List by Alphabet: A B C D E F G H I J K L M N O P Q R S T U V W X Y Z
boomerang DNA amplification (BDA)
A technique similar to PCR that also uses a heat-resistant polymerase and cycles of polymerization, denaturation and annealing, but which requires only one primer. The source DNA is digested with a restriction endonuclease. A universal adaptor is engineered with self-complementary sections, so that it loops back upon itself and has ends that permit ligation to both strands at each end of the restriction fragment. In the first amplification cycle a primer anneals to an internal site and the polymerase copies the primer-binding strand through the adaptor, back along the second strand and past the site that is complementary to the primer-binding site of the first strand. In subsequent cycles the primer will have two sites for initiation of polymerization. Hengen, P.N. (1995) Trends Biochem. Sci. 20, 372-373
- Tags:
- Molecular Biology
Related Biology Tools
-
GenSmart™ Codon Optimization
GenSmart Optimization is a free online tool for performing codon optimization to improve gene expression. GenScript's patented algorithms are integrated into the tool to optimize the computing capability of high-performance sequence generation.
-
DNA Construct Design Tool
GenSmart™ Design is a free online DNA construct design tool developed by GenScript. GenSmart™ Design has two design modules, the Create Construct module for individual plasmid design and the Create Library module for DNA library design.
-
Codon Frequency Tables
This online tool shows commonly used genetic codon frequency table in expression host organisms including Escherichia coli and other common host organisms.
Service and Products
Gene Synthesis
Offer gene synthesis of 100% sequence guarantee, free codon optimization, DNA synthesis in as fast as 4 days.
ORF cDNA Clones and Custom Clones
Largest commercial ORF clones database, 70,000 next-day shipping ORF clones.
Plasmid DNA Preparation
High quality plasmids supported by fully-automated platform, 100% full insert sequence accuracy guaranteed.
Express Cloning & Subcloning
and 2 day turnaround, choose from over 150 IP FREE vectors.
Mutation and Library Services
Mutagenesis Portfolio offers several mutation and library services to meet various customers’ needs.
Oligo Synthesis
Bridge the gap between chemically synthesized oligos and bological applications.
-
Top Search
-
Hot Glossary
-
Antibody
If you know of any terms that have been omitted from this glossary that you feel would be useful to include, please send detail to the Editorial Office at GenScript: [email protected]
