
As the coronavirus (COVID-19) pandemic continues to evolve globally, many of us have been inevitably impacted in our daily life. At this unprecedented moment, filled with challenges and uncertainty, GenScript is firmly committed to serving our clients, the research and healthcare community, and anyone in need of us, to the best of our abilities.
Using GenScript’s exclusive High Density HD-293 mammalian expression system, we are able to produce gram level proteins, that are proven to work with clinical sample in ELISA assay!
Functional 2019-nCoV Spike protein (RBD) for COVID-19 Research
Name | Application | Purity | QC |
---|---|---|---|
S protein (RBD) His | IgM/IgG kit with Anti-His antibody | >90% | SDS-PAGE, WB, ELISA binding against ACE-2 |
S protein (RBD) mFc | IgM/IgG kit with Anti-Mouse antibody |
Gram level, ready-to-ship RBD proteins are available, talk to your account manager!
SDS-PAGE & Western blot Analysis:

Fig.1 SDS-PAGE and Western blot analysis of RBD Protein (His tag)
Lane M1: Protein Marker, GenScript, Cat. No. M00516
Lane M2: Protein Marker, GenScript, Cat. No. M00521
Lane 1: BSA (2.00 μg)
Lane 2: RBD Protein (Reducing condition, 2.00 μg)
Lane 3: RBD Protein (Non-reducing condition, 2.00 μg)
Lane 4: RBD Protein (Reducing condition)
Lane 5: RBD Protein (Non-reducing condition)
Primary antibody: Mouse-anti-His mAb (GenScript, Cat.No. A00186)
Purity >90%
ELISA Results Binding against ACE2

SDS-PAGE & Western blot Analysis:

Fig.1 SDS-PAGE and Western blot analysis of S-RBD (mFc-tag)
Lane M1: Protein Marker, TaKaRa, Cat. No. 3452
Lane M2: Protein Marker, GenScript, Cat. No. M00521
Lane 1: Reducing condition
Lane 2: Non-reducing condition
Lane P: Mouse IgG1, Kappa (Sigma, Cat.No.M9269) as a positive control
Primary antibody: MonoRab™ Mouse IgG (76F10), mAb, Rabbit (GenScript, Cat.No. Cat. V90301)
Purity >90%
ELISA Results Binding against ACE2

Spike RBD-sc-dimer
Lane M: Merker
Lane 1: Reducing condition
Lane 2: Non-reducing condition
Purity: 90% by SDS-PAGE; 98% by SEC-HPLC

EC50=16.8ng/mL, measured by ELISA against AEC-2
Lianpan Dai, Tianyi Zheng, Kun Xu, A universal design of betacoronavirus vaccines against COVID-19, MERS and SARS.Cell.August,6,2020.
Assay cell line overexpression ACE2
SC1394: ACE2 overexpression cell line |
Cell types | Deliverables | TAT |
---|---|---|---|
Both cell pools and single clones options are available |
HEK293T | 2 vials, 1x10^6Cells/vial | 3-4 weeks |
A549 | |||
CHOK1 | |||
NIH-3T3 |
Case Studies
Anti-Flag antibody to detect ACE2-Flag

S protein to detect ACE2-Flag

Anti-Flag antibody to detect ACE2-Flag

S protein to detect ACE2-Flag

Anti-Flag antibody to detect ACE2-Flag

S protein to detect ACE2-Flag

Quotations and Ordering
