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In vitro cytotoxicity of L-amino acid oxidase from the venom of Crotalus mitchellii pyrrhus.

Toxicon.. 2017-12; 
Tan KK, Ler SG, Gunaratne J, Bay BH, Ponnampalam G.
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Proteins, Expression, Isolation and Analysis .. Cell Proliferation Assay MTS (G3580) was Promega (USA). Caspase-3 (L00289) and Caspase-9 (L00304) colorimetric assay were from GenScript (Piscataway, NJ, USA). ... Caspase activity was assessed using the colorimetric assay kit from GenScript. ... Get A Quote


L-amino acid oxidase isolated from Crotalus mitchellii pyrrhus (Cmp-LAAO) exhibited cytotoxicity against LNCaP prostate adenocarcinoma cells. The viability of LNCaP cells decreased in a concentration- and time-dependent manner upon administration of Cmp-LAAO. Cmp-LAAO induced apoptosis as evidenced by AnnexinV/PI staining using flow cytometry. An increase in caspase-9 and caspase-3 activity were also observed. The damaging effect of LAAO appears to be due to its enzymatic activity, that produces hydrogen peroxide which can then induce oxidative stress within the cells. As expected, the level of oxidative stress in LNCaP cells increased with Cmp-LAAO treatment as confirmed by 2', 7'-dichlorofluorescin diacetate ... More


Apoptosis; Crotalus mitchellii pyrrhus; L-amino acid oxidase; Oxidative stress; Prostate cancer