prtg cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	prtg
Entrez Gene ID	100496178
Full Name	protogenin
Synonyms	igdcc5
General protein information	Preferred Names protogenin Names protogenin
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004912700.4	XP_004912757.1	protogenin isoform X1
XM_002940254.4	XP_002940300.2	protogenin isoform X2
XM_004912700.3	XP_004912757.1	protogenin isoform X1
XM_002940254.5	XP_002940300.2	protogenin isoform X2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PRTG	XP_544694.3
Bos taurus (cattle)	PRTG	XP_005211829.1
Mus musculus (house mouse)	Prtg	NP_780694.3
Xenopus tropicalis (tropical clawed frog)	prtg	XP_004912757.1
Homo sapiens (human)	PRTG	NP_776175.2
Gallus gallus (chicken)	PRTG	NP_001025719.1
Pan troglodytes (chimpanzee)	PRTG	XP_510430.2
	PRTG	XP_001090352.1
Rattus norvegicus (Norway rat)	Prtg	NP_001032740.1
Danio rerio (zebrafish)	prtga	NP_001038495.1

Gene Ontology

The following prtg gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the prtg cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa07372	XM_004912700.4 Latest version!	Xenopus tropicalis protogenin (prtg), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa07373	XM_002940254.5 Latest version!	Xenopus tropicalis protogenin (prtg), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OXa07372	XM_004912700.3	Xenopus tropicalis protogenin (prtg), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa07373	XM_002940254.4	Xenopus tropicalis protogenin (prtg), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

prtg ( XM_004912700.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912757.1 Xenopus tropicalis protogenin (prtg), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa07372
Clone ID Related Accession (Same CDS sequence)	XM_004912700.4 , XM_004912700.3
Accession Version	XM_004912700.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protogenin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004912700.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTT GCAGAAGGCG ACTTCCCCAC GGGGTACTCT ATTGGGTAAC CCTGTTCAGC 
CTCCCAGGGA TTTTGTGTTT TAGTGATCTG ATGTTTGTTA AAGAACCTCA AGATGTAATC 
ATTTTACGGA GAGATTCAGT GGTTTTAGAC TGCCTGGCTA ATGGAGAAGC TCCAATAACA 
GTCACGTGGA TGAAGAACGG AGTAAAGATT TCTGAGAATG AACGGATATC CACATTACAC 
AATGGATCCT TGTACATCCG TGAGGCAGAG GGCAGGAAAG GAGATCAATC TGATGAAGGA 
TTTTATCAGT GTTTGGCTTT GAACAAATTT GGTGCCATTC TAAGTCAAAG AGCTCATCTT 
TCATTTGCAA GCATGTCAGG ATTTGAAGAA CAACCTGTGT CTGTTCAGGT CCAACATGGA 
AGTGTGGCTC GATTCTCCTG TAAGATAACA GCTAATCCTC CAGCAGTCAT TACCTGGGAA 
GTAAATCGGA CTGCTCTGCC CCTTGCTATG GACAGGATAA CTGTGCTATC AAAAGGAGTT 
CTCCAGATCT ATGGAGTCAC GAAGAGGGAT GCTGGGAATT ACAGATGTGT AGCCACCAGT 
GTTTCAAACA GGCGGAAGAG CACAGAAGCT GTGCTAACTG TTGTGCCAGG AGAGAGACTG 
AATCCAAGGC ACCATCTTTC CCAAACACAA CTCACTCTGA GAGCTAAGGA AAAGAAATAT 
TTTCAGAAAC CAGTCATCAT TGCTCCTCCA CAGAATATAA CTACCTCTAT GCACCAGTCG 
GTCACTCTGG AATGTGTGGC TACAGGGAAC CCAAAGCCTA TTGTATCATG GAGCAGGAGG 
GACCATAAAC CAATTGATGT GTTTAACACA CGAGTAGTTG GAAATGGAAA CCTCATAATA 
TCAGATGTTA AGCCGCACCA CTCTGGGGTC TACATTTGTC GTGCCACCAC TCCCGGTACA 
CGCAACTTTA CTGTTGCAAT GACAACTGTC ACAGTTCTAG CACCTCCATC CTTTACTGAA 
TGGCCAGCCA GTTTGACAAT GCCACGTGCT AGTACTGCCA GATTTGTATG TCAAGCCGAA 
GGGAATCCAG TTCCAAAAAT TTCATGGCTA AAGAATGGCC AAAAAATTAA TTCCAATGGC 
AGAATTAAAA TGTACAACAG TAAACTGGTA ATTAGCCATA TAATGCCTGA AGATGATGCA 
GTGTATCAGT GCATGGCAGA GAATAGTCAA GGATCTGTTC TTTCTCGCGC ACGGTTGATA 
GTCGTTATGT ATGAGCACAG ACCCAGTGCT CCACGTCAGC TTCGGGCTGA GACAATGTCC 
AGTTCAGCCA TCATTCTCTC TTGGGAACGG CCTCTGTATA ACTCAGAAAA AATCATTGCT 
TATTCTGTGC ATTACATGAA AGCAGAAGGT TTAAATAATG AAGAATATCA GGTGGTAATT 
GGAAATGACA CAACTCATTA CATCATTGAT GACTTGGATC CTGCCAGCAA TTTTACCTTT 
TACGTTGTAG CATATATGCC AATGGGGGCA AGCCAAATGT CTGATCATGT GACACAGAGC 
ACTCTTGAAG ATGTTCCTTT AAGAGCTCCA GAAATCAGTT TAACAAGCCG CAGTCCAACA 
GATGTCCTTA TTTCTTGGCT TCCTATTTCG GCCAAATTCC GTCGGGGAAA GATTATCCAT 
TACAGACTGT CTTATCGAAT GAGCACTGAA AACACCACCC ATAATGTAGA GCTCCCGGGA 
AGCACACATG AGTATTTCTT GGAAGGCTTG AATTCTGATA CCCTGTATCT AGTCCGTATA 
GCTGCAGCAA CTAAAGTAGG ATGGGGGGAA TCTTCTGCAT GGACATCTCA CAGGACACCT 
AAGTCTACAA GTATAAGAGC ACCGAGGTCT CCGGAGCTCC GTTTGGACCC CTTGAATTGC 
ACAACCATAA CAGTCAGATG GCAGCAAGAC CCTGACGATA CTGCTACCAT ACAGGGCTAC 
AAACTGTATT ATAAAGAAGA AGGACAGCAA GAAAGTGGGC CAGTACTTCT GGATGTGAAA 
GATTTTGATT ATAACATAGG AGGGTTAGAT CCAAGAAAAA AATATCATGT GAGACTACTG 
GCCTACAACA ACATGGATGA AGGCTACCAA GCAGACCAAA CAGTCAGCAC ACCAGTCTGT 
GTGTCTGCTC CTGATCACGT AAATCCTCCA CCGCCACCAC CTCATCATCT CTATGCTAAA 
GCAAACAGCT CTTCTTCCAT TTACCTGCAC TGGGGTCGAC CAGCCTTCTC CTCTGCCAAG 
GCCATTAACT ACACTGTCCG CTGCAATCCT GTTGGCCTGC AGAACGCTTC CTTAGTGCTT 
TACCTACAGA CGACAGAATC TCACATTTTA CTTCAGGATC TTGAAGCTAA CAAAAAATAT 
GAATTTGCTG TGAGGCTTCA TGTGGACCTT TTGTCAAGCC CCTGGAGCCC TGTGGTTTAC 
CAGTCCACCC TCCCTGATGC TCCACCAAGG CCACCAGTGG GAGTAAAGGT GACCTTAATA 
GAAGAAAACA CTGCTTTGGT CTCCTGGAAG CCCCCTGAAG AATTGGATTC AGCTGTGACC 
AGTTATACCA TCCTGTACAC GTCTAGAAAG TCCTGGATTG CTGGCGAGTG GCAAATTATG 
CATAGAGAAG GAACAATGAC CATGGCATTG TTAGAGAATC TGCTCCCTGG AAACATTTAC 
CTTGTCAAGA TTTCAGCTGC TAATGAAGCT GGAGAGGGAC CCTTCTCTAA TGTTGTTGAG 
TTGGTAGTGG AGTCAAAGGA GAATCTGCGA GTAGTCACAA GGTCAAAGCA GTTAGATTCT 
GGACATCGTG AAGATTATTC CAGTTTTTAC CACTTGGATC AGAAATCTAT GACTGGCATT 
GTTGTCGGAG TGTGTATTGC ATTAACTTGT ATCCTGATTT GTGTCCTCAT CTTGATTTAC 
CGAAGCAAAG CTAGGAAAGC ATCAATTGCT AAGACTGCTC AGCAAGAACC CCACCTTCTA 
CCTCATGCAA CTGCATCAGC TCCTACTGAT AGGCAGGAGA GAAAAGCAAC AGATCAAGTG 
GAGGGAAGTA CCGGCCCCTT GATCCCTTCT GTGGAATCTA ACCACTTTAT AGATGCAAAG 
GGTGGCTCAA ATCTTGTCAT AAATGGCTAC GGGCCAGTTA AAAATAACAC CAAGAAATCA 
TGGCCATTTT TCAAACACAA TAAGAATGGC TCTGTTGAAC AGCCCAACAA AAGAATTGTC 
AAAGCAATGT GCTTATATCA GCCTGGCAAT AGAGTGTTGA TAGGAGAAGA TTTATCCCCT 
GCATCTCCAG GAAATCCTGG AACATATAAA ATGCGGAACA GCATTGCTGA TACAGAGCAT 
TCTGGAAACA GTGATGAAAG TCATGAAACC AGTGATTCTG GACGGTTTTC TCATGAATCC 
ACTGATGAAA TTCACCTGTA CTCAGTTGTA TGTCCCACCA CTCCTACATC AGATTCTTTC 
ACCAGCAATG ACTGTAGACA GAACTCTGCT TTATATGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912757.1
CDS	449..3970
Translation	MAACRRRLPHGVLYWVTLFSLPGILCFSDLMFVKEPQDVIILRRDSVVLDCLANGEAPITVTWMKNGVKISENERISTLHNGSLYIREAEGRKGDQSDEGFYQCLALNKFGAILSQRAHLSFASMSGFEEQPVSVQVQHGSVARFSCKITANPPAVITWEVNRTALPLAMDRITVLSKGVLQIYGVTKRDAGNYRCVATSVSNRRKSTEAVLTVVPGERLNPRHHLSQTQLTLRAKEKKYFQKPVIIAPPQNITTSMHQSVTLECVATGNPKPIVSWSRRDHKPIDVFNTRVVGNGNLIISDVKPHHSGVYICRATTPGTRNFTVAMTTVTVLAPPSFTEWPASLTMPRASTARFVCQAEGNPVPKISWLKNGQKINSNGRIKMYNSKLVISHIMPEDDAVYQCMAENSQGSVLSRARLIVVMYEHRPSAPRQLRAETMSSSAIILSWERPLYNSEKIIAYSVHYMKAEGLNNEEYQVVIGNDTTHYIIDDLDPASNFTFYVVAYMPMGASQMSDHVTQSTLEDVPLRAPEISLTSRSPTDVLISWLPISAKFRRGKIIHYRLSYRMSTENTTHNVELPGSTHEYFLEGLNSDTLYLVRIAAATKVGWGESSAWTSHRTPKSTSIRAPRSPELRLDPLNCTTITVRWQQDPDDTATIQGYKLYYKEEGQQESGPVLLDVKDFDYNIGGLDPRKKYHVRLLAYNNMDEGYQADQTVSTPVCVSAPDHVNPPPPPPHHLYAKANSSSSIYLHWGRPAFSSAKAINYTVRCNPVGLQNASLVLYLQTTESHILLQDLEANKKYEFAVRLHVDLLSSPWSPVVYQSTLPDAPPRPPVGVKVTLIEENTALVSWKPPEELDSAVTSYTILYTSRKSWIAGEWQIMHREGTMTMALLENLLPGNIYLVKISAANEAGEGPFSNVVELVVESKENLRVVTRSKQLDSGHREDYSSFYHLDQKSMTGIVVGVCIALTCILICVLILIYRSKARKASIAKTAQQEPHLLPHATASAPTDRQERKATDQVEGSTGPLIPSVESNHFIDAKGGSNLVINGYGPVKNNTKKSWPFFKHNKNGSVEQPNKRIVKAMCLYQPGNRVLIGEDLSPASPGNPGTYKMRNSIADTEHSGNSDESHETSDSGRFSHESTDEIHLYSVVCPTTPTSDSFTSNDCRQNSALYE

Target ORF information:

RefSeq Version	XM_004912700.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis protogenin (prtg), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912700.4

ORF Insert Sequence:

ATGGCGGCTT GCAGAAGGCG ACTTCCCCAC GGGGTACTCT ATTGGGTAAC CCTGTTCAGC 
CTCCCAGGGA TTTTGTGTTT TAGTGATCTG ATGTTTGTTA AAGAACCTCA AGATGTAATC 
ATTTTACGGA GAGATTCAGT GGTTTTAGAC TGCCTGGCTA ATGGAGAAGC TCCAATAACA 
GTCACGTGGA TGAAGAACGG AGTAAAGATT TCTGAGAATG AACGGATATC CACATTACAC 
AATGGATCCT TGTACATCCG TGAGGCAGAG GGCAGGAAAG GAGATCAATC TGATGAAGGA 
TTTTATCAGT GTTTGGCTTT GAACAAATTT GGTGCCATTC TAAGTCAAAG AGCTCATCTT 
TCATTTGCAA GCATGTCAGG ATTTGAAGAA CAACCTGTGT CTGTTCAGGT CCAACATGGA 
AGTGTGGCTC GATTCTCCTG TAAGATAACA GCTAATCCTC CAGCAGTCAT TACCTGGGAA 
GTAAATCGGA CTGCTCTGCC CCTTGCTATG GACAGGATAA CTGTGCTATC AAAAGGAGTT 
CTCCAGATCT ATGGAGTCAC GAAGAGGGAT GCTGGGAATT ACAGATGTGT AGCCACCAGT 
GTTTCAAACA GGCGGAAGAG CACAGAAGCT GTGCTAACTG TTGTGCCAGG AGAGAGACTG 
AATCCAAGGC ACCATCTTTC CCAAACACAA CTCACTCTGA GAGCTAAGGA AAAGAAATAT 
TTTCAGAAAC CAGTCATCAT TGCTCCTCCA CAGAATATAA CTACCTCTAT GCACCAGTCG 
GTCACTCTGG AATGTGTGGC TACAGGGAAC CCAAAGCCTA TTGTATCATG GAGCAGGAGG 
GACCATAAAC CAATTGATGT GTTTAACACA CGAGTAGTTG GAAATGGAAA CCTCATAATA 
TCAGATGTTA AGCCGCACCA CTCTGGGGTC TACATTTGTC GTGCCACCAC TCCCGGTACA 
CGCAACTTTA CTGTTGCAAT GACAACTGTC ACAGTTCTAG CACCTCCATC CTTTACTGAA 
TGGCCAGCCA GTTTGACAAT GCCACGTGCT AGTACTGCCA GATTTGTATG TCAAGCCGAA 
GGGAATCCAG TTCCAAAAAT TTCATGGCTA AAGAATGGCC AAAAAATTAA TTCCAATGGC 
AGAATTAAAA TGTACAACAG TAAACTGGTA ATTAGCCATA TAATGCCTGA AGATGATGCA 
GTGTATCAGT GCATGGCAGA GAATAGTCAA GGATCTGTTC TTTCTCGCGC ACGGTTGATA 
GTCGTTATGT ATGAGCACAG ACCCAGTGCT CCACGTCAGC TTCGGGCTGA GACAATGTCC 
AGTTCAGCCA TCATTCTCTC TTGGGAACGG CCTCTGTATA ACTCAGAAAA AATCATTGCT 
TATTCTGTGC ATTACATGAA AGCAGAAGGT TTAAATAATG AAGAATATCA GGTGGTAATT 
GGAAATGACA CAACTCATTA CATCATTGAT GACTTGGATC CTGCCAGCAA TTTTACCTTT 
TACGTTGTAG CATATATGCC AATGGGGGCA AGCCAAATGT CTGATCATGT GACACAGAGC 
ACTCTTGAAG ATGTTCCTTT AAGAGCTCCA GAAATCAGTT TAACAAGCCG CAGTCCAACA 
GATGTCCTTA TTTCTTGGCT TCCTATTTCG GCCAAATTCC GTCGGGGAAA GATTATCCAT 
TACAGACTGT CTTATCGAAT GAGCACTGAA AACACCACCC ATAATGTAGA GCTCCCGGGA 
AGCACACATG AGTATTTCTT GGAAGGCTTG AATTCTGATA CCCTGTATCT AGTCCGTATA 
GCTGCAGCAA CTAAAGTAGG ATGGGGGGAA TCTTCTGCAT GGACATCTCA CAGGACACCT 
AAGTCTACAA GTATAAGAGC ACCGAGGTCT CCGGAGCTCC GTTTGGACCC CTTGAATTGC 
ACAACCATAA CAGTCAGATG GCAGCAAGAC CCTGACGATA CTGCTACCAT ACAGGGCTAC 
AAACTGTATT ATAAAGAAGA AGGACAGCAA GAAAGTGGGC CAGTACTTCT GGATGTGAAA 
GATTTTGATT ATAACATAGG AGGGTTAGAT CCAAGAAAAA AATATCATGT GAGACTACTG 
GCCTACAACA ACATGGATGA AGGCTACCAA GCAGACCAAA CAGTCAGCAC ACCAGTCTGT 
GTGTCTGCTC CTGATCACGT AAATCCTCCA CCGCCACCAC CTCATCATCT CTATGCTAAA 
GCAAACAGCT CTTCTTCCAT TTACCTGCAC TGGGGTCGAC CAGCCTTCTC CTCTGCCAAG 
GCCATTAACT ACACTGTCCG CTGCAATCCT GTTGGCCTGC AGAACGCTTC CTTAGTGCTT 
TACCTACAGA CGACAGAATC TCACATTTTA CTTCAGGATC TTGAAGCTAA CAAAAAATAT 
GAATTTGCTG TGAGGCTTCA TGTGGACCTT TTGTCAAGCC CCTGGAGCCC TGTGGTTTAC 
CAGTCCACCC TCCCTGATGC TCCACCAAGG CCACCAGTGG GAGTAAAGGT GACCTTAATA 
GAAGAAAACA CTGCTTTGGT CTCCTGGAAG CCCCCTGAAG AATTGGATTC AGCTGTGACC 
AGTTATACCA TCCTGTACAC GTCTAGAAAG TCCTGGATTG CTGGCGAGTG GCAAATTATG 
CATAGAGAAG GAACAATGAC CATGGCATTG TTAGAGAATC TGCTCCCTGG AAACATTTAC 
CTTGTCAAGA TTTCAGCTGC TAATGAAGCT GGAGAGGGAC CCTTCTCTAA TGTTGTTGAG 
TTGGTAGTGG AGTCAAAGGA GAATCTGCGA GTAGTCACAA GGTCAAAGCA GTTAGATTCT 
GGACATCGTG AAGATTATTC CAGTTTTTAC CACTTGGATC AGAAATCTAT GACTGGCATT 
GTTGTCGGAG TGTGTATTGC ATTAACTTGT ATCCTGATTT GTGTCCTCAT CTTGATTTAC 
CGAAGCAAAG CTAGGAAAGC ATCAATTGCT AAGACTGCTC AGCAAGAACC CCACCTTCTA 
CCTCATGCAA CTGCATCAGC TCCTACTGAT AGGCAGGAGA GAAAAGCAAC AGATCAAGTG 
GAGGGAAGTA CCGGCCCCTT GATCCCTTCT GTGGAATCTA ACCACTTTAT AGATGCAAAG 
GGTGGCTCAA ATCTTGTCAT AAATGGCTAC GGGCCAGTTA AAAATAACAC CAAGAAATCA 
TGGCCATTTT TCAAACACAA TAAGAATGGC TCTGTTGAAC AGCCCAACAA AAGAATTGTC 
AAAGCAATGT GCTTATATCA GCCTGGCAAT AGAGTGTTGA TAGGAGAAGA TTTATCCCCT 
GCATCTCCAG GAAATCCTGG AACATATAAA ATGCGGAACA GCATTGCTGA TACAGAGCAT 
TCTGGAAACA GTGATGAAAG TCATGAAACC AGTGATTCTG GACGGTTTTC TCATGAATCC 
ACTGATGAAA TTCACCTGTA CTCAGTTGTA TGTCCCACCA CTCCTACATC AGATTCTTTC 
ACCAGCAATG ACTGTAGACA GAACTCTGCT TTATATGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prtg ( XM_002940254.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002940300.2 Xenopus tropicalis protogenin (prtg), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa07373
Clone ID Related Accession (Same CDS sequence)	XM_002940254.4 , XM_002940254.5
Accession Version	XM_002940254.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protogenin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002940254.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTT GCAGAAGGCG ACTTCCCCAC GGGGTACTCT ATTGGGTAAC CCTGTTCAGC 
CTCCCAGGGA TTTTGTGTTT TAGTGATCTG ATGTTTGTTA AAGAACCTCA AGATGTAATC 
ATTTTACGGA GAGATTCAGT GGTTTTAGAC TGCCTGGCTA ATGGAGAAGC TCCAATAACA 
GTCACGTGGA TGAAGAACGG AGTAAAGATT TCTGAGAATG AACGGATATC CACATTACAC 
AATGGATCCT TGTACATCCG TGAGGCAGAG GGCAGGAAAG GAGATCAATC TGATGAAGGA 
TTTTATCAGT GTTTGGCTTT GAACAAATTT GGTGCCATTC TAAGTCAAAG AGCTCATCTT 
TCATTTGCAA GCATGTCAGG ATTTGAAGAA CAACCTGTGT CTGTTCAGGT CCAACATGGA 
AGTGTGGCTC GATTCTCCTG TAAGATAACA GCTAATCCTC CAGCAGTCAT TACCTGGGAA 
GTAAATCGGA CTGCTCTGCC CCTTGCTATG GACAGGATAA CTGTGCTATC AAAAGGAGTT 
CTCCAGATCT ATGGAGTCAC GAAGAGGGAT GCTGGGAATT ACAGATGTGT AGCCACCAGT 
GTTTCAAACA GGCGGAAGAG CACAGAAGCT GTGCTAACTG TTGTGCCAGC TAAGGAAAAG 
AAATATTTTC AGAAACCAGT CATCATTGCT CCTCCACAGA ATATAACTAC CTCTATGCAC 
CAGTCGGTCA CTCTGGAATG TGTGGCTACA GGGAACCCAA AGCCTATTGT ATCATGGAGC 
AGGAGGGACC ATAAACCAAT TGATGTGTTT AACACACGAG TAGTTGGAAA TGGAAACCTC 
ATAATATCAG ATGTTAAGCC GCACCACTCT GGGGTCTACA TTTGTCGTGC CACCACTCCC 
GGTACACGCA ACTTTACTGT TGCAATGACA ACTGTCACAG TTCTAGCACC TCCATCCTTT 
ACTGAATGGC CAGCCAGTTT GACAATGCCA CGTGCTAGTA CTGCCAGATT TGTATGTCAA 
GCCGAAGGGA ATCCAGTTCC AAAAATTTCA TGGCTAAAGA ATGGCCAAAA AATTAATTCC 
AATGGCAGAA TTAAAATGTA CAACAGTAAA CTGGTAATTA GCCATATAAT GCCTGAAGAT 
GATGCAGTGT ATCAGTGCAT GGCAGAGAAT AGTCAAGGAT CTGTTCTTTC TCGCGCACGG 
TTGATAGTCG TTATGTATGA GCACAGACCC AGTGCTCCAC GTCAGCTTCG GGCTGAGACA 
ATGTCCAGTT CAGCCATCAT TCTCTCTTGG GAACGGCCTC TGTATAACTC AGAAAAAATC 
ATTGCTTATT CTGTGCATTA CATGAAAGCA GAAGGTTTAA ATAATGAAGA ATATCAGGTG 
GTAATTGGAA ATGACACAAC TCATTACATC ATTGATGACT TGGATCCTGC CAGCAATTTT 
ACCTTTTACG TTGTAGCATA TATGCCAATG GGGGCAAGCC AAATGTCTGA TCATGTGACA 
CAGAGCACTC TTGAAGATGT TCCTTTAAGA GCTCCAGAAA TCAGTTTAAC AAGCCGCAGT 
CCAACAGATG TCCTTATTTC TTGGCTTCCT ATTTCGGCCA AATTCCGTCG GGGAAAGATT 
ATCCATTACA GACTGTCTTA TCGAATGAGC ACTGAAAACA CCACCCATAA TGTAGAGCTC 
CCGGGAAGCA CACATGAGTA TTTCTTGGAA GGCTTGAATT CTGATACCCT GTATCTAGTC 
CGTATAGCTG CAGCAACTAA AGTAGGATGG GGGGAATCTT CTGCATGGAC ATCTCACAGG 
ACACCTAAGT CTACAAGTAT AAGAGCACCG AGGTCTCCGG AGCTCCGTTT GGACCCCTTG 
AATTGCACAA CCATAACAGT CAGATGGCAG CAAGACCCTG ACGATACTGC TACCATACAG 
GGCTACAAAC TGTATTATAA AGAAGAAGGA CAGCAAGAAA GTGGGCCAGT ACTTCTGGAT 
GTGAAAGATT TTGATTATAA CATAGGAGGG TTAGATCCAA GAAAAAAATA TCATGTGAGA 
CTACTGGCCT ACAACAACAT GGATGAAGGC TACCAAGCAG ACCAAACAGT CAGCACACCA 
GTCTGTGTGT CTGCTCCTGA TCACGTAAAT CCTCCACCGC CACCACCTCA TCATCTCTAT 
GCTAAAGCAA ACAGCTCTTC TTCCATTTAC CTGCACTGGG GTCGACCAGC CTTCTCCTCT 
GCCAAGGCCA TTAACTACAC TGTCCGCTGC AATCCTGTTG GCCTGCAGAA CGCTTCCTTA 
GTGCTTTACC TACAGACGAC AGAATCTCAC ATTTTACTTC AGGATCTTGA AGCTAACAAA 
AAATATGAAT TTGCTGTGAG GCTTCATGTG GACCTTTTGT CAAGCCCCTG GAGCCCTGTG 
GTTTACCAGT CCACCCTCCC TGATGCTCCA CCAAGGCCAC CAGTGGGAGT AAAGGTGACC 
TTAATAGAAG AAAACACTGC TTTGGTCTCC TGGAAGCCCC CTGAAGAATT GGATTCAGCT 
GTGACCAGTT ATACCATCCT GTACACGTCT AGAAAGTCCT GGATTGCTGG CGAGTGGCAA 
ATTATGCATA GAGAAGGAAC AATGACCATG GCATTGTTAG AGAATCTGCT CCCTGGAAAC 
ATTTACCTTG TCAAGATTTC AGCTGCTAAT GAAGCTGGAG AGGGACCCTT CTCTAATGTT 
GTTGAGTTGG TAGTGGAGTC AAAGGAGAAT CTGCGAGTAG TCACAAGGTC AAAGCAGTTA 
GATTCTGGAC ATCGTGAAGA TTATTCCAGT TTTTACCACT TGGATCAGAA ATCTATGACT 
GGCATTGTTG TCGGAGTGTG TATTGCATTA ACTTGTATCC TGATTTGTGT CCTCATCTTG 
ATTTACCGAA GCAAAGCTAG GAAAGCATCA ATTGCTAAGA CTGCTCAGCA AGAACCCCAC 
CTTCTACCTC ATGCAACTGC ATCAGCTCCT ACTGATAGGC AGGAGAGAAA AGCAACAGAT 
CAAGTGGAGG GAAGTACCGG CCCCTTGATC CCTTCTGTGG AATCTAACCA CTTTATAGAT 
GCAAAGGGTG GCTCAAATCT TGTCATAAAT GGCTACGGGC CAGTTAAAAA TAACACCAAG 
AAATCATGGC CATTTTTCAA ACACAATAAG AATGGCTCTG TTGAACAGCC CAACAAAAGA 
ATTGTCAAAG CAATGTGCTT ATATCAGCCT GGCAATAGAG TGTTGATAGG AGAAGATTTA 
TCCCCTGCAT CTCCAGGAAA TCCTGGAACA TATAAAATGC GGAACAGCAT TGCTGATACA 
GAGCATTCTG GAAACAGTGA TGAAAGTCAT GAAACCAGTG ATTCTGGACG GTTTTCTCAT 
GAATCCACTG ATGAAATTCA CCTGTACTCA GTTGTATGTC CCACCACTCC TACATCAGAT 
TCTTTCACCA GCAATGACTG TAGACAGAAC TCTGCTTTAT ATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002940300.2
CDS	446..3913
Translation	MAACRRRLPHGVLYWVTLFSLPGILCFSDLMFVKEPQDVIILRRDSVVLDCLANGEAPITVTWMKNGVKISENERISTLHNGSLYIREAEGRKGDQSDEGFYQCLALNKFGAILSQRAHLSFASMSGFEEQPVSVQVQHGSVARFSCKITANPPAVITWEVNRTALPLAMDRITVLSKGVLQIYGVTKRDAGNYRCVATSVSNRRKSTEAVLTVVPAKEKKYFQKPVIIAPPQNITTSMHQSVTLECVATGNPKPIVSWSRRDHKPIDVFNTRVVGNGNLIISDVKPHHSGVYICRATTPGTRNFTVAMTTVTVLAPPSFTEWPASLTMPRASTARFVCQAEGNPVPKISWLKNGQKINSNGRIKMYNSKLVISHIMPEDDAVYQCMAENSQGSVLSRARLIVVMYEHRPSAPRQLRAETMSSSAIILSWERPLYNSEKIIAYSVHYMKAEGLNNEEYQVVIGNDTTHYIIDDLDPASNFTFYVVAYMPMGASQMSDHVTQSTLEDVPLRAPEISLTSRSPTDVLISWLPISAKFRRGKIIHYRLSYRMSTENTTHNVELPGSTHEYFLEGLNSDTLYLVRIAAATKVGWGESSAWTSHRTPKSTSIRAPRSPELRLDPLNCTTITVRWQQDPDDTATIQGYKLYYKEEGQQESGPVLLDVKDFDYNIGGLDPRKKYHVRLLAYNNMDEGYQADQTVSTPVCVSAPDHVNPPPPPPHHLYAKANSSSSIYLHWGRPAFSSAKAINYTVRCNPVGLQNASLVLYLQTTESHILLQDLEANKKYEFAVRLHVDLLSSPWSPVVYQSTLPDAPPRPPVGVKVTLIEENTALVSWKPPEELDSAVTSYTILYTSRKSWIAGEWQIMHREGTMTMALLENLLPGNIYLVKISAANEAGEGPFSNVVELVVESKENLRVVTRSKQLDSGHREDYSSFYHLDQKSMTGIVVGVCIALTCILICVLILIYRSKARKASIAKTAQQEPHLLPHATASAPTDRQERKATDQVEGSTGPLIPSVESNHFIDAKGGSNLVINGYGPVKNNTKKSWPFFKHNKNGSVEQPNKRIVKAMCLYQPGNRVLIGEDLSPASPGNPGTYKMRNSIADTEHSGNSDESHETSDSGRFSHESTDEIHLYSVVCPTTPTSDSFTSNDCRQNSALYE

Target ORF information:

RefSeq Version	XM_002940254.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis protogenin (prtg), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002940254.5

ORF Insert Sequence:

ATGGCGGCTT GCAGAAGGCG ACTTCCCCAC GGGGTACTCT ATTGGGTAAC CCTGTTCAGC 
CTCCCAGGGA TTTTGTGTTT TAGTGATCTG ATGTTTGTTA AAGAACCTCA AGATGTAATC 
ATTTTACGGA GAGATTCAGT GGTTTTAGAC TGCCTGGCTA ATGGAGAAGC TCCAATAACA 
GTCACGTGGA TGAAGAACGG AGTAAAGATT TCTGAGAATG AACGGATATC CACATTACAC 
AATGGATCCT TGTACATCCG TGAGGCAGAG GGCAGGAAAG GAGATCAATC TGATGAAGGA 
TTTTATCAGT GTTTGGCTTT GAACAAATTT GGTGCCATTC TAAGTCAAAG AGCTCATCTT 
TCATTTGCAA GCATGTCAGG ATTTGAAGAA CAACCTGTGT CTGTTCAGGT CCAACATGGA 
AGTGTGGCTC GATTCTCCTG TAAGATAACA GCTAATCCTC CAGCAGTCAT TACCTGGGAA 
GTAAATCGGA CTGCTCTGCC CCTTGCTATG GACAGGATAA CTGTGCTATC AAAAGGAGTT 
CTCCAGATCT ATGGAGTCAC GAAGAGGGAT GCTGGGAATT ACAGATGTGT AGCCACCAGT 
GTTTCAAACA GGCGGAAGAG CACAGAAGCT GTGCTAACTG TTGTGCCAGC TAAGGAAAAG 
AAATATTTTC AGAAACCAGT CATCATTGCT CCTCCACAGA ATATAACTAC CTCTATGCAC 
CAGTCGGTCA CTCTGGAATG TGTGGCTACA GGGAACCCAA AGCCTATTGT ATCATGGAGC 
AGGAGGGACC ATAAACCAAT TGATGTGTTT AACACACGAG TAGTTGGAAA TGGAAACCTC 
ATAATATCAG ATGTTAAGCC GCACCACTCT GGGGTCTACA TTTGTCGTGC CACCACTCCC 
GGTACACGCA ACTTTACTGT TGCAATGACA ACTGTCACAG TTCTAGCACC TCCATCCTTT 
ACTGAATGGC CAGCCAGTTT GACAATGCCA CGTGCTAGTA CTGCCAGATT TGTATGTCAA 
GCCGAAGGGA ATCCAGTTCC AAAAATTTCA TGGCTAAAGA ATGGCCAAAA AATTAATTCC 
AATGGCAGAA TTAAAATGTA CAACAGTAAA CTGGTAATTA GCCATATAAT GCCTGAAGAT 
GATGCAGTGT ATCAGTGCAT GGCAGAGAAT AGTCAAGGAT CTGTTCTTTC TCGCGCACGG 
TTGATAGTCG TTATGTATGA GCACAGACCC AGTGCTCCAC GTCAGCTTCG GGCTGAGACA 
ATGTCCAGTT CAGCCATCAT TCTCTCTTGG GAACGGCCTC TGTATAACTC AGAAAAAATC 
ATTGCTTATT CTGTGCATTA CATGAAAGCA GAAGGTTTAA ATAATGAAGA ATATCAGGTG 
GTAATTGGAA ATGACACAAC TCATTACATC ATTGATGACT TGGATCCTGC CAGCAATTTT 
ACCTTTTACG TTGTAGCATA TATGCCAATG GGGGCAAGCC AAATGTCTGA TCATGTGACA 
CAGAGCACTC TTGAAGATGT TCCTTTAAGA GCTCCAGAAA TCAGTTTAAC AAGCCGCAGT 
CCAACAGATG TCCTTATTTC TTGGCTTCCT ATTTCGGCCA AATTCCGTCG GGGAAAGATT 
ATCCATTACA GACTGTCTTA TCGAATGAGC ACTGAAAACA CCACCCATAA TGTAGAGCTC 
CCGGGAAGCA CACATGAGTA TTTCTTGGAA GGCTTGAATT CTGATACCCT GTATCTAGTC 
CGTATAGCTG CAGCAACTAA AGTAGGATGG GGGGAATCTT CTGCATGGAC ATCTCACAGG 
ACACCTAAGT CTACAAGTAT AAGAGCACCG AGGTCTCCGG AGCTCCGTTT GGACCCCTTG 
AATTGCACAA CCATAACAGT CAGATGGCAG CAAGACCCTG ACGATACTGC TACCATACAG 
GGCTACAAAC TGTATTATAA AGAAGAAGGA CAGCAAGAAA GTGGGCCAGT ACTTCTGGAT 
GTGAAAGATT TTGATTATAA CATAGGAGGG TTAGATCCAA GAAAAAAATA TCATGTGAGA 
CTACTGGCCT ACAACAACAT GGATGAAGGC TACCAAGCAG ACCAAACAGT CAGCACACCA 
GTCTGTGTGT CTGCTCCTGA TCACGTAAAT CCTCCACCGC CACCACCTCA TCATCTCTAT 
GCTAAAGCAA ACAGCTCTTC TTCCATTTAC CTGCACTGGG GTCGACCAGC CTTCTCCTCT 
GCCAAGGCCA TTAACTACAC TGTCCGCTGC AATCCTGTTG GCCTGCAGAA CGCTTCCTTA 
GTGCTTTACC TACAGACGAC AGAATCTCAC ATTTTACTTC AGGATCTTGA AGCTAACAAA 
AAATATGAAT TTGCTGTGAG GCTTCATGTG GACCTTTTGT CAAGCCCCTG GAGCCCTGTG 
GTTTACCAGT CCACCCTCCC TGATGCTCCA CCAAGGCCAC CAGTGGGAGT AAAGGTGACC 
TTAATAGAAG AAAACACTGC TTTGGTCTCC TGGAAGCCCC CTGAAGAATT GGATTCAGCT 
GTGACCAGTT ATACCATCCT GTACACGTCT AGAAAGTCCT GGATTGCTGG CGAGTGGCAA 
ATTATGCATA GAGAAGGAAC AATGACCATG GCATTGTTAG AGAATCTGCT CCCTGGAAAC 
ATTTACCTTG TCAAGATTTC AGCTGCTAAT GAAGCTGGAG AGGGACCCTT CTCTAATGTT 
GTTGAGTTGG TAGTGGAGTC AAAGGAGAAT CTGCGAGTAG TCACAAGGTC AAAGCAGTTA 
GATTCTGGAC ATCGTGAAGA TTATTCCAGT TTTTACCACT TGGATCAGAA ATCTATGACT 
GGCATTGTTG TCGGAGTGTG TATTGCATTA ACTTGTATCC TGATTTGTGT CCTCATCTTG 
ATTTACCGAA GCAAAGCTAG GAAAGCATCA ATTGCTAAGA CTGCTCAGCA AGAACCCCAC 
CTTCTACCTC ATGCAACTGC ATCAGCTCCT ACTGATAGGC AGGAGAGAAA AGCAACAGAT 
CAAGTGGAGG GAAGTACCGG CCCCTTGATC CCTTCTGTGG AATCTAACCA CTTTATAGAT 
GCAAAGGGTG GCTCAAATCT TGTCATAAAT GGCTACGGGC CAGTTAAAAA TAACACCAAG 
AAATCATGGC CATTTTTCAA ACACAATAAG AATGGCTCTG TTGAACAGCC CAACAAAAGA 
ATTGTCAAAG CAATGTGCTT ATATCAGCCT GGCAATAGAG TGTTGATAGG AGAAGATTTA 
TCCCCTGCAT CTCCAGGAAA TCCTGGAACA TATAAAATGC GGAACAGCAT TGCTGATACA 
GAGCATTCTG GAAACAGTGA TGAAAGTCAT GAAACCAGTG ATTCTGGACG GTTTTCTCAT 
GAATCCACTG ATGAAATTCA CCTGTACTCA GTTGTATGTC CCACCACTCC TACATCAGAT 
TCTTTCACCA GCAATGACTG TAGACAGAAC TCTGCTTTAT ATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prtg ( XM_004912700.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912757.1 Xenopus tropicalis protogenin (prtg), transcript variant X1, mRNA.

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CloneID	OXa07372
Clone ID Related Accession (Same CDS sequence)	XM_004912700.4 , XM_004912700.3
Accession Version	XM_004912700.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protogenin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004912700.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTT GCAGAAGGCG ACTTCCCCAC GGGGTACTCT ATTGGGTAAC CCTGTTCAGC 
CTCCCAGGGA TTTTGTGTTT TAGTGATCTG ATGTTTGTTA AAGAACCTCA AGATGTAATC 
ATTTTACGGA GAGATTCAGT GGTTTTAGAC TGCCTGGCTA ATGGAGAAGC TCCAATAACA 
GTCACGTGGA TGAAGAACGG AGTAAAGATT TCTGAGAATG AACGGATATC CACATTACAC 
AATGGATCCT TGTACATCCG TGAGGCAGAG GGCAGGAAAG GAGATCAATC TGATGAAGGA 
TTTTATCAGT GTTTGGCTTT GAACAAATTT GGTGCCATTC TAAGTCAAAG AGCTCATCTT 
TCATTTGCAA GCATGTCAGG ATTTGAAGAA CAACCTGTGT CTGTTCAGGT CCAACATGGA 
AGTGTGGCTC GATTCTCCTG TAAGATAACA GCTAATCCTC CAGCAGTCAT TACCTGGGAA 
GTAAATCGGA CTGCTCTGCC CCTTGCTATG GACAGGATAA CTGTGCTATC AAAAGGAGTT 
CTCCAGATCT ATGGAGTCAC GAAGAGGGAT GCTGGGAATT ACAGATGTGT AGCCACCAGT 
GTTTCAAACA GGCGGAAGAG CACAGAAGCT GTGCTAACTG TTGTGCCAGG AGAGAGACTG 
AATCCAAGGC ACCATCTTTC CCAAACACAA CTCACTCTGA GAGCTAAGGA AAAGAAATAT 
TTTCAGAAAC CAGTCATCAT TGCTCCTCCA CAGAATATAA CTACCTCTAT GCACCAGTCG 
GTCACTCTGG AATGTGTGGC TACAGGGAAC CCAAAGCCTA TTGTATCATG GAGCAGGAGG 
GACCATAAAC CAATTGATGT GTTTAACACA CGAGTAGTTG GAAATGGAAA CCTCATAATA 
TCAGATGTTA AGCCGCACCA CTCTGGGGTC TACATTTGTC GTGCCACCAC TCCCGGTACA 
CGCAACTTTA CTGTTGCAAT GACAACTGTC ACAGTTCTAG CACCTCCATC CTTTACTGAA 
TGGCCAGCCA GTTTGACAAT GCCACGTGCT AGTACTGCCA GATTTGTATG TCAAGCCGAA 
GGGAATCCAG TTCCAAAAAT TTCATGGCTA AAGAATGGCC AAAAAATTAA TTCCAATGGC 
AGAATTAAAA TGTACAACAG TAAACTGGTA ATTAGCCATA TAATGCCTGA AGATGATGCA 
GTGTATCAGT GCATGGCAGA GAATAGTCAA GGATCTGTTC TTTCTCGCGC ACGGTTGATA 
GTCGTTATGT ATGAGCACAG ACCCAGTGCT CCACGTCAGC TTCGGGCTGA GACAATGTCC 
AGTTCAGCCA TCATTCTCTC TTGGGAACGG CCTCTGTATA ACTCAGAAAA AATCATTGCT 
TATTCTGTGC ATTACATGAA AGCAGAAGGT TTAAATAATG AAGAATATCA GGTGGTAATT 
GGAAATGACA CAACTCATTA CATCATTGAT GACTTGGATC CTGCCAGCAA TTTTACCTTT 
TACGTTGTAG CATATATGCC AATGGGGGCA AGCCAAATGT CTGATCATGT GACACAGAGC 
ACTCTTGAAG ATGTTCCTTT AAGAGCTCCA GAAATCAGTT TAACAAGCCG CAGTCCAACA 
GATGTCCTTA TTTCTTGGCT TCCTATTTCG GCCAAATTCC GTCGGGGAAA GATTATCCAT 
TACAGACTGT CTTATCGAAT GAGCACTGAA AACACCACCC ATAATGTAGA GCTCCCGGGA 
AGCACACATG AGTATTTCTT GGAAGGCTTG AATTCTGATA CCCTGTATCT AGTCCGTATA 
GCTGCAGCAA CTAAAGTAGG ATGGGGGGAA TCTTCTGCAT GGACATCTCA CAGGACACCT 
AAGTCTACAA GTATAAGAGC ACCGAGGTCT CCGGAGCTCC GTTTGGACCC CTTGAATTGC 
ACAACCATAA CAGTCAGATG GCAGCAAGAC CCTGACGATA CTGCTACCAT ACAGGGCTAC 
AAACTGTATT ATAAAGAAGA AGGACAGCAA GAAAGTGGGC CAGTACTTCT GGATGTGAAA 
GATTTTGATT ATAACATAGG AGGGTTAGAT CCAAGAAAAA AATATCATGT GAGACTACTG 
GCCTACAACA ACATGGATGA AGGCTACCAA GCAGACCAAA CAGTCAGCAC ACCAGTCTGT 
GTGTCTGCTC CTGATCACGT AAATCCTCCA CCGCCACCAC CTCATCATCT CTATGCTAAA 
GCAAACAGCT CTTCTTCCAT TTACCTGCAC TGGGGTCGAC CAGCCTTCTC CTCTGCCAAG 
GCCATTAACT ACACTGTCCG CTGCAATCCT GTTGGCCTGC AGAACGCTTC CTTAGTGCTT 
TACCTACAGA CGACAGAATC TCACATTTTA CTTCAGGATC TTGAAGCTAA CAAAAAATAT 
GAATTTGCTG TGAGGCTTCA TGTGGACCTT TTGTCAAGCC CCTGGAGCCC TGTGGTTTAC 
CAGTCCACCC TCCCTGATGC TCCACCAAGG CCACCAGTGG GAGTAAAGGT GACCTTAATA 
GAAGAAAACA CTGCTTTGGT CTCCTGGAAG CCCCCTGAAG AATTGGATTC AGCTGTGACC 
AGTTATACCA TCCTGTACAC GTCTAGAAAG TCCTGGATTG CTGGCGAGTG GCAAATTATG 
CATAGAGAAG GAACAATGAC CATGGCATTG TTAGAGAATC TGCTCCCTGG AAACATTTAC 
CTTGTCAAGA TTTCAGCTGC TAATGAAGCT GGAGAGGGAC CCTTCTCTAA TGTTGTTGAG 
TTGGTAGTGG AGTCAAAGGA GAATCTGCGA GTAGTCACAA GGTCAAAGCA GTTAGATTCT 
GGACATCGTG AAGATTATTC CAGTTTTTAC CACTTGGATC AGAAATCTAT GACTGGCATT 
GTTGTCGGAG TGTGTATTGC ATTAACTTGT ATCCTGATTT GTGTCCTCAT CTTGATTTAC 
CGAAGCAAAG CTAGGAAAGC ATCAATTGCT AAGACTGCTC AGCAAGAACC CCACCTTCTA 
CCTCATGCAA CTGCATCAGC TCCTACTGAT AGGCAGGAGA GAAAAGCAAC AGATCAAGTG 
GAGGGAAGTA CCGGCCCCTT GATCCCTTCT GTGGAATCTA ACCACTTTAT AGATGCAAAG 
GGTGGCTCAA ATCTTGTCAT AAATGGCTAC GGGCCAGTTA AAAATAACAC CAAGAAATCA 
TGGCCATTTT TCAAACACAA TAAGAATGGC TCTGTTGAAC AGCCCAACAA AAGAATTGTC 
AAAGCAATGT GCTTATATCA GCCTGGCAAT AGAGTGTTGA TAGGAGAAGA TTTATCCCCT 
GCATCTCCAG GAAATCCTGG AACATATAAA ATGCGGAACA GCATTGCTGA TACAGAGCAT 
TCTGGAAACA GTGATGAAAG TCATGAAACC AGTGATTCTG GACGGTTTTC TCATGAATCC 
ACTGATGAAA TTCACCTGTA CTCAGTTGTA TGTCCCACCA CTCCTACATC AGATTCTTTC 
ACCAGCAATG ACTGTAGACA GAACTCTGCT TTATATGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912757.1
CDS	1661..5182
Translation	MAACRRRLPHGVLYWVTLFSLPGILCFSDLMFVKEPQDVIILRRDSVVLDCLANGEAPITVTWMKNGVKISENERISTLHNGSLYIREAEGRKGDQSDEGFYQCLALNKFGAILSQRAHLSFASMSGFEEQPVSVQVQHGSVARFSCKITANPPAVITWEVNRTALPLAMDRITVLSKGVLQIYGVTKRDAGNYRCVATSVSNRRKSTEAVLTVVPGERLNPRHHLSQTQLTLRAKEKKYFQKPVIIAPPQNITTSMHQSVTLECVATGNPKPIVSWSRRDHKPIDVFNTRVVGNGNLIISDVKPHHSGVYICRATTPGTRNFTVAMTTVTVLAPPSFTEWPASLTMPRASTARFVCQAEGNPVPKISWLKNGQKINSNGRIKMYNSKLVISHIMPEDDAVYQCMAENSQGSVLSRARLIVVMYEHRPSAPRQLRAETMSSSAIILSWERPLYNSEKIIAYSVHYMKAEGLNNEEYQVVIGNDTTHYIIDDLDPASNFTFYVVAYMPMGASQMSDHVTQSTLEDVPLRAPEISLTSRSPTDVLISWLPISAKFRRGKIIHYRLSYRMSTENTTHNVELPGSTHEYFLEGLNSDTLYLVRIAAATKVGWGESSAWTSHRTPKSTSIRAPRSPELRLDPLNCTTITVRWQQDPDDTATIQGYKLYYKEEGQQESGPVLLDVKDFDYNIGGLDPRKKYHVRLLAYNNMDEGYQADQTVSTPVCVSAPDHVNPPPPPPHHLYAKANSSSSIYLHWGRPAFSSAKAINYTVRCNPVGLQNASLVLYLQTTESHILLQDLEANKKYEFAVRLHVDLLSSPWSPVVYQSTLPDAPPRPPVGVKVTLIEENTALVSWKPPEELDSAVTSYTILYTSRKSWIAGEWQIMHREGTMTMALLENLLPGNIYLVKISAANEAGEGPFSNVVELVVESKENLRVVTRSKQLDSGHREDYSSFYHLDQKSMTGIVVGVCIALTCILICVLILIYRSKARKASIAKTAQQEPHLLPHATASAPTDRQERKATDQVEGSTGPLIPSVESNHFIDAKGGSNLVINGYGPVKNNTKKSWPFFKHNKNGSVEQPNKRIVKAMCLYQPGNRVLIGEDLSPASPGNPGTYKMRNSIADTEHSGNSDESHETSDSGRFSHESTDEIHLYSVVCPTTPTSDSFTSNDCRQNSALYE

Target ORF information:

RefSeq Version	XM_004912700.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis protogenin (prtg), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912700.3

ORF Insert Sequence:

ATGGCGGCTT GCAGAAGGCG ACTTCCCCAC GGGGTACTCT ATTGGGTAAC CCTGTTCAGC 
CTCCCAGGGA TTTTGTGTTT TAGTGATCTG ATGTTTGTTA AAGAACCTCA AGATGTAATC 
ATTTTACGGA GAGATTCAGT GGTTTTAGAC TGCCTGGCTA ATGGAGAAGC TCCAATAACA 
GTCACGTGGA TGAAGAACGG AGTAAAGATT TCTGAGAATG AACGGATATC CACATTACAC 
AATGGATCCT TGTACATCCG TGAGGCAGAG GGCAGGAAAG GAGATCAATC TGATGAAGGA 
TTTTATCAGT GTTTGGCTTT GAACAAATTT GGTGCCATTC TAAGTCAAAG AGCTCATCTT 
TCATTTGCAA GCATGTCAGG ATTTGAAGAA CAACCTGTGT CTGTTCAGGT CCAACATGGA 
AGTGTGGCTC GATTCTCCTG TAAGATAACA GCTAATCCTC CAGCAGTCAT TACCTGGGAA 
GTAAATCGGA CTGCTCTGCC CCTTGCTATG GACAGGATAA CTGTGCTATC AAAAGGAGTT 
CTCCAGATCT ATGGAGTCAC GAAGAGGGAT GCTGGGAATT ACAGATGTGT AGCCACCAGT 
GTTTCAAACA GGCGGAAGAG CACAGAAGCT GTGCTAACTG TTGTGCCAGG AGAGAGACTG 
AATCCAAGGC ACCATCTTTC CCAAACACAA CTCACTCTGA GAGCTAAGGA AAAGAAATAT 
TTTCAGAAAC CAGTCATCAT TGCTCCTCCA CAGAATATAA CTACCTCTAT GCACCAGTCG 
GTCACTCTGG AATGTGTGGC TACAGGGAAC CCAAAGCCTA TTGTATCATG GAGCAGGAGG 
GACCATAAAC CAATTGATGT GTTTAACACA CGAGTAGTTG GAAATGGAAA CCTCATAATA 
TCAGATGTTA AGCCGCACCA CTCTGGGGTC TACATTTGTC GTGCCACCAC TCCCGGTACA 
CGCAACTTTA CTGTTGCAAT GACAACTGTC ACAGTTCTAG CACCTCCATC CTTTACTGAA 
TGGCCAGCCA GTTTGACAAT GCCACGTGCT AGTACTGCCA GATTTGTATG TCAAGCCGAA 
GGGAATCCAG TTCCAAAAAT TTCATGGCTA AAGAATGGCC AAAAAATTAA TTCCAATGGC 
AGAATTAAAA TGTACAACAG TAAACTGGTA ATTAGCCATA TAATGCCTGA AGATGATGCA 
GTGTATCAGT GCATGGCAGA GAATAGTCAA GGATCTGTTC TTTCTCGCGC ACGGTTGATA 
GTCGTTATGT ATGAGCACAG ACCCAGTGCT CCACGTCAGC TTCGGGCTGA GACAATGTCC 
AGTTCAGCCA TCATTCTCTC TTGGGAACGG CCTCTGTATA ACTCAGAAAA AATCATTGCT 
TATTCTGTGC ATTACATGAA AGCAGAAGGT TTAAATAATG AAGAATATCA GGTGGTAATT 
GGAAATGACA CAACTCATTA CATCATTGAT GACTTGGATC CTGCCAGCAA TTTTACCTTT 
TACGTTGTAG CATATATGCC AATGGGGGCA AGCCAAATGT CTGATCATGT GACACAGAGC 
ACTCTTGAAG ATGTTCCTTT AAGAGCTCCA GAAATCAGTT TAACAAGCCG CAGTCCAACA 
GATGTCCTTA TTTCTTGGCT TCCTATTTCG GCCAAATTCC GTCGGGGAAA GATTATCCAT 
TACAGACTGT CTTATCGAAT GAGCACTGAA AACACCACCC ATAATGTAGA GCTCCCGGGA 
AGCACACATG AGTATTTCTT GGAAGGCTTG AATTCTGATA CCCTGTATCT AGTCCGTATA 
GCTGCAGCAA CTAAAGTAGG ATGGGGGGAA TCTTCTGCAT GGACATCTCA CAGGACACCT 
AAGTCTACAA GTATAAGAGC ACCGAGGTCT CCGGAGCTCC GTTTGGACCC CTTGAATTGC 
ACAACCATAA CAGTCAGATG GCAGCAAGAC CCTGACGATA CTGCTACCAT ACAGGGCTAC 
AAACTGTATT ATAAAGAAGA AGGACAGCAA GAAAGTGGGC CAGTACTTCT GGATGTGAAA 
GATTTTGATT ATAACATAGG AGGGTTAGAT CCAAGAAAAA AATATCATGT GAGACTACTG 
GCCTACAACA ACATGGATGA AGGCTACCAA GCAGACCAAA CAGTCAGCAC ACCAGTCTGT 
GTGTCTGCTC CTGATCACGT AAATCCTCCA CCGCCACCAC CTCATCATCT CTATGCTAAA 
GCAAACAGCT CTTCTTCCAT TTACCTGCAC TGGGGTCGAC CAGCCTTCTC CTCTGCCAAG 
GCCATTAACT ACACTGTCCG CTGCAATCCT GTTGGCCTGC AGAACGCTTC CTTAGTGCTT 
TACCTACAGA CGACAGAATC TCACATTTTA CTTCAGGATC TTGAAGCTAA CAAAAAATAT 
GAATTTGCTG TGAGGCTTCA TGTGGACCTT TTGTCAAGCC CCTGGAGCCC TGTGGTTTAC 
CAGTCCACCC TCCCTGATGC TCCACCAAGG CCACCAGTGG GAGTAAAGGT GACCTTAATA 
GAAGAAAACA CTGCTTTGGT CTCCTGGAAG CCCCCTGAAG AATTGGATTC AGCTGTGACC 
AGTTATACCA TCCTGTACAC GTCTAGAAAG TCCTGGATTG CTGGCGAGTG GCAAATTATG 
CATAGAGAAG GAACAATGAC CATGGCATTG TTAGAGAATC TGCTCCCTGG AAACATTTAC 
CTTGTCAAGA TTTCAGCTGC TAATGAAGCT GGAGAGGGAC CCTTCTCTAA TGTTGTTGAG 
TTGGTAGTGG AGTCAAAGGA GAATCTGCGA GTAGTCACAA GGTCAAAGCA GTTAGATTCT 
GGACATCGTG AAGATTATTC CAGTTTTTAC CACTTGGATC AGAAATCTAT GACTGGCATT 
GTTGTCGGAG TGTGTATTGC ATTAACTTGT ATCCTGATTT GTGTCCTCAT CTTGATTTAC 
CGAAGCAAAG CTAGGAAAGC ATCAATTGCT AAGACTGCTC AGCAAGAACC CCACCTTCTA 
CCTCATGCAA CTGCATCAGC TCCTACTGAT AGGCAGGAGA GAAAAGCAAC AGATCAAGTG 
GAGGGAAGTA CCGGCCCCTT GATCCCTTCT GTGGAATCTA ACCACTTTAT AGATGCAAAG 
GGTGGCTCAA ATCTTGTCAT AAATGGCTAC GGGCCAGTTA AAAATAACAC CAAGAAATCA 
TGGCCATTTT TCAAACACAA TAAGAATGGC TCTGTTGAAC AGCCCAACAA AAGAATTGTC 
AAAGCAATGT GCTTATATCA GCCTGGCAAT AGAGTGTTGA TAGGAGAAGA TTTATCCCCT 
GCATCTCCAG GAAATCCTGG AACATATAAA ATGCGGAACA GCATTGCTGA TACAGAGCAT 
TCTGGAAACA GTGATGAAAG TCATGAAACC AGTGATTCTG GACGGTTTTC TCATGAATCC 
ACTGATGAAA TTCACCTGTA CTCAGTTGTA TGTCCCACCA CTCCTACATC AGATTCTTTC 
ACCAGCAATG ACTGTAGACA GAACTCTGCT TTATATGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prtg ( XM_002940254.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002940300.2 Xenopus tropicalis protogenin (prtg), transcript variant X2, mRNA.

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CloneID	OXa07373
Clone ID Related Accession (Same CDS sequence)	XM_002940254.4 , XM_002940254.5
Accession Version	XM_002940254.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protogenin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002940254.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTT GCAGAAGGCG ACTTCCCCAC GGGGTACTCT ATTGGGTAAC CCTGTTCAGC 
CTCCCAGGGA TTTTGTGTTT TAGTGATCTG ATGTTTGTTA AAGAACCTCA AGATGTAATC 
ATTTTACGGA GAGATTCAGT GGTTTTAGAC TGCCTGGCTA ATGGAGAAGC TCCAATAACA 
GTCACGTGGA TGAAGAACGG AGTAAAGATT TCTGAGAATG AACGGATATC CACATTACAC 
AATGGATCCT TGTACATCCG TGAGGCAGAG GGCAGGAAAG GAGATCAATC TGATGAAGGA 
TTTTATCAGT GTTTGGCTTT GAACAAATTT GGTGCCATTC TAAGTCAAAG AGCTCATCTT 
TCATTTGCAA GCATGTCAGG ATTTGAAGAA CAACCTGTGT CTGTTCAGGT CCAACATGGA 
AGTGTGGCTC GATTCTCCTG TAAGATAACA GCTAATCCTC CAGCAGTCAT TACCTGGGAA 
GTAAATCGGA CTGCTCTGCC CCTTGCTATG GACAGGATAA CTGTGCTATC AAAAGGAGTT 
CTCCAGATCT ATGGAGTCAC GAAGAGGGAT GCTGGGAATT ACAGATGTGT AGCCACCAGT 
GTTTCAAACA GGCGGAAGAG CACAGAAGCT GTGCTAACTG TTGTGCCAGC TAAGGAAAAG 
AAATATTTTC AGAAACCAGT CATCATTGCT CCTCCACAGA ATATAACTAC CTCTATGCAC 
CAGTCGGTCA CTCTGGAATG TGTGGCTACA GGGAACCCAA AGCCTATTGT ATCATGGAGC 
AGGAGGGACC ATAAACCAAT TGATGTGTTT AACACACGAG TAGTTGGAAA TGGAAACCTC 
ATAATATCAG ATGTTAAGCC GCACCACTCT GGGGTCTACA TTTGTCGTGC CACCACTCCC 
GGTACACGCA ACTTTACTGT TGCAATGACA ACTGTCACAG TTCTAGCACC TCCATCCTTT 
ACTGAATGGC CAGCCAGTTT GACAATGCCA CGTGCTAGTA CTGCCAGATT TGTATGTCAA 
GCCGAAGGGA ATCCAGTTCC AAAAATTTCA TGGCTAAAGA ATGGCCAAAA AATTAATTCC 
AATGGCAGAA TTAAAATGTA CAACAGTAAA CTGGTAATTA GCCATATAAT GCCTGAAGAT 
GATGCAGTGT ATCAGTGCAT GGCAGAGAAT AGTCAAGGAT CTGTTCTTTC TCGCGCACGG 
TTGATAGTCG TTATGTATGA GCACAGACCC AGTGCTCCAC GTCAGCTTCG GGCTGAGACA 
ATGTCCAGTT CAGCCATCAT TCTCTCTTGG GAACGGCCTC TGTATAACTC AGAAAAAATC 
ATTGCTTATT CTGTGCATTA CATGAAAGCA GAAGGTTTAA ATAATGAAGA ATATCAGGTG 
GTAATTGGAA ATGACACAAC TCATTACATC ATTGATGACT TGGATCCTGC CAGCAATTTT 
ACCTTTTACG TTGTAGCATA TATGCCAATG GGGGCAAGCC AAATGTCTGA TCATGTGACA 
CAGAGCACTC TTGAAGATGT TCCTTTAAGA GCTCCAGAAA TCAGTTTAAC AAGCCGCAGT 
CCAACAGATG TCCTTATTTC TTGGCTTCCT ATTTCGGCCA AATTCCGTCG GGGAAAGATT 
ATCCATTACA GACTGTCTTA TCGAATGAGC ACTGAAAACA CCACCCATAA TGTAGAGCTC 
CCGGGAAGCA CACATGAGTA TTTCTTGGAA GGCTTGAATT CTGATACCCT GTATCTAGTC 
CGTATAGCTG CAGCAACTAA AGTAGGATGG GGGGAATCTT CTGCATGGAC ATCTCACAGG 
ACACCTAAGT CTACAAGTAT AAGAGCACCG AGGTCTCCGG AGCTCCGTTT GGACCCCTTG 
AATTGCACAA CCATAACAGT CAGATGGCAG CAAGACCCTG ACGATACTGC TACCATACAG 
GGCTACAAAC TGTATTATAA AGAAGAAGGA CAGCAAGAAA GTGGGCCAGT ACTTCTGGAT 
GTGAAAGATT TTGATTATAA CATAGGAGGG TTAGATCCAA GAAAAAAATA TCATGTGAGA 
CTACTGGCCT ACAACAACAT GGATGAAGGC TACCAAGCAG ACCAAACAGT CAGCACACCA 
GTCTGTGTGT CTGCTCCTGA TCACGTAAAT CCTCCACCGC CACCACCTCA TCATCTCTAT 
GCTAAAGCAA ACAGCTCTTC TTCCATTTAC CTGCACTGGG GTCGACCAGC CTTCTCCTCT 
GCCAAGGCCA TTAACTACAC TGTCCGCTGC AATCCTGTTG GCCTGCAGAA CGCTTCCTTA 
GTGCTTTACC TACAGACGAC AGAATCTCAC ATTTTACTTC AGGATCTTGA AGCTAACAAA 
AAATATGAAT TTGCTGTGAG GCTTCATGTG GACCTTTTGT CAAGCCCCTG GAGCCCTGTG 
GTTTACCAGT CCACCCTCCC TGATGCTCCA CCAAGGCCAC CAGTGGGAGT AAAGGTGACC 
TTAATAGAAG AAAACACTGC TTTGGTCTCC TGGAAGCCCC CTGAAGAATT GGATTCAGCT 
GTGACCAGTT ATACCATCCT GTACACGTCT AGAAAGTCCT GGATTGCTGG CGAGTGGCAA 
ATTATGCATA GAGAAGGAAC AATGACCATG GCATTGTTAG AGAATCTGCT CCCTGGAAAC 
ATTTACCTTG TCAAGATTTC AGCTGCTAAT GAAGCTGGAG AGGGACCCTT CTCTAATGTT 
GTTGAGTTGG TAGTGGAGTC AAAGGAGAAT CTGCGAGTAG TCACAAGGTC AAAGCAGTTA 
GATTCTGGAC ATCGTGAAGA TTATTCCAGT TTTTACCACT TGGATCAGAA ATCTATGACT 
GGCATTGTTG TCGGAGTGTG TATTGCATTA ACTTGTATCC TGATTTGTGT CCTCATCTTG 
ATTTACCGAA GCAAAGCTAG GAAAGCATCA ATTGCTAAGA CTGCTCAGCA AGAACCCCAC 
CTTCTACCTC ATGCAACTGC ATCAGCTCCT ACTGATAGGC AGGAGAGAAA AGCAACAGAT 
CAAGTGGAGG GAAGTACCGG CCCCTTGATC CCTTCTGTGG AATCTAACCA CTTTATAGAT 
GCAAAGGGTG GCTCAAATCT TGTCATAAAT GGCTACGGGC CAGTTAAAAA TAACACCAAG 
AAATCATGGC CATTTTTCAA ACACAATAAG AATGGCTCTG TTGAACAGCC CAACAAAAGA 
ATTGTCAAAG CAATGTGCTT ATATCAGCCT GGCAATAGAG TGTTGATAGG AGAAGATTTA 
TCCCCTGCAT CTCCAGGAAA TCCTGGAACA TATAAAATGC GGAACAGCAT TGCTGATACA 
GAGCATTCTG GAAACAGTGA TGAAAGTCAT GAAACCAGTG ATTCTGGACG GTTTTCTCAT 
GAATCCACTG ATGAAATTCA CCTGTACTCA GTTGTATGTC CCACCACTCC TACATCAGAT 
TCTTTCACCA GCAATGACTG TAGACAGAAC TCTGCTTTAT ATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002940300.2
CDS	1661..5128
Translation	MAACRRRLPHGVLYWVTLFSLPGILCFSDLMFVKEPQDVIILRRDSVVLDCLANGEAPITVTWMKNGVKISENERISTLHNGSLYIREAEGRKGDQSDEGFYQCLALNKFGAILSQRAHLSFASMSGFEEQPVSVQVQHGSVARFSCKITANPPAVITWEVNRTALPLAMDRITVLSKGVLQIYGVTKRDAGNYRCVATSVSNRRKSTEAVLTVVPAKEKKYFQKPVIIAPPQNITTSMHQSVTLECVATGNPKPIVSWSRRDHKPIDVFNTRVVGNGNLIISDVKPHHSGVYICRATTPGTRNFTVAMTTVTVLAPPSFTEWPASLTMPRASTARFVCQAEGNPVPKISWLKNGQKINSNGRIKMYNSKLVISHIMPEDDAVYQCMAENSQGSVLSRARLIVVMYEHRPSAPRQLRAETMSSSAIILSWERPLYNSEKIIAYSVHYMKAEGLNNEEYQVVIGNDTTHYIIDDLDPASNFTFYVVAYMPMGASQMSDHVTQSTLEDVPLRAPEISLTSRSPTDVLISWLPISAKFRRGKIIHYRLSYRMSTENTTHNVELPGSTHEYFLEGLNSDTLYLVRIAAATKVGWGESSAWTSHRTPKSTSIRAPRSPELRLDPLNCTTITVRWQQDPDDTATIQGYKLYYKEEGQQESGPVLLDVKDFDYNIGGLDPRKKYHVRLLAYNNMDEGYQADQTVSTPVCVSAPDHVNPPPPPPHHLYAKANSSSSIYLHWGRPAFSSAKAINYTVRCNPVGLQNASLVLYLQTTESHILLQDLEANKKYEFAVRLHVDLLSSPWSPVVYQSTLPDAPPRPPVGVKVTLIEENTALVSWKPPEELDSAVTSYTILYTSRKSWIAGEWQIMHREGTMTMALLENLLPGNIYLVKISAANEAGEGPFSNVVELVVESKENLRVVTRSKQLDSGHREDYSSFYHLDQKSMTGIVVGVCIALTCILICVLILIYRSKARKASIAKTAQQEPHLLPHATASAPTDRQERKATDQVEGSTGPLIPSVESNHFIDAKGGSNLVINGYGPVKNNTKKSWPFFKHNKNGSVEQPNKRIVKAMCLYQPGNRVLIGEDLSPASPGNPGTYKMRNSIADTEHSGNSDESHETSDSGRFSHESTDEIHLYSVVCPTTPTSDSFTSNDCRQNSALYE

Target ORF information:

RefSeq Version	XM_002940254.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis protogenin (prtg), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002940254.4

ORF Insert Sequence:

ATGGCGGCTT GCAGAAGGCG ACTTCCCCAC GGGGTACTCT ATTGGGTAAC CCTGTTCAGC 
CTCCCAGGGA TTTTGTGTTT TAGTGATCTG ATGTTTGTTA AAGAACCTCA AGATGTAATC 
ATTTTACGGA GAGATTCAGT GGTTTTAGAC TGCCTGGCTA ATGGAGAAGC TCCAATAACA 
GTCACGTGGA TGAAGAACGG AGTAAAGATT TCTGAGAATG AACGGATATC CACATTACAC 
AATGGATCCT TGTACATCCG TGAGGCAGAG GGCAGGAAAG GAGATCAATC TGATGAAGGA 
TTTTATCAGT GTTTGGCTTT GAACAAATTT GGTGCCATTC TAAGTCAAAG AGCTCATCTT 
TCATTTGCAA GCATGTCAGG ATTTGAAGAA CAACCTGTGT CTGTTCAGGT CCAACATGGA 
AGTGTGGCTC GATTCTCCTG TAAGATAACA GCTAATCCTC CAGCAGTCAT TACCTGGGAA 
GTAAATCGGA CTGCTCTGCC CCTTGCTATG GACAGGATAA CTGTGCTATC AAAAGGAGTT 
CTCCAGATCT ATGGAGTCAC GAAGAGGGAT GCTGGGAATT ACAGATGTGT AGCCACCAGT 
GTTTCAAACA GGCGGAAGAG CACAGAAGCT GTGCTAACTG TTGTGCCAGC TAAGGAAAAG 
AAATATTTTC AGAAACCAGT CATCATTGCT CCTCCACAGA ATATAACTAC CTCTATGCAC 
CAGTCGGTCA CTCTGGAATG TGTGGCTACA GGGAACCCAA AGCCTATTGT ATCATGGAGC 
AGGAGGGACC ATAAACCAAT TGATGTGTTT AACACACGAG TAGTTGGAAA TGGAAACCTC 
ATAATATCAG ATGTTAAGCC GCACCACTCT GGGGTCTACA TTTGTCGTGC CACCACTCCC 
GGTACACGCA ACTTTACTGT TGCAATGACA ACTGTCACAG TTCTAGCACC TCCATCCTTT 
ACTGAATGGC CAGCCAGTTT GACAATGCCA CGTGCTAGTA CTGCCAGATT TGTATGTCAA 
GCCGAAGGGA ATCCAGTTCC AAAAATTTCA TGGCTAAAGA ATGGCCAAAA AATTAATTCC 
AATGGCAGAA TTAAAATGTA CAACAGTAAA CTGGTAATTA GCCATATAAT GCCTGAAGAT 
GATGCAGTGT ATCAGTGCAT GGCAGAGAAT AGTCAAGGAT CTGTTCTTTC TCGCGCACGG 
TTGATAGTCG TTATGTATGA GCACAGACCC AGTGCTCCAC GTCAGCTTCG GGCTGAGACA 
ATGTCCAGTT CAGCCATCAT TCTCTCTTGG GAACGGCCTC TGTATAACTC AGAAAAAATC 
ATTGCTTATT CTGTGCATTA CATGAAAGCA GAAGGTTTAA ATAATGAAGA ATATCAGGTG 
GTAATTGGAA ATGACACAAC TCATTACATC ATTGATGACT TGGATCCTGC CAGCAATTTT 
ACCTTTTACG TTGTAGCATA TATGCCAATG GGGGCAAGCC AAATGTCTGA TCATGTGACA 
CAGAGCACTC TTGAAGATGT TCCTTTAAGA GCTCCAGAAA TCAGTTTAAC AAGCCGCAGT 
CCAACAGATG TCCTTATTTC TTGGCTTCCT ATTTCGGCCA AATTCCGTCG GGGAAAGATT 
ATCCATTACA GACTGTCTTA TCGAATGAGC ACTGAAAACA CCACCCATAA TGTAGAGCTC 
CCGGGAAGCA CACATGAGTA TTTCTTGGAA GGCTTGAATT CTGATACCCT GTATCTAGTC 
CGTATAGCTG CAGCAACTAA AGTAGGATGG GGGGAATCTT CTGCATGGAC ATCTCACAGG 
ACACCTAAGT CTACAAGTAT AAGAGCACCG AGGTCTCCGG AGCTCCGTTT GGACCCCTTG 
AATTGCACAA CCATAACAGT CAGATGGCAG CAAGACCCTG ACGATACTGC TACCATACAG 
GGCTACAAAC TGTATTATAA AGAAGAAGGA CAGCAAGAAA GTGGGCCAGT ACTTCTGGAT 
GTGAAAGATT TTGATTATAA CATAGGAGGG TTAGATCCAA GAAAAAAATA TCATGTGAGA 
CTACTGGCCT ACAACAACAT GGATGAAGGC TACCAAGCAG ACCAAACAGT CAGCACACCA 
GTCTGTGTGT CTGCTCCTGA TCACGTAAAT CCTCCACCGC CACCACCTCA TCATCTCTAT 
GCTAAAGCAA ACAGCTCTTC TTCCATTTAC CTGCACTGGG GTCGACCAGC CTTCTCCTCT 
GCCAAGGCCA TTAACTACAC TGTCCGCTGC AATCCTGTTG GCCTGCAGAA CGCTTCCTTA 
GTGCTTTACC TACAGACGAC AGAATCTCAC ATTTTACTTC AGGATCTTGA AGCTAACAAA 
AAATATGAAT TTGCTGTGAG GCTTCATGTG GACCTTTTGT CAAGCCCCTG GAGCCCTGTG 
GTTTACCAGT CCACCCTCCC TGATGCTCCA CCAAGGCCAC CAGTGGGAGT AAAGGTGACC 
TTAATAGAAG AAAACACTGC TTTGGTCTCC TGGAAGCCCC CTGAAGAATT GGATTCAGCT 
GTGACCAGTT ATACCATCCT GTACACGTCT AGAAAGTCCT GGATTGCTGG CGAGTGGCAA 
ATTATGCATA GAGAAGGAAC AATGACCATG GCATTGTTAG AGAATCTGCT CCCTGGAAAC 
ATTTACCTTG TCAAGATTTC AGCTGCTAAT GAAGCTGGAG AGGGACCCTT CTCTAATGTT 
GTTGAGTTGG TAGTGGAGTC AAAGGAGAAT CTGCGAGTAG TCACAAGGTC AAAGCAGTTA 
GATTCTGGAC ATCGTGAAGA TTATTCCAGT TTTTACCACT TGGATCAGAA ATCTATGACT 
GGCATTGTTG TCGGAGTGTG TATTGCATTA ACTTGTATCC TGATTTGTGT CCTCATCTTG 
ATTTACCGAA GCAAAGCTAG GAAAGCATCA ATTGCTAAGA CTGCTCAGCA AGAACCCCAC 
CTTCTACCTC ATGCAACTGC ATCAGCTCCT ACTGATAGGC AGGAGAGAAA AGCAACAGAT 
CAAGTGGAGG GAAGTACCGG CCCCTTGATC CCTTCTGTGG AATCTAACCA CTTTATAGAT 
GCAAAGGGTG GCTCAAATCT TGTCATAAAT GGCTACGGGC CAGTTAAAAA TAACACCAAG 
AAATCATGGC CATTTTTCAA ACACAATAAG AATGGCTCTG TTGAACAGCC CAACAAAAGA 
ATTGTCAAAG CAATGTGCTT ATATCAGCCT GGCAATAGAG TGTTGATAGG AGAAGATTTA 
TCCCCTGCAT CTCCAGGAAA TCCTGGAACA TATAAAATGC GGAACAGCAT TGCTGATACA 
GAGCATTCTG GAAACAGTGA TGAAAGTCAT GAAACCAGTG ATTCTGGACG GTTTTCTCAT 
GAATCCACTG ATGAAATTCA CCTGTACTCA GTTGTATGTC CCACCACTCC TACATCAGAT 
TCTTTCACCA GCAATGACTG TAGACAGAAC TCTGCTTTAT ATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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prtg cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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