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gene splicing

Gene splicing refers to the post-transcriptional processes leading to the removal (i.e., introns) and inclusion (i.e., exons) of sequences in the pre-mRNA, which ultimately shape the final protein-coding mRNA. Splicing reactions are controlled by the spliceosome, a large nuclear ribonucleoprotein complex (RNP) that catalyzes the removal of intron sequences from the pre-mRNA. Alternative splicing gives rise to protein variants or isoforms beyond the wild type protein and is, therefore, a source of diversity.

Although alternative splicing may occur through different mechanisms, exon skipping is the most frequent in eukaryotic cells. Mis-splicing is implicated in various disease states such as cancer, neurodegeneration, and muscular dystrophies.

Gene splicing also refers to molecular cloning techniques used in generating recombinant DNA constructs. For example, the coding regions of a gene may be pieced together into a vector (e.g., plasmid) for delivery into cells of choice (e.g., CHO cells) to drive protein expression.

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Service and Products

Gene Synthesis

Offer gene synthesis of 100% sequence guarantee, free codon optimization, DNA synthesis in as fast as 4 days.

ORF cDNA Clones and Custom Clones

Largest commercial ORF clones database, 70,000 next-day shipping ORF clones.

Plasmid DNA Preparation

High quality plasmids supported by fully-automated platform, 100% full insert sequence accuracy guaranteed.

Express Cloning & Subcloning

$49 and 2 day turnaround, choose from over 150 IP FREE vectors.

Mutation and Library Services

Mutagenesis Portfolio offers several mutation and library services to meet various customers’ needs.

Oligo Synthesis

Bridge the gap between chemically synthesized oligos and bological applications.

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