IARS cDNA ORF clone, Ailuropoda melanoleuca(giant panda)

Gene
Clones
gRNAs

Summary

Gene Symbol	IARS
Entrez Gene ID	100470717
Full Name	isoleucyl-tRNA synthetase
Gene Type	protein-coding
Organism	Ailuropoda melanoleuca(giant panda)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011235347.2	XP_011233649.1	isoleucine--tRNA ligase, cytoplasmic isoform X1
XM_019808397.1	XP_019663956.1	isoleucine--tRNA ligase, cytoplasmic isoform X1
XM_019808399.1	XP_019663958.1	isoleucine--tRNA ligase, cytoplasmic isoform X2

Pathways from BioSystems
Gene Ontology

The following IARS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IARS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAc98626	XM_019808399.1 Latest version!	Ailuropoda melanoleuca isoleucyl-tRNA synthetase (IARS), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OAc14733	XM_011235347.2 Latest version!	Ailuropoda melanoleuca isoleucyl-tRNA synthetase (IARS), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OAc14733	XM_019808397.1 Latest version!	Ailuropoda melanoleuca isoleucyl-tRNA synthetase (IARS), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IARS ( XM_019808399.1 ) cDNA ORF clone, Ailuropoda melanoleuca(giant panda) -> XP_019663958.1 Ailuropoda melanoleuca isoleucyl-tRNA synthetase (IARS), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OAc98626
Clone ID Related Accession (Same CDS sequence)	XM_019808399.1
Accession Version	XM_019808399.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-27
Organism	Ailuropoda melanoleuca(giant panda)
Product	isoleucine--tRNA ligase, cytoplasmic isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003218612.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ailuropoda melanoleuca Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTCAGC AAGTTCCAGA AAACATCAAT TTTCCTGCTG AAGAAGAGAA AATCTTACAG 
TTCTGGTCCG AATTTAACTG TTTTCAGGAA TGCTTAAAGC AATCAAAACA GAAGCCAAAA 
TTTACGTTCT ATGATGGGCC TCCTTTTGCA ACTGGACTGC CTCACTATGG ACATATACTT 
GCAGGGACAA TTAAAGATAT AGTTACCAGA TACGCTCACC AGAGTGGGTT TCATGTTGAC 
AGAAGATTTG GATGGGATTG TCATGGCTTA CCTGTGGAAT ATGAAATTGA TAAGACACTG 
GGAATCAGAG GACCAGAGGA TGTAGCCAAA ATGGGCATTG TAGAGTATAA CAATCAGTGC 
CGAGCAATTG TGATGAGATA TTCTACTGAG TGGAAGTCCA CTGTTAACAG ACTTGGCCGA 
TGGATTGACT TTGACAATGA CTACAAAACT CTGTATCCAC AATTCATGGA ATCTGTCTGG 
TGGGTCTTCA AGCAGCTCTA TGATAAAGGC CTTGTGTATA GAGGTGTGAA AGTCATGCCT 
TTCTCCACCG CGTGCAACAC TCCACTTTCC AACTTCGAAT CTCACCAGAA CTACAAGGAT 
GTTCAGGACC CTTCAATATT TGTAACTTTC CCTTTGGAGG AAGATGAAAA TGTGTCCTTG 
GTGGCTTGGA CGACCACTCC TTGGACTCTA CCTAGTAATC TTGCCCTGTG TGTTAATCCG 
GATATGCAGT ACGTGAAGAT TAAAGATGTT GTCAGAGGAA AATTACTCAT TTTAATGGAA 
GCCAGATTAC CAGCCCTCTA TAAGTCGGAG AGTGACTATG AGGTCCTTGA AAGATTTCCT 
GGTAGTCATC TCAAAGGCAA GAAGTACAGG CCCCTGTTTG ACTATTTTGT GCAGTGCAAG 
GAGAATGGTG CTTTCACGGT GCTCGCTGAC AGCTACGTGC GGGAGGAGGA GGGCACGGGA 
GTGGTGCACC AGGCTCCTTA CTTCGGTGCT GACGACTATC GGGTCTGTAT GGACTTGAGC 
ATCATTCAGA AAGACTCTCC TCCTGTGTGC CCTGTGGACG CTTCAGGCTG TTTCACGGCG 
GAGGTGACAG ATTTCACGGG ACAGTATGTG AAGGATGCTG ACAAAAACAT CATCAGGACC 
CTGAAGGAAA AAGGCCGACT TCTTGTCTCC AGCACCTTCA CTCACAGTTA CCCCTTCTGC 
TGGAGGTCAG ACACCCCCCT GATCTACAAA GCGGTGCCCA GCTGGTTCGT GCGGGTGGAG 
CACATGGTGG AGCAGCTCCT GAGGAACAAC GACTTGTGCT ACTGGGTCCC AGAGTTTGTG 
CGAGAAAAGC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGGCAGT TTCCAGAAAC 
AGATACTGGG GCACCCCCAT CCCCCTGTGG GTCAGTGATG ACTTCGAGGA GGTAGTATGC 
ATTGGATCGA TGGCTGAACT TGAAGAACTG TCGGGGGCAA AGGTCTCTGA TCTCCACAGA 
GAGAGCATTG ACCATTTGAC CATCCCTTCC CGCTGCGGGA AGGGAGTGTT GCATCGCATC 
TCTGAAGTGT TTGACTGTTG GTTCGAGAGC GGCAGCATGC CCTATGCTCA GGTTCATTAT 
CCGTTTGAAA ACAAGAGGGA GTTTGAGGAC ACATTTCCTG CAGACTTCAT TGCCGAAGGC 
ATTGACCAGA CCAGAGGATG GTTTTACACC CTGCTGGTGC TGGCCACCGC TCTCTTTGGA 
CAGCCGCCTT TCAGGAACGT CATTGTGAAC GGGCTCGTCC TGGCAAGTGA TGGTCAAAAA 
ATGAGCAAAC GAAAAAAGAA TTACCCGGAT CCACTTTCAA TCATCCATAA ATATGGTGCC 
GACGCCCTCA GATTATATCT GATCAACTCC CCTGTGGTGA AAGCAGAAAA CCTCCGATTT 
AAGGAGGAGG GTGTGCGTGA TGTCTTGAAG GACGTGCTGC TCCCTTGGTA CAATGCCTAC 
CGCTTCTTCA TCCAGAATGT CCTGAGGCTG CAGAAGGAGG AAGGAATGGA ATTTCTCTAC 
AATGAGAACA CAGTTAAAGG AAGTGCCAAC GTCACGGACC GGTGGGTCCT ATCCTGCATA 
CAGTCGCTGG TTGGGTTCTT TGAGACCGAA ATGGCAGCTT ACAGGCTCTA TACGGTGGTG 
CCTCGCCTGG TCAAGTTCGT GGATGTCCTA ACCAACTGGT ACGTCAGGAT GAACCGCAGA 
AGGCTGAAGG GTGAAAACGG GGTGGAGGAC TGTGTTACAG CCCTGGAGAC CTTGTTCAGT 
GTCCTGCTGT CTCTGTGCAG ACTGATGGCA CCCTATACAC CTTTTCTCAC CGAATTGATG 
TACCAGAATC TAAAGCTGCT GATCGACCCT GTTTCTGTCC AGGACAAGGA CACACACAGT 
ATCCACTACC TCATGCTGCC CCATGTTCGG GAGGAGCTGA TTGACAAGAA CACGGAGAGA 
GCAGTGTCTC GGATGCAGTC TGTGATCGAA CTTGGGCGAG TGATTCGAGA CCGCAAAACC 
ATTCCAATAA AGTATCCTTT GAAAGAAATT GTGGTTATCC ATCAGGATCC AGAAGCTCTT 
CATGAGATCA AGTCTTTGGA GAAGTATATT ATTGAGGAAT TAAACGTCCG AAAGGTCACG 
CTGTCTACAG ATAAAAACAA GTATGGCATT CGGCTAAGGG CAGAGCCTGA TCACATGGTC 
CTGGGGAAGC GTCTGAAGGG AGCCTTCAGG GCGGTGATGA CGTCCATCAA GAAGCTGAGC 
AGTGAAGAGC TGGAGCGCTT CCAGGAGAGC GGGACCATTG TTGTAGAAGG CCACGAGCTG 
CATGAAGAGG ACATCCGCCT CATGTACACC TTTGATCAGA CGACAGGAGG GACGACGCAG 
TATGAAGCAC ACTCTGACGC CCAGGTTTTG GTCCTCTTAG ATGTCACCCC TGACCAGTCA 
ATGGTGGATG AAGGAGTGGC TCGGGAGGTC ATCAACCGCA TCCAGAAACT TCGCAAAAAG 
TGCAATCTGG TTCCAACTGA TGAAATAACA GTTTATTATA AAGCGACGTC TGAAGGGAAG 
TATCTGAATA ATGTTATCGA AAGCCACACG GAGTTCATAT TTGCCACCAT AAAGGCTCCC 
TTGAAACCGT ATCCAGTTCC AGTGTCAGAT AAAGTCCTTA TTCAAGAAGA AACACAGTTA 
AAGGGCTCTG ACCTGGAAAT TACCATCACC AGAGGGTCGT CCCTGCCTGG TCCTGCTTGT 
GCGTATGTCA ACCTCAACAT TTGTACAGAT GGCAGTGAGC AAGACAGAAC CAGGAGGGGT 
GCAGCGTGGC AAGAGAAAAT TCGTAGACAG TATCTGTTCT ACTCCAGACA GACAAACACA 
ACAGAAGAAA CCGGCCCCAC TTACTCCCAC CAGCAAAGAC CAAGTGGGGA GCCTAGACCT 
TTTTCAGGCT CCTGCCACCT CACGGTGTCA GAGAAGCCCG CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019663958.1
CDS	38..3502
Translation	MVQQVPENINFPAEEEKILQFWSEFNCFQECLKQSKQKPKFTFYDGPPFATGLPHYGHILAGTIKDIVTRYAHQSGFHVDRRFGWDCHGLPVEYEIDKTLGIRGPEDVAKMGIVEYNNQCRAIVMRYSTEWKSTVNRLGRWIDFDNDYKTLYPQFMESVWWVFKQLYDKGLVYRGVKVMPFSTACNTPLSNFESHQNYKDVQDPSIFVTFPLEEDENVSLVAWTTTPWTLPSNLALCVNPDMQYVKIKDVVRGKLLILMEARLPALYKSESDYEVLERFPGSHLKGKKYRPLFDYFVQCKENGAFTVLADSYVREEEGTGVVHQAPYFGADDYRVCMDLSIIQKDSPPVCPVDASGCFTAEVTDFTGQYVKDADKNIIRTLKEKGRLLVSSTFTHSYPFCWRSDTPLIYKAVPSWFVRVEHMVEQLLRNNDLCYWVPEFVREKRFGNWLKDARDWAVSRNRYWGTPIPLWVSDDFEEVVCIGSMAELEELSGAKVSDLHRESIDHLTIPSRCGKGVLHRISEVFDCWFESGSMPYAQVHYPFENKREFEDTFPADFIAEGIDQTRGWFYTLLVLATALFGQPPFRNVIVNGLVLASDGQKMSKRKKNYPDPLSIIHKYGADALRLYLINSPVVKAENLRFKEEGVRDVLKDVLLPWYNAYRFFIQNVLRLQKEEGMEFLYNENTVKGSANVTDRWVLSCIQSLVGFFETEMAAYRLYTVVPRLVKFVDVLTNWYVRMNRRRLKGENGVEDCVTALETLFSVLLSLCRLMAPYTPFLTELMYQNLKLLIDPVSVQDKDTHSIHYLMLPHVREELIDKNTERAVSRMQSVIELGRVIRDRKTIPIKYPLKEIVVIHQDPEALHEIKSLEKYIIEELNVRKVTLSTDKNKYGIRLRAEPDHMVLGKRLKGAFRAVMTSIKKLSSEELERFQESGTIVVEGHELHEEDIRLMYTFDQTTGGTTQYEAHSDAQVLVLLDVTPDQSMVDEGVAREVINRIQKLRKKCNLVPTDEITVYYKATSEGKYLNNVIESHTEFIFATIKAPLKPYPVPVSDKVLIQEETQLKGSDLEITITRGSSLPGPACAYVNLNICTDGSEQDRTRRGAAWQEKIRRQYLFYSRQTNTTEETGPTYSHQQRPSGEPRPFSGSCHLTVSEKPA

Target ORF information:

RefSeq Version	XM_019808399.1
Organism	Ailuropoda melanoleuca(giant panda)
Definition	Ailuropoda melanoleuca isoleucyl-tRNA synthetase (IARS), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019808399.1

ORF Insert Sequence:

ATGGTTCAGC AAGTTCCAGA AAACATCAAT TTTCCTGCTG AAGAAGAGAA AATCTTACAG 
TTCTGGTCCG AATTTAACTG TTTTCAGGAA TGCTTAAAGC AATCAAAACA GAAGCCAAAA 
TTTACGTTCT ATGATGGGCC TCCTTTTGCA ACTGGACTGC CTCACTATGG ACATATACTT 
GCAGGGACAA TTAAAGATAT AGTTACCAGA TACGCTCACC AGAGTGGGTT TCATGTTGAC 
AGAAGATTTG GATGGGATTG TCATGGCTTA CCTGTGGAAT ATGAAATTGA TAAGACACTG 
GGAATCAGAG GACCAGAGGA TGTAGCCAAA ATGGGCATTG TAGAGTATAA CAATCAGTGC 
CGAGCAATTG TGATGAGATA TTCTACTGAG TGGAAGTCCA CTGTTAACAG ACTTGGCCGA 
TGGATTGACT TTGACAATGA CTACAAAACT CTGTATCCAC AATTCATGGA ATCTGTCTGG 
TGGGTCTTCA AGCAGCTCTA TGATAAAGGC CTTGTGTATA GAGGTGTGAA AGTCATGCCT 
TTCTCCACCG CGTGCAACAC TCCACTTTCC AACTTCGAAT CTCACCAGAA CTACAAGGAT 
GTTCAGGACC CTTCAATATT TGTAACTTTC CCTTTGGAGG AAGATGAAAA TGTGTCCTTG 
GTGGCTTGGA CGACCACTCC TTGGACTCTA CCTAGTAATC TTGCCCTGTG TGTTAATCCG 
GATATGCAGT ACGTGAAGAT TAAAGATGTT GTCAGAGGAA AATTACTCAT TTTAATGGAA 
GCCAGATTAC CAGCCCTCTA TAAGTCGGAG AGTGACTATG AGGTCCTTGA AAGATTTCCT 
GGTAGTCATC TCAAAGGCAA GAAGTACAGG CCCCTGTTTG ACTATTTTGT GCAGTGCAAG 
GAGAATGGTG CTTTCACGGT GCTCGCTGAC AGCTACGTGC GGGAGGAGGA GGGCACGGGA 
GTGGTGCACC AGGCTCCTTA CTTCGGTGCT GACGACTATC GGGTCTGTAT GGACTTGAGC 
ATCATTCAGA AAGACTCTCC TCCTGTGTGC CCTGTGGACG CTTCAGGCTG TTTCACGGCG 
GAGGTGACAG ATTTCACGGG ACAGTATGTG AAGGATGCTG ACAAAAACAT CATCAGGACC 
CTGAAGGAAA AAGGCCGACT TCTTGTCTCC AGCACCTTCA CTCACAGTTA CCCCTTCTGC 
TGGAGGTCAG ACACCCCCCT GATCTACAAA GCGGTGCCCA GCTGGTTCGT GCGGGTGGAG 
CACATGGTGG AGCAGCTCCT GAGGAACAAC GACTTGTGCT ACTGGGTCCC AGAGTTTGTG 
CGAGAAAAGC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGGCAGT TTCCAGAAAC 
AGATACTGGG GCACCCCCAT CCCCCTGTGG GTCAGTGATG ACTTCGAGGA GGTAGTATGC 
ATTGGATCGA TGGCTGAACT TGAAGAACTG TCGGGGGCAA AGGTCTCTGA TCTCCACAGA 
GAGAGCATTG ACCATTTGAC CATCCCTTCC CGCTGCGGGA AGGGAGTGTT GCATCGCATC 
TCTGAAGTGT TTGACTGTTG GTTCGAGAGC GGCAGCATGC CCTATGCTCA GGTTCATTAT 
CCGTTTGAAA ACAAGAGGGA GTTTGAGGAC ACATTTCCTG CAGACTTCAT TGCCGAAGGC 
ATTGACCAGA CCAGAGGATG GTTTTACACC CTGCTGGTGC TGGCCACCGC TCTCTTTGGA 
CAGCCGCCTT TCAGGAACGT CATTGTGAAC GGGCTCGTCC TGGCAAGTGA TGGTCAAAAA 
ATGAGCAAAC GAAAAAAGAA TTACCCGGAT CCACTTTCAA TCATCCATAA ATATGGTGCC 
GACGCCCTCA GATTATATCT GATCAACTCC CCTGTGGTGA AAGCAGAAAA CCTCCGATTT 
AAGGAGGAGG GTGTGCGTGA TGTCTTGAAG GACGTGCTGC TCCCTTGGTA CAATGCCTAC 
CGCTTCTTCA TCCAGAATGT CCTGAGGCTG CAGAAGGAGG AAGGAATGGA ATTTCTCTAC 
AATGAGAACA CAGTTAAAGG AAGTGCCAAC GTCACGGACC GGTGGGTCCT ATCCTGCATA 
CAGTCGCTGG TTGGGTTCTT TGAGACCGAA ATGGCAGCTT ACAGGCTCTA TACGGTGGTG 
CCTCGCCTGG TCAAGTTCGT GGATGTCCTA ACCAACTGGT ACGTCAGGAT GAACCGCAGA 
AGGCTGAAGG GTGAAAACGG GGTGGAGGAC TGTGTTACAG CCCTGGAGAC CTTGTTCAGT 
GTCCTGCTGT CTCTGTGCAG ACTGATGGCA CCCTATACAC CTTTTCTCAC CGAATTGATG 
TACCAGAATC TAAAGCTGCT GATCGACCCT GTTTCTGTCC AGGACAAGGA CACACACAGT 
ATCCACTACC TCATGCTGCC CCATGTTCGG GAGGAGCTGA TTGACAAGAA CACGGAGAGA 
GCAGTGTCTC GGATGCAGTC TGTGATCGAA CTTGGGCGAG TGATTCGAGA CCGCAAAACC 
ATTCCAATAA AGTATCCTTT GAAAGAAATT GTGGTTATCC ATCAGGATCC AGAAGCTCTT 
CATGAGATCA AGTCTTTGGA GAAGTATATT ATTGAGGAAT TAAACGTCCG AAAGGTCACG 
CTGTCTACAG ATAAAAACAA GTATGGCATT CGGCTAAGGG CAGAGCCTGA TCACATGGTC 
CTGGGGAAGC GTCTGAAGGG AGCCTTCAGG GCGGTGATGA CGTCCATCAA GAAGCTGAGC 
AGTGAAGAGC TGGAGCGCTT CCAGGAGAGC GGGACCATTG TTGTAGAAGG CCACGAGCTG 
CATGAAGAGG ACATCCGCCT CATGTACACC TTTGATCAGA CGACAGGAGG GACGACGCAG 
TATGAAGCAC ACTCTGACGC CCAGGTTTTG GTCCTCTTAG ATGTCACCCC TGACCAGTCA 
ATGGTGGATG AAGGAGTGGC TCGGGAGGTC ATCAACCGCA TCCAGAAACT TCGCAAAAAG 
TGCAATCTGG TTCCAACTGA TGAAATAACA GTTTATTATA AAGCGACGTC TGAAGGGAAG 
TATCTGAATA ATGTTATCGA AAGCCACACG GAGTTCATAT TTGCCACCAT AAAGGCTCCC 
TTGAAACCGT ATCCAGTTCC AGTGTCAGAT AAAGTCCTTA TTCAAGAAGA AACACAGTTA 
AAGGGCTCTG ACCTGGAAAT TACCATCACC AGAGGGTCGT CCCTGCCTGG TCCTGCTTGT 
GCGTATGTCA ACCTCAACAT TTGTACAGAT GGCAGTGAGC AAGACAGAAC CAGGAGGGGT 
GCAGCGTGGC AAGAGAAAAT TCGTAGACAG TATCTGTTCT ACTCCAGACA GACAAACACA 
ACAGAAGAAA CCGGCCCCAC TTACTCCCAC CAGCAAAGAC CAAGTGGGGA GCCTAGACCT 
TTTTCAGGCT CCTGCCACCT CACGGTGTCA GAGAAGCCCG CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IARS ( XM_011235347.2 ) cDNA ORF clone, Ailuropoda melanoleuca(giant panda) -> XP_011233649.1 Ailuropoda melanoleuca isoleucyl-tRNA synthetase (IARS), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAc14733
Clone ID Related Accession (Same CDS sequence)	XM_011235347.2 , XM_019808397.1
Accession Version	XM_011235347.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-27
Organism	Ailuropoda melanoleuca(giant panda)
Product	isoleucine--tRNA ligase, cytoplasmic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003218612.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 28, 2016 this sequence version replaced XM_011235347.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ailuropoda melanoleuca Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTCAGC AAGTTCCAGA AAACATCAAT TTTCCTGCTG AAGAAGAGAA AATCTTACAG 
TTCTGGTCCG AATTTAACTG TTTTCAGGAA TGCTTAAAGC AATCAAAACA GAAGCCAAAA 
TTTACGTTCT ATGATGGGCC TCCTTTTGCA ACTGGACTGC CTCACTATGG ACATATACTT 
GCAGGGACAA TTAAAGATAT AGTTACCAGA TACGCTCACC AGAGTGGGTT TCATGTTGAC 
AGAAGATTTG GATGGGATTG TCATGGCTTA CCTGTGGAAT ATGAAATTGA TAAGACACTG 
GGAATCAGAG GACCAGAGGA TGTAGCCAAA ATGGGCATTG TAGAGTATAA CAATCAGTGC 
CGAGCAATTG TGATGAGATA TTCTACTGAG TGGAAGTCCA CTGTTAACAG ACTTGGCCGA 
TGGATTGACT TTGACAATGA CTACAAAACT CTGTATCCAC AATTCATGGA ATCTGTCTGG 
TGGGTCTTCA AGCAGCTCTA TGATAAAGGC CTTGTGTATA GAGGTGTGAA AGTCATGCCT 
TTCTCCACCG CGTGCAACAC TCCACTTTCC AACTTCGAAT CTCACCAGAA CTACAAGGAT 
GTTCAGGACC CTTCAATATT TGTAACTTTC CCTTTGGAGG AAGATGAAAA TGTGTCCTTG 
GTGGCTTGGA CGACCACTCC TTGGACTCTA CCTAGTAATC TTGCCCTGTG TGTTAATCCG 
GATATGCAGT ACGTGAAGAT TAAAGATGTT GTCAGAGGAA AATTACTCAT TTTAATGGAA 
GCCAGATTAC CAGCCCTCTA TAAGTCGGAG AGTGACTATG AGGTCCTTGA AAGATTTCCT 
GGTAGTCATC TCAAAGGCAA GAAGTACAGG CCCCTGTTTG ACTATTTTGT GCAGTGCAAG 
GAGAATGGTG CTTTCACGGT GCTCGCTGAC AGCTACGTGC GGGAGGAGGA GGGCACGGGA 
GTGGTGCACC AGGCTCCTTA CTTCGGTGCT GACGACTATC GGGTCTGTAT GGACTTGAGC 
ATCATTCAGA AAGACTCTCC TCCTGTGTGC CCTGTGGACG CTTCAGGCTG TTTCACGGCG 
GAGGTGACAG ATTTCACGGG ACAGTATGTG AAGGATGCTG ACAAAAACAT CATCAGGACC 
CTGAAGGAAA AAGGCCGACT TCTTGTCTCC AGCACCTTCA CTCACAGTTA CCCCTTCTGC 
TGGAGGTCAG ACACCCCCCT GATCTACAAA GCGGTGCCCA GCTGGTTCGT GCGGGTGGAG 
CACATGGTGG AGCAGCTCCT GAGGAACAAC GACTTGTGCT ACTGGGTCCC AGAGTTTGTG 
CGAGAAAAGC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGGCAGT TTCCAGAAAC 
AGATACTGGG GCACCCCCAT CCCCCTGTGG GTCAGTGATG ACTTCGAGGA GGTAGTATGC 
ATTGGATCGA TGGCTGAACT TGAAGAACTG TCGGGGGCAA AGGTCTCTGA TCTCCACAGA 
GAGAGCATTG ACCATTTGAC CATCCCTTCC CGCTGCGGGA AGGGAGTGTT GCATCGCATC 
TCTGAAGTGT TTGACTGTTG GTTCGAGAGC GGCAGCATGC CCTATGCTCA GGTTCATTAT 
CCGTTTGAAA ACAAGAGGGA GTTTGAGGAC ACATTTCCTG CAGACTTCAT TGCCGAAGGC 
ATTGACCAGA CCAGAGGATG GTTTTACACC CTGCTGGTGC TGGCCACCGC TCTCTTTGGA 
CAGCCGCCTT TCAGGAACGT CATTGTGAAC GGGCTCGTCC TGGCAAGTGA TGGTCAAAAA 
ATGAGCAAAC GAAAAAAGAA TTACCCGGAT CCACTTTCAA TCATCCATAA ATATGGTGCC 
GACGCCCTCA GATTATATCT GATCAACTCC CCTGTGGTGA AAGCAGAAAA CCTCCGATTT 
AAGGAGGAGG GTGTGCGTGA TGTCTTGAAG GACGTGCTGC TCCCTTGGTA CAATGCCTAC 
CGCTTCTTCA TCCAGAATGT CCTGAGGCTG CAGAAGGAGG AAGGAATGGA ATTTCTCTAC 
AATGAGAACA CAGTTAAAGG AAGTGCCAAC GTCACGGACC GGTGGGTCCT ATCCTGCATA 
CAGTCGCTGG TTGGGTTCTT TGAGACCGAA ATGGCAGCTT ACAGGCTCTA TACGGTGGTG 
CCTCGCCTGG TCAAGTTCGT GGATGTCCTA ACCAACTGGT ACGTCAGGAT GAACCGCAGA 
AGGCTGAAGG GTGAAAACGG GGTGGAGGAC TGTGTTACAG CCCTGGAGAC CTTGTTCAGT 
GTCCTGCTGT CTCTGTGCAG ACTGATGGCA CCCTATACAC CTTTTCTCAC CGAATTGATG 
TACCAGAATC TAAAGCTGCT GATCGACCCT GTTTCTGTCC AGGACAAGGA CACACACAGT 
ATCCACTACC TCATGCTGCC CCATGTTCGG GAGGAGCTGA TTGACAAGAA CACGGAGAGA 
GCAGTGTCTC GGATGCAGTC TGTGATCGAA CTTGGGCGAG TGATTCGAGA CCGCAAAACC 
ATTCCAATAA AGTATCCTTT GAAAGAAATT GTGGTTATCC ATCAGGATCC AGAAGCTCTT 
CATGAGATCA AGTCTTTGGA GAAGTATATT ATTGAGGAAT TAAACGTCCG AAAGGTCACG 
CTGTCTACAG ATAAAAACAA GTATGGCATT CGGCTAAGGG CAGAGCCTGA TCACATGGTC 
CTGGGGAAGC GTCTGAAGGG AGCCTTCAGG GCGGTGATGA CGTCCATCAA GAAGCTGAGC 
AGTGAAGAGC TGGAGCGCTT CCAGGAGAGC GGGACCATTG TTGTAGAAGG CCACGAGCTG 
CATGAAGAGG ACATCCGCCT CATGTACACC TTTGATCAGA CGACAGGAGG GACGACGCAG 
TATGAAGCAC ACTCTGACGC CCAGGTTTTG GTCCTCTTAG ATGTCACCCC TGACCAGTCA 
ATGGTGGATG AAGGAGTGGC TCGGGAGGTC ATCAACCGCA TCCAGAAACT TCGCAAAAAG 
TGCAATCTGG TTCCAACTGA TGAAATAACA GTTTATTATA AAGCGACGTC TGAAGGGAAG 
TATCTGAATA ATGTTATCGA AAGCCACACG GAGTTCATAT TTGCCACCAT AAAGGCTCCC 
TTGAAACCGT ATCCAGTTCC AGTGTCAGAT AAAGTCCTTA TTCAAGAAGA AACACAGTTA 
AAGGGCTCTG ACCTGGAAAT TACCATCACC AGAGGGTCGT CCCTGCCTGG TCCTGCTTGT 
GCGTATGTCA ACCTCAACAT TTGTACAGAT GGCAGTGAGC AAGGTGGAGT GTTGCTTCTG 
GAAAATCCAA AAGGAGATAA CAGGTTGGAC CTTTTAAAGC TGAAGAATGT TATTACTAGT 
ATCTTTGGGG TGAAAAATAC CGAGCTGGCT GTCCTTCACG GTGCCACAGA AATACGAAAC 
CAGACTGACT TACTGAGTCT TAGTGGAAAA ACACTTCGCG TGGCCGCGGG GTCGGCCCCT 
GCTGTGGTGG ATGGCCCCAG CCCTGCTCTC TGCCGCTATA TCAACCTGCA GCTCCTGAAC 
GCAGGCCCGC AAGAGTGTGT GAAGGGAACA GTGGGCACGC TCCTGCTGGA AAACCCACCC 
GGGCAGAACG GACTCAGCCA CCAGGGCCTT CTGTATGAAG CAGCCAAGGT GTTTGGCCTC 
CGCAGCAGGA AGCTCAAGCT GTTTCTGAAC GAGACTCAGA CACACGAAAT TACGGAAGAA 
ATCCCCGTGA AGACTTTGAA CATGAAGACT GTGTATGTCT CTGTATTACC GACGACGGCA 
GACTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011233649.1
CDS	38..3826
Translation	MVQQVPENINFPAEEEKILQFWSEFNCFQECLKQSKQKPKFTFYDGPPFATGLPHYGHILAGTIKDIVTRYAHQSGFHVDRRFGWDCHGLPVEYEIDKTLGIRGPEDVAKMGIVEYNNQCRAIVMRYSTEWKSTVNRLGRWIDFDNDYKTLYPQFMESVWWVFKQLYDKGLVYRGVKVMPFSTACNTPLSNFESHQNYKDVQDPSIFVTFPLEEDENVSLVAWTTTPWTLPSNLALCVNPDMQYVKIKDVVRGKLLILMEARLPALYKSESDYEVLERFPGSHLKGKKYRPLFDYFVQCKENGAFTVLADSYVREEEGTGVVHQAPYFGADDYRVCMDLSIIQKDSPPVCPVDASGCFTAEVTDFTGQYVKDADKNIIRTLKEKGRLLVSSTFTHSYPFCWRSDTPLIYKAVPSWFVRVEHMVEQLLRNNDLCYWVPEFVREKRFGNWLKDARDWAVSRNRYWGTPIPLWVSDDFEEVVCIGSMAELEELSGAKVSDLHRESIDHLTIPSRCGKGVLHRISEVFDCWFESGSMPYAQVHYPFENKREFEDTFPADFIAEGIDQTRGWFYTLLVLATALFGQPPFRNVIVNGLVLASDGQKMSKRKKNYPDPLSIIHKYGADALRLYLINSPVVKAENLRFKEEGVRDVLKDVLLPWYNAYRFFIQNVLRLQKEEGMEFLYNENTVKGSANVTDRWVLSCIQSLVGFFETEMAAYRLYTVVPRLVKFVDVLTNWYVRMNRRRLKGENGVEDCVTALETLFSVLLSLCRLMAPYTPFLTELMYQNLKLLIDPVSVQDKDTHSIHYLMLPHVREELIDKNTERAVSRMQSVIELGRVIRDRKTIPIKYPLKEIVVIHQDPEALHEIKSLEKYIIEELNVRKVTLSTDKNKYGIRLRAEPDHMVLGKRLKGAFRAVMTSIKKLSSEELERFQESGTIVVEGHELHEEDIRLMYTFDQTTGGTTQYEAHSDAQVLVLLDVTPDQSMVDEGVAREVINRIQKLRKKCNLVPTDEITVYYKATSEGKYLNNVIESHTEFIFATIKAPLKPYPVPVSDKVLIQEETQLKGSDLEITITRGSSLPGPACAYVNLNICTDGSEQGGVLLLENPKGDNRLDLLKLKNVITSIFGVKNTELAVLHGATEIRNQTDLLSLSGKTLRVAAGSAPAVVDGPSPALCRYINLQLLNAGPQECVKGTVGTLLLENPPGQNGLSHQGLLYEAAKVFGLRSRKLKLFLNETQTHEITEEIPVKTLNMKTVYVSVLPTTADF

Target ORF information:

RefSeq Version	XM_011235347.2
Organism	Ailuropoda melanoleuca(giant panda)
Definition	Ailuropoda melanoleuca isoleucyl-tRNA synthetase (IARS), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011235347.2

ORF Insert Sequence:

ATGGTTCAGC AAGTTCCAGA AAACATCAAT TTTCCTGCTG AAGAAGAGAA AATCTTACAG 
TTCTGGTCCG AATTTAACTG TTTTCAGGAA TGCTTAAAGC AATCAAAACA GAAGCCAAAA 
TTTACGTTCT ATGATGGGCC TCCTTTTGCA ACTGGACTGC CTCACTATGG ACATATACTT 
GCAGGGACAA TTAAAGATAT AGTTACCAGA TACGCTCACC AGAGTGGGTT TCATGTTGAC 
AGAAGATTTG GATGGGATTG TCATGGCTTA CCTGTGGAAT ATGAAATTGA TAAGACACTG 
GGAATCAGAG GACCAGAGGA TGTAGCCAAA ATGGGCATTG TAGAGTATAA CAATCAGTGC 
CGAGCAATTG TGATGAGATA TTCTACTGAG TGGAAGTCCA CTGTTAACAG ACTTGGCCGA 
TGGATTGACT TTGACAATGA CTACAAAACT CTGTATCCAC AATTCATGGA ATCTGTCTGG 
TGGGTCTTCA AGCAGCTCTA TGATAAAGGC CTTGTGTATA GAGGTGTGAA AGTCATGCCT 
TTCTCCACCG CGTGCAACAC TCCACTTTCC AACTTCGAAT CTCACCAGAA CTACAAGGAT 
GTTCAGGACC CTTCAATATT TGTAACTTTC CCTTTGGAGG AAGATGAAAA TGTGTCCTTG 
GTGGCTTGGA CGACCACTCC TTGGACTCTA CCTAGTAATC TTGCCCTGTG TGTTAATCCG 
GATATGCAGT ACGTGAAGAT TAAAGATGTT GTCAGAGGAA AATTACTCAT TTTAATGGAA 
GCCAGATTAC CAGCCCTCTA TAAGTCGGAG AGTGACTATG AGGTCCTTGA AAGATTTCCT 
GGTAGTCATC TCAAAGGCAA GAAGTACAGG CCCCTGTTTG ACTATTTTGT GCAGTGCAAG 
GAGAATGGTG CTTTCACGGT GCTCGCTGAC AGCTACGTGC GGGAGGAGGA GGGCACGGGA 
GTGGTGCACC AGGCTCCTTA CTTCGGTGCT GACGACTATC GGGTCTGTAT GGACTTGAGC 
ATCATTCAGA AAGACTCTCC TCCTGTGTGC CCTGTGGACG CTTCAGGCTG TTTCACGGCG 
GAGGTGACAG ATTTCACGGG ACAGTATGTG AAGGATGCTG ACAAAAACAT CATCAGGACC 
CTGAAGGAAA AAGGCCGACT TCTTGTCTCC AGCACCTTCA CTCACAGTTA CCCCTTCTGC 
TGGAGGTCAG ACACCCCCCT GATCTACAAA GCGGTGCCCA GCTGGTTCGT GCGGGTGGAG 
CACATGGTGG AGCAGCTCCT GAGGAACAAC GACTTGTGCT ACTGGGTCCC AGAGTTTGTG 
CGAGAAAAGC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGGCAGT TTCCAGAAAC 
AGATACTGGG GCACCCCCAT CCCCCTGTGG GTCAGTGATG ACTTCGAGGA GGTAGTATGC 
ATTGGATCGA TGGCTGAACT TGAAGAACTG TCGGGGGCAA AGGTCTCTGA TCTCCACAGA 
GAGAGCATTG ACCATTTGAC CATCCCTTCC CGCTGCGGGA AGGGAGTGTT GCATCGCATC 
TCTGAAGTGT TTGACTGTTG GTTCGAGAGC GGCAGCATGC CCTATGCTCA GGTTCATTAT 
CCGTTTGAAA ACAAGAGGGA GTTTGAGGAC ACATTTCCTG CAGACTTCAT TGCCGAAGGC 
ATTGACCAGA CCAGAGGATG GTTTTACACC CTGCTGGTGC TGGCCACCGC TCTCTTTGGA 
CAGCCGCCTT TCAGGAACGT CATTGTGAAC GGGCTCGTCC TGGCAAGTGA TGGTCAAAAA 
ATGAGCAAAC GAAAAAAGAA TTACCCGGAT CCACTTTCAA TCATCCATAA ATATGGTGCC 
GACGCCCTCA GATTATATCT GATCAACTCC CCTGTGGTGA AAGCAGAAAA CCTCCGATTT 
AAGGAGGAGG GTGTGCGTGA TGTCTTGAAG GACGTGCTGC TCCCTTGGTA CAATGCCTAC 
CGCTTCTTCA TCCAGAATGT CCTGAGGCTG CAGAAGGAGG AAGGAATGGA ATTTCTCTAC 
AATGAGAACA CAGTTAAAGG AAGTGCCAAC GTCACGGACC GGTGGGTCCT ATCCTGCATA 
CAGTCGCTGG TTGGGTTCTT TGAGACCGAA ATGGCAGCTT ACAGGCTCTA TACGGTGGTG 
CCTCGCCTGG TCAAGTTCGT GGATGTCCTA ACCAACTGGT ACGTCAGGAT GAACCGCAGA 
AGGCTGAAGG GTGAAAACGG GGTGGAGGAC TGTGTTACAG CCCTGGAGAC CTTGTTCAGT 
GTCCTGCTGT CTCTGTGCAG ACTGATGGCA CCCTATACAC CTTTTCTCAC CGAATTGATG 
TACCAGAATC TAAAGCTGCT GATCGACCCT GTTTCTGTCC AGGACAAGGA CACACACAGT 
ATCCACTACC TCATGCTGCC CCATGTTCGG GAGGAGCTGA TTGACAAGAA CACGGAGAGA 
GCAGTGTCTC GGATGCAGTC TGTGATCGAA CTTGGGCGAG TGATTCGAGA CCGCAAAACC 
ATTCCAATAA AGTATCCTTT GAAAGAAATT GTGGTTATCC ATCAGGATCC AGAAGCTCTT 
CATGAGATCA AGTCTTTGGA GAAGTATATT ATTGAGGAAT TAAACGTCCG AAAGGTCACG 
CTGTCTACAG ATAAAAACAA GTATGGCATT CGGCTAAGGG CAGAGCCTGA TCACATGGTC 
CTGGGGAAGC GTCTGAAGGG AGCCTTCAGG GCGGTGATGA CGTCCATCAA GAAGCTGAGC 
AGTGAAGAGC TGGAGCGCTT CCAGGAGAGC GGGACCATTG TTGTAGAAGG CCACGAGCTG 
CATGAAGAGG ACATCCGCCT CATGTACACC TTTGATCAGA CGACAGGAGG GACGACGCAG 
TATGAAGCAC ACTCTGACGC CCAGGTTTTG GTCCTCTTAG ATGTCACCCC TGACCAGTCA 
ATGGTGGATG AAGGAGTGGC TCGGGAGGTC ATCAACCGCA TCCAGAAACT TCGCAAAAAG 
TGCAATCTGG TTCCAACTGA TGAAATAACA GTTTATTATA AAGCGACGTC TGAAGGGAAG 
TATCTGAATA ATGTTATCGA AAGCCACACG GAGTTCATAT TTGCCACCAT AAAGGCTCCC 
TTGAAACCGT ATCCAGTTCC AGTGTCAGAT AAAGTCCTTA TTCAAGAAGA AACACAGTTA 
AAGGGCTCTG ACCTGGAAAT TACCATCACC AGAGGGTCGT CCCTGCCTGG TCCTGCTTGT 
GCGTATGTCA ACCTCAACAT TTGTACAGAT GGCAGTGAGC AAGGTGGAGT GTTGCTTCTG 
GAAAATCCAA AAGGAGATAA CAGGTTGGAC CTTTTAAAGC TGAAGAATGT TATTACTAGT 
ATCTTTGGGG TGAAAAATAC CGAGCTGGCT GTCCTTCACG GTGCCACAGA AATACGAAAC 
CAGACTGACT TACTGAGTCT TAGTGGAAAA ACACTTCGCG TGGCCGCGGG GTCGGCCCCT 
GCTGTGGTGG ATGGCCCCAG CCCTGCTCTC TGCCGCTATA TCAACCTGCA GCTCCTGAAC 
GCAGGCCCGC AAGAGTGTGT GAAGGGAACA GTGGGCACGC TCCTGCTGGA AAACCCACCC 
GGGCAGAACG GACTCAGCCA CCAGGGCCTT CTGTATGAAG CAGCCAAGGT GTTTGGCCTC 
CGCAGCAGGA AGCTCAAGCT GTTTCTGAAC GAGACTCAGA CACACGAAAT TACGGAAGAA 
ATCCCCGTGA AGACTTTGAA CATGAAGACT GTGTATGTCT CTGTATTACC GACGACGGCA 
GACTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IARS ( XM_019808397.1 ) cDNA ORF clone, Ailuropoda melanoleuca(giant panda) -> XP_019663956.1 Ailuropoda melanoleuca isoleucyl-tRNA synthetase (IARS), transcript variant X2, mRNA.

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CloneID	OAc14733
Clone ID Related Accession (Same CDS sequence)	XM_011235347.2 , XM_019808397.1
Accession Version	XM_019808397.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-27
Organism	Ailuropoda melanoleuca(giant panda)
Product	isoleucine--tRNA ligase, cytoplasmic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003218612.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ailuropoda melanoleuca Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTCAGC AAGTTCCAGA AAACATCAAT TTTCCTGCTG AAGAAGAGAA AATCTTACAG 
TTCTGGTCCG AATTTAACTG TTTTCAGGAA TGCTTAAAGC AATCAAAACA GAAGCCAAAA 
TTTACGTTCT ATGATGGGCC TCCTTTTGCA ACTGGACTGC CTCACTATGG ACATATACTT 
GCAGGGACAA TTAAAGATAT AGTTACCAGA TACGCTCACC AGAGTGGGTT TCATGTTGAC 
AGAAGATTTG GATGGGATTG TCATGGCTTA CCTGTGGAAT ATGAAATTGA TAAGACACTG 
GGAATCAGAG GACCAGAGGA TGTAGCCAAA ATGGGCATTG TAGAGTATAA CAATCAGTGC 
CGAGCAATTG TGATGAGATA TTCTACTGAG TGGAAGTCCA CTGTTAACAG ACTTGGCCGA 
TGGATTGACT TTGACAATGA CTACAAAACT CTGTATCCAC AATTCATGGA ATCTGTCTGG 
TGGGTCTTCA AGCAGCTCTA TGATAAAGGC CTTGTGTATA GAGGTGTGAA AGTCATGCCT 
TTCTCCACCG CGTGCAACAC TCCACTTTCC AACTTCGAAT CTCACCAGAA CTACAAGGAT 
GTTCAGGACC CTTCAATATT TGTAACTTTC CCTTTGGAGG AAGATGAAAA TGTGTCCTTG 
GTGGCTTGGA CGACCACTCC TTGGACTCTA CCTAGTAATC TTGCCCTGTG TGTTAATCCG 
GATATGCAGT ACGTGAAGAT TAAAGATGTT GTCAGAGGAA AATTACTCAT TTTAATGGAA 
GCCAGATTAC CAGCCCTCTA TAAGTCGGAG AGTGACTATG AGGTCCTTGA AAGATTTCCT 
GGTAGTCATC TCAAAGGCAA GAAGTACAGG CCCCTGTTTG ACTATTTTGT GCAGTGCAAG 
GAGAATGGTG CTTTCACGGT GCTCGCTGAC AGCTACGTGC GGGAGGAGGA GGGCACGGGA 
GTGGTGCACC AGGCTCCTTA CTTCGGTGCT GACGACTATC GGGTCTGTAT GGACTTGAGC 
ATCATTCAGA AAGACTCTCC TCCTGTGTGC CCTGTGGACG CTTCAGGCTG TTTCACGGCG 
GAGGTGACAG ATTTCACGGG ACAGTATGTG AAGGATGCTG ACAAAAACAT CATCAGGACC 
CTGAAGGAAA AAGGCCGACT TCTTGTCTCC AGCACCTTCA CTCACAGTTA CCCCTTCTGC 
TGGAGGTCAG ACACCCCCCT GATCTACAAA GCGGTGCCCA GCTGGTTCGT GCGGGTGGAG 
CACATGGTGG AGCAGCTCCT GAGGAACAAC GACTTGTGCT ACTGGGTCCC AGAGTTTGTG 
CGAGAAAAGC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGGCAGT TTCCAGAAAC 
AGATACTGGG GCACCCCCAT CCCCCTGTGG GTCAGTGATG ACTTCGAGGA GGTAGTATGC 
ATTGGATCGA TGGCTGAACT TGAAGAACTG TCGGGGGCAA AGGTCTCTGA TCTCCACAGA 
GAGAGCATTG ACCATTTGAC CATCCCTTCC CGCTGCGGGA AGGGAGTGTT GCATCGCATC 
TCTGAAGTGT TTGACTGTTG GTTCGAGAGC GGCAGCATGC CCTATGCTCA GGTTCATTAT 
CCGTTTGAAA ACAAGAGGGA GTTTGAGGAC ACATTTCCTG CAGACTTCAT TGCCGAAGGC 
ATTGACCAGA CCAGAGGATG GTTTTACACC CTGCTGGTGC TGGCCACCGC TCTCTTTGGA 
CAGCCGCCTT TCAGGAACGT CATTGTGAAC GGGCTCGTCC TGGCAAGTGA TGGTCAAAAA 
ATGAGCAAAC GAAAAAAGAA TTACCCGGAT CCACTTTCAA TCATCCATAA ATATGGTGCC 
GACGCCCTCA GATTATATCT GATCAACTCC CCTGTGGTGA AAGCAGAAAA CCTCCGATTT 
AAGGAGGAGG GTGTGCGTGA TGTCTTGAAG GACGTGCTGC TCCCTTGGTA CAATGCCTAC 
CGCTTCTTCA TCCAGAATGT CCTGAGGCTG CAGAAGGAGG AAGGAATGGA ATTTCTCTAC 
AATGAGAACA CAGTTAAAGG AAGTGCCAAC GTCACGGACC GGTGGGTCCT ATCCTGCATA 
CAGTCGCTGG TTGGGTTCTT TGAGACCGAA ATGGCAGCTT ACAGGCTCTA TACGGTGGTG 
CCTCGCCTGG TCAAGTTCGT GGATGTCCTA ACCAACTGGT ACGTCAGGAT GAACCGCAGA 
AGGCTGAAGG GTGAAAACGG GGTGGAGGAC TGTGTTACAG CCCTGGAGAC CTTGTTCAGT 
GTCCTGCTGT CTCTGTGCAG ACTGATGGCA CCCTATACAC CTTTTCTCAC CGAATTGATG 
TACCAGAATC TAAAGCTGCT GATCGACCCT GTTTCTGTCC AGGACAAGGA CACACACAGT 
ATCCACTACC TCATGCTGCC CCATGTTCGG GAGGAGCTGA TTGACAAGAA CACGGAGAGA 
GCAGTGTCTC GGATGCAGTC TGTGATCGAA CTTGGGCGAG TGATTCGAGA CCGCAAAACC 
ATTCCAATAA AGTATCCTTT GAAAGAAATT GTGGTTATCC ATCAGGATCC AGAAGCTCTT 
CATGAGATCA AGTCTTTGGA GAAGTATATT ATTGAGGAAT TAAACGTCCG AAAGGTCACG 
CTGTCTACAG ATAAAAACAA GTATGGCATT CGGCTAAGGG CAGAGCCTGA TCACATGGTC 
CTGGGGAAGC GTCTGAAGGG AGCCTTCAGG GCGGTGATGA CGTCCATCAA GAAGCTGAGC 
AGTGAAGAGC TGGAGCGCTT CCAGGAGAGC GGGACCATTG TTGTAGAAGG CCACGAGCTG 
CATGAAGAGG ACATCCGCCT CATGTACACC TTTGATCAGA CGACAGGAGG GACGACGCAG 
TATGAAGCAC ACTCTGACGC CCAGGTTTTG GTCCTCTTAG ATGTCACCCC TGACCAGTCA 
ATGGTGGATG AAGGAGTGGC TCGGGAGGTC ATCAACCGCA TCCAGAAACT TCGCAAAAAG 
TGCAATCTGG TTCCAACTGA TGAAATAACA GTTTATTATA AAGCGACGTC TGAAGGGAAG 
TATCTGAATA ATGTTATCGA AAGCCACACG GAGTTCATAT TTGCCACCAT AAAGGCTCCC 
TTGAAACCGT ATCCAGTTCC AGTGTCAGAT AAAGTCCTTA TTCAAGAAGA AACACAGTTA 
AAGGGCTCTG ACCTGGAAAT TACCATCACC AGAGGGTCGT CCCTGCCTGG TCCTGCTTGT 
GCGTATGTCA ACCTCAACAT TTGTACAGAT GGCAGTGAGC AAGGTGGAGT GTTGCTTCTG 
GAAAATCCAA AAGGAGATAA CAGGTTGGAC CTTTTAAAGC TGAAGAATGT TATTACTAGT 
ATCTTTGGGG TGAAAAATAC CGAGCTGGCT GTCCTTCACG GTGCCACAGA AATACGAAAC 
CAGACTGACT TACTGAGTCT TAGTGGAAAA ACACTTCGCG TGGCCGCGGG GTCGGCCCCT 
GCTGTGGTGG ATGGCCCCAG CCCTGCTCTC TGCCGCTATA TCAACCTGCA GCTCCTGAAC 
GCAGGCCCGC AAGAGTGTGT GAAGGGAACA GTGGGCACGC TCCTGCTGGA AAACCCACCC 
GGGCAGAACG GACTCAGCCA CCAGGGCCTT CTGTATGAAG CAGCCAAGGT GTTTGGCCTC 
CGCAGCAGGA AGCTCAAGCT GTTTCTGAAC GAGACTCAGA CACACGAAAT TACGGAAGAA 
ATCCCCGTGA AGACTTTGAA CATGAAGACT GTGTATGTCT CTGTATTACC GACGACGGCA 
GACTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019663956.1
CDS	71..3859
Translation	MVQQVPENINFPAEEEKILQFWSEFNCFQECLKQSKQKPKFTFYDGPPFATGLPHYGHILAGTIKDIVTRYAHQSGFHVDRRFGWDCHGLPVEYEIDKTLGIRGPEDVAKMGIVEYNNQCRAIVMRYSTEWKSTVNRLGRWIDFDNDYKTLYPQFMESVWWVFKQLYDKGLVYRGVKVMPFSTACNTPLSNFESHQNYKDVQDPSIFVTFPLEEDENVSLVAWTTTPWTLPSNLALCVNPDMQYVKIKDVVRGKLLILMEARLPALYKSESDYEVLERFPGSHLKGKKYRPLFDYFVQCKENGAFTVLADSYVREEEGTGVVHQAPYFGADDYRVCMDLSIIQKDSPPVCPVDASGCFTAEVTDFTGQYVKDADKNIIRTLKEKGRLLVSSTFTHSYPFCWRSDTPLIYKAVPSWFVRVEHMVEQLLRNNDLCYWVPEFVREKRFGNWLKDARDWAVSRNRYWGTPIPLWVSDDFEEVVCIGSMAELEELSGAKVSDLHRESIDHLTIPSRCGKGVLHRISEVFDCWFESGSMPYAQVHYPFENKREFEDTFPADFIAEGIDQTRGWFYTLLVLATALFGQPPFRNVIVNGLVLASDGQKMSKRKKNYPDPLSIIHKYGADALRLYLINSPVVKAENLRFKEEGVRDVLKDVLLPWYNAYRFFIQNVLRLQKEEGMEFLYNENTVKGSANVTDRWVLSCIQSLVGFFETEMAAYRLYTVVPRLVKFVDVLTNWYVRMNRRRLKGENGVEDCVTALETLFSVLLSLCRLMAPYTPFLTELMYQNLKLLIDPVSVQDKDTHSIHYLMLPHVREELIDKNTERAVSRMQSVIELGRVIRDRKTIPIKYPLKEIVVIHQDPEALHEIKSLEKYIIEELNVRKVTLSTDKNKYGIRLRAEPDHMVLGKRLKGAFRAVMTSIKKLSSEELERFQESGTIVVEGHELHEEDIRLMYTFDQTTGGTTQYEAHSDAQVLVLLDVTPDQSMVDEGVAREVINRIQKLRKKCNLVPTDEITVYYKATSEGKYLNNVIESHTEFIFATIKAPLKPYPVPVSDKVLIQEETQLKGSDLEITITRGSSLPGPACAYVNLNICTDGSEQGGVLLLENPKGDNRLDLLKLKNVITSIFGVKNTELAVLHGATEIRNQTDLLSLSGKTLRVAAGSAPAVVDGPSPALCRYINLQLLNAGPQECVKGTVGTLLLENPPGQNGLSHQGLLYEAAKVFGLRSRKLKLFLNETQTHEITEEIPVKTLNMKTVYVSVLPTTADF

Target ORF information:

RefSeq Version	XM_019808397.1
Organism	Ailuropoda melanoleuca(giant panda)
Definition	Ailuropoda melanoleuca isoleucyl-tRNA synthetase (IARS), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019808397.1

ORF Insert Sequence:

ATGGTTCAGC AAGTTCCAGA AAACATCAAT TTTCCTGCTG AAGAAGAGAA AATCTTACAG 
TTCTGGTCCG AATTTAACTG TTTTCAGGAA TGCTTAAAGC AATCAAAACA GAAGCCAAAA 
TTTACGTTCT ATGATGGGCC TCCTTTTGCA ACTGGACTGC CTCACTATGG ACATATACTT 
GCAGGGACAA TTAAAGATAT AGTTACCAGA TACGCTCACC AGAGTGGGTT TCATGTTGAC 
AGAAGATTTG GATGGGATTG TCATGGCTTA CCTGTGGAAT ATGAAATTGA TAAGACACTG 
GGAATCAGAG GACCAGAGGA TGTAGCCAAA ATGGGCATTG TAGAGTATAA CAATCAGTGC 
CGAGCAATTG TGATGAGATA TTCTACTGAG TGGAAGTCCA CTGTTAACAG ACTTGGCCGA 
TGGATTGACT TTGACAATGA CTACAAAACT CTGTATCCAC AATTCATGGA ATCTGTCTGG 
TGGGTCTTCA AGCAGCTCTA TGATAAAGGC CTTGTGTATA GAGGTGTGAA AGTCATGCCT 
TTCTCCACCG CGTGCAACAC TCCACTTTCC AACTTCGAAT CTCACCAGAA CTACAAGGAT 
GTTCAGGACC CTTCAATATT TGTAACTTTC CCTTTGGAGG AAGATGAAAA TGTGTCCTTG 
GTGGCTTGGA CGACCACTCC TTGGACTCTA CCTAGTAATC TTGCCCTGTG TGTTAATCCG 
GATATGCAGT ACGTGAAGAT TAAAGATGTT GTCAGAGGAA AATTACTCAT TTTAATGGAA 
GCCAGATTAC CAGCCCTCTA TAAGTCGGAG AGTGACTATG AGGTCCTTGA AAGATTTCCT 
GGTAGTCATC TCAAAGGCAA GAAGTACAGG CCCCTGTTTG ACTATTTTGT GCAGTGCAAG 
GAGAATGGTG CTTTCACGGT GCTCGCTGAC AGCTACGTGC GGGAGGAGGA GGGCACGGGA 
GTGGTGCACC AGGCTCCTTA CTTCGGTGCT GACGACTATC GGGTCTGTAT GGACTTGAGC 
ATCATTCAGA AAGACTCTCC TCCTGTGTGC CCTGTGGACG CTTCAGGCTG TTTCACGGCG 
GAGGTGACAG ATTTCACGGG ACAGTATGTG AAGGATGCTG ACAAAAACAT CATCAGGACC 
CTGAAGGAAA AAGGCCGACT TCTTGTCTCC AGCACCTTCA CTCACAGTTA CCCCTTCTGC 
TGGAGGTCAG ACACCCCCCT GATCTACAAA GCGGTGCCCA GCTGGTTCGT GCGGGTGGAG 
CACATGGTGG AGCAGCTCCT GAGGAACAAC GACTTGTGCT ACTGGGTCCC AGAGTTTGTG 
CGAGAAAAGC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGGCAGT TTCCAGAAAC 
AGATACTGGG GCACCCCCAT CCCCCTGTGG GTCAGTGATG ACTTCGAGGA GGTAGTATGC 
ATTGGATCGA TGGCTGAACT TGAAGAACTG TCGGGGGCAA AGGTCTCTGA TCTCCACAGA 
GAGAGCATTG ACCATTTGAC CATCCCTTCC CGCTGCGGGA AGGGAGTGTT GCATCGCATC 
TCTGAAGTGT TTGACTGTTG GTTCGAGAGC GGCAGCATGC CCTATGCTCA GGTTCATTAT 
CCGTTTGAAA ACAAGAGGGA GTTTGAGGAC ACATTTCCTG CAGACTTCAT TGCCGAAGGC 
ATTGACCAGA CCAGAGGATG GTTTTACACC CTGCTGGTGC TGGCCACCGC TCTCTTTGGA 
CAGCCGCCTT TCAGGAACGT CATTGTGAAC GGGCTCGTCC TGGCAAGTGA TGGTCAAAAA 
ATGAGCAAAC GAAAAAAGAA TTACCCGGAT CCACTTTCAA TCATCCATAA ATATGGTGCC 
GACGCCCTCA GATTATATCT GATCAACTCC CCTGTGGTGA AAGCAGAAAA CCTCCGATTT 
AAGGAGGAGG GTGTGCGTGA TGTCTTGAAG GACGTGCTGC TCCCTTGGTA CAATGCCTAC 
CGCTTCTTCA TCCAGAATGT CCTGAGGCTG CAGAAGGAGG AAGGAATGGA ATTTCTCTAC 
AATGAGAACA CAGTTAAAGG AAGTGCCAAC GTCACGGACC GGTGGGTCCT ATCCTGCATA 
CAGTCGCTGG TTGGGTTCTT TGAGACCGAA ATGGCAGCTT ACAGGCTCTA TACGGTGGTG 
CCTCGCCTGG TCAAGTTCGT GGATGTCCTA ACCAACTGGT ACGTCAGGAT GAACCGCAGA 
AGGCTGAAGG GTGAAAACGG GGTGGAGGAC TGTGTTACAG CCCTGGAGAC CTTGTTCAGT 
GTCCTGCTGT CTCTGTGCAG ACTGATGGCA CCCTATACAC CTTTTCTCAC CGAATTGATG 
TACCAGAATC TAAAGCTGCT GATCGACCCT GTTTCTGTCC AGGACAAGGA CACACACAGT 
ATCCACTACC TCATGCTGCC CCATGTTCGG GAGGAGCTGA TTGACAAGAA CACGGAGAGA 
GCAGTGTCTC GGATGCAGTC TGTGATCGAA CTTGGGCGAG TGATTCGAGA CCGCAAAACC 
ATTCCAATAA AGTATCCTTT GAAAGAAATT GTGGTTATCC ATCAGGATCC AGAAGCTCTT 
CATGAGATCA AGTCTTTGGA GAAGTATATT ATTGAGGAAT TAAACGTCCG AAAGGTCACG 
CTGTCTACAG ATAAAAACAA GTATGGCATT CGGCTAAGGG CAGAGCCTGA TCACATGGTC 
CTGGGGAAGC GTCTGAAGGG AGCCTTCAGG GCGGTGATGA CGTCCATCAA GAAGCTGAGC 
AGTGAAGAGC TGGAGCGCTT CCAGGAGAGC GGGACCATTG TTGTAGAAGG CCACGAGCTG 
CATGAAGAGG ACATCCGCCT CATGTACACC TTTGATCAGA CGACAGGAGG GACGACGCAG 
TATGAAGCAC ACTCTGACGC CCAGGTTTTG GTCCTCTTAG ATGTCACCCC TGACCAGTCA 
ATGGTGGATG AAGGAGTGGC TCGGGAGGTC ATCAACCGCA TCCAGAAACT TCGCAAAAAG 
TGCAATCTGG TTCCAACTGA TGAAATAACA GTTTATTATA AAGCGACGTC TGAAGGGAAG 
TATCTGAATA ATGTTATCGA AAGCCACACG GAGTTCATAT TTGCCACCAT AAAGGCTCCC 
TTGAAACCGT ATCCAGTTCC AGTGTCAGAT AAAGTCCTTA TTCAAGAAGA AACACAGTTA 
AAGGGCTCTG ACCTGGAAAT TACCATCACC AGAGGGTCGT CCCTGCCTGG TCCTGCTTGT 
GCGTATGTCA ACCTCAACAT TTGTACAGAT GGCAGTGAGC AAGGTGGAGT GTTGCTTCTG 
GAAAATCCAA AAGGAGATAA CAGGTTGGAC CTTTTAAAGC TGAAGAATGT TATTACTAGT 
ATCTTTGGGG TGAAAAATAC CGAGCTGGCT GTCCTTCACG GTGCCACAGA AATACGAAAC 
CAGACTGACT TACTGAGTCT TAGTGGAAAA ACACTTCGCG TGGCCGCGGG GTCGGCCCCT 
GCTGTGGTGG ATGGCCCCAG CCCTGCTCTC TGCCGCTATA TCAACCTGCA GCTCCTGAAC 
GCAGGCCCGC AAGAGTGTGT GAAGGGAACA GTGGGCACGC TCCTGCTGGA AAACCCACCC 
GGGCAGAACG GACTCAGCCA CCAGGGCCTT CTGTATGAAG CAGCCAAGGT GTTTGGCCTC 
CGCAGCAGGA AGCTCAAGCT GTTTCTGAAC GAGACTCAGA CACACGAAAT TACGGAAGAA 
ATCCCCGTGA AGACTTTGAA CATGAAGACT GTGTATGTCT CTGTATTACC GACGACGGCA 
GACTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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IARS cDNA ORF clone, Ailuropoda melanoleuca(giant panda)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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