cacna1i cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	cacna1i
Entrez Gene ID	100494599
Full Name	calcium channel, voltage-dependent, T type, alpha 1I subunit
Synonyms	ca(v)3.3,cav3.3
General protein information	Preferred Names calcium channel, voltage-dependent, T type, alpha 1I subunit Names voltage-dependent T-type calcium channel subunit alpha-1I
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018093215.2	XP_017948704.2	voltage-dependent T-type calcium channel subunit alpha-1I
XM_018093215.1	XP_017948704.1	voltage-dependent T-type calcium channel subunit alpha-1I

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	CACNA1I	XP_425474.3
Pan troglodytes (chimpanzee)	CACNA1I	XP_515143.4
Canis lupus familiaris (dog)	CACNA1I	XP_538364.4
Homo sapiens (human)	CACNA1I	NP_001003406.1
Bos taurus (cattle)	CACNA1I	XP_002687981.2
Mus musculus (house mouse)	Cacna1i	NP_001037773.2
Xenopus tropicalis (tropical clawed frog)	cacna1i	XP_002933803.2
Rattus norvegicus (Norway rat)	Cacna1i	NP_064469.3
Danio rerio (zebrafish)	cacna1i	XP_694715.5

Gene Ontology

The following cacna1i gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cacna1i cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OXa43932	XM_018093215.1 Latest version!	Xenopus tropicalis calcium channel, voltage-dependent, T type, alpha 1I subunit (cacna1i), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa57430	XM_018093215.2 Latest version!	Xenopus tropicalis calcium channel, voltage-dependent, T type, alpha 1I subunit (cacna1i), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cacna1i ( XM_018093215.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017948704.1 Xenopus tropicalis calcium channel, voltage-dependent, T type, alpha 1I subunit (cacna1i), mRNA.

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CloneID	OXa43932
Clone ID Related Accession (Same CDS sequence)	XM_018093215.1
Accession Version	XM_018093215.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5892bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	voltage-dependent T-type calcium channel subunit alpha-1I
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 6% of CDS bases ##RefSeq-Attributes-END##

ATGCGGATCC TGGTGAATTT GCTGCTGGAC ACATTGCCTA TGCTGGGGAA TGTACTTCTG 
CTTTGCTTTT TTGTCTTCTT CATTTTTGGG ATCATTGGGG TGCAGCTTTG GGCCGGATTG 
CTGCGAAACC GCTGCTTTTT GGAAGAAAAC TTCACTATTC AAGGGGATAT AACCTTACCA 
CCTTACTACC AGCCAGAAGA AGATGATGAA ATGCCATTTA TTTGTTCTTT GTCTGGTGAC 
AATGGAATCA TGGGGTGCCA TGAAATCCCC CCATTAAGAG ACCGCGGTCA TGAGTGCTGT 
TTGGACAAGG AGGATTATTA TTATTATAAC TCTATCCGTC ACGAATTTAA TGTCAGCGGC 
ATGTGTGTTA ATTGGAACCA ATACTATAAC GTCTGCCGCA CCAGCAACAC CAATCCTCAC 
AAGGGCGCCA TCAATTTTGA TAACATTGGC TATGCCTGGA TTGTCATATT CCAGGTTATC 
ACTCTGGAAG GCTGGGTAGA GATAATGTAC TATGTCATGG ATGCCCACTC CTTTTACAAC 
TTCATCTATT TCATTCTTCT TATTATAGTG GGATCATTTT TCATGATCAA TTTATGCCTG 
GTTGTTATTG CTACACAATT CTCGGAGACC AAGCAGAGGG AGCACCAGCT GATGCAGGAG 
CAGAGGGCTC TCTATTTATC CTCTAGTACT GTAGCCAGTT ATGCTGAGCC TGGCGACTGC 
TACGAGGAGA TCTTTCAATA TGTGTGCCAT ATCCTGCGCA AGGCCAAGCG CCGCACAGTA 
GGCCTCTACA CTAGCCTTCA AAACCGACGG CAAAGTCGCC ATGAGCTGAC CAATGCTAAG 
CTTGGGATCA ATGGCAAGGA ACAGCATCAT CTGTGGATAT GTCAGAAACA TGGCCAGCTG 
GACTATGCTC CTCATGCTAT TGTGCAGCCT ATCGCAATGA CCCTAACGTC TGACCTCTCC 
AATTGCCCAC AATGCACCAA AACTAAATGG GAGGATGGCC GTAAGCTATC AGCACCAAAC 
AGCACCAGAT CAAGCCGAGA GGGGACTCAG AGACAGAGAA GTGAGAGCGA AAGGGAGAGC 
AGGAGTGATG GAAGCACATC TGATCTAGAC AAAGAGGAGG AGGAGGACGG TGATGAAGGC 
TTCTGTAAAG GACTCTGTGG CGAAGTCCGT GTAAAGCTCA GAGGAATCGT AGATAGTAAA 
TATTTTAATC GGGGAATTAT GATTGCCATT CTTGTGAACA CAATCAGTAT GGGAATAGAA 
CATCATGAGC AGCCAGAAGA ACTAACAAAT ATCCTGGAAA TCAGTAACAT TGTCTTTACC 
AGCATGTTTG CTCTGGAAAT GATGCTGAAG CTCTCAGCCT TTGGCCTCTT TGATTACCTG 
CGAAACCCAT ATAACATCTT TGATGGCATC ATTGTCATAA TCAGTGTCTG GGAGATAATT 
GGGCAGAATG ATGGAGGGCT GTCTGTACTG AGAACTTTCC GACTTCTGCG AGTACTGAAG 
CTGGTCCGGT TTATGCCTGC CTTAAGGCGT CAGTTGGTGG TTCTGATGAA GACTATGGAC 
AACGTGGCCA CATTCTGCAT GCTTCTGATG CTCTTCATCT TCATTTTCAG TATCCTTGGG 
ATGCATATCT TTGGATGTAA ATTCAGTTTA AGAACTGATT CTGGAGACAC TGTTCCAGAT 
AGGAAAAACT TTGATTCGCT ACTGTGGGCC ATTGTCACGG TTTTCCAGAT CCTTACCCAA 
GAGGACTGGA ATGTGGTGCT CTACAATGGA ATGGCCAGTA CATCTCCCTG GGCTGCGCTT 
TACTTTGTAG CATTAATGAC GTTTGGGAAT TACGTTTTAT TCAACTTGCT TGTCGCTATA 
TTGGTGGAAG GGTTTCAAGC AGAGGGAGAT GCCAACAGAT CTTATTCTGA TGAGGAAAGG 
AGTTCCTCCA ATATGGAGGA ATGTGATAAA TATCGGGAAG CTGAATCTCC ATCTGATTCC 
AAGCTTTGCT CAGTACCTAT GACTCCAAAT GGACATTTAG AAAACAGCCC TATTCCGACC 
CAACAAATCC CCACTACCAT AATGAATACA TCAAGGAATT CTCTTCAAAG GGACCAAGTT 
CTTGTAACTG TTGGCTGCAG AAAGAGTAGC GTGATGTCCC TGGGGAGAAC GTCCTACGAC 
CAGAGGTCAC AGATGGGGCC TCGACCCTCA CACTGGGGAA GAAGCAGTGC ATGGGGGAGC 
CGCAGATCAA GCTGGAACAG TTTAGGGCGT CGTCACAGTA TAAAGCCAAG AACATTCACA 
ACTGAACATG AATCATTGCT GTCACGTGGG GAGGAACAGA GGGAAAGAAG TCACTCCAGG 
AGATCCTCCA GCCTAAAGCA CAGAAGAGCA ATGTCTCTAG ATAATAAAGG CTCTTCAGAT 
AACCCAGGGG ATACAGTAAC TCATCCCAAC CATCATAGAG GCTCTTGCAA AGGAGCATCT 
ATGCCAGAAG AACATCGTGA ATCCATTGGG AAAACTCCAC CAATCATGAA GGAAGTGTTT 
CCCAAAATGA ATAACCGCAA AGATAAGGAA GAGGAAGAAG AAGAAATAGA TTATAGTCTG 
TGCTTCCGGA TCCGGAAAAT GATTGAAGTG TACAGACCAG ACTGGTGCGA GTTACGTGAG 
GACTGGTCTA TATTTCTATT CTCCCCACAG AACAGGTTCC GCATCATGTG CCAAACAATA 
ATTGCGCACA AGCTTTTTGA CTATGTGGTC CTTGCCTTCA TCTTCCTCAA CTGCATTACT 
ATCGCCTTGG AGAGGCCTCA GATAGAGCAC AAGAGTACGG AAAGGCTGTT CCTGAGTATA 
TCAAATTATA TCTTCACAGC CATTTTTGTA GCTGAAATGA CATTGAAGGT GGTCTCTCTG 
GGACTTTACT TTGGGGATCA AGCTTACCTC CGGAGCAGCT GGAATATTCT GGATGGGTTT 
CTCGTCTTTG TATCCCTCAT TGATATTGTG GTGTCTGTAG CTTCAGCCGG TGGAGCAAAA 
ATTCTGGGTG TCTTAAGGGT TCTTCGGCTT TTGAGGACAT TGCGACCTCT CAGGGTAATC 
AGTAGAGCCC CTGGCCTGAA GCTGGTGGTG GAGACCCTGA TTTCTTCTCT AAAACCCATT 
GGAAACATAG TTCTGATCTG CTGTGCCTTC TTTATTATTT TTGGAATCCT TGGTGTGCAG 
CTTTTTAAGG GTAAATTTTT CTACTGCCTT GGGGTGGACG TTCGAAATGT CACCAATAAG 
TCAGACTGTG AGGCGGCCAA TTATAAATGG GTGCATCATA AGTACAATTT CGATAACCTG 
GGGCAGGCAT TAATGTCGCT TTTTGTCCTG GCATCAAAGG ACGGATGGGT TAATATAATG 
TATAATGGAC TTGATGCCGT GGCAGTGGAC CAACAGCCAG TGACCAATCA CAACCCATGG 
ATGTTGCTGT ACTTTATCTC ATTTCTGCTG ATCGTCAGTT TCTTTGTACT GAACATGTTT 
GTGGGTGTCG TGGTTGAGAA CTTTCACAAA TGTCGGCAGC ATCAGGAAGC CGAGGAGGCC 
CGAAGACGAG AAGAAAAACG CTTGAGGCGC CTGGAGAAAA AACGAAGGAA AGCCCAGCGC 
TTACCGTACT ATGCCACCTA TTGTCCTGCT CGCCTCCTTA TTCATTCCGT CTGCACCAGC 
CACTACTTGG ATATTTTCAT TACAATCATC ATTTGCCTTA ACGTTATCAC AATGTCCTTG 
GAGCACTATA GTCAACCAAG GTCTCTTGAA ATTGCTCTAA AGTACTGTAA CTACATGTTT 
ACTACAGTCT TTGTACTTGA AGCTGTGCTG AAGCTGGTGG CCTTTGGATT CAGACGTTTC 
TTCAAGGACA GGTGGAACCA ACTGGACCTG GCCATTGTGC TCCTGTCTGT GATGGGAATC 
ACTTTAGAAG AAATTGAAAT AAATGCTGCT TTGCCCATCA ATCCCACCAT TATTCGCATC 
ATGAGGGTGC TGCGCATCAC CAGGGTGCTG AAGCTGCTTA AAATGGCAAC AGGCATGAGG 
GCGCTACTGG ACACTGTGGT ACAAGCTTTG CCTCAGGTAG GAAACTTGGG ACTGCTCTTC 
ATGCTTCTCT TCTTCATCTA TGCTGCTCTG GGAGTGGAGC TCTTCGGGAA GCTTGTTTGC 
AATGATGATC ACCCGTGTGA AGGAATGAGT CGCCACGCCA CATTTGAGAA TTTTGGAATG 
GCCTTCCTGA CCCTCTTTCA GGTTTCTACT GGGGACAACT GGAATGGAAT AATGAAGGAT 
ACCTTGCGTG ACTGCACCAG TGATGATCGC AGTTGCTTGA GCAACCTCCA GTTCATCTCG 
CCTTTGTACT TTGTGAGCTT TGTGCTCACA GCTCAGTTTG TGCTAATTAA CGTAGTAGTC 
GCTGTGTTAA TGAAACACCT GGATGACAGC AATAAAGAAG CGCAGGAGGA CGCTGAGCTG 
GATGCGGAGA TTGAAATGGA GTTGGCTCAT GGCCTCTGTT CTCAAACATC GCCGGTCTCT 
GGTTTAGATA ATGGAGGACG CGGAGGAAGA CCTGCAACTG ACAGTCATCG TAGCTTAAGG 
TGTTTTTCTC CTGCTCAGGA GAATTTGTGG TTGGACAGTG TGTCCTTAAT CATTAAGGAT 
TCCTTTGAAG GAGAGCTAAC AATCATTGAT AATTTATCTG GATCAGTCTT CCACCATTAC 
TCCTCCCCAG CCGTCTGTGA GAAGTGCAAC CACGACAAAC AAGAGGTCCA GCTGGCAGAA 
ATGGAGGCCT TTTCTTTAAC CTCTGACAAG TCCTCGTCAG TACTGACTGA TGAACTCAGT 
TCAGAAGATC AAGGTGTCAC GAGTCAGTTA AAGGAGATGA AGGATGGGCA AGATAGTCCT 
GATTCCTTGG GAATGTTTGG AGAAAATATC TTCCCACTTG CAAGCATTCC CGGTTCCCCA 
ACAGCGGAGC ACTTTATGTG TGAGTTTGAG AAGGTCTCAT TAACCCCGGC TGAGTCATGG 
GAAAGCTGGC TGAAGGAAGA GAGCAGCCCA GTCTCTCCTT CCCCGCTATG TGCGGATACA 
ATGAGCCCTC TTTTACCAGT CCCAGCAGAA TTTTTCCATC CTGATCTCTC TGCAACTGAA 
ACAGATCGAG AGATTCCAAA CACCAGAGAA AATACTTCCA AACTGCCATC TCAAGAAACG 
TGGGCAACTC TGCACTCTCC AGATATCAAT TGCTCACTTC TTCGGCAGGC AACAGAAAGC 
AATTCATCTA TGGAAAGTGG TAGTGCAACG TCTGAGGACA GTTTACAGAC CACATTAGAA 
GATAGCCTAA ATCTTAGTGA CTCACCCCAG TGCACATTGG ATCTCCCAGA TCTCCGAGCA 
ACACCTGCAT CGCACCATAC TCCATTGCCT ATATCATCTA TATCCATGAT GCAAAGGAGA 
AGTAACCGCC CTAGCTTTGC CCTTCAGAAA GTTCGACGTC ACCAACGGAG CCATAGCAGT 
GGTGGTAGCA CTAGTCCTGG ATGTACTCTT CATGACTCCA TGGACCCTTC TGATGAGGAA 
GGGGCAGGTG GAAGCCTGCG AGGTGTGAGC GAGCCCTCTG AGACATTTAG CAGCTTATCT 
CTGACCTCCC TCTTTTCACC ACCTCCTTCT TTAGCACCAC CCGGGGGCAC AATTAAAAAA 
TGCAGCAGTG TCAATAGTCT TTCTGTTGCT CCTTCTAGGG TAAGAGGAGC AAAGCAATTT 
TACACAGTTG ATCCACAGGG CTTCCTTTCT ATGCCATCTT GGGTTATGGA TTTTTACCAA 
ACTTCGACTC CCTCAGAAAA CCAAGAGAAT AGTAGCAGTA CCGAAAAATT ACCAACTATG 
GAAAAGGGGT TACCACCCAG TAGTGTCTCA GGAAATGTGG ATTTTGGCAA TAACAGTCGT 
CGAAACAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017948704.1
CDS	481..6372
Translation	MRILVNLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWAGLLRNRCFLEENFTIQGDITLPPYYQPEEDDEMPFICSLSGDNGIMGCHEIPPLRDRGHECCLDKEDYYYYNSIRHEFNVSGMCVNWNQYYNVCRTSNTNPHKGAINFDNIGYAWIVIFQVITLEGWVEIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQREHQLMQEQRALYLSSSTVASYAEPGDCYEEIFQYVCHILRKAKRRTVGLYTSLQNRRQSRHELTNAKLGINGKEQHHLWICQKHGQLDYAPHAIVQPIAMTLTSDLSNCPQCTKTKWEDGRKLSAPNSTRSSREGTQRQRSESERESRSDGSTSDLDKEEEEDGDEGFCKGLCGEVRVKLRGIVDSKYFNRGIMIAILVNTISMGIEHHEQPEELTNILEISNIVFTSMFALEMMLKLSAFGLFDYLRNPYNIFDGIIVIISVWEIIGQNDGGLSVLRTFRLLRVLKLVRFMPALRRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHIFGCKFSLRTDSGDTVPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMASTSPWAALYFVALMTFGNYVLFNLLVAILVEGFQAEGDANRSYSDEERSSSNMEECDKYREAESPSDSKLCSVPMTPNGHLENSPIPTQQIPTTIMNTSRNSLQRDQVLVTVGCRKSSVMSLGRTSYDQRSQMGPRPSHWGRSSAWGSRRSSWNSLGRRHSIKPRTFTTEHESLLSRGEEQRERSHSRRSSSLKHRRAMSLDNKGSSDNPGDTVTHPNHHRGSCKGASMPEEHRESIGKTPPIMKEVFPKMNNRKDKEEEEEEIDYSLCFRIRKMIEVYRPDWCELREDWSIFLFSPQNRFRIMCQTIIAHKLFDYVVLAFIFLNCITIALERPQIEHKSTERLFLSISNYIFTAIFVAEMTLKVVSLGLYFGDQAYLRSSWNILDGFLVFVSLIDIVVSVASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLKPIGNIVLICCAFFIIFGILGVQLFKGKFFYCLGVDVRNVTNKSDCEAANYKWVHHKYNFDNLGQALMSLFVLASKDGWVNIMYNGLDAVAVDQQPVTNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLEKKRRKAQRLPYYATYCPARLLIHSVCTSHYLDIFITIIICLNVITMSLEHYSQPRSLEIALKYCNYMFTTVFVLEAVLKLVAFGFRRFFKDRWNQLDLAIVLLSVMGITLEEIEINAALPINPTIIRIMRVLRITRVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGKLVCNDDHPCEGMSRHATFENFGMAFLTLFQVSTGDNWNGIMKDTLRDCTSDDRSCLSNLQFISPLYFVSFVLTAQFVLINVVVAVLMKHLDDSNKEAQEDAELDAEIEMELAHGLCSQTSPVSGLDNGGRGGRPATDSHRSLRCFSPAQENLWLDSVSLIIKDSFEGELTIIDNLSGSVFHHYSSPAVCEKCNHDKQEVQLAEMEAFSLTSDKSSSVLTDELSSEDQGVTSQLKEMKDGQDSPDSLGMFGENIFPLASIPGSPTAEHFMCEFEKVSLTPAESWESWLKEESSPVSPSPLCADTMSPLLPVPAEFFHPDLSATETDREIPNTRENTSKLPSQETWATLHSPDINCSLLRQATESNSSMESGSATSEDSLQTTLEDSLNLSDSPQCTLDLPDLRATPASHHTPLPISSISMMQRRSNRPSFALQKVRRHQRSHSSGGSTSPGCTLHDSMDPSDEEGAGGSLRGVSEPSETFSSLSLTSLFSPPPSLAPPGGTIKKCSSVNSLSVAPSRVRGAKQFYTVDPQGFLSMPSWVMDFYQTSTPSENQENSSSTEKLPTMEKGLPPSSVSGNVDFGNNSRRNR

Target ORF information:

RefSeq Version	XM_018093215.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calcium channel, voltage-dependent, T type, alpha 1I subunit (cacna1i), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018093215.1

ORF Insert Sequence:

ATGCGGATCC TGGTGAATTT GCTGCTGGAC ACATTGCCTA TGCTGGGGAA TGTACTTCTG 
CTTTGCTTTT TTGTCTTCTT CATTTTTGGG ATCATTGGGG TGCAGCTTTG GGCCGGATTG 
CTGCGAAACC GCTGCTTTTT GGAAGAAAAC TTCACTATTC AAGGGGATAT AACCTTACCA 
CCTTACTACC AGCCAGAAGA AGATGATGAA ATGCCATTTA TTTGTTCTTT GTCTGGTGAC 
AATGGAATCA TGGGGTGCCA TGAAATCCCC CCATTAAGAG ACCGCGGTCA TGAGTGCTGT 
TTGGACAAGG AGGATTATTA TTATTATAAC TCTATCCGTC ACGAATTTAA TGTCAGCGGC 
ATGTGTGTTA ATTGGAACCA ATACTATAAC GTCTGCCGCA CCAGCAACAC CAATCCTCAC 
AAGGGCGCCA TCAATTTTGA TAACATTGGC TATGCCTGGA TTGTCATATT CCAGGTTATC 
ACTCTGGAAG GCTGGGTAGA GATAATGTAC TATGTCATGG ATGCCCACTC CTTTTACAAC 
TTCATCTATT TCATTCTTCT TATTATAGTG GGATCATTTT TCATGATCAA TTTATGCCTG 
GTTGTTATTG CTACACAATT CTCGGAGACC AAGCAGAGGG AGCACCAGCT GATGCAGGAG 
CAGAGGGCTC TCTATTTATC CTCTAGTACT GTAGCCAGTT ATGCTGAGCC TGGCGACTGC 
TACGAGGAGA TCTTTCAATA TGTGTGCCAT ATCCTGCGCA AGGCCAAGCG CCGCACAGTA 
GGCCTCTACA CTAGCCTTCA AAACCGACGG CAAAGTCGCC ATGAGCTGAC CAATGCTAAG 
CTTGGGATCA ATGGCAAGGA ACAGCATCAT CTGTGGATAT GTCAGAAACA TGGCCAGCTG 
GACTATGCTC CTCATGCTAT TGTGCAGCCT ATCGCAATGA CCCTAACGTC TGACCTCTCC 
AATTGCCCAC AATGCACCAA AACTAAATGG GAGGATGGCC GTAAGCTATC AGCACCAAAC 
AGCACCAGAT CAAGCCGAGA GGGGACTCAG AGACAGAGAA GTGAGAGCGA AAGGGAGAGC 
AGGAGTGATG GAAGCACATC TGATCTAGAC AAAGAGGAGG AGGAGGACGG TGATGAAGGC 
TTCTGTAAAG GACTCTGTGG CGAAGTCCGT GTAAAGCTCA GAGGAATCGT AGATAGTAAA 
TATTTTAATC GGGGAATTAT GATTGCCATT CTTGTGAACA CAATCAGTAT GGGAATAGAA 
CATCATGAGC AGCCAGAAGA ACTAACAAAT ATCCTGGAAA TCAGTAACAT TGTCTTTACC 
AGCATGTTTG CTCTGGAAAT GATGCTGAAG CTCTCAGCCT TTGGCCTCTT TGATTACCTG 
CGAAACCCAT ATAACATCTT TGATGGCATC ATTGTCATAA TCAGTGTCTG GGAGATAATT 
GGGCAGAATG ATGGAGGGCT GTCTGTACTG AGAACTTTCC GACTTCTGCG AGTACTGAAG 
CTGGTCCGGT TTATGCCTGC CTTAAGGCGT CAGTTGGTGG TTCTGATGAA GACTATGGAC 
AACGTGGCCA CATTCTGCAT GCTTCTGATG CTCTTCATCT TCATTTTCAG TATCCTTGGG 
ATGCATATCT TTGGATGTAA ATTCAGTTTA AGAACTGATT CTGGAGACAC TGTTCCAGAT 
AGGAAAAACT TTGATTCGCT ACTGTGGGCC ATTGTCACGG TTTTCCAGAT CCTTACCCAA 
GAGGACTGGA ATGTGGTGCT CTACAATGGA ATGGCCAGTA CATCTCCCTG GGCTGCGCTT 
TACTTTGTAG CATTAATGAC GTTTGGGAAT TACGTTTTAT TCAACTTGCT TGTCGCTATA 
TTGGTGGAAG GGTTTCAAGC AGAGGGAGAT GCCAACAGAT CTTATTCTGA TGAGGAAAGG 
AGTTCCTCCA ATATGGAGGA ATGTGATAAA TATCGGGAAG CTGAATCTCC ATCTGATTCC 
AAGCTTTGCT CAGTACCTAT GACTCCAAAT GGACATTTAG AAAACAGCCC TATTCCGACC 
CAACAAATCC CCACTACCAT AATGAATACA TCAAGGAATT CTCTTCAAAG GGACCAAGTT 
CTTGTAACTG TTGGCTGCAG AAAGAGTAGC GTGATGTCCC TGGGGAGAAC GTCCTACGAC 
CAGAGGTCAC AGATGGGGCC TCGACCCTCA CACTGGGGAA GAAGCAGTGC ATGGGGGAGC 
CGCAGATCAA GCTGGAACAG TTTAGGGCGT CGTCACAGTA TAAAGCCAAG AACATTCACA 
ACTGAACATG AATCATTGCT GTCACGTGGG GAGGAACAGA GGGAAAGAAG TCACTCCAGG 
AGATCCTCCA GCCTAAAGCA CAGAAGAGCA ATGTCTCTAG ATAATAAAGG CTCTTCAGAT 
AACCCAGGGG ATACAGTAAC TCATCCCAAC CATCATAGAG GCTCTTGCAA AGGAGCATCT 
ATGCCAGAAG AACATCGTGA ATCCATTGGG AAAACTCCAC CAATCATGAA GGAAGTGTTT 
CCCAAAATGA ATAACCGCAA AGATAAGGAA GAGGAAGAAG AAGAAATAGA TTATAGTCTG 
TGCTTCCGGA TCCGGAAAAT GATTGAAGTG TACAGACCAG ACTGGTGCGA GTTACGTGAG 
GACTGGTCTA TATTTCTATT CTCCCCACAG AACAGGTTCC GCATCATGTG CCAAACAATA 
ATTGCGCACA AGCTTTTTGA CTATGTGGTC CTTGCCTTCA TCTTCCTCAA CTGCATTACT 
ATCGCCTTGG AGAGGCCTCA GATAGAGCAC AAGAGTACGG AAAGGCTGTT CCTGAGTATA 
TCAAATTATA TCTTCACAGC CATTTTTGTA GCTGAAATGA CATTGAAGGT GGTCTCTCTG 
GGACTTTACT TTGGGGATCA AGCTTACCTC CGGAGCAGCT GGAATATTCT GGATGGGTTT 
CTCGTCTTTG TATCCCTCAT TGATATTGTG GTGTCTGTAG CTTCAGCCGG TGGAGCAAAA 
ATTCTGGGTG TCTTAAGGGT TCTTCGGCTT TTGAGGACAT TGCGACCTCT CAGGGTAATC 
AGTAGAGCCC CTGGCCTGAA GCTGGTGGTG GAGACCCTGA TTTCTTCTCT AAAACCCATT 
GGAAACATAG TTCTGATCTG CTGTGCCTTC TTTATTATTT TTGGAATCCT TGGTGTGCAG 
CTTTTTAAGG GTAAATTTTT CTACTGCCTT GGGGTGGACG TTCGAAATGT CACCAATAAG 
TCAGACTGTG AGGCGGCCAA TTATAAATGG GTGCATCATA AGTACAATTT CGATAACCTG 
GGGCAGGCAT TAATGTCGCT TTTTGTCCTG GCATCAAAGG ACGGATGGGT TAATATAATG 
TATAATGGAC TTGATGCCGT GGCAGTGGAC CAACAGCCAG TGACCAATCA CAACCCATGG 
ATGTTGCTGT ACTTTATCTC ATTTCTGCTG ATCGTCAGTT TCTTTGTACT GAACATGTTT 
GTGGGTGTCG TGGTTGAGAA CTTTCACAAA TGTCGGCAGC ATCAGGAAGC CGAGGAGGCC 
CGAAGACGAG AAGAAAAACG CTTGAGGCGC CTGGAGAAAA AACGAAGGAA AGCCCAGCGC 
TTACCGTACT ATGCCACCTA TTGTCCTGCT CGCCTCCTTA TTCATTCCGT CTGCACCAGC 
CACTACTTGG ATATTTTCAT TACAATCATC ATTTGCCTTA ACGTTATCAC AATGTCCTTG 
GAGCACTATA GTCAACCAAG GTCTCTTGAA ATTGCTCTAA AGTACTGTAA CTACATGTTT 
ACTACAGTCT TTGTACTTGA AGCTGTGCTG AAGCTGGTGG CCTTTGGATT CAGACGTTTC 
TTCAAGGACA GGTGGAACCA ACTGGACCTG GCCATTGTGC TCCTGTCTGT GATGGGAATC 
ACTTTAGAAG AAATTGAAAT AAATGCTGCT TTGCCCATCA ATCCCACCAT TATTCGCATC 
ATGAGGGTGC TGCGCATCAC CAGGGTGCTG AAGCTGCTTA AAATGGCAAC AGGCATGAGG 
GCGCTACTGG ACACTGTGGT ACAAGCTTTG CCTCAGGTAG GAAACTTGGG ACTGCTCTTC 
ATGCTTCTCT TCTTCATCTA TGCTGCTCTG GGAGTGGAGC TCTTCGGGAA GCTTGTTTGC 
AATGATGATC ACCCGTGTGA AGGAATGAGT CGCCACGCCA CATTTGAGAA TTTTGGAATG 
GCCTTCCTGA CCCTCTTTCA GGTTTCTACT GGGGACAACT GGAATGGAAT AATGAAGGAT 
ACCTTGCGTG ACTGCACCAG TGATGATCGC AGTTGCTTGA GCAACCTCCA GTTCATCTCG 
CCTTTGTACT TTGTGAGCTT TGTGCTCACA GCTCAGTTTG TGCTAATTAA CGTAGTAGTC 
GCTGTGTTAA TGAAACACCT GGATGACAGC AATAAAGAAG CGCAGGAGGA CGCTGAGCTG 
GATGCGGAGA TTGAAATGGA GTTGGCTCAT GGCCTCTGTT CTCAAACATC GCCGGTCTCT 
GGTTTAGATA ATGGAGGACG CGGAGGAAGA CCTGCAACTG ACAGTCATCG TAGCTTAAGG 
TGTTTTTCTC CTGCTCAGGA GAATTTGTGG TTGGACAGTG TGTCCTTAAT CATTAAGGAT 
TCCTTTGAAG GAGAGCTAAC AATCATTGAT AATTTATCTG GATCAGTCTT CCACCATTAC 
TCCTCCCCAG CCGTCTGTGA GAAGTGCAAC CACGACAAAC AAGAGGTCCA GCTGGCAGAA 
ATGGAGGCCT TTTCTTTAAC CTCTGACAAG TCCTCGTCAG TACTGACTGA TGAACTCAGT 
TCAGAAGATC AAGGTGTCAC GAGTCAGTTA AAGGAGATGA AGGATGGGCA AGATAGTCCT 
GATTCCTTGG GAATGTTTGG AGAAAATATC TTCCCACTTG CAAGCATTCC CGGTTCCCCA 
ACAGCGGAGC ACTTTATGTG TGAGTTTGAG AAGGTCTCAT TAACCCCGGC TGAGTCATGG 
GAAAGCTGGC TGAAGGAAGA GAGCAGCCCA GTCTCTCCTT CCCCGCTATG TGCGGATACA 
ATGAGCCCTC TTTTACCAGT CCCAGCAGAA TTTTTCCATC CTGATCTCTC TGCAACTGAA 
ACAGATCGAG AGATTCCAAA CACCAGAGAA AATACTTCCA AACTGCCATC TCAAGAAACG 
TGGGCAACTC TGCACTCTCC AGATATCAAT TGCTCACTTC TTCGGCAGGC AACAGAAAGC 
AATTCATCTA TGGAAAGTGG TAGTGCAACG TCTGAGGACA GTTTACAGAC CACATTAGAA 
GATAGCCTAA ATCTTAGTGA CTCACCCCAG TGCACATTGG ATCTCCCAGA TCTCCGAGCA 
ACACCTGCAT CGCACCATAC TCCATTGCCT ATATCATCTA TATCCATGAT GCAAAGGAGA 
AGTAACCGCC CTAGCTTTGC CCTTCAGAAA GTTCGACGTC ACCAACGGAG CCATAGCAGT 
GGTGGTAGCA CTAGTCCTGG ATGTACTCTT CATGACTCCA TGGACCCTTC TGATGAGGAA 
GGGGCAGGTG GAAGCCTGCG AGGTGTGAGC GAGCCCTCTG AGACATTTAG CAGCTTATCT 
CTGACCTCCC TCTTTTCACC ACCTCCTTCT TTAGCACCAC CCGGGGGCAC AATTAAAAAA 
TGCAGCAGTG TCAATAGTCT TTCTGTTGCT CCTTCTAGGG TAAGAGGAGC AAAGCAATTT 
TACACAGTTG ATCCACAGGG CTTCCTTTCT ATGCCATCTT GGGTTATGGA TTTTTACCAA 
ACTTCGACTC CCTCAGAAAA CCAAGAGAAT AGTAGCAGTA CCGAAAAATT ACCAACTATG 
GAAAAGGGGT TACCACCCAG TAGTGTCTCA GGAAATGTGG ATTTTGGCAA TAACAGTCGT 
CGAAACAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1i ( XM_018093215.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017948704.2 Xenopus tropicalis calcium channel, voltage-dependent, T type, alpha 1I subunit (cacna1i), mRNA.

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CloneID	OXa57430
Clone ID Related Accession (Same CDS sequence)	XM_018093215.2
Accession Version	XM_018093215.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	voltage-dependent T-type calcium channel subunit alpha-1I
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018093215.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGTAA TTCTTCTGAA CTGTGTAACC CTCGGAATGT TCCAGCCTTG TGAAGATGTC 
GACTGCTCAT CAGAGCGATG CCAAATTCTT CAAGTCTTCG ATGACTTTAT ATTCATCTTC 
TTTGCAATGG AGATGGTCGT GAAAATGGTG GCCTTGGGCA TCTTTGGGAA GAAGTGTTAC 
CTTGGAGATA CATGGAATAG GTTGGACTTC TTTATAGTCA TGGCTGGGAT GGTGGAATAT 
TCTCTTGATC TTCAGAACAT TAATCTATCG GCCATCCGCA CTGTGCGTGT TCTGAGACCC 
CTAAAAGCCA TCAACCGAGT ACCCAGCATG CGGATCCTGG TGAATTTGCT GCTGGACACA 
TTGCCTATGC TGGGGAATGT ACTTCTGCTT TGCTTTTTTG TCTTCTTCAT TTTTGGGATC 
ATTGGGGTGC AGCTTTGGGC CGGATTGCTG CGAAACCGCT GCTTTTTGGA AGAAAACTTC 
ACTATTCAAG GGGATATAAC CTTACCACCT TACTACCAGC CAGAAGAAGA TGATGAAATG 
CCATTTATTT GTTCTTTGTC TGGTGACAAT GGAATCATGG GGTGCCATGA AATCCCCCCA 
TTAAGAGACC GCGGTCATGA GTGCTGTTTG GACAAGGAGG ATTATTATTA TTATAACTCT 
ATCCGTCACG AATTTAATGT CAGCGGCATG TGTGTTAATT GGAACCAATA CTATAACGTC 
TGCCGCACCA GCAACACCAA TCCTCACAAG GGCGCCATCA ATTTTGATAA CATTGGCTAT 
GCCTGGATTG TCATATTCCA GGTTATCACT CTGGAAGGCT GGGTAGAGAT AATGTACTAT 
GTCATGGATG CCCACTCCTT TTACAACTTC ATCTATTTCA TTCTTCTTAT TATAGTGGGA 
TCATTTTTCA TGATCAATTT ATGCCTGGTT GTTATTGCTA CACAATTCTC GGAGACCAAG 
CAGAGGGAGC ACCAGCTGAT GCAGGAGCAG AGGGCTCTCT ATTTATCCTC TAGTACTGTA 
GCCAGTTATG CTGAGCCTGG CGACTGCTAC GAGGAGATCT TTCAATATGT GTGCCATATC 
CTGCGCAAGG CCAAGCGCCG TACAGTAGGC CTCTACACTA GCCTTCAAAA CCGACGGCAA 
AGTCGCCATG AGCTGACCAA TGCTAAGCTT GGGATCAATG GCAAGGAACA GCATCATCTG 
TGGATATGTC AGAAACATGG CCAGCTGGAC TATGCTCCTC ATGCTATTGT GCAGCCTATC 
GCAATGACCT TAACGTCTGA CCTCTCCAAT TGCCCACAAT GCACCAAAAC CAAATGGGAG 
GATGGCCGTA AGCTATCAGC ACCAAACAGC ACCAGATCAA GCCGAGAGGG GACTCAGAGA 
CAGAGAAGTG AGAGCGAAAG GGAGAGCAGG AGTGATGGAA GCACATCCGA TCTAGACAAA 
GAAGAGGAGG AGGACGGTGA TGAAGGCTTC TGTAAAGGAC TCTGTGGCGA AGTCCGTGTA 
AAGCTCAGAG GAATCGTAGA TAGTAAATAT TTTAATCGGG GAATTATGAT TGCCATTCTT 
GTGAACACAA TCAGTATGGG AATAGAACAT CATGAGCAGC CAGAAGAACT AACAAATATC 
CTGGAAATCA GTAACATTGT CTTTACCAGC ATGTTTGCTC TGGAAATGAT GCTGAAGCTC 
TCAGCCTTTG GCCTCTTTGA TTACCTGCGA AACCCATATA ACATCTTTGA TGGCATCATT 
GTCATAATCA GTGTCTGGGA GATAATTGGG CAGAATGATG GAGGGCTGTC TGTACTGAGA 
ACTTTCCGAC TTCTGCGAGT ACTGAAGCTG GTCCGGTTTA TGCCTGCCTT AAGGCGTCAG 
TTGGTGGTTC TGATGAAGAC TATGGACAAC GTGGCCACAT TCTGCATGCT TCTGATGCTC 
TTCATCTTCA TTTTCAGTAT CCTTGGGATG CATATCTTTG GATGTAAATT CAGTTTAAGA 
ACTGATTCTG GAGACACTGT TCCAGATAGG AAAAACTTTG ATTCGCTACT GTGGGCCATT 
GTCACGGTTT TCCAGATCCT TACCCAAGAG GACTGGAATG TGGTGCTCTA CAATGGAATG 
GCCAGTACAT CTCCCTGGGC TGCGCTTTAC TTTGTAGCAT TAATGACGTT TGGGAATTAC 
GTTTTATTCA ACTTGCTTGT CGCTATATTG GTGGAAGGGT TTCAAGCAGA GGGAGATGCC 
AACAGATCTT ATTCTGATGA GGAAAGGAGT TCCTCCAATA TGGAGGAATG TGATAAATAT 
CGGGAAGCTG AATCTCCATC TGATTCCAAG CTTTGCTCAG TACCTATGAC TCCAAATGGA 
CATTTAGAAA ACAGCCCTAT TCCGACCCAA CAAATCCCCA CTACCATAAT GAATACATCA 
AGGAATTCTC TTCAAAGGGA CCAAGTTCTT GTAACTGTTG GCTGCAGAAA GAGTAGCGTG 
ATGTCCCTGG GGAGAACGTC CTACGACCAG AGGTCACAGA TGGGGCCTCG ACCCTCACAC 
TGGGGAAGAA GCAGTGCATG GGGGAGCCGC AGATCAAGCT GGAACAGTTT AGGGCGTCGT 
CACAGTATAA AGCCAAGAAC ATTCACAACT GAACATGAAT CATTGCTGTC ACGTGGGGAG 
GAACAGAGGG AAAGAAGTCA CTCCAGGAGA TCCTCCAGCC TAAAGCACAG AAGAGCAATG 
TCTCTAGATA ATAAAGGCTC TTCAGATAAC CCAGGGGATA CAGTAACTCA TCCCAACCAT 
CATAGAGGCT CTTGCAAAGG AGCATCTATG CCAGAAGAAC ATCGTGAATC CATTGGGAAA 
ACTCCACCAA TCATGAAGGA AGTGTTTCCC AAAATGAATA ACCGCAAAGA TAAGGAAGAG 
GAAGAAGAAG AAATAGATTA TAGTCTGTGC TTCCGGATCC GGAAAATGAT TGAAGTGTAC 
AGACCAGACT GGTGCGAGTT ACGTGAGGAC TGGTCTATAT TTCTATTCTC CCCACAGAAC 
AGGTTCCGCA TCATGTGCCA AACAATAATT GCGCACAAGC TTTTTGACTA TGTGGTCCTT 
GCCTTCATCT TCCTCAACTG CATTACTATC GCCTTGGAGA GGCCTCAGAT AGAGCACAAG 
AGTACGGAAA GGCTGTTCCT GAGTATATCA AATTATATCT TCACAGCCAT TTTTGTAGCT 
GAAATGACAT TGAAGGTGGT CTCTCTGGGA CTTTACTTTG GGGATCAAGC TTACCTCCGG 
AGCAGCTGGA ATATTCTGGA TGGGTTTCTC GTCTTTGTAT CCCTCATTGA TATTGTGGTG 
TCTGTAGCTT CAGCCGGTGG AGCAAAAATT CTGGGTGTCT TAAGGGTTCT TCGGCTTTTG 
AGGACATTGC GACCTCTCAG GGTTATCAGT AGAGCCCCTG GCCTGAAGCT GGTGGTGGAG 
ACCCTGATTT CTTCTCTAAA ACCCATTGGA AACATAGTTC TGATCTGCTG TGCCTTCTTT 
ATTATTTTTG GAATCCTTGG TGTGCAGCTT TTTAAGGGTA AATTTTTCTA CTGCCTTGGG 
GTGGACGTTC GAAATGTCAC CAATAAGTCA GACTGTGAGG CGGCCAATTA TAAATGGGTG 
CACCATAAGT ACAATTTCGA TAACCTGGGG CAGGCATTAA TGTCGCTTTT TGTCCTGGCA 
TCAAAGGACG GATGGGTTAA TATAATGTAT AATGGACTTG ATGCCGTGGC AGTGGACCAA 
CAGCCAGTGA CCAATCACAA CCCATGGATG TTGCTGTACT TTATCTCATT TCTGCTGATC 
GTCAGTTTCT TTGTACTGAA CATGTTTGTG GGTGTCGTGG TTGAGAACTT TCACAAATGT 
CGGCAGCATC AGGAAGCCGA GGAGGCCCGA AGACGAGAAG AAAAACGCTT GAGGCGCCTG 
GAGAAAAAAC GAAGGAAAGC CCAGCGCTTA CCGTACTATG CCACCTATTG TCCTGCTCGC 
CTCCTTATTC ATTCCGTCTG CACCAGCCAC TACTTGGATA TTTTCATTAC AATCATCATT 
TGCCTTAACG TTATCACAAT GTCCTTGGAG CACTATAGTC AACCAAGGTC TCTTGAAATT 
GCTCTAAAGT ACTGTAACTA CATGTTTACT ACAGTCTTTG TACTTGAAGC TGTGCTGAAG 
CTGGTGGCCT TTGGATTCAG ACGTTTCTTC AAGGACAGGT GGAACCAACT GGACCTGGCC 
ATTGTGCTCC TGTCTGTGAT GGGAATCACT TTAGAAGAAA TTGAAATAAA TGCTGCTTTG 
CCCATCAATC CCACCATTAT TCGCATCATG AGGGTGCTGC GCATCACCAG GGTGCTGAAG 
CTGCTTAAAA TGGCAACAGG CATGAGGGCG CTACTGGACA CTGTGGTACA AGCTTTGCCT 
CAGGTAGGAA ACTTGGGACT GCTCTTCATG CTTCTCTTCT TCATCTATGC TGCTCTGGGA 
GTGGAGCTCT TCGGGAAGCT TGTTTGCAAT GATGATCACC CGTGTGAAGG AATGAGTCGC 
CACGCCACAT TTGAGAATTT TGGAATGGCC TTCCTGACCC TCTTTCAGGT TTCTACTGGG 
GACAACTGGA ATGGAATAAT GAAGGATACC TTGCGTGACT GCACCAGTGA TGATCGCAGT 
TGCTTGAGCA ACCTCCAGTT CATCTCGCCT TTGTACTTTG TGAGCTTTGT GCTCACAGCT 
CAGTTTGTGC TAATTAACGT AGTAGTCGCT GTGTTAATGA AACACCTGGA TGACAGCAAT 
AAAGAAGCGC AGGAGGACGC TGAGCTGGAT GCGGAGATTG AAATGGAGTT GGCTCATGGC 
CTCTGTTCTC AAACATCGCC GGTCTCTGGT TTAGATAATG GAGGACGCGG AGGAAGACCT 
GCAACTGACA GTCATCGTAG CTTAAGGTGT TTTTCTCCTG CTCAGGAGAA TTTGTGGTTG 
GACAGTGTGT CCTTAATCAT TAAGGATTCC TTTGAAGGAG AGCTAACAAT CATTGATAAT 
TTATCTGGAT CAGTCTTCCA CCATTACTCC TCCCCAGCCG TCTGTGAGAA GTGCAACCAC 
GACAAACAAG AGGTCCAGCT GGCAGAAATG GAGGCCTTTT CTTTAACCTC TGACAAGTCC 
TCGTCAGTAC TGACTGATGA ACTCAGTTCA GAAGATCAAG GTGTCACGAG TCAGTTAAAG 
GAGATGAAGG ATGGGCAAGA TAGTCCTGAT TCCTTGGGAA TGTTTGGAGA AAATATCTTC 
CCACTTGCAA GCATTCCCGG TTCCCCAACA GCGGAGCACT TTATGTGTGA GTTTGAGAAG 
GTCTCATTAA CCCCGGCTGA GTCATGGGAA AGCTGGCTGA AGGAAGAGAG CAGCCCAGTC 
TCTCCTTCCC CGCTATGTGC GGATACAATG AGCCCTCTTT TACCAGTCCC AGCAGAATTT 
TTCCATCCTG ATCTCTCTGC AACTGAAACA GATCGAGAGA TTCCAAACAC CAGAGAAAAT 
ACTTCCAAAC TGCCATCTCA AGAAACGTGG GCAACTCTGC ACTCTCCAGA TATCAATTGC 
TCACTTCTTC GGCAGGCAAC AGAAAGCAAT TCATCTATGG AAAGTGGTAG TGCAACGTCT 
GAGGACAGTT TACAGACCAC ATTAGAAGAT AGCCTAAATC TTAGTGACTC ACCCCAGTGC 
ACATTGGATC TCCCAGATCT CCGAGCAACA CCTGCATCGC ACCATACTCC ATTGCCTATA 
TCATCTCTAT CCATGATGCA AAGGAGAAGT AACCGCCCTA GCTTTGCCCT TCAGAAAGTT 
CGACGTCACC AACGGAGCCA TAGCAGTGGT GGTAGCACTA GTCCTGGATG TACTCTTCAT 
GACTCCATGG ACCCTTCTGA TGAGGAAGGG GCAGGTGGAA GCCTGCGAGG TGTGAGCGAG 
CCCTCTGAGA CATTTAGCAG CTTATCTCTG ACCTCCCTCT TTTCACCACC TCCTTCTTTA 
GCACCACCCG GGGGCACAAT TAAAAAATGC AGCAGTGTCA ATAGTCTTTC TGTTGCTCCT 
TCTAGGGTAA GAGGAGCAAA GCAATTTTAC ACAGTTGATC CACAGGGCTT CCTTTCTATG 
CCATCTTGGG TTATGGATTT TTACCAAACT TCGACTCCCT CAGAAAACCA AGAGAATAGT 
AGCAGTACCG AAAAATTACC AACTATGGAA AAGGGGTTAC CACCCAGTAG TGTCTCAGGA 
AATGTGGATT TTGGCAATAA CAGTCGTCGA AACAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017948704.2
CDS	15..6233
Translation	MMVILLNCVTLGMFQPCEDVDCSSERCQILQVFDDFIFIFFAMEMVVKMVALGIFGKKCYLGDTWNRLDFFIVMAGMVEYSLDLQNINLSAIRTVRVLRPLKAINRVPSMRILVNLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWAGLLRNRCFLEENFTIQGDITLPPYYQPEEDDEMPFICSLSGDNGIMGCHEIPPLRDRGHECCLDKEDYYYYNSIRHEFNVSGMCVNWNQYYNVCRTSNTNPHKGAINFDNIGYAWIVIFQVITLEGWVEIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQREHQLMQEQRALYLSSSTVASYAEPGDCYEEIFQYVCHILRKAKRRTVGLYTSLQNRRQSRHELTNAKLGINGKEQHHLWICQKHGQLDYAPHAIVQPIAMTLTSDLSNCPQCTKTKWEDGRKLSAPNSTRSSREGTQRQRSESERESRSDGSTSDLDKEEEEDGDEGFCKGLCGEVRVKLRGIVDSKYFNRGIMIAILVNTISMGIEHHEQPEELTNILEISNIVFTSMFALEMMLKLSAFGLFDYLRNPYNIFDGIIVIISVWEIIGQNDGGLSVLRTFRLLRVLKLVRFMPALRRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHIFGCKFSLRTDSGDTVPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMASTSPWAALYFVALMTFGNYVLFNLLVAILVEGFQAEGDANRSYSDEERSSSNMEECDKYREAESPSDSKLCSVPMTPNGHLENSPIPTQQIPTTIMNTSRNSLQRDQVLVTVGCRKSSVMSLGRTSYDQRSQMGPRPSHWGRSSAWGSRRSSWNSLGRRHSIKPRTFTTEHESLLSRGEEQRERSHSRRSSSLKHRRAMSLDNKGSSDNPGDTVTHPNHHRGSCKGASMPEEHRESIGKTPPIMKEVFPKMNNRKDKEEEEEEIDYSLCFRIRKMIEVYRPDWCELREDWSIFLFSPQNRFRIMCQTIIAHKLFDYVVLAFIFLNCITIALERPQIEHKSTERLFLSISNYIFTAIFVAEMTLKVVSLGLYFGDQAYLRSSWNILDGFLVFVSLIDIVVSVASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLKPIGNIVLICCAFFIIFGILGVQLFKGKFFYCLGVDVRNVTNKSDCEAANYKWVHHKYNFDNLGQALMSLFVLASKDGWVNIMYNGLDAVAVDQQPVTNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLEKKRRKAQRLPYYATYCPARLLIHSVCTSHYLDIFITIIICLNVITMSLEHYSQPRSLEIALKYCNYMFTTVFVLEAVLKLVAFGFRRFFKDRWNQLDLAIVLLSVMGITLEEIEINAALPINPTIIRIMRVLRITRVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGKLVCNDDHPCEGMSRHATFENFGMAFLTLFQVSTGDNWNGIMKDTLRDCTSDDRSCLSNLQFISPLYFVSFVLTAQFVLINVVVAVLMKHLDDSNKEAQEDAELDAEIEMELAHGLCSQTSPVSGLDNGGRGGRPATDSHRSLRCFSPAQENLWLDSVSLIIKDSFEGELTIIDNLSGSVFHHYSSPAVCEKCNHDKQEVQLAEMEAFSLTSDKSSSVLTDELSSEDQGVTSQLKEMKDGQDSPDSLGMFGENIFPLASIPGSPTAEHFMCEFEKVSLTPAESWESWLKEESSPVSPSPLCADTMSPLLPVPAEFFHPDLSATETDREIPNTRENTSKLPSQETWATLHSPDINCSLLRQATESNSSMESGSATSEDSLQTTLEDSLNLSDSPQCTLDLPDLRATPASHHTPLPISSLSMMQRRSNRPSFALQKVRRHQRSHSSGGSTSPGCTLHDSMDPSDEEGAGGSLRGVSEPSETFSSLSLTSLFSPPPSLAPPGGTIKKCSSVNSLSVAPSRVRGAKQFYTVDPQGFLSMPSWVMDFYQTSTPSENQENSSSTEKLPTMEKGLPPSSVSGNVDFGNNSRRNR

Target ORF information:

RefSeq Version	XM_018093215.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calcium channel, voltage-dependent, T type, alpha 1I subunit (cacna1i), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018093215.2

ORF Insert Sequence:

ATGATGGTAA TTCTTCTGAA CTGTGTAACC CTCGGAATGT TCCAGCCTTG TGAAGATGTC 
GACTGCTCAT CAGAGCGATG CCAAATTCTT CAAGTCTTCG ATGACTTTAT ATTCATCTTC 
TTTGCAATGG AGATGGTCGT GAAAATGGTG GCCTTGGGCA TCTTTGGGAA GAAGTGTTAC 
CTTGGAGATA CATGGAATAG GTTGGACTTC TTTATAGTCA TGGCTGGGAT GGTGGAATAT 
TCTCTTGATC TTCAGAACAT TAATCTATCG GCCATCCGCA CTGTGCGTGT TCTGAGACCC 
CTAAAAGCCA TCAACCGAGT ACCCAGCATG CGGATCCTGG TGAATTTGCT GCTGGACACA 
TTGCCTATGC TGGGGAATGT ACTTCTGCTT TGCTTTTTTG TCTTCTTCAT TTTTGGGATC 
ATTGGGGTGC AGCTTTGGGC CGGATTGCTG CGAAACCGCT GCTTTTTGGA AGAAAACTTC 
ACTATTCAAG GGGATATAAC CTTACCACCT TACTACCAGC CAGAAGAAGA TGATGAAATG 
CCATTTATTT GTTCTTTGTC TGGTGACAAT GGAATCATGG GGTGCCATGA AATCCCCCCA 
TTAAGAGACC GCGGTCATGA GTGCTGTTTG GACAAGGAGG ATTATTATTA TTATAACTCT 
ATCCGTCACG AATTTAATGT CAGCGGCATG TGTGTTAATT GGAACCAATA CTATAACGTC 
TGCCGCACCA GCAACACCAA TCCTCACAAG GGCGCCATCA ATTTTGATAA CATTGGCTAT 
GCCTGGATTG TCATATTCCA GGTTATCACT CTGGAAGGCT GGGTAGAGAT AATGTACTAT 
GTCATGGATG CCCACTCCTT TTACAACTTC ATCTATTTCA TTCTTCTTAT TATAGTGGGA 
TCATTTTTCA TGATCAATTT ATGCCTGGTT GTTATTGCTA CACAATTCTC GGAGACCAAG 
CAGAGGGAGC ACCAGCTGAT GCAGGAGCAG AGGGCTCTCT ATTTATCCTC TAGTACTGTA 
GCCAGTTATG CTGAGCCTGG CGACTGCTAC GAGGAGATCT TTCAATATGT GTGCCATATC 
CTGCGCAAGG CCAAGCGCCG TACAGTAGGC CTCTACACTA GCCTTCAAAA CCGACGGCAA 
AGTCGCCATG AGCTGACCAA TGCTAAGCTT GGGATCAATG GCAAGGAACA GCATCATCTG 
TGGATATGTC AGAAACATGG CCAGCTGGAC TATGCTCCTC ATGCTATTGT GCAGCCTATC 
GCAATGACCT TAACGTCTGA CCTCTCCAAT TGCCCACAAT GCACCAAAAC CAAATGGGAG 
GATGGCCGTA AGCTATCAGC ACCAAACAGC ACCAGATCAA GCCGAGAGGG GACTCAGAGA 
CAGAGAAGTG AGAGCGAAAG GGAGAGCAGG AGTGATGGAA GCACATCCGA TCTAGACAAA 
GAAGAGGAGG AGGACGGTGA TGAAGGCTTC TGTAAAGGAC TCTGTGGCGA AGTCCGTGTA 
AAGCTCAGAG GAATCGTAGA TAGTAAATAT TTTAATCGGG GAATTATGAT TGCCATTCTT 
GTGAACACAA TCAGTATGGG AATAGAACAT CATGAGCAGC CAGAAGAACT AACAAATATC 
CTGGAAATCA GTAACATTGT CTTTACCAGC ATGTTTGCTC TGGAAATGAT GCTGAAGCTC 
TCAGCCTTTG GCCTCTTTGA TTACCTGCGA AACCCATATA ACATCTTTGA TGGCATCATT 
GTCATAATCA GTGTCTGGGA GATAATTGGG CAGAATGATG GAGGGCTGTC TGTACTGAGA 
ACTTTCCGAC TTCTGCGAGT ACTGAAGCTG GTCCGGTTTA TGCCTGCCTT AAGGCGTCAG 
TTGGTGGTTC TGATGAAGAC TATGGACAAC GTGGCCACAT TCTGCATGCT TCTGATGCTC 
TTCATCTTCA TTTTCAGTAT CCTTGGGATG CATATCTTTG GATGTAAATT CAGTTTAAGA 
ACTGATTCTG GAGACACTGT TCCAGATAGG AAAAACTTTG ATTCGCTACT GTGGGCCATT 
GTCACGGTTT TCCAGATCCT TACCCAAGAG GACTGGAATG TGGTGCTCTA CAATGGAATG 
GCCAGTACAT CTCCCTGGGC TGCGCTTTAC TTTGTAGCAT TAATGACGTT TGGGAATTAC 
GTTTTATTCA ACTTGCTTGT CGCTATATTG GTGGAAGGGT TTCAAGCAGA GGGAGATGCC 
AACAGATCTT ATTCTGATGA GGAAAGGAGT TCCTCCAATA TGGAGGAATG TGATAAATAT 
CGGGAAGCTG AATCTCCATC TGATTCCAAG CTTTGCTCAG TACCTATGAC TCCAAATGGA 
CATTTAGAAA ACAGCCCTAT TCCGACCCAA CAAATCCCCA CTACCATAAT GAATACATCA 
AGGAATTCTC TTCAAAGGGA CCAAGTTCTT GTAACTGTTG GCTGCAGAAA GAGTAGCGTG 
ATGTCCCTGG GGAGAACGTC CTACGACCAG AGGTCACAGA TGGGGCCTCG ACCCTCACAC 
TGGGGAAGAA GCAGTGCATG GGGGAGCCGC AGATCAAGCT GGAACAGTTT AGGGCGTCGT 
CACAGTATAA AGCCAAGAAC ATTCACAACT GAACATGAAT CATTGCTGTC ACGTGGGGAG 
GAACAGAGGG AAAGAAGTCA CTCCAGGAGA TCCTCCAGCC TAAAGCACAG AAGAGCAATG 
TCTCTAGATA ATAAAGGCTC TTCAGATAAC CCAGGGGATA CAGTAACTCA TCCCAACCAT 
CATAGAGGCT CTTGCAAAGG AGCATCTATG CCAGAAGAAC ATCGTGAATC CATTGGGAAA 
ACTCCACCAA TCATGAAGGA AGTGTTTCCC AAAATGAATA ACCGCAAAGA TAAGGAAGAG 
GAAGAAGAAG AAATAGATTA TAGTCTGTGC TTCCGGATCC GGAAAATGAT TGAAGTGTAC 
AGACCAGACT GGTGCGAGTT ACGTGAGGAC TGGTCTATAT TTCTATTCTC CCCACAGAAC 
AGGTTCCGCA TCATGTGCCA AACAATAATT GCGCACAAGC TTTTTGACTA TGTGGTCCTT 
GCCTTCATCT TCCTCAACTG CATTACTATC GCCTTGGAGA GGCCTCAGAT AGAGCACAAG 
AGTACGGAAA GGCTGTTCCT GAGTATATCA AATTATATCT TCACAGCCAT TTTTGTAGCT 
GAAATGACAT TGAAGGTGGT CTCTCTGGGA CTTTACTTTG GGGATCAAGC TTACCTCCGG 
AGCAGCTGGA ATATTCTGGA TGGGTTTCTC GTCTTTGTAT CCCTCATTGA TATTGTGGTG 
TCTGTAGCTT CAGCCGGTGG AGCAAAAATT CTGGGTGTCT TAAGGGTTCT TCGGCTTTTG 
AGGACATTGC GACCTCTCAG GGTTATCAGT AGAGCCCCTG GCCTGAAGCT GGTGGTGGAG 
ACCCTGATTT CTTCTCTAAA ACCCATTGGA AACATAGTTC TGATCTGCTG TGCCTTCTTT 
ATTATTTTTG GAATCCTTGG TGTGCAGCTT TTTAAGGGTA AATTTTTCTA CTGCCTTGGG 
GTGGACGTTC GAAATGTCAC CAATAAGTCA GACTGTGAGG CGGCCAATTA TAAATGGGTG 
CACCATAAGT ACAATTTCGA TAACCTGGGG CAGGCATTAA TGTCGCTTTT TGTCCTGGCA 
TCAAAGGACG GATGGGTTAA TATAATGTAT AATGGACTTG ATGCCGTGGC AGTGGACCAA 
CAGCCAGTGA CCAATCACAA CCCATGGATG TTGCTGTACT TTATCTCATT TCTGCTGATC 
GTCAGTTTCT TTGTACTGAA CATGTTTGTG GGTGTCGTGG TTGAGAACTT TCACAAATGT 
CGGCAGCATC AGGAAGCCGA GGAGGCCCGA AGACGAGAAG AAAAACGCTT GAGGCGCCTG 
GAGAAAAAAC GAAGGAAAGC CCAGCGCTTA CCGTACTATG CCACCTATTG TCCTGCTCGC 
CTCCTTATTC ATTCCGTCTG CACCAGCCAC TACTTGGATA TTTTCATTAC AATCATCATT 
TGCCTTAACG TTATCACAAT GTCCTTGGAG CACTATAGTC AACCAAGGTC TCTTGAAATT 
GCTCTAAAGT ACTGTAACTA CATGTTTACT ACAGTCTTTG TACTTGAAGC TGTGCTGAAG 
CTGGTGGCCT TTGGATTCAG ACGTTTCTTC AAGGACAGGT GGAACCAACT GGACCTGGCC 
ATTGTGCTCC TGTCTGTGAT GGGAATCACT TTAGAAGAAA TTGAAATAAA TGCTGCTTTG 
CCCATCAATC CCACCATTAT TCGCATCATG AGGGTGCTGC GCATCACCAG GGTGCTGAAG 
CTGCTTAAAA TGGCAACAGG CATGAGGGCG CTACTGGACA CTGTGGTACA AGCTTTGCCT 
CAGGTAGGAA ACTTGGGACT GCTCTTCATG CTTCTCTTCT TCATCTATGC TGCTCTGGGA 
GTGGAGCTCT TCGGGAAGCT TGTTTGCAAT GATGATCACC CGTGTGAAGG AATGAGTCGC 
CACGCCACAT TTGAGAATTT TGGAATGGCC TTCCTGACCC TCTTTCAGGT TTCTACTGGG 
GACAACTGGA ATGGAATAAT GAAGGATACC TTGCGTGACT GCACCAGTGA TGATCGCAGT 
TGCTTGAGCA ACCTCCAGTT CATCTCGCCT TTGTACTTTG TGAGCTTTGT GCTCACAGCT 
CAGTTTGTGC TAATTAACGT AGTAGTCGCT GTGTTAATGA AACACCTGGA TGACAGCAAT 
AAAGAAGCGC AGGAGGACGC TGAGCTGGAT GCGGAGATTG AAATGGAGTT GGCTCATGGC 
CTCTGTTCTC AAACATCGCC GGTCTCTGGT TTAGATAATG GAGGACGCGG AGGAAGACCT 
GCAACTGACA GTCATCGTAG CTTAAGGTGT TTTTCTCCTG CTCAGGAGAA TTTGTGGTTG 
GACAGTGTGT CCTTAATCAT TAAGGATTCC TTTGAAGGAG AGCTAACAAT CATTGATAAT 
TTATCTGGAT CAGTCTTCCA CCATTACTCC TCCCCAGCCG TCTGTGAGAA GTGCAACCAC 
GACAAACAAG AGGTCCAGCT GGCAGAAATG GAGGCCTTTT CTTTAACCTC TGACAAGTCC 
TCGTCAGTAC TGACTGATGA ACTCAGTTCA GAAGATCAAG GTGTCACGAG TCAGTTAAAG 
GAGATGAAGG ATGGGCAAGA TAGTCCTGAT TCCTTGGGAA TGTTTGGAGA AAATATCTTC 
CCACTTGCAA GCATTCCCGG TTCCCCAACA GCGGAGCACT TTATGTGTGA GTTTGAGAAG 
GTCTCATTAA CCCCGGCTGA GTCATGGGAA AGCTGGCTGA AGGAAGAGAG CAGCCCAGTC 
TCTCCTTCCC CGCTATGTGC GGATACAATG AGCCCTCTTT TACCAGTCCC AGCAGAATTT 
TTCCATCCTG ATCTCTCTGC AACTGAAACA GATCGAGAGA TTCCAAACAC CAGAGAAAAT 
ACTTCCAAAC TGCCATCTCA AGAAACGTGG GCAACTCTGC ACTCTCCAGA TATCAATTGC 
TCACTTCTTC GGCAGGCAAC AGAAAGCAAT TCATCTATGG AAAGTGGTAG TGCAACGTCT 
GAGGACAGTT TACAGACCAC ATTAGAAGAT AGCCTAAATC TTAGTGACTC ACCCCAGTGC 
ACATTGGATC TCCCAGATCT CCGAGCAACA CCTGCATCGC ACCATACTCC ATTGCCTATA 
TCATCTCTAT CCATGATGCA AAGGAGAAGT AACCGCCCTA GCTTTGCCCT TCAGAAAGTT 
CGACGTCACC AACGGAGCCA TAGCAGTGGT GGTAGCACTA GTCCTGGATG TACTCTTCAT 
GACTCCATGG ACCCTTCTGA TGAGGAAGGG GCAGGTGGAA GCCTGCGAGG TGTGAGCGAG 
CCCTCTGAGA CATTTAGCAG CTTATCTCTG ACCTCCCTCT TTTCACCACC TCCTTCTTTA 
GCACCACCCG GGGGCACAAT TAAAAAATGC AGCAGTGTCA ATAGTCTTTC TGTTGCTCCT 
TCTAGGGTAA GAGGAGCAAA GCAATTTTAC ACAGTTGATC CACAGGGCTT CCTTTCTATG 
CCATCTTGGG TTATGGATTT TTACCAAACT TCGACTCCCT CAGAAAACCA AGAGAATAGT 
AGCAGTACCG AAAAATTACC AACTATGGAA AAGGGGTTAC CACCCAGTAG TGTCTCAGGA 
AATGTGGATT TTGGCAATAA CAGTCGTCGA AACAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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cacna1i cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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