Revolutionizing Protein Discovery:
Cell Free Meets AI

5 Business Days till Results

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Home » Protein Expression » Cell Free Protein Expression

Overview

In a market with few specialized providers, GenScript’s cell free protein synthesis platform empowers AI-driven protein discovery by offering a competitive edge. While in-house protein expression often incurs high costs and operational bottlenecks, our end-to-end services deliver:

* Compared to conventional methods, based on average cost from open source and customer feedback.

As Fast As
5 Business Days
from sequence to protein

Screen 2-3x More Candidates
with the same budget*

Large Capacity Optimized for HTP Screening
1000+ samples per day

Protein Services

Cell Free Expression Workflows

As fast as 5 Business Days

As fast as 6 Business Days

Sequence analysis and
gene synthesis

Cell free protein
synthesis

Protein purification
and QC

BLI/SPR screening

AI-powered Codon
Optimization
Better yield performance
Plasmid 100% Accuracy

Optimized Production
Process
Proprietary backbone
Proprietary buffer
within 5 Hours

Large Capacity
1000+ samples per day

BLI/SPR
Bundled BLI HT
600 samples per day

Created in https://BioRender.com

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Service Specification

Cell Free Expression Services

Format	Volume	Timeline¹	Deliverables
VHH - His	1 ml (Target yield 10-200 μg)	5 Business Days	Target Protein, CoA Report (A280, SDS-PAGE)
VHH - His	5 ml (Target yield 100-1000 μg)	5 Business Days
Other format	1/5 mL	Pre-evaluation

1. Timeline varies based on sample size.

Cell Free & BLI HT Binding Services

Volume

Format	Volume	Timeline²	Deliverables
VHH-His¹	1mL Supernatant	6 Business Days	BLI Binding Report

1. Other formats needs pre-evaluation.
2. Timeline varies based on sample size.

Case Studies

Case1 VHH-His – VHH-His expressed in cell-free system has similar binding activity as E.coli-based and CHO-based system

Multiple concentration BLI for binding activity test shows that VHH-His expressed in cell-free system has similar binding activity as E.coli-based and CHO-based system.

C423 (E.coli Expressed)

37 VHH-His 1mL Expression *AI generated sequences

C423 (Cell Free Expressed)

107 Mini Protein 1mL Expression *AI generated sequences

C423 (CHO Expressed)

Ligand	Analyte	Chi² (RU²)	ka (1/Ms)	kd (1/s)	KD (M)	Rmax (RU)
C423 (E.coli Expressed)	antigen	3.54E-01	1.32E+05	2.44E-03	1.85E-08	33.0
C423 (Cell Free Expressed)	antigen	1.90E-01	1.35E+05	2.94E-03	2.18E-08	25.3
C423 (CHO Expressed)	antigen	4.33E-01	1.36E+05	2.19E-03	1.61E-08	37.8

Case2 VHH-His – Yield of VHH-His in Genscript’s cell free protein synthesis

Genscript's cell free protein synthesis platform demonstrates robust expression capabilities for VHH-His, achieving average yield >100 mg/L under standardized batch conditions (1/5 mL volume).

VHH-His in 1mL Scale

VHH-His in 5mL Scale
Case3 Miniprotein – Yield of different miniproteins generated by AI in 1 mL scale, with average size of 10 to 20kDa

Genscript‘s cell free protein synthesis platform demonstrates robust expression capabilities for diverse miniproteins generated by AI, achieving average yields exceeding 50 µg in 1 mL volume, which is sufficient for BLI/SPR affinity screening application.
Case4 Enzyme – AI-Driven Restriction Enzyme Optimization via Cell-Free Expression & Custom HTP Screening

Cell free protein synthesis system enables efficient expression of enzymes by eliminating cell viability constraints. Among 92 AI-designed variants of a restriction enzyme, four demonstrated superior protein yields compared to wild-type (WT), with three matching commercial-grade activity while retaining enzymatic fidelity.

Accelerated Timeline: 1 week for cell free enzyme production, 1 week for HTP agarose gel-based activity screening, and 2 weeks for HTP analysis of enzyme-digested substrate fragments. Additional customizable screening methods are available to meet specific project requirements.

Expression of enzyme variants

Enzyme activity screening in agarose gel

Restriction fragment analysis

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Protein Services

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