diaph1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	diaph1
Entrez Gene ID	733754
Full Name	diaphanous related formin 1
Synonyms	dfna1,dia1,diap1,drf1,lfhl1
General protein information	Preferred Names diaphanous related formin 1 Names protein diaphanous homolog 1 diaphanous homolog 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012959693.2	XP_012815147.1	protein diaphanous homolog 1 isoform X1
XM_012959694.2	XP_012815148.1	protein diaphanous homolog 1 isoform X2
XM_012959694.3	XP_012815148.2	protein diaphanous homolog 1 isoform X2
XM_031898781.1	XP_031754641.1	protein diaphanous homolog 1 isoform X3
XM_012959695.2	XP_012815149.1	protein diaphanous homolog 1 isoform X3
XM_031898780.1	XP_031754640.1	protein diaphanous homolog 1 isoform X1
XM_012959695.3	XP_012815149.2	protein diaphanous homolog 1 isoform X4

Pathways from BioSystems

Homologies

Homo sapiens (human)	DIAPH1	NP_001073280.1
	DIAPH1	XP_001089232.2
Rattus norvegicus (Norway rat)	Diaph1	NP_001100863.2
Mus musculus (house mouse)	Diap1	NP_031884.1
Pan troglodytes (chimpanzee)	DIAPH1	XP_003310971.1
Drosophila melanogaster (fruit fly)	dia	NP_001246113.1
Canis lupus familiaris (dog)	DIAPH1	XP_005617363.1
Xenopus tropicalis (tropical clawed frog)	diaph1	XP_002940036.2

Gene Ontology

The following diaph1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the diaph1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa54437	XM_031898780.1 Latest version!	Xenopus tropicalis diaphanous related formin 1 (diaph1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa54438	XM_012959694.3 Latest version!	Xenopus tropicalis diaphanous related formin 1 (diaph1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa54439	XM_031898781.1 Latest version!	Xenopus tropicalis diaphanous related formin 1 (diaph1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa54440	XM_012959695.3 Latest version!	Xenopus tropicalis diaphanous related formin 1 (diaph1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa28573	XM_012959693.2 Latest version!	Xenopus tropicalis diaphanous related formin 1 (diaph1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OXa28574	XM_012959694.2 Latest version!	Xenopus tropicalis diaphanous related formin 1 (diaph1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OXa28575	XM_012959695.2 Latest version!	Xenopus tropicalis diaphanous related formin 1 (diaph1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

diaph1 ( XM_031898780.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031754640.1 Xenopus tropicalis diaphanous related formin 1 (diaph1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa54437
Clone ID Related Accession (Same CDS sequence)	XM_031898780.1
Accession Version	XM_031898780.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3837bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein diaphanous homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACCGC CAGCCGGTGG AGGCGGATAT GGAGGGGGAC CCCCGCCACC TTCCAAGGGC 
AACAAGGAGA AGAAGAAGCC GAAGGGCCAA GATGAAGGTG GAGATGGCAA GTCCAAGAAA 
TTTACCTTTA AGCGGCTGAT GGTAGATGAG CTTGAACGAT TTACCAGTAT ACGGATGAAG 
AAAGAAAAGG AAAAGCCTTA TCCATCACAC AGAAACTCCA CCAACTTCCC AGATAACTCA 
AACCCCCTCC AAACCTTGCA GGAACTCGAA GATGATGTTG TGCTTCAGCT GTTTGAAAAA 
ATGCTGGATG ATATGAACCT CAATCCTGAG AAGCAGCAAC CGCTACGAGA AAAGGATATA 
ATGATAAAAA GAGAGATGGT TTCCCAATAT CTTCACACAT CCAAAGCTGG CATGAGCAAA 
AAGGAGAGTT CTCGTTCTGC ATTAATGTAC ATTCAAGACT TAAAATCCGG ACTAGGAGAC 
AATTCCCTCC TTTTGTGCTT AGAGTCACTA AGAGTATCTT TAAATAACAA TCCTGTCAGC 
TGGGTGCAGA ATTTTGGTGC AGAGGGTCTT TCGTGCCTTT TAGAAATTCT CAAAAGAATA 
CAGGATGAGC AAGCATCCTT GCGGGATGTT AGAAGCGAGC ATGAGATCAT CAGATGTCTG 
AAAGCTTTTA TGAATAACAA TTTTGGAATT AAGGCAATGC TGGGCACAGA AGATGGCATT 
TTGCTGTTAG CGAGAGCAGT AGATCGATCT GTCCCTGCAA TGATGATTGA TTCCTTGAAG 
CTGCTTTCTG CCCTGTGCAT CCTGCCACAA CCAGAGGACA TGCATGAGCG TGTTCTAGAA 
GCCCTTACGG AGAGAGCAGA GATGGAGGAA ATGGAACGAT TTAAACCTCT TCTGGATGGG 
TTAAACATTG CAAGTCCTGT GGCCTTGAAG GTGAGCTGTA TGCAGCTAAT CAATGCAGTG 
ATCAGCCAAG GAGAAGAGCT TGATTACAGA GTTCACATCC GAAGTGAATT GATGCGCTCG 
GGACTGGCCA AACTCCTAAA GGAACTACGT AATACAGAAA ATGAAGACCT GAAGGTACAA 
CTTGGTATTT TTGATGAGCA TTCTGAACTT GATTCTGATG ACTTGCGAGG ACGTTTGGAC 
GACATCCGCA TTGAAATGGA TGACTTCAGT GACGTCTTCC AAGTTCTTCT GAACACTGTC 
AAGGACTCCT CTGCAGAACC ATTATTTCTT TCCATCCTGC AGCATCTTCT TCTAATTAGA 
AATGATTATG ATGCCAGGCC GCAGTACTAT AAACTGATAG ATGAAGTTAT ATCACAGATA 
ATCCTTCAAA AAAATGGGGC TGATCCAGAT TTCAAATGCA AGCATGTAAA CATAGAAATC 
GAAAATCTGA TAGATCATTT TATCGACAAA AAGAAAGTAG AAACAAGCGA GTCAAAAGCA 
CGGGATCTGG TGAAAAAGTT GGACCTAGAG CTTACCGCAC GCCACGAACT CCAGGTGGAA 
ATGAAAGCCA AAGAGAGCGA CTTTGAGCTG AAAATAACAG AGCTGCAGGG GGAGAAGGAA 
TCTTTAGATG CAGAGAAGCA AGAAATCACT GCTGACAAGC AAAAAATGGA GACAGAACTC 
TCCCAACTTG TAGAGCAGGT TGAAAAATTG TCAAAGGAAC TTGAAGCTAC CAAAAAAGAA 
ATGGCATCAA TTTCCACATC TGCTGTTTTA ATCCCTGTAC CTTCTGCCTC TGGAAATGTG 
CCACCTCCAC CACCCCCTCC TCCTCCACTT CCAGGTGCAA CTGGTATTCC TCCACCTCCC 
CCACTTCCAG GTGGCTCTGG TATTCCTCCA CCACCCCCAC TTCCAGGTGG CTCTGGTATT 
GCTCCACCAC TTCCGGCTGG CTTTGGTATT CCTCCACCTC CCCCACTTCC GGGTGGCTTT 
GGTATTCCTC CACCTCCACC ACTTCCCGGT GGCTCTGGTA TTCCTCCACC TCCCCCACTT 
CCGGGTGGCT CTGGTATTCC TCCACCTCCC CCACTTCCGG GTGGCTCTGG TATTCCTCCA 
CCTCCCCCAC TTCCGGGTGG CTCTGGTATT CCTCCCCCAC CACCATTTCC GGGTGGCCCT 
GGAATTCCTC CCCCACCGCC ATTTCCAGGA GGCTTTGGAA TTCCTCCTCC TCCTCCTCCA 
TTTCCAGGTG GCCCTGGAAT TCCTCCTCCT CCTCCATTCC CTGGTGGCCC TGGGGTTCCA 
CCTCCACCTC CAGCATTTGG AGGTGGTGTT CCTCCACCAC CAGGACTTGG AGGCTGGGGG 
GCACCACCAC CCTTACCATT TGGCTTGGCT CCTAAGAAGA ATTATAAGCC AGAAGTGCAA 
CTGAAGCGAC CCAACTGGTC AAAGATCACT CCTGCTGAGC TTTCAGAGAA ATCGTTTTGG 
ACTCAGACAA AAGAAGATCG TTTTGAAAAA GAAGAGCTGT TTGCAAAAAT AACCCTCACT 
TTTTCTTCAC AGATCAAGTC TTCTAAAGCA AAAAAAGCAG AAGATGGTGA TGACAAAAAA 
GCAGCCCAGA AGAAAAAGAC AAAGGAGCTC AAAGTCTTGG ATTCCAAGAC AGCCCAAAAC 
CTATCAATCT TCCTTGGGTC GTTCCGCATG CCATACGAAG AGATCAAAAA TGTGATCCTA 
CAGGTCAATG AGGAAGTGCT GACTGAATCT ATGGTTTCAA ATCTGATCAA GCAGTTACCA 
GAACCAGATC AGTTACAGAT GTTAACAGAG TTCAAGAATG AGTACAAGGA TTTGGCAGAA 
CCAGAGCAGT TTGGAGTAGT GATGTGCAGT GTCCCCCGTT TGCGGCCACG ACTAAATGCC 
ATTCTCTTTA AACTTCTTTT TAATGAGCAA ATTGAAAATA TCAAGCCAGA CATTGTGTCA 
GTTACAGCTG CTTGCGAAGA AGTGCAAAAG TGCCAAAGCT TTGCTAGTCT CCTGGAAATC 
ACACTGCTGG TTGGGAACTT CATGAACGCA GGATCAAGGA ACGCTGATGC TTATGGCTTT 
GACATTAGCT TTCTCTGCAA GTTGAAAGAT ACCAAATCAG CGGACCAGAA GACCACACTG 
CTGCATTTCC TTGTTGAGAC TTGTGAGAAT GACTACCCTG ATGTACTGAA GTTTCCAGAT 
GAGATGGTTC ATGTAGAAAA GGCCAGTAGA GTGTCAGCTG AAACATTGCA AAAGAACCTG 
GATCAAATGA AAAAGCAGAT TGCAGACCTT CAAAGGGACA TTGATAGCTT CCCACCAACA 
GAAGATGAGA AGGATAAGTT TGTAGAAAAA ATGACCATAT TTGTCAAAGA AGCTCAAGAA 
CAGTATACTA AGCTTTGCAT GATGCACGAA AACATGCTGT CATTGTTCCA AGATTTGGGG 
AAATACTTTG TGTTTGACCC CAAGAAAACA TCCATTGAAG AGTTCTTCGC AGACCTGCAG 
AATTTCCGGA ACATGTTCTT GCACGCTCTG AAAGAGAACC AGAAGAGACG GGAAGCAGAA 
GAAAAGATGA AAAGAGCCAA ACTTGCCAAA GAGAAGGCAG AGAAGGAACG GTTAGAGAAG 
CAGCAGAAGA AAGAACAACT GATTGATATG AATGCAGAGG GTGACGAAAC TGGCGTAATG 
GACAGCCTGC TTGAAGCTTT GAATTCGGGA GCTGCATTCA GAAGGAAAAG GGGTCCTCGG 
CAAGCGAACA GAAAGGCCGG CTGTGCCGTT ACCTCACTGC TAGCATCTGA GCTCACTAAA 
GAAGATGCAA TGACCTCAGT GCCCACTAAG AAGAAAAAAC CCGCTGAGAC GGATTTGATG 
GAAACAGATG TAGGACTGCA CGAAGATGCC CAGGATATGC TTACGCGAGC AAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031754640.1
CDS	208..4044
Translation	MEPPAGGGGYGGGPPPPSKGNKEKKKPKGQDEGGDGKSKKFTFKRLMVDELERFTSIRMKKEKEKPYPSHRNSTNFPDNSNPLQTLQELEDDVVLQLFEKMLDDMNLNPEKQQPLREKDIMIKREMVSQYLHTSKAGMSKKESSRSALMYIQDLKSGLGDNSLLLCLESLRVSLNNNPVSWVQNFGAEGLSCLLEILKRIQDEQASLRDVRSEHEIIRCLKAFMNNNFGIKAMLGTEDGILLLARAVDRSVPAMMIDSLKLLSALCILPQPEDMHERVLEALTERAEMEEMERFKPLLDGLNIASPVALKVSCMQLINAVISQGEELDYRVHIRSELMRSGLAKLLKELRNTENEDLKVQLGIFDEHSELDSDDLRGRLDDIRIEMDDFSDVFQVLLNTVKDSSAEPLFLSILQHLLLIRNDYDARPQYYKLIDEVISQIILQKNGADPDFKCKHVNIEIENLIDHFIDKKKVETSESKARDLVKKLDLELTARHELQVEMKAKESDFELKITELQGEKESLDAEKQEITADKQKMETELSQLVEQVEKLSKELEATKKEMASISTSAVLIPVPSASGNVPPPPPPPPPLPGATGIPPPPPLPGGSGIPPPPPLPGGSGIAPPLPAGFGIPPPPPLPGGFGIPPPPPLPGGSGIPPPPPLPGGSGIPPPPPLPGGSGIPPPPPLPGGSGIPPPPPFPGGPGIPPPPPFPGGFGIPPPPPPFPGGPGIPPPPPFPGGPGVPPPPPAFGGGVPPPPGLGGWGAPPPLPFGLAPKKNYKPEVQLKRPNWSKITPAELSEKSFWTQTKEDRFEKEELFAKITLTFSSQIKSSKAKKAEDGDDKKAAQKKKTKELKVLDSKTAQNLSIFLGSFRMPYEEIKNVILQVNEEVLTESMVSNLIKQLPEPDQLQMLTEFKNEYKDLAEPEQFGVVMCSVPRLRPRLNAILFKLLFNEQIENIKPDIVSVTAACEEVQKCQSFASLLEITLLVGNFMNAGSRNADAYGFDISFLCKLKDTKSADQKTTLLHFLVETCENDYPDVLKFPDEMVHVEKASRVSAETLQKNLDQMKKQIADLQRDIDSFPPTEDEKDKFVEKMTIFVKEAQEQYTKLCMMHENMLSLFQDLGKYFVFDPKKTSIEEFFADLQNFRNMFLHALKENQKRREAEEKMKRAKLAKEKAEKERLEKQQKKEQLIDMNAEGDETGVMDSLLEALNSGAAFRRKRGPRQANRKAGCAVTSLLASELTKEDAMTSVPTKKKKPAETDLMETDVGLHEDAQDMLTRAS

Target ORF information:

RefSeq Version	XM_031898780.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis diaphanous related formin 1 (diaph1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031898780.1

ORF Insert Sequence:

ATGGAACCGC CAGCCGGTGG AGGCGGATAT GGAGGGGGAC CCCCGCCACC TTCCAAGGGC 
AACAAGGAGA AGAAGAAGCC GAAGGGCCAA GATGAAGGTG GAGATGGCAA GTCCAAGAAA 
TTTACCTTTA AGCGGCTGAT GGTAGATGAG CTTGAACGAT TTACCAGTAT ACGGATGAAG 
AAAGAAAAGG AAAAGCCTTA TCCATCACAC AGAAACTCCA CCAACTTCCC AGATAACTCA 
AACCCCCTCC AAACCTTGCA GGAACTCGAA GATGATGTTG TGCTTCAGCT GTTTGAAAAA 
ATGCTGGATG ATATGAACCT CAATCCTGAG AAGCAGCAAC CGCTACGAGA AAAGGATATA 
ATGATAAAAA GAGAGATGGT TTCCCAATAT CTTCACACAT CCAAAGCTGG CATGAGCAAA 
AAGGAGAGTT CTCGTTCTGC ATTAATGTAC ATTCAAGACT TAAAATCCGG ACTAGGAGAC 
AATTCCCTCC TTTTGTGCTT AGAGTCACTA AGAGTATCTT TAAATAACAA TCCTGTCAGC 
TGGGTGCAGA ATTTTGGTGC AGAGGGTCTT TCGTGCCTTT TAGAAATTCT CAAAAGAATA 
CAGGATGAGC AAGCATCCTT GCGGGATGTT AGAAGCGAGC ATGAGATCAT CAGATGTCTG 
AAAGCTTTTA TGAATAACAA TTTTGGAATT AAGGCAATGC TGGGCACAGA AGATGGCATT 
TTGCTGTTAG CGAGAGCAGT AGATCGATCT GTCCCTGCAA TGATGATTGA TTCCTTGAAG 
CTGCTTTCTG CCCTGTGCAT CCTGCCACAA CCAGAGGACA TGCATGAGCG TGTTCTAGAA 
GCCCTTACGG AGAGAGCAGA GATGGAGGAA ATGGAACGAT TTAAACCTCT TCTGGATGGG 
TTAAACATTG CAAGTCCTGT GGCCTTGAAG GTGAGCTGTA TGCAGCTAAT CAATGCAGTG 
ATCAGCCAAG GAGAAGAGCT TGATTACAGA GTTCACATCC GAAGTGAATT GATGCGCTCG 
GGACTGGCCA AACTCCTAAA GGAACTACGT AATACAGAAA ATGAAGACCT GAAGGTACAA 
CTTGGTATTT TTGATGAGCA TTCTGAACTT GATTCTGATG ACTTGCGAGG ACGTTTGGAC 
GACATCCGCA TTGAAATGGA TGACTTCAGT GACGTCTTCC AAGTTCTTCT GAACACTGTC 
AAGGACTCCT CTGCAGAACC ATTATTTCTT TCCATCCTGC AGCATCTTCT TCTAATTAGA 
AATGATTATG ATGCCAGGCC GCAGTACTAT AAACTGATAG ATGAAGTTAT ATCACAGATA 
ATCCTTCAAA AAAATGGGGC TGATCCAGAT TTCAAATGCA AGCATGTAAA CATAGAAATC 
GAAAATCTGA TAGATCATTT TATCGACAAA AAGAAAGTAG AAACAAGCGA GTCAAAAGCA 
CGGGATCTGG TGAAAAAGTT GGACCTAGAG CTTACCGCAC GCCACGAACT CCAGGTGGAA 
ATGAAAGCCA AAGAGAGCGA CTTTGAGCTG AAAATAACAG AGCTGCAGGG GGAGAAGGAA 
TCTTTAGATG CAGAGAAGCA AGAAATCACT GCTGACAAGC AAAAAATGGA GACAGAACTC 
TCCCAACTTG TAGAGCAGGT TGAAAAATTG TCAAAGGAAC TTGAAGCTAC CAAAAAAGAA 
ATGGCATCAA TTTCCACATC TGCTGTTTTA ATCCCTGTAC CTTCTGCCTC TGGAAATGTG 
CCACCTCCAC CACCCCCTCC TCCTCCACTT CCAGGTGCAA CTGGTATTCC TCCACCTCCC 
CCACTTCCAG GTGGCTCTGG TATTCCTCCA CCACCCCCAC TTCCAGGTGG CTCTGGTATT 
GCTCCACCAC TTCCGGCTGG CTTTGGTATT CCTCCACCTC CCCCACTTCC GGGTGGCTTT 
GGTATTCCTC CACCTCCACC ACTTCCCGGT GGCTCTGGTA TTCCTCCACC TCCCCCACTT 
CCGGGTGGCT CTGGTATTCC TCCACCTCCC CCACTTCCGG GTGGCTCTGG TATTCCTCCA 
CCTCCCCCAC TTCCGGGTGG CTCTGGTATT CCTCCCCCAC CACCATTTCC GGGTGGCCCT 
GGAATTCCTC CCCCACCGCC ATTTCCAGGA GGCTTTGGAA TTCCTCCTCC TCCTCCTCCA 
TTTCCAGGTG GCCCTGGAAT TCCTCCTCCT CCTCCATTCC CTGGTGGCCC TGGGGTTCCA 
CCTCCACCTC CAGCATTTGG AGGTGGTGTT CCTCCACCAC CAGGACTTGG AGGCTGGGGG 
GCACCACCAC CCTTACCATT TGGCTTGGCT CCTAAGAAGA ATTATAAGCC AGAAGTGCAA 
CTGAAGCGAC CCAACTGGTC AAAGATCACT CCTGCTGAGC TTTCAGAGAA ATCGTTTTGG 
ACTCAGACAA AAGAAGATCG TTTTGAAAAA GAAGAGCTGT TTGCAAAAAT AACCCTCACT 
TTTTCTTCAC AGATCAAGTC TTCTAAAGCA AAAAAAGCAG AAGATGGTGA TGACAAAAAA 
GCAGCCCAGA AGAAAAAGAC AAAGGAGCTC AAAGTCTTGG ATTCCAAGAC AGCCCAAAAC 
CTATCAATCT TCCTTGGGTC GTTCCGCATG CCATACGAAG AGATCAAAAA TGTGATCCTA 
CAGGTCAATG AGGAAGTGCT GACTGAATCT ATGGTTTCAA ATCTGATCAA GCAGTTACCA 
GAACCAGATC AGTTACAGAT GTTAACAGAG TTCAAGAATG AGTACAAGGA TTTGGCAGAA 
CCAGAGCAGT TTGGAGTAGT GATGTGCAGT GTCCCCCGTT TGCGGCCACG ACTAAATGCC 
ATTCTCTTTA AACTTCTTTT TAATGAGCAA ATTGAAAATA TCAAGCCAGA CATTGTGTCA 
GTTACAGCTG CTTGCGAAGA AGTGCAAAAG TGCCAAAGCT TTGCTAGTCT CCTGGAAATC 
ACACTGCTGG TTGGGAACTT CATGAACGCA GGATCAAGGA ACGCTGATGC TTATGGCTTT 
GACATTAGCT TTCTCTGCAA GTTGAAAGAT ACCAAATCAG CGGACCAGAA GACCACACTG 
CTGCATTTCC TTGTTGAGAC TTGTGAGAAT GACTACCCTG ATGTACTGAA GTTTCCAGAT 
GAGATGGTTC ATGTAGAAAA GGCCAGTAGA GTGTCAGCTG AAACATTGCA AAAGAACCTG 
GATCAAATGA AAAAGCAGAT TGCAGACCTT CAAAGGGACA TTGATAGCTT CCCACCAACA 
GAAGATGAGA AGGATAAGTT TGTAGAAAAA ATGACCATAT TTGTCAAAGA AGCTCAAGAA 
CAGTATACTA AGCTTTGCAT GATGCACGAA AACATGCTGT CATTGTTCCA AGATTTGGGG 
AAATACTTTG TGTTTGACCC CAAGAAAACA TCCATTGAAG AGTTCTTCGC AGACCTGCAG 
AATTTCCGGA ACATGTTCTT GCACGCTCTG AAAGAGAACC AGAAGAGACG GGAAGCAGAA 
GAAAAGATGA AAAGAGCCAA ACTTGCCAAA GAGAAGGCAG AGAAGGAACG GTTAGAGAAG 
CAGCAGAAGA AAGAACAACT GATTGATATG AATGCAGAGG GTGACGAAAC TGGCGTAATG 
GACAGCCTGC TTGAAGCTTT GAATTCGGGA GCTGCATTCA GAAGGAAAAG GGGTCCTCGG 
CAAGCGAACA GAAAGGCCGG CTGTGCCGTT ACCTCACTGC TAGCATCTGA GCTCACTAAA 
GAAGATGCAA TGACCTCAGT GCCCACTAAG AAGAAAAAAC CCGCTGAGAC GGATTTGATG 
GAAACAGATG TAGGACTGCA CGAAGATGCC CAGGATATGC TTACGCGAGC AAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

diaph1 ( XM_012959694.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012815148.2 Xenopus tropicalis diaphanous related formin 1 (diaph1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa54438
Clone ID Related Accession (Same CDS sequence)	XM_012959694.3
Accession Version	XM_012959694.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein diaphanous homolog 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959694.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACCGC CAGCCGGTGG AGGCGGATAT GGAGGGGGAC CCCCGCCACC TTCCAAGGGC 
AACAAGGAGA AGAAGAAGCC GAAGGGCCAA GATGAAGGTG GAGATGGCAA GTCCAAGAAA 
TTTCTTGAAC GATTTACCAG TATACGGATG AAGAAAGAAA AGGAAAAGCC TTATCCATCA 
CACAGAAACT CCACCAACTT CCCAGATAAC TCAAACCCCC TCCAAACCTT GCAGGAACTC 
GAAGATGATG TTGTGCTTCA GCTGTTTGAA AAAATGCTGG ATGATATGAA CCTCAATCCT 
GAGAAGCAGC AACCGCTACG AGAAAAGGAT ATAATGATAA AAAGAGAGAT GGTTTCCCAA 
TATCTTCACA CATCCAAAGC TGGCATGAGC AAAAAGGAGA GTTCTCGTTC TGCATTAATG 
TACATTCAAG ACTTAAAATC CGGACTAGGA GACAATTCCC TCCTTTTGTG CTTAGAGTCA 
CTAAGAGTAT CTTTAAATAA CAATCCTGTC AGCTGGGTGC AGAATTTTGG TGCAGAGGGT 
CTTTCGTGCC TTTTAGAAAT TCTCAAAAGA ATACAGGATG AGCAAGCATC CTTGCGGGAT 
GTTAGAAGCG AGCATGAGAT CATCAGATGT CTGAAAGCTT TTATGAATAA CAATTTTGGA 
ATTAAGGCAA TGCTGGGCAC AGAAGATGGC ATTTTGCTGT TAGCGAGAGC AGTAGATCGA 
TCTGTCCCTG CAATGATGAT TGATTCCTTG AAGCTGCTTT CTGCCCTGTG CATCCTGCCA 
CAACCAGAGG ACATGCATGA GCGTGTTCTA GAAGCCCTTA CGGAGAGAGC AGAGATGGAG 
GAAATGGAAC GATTTAAACC TCTTCTGGAT GGGTTAAACA TTGCAAGTCC TGTGGCCTTG 
AAGGTGAGCT GTATGCAGCT AATCAATGCA GTGATCAGCC AAGGAGAAGA GCTTGATTAC 
AGAGTTCACA TCCGAAGTGA ATTGATGCGC TCGGGACTGG CCAAACTCCT AAAGGAACTA 
CGTAATACAG AAAATGAAGA CCTGAAGGTA CAACTTGGTA TTTTTGATGA GCATTCTGAA 
CTTGATTCTG ATGACTTGCG AGGACGTTTG GACGACATCC GCATTGAAAT GGATGACTTC 
AGTGACGTCT TCCAAGTTCT TCTGAACACT GTCAAGGACT CCTCTGCAGA ACCATTATTT 
CTTTCCATCC TGCAGCATCT TCTTCTAATT AGAAATGATT ATGATGCCAG GCCGCAGTAC 
TATAAACTGA TAGATGAAGT TATATCACAG ATAATCCTTC AAAAAAATGG GGCTGATCCA 
GATTTCAAAT GCAAGCATGT AAACATAGAA ATCGAAAATC TGATAGATCA TTTTATCGAC 
AAAAAGAAAG TAGAAACAAG CGAGTCAAAA GCACGGGATC TGGTGAAAAA GTTGGACCTA 
GAGCTTACCG CACGCCACGA ACTCCAGGTG GAAATGAAAG CCAAAGAGAG CGACTTTGAG 
CTGAAAATAA CAGAGCTGCA GGGGGAGAAG GAATCTTTAG ATGCAGAGAA GCAAGAAATC 
ACTGCTGACA AGCAAAAAAT GGAGACAGAA CTCTCCCAAC TTGTAGAGCA GGTTGAAAAA 
TTGTCAAAGG AACTTGAAGC TACCAAAAAA GAAATGGCAT CAATTTCCAC ATCTGCTGTT 
TTAATCCCTG TACCTTCTGC CTCTGGAAAT GTGCCACCTC CACCACCCCC TCCTCCTCCA 
CTTCCAGGTG CAACTGGTAT TCCTCCACCT CCCCCACTTC CAGGTGGCTC TGGTATTCCT 
CCACCACCCC CACTTCCAGG TGGCTCTGGT ATTGCTCCAC CACTTCCGGC TGGCTTTGGT 
ATTCCTCCAC CTCCCCCACT TCCGGGTGGC TTTGGTATTC CTCCACCTCC ACCACTTCCC 
GGTGGCTCTG GTATTCCTCC ACCTCCCCCA CTTCCGGGTG GCTCTGGTAT TCCTCCACCT 
CCCCCACTTC CGGGTGGCTC TGGTATTCCT CCACCTCCCC CACTTCCGGG TGGCTCTGGT 
ATTCCTCCCC CACCACCATT TCCGGGTGGC CCTGGAATTC CTCCCCCACC GCCATTTCCA 
GGAGGCTTTG GAATTCCTCC TCCTCCTCCT CCATTTCCAG GTGGCCCTGG AATTCCTCCT 
CCTCCTCCAT TCCCTGGTGG CCCTGGGGTT CCACCTCCAC CTCCAGCATT TGGAGGTGGT 
GTTCCTCCAC CACCAGGACT TGGAGGCTGG GGGGCACCAC CACCCTTACC ATTTGGCTTG 
GCTCCTAAGA AGAATTATAA GCCAGAAGTG CAACTGAAGC GACCCAACTG GTCAAAGATC 
ACTCCTGCTG AGCTTTCAGA GAAATCGTTT TGGACTCAGA CAAAAGAAGA TCGTTTTGAA 
AAAGAAGAGC TGTTTGCAAA AATAACCCTC ACTTTTTCTT CACAGATCAA GTCTTCTAAA 
GCAAAAAAAG CAGAAGATGG TGATGACAAA AAAGCAGCCC AGAAGAAAAA GACAAAGGAG 
CTCAAAGTCT TGGATTCCAA GACAGCCCAA AACCTATCAA TCTTCCTTGG GTCGTTCCGC 
ATGCCATACG AAGAGATCAA AAATGTGATC CTACAGGTCA ATGAGGAAGT GCTGACTGAA 
TCTATGGTTT CAAATCTGAT CAAGCAGTTA CCAGAACCAG ATCAGTTACA GATGTTAACA 
GAGTTCAAGA ATGAGTACAA GGATTTGGCA GAACCAGAGC AGTTTGGAGT AGTGATGTGC 
AGTGTCCCCC GTTTGCGGCC ACGACTAAAT GCCATTCTCT TTAAACTTCT TTTTAATGAG 
CAAATTGAAA ATATCAAGCC AGACATTGTG TCAGTTACAG CTGCTTGCGA AGAAGTGCAA 
AAGTGCCAAA GCTTTGCTAG TCTCCTGGAA ATCACACTGC TGGTTGGGAA CTTCATGAAC 
GCAGGATCAA GGAACGCTGA TGCTTATGGC TTTGACATTA GCTTTCTCTG CAAGTTGAAA 
GATACCAAAT CAGCGGACCA GAAGACCACA CTGCTGCATT TCCTTGTTGA GACTTGTGAG 
AATGACTACC CTGATGTACT GAAGTTTCCA GATGAGATGG TTCATGTAGA AAAGGCCAGT 
AGAGTGTCAG CTGAAACATT GCAAAAGAAC CTGGATCAAA TGAAAAAGCA GATTGCAGAC 
CTTCAAAGGG ACATTGATAG CTTCCCACCA ACAGAAGATG AGAAGGATAA GTTTGTAGAA 
AAAATGACAA TATTTGTCAA AGAAGCTCAA GAACAGTATA CTAAGCTTTG CATGATGCAC 
GAAAACATGC TGTCATTGTT CCAAGATTTG GGGAAATACT TTGTGTTTGA CCCCAAGAAA 
ACATCCATTG AAGAGTTCTT CGCAGACCTG CAGAATTTCC GGAACATGTT CTTGCACGCT 
CTGAAAGAGA ACCAGAAGAG ACGGGAAGCA GAAGAAAAGA TGAAAAGAGC CAAACTTGCC 
AAAGAGAAGG CAGAGAAGGA ACGGTTAGAG AAGCAGCAGA AGAAAGAACA ACTGATTGAT 
ATGAATGCAG AGGGTGACGA AACTGGCGTA ATGGACAGCC TGCTTGAAGC TTTGAATTCG 
GGAGCTGCAT TCAGAAGGAA AAGGGGTCCT CGGCAAGCGA ACAGAAAGGC CGGCTGTGCC 
GTTACCTCAC TGCTAGCATC TGAGCTCACT AAAGAAGATG CAATGACCTC AGTGCCCACT 
AAGAAGAAAA AACCCGCTGA GACGGATTTG ATGGAAACAG ATGTAGGACT GCACGAAGAT 
GCCCAGGATA TGCTTACGCG AGCAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012815148.2
CDS	316..4125
Translation	MEPPAGGGGYGGGPPPPSKGNKEKKKPKGQDEGGDGKSKKFLERFTSIRMKKEKEKPYPSHRNSTNFPDNSNPLQTLQELEDDVVLQLFEKMLDDMNLNPEKQQPLREKDIMIKREMVSQYLHTSKAGMSKKESSRSALMYIQDLKSGLGDNSLLLCLESLRVSLNNNPVSWVQNFGAEGLSCLLEILKRIQDEQASLRDVRSEHEIIRCLKAFMNNNFGIKAMLGTEDGILLLARAVDRSVPAMMIDSLKLLSALCILPQPEDMHERVLEALTERAEMEEMERFKPLLDGLNIASPVALKVSCMQLINAVISQGEELDYRVHIRSELMRSGLAKLLKELRNTENEDLKVQLGIFDEHSELDSDDLRGRLDDIRIEMDDFSDVFQVLLNTVKDSSAEPLFLSILQHLLLIRNDYDARPQYYKLIDEVISQIILQKNGADPDFKCKHVNIEIENLIDHFIDKKKVETSESKARDLVKKLDLELTARHELQVEMKAKESDFELKITELQGEKESLDAEKQEITADKQKMETELSQLVEQVEKLSKELEATKKEMASISTSAVLIPVPSASGNVPPPPPPPPPLPGATGIPPPPPLPGGSGIPPPPPLPGGSGIAPPLPAGFGIPPPPPLPGGFGIPPPPPLPGGSGIPPPPPLPGGSGIPPPPPLPGGSGIPPPPPLPGGSGIPPPPPFPGGPGIPPPPPFPGGFGIPPPPPPFPGGPGIPPPPPFPGGPGVPPPPPAFGGGVPPPPGLGGWGAPPPLPFGLAPKKNYKPEVQLKRPNWSKITPAELSEKSFWTQTKEDRFEKEELFAKITLTFSSQIKSSKAKKAEDGDDKKAAQKKKTKELKVLDSKTAQNLSIFLGSFRMPYEEIKNVILQVNEEVLTESMVSNLIKQLPEPDQLQMLTEFKNEYKDLAEPEQFGVVMCSVPRLRPRLNAILFKLLFNEQIENIKPDIVSVTAACEEVQKCQSFASLLEITLLVGNFMNAGSRNADAYGFDISFLCKLKDTKSADQKTTLLHFLVETCENDYPDVLKFPDEMVHVEKASRVSAETLQKNLDQMKKQIADLQRDIDSFPPTEDEKDKFVEKMTIFVKEAQEQYTKLCMMHENMLSLFQDLGKYFVFDPKKTSIEEFFADLQNFRNMFLHALKENQKRREAEEKMKRAKLAKEKAEKERLEKQQKKEQLIDMNAEGDETGVMDSLLEALNSGAAFRRKRGPRQANRKAGCAVTSLLASELTKEDAMTSVPTKKKKPAETDLMETDVGLHEDAQDMLTRAS

Target ORF information:

RefSeq Version	XM_012959694.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis diaphanous related formin 1 (diaph1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012959694.3

ORF Insert Sequence:

ATGGAACCGC CAGCCGGTGG AGGCGGATAT GGAGGGGGAC CCCCGCCACC TTCCAAGGGC 
AACAAGGAGA AGAAGAAGCC GAAGGGCCAA GATGAAGGTG GAGATGGCAA GTCCAAGAAA 
TTTCTTGAAC GATTTACCAG TATACGGATG AAGAAAGAAA AGGAAAAGCC TTATCCATCA 
CACAGAAACT CCACCAACTT CCCAGATAAC TCAAACCCCC TCCAAACCTT GCAGGAACTC 
GAAGATGATG TTGTGCTTCA GCTGTTTGAA AAAATGCTGG ATGATATGAA CCTCAATCCT 
GAGAAGCAGC AACCGCTACG AGAAAAGGAT ATAATGATAA AAAGAGAGAT GGTTTCCCAA 
TATCTTCACA CATCCAAAGC TGGCATGAGC AAAAAGGAGA GTTCTCGTTC TGCATTAATG 
TACATTCAAG ACTTAAAATC CGGACTAGGA GACAATTCCC TCCTTTTGTG CTTAGAGTCA 
CTAAGAGTAT CTTTAAATAA CAATCCTGTC AGCTGGGTGC AGAATTTTGG TGCAGAGGGT 
CTTTCGTGCC TTTTAGAAAT TCTCAAAAGA ATACAGGATG AGCAAGCATC CTTGCGGGAT 
GTTAGAAGCG AGCATGAGAT CATCAGATGT CTGAAAGCTT TTATGAATAA CAATTTTGGA 
ATTAAGGCAA TGCTGGGCAC AGAAGATGGC ATTTTGCTGT TAGCGAGAGC AGTAGATCGA 
TCTGTCCCTG CAATGATGAT TGATTCCTTG AAGCTGCTTT CTGCCCTGTG CATCCTGCCA 
CAACCAGAGG ACATGCATGA GCGTGTTCTA GAAGCCCTTA CGGAGAGAGC AGAGATGGAG 
GAAATGGAAC GATTTAAACC TCTTCTGGAT GGGTTAAACA TTGCAAGTCC TGTGGCCTTG 
AAGGTGAGCT GTATGCAGCT AATCAATGCA GTGATCAGCC AAGGAGAAGA GCTTGATTAC 
AGAGTTCACA TCCGAAGTGA ATTGATGCGC TCGGGACTGG CCAAACTCCT AAAGGAACTA 
CGTAATACAG AAAATGAAGA CCTGAAGGTA CAACTTGGTA TTTTTGATGA GCATTCTGAA 
CTTGATTCTG ATGACTTGCG AGGACGTTTG GACGACATCC GCATTGAAAT GGATGACTTC 
AGTGACGTCT TCCAAGTTCT TCTGAACACT GTCAAGGACT CCTCTGCAGA ACCATTATTT 
CTTTCCATCC TGCAGCATCT TCTTCTAATT AGAAATGATT ATGATGCCAG GCCGCAGTAC 
TATAAACTGA TAGATGAAGT TATATCACAG ATAATCCTTC AAAAAAATGG GGCTGATCCA 
GATTTCAAAT GCAAGCATGT AAACATAGAA ATCGAAAATC TGATAGATCA TTTTATCGAC 
AAAAAGAAAG TAGAAACAAG CGAGTCAAAA GCACGGGATC TGGTGAAAAA GTTGGACCTA 
GAGCTTACCG CACGCCACGA ACTCCAGGTG GAAATGAAAG CCAAAGAGAG CGACTTTGAG 
CTGAAAATAA CAGAGCTGCA GGGGGAGAAG GAATCTTTAG ATGCAGAGAA GCAAGAAATC 
ACTGCTGACA AGCAAAAAAT GGAGACAGAA CTCTCCCAAC TTGTAGAGCA GGTTGAAAAA 
TTGTCAAAGG AACTTGAAGC TACCAAAAAA GAAATGGCAT CAATTTCCAC ATCTGCTGTT 
TTAATCCCTG TACCTTCTGC CTCTGGAAAT GTGCCACCTC CACCACCCCC TCCTCCTCCA 
CTTCCAGGTG CAACTGGTAT TCCTCCACCT CCCCCACTTC CAGGTGGCTC TGGTATTCCT 
CCACCACCCC CACTTCCAGG TGGCTCTGGT ATTGCTCCAC CACTTCCGGC TGGCTTTGGT 
ATTCCTCCAC CTCCCCCACT TCCGGGTGGC TTTGGTATTC CTCCACCTCC ACCACTTCCC 
GGTGGCTCTG GTATTCCTCC ACCTCCCCCA CTTCCGGGTG GCTCTGGTAT TCCTCCACCT 
CCCCCACTTC CGGGTGGCTC TGGTATTCCT CCACCTCCCC CACTTCCGGG TGGCTCTGGT 
ATTCCTCCCC CACCACCATT TCCGGGTGGC CCTGGAATTC CTCCCCCACC GCCATTTCCA 
GGAGGCTTTG GAATTCCTCC TCCTCCTCCT CCATTTCCAG GTGGCCCTGG AATTCCTCCT 
CCTCCTCCAT TCCCTGGTGG CCCTGGGGTT CCACCTCCAC CTCCAGCATT TGGAGGTGGT 
GTTCCTCCAC CACCAGGACT TGGAGGCTGG GGGGCACCAC CACCCTTACC ATTTGGCTTG 
GCTCCTAAGA AGAATTATAA GCCAGAAGTG CAACTGAAGC GACCCAACTG GTCAAAGATC 
ACTCCTGCTG AGCTTTCAGA GAAATCGTTT TGGACTCAGA CAAAAGAAGA TCGTTTTGAA 
AAAGAAGAGC TGTTTGCAAA AATAACCCTC ACTTTTTCTT CACAGATCAA GTCTTCTAAA 
GCAAAAAAAG CAGAAGATGG TGATGACAAA AAAGCAGCCC AGAAGAAAAA GACAAAGGAG 
CTCAAAGTCT TGGATTCCAA GACAGCCCAA AACCTATCAA TCTTCCTTGG GTCGTTCCGC 
ATGCCATACG AAGAGATCAA AAATGTGATC CTACAGGTCA ATGAGGAAGT GCTGACTGAA 
TCTATGGTTT CAAATCTGAT CAAGCAGTTA CCAGAACCAG ATCAGTTACA GATGTTAACA 
GAGTTCAAGA ATGAGTACAA GGATTTGGCA GAACCAGAGC AGTTTGGAGT AGTGATGTGC 
AGTGTCCCCC GTTTGCGGCC ACGACTAAAT GCCATTCTCT TTAAACTTCT TTTTAATGAG 
CAAATTGAAA ATATCAAGCC AGACATTGTG TCAGTTACAG CTGCTTGCGA AGAAGTGCAA 
AAGTGCCAAA GCTTTGCTAG TCTCCTGGAA ATCACACTGC TGGTTGGGAA CTTCATGAAC 
GCAGGATCAA GGAACGCTGA TGCTTATGGC TTTGACATTA GCTTTCTCTG CAAGTTGAAA 
GATACCAAAT CAGCGGACCA GAAGACCACA CTGCTGCATT TCCTTGTTGA GACTTGTGAG 
AATGACTACC CTGATGTACT GAAGTTTCCA GATGAGATGG TTCATGTAGA AAAGGCCAGT 
AGAGTGTCAG CTGAAACATT GCAAAAGAAC CTGGATCAAA TGAAAAAGCA GATTGCAGAC 
CTTCAAAGGG ACATTGATAG CTTCCCACCA ACAGAAGATG AGAAGGATAA GTTTGTAGAA 
AAAATGACAA TATTTGTCAA AGAAGCTCAA GAACAGTATA CTAAGCTTTG CATGATGCAC 
GAAAACATGC TGTCATTGTT CCAAGATTTG GGGAAATACT TTGTGTTTGA CCCCAAGAAA 
ACATCCATTG AAGAGTTCTT CGCAGACCTG CAGAATTTCC GGAACATGTT CTTGCACGCT 
CTGAAAGAGA ACCAGAAGAG ACGGGAAGCA GAAGAAAAGA TGAAAAGAGC CAAACTTGCC 
AAAGAGAAGG CAGAGAAGGA ACGGTTAGAG AAGCAGCAGA AGAAAGAACA ACTGATTGAT 
ATGAATGCAG AGGGTGACGA AACTGGCGTA ATGGACAGCC TGCTTGAAGC TTTGAATTCG 
GGAGCTGCAT TCAGAAGGAA AAGGGGTCCT CGGCAAGCGA ACAGAAAGGC CGGCTGTGCC 
GTTACCTCAC TGCTAGCATC TGAGCTCACT AAAGAAGATG CAATGACCTC AGTGCCCACT 
AAGAAGAAAA AACCCGCTGA GACGGATTTG ATGGAAACAG ATGTAGGACT GCACGAAGAT 
GCCCAGGATA TGCTTACGCG AGCAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

diaph1 ( XM_031898781.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031754641.1 Xenopus tropicalis diaphanous related formin 1 (diaph1), transcript variant X3, mRNA.

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CloneID	OXa54439
Clone ID Related Accession (Same CDS sequence)	XM_031898781.1
Accession Version	XM_031898781.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3807bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein diaphanous homolog 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACCGC CAGCCGGTGG AGGCGGATAT GGAGGGGGAC CCCCGCCACC TTCCAAGGGC 
AACAAGGAGA AGAAGAAGCC GAAGGGCCAA GATGAAGGTG GAGATGGCAA GTCCAAGAAA 
TTTACCTTTA AGCGGCTGAT GGTAGATGAG CTTGAACGAT TTACCAGTAT ACGGATGAAG 
AAAGAAAAGG AAAAGCCTTA TCCATCACAC AGAAACTCCA CCAACTTCCC AGATAACTCA 
AACCCCCTCC AAACCTTGCA GGAACTCGAA GATGATGTTG TGCTTCAGCT GTTTGAAAAA 
ATGCTGGATG ATATGAACCT CAATCCTGAG AAGCAGCAAC CGCTACGAGA AAAGGATATA 
ATGATAAAAA GAGAGATGGT TTCCCAATAT CTTCACACAT CCAAAGCTGG CATGAGCAAA 
AAGGAGAGTT CTCGTTCTGC ATTAATGTAC ATTCAAGACT TAAAATCCGG ACTAGGAGAC 
AATTCCCTCC TTTTGTGCTT AGAGTCACTA AGAGTATCTT TAAATAACAA TCCTGTCAGC 
TGGGTGCAGA ATTTTGGTGC AGAGGGTCTT TCGTGCCTTT TAGAAATTCT CAAAAGAATA 
CAGGATGAGC AAGCATCCTT GCGGGATGTT AGAAGCGAGC ATGAGATCAT CAGATGTCTG 
AAAGCTTTTA TGAATAACAA TTTTGGAATT AAGGCAATGC TGGGCACAGA AGATGGCATT 
TTGCTGTTAG CGAGAGCAGT AGATCGATCT GTCCCTGCAA TGATGATTGA TTCCTTGAAG 
CTGCTTTCTG CCCTGTGCAT CCTGCCACAA CCAGAGGACA TGCATGAGCG TGTTCTAGAA 
GCCCTTACGG AGAGAGCAGA GATGGAGGAA ATGGAACGAT TTAAACCTCT TCTGGATGGG 
TTAAACATTG CAAGTCCTGT GGCCTTGAAG GTGAGCTGTA TGCAGCTAAT CAATGCAGTG 
ATCAGCCAAG GAGAAGAGCT TGATTACAGA GTTCACATCC GAAGTGAATT GATGCGCTCG 
GGACTGGCCA AACTCCTAAA GGAACTACGT AATACAGAAA ATGAAGACCT GAAGGTACAA 
CTTGGTATTT TTGATGAGCA TTCTGAACTT GATTCTGATG ACTTGCGAGG ACGTTTGGAC 
GACATCCGCA TTGAAATGGA TGACTTCAGT GACGTCTTCC AAGTTCTTCT GAACACTGTC 
AAGGACTCCT CTGCAGAACC ATTATTTCTT TCCATCCTGC AGCATCTTCT TCTAATTAGA 
AATGATTATG ATGCCAGGCC GCAGTACTAT AAACTGATAG ATGAAGTTAT ATCACAGATA 
ATCCTTCAAA AAAATGGGGC TGATCCAGAT TTCAAATGCA AGCATGTAAA CATAGAAATC 
GAAAATCTGA TAGATCATTT TATCGACAAA AAGAAAGTAG AAACAAGCGA GTCAAAAGCA 
CGGGATCTGG TGAAAAAGTT GGACCTAGAG CTTACCGCAC GCCACGAACT CCAGGTGGAA 
ATGAAAGCCA AAGAGAGCGA CTTTGAGCTG AAAATAACAG AGCTGCAGGG GGAGAAGGAA 
TCTTTAGATG CAGAGAAGCA AGAAATCACT GCTGACAAGC AAAAAATGGA GACAGAACTC 
TCCCAACTTG TAGAGCAGGT TGAAAAATTG TCAAAGGAAC TTGAAGCTAC CAAAAAAGAA 
ATGGCATCAA TTTCCACATC TGCTGTTTTA ATCCCTGTAC CTTCTGCCTC TGGAAATGTG 
CCACCTCCAC CACCCCCTCC TCCTCCACTT CCAGGTGCAA CTGGTATTCC TCCACCTCCC 
CCACTTCCAG GTGGCTCTGG TATTCCTCCA CCACCCCCAC TTCCAGGTGG CTCTGGTATT 
CCTCCACCTC CCCCACTTCC GGGTGGCTTT GGTATTCCTC CACCTCCACC ACTTCCCGGT 
GGCTCTGGTA TTCCTCCACC TCCCCCACTT CCGGGTGGCT CTGGTATTCC TCCACCTCCC 
CCACTTCCGG GTGGCTCTGG TATTCCTCCA CCTCCCCCAC TTCCGGGTGG CTCTGGTATT 
CCTCCCCCAC CACCATTTCC GGGTGGCCCT GGAATTCCTC CCCCACCGCC ATTTCCAGGA 
GGCTTTGGAA TTCCTCCTCC TCCTCCTCCA TTTCCAGGTG GCCCTGGAAT TCCTCCTCCT 
CCTCCATTCC CTGGTGGCCC TGGGGTTCCA CCTCCACCTC CAGCATTTGG AGGTGGTGTT 
CCTCCACCAC CAGGACTTGG AGGCTGGGGG GCACCACCAC CCTTACCATT TGGCTTGGCT 
CCTAAGAAGA ATTATAAGCC AGAAGTGCAA CTGAAGCGAC CCAACTGGTC AAAGATCACT 
CCTGCTGAGC TTTCAGAGAA ATCGTTTTGG ACTCAGACAA AAGAAGATCG TTTTGAAAAA 
GAAGAGCTGT TTGCAAAAAT AACCCTCACT TTTTCTTCAC AGATCAAGTC TTCTAAAGCA 
AAAAAAGCAG AAGATGGTGA TGACAAAAAA GCAGCCCAGA AGAAAAAGAC AAAGGAGCTC 
AAAGTCTTGG ATTCCAAGAC AGCCCAAAAC CTATCAATCT TCCTTGGGTC GTTCCGCATG 
CCATACGAAG AGATCAAAAA TGTGATCCTA CAGGTCAATG AGGAAGTGCT GACTGAATCT 
ATGGTTTCAA ATCTGATCAA GCAGTTACCA GAACCAGATC AGTTACAGAT GTTAACAGAG 
TTCAAGAATG AGTACAAGGA TTTGGCAGAA CCAGAGCAGT TTGGAGTAGT GATGTGCAGT 
GTCCCCCGTT TGCGGCCACG ACTAAATGCC ATTCTCTTTA AACTTCTTTT TAATGAGCAA 
ATTGAAAATA TCAAGCCAGA CATTGTGTCA GTTACAGCTG CTTGCGAAGA AGTGCAAAAG 
TGCCAAAGCT TTGCTAGTCT CCTGGAAATC ACACTGCTGG TTGGGAACTT CATGAACGCA 
GGATCAAGGA ACGCTGATGC TTATGGCTTT GACATTAGCT TTCTCTGCAA GTTGAAAGAT 
ACCAAATCAG CGGACCAGAA GACCACACTG CTGCATTTCC TTGTTGAGAC TTGTGAGAAT 
GACTACCCTG ATGTACTGAA GTTTCCAGAT GAGATGGTTC ATGTAGAAAA GGCCAGTAGA 
GTGTCAGCTG AAACATTGCA AAAGAACCTG GATCAAATGA AAAAGCAGAT TGCAGACCTT 
CAAAGGGACA TTGATAGCTT CCCACCAACA GAAGATGAGA AGGATAAGTT TGTAGAAAAA 
ATGACAATAT TTGTCAAAGA AGCTCAAGAA CAGTATACTA AGCTTTGCAT GATGCACGAA 
AACATGCTGT CATTGTTCCA AGATTTGGGG AAATACTTTG TGTTTGACCC CAAGAAAACA 
TCCATTGAAG AGTTCTTCGC AGACCTGCAG AATTTCCGGA ACATGTTCTT GCACGCTCTG 
AAAGAGAACC AGAAGAGACG GGAAGCAGAA GAAAAGATGA AAAGAGCCAA ACTTGCCAAA 
GAGAAGGCAG AGAAGGAACG GTTAGAGAAG CAGCAGAAGA AAGAACAACT GATTGATATG 
AATGCAGAGG GTGACGAAAC TGGCGTAATG GACAGCCTGC TTGAAGCTTT GAATTCGGGA 
GCTGCATTCA GAAGGAAAAG GGGTCCTCGG CAAGCGAACA GAAAGGCCGG CTGTGCCGTT 
ACCTCACTGC TAGCATCTGA GCTCACTAAA GAAGATGCAA TGACCTCAGT GCCCACTAAG 
AAGAAAAAAC CCGCTGAGAC GGATTTGATG GAAACAGATG TAGGACTGCA CGAAGATGCC 
CAGGATATGC TTACGCGAGC AAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031754641.1
CDS	208..4014
Translation	MEPPAGGGGYGGGPPPPSKGNKEKKKPKGQDEGGDGKSKKFTFKRLMVDELERFTSIRMKKEKEKPYPSHRNSTNFPDNSNPLQTLQELEDDVVLQLFEKMLDDMNLNPEKQQPLREKDIMIKREMVSQYLHTSKAGMSKKESSRSALMYIQDLKSGLGDNSLLLCLESLRVSLNNNPVSWVQNFGAEGLSCLLEILKRIQDEQASLRDVRSEHEIIRCLKAFMNNNFGIKAMLGTEDGILLLARAVDRSVPAMMIDSLKLLSALCILPQPEDMHERVLEALTERAEMEEMERFKPLLDGLNIASPVALKVSCMQLINAVISQGEELDYRVHIRSELMRSGLAKLLKELRNTENEDLKVQLGIFDEHSELDSDDLRGRLDDIRIEMDDFSDVFQVLLNTVKDSSAEPLFLSILQHLLLIRNDYDARPQYYKLIDEVISQIILQKNGADPDFKCKHVNIEIENLIDHFIDKKKVETSESKARDLVKKLDLELTARHELQVEMKAKESDFELKITELQGEKESLDAEKQEITADKQKMETELSQLVEQVEKLSKELEATKKEMASISTSAVLIPVPSASGNVPPPPPPPPPLPGATGIPPPPPLPGGSGIPPPPPLPGGSGIPPPPPLPGGFGIPPPPPLPGGSGIPPPPPLPGGSGIPPPPPLPGGSGIPPPPPLPGGSGIPPPPPFPGGPGIPPPPPFPGGFGIPPPPPPFPGGPGIPPPPPFPGGPGVPPPPPAFGGGVPPPPGLGGWGAPPPLPFGLAPKKNYKPEVQLKRPNWSKITPAELSEKSFWTQTKEDRFEKEELFAKITLTFSSQIKSSKAKKAEDGDDKKAAQKKKTKELKVLDSKTAQNLSIFLGSFRMPYEEIKNVILQVNEEVLTESMVSNLIKQLPEPDQLQMLTEFKNEYKDLAEPEQFGVVMCSVPRLRPRLNAILFKLLFNEQIENIKPDIVSVTAACEEVQKCQSFASLLEITLLVGNFMNAGSRNADAYGFDISFLCKLKDTKSADQKTTLLHFLVETCENDYPDVLKFPDEMVHVEKASRVSAETLQKNLDQMKKQIADLQRDIDSFPPTEDEKDKFVEKMTIFVKEAQEQYTKLCMMHENMLSLFQDLGKYFVFDPKKTSIEEFFADLQNFRNMFLHALKENQKRREAEEKMKRAKLAKEKAEKERLEKQQKKEQLIDMNAEGDETGVMDSLLEALNSGAAFRRKRGPRQANRKAGCAVTSLLASELTKEDAMTSVPTKKKKPAETDLMETDVGLHEDAQDMLTRAS

Target ORF information:

RefSeq Version	XM_031898781.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis diaphanous related formin 1 (diaph1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031898781.1

ORF Insert Sequence:

ATGGAACCGC CAGCCGGTGG AGGCGGATAT GGAGGGGGAC CCCCGCCACC TTCCAAGGGC 
AACAAGGAGA AGAAGAAGCC GAAGGGCCAA GATGAAGGTG GAGATGGCAA GTCCAAGAAA 
TTTACCTTTA AGCGGCTGAT GGTAGATGAG CTTGAACGAT TTACCAGTAT ACGGATGAAG 
AAAGAAAAGG AAAAGCCTTA TCCATCACAC AGAAACTCCA CCAACTTCCC AGATAACTCA 
AACCCCCTCC AAACCTTGCA GGAACTCGAA GATGATGTTG TGCTTCAGCT GTTTGAAAAA 
ATGCTGGATG ATATGAACCT CAATCCTGAG AAGCAGCAAC CGCTACGAGA AAAGGATATA 
ATGATAAAAA GAGAGATGGT TTCCCAATAT CTTCACACAT CCAAAGCTGG CATGAGCAAA 
AAGGAGAGTT CTCGTTCTGC ATTAATGTAC ATTCAAGACT TAAAATCCGG ACTAGGAGAC 
AATTCCCTCC TTTTGTGCTT AGAGTCACTA AGAGTATCTT TAAATAACAA TCCTGTCAGC 
TGGGTGCAGA ATTTTGGTGC AGAGGGTCTT TCGTGCCTTT TAGAAATTCT CAAAAGAATA 
CAGGATGAGC AAGCATCCTT GCGGGATGTT AGAAGCGAGC ATGAGATCAT CAGATGTCTG 
AAAGCTTTTA TGAATAACAA TTTTGGAATT AAGGCAATGC TGGGCACAGA AGATGGCATT 
TTGCTGTTAG CGAGAGCAGT AGATCGATCT GTCCCTGCAA TGATGATTGA TTCCTTGAAG 
CTGCTTTCTG CCCTGTGCAT CCTGCCACAA CCAGAGGACA TGCATGAGCG TGTTCTAGAA 
GCCCTTACGG AGAGAGCAGA GATGGAGGAA ATGGAACGAT TTAAACCTCT TCTGGATGGG 
TTAAACATTG CAAGTCCTGT GGCCTTGAAG GTGAGCTGTA TGCAGCTAAT CAATGCAGTG 
ATCAGCCAAG GAGAAGAGCT TGATTACAGA GTTCACATCC GAAGTGAATT GATGCGCTCG 
GGACTGGCCA AACTCCTAAA GGAACTACGT AATACAGAAA ATGAAGACCT GAAGGTACAA 
CTTGGTATTT TTGATGAGCA TTCTGAACTT GATTCTGATG ACTTGCGAGG ACGTTTGGAC 
GACATCCGCA TTGAAATGGA TGACTTCAGT GACGTCTTCC AAGTTCTTCT GAACACTGTC 
AAGGACTCCT CTGCAGAACC ATTATTTCTT TCCATCCTGC AGCATCTTCT TCTAATTAGA 
AATGATTATG ATGCCAGGCC GCAGTACTAT AAACTGATAG ATGAAGTTAT ATCACAGATA 
ATCCTTCAAA AAAATGGGGC TGATCCAGAT TTCAAATGCA AGCATGTAAA CATAGAAATC 
GAAAATCTGA TAGATCATTT TATCGACAAA AAGAAAGTAG AAACAAGCGA GTCAAAAGCA 
CGGGATCTGG TGAAAAAGTT GGACCTAGAG CTTACCGCAC GCCACGAACT CCAGGTGGAA 
ATGAAAGCCA AAGAGAGCGA CTTTGAGCTG AAAATAACAG AGCTGCAGGG GGAGAAGGAA 
TCTTTAGATG CAGAGAAGCA AGAAATCACT GCTGACAAGC AAAAAATGGA GACAGAACTC 
TCCCAACTTG TAGAGCAGGT TGAAAAATTG TCAAAGGAAC TTGAAGCTAC CAAAAAAGAA 
ATGGCATCAA TTTCCACATC TGCTGTTTTA ATCCCTGTAC CTTCTGCCTC TGGAAATGTG 
CCACCTCCAC CACCCCCTCC TCCTCCACTT CCAGGTGCAA CTGGTATTCC TCCACCTCCC 
CCACTTCCAG GTGGCTCTGG TATTCCTCCA CCACCCCCAC TTCCAGGTGG CTCTGGTATT 
CCTCCACCTC CCCCACTTCC GGGTGGCTTT GGTATTCCTC CACCTCCACC ACTTCCCGGT 
GGCTCTGGTA TTCCTCCACC TCCCCCACTT CCGGGTGGCT CTGGTATTCC TCCACCTCCC 
CCACTTCCGG GTGGCTCTGG TATTCCTCCA CCTCCCCCAC TTCCGGGTGG CTCTGGTATT 
CCTCCCCCAC CACCATTTCC GGGTGGCCCT GGAATTCCTC CCCCACCGCC ATTTCCAGGA 
GGCTTTGGAA TTCCTCCTCC TCCTCCTCCA TTTCCAGGTG GCCCTGGAAT TCCTCCTCCT 
CCTCCATTCC CTGGTGGCCC TGGGGTTCCA CCTCCACCTC CAGCATTTGG AGGTGGTGTT 
CCTCCACCAC CAGGACTTGG AGGCTGGGGG GCACCACCAC CCTTACCATT TGGCTTGGCT 
CCTAAGAAGA ATTATAAGCC AGAAGTGCAA CTGAAGCGAC CCAACTGGTC AAAGATCACT 
CCTGCTGAGC TTTCAGAGAA ATCGTTTTGG ACTCAGACAA AAGAAGATCG TTTTGAAAAA 
GAAGAGCTGT TTGCAAAAAT AACCCTCACT TTTTCTTCAC AGATCAAGTC TTCTAAAGCA 
AAAAAAGCAG AAGATGGTGA TGACAAAAAA GCAGCCCAGA AGAAAAAGAC AAAGGAGCTC 
AAAGTCTTGG ATTCCAAGAC AGCCCAAAAC CTATCAATCT TCCTTGGGTC GTTCCGCATG 
CCATACGAAG AGATCAAAAA TGTGATCCTA CAGGTCAATG AGGAAGTGCT GACTGAATCT 
ATGGTTTCAA ATCTGATCAA GCAGTTACCA GAACCAGATC AGTTACAGAT GTTAACAGAG 
TTCAAGAATG AGTACAAGGA TTTGGCAGAA CCAGAGCAGT TTGGAGTAGT GATGTGCAGT 
GTCCCCCGTT TGCGGCCACG ACTAAATGCC ATTCTCTTTA AACTTCTTTT TAATGAGCAA 
ATTGAAAATA TCAAGCCAGA CATTGTGTCA GTTACAGCTG CTTGCGAAGA AGTGCAAAAG 
TGCCAAAGCT TTGCTAGTCT CCTGGAAATC ACACTGCTGG TTGGGAACTT CATGAACGCA 
GGATCAAGGA ACGCTGATGC TTATGGCTTT GACATTAGCT TTCTCTGCAA GTTGAAAGAT 
ACCAAATCAG CGGACCAGAA GACCACACTG CTGCATTTCC TTGTTGAGAC TTGTGAGAAT 
GACTACCCTG ATGTACTGAA GTTTCCAGAT GAGATGGTTC ATGTAGAAAA GGCCAGTAGA 
GTGTCAGCTG AAACATTGCA AAAGAACCTG GATCAAATGA AAAAGCAGAT TGCAGACCTT 
CAAAGGGACA TTGATAGCTT CCCACCAACA GAAGATGAGA AGGATAAGTT TGTAGAAAAA 
ATGACAATAT TTGTCAAAGA AGCTCAAGAA CAGTATACTA AGCTTTGCAT GATGCACGAA 
AACATGCTGT CATTGTTCCA AGATTTGGGG AAATACTTTG TGTTTGACCC CAAGAAAACA 
TCCATTGAAG AGTTCTTCGC AGACCTGCAG AATTTCCGGA ACATGTTCTT GCACGCTCTG 
AAAGAGAACC AGAAGAGACG GGAAGCAGAA GAAAAGATGA AAAGAGCCAA ACTTGCCAAA 
GAGAAGGCAG AGAAGGAACG GTTAGAGAAG CAGCAGAAGA AAGAACAACT GATTGATATG 
AATGCAGAGG GTGACGAAAC TGGCGTAATG GACAGCCTGC TTGAAGCTTT GAATTCGGGA 
GCTGCATTCA GAAGGAAAAG GGGTCCTCGG CAAGCGAACA GAAAGGCCGG CTGTGCCGTT 
ACCTCACTGC TAGCATCTGA GCTCACTAAA GAAGATGCAA TGACCTCAGT GCCCACTAAG 
AAGAAAAAAC CCGCTGAGAC GGATTTGATG GAAACAGATG TAGGACTGCA CGAAGATGCC 
CAGGATATGC TTACGCGAGC AAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

diaph1 ( XM_012959695.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012815149.2 Xenopus tropicalis diaphanous related formin 1 (diaph1), transcript variant X4, mRNA.

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CloneID	OXa54440
Clone ID Related Accession (Same CDS sequence)	XM_012959695.3
Accession Version	XM_012959695.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein diaphanous homolog 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959695.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACCGC CAGCCGGTGG AGGCGGATAT GGAGGGGGAC CCCCGCCACC TTCCAAGGGC 
AACAAGGAGA AGAAGAAGCC GAAGGGCCAA GATGAAGGTG GAGATGGCAA GTCCAAGAAA 
TTTACCTTTA AGCGGCTGAT GGTAGATGAG CTTGAACGAT TTACCAGTAT ACGGATGAAG 
AAAGAAAAGG AAAAGCCTTA TCCATCACAC AGAAACTCCA CCAACTTCCC AGATAACTCA 
AACCCCCTCC AAACCTTGCA GGAACTCGAA GATGATGTTG TGCTTCAGCT GTTTGAAAAA 
ATGCTGGATG ATATGAACCT CAATCCTGAG AAGCAGCAAC CGCTACGAGA AAAGGATATA 
ATGATAAAAA GAGAGATGGT TTCCCAATAT CTTCACACAT CCAAAGCTGG CATGAGCAAA 
AAGGAGAGTT CTCGTTCTGC ATTAATGTAC ATTCAAGACT TAAAATCCGG ACTAGGAGAC 
AATTCCCTCC TTTTGTGCTT AGAGTCACTA AGAGTATCTT TAAATAACAA TCCTGTCAGC 
TGGGTGCAGA ATTTTGGTGC AGAGGGTCTT TCGTGCCTTT TAGAAATTCT CAAAAGAATA 
CAGGATGAGC AAGCATCCTT GCGGGATGTT AGAAGCGAGC ATGAGATCAT CAGATGTCTG 
AAAGCTTTTA TGAATAACAA TTTTGGAATT AAGGCAATGC TGGGCACAGA AGATGGCATT 
TTGCTGTTAG CGAGAGCAGT AGATCGATCT GTCCCTGCAA TGATGATTGA TTCCTTGAAG 
CTGCTTTCTG CCCTGTGCAT CCTGCCACAA CCAGAGGACA TGCATGAGCG TGTTCTAGAA 
GCCCTTACGG AGAGAGCAGA GATGGAGGAA ATGGAACGAT TTAAACCTCT TCTGGATGGG 
TTAAACATTG CAAGTCCTGT GGCCTTGAAG GTGAGCTGTA TGCAGCTAAT CAATGCAGTG 
ATCAGCCAAG GAGAAGAGCT TGATTACAGA GTTCACATCC GAAGTGAATT GATGCGCTCG 
GGACTGGCCA AACTCCTAAA GGAACTACGT AATACAGAAA ATGAAGACCT GAAGGTACAA 
CTTGGTATTT TTGATGAGCA TTCTGAACTT GATTCTGATG ACTTGCGAGG ACGTTTGGAC 
GACATCCGCA TTGAAATGGA TGACTTCAGT GACGTCTTCC AAGTTCTTCT GAACACTGTC 
AAGGACTCCT CTGCAGAACC ATTATTTCTT TCCATCCTGC AGCATCTTCT TCTAATTAGA 
AATGATTATG ATGCCAGGCC GCAGTACTAT AAACTGATAG ATGAAGTTAT ATCACAGATA 
ATCCTTCAAA AAAATGGGGC TGATCCAGAT TTCAAATGCA AGCATGTAAA CATAGAAATC 
GAAAATCTGA TAGATCATTT TATCGACAAA AAGAAAGTAG AAACAAGCGA GTCAAAAGCA 
CGGGATCTGG TGAAAAAGTT GGACCTAGAG CTTACCGCAC GCCACGAACT CCAGGTGGAA 
ATGAAAGCCA AAGAGAGCGA CTTTGAGCTG AAAATAACAG AGCTGCAGGG GGAGAAGGAA 
TCTTTAGATG CAGAGAAGCA AGAAATCACT GCTGACAAGC AAAAAATGGA GACAGAACTC 
TCCCAACTTG TAGAGCAGGT TGAAAAATTG TCAAAGGAAC TTGAAGCTAC CAAAAAAGAA 
ATGGCATCAA TTTCCACATC TGCTGTTTTA ATCCCTGTAC CTTCTGCCTC TGGAAATGTG 
CCACCTCCAC CACCCCCTCC TCCTCCACTT CCAGGTGCAA CTGGTATTCC TCCACCTCCC 
CCACTTCCAG GTGGCTCTGG TATTCCTCCA CCACCCCCAC TTCCAGGTGG CTCTGGTATT 
GCTCCACCAC TTCCGGCTGG CTTTGGTATT CCTCCACCTC CCCCACTTCC GGGTGGCTTT 
GGTATTCCTC CACCTCCACC ACTTCCCGGT GGCTCTGGTA TTCCTCCACC TCCCCCACTT 
CCGGGTGGCT CTGGTATTCC TCCACCTCCC CCACTTCCGG GTGGCTCTGG TATTCCTCCA 
CCTCCCCCAC TTCCGGGTGG CTCTGGTATT CCTCCCCCAC CACCATTTCC GGGTGGCCCT 
GGAATTCCTC CCCCACCGCC ATTTCCAGGA GGCTTTGGAA TTCCTCCTCC TCCTCCTCCA 
TTTCCAGGTG GCCCTGGAAT TCCTCCTCCT CCTCCATTCC CTGGTGGCCC TGGGGTTCCA 
CCTCCACCTC CAGCATTTGG AGGTGGTGTT CCTCCACCAC CAGGACTTGG AGGCTGGGGG 
GCACCACCAC CCTTACCATT TGGCTTGGCT CCTAAGAAGA ATTATAAGCC AGAAGTGCAA 
CTGAAGCGAC CCAACTGGTC AAAGATCACT CCTGCTGAGC TTTCAGAGAA ATCGTTTTGG 
ACTCAGACAA AAGAAGATCG TTTTGAAAAA GAAGAGCTGT TTGCAAAAAT AACCCTCACT 
TTTTCTTCAC AGATCAAGTC TTCTAAAGCA AAAAAAGCAG AAGATGGTGA TGACAAAAAA 
GCAGCCCAGA AGAAAAAGAC AAAGGAGCTC AAAGTCTTGG ATTCCAAGAC AGCCCAAAAC 
CTATCAATCT TCCTTGGGTC GTTCCGCATG CCATACGAAG AGATCAAAAA TGTGATCCTA 
CAGGTCAATG AGGAAGTGCT GACTGAATCT ATGGTTTCAA ATCTGATCAA GCAGTTACCA 
GAACCAGATC AGTTACAGAT GTTAACAGAG TTCAAGAATG AGTACAAGGA TTTGGCAGAA 
CCAGAGCAGT TTGGAGTAGT GATGTGCAGT GTCCCCCGTT TGCGGCCACG ACTAAATGCC 
ATTCTCTTTA AACTTCTTTT TAATGAGCAA ATTGAAAATA TCAAGCCAGA CATTGTGTCA 
GTTACAGCTG CTTGCGAAGA AGTGCAAAAG TGCCAAAGCT TTGCTAGTCT CCTGGAAATC 
ACACTGCTGG TTGGGAACTT CATGAACGCA GGATCAAGGA ACGCTGATGC TTATGGCTTT 
GACATTAGCT TTCTCTGCAA GTTGAAAGAT ACCAAATCAG CGGACCAGAA GACCACACTG 
CTGCATTTCC TTGTTGAGAC TTGTGAGAAT GACTACCCTG ATGTACTGAA GTTTCCAGAT 
GAGATGGTTC ATGTAGAAAA GGCCAGTAGA GTGTCAGCTG AAACATTGCA AAAGAACCTG 
GATCAAATGA AAAAGCAGAT TGCAGACCTT CAAAGGGACA TTGATAGCTT CCCACCAACA 
GAAGATGAGA AGGATAAGTT TGTAGAAAAA ATGACAATAT TTGTCAAAGA AGCTCAAGAA 
CAGTATACTA AGCTTTGCAT GATGCACGAA AACATGCTGT CATTGTTCCA AGATTTGGGG 
AAATACTTTG TGTTTGACCC CAAGAAAACA TCCATTGAAG AGTTCTTCGC AGACCTGCAG 
AATTTCCGGA ACATGTTCTT GCACGCTCTG AAAGAGAACC AGAAGAGACG GGAAGCAGAA 
GAAAAGATGA AAAGAGCCAA ACTTGCCAAA GAGAAGGCAG AGAAGGAACG GTTAGAGAAG 
CAGCAGAAGA AAGAACAACT GATTGATATG AATGCAGAGG GTGACGAAAC TGGCGTAATG 
GACAGCCTGC TTGAAGCTTT GAATTCGGGA GCTGCATTCA GAAGGAAAAG GGGTCCTCGG 
CAAGACTCTC TCCTGTCCCT CAGTGAGGAG GAGGAGCAGG CAGCGGACTT GCGAACAGAA 
AGGCCGGCTG TGCCGTTACC TCACTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012815149.2
CDS	208..3957
Translation	MEPPAGGGGYGGGPPPPSKGNKEKKKPKGQDEGGDGKSKKFTFKRLMVDELERFTSIRMKKEKEKPYPSHRNSTNFPDNSNPLQTLQELEDDVVLQLFEKMLDDMNLNPEKQQPLREKDIMIKREMVSQYLHTSKAGMSKKESSRSALMYIQDLKSGLGDNSLLLCLESLRVSLNNNPVSWVQNFGAEGLSCLLEILKRIQDEQASLRDVRSEHEIIRCLKAFMNNNFGIKAMLGTEDGILLLARAVDRSVPAMMIDSLKLLSALCILPQPEDMHERVLEALTERAEMEEMERFKPLLDGLNIASPVALKVSCMQLINAVISQGEELDYRVHIRSELMRSGLAKLLKELRNTENEDLKVQLGIFDEHSELDSDDLRGRLDDIRIEMDDFSDVFQVLLNTVKDSSAEPLFLSILQHLLLIRNDYDARPQYYKLIDEVISQIILQKNGADPDFKCKHVNIEIENLIDHFIDKKKVETSESKARDLVKKLDLELTARHELQVEMKAKESDFELKITELQGEKESLDAEKQEITADKQKMETELSQLVEQVEKLSKELEATKKEMASISTSAVLIPVPSASGNVPPPPPPPPPLPGATGIPPPPPLPGGSGIPPPPPLPGGSGIAPPLPAGFGIPPPPPLPGGFGIPPPPPLPGGSGIPPPPPLPGGSGIPPPPPLPGGSGIPPPPPLPGGSGIPPPPPFPGGPGIPPPPPFPGGFGIPPPPPPFPGGPGIPPPPPFPGGPGVPPPPPAFGGGVPPPPGLGGWGAPPPLPFGLAPKKNYKPEVQLKRPNWSKITPAELSEKSFWTQTKEDRFEKEELFAKITLTFSSQIKSSKAKKAEDGDDKKAAQKKKTKELKVLDSKTAQNLSIFLGSFRMPYEEIKNVILQVNEEVLTESMVSNLIKQLPEPDQLQMLTEFKNEYKDLAEPEQFGVVMCSVPRLRPRLNAILFKLLFNEQIENIKPDIVSVTAACEEVQKCQSFASLLEITLLVGNFMNAGSRNADAYGFDISFLCKLKDTKSADQKTTLLHFLVETCENDYPDVLKFPDEMVHVEKASRVSAETLQKNLDQMKKQIADLQRDIDSFPPTEDEKDKFVEKMTIFVKEAQEQYTKLCMMHENMLSLFQDLGKYFVFDPKKTSIEEFFADLQNFRNMFLHALKENQKRREAEEKMKRAKLAKEKAEKERLEKQQKKEQLIDMNAEGDETGVMDSLLEALNSGAAFRRKRGPRQDSLLSLSEEEEQAADLRTERPAVPLPHC

Target ORF information:

RefSeq Version	XM_012959695.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis diaphanous related formin 1 (diaph1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012959695.3

ORF Insert Sequence:

ATGGAACCGC CAGCCGGTGG AGGCGGATAT GGAGGGGGAC CCCCGCCACC TTCCAAGGGC 
AACAAGGAGA AGAAGAAGCC GAAGGGCCAA GATGAAGGTG GAGATGGCAA GTCCAAGAAA 
TTTACCTTTA AGCGGCTGAT GGTAGATGAG CTTGAACGAT TTACCAGTAT ACGGATGAAG 
AAAGAAAAGG AAAAGCCTTA TCCATCACAC AGAAACTCCA CCAACTTCCC AGATAACTCA 
AACCCCCTCC AAACCTTGCA GGAACTCGAA GATGATGTTG TGCTTCAGCT GTTTGAAAAA 
ATGCTGGATG ATATGAACCT CAATCCTGAG AAGCAGCAAC CGCTACGAGA AAAGGATATA 
ATGATAAAAA GAGAGATGGT TTCCCAATAT CTTCACACAT CCAAAGCTGG CATGAGCAAA 
AAGGAGAGTT CTCGTTCTGC ATTAATGTAC ATTCAAGACT TAAAATCCGG ACTAGGAGAC 
AATTCCCTCC TTTTGTGCTT AGAGTCACTA AGAGTATCTT TAAATAACAA TCCTGTCAGC 
TGGGTGCAGA ATTTTGGTGC AGAGGGTCTT TCGTGCCTTT TAGAAATTCT CAAAAGAATA 
CAGGATGAGC AAGCATCCTT GCGGGATGTT AGAAGCGAGC ATGAGATCAT CAGATGTCTG 
AAAGCTTTTA TGAATAACAA TTTTGGAATT AAGGCAATGC TGGGCACAGA AGATGGCATT 
TTGCTGTTAG CGAGAGCAGT AGATCGATCT GTCCCTGCAA TGATGATTGA TTCCTTGAAG 
CTGCTTTCTG CCCTGTGCAT CCTGCCACAA CCAGAGGACA TGCATGAGCG TGTTCTAGAA 
GCCCTTACGG AGAGAGCAGA GATGGAGGAA ATGGAACGAT TTAAACCTCT TCTGGATGGG 
TTAAACATTG CAAGTCCTGT GGCCTTGAAG GTGAGCTGTA TGCAGCTAAT CAATGCAGTG 
ATCAGCCAAG GAGAAGAGCT TGATTACAGA GTTCACATCC GAAGTGAATT GATGCGCTCG 
GGACTGGCCA AACTCCTAAA GGAACTACGT AATACAGAAA ATGAAGACCT GAAGGTACAA 
CTTGGTATTT TTGATGAGCA TTCTGAACTT GATTCTGATG ACTTGCGAGG ACGTTTGGAC 
GACATCCGCA TTGAAATGGA TGACTTCAGT GACGTCTTCC AAGTTCTTCT GAACACTGTC 
AAGGACTCCT CTGCAGAACC ATTATTTCTT TCCATCCTGC AGCATCTTCT TCTAATTAGA 
AATGATTATG ATGCCAGGCC GCAGTACTAT AAACTGATAG ATGAAGTTAT ATCACAGATA 
ATCCTTCAAA AAAATGGGGC TGATCCAGAT TTCAAATGCA AGCATGTAAA CATAGAAATC 
GAAAATCTGA TAGATCATTT TATCGACAAA AAGAAAGTAG AAACAAGCGA GTCAAAAGCA 
CGGGATCTGG TGAAAAAGTT GGACCTAGAG CTTACCGCAC GCCACGAACT CCAGGTGGAA 
ATGAAAGCCA AAGAGAGCGA CTTTGAGCTG AAAATAACAG AGCTGCAGGG GGAGAAGGAA 
TCTTTAGATG CAGAGAAGCA AGAAATCACT GCTGACAAGC AAAAAATGGA GACAGAACTC 
TCCCAACTTG TAGAGCAGGT TGAAAAATTG TCAAAGGAAC TTGAAGCTAC CAAAAAAGAA 
ATGGCATCAA TTTCCACATC TGCTGTTTTA ATCCCTGTAC CTTCTGCCTC TGGAAATGTG 
CCACCTCCAC CACCCCCTCC TCCTCCACTT CCAGGTGCAA CTGGTATTCC TCCACCTCCC 
CCACTTCCAG GTGGCTCTGG TATTCCTCCA CCACCCCCAC TTCCAGGTGG CTCTGGTATT 
GCTCCACCAC TTCCGGCTGG CTTTGGTATT CCTCCACCTC CCCCACTTCC GGGTGGCTTT 
GGTATTCCTC CACCTCCACC ACTTCCCGGT GGCTCTGGTA TTCCTCCACC TCCCCCACTT 
CCGGGTGGCT CTGGTATTCC TCCACCTCCC CCACTTCCGG GTGGCTCTGG TATTCCTCCA 
CCTCCCCCAC TTCCGGGTGG CTCTGGTATT CCTCCCCCAC CACCATTTCC GGGTGGCCCT 
GGAATTCCTC CCCCACCGCC ATTTCCAGGA GGCTTTGGAA TTCCTCCTCC TCCTCCTCCA 
TTTCCAGGTG GCCCTGGAAT TCCTCCTCCT CCTCCATTCC CTGGTGGCCC TGGGGTTCCA 
CCTCCACCTC CAGCATTTGG AGGTGGTGTT CCTCCACCAC CAGGACTTGG AGGCTGGGGG 
GCACCACCAC CCTTACCATT TGGCTTGGCT CCTAAGAAGA ATTATAAGCC AGAAGTGCAA 
CTGAAGCGAC CCAACTGGTC AAAGATCACT CCTGCTGAGC TTTCAGAGAA ATCGTTTTGG 
ACTCAGACAA AAGAAGATCG TTTTGAAAAA GAAGAGCTGT TTGCAAAAAT AACCCTCACT 
TTTTCTTCAC AGATCAAGTC TTCTAAAGCA AAAAAAGCAG AAGATGGTGA TGACAAAAAA 
GCAGCCCAGA AGAAAAAGAC AAAGGAGCTC AAAGTCTTGG ATTCCAAGAC AGCCCAAAAC 
CTATCAATCT TCCTTGGGTC GTTCCGCATG CCATACGAAG AGATCAAAAA TGTGATCCTA 
CAGGTCAATG AGGAAGTGCT GACTGAATCT ATGGTTTCAA ATCTGATCAA GCAGTTACCA 
GAACCAGATC AGTTACAGAT GTTAACAGAG TTCAAGAATG AGTACAAGGA TTTGGCAGAA 
CCAGAGCAGT TTGGAGTAGT GATGTGCAGT GTCCCCCGTT TGCGGCCACG ACTAAATGCC 
ATTCTCTTTA AACTTCTTTT TAATGAGCAA ATTGAAAATA TCAAGCCAGA CATTGTGTCA 
GTTACAGCTG CTTGCGAAGA AGTGCAAAAG TGCCAAAGCT TTGCTAGTCT CCTGGAAATC 
ACACTGCTGG TTGGGAACTT CATGAACGCA GGATCAAGGA ACGCTGATGC TTATGGCTTT 
GACATTAGCT TTCTCTGCAA GTTGAAAGAT ACCAAATCAG CGGACCAGAA GACCACACTG 
CTGCATTTCC TTGTTGAGAC TTGTGAGAAT GACTACCCTG ATGTACTGAA GTTTCCAGAT 
GAGATGGTTC ATGTAGAAAA GGCCAGTAGA GTGTCAGCTG AAACATTGCA AAAGAACCTG 
GATCAAATGA AAAAGCAGAT TGCAGACCTT CAAAGGGACA TTGATAGCTT CCCACCAACA 
GAAGATGAGA AGGATAAGTT TGTAGAAAAA ATGACAATAT TTGTCAAAGA AGCTCAAGAA 
CAGTATACTA AGCTTTGCAT GATGCACGAA AACATGCTGT CATTGTTCCA AGATTTGGGG 
AAATACTTTG TGTTTGACCC CAAGAAAACA TCCATTGAAG AGTTCTTCGC AGACCTGCAG 
AATTTCCGGA ACATGTTCTT GCACGCTCTG AAAGAGAACC AGAAGAGACG GGAAGCAGAA 
GAAAAGATGA AAAGAGCCAA ACTTGCCAAA GAGAAGGCAG AGAAGGAACG GTTAGAGAAG 
CAGCAGAAGA AAGAACAACT GATTGATATG AATGCAGAGG GTGACGAAAC TGGCGTAATG 
GACAGCCTGC TTGAAGCTTT GAATTCGGGA GCTGCATTCA GAAGGAAAAG GGGTCCTCGG 
CAAGACTCTC TCCTGTCCCT CAGTGAGGAG GAGGAGCAGG CAGCGGACTT GCGAACAGAA 
AGGCCGGCTG TGCCGTTACC TCACTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

diaph1 ( XM_012959693.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012815147.1 Xenopus tropicalis diaphanous related formin 1 (diaph1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa28573
Clone ID Related Accession (Same CDS sequence)	XM_012959693.2
Accession Version	XM_012959693.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein diaphanous homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959693.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACCGC CAGCCGGTGG AGGCGGATAT GGAGGGGGAC CCCCGCCACC TTCCAAGGGC 
AACAAGGAGA AGAAGAAGCC GAAGGGCCAA GATGAAGGTG GAGATGGCAA GTCCAAGAAA 
TTTACCTTTA AGCGGCTGAT GGTAGATGAG CTTGAACGAT TTACCAGTAT ACGGATGAAG 
AAAGAAAAGG AAAAGCCTTA TCCATCACAC AGAAACTCCA CCAACTTCCC AGATAACTCA 
AACCCCCTCC AAACCTTGCA GGAACTCGAA GATGATGTTG TGCTTCAGCT GTTTGAAAAA 
ATGCTGGATG ATATGAACCT CAATCCTGAG AAGCAGCAAC CGCTACGAGA AAAGGATATA 
ATGATAAAAA GAGAGATGGT TTCCCAATAT CTTCACACAT CCAAAGCTGG CATGAGCAAA 
AAGGAGAGTT CTCGTTCTGC ATTAATGTAC ATTCAAGACT TAAAATCCGG ACTAGGAGAC 
AATTCCCTCC TTTTGTGCTT AGAGTCACTA AGAGTATCTT TAAATAACAA TCCTGTCAGC 
TGGGTGCAGA ATTTTGGTGC AGAGGGTCTT TCGTGCCTTT TAGAAATTCT CAAAAGAATA 
CAGGATGAGC AAGCATCCTT GCGGGATGTT AGAAGCGAGC ATGAGATCAT CAGATGTCTG 
AAAGCTTTTA TGAATAACAA TTTTGGAATT AAGGCAATGC TGGGCACAGA AGATGGCATT 
TTGCTGTTAG CGAGAGCAGT AGATCGATCT GTCCCTGCAA TGATGATTGA TTCCTTGAAG 
CTGCTTTCTG CCCTGTGCAT CCTGCCACAA CCAGAGGACA TGCATGAGCG TGTTCTAGAA 
GCCCTTACGG AGAGAGCAGA GATGGAGGAA ATGGAACGAT TTAAACCTCT TCTGGATGGG 
TTAAACATTG CAAGTCCTGT GGCCTTGAAG GTGAGCTGTA TGCAGCTAAT CAATGCAGTG 
ATCAGCCAAG GAGAAGAGCT TGATTACAGA GTTCACATCC GAAGTGAATT GATGCGCTCG 
GGACTGGCCA AACTCCTAAA GGAACTACGT AATACAGAAA ATGAAGACCT GAAGGTACAA 
CTTGGTATTT TTGATGAGCA TTCTGAACTT GATTCTGATG ACTTGCGAGG ACGTTTGGAC 
GACATCCGCA TTGAAATGGA TGACTTCAGT GACGTCTTCC AAGTTCTTCT GAACACTGTC 
AAGGACTCCT CTGCAGAACC ATTATTTCTT TCCATCCTGC AGCATCTTCT TCTAATTAGA 
AATGATTATG ATGCCAGGCC GCAGTACTAT AAACTGATAG ATGAAGTTAT ATCACAGATA 
ATCCTTCAAA AAAATGGGGC TGATCCAGAT TTCAAATGCA AGCATGTAAA CATAGAAATC 
GAAAATCTGA TAGATCATTT TATCGACAAA AAGAAAGTAG AAACAAGCGA GTCAAAAGCA 
CGGGATCTGG TGAAAAAGTT GGACCTAGAG CTTACCGCAC GCCACGAACT CCAGGTGGAA 
ATGAAAGCCA AAGAGAGCGA CTTTGAGCTG AAAATAACAG AGCTGCAGGG GGAGAAGGAA 
TCTTTAGATG CAGAGAAGCA AGAAATCACT GCTGACAAGC AAAAAATGGA GACAGAACTC 
TCCCAACTTG TAGAGCAGGT TGAAAAATTG TCAAAGGAAC TTGAAGCTAC CAAAAAAGAA 
ATGGCATCAA TTTCCACATC TGCTGTTTTA ATCCCTGTAC CTTCTGCCTC TGGAAATGTG 
CCACCTCCAC CACCCCCTCC TCCTCCACTT CCAGGTGCAA CTGGTATTCC TCCACCCCCC 
CCACTTCCAG GTGGCTCTGG TATTCCTCCA CCTCCCCCAC TTCCAGGTGG CTCTGGTATT 
CCTCCACCAC CCCCACTTCC AGGTGGCTCT GGTATTTCTC CACCACTTCC GGGTGGCTTT 
GGTATTCCTC CACCTCCCCC ACTTCCGGGT GGCTTTGGTA TTCCTCCACC TCCACCACTT 
CCCGGTGGCT CTGGTATTCC TCCACCTCCC CCACTTCCGG GTGGCTCTGG TATTCCTCCA 
CCTCCCCCAC TTCCGGGTGG CTCTGGTATT CCTCCACCTC CCCCACTTCC GGGTGGCTCT 
GGTATTCCTC CCCCACCACC ACTTCCGGGT GGCTCTGGTA TTCCTCCCCC ACCACCATTT 
CCGGGTGGCC CTGGAATTCC TCCCCCACCG CCATTTCCAG GAGGCTTTGG AATTCCTCCT 
CCTCCTCCTC CATTTCCAGG TGGCCCTGGA ATTCCTCCTC CTCCTCCATT CCCTGGTGGC 
CCTGGGGTTC CACCTCCACC TCCAGCATTT GGAGGTGGTG TTCCTCCACC ACCAGGACTT 
GGAGGCTGGG GGGCACCACC ACCCTTACCA TTTGGCTTGG CTCCTAAGAA GAATTATAAG 
CCAGAAGTGC AACTGAAGCG ACCCAACTGG TCAAAGATCA CTCCTGCTGA GCTTTCAGAG 
AAATCGTTTT GGACTCAGAC AAAAGAAGAT CGTTTTGAAA AAGAAGAGCT GTTTGCAAAA 
ATAACCCTCA CTTTTTCTTC ACAGATCAAG TCTTCTAAAG CAAAAAAAGC AGAAGATGGT 
GATGACAAAA AAGCAGCCCA GAAGAAAAAG ACAAAGGAGC TCAAAGTCTT GGATTCCAAG 
ACAGCCCAAA ACCTATCAAT CTTCCTTGGG TCGTTCCGCA TGCCATACGA AGAGATCAAA 
AATGTGATCC TACAGGTCAA TGAGGAAGTG CTGACTGAAT CTATGGTTTC AAATCTGATC 
AAGCAGTTAC CAGAACCAGA TCAGTTACAG ATGTTAACAG AGTTCAAGAA TGAGTACAAG 
GATTTGGCAG AACCAGAGCA GTTTGGAGTA GTGATGTGCA GTGTCCCCCG TTTGCGGCCA 
CGACTAAATG CCATTCTCTT TAAACTTCTT TTTAATGAGC AAATTGAAAA TATCAAGCCA 
GACATTGTGT CAGTTACAGC TGCTTGCGAA GAAGTGCAAA AGTGCCAAAG CTTTGCTAGT 
CTCCTGGAAA TCACACTGCT GGTTGGGAAC TTCATGAACG CAGGATCAAG GAACGCTGAT 
GCTTATGGCT TTGACATTAG CTTTCTCTGC AAGTTGAAAG ATACCAAATC AGCGGACCAG 
AAGACCACAC TGCTGCATTT CCTTGTTGAG ACTTGTGAGA ATGACTACCC TGATGTACTG 
AAGTTTCCAG ATGAGATGGT TCATGTAGAA AAGGCCAGTA GAGTGTCAGC TGAAACATTG 
CAAAAGAACC TGGATCAAAT GAAAAAGCAG ATTGCAGACC TTCAAAGGGA CATTGATAGC 
TTCCCACCAA CAGAAGATGA GAAGGATAAG TTTGTAGAAA AAATGACAAT ATTTGTCAAA 
GAAGCTCAAG AACAGTATAC TAAGCTTTGC ATGATGCACG AAAACATGCT GTCATTGTTC 
CAAGATTTGG GGAAATACTT TGTGTTTGAC CCCAAGAAAA CATCCATTGA AGAGTTCTTC 
GCAGACCTGC AGAATTTCCG GAACATGTTC TTGCACGCTC TGAAAGAGAA CCAGAAGAGA 
CGGGAAGCAG AAGAAAAGAT GAAAAGAGCC AAACTTGCCA AAGAGAAGGC AGAGAAGGAA 
CGGTTAGAGA AGCAGCAGAA GAAAGAACAA CTGATTGATA TGAATGCAGA GGGTGACGAA 
ACTGGCGTAA TGGACAGCCT GCTTGAAGCT TTGAATTCGG GAGCTGCATT CAGAAGGAAA 
AGGGGTCCTC GGCAAGCGAA CAGAAAGGCC GGCTGTGCCG TTACCTCACT GCTAGCATCT 
GAGCTCACTA AAGAAGATGC AATGACCTCA GTGCCCACTA AGAAGAAAAA ACCCGCTGAG 
ACGGATTTGA TGGAAACAGA TGTAGGACTG CACGAAGATG CCCAGGATAT GCTTACGCGA 
GCAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012815147.1
CDS	575..4483
Translation	MEPPAGGGGYGGGPPPPSKGNKEKKKPKGQDEGGDGKSKKFTFKRLMVDELERFTSIRMKKEKEKPYPSHRNSTNFPDNSNPLQTLQELEDDVVLQLFEKMLDDMNLNPEKQQPLREKDIMIKREMVSQYLHTSKAGMSKKESSRSALMYIQDLKSGLGDNSLLLCLESLRVSLNNNPVSWVQNFGAEGLSCLLEILKRIQDEQASLRDVRSEHEIIRCLKAFMNNNFGIKAMLGTEDGILLLARAVDRSVPAMMIDSLKLLSALCILPQPEDMHERVLEALTERAEMEEMERFKPLLDGLNIASPVALKVSCMQLINAVISQGEELDYRVHIRSELMRSGLAKLLKELRNTENEDLKVQLGIFDEHSELDSDDLRGRLDDIRIEMDDFSDVFQVLLNTVKDSSAEPLFLSILQHLLLIRNDYDARPQYYKLIDEVISQIILQKNGADPDFKCKHVNIEIENLIDHFIDKKKVETSESKARDLVKKLDLELTARHELQVEMKAKESDFELKITELQGEKESLDAEKQEITADKQKMETELSQLVEQVEKLSKELEATKKEMASISTSAVLIPVPSASGNVPPPPPPPPPLPGATGIPPPPPLPGGSGIPPPPPLPGGSGIPPPPPLPGGSGISPPLPGGFGIPPPPPLPGGFGIPPPPPLPGGSGIPPPPPLPGGSGIPPPPPLPGGSGIPPPPPLPGGSGIPPPPPLPGGSGIPPPPPFPGGPGIPPPPPFPGGFGIPPPPPPFPGGPGIPPPPPFPGGPGVPPPPPAFGGGVPPPPGLGGWGAPPPLPFGLAPKKNYKPEVQLKRPNWSKITPAELSEKSFWTQTKEDRFEKEELFAKITLTFSSQIKSSKAKKAEDGDDKKAAQKKKTKELKVLDSKTAQNLSIFLGSFRMPYEEIKNVILQVNEEVLTESMVSNLIKQLPEPDQLQMLTEFKNEYKDLAEPEQFGVVMCSVPRLRPRLNAILFKLLFNEQIENIKPDIVSVTAACEEVQKCQSFASLLEITLLVGNFMNAGSRNADAYGFDISFLCKLKDTKSADQKTTLLHFLVETCENDYPDVLKFPDEMVHVEKASRVSAETLQKNLDQMKKQIADLQRDIDSFPPTEDEKDKFVEKMTIFVKEAQEQYTKLCMMHENMLSLFQDLGKYFVFDPKKTSIEEFFADLQNFRNMFLHALKENQKRREAEEKMKRAKLAKEKAEKERLEKQQKKEQLIDMNAEGDETGVMDSLLEALNSGAAFRRKRGPRQANRKAGCAVTSLLASELTKEDAMTSVPTKKKKPAETDLMETDVGLHEDAQDMLTRAS

Target ORF information:

RefSeq Version	XM_012959693.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis diaphanous related formin 1 (diaph1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012959693.2

ORF Insert Sequence:

ATGGAACCGC CAGCCGGTGG AGGCGGATAT GGAGGGGGAC CCCCGCCACC TTCCAAGGGC 
AACAAGGAGA AGAAGAAGCC GAAGGGCCAA GATGAAGGTG GAGATGGCAA GTCCAAGAAA 
TTTACCTTTA AGCGGCTGAT GGTAGATGAG CTTGAACGAT TTACCAGTAT ACGGATGAAG 
AAAGAAAAGG AAAAGCCTTA TCCATCACAC AGAAACTCCA CCAACTTCCC AGATAACTCA 
AACCCCCTCC AAACCTTGCA GGAACTCGAA GATGATGTTG TGCTTCAGCT GTTTGAAAAA 
ATGCTGGATG ATATGAACCT CAATCCTGAG AAGCAGCAAC CGCTACGAGA AAAGGATATA 
ATGATAAAAA GAGAGATGGT TTCCCAATAT CTTCACACAT CCAAAGCTGG CATGAGCAAA 
AAGGAGAGTT CTCGTTCTGC ATTAATGTAC ATTCAAGACT TAAAATCCGG ACTAGGAGAC 
AATTCCCTCC TTTTGTGCTT AGAGTCACTA AGAGTATCTT TAAATAACAA TCCTGTCAGC 
TGGGTGCAGA ATTTTGGTGC AGAGGGTCTT TCGTGCCTTT TAGAAATTCT CAAAAGAATA 
CAGGATGAGC AAGCATCCTT GCGGGATGTT AGAAGCGAGC ATGAGATCAT CAGATGTCTG 
AAAGCTTTTA TGAATAACAA TTTTGGAATT AAGGCAATGC TGGGCACAGA AGATGGCATT 
TTGCTGTTAG CGAGAGCAGT AGATCGATCT GTCCCTGCAA TGATGATTGA TTCCTTGAAG 
CTGCTTTCTG CCCTGTGCAT CCTGCCACAA CCAGAGGACA TGCATGAGCG TGTTCTAGAA 
GCCCTTACGG AGAGAGCAGA GATGGAGGAA ATGGAACGAT TTAAACCTCT TCTGGATGGG 
TTAAACATTG CAAGTCCTGT GGCCTTGAAG GTGAGCTGTA TGCAGCTAAT CAATGCAGTG 
ATCAGCCAAG GAGAAGAGCT TGATTACAGA GTTCACATCC GAAGTGAATT GATGCGCTCG 
GGACTGGCCA AACTCCTAAA GGAACTACGT AATACAGAAA ATGAAGACCT GAAGGTACAA 
CTTGGTATTT TTGATGAGCA TTCTGAACTT GATTCTGATG ACTTGCGAGG ACGTTTGGAC 
GACATCCGCA TTGAAATGGA TGACTTCAGT GACGTCTTCC AAGTTCTTCT GAACACTGTC 
AAGGACTCCT CTGCAGAACC ATTATTTCTT TCCATCCTGC AGCATCTTCT TCTAATTAGA 
AATGATTATG ATGCCAGGCC GCAGTACTAT AAACTGATAG ATGAAGTTAT ATCACAGATA 
ATCCTTCAAA AAAATGGGGC TGATCCAGAT TTCAAATGCA AGCATGTAAA CATAGAAATC 
GAAAATCTGA TAGATCATTT TATCGACAAA AAGAAAGTAG AAACAAGCGA GTCAAAAGCA 
CGGGATCTGG TGAAAAAGTT GGACCTAGAG CTTACCGCAC GCCACGAACT CCAGGTGGAA 
ATGAAAGCCA AAGAGAGCGA CTTTGAGCTG AAAATAACAG AGCTGCAGGG GGAGAAGGAA 
TCTTTAGATG CAGAGAAGCA AGAAATCACT GCTGACAAGC AAAAAATGGA GACAGAACTC 
TCCCAACTTG TAGAGCAGGT TGAAAAATTG TCAAAGGAAC TTGAAGCTAC CAAAAAAGAA 
ATGGCATCAA TTTCCACATC TGCTGTTTTA ATCCCTGTAC CTTCTGCCTC TGGAAATGTG 
CCACCTCCAC CACCCCCTCC TCCTCCACTT CCAGGTGCAA CTGGTATTCC TCCACCCCCC 
CCACTTCCAG GTGGCTCTGG TATTCCTCCA CCTCCCCCAC TTCCAGGTGG CTCTGGTATT 
CCTCCACCAC CCCCACTTCC AGGTGGCTCT GGTATTTCTC CACCACTTCC GGGTGGCTTT 
GGTATTCCTC CACCTCCCCC ACTTCCGGGT GGCTTTGGTA TTCCTCCACC TCCACCACTT 
CCCGGTGGCT CTGGTATTCC TCCACCTCCC CCACTTCCGG GTGGCTCTGG TATTCCTCCA 
CCTCCCCCAC TTCCGGGTGG CTCTGGTATT CCTCCACCTC CCCCACTTCC GGGTGGCTCT 
GGTATTCCTC CCCCACCACC ACTTCCGGGT GGCTCTGGTA TTCCTCCCCC ACCACCATTT 
CCGGGTGGCC CTGGAATTCC TCCCCCACCG CCATTTCCAG GAGGCTTTGG AATTCCTCCT 
CCTCCTCCTC CATTTCCAGG TGGCCCTGGA ATTCCTCCTC CTCCTCCATT CCCTGGTGGC 
CCTGGGGTTC CACCTCCACC TCCAGCATTT GGAGGTGGTG TTCCTCCACC ACCAGGACTT 
GGAGGCTGGG GGGCACCACC ACCCTTACCA TTTGGCTTGG CTCCTAAGAA GAATTATAAG 
CCAGAAGTGC AACTGAAGCG ACCCAACTGG TCAAAGATCA CTCCTGCTGA GCTTTCAGAG 
AAATCGTTTT GGACTCAGAC AAAAGAAGAT CGTTTTGAAA AAGAAGAGCT GTTTGCAAAA 
ATAACCCTCA CTTTTTCTTC ACAGATCAAG TCTTCTAAAG CAAAAAAAGC AGAAGATGGT 
GATGACAAAA AAGCAGCCCA GAAGAAAAAG ACAAAGGAGC TCAAAGTCTT GGATTCCAAG 
ACAGCCCAAA ACCTATCAAT CTTCCTTGGG TCGTTCCGCA TGCCATACGA AGAGATCAAA 
AATGTGATCC TACAGGTCAA TGAGGAAGTG CTGACTGAAT CTATGGTTTC AAATCTGATC 
AAGCAGTTAC CAGAACCAGA TCAGTTACAG ATGTTAACAG AGTTCAAGAA TGAGTACAAG 
GATTTGGCAG AACCAGAGCA GTTTGGAGTA GTGATGTGCA GTGTCCCCCG TTTGCGGCCA 
CGACTAAATG CCATTCTCTT TAAACTTCTT TTTAATGAGC AAATTGAAAA TATCAAGCCA 
GACATTGTGT CAGTTACAGC TGCTTGCGAA GAAGTGCAAA AGTGCCAAAG CTTTGCTAGT 
CTCCTGGAAA TCACACTGCT GGTTGGGAAC TTCATGAACG CAGGATCAAG GAACGCTGAT 
GCTTATGGCT TTGACATTAG CTTTCTCTGC AAGTTGAAAG ATACCAAATC AGCGGACCAG 
AAGACCACAC TGCTGCATTT CCTTGTTGAG ACTTGTGAGA ATGACTACCC TGATGTACTG 
AAGTTTCCAG ATGAGATGGT TCATGTAGAA AAGGCCAGTA GAGTGTCAGC TGAAACATTG 
CAAAAGAACC TGGATCAAAT GAAAAAGCAG ATTGCAGACC TTCAAAGGGA CATTGATAGC 
TTCCCACCAA CAGAAGATGA GAAGGATAAG TTTGTAGAAA AAATGACAAT ATTTGTCAAA 
GAAGCTCAAG AACAGTATAC TAAGCTTTGC ATGATGCACG AAAACATGCT GTCATTGTTC 
CAAGATTTGG GGAAATACTT TGTGTTTGAC CCCAAGAAAA CATCCATTGA AGAGTTCTTC 
GCAGACCTGC AGAATTTCCG GAACATGTTC TTGCACGCTC TGAAAGAGAA CCAGAAGAGA 
CGGGAAGCAG AAGAAAAGAT GAAAAGAGCC AAACTTGCCA AAGAGAAGGC AGAGAAGGAA 
CGGTTAGAGA AGCAGCAGAA GAAAGAACAA CTGATTGATA TGAATGCAGA GGGTGACGAA 
ACTGGCGTAA TGGACAGCCT GCTTGAAGCT TTGAATTCGG GAGCTGCATT CAGAAGGAAA 
AGGGGTCCTC GGCAAGCGAA CAGAAAGGCC GGCTGTGCCG TTACCTCACT GCTAGCATCT 
GAGCTCACTA AAGAAGATGC AATGACCTCA GTGCCCACTA AGAAGAAAAA ACCCGCTGAG 
ACGGATTTGA TGGAAACAGA TGTAGGACTG CACGAAGATG CCCAGGATAT GCTTACGCGA 
GCAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

diaph1 ( XM_012959694.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012815148.1 Xenopus tropicalis diaphanous related formin 1 (diaph1), transcript variant X2, mRNA.

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CloneID	OXa28574
Clone ID Related Accession (Same CDS sequence)	XM_012959694.2
Accession Version	XM_012959694.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3882bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein diaphanous homolog 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959694.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACCGC CAGCCGGTGG AGGCGGATAT GGAGGGGGAC CCCCGCCACC TTCCAAGGGC 
AACAAGGAGA AGAAGAAGCC GAAGGGCCAA GATGAAGGTG GAGATGGCAA GTCCAAGAAA 
TTTCTTGAAC GATTTACCAG TATACGGATG AAGAAAGAAA AGGAAAAGCC TTATCCATCA 
CACAGAAACT CCACCAACTT CCCAGATAAC TCAAACCCCC TCCAAACCTT GCAGGAACTC 
GAAGATGATG TTGTGCTTCA GCTGTTTGAA AAAATGCTGG ATGATATGAA CCTCAATCCT 
GAGAAGCAGC AACCGCTACG AGAAAAGGAT ATAATGATAA AAAGAGAGAT GGTTTCCCAA 
TATCTTCACA CATCCAAAGC TGGCATGAGC AAAAAGGAGA GTTCTCGTTC TGCATTAATG 
TACATTCAAG ACTTAAAATC CGGACTAGGA GACAATTCCC TCCTTTTGTG CTTAGAGTCA 
CTAAGAGTAT CTTTAAATAA CAATCCTGTC AGCTGGGTGC AGAATTTTGG TGCAGAGGGT 
CTTTCGTGCC TTTTAGAAAT TCTCAAAAGA ATACAGGATG AGCAAGCATC CTTGCGGGAT 
GTTAGAAGCG AGCATGAGAT CATCAGATGT CTGAAAGCTT TTATGAATAA CAATTTTGGA 
ATTAAGGCAA TGCTGGGCAC AGAAGATGGC ATTTTGCTGT TAGCGAGAGC AGTAGATCGA 
TCTGTCCCTG CAATGATGAT TGATTCCTTG AAGCTGCTTT CTGCCCTGTG CATCCTGCCA 
CAACCAGAGG ACATGCATGA GCGTGTTCTA GAAGCCCTTA CGGAGAGAGC AGAGATGGAG 
GAAATGGAAC GATTTAAACC TCTTCTGGAT GGGTTAAACA TTGCAAGTCC TGTGGCCTTG 
AAGGTGAGCT GTATGCAGCT AATCAATGCA GTGATCAGCC AAGGAGAAGA GCTTGATTAC 
AGAGTTCACA TCCGAAGTGA ATTGATGCGC TCGGGACTGG CCAAACTCCT AAAGGAACTA 
CGTAATACAG AAAATGAAGA CCTGAAGGTA CAACTTGGTA TTTTTGATGA GCATTCTGAA 
CTTGATTCTG ATGACTTGCG AGGACGTTTG GACGACATCC GCATTGAAAT GGATGACTTC 
AGTGACGTCT TCCAAGTTCT TCTGAACACT GTCAAGGACT CCTCTGCAGA ACCATTATTT 
CTTTCCATCC TGCAGCATCT TCTTCTAATT AGAAATGATT ATGATGCCAG GCCGCAGTAC 
TATAAACTGA TAGATGAAGT TATATCACAG ATAATCCTTC AAAAAAATGG GGCTGATCCA 
GATTTCAAAT GCAAGCATGT AAACATAGAA ATCGAAAATC TGATAGATCA TTTTATCGAC 
AAAAAGAAAG TAGAAACAAG CGAGTCAAAA GCACGGGATC TGGTGAAAAA GTTGGACCTA 
GAGCTTACCG CACGCCACGA ACTCCAGGTG GAAATGAAAG CCAAAGAGAG CGACTTTGAG 
CTGAAAATAA CAGAGCTGCA GGGGGAGAAG GAATCTTTAG ATGCAGAGAA GCAAGAAATC 
ACTGCTGACA AGCAAAAAAT GGAGACAGAA CTCTCCCAAC TTGTAGAGCA GGTTGAAAAA 
TTGTCAAAGG AACTTGAAGC TACCAAAAAA GAAATGGCAT CAATTTCCAC ATCTGCTGTT 
TTAATCCCTG TACCTTCTGC CTCTGGAAAT GTGCCACCTC CACCACCCCC TCCTCCTCCA 
CTTCCAGGTG CAACTGGTAT TCCTCCACCC CCCCCACTTC CAGGTGGCTC TGGTATTCCT 
CCACCTCCCC CACTTCCAGG TGGCTCTGGT ATTCCTCCAC CACCCCCACT TCCAGGTGGC 
TCTGGTATTT CTCCACCACT TCCGGGTGGC TTTGGTATTC CTCCACCTCC CCCACTTCCG 
GGTGGCTTTG GTATTCCTCC ACCTCCACCA CTTCCCGGTG GCTCTGGTAT TCCTCCACCT 
CCCCCACTTC CGGGTGGCTC TGGTATTCCT CCACCTCCCC CACTTCCGGG TGGCTCTGGT 
ATTCCTCCAC CTCCCCCACT TCCGGGTGGC TCTGGTATTC CTCCCCCACC ACCACTTCCG 
GGTGGCTCTG GTATTCCTCC CCCACCACCA TTTCCGGGTG GCCCTGGAAT TCCTCCCCCA 
CCGCCATTTC CAGGAGGCTT TGGAATTCCT CCTCCTCCTC CTCCATTTCC AGGTGGCCCT 
GGAATTCCTC CTCCTCCTCC ATTCCCTGGT GGCCCTGGGG TTCCACCTCC ACCTCCAGCA 
TTTGGAGGTG GTGTTCCTCC ACCACCAGGA CTTGGAGGCT GGGGGGCACC ACCACCCTTA 
CCATTTGGCT TGGCTCCTAA GAAGAATTAT AAGCCAGAAG TGCAACTGAA GCGACCCAAC 
TGGTCAAAGA TCACTCCTGC TGAGCTTTCA GAGAAATCGT TTTGGACTCA GACAAAAGAA 
GATCGTTTTG AAAAAGAAGA GCTGTTTGCA AAAATAACCC TCACTTTTTC TTCACAGATC 
AAGTCTTCTA AAGCAAAAAA AGCAGAAGAT GGTGATGACA AAAAAGCAGC CCAGAAGAAA 
AAGACAAAGG AGCTCAAAGT CTTGGATTCC AAGACAGCCC AAAACCTATC AATCTTCCTT 
GGGTCGTTCC GCATGCCATA CGAAGAGATC AAAAATGTGA TCCTACAGGT CAATGAGGAA 
GTGCTGACTG AATCTATGGT TTCAAATCTG ATCAAGCAGT TACCAGAACC AGATCAGTTA 
CAGATGTTAA CAGAGTTCAA GAATGAGTAC AAGGATTTGG CAGAACCAGA GCAGTTTGGA 
GTAGTGATGT GCAGTGTCCC CCGTTTGCGG CCACGACTAA ATGCCATTCT CTTTAAACTT 
CTTTTTAATG AGCAAATTGA AAATATCAAG CCAGACATTG TGTCAGTTAC AGCTGCTTGC 
GAAGAAGTGC AAAAGTGCCA AAGCTTTGCT AGTCTCCTGG AAATCACACT GCTGGTTGGG 
AACTTCATGA ACGCAGGATC AAGGAACGCT GATGCTTATG GCTTTGACAT TAGCTTTCTC 
TGCAAGTTGA AAGATACCAA ATCAGCGGAC CAGAAGACCA CACTGCTGCA TTTCCTTGTT 
GAGACTTGTG AGAATGACTA CCCTGATGTA CTGAAGTTTC CAGATGAGAT GGTTCATGTA 
GAAAAGGCCA GTAGAGTGTC AGCTGAAACA TTGCAAAAGA ACCTGGATCA AATGAAAAAG 
CAGATTGCAG ACCTTCAAAG GGACATTGAT AGCTTCCCAC CAACAGAAGA TGAGAAGGAT 
AAGTTTGTAG AAAAAATGAC AATATTTGTC AAAGAAGCTC AAGAACAGTA TACTAAGCTT 
TGCATGATGC ACGAAAACAT GCTGTCATTG TTCCAAGATT TGGGGAAATA CTTTGTGTTT 
GACCCCAAGA AAACATCCAT TGAAGAGTTC TTCGCAGACC TGCAGAATTT CCGGAACATG 
TTCTTGCACG CTCTGAAAGA GAACCAGAAG AGACGGGAAG CAGAAGAAAA GATGAAAAGA 
GCCAAACTTG CCAAAGAGAA GGCAGAGAAG GAACGGTTAG AGAAGCAGCA GAAGAAAGAA 
CAACTGATTG ATATGAATGC AGAGGGTGAC GAAACTGGCG TAATGGACAG CCTGCTTGAA 
GCTTTGAATT CGGGAGCTGC ATTCAGAAGG AAAAGGGGTC CTCGGCAAGC GAACAGAAAG 
GCCGGCTGTG CCGTTACCTC ACTGCTAGCA TCTGAGCTCA CTAAAGAAGA TGCAATGACC 
TCAGTGCCCA CTAAGAAGAA AAAACCCGCT GAGACGGATT TGATGGAAAC AGATGTAGGA 
CTGCACGAAG ATGCCCAGGA TATGCTTACG CGAGCAAGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012815148.1
CDS	573..4454
Translation	MEPPAGGGGYGGGPPPPSKGNKEKKKPKGQDEGGDGKSKKFLERFTSIRMKKEKEKPYPSHRNSTNFPDNSNPLQTLQELEDDVVLQLFEKMLDDMNLNPEKQQPLREKDIMIKREMVSQYLHTSKAGMSKKESSRSALMYIQDLKSGLGDNSLLLCLESLRVSLNNNPVSWVQNFGAEGLSCLLEILKRIQDEQASLRDVRSEHEIIRCLKAFMNNNFGIKAMLGTEDGILLLARAVDRSVPAMMIDSLKLLSALCILPQPEDMHERVLEALTERAEMEEMERFKPLLDGLNIASPVALKVSCMQLINAVISQGEELDYRVHIRSELMRSGLAKLLKELRNTENEDLKVQLGIFDEHSELDSDDLRGRLDDIRIEMDDFSDVFQVLLNTVKDSSAEPLFLSILQHLLLIRNDYDARPQYYKLIDEVISQIILQKNGADPDFKCKHVNIEIENLIDHFIDKKKVETSESKARDLVKKLDLELTARHELQVEMKAKESDFELKITELQGEKESLDAEKQEITADKQKMETELSQLVEQVEKLSKELEATKKEMASISTSAVLIPVPSASGNVPPPPPPPPPLPGATGIPPPPPLPGGSGIPPPPPLPGGSGIPPPPPLPGGSGISPPLPGGFGIPPPPPLPGGFGIPPPPPLPGGSGIPPPPPLPGGSGIPPPPPLPGGSGIPPPPPLPGGSGIPPPPPLPGGSGIPPPPPFPGGPGIPPPPPFPGGFGIPPPPPPFPGGPGIPPPPPFPGGPGVPPPPPAFGGGVPPPPGLGGWGAPPPLPFGLAPKKNYKPEVQLKRPNWSKITPAELSEKSFWTQTKEDRFEKEELFAKITLTFSSQIKSSKAKKAEDGDDKKAAQKKKTKELKVLDSKTAQNLSIFLGSFRMPYEEIKNVILQVNEEVLTESMVSNLIKQLPEPDQLQMLTEFKNEYKDLAEPEQFGVVMCSVPRLRPRLNAILFKLLFNEQIENIKPDIVSVTAACEEVQKCQSFASLLEITLLVGNFMNAGSRNADAYGFDISFLCKLKDTKSADQKTTLLHFLVETCENDYPDVLKFPDEMVHVEKASRVSAETLQKNLDQMKKQIADLQRDIDSFPPTEDEKDKFVEKMTIFVKEAQEQYTKLCMMHENMLSLFQDLGKYFVFDPKKTSIEEFFADLQNFRNMFLHALKENQKRREAEEKMKRAKLAKEKAEKERLEKQQKKEQLIDMNAEGDETGVMDSLLEALNSGAAFRRKRGPRQANRKAGCAVTSLLASELTKEDAMTSVPTKKKKPAETDLMETDVGLHEDAQDMLTRAS

Target ORF information:

RefSeq Version	XM_012959694.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis diaphanous related formin 1 (diaph1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012959694.2

ORF Insert Sequence:

ATGGAACCGC CAGCCGGTGG AGGCGGATAT GGAGGGGGAC CCCCGCCACC TTCCAAGGGC 
AACAAGGAGA AGAAGAAGCC GAAGGGCCAA GATGAAGGTG GAGATGGCAA GTCCAAGAAA 
TTTCTTGAAC GATTTACCAG TATACGGATG AAGAAAGAAA AGGAAAAGCC TTATCCATCA 
CACAGAAACT CCACCAACTT CCCAGATAAC TCAAACCCCC TCCAAACCTT GCAGGAACTC 
GAAGATGATG TTGTGCTTCA GCTGTTTGAA AAAATGCTGG ATGATATGAA CCTCAATCCT 
GAGAAGCAGC AACCGCTACG AGAAAAGGAT ATAATGATAA AAAGAGAGAT GGTTTCCCAA 
TATCTTCACA CATCCAAAGC TGGCATGAGC AAAAAGGAGA GTTCTCGTTC TGCATTAATG 
TACATTCAAG ACTTAAAATC CGGACTAGGA GACAATTCCC TCCTTTTGTG CTTAGAGTCA 
CTAAGAGTAT CTTTAAATAA CAATCCTGTC AGCTGGGTGC AGAATTTTGG TGCAGAGGGT 
CTTTCGTGCC TTTTAGAAAT TCTCAAAAGA ATACAGGATG AGCAAGCATC CTTGCGGGAT 
GTTAGAAGCG AGCATGAGAT CATCAGATGT CTGAAAGCTT TTATGAATAA CAATTTTGGA 
ATTAAGGCAA TGCTGGGCAC AGAAGATGGC ATTTTGCTGT TAGCGAGAGC AGTAGATCGA 
TCTGTCCCTG CAATGATGAT TGATTCCTTG AAGCTGCTTT CTGCCCTGTG CATCCTGCCA 
CAACCAGAGG ACATGCATGA GCGTGTTCTA GAAGCCCTTA CGGAGAGAGC AGAGATGGAG 
GAAATGGAAC GATTTAAACC TCTTCTGGAT GGGTTAAACA TTGCAAGTCC TGTGGCCTTG 
AAGGTGAGCT GTATGCAGCT AATCAATGCA GTGATCAGCC AAGGAGAAGA GCTTGATTAC 
AGAGTTCACA TCCGAAGTGA ATTGATGCGC TCGGGACTGG CCAAACTCCT AAAGGAACTA 
CGTAATACAG AAAATGAAGA CCTGAAGGTA CAACTTGGTA TTTTTGATGA GCATTCTGAA 
CTTGATTCTG ATGACTTGCG AGGACGTTTG GACGACATCC GCATTGAAAT GGATGACTTC 
AGTGACGTCT TCCAAGTTCT TCTGAACACT GTCAAGGACT CCTCTGCAGA ACCATTATTT 
CTTTCCATCC TGCAGCATCT TCTTCTAATT AGAAATGATT ATGATGCCAG GCCGCAGTAC 
TATAAACTGA TAGATGAAGT TATATCACAG ATAATCCTTC AAAAAAATGG GGCTGATCCA 
GATTTCAAAT GCAAGCATGT AAACATAGAA ATCGAAAATC TGATAGATCA TTTTATCGAC 
AAAAAGAAAG TAGAAACAAG CGAGTCAAAA GCACGGGATC TGGTGAAAAA GTTGGACCTA 
GAGCTTACCG CACGCCACGA ACTCCAGGTG GAAATGAAAG CCAAAGAGAG CGACTTTGAG 
CTGAAAATAA CAGAGCTGCA GGGGGAGAAG GAATCTTTAG ATGCAGAGAA GCAAGAAATC 
ACTGCTGACA AGCAAAAAAT GGAGACAGAA CTCTCCCAAC TTGTAGAGCA GGTTGAAAAA 
TTGTCAAAGG AACTTGAAGC TACCAAAAAA GAAATGGCAT CAATTTCCAC ATCTGCTGTT 
TTAATCCCTG TACCTTCTGC CTCTGGAAAT GTGCCACCTC CACCACCCCC TCCTCCTCCA 
CTTCCAGGTG CAACTGGTAT TCCTCCACCC CCCCCACTTC CAGGTGGCTC TGGTATTCCT 
CCACCTCCCC CACTTCCAGG TGGCTCTGGT ATTCCTCCAC CACCCCCACT TCCAGGTGGC 
TCTGGTATTT CTCCACCACT TCCGGGTGGC TTTGGTATTC CTCCACCTCC CCCACTTCCG 
GGTGGCTTTG GTATTCCTCC ACCTCCACCA CTTCCCGGTG GCTCTGGTAT TCCTCCACCT 
CCCCCACTTC CGGGTGGCTC TGGTATTCCT CCACCTCCCC CACTTCCGGG TGGCTCTGGT 
ATTCCTCCAC CTCCCCCACT TCCGGGTGGC TCTGGTATTC CTCCCCCACC ACCACTTCCG 
GGTGGCTCTG GTATTCCTCC CCCACCACCA TTTCCGGGTG GCCCTGGAAT TCCTCCCCCA 
CCGCCATTTC CAGGAGGCTT TGGAATTCCT CCTCCTCCTC CTCCATTTCC AGGTGGCCCT 
GGAATTCCTC CTCCTCCTCC ATTCCCTGGT GGCCCTGGGG TTCCACCTCC ACCTCCAGCA 
TTTGGAGGTG GTGTTCCTCC ACCACCAGGA CTTGGAGGCT GGGGGGCACC ACCACCCTTA 
CCATTTGGCT TGGCTCCTAA GAAGAATTAT AAGCCAGAAG TGCAACTGAA GCGACCCAAC 
TGGTCAAAGA TCACTCCTGC TGAGCTTTCA GAGAAATCGT TTTGGACTCA GACAAAAGAA 
GATCGTTTTG AAAAAGAAGA GCTGTTTGCA AAAATAACCC TCACTTTTTC TTCACAGATC 
AAGTCTTCTA AAGCAAAAAA AGCAGAAGAT GGTGATGACA AAAAAGCAGC CCAGAAGAAA 
AAGACAAAGG AGCTCAAAGT CTTGGATTCC AAGACAGCCC AAAACCTATC AATCTTCCTT 
GGGTCGTTCC GCATGCCATA CGAAGAGATC AAAAATGTGA TCCTACAGGT CAATGAGGAA 
GTGCTGACTG AATCTATGGT TTCAAATCTG ATCAAGCAGT TACCAGAACC AGATCAGTTA 
CAGATGTTAA CAGAGTTCAA GAATGAGTAC AAGGATTTGG CAGAACCAGA GCAGTTTGGA 
GTAGTGATGT GCAGTGTCCC CCGTTTGCGG CCACGACTAA ATGCCATTCT CTTTAAACTT 
CTTTTTAATG AGCAAATTGA AAATATCAAG CCAGACATTG TGTCAGTTAC AGCTGCTTGC 
GAAGAAGTGC AAAAGTGCCA AAGCTTTGCT AGTCTCCTGG AAATCACACT GCTGGTTGGG 
AACTTCATGA ACGCAGGATC AAGGAACGCT GATGCTTATG GCTTTGACAT TAGCTTTCTC 
TGCAAGTTGA AAGATACCAA ATCAGCGGAC CAGAAGACCA CACTGCTGCA TTTCCTTGTT 
GAGACTTGTG AGAATGACTA CCCTGATGTA CTGAAGTTTC CAGATGAGAT GGTTCATGTA 
GAAAAGGCCA GTAGAGTGTC AGCTGAAACA TTGCAAAAGA ACCTGGATCA AATGAAAAAG 
CAGATTGCAG ACCTTCAAAG GGACATTGAT AGCTTCCCAC CAACAGAAGA TGAGAAGGAT 
AAGTTTGTAG AAAAAATGAC AATATTTGTC AAAGAAGCTC AAGAACAGTA TACTAAGCTT 
TGCATGATGC ACGAAAACAT GCTGTCATTG TTCCAAGATT TGGGGAAATA CTTTGTGTTT 
GACCCCAAGA AAACATCCAT TGAAGAGTTC TTCGCAGACC TGCAGAATTT CCGGAACATG 
TTCTTGCACG CTCTGAAAGA GAACCAGAAG AGACGGGAAG CAGAAGAAAA GATGAAAAGA 
GCCAAACTTG CCAAAGAGAA GGCAGAGAAG GAACGGTTAG AGAAGCAGCA GAAGAAAGAA 
CAACTGATTG ATATGAATGC AGAGGGTGAC GAAACTGGCG TAATGGACAG CCTGCTTGAA 
GCTTTGAATT CGGGAGCTGC ATTCAGAAGG AAAAGGGGTC CTCGGCAAGC GAACAGAAAG 
GCCGGCTGTG CCGTTACCTC ACTGCTAGCA TCTGAGCTCA CTAAAGAAGA TGCAATGACC 
TCAGTGCCCA CTAAGAAGAA AAAACCCGCT GAGACGGATT TGATGGAAAC AGATGTAGGA 
CTGCACGAAG ATGCCCAGGA TATGCTTACG CGAGCAAGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

diaph1 ( XM_012959695.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012815149.1 Xenopus tropicalis diaphanous related formin 1 (diaph1), transcript variant X3, mRNA.

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CloneID	OXa28575
Clone ID Related Accession (Same CDS sequence)	XM_012959695.2
Accession Version	XM_012959695.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3882bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein diaphanous homolog 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959695.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACCGC CAGCCGGTGG AGGCGGATAT GGAGGGGGAC CCCCGCCACC TTCCAAGGGC 
AACAAGGAGA AGAAGAAGCC GAAGGGCCAA GATGAAGGTG GAGATGGCAA GTCCAAGAAA 
TTTACCTTTA AGCGGCTGAT GGTAGATGAG CTTGAACGAT TTACCAGTAT ACGGATGAAG 
AAAGAAAAGG AAAAGCCTTA TCCATCACAC AGAAACTCCA CCAACTTCCC AGATAACTCA 
AACCCCCTCC AAACCTTGCA GGAACTCGAA GATGATGTTG TGCTTCAGCT GTTTGAAAAA 
ATGCTGGATG ATATGAACCT CAATCCTGAG AAGCAGCAAC CGCTACGAGA AAAGGATATA 
ATGATAAAAA GAGAGATGGT TTCCCAATAT CTTCACACAT CCAAAGCTGG CATGAGCAAA 
AAGGAGAGTT CTCGTTCTGC ATTAATGTAC ATTCAAGACT TAAAATCCGG ACTAGGAGAC 
AATTCCCTCC TTTTGTGCTT AGAGTCACTA AGAGTATCTT TAAATAACAA TCCTGTCAGC 
TGGGTGCAGA ATTTTGGTGC AGAGGGTCTT TCGTGCCTTT TAGAAATTCT CAAAAGAATA 
CAGGATGAGC AAGCATCCTT GCGGGATGTT AGAAGCGAGC ATGAGATCAT CAGATGTCTG 
AAAGCTTTTA TGAATAACAA TTTTGGAATT AAGGCAATGC TGGGCACAGA AGATGGCATT 
TTGCTGTTAG CGAGAGCAGT AGATCGATCT GTCCCTGCAA TGATGATTGA TTCCTTGAAG 
CTGCTTTCTG CCCTGTGCAT CCTGCCACAA CCAGAGGACA TGCATGAGCG TGTTCTAGAA 
GCCCTTACGG AGAGAGCAGA GATGGAGGAA ATGGAACGAT TTAAACCTCT TCTGGATGGG 
TTAAACATTG CAAGTCCTGT GGCCTTGAAG GTGAGCTGTA TGCAGCTAAT CAATGCAGTG 
ATCAGCCAAG GAGAAGAGCT TGATTACAGA GTTCACATCC GAAGTGAATT GATGCGCTCG 
GGACTGGCCA AACTCCTAAA GGAACTACGT AATACAGAAA ATGAAGACCT GAAGGTACAA 
CTTGGTATTT TTGATGAGCA TTCTGAACTT GATTCTGATG ACTTGCGAGG ACGTTTGGAC 
GACATCCGCA TTGAAATGGA TGACTTCAGT GACGTCTTCC AAGTTCTTCT GAACACTGTC 
AAGGACTCCT CTGCAGAACC ATTATTTCTT TCCATCCTGC AGCATCTTCT TCTAATTAGA 
AATGATTATG ATGCCAGGCC GCAGTACTAT AAACTGATAG ATGAAGTTAT ATCACAGATA 
ATCCTTCAAA AAAATGGGGC TGATCCAGAT TTCAAATGCA AGCATGTAAA CATAGAAATC 
GAAAATCTGA TAGATCATTT TATCGACAAA AAGAAAGTAG AAACAAGCGA GTCAAAAGCA 
CGGGATCTGG TGAAAAAGTT GGACCTAGAG CTTACCGCAC GCCACGAACT CCAGGTGGAA 
ATGAAAGCCA AAGAGAGCGA CTTTGAGCTG AAAATAACAG AGCTGCAGGG GGAGAAGGAA 
TCTTTAGATG CAGAGAAGCA AGAAATCACT GCTGACAAGC AAAAAATGGA GACAGAACTC 
TCCCAACTTG TAGAGCAGGT TGAAAAATTG TCAAAGGAAC TTGAAGCTAC CAAAAAAGAA 
ATGGCATCAA TTTCCACATC TGCTGTTTTA ATCCCTGTAC CTTCTGCCTC TGGAAATGTG 
CCACCTCCAC CACCCCCTCC TCCTCCACTT CCAGGTGCAA CTGGTATTCC TCCACCCCCC 
CCACTTCCAG GTGGCTCTGG TATTCCTCCA CCTCCCCCAC TTCCAGGTGG CTCTGGTATT 
CCTCCACCAC CCCCACTTCC AGGTGGCTCT GGTATTTCTC CACCACTTCC GGGTGGCTTT 
GGTATTCCTC CACCTCCCCC ACTTCCGGGT GGCTTTGGTA TTCCTCCACC TCCACCACTT 
CCCGGTGGCT CTGGTATTCC TCCACCTCCC CCACTTCCGG GTGGCTCTGG TATTCCTCCA 
CCTCCCCCAC TTCCGGGTGG CTCTGGTATT CCTCCACCTC CCCCACTTCC GGGTGGCTCT 
GGTATTCCTC CCCCACCACC ACTTCCGGGT GGCTCTGGTA TTCCTCCCCC ACCACCATTT 
CCGGGTGGCC CTGGAATTCC TCCCCCACCG CCATTTCCAG GAGGCTTTGG AATTCCTCCT 
CCTCCTCCTC CATTTCCAGG TGGCCCTGGA ATTCCTCCTC CTCCTCCATT CCCTGGTGGC 
CCTGGGGTTC CACCTCCACC TCCAGCATTT GGAGGTGGTG TTCCTCCACC ACCAGGACTT 
GGAGGCTGGG GGGCACCACC ACCCTTACCA TTTGGCTTGG CTCCTAAGAA GAATTATAAG 
CCAGAAGTGC AACTGAAGCG ACCCAACTGG TCAAAGATCA CTCCTGCTGA GCTTTCAGAG 
AAATCGTTTT GGACTCAGAC AAAAGAAGAT CGTTTTGAAA AAGAAGAGCT GTTTGCAAAA 
ATAACCCTCA CTTTTTCTTC ACAGATCAAG TCTTCTAAAG CAAAAAAAGC AGAAGATGGT 
GATGACAAAA AAGCAGCCCA GAAGAAAAAG ACAAAGGAGC TCAAAGTCTT GGATTCCAAG 
ACAGCCCAAA ACCTATCAAT CTTCCTTGGG TCGTTCCGCA TGCCATACGA AGAGATCAAA 
AATGTGATCC TACAGGTCAA TGAGGAAGTG CTGACTGAAT CTATGGTTTC AAATCTGATC 
AAGCAGTTAC CAGAACCAGA TCAGTTACAG ATGTTAACAG AGTTCAAGAA TGAGTACAAG 
GATTTGGCAG AACCAGAGCA GTTTGGAGTA GTGATGTGCA GTGTCCCCCG TTTGCGGCCA 
CGACTAAATG CCATTCTCTT TAAACTTCTT TTTAATGAGC AAATTGAAAA TATCAAGCCA 
GACATTGTGT CAGTTACAGC TGCTTGCGAA GAAGTGCAAA AGTGCCAAAG CTTTGCTAGT 
CTCCTGGAAA TCACACTGCT GGTTGGGAAC TTCATGAACG CAGGATCAAG GAACGCTGAT 
GCTTATGGCT TTGACATTAG CTTTCTCTGC AAGTTGAAAG ATACCAAATC AGCGGACCAG 
AAGACCACAC TGCTGCATTT CCTTGTTGAG ACTTGTGAGA ATGACTACCC TGATGTACTG 
AAGTTTCCAG ATGAGATGGT TCATGTAGAA AAGGCCAGTA GAGTGTCAGC TGAAACATTG 
CAAAAGAACC TGGATCAAAT GAAAAAGCAG ATTGCAGACC TTCAAAGGGA CATTGATAGC 
TTCCCACCAA CAGAAGATGA GAAGGATAAG TTTGTAGAAA AAATGACAAT ATTTGTCAAA 
GAAGCTCAAG AACAGTATAC TAAGCTTTGC ATGATGCACG AAAACATGCT GTCATTGTTC 
CAAGATTTGG GGAAATACTT TGTGTTTGAC CCCAAGAAAA CATCCATTGA AGAGTTCTTC 
GCAGACCTGC AGAATTTCCG GAACATGTTC TTGCACGCTC TGAAAGAGAA CCAGAAGAGA 
CGGGAAGCAG AAGAAAAGAT GAAAAGAGCC AAACTTGCCA AAGAGAAGGC AGAGAAGGAA 
CGGTTAGAGA AGCAGCAGAA GAAAGAACAA CTGATTGATA TGAATGCAGA GGGTGACGAA 
ACTGGCGTAA TGGACAGCCT GCTTGAAGCT TTGAATTCGG GAGCTGCATT CAGAAGGAAA 
AGGGGTCCTC GGCAAGACTC TCTCCTGTCC CTCAGTGAGG AGGAGGAGCA GGCAGCGGAC 
TTGGTAACTG TTGGGTGCCT TCGCGTGTCA TGTATTTGTG CTAACAACCC CCTTTTAACT 
GCACACATCC GGCATTTCCC TGTTCATTTT ATATTCATTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012815149.1
CDS	208..4089
Translation	MEPPAGGGGYGGGPPPPSKGNKEKKKPKGQDEGGDGKSKKFTFKRLMVDELERFTSIRMKKEKEKPYPSHRNSTNFPDNSNPLQTLQELEDDVVLQLFEKMLDDMNLNPEKQQPLREKDIMIKREMVSQYLHTSKAGMSKKESSRSALMYIQDLKSGLGDNSLLLCLESLRVSLNNNPVSWVQNFGAEGLSCLLEILKRIQDEQASLRDVRSEHEIIRCLKAFMNNNFGIKAMLGTEDGILLLARAVDRSVPAMMIDSLKLLSALCILPQPEDMHERVLEALTERAEMEEMERFKPLLDGLNIASPVALKVSCMQLINAVISQGEELDYRVHIRSELMRSGLAKLLKELRNTENEDLKVQLGIFDEHSELDSDDLRGRLDDIRIEMDDFSDVFQVLLNTVKDSSAEPLFLSILQHLLLIRNDYDARPQYYKLIDEVISQIILQKNGADPDFKCKHVNIEIENLIDHFIDKKKVETSESKARDLVKKLDLELTARHELQVEMKAKESDFELKITELQGEKESLDAEKQEITADKQKMETELSQLVEQVEKLSKELEATKKEMASISTSAVLIPVPSASGNVPPPPPPPPPLPGATGIPPPPPLPGGSGIPPPPPLPGGSGIPPPPPLPGGSGISPPLPGGFGIPPPPPLPGGFGIPPPPPLPGGSGIPPPPPLPGGSGIPPPPPLPGGSGIPPPPPLPGGSGIPPPPPLPGGSGIPPPPPFPGGPGIPPPPPFPGGFGIPPPPPPFPGGPGIPPPPPFPGGPGVPPPPPAFGGGVPPPPGLGGWGAPPPLPFGLAPKKNYKPEVQLKRPNWSKITPAELSEKSFWTQTKEDRFEKEELFAKITLTFSSQIKSSKAKKAEDGDDKKAAQKKKTKELKVLDSKTAQNLSIFLGSFRMPYEEIKNVILQVNEEVLTESMVSNLIKQLPEPDQLQMLTEFKNEYKDLAEPEQFGVVMCSVPRLRPRLNAILFKLLFNEQIENIKPDIVSVTAACEEVQKCQSFASLLEITLLVGNFMNAGSRNADAYGFDISFLCKLKDTKSADQKTTLLHFLVETCENDYPDVLKFPDEMVHVEKASRVSAETLQKNLDQMKKQIADLQRDIDSFPPTEDEKDKFVEKMTIFVKEAQEQYTKLCMMHENMLSLFQDLGKYFVFDPKKTSIEEFFADLQNFRNMFLHALKENQKRREAEEKMKRAKLAKEKAEKERLEKQQKKEQLIDMNAEGDETGVMDSLLEALNSGAAFRRKRGPRQDSLLSLSEEEEQAADLVTVGCLRVSCICANNPLLTAHIRHFPVHFIFI

Target ORF information:

RefSeq Version	XM_012959695.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis diaphanous related formin 1 (diaph1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012959695.2

ORF Insert Sequence:

ATGGAACCGC CAGCCGGTGG AGGCGGATAT GGAGGGGGAC CCCCGCCACC TTCCAAGGGC 
AACAAGGAGA AGAAGAAGCC GAAGGGCCAA GATGAAGGTG GAGATGGCAA GTCCAAGAAA 
TTTACCTTTA AGCGGCTGAT GGTAGATGAG CTTGAACGAT TTACCAGTAT ACGGATGAAG 
AAAGAAAAGG AAAAGCCTTA TCCATCACAC AGAAACTCCA CCAACTTCCC AGATAACTCA 
AACCCCCTCC AAACCTTGCA GGAACTCGAA GATGATGTTG TGCTTCAGCT GTTTGAAAAA 
ATGCTGGATG ATATGAACCT CAATCCTGAG AAGCAGCAAC CGCTACGAGA AAAGGATATA 
ATGATAAAAA GAGAGATGGT TTCCCAATAT CTTCACACAT CCAAAGCTGG CATGAGCAAA 
AAGGAGAGTT CTCGTTCTGC ATTAATGTAC ATTCAAGACT TAAAATCCGG ACTAGGAGAC 
AATTCCCTCC TTTTGTGCTT AGAGTCACTA AGAGTATCTT TAAATAACAA TCCTGTCAGC 
TGGGTGCAGA ATTTTGGTGC AGAGGGTCTT TCGTGCCTTT TAGAAATTCT CAAAAGAATA 
CAGGATGAGC AAGCATCCTT GCGGGATGTT AGAAGCGAGC ATGAGATCAT CAGATGTCTG 
AAAGCTTTTA TGAATAACAA TTTTGGAATT AAGGCAATGC TGGGCACAGA AGATGGCATT 
TTGCTGTTAG CGAGAGCAGT AGATCGATCT GTCCCTGCAA TGATGATTGA TTCCTTGAAG 
CTGCTTTCTG CCCTGTGCAT CCTGCCACAA CCAGAGGACA TGCATGAGCG TGTTCTAGAA 
GCCCTTACGG AGAGAGCAGA GATGGAGGAA ATGGAACGAT TTAAACCTCT TCTGGATGGG 
TTAAACATTG CAAGTCCTGT GGCCTTGAAG GTGAGCTGTA TGCAGCTAAT CAATGCAGTG 
ATCAGCCAAG GAGAAGAGCT TGATTACAGA GTTCACATCC GAAGTGAATT GATGCGCTCG 
GGACTGGCCA AACTCCTAAA GGAACTACGT AATACAGAAA ATGAAGACCT GAAGGTACAA 
CTTGGTATTT TTGATGAGCA TTCTGAACTT GATTCTGATG ACTTGCGAGG ACGTTTGGAC 
GACATCCGCA TTGAAATGGA TGACTTCAGT GACGTCTTCC AAGTTCTTCT GAACACTGTC 
AAGGACTCCT CTGCAGAACC ATTATTTCTT TCCATCCTGC AGCATCTTCT TCTAATTAGA 
AATGATTATG ATGCCAGGCC GCAGTACTAT AAACTGATAG ATGAAGTTAT ATCACAGATA 
ATCCTTCAAA AAAATGGGGC TGATCCAGAT TTCAAATGCA AGCATGTAAA CATAGAAATC 
GAAAATCTGA TAGATCATTT TATCGACAAA AAGAAAGTAG AAACAAGCGA GTCAAAAGCA 
CGGGATCTGG TGAAAAAGTT GGACCTAGAG CTTACCGCAC GCCACGAACT CCAGGTGGAA 
ATGAAAGCCA AAGAGAGCGA CTTTGAGCTG AAAATAACAG AGCTGCAGGG GGAGAAGGAA 
TCTTTAGATG CAGAGAAGCA AGAAATCACT GCTGACAAGC AAAAAATGGA GACAGAACTC 
TCCCAACTTG TAGAGCAGGT TGAAAAATTG TCAAAGGAAC TTGAAGCTAC CAAAAAAGAA 
ATGGCATCAA TTTCCACATC TGCTGTTTTA ATCCCTGTAC CTTCTGCCTC TGGAAATGTG 
CCACCTCCAC CACCCCCTCC TCCTCCACTT CCAGGTGCAA CTGGTATTCC TCCACCCCCC 
CCACTTCCAG GTGGCTCTGG TATTCCTCCA CCTCCCCCAC TTCCAGGTGG CTCTGGTATT 
CCTCCACCAC CCCCACTTCC AGGTGGCTCT GGTATTTCTC CACCACTTCC GGGTGGCTTT 
GGTATTCCTC CACCTCCCCC ACTTCCGGGT GGCTTTGGTA TTCCTCCACC TCCACCACTT 
CCCGGTGGCT CTGGTATTCC TCCACCTCCC CCACTTCCGG GTGGCTCTGG TATTCCTCCA 
CCTCCCCCAC TTCCGGGTGG CTCTGGTATT CCTCCACCTC CCCCACTTCC GGGTGGCTCT 
GGTATTCCTC CCCCACCACC ACTTCCGGGT GGCTCTGGTA TTCCTCCCCC ACCACCATTT 
CCGGGTGGCC CTGGAATTCC TCCCCCACCG CCATTTCCAG GAGGCTTTGG AATTCCTCCT 
CCTCCTCCTC CATTTCCAGG TGGCCCTGGA ATTCCTCCTC CTCCTCCATT CCCTGGTGGC 
CCTGGGGTTC CACCTCCACC TCCAGCATTT GGAGGTGGTG TTCCTCCACC ACCAGGACTT 
GGAGGCTGGG GGGCACCACC ACCCTTACCA TTTGGCTTGG CTCCTAAGAA GAATTATAAG 
CCAGAAGTGC AACTGAAGCG ACCCAACTGG TCAAAGATCA CTCCTGCTGA GCTTTCAGAG 
AAATCGTTTT GGACTCAGAC AAAAGAAGAT CGTTTTGAAA AAGAAGAGCT GTTTGCAAAA 
ATAACCCTCA CTTTTTCTTC ACAGATCAAG TCTTCTAAAG CAAAAAAAGC AGAAGATGGT 
GATGACAAAA AAGCAGCCCA GAAGAAAAAG ACAAAGGAGC TCAAAGTCTT GGATTCCAAG 
ACAGCCCAAA ACCTATCAAT CTTCCTTGGG TCGTTCCGCA TGCCATACGA AGAGATCAAA 
AATGTGATCC TACAGGTCAA TGAGGAAGTG CTGACTGAAT CTATGGTTTC AAATCTGATC 
AAGCAGTTAC CAGAACCAGA TCAGTTACAG ATGTTAACAG AGTTCAAGAA TGAGTACAAG 
GATTTGGCAG AACCAGAGCA GTTTGGAGTA GTGATGTGCA GTGTCCCCCG TTTGCGGCCA 
CGACTAAATG CCATTCTCTT TAAACTTCTT TTTAATGAGC AAATTGAAAA TATCAAGCCA 
GACATTGTGT CAGTTACAGC TGCTTGCGAA GAAGTGCAAA AGTGCCAAAG CTTTGCTAGT 
CTCCTGGAAA TCACACTGCT GGTTGGGAAC TTCATGAACG CAGGATCAAG GAACGCTGAT 
GCTTATGGCT TTGACATTAG CTTTCTCTGC AAGTTGAAAG ATACCAAATC AGCGGACCAG 
AAGACCACAC TGCTGCATTT CCTTGTTGAG ACTTGTGAGA ATGACTACCC TGATGTACTG 
AAGTTTCCAG ATGAGATGGT TCATGTAGAA AAGGCCAGTA GAGTGTCAGC TGAAACATTG 
CAAAAGAACC TGGATCAAAT GAAAAAGCAG ATTGCAGACC TTCAAAGGGA CATTGATAGC 
TTCCCACCAA CAGAAGATGA GAAGGATAAG TTTGTAGAAA AAATGACAAT ATTTGTCAAA 
GAAGCTCAAG AACAGTATAC TAAGCTTTGC ATGATGCACG AAAACATGCT GTCATTGTTC 
CAAGATTTGG GGAAATACTT TGTGTTTGAC CCCAAGAAAA CATCCATTGA AGAGTTCTTC 
GCAGACCTGC AGAATTTCCG GAACATGTTC TTGCACGCTC TGAAAGAGAA CCAGAAGAGA 
CGGGAAGCAG AAGAAAAGAT GAAAAGAGCC AAACTTGCCA AAGAGAAGGC AGAGAAGGAA 
CGGTTAGAGA AGCAGCAGAA GAAAGAACAA CTGATTGATA TGAATGCAGA GGGTGACGAA 
ACTGGCGTAA TGGACAGCCT GCTTGAAGCT TTGAATTCGG GAGCTGCATT CAGAAGGAAA 
AGGGGTCCTC GGCAAGACTC TCTCCTGTCC CTCAGTGAGG AGGAGGAGCA GGCAGCGGAC 
TTGGTAACTG TTGGGTGCCT TCGCGTGTCA TGTATTTGTG CTAACAACCC CCTTTTAACT 
GCACACATCC GGCATTTCCC TGTTCATTTT ATATTCATTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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diaph1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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