LOC100477320 cDNA ORF clone, Ailuropoda melanoleuca(giant panda)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100477320
Entrez Gene ID	100477320
Full Name	E3 ubiquitin/ISG15 ligase TRIM25-like
Gene Type	protein-coding
Organism	Ailuropoda melanoleuca(giant panda)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019800285.1	XP_019655844.1	LOW QUALITY PROTEIN: E3 ubiquitin/ISG15 ligase TRIM25-like

Pathways from BioSystems
Gene Ontology

The following LOC100477320 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100477320 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAc93232	XM_019800285.1 Latest version!	Ailuropoda melanoleuca E3 ubiquitin/ISG15 ligase TRIM25-like (LOC100477320), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100477320 ( XM_019800285.1 ) cDNA ORF clone, Ailuropoda melanoleuca(giant panda) -> XP_019655844.1 Ailuropoda melanoleuca E3 ubiquitin/ISG15 ligase TRIM25-like (LOC100477320), mRNA.

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CloneID	OAc93232
Clone ID Related Accession (Same CDS sequence)	XM_019800285.1
Accession Version	XM_019800285.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2157bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-27
Organism	Ailuropoda melanoleuca(giant panda)
Product	LOW QUALITY PROTEIN: E3 ubiquitin/ISG15 ligase TRIM25-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003217653.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ailuropoda melanoleuca Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 10% of CDS bases frameshifts :: corrected 2 indels ##RefSeq-Attributes-END##

ATGTCAGGGA ACAAAGCTGG GGGCAATCTT CGAGGAGGGG GGGCCAACTT GATAGGGAGA 
TCACAAAACA GCAGCCAAAG AGAAAGGGCA GCCAAGCCTC TAGCCCTCCC TGACATGCAG 
AAAGTTTCAC GGCCAGTCCA GGACCACAAC CCAGCCCTGC AGCCCCCTGC CCAGCCCTCA 
AGACCTCCCC AAACTCAGCT CCTGATGAGT AACAGCAGTC TCCGCACCTT CGAGGACCAA 
GTCCTCTGTC CCATTTGCCT GGAGGTGTTC CGCAATCCGG TCACCACCGC CTGCGGGCAC 
AACTTCTGCA TGACCTGCCT CCAAGGTTTC TGGGACCACC AGGCTACCGT GGGCGAGACG 
CTCTACTGCC CCCAGTGCCG AGAGAGCTTC CCCTCCAGAC CGCACCTCTG CAAGAATGTC 
ATCCTGGAGG AGATGGTGAC CTGCTTCAGC CAGGCCAAGG GCCAGACCTT GGGATCCTCC 
CGGGTCCTGG CCGGGCCCAG GGACGTGCCC TGCGACTTCT GTTCTCCGCA GAAGCTCAAG 
TCAGTCAAGT CCTGTCTGCA GTGCATGGCC TCCCTGTGCG AGGAGCACCT GCGCAGCCAC 
TTCGAGGACC AGGCGTTCCA GGACCACCAG CTGCTGGAGC CCGTGTGGGA TCTCAAGAGC 
CGCCTGTGCC GCAAGCACCG CAAGCTGCGG CGGCTGTACT GCCGCACGGA AGGCTGCTGC 
GTGTGCGGGA CATGTCTGCT GGAGGAGCAC AAGAACCACG ACACCATCCC CCTGGAGGAG 
GAGCGCGCCC ACAAGGAGGT GGAGGTTCGG AAGGTCCAGG CCAATGTGGA AAACCAGATG 
CTGATCATCA ACTCTGACAG CCAGAAGCAC CGGGGGCAGG TGGCCTTTCT CTCGAAACTG 
ATCCAGACAA CACGTGAAGA GGTGAACACC TGCTTCTCAG AGATCATCCA GGAGGTCAAA 
CAGCTGCAGA TGAAGGTCTT GGATTTTGTG GAGAAAGAGG AGGCAGCGGC TCTGGGGAAG 
CTGGGCAGCT CCATCCAGCA GAGCCACAAC CGGCTTCTGA AGCTGGAGGG GGACAGCATC 
TGGCTCCGCA CCCTGCTCGC CAACAGGAGT GACCAGCAGT TTCTGCAGGA GTTCCCCAGG 
CTGAAGCACT TCCCCGCCTG CATGGAACCC CTGATGGGAA CCAACTGCGA GGAGAAGCAG 
GCCTTCCTCC AGTTGCCAGA GACCCTGGCA GAGCTCCGCA CCCGGCTGAT GGATGTGGGT 
CTCAGCTTCA TCAATCAGCT CCTTCTGAAG GGCATTAAGA TGAACTCCTA TGAGGTGCTG 
CCCCCCGCTG TGGACAGGAA AACACTTCTC AAGTGTTACT GCAACCTGAA CTTTGACCCC 
ACCACGGCCA GCGAGGAGCT GTTCCTGTTC AAGGAGACCC ACTCGGTGCT GAACCTGGGC 
ATCCTCCTGG AGCCCTTCGC CACCGGCGGC CCCTTCCCCG GCTTCAAGCA GTGGCCGCAG 
GTGCTTTGCT CGCGCGGCCT GTCCGAGGGC CGCCACTACT GGGAGGCCGA CGTGTCCAAC 
TCGTGGGTGT GCCTGGGCCT CCCTANNCAC ACCTACCGCC GCAGCCCCCC GTTCGGCGGC 
CGCCCGCGCC GCAACATCGT CTACCTGCTG GGCCGCAACC CCTATTCGTG GTGCCTGGAG 
TGGGACTCGC TCAAGTTCTC CGTGTGGCAC AACAACACGC AGACGGTGCT GCACGGCGGC 
TACCACCGCA CGCTCGGCGT GGCGCTCGAC TGNNNNNNNC CCGCCCCCGC CTGCCTCTCC 
TTCTACGGCG TGGCGGGCGG CGTGAGCCTG CTCTACCGCT TCCTCGTCTC CTTCCTGGAG 
CCGCTCTACC CCGCAGTCAT GGTCAGCAGC GGCGCCTCGG TCACGCTCAA GCAGCGCCCG 
GAGGCGGAGG CGGCGGCGAG GCAGACCCCT CGGTTCCCGC AGCCGCAGGA GAAGGGCTCT 
GAGCCGGCCT GGAGTTCCCC CCGGGACTGG GGACAGTCCG GTGGCCTCCG GAGGGCGGAT 
TCCTGGCCCG GGTGCACAGT CTCCCCCACC GTGGCGACCA CCCTCCTAAG CCCCACCAGA 
GGCCCCTGGC CTTGGTTGTC CAGGCTGGTG CCGGCTACAC AGCAACGTGT GGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019655844.1
CDS	21..2177
Translation	MSGNKAGGNLRGGGANLIGRSQNSSQRERAAKPLALPDMQKVSRPVQDHNPALQPPAQPSRPPQTQLLMSNSSLRTFEDQVLCPICLEVFRNPVTTACGHNFCMTCLQGFWDHQATVGETLYCPQCRESFPSRPHLCKNVILEEMVTCFSQAKGQTLGSSRVLAGPRDVPCDFCSPQKLKSVKSCLQCMASLCEEHLRSHFEDQAFQDHQLLEPVWDLKSRLCRKHRKLRRLYCRTEGCCVCGTCLLEEHKNHDTIPLEEERAHKEVEVRKVQANVENQMLIINSDSQKHRGQVAFLSKLIQTTREEVNTCFSEIIQEVKQLQMKVLDFVEKEEAAALGKLGSSIQQSHNRLLKLEGDSIWLRTLLANRSDQQFLQEFPRLKHFPACMEPLMGTNCEEKQAFLQLPETLAELRTRLMDVGLSFINQLLLKGIKMNSYEVLPPAVDRKTLLKCYCNLNFDPTTASEELFLFKETHSVLNLGILLEPFATGGPFPGFKQWPQVLCSRGLSEGRHYWEADVSNSWVCLGLPnullHTYRRSPPFGGRPRRNIVYLLGRNPYSWCLEWDSLKFSVWHNNTQTVLHGGYHRTLGVALDnullnullnullPAPACLSFYGVAGGVSLLYRFLVSFLEPLYPAVMVSSGASVTLKQRPEAEAAARQTPRFPQPQEKGSEPAWSSPRDWGQSGGLRRADSWPGCTVSPTVATTLLSPTRGPWPWLSRLVPATQQRVA

Target ORF information:

RefSeq Version	XM_019800285.1
Organism	Ailuropoda melanoleuca(giant panda)
Definition	Ailuropoda melanoleuca E3 ubiquitin/ISG15 ligase TRIM25-like (LOC100477320), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019800285.1

ORF Insert Sequence:

ATGTCAGGGA ACAAAGCTGG GGGCAATCTT CGAGGAGGGG GGGCCAACTT GATAGGGAGA 
TCACAAAACA GCAGCCAAAG AGAAAGGGCA GCCAAGCCTC TAGCCCTCCC TGACATGCAG 
AAAGTTTCAC GGCCAGTCCA GGACCACAAC CCAGCCCTGC AGCCCCCTGC CCAGCCCTCA 
AGACCTCCCC AAACTCAGCT CCTGATGAGT AACAGCAGTC TCCGCACCTT CGAGGACCAA 
GTCCTCTGTC CCATTTGCCT GGAGGTGTTC CGCAATCCGG TCACCACCGC CTGCGGGCAC 
AACTTCTGCA TGACCTGCCT CCAAGGTTTC TGGGACCACC AGGCTACCGT GGGCGAGACG 
CTCTACTGCC CCCAGTGCCG AGAGAGCTTC CCCTCCAGAC CGCACCTCTG CAAGAATGTC 
ATCCTGGAGG AGATGGTGAC CTGCTTCAGC CAGGCCAAGG GCCAGACCTT GGGATCCTCC 
CGGGTCCTGG CCGGGCCCAG GGACGTGCCC TGCGACTTCT GTTCTCCGCA GAAGCTCAAG 
TCAGTCAAGT CCTGTCTGCA GTGCATGGCC TCCCTGTGCG AGGAGCACCT GCGCAGCCAC 
TTCGAGGACC AGGCGTTCCA GGACCACCAG CTGCTGGAGC CCGTGTGGGA TCTCAAGAGC 
CGCCTGTGCC GCAAGCACCG CAAGCTGCGG CGGCTGTACT GCCGCACGGA AGGCTGCTGC 
GTGTGCGGGA CATGTCTGCT GGAGGAGCAC AAGAACCACG ACACCATCCC CCTGGAGGAG 
GAGCGCGCCC ACAAGGAGGT GGAGGTTCGG AAGGTCCAGG CCAATGTGGA AAACCAGATG 
CTGATCATCA ACTCTGACAG CCAGAAGCAC CGGGGGCAGG TGGCCTTTCT CTCGAAACTG 
ATCCAGACAA CACGTGAAGA GGTGAACACC TGCTTCTCAG AGATCATCCA GGAGGTCAAA 
CAGCTGCAGA TGAAGGTCTT GGATTTTGTG GAGAAAGAGG AGGCAGCGGC TCTGGGGAAG 
CTGGGCAGCT CCATCCAGCA GAGCCACAAC CGGCTTCTGA AGCTGGAGGG GGACAGCATC 
TGGCTCCGCA CCCTGCTCGC CAACAGGAGT GACCAGCAGT TTCTGCAGGA GTTCCCCAGG 
CTGAAGCACT TCCCCGCCTG CATGGAACCC CTGATGGGAA CCAACTGCGA GGAGAAGCAG 
GCCTTCCTCC AGTTGCCAGA GACCCTGGCA GAGCTCCGCA CCCGGCTGAT GGATGTGGGT 
CTCAGCTTCA TCAATCAGCT CCTTCTGAAG GGCATTAAGA TGAACTCCTA TGAGGTGCTG 
CCCCCCGCTG TGGACAGGAA AACACTTCTC AAGTGTTACT GCAACCTGAA CTTTGACCCC 
ACCACGGCCA GCGAGGAGCT GTTCCTGTTC AAGGAGACCC ACTCGGTGCT GAACCTGGGC 
ATCCTCCTGG AGCCCTTCGC CACCGGCGGC CCCTTCCCCG GCTTCAAGCA GTGGCCGCAG 
GTGCTTTGCT CGCGCGGCCT GTCCGAGGGC CGCCACTACT GGGAGGCCGA CGTGTCCAAC 
TCGTGGGTGT GCCTGGGCCT CCCTANNCAC ACCTACCGCC GCAGCCCCCC GTTCGGCGGC 
CGCCCGCGCC GCAACATCGT CTACCTGCTG GGCCGCAACC CCTATTCGTG GTGCCTGGAG 
TGGGACTCGC TCAAGTTCTC CGTGTGGCAC AACAACACGC AGACGGTGCT GCACGGCGGC 
TACCACCGCA CGCTCGGCGT GGCGCTCGAC TGNNNNNNNC CCGCCCCCGC CTGCCTCTCC 
TTCTACGGCG TGGCGGGCGG CGTGAGCCTG CTCTACCGCT TCCTCGTCTC CTTCCTGGAG 
CCGCTCTACC CCGCAGTCAT GGTCAGCAGC GGCGCCTCGG TCACGCTCAA GCAGCGCCCG 
GAGGCGGAGG CGGCGGCGAG GCAGACCCCT CGGTTCCCGC AGCCGCAGGA GAAGGGCTCT 
GAGCCGGCCT GGAGTTCCCC CCGGGACTGG GGACAGTCCG GTGGCCTCCG GAGGGCGGAT 
TCCTGGCCCG GGTGCACAGT CTCCCCCACC GTGGCGACCA CCCTCCTAAG CCCCACCAGA 
GGCCCCTGGC CTTGGTTGTC CAGGCTGGTG CCGGCTACAC AGCAACGTGT GGCCTGA