cacna1i cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	cacna1i
Entrez Gene ID	566351
Full Name	calcium channel, voltage-dependent, T type, alpha 1I subunit
Synonyms	si:ch211-51h13.1
General protein information	Preferred Names calcium channel, voltage-dependent, T type, alpha 1I subunit Names voltage-dependent T-type calcium channel subunit alpha-1I
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009299474.2	XP_009297749.1	voltage-dependent T-type calcium channel subunit alpha-1I
XM_009299474.3	XP_009297749.2	voltage-dependent T-type calcium channel subunit alpha-1I isoform X1
XM_021473957.1	XP_021329632.1	voltage-dependent T-type calcium channel subunit alpha-1I isoform X2

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	CACNA1I	XP_425474.3
Pan troglodytes (chimpanzee)	CACNA1I	XP_515143.4
Canis lupus familiaris (dog)	CACNA1I	XP_538364.4
Homo sapiens (human)	CACNA1I	NP_001003406.1
Bos taurus (cattle)	CACNA1I	XP_002687981.2
Mus musculus (house mouse)	Cacna1i	NP_001037773.2
Xenopus tropicalis (tropical clawed frog)	cacna1i	XP_002933803.2
Rattus norvegicus (Norway rat)	Cacna1i	NP_064469.3
Danio rerio (zebrafish)	cacna1i	XP_694715.5

Gene Ontology

The following cacna1i gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cacna1i cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa141759	XM_009299474.3 Latest version!	Danio rerio calcium channel, voltage-dependent, T type, alpha 1I subunit (cacna1i), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ODa141760	XM_021473957.1 Latest version!	Danio rerio calcium channel, voltage-dependent, T type, alpha 1I subunit (cacna1i), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ODa44854	XM_009299474.2 Latest version!	Danio rerio calcium channel, voltage-dependent, T type, alpha 1I subunit (cacna1i), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cacna1i ( XM_009299474.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009297749.2 Danio rerio calcium channel, voltage-dependent, T type, alpha 1I subunit (cacna1i), transcript variant X1, mRNA.

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CloneID	ODa141759
Clone ID Related Accession (Same CDS sequence)	XM_009299474.3
Accession Version	XM_009299474.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent T-type calcium channel subunit alpha-1I isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009299474.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGTTG GCCTTAACTC TTGCTTCCTC ACGTGGTTCG AGCGCATCAG TATAATGGTG 
ATCCTGCTGA ACTGCGTCAC TCTGGGGATG TACCAGCCTT GCGAGAACAT CGACTGCACT 
TCAGAGCGCT GTCAGGTCCT ACAGGCTTTT GATGCATTTA TCTACATTTT CTTTGCACTT 
GAGATGGTGG TGAAGATGGT GGCTCTGGGG ATCTTTGGCC GTCGCTGCTA TCTTGGCGAT 
ACTTGGAACA GGCTGGACTT TTTCATCGTG ATGGCAGGGA TGGTGGAATA CTCCCTTGAT 
CTCCAGAACA TCAACTTCTC CGCCATCAGA ACAGTGCGTG TGCTAAGACC TCTCAAAGCC 
ATCAACAGAG TACCCAGTAT GCGCATCCTG GTGAACCTGC TGCTGGACAC TCTGCCCATG 
CTGGGAAATG TGCTCCTGCT TTGTTTCTTT GTGTTCTTCA TTTTTGGCAT CATTGGTGTA 
CAACTGTGGG CTGGACTCCT AAGAAATCGG TGTTACCCAG AGGAGAACTT CACCCTCACA 
TCTGGCCTCA CCCTTCCCAC ACCATATTAT CAGCCTGAGG AGGATGACGA GCGGCCCTTC 
ATCTGCTCGT TGGCTCAAGA TAACGGCATC ATGTCCTGCA GCGATGTGCC TGCTCGGAGA 
GAGGGCGGAC GGGAGTGCTG TCTGGATAAA GAGGACGCTC TTCACCGGCA GGCTCTGGGT 
CTGAGCGCCG AGCCGCTGGT CAACGGCAGC GCATCTGCCA TGGGCCTCTG TGTGAACTGG 
AATCAGTACT ACACCCGCTG CCACACTGGA CACACCAATC CTCATAAAGG AGCAATTAAT 
TTCGACAACA TAGGATACGC ATGGATCGTC ATATTTCAGG TGATCACGTT AGAGGGCTGG 
GTGGAAATCA TGTACTACGT CATGGACGCG CATTCCTTCT ACAACTTCAT CTATTTTATA 
TTCCTCATCA TCATTGGATC TTTCTTCATG ATTAATCTGT GCCTGGTGGT CATTGCCACT 
CAGTTTTCGG AGACCAAACA GCGAGAGCAC CAGCTGATGC AAGAGCAACG TGCTCGCTAC 
CTGTCCTCCA GCACGCTGGC TTCACTCGCT GAGCCCGGAG ACTGTTACGA GGAGCTCTTC 
CAACTGGTCT GCCATATCCT ACGCAAAGCC CGCCGCCGCT CTGCTGCCCT TTATTACATG 
CTCCGTGGCA AAGCCCCTCC CCCTGGAGGT GGGAGGGGGC GTGGCAAAGG AGGAGCAGGA 
TCGATAGGAG GAGGAGCCAA TGTTAATGGA GGAGAGAAAC ATCACTGCCA TTCAGCTCAG 
ACATCTCACT GTATTCACGA AACCAAATTG GACCACAGCT CAGAGAATGC AGTCTCCCTC 
TCCATCAGTC CAAACCCAGA GGAGTGTCCA CAGTGTGCTG CAGCCCTGTC TCTCAAAGAG 
GGGGAGGAGT CAACAGGGCA TTCAGCCAAT GGGGAGGAAG AGGAGGGTGC GCTGGAGGAA 
ACTGACAGGG ACGAGAACCG CTTAGACAAA AAGAGGAGCA GCGACTCTGA TGACACGCTA 
AAGAAGAAAC GCACCTGTTT GGGGAAATGT AAAGACGTCT GGGACGAAAT AAGAGTGAAA 
CTTTGGGGCA TCGTTGAAAG CAAGTACTTC AACAGAGGGA TTATGATTGC CATCCTCATC 
AATACAATCA GCATGGGCAT CGAGCACCAT AACCAGCCTG ATGAACTGAC TAATGTCCTG 
GAGATCTGTA ACATAGTGTT CACCAGTATG TTCACTCTGG AGATGATCCT CAAGCTCACT 
GCCTTTGGCT TCTTTGAATA TCTGAGAAAC CCTTACAACA TCTTTGACGG CATCATTGTG 
ATCATCAGTG TATGTGAGAT CATTGGTCAG TCTGACGGAG GTCTGTCAGT CCTGAGGACG 
TTCAGATTGC TGAGGGTGAT AAAGCTGGTG CGGTTCATGC CAGCTCTGCG CAGGCAGCTG 
GTGGTCCTGA TGAAGACCAT GGACAATGTG GCCACGTTCT GCATGCTGCT AATGCTCTTC 
ATCTTTATAT TCAGTATTCT GGGAATGCAC ATATTTGGCT GCAAGTTCAG CCTGAAGACA 
GAGGCTGGGG ACACCGTACC TGACAGGAAG AACTTTGACT CTCTGCTTTG GGCCATTGTT 
ACAGTGTTTC AGATCTTAAC TCAAGAGGAC TGGAACATGG TTTTGTATAA CGGCATGGCC 
TCCACCTCGC CTCTGGCAGC CCTGTATTTC GTCGCGCTCA TGACTTTTGG AAACTACGTT 
CTATTCAATC TGCTTGTGGC CATCTTGGTT GAAGGATTTC AGGCTGAGGG CGATGCTAAC 
CGTTCCTACT CGGATGATGA CAGATCATCT TGTAATTTAG AAGAAACCGA GAAAAAGGAC 
TCTCTCCAGC TGTCAGATCC CAAGATTTCC ACGTTGACCC CTAACGGGCA TCTGGATTTA 
GCACCTGCAC CCAATGCTAG GGGCTTTTAC CCATCTGAAA GGCTGAGCTT TGCCCTGGGT 
TCGCGCAAGA GCAGCGTTAG CTCTCTGGGC AGGGCTAGCC TGGAGCAAAC CACGCTGTGT 
CCAGGACGAG CATCTCTTTA CCACAATTGG GGACGCCCCG CACGGCCAGG AATATGGAGC 
CGCAGGTCGA GTTGGAACAG CTTGGGACGG TCCTCCAGAT GTCTGGGGAT GGGGGGTGGA 
GGCAGTTTAC GAGTCCGTAG CCCTCACTGT CATCCCGCCG AGCAGGAATC CCTGCTGTCT 
CCTCCACCTC CGCCTCTCCA TCCGCCTCTG CTCCCCAGAC ACTTTCCCCC ACGCAGGGAA 
CGCAGGGCTC TGTCTCTGGA GCTCCCAGAG CTTCTACAGG TGCCAGGACC ACCCCTGCCT 
CCTCTGCACC CCCGCCAGCG CAAGAAGAGC TTCTCTGGGG GTCTGGGAAG CGTTGGAGAG 
CATCAGGACT GTAATGGGAA AACGCCATCA GTTCAACCAC AAATCATTAA TGAAGTCTAC 
CCTCAGGTCA ACACACGCAA GGACAGGGAG GATCTAGAGG ATGAGCTAGA CTATAGTCTG 
TGTTTCCGGA TCCAGAAGAT GATGGAGGTG TACAGACCGG ACTGGTGTGA GACCAGGGAA 
GACTGGTCTG TCTTCCTCTT CTCTCCTCAA AACAAGTTCA GGTTGTTGTG TCAGTCCATC 
ATAGCCCACA AGCTCTTTGA CTATGTGGTG CTGGCCTTCA TCTTCTCAAA CTGCATCACT 
GTGGCTCTGG AGAGACCCAA GATCCTTCAG GGTAGCCTGG AGAGGCTTTT CCTTACCGTG 
TCCAATTACA TTTTCACAGC CATATTTGTG GGGGAGATGA CGCTCAAGGT GGTGTCTATG 
GGTTTGTATA TTGGAGAGCA GGCATACTTG CGCAGCAGCT GGAATATTTT GGATGGTTTC 
CTGGTGTTTG TGTCTTTGAT TGATATTGTG GTGTCGATGG CAGGCGGGGC TAAGATCCTG 
GGGGTGCTCA GGGTCCTCAG GCTTCTTCGC ACACTGCGCC CTCTCAGGGT TATCAGTCGA 
GCTCCTGGTC TCAAACTAGT TGTGGAGACT TTGATCACCT CTCTTAAGCC TATTGGAAAC 
ATAGTTCTCA TCTGCTGTGC CTTTTTCATT ATTTTTGGGA TCCTGGGAGT TCAGCTTTTC 
AAAGGCAAGT TCTACTACTG CTTGGGCCTG GATGTCAAAA ACATCACCAA CAAGTCTGAC 
TGCCTCTTGG CCAATTATAA GTGGGTCCAT CACAAGTACA ACTTTGACAA CTTGGGGCAG 
GCTCTGATGT CCTTGTTTGT ACTGGCTTCG AAGGACGGCT GGGTCAACAT CATGTATCAT 
GGTTTGGATG CTGTGGCAGT GGACCAACAG CCCATAACCA ACAACAACCC CTGGATGCTG 
CTGTACTTCA TCTCATTCCT CTTGATCGTC AGTTTCTTCG TGCTGAATAT GTTTGTTGGA 
GTGGTGGTGG AGAACTTCCA TAAATGCCGA CAGCATCAGG AGGTGGAGGA GGCCAAGAGG 
AGGGAGGAGA AGAGACAGAG ACGCATGGAG AAAAAGAGAC GGAAGGCCCA GAAGTTGCCC 
TACTATGCCA GTTATAGTCA TGTACGGCTG ATGATCCACA CCCTGTGCAC CAGTCACTAC 
CTGGATATCT TCATCACCTT CATCATCTGT GTGAATGTAG TCACCATGTC ACTGGAGCAC 
TACAGCCAGC CTCATTCTCT GGAGATCGCA CTGAAGTACT GTAATTATTT CTTCACCAGC 
ACCTTTGTGC TGGAGGCTGT CCTGAAGCTC ATCGCATTTG GCTTCCGACG TTTCTTCAAA 
GACAGGTGGA ACCAGCTGGA TTTGGCAATT GTACTGCTGT CTGTCATGGG CATCACGCTG 
GAGGAGATTG AAATCAGTGC AGCTCTGCCC ATCAACCCCA CCATAATACG TATCATGAGG 
GTCCTACGCA TCGCACGTGT GTTAAAGCTG TTAAAGATGG CCACAGGAAT GAGAGCGCTG 
CTGGATACAG TAGTTCAAGC CCTGCCACAG GTGGGGAACC TTGGCCTCCT CTTCATGCTG 
CTGTTCTTCA TCTATGCTGC ACTGGGGGTG GAATTGTTCG GAGAGCTGGT GTGCAATGAA 
GATTACCCAT GTGAAGGCAT GAGTCGTCAT GCCACCTTTG AGAACTTCGG CATGGCGTTC 
CTCACCCTTT TCCAGGTCTC CACTGGCGAT AACTGGAATG GCATAATGAA GGACACTCTC 
CGTGAATGTC CGCCAGGCGA ATACACCTGT AATCCCAGCC TACAATTCAT CTCCCCGCTG 
TACTTTGTGA GCTTTGTGCT CACGGCCCAG TTTGTCCTCA TCAATGTGGT GGTGGCGGTG 
CTGATGAAAC ACCTGGACGA CTCAAACAAG GAGGCCCAGG AGGAGGCAGA GATGGATGCC 
GAGATTGAAA TGGAGCTGGC CCAGGGAACG CTCTGCTGCA TCGGCGGAGG GGTAGCCGTA 
GCCGACAGGA CCGGCATGGG GCATCAGGGG GCTGCATCCT GCTCCGTTGT TCCCCCTCAC 
TCTCCAATCA CACACCCAGT GGCCCACGAA CCCAACAGCA TCAGGAGACT CTACTCACCG 
GCGCAGGAGA ACTTGTGGCT GGACAGTGTA TCTCTGCTCA TCAAAGATTC ATTTGAAGGA 
GAGATGCTGA TGATTGACAA CCTCTCTGGC TCCGTCTTCC ATCATTACTC CTCTCCTCCT 
GTCTGCAAGG ACTGCAGGAC TCATCCGCAA GAGCAGATCC ACATGGCTGA ACTGGAGCAG 
GCGTCTCTGA GGTCGGAGCA GCTCTCAGAT AAGTCATCCT CGCCAGCGCT GCCCGATGAC 
CTCAGCCTGG ACGAGCAGAG CGTCTACCAG ATGGCTGCCA AAGAGGGCAA GGAGAGAGTC 
TGTGATGAGT GCCATCATTC AGAAGAGCCA GGAGGGAGAG GAAACAGCAG GTTACGACGA 
TCCTCTCGCG TCCACAGCTC TGGGACAGAA GATGGAGGAA CTTGTCAAAG TCCACATCAC 
AACACACCTG CTCGACACAG CATAGGTGGC GTTCCCTGTA GCAGCAGTAG TCAAGAGCAC 
CCAGGGGTTT CTGGAGGTTC GAGCCGCTCT AACACCCCCG TTTGTCTCCC TGCTGAATTC 
TTCCACCCAG CTGCTGCGGC TCTGCCTGCA CCTCCGAGAG GCCGAACAGG ACACAAACCA 
AGAGGCCTTC GGCTAACTTC ACCAGCCTCT TGGGCTTCTC TGCGCTCTCC TGGAGCAAAC 
ACCAGGCTGC TCACCACACA GTACCCATCT CACAGTGACT CCTCTCTGGC TACGGGCAGC 
TCAGAGGGCA GCCTACCGAC CACCATGGAA GAAGGGCTCA GTTTCATCGT ATCAACGCCC 
CAGCCCCTTC TGGACACACT CCTCGACGAG CGTACTAGCC TCTCCACAGA GACCCTGAGA 
CCCAGTCCTC CAGCCACACA CATCCTCCAA ACCACTAGGG GGCACCAGCG CAGCAGGAGC 
AGCTGTGAAA CGGACATCAA TACGGGCAGC ATTCGACAAG GTTCCGAGGC GGATGTAGGA 
TTGGTATGGT GCAGCTCCGA GCAGACATGC GACAGCCAGC AGCTCTCAGA GACGCAGAGC 
AGCCTGTCGC TCACTTCGCT ACTTCTACCA AGCTCTCTAG TGCCCCTGTC TGTGAAAAAG 
TGCAACAGCA CGGGCAGCTT AGACCAGGGG ACTCTCACAG CCCAAGAAAA AGTGTTCACG 
AAAGAGCCAC AGGGTAATAT CACCAGCCCC TGGAAAGAGA GGAAGGAGAG GCAGTCGGAG 
GCAGCACAAG GAACAGATGG GGAAAGTACA AGCCAAATAA CAATTGCGGG CTCTAGGAAA 
AATAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009297749.2
CDS	1300..7668
Translation	MNVGLNSCFLTWFERISIMVILLNCVTLGMYQPCENIDCTSERCQVLQAFDAFIYIFFALEMVVKMVALGIFGRRCYLGDTWNRLDFFIVMAGMVEYSLDLQNINFSAIRTVRVLRPLKAINRVPSMRILVNLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWAGLLRNRCYPEENFTLTSGLTLPTPYYQPEEDDERPFICSLAQDNGIMSCSDVPARREGGRECCLDKEDALHRQALGLSAEPLVNGSASAMGLCVNWNQYYTRCHTGHTNPHKGAINFDNIGYAWIVIFQVITLEGWVEIMYYVMDAHSFYNFIYFIFLIIIGSFFMINLCLVVIATQFSETKQREHQLMQEQRARYLSSSTLASLAEPGDCYEELFQLVCHILRKARRRSAALYYMLRGKAPPPGGGRGRGKGGAGSIGGGANVNGGEKHHCHSAQTSHCIHETKLDHSSENAVSLSISPNPEECPQCAAALSLKEGEESTGHSANGEEEEGALEETDRDENRLDKKRSSDSDDTLKKKRTCLGKCKDVWDEIRVKLWGIVESKYFNRGIMIAILINTISMGIEHHNQPDELTNVLEICNIVFTSMFTLEMILKLTAFGFFEYLRNPYNIFDGIIVIISVCEIIGQSDGGLSVLRTFRLLRVIKLVRFMPALRRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHIFGCKFSLKTEAGDTVPDRKNFDSLLWAIVTVFQILTQEDWNMVLYNGMASTSPLAALYFVALMTFGNYVLFNLLVAILVEGFQAEGDANRSYSDDDRSSCNLEETEKKDSLQLSDPKISTLTPNGHLDLAPAPNARGFYPSERLSFALGSRKSSVSSLGRASLEQTTLCPGRASLYHNWGRPARPGIWSRRSSWNSLGRSSRCLGMGGGGSLRVRSPHCHPAEQESLLSPPPPPLHPPLLPRHFPPRRERRALSLELPELLQVPGPPLPPLHPRQRKKSFSGGLGSVGEHQDCNGKTPSVQPQIINEVYPQVNTRKDREDLEDELDYSLCFRIQKMMEVYRPDWCETREDWSVFLFSPQNKFRLLCQSIIAHKLFDYVVLAFIFSNCITVALERPKILQGSLERLFLTVSNYIFTAIFVGEMTLKVVSMGLYIGEQAYLRSSWNILDGFLVFVSLIDIVVSMAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLITSLKPIGNIVLICCAFFIIFGILGVQLFKGKFYYCLGLDVKNITNKSDCLLANYKWVHHKYNFDNLGQALMSLFVLASKDGWVNIMYHGLDAVAVDQQPITNNNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEVEEAKRREEKRQRRMEKKRRKAQKLPYYASYSHVRLMIHTLCTSHYLDIFITFIICVNVVTMSLEHYSQPHSLEIALKYCNYFFTSTFVLEAVLKLIAFGFRRFFKDRWNQLDLAIVLLSVMGITLEEIEISAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGELVCNEDYPCEGMSRHATFENFGMAFLTLFQVSTGDNWNGIMKDTLRECPPGEYTCNPSLQFISPLYFVSFVLTAQFVLINVVVAVLMKHLDDSNKEAQEEAEMDAEIEMELAQGTLCCIGGGVAVADRTGMGHQGAASCSVVPPHSPITHPVAHEPNSIRRLYSPAQENLWLDSVSLLIKDSFEGEMLMIDNLSGSVFHHYSSPPVCKDCRTHPQEQIHMAELEQASLRSEQLSDKSSSPALPDDLSLDEQSVYQMAAKEGKERVCDECHHSEEPGGRGNSRLRRSSRVHSSGTEDGGTCQSPHHNTPARHSIGGVPCSSSSQEHPGVSGGSSRSNTPVCLPAEFFHPAAAALPAPPRGRTGHKPRGLRLTSPASWASLRSPGANTRLLTTQYPSHSDSSLATGSSEGSLPTTMEEGLSFIVSTPQPLLDTLLDERTSLSTETLRPSPPATHILQTTRGHQRSRSSCETDINTGSIRQGSEADVGLVWCSSEQTCDSQQLSETQSSLSLTSLLLPSSLVPLSVKKCNSTGSLDQGTLTAQEKVFTKEPQGNITSPWKERKERQSEAAQGTDGESTSQITIAGSRKNR

Target ORF information:

RefSeq Version	XM_009299474.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, T type, alpha 1I subunit (cacna1i), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299474.3

ORF Insert Sequence:

ATGAATGTTG GCCTTAACTC TTGCTTCCTC ACGTGGTTCG AGCGCATCAG TATAATGGTG 
ATCCTGCTGA ACTGCGTCAC TCTGGGGATG TACCAGCCTT GCGAGAACAT CGACTGCACT 
TCAGAGCGCT GTCAGGTCCT ACAGGCTTTT GATGCATTTA TCTACATTTT CTTTGCACTT 
GAGATGGTGG TGAAGATGGT GGCTCTGGGG ATCTTTGGCC GTCGCTGCTA TCTTGGCGAT 
ACTTGGAACA GGCTGGACTT TTTCATCGTG ATGGCAGGGA TGGTGGAATA CTCCCTTGAT 
CTCCAGAACA TCAACTTCTC CGCCATCAGA ACAGTGCGTG TGCTAAGACC TCTCAAAGCC 
ATCAACAGAG TACCCAGTAT GCGCATCCTG GTGAACCTGC TGCTGGACAC TCTGCCCATG 
CTGGGAAATG TGCTCCTGCT TTGTTTCTTT GTGTTCTTCA TTTTTGGCAT CATTGGTGTA 
CAACTGTGGG CTGGACTCCT AAGAAATCGG TGTTACCCAG AGGAGAACTT CACCCTCACA 
TCTGGCCTCA CCCTTCCCAC ACCATATTAT CAGCCTGAGG AGGATGACGA GCGGCCCTTC 
ATCTGCTCGT TGGCTCAAGA TAACGGCATC ATGTCCTGCA GCGATGTGCC TGCTCGGAGA 
GAGGGCGGAC GGGAGTGCTG TCTGGATAAA GAGGACGCTC TTCACCGGCA GGCTCTGGGT 
CTGAGCGCCG AGCCGCTGGT CAACGGCAGC GCATCTGCCA TGGGCCTCTG TGTGAACTGG 
AATCAGTACT ACACCCGCTG CCACACTGGA CACACCAATC CTCATAAAGG AGCAATTAAT 
TTCGACAACA TAGGATACGC ATGGATCGTC ATATTTCAGG TGATCACGTT AGAGGGCTGG 
GTGGAAATCA TGTACTACGT CATGGACGCG CATTCCTTCT ACAACTTCAT CTATTTTATA 
TTCCTCATCA TCATTGGATC TTTCTTCATG ATTAATCTGT GCCTGGTGGT CATTGCCACT 
CAGTTTTCGG AGACCAAACA GCGAGAGCAC CAGCTGATGC AAGAGCAACG TGCTCGCTAC 
CTGTCCTCCA GCACGCTGGC TTCACTCGCT GAGCCCGGAG ACTGTTACGA GGAGCTCTTC 
CAACTGGTCT GCCATATCCT ACGCAAAGCC CGCCGCCGCT CTGCTGCCCT TTATTACATG 
CTCCGTGGCA AAGCCCCTCC CCCTGGAGGT GGGAGGGGGC GTGGCAAAGG AGGAGCAGGA 
TCGATAGGAG GAGGAGCCAA TGTTAATGGA GGAGAGAAAC ATCACTGCCA TTCAGCTCAG 
ACATCTCACT GTATTCACGA AACCAAATTG GACCACAGCT CAGAGAATGC AGTCTCCCTC 
TCCATCAGTC CAAACCCAGA GGAGTGTCCA CAGTGTGCTG CAGCCCTGTC TCTCAAAGAG 
GGGGAGGAGT CAACAGGGCA TTCAGCCAAT GGGGAGGAAG AGGAGGGTGC GCTGGAGGAA 
ACTGACAGGG ACGAGAACCG CTTAGACAAA AAGAGGAGCA GCGACTCTGA TGACACGCTA 
AAGAAGAAAC GCACCTGTTT GGGGAAATGT AAAGACGTCT GGGACGAAAT AAGAGTGAAA 
CTTTGGGGCA TCGTTGAAAG CAAGTACTTC AACAGAGGGA TTATGATTGC CATCCTCATC 
AATACAATCA GCATGGGCAT CGAGCACCAT AACCAGCCTG ATGAACTGAC TAATGTCCTG 
GAGATCTGTA ACATAGTGTT CACCAGTATG TTCACTCTGG AGATGATCCT CAAGCTCACT 
GCCTTTGGCT TCTTTGAATA TCTGAGAAAC CCTTACAACA TCTTTGACGG CATCATTGTG 
ATCATCAGTG TATGTGAGAT CATTGGTCAG TCTGACGGAG GTCTGTCAGT CCTGAGGACG 
TTCAGATTGC TGAGGGTGAT AAAGCTGGTG CGGTTCATGC CAGCTCTGCG CAGGCAGCTG 
GTGGTCCTGA TGAAGACCAT GGACAATGTG GCCACGTTCT GCATGCTGCT AATGCTCTTC 
ATCTTTATAT TCAGTATTCT GGGAATGCAC ATATTTGGCT GCAAGTTCAG CCTGAAGACA 
GAGGCTGGGG ACACCGTACC TGACAGGAAG AACTTTGACT CTCTGCTTTG GGCCATTGTT 
ACAGTGTTTC AGATCTTAAC TCAAGAGGAC TGGAACATGG TTTTGTATAA CGGCATGGCC 
TCCACCTCGC CTCTGGCAGC CCTGTATTTC GTCGCGCTCA TGACTTTTGG AAACTACGTT 
CTATTCAATC TGCTTGTGGC CATCTTGGTT GAAGGATTTC AGGCTGAGGG CGATGCTAAC 
CGTTCCTACT CGGATGATGA CAGATCATCT TGTAATTTAG AAGAAACCGA GAAAAAGGAC 
TCTCTCCAGC TGTCAGATCC CAAGATTTCC ACGTTGACCC CTAACGGGCA TCTGGATTTA 
GCACCTGCAC CCAATGCTAG GGGCTTTTAC CCATCTGAAA GGCTGAGCTT TGCCCTGGGT 
TCGCGCAAGA GCAGCGTTAG CTCTCTGGGC AGGGCTAGCC TGGAGCAAAC CACGCTGTGT 
CCAGGACGAG CATCTCTTTA CCACAATTGG GGACGCCCCG CACGGCCAGG AATATGGAGC 
CGCAGGTCGA GTTGGAACAG CTTGGGACGG TCCTCCAGAT GTCTGGGGAT GGGGGGTGGA 
GGCAGTTTAC GAGTCCGTAG CCCTCACTGT CATCCCGCCG AGCAGGAATC CCTGCTGTCT 
CCTCCACCTC CGCCTCTCCA TCCGCCTCTG CTCCCCAGAC ACTTTCCCCC ACGCAGGGAA 
CGCAGGGCTC TGTCTCTGGA GCTCCCAGAG CTTCTACAGG TGCCAGGACC ACCCCTGCCT 
CCTCTGCACC CCCGCCAGCG CAAGAAGAGC TTCTCTGGGG GTCTGGGAAG CGTTGGAGAG 
CATCAGGACT GTAATGGGAA AACGCCATCA GTTCAACCAC AAATCATTAA TGAAGTCTAC 
CCTCAGGTCA ACACACGCAA GGACAGGGAG GATCTAGAGG ATGAGCTAGA CTATAGTCTG 
TGTTTCCGGA TCCAGAAGAT GATGGAGGTG TACAGACCGG ACTGGTGTGA GACCAGGGAA 
GACTGGTCTG TCTTCCTCTT CTCTCCTCAA AACAAGTTCA GGTTGTTGTG TCAGTCCATC 
ATAGCCCACA AGCTCTTTGA CTATGTGGTG CTGGCCTTCA TCTTCTCAAA CTGCATCACT 
GTGGCTCTGG AGAGACCCAA GATCCTTCAG GGTAGCCTGG AGAGGCTTTT CCTTACCGTG 
TCCAATTACA TTTTCACAGC CATATTTGTG GGGGAGATGA CGCTCAAGGT GGTGTCTATG 
GGTTTGTATA TTGGAGAGCA GGCATACTTG CGCAGCAGCT GGAATATTTT GGATGGTTTC 
CTGGTGTTTG TGTCTTTGAT TGATATTGTG GTGTCGATGG CAGGCGGGGC TAAGATCCTG 
GGGGTGCTCA GGGTCCTCAG GCTTCTTCGC ACACTGCGCC CTCTCAGGGT TATCAGTCGA 
GCTCCTGGTC TCAAACTAGT TGTGGAGACT TTGATCACCT CTCTTAAGCC TATTGGAAAC 
ATAGTTCTCA TCTGCTGTGC CTTTTTCATT ATTTTTGGGA TCCTGGGAGT TCAGCTTTTC 
AAAGGCAAGT TCTACTACTG CTTGGGCCTG GATGTCAAAA ACATCACCAA CAAGTCTGAC 
TGCCTCTTGG CCAATTATAA GTGGGTCCAT CACAAGTACA ACTTTGACAA CTTGGGGCAG 
GCTCTGATGT CCTTGTTTGT ACTGGCTTCG AAGGACGGCT GGGTCAACAT CATGTATCAT 
GGTTTGGATG CTGTGGCAGT GGACCAACAG CCCATAACCA ACAACAACCC CTGGATGCTG 
CTGTACTTCA TCTCATTCCT CTTGATCGTC AGTTTCTTCG TGCTGAATAT GTTTGTTGGA 
GTGGTGGTGG AGAACTTCCA TAAATGCCGA CAGCATCAGG AGGTGGAGGA GGCCAAGAGG 
AGGGAGGAGA AGAGACAGAG ACGCATGGAG AAAAAGAGAC GGAAGGCCCA GAAGTTGCCC 
TACTATGCCA GTTATAGTCA TGTACGGCTG ATGATCCACA CCCTGTGCAC CAGTCACTAC 
CTGGATATCT TCATCACCTT CATCATCTGT GTGAATGTAG TCACCATGTC ACTGGAGCAC 
TACAGCCAGC CTCATTCTCT GGAGATCGCA CTGAAGTACT GTAATTATTT CTTCACCAGC 
ACCTTTGTGC TGGAGGCTGT CCTGAAGCTC ATCGCATTTG GCTTCCGACG TTTCTTCAAA 
GACAGGTGGA ACCAGCTGGA TTTGGCAATT GTACTGCTGT CTGTCATGGG CATCACGCTG 
GAGGAGATTG AAATCAGTGC AGCTCTGCCC ATCAACCCCA CCATAATACG TATCATGAGG 
GTCCTACGCA TCGCACGTGT GTTAAAGCTG TTAAAGATGG CCACAGGAAT GAGAGCGCTG 
CTGGATACAG TAGTTCAAGC CCTGCCACAG GTGGGGAACC TTGGCCTCCT CTTCATGCTG 
CTGTTCTTCA TCTATGCTGC ACTGGGGGTG GAATTGTTCG GAGAGCTGGT GTGCAATGAA 
GATTACCCAT GTGAAGGCAT GAGTCGTCAT GCCACCTTTG AGAACTTCGG CATGGCGTTC 
CTCACCCTTT TCCAGGTCTC CACTGGCGAT AACTGGAATG GCATAATGAA GGACACTCTC 
CGTGAATGTC CGCCAGGCGA ATACACCTGT AATCCCAGCC TACAATTCAT CTCCCCGCTG 
TACTTTGTGA GCTTTGTGCT CACGGCCCAG TTTGTCCTCA TCAATGTGGT GGTGGCGGTG 
CTGATGAAAC ACCTGGACGA CTCAAACAAG GAGGCCCAGG AGGAGGCAGA GATGGATGCC 
GAGATTGAAA TGGAGCTGGC CCAGGGAACG CTCTGCTGCA TCGGCGGAGG GGTAGCCGTA 
GCCGACAGGA CCGGCATGGG GCATCAGGGG GCTGCATCCT GCTCCGTTGT TCCCCCTCAC 
TCTCCAATCA CACACCCAGT GGCCCACGAA CCCAACAGCA TCAGGAGACT CTACTCACCG 
GCGCAGGAGA ACTTGTGGCT GGACAGTGTA TCTCTGCTCA TCAAAGATTC ATTTGAAGGA 
GAGATGCTGA TGATTGACAA CCTCTCTGGC TCCGTCTTCC ATCATTACTC CTCTCCTCCT 
GTCTGCAAGG ACTGCAGGAC TCATCCGCAA GAGCAGATCC ACATGGCTGA ACTGGAGCAG 
GCGTCTCTGA GGTCGGAGCA GCTCTCAGAT AAGTCATCCT CGCCAGCGCT GCCCGATGAC 
CTCAGCCTGG ACGAGCAGAG CGTCTACCAG ATGGCTGCCA AAGAGGGCAA GGAGAGAGTC 
TGTGATGAGT GCCATCATTC AGAAGAGCCA GGAGGGAGAG GAAACAGCAG GTTACGACGA 
TCCTCTCGCG TCCACAGCTC TGGGACAGAA GATGGAGGAA CTTGTCAAAG TCCACATCAC 
AACACACCTG CTCGACACAG CATAGGTGGC GTTCCCTGTA GCAGCAGTAG TCAAGAGCAC 
CCAGGGGTTT CTGGAGGTTC GAGCCGCTCT AACACCCCCG TTTGTCTCCC TGCTGAATTC 
TTCCACCCAG CTGCTGCGGC TCTGCCTGCA CCTCCGAGAG GCCGAACAGG ACACAAACCA 
AGAGGCCTTC GGCTAACTTC ACCAGCCTCT TGGGCTTCTC TGCGCTCTCC TGGAGCAAAC 
ACCAGGCTGC TCACCACACA GTACCCATCT CACAGTGACT CCTCTCTGGC TACGGGCAGC 
TCAGAGGGCA GCCTACCGAC CACCATGGAA GAAGGGCTCA GTTTCATCGT ATCAACGCCC 
CAGCCCCTTC TGGACACACT CCTCGACGAG CGTACTAGCC TCTCCACAGA GACCCTGAGA 
CCCAGTCCTC CAGCCACACA CATCCTCCAA ACCACTAGGG GGCACCAGCG CAGCAGGAGC 
AGCTGTGAAA CGGACATCAA TACGGGCAGC ATTCGACAAG GTTCCGAGGC GGATGTAGGA 
TTGGTATGGT GCAGCTCCGA GCAGACATGC GACAGCCAGC AGCTCTCAGA GACGCAGAGC 
AGCCTGTCGC TCACTTCGCT ACTTCTACCA AGCTCTCTAG TGCCCCTGTC TGTGAAAAAG 
TGCAACAGCA CGGGCAGCTT AGACCAGGGG ACTCTCACAG CCCAAGAAAA AGTGTTCACG 
AAAGAGCCAC AGGGTAATAT CACCAGCCCC TGGAAAGAGA GGAAGGAGAG GCAGTCGGAG 
GCAGCACAAG GAACAGATGG GGAAAGTACA AGCCAAATAA CAATTGCGGG CTCTAGGAAA 
AATAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1i ( XM_021473957.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329632.1 Danio rerio calcium channel, voltage-dependent, T type, alpha 1I subunit (cacna1i), transcript variant X2, mRNA.

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CloneID	ODa141760
Clone ID Related Accession (Same CDS sequence)	XM_021473957.1
Accession Version	XM_021473957.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6366bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent T-type calcium channel subunit alpha-1I isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGTTG GCCTTAACTC TTGCTTCCTC ACGTGGTTCG AGCGCATCAG TATAATGGTG 
ATCCTGCTGA ACTGCGTCAC TCTGGGGATG TACCAGCCTT GCGAGAACAT CGACTGCACT 
TCAGAGCGCT GTCAGGTCCT ACAGGCTTTT GATGCATTTA TCTACATTTT CTTTGCACTT 
GAGATGGTGG TGAAGATGGT GGCTCTGGGG ATCTTTGGCC GTCGCTGCTA TCTTGGCGAT 
ACTTGGAACA GGCTGGACTT TTTCATCGTG ATGGCAGGGA TGGTGGAATA CTCCCTTGAT 
CTCCAGAACA TCAACTTCTC CGCCATCAGA ACAGTGCGTG TGCTAAGACC TCTCAAAGCC 
ATCAACAGAG TACCCAGTAT GCGCATCCTG GTGAACCTGC TGCTGGACAC TCTGCCCATG 
CTGGGAAATG TGCTCCTGCT TTGTTTCTTT GTGTTCTTCA TTTTTGGCAT CATTGGTGTA 
CAACTGTGGG CTGGACTCCT AAGAAATCGG TGTTACCCAG AGGAGAACTT CACCCTCACA 
TCTGGCCTCA CCCTTCCCAC ACCATATTAT CAGCCTGAGG AGGATGACGA GCGGCCCTTC 
ATCTGCTCGT TGGCTCAAGA TAACGGCATC ATGTCCTGCA GCGATGTGCC TGCTCGGAGA 
GAGGGCGGAC GGGAGTGCTG TCTGGATAAA GAGGACGCTC TTCACCGGCA GGCTCTGGGT 
CTGAGCGCCG AGCCGCTGGT CAACGGCAGC GCATCTGCCA TGGGCCTCTG TGTGAACTGG 
AATCAGTACT ACACCCGCTG CCACACTGGA CACACCAATC CTCATAAAGG AGCAATTAAT 
TTCGACAACA TAGGATACGC ATGGATCGTC ATATTTCAGG TGATCACGTT AGAGGGCTGG 
GTGGAAATCA TGTACTACGT CATGGACGCG CATTCCTTCT ACAACTTCAT CTATTTTATA 
TTCCTCATCA TCATTGGATC TTTCTTCATG ATTAATCTGT GCCTGGTGGT CATTGCCACT 
CAGTTTTCGG AGACCAAACA GCGAGAGCAC CAGCTGATGC AAGAGCAACG TGCTCGCTAC 
CTGTCCTCCA GCACGCTGGC TTCACTCGCT GAGCCCGGAG ACTGTTACGA GGAGCTCTTC 
CAACTGGTCT GCCATATCCT ACGCAAAGCC CGCCGCCGCT CTGCTGCCCT TTATTACATG 
CTCCGTGGCA AAGCCCCTCC CCCTGGAGGT GGGAGGGGGC GTGGCAAAGG AGGAGCAGGA 
TCGATAGGAG GAGGAGCCAA TGTTAATGGA GGAGAGAAAC ATCACTGCCA TTCAGCTCAG 
ACATCTCACT GTATTCACGA AACCAAATTG GACCACAGCT CAGAGAATGC AGTCTCCCTC 
TCCATCAGTC CAAACCCAGA GGAGTGTCCA CAGTGTGCTG CAGCCCTGTC TCTCAAAGAG 
GGGGAGGAGT CAACAGGGCA TTCAGCCAAT GGGGAGGAAG AGGAGGGTGC GCTGGAGGAA 
ACTGACAGGG ACGAGAACCG CTTAGACAAA AAGAGGAGCA GCGACTCTGA TGACACGCTA 
AAGAAGAAAC GCACCTGTTT GGGGAAATGT AAAGACGTCT GGGACGAAAT AAGAGTGAAA 
CTTTGGGGCA TCGTTGAAAG CAAGTACTTC AACAGAGGGA TTATGATTGC CATCCTCATC 
AATACAATCA GCATGGGCAT CGAGCACCAT AACCAGCCTG ATGAACTGAC TAATGTCCTG 
GAGATCTGTA ACATAGTGTT CACCAGTATG TTCACTCTGG AGATGATCCT CAAGCTCACT 
GCCTTTGGCT TCTTTGAATA TCTGAGAAAC CCTTACAACA TCTTTGACGG CATCATTGTG 
ATCATCAGTG TATGTGAGAT CATTGGTCAG TCTGACGGAG GTCTGTCAGT CCTGAGGACG 
TTCAGATTGC TGAGGGTGAT AAAGCTGGTG CGGTTCATGC CAGCTCTGCG CAGGCAGCTG 
GTGGTCCTGA TGAAGACCAT GGACAATGTG GCCACGTTCT GCATGCTGCT AATGCTCTTC 
ATCTTTATAT TCAGTATTCT GGGAATGCAC ATATTTGGCT GCAAGTTCAG CCTGAAGACA 
GAGGCTGGGG ACACCGTACC TGACAGGAAG AACTTTGACT CTCTGCTTTG GGCCATTGTT 
ACAGTGTTTC AGATCTTAAC TCAAGAGGAC TGGAACATGG TTTTGTATAA CGGCATGGCC 
TCCACCTCGC CTCTGGCAGC CCTGTATTTC GTCGCGCTCA TGACTTTTGG AAACTACGTT 
CTATTCAATC TGCTTGTGGC CATCTTGGTT GAAGGATTTC AGGCTGAGGG CGATGCTAAC 
CGTTCCTACT CGGATGATGA CAGATCATCT TGTAATTTAG AAGAAACCGA GAAAAAGGAC 
TCTCTCCAGC TGTCAGATCC CAAGATTTCC ACGTTGACCC CTAACGGGCA TCTGGATTTA 
GCACCTGCAC CCAATGCTAG GGGCTTTTAC CCATCTGAAA GGCTGAGCTT TGCCCTGGGT 
TCGCGCAAGA GCAGCGTTAG CTCTCTGGGC AGGGCTAGCC TGGAGCAAAC CACGCTGTGT 
CCAGGACGAG CATCTCTTTA CCACAATTGG GGACGCCCCG CACGGCCAGG AATATGGAGC 
CGCAGGTCGA GTTGGAACAG CTTGGGACGG TCCTCCAGAT GTCTGGGGAT GGGGGGTGGA 
GGCAGTTTAC GAGTCCGTAG CCCTCACTGT CATCCCGCCG AGCAGGAATC CCTGCTGTCT 
CCTCCACCTC CGCCTCTCCA TCCGCCTCTG CTCCCCAGAC ACTTTCCCCC ACGCAGGGAA 
CGCAGGGCTC TGTCTCTGGA GCTCCCAGAG CTTCTACAGG TGCCAGGACC ACCCCTGCCT 
CCTCTGCACC CCCGCCAGCG CAAGAAGAGC TTCTCTGGGG GTCTGGGAAG CGTTGGAGAG 
CATCAGGACT GTAATGGGAA AACGCCATCA GTTCAACCAC AAATCATTAA TGAAGTCTAC 
CCTCAGGTCA ACACACGCAA GGACAGGGAG GATCTAGAGG ATGAGCTAGA CTATAGTCTG 
TGTTTCCGGA TCCAGAAGAT GATGGAGGTG TACAGACCGG ACTGGTGTGA GACCAGGGAA 
GACTGGTCTG TCTTCCTCTT CTCTCCTCAA AACAAGTTCA GGTTGTTGTG TCAGTCCATC 
ATAGCCCACA AGCTCTTTGA CTATGTGGTG CTGGCCTTCA TCTTCTCAAA CTGCATCACT 
GTGGCTCTGG AGAGACCCAA GATCCTTCAG GGTAGCCTGG AGAGGCTTTT CCTTACCGTG 
TCCAATTACA TTTTCACAGC CATATTTGTG GGGGAGATGA CGCTCAAGGT GGTGTCTATG 
GGTTTGTATA TTGGAGAGCA GGCATACTTG CGCAGCAGCT GGAATATTTT GGATGGTTTC 
CTGGTGTTTG TGTCTTTGAT TGATATTGTG GTGTCGATGG CAGGCGGGGC TAAGATCCTG 
GGGGTGCTCA GGGTCCTCAG GCTTCTTCGC ACACTGCGCC CTCTCAGGGT TATCAGTCGA 
GCTCCTGGTC TCAAACTAGT TGTGGAGACT TTGATCACCT CTCTTAAGCC TATTGGAAAC 
ATAGTTCTCA TCTGCTGTGC CTTTTTCATT ATTTTTGGGA TCCTGGGAGT TCAGCTTTTC 
AAAGGCAAGT TCTACTACTG CTTGGGCCTG GATGTCAAAA ACATCACCAA CAAGTCTGAC 
TGCCTCTTGG CCAATTATAA GTGGGTCCAT CACAAGTACA ACTTTGACAA CTTGGGGCAG 
GCTCTGATGT CCTTGTTTGT ACTGGCTTCG AAGGACGGCT GGGTCAACAT CATGTATCAT 
GGTTTGGATG CTGTGGCAGT GGACCAACAG CCCATAACCA ACAACAACCC CTGGATGCTG 
CTGTACTTCA TCTCATTCCT CTTGATCGTC AGTTTCTTCG TGCTGAATAT GTTTGTTGGA 
GTGGTGGTGG AGAACTTCCA TAAATGCCGA CAGCATCAGG AGGTGGAGGA GGCCAAGAGG 
AGGGAGGAGA AGAGACAGAG ACGCATGGAG AAAAAGAGAC GGAAGGCCCA GAAGTTGCCC 
TACTATGCCA GTTATAGTCA TGTACGGCTG ATGATCCACA CCCTGTGCAC CAGTCACTAC 
CTGGATATCT TCATCACCTT CATCATCTGT GTGAATGTAG TCACCATGTC ACTGGAGCAC 
TACAGCCAGC CTCATTCTCT GGAGATCGCA CTGAAGTACT GTAATTATTT CTTCACCAGC 
ACCTTTGTGC TGGAGGCTGT CCTGAAGCTC ATCGCATTTG GCTTCCGACG TTTCTTCAAA 
GACAGGTGGA ACCAGCTGGA TTTGGCAATT GTACTGCTGT CTGTCATGGG CATCACGCTG 
GAGGAGATTG AAATCAGTGC AGCTCTGCCC ATCAACCCCA CCATAATACG TATCATGAGG 
GTCCTACGCA TCGCACGTGT GTTAAAGCTG TTAAAGATGG CCACAGGAAT GAGAGCGCTG 
CTGGATACAG TAGTTCAAGC CCTGCCACAG GTGGGGAACC TTGGCCTCCT CTTCATGCTG 
CTGTTCTTCA TCTATGCTGC ACTGGGGGTG GAATTGTTCG GAGAGCTGGT GTGCAATGAA 
GATTACCCAT GTGAAGGCAT GAGTCGTCAT GCCACCTTTG AGAACTTCGG CATGGCGTTC 
CTCACCCTTT TCCAGGTCTC CACTGGCGAT AACTGGAATG GCATAATGAA GGACACTCTC 
CGTGAATGTC CGCCAGGCGA ATACACCTGT AATCCCAGCC TACAATTCAT CTCCCCGCTG 
TACTTTGTGA GCTTTGTGCT CACGGCCCAG TTTGTCCTCA TCAATGTGGT GGTGGCGGTG 
CTGATGAAAC ACCTGGACGA CTCAAACAAG GAGGCCCAGG AGGAGGCAGA GATGGATGCC 
GAGATTGAAA TGGAGCTGGC CCAGGGAACG CTCTGCTGCA TCGGCGGAGG GGTAGCCGTA 
GCCGACAGGA CCGGCATGGG GCATCAGGGG GCTGCATCCT GCTCCGTTGT TCCCCCTCAC 
TCTCCAATCA CACACCCAGT GGCCCACGAA CCCAACAGCA TCAGGAGACT CTACTCACCG 
GCGCAGGAGA ACTTGTGGCT GGACAGTGTA TCTCTGCTCA TCAAAGATTC ATTTGAAGGA 
GAGATGCTGA TGATTGACAA CCTCTCTGGC TCCGTCTTCC ATCATTACTC CTCTCCTCCT 
GTCTGCAAGG ACTGCAGGAC TCATCCGCAA GAGATCCACA TGGCTGAACT GGAGCAGGCG 
TCTCTGAGGT CGGAGCAGCT CTCAGATAAG TCATCCTCGC CAGCGCTGCC CGATGACCTC 
AGCCTGGACG AGCAGAGCGT CTACCAGATG GCTGCCAAAG AGGGCAAGGA GAGAGTCTGT 
GATGAGTGCC ATCATTCAGA AGAGCCAGGA GGGAGAGGAA ACAGCAGGTT ACGACGATCC 
TCTCGCGTCC ACAGCTCTGG GACAGAAGAT GGAGGAACTT GTCAAAGTCC ACATCACAAC 
ACACCTGCTC GACACAGCAT AGGTGGCGTT CCCTGTAGCA GCAGTAGTCA AGAGCACCCA 
GGGGTTTCTG GAGGTTCGAG CCGCTCTAAC ACCCCCGTTT GTCTCCCTGC TGAATTCTTC 
CACCCAGCTG CTGCGGCTCT GCCTGCACCT CCGAGAGGCC GAACAGGACA CAAACCAAGA 
GGCCTTCGGC TAACTTCACC AGCCTCTTGG GCTTCTCTGC GCTCTCCTGG AGCAAACACC 
AGGCTGCTCA CCACACAGTA CCCATCTCAC AGTGACTCCT CTCTGGCTAC GGGCAGCTCA 
GAGGGCAGCC TACCGACCAC CATGGAAGAA GGGCTCAGTT TCATCGTATC AACGCCCCAG 
CCCCTTCTGG ACACACTCCT CGACGAGCGT ACTAGCCTCT CCACAGAGAC CCTGAGACCC 
AGTCCTCCAG CCACACACAT CCTCCAAACC ACTAGGGGGC ACCAGCGCAG CAGGAGCAGC 
TGTGAAACGG ACATCAATAC GGGCAGCATT CGACAAGGTT CCGAGGCGGA TGTAGGATTG 
GTATGGTGCA GCTCCGAGCA GACATGCGAC AGCCAGCAGC TCTCAGAGAC GCAGAGCAGC 
CTGTCGCTCA CTTCGCTACT TCTACCAAGC TCTCTAGTGC CCCTGTCTGT GAAAAAGTGC 
AACAGCACGG GCAGCTTAGA CCAGGGGACT CTCACAGCCC AAGAAAAAGT GTTCACGAAA 
GAGCCACAGG GTAATATCAC CAGCCCCTGG AAAGAGAGGA AGGAGAGGCA GTCGGAGGCA 
GCACAAGGAA CAGATGGGGA AAGTACAAGC CAAATAACAA TTGCGGGCTC TAGGAAAAAT 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329632.1
CDS	1300..7665
Translation	MNVGLNSCFLTWFERISIMVILLNCVTLGMYQPCENIDCTSERCQVLQAFDAFIYIFFALEMVVKMVALGIFGRRCYLGDTWNRLDFFIVMAGMVEYSLDLQNINFSAIRTVRVLRPLKAINRVPSMRILVNLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWAGLLRNRCYPEENFTLTSGLTLPTPYYQPEEDDERPFICSLAQDNGIMSCSDVPARREGGRECCLDKEDALHRQALGLSAEPLVNGSASAMGLCVNWNQYYTRCHTGHTNPHKGAINFDNIGYAWIVIFQVITLEGWVEIMYYVMDAHSFYNFIYFIFLIIIGSFFMINLCLVVIATQFSETKQREHQLMQEQRARYLSSSTLASLAEPGDCYEELFQLVCHILRKARRRSAALYYMLRGKAPPPGGGRGRGKGGAGSIGGGANVNGGEKHHCHSAQTSHCIHETKLDHSSENAVSLSISPNPEECPQCAAALSLKEGEESTGHSANGEEEEGALEETDRDENRLDKKRSSDSDDTLKKKRTCLGKCKDVWDEIRVKLWGIVESKYFNRGIMIAILINTISMGIEHHNQPDELTNVLEICNIVFTSMFTLEMILKLTAFGFFEYLRNPYNIFDGIIVIISVCEIIGQSDGGLSVLRTFRLLRVIKLVRFMPALRRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHIFGCKFSLKTEAGDTVPDRKNFDSLLWAIVTVFQILTQEDWNMVLYNGMASTSPLAALYFVALMTFGNYVLFNLLVAILVEGFQAEGDANRSYSDDDRSSCNLEETEKKDSLQLSDPKISTLTPNGHLDLAPAPNARGFYPSERLSFALGSRKSSVSSLGRASLEQTTLCPGRASLYHNWGRPARPGIWSRRSSWNSLGRSSRCLGMGGGGSLRVRSPHCHPAEQESLLSPPPPPLHPPLLPRHFPPRRERRALSLELPELLQVPGPPLPPLHPRQRKKSFSGGLGSVGEHQDCNGKTPSVQPQIINEVYPQVNTRKDREDLEDELDYSLCFRIQKMMEVYRPDWCETREDWSVFLFSPQNKFRLLCQSIIAHKLFDYVVLAFIFSNCITVALERPKILQGSLERLFLTVSNYIFTAIFVGEMTLKVVSMGLYIGEQAYLRSSWNILDGFLVFVSLIDIVVSMAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLITSLKPIGNIVLICCAFFIIFGILGVQLFKGKFYYCLGLDVKNITNKSDCLLANYKWVHHKYNFDNLGQALMSLFVLASKDGWVNIMYHGLDAVAVDQQPITNNNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEVEEAKRREEKRQRRMEKKRRKAQKLPYYASYSHVRLMIHTLCTSHYLDIFITFIICVNVVTMSLEHYSQPHSLEIALKYCNYFFTSTFVLEAVLKLIAFGFRRFFKDRWNQLDLAIVLLSVMGITLEEIEISAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGELVCNEDYPCEGMSRHATFENFGMAFLTLFQVSTGDNWNGIMKDTLRECPPGEYTCNPSLQFISPLYFVSFVLTAQFVLINVVVAVLMKHLDDSNKEAQEEAEMDAEIEMELAQGTLCCIGGGVAVADRTGMGHQGAASCSVVPPHSPITHPVAHEPNSIRRLYSPAQENLWLDSVSLLIKDSFEGEMLMIDNLSGSVFHHYSSPPVCKDCRTHPQEIHMAELEQASLRSEQLSDKSSSPALPDDLSLDEQSVYQMAAKEGKERVCDECHHSEEPGGRGNSRLRRSSRVHSSGTEDGGTCQSPHHNTPARHSIGGVPCSSSSQEHPGVSGGSSRSNTPVCLPAEFFHPAAAALPAPPRGRTGHKPRGLRLTSPASWASLRSPGANTRLLTTQYPSHSDSSLATGSSEGSLPTTMEEGLSFIVSTPQPLLDTLLDERTSLSTETLRPSPPATHILQTTRGHQRSRSSCETDINTGSIRQGSEADVGLVWCSSEQTCDSQQLSETQSSLSLTSLLLPSSLVPLSVKKCNSTGSLDQGTLTAQEKVFTKEPQGNITSPWKERKERQSEAAQGTDGESTSQITIAGSRKNR

Target ORF information:

RefSeq Version	XM_021473957.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, T type, alpha 1I subunit (cacna1i), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021473957.1

ORF Insert Sequence:

ATGAATGTTG GCCTTAACTC TTGCTTCCTC ACGTGGTTCG AGCGCATCAG TATAATGGTG 
ATCCTGCTGA ACTGCGTCAC TCTGGGGATG TACCAGCCTT GCGAGAACAT CGACTGCACT 
TCAGAGCGCT GTCAGGTCCT ACAGGCTTTT GATGCATTTA TCTACATTTT CTTTGCACTT 
GAGATGGTGG TGAAGATGGT GGCTCTGGGG ATCTTTGGCC GTCGCTGCTA TCTTGGCGAT 
ACTTGGAACA GGCTGGACTT TTTCATCGTG ATGGCAGGGA TGGTGGAATA CTCCCTTGAT 
CTCCAGAACA TCAACTTCTC CGCCATCAGA ACAGTGCGTG TGCTAAGACC TCTCAAAGCC 
ATCAACAGAG TACCCAGTAT GCGCATCCTG GTGAACCTGC TGCTGGACAC TCTGCCCATG 
CTGGGAAATG TGCTCCTGCT TTGTTTCTTT GTGTTCTTCA TTTTTGGCAT CATTGGTGTA 
CAACTGTGGG CTGGACTCCT AAGAAATCGG TGTTACCCAG AGGAGAACTT CACCCTCACA 
TCTGGCCTCA CCCTTCCCAC ACCATATTAT CAGCCTGAGG AGGATGACGA GCGGCCCTTC 
ATCTGCTCGT TGGCTCAAGA TAACGGCATC ATGTCCTGCA GCGATGTGCC TGCTCGGAGA 
GAGGGCGGAC GGGAGTGCTG TCTGGATAAA GAGGACGCTC TTCACCGGCA GGCTCTGGGT 
CTGAGCGCCG AGCCGCTGGT CAACGGCAGC GCATCTGCCA TGGGCCTCTG TGTGAACTGG 
AATCAGTACT ACACCCGCTG CCACACTGGA CACACCAATC CTCATAAAGG AGCAATTAAT 
TTCGACAACA TAGGATACGC ATGGATCGTC ATATTTCAGG TGATCACGTT AGAGGGCTGG 
GTGGAAATCA TGTACTACGT CATGGACGCG CATTCCTTCT ACAACTTCAT CTATTTTATA 
TTCCTCATCA TCATTGGATC TTTCTTCATG ATTAATCTGT GCCTGGTGGT CATTGCCACT 
CAGTTTTCGG AGACCAAACA GCGAGAGCAC CAGCTGATGC AAGAGCAACG TGCTCGCTAC 
CTGTCCTCCA GCACGCTGGC TTCACTCGCT GAGCCCGGAG ACTGTTACGA GGAGCTCTTC 
CAACTGGTCT GCCATATCCT ACGCAAAGCC CGCCGCCGCT CTGCTGCCCT TTATTACATG 
CTCCGTGGCA AAGCCCCTCC CCCTGGAGGT GGGAGGGGGC GTGGCAAAGG AGGAGCAGGA 
TCGATAGGAG GAGGAGCCAA TGTTAATGGA GGAGAGAAAC ATCACTGCCA TTCAGCTCAG 
ACATCTCACT GTATTCACGA AACCAAATTG GACCACAGCT CAGAGAATGC AGTCTCCCTC 
TCCATCAGTC CAAACCCAGA GGAGTGTCCA CAGTGTGCTG CAGCCCTGTC TCTCAAAGAG 
GGGGAGGAGT CAACAGGGCA TTCAGCCAAT GGGGAGGAAG AGGAGGGTGC GCTGGAGGAA 
ACTGACAGGG ACGAGAACCG CTTAGACAAA AAGAGGAGCA GCGACTCTGA TGACACGCTA 
AAGAAGAAAC GCACCTGTTT GGGGAAATGT AAAGACGTCT GGGACGAAAT AAGAGTGAAA 
CTTTGGGGCA TCGTTGAAAG CAAGTACTTC AACAGAGGGA TTATGATTGC CATCCTCATC 
AATACAATCA GCATGGGCAT CGAGCACCAT AACCAGCCTG ATGAACTGAC TAATGTCCTG 
GAGATCTGTA ACATAGTGTT CACCAGTATG TTCACTCTGG AGATGATCCT CAAGCTCACT 
GCCTTTGGCT TCTTTGAATA TCTGAGAAAC CCTTACAACA TCTTTGACGG CATCATTGTG 
ATCATCAGTG TATGTGAGAT CATTGGTCAG TCTGACGGAG GTCTGTCAGT CCTGAGGACG 
TTCAGATTGC TGAGGGTGAT AAAGCTGGTG CGGTTCATGC CAGCTCTGCG CAGGCAGCTG 
GTGGTCCTGA TGAAGACCAT GGACAATGTG GCCACGTTCT GCATGCTGCT AATGCTCTTC 
ATCTTTATAT TCAGTATTCT GGGAATGCAC ATATTTGGCT GCAAGTTCAG CCTGAAGACA 
GAGGCTGGGG ACACCGTACC TGACAGGAAG AACTTTGACT CTCTGCTTTG GGCCATTGTT 
ACAGTGTTTC AGATCTTAAC TCAAGAGGAC TGGAACATGG TTTTGTATAA CGGCATGGCC 
TCCACCTCGC CTCTGGCAGC CCTGTATTTC GTCGCGCTCA TGACTTTTGG AAACTACGTT 
CTATTCAATC TGCTTGTGGC CATCTTGGTT GAAGGATTTC AGGCTGAGGG CGATGCTAAC 
CGTTCCTACT CGGATGATGA CAGATCATCT TGTAATTTAG AAGAAACCGA GAAAAAGGAC 
TCTCTCCAGC TGTCAGATCC CAAGATTTCC ACGTTGACCC CTAACGGGCA TCTGGATTTA 
GCACCTGCAC CCAATGCTAG GGGCTTTTAC CCATCTGAAA GGCTGAGCTT TGCCCTGGGT 
TCGCGCAAGA GCAGCGTTAG CTCTCTGGGC AGGGCTAGCC TGGAGCAAAC CACGCTGTGT 
CCAGGACGAG CATCTCTTTA CCACAATTGG GGACGCCCCG CACGGCCAGG AATATGGAGC 
CGCAGGTCGA GTTGGAACAG CTTGGGACGG TCCTCCAGAT GTCTGGGGAT GGGGGGTGGA 
GGCAGTTTAC GAGTCCGTAG CCCTCACTGT CATCCCGCCG AGCAGGAATC CCTGCTGTCT 
CCTCCACCTC CGCCTCTCCA TCCGCCTCTG CTCCCCAGAC ACTTTCCCCC ACGCAGGGAA 
CGCAGGGCTC TGTCTCTGGA GCTCCCAGAG CTTCTACAGG TGCCAGGACC ACCCCTGCCT 
CCTCTGCACC CCCGCCAGCG CAAGAAGAGC TTCTCTGGGG GTCTGGGAAG CGTTGGAGAG 
CATCAGGACT GTAATGGGAA AACGCCATCA GTTCAACCAC AAATCATTAA TGAAGTCTAC 
CCTCAGGTCA ACACACGCAA GGACAGGGAG GATCTAGAGG ATGAGCTAGA CTATAGTCTG 
TGTTTCCGGA TCCAGAAGAT GATGGAGGTG TACAGACCGG ACTGGTGTGA GACCAGGGAA 
GACTGGTCTG TCTTCCTCTT CTCTCCTCAA AACAAGTTCA GGTTGTTGTG TCAGTCCATC 
ATAGCCCACA AGCTCTTTGA CTATGTGGTG CTGGCCTTCA TCTTCTCAAA CTGCATCACT 
GTGGCTCTGG AGAGACCCAA GATCCTTCAG GGTAGCCTGG AGAGGCTTTT CCTTACCGTG 
TCCAATTACA TTTTCACAGC CATATTTGTG GGGGAGATGA CGCTCAAGGT GGTGTCTATG 
GGTTTGTATA TTGGAGAGCA GGCATACTTG CGCAGCAGCT GGAATATTTT GGATGGTTTC 
CTGGTGTTTG TGTCTTTGAT TGATATTGTG GTGTCGATGG CAGGCGGGGC TAAGATCCTG 
GGGGTGCTCA GGGTCCTCAG GCTTCTTCGC ACACTGCGCC CTCTCAGGGT TATCAGTCGA 
GCTCCTGGTC TCAAACTAGT TGTGGAGACT TTGATCACCT CTCTTAAGCC TATTGGAAAC 
ATAGTTCTCA TCTGCTGTGC CTTTTTCATT ATTTTTGGGA TCCTGGGAGT TCAGCTTTTC 
AAAGGCAAGT TCTACTACTG CTTGGGCCTG GATGTCAAAA ACATCACCAA CAAGTCTGAC 
TGCCTCTTGG CCAATTATAA GTGGGTCCAT CACAAGTACA ACTTTGACAA CTTGGGGCAG 
GCTCTGATGT CCTTGTTTGT ACTGGCTTCG AAGGACGGCT GGGTCAACAT CATGTATCAT 
GGTTTGGATG CTGTGGCAGT GGACCAACAG CCCATAACCA ACAACAACCC CTGGATGCTG 
CTGTACTTCA TCTCATTCCT CTTGATCGTC AGTTTCTTCG TGCTGAATAT GTTTGTTGGA 
GTGGTGGTGG AGAACTTCCA TAAATGCCGA CAGCATCAGG AGGTGGAGGA GGCCAAGAGG 
AGGGAGGAGA AGAGACAGAG ACGCATGGAG AAAAAGAGAC GGAAGGCCCA GAAGTTGCCC 
TACTATGCCA GTTATAGTCA TGTACGGCTG ATGATCCACA CCCTGTGCAC CAGTCACTAC 
CTGGATATCT TCATCACCTT CATCATCTGT GTGAATGTAG TCACCATGTC ACTGGAGCAC 
TACAGCCAGC CTCATTCTCT GGAGATCGCA CTGAAGTACT GTAATTATTT CTTCACCAGC 
ACCTTTGTGC TGGAGGCTGT CCTGAAGCTC ATCGCATTTG GCTTCCGACG TTTCTTCAAA 
GACAGGTGGA ACCAGCTGGA TTTGGCAATT GTACTGCTGT CTGTCATGGG CATCACGCTG 
GAGGAGATTG AAATCAGTGC AGCTCTGCCC ATCAACCCCA CCATAATACG TATCATGAGG 
GTCCTACGCA TCGCACGTGT GTTAAAGCTG TTAAAGATGG CCACAGGAAT GAGAGCGCTG 
CTGGATACAG TAGTTCAAGC CCTGCCACAG GTGGGGAACC TTGGCCTCCT CTTCATGCTG 
CTGTTCTTCA TCTATGCTGC ACTGGGGGTG GAATTGTTCG GAGAGCTGGT GTGCAATGAA 
GATTACCCAT GTGAAGGCAT GAGTCGTCAT GCCACCTTTG AGAACTTCGG CATGGCGTTC 
CTCACCCTTT TCCAGGTCTC CACTGGCGAT AACTGGAATG GCATAATGAA GGACACTCTC 
CGTGAATGTC CGCCAGGCGA ATACACCTGT AATCCCAGCC TACAATTCAT CTCCCCGCTG 
TACTTTGTGA GCTTTGTGCT CACGGCCCAG TTTGTCCTCA TCAATGTGGT GGTGGCGGTG 
CTGATGAAAC ACCTGGACGA CTCAAACAAG GAGGCCCAGG AGGAGGCAGA GATGGATGCC 
GAGATTGAAA TGGAGCTGGC CCAGGGAACG CTCTGCTGCA TCGGCGGAGG GGTAGCCGTA 
GCCGACAGGA CCGGCATGGG GCATCAGGGG GCTGCATCCT GCTCCGTTGT TCCCCCTCAC 
TCTCCAATCA CACACCCAGT GGCCCACGAA CCCAACAGCA TCAGGAGACT CTACTCACCG 
GCGCAGGAGA ACTTGTGGCT GGACAGTGTA TCTCTGCTCA TCAAAGATTC ATTTGAAGGA 
GAGATGCTGA TGATTGACAA CCTCTCTGGC TCCGTCTTCC ATCATTACTC CTCTCCTCCT 
GTCTGCAAGG ACTGCAGGAC TCATCCGCAA GAGATCCACA TGGCTGAACT GGAGCAGGCG 
TCTCTGAGGT CGGAGCAGCT CTCAGATAAG TCATCCTCGC CAGCGCTGCC CGATGACCTC 
AGCCTGGACG AGCAGAGCGT CTACCAGATG GCTGCCAAAG AGGGCAAGGA GAGAGTCTGT 
GATGAGTGCC ATCATTCAGA AGAGCCAGGA GGGAGAGGAA ACAGCAGGTT ACGACGATCC 
TCTCGCGTCC ACAGCTCTGG GACAGAAGAT GGAGGAACTT GTCAAAGTCC ACATCACAAC 
ACACCTGCTC GACACAGCAT AGGTGGCGTT CCCTGTAGCA GCAGTAGTCA AGAGCACCCA 
GGGGTTTCTG GAGGTTCGAG CCGCTCTAAC ACCCCCGTTT GTCTCCCTGC TGAATTCTTC 
CACCCAGCTG CTGCGGCTCT GCCTGCACCT CCGAGAGGCC GAACAGGACA CAAACCAAGA 
GGCCTTCGGC TAACTTCACC AGCCTCTTGG GCTTCTCTGC GCTCTCCTGG AGCAAACACC 
AGGCTGCTCA CCACACAGTA CCCATCTCAC AGTGACTCCT CTCTGGCTAC GGGCAGCTCA 
GAGGGCAGCC TACCGACCAC CATGGAAGAA GGGCTCAGTT TCATCGTATC AACGCCCCAG 
CCCCTTCTGG ACACACTCCT CGACGAGCGT ACTAGCCTCT CCACAGAGAC CCTGAGACCC 
AGTCCTCCAG CCACACACAT CCTCCAAACC ACTAGGGGGC ACCAGCGCAG CAGGAGCAGC 
TGTGAAACGG ACATCAATAC GGGCAGCATT CGACAAGGTT CCGAGGCGGA TGTAGGATTG 
GTATGGTGCA GCTCCGAGCA GACATGCGAC AGCCAGCAGC TCTCAGAGAC GCAGAGCAGC 
CTGTCGCTCA CTTCGCTACT TCTACCAAGC TCTCTAGTGC CCCTGTCTGT GAAAAAGTGC 
AACAGCACGG GCAGCTTAGA CCAGGGGACT CTCACAGCCC AAGAAAAAGT GTTCACGAAA 
GAGCCACAGG GTAATATCAC CAGCCCCTGG AAAGAGAGGA AGGAGAGGCA GTCGGAGGCA 
GCACAAGGAA CAGATGGGGA AAGTACAAGC CAAATAACAA TTGCGGGCTC TAGGAAAAAT 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1i ( XM_009299474.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009297749.1 Danio rerio calcium channel, voltage-dependent, T type, alpha 1I subunit (cacna1i), mRNA.

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CloneID	ODa44854
Clone ID Related Accession (Same CDS sequence)	XM_009299474.2
Accession Version	XM_009299474.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6315bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	voltage-dependent T-type calcium channel subunit alpha-1I
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299474.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGATCC TGCTGAACTG CGTCACTCTG GGGATGTACC AGCCTTGCGA GAACATCGAC 
TGCACTTCAG AGCGCTGTCA GGTCCTACAG GCTTTTGATG CATTTATCTA CATTTTCTTT 
GCACTTGAGA TGGTGGTGAA GATGGTGGCT CTGGGGATCT TTGGCCGTCG CTGCTATCTT 
GGCGATACTT GGAACAGGCT GGACTTTTTC ATCGTGATGG CAGGGATGGT GGAATACTCC 
CTTGATCTCC AGAACATCAA CTTCTCCGCC ATCAGAACAG TGCGTGTGCT AAGACCTCTC 
AAAGCCATCA ACAGAGTACC CAGTATGCGC ATCCTGGTGA ACCTGCTGCT GGACACTCTG 
CCCATGCTGG GAAATGTGCT CCTGCTTTGT TTCTTTGTGT TCTTCATTTT TGGCATCATT 
GGTGTACAAC TGTGGGCTGG ACTCCTAAGA AATCGGTGTT ACCCAGAGGA GAACTTCACC 
CTCACATCTG GCCTCACCCT TCCCACACCA TATTATCAGC CTGAGGAGGA TGACGAGCGG 
CCCTTCATCT GCTCGTTGGC TCAAGATAAC GGCATCATGT CCTGCAGCGA TGTGCCTGCT 
CGGAGAGAGG GCGGACGGGA GTGCTGTCTG GATAAAGAGG ACGCTCTTCA CCGGCAGGCT 
CTGGGTCTGA GCGCCGAGCC GCTGGTCAAC GGCAGCGCAT CTGCCATGGG CCTCTGTGTG 
AACTGGAATC AGTACTACAC CCGCTGCCAC ACTGGACACA CCAATCCTCA TAAAGGAGCA 
ATTAATTTCG ACAACATAGG ATACGCATGG ATCGTCATAT TTCAGGTGAT CACGTTAGAG 
GGCTGGGTGG AAATCATGTA CTACGTCATG GACGCGCATT CCTTCTACAA CTTCATCTAT 
TTTATATTCC TCATCATCAT TGGATCTTTC TTCATGATTA ATCTGTGCCT GGTGGTCATT 
GCCACTCAGT TTTCGGAGAC CAAACAGCGA GAGCACCAGC TGATGCAAGA GCAACGTGCT 
CGCTACCTGT CCTCCAGCAC GCTGGCTTCA CTCGCTGAGC CCGGAGACTG TTACGAGGAG 
CTCTTCCAAC TGGTCTGCCA TATCCTACGC AAAGCCCGCC GCCGCTCTGC TGCCCTTTAT 
TACATGCTCC GTGGCAAAGC CCCTCCCCCT GGAGGTGGGA GGGGGCGTGG CAAAGGAGGA 
GCAGGATCGA TAGGAGGAGG AGCCAATGTT AATGGAGGAG AGAAACATCA CTGCCATTCA 
GCTCAGACAT CTCACTGTAT TCACGAAACC AAATTGGACC ACAGCTCAGA GAATGCAGTC 
TCCCTCTCCA TCAGTCCAAA CCCAGAGGAG TGTCCACAGT GTGCTGCAGC CCTGTCTCTC 
AAAGAGGGGG AGGAGTCAAC AGGGCATTCA GCCAATGGGG AGGAAGAGGA GGGTGCGCTG 
GAGGAAACTG ACAGGGACGA GAACCGCTTA GACAAAAAGA GGAGCAGCGA CTCTGATGAC 
ACGCTAAAGA AGAAACGCAC CTGTTTGGGG AAATGTAAAG ACGTCTGGGA CGAAATAAGA 
GTGAAACTTT GGGGCATCGT TGAAAGCAAG TACTTCAACA GAGGGATTAT GATTGCCATC 
CTCATCAATA CAATCAGCAT GGGCATCGAG CACCATAACC AGCCTGATGA ACTGACTAAT 
GTCCTGGAGA TCTGTAACAT AGTGTTCACC AGTATGTTCA CTCTGGAGAT GATCCTCAAG 
CTCACTGCCT TTGGCTTCTT TGAATATCTG AGAAACCCTT ACAACATCTT TGACGGCATC 
ATTGTGATCA TCAGTGTATG TGAGATCATT GGTCAGTCTG ACGGAGGTCT GTCAGTCCTG 
AGGACGTTCA GATTGCTGAG GGTGATAAAG CTGGTGCGGT TCATGCCAGC TCTGCGCAGG 
CAGCTGGTGG TCCTGATGAA GACCATGGAC AATGTGGCCA CGTTCTGCAT GCTGCTAATG 
CTCTTCATCT TTATATTCAG TATTCTGGGA ATGCACATAT TTGGCTGCAA GTTCAGCCTG 
AAGACAGAGG CTGGGGACAC CGTACCTGAC AGGAAGAACT TTGACTCTCT GCTTTGGGCC 
ATTGTTACAG TGTTTCAGAT CTTAACTCAA GAGGACTGGA ACATGGTTTT GTATAACGGC 
ATGGCCTCCA CCTCGCCTCT GGCAGCCCTG TATTTCGTCG CGCTCATGAC TTTTGGAAAC 
TACGTTCTAT TCAATCTGCT TGTGGCCATC TTGGTTGAAG GATTTCAGGC TGAGGGCGAT 
GCTAACCGTT CCTACTCGGA TGATGACAGA TCATCTTGTA ATTTAGAAGA AACCGAGAAA 
AAGGACTCTC TCCAGCTGTC AGATCCCAAG ATTTCCACGT TGACCCCTAA CGGGCATCTG 
GATTTAGCAC CTGCACCCAA TGCTAGGGGC TTTTACCCAT CTGAAAGGCT GAGCTTTGCC 
CTGGGTTCGC GCAAGAGCAG CGTTAGCTCT CTGGGCAGGG CTAGCCTGGA GCAAACCACG 
CTGTGTCCAG GACGAGCATC TCTTTACCAC AATTGGGGAC GCCCCGCACG GCCAGGAATA 
TGGAGCCGCA GGTCGAGTTG GAACAGCTTG GGACGGTCCT CCAGATGTCT GGGGATGGGG 
GGTGGAGGCA GTTTACGAGT CCGTAGCCCT CACTGTCATC CCGCCGAGCA GGAATCCCTG 
CTGTCTCCTC CACCTCCGCC TCTCCATCCG CCTCTGCTCC CCAGACACTT TCCCCCACGC 
AGGGAACGCA GGGCTCTGTC TCTGGAGCTC CCAGAGCTTC TACAGGTGCC AGGACCACCC 
CTGCCTCCTC TGCACCCCCG CCAGCGCAAG AAGAGCTTCT CTGGGGGTCT GGGAAGCGTT 
GGAGAGCATC AGGACTGTAA TGGGAAAACG CCATCAGTTC AACCACAAAT CATTAATGAA 
GTCTACCCTC AGGTCAACAC ACGCAAGGAC AGGGAGGATC TAGAGGATGA GCTAGACTAT 
AGTCTGTGTT TCCGGATCCA GAAGATGATG GAGGTGTACA GACCGGACTG GTGTGAGACC 
AGGGAAGACT GGTCTGTCTT CCTCTTCTCT CCTCAAAACA AGTTCAGGTT GTTGTGTCAG 
TCCATCATAG CCCACAAGCT CTTTGACTAT GTGGTGCTGG CCTTCATCTT CTCAAACTGC 
ATCACTGTGG CTCTGGAGAG ACCCAAGATC CTTCAGGGTA GCCTGGAGAG GCTTTTCCTT 
ACCGTGTCCA ATTACATTTT CACAGCCATA TTTGTGGGGG AGATGACGCT CAAGGTGGTG 
TCTATGGGTT TGTATATTGG AGAGCAGGCA TACTTGCGCA GCAGCTGGAA TATTTTGGAT 
GGTTTCCTGG TGTTTGTGTC TTTGATTGAT ATTGTGGTGT CGATGGCAGG CGGGGCTAAG 
ATCCTGGGGG TGCTCAGGGT CCTCAGGCTT CTTCGCACAC TGCGCCCTCT CAGGGTTATC 
AGTCGAGCTC CTGGTCTCAA ACTAGTTGTG GAGACTTTGA TCACCTCTCT TAAGCCTATT 
GGAAACATAG TTCTCATCTG CTGTGCCTTT TTCATTATTT TTGGGATCCT GGGAGTTCAG 
CTTTTCAAAG GCAAGTTCTA CTACTGCTTG GGCCTGGATG TCAAAAACAT CACCAACAAG 
TCTGACTGCC TCTTGGCCAA TTATAAGTGG GTCCATCACA AGTACAACTT TGACAACTTG 
GGGCAGGCTC TGATGTCCTT GTTTGTACTG GCTTCGAAGG ACGGCTGGGT CAACATCATG 
TATCATGGTT TGGATGCTGT GGCAGTGGAC CAACAGCCCA TAACCAACAA CAACCCCTGG 
ATGCTGCTGT ACTTCATCTC ATTCCTCTTG ATCGTCAGTT TCTTCGTGCT GAATATGTTT 
GTTGGAGTGG TGGTGGAGAA CTTCCATAAA TGCCGACAGC ATCAGGAGGT GGAGGAGGCC 
AAGAGGAGGG AGGAGAAGAG ACAGAGACGC ATGGAGAAAA AGAGACGGAA GGCCCAGAAG 
TTGCCCTACT ATGCCAGTTA TAGTCATGTA CGGCTGATGA TCCACACCCT GTGCACCAGT 
CACTACCTGG ATATCTTCAT CACCTTCATC ATCTGTGTGA ATGTAGTCAC CATGTCACTG 
GAGCACTACA GCCAGCCTCA TTCTCTGGAG ATCGCACTGA AGTACTGTAA TTATTTCTTC 
ACCAGCACCT TTGTGCTGGA GGCTGTCCTG AAGCTCATCG CATTTGGCTT CCGACGTTTC 
TTCAAAGACA GGTGGAACCA GCTGGATTTG GCAATTGTAC TGCTGTCTGT CATGGGCATC 
ACGCTGGAGG AGATTGAAAT CAGTGCAGCT CTGCCCATCA ACCCCACCAT AATACGTATC 
ATGAGGGTCC TACGCATCGC ACGTGTGTTA AAGCTGTTAA AGATGGCCAC AGGAATGAGA 
GCGCTGCTGG ATACAGTAGT TCAAGCCCTG CCACAGGTGG GGAACCTTGG CCTCCTCTTC 
ATGCTGCTGT TCTTCATCTA TGCTGCACTG GGGGTGGAAT TGTTCGGAGA GCTGGTGTGC 
AATGAAGATT ACCCATGTGA AGGCATGAGT CGTCATGCCA CCTTTGAGAA CTTCGGCATG 
GCGTTCCTCA CCCTTTTCCA GGTCTCCACT GGCGATAACT GGAATGGCAT AATGAAGGAC 
ACTCTCCGTG AATGTCCGCC AGGCGAATAC ACCTGTAATC CCAGCCTACA ATTCATCTCC 
CCGCTGTACT TTGTGAGCTT TGTGCTCACG GCCCAGTTTG TCCTCATCAA TGTGGTGGTG 
GCGGTGCTGA TGAAACACCT GGACGACTCA AACAAGGAGG CCCAGGAGGA GGCAGAGATG 
GATGCCGAGA TTGAAATGGA GCTGGCCCAG GGAACGCTCT GCTGCATCGG CGGAGGGGTA 
GCCGTAGCCG ACAGGACCGG CATGGGGCAT CAGGGGGCTG CATCCTGCTC CGTTGTTCCC 
CCTCACTCTC CAATCACACA CCCAGTGGCC CACGAACCCA ACAGCATCAG GAGACTCTAC 
TCACCGGCGC AGGAGAACTT GTGGCTGGAC AGTGTATCTC TGCTCATCAA AGATTCATTT 
GAAGGAGAGA TGCTGATGAT TGACAACCTC TCTGGCTCCG TCTTCCATCA TTACTCCTCT 
CCTCCTGTCT GCAAGGACTG CAGGACTCAT CCGCAAGAGC AGATCCACAT GGCTGAACTG 
GAGCAGGCGT CTCTGAGGTC GGAGCAGCTC TCAGATAAGT CATCCTCGCC AGCGCTGCCC 
GATGACCTCA GCCTGGACGA GCAGAGCGTC TACCAGATGG CTGCCAAAGA GGGCAAGGAG 
AGAGTCTGTG ATGAGTGCCA TCATTCAGAA GAGCCAGGAG GGAGAGGAAA CAGCAGGTTA 
CGACGATCCT CTCGCGTCCA CAGCTCTGGG ACAGAAGATG GAGGAACTTG TCAAAGTCCA 
CATCACAACA CACCTGCTCG ACACAGCATA GGTGGCGTTC CCTGTAGCAG CAGTAGTCAA 
GAGCACCCAG GGGTTTCTGG AGGTTCGAGC CGCTCTAACA CCCCCGTTTG TCTCCCTGCT 
GAATTCTTCC ACCCAGCTGC TGCGGCTCTG CCTGCACCTC CGAGAGGCCG AACAGGACAC 
AAACCAAGAG GCCTTCGGCT AACTTCACCA GCCTCTTGGG CTTCTCTGCG CTCTCCTGGA 
GCAAACACCA GGCTGCTCAC CACACAGTAC CCATCTCACA GTGACTCCTC TCTGGCTACG 
GGCAGCTCAG AGGGCAGCCT ACCGACCACC ATGGAAGAAG GGCTCAGTTT CATCGTATCA 
ACGCCCCAGC CCCTTCTGGA CACACTCCTC GACGAGCGTA CTAGCCTCTC CACAGAGACC 
CTGAGACCCA GTCCTCCAGC CACACACATC CTCCAAACCA CTAGGGGGCA CCAGCGCAGC 
AGGAGCAGCT GTGAAACGGA CATCAATACG GGCAGCATTC GACAAGGTTC CGAGGCGGAT 
GTAGGATTGG TATGGTGCAG CTCCGAGCAG ACATGCGACA GCCAGCAGCT CTCAGAGACG 
CAGAGCAGCC TGTCGCTCAC TTCGCTACTT CTACCAAGCT CTCTAGTGCC CCTGTCTGTG 
AAAAAGTGCA ACAGCACGGG CAGCTTAGAC CAGGGGACTC TCACAGCCCA AGAAAAAGTG 
TTCACGAAAG AGCCACAGGG TAATATCACC AGCCCCTGGA AAGAGAGGAA GGAGAGGCAG 
TCGGAGGCAG CACAAGGAAC AGATGGGGAA AGTACAAGCC AAATAACAAT TGCGGGCTCT 
AGGAAAAATA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009297749.1
CDS	17..6331
Translation	MVILLNCVTLGMYQPCENIDCTSERCQVLQAFDAFIYIFFALEMVVKMVALGIFGRRCYLGDTWNRLDFFIVMAGMVEYSLDLQNINFSAIRTVRVLRPLKAINRVPSMRILVNLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWAGLLRNRCYPEENFTLTSGLTLPTPYYQPEEDDERPFICSLAQDNGIMSCSDVPARREGGRECCLDKEDALHRQALGLSAEPLVNGSASAMGLCVNWNQYYTRCHTGHTNPHKGAINFDNIGYAWIVIFQVITLEGWVEIMYYVMDAHSFYNFIYFIFLIIIGSFFMINLCLVVIATQFSETKQREHQLMQEQRARYLSSSTLASLAEPGDCYEELFQLVCHILRKARRRSAALYYMLRGKAPPPGGGRGRGKGGAGSIGGGANVNGGEKHHCHSAQTSHCIHETKLDHSSENAVSLSISPNPEECPQCAAALSLKEGEESTGHSANGEEEEGALEETDRDENRLDKKRSSDSDDTLKKKRTCLGKCKDVWDEIRVKLWGIVESKYFNRGIMIAILINTISMGIEHHNQPDELTNVLEICNIVFTSMFTLEMILKLTAFGFFEYLRNPYNIFDGIIVIISVCEIIGQSDGGLSVLRTFRLLRVIKLVRFMPALRRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHIFGCKFSLKTEAGDTVPDRKNFDSLLWAIVTVFQILTQEDWNMVLYNGMASTSPLAALYFVALMTFGNYVLFNLLVAILVEGFQAEGDANRSYSDDDRSSCNLEETEKKDSLQLSDPKISTLTPNGHLDLAPAPNARGFYPSERLSFALGSRKSSVSSLGRASLEQTTLCPGRASLYHNWGRPARPGIWSRRSSWNSLGRSSRCLGMGGGGSLRVRSPHCHPAEQESLLSPPPPPLHPPLLPRHFPPRRERRALSLELPELLQVPGPPLPPLHPRQRKKSFSGGLGSVGEHQDCNGKTPSVQPQIINEVYPQVNTRKDREDLEDELDYSLCFRIQKMMEVYRPDWCETREDWSVFLFSPQNKFRLLCQSIIAHKLFDYVVLAFIFSNCITVALERPKILQGSLERLFLTVSNYIFTAIFVGEMTLKVVSMGLYIGEQAYLRSSWNILDGFLVFVSLIDIVVSMAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLITSLKPIGNIVLICCAFFIIFGILGVQLFKGKFYYCLGLDVKNITNKSDCLLANYKWVHHKYNFDNLGQALMSLFVLASKDGWVNIMYHGLDAVAVDQQPITNNNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEVEEAKRREEKRQRRMEKKRRKAQKLPYYASYSHVRLMIHTLCTSHYLDIFITFIICVNVVTMSLEHYSQPHSLEIALKYCNYFFTSTFVLEAVLKLIAFGFRRFFKDRWNQLDLAIVLLSVMGITLEEIEISAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGELVCNEDYPCEGMSRHATFENFGMAFLTLFQVSTGDNWNGIMKDTLRECPPGEYTCNPSLQFISPLYFVSFVLTAQFVLINVVVAVLMKHLDDSNKEAQEEAEMDAEIEMELAQGTLCCIGGGVAVADRTGMGHQGAASCSVVPPHSPITHPVAHEPNSIRRLYSPAQENLWLDSVSLLIKDSFEGEMLMIDNLSGSVFHHYSSPPVCKDCRTHPQEQIHMAELEQASLRSEQLSDKSSSPALPDDLSLDEQSVYQMAAKEGKERVCDECHHSEEPGGRGNSRLRRSSRVHSSGTEDGGTCQSPHHNTPARHSIGGVPCSSSSQEHPGVSGGSSRSNTPVCLPAEFFHPAAAALPAPPRGRTGHKPRGLRLTSPASWASLRSPGANTRLLTTQYPSHSDSSLATGSSEGSLPTTMEEGLSFIVSTPQPLLDTLLDERTSLSTETLRPSPPATHILQTTRGHQRSRSSCETDINTGSIRQGSEADVGLVWCSSEQTCDSQQLSETQSSLSLTSLLLPSSLVPLSVKKCNSTGSLDQGTLTAQEKVFTKEPQGNITSPWKERKERQSEAAQGTDGESTSQITIAGSRKNR

Target ORF information:

RefSeq Version	XM_009299474.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, T type, alpha 1I subunit (cacna1i), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299474.2

ORF Insert Sequence:

ATGGTGATCC TGCTGAACTG CGTCACTCTG GGGATGTACC AGCCTTGCGA GAACATCGAC 
TGCACTTCAG AGCGCTGTCA GGTCCTACAG GCTTTTGATG CATTTATCTA CATTTTCTTT 
GCACTTGAGA TGGTGGTGAA GATGGTGGCT CTGGGGATCT TTGGCCGTCG CTGCTATCTT 
GGCGATACTT GGAACAGGCT GGACTTTTTC ATCGTGATGG CAGGGATGGT GGAATACTCC 
CTTGATCTCC AGAACATCAA CTTCTCCGCC ATCAGAACAG TGCGTGTGCT AAGACCTCTC 
AAAGCCATCA ACAGAGTACC CAGTATGCGC ATCCTGGTGA ACCTGCTGCT GGACACTCTG 
CCCATGCTGG GAAATGTGCT CCTGCTTTGT TTCTTTGTGT TCTTCATTTT TGGCATCATT 
GGTGTACAAC TGTGGGCTGG ACTCCTAAGA AATCGGTGTT ACCCAGAGGA GAACTTCACC 
CTCACATCTG GCCTCACCCT TCCCACACCA TATTATCAGC CTGAGGAGGA TGACGAGCGG 
CCCTTCATCT GCTCGTTGGC TCAAGATAAC GGCATCATGT CCTGCAGCGA TGTGCCTGCT 
CGGAGAGAGG GCGGACGGGA GTGCTGTCTG GATAAAGAGG ACGCTCTTCA CCGGCAGGCT 
CTGGGTCTGA GCGCCGAGCC GCTGGTCAAC GGCAGCGCAT CTGCCATGGG CCTCTGTGTG 
AACTGGAATC AGTACTACAC CCGCTGCCAC ACTGGACACA CCAATCCTCA TAAAGGAGCA 
ATTAATTTCG ACAACATAGG ATACGCATGG ATCGTCATAT TTCAGGTGAT CACGTTAGAG 
GGCTGGGTGG AAATCATGTA CTACGTCATG GACGCGCATT CCTTCTACAA CTTCATCTAT 
TTTATATTCC TCATCATCAT TGGATCTTTC TTCATGATTA ATCTGTGCCT GGTGGTCATT 
GCCACTCAGT TTTCGGAGAC CAAACAGCGA GAGCACCAGC TGATGCAAGA GCAACGTGCT 
CGCTACCTGT CCTCCAGCAC GCTGGCTTCA CTCGCTGAGC CCGGAGACTG TTACGAGGAG 
CTCTTCCAAC TGGTCTGCCA TATCCTACGC AAAGCCCGCC GCCGCTCTGC TGCCCTTTAT 
TACATGCTCC GTGGCAAAGC CCCTCCCCCT GGAGGTGGGA GGGGGCGTGG CAAAGGAGGA 
GCAGGATCGA TAGGAGGAGG AGCCAATGTT AATGGAGGAG AGAAACATCA CTGCCATTCA 
GCTCAGACAT CTCACTGTAT TCACGAAACC AAATTGGACC ACAGCTCAGA GAATGCAGTC 
TCCCTCTCCA TCAGTCCAAA CCCAGAGGAG TGTCCACAGT GTGCTGCAGC CCTGTCTCTC 
AAAGAGGGGG AGGAGTCAAC AGGGCATTCA GCCAATGGGG AGGAAGAGGA GGGTGCGCTG 
GAGGAAACTG ACAGGGACGA GAACCGCTTA GACAAAAAGA GGAGCAGCGA CTCTGATGAC 
ACGCTAAAGA AGAAACGCAC CTGTTTGGGG AAATGTAAAG ACGTCTGGGA CGAAATAAGA 
GTGAAACTTT GGGGCATCGT TGAAAGCAAG TACTTCAACA GAGGGATTAT GATTGCCATC 
CTCATCAATA CAATCAGCAT GGGCATCGAG CACCATAACC AGCCTGATGA ACTGACTAAT 
GTCCTGGAGA TCTGTAACAT AGTGTTCACC AGTATGTTCA CTCTGGAGAT GATCCTCAAG 
CTCACTGCCT TTGGCTTCTT TGAATATCTG AGAAACCCTT ACAACATCTT TGACGGCATC 
ATTGTGATCA TCAGTGTATG TGAGATCATT GGTCAGTCTG ACGGAGGTCT GTCAGTCCTG 
AGGACGTTCA GATTGCTGAG GGTGATAAAG CTGGTGCGGT TCATGCCAGC TCTGCGCAGG 
CAGCTGGTGG TCCTGATGAA GACCATGGAC AATGTGGCCA CGTTCTGCAT GCTGCTAATG 
CTCTTCATCT TTATATTCAG TATTCTGGGA ATGCACATAT TTGGCTGCAA GTTCAGCCTG 
AAGACAGAGG CTGGGGACAC CGTACCTGAC AGGAAGAACT TTGACTCTCT GCTTTGGGCC 
ATTGTTACAG TGTTTCAGAT CTTAACTCAA GAGGACTGGA ACATGGTTTT GTATAACGGC 
ATGGCCTCCA CCTCGCCTCT GGCAGCCCTG TATTTCGTCG CGCTCATGAC TTTTGGAAAC 
TACGTTCTAT TCAATCTGCT TGTGGCCATC TTGGTTGAAG GATTTCAGGC TGAGGGCGAT 
GCTAACCGTT CCTACTCGGA TGATGACAGA TCATCTTGTA ATTTAGAAGA AACCGAGAAA 
AAGGACTCTC TCCAGCTGTC AGATCCCAAG ATTTCCACGT TGACCCCTAA CGGGCATCTG 
GATTTAGCAC CTGCACCCAA TGCTAGGGGC TTTTACCCAT CTGAAAGGCT GAGCTTTGCC 
CTGGGTTCGC GCAAGAGCAG CGTTAGCTCT CTGGGCAGGG CTAGCCTGGA GCAAACCACG 
CTGTGTCCAG GACGAGCATC TCTTTACCAC AATTGGGGAC GCCCCGCACG GCCAGGAATA 
TGGAGCCGCA GGTCGAGTTG GAACAGCTTG GGACGGTCCT CCAGATGTCT GGGGATGGGG 
GGTGGAGGCA GTTTACGAGT CCGTAGCCCT CACTGTCATC CCGCCGAGCA GGAATCCCTG 
CTGTCTCCTC CACCTCCGCC TCTCCATCCG CCTCTGCTCC CCAGACACTT TCCCCCACGC 
AGGGAACGCA GGGCTCTGTC TCTGGAGCTC CCAGAGCTTC TACAGGTGCC AGGACCACCC 
CTGCCTCCTC TGCACCCCCG CCAGCGCAAG AAGAGCTTCT CTGGGGGTCT GGGAAGCGTT 
GGAGAGCATC AGGACTGTAA TGGGAAAACG CCATCAGTTC AACCACAAAT CATTAATGAA 
GTCTACCCTC AGGTCAACAC ACGCAAGGAC AGGGAGGATC TAGAGGATGA GCTAGACTAT 
AGTCTGTGTT TCCGGATCCA GAAGATGATG GAGGTGTACA GACCGGACTG GTGTGAGACC 
AGGGAAGACT GGTCTGTCTT CCTCTTCTCT CCTCAAAACA AGTTCAGGTT GTTGTGTCAG 
TCCATCATAG CCCACAAGCT CTTTGACTAT GTGGTGCTGG CCTTCATCTT CTCAAACTGC 
ATCACTGTGG CTCTGGAGAG ACCCAAGATC CTTCAGGGTA GCCTGGAGAG GCTTTTCCTT 
ACCGTGTCCA ATTACATTTT CACAGCCATA TTTGTGGGGG AGATGACGCT CAAGGTGGTG 
TCTATGGGTT TGTATATTGG AGAGCAGGCA TACTTGCGCA GCAGCTGGAA TATTTTGGAT 
GGTTTCCTGG TGTTTGTGTC TTTGATTGAT ATTGTGGTGT CGATGGCAGG CGGGGCTAAG 
ATCCTGGGGG TGCTCAGGGT CCTCAGGCTT CTTCGCACAC TGCGCCCTCT CAGGGTTATC 
AGTCGAGCTC CTGGTCTCAA ACTAGTTGTG GAGACTTTGA TCACCTCTCT TAAGCCTATT 
GGAAACATAG TTCTCATCTG CTGTGCCTTT TTCATTATTT TTGGGATCCT GGGAGTTCAG 
CTTTTCAAAG GCAAGTTCTA CTACTGCTTG GGCCTGGATG TCAAAAACAT CACCAACAAG 
TCTGACTGCC TCTTGGCCAA TTATAAGTGG GTCCATCACA AGTACAACTT TGACAACTTG 
GGGCAGGCTC TGATGTCCTT GTTTGTACTG GCTTCGAAGG ACGGCTGGGT CAACATCATG 
TATCATGGTT TGGATGCTGT GGCAGTGGAC CAACAGCCCA TAACCAACAA CAACCCCTGG 
ATGCTGCTGT ACTTCATCTC ATTCCTCTTG ATCGTCAGTT TCTTCGTGCT GAATATGTTT 
GTTGGAGTGG TGGTGGAGAA CTTCCATAAA TGCCGACAGC ATCAGGAGGT GGAGGAGGCC 
AAGAGGAGGG AGGAGAAGAG ACAGAGACGC ATGGAGAAAA AGAGACGGAA GGCCCAGAAG 
TTGCCCTACT ATGCCAGTTA TAGTCATGTA CGGCTGATGA TCCACACCCT GTGCACCAGT 
CACTACCTGG ATATCTTCAT CACCTTCATC ATCTGTGTGA ATGTAGTCAC CATGTCACTG 
GAGCACTACA GCCAGCCTCA TTCTCTGGAG ATCGCACTGA AGTACTGTAA TTATTTCTTC 
ACCAGCACCT TTGTGCTGGA GGCTGTCCTG AAGCTCATCG CATTTGGCTT CCGACGTTTC 
TTCAAAGACA GGTGGAACCA GCTGGATTTG GCAATTGTAC TGCTGTCTGT CATGGGCATC 
ACGCTGGAGG AGATTGAAAT CAGTGCAGCT CTGCCCATCA ACCCCACCAT AATACGTATC 
ATGAGGGTCC TACGCATCGC ACGTGTGTTA AAGCTGTTAA AGATGGCCAC AGGAATGAGA 
GCGCTGCTGG ATACAGTAGT TCAAGCCCTG CCACAGGTGG GGAACCTTGG CCTCCTCTTC 
ATGCTGCTGT TCTTCATCTA TGCTGCACTG GGGGTGGAAT TGTTCGGAGA GCTGGTGTGC 
AATGAAGATT ACCCATGTGA AGGCATGAGT CGTCATGCCA CCTTTGAGAA CTTCGGCATG 
GCGTTCCTCA CCCTTTTCCA GGTCTCCACT GGCGATAACT GGAATGGCAT AATGAAGGAC 
ACTCTCCGTG AATGTCCGCC AGGCGAATAC ACCTGTAATC CCAGCCTACA ATTCATCTCC 
CCGCTGTACT TTGTGAGCTT TGTGCTCACG GCCCAGTTTG TCCTCATCAA TGTGGTGGTG 
GCGGTGCTGA TGAAACACCT GGACGACTCA AACAAGGAGG CCCAGGAGGA GGCAGAGATG 
GATGCCGAGA TTGAAATGGA GCTGGCCCAG GGAACGCTCT GCTGCATCGG CGGAGGGGTA 
GCCGTAGCCG ACAGGACCGG CATGGGGCAT CAGGGGGCTG CATCCTGCTC CGTTGTTCCC 
CCTCACTCTC CAATCACACA CCCAGTGGCC CACGAACCCA ACAGCATCAG GAGACTCTAC 
TCACCGGCGC AGGAGAACTT GTGGCTGGAC AGTGTATCTC TGCTCATCAA AGATTCATTT 
GAAGGAGAGA TGCTGATGAT TGACAACCTC TCTGGCTCCG TCTTCCATCA TTACTCCTCT 
CCTCCTGTCT GCAAGGACTG CAGGACTCAT CCGCAAGAGC AGATCCACAT GGCTGAACTG 
GAGCAGGCGT CTCTGAGGTC GGAGCAGCTC TCAGATAAGT CATCCTCGCC AGCGCTGCCC 
GATGACCTCA GCCTGGACGA GCAGAGCGTC TACCAGATGG CTGCCAAAGA GGGCAAGGAG 
AGAGTCTGTG ATGAGTGCCA TCATTCAGAA GAGCCAGGAG GGAGAGGAAA CAGCAGGTTA 
CGACGATCCT CTCGCGTCCA CAGCTCTGGG ACAGAAGATG GAGGAACTTG TCAAAGTCCA 
CATCACAACA CACCTGCTCG ACACAGCATA GGTGGCGTTC CCTGTAGCAG CAGTAGTCAA 
GAGCACCCAG GGGTTTCTGG AGGTTCGAGC CGCTCTAACA CCCCCGTTTG TCTCCCTGCT 
GAATTCTTCC ACCCAGCTGC TGCGGCTCTG CCTGCACCTC CGAGAGGCCG AACAGGACAC 
AAACCAAGAG GCCTTCGGCT AACTTCACCA GCCTCTTGGG CTTCTCTGCG CTCTCCTGGA 
GCAAACACCA GGCTGCTCAC CACACAGTAC CCATCTCACA GTGACTCCTC TCTGGCTACG 
GGCAGCTCAG AGGGCAGCCT ACCGACCACC ATGGAAGAAG GGCTCAGTTT CATCGTATCA 
ACGCCCCAGC CCCTTCTGGA CACACTCCTC GACGAGCGTA CTAGCCTCTC CACAGAGACC 
CTGAGACCCA GTCCTCCAGC CACACACATC CTCCAAACCA CTAGGGGGCA CCAGCGCAGC 
AGGAGCAGCT GTGAAACGGA CATCAATACG GGCAGCATTC GACAAGGTTC CGAGGCGGAT 
GTAGGATTGG TATGGTGCAG CTCCGAGCAG ACATGCGACA GCCAGCAGCT CTCAGAGACG 
CAGAGCAGCC TGTCGCTCAC TTCGCTACTT CTACCAAGCT CTCTAGTGCC CCTGTCTGTG 
AAAAAGTGCA ACAGCACGGG CAGCTTAGAC CAGGGGACTC TCACAGCCCA AGAAAAAGTG 
TTCACGAAAG AGCCACAGGG TAATATCACC AGCCCCTGGA AAGAGAGGAA GGAGAGGCAG 
TCGGAGGCAG CACAAGGAAC AGATGGGGAA AGTACAAGCC AAATAACAAT TGCGGGCTCT 
AGGAAAAATA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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cacna1i cDNA ORF clone, Danio rerio(zebrafish)

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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