CATAACTCCG AAGTGATGAT ACACATGGGT
    TTACAGTTGA TCACAGTTGC TCTTGAGTCT GCACCTATTG AAAATTATGT TTCGCTTTTA
    GGCCTTGTAA AGGATGAACT TTGTCGACAC TTATTTCAGC TACTGAGCAT TGAGAGACTG
    AACCTATATG CAGCTTCCTT GCGAGCATGT TTTCTTTTGT TTGAGGGTAT GAGGGAGCAT
    TTGAAGTTCC AGCTAGAGAT GTACATAAAG AAGCTCATGG ATATTATTAC TGTGGAGAAC
    CCTAAGATGC CTTATGAGAT GAAAGAAATG GCTCTTGAAG CTATTGTGCA ATTGTGGAGA
    ATCCCGAGCT TTGTTACAGA ATTATACATT AATTACGACT GTGATTATTA TTGCTCCAAC
    CTTTTTGAAG ATCTCACTAA ACTGCTTTCC AAGAATGCCT TTCCAGTTTC TGGACAGCTG
    TACACCACTC ATCTCCTTTC CCTGGAAGCT CTGCTAACTG TCATTGATAG CACAGAATCA
    CACTGCCAGG CTAAAAATCT AAGCAATGTG TCACAGGATA AGAAAGAAAC TGGAAAACCA
    AGTGGAGATC TGTCAGAAGG GACCAAAGAT TCTGGAAATC TCAATGAACA CTCCATTGAA
    GTTAAGCCAT TCACTGTTAG TGCAGAAAAC AATGGATCGA AGCCCCCCAC AAGTGGCCAC
    TTAATGGCAG ATAAAATGAC TCTTGGGGTC CAAGAAACAG AGTACAGCAC TGATGGAGGT
    GAGAAGAAGA CTTTAAAGAA GCCCCAACGC TTTTCCTGCT TACTTCCGAA TGTTCAAGAG
    CTGAATGACA TTAAGACCAA AAAGAAGCTT CTTATAACTG GAACTGAGCA GTTCAACCAG
    AAACCAAAGA AAGGAGTGCA GTTTCTTCAG GAAAAAGACC TTTTGGCAAC CCCCATGGAT
    AATGCTGAGG TAGCACAGTG GCTCAGAGAA AATCCAAGGC TGGACAAGAA AATGATTGGG
    GAGTTTGTGA GTGATCGCAA AAATCTGGAC TTACTTGAAA GTTTTGTAGG AACCTTCCAT
    TTCCAAGGCC TGAGAGTTGA TGAAGCTTTA AGGCTTTACC TTGAAGCTTT TCGTTTGCCT
    GGCGAGGCAC CTGTGATTCA GAGGTTACTG GAGGCTTTTA CAGAGCACTG GCGAAAATCC
    AATGGAACTC CTTTTGCACA CAGTGATGCC TGCTTTGCTT TGGCTTATGC AGTTATCATG
    CTGAACACAG ACCAACACAA TCACAATGTC CGCAAGCAAA ATGTGCCAAT GACTTTAGAG
    GAATTCAGGA AAAATTTAAA GGGTGTTAAT GGTGGAAAAG ATTTTGACCA AGATATGCTA
    GAGGATATTT ATCACGCAAT CAAAAATGAA GAAATTGTAA TGCCTGAAGA GCAGACTGGC
    TTAGTAAAAG AGAATTATTT TTGGAATGTT TTGCTGCATC GGGGAGCCAC TCCAGAAGGA
    ATGTTCTTGC ATGTGGATCC TGGAAGTTAT GACCATGACC TCTTTACTAT GACTTGGGGG
    CCAACAATAG CTGCCTTGTC CTATGTGTTT GATAAGAGCA TGGATGAAAC GATTATTCAG
    AAAGCAATAT CTGGTTTTAG GAAATGTGCA ATGATATCTG CTCACTATGG TCTCAGTGAT
    GTTTTTGACA ATCTCATAAT TTCCTTGTGC AAGTTTACAA CACTTAGCAG TGAAGCCGTT
    GAAAATCTTC CCACTGTTTT TGGAAGCAAC CTAAAAGCTC AGATCGCTGC AAAGACTGTG
    TTCCACTTAT CCCACAGACA TGGTGACATC CTTCGAGAGG GATGGAAAAA TATTATGGAC
    TCCATGCTGC AGCTTTTTAG AGCTGAGCTG CTTCCCAAGG CTATGGTTGA GGTTGAAGAT
    TTTGTAGACC CCAATGGAAA AATATCTCTT CAACGTGAGG AAATCCCTGC CAACCGTGGG
    GAATCCACTG TACTAAGTTT TGTCAGCTGG CTGACTCTGA GTGGGACAGA ACAGTCAAGT
    CTAAGAGGAC CTTCTACTGA AAATCAAGAG GCCAAGAAAC TAGCGCTGGA ATGTATCAAG
    TTATGTGATC CAGAGAAACT GATCACAGAG AGCAAGTTTC TTCAACTGGA GTCTTTGCAA
    GAACTAATGA AGGCTCTGAT ATCTGTGACA CCAGATGAAG AAACGTATGA TGAAGAAGAT
    GCTGCATTCT GCCTAGAAAT GTTAATGAGA ATTGTGCTAG AAAACAGAGA TCGTGTTGGC
    TGTGTTTGGC AGGCTGTACG AGATCATCTC TATCGTCTAT GTGTCCATGC TGTAGAGTTC
    TGCTTCCTGG TTGAAAGAGC AGTTGTAGGG CTGCTGCGCT TGGCAATACG CCTTTTACGC
    AGAGAGGAAA TTAGTGGTCA GGTTATCCTC TCCTTGAGAA TTCTTTTAAT GATGAAACCA
    ACTGTGTTGT CTAAAGTGAG TCGCCAAGTT GCTTTTGGTG TGCATGAGCT CTTGAAAACC
    AATGCTGCTA ACATCCATTC CAGCAATGAC TGGTACACAC TATTCTGCCT TTTGGAGTGC
    ATTGGGGCAG GAGTGAAGCC GCCCCCTGCC TTACAGGTGA CGACGAGAGC TGACAATGAC
    ACAGGTGCGC AGTCTGACAG TGAAGTTTCC TCATACCATC CAAGTGAAGT GAATCTTGAT
    CGTGGATACA CCTCAGACTC TGAGGTATAT GCAGAACACA GCAAGCAGTC AAAAATGCAC
    CGCTCTGTGA CTGATGTGGA TATGATGAAC AGTGGCTGGC TGATGGTTGG AAAGGAAGAT
    GTTGACTCTT CTAAAGTTGC TACAGTTATT AACAAACCTG TTGCTAATGC CCCAGTGAAT
    CAATACAGCC TAACCATTGG TTTGGATTTT GGCCCTCACG ATACCAAATC TCTCATGAAG
    AGCGTGGAGT CCCTTTCTTT TATTGTTCGA GATGCTGCAC ATGTCACACC AGAAAACTTT
    GAACTCTGTG TTAAAACAAT CCGTGTCTTT GTAGAGGCAA GTCTAAATGG AGGGTACAAA
    TCTCAGGAGA AAAGGGTAAA GAACCACAAA TATGACAGTA AATCCAACCG CTTCAAAAAG
    AAAACCAAGG AAAAAGAAAA TTCTGTCCGC CGTACACGGG CTTCAAATCA GCGACAATAC
    CGATCTCACA GTGATGATGA GGAAGATGAA AGTGTACCAG CCAGCTATCA CACTGTGTCA
    TTACAGGTTA GTCATGATCT TTTGGACCTA ATGCACACAC TTCACACAAG AGCTGCCACC
    ATATACAGTT CTTGGGCAGA GGAACAGCGC AACCTAGAGA CAGCTGCTGA GAAAATTACA
    GCCGATTCTA AGACACTGTG GTCAAACTGT TGGTGTCCTT TACTGCAAGG TATTGCTTGG
    CTCTGCTGTG ATGCTCGGCG CCAGGTCAGA ATGCAGGCTC TCACCTACCT GCAAAGGGCA
    CTTTTAGTCC ATGACTTACA GGCTCTTGAT GCACTTGAAT GGGAATCATG CTTTAATAAG
    GTGCTATTTC CATTGCTGAC CAAACTATTG GAAAATATTA GTCCAGCTGA TGTTGGCGGG
    ATGGAGGAGA CCAGAATGAG AGGCTCCACT CTGCTCTCAA AGGTATTTCT ACAGCATCTG
    TCTCCTCTTC TTTCTCTGTC GACATTTGCT GCCTTGTGGC TTACTATTCT TGATTTCATG
    GACAAGTACA TGCATGCTGG CTCCAGTGAC TTACTGCCTG AGGCAATTCC AGAGTCACTG
    AAAAATATGC TTCTCGTAAT GGACACTGCC GGAATATTCC ACAGTGCAGA CTCGCGGACG
    GGCTACTCTG ATTTATGGGA GATCACGTGG GAAAGGATAG ATTGCTTTTT ACCTCGTCTA
    AGGGAAGAAC TGTTTAAACA AACTGTGATA CAAGATCCAC CCAATATAGT TACAACAGAA
    ACTCCTGTTG AGCCAAGACC TCCCCCTCAA TCAACTCAGT CAGAGAGACC AGCGGAGCAG
    GGACCTTGTG ACTCAGAGAC TCAAAGTTTA GCAATCTCAT CTGCTCCAGT AGTTGGATCA
    AGTTCTTCCT GCACCCCAGT TAGAGTCAGC CCTGTAACAG ATATACCTCC ACCTATAACT
    CAGTCTCCAG TCATCCTGCA TCCTCTGACC TCCCCGCTGC AGGTGGGAGT GCCACCCATG
    TCGCTGCCAA TCATCCTCAA CCCTGCATTA ATTGAAGCTA CCTCTCCAGT TCCGCTCTTG
    TCCACTCCCC GACCTGCTGA TGTATCATCT GTCTCAGAGG TCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa12833
    Clone ID Related Accession (Same CDS sequence) XM_018095871.1
    Accession Version XM_018095871.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5568bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGT TGAAAATGAG AGCTGGAGGA
    ATGAGTGACT CCTCAAAATG GAAAAAGCAG AAGAAATCTC CTAGGCAGCC ACGTCATGTT
    ACCAAAGCTA TGGCGGCAGC AGAACCCTCC TCTCAGCAAC CAGTAGTTAA TGGCACCACA
    ATTACTACTA ACCTCACTGG TGAAGTTCCT TTTATTGATG CTTCCTCTCC AGATGCTAAT
    TTTCCCATTT CAGGGGTGAG TTCAGAGGCC ACGTCTGCTG TAGTCAGTCC TACCACAGAT
    AGTGGTGTAG ATATGTCCTC TCAGGCAACA TCTAAAGAAG ACCTTTCTGA ACTGGATCCT
    GCAAGTCCAT CTCTTTCTGC CATTCAGCAA TCAGGAGACA TTTGTCTTTC TGAACAGCAA
    AACCAGGATG ATGATAAGAT TGAAGACCTT ATGGAAAGGC ATACATATTC TGCTTCTGTG
    GAATCCATAC CAGAAGTATT AGATGATAAT GTGTCATTGG CTGATCAATC AGACTCCGCT
    TCAGTCCATG ATATGGATTA TGTAAATCCT AGAGGTGTGA GATTTACCCA ATCTTCACAG
    AAAGAAGGAA CAGTCTTAGT ACCATATGGA CTGCCATGCA TTCGGGAATT ATTTCGTTTT
    CTTATTTCTT TAACCAACCC CCATGATCGA CATAACTCCG AAGTGATGAT ACACATGGGT
    TTACAGTTGA TCACAGTTGC TCTTGAGTCT GCACCTATTG AAAATTATGT TTCGCTTTTA
    GGCCTTGTAA AGGATGAACT TTGTCGACAC TTATTTCAGC TACTGAGCAT TGAGAGACTG
    AACCTATATG CAGCTTCCTT GCGAGCATGT TTTCTTTTGT TTGAGGGTAT GAGGGAGCAT
    TTGAAGTTCC AGCTAGAGAT GTACATAAAG AAGCTCATGG ATATTATTAC TGTGGAGAAC
    CCTAAGATGC CTTATGAGAT GAAAGAAATG GCTCTTGAAG CTATTGTGCA ATTGTGGAGA
    ATCCCGAGCT TTGTTACAGA ATTATACATT AATTACGACT GTGATTATTA TTGCTCCAAC
    CTTTTTGAAG ATCTCACTAA ACTGCTTTCC AAGAATGCCT TTCCAGTTTC TGGACAGCTG
    TACACCACTC ATCTCCTTTC CCTGGAAGCT CTGCTAACTG TCATTGATAG CACAGAATCA
    CACTGCCAGG CTAAAAATCT AAGCAATGTG TCACAGGATA AGAAAGAAAC TGGAAAACCA
    AGTGGAGATC TGTCAGAAGG GACCAAAGAT TCTGGAAATC TCAATGAACA CTCCATTGAA
    GTTAAGCCAT TCACTGTTAG TGCAGAAAAC AATGGATCGA AGCCCCCCAC AAGTGGCCAC
    TTAATGGCAG ATAAAATGAC TCTTGGGGTC CAAGAAACAG AGTACAGCAC TGATGGAGGT
    GAGAAGAAGA CTTTAAAGAA GCCCCAACGC TTTTCCTGCT TACTTCCTAA TGTTCAAGAG
    CTGAATGACA TTAAGACCAA AAAGAAGCTT CTTATAACTG GAACTGAGCA GTTCAACCAG
    AAACCAAAGA AAGGAGTGCA GTTTCTTCAG GAAAAAGACC TTTTGGCAAC CCCCATGGAT
    AATGCTGAGG TAGCACAGTG GCTCAGAGAA AATCCAAGGC TGGACAAGAA AATGATTGGG
    GAGTTTGTGA GTGATCGCAA AAATCTGGAC TTACTTGAAA GTTTTGTAGG AACCTTCCAT
    TTCCAAGGCC TGAGAGTTGA TGAAGCTTTA AGGCTTTACC TTGAAGCTTT TCGTTTGCCT
    GGCGAGGCAC CTGTGATTCA GAGGTTACTG GAGGCTTTTA CAGAGCACTG GCGAAAATCC
    AATGGAACTC CTTTTGCACA CAGTGATGCC TGCTTTGCTT TGGCTTATGC AGTTATCATG
    CTGAACACAG ACCAACACAA TCACAATGTC CGCAAGCAAA ATGTGCCAAT GACTTTAGAG
    GAATTCAGGA AAAATTTAAA GGGTGTTAAT GGTGGAAAAG ATTTTGACCA AGATATGCTA
    GAGGATATTT ATCACGCAAT CAAAAATGAA GAAATTGTAA TGCCTGAAGA GCAGACTGGC
    TTAGTAAAAG AGAATTATTT TTGGAATGTT TTGCTGCATC GGGGAGCCAC TCCAGAAGGA
    ATGTTCTTGC ATGTGGATCC TGGAAGTTAT GACCATGACC TCTTTACTAT GACTTGGGGG
    CCAACAATAG CTGCCTTGTC CTATGTGTTT GATAAGAGCA TGGATGAAAC GATTATTCAG
    AAAGCAATAT CTGGTTTTAG GAAATGTGCA ATGATATCTG CTCACTATGG TCTCAGTGAT
    GTTTTTGACA ATCTCATAAT TTCCTTGTGC AAGTTTACAA CACTTAGCAG TGAAGCCGTT
    GAAAATCTTC CCACTGTTTT TGGAAGCAAC CTAAAAGCTC AGATTGCTGC AAAGACTGTG
    TTCCACTTAT CCCACAGACA TGGTGACATC CTTCGAGAGG GATGGAAAAA TATTATGGAC
    TCCATGCTGC AGCTTTTTAG AGCTGAGCTG CTTCCCAAGG CTATGGTTGA GGTTGAAGAT
    TTTGTAGACC CCAATGGAAA AATATCTCTT CAACGTGAGG AAATCCCTGC CAACCGTGGG
    GAATCCACTG TACTAAGTTT TGTCAGCTGG CTGACTCTGA GTGGGACAGA ACAGTCAAGT
    CTAAGAGGAC CTTCTACTGA AAATCAAGAG GCCAAGAAAC TAGCGCTGGA ATGTATCAAG
    TTATGTGATC CAGAGAAACT GATCACAGAG AGCAAGTTTC TTCAACTGGA GTCTTTGCAA
    GAACTAATGA AGGCTCTGAT ATCTGTGACA CCAGATGAAG AAACGTATGA TGAAGAAGAT
    GCTGCATTCT GCCTAGAAAT GTTAATGAGA ATTGTGCTAG AAAACAGAGA TCGTGTTGGC
    TGTGTTTGGC AGGCTGTACG AGATCATCTC TATCGTCTAT GTGTCCATGC TGTAGAGTTC
    TGCTTCCTGG TTGAAAGAGC AGTTGTAGGG CTGCTGCGCT TGGCAATACG CCTTTTACGC
    AGAGAGGAAA TTAGTGGTCA GGTTATCCTC TCCTTGAGAA TTCTTTTAAT GATGAAACCA
    ACTGTGTTGT CTAAAGTGAG TCGCCAAGTT GCTTTTGGTG TGCATGAGCT CTTGAAAACC
    AATGCTGCTA ACATCCATTC CAGCAATGAC TGGTACACAC TATTCTGCCT TTTGGAGTGC
    ATTGGGGCAG GAGTGAAGCC GCCCCCTGCC TTACAGGTGA CGACGAGAGC TGACAATGAC
    ACAGGTGCGC AGTCTGACAG TGAAGTTTCC TCATACCATC CAAGTGAAGT GAATCTTGAT
    CGTGGATACA CCTCAGACTC TGAGGTATAT GCAGAACACA GCAAGCAGTC AAAAATGCAC
    CGCTCTGTGA CTGATGTGGA TATGATGAAC AGTGGCTGGC TGATGGTTGG AAAGGAAGAT
    GTTGACTCTT CTAAAGTTGC TACAGTTATT AACAAACCTG TTGCTAATGC CCCAGTGAAT
    CAATACAGCC TAACCATTGG TTTGGATTTT GGCCCTCACG ATACCAAATC TCTCATGAAG
    AGCGTGGAGT CCCTTTCTTT TATTGTTCGA GATGCTGCAC ATGTCACACC AGAAAACTTT
    GAACTCTGTG TTAAAACAAT CCGTGTCTTT GTAGAGGCAA GTCTAAATGG AGGGTACAAA
    TCTCAGGAGA AAAGGGTAAA GAACCACAAA TATGACAGTA AATCCAACCG CTTCAAAAAG
    AAAACCAAGG AAAAAGAAAA TTCTGTCCGC CGTACACGGG CTTCAAATCA GCGACAATAC
    CGATCTCACA GTGATGATGA GGAAGATGAA AGTGTACCAG CCAGCTATCA CACTGTGTCA
    TTACAGGTTA GTCATGATCT TTTGGACCTA ATGCACACAC TTCACACAAG AGCTGCCACC
    ATATACAGTT CTTGGGCAGA GGAACAGCGC AACCTAGAGA CAGCTGCTGA GAAAATTACA
    GCCGATTCTA AGACACTGTG GTCAAACTGT TGGTGTCCTT TACTGCAAGG TATTGCTTGG
    CTCTGCTGTG ATGCTCGGCG CCAGGTCAGA ATGCAGGCTC TCACCTACCT GCAAAGGGCA
    CTTTTAGTCC ATGACTTACA GGCTCTTGAT GCACTTGAAT GGGAATCATG CTTTAATAAG
    GTGCTATTTC CATTGCTGAC CAAACTATTG GAAAATATTA GTCCAGCTGA TGTTGGCGGG
    ATGGAGGAGA CCAGAATGAG AGGCTCCACT CTGCTCTCAA AGGTATTTCT ACAGCATCTG
    TCTCCTCTTC TTTCTCTGTC GACATTTGCT GCCTTGTGGC TTACTATTCT TGATTTCATG
    GACAAGTACA TGCATGCTGG CTCCAGTGAC TTACTGCCTG AGGCAATTCC AGAGTCACTG
    AAAAATATGC TTCTCGTAAT GGACACTGCC GGAATATTCC ACAGTGCAGA CTCGCGGACG
    GGCTACTCTG ATTTATGGGA GATCACGTGG GAAAGGATAG ATTGCTTTTT ACCTCGTCTA
    AGGGAAGAAC TGTTTAAACA AACTGTGATA CAAGATCCAC CCAATATAGT TACAACAGAA
    ACTCCTGTTG AGCCAAGACC TCCCCCTCAA TCAACTCAGT CAGAGAGACC AGCGGAGCAG
    GGACCTTGTG ACTCAGAGAC TCAAAGTTTA GCAATCTCAT CTGCTCCAGT AGTTGGATCA
    AGTTCTTCCT GCACCCCAGT TAGAGTCAGC CCTGTAACAG ATATACCTCC ACCTATAACT
    CAGTCTCCAG TCATCCTGCA TCCTCTGACC TCCCCGCTGC AGGTGGGAGT GCCACCCATG
    TCGCTGCCAA TCATCCTCAA CCCTGCATTA ATTGAAGCTA CCTCTCCAGT TCCGCTCTTG
    TCCACTCCCC GACCTGCTGA TGTATCATCT GTCTCAGAGG TCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017951360.1
    CDS163..5730
    Translation

    Target ORF information:

    RefSeq Version XM_018095871.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018095871.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGT TGAAAATGAG AGCTGGAGGA
    ATGAGTGACT CCTCAAAATG GAAAAAGCAG AAGAAATCTC CTAGGCAGCC ACGTCATGTT
    ACCAAAGCTA TGGCGGCAGC AGAACCCTCC TCTCAGCAAC CAGTAGTTAA TGGCACCACA
    ATTACTACTA ACCTCACTGG TGAAGTTCCT TTTATTGATG CTTCCTCTCC AGATGCTAAT
    TTTCCCATTT CAGGGGTGAG TTCAGAGGCC ACGTCTGCTG TAGTCAGTCC TACCACAGAT
    AGTGGTGTAG ATATGTCCTC TCAGGCAACA TCTAAAGAAG ACCTTTCTGA ACTGGATCCT
    GCAAGTCCAT CTCTTTCTGC CATTCAGCAA TCAGGAGACA TTTGTCTTTC TGAACAGCAA
    AACCAGGATG ATGATAAGAT TGAAGACCTT ATGGAAAGGC ATACATATTC TGCTTCTGTG
    GAATCCATAC CAGAAGTATT AGATGATAAT GTGTCATTGG CTGATCAATC AGACTCCGCT
    TCAGTCCATG ATATGGATTA TGTAAATCCT AGAGGTGTGA GATTTACCCA ATCTTCACAG
    AAAGAAGGAA CAGTCTTAGT ACCATATGGA CTGCCATGCA TTCGGGAATT ATTTCGTTTT
    CTTATTTCTT TAACCAACCC CCATGATCGA CATAACTCCG AAGTGATGAT ACACATGGGT
    TTACAGTTGA TCACAGTTGC TCTTGAGTCT GCACCTATTG AAAATTATGT TTCGCTTTTA
    GGCCTTGTAA AGGATGAACT TTGTCGACAC TTATTTCAGC TACTGAGCAT TGAGAGACTG
    AACCTATATG CAGCTTCCTT GCGAGCATGT TTTCTTTTGT TTGAGGGTAT GAGGGAGCAT
    TTGAAGTTCC AGCTAGAGAT GTACATAAAG AAGCTCATGG ATATTATTAC TGTGGAGAAC
    CCTAAGATGC CTTATGAGAT GAAAGAAATG GCTCTTGAAG CTATTGTGCA ATTGTGGAGA
    ATCCCGAGCT TTGTTACAGA ATTATACATT AATTACGACT GTGATTATTA TTGCTCCAAC
    CTTTTTGAAG ATCTCACTAA ACTGCTTTCC AAGAATGCCT TTCCAGTTTC TGGACAGCTG
    TACACCACTC ATCTCCTTTC CCTGGAAGCT CTGCTAACTG TCATTGATAG CACAGAATCA
    CACTGCCAGG CTAAAAATCT AAGCAATGTG TCACAGGATA AGAAAGAAAC TGGAAAACCA
    AGTGGAGATC TGTCAGAAGG GACCAAAGAT TCTGGAAATC TCAATGAACA CTCCATTGAA
    GTTAAGCCAT TCACTGTTAG TGCAGAAAAC AATGGATCGA AGCCCCCCAC AAGTGGCCAC
    TTAATGGCAG ATAAAATGAC TCTTGGGGTC CAAGAAACAG AGTACAGCAC TGATGGAGGT
    GAGAAGAAGA CTTTAAAGAA GCCCCAACGC TTTTCCTGCT TACTTCCTAA TGTTCAAGAG
    CTGAATGACA TTAAGACCAA AAAGAAGCTT CTTATAACTG GAACTGAGCA GTTCAACCAG
    AAACCAAAGA AAGGAGTGCA GTTTCTTCAG GAAAAAGACC TTTTGGCAAC CCCCATGGAT
    AATGCTGAGG TAGCACAGTG GCTCAGAGAA AATCCAAGGC TGGACAAGAA AATGATTGGG
    GAGTTTGTGA GTGATCGCAA AAATCTGGAC TTACTTGAAA GTTTTGTAGG AACCTTCCAT
    TTCCAAGGCC TGAGAGTTGA TGAAGCTTTA AGGCTTTACC TTGAAGCTTT TCGTTTGCCT
    GGCGAGGCAC CTGTGATTCA GAGGTTACTG GAGGCTTTTA CAGAGCACTG GCGAAAATCC
    AATGGAACTC CTTTTGCACA CAGTGATGCC TGCTTTGCTT TGGCTTATGC AGTTATCATG
    CTGAACACAG ACCAACACAA TCACAATGTC CGCAAGCAAA ATGTGCCAAT GACTTTAGAG
    GAATTCAGGA AAAATTTAAA GGGTGTTAAT GGTGGAAAAG ATTTTGACCA AGATATGCTA
    GAGGATATTT ATCACGCAAT CAAAAATGAA GAAATTGTAA TGCCTGAAGA GCAGACTGGC
    TTAGTAAAAG AGAATTATTT TTGGAATGTT TTGCTGCATC GGGGAGCCAC TCCAGAAGGA
    ATGTTCTTGC ATGTGGATCC TGGAAGTTAT GACCATGACC TCTTTACTAT GACTTGGGGG
    CCAACAATAG CTGCCTTGTC CTATGTGTTT GATAAGAGCA TGGATGAAAC GATTATTCAG
    AAAGCAATAT CTGGTTTTAG GAAATGTGCA ATGATATCTG CTCACTATGG TCTCAGTGAT
    GTTTTTGACA ATCTCATAAT TTCCTTGTGC AAGTTTACAA CACTTAGCAG TGAAGCCGTT
    GAAAATCTTC CCACTGTTTT TGGAAGCAAC CTAAAAGCTC AGATTGCTGC AAAGACTGTG
    TTCCACTTAT CCCACAGACA TGGTGACATC CTTCGAGAGG GATGGAAAAA TATTATGGAC
    TCCATGCTGC AGCTTTTTAG AGCTGAGCTG CTTCCCAAGG CTATGGTTGA GGTTGAAGAT
    TTTGTAGACC CCAATGGAAA AATATCTCTT CAACGTGAGG AAATCCCTGC CAACCGTGGG
    GAATCCACTG TACTAAGTTT TGTCAGCTGG CTGACTCTGA GTGGGACAGA ACAGTCAAGT
    CTAAGAGGAC CTTCTACTGA AAATCAAGAG GCCAAGAAAC TAGCGCTGGA ATGTATCAAG
    TTATGTGATC CAGAGAAACT GATCACAGAG AGCAAGTTTC TTCAACTGGA GTCTTTGCAA
    GAACTAATGA AGGCTCTGAT ATCTGTGACA CCAGATGAAG AAACGTATGA TGAAGAAGAT
    GCTGCATTCT GCCTAGAAAT GTTAATGAGA ATTGTGCTAG AAAACAGAGA TCGTGTTGGC
    TGTGTTTGGC AGGCTGTACG AGATCATCTC TATCGTCTAT GTGTCCATGC TGTAGAGTTC
    TGCTTCCTGG TTGAAAGAGC AGTTGTAGGG CTGCTGCGCT TGGCAATACG CCTTTTACGC
    AGAGAGGAAA TTAGTGGTCA GGTTATCCTC TCCTTGAGAA TTCTTTTAAT GATGAAACCA
    ACTGTGTTGT CTAAAGTGAG TCGCCAAGTT GCTTTTGGTG TGCATGAGCT CTTGAAAACC
    AATGCTGCTA ACATCCATTC CAGCAATGAC TGGTACACAC TATTCTGCCT TTTGGAGTGC
    ATTGGGGCAG GAGTGAAGCC GCCCCCTGCC TTACAGGTGA CGACGAGAGC TGACAATGAC
    ACAGGTGCGC AGTCTGACAG TGAAGTTTCC TCATACCATC CAAGTGAAGT GAATCTTGAT
    CGTGGATACA CCTCAGACTC TGAGGTATAT GCAGAACACA GCAAGCAGTC AAAAATGCAC
    CGCTCTGTGA CTGATGTGGA TATGATGAAC AGTGGCTGGC TGATGGTTGG AAAGGAAGAT
    GTTGACTCTT CTAAAGTTGC TACAGTTATT AACAAACCTG TTGCTAATGC CCCAGTGAAT
    CAATACAGCC TAACCATTGG TTTGGATTTT GGCCCTCACG ATACCAAATC TCTCATGAAG
    AGCGTGGAGT CCCTTTCTTT TATTGTTCGA GATGCTGCAC ATGTCACACC AGAAAACTTT
    GAACTCTGTG TTAAAACAAT CCGTGTCTTT GTAGAGGCAA GTCTAAATGG AGGGTACAAA
    TCTCAGGAGA AAAGGGTAAA GAACCACAAA TATGACAGTA AATCCAACCG CTTCAAAAAG
    AAAACCAAGG AAAAAGAAAA TTCTGTCCGC CGTACACGGG CTTCAAATCA GCGACAATAC
    CGATCTCACA GTGATGATGA GGAAGATGAA AGTGTACCAG CCAGCTATCA CACTGTGTCA
    TTACAGGTTA GTCATGATCT TTTGGACCTA ATGCACACAC TTCACACAAG AGCTGCCACC
    ATATACAGTT CTTGGGCAGA GGAACAGCGC AACCTAGAGA CAGCTGCTGA GAAAATTACA
    GCCGATTCTA AGACACTGTG GTCAAACTGT TGGTGTCCTT TACTGCAAGG TATTGCTTGG
    CTCTGCTGTG ATGCTCGGCG CCAGGTCAGA ATGCAGGCTC TCACCTACCT GCAAAGGGCA
    CTTTTAGTCC ATGACTTACA GGCTCTTGAT GCACTTGAAT GGGAATCATG CTTTAATAAG
    GTGCTATTTC CATTGCTGAC CAAACTATTG GAAAATATTA GTCCAGCTGA TGTTGGCGGG
    ATGGAGGAGA CCAGAATGAG AGGCTCCACT CTGCTCTCAA AGGTATTTCT ACAGCATCTG
    TCTCCTCTTC TTTCTCTGTC GACATTTGCT GCCTTGTGGC TTACTATTCT TGATTTCATG
    GACAAGTACA TGCATGCTGG CTCCAGTGAC TTACTGCCTG AGGCAATTCC AGAGTCACTG
    AAAAATATGC TTCTCGTAAT GGACACTGCC GGAATATTCC ACAGTGCAGA CTCGCGGACG
    GGCTACTCTG ATTTATGGGA GATCACGTGG GAAAGGATAG ATTGCTTTTT ACCTCGTCTA
    AGGGAAGAAC TGTTTAAACA AACTGTGATA CAAGATCCAC CCAATATAGT TACAACAGAA
    ACTCCTGTTG AGCCAAGACC TCCCCCTCAA TCAACTCAGT CAGAGAGACC AGCGGAGCAG
    GGACCTTGTG ACTCAGAGAC TCAAAGTTTA GCAATCTCAT CTGCTCCAGT AGTTGGATCA
    AGTTCTTCCT GCACCCCAGT TAGAGTCAGC CCTGTAACAG ATATACCTCC ACCTATAACT
    CAGTCTCCAG TCATCCTGCA TCCTCTGACC TCCCCGCTGC AGGTGGGAGT GCCACCCATG
    TCGCTGCCAA TCATCCTCAA CCCTGCATTA ATTGAAGCTA CCTCTCCAGT TCCGCTCTTG
    TCCACTCCCC GACCTGCTGA TGTATCATCT GTCTCAGAGG TCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa12834
    Clone ID Related Accession (Same CDS sequence) XM_018095872.1
    Accession Version XM_018095872.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5562bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGT TGAAAATGAG AGCTGGAGGA
    ATGAGTGACT CCTCAAAATG GAAAAAGCAG AAGAAATCTC CTAGGCAGCC ACGTCATGTT
    ACCAAAGCTA TGGCGGCAGC AGAACCCTCC TCTCAGCAAC CAGTAGTTAA TGGCACCACA
    ATTACTACTA ACCTCACTGG TGAAGTTCCT TTTATTGATG CTTCCTCTCC AGATGCTAAT
    TTTCCCATTT CAGGGGTGAG TTCAGAGGCC ACGTCTGCTG TAGTCAGTCC TACCACAGAT
    AGTGGTGTAG ATATGTCCTC TCAGGCAACA TCTAAAGAAG ACCTTTCTGA ACTGGATCCT
    GCAAGTCCAT CTCTTTCTGC CATTCAGCAA TCAGGAGACA TTTGTCTTTC TGAACAGCAA
    AACCAGGATG ATGATAAGAT TGAAGACCTT ATGGAAAGGC ATACATATTC TGCTTCTGTG
    GAATCCATAC CAGAAGTATT AGATGATAAT GTGTCATTGG CTGATCAATC AGACTCCGCT
    TCAGTCCATG ATATGGATTA TGTAAATCCT AGAGGTGTGA GATTTACCCA ATCTTCACAG
    AAAGAAGGAA CAGTCTTAGT ACCATATGGA CTGCCATGCA TTCGGGAATT ATTTCGTTTT
    CTTATTTCTT TAACCAACCC CCATGATCGA CATAACTCCG AAGTGATGAT ACACATGGGT
    TTACAGTTGA TCACAGTTGC TCTTGAGTCT GCACCTATTG AAAATTATGT TTCGCTTTTA
    GGCCTTGTAA AGGATGAACT TTGTCGACAC TTATTTCAGC TACTGAGCAT TGAGAGACTG
    AACCTATATG CAGCTTCCTT GCGAGCATGT TTTCTTTTGT TTGAGGGTAT GAGGGAGCAT
    TTGAAGTTCC AGCTAGAGAT GTACATAAAG AAGCTCATGG ATATTATTAC TGTGGAGAAC
    CCTAAGATGC CTTATGAGAT GAAAGAAATG GCTCTTGAAG CTATTGTGCA ATTGTGGAGA
    ATCCCGAGCT TTGTTACAGA ATTATACATT AATTACGACT GTGATTATTA TTGCTCCAAC
    CTTTTTGAAG ATCTCACTAA ACTGCTTTCC AAGAATGCCT TTCCAGTTTC TGGACAGCTG
    TACACCACTC ATCTCCTTTC CCTGGAAGCT CTGCTAACTG TCATTGATAG CACAGAATCA
    CACTGCCAGG CTAAAAATCT AAGCAATGTG TCACAGGATA AGAAAGAAAC TGGAAAACCA
    AGTGGAGATC TGTCAGAAGG GACCAAAGAT TCTGGAAATC TCAATGAACA CTCCATTGAA
    GTTAAGCCAT TCACTGTTAG TGCAGAAAAC AATGGATCGA AGCCCCCCAC AAGTGGCCAC
    TTAATGGCAG ATAAAATGAC TCTTGGGGTC CAAGAAACAG AGTACAGCAC TGATGGAGGT
    GAGAAGAAGA CTTTAAAGAA GCCCCAACGC TTTTCCTGCT TACTTCCTAA TGTTCAAGAG
    CTGAATGACA TTAAGACCAA AAAGAAGCTT CTTATAACTG GAACTGAGCA GTTCAACCAG
    AAACCAAAGA AAGGAGTGCA GTTTCTTCAG GAAAAAGACC TTTTGGCAAC CCCCATGGAT
    AATGCTGAGG TAGCACAGTG GCTCAGAGAA AATCCAAGGC TGGACAAGAA AATGATTGGG
    GAGTTTGTGA GTGATCGCAA AAATCTGGAC TTACTTGAAA GTTTTGTAGG AACCTTCCAT
    TTCCAAGGCC TGAGAGTTGA TGAAGCTTTA AGGCTTTACC TTGAAGCTTT TCGTTTGCCT
    GGCGAGGCAC CTGTGATTCA GAGGTTACTG GAGGCTTTTA CAGAGCACTG GCGAAAATCC
    AATGGAACTC CTTTTGCACA CAGTGATGCC TGCTTTGCTT TGGCTTATGC AGTTATCATG
    CTGAACACAG ACCAACACAA TCACAATGTC CGCAAGCAAA ATGTGCCAAT GACTTTAGAG
    GAATTCAGGA AAAATTTAAA GGGTGTTAAT GGTGGAAAAG ATTTTGACCA AGATATGCTA
    GAGGATATTT ATCACGCAAT CAAAAATGAA GAAATTGTAA TGCCTGAAGA GCAGACTGGC
    TTAGTAAAAG AGAATTATTT TTGGAATGTT TTGCTGCATC GGGGAGCCAC TCCAGAAGGA
    ATGTTCTTGC ATGTGGATCC TGGAAGTTAT GACCATGACC TCTTTACTAT GACTTGGGGG
    CCAACAATAG CTGCCTTGTC CTATGTGTTT GATAAGAGCA TGGATGAAAC GATTATTCAG
    AAAGCAATAT CTGGTTTTAG GAAATGTGCA ATGATATCTG CTCACTATGG TCTCAGTGAT
    GTTTTTGACA ATCTCATAAT TTCCTTGTGC AAGTTTACAA CACTTAGCAG TGAAGCCGTT
    GAAAATCTTC CCACTGTTTT TGGAAGCAAC CTAAAAGCTC AGATTGCTGC AAAGACTGTG
    TTCCACTTAT CCCACAGACA TGGTGACATC CTTCGAGAGG GATGGAAAAA TATTATGGAC
    TCCATGCTGC AGCTTTTTAG AGCTGAGCTG CTTCCCAAGG CTATGGTTGA GGTTGAAGAT
    TTTGTAGACC CCAATGGAAA AATATCTCTT CAACGTGAGG AAATCCCTGC CAACCGTGGG
    GAATCCACTG TACTAAGTTT TGTCAGCTGG CTGACTCTGA GTGGGACAGA ACAGTCAAGT
    CTAAGAGGAC CTTCTACTGA AAATCAAGAG GCCAAGAAAC TAGCGCTGGA ATGTATCAAG
    TTATGTGATC CAGAGAAACT GATCACAGAG AGCAAGTTTC TTCAACTGGA GTCTTTGCAA
    GAACTAATGA AGGCTCTGAT ATCTGTGACA CCAGATGAAG AAACGTATGA TGAAGAAGAT
    GCTGCATTCT GCCTAGAAAT GTTAATGAGA ATTGTGCTAG AAAACAGAGA TCGTGTTGGC
    TGTGTTTGGC AGGCTGTACG AGATCATCTC TATCGTCTAT GTGTCCATGC TGTAGAGTTC
    TGCTTCCTGG TTGAAAGAGC AGTTGTAGGG CTGCTGCGCT TGGCAATACG CCTTTTACGC
    AGAGAGGAAA TTAGTGGTCA GGTTATCCTC TCCTTGAGAA TTCTTTTAAT GATGAAACCA
    ACTGTGTTGT CTAAAGTGAG TCGCCAAGTT GCTTTTGGTG TGCATGAGCT CTTGAAAACC
    AATGCTGCTA ACATCCATTC CAGCAATGAC TGGTACACAC TATTCTGCCT TTTGGAGTGC
    ATTGGGGCAG GAGTGAAGCC GCCCCCTGCC TTACAGGTGA CGACGAGAGC TGACAATGAC
    ACAGGTGCGC AGTCTGACAG TGAAGTTTCC TCATACCATC CAAGTGAAGT GAATCTTGAT
    CGTGGATACA CCTCAGACTC TGAGGTATAT GCAGAACACA GCAAGCAGTC AAAAATGCAC
    CGCTCTGTGA CTGATGTGGA TATGATGAAC AGTGGCTGGC TGATGGTTGG AAAGGAAGAT
    GTTGACTCTT CTAAAGTTGC TACAGTTATT AACAAACCTG TTGCTAATGC CCCAGTGAAT
    CAATACAGCC TAACCATTGG TTTGGATTTT GGCCCTCACG ATACCAAATC TCTCATGAAG
    AGCGTGGAGT CCCTTTCTTT TATTGTTCGA GATGCTGCAC ATGTCACACC AGAAAACTTT
    GAACTCTGTG TTAAAACAAT CCGTGTCTTT GTAGAGGCAA GTCTAAATGG AGGTGTGTGG
    TACAAATCTC AGGAGAAAAG GGTAAAGAAC CACAAATATG ACAGTAAATC CAACCGCTTC
    AAAAAGAAAA CCAAGGAAAA AGAAAATTCT GTCCGCCGTA CACGGGCTTC AAATCAGCGA
    CAATACCGAT CTCACAGTGA TGATGAGGAA GATGAAAGTG TACCAGCCAG CTATCACACT
    GTGTCATTAC AGCTTTTGGA CCTAATGCAC ACACTTCACA CAAGAGCTGC CACCATATAC
    AGTTCTTGGG CAGAGGAACA GCGCAACCTA GAGACAGCTG CTGAGAAAAT TACAGCCGAT
    TCTAAGACAC TGTGGTCAAA CTGTTGGTGT CCTTTACTGC AAGGTATTGC TTGGCTCTGC
    TGTGATGCTC GGCGCCAGGT CAGAATGCAG GCTCTCACCT ACCTGCAAAG GGCACTTTTA
    GTCCATGACT TACAGGCTCT TGATGCACTT GAATGGGAAT CATGCTTTAA TAAGGTGCTA
    TTTCCATTGC TGACCAAACT ATTGGAAAAT ATTAGTCCAG CTGATGTTGG CGGGATGGAG
    GAGACCAGAA TGAGAGGCTC CACTCTGCTC TCAAAGGTAT TTCTACAGCA TCTGTCTCCT
    CTTCTTTCTC TGTCGACATT TGCTGCCTTG TGGCTTACTA TTCTTGATTT CATGGACAAG
    TACATGCATG CTGGCTCCAG TGACTTACTG CCTGAGGCAA TTCCAGAGTC ACTGAAAAAT
    ATGCTTCTCG TAATGGACAC TGCCGGAATA TTCCACAGTG CAGACTCGCG GACGGGCTAC
    TCTGATTTAT GGGAGATCAC GTGGGAAAGG ATAGATTGCT TTTTACCTCG TCTAAGGGAA
    GAACTGTTTA AACAAACTGT GATACAAGAT CCACCCAATA TAGTTACAAC AGAAACTCCT
    GTTGAGCCAA GACCTCCCCC TCAATCAACT CAGTCAGAGA GACCAGCGGA GCAGGGACCT
    TGTGACTCAG AGACTCAAAG TTTAGCAATC TCATCTGCTC CAGTAGTTGG ATCAAGTTCT
    TCCTGCACCC CAGTTAGAGT CAGCCCTGTA ACAGATATAC CTCCACCTAT AACTCAGTCT
    CCAGTCATCC TGCATCCTCT GACCTCCCCG CTGCAGGTGG GAGTGCCACC CATGTCGCTG
    CCAATCATCC TCAACCCTGC ATTAATTGAA GCTACCTCTC CAGTTCCGCT CTTGTCCACT
    CCCCGACCTG CTGATGTATC ATCTGTCTCA GAGGTCAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017951361.1
    CDS162..5723
    Translation

    Target ORF information:

    RefSeq Version XM_018095872.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018095872.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGT TGAAAATGAG AGCTGGAGGA
    ATGAGTGACT CCTCAAAATG GAAAAAGCAG AAGAAATCTC CTAGGCAGCC ACGTCATGTT
    ACCAAAGCTA TGGCGGCAGC AGAACCCTCC TCTCAGCAAC CAGTAGTTAA TGGCACCACA
    ATTACTACTA ACCTCACTGG TGAAGTTCCT TTTATTGATG CTTCCTCTCC AGATGCTAAT
    TTTCCCATTT CAGGGGTGAG TTCAGAGGCC ACGTCTGCTG TAGTCAGTCC TACCACAGAT
    AGTGGTGTAG ATATGTCCTC TCAGGCAACA TCTAAAGAAG ACCTTTCTGA ACTGGATCCT
    GCAAGTCCAT CTCTTTCTGC CATTCAGCAA TCAGGAGACA TTTGTCTTTC TGAACAGCAA
    AACCAGGATG ATGATAAGAT TGAAGACCTT ATGGAAAGGC ATACATATTC TGCTTCTGTG
    GAATCCATAC CAGAAGTATT AGATGATAAT GTGTCATTGG CTGATCAATC AGACTCCGCT
    TCAGTCCATG ATATGGATTA TGTAAATCCT AGAGGTGTGA GATTTACCCA ATCTTCACAG
    AAAGAAGGAA CAGTCTTAGT ACCATATGGA CTGCCATGCA TTCGGGAATT ATTTCGTTTT
    CTTATTTCTT TAACCAACCC CCATGATCGA CATAACTCCG AAGTGATGAT ACACATGGGT
    TTACAGTTGA TCACAGTTGC TCTTGAGTCT GCACCTATTG AAAATTATGT TTCGCTTTTA
    GGCCTTGTAA AGGATGAACT TTGTCGACAC TTATTTCAGC TACTGAGCAT TGAGAGACTG
    AACCTATATG CAGCTTCCTT GCGAGCATGT TTTCTTTTGT TTGAGGGTAT GAGGGAGCAT
    TTGAAGTTCC AGCTAGAGAT GTACATAAAG AAGCTCATGG ATATTATTAC TGTGGAGAAC
    CCTAAGATGC CTTATGAGAT GAAAGAAATG GCTCTTGAAG CTATTGTGCA ATTGTGGAGA
    ATCCCGAGCT TTGTTACAGA ATTATACATT AATTACGACT GTGATTATTA TTGCTCCAAC
    CTTTTTGAAG ATCTCACTAA ACTGCTTTCC AAGAATGCCT TTCCAGTTTC TGGACAGCTG
    TACACCACTC ATCTCCTTTC CCTGGAAGCT CTGCTAACTG TCATTGATAG CACAGAATCA
    CACTGCCAGG CTAAAAATCT AAGCAATGTG TCACAGGATA AGAAAGAAAC TGGAAAACCA
    AGTGGAGATC TGTCAGAAGG GACCAAAGAT TCTGGAAATC TCAATGAACA CTCCATTGAA
    GTTAAGCCAT TCACTGTTAG TGCAGAAAAC AATGGATCGA AGCCCCCCAC AAGTGGCCAC
    TTAATGGCAG ATAAAATGAC TCTTGGGGTC CAAGAAACAG AGTACAGCAC TGATGGAGGT
    GAGAAGAAGA CTTTAAAGAA GCCCCAACGC TTTTCCTGCT TACTTCCTAA TGTTCAAGAG
    CTGAATGACA TTAAGACCAA AAAGAAGCTT CTTATAACTG GAACTGAGCA GTTCAACCAG
    AAACCAAAGA AAGGAGTGCA GTTTCTTCAG GAAAAAGACC TTTTGGCAAC CCCCATGGAT
    AATGCTGAGG TAGCACAGTG GCTCAGAGAA AATCCAAGGC TGGACAAGAA AATGATTGGG
    GAGTTTGTGA GTGATCGCAA AAATCTGGAC TTACTTGAAA GTTTTGTAGG AACCTTCCAT
    TTCCAAGGCC TGAGAGTTGA TGAAGCTTTA AGGCTTTACC TTGAAGCTTT TCGTTTGCCT
    GGCGAGGCAC CTGTGATTCA GAGGTTACTG GAGGCTTTTA CAGAGCACTG GCGAAAATCC
    AATGGAACTC CTTTTGCACA CAGTGATGCC TGCTTTGCTT TGGCTTATGC AGTTATCATG
    CTGAACACAG ACCAACACAA TCACAATGTC CGCAAGCAAA ATGTGCCAAT GACTTTAGAG
    GAATTCAGGA AAAATTTAAA GGGTGTTAAT GGTGGAAAAG ATTTTGACCA AGATATGCTA
    GAGGATATTT ATCACGCAAT CAAAAATGAA GAAATTGTAA TGCCTGAAGA GCAGACTGGC
    TTAGTAAAAG AGAATTATTT TTGGAATGTT TTGCTGCATC GGGGAGCCAC TCCAGAAGGA
    ATGTTCTTGC ATGTGGATCC TGGAAGTTAT GACCATGACC TCTTTACTAT GACTTGGGGG
    CCAACAATAG CTGCCTTGTC CTATGTGTTT GATAAGAGCA TGGATGAAAC GATTATTCAG
    AAAGCAATAT CTGGTTTTAG GAAATGTGCA ATGATATCTG CTCACTATGG TCTCAGTGAT
    GTTTTTGACA ATCTCATAAT TTCCTTGTGC AAGTTTACAA CACTTAGCAG TGAAGCCGTT
    GAAAATCTTC CCACTGTTTT TGGAAGCAAC CTAAAAGCTC AGATTGCTGC AAAGACTGTG
    TTCCACTTAT CCCACAGACA TGGTGACATC CTTCGAGAGG GATGGAAAAA TATTATGGAC
    TCCATGCTGC AGCTTTTTAG AGCTGAGCTG CTTCCCAAGG CTATGGTTGA GGTTGAAGAT
    TTTGTAGACC CCAATGGAAA AATATCTCTT CAACGTGAGG AAATCCCTGC CAACCGTGGG
    GAATCCACTG TACTAAGTTT TGTCAGCTGG CTGACTCTGA GTGGGACAGA ACAGTCAAGT
    CTAAGAGGAC CTTCTACTGA AAATCAAGAG GCCAAGAAAC TAGCGCTGGA ATGTATCAAG
    TTATGTGATC CAGAGAAACT GATCACAGAG AGCAAGTTTC TTCAACTGGA GTCTTTGCAA
    GAACTAATGA AGGCTCTGAT ATCTGTGACA CCAGATGAAG AAACGTATGA TGAAGAAGAT
    GCTGCATTCT GCCTAGAAAT GTTAATGAGA ATTGTGCTAG AAAACAGAGA TCGTGTTGGC
    TGTGTTTGGC AGGCTGTACG AGATCATCTC TATCGTCTAT GTGTCCATGC TGTAGAGTTC
    TGCTTCCTGG TTGAAAGAGC AGTTGTAGGG CTGCTGCGCT TGGCAATACG CCTTTTACGC
    AGAGAGGAAA TTAGTGGTCA GGTTATCCTC TCCTTGAGAA TTCTTTTAAT GATGAAACCA
    ACTGTGTTGT CTAAAGTGAG TCGCCAAGTT GCTTTTGGTG TGCATGAGCT CTTGAAAACC
    AATGCTGCTA ACATCCATTC CAGCAATGAC TGGTACACAC TATTCTGCCT TTTGGAGTGC
    ATTGGGGCAG GAGTGAAGCC GCCCCCTGCC TTACAGGTGA CGACGAGAGC TGACAATGAC
    ACAGGTGCGC AGTCTGACAG TGAAGTTTCC TCATACCATC CAAGTGAAGT GAATCTTGAT
    CGTGGATACA CCTCAGACTC TGAGGTATAT GCAGAACACA GCAAGCAGTC AAAAATGCAC
    CGCTCTGTGA CTGATGTGGA TATGATGAAC AGTGGCTGGC TGATGGTTGG AAAGGAAGAT
    GTTGACTCTT CTAAAGTTGC TACAGTTATT AACAAACCTG TTGCTAATGC CCCAGTGAAT
    CAATACAGCC TAACCATTGG TTTGGATTTT GGCCCTCACG ATACCAAATC TCTCATGAAG
    AGCGTGGAGT CCCTTTCTTT TATTGTTCGA GATGCTGCAC ATGTCACACC AGAAAACTTT
    GAACTCTGTG TTAAAACAAT CCGTGTCTTT GTAGAGGCAA GTCTAAATGG AGGTGTGTGG
    TACAAATCTC AGGAGAAAAG GGTAAAGAAC CACAAATATG ACAGTAAATC CAACCGCTTC
    AAAAAGAAAA CCAAGGAAAA AGAAAATTCT GTCCGCCGTA CACGGGCTTC AAATCAGCGA
    CAATACCGAT CTCACAGTGA TGATGAGGAA GATGAAAGTG TACCAGCCAG CTATCACACT
    GTGTCATTAC AGCTTTTGGA CCTAATGCAC ACACTTCACA CAAGAGCTGC CACCATATAC
    AGTTCTTGGG CAGAGGAACA GCGCAACCTA GAGACAGCTG CTGAGAAAAT TACAGCCGAT
    TCTAAGACAC TGTGGTCAAA CTGTTGGTGT CCTTTACTGC AAGGTATTGC TTGGCTCTGC
    TGTGATGCTC GGCGCCAGGT CAGAATGCAG GCTCTCACCT ACCTGCAAAG GGCACTTTTA
    GTCCATGACT TACAGGCTCT TGATGCACTT GAATGGGAAT CATGCTTTAA TAAGGTGCTA
    TTTCCATTGC TGACCAAACT ATTGGAAAAT ATTAGTCCAG CTGATGTTGG CGGGATGGAG
    GAGACCAGAA TGAGAGGCTC CACTCTGCTC TCAAAGGTAT TTCTACAGCA TCTGTCTCCT
    CTTCTTTCTC TGTCGACATT TGCTGCCTTG TGGCTTACTA TTCTTGATTT CATGGACAAG
    TACATGCATG CTGGCTCCAG TGACTTACTG CCTGAGGCAA TTCCAGAGTC ACTGAAAAAT
    ATGCTTCTCG TAATGGACAC TGCCGGAATA TTCCACAGTG CAGACTCGCG GACGGGCTAC
    TCTGATTTAT GGGAGATCAC GTGGGAAAGG ATAGATTGCT TTTTACCTCG TCTAAGGGAA
    GAACTGTTTA AACAAACTGT GATACAAGAT CCACCCAATA TAGTTACAAC AGAAACTCCT
    GTTGAGCCAA GACCTCCCCC TCAATCAACT CAGTCAGAGA GACCAGCGGA GCAGGGACCT
    TGTGACTCAG AGACTCAAAG TTTAGCAATC TCATCTGCTC CAGTAGTTGG ATCAAGTTCT
    TCCTGCACCC CAGTTAGAGT CAGCCCTGTA ACAGATATAC CTCCACCTAT AACTCAGTCT
    CCAGTCATCC TGCATCCTCT GACCTCCCCG CTGCAGGTGG GAGTGCCACC CATGTCGCTG
    CCAATCATCC TCAACCCTGC ATTAATTGAA GCTACCTCTC CAGTTCCGCT CTTGTCCACT
    CCCCGACCTG CTGATGTATC ATCTGTCTCA GAGGTCAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa12835
    Clone ID Related Accession (Same CDS sequence) XM_018095873.1
    Accession Version XM_018095873.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5556bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGT TGAAAATGAG AGCTGGAGGA
    ATGAGTGACT CCTCAAAATG GAAAAAGCAG AAGAAATCTC CTAGGCAGCC ACGTCATGTT
    ACCAAAGCTA TGGCGGCAGC AGAACCCTCC TCTCAGCAAC CAGTAGTTAA TGGCACCACA
    ATTACTACTA ACCTCACTGG TGAAGTTCCT TTTATTGATG CTTCCTCTCC AGATGCTAAT
    TTTCCCATTT CAGGGGTGAG TTCAGAGGCC ACGTCTGCTG TAGTCAGTCC TACCACAGAT
    AGTGGTGTAG ATATGTCCTC TCAGGCAACA TCTAAAGAAG ACCTTTCTGA ACTGGATCCT
    GCAAGTCCAT CTCTTTCTGC CATTCAGCAA TCAGGAGACA TTTGTCTTTC TGAACAGCAA
    AACCAGGATG ATGATAAGAT TGAAGACCTT ATGGAAAGGC ATACATATTC TGCTTCTGTG
    GAATCCATAC CAGAAGTATT AGATGATAAT GTGTCATTGG CTGATCAATC AGACTCCGCT
    TCAGTCCATG ATATGGATTA TGTAAATCCT AGAGGTGTGA GATTTACCCA ATCTTCACAG
    AAAGAAGGAA CAGTCTTAGT ACCATATGGA CTGCCATGCA TTCGGGAATT ATTTCGTTTT
    CTTATTTCTT TAACCAACCC CCATGATCGA CATAACTCCG AAGTGATGAT ACACATGGGT
    TTACAGTTGA TCACAGTTGC TCTTGAGTCT GCACCTATTG AAAATTATGT TTCGCTTTTA
    GGCCTTGTAA AGGATGAACT TTGTCGACAC TTATTTCAGC TACTGAGCAT TGAGAGACTG
    AACCTATATG CAGCTTCCTT GCGAGCATGT TTTCTTTTGT TTGAGGGTAT GAGGGAGCAT
    TTGAAGTTCC AGCTAGAGAT GTACATAAAG AAGCTCATGG ATATTATTAC TGTGGAGAAC
    CCTAAGATGC CTTATGAGAT GAAAGAAATG GCTCTTGAAG CTATTGTGCA ATTGTGGAGA
    ATCCCGAGCT TTGTTACAGA ATTATACATT AATTACGACT GTGATTATTA TTGCTCCAAC
    CTTTTTGAAG ATCTCACTAA ACTGCTTTCC AAGAATGCCT TTCCAGTTTC TGGACAGCTG
    TACACCACTC ATCTCCTTTC CCTGGAAGCT CTGCTAACTG TCATTGATAG CACAGAATCA
    CACTGCCAGG CTAAAAATCT AAGCAATGTG TCACAGGATA AGAAAGAAAC TGGAAAACCA
    AGTGGAGATC TGTCAGAAGG GACCAAAGAT TCTGGAAATC TCAATGAACA CTCCATTGAA
    GTTAAGCCAT TCACTGTTAG TGCAGAAAAC AATGGATCGA AGCCCCCCAC AAGTGGCCAC
    TTAATGGCAG ATAAAATGAC TCTTGGGGTC CAAGAAACAG AGTACAGCAC TGATGGAGGT
    GAGAAGAAGA CTTTAAAGAA GCCCCAACGC TTTTCCTGCT TACTTCCTAA TGTTCAAGAG
    CTGAATGACA TTAAGACCAA AAAGAAGCTT CTTATAACTG GAACTGAGCA GTTCAACCAG
    AAACCAAAGA AAGGAGTGCA GTTTCTTCAG GAAAAAGACC TTTTGGCAAC CCCCATGGAT
    AATGCTGAGG TAGCACAGTG GCTCAGAGAA AATCCAAGGC TGGACAAGAA AATGATTGGG
    GAGTTTGTGA GTGATCGCAA AAATCTGGAC TTACTTGAAA GTTTTGTAGG AACCTTCCAT
    TTCCAAGGCC TGAGAGTTGA TGAAGCTTTA AGGCTTTACC TTGAAGCTTT TCGTTTGCCT
    GGCGAGGCAC CTGTGATTCA GAGGTTACTG GAGGCTTTTA CAGAGCACTG GCGAAAATCC
    AATGGAACTC CTTTTGCACA CAGTGATGCC TGCTTTGCTT TGGCTTATGC AGTTATCATG
    CTGAACACAG ACCAACACAA TCACAATGTC CGCAAGCAAA ATGTGCCAAT GACTTTAGAG
    GAATTCAGGA AAAATTTAAA GGGTGTTAAT GGTGGAAAAG ATTTTGACCA AGATATGCTA
    GAGGATATTT ATCACGCAAT CAAAAATGAA GAAATTGTAA TGCCTGAAGA GCAGACTGGC
    TTAGTAAAAG AGAATTATTT TTGGAATGTT TTGCTGCATC GGGGAGCCAC TCCAGAAGGA
    ATGTTCTTGC ATGTGGATCC TGGAAGTTAT GACCATGACC TCTTTACTAT GACTTGGGGG
    CCAACAATAG CTGCCTTGTC CTATGTGTTT GATAAGAGCA TGGATGAAAC GATTATTCAG
    AAAGCAATAT CTGGTTTTAG GAAATGTGCA ATGATATCTG CTCACTATGG TCTCAGTGAT
    GTTTTTGACA ATCTCATAAT TTCCTTGTGC AAGTTTACAA CACTTAGCAG TGAAGCCGTT
    GAAAATCTTC CCACTGTTTT TGGAAGCAAC CTAAAAGCTC AGATTGCTGC AAAGACTGTG
    TTCCACTTAT CCCACAGACA TGGTGACATC CTTCGAGAGG GATGGAAAAA TATTATGGAC
    TCCATGCTGC AGCTTTTTAG AGCTGAGCTG CTTCCCAAGG CTATGGTTGA GGTTGAAGAT
    TTTGTAGACC CCAATGGAAA AATATCTCTT CAACGTGAGG AAATCCCTGC CAACCGTGGG
    GAATCCACTG TACTAAGTTT TGTCAGCTGG CTGACTCTGA GTGGGACAGA ACAGTCAAGT
    CTAAGAGGAC CTTCTACTGA AAATCAAGAG GCCAAGAAAC TAGCGCTGGA ATGTATCAAG
    TTATGTGATC CAGAGAAACT GATCACAGAG AGCAAGTTTC TTCAACTGGA GTCTTTGCAA
    GAACTAATGA AGGCTCTGAT ATCTGTGACA CCAGATGAAG AAACGTATGA TGAAGAAGAT
    GCTGCATTCT GCCTAGAAAT GTTAATGAGA ATTGTGCTAG AAAACAGAGA TCGTGTTGGC
    TGTGTTTGGC AGGCTGTACG AGATCATCTC TATCGTCTAT GTGTCCATGC TGTAGAGTTC
    TGCTTCCTGG TTGAAAGAGC AGTTGTAGGG CTGCTGCGCT TGGCAATACG CCTTTTACGC
    AGAGAGGAAA TTAGTGGTCA GGTTATCCTC TCCTTGAGAA TTCTTTTAAT GATGAAACCA
    ACTGTGTTGT CTAAAGTGAG TCGCCAAGTT GCTTTTGGTG TGCATGAGCT CTTGAAAACC
    AATGCTGCTA ACATCCATTC CAGCAATGAC TGGTACACAC TATTCTGCCT TTTGGAGTGC
    ATTGGGGCAG GAGTGAAGCC GCCCCCTGCC TTACAGGTGA CGACGAGAGC TGACAATGAC
    ACAGGTGCGC AGTCTGACAG TGAAGTTTCC TCATACCATC CAAGTGAAGT GAATCTTGAT
    CGTGGATACA CCTCAGACTC TGAGGTATAT GCAGAACACA GCAAGCAGTC AAAAATGCAC
    CGCTCTGTGA CTGATGTGGA TATGATGAAC AGTGGCTGGC TGATGGTTGG AAAGGAAGAT
    GTTGACTCTT CTAAAGTTGC TACAGTTATT AACAAACCTG TTGCTAATGC CCCAGTGAAT
    CAATACAGCC TAACCATTGG TTTGGATTTT GGCCCTCACG ATACCAAATC TCTCATGAAG
    AGCGTGGAGT CCCTTTCTTT TATTGTTCGA GATGCTGCAC ATGTCACACC AGAAAACTTT
    GAACTCTGTG TTAAAACAAT CCGTGTCTTT GTAGAGGCAA GTCTAAATGG AGGGTACAAA
    TCTCAGGAGA AAAGGGTAAA GAACCACAAA TATGACAGTA AATCCAACCG CTTCAAAAAG
    AAAACCAAGG AAAAAGAAAA TTCTGTCCGC CGTACACGGG CTTCAAATCA GCGACAATAC
    CGATCTCACA GTGATGATGA GGAAGATGAA AGTGTACCAG CCAGCTATCA CACTGTGTCA
    TTACAGCTTT TGGACCTAAT GCACACACTT CACACAAGAG CTGCCACCAT ATACAGTTCT
    TGGGCAGAGG AACAGCGCAA CCTAGAGACA GCTGCTGAGA AAATTACAGC CGATTCTAAG
    ACACTGTGGT CAAACTGTTG GTGTCCTTTA CTGCAAGGTA TTGCTTGGCT CTGCTGTGAT
    GCTCGGCGCC AGGTCAGAAT GCAGGCTCTC ACCTACCTGC AAAGGGCACT TTTAGTCCAT
    GACTTACAGG CTCTTGATGC ACTTGAATGG GAATCATGCT TTAATAAGGT GCTATTTCCA
    TTGCTGACCA AACTATTGGA AAATATTAGT CCAGCTGATG TTGGCGGGAT GGAGGAGACC
    AGAATGAGAG GCTCCACTCT GCTCTCAAAG GTATTTCTAC AGCATCTGTC TCCTCTTCTT
    TCTCTGTCGA CATTTGCTGC CTTGTGGCTT ACTATTCTTG ATTTCATGGA CAAGTACATG
    CATGCTGGCT CCAGTGACTT ACTGCCTGAG GCAATTCCAG AGTCACTGAA AAATATGCTT
    CTCGTAATGG ACACTGCCGG AATATTCCAC AGTGCAGACT CGCGGACGGG CTACTCTGAT
    TTATGGGAGA TCACGTGGGA AAGGATAGAT TGCTTTTTAC CTCGTCTAAG GGAAGAACTG
    TTTAAACAAA CTGTGATACA AGATCCACCC AATATAGTTA CAACAGAAAC TCCTGTTGAG
    CCAAGACCTC CCCCTCAATC AACTCAGTCA GAGAGACCAG CGGAGCAGGG ACCTTGTGAC
    TCAGAGACTC AAAGTTTAGC AATCTCATCT GCTCCAGTAG TTGGATCAAG TTCTTCCTGC
    ACCCCAGTTA GAGTCAGCCC TGTAACAGAT ATACCTCCAC CTATAACTCA GTCTCCAGTC
    ATCCTGCATC CTCTGACCTC CCCGCTGCAG GTGGGAGTGC CACCCATGTC GCTGCCAATC
    ATCCTCAACC CTGCATTAAT TGAAGCTACC TCTCCAGTTC CGCTCTTGTC CACTCCCCGA
    CCTGCTGATG TATCATCTGT CTCAGAGGTC AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017951362.1
    CDS162..5717
    Translation

    Target ORF information:

    RefSeq Version XM_018095873.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018095873.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGT TGAAAATGAG AGCTGGAGGA
    ATGAGTGACT CCTCAAAATG GAAAAAGCAG AAGAAATCTC CTAGGCAGCC ACGTCATGTT
    ACCAAAGCTA TGGCGGCAGC AGAACCCTCC TCTCAGCAAC CAGTAGTTAA TGGCACCACA
    ATTACTACTA ACCTCACTGG TGAAGTTCCT TTTATTGATG CTTCCTCTCC AGATGCTAAT
    TTTCCCATTT CAGGGGTGAG TTCAGAGGCC ACGTCTGCTG TAGTCAGTCC TACCACAGAT
    AGTGGTGTAG ATATGTCCTC TCAGGCAACA TCTAAAGAAG ACCTTTCTGA ACTGGATCCT
    GCAAGTCCAT CTCTTTCTGC CATTCAGCAA TCAGGAGACA TTTGTCTTTC TGAACAGCAA
    AACCAGGATG ATGATAAGAT TGAAGACCTT ATGGAAAGGC ATACATATTC TGCTTCTGTG
    GAATCCATAC CAGAAGTATT AGATGATAAT GTGTCATTGG CTGATCAATC AGACTCCGCT
    TCAGTCCATG ATATGGATTA TGTAAATCCT AGAGGTGTGA GATTTACCCA ATCTTCACAG
    AAAGAAGGAA CAGTCTTAGT ACCATATGGA CTGCCATGCA TTCGGGAATT ATTTCGTTTT
    CTTATTTCTT TAACCAACCC CCATGATCGA CATAACTCCG AAGTGATGAT ACACATGGGT
    TTACAGTTGA TCACAGTTGC TCTTGAGTCT GCACCTATTG AAAATTATGT TTCGCTTTTA
    GGCCTTGTAA AGGATGAACT TTGTCGACAC TTATTTCAGC TACTGAGCAT TGAGAGACTG
    AACCTATATG CAGCTTCCTT GCGAGCATGT TTTCTTTTGT TTGAGGGTAT GAGGGAGCAT
    TTGAAGTTCC AGCTAGAGAT GTACATAAAG AAGCTCATGG ATATTATTAC TGTGGAGAAC
    CCTAAGATGC CTTATGAGAT GAAAGAAATG GCTCTTGAAG CTATTGTGCA ATTGTGGAGA
    ATCCCGAGCT TTGTTACAGA ATTATACATT AATTACGACT GTGATTATTA TTGCTCCAAC
    CTTTTTGAAG ATCTCACTAA ACTGCTTTCC AAGAATGCCT TTCCAGTTTC TGGACAGCTG
    TACACCACTC ATCTCCTTTC CCTGGAAGCT CTGCTAACTG TCATTGATAG CACAGAATCA
    CACTGCCAGG CTAAAAATCT AAGCAATGTG TCACAGGATA AGAAAGAAAC TGGAAAACCA
    AGTGGAGATC TGTCAGAAGG GACCAAAGAT TCTGGAAATC TCAATGAACA CTCCATTGAA
    GTTAAGCCAT TCACTGTTAG TGCAGAAAAC AATGGATCGA AGCCCCCCAC AAGTGGCCAC
    TTAATGGCAG ATAAAATGAC TCTTGGGGTC CAAGAAACAG AGTACAGCAC TGATGGAGGT
    GAGAAGAAGA CTTTAAAGAA GCCCCAACGC TTTTCCTGCT TACTTCCTAA TGTTCAAGAG
    CTGAATGACA TTAAGACCAA AAAGAAGCTT CTTATAACTG GAACTGAGCA GTTCAACCAG
    AAACCAAAGA AAGGAGTGCA GTTTCTTCAG GAAAAAGACC TTTTGGCAAC CCCCATGGAT
    AATGCTGAGG TAGCACAGTG GCTCAGAGAA AATCCAAGGC TGGACAAGAA AATGATTGGG
    GAGTTTGTGA GTGATCGCAA AAATCTGGAC TTACTTGAAA GTTTTGTAGG AACCTTCCAT
    TTCCAAGGCC TGAGAGTTGA TGAAGCTTTA AGGCTTTACC TTGAAGCTTT TCGTTTGCCT
    GGCGAGGCAC CTGTGATTCA GAGGTTACTG GAGGCTTTTA CAGAGCACTG GCGAAAATCC
    AATGGAACTC CTTTTGCACA CAGTGATGCC TGCTTTGCTT TGGCTTATGC AGTTATCATG
    CTGAACACAG ACCAACACAA TCACAATGTC CGCAAGCAAA ATGTGCCAAT GACTTTAGAG
    GAATTCAGGA AAAATTTAAA GGGTGTTAAT GGTGGAAAAG ATTTTGACCA AGATATGCTA
    GAGGATATTT ATCACGCAAT CAAAAATGAA GAAATTGTAA TGCCTGAAGA GCAGACTGGC
    TTAGTAAAAG AGAATTATTT TTGGAATGTT TTGCTGCATC GGGGAGCCAC TCCAGAAGGA
    ATGTTCTTGC ATGTGGATCC TGGAAGTTAT GACCATGACC TCTTTACTAT GACTTGGGGG
    CCAACAATAG CTGCCTTGTC CTATGTGTTT GATAAGAGCA TGGATGAAAC GATTATTCAG
    AAAGCAATAT CTGGTTTTAG GAAATGTGCA ATGATATCTG CTCACTATGG TCTCAGTGAT
    GTTTTTGACA ATCTCATAAT TTCCTTGTGC AAGTTTACAA CACTTAGCAG TGAAGCCGTT
    GAAAATCTTC CCACTGTTTT TGGAAGCAAC CTAAAAGCTC AGATTGCTGC AAAGACTGTG
    TTCCACTTAT CCCACAGACA TGGTGACATC CTTCGAGAGG GATGGAAAAA TATTATGGAC
    TCCATGCTGC AGCTTTTTAG AGCTGAGCTG CTTCCCAAGG CTATGGTTGA GGTTGAAGAT
    TTTGTAGACC CCAATGGAAA AATATCTCTT CAACGTGAGG AAATCCCTGC CAACCGTGGG
    GAATCCACTG TACTAAGTTT TGTCAGCTGG CTGACTCTGA GTGGGACAGA ACAGTCAAGT
    CTAAGAGGAC CTTCTACTGA AAATCAAGAG GCCAAGAAAC TAGCGCTGGA ATGTATCAAG
    TTATGTGATC CAGAGAAACT GATCACAGAG AGCAAGTTTC TTCAACTGGA GTCTTTGCAA
    GAACTAATGA AGGCTCTGAT ATCTGTGACA CCAGATGAAG AAACGTATGA TGAAGAAGAT
    GCTGCATTCT GCCTAGAAAT GTTAATGAGA ATTGTGCTAG AAAACAGAGA TCGTGTTGGC
    TGTGTTTGGC AGGCTGTACG AGATCATCTC TATCGTCTAT GTGTCCATGC TGTAGAGTTC
    TGCTTCCTGG TTGAAAGAGC AGTTGTAGGG CTGCTGCGCT TGGCAATACG CCTTTTACGC
    AGAGAGGAAA TTAGTGGTCA GGTTATCCTC TCCTTGAGAA TTCTTTTAAT GATGAAACCA
    ACTGTGTTGT CTAAAGTGAG TCGCCAAGTT GCTTTTGGTG TGCATGAGCT CTTGAAAACC
    AATGCTGCTA ACATCCATTC CAGCAATGAC TGGTACACAC TATTCTGCCT TTTGGAGTGC
    ATTGGGGCAG GAGTGAAGCC GCCCCCTGCC TTACAGGTGA CGACGAGAGC TGACAATGAC
    ACAGGTGCGC AGTCTGACAG TGAAGTTTCC TCATACCATC CAAGTGAAGT GAATCTTGAT
    CGTGGATACA CCTCAGACTC TGAGGTATAT GCAGAACACA GCAAGCAGTC AAAAATGCAC
    CGCTCTGTGA CTGATGTGGA TATGATGAAC AGTGGCTGGC TGATGGTTGG AAAGGAAGAT
    GTTGACTCTT CTAAAGTTGC TACAGTTATT AACAAACCTG TTGCTAATGC CCCAGTGAAT
    CAATACAGCC TAACCATTGG TTTGGATTTT GGCCCTCACG ATACCAAATC TCTCATGAAG
    AGCGTGGAGT CCCTTTCTTT TATTGTTCGA GATGCTGCAC ATGTCACACC AGAAAACTTT
    GAACTCTGTG TTAAAACAAT CCGTGTCTTT GTAGAGGCAA GTCTAAATGG AGGGTACAAA
    TCTCAGGAGA AAAGGGTAAA GAACCACAAA TATGACAGTA AATCCAACCG CTTCAAAAAG
    AAAACCAAGG AAAAAGAAAA TTCTGTCCGC CGTACACGGG CTTCAAATCA GCGACAATAC
    CGATCTCACA GTGATGATGA GGAAGATGAA AGTGTACCAG CCAGCTATCA CACTGTGTCA
    TTACAGCTTT TGGACCTAAT GCACACACTT CACACAAGAG CTGCCACCAT ATACAGTTCT
    TGGGCAGAGG AACAGCGCAA CCTAGAGACA GCTGCTGAGA AAATTACAGC CGATTCTAAG
    ACACTGTGGT CAAACTGTTG GTGTCCTTTA CTGCAAGGTA TTGCTTGGCT CTGCTGTGAT
    GCTCGGCGCC AGGTCAGAAT GCAGGCTCTC ACCTACCTGC AAAGGGCACT TTTAGTCCAT
    GACTTACAGG CTCTTGATGC ACTTGAATGG GAATCATGCT TTAATAAGGT GCTATTTCCA
    TTGCTGACCA AACTATTGGA AAATATTAGT CCAGCTGATG TTGGCGGGAT GGAGGAGACC
    AGAATGAGAG GCTCCACTCT GCTCTCAAAG GTATTTCTAC AGCATCTGTC TCCTCTTCTT
    TCTCTGTCGA CATTTGCTGC CTTGTGGCTT ACTATTCTTG ATTTCATGGA CAAGTACATG
    CATGCTGGCT CCAGTGACTT ACTGCCTGAG GCAATTCCAG AGTCACTGAA AAATATGCTT
    CTCGTAATGG ACACTGCCGG AATATTCCAC AGTGCAGACT CGCGGACGGG CTACTCTGAT
    TTATGGGAGA TCACGTGGGA AAGGATAGAT TGCTTTTTAC CTCGTCTAAG GGAAGAACTG
    TTTAAACAAA CTGTGATACA AGATCCACCC AATATAGTTA CAACAGAAAC TCCTGTTGAG
    CCAAGACCTC CCCCTCAATC AACTCAGTCA GAGAGACCAG CGGAGCAGGG ACCTTGTGAC
    TCAGAGACTC AAAGTTTAGC AATCTCATCT GCTCCAGTAG TTGGATCAAG TTCTTCCTGC
    ACCCCAGTTA GAGTCAGCCC TGTAACAGAT ATACCTCCAC CTATAACTCA GTCTCCAGTC
    ATCCTGCATC CTCTGACCTC CCCGCTGCAG GTGGGAGTGC CACCCATGTC GCTGCCAATC
    ATCCTCAACC CTGCATTAAT TGAAGCTACC TCTCCAGTTC CGCTCTTGTC CACTCCCCGA
    CCTGCTGATG TATCATCTGT CTCAGAGGTC AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa33097
    Clone ID Related Accession (Same CDS sequence) XM_012966852.2 , XM_012966854.2 , XM_012966853.2 , XM_012966851.2
    Accession Version XM_012966852.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5574bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966852.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGT TGAAAATGAG AGCTGGAGGA
    ATGAGTGACT CCTCAAAATG GAAAAAGCAG AAGAAATCTC CTAGGCAGCC ACGTCATGTT
    ACCAAAGCTA TGGCGGCAGC AGAACCCTCC TCTCAGCAAC CAGTAGTTAA TGGCACCACA
    ATTACTACTA ACCTCACTGG TGAAGTTCCT TTTATTGATG CTTCCTCTCC AGATGCTAAT
    TTTCCCATTT CAGGGGTGAG TTCAGAGGCC ACGTCTGCTG TAGTCAGTCC TACCACAGAT
    AGTGGTGTAG ATATGTCCTC TCAGGCAACA TCTAAAGAAG ACCTTTCTGA ACTGGATCCT
    GCAAGTCCAT CTCTTTCTGC CATTCAGCAA TCAGGAGACA TTTGTCTTTC TGAACAGCAA
    AACCAGGATG ATGATAAGAT TGAAGACCTT ATGGAAAGGC ATACATATTC TGCTTCTGTG
    GAATCCATAC CAGAAGTATT AGATGATAAT GTGTCATTGG CTGATCAATC AGACTCCGCT
    TCAGTCCATG ATATGGATTA TGTAAATCCT AGAGGTGTGA GATTTACCCA ATCTTCACAG
    AAAGAAGGAA CAGTCTTAGT ACCATATGGA CTGCCATGCA TTCGGGAATT ATTTCGTTTT
    CTTATTTCTT TAACCAACCC CCATGATCGA CATAACTCCG AAGTGATGAT ACACATGGGT
    TTACAGTTGA TCACAGTTGC TCTTGAGTCT GCACCTATTG AAAATTATGT TTCGCTTTTA
    GGCCTTGTAA AGGATGAACT TTGTCGACAC TTATTTCAGC TACTGAGCAT TGAGAGACTG
    AACCTATATG CAGCTTCCTT GCGAGCATGT TTTCTTTTGT TTGAGGGTAT GAGGGAGCAT
    TTGAAGTTCC AGCTAGAGAT GTACATAAAG AAGCTCATGG ATATTATTAC TGTGGAGAAC
    CCTAAGATGC CTTATGAGAT GAAAGAAATG GCTCTTGAAG CTATTGTGCA ATTGTGGAGA
    ATCCCGAGCT TTGTTACAGA ATTATACATT AATTACGACT GTGATTATTA TTGCTCCAAC
    CTTTTTGAAG ATCTCACTAA ACTGCTTTCC AAGAATGCCT TTCCAGTTTC TGGACAGCTG
    TACACCACTC ATCTCCTTTC CCTGGAAGCT CTGCTAACTG TCATTGATAG CACAGAATCA
    CACTGCCAGG CTAAAAATCT AAGCAATGTG TCACAGGATA AGAAAGAAAC TGGAAAACCA
    AGTGGAGATC TGTCAGAAGG GACCAAAGAT TCTGGAAATC TCAATGAACA CTCCATTGAA
    GTTAAGCCAT TCACTGTTAG TGCAGAAAAC AATGGATCGA AGCCCCCCAC AAGTGGCCAC
    TTAATGGCAG ATAAAATGAC TCTTGGGGTC CAAGAAACAG AGTACAGCAC TGATGGAGGT
    GAGAAGAAGA CTTTAAAGAA GCCCCAACGC TTTTCCTGCT TACTTCCTAA TGTTCAAGAG
    CTGAATGACA TTAAGACCAA AAAGAAGCTT CTTATAACTG GAACTGAGCA GTTCAACCAG
    AAACCAAAGA AAGGAGTGCA GTTTCTTCAG GAAAAAGACC TTTTGGCAAC CCCCATGGAT
    AATGCTGAGG TAGCACAGTG GCTCAGAGAA AATCCAAGGC TGGACAAGAA AATGATTGGG
    GAGTTTGTGA GTGATCGCAA AAATCTGGAC TTACTTGAAA GTTTTGTAGG AACCTTCCAT
    TTCCAAGGCC TGAGAGTTGA TGAAGCTTTA AGGCTTTACC TTGAAGCTTT TCGTTTGCCT
    GGCGAGGCAC CTGTGATTCA GAGGTTACTG GAGGCTTTTA CAGAGCACTG GCGAAAATCC
    AATGGAACTC CTTTTGCACA CAGTGATGCC TGCTTTGCTT TGGCTTATGC AGTTATCATG
    CTGAACACAG ACCAACACAA TCACAATGTC CGCAAGCAAA ATGTGCCAAT GACTTTAGAG
    GAATTCAGGA AAAATTTAAA GGGTGTTAAT GGTGGAAAAG ATTTTGACCA AGATATGCTA
    GAGGATATTT ATCACGCAAT CAAAAATGAA GAAATTGTAA TGCCTGAAGA GCAGACTGGC
    TTAGTAAAAG AGAATTATTT TTGGAATGTT TTGCTGCATC GGGGAGCCAC TCCAGAAGGA
    ATGTTCTTGC ATGTGGATCC TGGAAGTTAT GACCATGACC TCTTTACTAT GACTTGGGGG
    CCAACAATAG CTGCCTTGTC CTATGTGTTT GATAAGAGCA TGGATGAAAC GATTATTCAG
    AAAGCAATAT CTGGTTTTAG GAAATGTGCA ATGATATCTG CTCACTATGG TCTCAGTGAT
    GTTTTTGACA ATCTCATAAT TTCCTTGTGC AAGTTTACAA CACTTAGCAG TGAAGCCGTT
    GAAAATCTTC CCACTGTTTT TGGAAGCAAC CTAAAAGCTC AGATTGCTGC AAAGACTGTG
    TTCCACTTAT CCCACAGACA TGGTGACATC CTTCGAGAGG GATGGAAAAA TATTATGGAC
    TCCATGCTGC AGCTTTTTAG AGCTGAGCTG CTTCCCAAGG CTATGGTTGA GGTTGAAGAT
    TTTGTAGACC CCAATGGAAA AATATCTCTT CAACGTGAGG AAATCCCTGC CAACCGTGGG
    GAATCCACTG TACTAAGTTT TGTCAGCTGG CTGACTCTGA GTGGGACAGA ACAGTCAAGT
    CTAAGAGGAC CTTCTACTGA AAATCAAGAG GCCAAGAAAC TAGCGCTGGA ATGTATCAAG
    TTATGTGATC CAGAGAAACT GATCACAGAG AGCAAGTTTC TTCAACTGGA GTCTTTGCAA
    GAACTAATGA AGGCTCTGAT ATCTGTGACA CCAGATGAAG AAACGTATGA TGAAGAAGAT
    GCTGCATTCT GCCTAGAAAT GTTAATGAGA ATTGTGCTAG AAAACAGAGA TCGTGTTGGC
    TGTGTTTGGC AGGCTGTACG AGATCATCTC TATCGTCTAT GTGTCCATGC TGTAGAGTTC
    TGCTTCCTGG TTGAAAGAGC AGTTGTAGGG CTGCTGCGCT TGGCAATACG CCTTTTACGC
    AGAGAGGAAA TTAGTGGTCA GGTTATCCTC TCCTTGAGAA TTCTTTTAAT GATGAAACCA
    ACTGTGTTGT CTAAAGTGAG TCGCCAAGTT GCTTTTGGTG TGCATGAGCT CTTGAAAACC
    AATGCTGCTA ACATCCATTC CAGCAATGAC TGGTACACAC TATTCTGCCT TTTGGAGTGC
    ATTGGGGCAG GAGTGAAGCC GCCCCCTGCC TTACAGGTGA CGACGAGAGC TGACAATGAC
    ACAGGTGCGC AGTCTGACAG TGAAGTTTCC TCATACCATC CAAGTGAAGT GAATCTTGAT
    CGTGGATACA CCTCAGACTC TGAGGTATAT GCAGAACACA GCAAGCAGTC AAAAATGCAC
    CGCTCTGTGA CTGATGTGGA TATGATGAAC AGTGGCTGGC TGATGGTTGG AAAGGAAGAT
    GTTGACTCTT CTAAAGTTGC TACAGTTATT AACAAACCTG TTGCTAATGC CCCAGTGAAT
    CAATACAGCC TAACCATTGG TTTGGATTTT GGCCCTCACG ATACCAAATC TCTCATGAAG
    AGCGTGGAGT CCCTTTCTTT TATTGTTCGA GATGCTGCAC ATGTCACACC AGAAAACTTT
    GAACTCTGTG TTAAAACAAT CCGTGTCTTT GTAGAGGCAA GTCTAAATGG AGGTGTGTGG
    TACAAATCTC AGGAGAAAAG GGTAAAGAAC CACAAATATG ACAGTAAATC CAACCGCTTC
    AAAAAGAAAA CCAAGGAAAA AGAAAATTCT GTCCGCCGTA CACGGGCTTC AAATCAGCGA
    CAATACCGAT CTCACAGTGA TGATGAGGAA GATGAAAGTG TACCAGCCAG CTATCACACT
    GTGTCATTAC AGGTTAGTCA TGATCTTTTG GACCTAATGC ACACACTTCA CACAAGAGCT
    GCCACCATAT ACAGTTCTTG GGCAGAGGAA CAGCGCAACC TAGAGACAGC TGCTGAGAAA
    ATTACAGCCG ATTCTAAGAC ACTGTGGTCA AACTGTTGGT GTCCTTTACT GCAAGGTATT
    GCTTGGCTCT GCTGTGATGC TCGGCGCCAG GTCAGAATGC AGGCTCTCAC CTACCTGCAA
    AGGGCACTTT TAGTCCATGA CTTACAGGCT CTTGATGCAC TTGAATGGGA ATCATGCTTT
    AATAAGGTGC TATTTCCATT GCTGACCAAA CTATTGGAAA ATATTAGTCC AGCTGATGTT
    GGCGGGATGG AGGAGACCAG AATGAGAGGC TCCACTCTGC TCTCAAAGGT ATTTCTACAG
    CATCTGTCTC CTCTTCTTTC TCTGTCGACA TTTGCTGCCT TGTGGCTTAC TATTCTTGAT
    TTCATGGACA AGTACATGCA TGCTGGCTCC AGTGACTTAC TGCCTGAGGC AATTCCAGAG
    TCACTGAAAA ATATGCTTCT CGTAATGGAC ACTGCCGGAA TATTCCACAG TGCAGACTCG
    CGGACGGGCT ACTCTGATTT ATGGGAGATC ACGTGGGAAA GGATAGATTG CTTTTTACCT
    CGTCTAAGGG AAGAACTGTT TAAACAAACT GTGATACAAG ATCCACCCAA TATAGTTACA
    ACAGAAACTC CTGTTGAGCC AAGACCTCCC CCTCAATCAA CTCAGTCAGA GAGACCAGCG
    GAGCAGGGAC CTTGTGACTC AGAGACTCAA AGTTTAGCAA TCTCATCTGC TCCAGTAGTT
    GGATCAAGTT CTTCCTGCAC CCCAGTTAGA GTCAGCCCTG TAACAGATAT ACCTCCACCT
    ATAACTCAGT CTCCAGTCAT CCTGCATCCT CTGACCTCCC CGCTGCAGGT GGGAGTGCCA
    CCCATGTCGC TGCCAATCAT CCTCAACCCT GCATTAATTG AAGCTACCTC TCCAGTTCCG
    CTCTTGTCCA CTCCCCGACC TGCTGATGTA TCATCTGTCT CAGAGGTCAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012822306.1
    CDS163..5736
    Translation

    Target ORF information:

    RefSeq Version XM_012966852.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966852.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGT TGAAAATGAG AGCTGGAGGA
    ATGAGTGACT CCTCAAAATG GAAAAAGCAG AAGAAATCTC CTAGGCAGCC ACGTCATGTT
    ACCAAAGCTA TGGCGGCAGC AGAACCCTCC TCTCAGCAAC CAGTAGTTAA TGGCACCACA
    ATTACTACTA ACCTCACTGG TGAAGTTCCT TTTATTGATG CTTCCTCTCC AGATGCTAAT
    TTTCCCATTT CAGGGGTGAG TTCAGAGGCC ACGTCTGCTG TAGTCAGTCC TACCACAGAT
    AGTGGTGTAG ATATGTCCTC TCAGGCAACA TCTAAAGAAG ACCTTTCTGA ACTGGATCCT
    GCAAGTCCAT CTCTTTCTGC CATTCAGCAA TCAGGAGACA TTTGTCTTTC TGAACAGCAA
    AACCAGGATG ATGATAAGAT TGAAGACCTT ATGGAAAGGC ATACATATTC TGCTTCTGTG
    GAATCCATAC CAGAAGTATT AGATGATAAT GTGTCATTGG CTGATCAATC AGACTCCGCT
    TCAGTCCATG ATATGGATTA TGTAAATCCT AGAGGTGTGA GATTTACCCA ATCTTCACAG
    AAAGAAGGAA CAGTCTTAGT ACCATATGGA CTGCCATGCA TTCGGGAATT ATTTCGTTTT
    CTTATTTCTT TAACCAACCC CCATGATCGA CATAACTCCG AAGTGATGAT ACACATGGGT
    TTACAGTTGA TCACAGTTGC TCTTGAGTCT GCACCTATTG AAAATTATGT TTCGCTTTTA
    GGCCTTGTAA AGGATGAACT TTGTCGACAC TTATTTCAGC TACTGAGCAT TGAGAGACTG
    AACCTATATG CAGCTTCCTT GCGAGCATGT TTTCTTTTGT TTGAGGGTAT GAGGGAGCAT
    TTGAAGTTCC AGCTAGAGAT GTACATAAAG AAGCTCATGG ATATTATTAC TGTGGAGAAC
    CCTAAGATGC CTTATGAGAT GAAAGAAATG GCTCTTGAAG CTATTGTGCA ATTGTGGAGA
    ATCCCGAGCT TTGTTACAGA ATTATACATT AATTACGACT GTGATTATTA TTGCTCCAAC
    CTTTTTGAAG ATCTCACTAA ACTGCTTTCC AAGAATGCCT TTCCAGTTTC TGGACAGCTG
    TACACCACTC ATCTCCTTTC CCTGGAAGCT CTGCTAACTG TCATTGATAG CACAGAATCA
    CACTGCCAGG CTAAAAATCT AAGCAATGTG TCACAGGATA AGAAAGAAAC TGGAAAACCA
    AGTGGAGATC TGTCAGAAGG GACCAAAGAT TCTGGAAATC TCAATGAACA CTCCATTGAA
    GTTAAGCCAT TCACTGTTAG TGCAGAAAAC AATGGATCGA AGCCCCCCAC AAGTGGCCAC
    TTAATGGCAG ATAAAATGAC TCTTGGGGTC CAAGAAACAG AGTACAGCAC TGATGGAGGT
    GAGAAGAAGA CTTTAAAGAA GCCCCAACGC TTTTCCTGCT TACTTCCTAA TGTTCAAGAG
    CTGAATGACA TTAAGACCAA AAAGAAGCTT CTTATAACTG GAACTGAGCA GTTCAACCAG
    AAACCAAAGA AAGGAGTGCA GTTTCTTCAG GAAAAAGACC TTTTGGCAAC CCCCATGGAT
    AATGCTGAGG TAGCACAGTG GCTCAGAGAA AATCCAAGGC TGGACAAGAA AATGATTGGG
    GAGTTTGTGA GTGATCGCAA AAATCTGGAC TTACTTGAAA GTTTTGTAGG AACCTTCCAT
    TTCCAAGGCC TGAGAGTTGA TGAAGCTTTA AGGCTTTACC TTGAAGCTTT TCGTTTGCCT
    GGCGAGGCAC CTGTGATTCA GAGGTTACTG GAGGCTTTTA CAGAGCACTG GCGAAAATCC
    AATGGAACTC CTTTTGCACA CAGTGATGCC TGCTTTGCTT TGGCTTATGC AGTTATCATG
    CTGAACACAG ACCAACACAA TCACAATGTC CGCAAGCAAA ATGTGCCAAT GACTTTAGAG
    GAATTCAGGA AAAATTTAAA GGGTGTTAAT GGTGGAAAAG ATTTTGACCA AGATATGCTA
    GAGGATATTT ATCACGCAAT CAAAAATGAA GAAATTGTAA TGCCTGAAGA GCAGACTGGC
    TTAGTAAAAG AGAATTATTT TTGGAATGTT TTGCTGCATC GGGGAGCCAC TCCAGAAGGA
    ATGTTCTTGC ATGTGGATCC TGGAAGTTAT GACCATGACC TCTTTACTAT GACTTGGGGG
    CCAACAATAG CTGCCTTGTC CTATGTGTTT GATAAGAGCA TGGATGAAAC GATTATTCAG
    AAAGCAATAT CTGGTTTTAG GAAATGTGCA ATGATATCTG CTCACTATGG TCTCAGTGAT
    GTTTTTGACA ATCTCATAAT TTCCTTGTGC AAGTTTACAA CACTTAGCAG TGAAGCCGTT
    GAAAATCTTC CCACTGTTTT TGGAAGCAAC CTAAAAGCTC AGATTGCTGC AAAGACTGTG
    TTCCACTTAT CCCACAGACA TGGTGACATC CTTCGAGAGG GATGGAAAAA TATTATGGAC
    TCCATGCTGC AGCTTTTTAG AGCTGAGCTG CTTCCCAAGG CTATGGTTGA GGTTGAAGAT
    TTTGTAGACC CCAATGGAAA AATATCTCTT CAACGTGAGG AAATCCCTGC CAACCGTGGG
    GAATCCACTG TACTAAGTTT TGTCAGCTGG CTGACTCTGA GTGGGACAGA ACAGTCAAGT
    CTAAGAGGAC CTTCTACTGA AAATCAAGAG GCCAAGAAAC TAGCGCTGGA ATGTATCAAG
    TTATGTGATC CAGAGAAACT GATCACAGAG AGCAAGTTTC TTCAACTGGA GTCTTTGCAA
    GAACTAATGA AGGCTCTGAT ATCTGTGACA CCAGATGAAG AAACGTATGA TGAAGAAGAT
    GCTGCATTCT GCCTAGAAAT GTTAATGAGA ATTGTGCTAG AAAACAGAGA TCGTGTTGGC
    TGTGTTTGGC AGGCTGTACG AGATCATCTC TATCGTCTAT GTGTCCATGC TGTAGAGTTC
    TGCTTCCTGG TTGAAAGAGC AGTTGTAGGG CTGCTGCGCT TGGCAATACG CCTTTTACGC
    AGAGAGGAAA TTAGTGGTCA GGTTATCCTC TCCTTGAGAA TTCTTTTAAT GATGAAACCA
    ACTGTGTTGT CTAAAGTGAG TCGCCAAGTT GCTTTTGGTG TGCATGAGCT CTTGAAAACC
    AATGCTGCTA ACATCCATTC CAGCAATGAC TGGTACACAC TATTCTGCCT TTTGGAGTGC
    ATTGGGGCAG GAGTGAAGCC GCCCCCTGCC TTACAGGTGA CGACGAGAGC TGACAATGAC
    ACAGGTGCGC AGTCTGACAG TGAAGTTTCC TCATACCATC CAAGTGAAGT GAATCTTGAT
    CGTGGATACA CCTCAGACTC TGAGGTATAT GCAGAACACA GCAAGCAGTC AAAAATGCAC
    CGCTCTGTGA CTGATGTGGA TATGATGAAC AGTGGCTGGC TGATGGTTGG AAAGGAAGAT
    GTTGACTCTT CTAAAGTTGC TACAGTTATT AACAAACCTG TTGCTAATGC CCCAGTGAAT
    CAATACAGCC TAACCATTGG TTTGGATTTT GGCCCTCACG ATACCAAATC TCTCATGAAG
    AGCGTGGAGT CCCTTTCTTT TATTGTTCGA GATGCTGCAC ATGTCACACC AGAAAACTTT
    GAACTCTGTG TTAAAACAAT CCGTGTCTTT GTAGAGGCAA GTCTAAATGG AGGTGTGTGG
    TACAAATCTC AGGAGAAAAG GGTAAAGAAC CACAAATATG ACAGTAAATC CAACCGCTTC
    AAAAAGAAAA CCAAGGAAAA AGAAAATTCT GTCCGCCGTA CACGGGCTTC AAATCAGCGA
    CAATACCGAT CTCACAGTGA TGATGAGGAA GATGAAAGTG TACCAGCCAG CTATCACACT
    GTGTCATTAC AGGTTAGTCA TGATCTTTTG GACCTAATGC ACACACTTCA CACAAGAGCT
    GCCACCATAT ACAGTTCTTG GGCAGAGGAA CAGCGCAACC TAGAGACAGC TGCTGAGAAA
    ATTACAGCCG ATTCTAAGAC ACTGTGGTCA AACTGTTGGT GTCCTTTACT GCAAGGTATT
    GCTTGGCTCT GCTGTGATGC TCGGCGCCAG GTCAGAATGC AGGCTCTCAC CTACCTGCAA
    AGGGCACTTT TAGTCCATGA CTTACAGGCT CTTGATGCAC TTGAATGGGA ATCATGCTTT
    AATAAGGTGC TATTTCCATT GCTGACCAAA CTATTGGAAA ATATTAGTCC AGCTGATGTT
    GGCGGGATGG AGGAGACCAG AATGAGAGGC TCCACTCTGC TCTCAAAGGT ATTTCTACAG
    CATCTGTCTC CTCTTCTTTC TCTGTCGACA TTTGCTGCCT TGTGGCTTAC TATTCTTGAT
    TTCATGGACA AGTACATGCA TGCTGGCTCC AGTGACTTAC TGCCTGAGGC AATTCCAGAG
    TCACTGAAAA ATATGCTTCT CGTAATGGAC ACTGCCGGAA TATTCCACAG TGCAGACTCG
    CGGACGGGCT ACTCTGATTT ATGGGAGATC ACGTGGGAAA GGATAGATTG CTTTTTACCT
    CGTCTAAGGG AAGAACTGTT TAAACAAACT GTGATACAAG ATCCACCCAA TATAGTTACA
    ACAGAAACTC CTGTTGAGCC AAGACCTCCC CCTCAATCAA CTCAGTCAGA GAGACCAGCG
    GAGCAGGGAC CTTGTGACTC AGAGACTCAA AGTTTAGCAA TCTCATCTGC TCCAGTAGTT
    GGATCAAGTT CTTCCTGCAC CCCAGTTAGA GTCAGCCCTG TAACAGATAT ACCTCCACCT
    ATAACTCAGT CTCCAGTCAT CCTGCATCCT CTGACCTCCC CGCTGCAGGT GGGAGTGCCA
    CCCATGTCGC TGCCAATCAT CCTCAACCCT GCATTAATTG AAGCTACCTC TCCAGTTCCG
    CTCTTGTCCA CTCCCCGACC TGCTGATGTA TCATCTGTCT CAGAGGTCAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa33097
    Clone ID Related Accession (Same CDS sequence) XM_012966852.2 , XM_012966854.2 , XM_012966853.2 , XM_012966851.2
    Accession Version XM_012966854.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5574bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966854.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGT TGAAAATGAG AGCTGGAGGA
    ATGAGTGACT CCTCAAAATG GAAAAAGCAG AAGAAATCTC CTAGGCAGCC ACGTCATGTT
    ACCAAAGCTA TGGCGGCAGC AGAACCCTCC TCTCAGCAAC CAGTAGTTAA TGGCACCACA
    ATTACTACTA ACCTCACTGG TGAAGTTCCT TTTATTGATG CTTCCTCTCC AGATGCTAAT
    TTTCCCATTT CAGGGGTGAG TTCAGAGGCC ACGTCTGCTG TAGTCAGTCC TACCACAGAT
    AGTGGTGTAG ATATGTCCTC TCAGGCAACA TCTAAAGAAG ACCTTTCTGA ACTGGATCCT
    GCAAGTCCAT CTCTTTCTGC CATTCAGCAA TCAGGAGACA TTTGTCTTTC TGAACAGCAA
    AACCAGGATG ATGATAAGAT TGAAGACCTT ATGGAAAGGC ATACATATTC TGCTTCTGTG
    GAATCCATAC CAGAAGTATT AGATGATAAT GTGTCATTGG CTGATCAATC AGACTCCGCT
    TCAGTCCATG ATATGGATTA TGTAAATCCT AGAGGTGTGA GATTTACCCA ATCTTCACAG
    AAAGAAGGAA CAGTCTTAGT ACCATATGGA CTGCCATGCA TTCGGGAATT ATTTCGTTTT
    CTTATTTCTT TAACCAACCC CCATGATCGA CATAACTCCG AAGTGATGAT ACACATGGGT
    TTACAGTTGA TCACAGTTGC TCTTGAGTCT GCACCTATTG AAAATTATGT TTCGCTTTTA
    GGCCTTGTAA AGGATGAACT TTGTCGACAC TTATTTCAGC TACTGAGCAT TGAGAGACTG
    AACCTATATG CAGCTTCCTT GCGAGCATGT TTTCTTTTGT TTGAGGGTAT GAGGGAGCAT
    TTGAAGTTCC AGCTAGAGAT GTACATAAAG AAGCTCATGG ATATTATTAC TGTGGAGAAC
    CCTAAGATGC CTTATGAGAT GAAAGAAATG GCTCTTGAAG CTATTGTGCA ATTGTGGAGA
    ATCCCGAGCT TTGTTACAGA ATTATACATT AATTACGACT GTGATTATTA TTGCTCCAAC
    CTTTTTGAAG ATCTCACTAA ACTGCTTTCC AAGAATGCCT TTCCAGTTTC TGGACAGCTG
    TACACCACTC ATCTCCTTTC CCTGGAAGCT CTGCTAACTG TCATTGATAG CACAGAATCA
    CACTGCCAGG CTAAAAATCT AAGCAATGTG TCACAGGATA AGAAAGAAAC TGGAAAACCA
    AGTGGAGATC TGTCAGAAGG GACCAAAGAT TCTGGAAATC TCAATGAACA CTCCATTGAA
    GTTAAGCCAT TCACTGTTAG TGCAGAAAAC AATGGATCGA AGCCCCCCAC AAGTGGCCAC
    TTAATGGCAG ATAAAATGAC TCTTGGGGTC CAAGAAACAG AGTACAGCAC TGATGGAGGT
    GAGAAGAAGA CTTTAAAGAA GCCCCAACGC TTTTCCTGCT TACTTCCTAA TGTTCAAGAG
    CTGAATGACA TTAAGACCAA AAAGAAGCTT CTTATAACTG GAACTGAGCA GTTCAACCAG
    AAACCAAAGA AAGGAGTGCA GTTTCTTCAG GAAAAAGACC TTTTGGCAAC CCCCATGGAT
    AATGCTGAGG TAGCACAGTG GCTCAGAGAA AATCCAAGGC TGGACAAGAA AATGATTGGG
    GAGTTTGTGA GTGATCGCAA AAATCTGGAC TTACTTGAAA GTTTTGTAGG AACCTTCCAT
    TTCCAAGGCC TGAGAGTTGA TGAAGCTTTA AGGCTTTACC TTGAAGCTTT TCGTTTGCCT
    GGCGAGGCAC CTGTGATTCA GAGGTTACTG GAGGCTTTTA CAGAGCACTG GCGAAAATCC
    AATGGAACTC CTTTTGCACA CAGTGATGCC TGCTTTGCTT TGGCTTATGC AGTTATCATG
    CTGAACACAG ACCAACACAA TCACAATGTC CGCAAGCAAA ATGTGCCAAT GACTTTAGAG
    GAATTCAGGA AAAATTTAAA GGGTGTTAAT GGTGGAAAAG ATTTTGACCA AGATATGCTA
    GAGGATATTT ATCACGCAAT CAAAAATGAA GAAATTGTAA TGCCTGAAGA GCAGACTGGC
    TTAGTAAAAG AGAATTATTT TTGGAATGTT TTGCTGCATC GGGGAGCCAC TCCAGAAGGA
    ATGTTCTTGC ATGTGGATCC TGGAAGTTAT GACCATGACC TCTTTACTAT GACTTGGGGG
    CCAACAATAG CTGCCTTGTC CTATGTGTTT GATAAGAGCA TGGATGAAAC GATTATTCAG
    AAAGCAATAT CTGGTTTTAG GAAATGTGCA ATGATATCTG CTCACTATGG TCTCAGTGAT
    GTTTTTGACA ATCTCATAAT TTCCTTGTGC AAGTTTACAA CACTTAGCAG TGAAGCCGTT
    GAAAATCTTC CCACTGTTTT TGGAAGCAAC CTAAAAGCTC AGATTGCTGC AAAGACTGTG
    TTCCACTTAT CCCACAGACA TGGTGACATC CTTCGAGAGG GATGGAAAAA TATTATGGAC
    TCCATGCTGC AGCTTTTTAG AGCTGAGCTG CTTCCCAAGG CTATGGTTGA GGTTGAAGAT
    TTTGTAGACC CCAATGGAAA AATATCTCTT CAACGTGAGG AAATCCCTGC CAACCGTGGG
    GAATCCACTG TACTAAGTTT TGTCAGCTGG CTGACTCTGA GTGGGACAGA ACAGTCAAGT
    CTAAGAGGAC CTTCTACTGA AAATCAAGAG GCCAAGAAAC TAGCGCTGGA ATGTATCAAG
    TTATGTGATC CAGAGAAACT GATCACAGAG AGCAAGTTTC TTCAACTGGA GTCTTTGCAA
    GAACTAATGA AGGCTCTGAT ATCTGTGACA CCAGATGAAG AAACGTATGA TGAAGAAGAT
    GCTGCATTCT GCCTAGAAAT GTTAATGAGA ATTGTGCTAG AAAACAGAGA TCGTGTTGGC
    TGTGTTTGGC AGGCTGTACG AGATCATCTC TATCGTCTAT GTGTCCATGC TGTAGAGTTC
    TGCTTCCTGG TTGAAAGAGC AGTTGTAGGG CTGCTGCGCT TGGCAATACG CCTTTTACGC
    AGAGAGGAAA TTAGTGGTCA GGTTATCCTC TCCTTGAGAA TTCTTTTAAT GATGAAACCA
    ACTGTGTTGT CTAAAGTGAG TCGCCAAGTT GCTTTTGGTG TGCATGAGCT CTTGAAAACC
    AATGCTGCTA ACATCCATTC CAGCAATGAC TGGTACACAC TATTCTGCCT TTTGGAGTGC
    ATTGGGGCAG GAGTGAAGCC GCCCCCTGCC TTACAGGTGA CGACGAGAGC TGACAATGAC
    ACAGGTGCGC AGTCTGACAG TGAAGTTTCC TCATACCATC CAAGTGAAGT GAATCTTGAT
    CGTGGATACA CCTCAGACTC TGAGGTATAT GCAGAACACA GCAAGCAGTC AAAAATGCAC
    CGCTCTGTGA CTGATGTGGA TATGATGAAC AGTGGCTGGC TGATGGTTGG AAAGGAAGAT
    GTTGACTCTT CTAAAGTTGC TACAGTTATT AACAAACCTG TTGCTAATGC CCCAGTGAAT
    CAATACAGCC TAACCATTGG TTTGGATTTT GGCCCTCACG ATACCAAATC TCTCATGAAG
    AGCGTGGAGT CCCTTTCTTT TATTGTTCGA GATGCTGCAC ATGTCACACC AGAAAACTTT
    GAACTCTGTG TTAAAACAAT CCGTGTCTTT GTAGAGGCAA GTCTAAATGG AGGTGTGTGG
    TACAAATCTC AGGAGAAAAG GGTAAAGAAC CACAAATATG ACAGTAAATC CAACCGCTTC
    AAAAAGAAAA CCAAGGAAAA AGAAAATTCT GTCCGCCGTA CACGGGCTTC AAATCAGCGA
    CAATACCGAT CTCACAGTGA TGATGAGGAA GATGAAAGTG TACCAGCCAG CTATCACACT
    GTGTCATTAC AGGTTAGTCA TGATCTTTTG GACCTAATGC ACACACTTCA CACAAGAGCT
    GCCACCATAT ACAGTTCTTG GGCAGAGGAA CAGCGCAACC TAGAGACAGC TGCTGAGAAA
    ATTACAGCCG ATTCTAAGAC ACTGTGGTCA AACTGTTGGT GTCCTTTACT GCAAGGTATT
    GCTTGGCTCT GCTGTGATGC TCGGCGCCAG GTCAGAATGC AGGCTCTCAC CTACCTGCAA
    AGGGCACTTT TAGTCCATGA CTTACAGGCT CTTGATGCAC TTGAATGGGA ATCATGCTTT
    AATAAGGTGC TATTTCCATT GCTGACCAAA CTATTGGAAA ATATTAGTCC AGCTGATGTT
    GGCGGGATGG AGGAGACCAG AATGAGAGGC TCCACTCTGC TCTCAAAGGT ATTTCTACAG
    CATCTGTCTC CTCTTCTTTC TCTGTCGACA TTTGCTGCCT TGTGGCTTAC TATTCTTGAT
    TTCATGGACA AGTACATGCA TGCTGGCTCC AGTGACTTAC TGCCTGAGGC AATTCCAGAG
    TCACTGAAAA ATATGCTTCT CGTAATGGAC ACTGCCGGAA TATTCCACAG TGCAGACTCG
    CGGACGGGCT ACTCTGATTT ATGGGAGATC ACGTGGGAAA GGATAGATTG CTTTTTACCT
    CGTCTAAGGG AAGAACTGTT TAAACAAACT GTGATACAAG ATCCACCCAA TATAGTTACA
    ACAGAAACTC CTGTTGAGCC AAGACCTCCC CCTCAATCAA CTCAGTCAGA GAGACCAGCG
    GAGCAGGGAC CTTGTGACTC AGAGACTCAA AGTTTAGCAA TCTCATCTGC TCCAGTAGTT
    GGATCAAGTT CTTCCTGCAC CCCAGTTAGA GTCAGCCCTG TAACAGATAT ACCTCCACCT
    ATAACTCAGT CTCCAGTCAT CCTGCATCCT CTGACCTCCC CGCTGCAGGT GGGAGTGCCA
    CCCATGTCGC TGCCAATCAT CCTCAACCCT GCATTAATTG AAGCTACCTC TCCAGTTCCG
    CTCTTGTCCA CTCCCCGACC TGCTGATGTA TCATCTGTCT CAGAGGTCAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012822308.1
    CDS35..5608
    Translation

    Target ORF information:

    RefSeq Version XM_012966854.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966854.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGT TGAAAATGAG AGCTGGAGGA
    ATGAGTGACT CCTCAAAATG GAAAAAGCAG AAGAAATCTC CTAGGCAGCC ACGTCATGTT
    ACCAAAGCTA TGGCGGCAGC AGAACCCTCC TCTCAGCAAC CAGTAGTTAA TGGCACCACA
    ATTACTACTA ACCTCACTGG TGAAGTTCCT TTTATTGATG CTTCCTCTCC AGATGCTAAT
    TTTCCCATTT CAGGGGTGAG TTCAGAGGCC ACGTCTGCTG TAGTCAGTCC TACCACAGAT
    AGTGGTGTAG ATATGTCCTC TCAGGCAACA TCTAAAGAAG ACCTTTCTGA ACTGGATCCT
    GCAAGTCCAT CTCTTTCTGC CATTCAGCAA TCAGGAGACA TTTGTCTTTC TGAACAGCAA
    AACCAGGATG ATGATAAGAT TGAAGACCTT ATGGAAAGGC ATACATATTC TGCTTCTGTG
    GAATCCATAC CAGAAGTATT AGATGATAAT GTGTCATTGG CTGATCAATC AGACTCCGCT
    TCAGTCCATG ATATGGATTA TGTAAATCCT AGAGGTGTGA GATTTACCCA ATCTTCACAG
    AAAGAAGGAA CAGTCTTAGT ACCATATGGA CTGCCATGCA TTCGGGAATT ATTTCGTTTT
    CTTATTTCTT TAACCAACCC CCATGATCGA CATAACTCCG AAGTGATGAT ACACATGGGT
    TTACAGTTGA TCACAGTTGC TCTTGAGTCT GCACCTATTG AAAATTATGT TTCGCTTTTA
    GGCCTTGTAA AGGATGAACT TTGTCGACAC TTATTTCAGC TACTGAGCAT TGAGAGACTG
    AACCTATATG CAGCTTCCTT GCGAGCATGT TTTCTTTTGT TTGAGGGTAT GAGGGAGCAT
    TTGAAGTTCC AGCTAGAGAT GTACATAAAG AAGCTCATGG ATATTATTAC TGTGGAGAAC
    CCTAAGATGC CTTATGAGAT GAAAGAAATG GCTCTTGAAG CTATTGTGCA ATTGTGGAGA
    ATCCCGAGCT TTGTTACAGA ATTATACATT AATTACGACT GTGATTATTA TTGCTCCAAC
    CTTTTTGAAG ATCTCACTAA ACTGCTTTCC AAGAATGCCT TTCCAGTTTC TGGACAGCTG
    TACACCACTC ATCTCCTTTC CCTGGAAGCT CTGCTAACTG TCATTGATAG CACAGAATCA
    CACTGCCAGG CTAAAAATCT AAGCAATGTG TCACAGGATA AGAAAGAAAC TGGAAAACCA
    AGTGGAGATC TGTCAGAAGG GACCAAAGAT TCTGGAAATC TCAATGAACA CTCCATTGAA
    GTTAAGCCAT TCACTGTTAG TGCAGAAAAC AATGGATCGA AGCCCCCCAC AAGTGGCCAC
    TTAATGGCAG ATAAAATGAC TCTTGGGGTC CAAGAAACAG AGTACAGCAC TGATGGAGGT
    GAGAAGAAGA CTTTAAAGAA GCCCCAACGC TTTTCCTGCT TACTTCCTAA TGTTCAAGAG
    CTGAATGACA TTAAGACCAA AAAGAAGCTT CTTATAACTG GAACTGAGCA GTTCAACCAG
    AAACCAAAGA AAGGAGTGCA GTTTCTTCAG GAAAAAGACC TTTTGGCAAC CCCCATGGAT
    AATGCTGAGG TAGCACAGTG GCTCAGAGAA AATCCAAGGC TGGACAAGAA AATGATTGGG
    GAGTTTGTGA GTGATCGCAA AAATCTGGAC TTACTTGAAA GTTTTGTAGG AACCTTCCAT
    TTCCAAGGCC TGAGAGTTGA TGAAGCTTTA AGGCTTTACC TTGAAGCTTT TCGTTTGCCT
    GGCGAGGCAC CTGTGATTCA GAGGTTACTG GAGGCTTTTA CAGAGCACTG GCGAAAATCC
    AATGGAACTC CTTTTGCACA CAGTGATGCC TGCTTTGCTT TGGCTTATGC AGTTATCATG
    CTGAACACAG ACCAACACAA TCACAATGTC CGCAAGCAAA ATGTGCCAAT GACTTTAGAG
    GAATTCAGGA AAAATTTAAA GGGTGTTAAT GGTGGAAAAG ATTTTGACCA AGATATGCTA
    GAGGATATTT ATCACGCAAT CAAAAATGAA GAAATTGTAA TGCCTGAAGA GCAGACTGGC
    TTAGTAAAAG AGAATTATTT TTGGAATGTT TTGCTGCATC GGGGAGCCAC TCCAGAAGGA
    ATGTTCTTGC ATGTGGATCC TGGAAGTTAT GACCATGACC TCTTTACTAT GACTTGGGGG
    CCAACAATAG CTGCCTTGTC CTATGTGTTT GATAAGAGCA TGGATGAAAC GATTATTCAG
    AAAGCAATAT CTGGTTTTAG GAAATGTGCA ATGATATCTG CTCACTATGG TCTCAGTGAT
    GTTTTTGACA ATCTCATAAT TTCCTTGTGC AAGTTTACAA CACTTAGCAG TGAAGCCGTT
    GAAAATCTTC CCACTGTTTT TGGAAGCAAC CTAAAAGCTC AGATTGCTGC AAAGACTGTG
    TTCCACTTAT CCCACAGACA TGGTGACATC CTTCGAGAGG GATGGAAAAA TATTATGGAC
    TCCATGCTGC AGCTTTTTAG AGCTGAGCTG CTTCCCAAGG CTATGGTTGA GGTTGAAGAT
    TTTGTAGACC CCAATGGAAA AATATCTCTT CAACGTGAGG AAATCCCTGC CAACCGTGGG
    GAATCCACTG TACTAAGTTT TGTCAGCTGG CTGACTCTGA GTGGGACAGA ACAGTCAAGT
    CTAAGAGGAC CTTCTACTGA AAATCAAGAG GCCAAGAAAC TAGCGCTGGA ATGTATCAAG
    TTATGTGATC CAGAGAAACT GATCACAGAG AGCAAGTTTC TTCAACTGGA GTCTTTGCAA
    GAACTAATGA AGGCTCTGAT ATCTGTGACA CCAGATGAAG AAACGTATGA TGAAGAAGAT
    GCTGCATTCT GCCTAGAAAT GTTAATGAGA ATTGTGCTAG AAAACAGAGA TCGTGTTGGC
    TGTGTTTGGC AGGCTGTACG AGATCATCTC TATCGTCTAT GTGTCCATGC TGTAGAGTTC
    TGCTTCCTGG TTGAAAGAGC AGTTGTAGGG CTGCTGCGCT TGGCAATACG CCTTTTACGC
    AGAGAGGAAA TTAGTGGTCA GGTTATCCTC TCCTTGAGAA TTCTTTTAAT GATGAAACCA
    ACTGTGTTGT CTAAAGTGAG TCGCCAAGTT GCTTTTGGTG TGCATGAGCT CTTGAAAACC
    AATGCTGCTA ACATCCATTC CAGCAATGAC TGGTACACAC TATTCTGCCT TTTGGAGTGC
    ATTGGGGCAG GAGTGAAGCC GCCCCCTGCC TTACAGGTGA CGACGAGAGC TGACAATGAC
    ACAGGTGCGC AGTCTGACAG TGAAGTTTCC TCATACCATC CAAGTGAAGT GAATCTTGAT
    CGTGGATACA CCTCAGACTC TGAGGTATAT GCAGAACACA GCAAGCAGTC AAAAATGCAC
    CGCTCTGTGA CTGATGTGGA TATGATGAAC AGTGGCTGGC TGATGGTTGG AAAGGAAGAT
    GTTGACTCTT CTAAAGTTGC TACAGTTATT AACAAACCTG TTGCTAATGC CCCAGTGAAT
    CAATACAGCC TAACCATTGG TTTGGATTTT GGCCCTCACG ATACCAAATC TCTCATGAAG
    AGCGTGGAGT CCCTTTCTTT TATTGTTCGA GATGCTGCAC ATGTCACACC AGAAAACTTT
    GAACTCTGTG TTAAAACAAT CCGTGTCTTT GTAGAGGCAA GTCTAAATGG AGGTGTGTGG
    TACAAATCTC AGGAGAAAAG GGTAAAGAAC CACAAATATG ACAGTAAATC CAACCGCTTC
    AAAAAGAAAA CCAAGGAAAA AGAAAATTCT GTCCGCCGTA CACGGGCTTC AAATCAGCGA
    CAATACCGAT CTCACAGTGA TGATGAGGAA GATGAAAGTG TACCAGCCAG CTATCACACT
    GTGTCATTAC AGGTTAGTCA TGATCTTTTG GACCTAATGC ACACACTTCA CACAAGAGCT
    GCCACCATAT ACAGTTCTTG GGCAGAGGAA CAGCGCAACC TAGAGACAGC TGCTGAGAAA
    ATTACAGCCG ATTCTAAGAC ACTGTGGTCA AACTGTTGGT GTCCTTTACT GCAAGGTATT
    GCTTGGCTCT GCTGTGATGC TCGGCGCCAG GTCAGAATGC AGGCTCTCAC CTACCTGCAA
    AGGGCACTTT TAGTCCATGA CTTACAGGCT CTTGATGCAC TTGAATGGGA ATCATGCTTT
    AATAAGGTGC TATTTCCATT GCTGACCAAA CTATTGGAAA ATATTAGTCC AGCTGATGTT
    GGCGGGATGG AGGAGACCAG AATGAGAGGC TCCACTCTGC TCTCAAAGGT ATTTCTACAG
    CATCTGTCTC CTCTTCTTTC TCTGTCGACA TTTGCTGCCT TGTGGCTTAC TATTCTTGAT
    TTCATGGACA AGTACATGCA TGCTGGCTCC AGTGACTTAC TGCCTGAGGC AATTCCAGAG
    TCACTGAAAA ATATGCTTCT CGTAATGGAC ACTGCCGGAA TATTCCACAG TGCAGACTCG
    CGGACGGGCT ACTCTGATTT ATGGGAGATC ACGTGGGAAA GGATAGATTG CTTTTTACCT
    CGTCTAAGGG AAGAACTGTT TAAACAAACT GTGATACAAG ATCCACCCAA TATAGTTACA
    ACAGAAACTC CTGTTGAGCC AAGACCTCCC CCTCAATCAA CTCAGTCAGA GAGACCAGCG
    GAGCAGGGAC CTTGTGACTC AGAGACTCAA AGTTTAGCAA TCTCATCTGC TCCAGTAGTT
    GGATCAAGTT CTTCCTGCAC CCCAGTTAGA GTCAGCCCTG TAACAGATAT ACCTCCACCT
    ATAACTCAGT CTCCAGTCAT CCTGCATCCT CTGACCTCCC CGCTGCAGGT GGGAGTGCCA
    CCCATGTCGC TGCCAATCAT CCTCAACCCT GCATTAATTG AAGCTACCTC TCCAGTTCCG
    CTCTTGTCCA CTCCCCGACC TGCTGATGTA TCATCTGTCT CAGAGGTCAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa33097
    Clone ID Related Accession (Same CDS sequence) XM_012966852.2 , XM_012966854.2 , XM_012966853.2 , XM_012966851.2
    Accession Version XM_012966853.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5574bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966853.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGT TGAAAATGAG AGCTGGAGGA
    ATGAGTGACT CCTCAAAATG GAAAAAGCAG AAGAAATCTC CTAGGCAGCC ACGTCATGTT
    ACCAAAGCTA TGGCGGCAGC AGAACCCTCC TCTCAGCAAC CAGTAGTTAA TGGCACCACA
    ATTACTACTA ACCTCACTGG TGAAGTTCCT TTTATTGATG CTTCCTCTCC AGATGCTAAT
    TTTCCCATTT CAGGGGTGAG TTCAGAGGCC ACGTCTGCTG TAGTCAGTCC TACCACAGAT
    AGTGGTGTAG ATATGTCCTC TCAGGCAACA TCTAAAGAAG ACCTTTCTGA ACTGGATCCT
    GCAAGTCCAT CTCTTTCTGC CATTCAGCAA TCAGGAGACA TTTGTCTTTC TGAACAGCAA
    AACCAGGATG ATGATAAGAT TGAAGACCTT ATGGAAAGGC ATACATATTC TGCTTCTGTG
    GAATCCATAC CAGAAGTATT AGATGATAAT GTGTCATTGG CTGATCAATC AGACTCCGCT
    TCAGTCCATG ATATGGATTA TGTAAATCCT AGAGGTGTGA GATTTACCCA ATCTTCACAG
    AAAGAAGGAA CAGTCTTAGT ACCATATGGA CTGCCATGCA TTCGGGAATT ATTTCGTTTT
    CTTATTTCTT TAACCAACCC CCATGATCGA CATAACTCCG AAGTGATGAT ACACATGGGT
    TTACAGTTGA TCACAGTTGC TCTTGAGTCT GCACCTATTG AAAATTATGT TTCGCTTTTA
    GGCCTTGTAA AGGATGAACT TTGTCGACAC TTATTTCAGC TACTGAGCAT TGAGAGACTG
    AACCTATATG CAGCTTCCTT GCGAGCATGT TTTCTTTTGT TTGAGGGTAT GAGGGAGCAT
    TTGAAGTTCC AGCTAGAGAT GTACATAAAG AAGCTCATGG ATATTATTAC TGTGGAGAAC
    CCTAAGATGC CTTATGAGAT GAAAGAAATG GCTCTTGAAG CTATTGTGCA ATTGTGGAGA
    ATCCCGAGCT TTGTTACAGA ATTATACATT AATTACGACT GTGATTATTA TTGCTCCAAC
    CTTTTTGAAG ATCTCACTAA ACTGCTTTCC AAGAATGCCT TTCCAGTTTC TGGACAGCTG
    TACACCACTC ATCTCCTTTC CCTGGAAGCT CTGCTAACTG TCATTGATAG CACAGAATCA
    CACTGCCAGG CTAAAAATCT AAGCAATGTG TCACAGGATA AGAAAGAAAC TGGAAAACCA
    AGTGGAGATC TGTCAGAAGG GACCAAAGAT TCTGGAAATC TCAATGAACA CTCCATTGAA
    GTTAAGCCAT TCACTGTTAG TGCAGAAAAC AATGGATCGA AGCCCCCCAC AAGTGGCCAC
    TTAATGGCAG ATAAAATGAC TCTTGGGGTC CAAGAAACAG AGTACAGCAC TGATGGAGGT
    GAGAAGAAGA CTTTAAAGAA GCCCCAACGC TTTTCCTGCT TACTTCCTAA TGTTCAAGAG
    CTGAATGACA TTAAGACCAA AAAGAAGCTT CTTATAACTG GAACTGAGCA GTTCAACCAG
    AAACCAAAGA AAGGAGTGCA GTTTCTTCAG GAAAAAGACC TTTTGGCAAC CCCCATGGAT
    AATGCTGAGG TAGCACAGTG GCTCAGAGAA AATCCAAGGC TGGACAAGAA AATGATTGGG
    GAGTTTGTGA GTGATCGCAA AAATCTGGAC TTACTTGAAA GTTTTGTAGG AACCTTCCAT
    TTCCAAGGCC TGAGAGTTGA TGAAGCTTTA AGGCTTTACC TTGAAGCTTT TCGTTTGCCT
    GGCGAGGCAC CTGTGATTCA GAGGTTACTG GAGGCTTTTA CAGAGCACTG GCGAAAATCC
    AATGGAACTC CTTTTGCACA CAGTGATGCC TGCTTTGCTT TGGCTTATGC AGTTATCATG
    CTGAACACAG ACCAACACAA TCACAATGTC CGCAAGCAAA ATGTGCCAAT GACTTTAGAG
    GAATTCAGGA AAAATTTAAA GGGTGTTAAT GGTGGAAAAG ATTTTGACCA AGATATGCTA
    GAGGATATTT ATCACGCAAT CAAAAATGAA GAAATTGTAA TGCCTGAAGA GCAGACTGGC
    TTAGTAAAAG AGAATTATTT TTGGAATGTT TTGCTGCATC GGGGAGCCAC TCCAGAAGGA
    ATGTTCTTGC ATGTGGATCC TGGAAGTTAT GACCATGACC TCTTTACTAT GACTTGGGGG
    CCAACAATAG CTGCCTTGTC CTATGTGTTT GATAAGAGCA TGGATGAAAC GATTATTCAG
    AAAGCAATAT CTGGTTTTAG GAAATGTGCA ATGATATCTG CTCACTATGG TCTCAGTGAT
    GTTTTTGACA ATCTCATAAT TTCCTTGTGC AAGTTTACAA CACTTAGCAG TGAAGCCGTT
    GAAAATCTTC CCACTGTTTT TGGAAGCAAC CTAAAAGCTC AGATTGCTGC AAAGACTGTG
    TTCCACTTAT CCCACAGACA TGGTGACATC CTTCGAGAGG GATGGAAAAA TATTATGGAC
    TCCATGCTGC AGCTTTTTAG AGCTGAGCTG CTTCCCAAGG CTATGGTTGA GGTTGAAGAT
    TTTGTAGACC CCAATGGAAA AATATCTCTT CAACGTGAGG AAATCCCTGC CAACCGTGGG
    GAATCCACTG TACTAAGTTT TGTCAGCTGG CTGACTCTGA GTGGGACAGA ACAGTCAAGT
    CTAAGAGGAC CTTCTACTGA AAATCAAGAG GCCAAGAAAC TAGCGCTGGA ATGTATCAAG
    TTATGTGATC CAGAGAAACT GATCACAGAG AGCAAGTTTC TTCAACTGGA GTCTTTGCAA
    GAACTAATGA AGGCTCTGAT ATCTGTGACA CCAGATGAAG AAACGTATGA TGAAGAAGAT
    GCTGCATTCT GCCTAGAAAT GTTAATGAGA ATTGTGCTAG AAAACAGAGA TCGTGTTGGC
    TGTGTTTGGC AGGCTGTACG AGATCATCTC TATCGTCTAT GTGTCCATGC TGTAGAGTTC
    TGCTTCCTGG TTGAAAGAGC AGTTGTAGGG CTGCTGCGCT TGGCAATACG CCTTTTACGC
    AGAGAGGAAA TTAGTGGTCA GGTTATCCTC TCCTTGAGAA TTCTTTTAAT GATGAAACCA
    ACTGTGTTGT CTAAAGTGAG TCGCCAAGTT GCTTTTGGTG TGCATGAGCT CTTGAAAACC
    AATGCTGCTA ACATCCATTC CAGCAATGAC TGGTACACAC TATTCTGCCT TTTGGAGTGC
    ATTGGGGCAG GAGTGAAGCC GCCCCCTGCC TTACAGGTGA CGACGAGAGC TGACAATGAC
    ACAGGTGCGC AGTCTGACAG TGAAGTTTCC TCATACCATC CAAGTGAAGT GAATCTTGAT
    CGTGGATACA CCTCAGACTC TGAGGTATAT GCAGAACACA GCAAGCAGTC AAAAATGCAC
    CGCTCTGTGA CTGATGTGGA TATGATGAAC AGTGGCTGGC TGATGGTTGG AAAGGAAGAT
    GTTGACTCTT CTAAAGTTGC TACAGTTATT AACAAACCTG TTGCTAATGC CCCAGTGAAT
    CAATACAGCC TAACCATTGG TTTGGATTTT GGCCCTCACG ATACCAAATC TCTCATGAAG
    AGCGTGGAGT CCCTTTCTTT TATTGTTCGA GATGCTGCAC ATGTCACACC AGAAAACTTT
    GAACTCTGTG TTAAAACAAT CCGTGTCTTT GTAGAGGCAA GTCTAAATGG AGGTGTGTGG
    TACAAATCTC AGGAGAAAAG GGTAAAGAAC CACAAATATG ACAGTAAATC CAACCGCTTC
    AAAAAGAAAA CCAAGGAAAA AGAAAATTCT GTCCGCCGTA CACGGGCTTC AAATCAGCGA
    CAATACCGAT CTCACAGTGA TGATGAGGAA GATGAAAGTG TACCAGCCAG CTATCACACT
    GTGTCATTAC AGGTTAGTCA TGATCTTTTG GACCTAATGC ACACACTTCA CACAAGAGCT
    GCCACCATAT ACAGTTCTTG GGCAGAGGAA CAGCGCAACC TAGAGACAGC TGCTGAGAAA
    ATTACAGCCG ATTCTAAGAC ACTGTGGTCA AACTGTTGGT GTCCTTTACT GCAAGGTATT
    GCTTGGCTCT GCTGTGATGC TCGGCGCCAG GTCAGAATGC AGGCTCTCAC CTACCTGCAA
    AGGGCACTTT TAGTCCATGA CTTACAGGCT CTTGATGCAC TTGAATGGGA ATCATGCTTT
    AATAAGGTGC TATTTCCATT GCTGACCAAA CTATTGGAAA ATATTAGTCC AGCTGATGTT
    GGCGGGATGG AGGAGACCAG AATGAGAGGC TCCACTCTGC TCTCAAAGGT ATTTCTACAG
    CATCTGTCTC CTCTTCTTTC TCTGTCGACA TTTGCTGCCT TGTGGCTTAC TATTCTTGAT
    TTCATGGACA AGTACATGCA TGCTGGCTCC AGTGACTTAC TGCCTGAGGC AATTCCAGAG
    TCACTGAAAA ATATGCTTCT CGTAATGGAC ACTGCCGGAA TATTCCACAG TGCAGACTCG
    CGGACGGGCT ACTCTGATTT ATGGGAGATC ACGTGGGAAA GGATAGATTG CTTTTTACCT
    CGTCTAAGGG AAGAACTGTT TAAACAAACT GTGATACAAG ATCCACCCAA TATAGTTACA
    ACAGAAACTC CTGTTGAGCC AAGACCTCCC CCTCAATCAA CTCAGTCAGA GAGACCAGCG
    GAGCAGGGAC CTTGTGACTC AGAGACTCAA AGTTTAGCAA TCTCATCTGC TCCAGTAGTT
    GGATCAAGTT CTTCCTGCAC CCCAGTTAGA GTCAGCCCTG TAACAGATAT ACCTCCACCT
    ATAACTCAGT CTCCAGTCAT CCTGCATCCT CTGACCTCCC CGCTGCAGGT GGGAGTGCCA
    CCCATGTCGC TGCCAATCAT CCTCAACCCT GCATTAATTG AAGCTACCTC TCCAGTTCCG
    CTCTTGTCCA CTCCCCGACC TGCTGATGTA TCATCTGTCT CAGAGGTCAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012822307.1
    CDS84..5657
    Translation

    Target ORF information:

    RefSeq Version XM_012966853.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966853.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGT TGAAAATGAG AGCTGGAGGA
    ATGAGTGACT CCTCAAAATG GAAAAAGCAG AAGAAATCTC CTAGGCAGCC ACGTCATGTT
    ACCAAAGCTA TGGCGGCAGC AGAACCCTCC TCTCAGCAAC CAGTAGTTAA TGGCACCACA
    ATTACTACTA ACCTCACTGG TGAAGTTCCT TTTATTGATG CTTCCTCTCC AGATGCTAAT
    TTTCCCATTT CAGGGGTGAG TTCAGAGGCC ACGTCTGCTG TAGTCAGTCC TACCACAGAT
    AGTGGTGTAG ATATGTCCTC TCAGGCAACA TCTAAAGAAG ACCTTTCTGA ACTGGATCCT
    GCAAGTCCAT CTCTTTCTGC CATTCAGCAA TCAGGAGACA TTTGTCTTTC TGAACAGCAA
    AACCAGGATG ATGATAAGAT TGAAGACCTT ATGGAAAGGC ATACATATTC TGCTTCTGTG
    GAATCCATAC CAGAAGTATT AGATGATAAT GTGTCATTGG CTGATCAATC AGACTCCGCT
    TCAGTCCATG ATATGGATTA TGTAAATCCT AGAGGTGTGA GATTTACCCA ATCTTCACAG
    AAAGAAGGAA CAGTCTTAGT ACCATATGGA CTGCCATGCA TTCGGGAATT ATTTCGTTTT
    CTTATTTCTT TAACCAACCC CCATGATCGA CATAACTCCG AAGTGATGAT ACACATGGGT
    TTACAGTTGA TCACAGTTGC TCTTGAGTCT GCACCTATTG AAAATTATGT TTCGCTTTTA
    GGCCTTGTAA AGGATGAACT TTGTCGACAC TTATTTCAGC TACTGAGCAT TGAGAGACTG
    AACCTATATG CAGCTTCCTT GCGAGCATGT TTTCTTTTGT TTGAGGGTAT GAGGGAGCAT
    TTGAAGTTCC AGCTAGAGAT GTACATAAAG AAGCTCATGG ATATTATTAC TGTGGAGAAC
    CCTAAGATGC CTTATGAGAT GAAAGAAATG GCTCTTGAAG CTATTGTGCA ATTGTGGAGA
    ATCCCGAGCT TTGTTACAGA ATTATACATT AATTACGACT GTGATTATTA TTGCTCCAAC
    CTTTTTGAAG ATCTCACTAA ACTGCTTTCC AAGAATGCCT TTCCAGTTTC TGGACAGCTG
    TACACCACTC ATCTCCTTTC CCTGGAAGCT CTGCTAACTG TCATTGATAG CACAGAATCA
    CACTGCCAGG CTAAAAATCT AAGCAATGTG TCACAGGATA AGAAAGAAAC TGGAAAACCA
    AGTGGAGATC TGTCAGAAGG GACCAAAGAT TCTGGAAATC TCAATGAACA CTCCATTGAA
    GTTAAGCCAT TCACTGTTAG TGCAGAAAAC AATGGATCGA AGCCCCCCAC AAGTGGCCAC
    TTAATGGCAG ATAAAATGAC TCTTGGGGTC CAAGAAACAG AGTACAGCAC TGATGGAGGT
    GAGAAGAAGA CTTTAAAGAA GCCCCAACGC TTTTCCTGCT TACTTCCTAA TGTTCAAGAG
    CTGAATGACA TTAAGACCAA AAAGAAGCTT CTTATAACTG GAACTGAGCA GTTCAACCAG
    AAACCAAAGA AAGGAGTGCA GTTTCTTCAG GAAAAAGACC TTTTGGCAAC CCCCATGGAT
    AATGCTGAGG TAGCACAGTG GCTCAGAGAA AATCCAAGGC TGGACAAGAA AATGATTGGG
    GAGTTTGTGA GTGATCGCAA AAATCTGGAC TTACTTGAAA GTTTTGTAGG AACCTTCCAT
    TTCCAAGGCC TGAGAGTTGA TGAAGCTTTA AGGCTTTACC TTGAAGCTTT TCGTTTGCCT
    GGCGAGGCAC CTGTGATTCA GAGGTTACTG GAGGCTTTTA CAGAGCACTG GCGAAAATCC
    AATGGAACTC CTTTTGCACA CAGTGATGCC TGCTTTGCTT TGGCTTATGC AGTTATCATG
    CTGAACACAG ACCAACACAA TCACAATGTC CGCAAGCAAA ATGTGCCAAT GACTTTAGAG
    GAATTCAGGA AAAATTTAAA GGGTGTTAAT GGTGGAAAAG ATTTTGACCA AGATATGCTA
    GAGGATATTT ATCACGCAAT CAAAAATGAA GAAATTGTAA TGCCTGAAGA GCAGACTGGC
    TTAGTAAAAG AGAATTATTT TTGGAATGTT TTGCTGCATC GGGGAGCCAC TCCAGAAGGA
    ATGTTCTTGC ATGTGGATCC TGGAAGTTAT GACCATGACC TCTTTACTAT GACTTGGGGG
    CCAACAATAG CTGCCTTGTC CTATGTGTTT GATAAGAGCA TGGATGAAAC GATTATTCAG
    AAAGCAATAT CTGGTTTTAG GAAATGTGCA ATGATATCTG CTCACTATGG TCTCAGTGAT
    GTTTTTGACA ATCTCATAAT TTCCTTGTGC AAGTTTACAA CACTTAGCAG TGAAGCCGTT
    GAAAATCTTC CCACTGTTTT TGGAAGCAAC CTAAAAGCTC AGATTGCTGC AAAGACTGTG
    TTCCACTTAT CCCACAGACA TGGTGACATC CTTCGAGAGG GATGGAAAAA TATTATGGAC
    TCCATGCTGC AGCTTTTTAG AGCTGAGCTG CTTCCCAAGG CTATGGTTGA GGTTGAAGAT
    TTTGTAGACC CCAATGGAAA AATATCTCTT CAACGTGAGG AAATCCCTGC CAACCGTGGG
    GAATCCACTG TACTAAGTTT TGTCAGCTGG CTGACTCTGA GTGGGACAGA ACAGTCAAGT
    CTAAGAGGAC CTTCTACTGA AAATCAAGAG GCCAAGAAAC TAGCGCTGGA ATGTATCAAG
    TTATGTGATC CAGAGAAACT GATCACAGAG AGCAAGTTTC TTCAACTGGA GTCTTTGCAA
    GAACTAATGA AGGCTCTGAT ATCTGTGACA CCAGATGAAG AAACGTATGA TGAAGAAGAT
    GCTGCATTCT GCCTAGAAAT GTTAATGAGA ATTGTGCTAG AAAACAGAGA TCGTGTTGGC
    TGTGTTTGGC AGGCTGTACG AGATCATCTC TATCGTCTAT GTGTCCATGC TGTAGAGTTC
    TGCTTCCTGG TTGAAAGAGC AGTTGTAGGG CTGCTGCGCT TGGCAATACG CCTTTTACGC
    AGAGAGGAAA TTAGTGGTCA GGTTATCCTC TCCTTGAGAA TTCTTTTAAT GATGAAACCA
    ACTGTGTTGT CTAAAGTGAG TCGCCAAGTT GCTTTTGGTG TGCATGAGCT CTTGAAAACC
    AATGCTGCTA ACATCCATTC CAGCAATGAC TGGTACACAC TATTCTGCCT TTTGGAGTGC
    ATTGGGGCAG GAGTGAAGCC GCCCCCTGCC TTACAGGTGA CGACGAGAGC TGACAATGAC
    ACAGGTGCGC AGTCTGACAG TGAAGTTTCC TCATACCATC CAAGTGAAGT GAATCTTGAT
    CGTGGATACA CCTCAGACTC TGAGGTATAT GCAGAACACA GCAAGCAGTC AAAAATGCAC
    CGCTCTGTGA CTGATGTGGA TATGATGAAC AGTGGCTGGC TGATGGTTGG AAAGGAAGAT
    GTTGACTCTT CTAAAGTTGC TACAGTTATT AACAAACCTG TTGCTAATGC CCCAGTGAAT
    CAATACAGCC TAACCATTGG TTTGGATTTT GGCCCTCACG ATACCAAATC TCTCATGAAG
    AGCGTGGAGT CCCTTTCTTT TATTGTTCGA GATGCTGCAC ATGTCACACC AGAAAACTTT
    GAACTCTGTG TTAAAACAAT CCGTGTCTTT GTAGAGGCAA GTCTAAATGG AGGTGTGTGG
    TACAAATCTC AGGAGAAAAG GGTAAAGAAC CACAAATATG ACAGTAAATC CAACCGCTTC
    AAAAAGAAAA CCAAGGAAAA AGAAAATTCT GTCCGCCGTA CACGGGCTTC AAATCAGCGA
    CAATACCGAT CTCACAGTGA TGATGAGGAA GATGAAAGTG TACCAGCCAG CTATCACACT
    GTGTCATTAC AGGTTAGTCA TGATCTTTTG GACCTAATGC ACACACTTCA CACAAGAGCT
    GCCACCATAT ACAGTTCTTG GGCAGAGGAA CAGCGCAACC TAGAGACAGC TGCTGAGAAA
    ATTACAGCCG ATTCTAAGAC ACTGTGGTCA AACTGTTGGT GTCCTTTACT GCAAGGTATT
    GCTTGGCTCT GCTGTGATGC TCGGCGCCAG GTCAGAATGC AGGCTCTCAC CTACCTGCAA
    AGGGCACTTT TAGTCCATGA CTTACAGGCT CTTGATGCAC TTGAATGGGA ATCATGCTTT
    AATAAGGTGC TATTTCCATT GCTGACCAAA CTATTGGAAA ATATTAGTCC AGCTGATGTT
    GGCGGGATGG AGGAGACCAG AATGAGAGGC TCCACTCTGC TCTCAAAGGT ATTTCTACAG
    CATCTGTCTC CTCTTCTTTC TCTGTCGACA TTTGCTGCCT TGTGGCTTAC TATTCTTGAT
    TTCATGGACA AGTACATGCA TGCTGGCTCC AGTGACTTAC TGCCTGAGGC AATTCCAGAG
    TCACTGAAAA ATATGCTTCT CGTAATGGAC ACTGCCGGAA TATTCCACAG TGCAGACTCG
    CGGACGGGCT ACTCTGATTT ATGGGAGATC ACGTGGGAAA GGATAGATTG CTTTTTACCT
    CGTCTAAGGG AAGAACTGTT TAAACAAACT GTGATACAAG ATCCACCCAA TATAGTTACA
    ACAGAAACTC CTGTTGAGCC AAGACCTCCC CCTCAATCAA CTCAGTCAGA GAGACCAGCG
    GAGCAGGGAC CTTGTGACTC AGAGACTCAA AGTTTAGCAA TCTCATCTGC TCCAGTAGTT
    GGATCAAGTT CTTCCTGCAC CCCAGTTAGA GTCAGCCCTG TAACAGATAT ACCTCCACCT
    ATAACTCAGT CTCCAGTCAT CCTGCATCCT CTGACCTCCC CGCTGCAGGT GGGAGTGCCA
    CCCATGTCGC TGCCAATCAT CCTCAACCCT GCATTAATTG AAGCTACCTC TCCAGTTCCG
    CTCTTGTCCA CTCCCCGACC TGCTGATGTA TCATCTGTCT CAGAGGTCAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa33097
    Clone ID Related Accession (Same CDS sequence) XM_012966852.2 , XM_012966854.2 , XM_012966853.2 , XM_012966851.2
    Accession Version XM_012966851.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5574bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966851.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGT TGAAAATGAG AGCTGGAGGA
    ATGAGTGACT CCTCAAAATG GAAAAAGCAG AAGAAATCTC CTAGGCAGCC ACGTCATGTT
    ACCAAAGCTA TGGCGGCAGC AGAACCCTCC TCTCAGCAAC CAGTAGTTAA TGGCACCACA
    ATTACTACTA ACCTCACTGG TGAAGTTCCT TTTATTGATG CTTCCTCTCC AGATGCTAAT
    TTTCCCATTT CAGGGGTGAG TTCAGAGGCC ACGTCTGCTG TAGTCAGTCC TACCACAGAT
    AGTGGTGTAG ATATGTCCTC TCAGGCAACA TCTAAAGAAG ACCTTTCTGA ACTGGATCCT
    GCAAGTCCAT CTCTTTCTGC CATTCAGCAA TCAGGAGACA TTTGTCTTTC TGAACAGCAA
    AACCAGGATG ATGATAAGAT TGAAGACCTT ATGGAAAGGC ATACATATTC TGCTTCTGTG
    GAATCCATAC CAGAAGTATT AGATGATAAT GTGTCATTGG CTGATCAATC AGACTCCGCT
    TCAGTCCATG ATATGGATTA TGTAAATCCT AGAGGTGTGA GATTTACCCA ATCTTCACAG
    AAAGAAGGAA CAGTCTTAGT ACCATATGGA CTGCCATGCA TTCGGGAATT ATTTCGTTTT
    CTTATTTCTT TAACCAACCC CCATGATCGA CATAACTCCG AAGTGATGAT ACACATGGGT
    TTACAGTTGA TCACAGTTGC TCTTGAGTCT GCACCTATTG AAAATTATGT TTCGCTTTTA
    GGCCTTGTAA AGGATGAACT TTGTCGACAC TTATTTCAGC TACTGAGCAT TGAGAGACTG
    AACCTATATG CAGCTTCCTT GCGAGCATGT TTTCTTTTGT TTGAGGGTAT GAGGGAGCAT
    TTGAAGTTCC AGCTAGAGAT GTACATAAAG AAGCTCATGG ATATTATTAC TGTGGAGAAC
    CCTAAGATGC CTTATGAGAT GAAAGAAATG GCTCTTGAAG CTATTGTGCA ATTGTGGAGA
    ATCCCGAGCT TTGTTACAGA ATTATACATT AATTACGACT GTGATTATTA TTGCTCCAAC
    CTTTTTGAAG ATCTCACTAA ACTGCTTTCC AAGAATGCCT TTCCAGTTTC TGGACAGCTG
    TACACCACTC ATCTCCTTTC CCTGGAAGCT CTGCTAACTG TCATTGATAG CACAGAATCA
    CACTGCCAGG CTAAAAATCT AAGCAATGTG TCACAGGATA AGAAAGAAAC TGGAAAACCA
    AGTGGAGATC TGTCAGAAGG GACCAAAGAT TCTGGAAATC TCAATGAACA CTCCATTGAA
    GTTAAGCCAT TCACTGTTAG TGCAGAAAAC AATGGATCGA AGCCCCCCAC AAGTGGCCAC
    TTAATGGCAG ATAAAATGAC TCTTGGGGTC CAAGAAACAG AGTACAGCAC TGATGGAGGT
    GAGAAGAAGA CTTTAAAGAA GCCCCAACGC TTTTCCTGCT TACTTCCTAA TGTTCAAGAG
    CTGAATGACA TTAAGACCAA AAAGAAGCTT CTTATAACTG GAACTGAGCA GTTCAACCAG
    AAACCAAAGA AAGGAGTGCA GTTTCTTCAG GAAAAAGACC TTTTGGCAAC CCCCATGGAT
    AATGCTGAGG TAGCACAGTG GCTCAGAGAA AATCCAAGGC TGGACAAGAA AATGATTGGG
    GAGTTTGTGA GTGATCGCAA AAATCTGGAC TTACTTGAAA GTTTTGTAGG AACCTTCCAT
    TTCCAAGGCC TGAGAGTTGA TGAAGCTTTA AGGCTTTACC TTGAAGCTTT TCGTTTGCCT
    GGCGAGGCAC CTGTGATTCA GAGGTTACTG GAGGCTTTTA CAGAGCACTG GCGAAAATCC
    AATGGAACTC CTTTTGCACA CAGTGATGCC TGCTTTGCTT TGGCTTATGC AGTTATCATG
    CTGAACACAG ACCAACACAA TCACAATGTC CGCAAGCAAA ATGTGCCAAT GACTTTAGAG
    GAATTCAGGA AAAATTTAAA GGGTGTTAAT GGTGGAAAAG ATTTTGACCA AGATATGCTA
    GAGGATATTT ATCACGCAAT CAAAAATGAA GAAATTGTAA TGCCTGAAGA GCAGACTGGC
    TTAGTAAAAG AGAATTATTT TTGGAATGTT TTGCTGCATC GGGGAGCCAC TCCAGAAGGA
    ATGTTCTTGC ATGTGGATCC TGGAAGTTAT GACCATGACC TCTTTACTAT GACTTGGGGG
    CCAACAATAG CTGCCTTGTC CTATGTGTTT GATAAGAGCA TGGATGAAAC GATTATTCAG
    AAAGCAATAT CTGGTTTTAG GAAATGTGCA ATGATATCTG CTCACTATGG TCTCAGTGAT
    GTTTTTGACA ATCTCATAAT TTCCTTGTGC AAGTTTACAA CACTTAGCAG TGAAGCCGTT
    GAAAATCTTC CCACTGTTTT TGGAAGCAAC CTAAAAGCTC AGATTGCTGC AAAGACTGTG
    TTCCACTTAT CCCACAGACA TGGTGACATC CTTCGAGAGG GATGGAAAAA TATTATGGAC
    TCCATGCTGC AGCTTTTTAG AGCTGAGCTG CTTCCCAAGG CTATGGTTGA GGTTGAAGAT
    TTTGTAGACC CCAATGGAAA AATATCTCTT CAACGTGAGG AAATCCCTGC CAACCGTGGG
    GAATCCACTG TACTAAGTTT TGTCAGCTGG CTGACTCTGA GTGGGACAGA ACAGTCAAGT
    CTAAGAGGAC CTTCTACTGA AAATCAAGAG GCCAAGAAAC TAGCGCTGGA ATGTATCAAG
    TTATGTGATC CAGAGAAACT GATCACAGAG AGCAAGTTTC TTCAACTGGA GTCTTTGCAA
    GAACTAATGA AGGCTCTGAT ATCTGTGACA CCAGATGAAG AAACGTATGA TGAAGAAGAT
    GCTGCATTCT GCCTAGAAAT GTTAATGAGA ATTGTGCTAG AAAACAGAGA TCGTGTTGGC
    TGTGTTTGGC AGGCTGTACG AGATCATCTC TATCGTCTAT GTGTCCATGC TGTAGAGTTC
    TGCTTCCTGG TTGAAAGAGC AGTTGTAGGG CTGCTGCGCT TGGCAATACG CCTTTTACGC
    AGAGAGGAAA TTAGTGGTCA GGTTATCCTC TCCTTGAGAA TTCTTTTAAT GATGAAACCA
    ACTGTGTTGT CTAAAGTGAG TCGCCAAGTT GCTTTTGGTG TGCATGAGCT CTTGAAAACC
    AATGCTGCTA ACATCCATTC CAGCAATGAC TGGTACACAC TATTCTGCCT TTTGGAGTGC
    ATTGGGGCAG GAGTGAAGCC GCCCCCTGCC TTACAGGTGA CGACGAGAGC TGACAATGAC
    ACAGGTGCGC AGTCTGACAG TGAAGTTTCC TCATACCATC CAAGTGAAGT GAATCTTGAT
    CGTGGATACA CCTCAGACTC TGAGGTATAT GCAGAACACA GCAAGCAGTC AAAAATGCAC
    CGCTCTGTGA CTGATGTGGA TATGATGAAC AGTGGCTGGC TGATGGTTGG AAAGGAAGAT
    GTTGACTCTT CTAAAGTTGC TACAGTTATT AACAAACCTG TTGCTAATGC CCCAGTGAAT
    CAATACAGCC TAACCATTGG TTTGGATTTT GGCCCTCACG ATACCAAATC TCTCATGAAG
    AGCGTGGAGT CCCTTTCTTT TATTGTTCGA GATGCTGCAC ATGTCACACC AGAAAACTTT
    GAACTCTGTG TTAAAACAAT CCGTGTCTTT GTAGAGGCAA GTCTAAATGG AGGTGTGTGG
    TACAAATCTC AGGAGAAAAG GGTAAAGAAC CACAAATATG ACAGTAAATC CAACCGCTTC
    AAAAAGAAAA CCAAGGAAAA AGAAAATTCT GTCCGCCGTA CACGGGCTTC AAATCAGCGA
    CAATACCGAT CTCACAGTGA TGATGAGGAA GATGAAAGTG TACCAGCCAG CTATCACACT
    GTGTCATTAC AGGTTAGTCA TGATCTTTTG GACCTAATGC ACACACTTCA CACAAGAGCT
    GCCACCATAT ACAGTTCTTG GGCAGAGGAA CAGCGCAACC TAGAGACAGC TGCTGAGAAA
    ATTACAGCCG ATTCTAAGAC ACTGTGGTCA AACTGTTGGT GTCCTTTACT GCAAGGTATT
    GCTTGGCTCT GCTGTGATGC TCGGCGCCAG GTCAGAATGC AGGCTCTCAC CTACCTGCAA
    AGGGCACTTT TAGTCCATGA CTTACAGGCT CTTGATGCAC TTGAATGGGA ATCATGCTTT
    AATAAGGTGC TATTTCCATT GCTGACCAAA CTATTGGAAA ATATTAGTCC AGCTGATGTT
    GGCGGGATGG AGGAGACCAG AATGAGAGGC TCCACTCTGC TCTCAAAGGT ATTTCTACAG
    CATCTGTCTC CTCTTCTTTC TCTGTCGACA TTTGCTGCCT TGTGGCTTAC TATTCTTGAT
    TTCATGGACA AGTACATGCA TGCTGGCTCC AGTGACTTAC TGCCTGAGGC AATTCCAGAG
    TCACTGAAAA ATATGCTTCT CGTAATGGAC ACTGCCGGAA TATTCCACAG TGCAGACTCG
    CGGACGGGCT ACTCTGATTT ATGGGAGATC ACGTGGGAAA GGATAGATTG CTTTTTACCT
    CGTCTAAGGG AAGAACTGTT TAAACAAACT GTGATACAAG ATCCACCCAA TATAGTTACA
    ACAGAAACTC CTGTTGAGCC AAGACCTCCC CCTCAATCAA CTCAGTCAGA GAGACCAGCG
    GAGCAGGGAC CTTGTGACTC AGAGACTCAA AGTTTAGCAA TCTCATCTGC TCCAGTAGTT
    GGATCAAGTT CTTCCTGCAC CCCAGTTAGA GTCAGCCCTG TAACAGATAT ACCTCCACCT
    ATAACTCAGT CTCCAGTCAT CCTGCATCCT CTGACCTCCC CGCTGCAGGT GGGAGTGCCA
    CCCATGTCGC TGCCAATCAT CCTCAACCCT GCATTAATTG AAGCTACCTC TCCAGTTCCG
    CTCTTGTCCA CTCCCCGACC TGCTGATGTA TCATCTGTCT CAGAGGTCAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012822305.1
    CDS337..5910
    Translation

    Target ORF information:

    RefSeq Version XM_012966851.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966851.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGT TGAAAATGAG AGCTGGAGGA
    ATGAGTGACT CCTCAAAATG GAAAAAGCAG AAGAAATCTC CTAGGCAGCC ACGTCATGTT
    ACCAAAGCTA TGGCGGCAGC AGAACCCTCC TCTCAGCAAC CAGTAGTTAA TGGCACCACA
    ATTACTACTA ACCTCACTGG TGAAGTTCCT TTTATTGATG CTTCCTCTCC AGATGCTAAT
    TTTCCCATTT CAGGGGTGAG TTCAGAGGCC ACGTCTGCTG TAGTCAGTCC TACCACAGAT
    AGTGGTGTAG ATATGTCCTC TCAGGCAACA TCTAAAGAAG ACCTTTCTGA ACTGGATCCT
    GCAAGTCCAT CTCTTTCTGC CATTCAGCAA TCAGGAGACA TTTGTCTTTC TGAACAGCAA
    AACCAGGATG ATGATAAGAT TGAAGACCTT ATGGAAAGGC ATACATATTC TGCTTCTGTG
    GAATCCATAC CAGAAGTATT AGATGATAAT GTGTCATTGG CTGATCAATC AGACTCCGCT
    TCAGTCCATG ATATGGATTA TGTAAATCCT AGAGGTGTGA GATTTACCCA ATCTTCACAG
    AAAGAAGGAA CAGTCTTAGT ACCATATGGA CTGCCATGCA TTCGGGAATT ATTTCGTTTT
    CTTATTTCTT TAACCAACCC CCATGATCGA CATAACTCCG AAGTGATGAT ACACATGGGT
    TTACAGTTGA TCACAGTTGC TCTTGAGTCT GCACCTATTG AAAATTATGT TTCGCTTTTA
    GGCCTTGTAA AGGATGAACT TTGTCGACAC TTATTTCAGC TACTGAGCAT TGAGAGACTG
    AACCTATATG CAGCTTCCTT GCGAGCATGT TTTCTTTTGT TTGAGGGTAT GAGGGAGCAT
    TTGAAGTTCC AGCTAGAGAT GTACATAAAG AAGCTCATGG ATATTATTAC TGTGGAGAAC
    CCTAAGATGC CTTATGAGAT GAAAGAAATG GCTCTTGAAG CTATTGTGCA ATTGTGGAGA
    ATCCCGAGCT TTGTTACAGA ATTATACATT AATTACGACT GTGATTATTA TTGCTCCAAC
    CTTTTTGAAG ATCTCACTAA ACTGCTTTCC AAGAATGCCT TTCCAGTTTC TGGACAGCTG
    TACACCACTC ATCTCCTTTC CCTGGAAGCT CTGCTAACTG TCATTGATAG CACAGAATCA
    CACTGCCAGG CTAAAAATCT AAGCAATGTG TCACAGGATA AGAAAGAAAC TGGAAAACCA
    AGTGGAGATC TGTCAGAAGG GACCAAAGAT TCTGGAAATC TCAATGAACA CTCCATTGAA
    GTTAAGCCAT TCACTGTTAG TGCAGAAAAC AATGGATCGA AGCCCCCCAC AAGTGGCCAC
    TTAATGGCAG ATAAAATGAC TCTTGGGGTC CAAGAAACAG AGTACAGCAC TGATGGAGGT
    GAGAAGAAGA CTTTAAAGAA GCCCCAACGC TTTTCCTGCT TACTTCCTAA TGTTCAAGAG
    CTGAATGACA TTAAGACCAA AAAGAAGCTT CTTATAACTG GAACTGAGCA GTTCAACCAG
    AAACCAAAGA AAGGAGTGCA GTTTCTTCAG GAAAAAGACC TTTTGGCAAC CCCCATGGAT
    AATGCTGAGG TAGCACAGTG GCTCAGAGAA AATCCAAGGC TGGACAAGAA AATGATTGGG
    GAGTTTGTGA GTGATCGCAA AAATCTGGAC TTACTTGAAA GTTTTGTAGG AACCTTCCAT
    TTCCAAGGCC TGAGAGTTGA TGAAGCTTTA AGGCTTTACC TTGAAGCTTT TCGTTTGCCT
    GGCGAGGCAC CTGTGATTCA GAGGTTACTG GAGGCTTTTA CAGAGCACTG GCGAAAATCC
    AATGGAACTC CTTTTGCACA CAGTGATGCC TGCTTTGCTT TGGCTTATGC AGTTATCATG
    CTGAACACAG ACCAACACAA TCACAATGTC CGCAAGCAAA ATGTGCCAAT GACTTTAGAG
    GAATTCAGGA AAAATTTAAA GGGTGTTAAT GGTGGAAAAG ATTTTGACCA AGATATGCTA
    GAGGATATTT ATCACGCAAT CAAAAATGAA GAAATTGTAA TGCCTGAAGA GCAGACTGGC
    TTAGTAAAAG AGAATTATTT TTGGAATGTT TTGCTGCATC GGGGAGCCAC TCCAGAAGGA
    ATGTTCTTGC ATGTGGATCC TGGAAGTTAT GACCATGACC TCTTTACTAT GACTTGGGGG
    CCAACAATAG CTGCCTTGTC CTATGTGTTT GATAAGAGCA TGGATGAAAC GATTATTCAG
    AAAGCAATAT CTGGTTTTAG GAAATGTGCA ATGATATCTG CTCACTATGG TCTCAGTGAT
    GTTTTTGACA ATCTCATAAT TTCCTTGTGC AAGTTTACAA CACTTAGCAG TGAAGCCGTT
    GAAAATCTTC CCACTGTTTT TGGAAGCAAC CTAAAAGCTC AGATTGCTGC AAAGACTGTG
    TTCCACTTAT CCCACAGACA TGGTGACATC CTTCGAGAGG GATGGAAAAA TATTATGGAC
    TCCATGCTGC AGCTTTTTAG AGCTGAGCTG CTTCCCAAGG CTATGGTTGA GGTTGAAGAT
    TTTGTAGACC CCAATGGAAA AATATCTCTT CAACGTGAGG AAATCCCTGC CAACCGTGGG
    GAATCCACTG TACTAAGTTT TGTCAGCTGG CTGACTCTGA GTGGGACAGA ACAGTCAAGT
    CTAAGAGGAC CTTCTACTGA AAATCAAGAG GCCAAGAAAC TAGCGCTGGA ATGTATCAAG
    TTATGTGATC CAGAGAAACT GATCACAGAG AGCAAGTTTC TTCAACTGGA GTCTTTGCAA
    GAACTAATGA AGGCTCTGAT ATCTGTGACA CCAGATGAAG AAACGTATGA TGAAGAAGAT
    GCTGCATTCT GCCTAGAAAT GTTAATGAGA ATTGTGCTAG AAAACAGAGA TCGTGTTGGC
    TGTGTTTGGC AGGCTGTACG AGATCATCTC TATCGTCTAT GTGTCCATGC TGTAGAGTTC
    TGCTTCCTGG TTGAAAGAGC AGTTGTAGGG CTGCTGCGCT TGGCAATACG CCTTTTACGC
    AGAGAGGAAA TTAGTGGTCA GGTTATCCTC TCCTTGAGAA TTCTTTTAAT GATGAAACCA
    ACTGTGTTGT CTAAAGTGAG TCGCCAAGTT GCTTTTGGTG TGCATGAGCT CTTGAAAACC
    AATGCTGCTA ACATCCATTC CAGCAATGAC TGGTACACAC TATTCTGCCT TTTGGAGTGC
    ATTGGGGCAG GAGTGAAGCC GCCCCCTGCC TTACAGGTGA CGACGAGAGC TGACAATGAC
    ACAGGTGCGC AGTCTGACAG TGAAGTTTCC TCATACCATC CAAGTGAAGT GAATCTTGAT
    CGTGGATACA CCTCAGACTC TGAGGTATAT GCAGAACACA GCAAGCAGTC AAAAATGCAC
    CGCTCTGTGA CTGATGTGGA TATGATGAAC AGTGGCTGGC TGATGGTTGG AAAGGAAGAT
    GTTGACTCTT CTAAAGTTGC TACAGTTATT AACAAACCTG TTGCTAATGC CCCAGTGAAT
    CAATACAGCC TAACCATTGG TTTGGATTTT GGCCCTCACG ATACCAAATC TCTCATGAAG
    AGCGTGGAGT CCCTTTCTTT TATTGTTCGA GATGCTGCAC ATGTCACACC AGAAAACTTT
    GAACTCTGTG TTAAAACAAT CCGTGTCTTT GTAGAGGCAA GTCTAAATGG AGGTGTGTGG
    TACAAATCTC AGGAGAAAAG GGTAAAGAAC CACAAATATG ACAGTAAATC CAACCGCTTC
    AAAAAGAAAA CCAAGGAAAA AGAAAATTCT GTCCGCCGTA CACGGGCTTC AAATCAGCGA
    CAATACCGAT CTCACAGTGA TGATGAGGAA GATGAAAGTG TACCAGCCAG CTATCACACT
    GTGTCATTAC AGGTTAGTCA TGATCTTTTG GACCTAATGC ACACACTTCA CACAAGAGCT
    GCCACCATAT ACAGTTCTTG GGCAGAGGAA CAGCGCAACC TAGAGACAGC TGCTGAGAAA
    ATTACAGCCG ATTCTAAGAC ACTGTGGTCA AACTGTTGGT GTCCTTTACT GCAAGGTATT
    GCTTGGCTCT GCTGTGATGC TCGGCGCCAG GTCAGAATGC AGGCTCTCAC CTACCTGCAA
    AGGGCACTTT TAGTCCATGA CTTACAGGCT CTTGATGCAC TTGAATGGGA ATCATGCTTT
    AATAAGGTGC TATTTCCATT GCTGACCAAA CTATTGGAAA ATATTAGTCC AGCTGATGTT
    GGCGGGATGG AGGAGACCAG AATGAGAGGC TCCACTCTGC TCTCAAAGGT ATTTCTACAG
    CATCTGTCTC CTCTTCTTTC TCTGTCGACA TTTGCTGCCT TGTGGCTTAC TATTCTTGAT
    TTCATGGACA AGTACATGCA TGCTGGCTCC AGTGACTTAC TGCCTGAGGC AATTCCAGAG
    TCACTGAAAA ATATGCTTCT CGTAATGGAC ACTGCCGGAA TATTCCACAG TGCAGACTCG
    CGGACGGGCT ACTCTGATTT ATGGGAGATC ACGTGGGAAA GGATAGATTG CTTTTTACCT
    CGTCTAAGGG AAGAACTGTT TAAACAAACT GTGATACAAG ATCCACCCAA TATAGTTACA
    ACAGAAACTC CTGTTGAGCC AAGACCTCCC CCTCAATCAA CTCAGTCAGA GAGACCAGCG
    GAGCAGGGAC CTTGTGACTC AGAGACTCAA AGTTTAGCAA TCTCATCTGC TCCAGTAGTT
    GGATCAAGTT CTTCCTGCAC CCCAGTTAGA GTCAGCCCTG TAACAGATAT ACCTCCACCT
    ATAACTCAGT CTCCAGTCAT CCTGCATCCT CTGACCTCCC CGCTGCAGGT GGGAGTGCCA
    CCCATGTCGC TGCCAATCAT CCTCAACCCT GCATTAATTG AAGCTACCTC TCCAGTTCCG
    CTCTTGTCCA CTCCCCGACC TGCTGATGTA TCATCTGTCT CAGAGGTCAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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