DYSF gene cDNA ORF clone, Ailuropoda melanoleuca(giant panda)

mRNA	Protein	Name
XM_019794823.1	XP_019650382.1	dysferlin isoform X4
XM_019794826.1	XP_019650385.1	dysferlin isoform X9
XM_019794827.1	XP_019650386.1	dysferlin isoform X10
XM_019794830.1	XP_019650389.1	dysferlin isoform X13
XM_019794828.1	XP_019650387.1	dysferlin isoform X11
XM_002914885.3	XP_002914931.1	dysferlin isoform X15
XM_019794832.1	XP_019650391.1	dysferlin isoform X19
XM_002914894.3	XP_002914940.1	dysferlin isoform X20
XM_019794820.1	XP_019650379.1	dysferlin isoform X1
XM_019794822.1	XP_019650381.1	dysferlin isoform X3
XM_002914892.3	XP_002914938.1	dysferlin isoform X14
XM_019794831.1	XP_019650390.1	dysferlin isoform X18
XM_019794821.1	XP_019650380.1	dysferlin isoform X2
XM_019794824.1	XP_019650383.1	dysferlin isoform X5
XM_002914890.3	XP_002914936.1	dysferlin isoform X6
XM_002914883.3	XP_002914929.1	dysferlin isoform X7
XM_002914893.3	XP_002914939.1	dysferlin isoform X16
XM_019794825.1	XP_019650384.1	dysferlin isoform X8
XM_019794829.1	XP_019650388.1	dysferlin isoform X12
XM_002914886.3	XP_002914932.1	dysferlin isoform X17
XM_002914887.3	XP_002914933.1	dysferlin isoform X21

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAc89674	XM_019794820.1 Latest version!	Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OAc89675	XM_019794821.1 Latest version!	Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OAc89676	XM_019794822.1 Latest version!	Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OAc89677	XM_019794823.1 Latest version!	Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OAc89678	XM_019794824.1 Latest version!	Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OAc89679	XM_019794825.1 Latest version!	Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OAc89680	XM_019794826.1 Latest version!	Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OAc89681	XM_019794827.1 Latest version!	Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OAc89682	XM_019794828.1 Latest version!	Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OAc89683	XM_019794829.1 Latest version!	Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OAc89684	XM_019794830.1 Latest version!	Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OAc89685	XM_019794831.1 Latest version!	Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OAc89686	XM_019794832.1 Latest version!	Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OAc02550	XM_002914883.3 Latest version!	Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OAc02552	XM_002914885.3 Latest version!	Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OAc02553	XM_002914886.3 Latest version!	Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OAc02554	XM_002914887.3 Latest version!	Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OAc02557	XM_002914890.3 Latest version!	Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OAc02559	XM_002914892.3 Latest version!	Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OAc02560	XM_002914893.3 Latest version!	Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OAc02561	XM_002914894.3 Latest version!	Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

DYSF ( XM_019794820.1 ) cDNA ORF clone, Ailuropoda melanoleuca(giant panda) -> XP_019650379.1 Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAc89674
Clone ID Related Accession (Same CDS sequence)	XM_019794820.1
Accession Version	XM_019794820.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6450bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-27
Organism	Ailuropoda melanoleuca(giant panda)
Product	dysferlin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003217356.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ailuropoda melanoleuca Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGTGAAGAA GGACCGGCGC 
AGTGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCAGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTCCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCGG GAGGTCCTCG CCACCCCCAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCCTC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGTGGCTG GTGGGGGACA GAGCCGGGCT 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACGGGAGAT 
GAGGCAGAGC CATTACTGGA TCCGAGCGGT GCCCCAGGCC CTGGGGCCCC CACCACCCCG 
AGGAAGCCAC CTTCCCATCC GCCCCCCTAC CACCCCGGGA GCAAGAGAAA AAGAGGTGCT 
CCCACGCCCA GAAAGCTGCT GTCGGACAAA CCGCAGGACT TCCAGATCAG GGTGCAAGTG 
ATCGAGGGGC GCCAGCTGCC AGGGGTGAAT ATCAAGCCTG TGGTCAAAGT CACGGCTGCC 
GGGCAGACCA AGAGGACACG GATCCACAAG GGAAACAGCC CACTCTTCAA CGAGACTCTC 
TTCTTCAATG TGTTTGACTC TCCCTCGGAG CTGTTTGACG AGCCCATCTT TATCACGGTG 
GTAGACTCCC GTTCTCTCCG GACAGACGCT CTCATTGGGG AATTCCGGAT GGACGTGGGT 
ACCATCTACA GAGAGCCCCG ACACGCCTAT CTGAGGAAAT GGCTTCTCCT CTCGGACCCT 
GACGATTTCT CTGCTGGGGC CAGAGGTTAC CTGAAAGCAA GCCTCTGTGT GCTGGGGCCC 
GGCGATGAAG CGCCTCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATCGAAAAC 
AATCTGCTCA GGCCAACTGG CGTGGCCCTT CGAGGAGCCC ACTTCTGCCT GAAGGTCTTC 
AGAGCTGAGG ACTTACCACA GATGGATGAT GCCGTGATGG ACAACGTGAA GCAGATCTTC 
GGCTTTGACA GTAACAAGAA GAACTTGGTG GATCCCTTCA TGGAAGTCAG CTTTGCGGGG 
AAAATGCTCT GCAGCAAGAT CCTGGAGAAA ACAGCCAACC CTCAGTGGAA CCAGAGCATC 
ACGCTGCCCG CCATGTTTCC CTCCATGTGT GAAAAAATGA GGATTCGCGT CATGGACTGG 
GACCGCCTCA CCCACAATGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCC 
GCCCCTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTGG CCAAGCCTTC TCCAGCCATG 
GACCTGGACG ACCATCTGGG CTTCCTCCCC ACCTTCGGGC CCTGCTACGT CAACCTCTAC 
GGCAGCCCCA GGGAGTTCAC CGGCTTCCCG GACCCCTACG CAGAACTCAA CATGGGCAAG 
GGAGAAGGTG TGGCCTACCG AGGTCGGCTT CTGCTCTCCC TGGAGACCAA GCTGGTGGAG 
CACAGCGACC AAAAGGTGGA GGACCTCCCT GCGGATGACA TCCTTCGGGT GGAGAAGTAT 
CTCCGGAGGC GTAAGTACTC CCTCTTTGCC GCCTTCTACT CGGCCTCCAT GCTCCAGGAT 
GTGGATGATG CCATCCAGTT CGAGGTCAGC ATCGGGAACT ATGGCAACAA GTTCGACACC 
ACGTGCCTGC CGCTGGCCTC TACCACCCAG TACAGCCGGG CGGTCTTTGA CGGGTGCCAC 
TACTACTACT TGCCCTGGGG CAACGTGAAG CCCGTGGTGG TGCTGTCATC CTACTGGGAG 
GACATCAGCC ACAGAGTTGA GGCTCAGAAC CAGCTGCTCA GGATTGCGGA CCGGCTGGAA 
GCTGCCCTGG AGCAAGTCCA CTTGGCCGTG AAGGCGCAGT ACAACGCCGA GGACGTGGAC 
TCCCTGTTGG CTCAGCTGAT GGATGAGCTC ATCTCAGACT GCAGCCAGCC CCTAGGCGAT 
GTCCACGAGA CACCCTCTGC CACCCACCTG GACCAGTACC TGCACAGGCT GCGTAGCCGT 
CACCTAAGCC AGATTGCAGA GGCGGCGCTG GCCCTGAAGC TCGGGCACGG CGGGCTCCCG 
GCCGCCCTGG AGCAGGCTGA AGACTGGCTC CTGCGGCTCC GTGCCCTGGC CGAGGAGCCT 
CAGAACAGCC TGCCCGACAT CATCATCTGG ATGCTGCAGG GCGACAAGCG AGTGGCATAC 
CAGCGGGTGC CTACACATGA GGTCCTCTTC TCCCGGCGTG GAGCCAGCTA CTGCGGCAAG 
AACTGTGGGA AGCTGCAGAC GATATTTCTC AAATATCCCA TGCAGGGGGT GCCCGGAGCC 
CGGATGCCCG TGCAGATACG GGTGAAGCTC TGGTTTGGGC TTTCTGTGGA TGAGAAGGAG 
TTCAACCAGT TTGCTGAGGG AAAGCTGTCT GTCTTTGCTG AAACCTATGA GAACCAGACC 
AAACTGGCCC TCGTTGGGAA CTGGGGCACA ACAGGCCTTA CCTACCCCAA GTTTTCTGAT 
GTCGCGGGCA AGATCAAGCT CCCCAAGGAT AGCTTCCGCC CCTCAGCCGG CTGGACCTGG 
GCTGGAGATT GGTTTGTGTG TCCAGAGAAG ACGCTGCTCC ATGACACCGA CGCGGGCCAC 
CTGAGTTTCG TGGCGGAGGT GTTTGAGAAC CAGACCCGGC TCCCTGGAGG CCACTGGGTC 
TACATGAGTG ACAACTACAC GGATGTGAAT GGGGAGAAGG TGCTTCCCAA GGATGACATT 
GAGTGCCCGC TGGGCTGGAA GTGGGAAGAT GAAGAGTGGT CCACGGACCT CAATCGGGCC 
GTTGATGAGC AAGGCTGGGA GTACAGTATC ACCATCCCCC CGGACCGGAA GCCGAAGCAC 
TGGGTCCCCG CTGAGAAGAT GTACTACACG CACCGTCGGC GGCGCTGGGT CCGCCTGCGC 
AGGAGGGACC TCAGCCAGAT GGAGGCCCTG AGGAAGCACA GGCAGGCGGA GGCGGAGGGC 
GAGGGCTGGG AGTACGCCTC GCTCTTTGGC TGGAAATTCC ACCTGGAGTA CCGCAAAACG 
GATGCATTCC GTCGCCGCCG CTGGCGCCGC CGCATGGAGC CGCTGGAGAA GACGGGGCCT 
GCAGCCGTGT TTGCCCTTGA GGGGGCACTG GGTGGCGTGG TAGATGACCG GAGTGAAGAT 
TCCATGTCCG TGTCCACCTT GAGCTTTGGC GTGAACAGAC CCACGATTTC CTGCATCTTT 
GATCATGGGA ACCGCTACCA CCTGCGCTGC TATATGTACC AGGCTCGGGA CCTGCCCTCG 
ATGGACAAGG ACTCTTTTTC TGATCCCTAC GCCATCGTCT CCTTCCTGCA CCAGAGCCAG 
AAGACCGTGG TGGTGAAGAA CACTCTGAAC CCCACCTGGG ACCAGACGCT CATCTTCTAT 
GAGATTGAGA TCTTTGGAGA GCCAGCCAGC ATCGCCGAGC AGCCGCCCAG CATTGTGGTG 
GAGCTGTATG ACCACGACAC CTACGGTGCA GATGAGTTTT TGGGACGCTG CATGTGTCAG 
CCCAGTCTGG AACGGATGCC CCGGCTGGCC TGGTTCCCGC TGGTTAGGGG CAGCCAGCCG 
GCAGGCGAGC TGCTGGCCTC TTTTGAGCTC ATCCAGAGAG AGAAGCCGGC CATTCACCAT 
ATTCCTGGTT TTGAGGTGCA GGATACATCG GGAATCCTGG ACGAGCTGTG TGCACCTGCC 
TTCTTCCTCC AGGGCCTCCT CCAAACGTCG GAGGACACAG ACCTGCCCTA CCCACCGCCT 
CAGAGGGAGG CCAACATCTA CATGGTTCCC CAGAACATCA AGCCGGTTCT CCAGCGCACT 
GCCATAGAGA TCCTGGCATG GGGCCTACGG AACATGAAGA GTTACCAGCT GGCCAGTATC 
TCCTCCCCCA GCCTCGTGGT GGAGTGCGGG GGCCAGACGG TGCAGTCCTG TGTCATCAGG 
AACCTCCGGA AGAACCCCAA CTTCGACATC TGCACCCTCT TCATGGAAGT GATGCTGCCC 
AGAGAGGAGC TCTACTGTCC CCCCATCGTG ATCAAGGTCA TTGATAACCG CCAGTTTGGC 
CGCCGGCCTG TGGTGGGCCA GTGTACCATA CGCTCCCTGG AGAGCTTCAT GTGTGACCTG 
TACTCCGAGG AGAGTCCGTC CCCACAGGGC GGCCCAGATG ATGTGAGTTT ACTCAGTCCT 
GGGGAAGACG TGCTCATTGA CATCGACGAC AAGGAGCCCC TCATCCCCAT ACAGCTTACA 
GACGGTCTGT TGGGCTCTGC CCCCACTAAC ATGGCATCCT CTCCATCCAG TCTTCATGAG 
GAGGAGTTCA TCGACTGGTG GAGCAAATTC TTTGCCTCCA CAGGGGAGAG GGAGAAGTGC 
GGCTCGTACT TGGAGAAGGG CTTTGACACT CTAAAGGTCT ATGACACACA ACTGGAGAAC 
GTGGAGGCCT TTGGGGGCCT GTCTGACTTT TGTAACACCT TCAAGCTCTA CCGGGGCAAG 
ACTCAGGAGG AGACGGAGGA CCCATCTGTT ATCGGGGAGT TTAAGGGCCT CTTCAAAATC 
TACCCCCTCC CGGAAGACCC GGCCGTCCCC ATGCCCCCGA GACAGTTCCA GCAGCTGGCT 
GCCCAGGGGC CTCAGGACTG CCTGGTCCGC ATCTATATCA TCCGGGCATT TGGCCTGCAG 
CCCAAGGACC CCAATGGGAA GTGTGATCCT TACATCAAGA TCTCCATAGG GAAGAAATCA 
GTGAGTGACC AGGATAATTA CATTCCCTGT ACCCTGGAGC CTGTGTTTGG AAAGATGTTC 
GAGCTGACCT GCACTCTGCC TCTGGAGAAG GACCTGAAGA TCACACTCTA CGACTATGAC 
CTCCTCTCCA AAGATGAGAA GATCGGGGAG ACAGTCATCG ACCTGGAGAA TAGGCTGCTG 
TCCAAGTTCG GGGCTCGCTG CGGACTCCCA CAGACCTACT GTGTCTCTGG ACCCAACCAG 
TGGAGGGACC AGCTCCGCCC CTCCCAGCTT CTCCAGCTCA CCTGCCAGCA GCGCAGACTC 
AAGGCGCCCG TGTACCTCAC AGACCGCGTA CTCTTTCAGG ATAAAGAATA CACCATTGAG 
GAAATAGAGG CTGGCAGGGT CCTGAACCCA CACCTGGGCC CAGTGGAAGA GCGTCTGGCC 
TTGCACGTCC TCCAGCAGCT GGGCCTGGTC CCCGAGCATG TGGAGTCCCG GCCTCTTTAC 
AGCCCCCTGC AGCCAGACAT TGAGCAGGGG AAGCTGCAGA TGTGGATCGA TCTGTTTCCC 
AAGGCCCTGG GGCGACCTGG ACCTCCCTTC AACATCACCC CACGGAGAGC AAGAAGGTTT 
TTCCTGCGTT GTATTATCTG GAACACAAAG GACGTGATCC TGGACGACCT CAGCATCACG 
GGGGAGAAGA TGAGTGACAT TTACGTGAAA GGTTGGATGG TCGGCTTTGA AGAGCACAAG 
CAGAAGACGG ACGTGCACTA CCGCTCCCTG GGAGGCGAAG GCAACTTTAA CTGGAGGTTC 
ATTTTCCCCT TTGACTACCT GCCCGCTGAG CAAGTCTGCA CCGTCTCTAA GAAGGATGCT 
TTCTGGAGGC TGGACAAGAC TGAGAGCAAA ATCCCAGCAC AAGTGGTATT TCAGATCTGG 
GACAATGACA AGTTCTCCTT TGACGACTTT CTGGGCTCGT TGCAGTTGGA TCTCAACCGC 
ATGCCCAAGC CTGCCAAGAC AGCTGAGAAG TGCTCCTTGG ACCAGCTGGA CGACACTTTC 
CACCCGGAGT GGTTTGTGTC CCTTTTTGAA CAGAAAACAG TAAAGGGCTG GTGGCCCTGT 
GTGGCAGAAG ACGGTGAGAA GAAGATCCTG GCGGGCAAGC TGGAAATGAC CTTGGAGATC 
GTAGCAGAGA GTGAGCACGA AGAGCGGCCT GCGGGTCAAG GCCGGGATGA ACCCAACATG 
AACCCCAAGC TCGAGGACCC AAGGCGCCCC GACACCTCCT TCCTGTGGTT CACCTCTCCG 
TACAAGACCA TGAAGTTCAT CCTGTGGCGG CGGTTCCGGT GCGCCATCAT CCTCTTCGTC 
GTTCTCGTCA TCCTGCTGCT CTTCCTGGGC ATCTTCCTCT ATTCCTTCCC GAACTATGCT 
GCCATGAAGC TGGTGAAACC CTTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019650379.1
CDS	150..6599
Translation	MLSCLLVRASNLPSVKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEAQVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPSASLEPSPTLPDMDMVAGGGQSRAETWSLLSDSTVDTRYSGKKWLAPTDTGGEEDTEDQGLTGDEAEPLLDPSGAPGPGAPTTPRKPPSHPPPYHPGSKRKRGAPTPRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVMDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGVAKPSPAMDLDDHLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRLLLSLETKLVEHSDQKVEDLPADDILRVEKYLRRRKYSLFAAFYSASMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADRLEAALEQVHLAVKAQYNAEDVDSLLAQLMDELISDCSQPLGDVHETPSATHLDQYLHRLRSRHLSQIAEAALALKLGHGGLPAALEQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPTHEVLFSRRGASYCGKNCGKLQTIFLKYPMQGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVAGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDTDAGHLSFVAEVFENQTRLPGGHWVYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALRKHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSMSVSTLSFGVNRPTISCIFDHGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFLGRCMCQPSLERMPRLAWFPLVRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTSGILDELCAPAFFLQGLLQTSEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPIVIKVIDNRQFGRRPVVGQCTIRSLESFMCDLYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLTDGLLGSAPTNMASSPSSLHEEEFIDWWSKFFASTGEREKCGSYLEKGFDTLKVYDTQLENVEAFGGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAVPMPPRQFQQLAAQGPQDCLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLQLTCQQRRLKAPVYLTDRVLFQDKEYTIEEIEAGRVLNPHLGPVEERLALHVLQQLGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPAQVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEDGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFVVLVILLLFLGIFLYSFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_019794820.1
Organism	Ailuropoda melanoleuca(giant panda)
Definition	Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019794820.1

ORF Insert Sequence:

ATGCTGAGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGTGAAGAA GGACCGGCGC 
AGTGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCAGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTCCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCGG GAGGTCCTCG CCACCCCCAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCCTC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGTGGCTG GTGGGGGACA GAGCCGGGCT 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACGGGAGAT 
GAGGCAGAGC CATTACTGGA TCCGAGCGGT GCCCCAGGCC CTGGGGCCCC CACCACCCCG 
AGGAAGCCAC CTTCCCATCC GCCCCCCTAC CACCCCGGGA GCAAGAGAAA AAGAGGTGCT 
CCCACGCCCA GAAAGCTGCT GTCGGACAAA CCGCAGGACT TCCAGATCAG GGTGCAAGTG 
ATCGAGGGGC GCCAGCTGCC AGGGGTGAAT ATCAAGCCTG TGGTCAAAGT CACGGCTGCC 
GGGCAGACCA AGAGGACACG GATCCACAAG GGAAACAGCC CACTCTTCAA CGAGACTCTC 
TTCTTCAATG TGTTTGACTC TCCCTCGGAG CTGTTTGACG AGCCCATCTT TATCACGGTG 
GTAGACTCCC GTTCTCTCCG GACAGACGCT CTCATTGGGG AATTCCGGAT GGACGTGGGT 
ACCATCTACA GAGAGCCCCG ACACGCCTAT CTGAGGAAAT GGCTTCTCCT CTCGGACCCT 
GACGATTTCT CTGCTGGGGC CAGAGGTTAC CTGAAAGCAA GCCTCTGTGT GCTGGGGCCC 
GGCGATGAAG CGCCTCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATCGAAAAC 
AATCTGCTCA GGCCAACTGG CGTGGCCCTT CGAGGAGCCC ACTTCTGCCT GAAGGTCTTC 
AGAGCTGAGG ACTTACCACA GATGGATGAT GCCGTGATGG ACAACGTGAA GCAGATCTTC 
GGCTTTGACA GTAACAAGAA GAACTTGGTG GATCCCTTCA TGGAAGTCAG CTTTGCGGGG 
AAAATGCTCT GCAGCAAGAT CCTGGAGAAA ACAGCCAACC CTCAGTGGAA CCAGAGCATC 
ACGCTGCCCG CCATGTTTCC CTCCATGTGT GAAAAAATGA GGATTCGCGT CATGGACTGG 
GACCGCCTCA CCCACAATGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCC 
GCCCCTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTGG CCAAGCCTTC TCCAGCCATG 
GACCTGGACG ACCATCTGGG CTTCCTCCCC ACCTTCGGGC CCTGCTACGT CAACCTCTAC 
GGCAGCCCCA GGGAGTTCAC CGGCTTCCCG GACCCCTACG CAGAACTCAA CATGGGCAAG 
GGAGAAGGTG TGGCCTACCG AGGTCGGCTT CTGCTCTCCC TGGAGACCAA GCTGGTGGAG 
CACAGCGACC AAAAGGTGGA GGACCTCCCT GCGGATGACA TCCTTCGGGT GGAGAAGTAT 
CTCCGGAGGC GTAAGTACTC CCTCTTTGCC GCCTTCTACT CGGCCTCCAT GCTCCAGGAT 
GTGGATGATG CCATCCAGTT CGAGGTCAGC ATCGGGAACT ATGGCAACAA GTTCGACACC 
ACGTGCCTGC CGCTGGCCTC TACCACCCAG TACAGCCGGG CGGTCTTTGA CGGGTGCCAC 
TACTACTACT TGCCCTGGGG CAACGTGAAG CCCGTGGTGG TGCTGTCATC CTACTGGGAG 
GACATCAGCC ACAGAGTTGA GGCTCAGAAC CAGCTGCTCA GGATTGCGGA CCGGCTGGAA 
GCTGCCCTGG AGCAAGTCCA CTTGGCCGTG AAGGCGCAGT ACAACGCCGA GGACGTGGAC 
TCCCTGTTGG CTCAGCTGAT GGATGAGCTC ATCTCAGACT GCAGCCAGCC CCTAGGCGAT 
GTCCACGAGA CACCCTCTGC CACCCACCTG GACCAGTACC TGCACAGGCT GCGTAGCCGT 
CACCTAAGCC AGATTGCAGA GGCGGCGCTG GCCCTGAAGC TCGGGCACGG CGGGCTCCCG 
GCCGCCCTGG AGCAGGCTGA AGACTGGCTC CTGCGGCTCC GTGCCCTGGC CGAGGAGCCT 
CAGAACAGCC TGCCCGACAT CATCATCTGG ATGCTGCAGG GCGACAAGCG AGTGGCATAC 
CAGCGGGTGC CTACACATGA GGTCCTCTTC TCCCGGCGTG GAGCCAGCTA CTGCGGCAAG 
AACTGTGGGA AGCTGCAGAC GATATTTCTC AAATATCCCA TGCAGGGGGT GCCCGGAGCC 
CGGATGCCCG TGCAGATACG GGTGAAGCTC TGGTTTGGGC TTTCTGTGGA TGAGAAGGAG 
TTCAACCAGT TTGCTGAGGG AAAGCTGTCT GTCTTTGCTG AAACCTATGA GAACCAGACC 
AAACTGGCCC TCGTTGGGAA CTGGGGCACA ACAGGCCTTA CCTACCCCAA GTTTTCTGAT 
GTCGCGGGCA AGATCAAGCT CCCCAAGGAT AGCTTCCGCC CCTCAGCCGG CTGGACCTGG 
GCTGGAGATT GGTTTGTGTG TCCAGAGAAG ACGCTGCTCC ATGACACCGA CGCGGGCCAC 
CTGAGTTTCG TGGCGGAGGT GTTTGAGAAC CAGACCCGGC TCCCTGGAGG CCACTGGGTC 
TACATGAGTG ACAACTACAC GGATGTGAAT GGGGAGAAGG TGCTTCCCAA GGATGACATT 
GAGTGCCCGC TGGGCTGGAA GTGGGAAGAT GAAGAGTGGT CCACGGACCT CAATCGGGCC 
GTTGATGAGC AAGGCTGGGA GTACAGTATC ACCATCCCCC CGGACCGGAA GCCGAAGCAC 
TGGGTCCCCG CTGAGAAGAT GTACTACACG CACCGTCGGC GGCGCTGGGT CCGCCTGCGC 
AGGAGGGACC TCAGCCAGAT GGAGGCCCTG AGGAAGCACA GGCAGGCGGA GGCGGAGGGC 
GAGGGCTGGG AGTACGCCTC GCTCTTTGGC TGGAAATTCC ACCTGGAGTA CCGCAAAACG 
GATGCATTCC GTCGCCGCCG CTGGCGCCGC CGCATGGAGC CGCTGGAGAA GACGGGGCCT 
GCAGCCGTGT TTGCCCTTGA GGGGGCACTG GGTGGCGTGG TAGATGACCG GAGTGAAGAT 
TCCATGTCCG TGTCCACCTT GAGCTTTGGC GTGAACAGAC CCACGATTTC CTGCATCTTT 
GATCATGGGA ACCGCTACCA CCTGCGCTGC TATATGTACC AGGCTCGGGA CCTGCCCTCG 
ATGGACAAGG ACTCTTTTTC TGATCCCTAC GCCATCGTCT CCTTCCTGCA CCAGAGCCAG 
AAGACCGTGG TGGTGAAGAA CACTCTGAAC CCCACCTGGG ACCAGACGCT CATCTTCTAT 
GAGATTGAGA TCTTTGGAGA GCCAGCCAGC ATCGCCGAGC AGCCGCCCAG CATTGTGGTG 
GAGCTGTATG ACCACGACAC CTACGGTGCA GATGAGTTTT TGGGACGCTG CATGTGTCAG 
CCCAGTCTGG AACGGATGCC CCGGCTGGCC TGGTTCCCGC TGGTTAGGGG CAGCCAGCCG 
GCAGGCGAGC TGCTGGCCTC TTTTGAGCTC ATCCAGAGAG AGAAGCCGGC CATTCACCAT 
ATTCCTGGTT TTGAGGTGCA GGATACATCG GGAATCCTGG ACGAGCTGTG TGCACCTGCC 
TTCTTCCTCC AGGGCCTCCT CCAAACGTCG GAGGACACAG ACCTGCCCTA CCCACCGCCT 
CAGAGGGAGG CCAACATCTA CATGGTTCCC CAGAACATCA AGCCGGTTCT CCAGCGCACT 
GCCATAGAGA TCCTGGCATG GGGCCTACGG AACATGAAGA GTTACCAGCT GGCCAGTATC 
TCCTCCCCCA GCCTCGTGGT GGAGTGCGGG GGCCAGACGG TGCAGTCCTG TGTCATCAGG 
AACCTCCGGA AGAACCCCAA CTTCGACATC TGCACCCTCT TCATGGAAGT GATGCTGCCC 
AGAGAGGAGC TCTACTGTCC CCCCATCGTG ATCAAGGTCA TTGATAACCG CCAGTTTGGC 
CGCCGGCCTG TGGTGGGCCA GTGTACCATA CGCTCCCTGG AGAGCTTCAT GTGTGACCTG 
TACTCCGAGG AGAGTCCGTC CCCACAGGGC GGCCCAGATG ATGTGAGTTT ACTCAGTCCT 
GGGGAAGACG TGCTCATTGA CATCGACGAC AAGGAGCCCC TCATCCCCAT ACAGCTTACA 
GACGGTCTGT TGGGCTCTGC CCCCACTAAC ATGGCATCCT CTCCATCCAG TCTTCATGAG 
GAGGAGTTCA TCGACTGGTG GAGCAAATTC TTTGCCTCCA CAGGGGAGAG GGAGAAGTGC 
GGCTCGTACT TGGAGAAGGG CTTTGACACT CTAAAGGTCT ATGACACACA ACTGGAGAAC 
GTGGAGGCCT TTGGGGGCCT GTCTGACTTT TGTAACACCT TCAAGCTCTA CCGGGGCAAG 
ACTCAGGAGG AGACGGAGGA CCCATCTGTT ATCGGGGAGT TTAAGGGCCT CTTCAAAATC 
TACCCCCTCC CGGAAGACCC GGCCGTCCCC ATGCCCCCGA GACAGTTCCA GCAGCTGGCT 
GCCCAGGGGC CTCAGGACTG CCTGGTCCGC ATCTATATCA TCCGGGCATT TGGCCTGCAG 
CCCAAGGACC CCAATGGGAA GTGTGATCCT TACATCAAGA TCTCCATAGG GAAGAAATCA 
GTGAGTGACC AGGATAATTA CATTCCCTGT ACCCTGGAGC CTGTGTTTGG AAAGATGTTC 
GAGCTGACCT GCACTCTGCC TCTGGAGAAG GACCTGAAGA TCACACTCTA CGACTATGAC 
CTCCTCTCCA AAGATGAGAA GATCGGGGAG ACAGTCATCG ACCTGGAGAA TAGGCTGCTG 
TCCAAGTTCG GGGCTCGCTG CGGACTCCCA CAGACCTACT GTGTCTCTGG ACCCAACCAG 
TGGAGGGACC AGCTCCGCCC CTCCCAGCTT CTCCAGCTCA CCTGCCAGCA GCGCAGACTC 
AAGGCGCCCG TGTACCTCAC AGACCGCGTA CTCTTTCAGG ATAAAGAATA CACCATTGAG 
GAAATAGAGG CTGGCAGGGT CCTGAACCCA CACCTGGGCC CAGTGGAAGA GCGTCTGGCC 
TTGCACGTCC TCCAGCAGCT GGGCCTGGTC CCCGAGCATG TGGAGTCCCG GCCTCTTTAC 
AGCCCCCTGC AGCCAGACAT TGAGCAGGGG AAGCTGCAGA TGTGGATCGA TCTGTTTCCC 
AAGGCCCTGG GGCGACCTGG ACCTCCCTTC AACATCACCC CACGGAGAGC AAGAAGGTTT 
TTCCTGCGTT GTATTATCTG GAACACAAAG GACGTGATCC TGGACGACCT CAGCATCACG 
GGGGAGAAGA TGAGTGACAT TTACGTGAAA GGTTGGATGG TCGGCTTTGA AGAGCACAAG 
CAGAAGACGG ACGTGCACTA CCGCTCCCTG GGAGGCGAAG GCAACTTTAA CTGGAGGTTC 
ATTTTCCCCT TTGACTACCT GCCCGCTGAG CAAGTCTGCA CCGTCTCTAA GAAGGATGCT 
TTCTGGAGGC TGGACAAGAC TGAGAGCAAA ATCCCAGCAC AAGTGGTATT TCAGATCTGG 
GACAATGACA AGTTCTCCTT TGACGACTTT CTGGGCTCGT TGCAGTTGGA TCTCAACCGC 
ATGCCCAAGC CTGCCAAGAC AGCTGAGAAG TGCTCCTTGG ACCAGCTGGA CGACACTTTC 
CACCCGGAGT GGTTTGTGTC CCTTTTTGAA CAGAAAACAG TAAAGGGCTG GTGGCCCTGT 
GTGGCAGAAG ACGGTGAGAA GAAGATCCTG GCGGGCAAGC TGGAAATGAC CTTGGAGATC 
GTAGCAGAGA GTGAGCACGA AGAGCGGCCT GCGGGTCAAG GCCGGGATGA ACCCAACATG 
AACCCCAAGC TCGAGGACCC AAGGCGCCCC GACACCTCCT TCCTGTGGTT CACCTCTCCG 
TACAAGACCA TGAAGTTCAT CCTGTGGCGG CGGTTCCGGT GCGCCATCAT CCTCTTCGTC 
GTTCTCGTCA TCCTGCTGCT CTTCCTGGGC ATCTTCCTCT ATTCCTTCCC GAACTATGCT 
GCCATGAAGC TGGTGAAACC CTTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_019794821.1 ) cDNA ORF clone, Ailuropoda melanoleuca(giant panda) -> XP_019650380.1 Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X2, mRNA.

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CloneID	OAc89675
Clone ID Related Accession (Same CDS sequence)	XM_019794821.1
Accession Version	XM_019794821.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6447bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-27
Organism	Ailuropoda melanoleuca(giant panda)
Product	dysferlin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003217356.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ailuropoda melanoleuca Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTATGCCGAG AATGTCCACA CCCCGGACAC CGACATCAGC 
GATGCCTACT GCTCTGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CAGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGAGCTCCG AGCTCCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
TTAGGGGAAG CCCAAGTCCC ACTCCGGGAG GTCCTCGCCA CCCCCAGTCT GTCTGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CGCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCCTCTGC ATCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATATG GTGGCTGGTG GGGGACAGAG CCGGGCTGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCTG 
GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAGGACC AGGGGCTCAC GGGAGATGAG 
GCAGAGCCAT TACTGGATCC GAGCGGTGCC CCAGGCCCTG GGGCCCCCAC CACCCCGAGG 
AAGCCACCTT CCCATCCGCC CCCCTACCAC CCCGGGAGCA AGAGAAAAAG AGGTGCTCCC 
ACGCCCAGAA AGCTGCTGTC GGACAAACCG CAGGACTTCC AGATCAGGGT GCAAGTGATC 
GAGGGGCGCC AGCTGCCAGG GGTGAATATC AAGCCTGTGG TCAAAGTCAC GGCTGCCGGG 
CAGACCAAGA GGACACGGAT CCACAAGGGA AACAGCCCAC TCTTCAACGA GACTCTCTTC 
TTCAATGTGT TTGACTCTCC CTCGGAGCTG TTTGACGAGC CCATCTTTAT CACGGTGGTA 
GACTCCCGTT CTCTCCGGAC AGACGCTCTC ATTGGGGAAT TCCGGATGGA CGTGGGTACC 
ATCTACAGAG AGCCCCGACA CGCCTATCTG AGGAAATGGC TTCTCCTCTC GGACCCTGAC 
GATTTCTCTG CTGGGGCCAG AGGTTACCTG AAAGCAAGCC TCTGTGTGCT GGGGCCCGGC 
GATGAAGCGC CTCTGGAGAG AAAGGACCCC TCTGAAGACA AGGAGGACAT CGAAAACAAT 
CTGCTCAGGC CAACTGGCGT GGCCCTTCGA GGAGCCCACT TCTGCCTGAA GGTCTTCAGA 
GCTGAGGACT TACCACAGAT GGATGATGCC GTGATGGACA ACGTGAAGCA GATCTTCGGC 
TTTGACAGTA ACAAGAAGAA CTTGGTGGAT CCCTTCATGG AAGTCAGCTT TGCGGGGAAA 
ATGCTCTGCA GCAAGATCCT GGAGAAAACA GCCAACCCTC AGTGGAACCA GAGCATCACG 
CTGCCCGCCA TGTTTCCCTC CATGTGTGAA AAAATGAGGA TTCGCGTCAT GGACTGGGAC 
CGCCTCACCC ACAATGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCCGCC 
CCTGGAGGAG AAATAGAAGA GGAGCCTGCA GGTGTGGCCA AGCCTTCTCC AGCCATGGAC 
CTGGACGACC ATCTGGGCTT CCTCCCCACC TTCGGGCCCT GCTACGTCAA CCTCTACGGC 
AGCCCCAGGG AGTTCACCGG CTTCCCGGAC CCCTACGCAG AACTCAACAT GGGCAAGGGA 
GAAGGTGTGG CCTACCGAGG TCGGCTTCTG CTCTCCCTGG AGACCAAGCT GGTGGAGCAC 
AGCGACCAAA AGGTGGAGGA CCTCCCTGCG GATGACATCC TTCGGGTGGA GAAGTATCTC 
CGGAGGCGTA AGTACTCCCT CTTTGCCGCC TTCTACTCGG CCTCCATGCT CCAGGATGTG 
GATGATGCCA TCCAGTTCGA GGTCAGCATC GGGAACTATG GCAACAAGTT CGACACCACG 
TGCCTGCCGC TGGCCTCTAC CACCCAGTAC AGCCGGGCGG TCTTTGACGG GTGCCACTAC 
TACTACTTGC CCTGGGGCAA CGTGAAGCCC GTGGTGGTGC TGTCATCCTA CTGGGAGGAC 
ATCAGCCACA GAGTTGAGGC TCAGAACCAG CTGCTCAGGA TTGCGGACCG GCTGGAAGCT 
GCCCTGGAGC AAGTCCACTT GGCCGTGAAG GCGCAGTACA ACGCCGAGGA CGTGGACTCC 
CTGTTGGCTC AGCTGATGGA TGAGCTCATC TCAGACTGCA GCCAGCCCCT AGGCGATGTC 
CACGAGACAC CCTCTGCCAC CCACCTGGAC CAGTACCTGC ACAGGCTGCG TAGCCGTCAC 
CTAAGCCAGA TTGCAGAGGC GGCGCTGGCC CTGAAGCTCG GGCACGGCGG GCTCCCGGCC 
GCCCTGGAGC AGGCTGAAGA CTGGCTCCTG CGGCTCCGTG CCCTGGCCGA GGAGCCTCAG 
AACAGCCTGC CCGACATCAT CATCTGGATG CTGCAGGGCG ACAAGCGAGT GGCATACCAG 
CGGGTGCCTA CACATGAGGT CCTCTTCTCC CGGCGTGGAG CCAGCTACTG CGGCAAGAAC 
TGTGGGAAGC TGCAGACGAT ATTTCTCAAA TATCCCATGC AGGGGGTGCC CGGAGCCCGG 
ATGCCCGTGC AGATACGGGT GAAGCTCTGG TTTGGGCTTT CTGTGGATGA GAAGGAGTTC 
AACCAGTTTG CTGAGGGAAA GCTGTCTGTC TTTGCTGAAA CCTATGAGAA CCAGACCAAA 
CTGGCCCTCG TTGGGAACTG GGGCACAACA GGCCTTACCT ACCCCAAGTT TTCTGATGTC 
GCGGGCAAGA TCAAGCTCCC CAAGGATAGC TTCCGCCCCT CAGCCGGCTG GACCTGGGCT 
GGAGATTGGT TTGTGTGTCC AGAGAAGACG CTGCTCCATG ACACCGACGC GGGCCACCTG 
AGTTTCGTGG CGGAGGTGTT TGAGAACCAG ACCCGGCTCC CTGGAGGCCA CTGGGTCTAC 
ATGAGTGACA ACTACACGGA TGTGAATGGG GAGAAGGTGC TTCCCAAGGA TGACATTGAG 
TGCCCGCTGG GCTGGAAGTG GGAAGATGAA GAGTGGTCCA CGGACCTCAA TCGGGCCGTT 
GATGAGCAAG GCTGGGAGTA CAGTATCACC ATCCCCCCGG ACCGGAAGCC GAAGCACTGG 
GTCCCCGCTG AGAAGATGTA CTACACGCAC CGTCGGCGGC GCTGGGTCCG CCTGCGCAGG 
AGGGACCTCA GCCAGATGGA GGCCCTGAGG AAGCACAGGC AGGCGGAGGC GGAGGGCGAG 
GGCTGGGAGT ACGCCTCGCT CTTTGGCTGG AAATTCCACC TGGAGTACCG CAAAACGGAT 
GCATTCCGTC GCCGCCGCTG GCGCCGCCGC ATGGAGCCGC TGGAGAAGAC GGGGCCTGCA 
GCCGTGTTTG CCCTTGAGGG GGCACTGGGT GGCGTGGTAG ATGACCGGAG TGAAGATTCC 
ATGTCCGTGT CCACCTTGAG CTTTGGCGTG AACAGACCCA CGATTTCCTG CATCTTTGAT 
CATGGGAACC GCTACCACCT GCGCTGCTAT ATGTACCAGG CTCGGGACCT GCCCTCGATG 
GACAAGGACT CTTTTTCTGA TCCCTACGCC ATCGTCTCCT TCCTGCACCA GAGCCAGAAG 
ACCGTGGTGG TGAAGAACAC TCTGAACCCC ACCTGGGACC AGACGCTCAT CTTCTATGAG 
ATTGAGATCT TTGGAGAGCC AGCCAGCATC GCCGAGCAGC CGCCCAGCAT TGTGGTGGAG 
CTGTATGACC ACGACACCTA CGGTGCAGAT GAGTTTTTGG GACGCTGCAT GTGTCAGCCC 
AGTCTGGAAC GGATGCCCCG GCTGGCCTGG TTCCCGCTGG TTAGGGGCAG CCAGCCGGCA 
GGCGAGCTGC TGGCCTCTTT TGAGCTCATC CAGAGAGAGA AGCCGGCCAT TCACCATATT 
CCTGGTTTTG AGGTGCAGGA TACATCGGGA ATCCTGGACG AGCTGTGTGC ACCTGCCTTC 
TTCCTCCAGG GCCTCCTCCA AACGTCGGAG GACACAGACC TGCCCTACCC ACCGCCTCAG 
AGGGAGGCCA ACATCTACAT GGTTCCCCAG AACATCAAGC CGGTTCTCCA GCGCACTGCC 
ATAGAGATCC TGGCATGGGG CCTACGGAAC ATGAAGAGTT ACCAGCTGGC CAGTATCTCC 
TCCCCCAGCC TCGTGGTGGA GTGCGGGGGC CAGACGGTGC AGTCCTGTGT CATCAGGAAC 
CTCCGGAAGA ACCCCAACTT CGACATCTGC ACCCTCTTCA TGGAAGTGAT GCTGCCCAGA 
GAGGAGCTCT ACTGTCCCCC CATCGTGATC AAGGTCATTG ATAACCGCCA GTTTGGCCGC 
CGGCCTGTGG TGGGCCAGTG TACCATACGC TCCCTGGAGA GCTTCATGTG TGACCTGTAC 
TCCGAGGAGA GTCCGTCCCC ACAGGGCGGC CCAGATGATG TGAGTTTACT CAGTCCTGGG 
GAAGACGTGC TCATTGACAT CGACGACAAG GAGCCCCTCA TCCCCATACA GCTTACAGAC 
GGTCTGTTGG GCTCTGCCCC CACTAACATG GCATCCTCTC CATCCAGTCT TCATGAGGAG 
GAGTTCATCG ACTGGTGGAG CAAATTCTTT GCCTCCACAG GGGAGAGGGA GAAGTGCGGC 
TCGTACTTGG AGAAGGGCTT TGACACTCTA AAGGTCTATG ACACACAACT GGAGAACGTG 
GAGGCCTTTG GGGGCCTGTC TGACTTTTGT AACACCTTCA AGCTCTACCG GGGCAAGACT 
CAGGAGGAGA CGGAGGACCC ATCTGTTATC GGGGAGTTTA AGGGCCTCTT CAAAATCTAC 
CCCCTCCCGG AAGACCCGGC CGTCCCCATG CCCCCGAGAC AGTTCCAGCA GCTGGCTGCC 
CAGGGGCCTC AGGACTGCCT GGTCCGCATC TATATCATCC GGGCATTTGG CCTGCAGCCC 
AAGGACCCCA ATGGGAAGTG TGATCCTTAC ATCAAGATCT CCATAGGGAA GAAATCAGTG 
AGTGACCAGG ATAATTACAT TCCCTGTACC CTGGAGCCTG TGTTTGGAAA GATGTTCGAG 
CTGACCTGCA CTCTGCCTCT GGAGAAGGAC CTGAAGATCA CACTCTACGA CTATGACCTC 
CTCTCCAAAG ATGAGAAGAT CGGGGAGACA GTCATCGACC TGGAGAATAG GCTGCTGTCC 
AAGTTCGGGG CTCGCTGCGG ACTCCCACAG ACCTACTGTG TCTCTGGACC CAACCAGTGG 
AGGGACCAGC TCCGCCCCTC CCAGCTTCTC CAGCTCACCT GCCAGCAGCG CAGACTCAAG 
GCGCCCGTGT ACCTCACAGA CCGCGTACTC TTTCAGGATA AAGAATACAC CATTGAGGAA 
ATAGAGGCTG GCAGGGTCCT GAACCCACAC CTGGGCCCAG TGGAAGAGCG TCTGGCCTTG 
CACGTCCTCC AGCAGCTGGG CCTGGTCCCC GAGCATGTGG AGTCCCGGCC TCTTTACAGC 
CCCCTGCAGC CAGACATTGA GCAGGGGAAG CTGCAGATGT GGATCGATCT GTTTCCCAAG 
GCCCTGGGGC GACCTGGACC TCCCTTCAAC ATCACCCCAC GGAGAGCAAG AAGGTTTTTC 
CTGCGTTGTA TTATCTGGAA CACAAAGGAC GTGATCCTGG ACGACCTCAG CATCACGGGG 
GAGAAGATGA GTGACATTTA CGTGAAAGGT TGGATGGTCG GCTTTGAAGA GCACAAGCAG 
AAGACGGACG TGCACTACCG CTCCCTGGGA GGCGAAGGCA ACTTTAACTG GAGGTTCATT 
TTCCCCTTTG ACTACCTGCC CGCTGAGCAA GTCTGCACCG TCTCTAAGAA GGATGCTTTC 
TGGAGGCTGG ACAAGACTGA GAGCAAAATC CCAGCACAAG TGGTATTTCA GATCTGGGAC 
AATGACAAGT TCTCCTTTGA CGACTTTCTG GGCTCGTTGC AGTTGGATCT CAACCGCATG 
CCCAAGCCTG CCAAGACAGC TGAGAAGTGC TCCTTGGACC AGCTGGACGA CACTTTCCAC 
CCGGAGTGGT TTGTGTCCCT TTTTGAACAG AAAACAGTAA AGGGCTGGTG GCCCTGTGTG 
GCAGAAGACG GTGAGAAGAA GATCCTGGCG GGCAAGCTGG AAATGACCTT GGAGATCGTA 
GCAGAGAGTG AGCACGAAGA GCGGCCTGCG GGTCAAGGCC GGGATGAACC CAACATGAAC 
CCCAAGCTCG AGGACCCAAG GCGCCCCGAC ACCTCCTTCC TGTGGTTCAC CTCTCCGTAC 
AAGACCATGA AGTTCATCCT GTGGCGGCGG TTCCGGTGCG CCATCATCCT CTTCGTCGTT 
CTCGTCATCC TGCTGCTCTT CCTGGGCATC TTCCTCTATT CCTTCCCGAA CTATGCTGCC 
ATGAAGCTGG TGAAACCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019650380.1
CDS	349..6795
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEAQVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPSASLEPSPTLPDMDMVAGGGQSRAETWSLLSDSTVDTRYSGKKWLAPTDTGGEEDTEDQGLTGDEAEPLLDPSGAPGPGAPTTPRKPPSHPPPYHPGSKRKRGAPTPRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVMDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGVAKPSPAMDLDDHLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRLLLSLETKLVEHSDQKVEDLPADDILRVEKYLRRRKYSLFAAFYSASMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADRLEAALEQVHLAVKAQYNAEDVDSLLAQLMDELISDCSQPLGDVHETPSATHLDQYLHRLRSRHLSQIAEAALALKLGHGGLPAALEQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPTHEVLFSRRGASYCGKNCGKLQTIFLKYPMQGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVAGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDTDAGHLSFVAEVFENQTRLPGGHWVYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALRKHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSMSVSTLSFGVNRPTISCIFDHGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFLGRCMCQPSLERMPRLAWFPLVRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTSGILDELCAPAFFLQGLLQTSEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPIVIKVIDNRQFGRRPVVGQCTIRSLESFMCDLYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLTDGLLGSAPTNMASSPSSLHEEEFIDWWSKFFASTGEREKCGSYLEKGFDTLKVYDTQLENVEAFGGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAVPMPPRQFQQLAAQGPQDCLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLQLTCQQRRLKAPVYLTDRVLFQDKEYTIEEIEAGRVLNPHLGPVEERLALHVLQQLGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPAQVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEDGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFVVLVILLLFLGIFLYSFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_019794821.1
Organism	Ailuropoda melanoleuca(giant panda)
Definition	Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019794821.1

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCCGAG AATGTCCACA CCCCGGACAC CGACATCAGC 
GATGCCTACT GCTCTGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CAGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGAGCTCCG AGCTCCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
TTAGGGGAAG CCCAAGTCCC ACTCCGGGAG GTCCTCGCCA CCCCCAGTCT GTCTGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CGCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCCTCTGC ATCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATATG GTGGCTGGTG GGGGACAGAG CCGGGCTGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCTG 
GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAGGACC AGGGGCTCAC GGGAGATGAG 
GCAGAGCCAT TACTGGATCC GAGCGGTGCC CCAGGCCCTG GGGCCCCCAC CACCCCGAGG 
AAGCCACCTT CCCATCCGCC CCCCTACCAC CCCGGGAGCA AGAGAAAAAG AGGTGCTCCC 
ACGCCCAGAA AGCTGCTGTC GGACAAACCG CAGGACTTCC AGATCAGGGT GCAAGTGATC 
GAGGGGCGCC AGCTGCCAGG GGTGAATATC AAGCCTGTGG TCAAAGTCAC GGCTGCCGGG 
CAGACCAAGA GGACACGGAT CCACAAGGGA AACAGCCCAC TCTTCAACGA GACTCTCTTC 
TTCAATGTGT TTGACTCTCC CTCGGAGCTG TTTGACGAGC CCATCTTTAT CACGGTGGTA 
GACTCCCGTT CTCTCCGGAC AGACGCTCTC ATTGGGGAAT TCCGGATGGA CGTGGGTACC 
ATCTACAGAG AGCCCCGACA CGCCTATCTG AGGAAATGGC TTCTCCTCTC GGACCCTGAC 
GATTTCTCTG CTGGGGCCAG AGGTTACCTG AAAGCAAGCC TCTGTGTGCT GGGGCCCGGC 
GATGAAGCGC CTCTGGAGAG AAAGGACCCC TCTGAAGACA AGGAGGACAT CGAAAACAAT 
CTGCTCAGGC CAACTGGCGT GGCCCTTCGA GGAGCCCACT TCTGCCTGAA GGTCTTCAGA 
GCTGAGGACT TACCACAGAT GGATGATGCC GTGATGGACA ACGTGAAGCA GATCTTCGGC 
TTTGACAGTA ACAAGAAGAA CTTGGTGGAT CCCTTCATGG AAGTCAGCTT TGCGGGGAAA 
ATGCTCTGCA GCAAGATCCT GGAGAAAACA GCCAACCCTC AGTGGAACCA GAGCATCACG 
CTGCCCGCCA TGTTTCCCTC CATGTGTGAA AAAATGAGGA TTCGCGTCAT GGACTGGGAC 
CGCCTCACCC ACAATGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCCGCC 
CCTGGAGGAG AAATAGAAGA GGAGCCTGCA GGTGTGGCCA AGCCTTCTCC AGCCATGGAC 
CTGGACGACC ATCTGGGCTT CCTCCCCACC TTCGGGCCCT GCTACGTCAA CCTCTACGGC 
AGCCCCAGGG AGTTCACCGG CTTCCCGGAC CCCTACGCAG AACTCAACAT GGGCAAGGGA 
GAAGGTGTGG CCTACCGAGG TCGGCTTCTG CTCTCCCTGG AGACCAAGCT GGTGGAGCAC 
AGCGACCAAA AGGTGGAGGA CCTCCCTGCG GATGACATCC TTCGGGTGGA GAAGTATCTC 
CGGAGGCGTA AGTACTCCCT CTTTGCCGCC TTCTACTCGG CCTCCATGCT CCAGGATGTG 
GATGATGCCA TCCAGTTCGA GGTCAGCATC GGGAACTATG GCAACAAGTT CGACACCACG 
TGCCTGCCGC TGGCCTCTAC CACCCAGTAC AGCCGGGCGG TCTTTGACGG GTGCCACTAC 
TACTACTTGC CCTGGGGCAA CGTGAAGCCC GTGGTGGTGC TGTCATCCTA CTGGGAGGAC 
ATCAGCCACA GAGTTGAGGC TCAGAACCAG CTGCTCAGGA TTGCGGACCG GCTGGAAGCT 
GCCCTGGAGC AAGTCCACTT GGCCGTGAAG GCGCAGTACA ACGCCGAGGA CGTGGACTCC 
CTGTTGGCTC AGCTGATGGA TGAGCTCATC TCAGACTGCA GCCAGCCCCT AGGCGATGTC 
CACGAGACAC CCTCTGCCAC CCACCTGGAC CAGTACCTGC ACAGGCTGCG TAGCCGTCAC 
CTAAGCCAGA TTGCAGAGGC GGCGCTGGCC CTGAAGCTCG GGCACGGCGG GCTCCCGGCC 
GCCCTGGAGC AGGCTGAAGA CTGGCTCCTG CGGCTCCGTG CCCTGGCCGA GGAGCCTCAG 
AACAGCCTGC CCGACATCAT CATCTGGATG CTGCAGGGCG ACAAGCGAGT GGCATACCAG 
CGGGTGCCTA CACATGAGGT CCTCTTCTCC CGGCGTGGAG CCAGCTACTG CGGCAAGAAC 
TGTGGGAAGC TGCAGACGAT ATTTCTCAAA TATCCCATGC AGGGGGTGCC CGGAGCCCGG 
ATGCCCGTGC AGATACGGGT GAAGCTCTGG TTTGGGCTTT CTGTGGATGA GAAGGAGTTC 
AACCAGTTTG CTGAGGGAAA GCTGTCTGTC TTTGCTGAAA CCTATGAGAA CCAGACCAAA 
CTGGCCCTCG TTGGGAACTG GGGCACAACA GGCCTTACCT ACCCCAAGTT TTCTGATGTC 
GCGGGCAAGA TCAAGCTCCC CAAGGATAGC TTCCGCCCCT CAGCCGGCTG GACCTGGGCT 
GGAGATTGGT TTGTGTGTCC AGAGAAGACG CTGCTCCATG ACACCGACGC GGGCCACCTG 
AGTTTCGTGG CGGAGGTGTT TGAGAACCAG ACCCGGCTCC CTGGAGGCCA CTGGGTCTAC 
ATGAGTGACA ACTACACGGA TGTGAATGGG GAGAAGGTGC TTCCCAAGGA TGACATTGAG 
TGCCCGCTGG GCTGGAAGTG GGAAGATGAA GAGTGGTCCA CGGACCTCAA TCGGGCCGTT 
GATGAGCAAG GCTGGGAGTA CAGTATCACC ATCCCCCCGG ACCGGAAGCC GAAGCACTGG 
GTCCCCGCTG AGAAGATGTA CTACACGCAC CGTCGGCGGC GCTGGGTCCG CCTGCGCAGG 
AGGGACCTCA GCCAGATGGA GGCCCTGAGG AAGCACAGGC AGGCGGAGGC GGAGGGCGAG 
GGCTGGGAGT ACGCCTCGCT CTTTGGCTGG AAATTCCACC TGGAGTACCG CAAAACGGAT 
GCATTCCGTC GCCGCCGCTG GCGCCGCCGC ATGGAGCCGC TGGAGAAGAC GGGGCCTGCA 
GCCGTGTTTG CCCTTGAGGG GGCACTGGGT GGCGTGGTAG ATGACCGGAG TGAAGATTCC 
ATGTCCGTGT CCACCTTGAG CTTTGGCGTG AACAGACCCA CGATTTCCTG CATCTTTGAT 
CATGGGAACC GCTACCACCT GCGCTGCTAT ATGTACCAGG CTCGGGACCT GCCCTCGATG 
GACAAGGACT CTTTTTCTGA TCCCTACGCC ATCGTCTCCT TCCTGCACCA GAGCCAGAAG 
ACCGTGGTGG TGAAGAACAC TCTGAACCCC ACCTGGGACC AGACGCTCAT CTTCTATGAG 
ATTGAGATCT TTGGAGAGCC AGCCAGCATC GCCGAGCAGC CGCCCAGCAT TGTGGTGGAG 
CTGTATGACC ACGACACCTA CGGTGCAGAT GAGTTTTTGG GACGCTGCAT GTGTCAGCCC 
AGTCTGGAAC GGATGCCCCG GCTGGCCTGG TTCCCGCTGG TTAGGGGCAG CCAGCCGGCA 
GGCGAGCTGC TGGCCTCTTT TGAGCTCATC CAGAGAGAGA AGCCGGCCAT TCACCATATT 
CCTGGTTTTG AGGTGCAGGA TACATCGGGA ATCCTGGACG AGCTGTGTGC ACCTGCCTTC 
TTCCTCCAGG GCCTCCTCCA AACGTCGGAG GACACAGACC TGCCCTACCC ACCGCCTCAG 
AGGGAGGCCA ACATCTACAT GGTTCCCCAG AACATCAAGC CGGTTCTCCA GCGCACTGCC 
ATAGAGATCC TGGCATGGGG CCTACGGAAC ATGAAGAGTT ACCAGCTGGC CAGTATCTCC 
TCCCCCAGCC TCGTGGTGGA GTGCGGGGGC CAGACGGTGC AGTCCTGTGT CATCAGGAAC 
CTCCGGAAGA ACCCCAACTT CGACATCTGC ACCCTCTTCA TGGAAGTGAT GCTGCCCAGA 
GAGGAGCTCT ACTGTCCCCC CATCGTGATC AAGGTCATTG ATAACCGCCA GTTTGGCCGC 
CGGCCTGTGG TGGGCCAGTG TACCATACGC TCCCTGGAGA GCTTCATGTG TGACCTGTAC 
TCCGAGGAGA GTCCGTCCCC ACAGGGCGGC CCAGATGATG TGAGTTTACT CAGTCCTGGG 
GAAGACGTGC TCATTGACAT CGACGACAAG GAGCCCCTCA TCCCCATACA GCTTACAGAC 
GGTCTGTTGG GCTCTGCCCC CACTAACATG GCATCCTCTC CATCCAGTCT TCATGAGGAG 
GAGTTCATCG ACTGGTGGAG CAAATTCTTT GCCTCCACAG GGGAGAGGGA GAAGTGCGGC 
TCGTACTTGG AGAAGGGCTT TGACACTCTA AAGGTCTATG ACACACAACT GGAGAACGTG 
GAGGCCTTTG GGGGCCTGTC TGACTTTTGT AACACCTTCA AGCTCTACCG GGGCAAGACT 
CAGGAGGAGA CGGAGGACCC ATCTGTTATC GGGGAGTTTA AGGGCCTCTT CAAAATCTAC 
CCCCTCCCGG AAGACCCGGC CGTCCCCATG CCCCCGAGAC AGTTCCAGCA GCTGGCTGCC 
CAGGGGCCTC AGGACTGCCT GGTCCGCATC TATATCATCC GGGCATTTGG CCTGCAGCCC 
AAGGACCCCA ATGGGAAGTG TGATCCTTAC ATCAAGATCT CCATAGGGAA GAAATCAGTG 
AGTGACCAGG ATAATTACAT TCCCTGTACC CTGGAGCCTG TGTTTGGAAA GATGTTCGAG 
CTGACCTGCA CTCTGCCTCT GGAGAAGGAC CTGAAGATCA CACTCTACGA CTATGACCTC 
CTCTCCAAAG ATGAGAAGAT CGGGGAGACA GTCATCGACC TGGAGAATAG GCTGCTGTCC 
AAGTTCGGGG CTCGCTGCGG ACTCCCACAG ACCTACTGTG TCTCTGGACC CAACCAGTGG 
AGGGACCAGC TCCGCCCCTC CCAGCTTCTC CAGCTCACCT GCCAGCAGCG CAGACTCAAG 
GCGCCCGTGT ACCTCACAGA CCGCGTACTC TTTCAGGATA AAGAATACAC CATTGAGGAA 
ATAGAGGCTG GCAGGGTCCT GAACCCACAC CTGGGCCCAG TGGAAGAGCG TCTGGCCTTG 
CACGTCCTCC AGCAGCTGGG CCTGGTCCCC GAGCATGTGG AGTCCCGGCC TCTTTACAGC 
CCCCTGCAGC CAGACATTGA GCAGGGGAAG CTGCAGATGT GGATCGATCT GTTTCCCAAG 
GCCCTGGGGC GACCTGGACC TCCCTTCAAC ATCACCCCAC GGAGAGCAAG AAGGTTTTTC 
CTGCGTTGTA TTATCTGGAA CACAAAGGAC GTGATCCTGG ACGACCTCAG CATCACGGGG 
GAGAAGATGA GTGACATTTA CGTGAAAGGT TGGATGGTCG GCTTTGAAGA GCACAAGCAG 
AAGACGGACG TGCACTACCG CTCCCTGGGA GGCGAAGGCA ACTTTAACTG GAGGTTCATT 
TTCCCCTTTG ACTACCTGCC CGCTGAGCAA GTCTGCACCG TCTCTAAGAA GGATGCTTTC 
TGGAGGCTGG ACAAGACTGA GAGCAAAATC CCAGCACAAG TGGTATTTCA GATCTGGGAC 
AATGACAAGT TCTCCTTTGA CGACTTTCTG GGCTCGTTGC AGTTGGATCT CAACCGCATG 
CCCAAGCCTG CCAAGACAGC TGAGAAGTGC TCCTTGGACC AGCTGGACGA CACTTTCCAC 
CCGGAGTGGT TTGTGTCCCT TTTTGAACAG AAAACAGTAA AGGGCTGGTG GCCCTGTGTG 
GCAGAAGACG GTGAGAAGAA GATCCTGGCG GGCAAGCTGG AAATGACCTT GGAGATCGTA 
GCAGAGAGTG AGCACGAAGA GCGGCCTGCG GGTCAAGGCC GGGATGAACC CAACATGAAC 
CCCAAGCTCG AGGACCCAAG GCGCCCCGAC ACCTCCTTCC TGTGGTTCAC CTCTCCGTAC 
AAGACCATGA AGTTCATCCT GTGGCGGCGG TTCCGGTGCG CCATCATCCT CTTCGTCGTT 
CTCGTCATCC TGCTGCTCTT CCTGGGCATC TTCCTCTATT CCTTCCCGAA CTATGCTGCC 
ATGAAGCTGG TGAAACCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_019794822.1 ) cDNA ORF clone, Ailuropoda melanoleuca(giant panda) -> XP_019650381.1 Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X3, mRNA.

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CloneID	OAc89676
Clone ID Related Accession (Same CDS sequence)	XM_019794822.1
Accession Version	XM_019794822.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-27
Organism	Ailuropoda melanoleuca(giant panda)
Product	dysferlin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003217356.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ailuropoda melanoleuca Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGTGAAGAA GGACCGGCGC 
AGTGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCAGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTCCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCGG GAGGTCCTCG CCACCCCCAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCCTC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGTGGCTG GTGGGGGACA GAGCCGGGCT 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACGGGAGAT 
GAGGCAGAGC CATTACTGGA TCCGAGCGGT GCCCCAGGCC CTGGGGCCCC CACCACCCCG 
AGGAAGCCAC CTTCCCATCC GCCCCCCTAC CACCCCGGGA GCAAGAGAAA AAGAGGTGCT 
CCCACGCCCA GAAAGCTGCT GTCGGACAAA CCGCAGGACT TCCAGATCAG GGTGCAAGTG 
ATCGAGGGGC GCCAGCTGCC AGGGGTGAAT ATCAAGCCTG TGGTCAAAGT CACGGCTGCC 
GGGCAGACCA AGAGGACACG GATCCACAAG GGAAACAGCC CACTCTTCAA CGAGACTCTC 
TTCTTCAATG TGTTTGACTC TCCCTCGGAG CTGTTTGACG AGCCCATCTT TATCACGGTG 
GTAGACTCCC GTTCTCTCCG GACAGACGCT CTCATTGGGG AATTCCGGAT GGACGTGGGT 
ACCATCTACA GAGAGCCCCG ACACGCCTAT CTGAGGAAAT GGCTTCTCCT CTCGGACCCT 
GACGATTTCT CTGCTGGGGC CAGAGGTTAC CTGAAAGCAA GCCTCTGTGT GCTGGGGCCC 
GGCGATGAAG CGCCTCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATCGAAAAC 
AATCTGCTCA GGCCAACTGG CGTGGCCCTT CGAGGAGCCC ACTTCTGCCT GAAGGTCTTC 
AGAGCTGAGG ACTTACCACA GATGGATGAT GCCGTGATGG ACAACGTGAA GCAGATCTTC 
GGCTTTGACA GTAACAAGAA GAACTTGGTG GATCCCTTCA TGGAAGTCAG CTTTGCGGGG 
AAAATGCTCT GCAGCAAGAT CCTGGAGAAA ACAGCCAACC CTCAGTGGAA CCAGAGCATC 
ACGCTGCCCG CCATGTTTCC CTCCATGTGT GAAAAAATGA GGATTCGCGT CATGGACTGG 
GACCGCCTCA CCCACAATGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCC 
GCCCCTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTGG CCAAGCCTTC TCCAGCCATG 
GACCTGGACG ACCATCTGGG CTTCCTCCCC ACCTTCGGGC CCTGCTACGT CAACCTCTAC 
GGCAGCCCCA GGGAGTTCAC CGGCTTCCCG GACCCCTACG CAGAACTCAA CATGGGCAAG 
GGAGAAGGTG TGGCCTACCG AGGTCGGCTT CTGCTCTCCC TGGAGACCAA GCTGGTGGAG 
CACAGCGACC AAAAGGTGGA GGACCTCCCT GCGGATGACA TCCTTCGGGT GGAGAAGTAT 
CTCCGGAGGC GTAAGTACTC CCTCTTTGCC GCCTTCTACT CGGCCTCCAT GCTCCAGGAT 
GTGGATGATG CCATCCAGTT CGAGGTCAGC ATCGGGAACT ATGGCAACAA GTTCGACACC 
ACGTGCCTGC CGCTGGCCTC TACCACCCAG TACAGCCGGG CGGTCTTTGA CGGGTGCCAC 
TACTACTACT TGCCCTGGGG CAACGTGAAG CCCGTGGTGG TGCTGTCATC CTACTGGGAG 
GACATCAGCC ACAGAGTTGA GGCTCAGAAC CAGCTGCTCA GGATTGCGGA CCGGCTGGAA 
GCTGCCCTGG AGCAAGTCCA CTTGGCCGTG AAGGCGCAGT ACAACGCCGA GGACGTGGAC 
TCCCTGTTGG CTCAGCTGAT GGATGAGCTC ATCTCAGACT GCAGCCAGCC CCTAGGCGAT 
GTCCACGAGA CACCCTCTGC CACCCACCTG GACCAGTACC TGCACAGGCT GCGTAGCCGT 
CACCTAAGCC AGATTGCAGA GGCGGCGCTG GCCCTGAAGC TCGGGCACGG CGGGCTCCCG 
GCCGCCCTGG AGCAGGCTGA AGACTGGCTC CTGCGGCTCC GTGCCCTGGC CGAGGAGCCT 
CAGAACAGCC TGCCCGACAT CATCATCTGG ATGCTGCAGG GCGACAAGCG AGTGGCATAC 
CAGCGGGTGC CTACACATGA GGTCCTCTTC TCCCGGCGTG GAGCCAGCTA CTGCGGCAAG 
AACTGTGGGA AGCTGCAGAC GATATTTCTC AAATATCCCA TGCAGGGGGT GCCCGGAGCC 
CGGATGCCCG TGCAGATACG GGTGAAGCTC TGGTTTGGGC TTTCTGTGGA TGAGAAGGAG 
TTCAACCAGT TTGCTGAGGG AAAGCTGTCT GTCTTTGCTG AAACCTATGA GAACCAGACC 
AAACTGGCCC TCGTTGGGAA CTGGGGCACA ACAGGCCTTA CCTACCCCAA GTTTTCTGAT 
GTCGCGGGCA AGATCAAGCT CCCCAAGGAT AGCTTCCGCC CCTCAGCCGG CTGGACCTGG 
GCTGGAGATT GGTTTGTGTG TCCAGAGAAG ACGCTGCTCC ATGACACCGA CGCGGGCCAC 
CTGAGTTTCG TGGCGGAGGT GTTTGAGAAC CAGACCCGGC TCCCTGGAGG CCACTGGGTC 
TACATGAGTG ACAACTACAC GGATGTGAAT GGGGAGAAGG TGCTTCCCAA GGATGACATT 
GAGTGCCCGC TGGGCTGGAA GTGGGAAGAT GAAGAGTGGT CCACGGACCT CAATCGGGCC 
GTTGATGAGC AAGGCTGGGA GTACAGTATC ACCATCCCCC CGGACCGGAA GCCGAAGCAC 
TGGGTCCCCG CTGAGAAGAT GTACTACACG CACCGTCGGC GGCGCTGGGT CCGCCTGCGC 
AGGAGGGACC TCAGCCAGAT GGAGGCCCTG AGGAAGCACA GGCAGGCGGA GGCGGAGGGC 
GAGGGCTGGG AGTACGCCTC GCTCTTTGGC TGGAAATTCC ACCTGGAGTA CCGCAAAACG 
GATGCATTCC GTCGCCGCCG CTGGCGCCGC CGCATGGAGC CGCTGGAGAA GACGGGGCCT 
GCAGCCGTGT TTGCCCTTGA GGGGGCACTG GGTGGCGTGG TAGATGACCG GAGTGAAGAT 
TCCATGTCCG TGTCCACCTT GAGCTTTGGC GTGAACAGAC CCACGATTTC CTGCATCTTT 
GATCATGGGA ACCGCTACCA CCTGCGCTGC TATATGTACC AGGCTCGGGA CCTGCCCTCG 
ATGGACAAGG ACTCTTTTTC TGATCCCTAC GCCATCGTCT CCTTCCTGCA CCAGAGCCAG 
AAGACCGTGG TGGTGAAGAA CACTCTGAAC CCCACCTGGG ACCAGACGCT CATCTTCTAT 
GAGATTGAGA TCTTTGGAGA GCCAGCCAGC ATCGCCGAGC AGCCGCCCAG CATTGTGGTG 
GAGCTGTATG ACCACGACAC CTACGGTGCA GATGAGTTTT TGGGACGCTG CATGTGTCAG 
CCCAGTCTGG AACGGATGCC CCGGCTGGCC TGGTTCCCGC TGGTTAGGGG CAGCCAGCCG 
GCAGGCGAGC TGCTGGCCTC TTTTGAGCTC ATCCAGAGAG AGAAGCCGGC CATTCACCAT 
ATTCCTGGTT TTGAGGTGCA GGATACATCG GGAATCCTGG ACGAGTCGGA GGACACAGAC 
CTGCCCTACC CACCGCCTCA GAGGGAGGCC AACATCTACA TGGTTCCCCA GAACATCAAG 
CCGGTTCTCC AGCGCACTGC CATAGAGATC CTGGCATGGG GCCTACGGAA CATGAAGAGT 
TACCAGCTGG CCAGTATCTC CTCCCCCAGC CTCGTGGTGG AGTGCGGGGG CCAGACGGTG 
CAGTCCTGTG TCATCAGGAA CCTCCGGAAG AACCCCAACT TCGACATCTG CACCCTCTTC 
ATGGAAGTGA TGCTGCCCAG AGAGGAGCTC TACTGTCCCC CCATCGTGAT CAAGGTCATT 
GATAACCGCC AGTTTGGCCG CCGGCCTGTG GTGGGCCAGT GTACCATACG CTCCCTGGAG 
AGCTTCATGT GTGACCTGTA CTCCGAGGAG AGTCCGTCCC CACAGGGCGG CCCAGATGAT 
GTGAGTTTAC TCAGTCCTGG GGAAGACGTG CTCATTGACA TCGACGACAA GGAGCCCCTC 
ATCCCCATAC AGCTTACAGA CGGTCTGTTG GGCTCTGCCC CCACTAACAT GGCATCCTCT 
CCATCCAGTC TTCATGAGGA GGAGTTCATC GACTGGTGGA GCAAATTCTT TGCCTCCACA 
GGGGAGAGGG AGAAGTGCGG CTCGTACTTG GAGAAGGGCT TTGACACTCT AAAGGTCTAT 
GACACACAAC TGGAGAACGT GGAGGCCTTT GGGGGCCTGT CTGACTTTTG TAACACCTTC 
AAGCTCTACC GGGGCAAGAC TCAGGAGGAG ACGGAGGACC CATCTGTTAT CGGGGAGTTT 
AAGGGCCTCT TCAAAATCTA CCCCCTCCCG GAAGACCCGG CCGTCCCCAT GCCCCCGAGA 
CAGTTCCAGC AGCTGGCTGC CCAGGGGCCT CAGGACTGCC TGGTCCGCAT CTATATCATC 
CGGGCATTTG GCCTGCAGCC CAAGGACCCC AATGGGAAGT GTGATCCTTA CATCAAGATC 
TCCATAGGGA AGAAATCAGT GAGTGACCAG GATAATTACA TTCCCTGTAC CCTGGAGCCT 
GTGTTTGGAA AGATGTTCGA GCTGACCTGC ACTCTGCCTC TGGAGAAGGA CCTGAAGATC 
ACACTCTACG ACTATGACCT CCTCTCCAAA GATGAGAAGA TCGGGGAGAC AGTCATCGAC 
CTGGAGAATA GGCTGCTGTC CAAGTTCGGG GCTCGCTGCG GACTCCCACA GACCTACTGT 
GTCTCTGGAC CCAACCAGTG GAGGGACCAG CTCCGCCCCT CCCAGCTTCT CCAGCTCACC 
TGCCAGCAGC GCAGACTCAA GGCGCCCGTG TACCTCACAG ACCGCGTACT CTTTCAGGAT 
AAAGAATACA CCATTGAGGA AATAGAGGCT GGCAGGGTCC TGAACCCACA CCTGGGCCCA 
GTGGAAGAGC GTCTGGCCTT GCACGTCCTC CAGCAGCTGG GCCTGGTCCC CGAGCATGTG 
GAGTCCCGGC CTCTTTACAG CCCCCTGCAG CCAGACATTG AGCAGGGGAA GCTGCAGATG 
TGGATCGATC TGTTTCCCAA GGCCCTGGGG CGACCTGGAC CTCCCTTCAA CATCACCCCA 
CGGAGAGCAA GAAGGTTTTT CCTGCGTTGT ATTATCTGGA ACACAAAGGA CGTGATCCTG 
GACGACCTCA GCATCACGGG GGAGAAGATG AGTGACATTT ACGTGAAAGG TTGGATGGTC 
GGCTTTGAAG AGCACAAGCA GAAGACGGAC GTGCACTACC GCTCCCTGGG AGGCGAAGGC 
AACTTTAACT GGAGGTTCAT TTTCCCCTTT GACTACCTGC CCGCTGAGCA AGTCTGCACC 
GTCTCTAAGA AGGATGCTTT CTGGAGGCTG GACAAGACTG AGAGCAAAAT CCCAGCACAA 
GTGGTATTTC AGATCTGGGA CAATGACAAG TTCTCCTTTG ACGACTTTCT GGGCTCGTTG 
CAGTTGGATC TCAACCGCAT GCCCAAGCCT GCCAAGACAG CTGAGAAGTG CTCCTTGGAC 
CAGCTGGACG ACACTTTCCA CCCGGAGTGG TTTGTGTCCC TTTTTGAACA GAAAACAGTA 
AAGGGCTGGT GGCCCTGTGT GGCAGAAGAC GGTGAGAAGA AGATCCTGGC GGGCAAGCTG 
GAAATGACCT TGGAGATCGT AGCAGAGAGT GAGCACGAAG AGCGGCCTGC GGGTCAAGGC 
CGGGATGAAC CCAACATGAA CCCCAAGCTC GAGGACCCAA GGCGCCCCGA CACCTCCTTC 
CTGTGGTTCA CCTCTCCGTA CAAGACCATG AAGTTCATCC TGTGGCGGCG GTTCCGGTGC 
GCCATCATCC TCTTCGTCGT TCTCGTCATC CTGCTGCTCT TCCTGGGCAT CTTCCTCTAT 
TCCTTCCCGA ACTATGCTGC CATGAAGCTG GTGAAACCCT TCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019650381.1
CDS	150..6557
Translation	MLSCLLVRASNLPSVKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEAQVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPSASLEPSPTLPDMDMVAGGGQSRAETWSLLSDSTVDTRYSGKKWLAPTDTGGEEDTEDQGLTGDEAEPLLDPSGAPGPGAPTTPRKPPSHPPPYHPGSKRKRGAPTPRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVMDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGVAKPSPAMDLDDHLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRLLLSLETKLVEHSDQKVEDLPADDILRVEKYLRRRKYSLFAAFYSASMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADRLEAALEQVHLAVKAQYNAEDVDSLLAQLMDELISDCSQPLGDVHETPSATHLDQYLHRLRSRHLSQIAEAALALKLGHGGLPAALEQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPTHEVLFSRRGASYCGKNCGKLQTIFLKYPMQGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVAGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDTDAGHLSFVAEVFENQTRLPGGHWVYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALRKHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSMSVSTLSFGVNRPTISCIFDHGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFLGRCMCQPSLERMPRLAWFPLVRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTSGILDESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPIVIKVIDNRQFGRRPVVGQCTIRSLESFMCDLYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLTDGLLGSAPTNMASSPSSLHEEEFIDWWSKFFASTGEREKCGSYLEKGFDTLKVYDTQLENVEAFGGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAVPMPPRQFQQLAAQGPQDCLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLQLTCQQRRLKAPVYLTDRVLFQDKEYTIEEIEAGRVLNPHLGPVEERLALHVLQQLGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPAQVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEDGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFVVLVILLLFLGIFLYSFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_019794822.1
Organism	Ailuropoda melanoleuca(giant panda)
Definition	Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019794822.1

ORF Insert Sequence:

ATGCTGAGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGTGAAGAA GGACCGGCGC 
AGTGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCAGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTCCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCGG GAGGTCCTCG CCACCCCCAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCCTC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGTGGCTG GTGGGGGACA GAGCCGGGCT 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACGGGAGAT 
GAGGCAGAGC CATTACTGGA TCCGAGCGGT GCCCCAGGCC CTGGGGCCCC CACCACCCCG 
AGGAAGCCAC CTTCCCATCC GCCCCCCTAC CACCCCGGGA GCAAGAGAAA AAGAGGTGCT 
CCCACGCCCA GAAAGCTGCT GTCGGACAAA CCGCAGGACT TCCAGATCAG GGTGCAAGTG 
ATCGAGGGGC GCCAGCTGCC AGGGGTGAAT ATCAAGCCTG TGGTCAAAGT CACGGCTGCC 
GGGCAGACCA AGAGGACACG GATCCACAAG GGAAACAGCC CACTCTTCAA CGAGACTCTC 
TTCTTCAATG TGTTTGACTC TCCCTCGGAG CTGTTTGACG AGCCCATCTT TATCACGGTG 
GTAGACTCCC GTTCTCTCCG GACAGACGCT CTCATTGGGG AATTCCGGAT GGACGTGGGT 
ACCATCTACA GAGAGCCCCG ACACGCCTAT CTGAGGAAAT GGCTTCTCCT CTCGGACCCT 
GACGATTTCT CTGCTGGGGC CAGAGGTTAC CTGAAAGCAA GCCTCTGTGT GCTGGGGCCC 
GGCGATGAAG CGCCTCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATCGAAAAC 
AATCTGCTCA GGCCAACTGG CGTGGCCCTT CGAGGAGCCC ACTTCTGCCT GAAGGTCTTC 
AGAGCTGAGG ACTTACCACA GATGGATGAT GCCGTGATGG ACAACGTGAA GCAGATCTTC 
GGCTTTGACA GTAACAAGAA GAACTTGGTG GATCCCTTCA TGGAAGTCAG CTTTGCGGGG 
AAAATGCTCT GCAGCAAGAT CCTGGAGAAA ACAGCCAACC CTCAGTGGAA CCAGAGCATC 
ACGCTGCCCG CCATGTTTCC CTCCATGTGT GAAAAAATGA GGATTCGCGT CATGGACTGG 
GACCGCCTCA CCCACAATGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCC 
GCCCCTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTGG CCAAGCCTTC TCCAGCCATG 
GACCTGGACG ACCATCTGGG CTTCCTCCCC ACCTTCGGGC CCTGCTACGT CAACCTCTAC 
GGCAGCCCCA GGGAGTTCAC CGGCTTCCCG GACCCCTACG CAGAACTCAA CATGGGCAAG 
GGAGAAGGTG TGGCCTACCG AGGTCGGCTT CTGCTCTCCC TGGAGACCAA GCTGGTGGAG 
CACAGCGACC AAAAGGTGGA GGACCTCCCT GCGGATGACA TCCTTCGGGT GGAGAAGTAT 
CTCCGGAGGC GTAAGTACTC CCTCTTTGCC GCCTTCTACT CGGCCTCCAT GCTCCAGGAT 
GTGGATGATG CCATCCAGTT CGAGGTCAGC ATCGGGAACT ATGGCAACAA GTTCGACACC 
ACGTGCCTGC CGCTGGCCTC TACCACCCAG TACAGCCGGG CGGTCTTTGA CGGGTGCCAC 
TACTACTACT TGCCCTGGGG CAACGTGAAG CCCGTGGTGG TGCTGTCATC CTACTGGGAG 
GACATCAGCC ACAGAGTTGA GGCTCAGAAC CAGCTGCTCA GGATTGCGGA CCGGCTGGAA 
GCTGCCCTGG AGCAAGTCCA CTTGGCCGTG AAGGCGCAGT ACAACGCCGA GGACGTGGAC 
TCCCTGTTGG CTCAGCTGAT GGATGAGCTC ATCTCAGACT GCAGCCAGCC CCTAGGCGAT 
GTCCACGAGA CACCCTCTGC CACCCACCTG GACCAGTACC TGCACAGGCT GCGTAGCCGT 
CACCTAAGCC AGATTGCAGA GGCGGCGCTG GCCCTGAAGC TCGGGCACGG CGGGCTCCCG 
GCCGCCCTGG AGCAGGCTGA AGACTGGCTC CTGCGGCTCC GTGCCCTGGC CGAGGAGCCT 
CAGAACAGCC TGCCCGACAT CATCATCTGG ATGCTGCAGG GCGACAAGCG AGTGGCATAC 
CAGCGGGTGC CTACACATGA GGTCCTCTTC TCCCGGCGTG GAGCCAGCTA CTGCGGCAAG 
AACTGTGGGA AGCTGCAGAC GATATTTCTC AAATATCCCA TGCAGGGGGT GCCCGGAGCC 
CGGATGCCCG TGCAGATACG GGTGAAGCTC TGGTTTGGGC TTTCTGTGGA TGAGAAGGAG 
TTCAACCAGT TTGCTGAGGG AAAGCTGTCT GTCTTTGCTG AAACCTATGA GAACCAGACC 
AAACTGGCCC TCGTTGGGAA CTGGGGCACA ACAGGCCTTA CCTACCCCAA GTTTTCTGAT 
GTCGCGGGCA AGATCAAGCT CCCCAAGGAT AGCTTCCGCC CCTCAGCCGG CTGGACCTGG 
GCTGGAGATT GGTTTGTGTG TCCAGAGAAG ACGCTGCTCC ATGACACCGA CGCGGGCCAC 
CTGAGTTTCG TGGCGGAGGT GTTTGAGAAC CAGACCCGGC TCCCTGGAGG CCACTGGGTC 
TACATGAGTG ACAACTACAC GGATGTGAAT GGGGAGAAGG TGCTTCCCAA GGATGACATT 
GAGTGCCCGC TGGGCTGGAA GTGGGAAGAT GAAGAGTGGT CCACGGACCT CAATCGGGCC 
GTTGATGAGC AAGGCTGGGA GTACAGTATC ACCATCCCCC CGGACCGGAA GCCGAAGCAC 
TGGGTCCCCG CTGAGAAGAT GTACTACACG CACCGTCGGC GGCGCTGGGT CCGCCTGCGC 
AGGAGGGACC TCAGCCAGAT GGAGGCCCTG AGGAAGCACA GGCAGGCGGA GGCGGAGGGC 
GAGGGCTGGG AGTACGCCTC GCTCTTTGGC TGGAAATTCC ACCTGGAGTA CCGCAAAACG 
GATGCATTCC GTCGCCGCCG CTGGCGCCGC CGCATGGAGC CGCTGGAGAA GACGGGGCCT 
GCAGCCGTGT TTGCCCTTGA GGGGGCACTG GGTGGCGTGG TAGATGACCG GAGTGAAGAT 
TCCATGTCCG TGTCCACCTT GAGCTTTGGC GTGAACAGAC CCACGATTTC CTGCATCTTT 
GATCATGGGA ACCGCTACCA CCTGCGCTGC TATATGTACC AGGCTCGGGA CCTGCCCTCG 
ATGGACAAGG ACTCTTTTTC TGATCCCTAC GCCATCGTCT CCTTCCTGCA CCAGAGCCAG 
AAGACCGTGG TGGTGAAGAA CACTCTGAAC CCCACCTGGG ACCAGACGCT CATCTTCTAT 
GAGATTGAGA TCTTTGGAGA GCCAGCCAGC ATCGCCGAGC AGCCGCCCAG CATTGTGGTG 
GAGCTGTATG ACCACGACAC CTACGGTGCA GATGAGTTTT TGGGACGCTG CATGTGTCAG 
CCCAGTCTGG AACGGATGCC CCGGCTGGCC TGGTTCCCGC TGGTTAGGGG CAGCCAGCCG 
GCAGGCGAGC TGCTGGCCTC TTTTGAGCTC ATCCAGAGAG AGAAGCCGGC CATTCACCAT 
ATTCCTGGTT TTGAGGTGCA GGATACATCG GGAATCCTGG ACGAGTCGGA GGACACAGAC 
CTGCCCTACC CACCGCCTCA GAGGGAGGCC AACATCTACA TGGTTCCCCA GAACATCAAG 
CCGGTTCTCC AGCGCACTGC CATAGAGATC CTGGCATGGG GCCTACGGAA CATGAAGAGT 
TACCAGCTGG CCAGTATCTC CTCCCCCAGC CTCGTGGTGG AGTGCGGGGG CCAGACGGTG 
CAGTCCTGTG TCATCAGGAA CCTCCGGAAG AACCCCAACT TCGACATCTG CACCCTCTTC 
ATGGAAGTGA TGCTGCCCAG AGAGGAGCTC TACTGTCCCC CCATCGTGAT CAAGGTCATT 
GATAACCGCC AGTTTGGCCG CCGGCCTGTG GTGGGCCAGT GTACCATACG CTCCCTGGAG 
AGCTTCATGT GTGACCTGTA CTCCGAGGAG AGTCCGTCCC CACAGGGCGG CCCAGATGAT 
GTGAGTTTAC TCAGTCCTGG GGAAGACGTG CTCATTGACA TCGACGACAA GGAGCCCCTC 
ATCCCCATAC AGCTTACAGA CGGTCTGTTG GGCTCTGCCC CCACTAACAT GGCATCCTCT 
CCATCCAGTC TTCATGAGGA GGAGTTCATC GACTGGTGGA GCAAATTCTT TGCCTCCACA 
GGGGAGAGGG AGAAGTGCGG CTCGTACTTG GAGAAGGGCT TTGACACTCT AAAGGTCTAT 
GACACACAAC TGGAGAACGT GGAGGCCTTT GGGGGCCTGT CTGACTTTTG TAACACCTTC 
AAGCTCTACC GGGGCAAGAC TCAGGAGGAG ACGGAGGACC CATCTGTTAT CGGGGAGTTT 
AAGGGCCTCT TCAAAATCTA CCCCCTCCCG GAAGACCCGG CCGTCCCCAT GCCCCCGAGA 
CAGTTCCAGC AGCTGGCTGC CCAGGGGCCT CAGGACTGCC TGGTCCGCAT CTATATCATC 
CGGGCATTTG GCCTGCAGCC CAAGGACCCC AATGGGAAGT GTGATCCTTA CATCAAGATC 
TCCATAGGGA AGAAATCAGT GAGTGACCAG GATAATTACA TTCCCTGTAC CCTGGAGCCT 
GTGTTTGGAA AGATGTTCGA GCTGACCTGC ACTCTGCCTC TGGAGAAGGA CCTGAAGATC 
ACACTCTACG ACTATGACCT CCTCTCCAAA GATGAGAAGA TCGGGGAGAC AGTCATCGAC 
CTGGAGAATA GGCTGCTGTC CAAGTTCGGG GCTCGCTGCG GACTCCCACA GACCTACTGT 
GTCTCTGGAC CCAACCAGTG GAGGGACCAG CTCCGCCCCT CCCAGCTTCT CCAGCTCACC 
TGCCAGCAGC GCAGACTCAA GGCGCCCGTG TACCTCACAG ACCGCGTACT CTTTCAGGAT 
AAAGAATACA CCATTGAGGA AATAGAGGCT GGCAGGGTCC TGAACCCACA CCTGGGCCCA 
GTGGAAGAGC GTCTGGCCTT GCACGTCCTC CAGCAGCTGG GCCTGGTCCC CGAGCATGTG 
GAGTCCCGGC CTCTTTACAG CCCCCTGCAG CCAGACATTG AGCAGGGGAA GCTGCAGATG 
TGGATCGATC TGTTTCCCAA GGCCCTGGGG CGACCTGGAC CTCCCTTCAA CATCACCCCA 
CGGAGAGCAA GAAGGTTTTT CCTGCGTTGT ATTATCTGGA ACACAAAGGA CGTGATCCTG 
GACGACCTCA GCATCACGGG GGAGAAGATG AGTGACATTT ACGTGAAAGG TTGGATGGTC 
GGCTTTGAAG AGCACAAGCA GAAGACGGAC GTGCACTACC GCTCCCTGGG AGGCGAAGGC 
AACTTTAACT GGAGGTTCAT TTTCCCCTTT GACTACCTGC CCGCTGAGCA AGTCTGCACC 
GTCTCTAAGA AGGATGCTTT CTGGAGGCTG GACAAGACTG AGAGCAAAAT CCCAGCACAA 
GTGGTATTTC AGATCTGGGA CAATGACAAG TTCTCCTTTG ACGACTTTCT GGGCTCGTTG 
CAGTTGGATC TCAACCGCAT GCCCAAGCCT GCCAAGACAG CTGAGAAGTG CTCCTTGGAC 
CAGCTGGACG ACACTTTCCA CCCGGAGTGG TTTGTGTCCC TTTTTGAACA GAAAACAGTA 
AAGGGCTGGT GGCCCTGTGT GGCAGAAGAC GGTGAGAAGA AGATCCTGGC GGGCAAGCTG 
GAAATGACCT TGGAGATCGT AGCAGAGAGT GAGCACGAAG AGCGGCCTGC GGGTCAAGGC 
CGGGATGAAC CCAACATGAA CCCCAAGCTC GAGGACCCAA GGCGCCCCGA CACCTCCTTC 
CTGTGGTTCA CCTCTCCGTA CAAGACCATG AAGTTCATCC TGTGGCGGCG GTTCCGGTGC 
GCCATCATCC TCTTCGTCGT TCTCGTCATC CTGCTGCTCT TCCTGGGCAT CTTCCTCTAT 
TCCTTCCCGA ACTATGCTGC CATGAAGCTG GTGAAACCCT TCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_019794823.1 ) cDNA ORF clone, Ailuropoda melanoleuca(giant panda) -> XP_019650382.1 Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X4, mRNA.

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CloneID	OAc89677
Clone ID Related Accession (Same CDS sequence)	XM_019794823.1
Accession Version	XM_019794823.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-27
Organism	Ailuropoda melanoleuca(giant panda)
Product	dysferlin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003217356.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ailuropoda melanoleuca Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGTGAAGAA GGACCGGCGC 
AGTGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCAGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTCCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCGG GAGGTCCTCG CCACCCCCAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCCTC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGTGGCTG GTGGGGGACA GAGCCGGGCT 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACGGGAGAT 
GAGGCAGAGC CATTACTGGA TCCGAGCGGT GCCCCAGGCC CTGGGGCCCC CACCACCCCG 
AGGAAGCCAC CTTCCCATCC GCCCCCCTAC CACCCCGGGA GCAAGAGAAA AAGAGGTGCT 
CCCACGCCCA GAAAGCTGCT GTCGGACAAA CCGCAGGACT TCCAGATCAG GGTGCAAGTG 
ATCGAGGGGC GCCAGCTGCC AGGGGTGAAT ATCAAGCCTG TGGTCAAAGT CACGGCTGCC 
GGGCAGACCA AGAGGACACG GATCCACAAG GGAAACAGCC CACTCTTCAA CGAGACTCTC 
TTCTTCAATG TGTTTGACTC TCCCTCGGAG CTGTTTGACG AGCCCATCTT TATCACGGTG 
GTAGACTCCC GTTCTCTCCG GACAGACGCT CTCATTGGGG AATTCCGGAT GGACGTGGGT 
ACCATCTACA GAGAGCCCCG ACACGCCTAT CTGAGGAAAT GGCTTCTCCT CTCGGACCCT 
GACGATTTCT CTGCTGGGGC CAGAGGTTAC CTGAAAGCAA GCCTCTGTGT GCTGGGGCCC 
GGCGATGAAG CGCCTCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATCGAAAAC 
AATCTGCTCA GGCCAACTGG CGTGGCCCTT CGAGGAGCCC ACTTCTGCCT GAAGGTCTTC 
AGAGCTGAGG ACTTACCACA GATGGATGAT GCCGTGATGG ACAACGTGAA GCAGATCTTC 
GGCTTTGACA GTAACAAGAA GAACTTGGTG GATCCCTTCA TGGAAGTCAG CTTTGCGGGG 
AAAATGCTCT GCAGCAAGAT CCTGGAGAAA ACAGCCAACC CTCAGTGGAA CCAGAGCATC 
ACGCTGCCCG CCATGTTTCC CTCCATGTGT GAAAAAATGA GGATTCGCGT CATGGACTGG 
GACCGCCTCA CCCACAATGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCC 
GCCCCTGGAG GAGAAATAGA AGTGGACGAC CATCTGGGCT TCCTCCCCAC CTTCGGGCCC 
TGCTACGTCA ACCTCTACGG CAGCCCCAGG GAGTTCACCG GCTTCCCGGA CCCCTACGCA 
GAACTCAACA TGGGCAAGGG AGAAGGTGTG GCCTACCGAG GTCGGCTTCT GCTCTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGCGACCAA AAGGTGGAGG ACCTCCCTGC GGATGACATC 
CTTCGGGTGG AGAAGTATCT CCGGAGGCGT AAGTACTCCC TCTTTGCCGC CTTCTACTCG 
GCCTCCATGC TCCAGGATGT GGATGATGCC ATCCAGTTCG AGGTCAGCAT CGGGAACTAT 
GGCAACAAGT TCGACACCAC GTGCCTGCCG CTGGCCTCTA CCACCCAGTA CAGCCGGGCG 
GTCTTTGACG GGTGCCACTA CTACTACTTG CCCTGGGGCA ACGTGAAGCC CGTGGTGGTG 
CTGTCATCCT ACTGGGAGGA CATCAGCCAC AGAGTTGAGG CTCAGAACCA GCTGCTCAGG 
ATTGCGGACC GGCTGGAAGC TGCCCTGGAG CAAGTCCACT TGGCCGTGAA GGCGCAGTAC 
AACGCCGAGG ACGTGGACTC CCTGTTGGCT CAGCTGATGG ATGAGCTCAT CTCAGACTGC 
AGCCAGCCCC TAGGCGATGT CCACGAGACA CCCTCTGCCA CCCACCTGGA CCAGTACCTG 
CACAGGCTGC GTAGCCGTCA CCTAAGCCAG ATTGCAGAGG CGGCGCTGGC CCTGAAGCTC 
GGGCACGGCG GGCTCCCGGC CGCCCTGGAG CAGGCTGAAG ACTGGCTCCT GCGGCTCCGT 
GCCCTGGCCG AGGAGCCTCA GAACAGCCTG CCCGACATCA TCATCTGGAT GCTGCAGGGC 
GACAAGCGAG TGGCATACCA GCGGGTGCCT ACACATGAGG TCCTCTTCTC CCGGCGTGGA 
GCCAGCTACT GCGGCAAGAA CTGTGGGAAG CTGCAGACGA TATTTCTCAA ATATCCCATG 
CAGGGGGTGC CCGGAGCCCG GATGCCCGTG CAGATACGGG TGAAGCTCTG GTTTGGGCTT 
TCTGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGAA AGCTGTCTGT CTTTGCTGAA 
ACCTATGAGA ACCAGACCAA ACTGGCCCTC GTTGGGAACT GGGGCACAAC AGGCCTTACC 
TACCCCAAGT TTTCTGATGT CGCGGGCAAG ATCAAGCTCC CCAAGGATAG CTTCCGCCCC 
TCAGCCGGCT GGACCTGGGC TGGAGATTGG TTTGTGTGTC CAGAGAAGAC GCTGCTCCAT 
GACACCGACG CGGGCCACCT GAGTTTCGTG GCGGAGGTGT TTGAGAACCA GACCCGGCTC 
CCTGGAGGCC ACTGGGTCTA CATGAGTGAC AACTACACGG ATGTGAATGG GGAGAAGGTG 
CTTCCCAAGG ATGACATTGA GTGCCCGCTG GGCTGGAAGT GGGAAGATGA AGAGTGGTCC 
ACGGACCTCA ATCGGGCCGT TGATGAGCAA GGCTGGGAGT ACAGTATCAC CATCCCCCCG 
GACCGGAAGC CGAAGCACTG GGTCCCCGCT GAGAAGATGT ACTACACGCA CCGTCGGCGG 
CGCTGGGTCC GCCTGCGCAG GAGGGACCTC AGCCAGATGG AGGCCCTGAG GAAGCACAGG 
CAGGCGGAGG CGGAGGGCGA GGGCTGGGAG TACGCCTCGC TCTTTGGCTG GAAATTCCAC 
CTGGAGTACC GCAAAACGGA TGCATTCCGT CGCCGCCGCT GGCGCCGCCG CATGGAGCCG 
CTGGAGAAGA CGGGGCCTGC AGCCGTGTTT GCCCTTGAGG GGGCACTGGG TGGCGTGGTA 
GATGACCGGA GTGAAGATTC CATGTCCGTG TCCACCTTGA GCTTTGGCGT GAACAGACCC 
ACGATTTCCT GCATCTTTGA TCATGGGAAC CGCTACCACC TGCGCTGCTA TATGTACCAG 
GCTCGGGACC TGCCCTCGAT GGACAAGGAC TCTTTTTCTG ATCCCTACGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACCGTGGTG GTGAAGAACA CTCTGAACCC CACCTGGGAC 
CAGACGCTCA TCTTCTATGA GATTGAGATC TTTGGAGAGC CAGCCAGCAT CGCCGAGCAG 
CCGCCCAGCA TTGTGGTGGA GCTGTATGAC CACGACACCT ACGGTGCAGA TGAGTTTTTG 
GGACGCTGCA TGTGTCAGCC CAGTCTGGAA CGGATGCCCC GGCTGGCCTG GTTCCCGCTG 
GTTAGGGGCA GCCAGCCGGC AGGCGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG 
AAGCCGGCCA TTCACCATAT TCCTGGTTTT GAGGTGCAGG ATACATCGGG AATCCTGGAC 
GAGCTGTGTG CACCTGCCTT CTTCCTCCAG GGCCTCCTCC AAACGTCGGA GGACACAGAC 
CTGCCCTACC CACCGCCTCA GAGGGAGGCC AACATCTACA TGGTTCCCCA GAACATCAAG 
CCGGTTCTCC AGCGCACTGC CATAGAGATC CTGGCATGGG GCCTACGGAA CATGAAGAGT 
TACCAGCTGG CCAGTATCTC CTCCCCCAGC CTCGTGGTGG AGTGCGGGGG CCAGACGGTG 
CAGTCCTGTG TCATCAGGAA CCTCCGGAAG AACCCCAACT TCGACATCTG CACCCTCTTC 
ATGGAAGTGA TGCTGCCCAG AGAGGAGCTC TACTGTCCCC CCATCGTGAT CAAGGTCATT 
GATAACCGCC AGTTTGGCCG CCGGCCTGTG GTGGGCCAGT GTACCATACG CTCCCTGGAG 
AGCTTCATGT GTGACCTGTA CTCCGAGGAG AGTCCGTCCC CACAGGGCGG CCCAGATGAT 
GTGAGTTTAC TCAGTCCTGG GGAAGACGTG CTCATTGACA TCGACGACAA GGAGCCCCTC 
ATCCCCATAC AGCTTACAGA CGGTCTGTTG GGCTCTGCCC CCACTAACAT GGCATCCTCT 
CCATCCAGTC TTCATGAGGA GGAGTTCATC GACTGGTGGA GCAAATTCTT TGCCTCCACA 
GGGGAGAGGG AGAAGTGCGG CTCGTACTTG GAGAAGGGCT TTGACACTCT AAAGGTCTAT 
GACACACAAC TGGAGAACGT GGAGGCCTTT GGGGGCCTGT CTGACTTTTG TAACACCTTC 
AAGCTCTACC GGGGCAAGAC TCAGGAGGAG ACGGAGGACC CATCTGTTAT CGGGGAGTTT 
AAGGGCCTCT TCAAAATCTA CCCCCTCCCG GAAGACCCGG CCGTCCCCAT GCCCCCGAGA 
CAGTTCCAGC AGCTGGCTGC CCAGGGGCCT CAGGACTGCC TGGTCCGCAT CTATATCATC 
CGGGCATTTG GCCTGCAGCC CAAGGACCCC AATGGGAAGT GTGATCCTTA CATCAAGATC 
TCCATAGGGA AGAAATCAGT GAGTGACCAG GATAATTACA TTCCCTGTAC CCTGGAGCCT 
GTGTTTGGAA AGATGTTCGA GCTGACCTGC ACTCTGCCTC TGGAGAAGGA CCTGAAGATC 
ACACTCTACG ACTATGACCT CCTCTCCAAA GATGAGAAGA TCGGGGAGAC AGTCATCGAC 
CTGGAGAATA GGCTGCTGTC CAAGTTCGGG GCTCGCTGCG GACTCCCACA GACCTACTGT 
GTCTCTGGAC CCAACCAGTG GAGGGACCAG CTCCGCCCCT CCCAGCTTCT CCAGCTCACC 
TGCCAGCAGC GCAGACTCAA GGCGCCCGTG TACCTCACAG ACCGCGTACT CTTTCAGGAT 
AAAGAATACA CCATTGAGGA AATAGAGGCT GGCAGGGTCC TGAACCCACA CCTGGGCCCA 
GTGGAAGAGC GTCTGGCCTT GCACGTCCTC CAGCAGCTGG GCCTGGTCCC CGAGCATGTG 
GAGTCCCGGC CTCTTTACAG CCCCCTGCAG CCAGACATTG AGCAGGGGAA GCTGCAGATG 
TGGATCGATC TGTTTCCCAA GGCCCTGGGG CGACCTGGAC CTCCCTTCAA CATCACCCCA 
CGGAGAGCAA GAAGGTTTTT CCTGCGTTGT ATTATCTGGA ACACAAAGGA CGTGATCCTG 
GACGACCTCA GCATCACGGG GGAGAAGATG AGTGACATTT ACGTGAAAGG TTGGATGGTC 
GGCTTTGAAG AGCACAAGCA GAAGACGGAC GTGCACTACC GCTCCCTGGG AGGCGAAGGC 
AACTTTAACT GGAGGTTCAT TTTCCCCTTT GACTACCTGC CCGCTGAGCA AGTCTGCACC 
GTCTCTAAGA AGGATGCTTT CTGGAGGCTG GACAAGACTG AGAGCAAAAT CCCAGCACAA 
GTGGTATTTC AGATCTGGGA CAATGACAAG TTCTCCTTTG ACGACTTTCT GGGCTCGTTG 
CAGTTGGATC TCAACCGCAT GCCCAAGCCT GCCAAGACAG CTGAGAAGTG CTCCTTGGAC 
CAGCTGGACG ACACTTTCCA CCCGGAGTGG TTTGTGTCCC TTTTTGAACA GAAAACAGTA 
AAGGGCTGGT GGCCCTGTGT GGCAGAAGAC GGTGAGAAGA AGATCCTGGC GGGCAAGCTG 
GAAATGACCT TGGAGATCGT AGCAGAGAGT GAGCACGAAG AGCGGCCTGC GGGTCAAGGC 
CGGGATGAAC CCAACATGAA CCCCAAGCTC GAGGACCCAA GGCGCCCCGA CACCTCCTTC 
CTGTGGTTCA CCTCTCCGTA CAAGACCATG AAGTTCATCC TGTGGCGGCG GTTCCGGTGC 
GCCATCATCC TCTTCGTCGT TCTCGTCATC CTGCTGCTCT TCCTGGGCAT CTTCCTCTAT 
TCCTTCCCGA ACTATGCTGC CATGAAGCTG GTGAAACCCT TCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019650382.1
CDS	150..6557
Translation	MLSCLLVRASNLPSVKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEAQVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPSASLEPSPTLPDMDMVAGGGQSRAETWSLLSDSTVDTRYSGKKWLAPTDTGGEEDTEDQGLTGDEAEPLLDPSGAPGPGAPTTPRKPPSHPPPYHPGSKRKRGAPTPRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVMDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDHLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRLLLSLETKLVEHSDQKVEDLPADDILRVEKYLRRRKYSLFAAFYSASMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADRLEAALEQVHLAVKAQYNAEDVDSLLAQLMDELISDCSQPLGDVHETPSATHLDQYLHRLRSRHLSQIAEAALALKLGHGGLPAALEQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPTHEVLFSRRGASYCGKNCGKLQTIFLKYPMQGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVAGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDTDAGHLSFVAEVFENQTRLPGGHWVYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALRKHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSMSVSTLSFGVNRPTISCIFDHGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFLGRCMCQPSLERMPRLAWFPLVRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTSGILDELCAPAFFLQGLLQTSEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPIVIKVIDNRQFGRRPVVGQCTIRSLESFMCDLYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLTDGLLGSAPTNMASSPSSLHEEEFIDWWSKFFASTGEREKCGSYLEKGFDTLKVYDTQLENVEAFGGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAVPMPPRQFQQLAAQGPQDCLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLQLTCQQRRLKAPVYLTDRVLFQDKEYTIEEIEAGRVLNPHLGPVEERLALHVLQQLGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPAQVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEDGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFVVLVILLLFLGIFLYSFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_019794823.1
Organism	Ailuropoda melanoleuca(giant panda)
Definition	Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019794823.1

ORF Insert Sequence:

ATGCTGAGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGTGAAGAA GGACCGGCGC 
AGTGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCAGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTCCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCGG GAGGTCCTCG CCACCCCCAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCCTC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGTGGCTG GTGGGGGACA GAGCCGGGCT 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACGGGAGAT 
GAGGCAGAGC CATTACTGGA TCCGAGCGGT GCCCCAGGCC CTGGGGCCCC CACCACCCCG 
AGGAAGCCAC CTTCCCATCC GCCCCCCTAC CACCCCGGGA GCAAGAGAAA AAGAGGTGCT 
CCCACGCCCA GAAAGCTGCT GTCGGACAAA CCGCAGGACT TCCAGATCAG GGTGCAAGTG 
ATCGAGGGGC GCCAGCTGCC AGGGGTGAAT ATCAAGCCTG TGGTCAAAGT CACGGCTGCC 
GGGCAGACCA AGAGGACACG GATCCACAAG GGAAACAGCC CACTCTTCAA CGAGACTCTC 
TTCTTCAATG TGTTTGACTC TCCCTCGGAG CTGTTTGACG AGCCCATCTT TATCACGGTG 
GTAGACTCCC GTTCTCTCCG GACAGACGCT CTCATTGGGG AATTCCGGAT GGACGTGGGT 
ACCATCTACA GAGAGCCCCG ACACGCCTAT CTGAGGAAAT GGCTTCTCCT CTCGGACCCT 
GACGATTTCT CTGCTGGGGC CAGAGGTTAC CTGAAAGCAA GCCTCTGTGT GCTGGGGCCC 
GGCGATGAAG CGCCTCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATCGAAAAC 
AATCTGCTCA GGCCAACTGG CGTGGCCCTT CGAGGAGCCC ACTTCTGCCT GAAGGTCTTC 
AGAGCTGAGG ACTTACCACA GATGGATGAT GCCGTGATGG ACAACGTGAA GCAGATCTTC 
GGCTTTGACA GTAACAAGAA GAACTTGGTG GATCCCTTCA TGGAAGTCAG CTTTGCGGGG 
AAAATGCTCT GCAGCAAGAT CCTGGAGAAA ACAGCCAACC CTCAGTGGAA CCAGAGCATC 
ACGCTGCCCG CCATGTTTCC CTCCATGTGT GAAAAAATGA GGATTCGCGT CATGGACTGG 
GACCGCCTCA CCCACAATGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCC 
GCCCCTGGAG GAGAAATAGA AGTGGACGAC CATCTGGGCT TCCTCCCCAC CTTCGGGCCC 
TGCTACGTCA ACCTCTACGG CAGCCCCAGG GAGTTCACCG GCTTCCCGGA CCCCTACGCA 
GAACTCAACA TGGGCAAGGG AGAAGGTGTG GCCTACCGAG GTCGGCTTCT GCTCTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGCGACCAA AAGGTGGAGG ACCTCCCTGC GGATGACATC 
CTTCGGGTGG AGAAGTATCT CCGGAGGCGT AAGTACTCCC TCTTTGCCGC CTTCTACTCG 
GCCTCCATGC TCCAGGATGT GGATGATGCC ATCCAGTTCG AGGTCAGCAT CGGGAACTAT 
GGCAACAAGT TCGACACCAC GTGCCTGCCG CTGGCCTCTA CCACCCAGTA CAGCCGGGCG 
GTCTTTGACG GGTGCCACTA CTACTACTTG CCCTGGGGCA ACGTGAAGCC CGTGGTGGTG 
CTGTCATCCT ACTGGGAGGA CATCAGCCAC AGAGTTGAGG CTCAGAACCA GCTGCTCAGG 
ATTGCGGACC GGCTGGAAGC TGCCCTGGAG CAAGTCCACT TGGCCGTGAA GGCGCAGTAC 
AACGCCGAGG ACGTGGACTC CCTGTTGGCT CAGCTGATGG ATGAGCTCAT CTCAGACTGC 
AGCCAGCCCC TAGGCGATGT CCACGAGACA CCCTCTGCCA CCCACCTGGA CCAGTACCTG 
CACAGGCTGC GTAGCCGTCA CCTAAGCCAG ATTGCAGAGG CGGCGCTGGC CCTGAAGCTC 
GGGCACGGCG GGCTCCCGGC CGCCCTGGAG CAGGCTGAAG ACTGGCTCCT GCGGCTCCGT 
GCCCTGGCCG AGGAGCCTCA GAACAGCCTG CCCGACATCA TCATCTGGAT GCTGCAGGGC 
GACAAGCGAG TGGCATACCA GCGGGTGCCT ACACATGAGG TCCTCTTCTC CCGGCGTGGA 
GCCAGCTACT GCGGCAAGAA CTGTGGGAAG CTGCAGACGA TATTTCTCAA ATATCCCATG 
CAGGGGGTGC CCGGAGCCCG GATGCCCGTG CAGATACGGG TGAAGCTCTG GTTTGGGCTT 
TCTGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGAA AGCTGTCTGT CTTTGCTGAA 
ACCTATGAGA ACCAGACCAA ACTGGCCCTC GTTGGGAACT GGGGCACAAC AGGCCTTACC 
TACCCCAAGT TTTCTGATGT CGCGGGCAAG ATCAAGCTCC CCAAGGATAG CTTCCGCCCC 
TCAGCCGGCT GGACCTGGGC TGGAGATTGG TTTGTGTGTC CAGAGAAGAC GCTGCTCCAT 
GACACCGACG CGGGCCACCT GAGTTTCGTG GCGGAGGTGT TTGAGAACCA GACCCGGCTC 
CCTGGAGGCC ACTGGGTCTA CATGAGTGAC AACTACACGG ATGTGAATGG GGAGAAGGTG 
CTTCCCAAGG ATGACATTGA GTGCCCGCTG GGCTGGAAGT GGGAAGATGA AGAGTGGTCC 
ACGGACCTCA ATCGGGCCGT TGATGAGCAA GGCTGGGAGT ACAGTATCAC CATCCCCCCG 
GACCGGAAGC CGAAGCACTG GGTCCCCGCT GAGAAGATGT ACTACACGCA CCGTCGGCGG 
CGCTGGGTCC GCCTGCGCAG GAGGGACCTC AGCCAGATGG AGGCCCTGAG GAAGCACAGG 
CAGGCGGAGG CGGAGGGCGA GGGCTGGGAG TACGCCTCGC TCTTTGGCTG GAAATTCCAC 
CTGGAGTACC GCAAAACGGA TGCATTCCGT CGCCGCCGCT GGCGCCGCCG CATGGAGCCG 
CTGGAGAAGA CGGGGCCTGC AGCCGTGTTT GCCCTTGAGG GGGCACTGGG TGGCGTGGTA 
GATGACCGGA GTGAAGATTC CATGTCCGTG TCCACCTTGA GCTTTGGCGT GAACAGACCC 
ACGATTTCCT GCATCTTTGA TCATGGGAAC CGCTACCACC TGCGCTGCTA TATGTACCAG 
GCTCGGGACC TGCCCTCGAT GGACAAGGAC TCTTTTTCTG ATCCCTACGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACCGTGGTG GTGAAGAACA CTCTGAACCC CACCTGGGAC 
CAGACGCTCA TCTTCTATGA GATTGAGATC TTTGGAGAGC CAGCCAGCAT CGCCGAGCAG 
CCGCCCAGCA TTGTGGTGGA GCTGTATGAC CACGACACCT ACGGTGCAGA TGAGTTTTTG 
GGACGCTGCA TGTGTCAGCC CAGTCTGGAA CGGATGCCCC GGCTGGCCTG GTTCCCGCTG 
GTTAGGGGCA GCCAGCCGGC AGGCGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG 
AAGCCGGCCA TTCACCATAT TCCTGGTTTT GAGGTGCAGG ATACATCGGG AATCCTGGAC 
GAGCTGTGTG CACCTGCCTT CTTCCTCCAG GGCCTCCTCC AAACGTCGGA GGACACAGAC 
CTGCCCTACC CACCGCCTCA GAGGGAGGCC AACATCTACA TGGTTCCCCA GAACATCAAG 
CCGGTTCTCC AGCGCACTGC CATAGAGATC CTGGCATGGG GCCTACGGAA CATGAAGAGT 
TACCAGCTGG CCAGTATCTC CTCCCCCAGC CTCGTGGTGG AGTGCGGGGG CCAGACGGTG 
CAGTCCTGTG TCATCAGGAA CCTCCGGAAG AACCCCAACT TCGACATCTG CACCCTCTTC 
ATGGAAGTGA TGCTGCCCAG AGAGGAGCTC TACTGTCCCC CCATCGTGAT CAAGGTCATT 
GATAACCGCC AGTTTGGCCG CCGGCCTGTG GTGGGCCAGT GTACCATACG CTCCCTGGAG 
AGCTTCATGT GTGACCTGTA CTCCGAGGAG AGTCCGTCCC CACAGGGCGG CCCAGATGAT 
GTGAGTTTAC TCAGTCCTGG GGAAGACGTG CTCATTGACA TCGACGACAA GGAGCCCCTC 
ATCCCCATAC AGCTTACAGA CGGTCTGTTG GGCTCTGCCC CCACTAACAT GGCATCCTCT 
CCATCCAGTC TTCATGAGGA GGAGTTCATC GACTGGTGGA GCAAATTCTT TGCCTCCACA 
GGGGAGAGGG AGAAGTGCGG CTCGTACTTG GAGAAGGGCT TTGACACTCT AAAGGTCTAT 
GACACACAAC TGGAGAACGT GGAGGCCTTT GGGGGCCTGT CTGACTTTTG TAACACCTTC 
AAGCTCTACC GGGGCAAGAC TCAGGAGGAG ACGGAGGACC CATCTGTTAT CGGGGAGTTT 
AAGGGCCTCT TCAAAATCTA CCCCCTCCCG GAAGACCCGG CCGTCCCCAT GCCCCCGAGA 
CAGTTCCAGC AGCTGGCTGC CCAGGGGCCT CAGGACTGCC TGGTCCGCAT CTATATCATC 
CGGGCATTTG GCCTGCAGCC CAAGGACCCC AATGGGAAGT GTGATCCTTA CATCAAGATC 
TCCATAGGGA AGAAATCAGT GAGTGACCAG GATAATTACA TTCCCTGTAC CCTGGAGCCT 
GTGTTTGGAA AGATGTTCGA GCTGACCTGC ACTCTGCCTC TGGAGAAGGA CCTGAAGATC 
ACACTCTACG ACTATGACCT CCTCTCCAAA GATGAGAAGA TCGGGGAGAC AGTCATCGAC 
CTGGAGAATA GGCTGCTGTC CAAGTTCGGG GCTCGCTGCG GACTCCCACA GACCTACTGT 
GTCTCTGGAC CCAACCAGTG GAGGGACCAG CTCCGCCCCT CCCAGCTTCT CCAGCTCACC 
TGCCAGCAGC GCAGACTCAA GGCGCCCGTG TACCTCACAG ACCGCGTACT CTTTCAGGAT 
AAAGAATACA CCATTGAGGA AATAGAGGCT GGCAGGGTCC TGAACCCACA CCTGGGCCCA 
GTGGAAGAGC GTCTGGCCTT GCACGTCCTC CAGCAGCTGG GCCTGGTCCC CGAGCATGTG 
GAGTCCCGGC CTCTTTACAG CCCCCTGCAG CCAGACATTG AGCAGGGGAA GCTGCAGATG 
TGGATCGATC TGTTTCCCAA GGCCCTGGGG CGACCTGGAC CTCCCTTCAA CATCACCCCA 
CGGAGAGCAA GAAGGTTTTT CCTGCGTTGT ATTATCTGGA ACACAAAGGA CGTGATCCTG 
GACGACCTCA GCATCACGGG GGAGAAGATG AGTGACATTT ACGTGAAAGG TTGGATGGTC 
GGCTTTGAAG AGCACAAGCA GAAGACGGAC GTGCACTACC GCTCCCTGGG AGGCGAAGGC 
AACTTTAACT GGAGGTTCAT TTTCCCCTTT GACTACCTGC CCGCTGAGCA AGTCTGCACC 
GTCTCTAAGA AGGATGCTTT CTGGAGGCTG GACAAGACTG AGAGCAAAAT CCCAGCACAA 
GTGGTATTTC AGATCTGGGA CAATGACAAG TTCTCCTTTG ACGACTTTCT GGGCTCGTTG 
CAGTTGGATC TCAACCGCAT GCCCAAGCCT GCCAAGACAG CTGAGAAGTG CTCCTTGGAC 
CAGCTGGACG ACACTTTCCA CCCGGAGTGG TTTGTGTCCC TTTTTGAACA GAAAACAGTA 
AAGGGCTGGT GGCCCTGTGT GGCAGAAGAC GGTGAGAAGA AGATCCTGGC GGGCAAGCTG 
GAAATGACCT TGGAGATCGT AGCAGAGAGT GAGCACGAAG AGCGGCCTGC GGGTCAAGGC 
CGGGATGAAC CCAACATGAA CCCCAAGCTC GAGGACCCAA GGCGCCCCGA CACCTCCTTC 
CTGTGGTTCA CCTCTCCGTA CAAGACCATG AAGTTCATCC TGTGGCGGCG GTTCCGGTGC 
GCCATCATCC TCTTCGTCGT TCTCGTCATC CTGCTGCTCT TCCTGGGCAT CTTCCTCTAT 
TCCTTCCCGA ACTATGCTGC CATGAAGCTG GTGAAACCCT TCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_019794824.1 ) cDNA ORF clone, Ailuropoda melanoleuca(giant panda) -> XP_019650383.1 Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X5, mRNA.

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CloneID	OAc89678
Clone ID Related Accession (Same CDS sequence)	XM_019794824.1
Accession Version	XM_019794824.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-27
Organism	Ailuropoda melanoleuca(giant panda)
Product	dysferlin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003217356.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ailuropoda melanoleuca Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGTGAAGAA GGACCGGCGC 
AGTGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCAGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTCCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCGG GAGGTCCTCG CCACCCCCAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCCTC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGTGGCTG GTGGGGGACA GAGCCGGGCT 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACGGGAGAT 
GAGGCAGAGC CATTACTGGA TCCGAGCGGT GCCCCAGGCC CTGGGGCCCC CACCACCCCG 
AGGAAGCCAC CTTCCCATCC GCCCCCCTAC CACCCCGGGA GCAAGAGAAA AAGAGGTGCT 
CCCACGCCCA GAAAGCTGCT GTCGGACAAA CCGCAGGACT TCCAGATCAG GGTGCAAGTG 
ATCGAGGGGC GCCAGCTGCC AGGGGTGAAT ATCAAGCCTG TGGTCAAAGT CACGGCTGCC 
GGGCAGACCA AGAGGACACG GATCCACAAG GGAAACAGCC CACTCTTCAA CGAGACTCTC 
TTCTTCAATG TGTTTGACTC TCCCTCGGAG CTGTTTGACG AGCCCATCTT TATCACGGTG 
GTAGACTCCC GTTCTCTCCG GACAGACGCT CTCATTGGGG AATTCCGGAT GGACGTGGGT 
ACCATCTACA GAGAGCCCCG ACACGCCTAT CTGAGGAAAT GGCTTCTCCT CTCGGACCCT 
GACGATTTCT CTGCTGGGGC CAGAGGTTAC CTGAAAGCAA GCCTCTGTGT GCTGGGGCCC 
GGCGATGAAG CGCCTCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATCGAAAAC 
AATCTGCTCA GGCCAACTGG CGTGGCCCTT CGAGGAGCCC ACTTCTGCCT GAAGGTCTTC 
AGAGCTGAGG ACTTACCACA GATGGATGAT GCCGTGATGG ACAACGTGAA GCAGATCTTC 
GGCTTTGACA GTAACAAGAA GAACTTGGTG GATCCCTTCA TGGAAGTCAG CTTTGCGGGG 
AAAATGCTCT GCAGCAAGAT CCTGGAGAAA ACAGCCAACC CTCAGTGGAA CCAGAGCATC 
ACGCTGCCCG CCATGTTTCC CTCCATGTGT GAAAAAATGA GGATTCGCGT CATGGACTGG 
GACCGCCTCA CCCACAATGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCC 
GCCCCTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTGG CCAAGCCTTC TCCAGCCATG 
GACCTGGACG ACCATCTGGG CTTCCTCCCC ACCTTCGGGC CCTGCTACGT CAACCTCTAC 
GGCAGCCCCA GGGAGTTCAC CGGCTTCCCG GACCCCTACG CAGAACTCAA CATGGGCAAG 
GGAGAAGGTG TGGCCTACCG AGGTCGGCTT CTGCTCTCCC TGGAGACCAA GCTGGTGGAG 
CACAGCGACC AAAAGGTGGA GGACCTCCCT GCGGATGACA TCCTTCGGGT GGAGAAGTAT 
CTCCGGAGGC GTAAGTACTC CCTCTTTGCC GCCTTCTACT CGGCCTCCAT GCTCCAGGAT 
GTGGATGATG CCATCCAGTT CGAGGTCAGC ATCGGGAACT ATGGCAACAA GTTCGACACC 
ACGTGCCTGC CGCTGGCCTC TACCACCCAG TACAGCCGGG CGGTCTTTGA CGGGTGCCAC 
TACTACTACT TGCCCTGGGG CAACGTGAAG CCCGTGGTGG TGCTGTCATC CTACTGGGAG 
GACATCAGCC ACAGAGTTGA GGCTCAGAAC CAGCTGCTCA GGATTGCGGA CCGGCTGGAA 
GCTGCCCTGG AGCAAGTCCA CTTGGCCGTG AAGGCGCAGT ACAACGCCGA GGACGTGGAC 
TCCCTGTTGG CTCAGCTGAT GGATGAGCTC ATCTCAGACT GCAGCCAGCC CCTAGGCGAT 
GTCCACGAGA CACCCTCTGC CACCCACCTG GACCAGTACC TGCACAGGCT GCGTAGCCGT 
CACCTAAGCC AGATTGCAGA GGCGGCGCTG GCCCTGAAGC TCGGGCACGG CGGGCTCCCG 
GCCGCCCTGG AGCAGGCTGA AGACTGGCTC CTGCGGCTCC GTGCCCTGGC CGAGGAGCCT 
CAGAACAGCC TGCCCGACAT CATCATCTGG ATGCTGCAGG GCGACAAGCG AGTGGCATAC 
CAGCGGGTGC CTACACATGA GGTCCTCTTC TCCCGGCGTG GAGCCAGCTA CTGCGGCAAG 
AACTGTGGGA AGCTGCAGAC GATATTTCTC AAATATCCCA TGCAGGGGGT GCCCGGAGCC 
CGGATGCCCG TGCAGATACG GGTGAAGCTC TGGTTTGGGC TTTCTGTGGA TGAGAAGGAG 
TTCAACCAGT TTGCTGAGGG AAAGCTGTCT GTCTTTGCTG AAACCTATGA GAACCAGACC 
AAACTGGCCC TCGTTGGGAA CTGGGGCACA ACAGGCCTTA CCTACCCCAA GTTTTCTGAT 
GTCGCGGGCA AGATCAAGCT CCCCAAGGAT AGCTTCCGCC CCTCAGCCGG CTGGACCTGG 
GCTGGAGATT GGTTTGTGTG TCCAGAGAAG ACGCTGCTCC ATGACACCGA CGCGGGCCAC 
CTGAGTTTCG TGGCGGAGGT GTTTGAGAAC CAGACCCGGC TCCCTGGAGG CCACTGGGTC 
TACATGAGTG ACAACTACAC GGATGTGAAT GGGGAGAAGG TGCTTCCCAA GGATGACATT 
GAGTGCCCGC TGGGCTGGAA GTGGGAAGAT GAAGAGTGGT CCACGGACCT CAATCGGGCC 
GTTGATGAGC AAGGCTGGGA GTACAGTATC ACCATCCCCC CGGACCGGAA GCCGAAGCAC 
TGGGTCCCCG CTGAGAAGAT GTACTACACG CACCGTCGGC GGCGCTGGGT CCGCCTGCGC 
AGGAGGGACC TCAGCCAGAT GGAGGCCCTG AGGAAGCACA GGCAGGCGGA GGCGGAGGGC 
GAGGGCTGGG AGTACGCCTC GCTCTTTGGC TGGAAATTCC ACCTGGAGTA CCGCAAAACG 
GATGCATTCC GTCGCCGCCG CTGGCGCCGC CGCATGGAGC CGCTGGAGAA GACGGGGCCT 
GCAGCCGTGT TTGCCCTTGA GGGGGCACTG GGTGGCGTGG TAGATGACCG GAGTGAAGAT 
TCCATGTCCG TGTCCACCTT GAGCTTTGGC GTGAACAGAC CCACGATTTC CTGCATCTTT 
GATCATGGGA ACCGCTACCA CCTGCGCTGC TATATGTACC AGGCTCGGGA CCTGCCCTCG 
ATGGACAAGG ACTCTTTTTC TGATCCCTAC GCCATCGTCT CCTTCCTGCA CCAGAGCCAG 
AAGACCGTGG TGGTGAAGAA CACTCTGAAC CCCACCTGGG ACCAGACGCT CATCTTCTAT 
GAGATTGAGA TCTTTGGAGA GCCAGCCAGC ATCGCCGAGC AGCCGCCCAG CATTGTGGTG 
GAGCTGTATG ACCACGACAC CTACGGTGCA GATGAGTTTT TGGGACGCTG CATGTGTCAG 
CCCAGTCTGG AACGGATGCC CCGGCTGGCC TGGTTCCCGC TGGTTAGGGG CAGCCAGCCG 
GCAGGCGAGC TGCTGGCCTC TTTTGAGCTC ATCCAGAGAG AGAAGCCGGC CATTCACCAT 
ATTCCTGGTT TTGAGGTGCA GGATACATCG GGAATCCTGG ACGAGCTGTG TGCACCTGCC 
TTCTTCCTCC AGGGCCTCCT CCAAACGTCG GAGGACACAG ACCTGCCCTA CCCACCGCCT 
CAGAGGGAGG CCAACATCTA CATGGTTCCC CAGAACATCA AGCCGGTTCT CCAGCGCACT 
GCCATAGAGA TCCTGGCATG GGGCCTACGG AACATGAAGA GTTACCAGCT GGCCAGTATC 
TCCTCCCCCA GCCTCGTGGT GGAGTGCGGG GGCCAGACGG TGCAGTCCTG TGTCATCAGG 
AACCTCCGGA AGAACCCCAA CTTCGACATC TGCACCCTCT TCATGGAAGT GATGCTGCCC 
AGAGAGGAGC TCTACTGTCC CCCCATCGTG ATCAAGGTCA TTGATAACCG CCAGTTTGGC 
CGCCGGCCTG TGGTGGGCCA GTGTACCATA CGCTCCCTGG AGAGCTTCAT GTGTGACCTG 
TACTCCGAGG AGAGTCCGTC CCCACAGGGC GGCCCAGATG ATGTGAGTTT ACTCAGTCCT 
GGGGAAGACG TGCTCATTGA CATCGACGAC AAGGAGCCCC TCATCCCCAT ACAGGAGGAG 
GAGTTCATCG ACTGGTGGAG CAAATTCTTT GCCTCCACAG GGGAGAGGGA GAAGTGCGGC 
TCGTACTTGG AGAAGGGCTT TGACACTCTA AAGGTCTATG ACACACAACT GGAGAACGTG 
GAGGCCTTTG GGGGCCTGTC TGACTTTTGT AACACCTTCA AGCTCTACCG GGGCAAGACT 
CAGGAGGAGA CGGAGGACCC ATCTGTTATC GGGGAGTTTA AGGGCCTCTT CAAAATCTAC 
CCCCTCCCGG AAGACCCGGC CGTCCCCATG CCCCCGAGAC AGTTCCAGCA GCTGGCTGCC 
CAGGGGCCTC AGGACTGCCT GGTCCGCATC TATATCATCC GGGCATTTGG CCTGCAGCCC 
AAGGACCCCA ATGGGAAGTG TGATCCTTAC ATCAAGATCT CCATAGGGAA GAAATCAGTG 
AGTGACCAGG ATAATTACAT TCCCTGTACC CTGGAGCCTG TGTTTGGAAA GATGTTCGAG 
CTGACCTGCA CTCTGCCTCT GGAGAAGGAC CTGAAGATCA CACTCTACGA CTATGACCTC 
CTCTCCAAAG ATGAGAAGAT CGGGGAGACA GTCATCGACC TGGAGAATAG GCTGCTGTCC 
AAGTTCGGGG CTCGCTGCGG ACTCCCACAG ACCTACTGTG TCTCTGGACC CAACCAGTGG 
AGGGACCAGC TCCGCCCCTC CCAGCTTCTC CAGCTCACCT GCCAGCAGCG CAGACTCAAG 
GCGCCCGTGT ACCTCACAGA CCGCGTACTC TTTCAGGATA AAGAATACAC CATTGAGGAA 
ATAGAGGCTG GCAGGGTCCT GAACCCACAC CTGGGCCCAG TGGAAGAGCG TCTGGCCTTG 
CACGTCCTCC AGCAGCTGGG CCTGGTCCCC GAGCATGTGG AGTCCCGGCC TCTTTACAGC 
CCCCTGCAGC CAGACATTGA GCAGGGGAAG CTGCAGATGT GGATCGATCT GTTTCCCAAG 
GCCCTGGGGC GACCTGGACC TCCCTTCAAC ATCACCCCAC GGAGAGCAAG AAGGTTTTTC 
CTGCGTTGTA TTATCTGGAA CACAAAGGAC GTGATCCTGG ACGACCTCAG CATCACGGGG 
GAGAAGATGA GTGACATTTA CGTGAAAGGT TGGATGGTCG GCTTTGAAGA GCACAAGCAG 
AAGACGGACG TGCACTACCG CTCCCTGGGA GGCGAAGGCA ACTTTAACTG GAGGTTCATT 
TTCCCCTTTG ACTACCTGCC CGCTGAGCAA GTCTGCACCG TCTCTAAGAA GGATGCTTTC 
TGGAGGCTGG ACAAGACTGA GAGCAAAATC CCAGCACAAG TGGTATTTCA GATCTGGGAC 
AATGACAAGT TCTCCTTTGA CGACTTTCTG GGCTCGTTGC AGTTGGATCT CAACCGCATG 
CCCAAGCCTG CCAAGACAGC TGAGAAGTGC TCCTTGGACC AGCTGGACGA CACTTTCCAC 
CCGGAGTGGT TTGTGTCCCT TTTTGAACAG AAAACAGTAA AGGGCTGGTG GCCCTGTGTG 
GCAGAAGACG GTGAGAAGAA GATCCTGGCG GGCAAGCTGG AAATGACCTT GGAGATCGTA 
GCAGAGAGTG AGCACGAAGA GCGGCCTGCG GGTCAAGGCC GGGATGAACC CAACATGAAC 
CCCAAGCTCG AGGACCCAAG GCGCCCCGAC ACCTCCTTCC TGTGGTTCAC CTCTCCGTAC 
AAGACCATGA AGTTCATCCT GTGGCGGCGG TTCCGGTGCG CCATCATCCT CTTCGTCGTT 
CTCGTCATCC TGCTGCTCTT CCTGGGCATC TTCCTCTATT CCTTCCCGAA CTATGCTGCC 
ATGAAGCTGG TGAAACCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019650383.1
CDS	150..6536
Translation	MLSCLLVRASNLPSVKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEAQVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPSASLEPSPTLPDMDMVAGGGQSRAETWSLLSDSTVDTRYSGKKWLAPTDTGGEEDTEDQGLTGDEAEPLLDPSGAPGPGAPTTPRKPPSHPPPYHPGSKRKRGAPTPRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVMDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGVAKPSPAMDLDDHLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRLLLSLETKLVEHSDQKVEDLPADDILRVEKYLRRRKYSLFAAFYSASMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADRLEAALEQVHLAVKAQYNAEDVDSLLAQLMDELISDCSQPLGDVHETPSATHLDQYLHRLRSRHLSQIAEAALALKLGHGGLPAALEQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPTHEVLFSRRGASYCGKNCGKLQTIFLKYPMQGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVAGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDTDAGHLSFVAEVFENQTRLPGGHWVYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALRKHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSMSVSTLSFGVNRPTISCIFDHGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFLGRCMCQPSLERMPRLAWFPLVRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTSGILDELCAPAFFLQGLLQTSEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPIVIKVIDNRQFGRRPVVGQCTIRSLESFMCDLYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASTGEREKCGSYLEKGFDTLKVYDTQLENVEAFGGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAVPMPPRQFQQLAAQGPQDCLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLQLTCQQRRLKAPVYLTDRVLFQDKEYTIEEIEAGRVLNPHLGPVEERLALHVLQQLGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPAQVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEDGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFVVLVILLLFLGIFLYSFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_019794824.1
Organism	Ailuropoda melanoleuca(giant panda)
Definition	Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019794824.1

ORF Insert Sequence:

ATGCTGAGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGTGAAGAA GGACCGGCGC 
AGTGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCAGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTCCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCGG GAGGTCCTCG CCACCCCCAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCCTC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGTGGCTG GTGGGGGACA GAGCCGGGCT 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACGGGAGAT 
GAGGCAGAGC CATTACTGGA TCCGAGCGGT GCCCCAGGCC CTGGGGCCCC CACCACCCCG 
AGGAAGCCAC CTTCCCATCC GCCCCCCTAC CACCCCGGGA GCAAGAGAAA AAGAGGTGCT 
CCCACGCCCA GAAAGCTGCT GTCGGACAAA CCGCAGGACT TCCAGATCAG GGTGCAAGTG 
ATCGAGGGGC GCCAGCTGCC AGGGGTGAAT ATCAAGCCTG TGGTCAAAGT CACGGCTGCC 
GGGCAGACCA AGAGGACACG GATCCACAAG GGAAACAGCC CACTCTTCAA CGAGACTCTC 
TTCTTCAATG TGTTTGACTC TCCCTCGGAG CTGTTTGACG AGCCCATCTT TATCACGGTG 
GTAGACTCCC GTTCTCTCCG GACAGACGCT CTCATTGGGG AATTCCGGAT GGACGTGGGT 
ACCATCTACA GAGAGCCCCG ACACGCCTAT CTGAGGAAAT GGCTTCTCCT CTCGGACCCT 
GACGATTTCT CTGCTGGGGC CAGAGGTTAC CTGAAAGCAA GCCTCTGTGT GCTGGGGCCC 
GGCGATGAAG CGCCTCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATCGAAAAC 
AATCTGCTCA GGCCAACTGG CGTGGCCCTT CGAGGAGCCC ACTTCTGCCT GAAGGTCTTC 
AGAGCTGAGG ACTTACCACA GATGGATGAT GCCGTGATGG ACAACGTGAA GCAGATCTTC 
GGCTTTGACA GTAACAAGAA GAACTTGGTG GATCCCTTCA TGGAAGTCAG CTTTGCGGGG 
AAAATGCTCT GCAGCAAGAT CCTGGAGAAA ACAGCCAACC CTCAGTGGAA CCAGAGCATC 
ACGCTGCCCG CCATGTTTCC CTCCATGTGT GAAAAAATGA GGATTCGCGT CATGGACTGG 
GACCGCCTCA CCCACAATGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCC 
GCCCCTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTGG CCAAGCCTTC TCCAGCCATG 
GACCTGGACG ACCATCTGGG CTTCCTCCCC ACCTTCGGGC CCTGCTACGT CAACCTCTAC 
GGCAGCCCCA GGGAGTTCAC CGGCTTCCCG GACCCCTACG CAGAACTCAA CATGGGCAAG 
GGAGAAGGTG TGGCCTACCG AGGTCGGCTT CTGCTCTCCC TGGAGACCAA GCTGGTGGAG 
CACAGCGACC AAAAGGTGGA GGACCTCCCT GCGGATGACA TCCTTCGGGT GGAGAAGTAT 
CTCCGGAGGC GTAAGTACTC CCTCTTTGCC GCCTTCTACT CGGCCTCCAT GCTCCAGGAT 
GTGGATGATG CCATCCAGTT CGAGGTCAGC ATCGGGAACT ATGGCAACAA GTTCGACACC 
ACGTGCCTGC CGCTGGCCTC TACCACCCAG TACAGCCGGG CGGTCTTTGA CGGGTGCCAC 
TACTACTACT TGCCCTGGGG CAACGTGAAG CCCGTGGTGG TGCTGTCATC CTACTGGGAG 
GACATCAGCC ACAGAGTTGA GGCTCAGAAC CAGCTGCTCA GGATTGCGGA CCGGCTGGAA 
GCTGCCCTGG AGCAAGTCCA CTTGGCCGTG AAGGCGCAGT ACAACGCCGA GGACGTGGAC 
TCCCTGTTGG CTCAGCTGAT GGATGAGCTC ATCTCAGACT GCAGCCAGCC CCTAGGCGAT 
GTCCACGAGA CACCCTCTGC CACCCACCTG GACCAGTACC TGCACAGGCT GCGTAGCCGT 
CACCTAAGCC AGATTGCAGA GGCGGCGCTG GCCCTGAAGC TCGGGCACGG CGGGCTCCCG 
GCCGCCCTGG AGCAGGCTGA AGACTGGCTC CTGCGGCTCC GTGCCCTGGC CGAGGAGCCT 
CAGAACAGCC TGCCCGACAT CATCATCTGG ATGCTGCAGG GCGACAAGCG AGTGGCATAC 
CAGCGGGTGC CTACACATGA GGTCCTCTTC TCCCGGCGTG GAGCCAGCTA CTGCGGCAAG 
AACTGTGGGA AGCTGCAGAC GATATTTCTC AAATATCCCA TGCAGGGGGT GCCCGGAGCC 
CGGATGCCCG TGCAGATACG GGTGAAGCTC TGGTTTGGGC TTTCTGTGGA TGAGAAGGAG 
TTCAACCAGT TTGCTGAGGG AAAGCTGTCT GTCTTTGCTG AAACCTATGA GAACCAGACC 
AAACTGGCCC TCGTTGGGAA CTGGGGCACA ACAGGCCTTA CCTACCCCAA GTTTTCTGAT 
GTCGCGGGCA AGATCAAGCT CCCCAAGGAT AGCTTCCGCC CCTCAGCCGG CTGGACCTGG 
GCTGGAGATT GGTTTGTGTG TCCAGAGAAG ACGCTGCTCC ATGACACCGA CGCGGGCCAC 
CTGAGTTTCG TGGCGGAGGT GTTTGAGAAC CAGACCCGGC TCCCTGGAGG CCACTGGGTC 
TACATGAGTG ACAACTACAC GGATGTGAAT GGGGAGAAGG TGCTTCCCAA GGATGACATT 
GAGTGCCCGC TGGGCTGGAA GTGGGAAGAT GAAGAGTGGT CCACGGACCT CAATCGGGCC 
GTTGATGAGC AAGGCTGGGA GTACAGTATC ACCATCCCCC CGGACCGGAA GCCGAAGCAC 
TGGGTCCCCG CTGAGAAGAT GTACTACACG CACCGTCGGC GGCGCTGGGT CCGCCTGCGC 
AGGAGGGACC TCAGCCAGAT GGAGGCCCTG AGGAAGCACA GGCAGGCGGA GGCGGAGGGC 
GAGGGCTGGG AGTACGCCTC GCTCTTTGGC TGGAAATTCC ACCTGGAGTA CCGCAAAACG 
GATGCATTCC GTCGCCGCCG CTGGCGCCGC CGCATGGAGC CGCTGGAGAA GACGGGGCCT 
GCAGCCGTGT TTGCCCTTGA GGGGGCACTG GGTGGCGTGG TAGATGACCG GAGTGAAGAT 
TCCATGTCCG TGTCCACCTT GAGCTTTGGC GTGAACAGAC CCACGATTTC CTGCATCTTT 
GATCATGGGA ACCGCTACCA CCTGCGCTGC TATATGTACC AGGCTCGGGA CCTGCCCTCG 
ATGGACAAGG ACTCTTTTTC TGATCCCTAC GCCATCGTCT CCTTCCTGCA CCAGAGCCAG 
AAGACCGTGG TGGTGAAGAA CACTCTGAAC CCCACCTGGG ACCAGACGCT CATCTTCTAT 
GAGATTGAGA TCTTTGGAGA GCCAGCCAGC ATCGCCGAGC AGCCGCCCAG CATTGTGGTG 
GAGCTGTATG ACCACGACAC CTACGGTGCA GATGAGTTTT TGGGACGCTG CATGTGTCAG 
CCCAGTCTGG AACGGATGCC CCGGCTGGCC TGGTTCCCGC TGGTTAGGGG CAGCCAGCCG 
GCAGGCGAGC TGCTGGCCTC TTTTGAGCTC ATCCAGAGAG AGAAGCCGGC CATTCACCAT 
ATTCCTGGTT TTGAGGTGCA GGATACATCG GGAATCCTGG ACGAGCTGTG TGCACCTGCC 
TTCTTCCTCC AGGGCCTCCT CCAAACGTCG GAGGACACAG ACCTGCCCTA CCCACCGCCT 
CAGAGGGAGG CCAACATCTA CATGGTTCCC CAGAACATCA AGCCGGTTCT CCAGCGCACT 
GCCATAGAGA TCCTGGCATG GGGCCTACGG AACATGAAGA GTTACCAGCT GGCCAGTATC 
TCCTCCCCCA GCCTCGTGGT GGAGTGCGGG GGCCAGACGG TGCAGTCCTG TGTCATCAGG 
AACCTCCGGA AGAACCCCAA CTTCGACATC TGCACCCTCT TCATGGAAGT GATGCTGCCC 
AGAGAGGAGC TCTACTGTCC CCCCATCGTG ATCAAGGTCA TTGATAACCG CCAGTTTGGC 
CGCCGGCCTG TGGTGGGCCA GTGTACCATA CGCTCCCTGG AGAGCTTCAT GTGTGACCTG 
TACTCCGAGG AGAGTCCGTC CCCACAGGGC GGCCCAGATG ATGTGAGTTT ACTCAGTCCT 
GGGGAAGACG TGCTCATTGA CATCGACGAC AAGGAGCCCC TCATCCCCAT ACAGGAGGAG 
GAGTTCATCG ACTGGTGGAG CAAATTCTTT GCCTCCACAG GGGAGAGGGA GAAGTGCGGC 
TCGTACTTGG AGAAGGGCTT TGACACTCTA AAGGTCTATG ACACACAACT GGAGAACGTG 
GAGGCCTTTG GGGGCCTGTC TGACTTTTGT AACACCTTCA AGCTCTACCG GGGCAAGACT 
CAGGAGGAGA CGGAGGACCC ATCTGTTATC GGGGAGTTTA AGGGCCTCTT CAAAATCTAC 
CCCCTCCCGG AAGACCCGGC CGTCCCCATG CCCCCGAGAC AGTTCCAGCA GCTGGCTGCC 
CAGGGGCCTC AGGACTGCCT GGTCCGCATC TATATCATCC GGGCATTTGG CCTGCAGCCC 
AAGGACCCCA ATGGGAAGTG TGATCCTTAC ATCAAGATCT CCATAGGGAA GAAATCAGTG 
AGTGACCAGG ATAATTACAT TCCCTGTACC CTGGAGCCTG TGTTTGGAAA GATGTTCGAG 
CTGACCTGCA CTCTGCCTCT GGAGAAGGAC CTGAAGATCA CACTCTACGA CTATGACCTC 
CTCTCCAAAG ATGAGAAGAT CGGGGAGACA GTCATCGACC TGGAGAATAG GCTGCTGTCC 
AAGTTCGGGG CTCGCTGCGG ACTCCCACAG ACCTACTGTG TCTCTGGACC CAACCAGTGG 
AGGGACCAGC TCCGCCCCTC CCAGCTTCTC CAGCTCACCT GCCAGCAGCG CAGACTCAAG 
GCGCCCGTGT ACCTCACAGA CCGCGTACTC TTTCAGGATA AAGAATACAC CATTGAGGAA 
ATAGAGGCTG GCAGGGTCCT GAACCCACAC CTGGGCCCAG TGGAAGAGCG TCTGGCCTTG 
CACGTCCTCC AGCAGCTGGG CCTGGTCCCC GAGCATGTGG AGTCCCGGCC TCTTTACAGC 
CCCCTGCAGC CAGACATTGA GCAGGGGAAG CTGCAGATGT GGATCGATCT GTTTCCCAAG 
GCCCTGGGGC GACCTGGACC TCCCTTCAAC ATCACCCCAC GGAGAGCAAG AAGGTTTTTC 
CTGCGTTGTA TTATCTGGAA CACAAAGGAC GTGATCCTGG ACGACCTCAG CATCACGGGG 
GAGAAGATGA GTGACATTTA CGTGAAAGGT TGGATGGTCG GCTTTGAAGA GCACAAGCAG 
AAGACGGACG TGCACTACCG CTCCCTGGGA GGCGAAGGCA ACTTTAACTG GAGGTTCATT 
TTCCCCTTTG ACTACCTGCC CGCTGAGCAA GTCTGCACCG TCTCTAAGAA GGATGCTTTC 
TGGAGGCTGG ACAAGACTGA GAGCAAAATC CCAGCACAAG TGGTATTTCA GATCTGGGAC 
AATGACAAGT TCTCCTTTGA CGACTTTCTG GGCTCGTTGC AGTTGGATCT CAACCGCATG 
CCCAAGCCTG CCAAGACAGC TGAGAAGTGC TCCTTGGACC AGCTGGACGA CACTTTCCAC 
CCGGAGTGGT TTGTGTCCCT TTTTGAACAG AAAACAGTAA AGGGCTGGTG GCCCTGTGTG 
GCAGAAGACG GTGAGAAGAA GATCCTGGCG GGCAAGCTGG AAATGACCTT GGAGATCGTA 
GCAGAGAGTG AGCACGAAGA GCGGCCTGCG GGTCAAGGCC GGGATGAACC CAACATGAAC 
CCCAAGCTCG AGGACCCAAG GCGCCCCGAC ACCTCCTTCC TGTGGTTCAC CTCTCCGTAC 
AAGACCATGA AGTTCATCCT GTGGCGGCGG TTCCGGTGCG CCATCATCCT CTTCGTCGTT 
CTCGTCATCC TGCTGCTCTT CCTGGGCATC TTCCTCTATT CCTTCCCGAA CTATGCTGCC 
ATGAAGCTGG TGAAACCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_019794825.1 ) cDNA ORF clone, Ailuropoda melanoleuca(giant panda) -> XP_019650384.1 Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X8, mRNA.

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CloneID	OAc89679
Clone ID Related Accession (Same CDS sequence)	XM_019794825.1
Accession Version	XM_019794825.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-27
Organism	Ailuropoda melanoleuca(giant panda)
Product	dysferlin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003217356.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ailuropoda melanoleuca Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGTGAAGAA GGACCGGCGC 
AGTGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCAGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTCCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCGG GAGGTCCTCG CCACCCCCAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCCTC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGTGGCTG ACACAGGAGG AGAGGAGGAC 
ACTGAGGACC AGGGGCTCAC GGGAGATGAG GCAGAGCCAT TACTGGATCC GAGCGGTGCC 
CCAGGCCCTG GGGCCCCCAC CACCCCGAGG AAGCCACCTT CCCATCCGCC CCCCTACCAC 
CCCGGGAGCA AGAGAAAAAG AGGTGCTCCC ACGCCCAGAA AGCTGCTGTC GGACAAACCG 
CAGGACTTCC AGATCAGGGT GCAAGTGATC GAGGGGCGCC AGCTGCCAGG GGTGAATATC 
AAGCCTGTGG TCAAAGTCAC GGCTGCCGGG CAGACCAAGA GGACACGGAT CCACAAGGGA 
AACAGCCCAC TCTTCAACGA GACTCTCTTC TTCAATGTGT TTGACTCTCC CTCGGAGCTG 
TTTGACGAGC CCATCTTTAT CACGGTGGTA GACTCCCGTT CTCTCCGGAC AGACGCTCTC 
ATTGGGGAAT TCCGGATGGA CGTGGGTACC ATCTACAGAG AGCCCCGACA CGCCTATCTG 
AGGAAATGGC TTCTCCTCTC GGACCCTGAC GATTTCTCTG CTGGGGCCAG AGGTTACCTG 
AAAGCAAGCC TCTGTGTGCT GGGGCCCGGC GATGAAGCGC CTCTGGAGAG AAAGGACCCC 
TCTGAAGACA AGGAGGACAT CGAAAACAAT CTGCTCAGGC CAACTGGCGT GGCCCTTCGA 
GGAGCCCACT TCTGCCTGAA GGTCTTCAGA GCTGAGGACT TACCACAGAT GGATGATGCC 
GTGATGGACA ACGTGAAGCA GATCTTCGGC TTTGACAGTA ACAAGAAGAA CTTGGTGGAT 
CCCTTCATGG AAGTCAGCTT TGCGGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAAACA 
GCCAACCCTC AGTGGAACCA GAGCATCACG CTGCCCGCCA TGTTTCCCTC CATGTGTGAA 
AAAATGAGGA TTCGCGTCAT GGACTGGGAC CGCCTCACCC ACAATGACAT CGTGGCCACC 
ACCTACCTGA GTATGTCGAA AATCTCCGCC CCTGGAGGAG AAATAGAAGA GGAGCCTGCA 
GGTGTGGCCA AGCCTTCTCC AGCCATGGAC CTGGACGACC ATCTGGGCTT CCTCCCCACC 
TTCGGGCCCT GCTACGTCAA CCTCTACGGC AGCCCCAGGG AGTTCACCGG CTTCCCGGAC 
CCCTACGCAG AACTCAACAT GGGCAAGGGA GAAGGTGTGG CCTACCGAGG TCGGCTTCTG 
CTCTCCCTGG AGACCAAGCT GGTGGAGCAC AGCGACCAAA AGGTGGAGGA CCTCCCTGCG 
GATGACATCC TTCGGGTGGA GAAGTATCTC CGGAGGCGTA AGTACTCCCT CTTTGCCGCC 
TTCTACTCGG CCTCCATGCT CCAGGATGTG GATGATGCCA TCCAGTTCGA GGTCAGCATC 
GGGAACTATG GCAACAAGTT CGACACCACG TGCCTGCCGC TGGCCTCTAC CACCCAGTAC 
AGCCGGGCGG TCTTTGACGG GTGCCACTAC TACTACTTGC CCTGGGGCAA CGTGAAGCCC 
GTGGTGGTGC TGTCATCCTA CTGGGAGGAC ATCAGCCACA GAGTTGAGGC TCAGAACCAG 
CTGCTCAGGA TTGCGGACCG GCTGGAAGCT GCCCTGGAGC AAGTCCACTT GGCCGTGAAG 
GCGCAGTACA ACGCCGAGGA CGTGGACTCC CTGTTGGCTC AGCTGATGGA TGAGCTCATC 
TCAGACTGCA GCCAGCCCCT AGGCGATGTC CACGAGACAC CCTCTGCCAC CCACCTGGAC 
CAGTACCTGC ACAGGCTGCG TAGCCGTCAC CTAAGCCAGA TTGCAGAGGC GGCGCTGGCC 
CTGAAGCTCG GGCACGGCGG GCTCCCGGCC GCCCTGGAGC AGGCTGAAGA CTGGCTCCTG 
CGGCTCCGTG CCCTGGCCGA GGAGCCTCAG AACAGCCTGC CCGACATCAT CATCTGGATG 
CTGCAGGGCG ACAAGCGAGT GGCATACCAG CGGGTGCCTA CACATGAGGT CCTCTTCTCC 
CGGCGTGGAG CCAGCTACTG CGGCAAGAAC TGTGGGAAGC TGCAGACGAT ATTTCTCAAA 
TATCCCATGC AGGGGGTGCC CGGAGCCCGG ATGCCCGTGC AGATACGGGT GAAGCTCTGG 
TTTGGGCTTT CTGTGGATGA GAAGGAGTTC AACCAGTTTG CTGAGGGAAA GCTGTCTGTC 
TTTGCTGAAA CCTATGAGAA CCAGACCAAA CTGGCCCTCG TTGGGAACTG GGGCACAACA 
GGCCTTACCT ACCCCAAGTT TTCTGATGTC GCGGGCAAGA TCAAGCTCCC CAAGGATAGC 
TTCCGCCCCT CAGCCGGCTG GACCTGGGCT GGAGATTGGT TTGTGTGTCC AGAGAAGACG 
CTGCTCCATG ACACCGACGC GGGCCACCTG AGTTTCGTGG CGGAGGTGTT TGAGAACCAG 
ACCCGGCTCC CTGGAGGCCA CTGGGTCTAC ATGAGTGACA ACTACACGGA TGTGAATGGG 
GAGAAGGTGC TTCCCAAGGA TGACATTGAG TGCCCGCTGG GCTGGAAGTG GGAAGATGAA 
GAGTGGTCCA CGGACCTCAA TCGGGCCGTT GATGAGCAAG GCTGGGAGTA CAGTATCACC 
ATCCCCCCGG ACCGGAAGCC GAAGCACTGG GTCCCCGCTG AGAAGATGTA CTACACGCAC 
CGTCGGCGGC GCTGGGTCCG CCTGCGCAGG AGGGACCTCA GCCAGATGGA GGCCCTGAGG 
AAGCACAGGC AGGCGGAGGC GGAGGGCGAG GGCTGGGAGT ACGCCTCGCT CTTTGGCTGG 
AAATTCCACC TGGAGTACCG CAAAACGGAT GCATTCCGTC GCCGCCGCTG GCGCCGCCGC 
ATGGAGCCGC TGGAGAAGAC GGGGCCTGCA GCCGTGTTTG CCCTTGAGGG GGCACTGGGT 
GGCGTGGTAG ATGACCGGAG TGAAGATTCC ATGTCCGTGT CCACCTTGAG CTTTGGCGTG 
AACAGACCCA CGATTTCCTG CATCTTTGAT CATGGGAACC GCTACCACCT GCGCTGCTAT 
ATGTACCAGG CTCGGGACCT GCCCTCGATG GACAAGGACT CTTTTTCTGA TCCCTACGCC 
ATCGTCTCCT TCCTGCACCA GAGCCAGAAG ACCGTGGTGG TGAAGAACAC TCTGAACCCC 
ACCTGGGACC AGACGCTCAT CTTCTATGAG ATTGAGATCT TTGGAGAGCC AGCCAGCATC 
GCCGAGCAGC CGCCCAGCAT TGTGGTGGAG CTGTATGACC ACGACACCTA CGGTGCAGAT 
GAGTTTTTGG GACGCTGCAT GTGTCAGCCC AGTCTGGAAC GGATGCCCCG GCTGGCCTGG 
TTCCCGCTGG TTAGGGGCAG CCAGCCGGCA GGCGAGCTGC TGGCCTCTTT TGAGCTCATC 
CAGAGAGAGA AGCCGGCCAT TCACCATATT CCTGGTTTTG AGGTGCAGGA TACATCGGGA 
ATCCTGGACG AGCTGTGTGC ACCTGCCTTC TTCCTCCAGG GCCTCCTCCA AACGTCGGAG 
GACACAGACC TGCCCTACCC ACCGCCTCAG AGGGAGGCCA ACATCTACAT GGTTCCCCAG 
AACATCAAGC CGGTTCTCCA GCGCACTGCC ATAGAGATCC TGGCATGGGG CCTACGGAAC 
ATGAAGAGTT ACCAGCTGGC CAGTATCTCC TCCCCCAGCC TCGTGGTGGA GTGCGGGGGC 
CAGACGGTGC AGTCCTGTGT CATCAGGAAC CTCCGGAAGA ACCCCAACTT CGACATCTGC 
ACCCTCTTCA TGGAAGTGAT GCTGCCCAGA GAGGAGCTCT ACTGTCCCCC CATCGTGATC 
AAGGTCATTG ATAACCGCCA GTTTGGCCGC CGGCCTGTGG TGGGCCAGTG TACCATACGC 
TCCCTGGAGA GCTTCATGTG TGACCTGTAC TCCGAGGAGA GTCCGTCCCC ACAGGGCGGC 
CCAGATGATG TGAGTTTACT CAGTCCTGGG GAAGACGTGC TCATTGACAT CGACGACAAG 
GAGCCCCTCA TCCCCATACA GCTTACAGAC GGTCTGTTGG GCTCTGCCCC CACTAACATG 
GCATCCTCTC CATCCAGTCT TCATGAGGAG GAGTTCATCG ACTGGTGGAG CAAATTCTTT 
GCCTCCACAG GGGAGAGGGA GAAGTGCGGC TCGTACTTGG AGAAGGGCTT TGACACTCTA 
AAGGTCTATG ACACACAACT GGAGAACGTG GAGGCCTTTG GGGGCCTGTC TGACTTTTGT 
AACACCTTCA AGCTCTACCG GGGCAAGACT CAGGAGGAGA CGGAGGACCC ATCTGTTATC 
GGGGAGTTTA AGGGCCTCTT CAAAATCTAC CCCCTCCCGG AAGACCCGGC CGTCCCCATG 
CCCCCGAGAC AGTTCCAGCA GCTGGCTGCC CAGGGGCCTC AGGACTGCCT GGTCCGCATC 
TATATCATCC GGGCATTTGG CCTGCAGCCC AAGGACCCCA ATGGGAAGTG TGATCCTTAC 
ATCAAGATCT CCATAGGGAA GAAATCAGTG AGTGACCAGG ATAATTACAT TCCCTGTACC 
CTGGAGCCTG TGTTTGGAAA GATGTTCGAG CTGACCTGCA CTCTGCCTCT GGAGAAGGAC 
CTGAAGATCA CACTCTACGA CTATGACCTC CTCTCCAAAG ATGAGAAGAT CGGGGAGACA 
GTCATCGACC TGGAGAATAG GCTGCTGTCC AAGTTCGGGG CTCGCTGCGG ACTCCCACAG 
ACCTACTGTG TCTCTGGACC CAACCAGTGG AGGGACCAGC TCCGCCCCTC CCAGCTTCTC 
CAGCTCACCT GCCAGCAGCG CAGACTCAAG GCGCCCGTGT ACCTCACAGA CCGCGTACTC 
TTTCAGGATA AAGAATACAC CATTGAGGAA ATAGAGGCTG GCAGGGTCCT GAACCCACAC 
CTGGGCCCAG TGGAAGAGCG TCTGGCCTTG CACGTCCTCC AGCAGCTGGG CCTGGTCCCC 
GAGCATGTGG AGTCCCGGCC TCTTTACAGC CCCCTGCAGC CAGACATTGA GCAGGGGAAG 
CTGCAGATGT GGATCGATCT GTTTCCCAAG GCCCTGGGGC GACCTGGACC TCCCTTCAAC 
ATCACCCCAC GGAGAGCAAG AAGGTTTTTC CTGCGTTGTA TTATCTGGAA CACAAAGGAC 
GTGATCCTGG ACGACCTCAG CATCACGGGG GAGAAGATGA GTGACATTTA CGTGAAAGGT 
TGGATGGTCG GCTTTGAAGA GCACAAGCAG AAGACGGACG TGCACTACCG CTCCCTGGGA 
GGCGAAGGCA ACTTTAACTG GAGGTTCATT TTCCCCTTTG ACTACCTGCC CGCTGAGCAA 
GTCTGCACCG TCTCTAAGAA GGATGCTTTC TGGAGGCTGG ACAAGACTGA GAGCAAAATC 
CCAGCACAAG TGGTATTTCA GATCTGGGAC AATGACAAGT TCTCCTTTGA CGACTTTCTG 
GGCTCGTTGC AGTTGGATCT CAACCGCATG CCCAAGCCTG CCAAGACAGC TGAGAAGTGC 
TCCTTGGACC AGCTGGACGA CACTTTCCAC CCGGAGTGGT TTGTGTCCCT TTTTGAACAG 
AAAACAGTAA AGGGCTGGTG GCCCTGTGTG GCAGAAGACG GTGAGAAGAA GATCCTGGCG 
GGCAAGCTGG AAATGACCTT GGAGATCGTA GCAGAGAGTG AGCACGAAGA GCGGCCTGCG 
GGTCAAGGCC GGGATGAACC CAACATGAAC CCCAAGCTCG AGGACCCAAG GCGCCCCGAC 
ACCTCCTTCC TGTGGTTCAC CTCTCCGTAC AAGACCATGA AGTTCATCCT GTGGCGGCGG 
TTCCGGTGCG CCATCATCCT CTTCGTCGTT CTCGTCATCC TGCTGCTCTT CCTGGGCATC 
TTCCTCTATT CCTTCCCGAA CTATGCTGCC ATGAAGCTGG TGAAACCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019650384.1
CDS	150..6506
Translation	MLSCLLVRASNLPSVKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEAQVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPSASLEPSPTLPDMDMVADTGGEEDTEDQGLTGDEAEPLLDPSGAPGPGAPTTPRKPPSHPPPYHPGSKRKRGAPTPRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVMDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGVAKPSPAMDLDDHLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRLLLSLETKLVEHSDQKVEDLPADDILRVEKYLRRRKYSLFAAFYSASMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADRLEAALEQVHLAVKAQYNAEDVDSLLAQLMDELISDCSQPLGDVHETPSATHLDQYLHRLRSRHLSQIAEAALALKLGHGGLPAALEQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPTHEVLFSRRGASYCGKNCGKLQTIFLKYPMQGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVAGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDTDAGHLSFVAEVFENQTRLPGGHWVYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALRKHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSMSVSTLSFGVNRPTISCIFDHGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFLGRCMCQPSLERMPRLAWFPLVRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTSGILDELCAPAFFLQGLLQTSEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPIVIKVIDNRQFGRRPVVGQCTIRSLESFMCDLYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLTDGLLGSAPTNMASSPSSLHEEEFIDWWSKFFASTGEREKCGSYLEKGFDTLKVYDTQLENVEAFGGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAVPMPPRQFQQLAAQGPQDCLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLQLTCQQRRLKAPVYLTDRVLFQDKEYTIEEIEAGRVLNPHLGPVEERLALHVLQQLGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPAQVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEDGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFVVLVILLLFLGIFLYSFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_019794825.1
Organism	Ailuropoda melanoleuca(giant panda)
Definition	Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019794825.1

ORF Insert Sequence:

ATGCTGAGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGTGAAGAA GGACCGGCGC 
AGTGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCAGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTCCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCGG GAGGTCCTCG CCACCCCCAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCCTC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGTGGCTG ACACAGGAGG AGAGGAGGAC 
ACTGAGGACC AGGGGCTCAC GGGAGATGAG GCAGAGCCAT TACTGGATCC GAGCGGTGCC 
CCAGGCCCTG GGGCCCCCAC CACCCCGAGG AAGCCACCTT CCCATCCGCC CCCCTACCAC 
CCCGGGAGCA AGAGAAAAAG AGGTGCTCCC ACGCCCAGAA AGCTGCTGTC GGACAAACCG 
CAGGACTTCC AGATCAGGGT GCAAGTGATC GAGGGGCGCC AGCTGCCAGG GGTGAATATC 
AAGCCTGTGG TCAAAGTCAC GGCTGCCGGG CAGACCAAGA GGACACGGAT CCACAAGGGA 
AACAGCCCAC TCTTCAACGA GACTCTCTTC TTCAATGTGT TTGACTCTCC CTCGGAGCTG 
TTTGACGAGC CCATCTTTAT CACGGTGGTA GACTCCCGTT CTCTCCGGAC AGACGCTCTC 
ATTGGGGAAT TCCGGATGGA CGTGGGTACC ATCTACAGAG AGCCCCGACA CGCCTATCTG 
AGGAAATGGC TTCTCCTCTC GGACCCTGAC GATTTCTCTG CTGGGGCCAG AGGTTACCTG 
AAAGCAAGCC TCTGTGTGCT GGGGCCCGGC GATGAAGCGC CTCTGGAGAG AAAGGACCCC 
TCTGAAGACA AGGAGGACAT CGAAAACAAT CTGCTCAGGC CAACTGGCGT GGCCCTTCGA 
GGAGCCCACT TCTGCCTGAA GGTCTTCAGA GCTGAGGACT TACCACAGAT GGATGATGCC 
GTGATGGACA ACGTGAAGCA GATCTTCGGC TTTGACAGTA ACAAGAAGAA CTTGGTGGAT 
CCCTTCATGG AAGTCAGCTT TGCGGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAAACA 
GCCAACCCTC AGTGGAACCA GAGCATCACG CTGCCCGCCA TGTTTCCCTC CATGTGTGAA 
AAAATGAGGA TTCGCGTCAT GGACTGGGAC CGCCTCACCC ACAATGACAT CGTGGCCACC 
ACCTACCTGA GTATGTCGAA AATCTCCGCC CCTGGAGGAG AAATAGAAGA GGAGCCTGCA 
GGTGTGGCCA AGCCTTCTCC AGCCATGGAC CTGGACGACC ATCTGGGCTT CCTCCCCACC 
TTCGGGCCCT GCTACGTCAA CCTCTACGGC AGCCCCAGGG AGTTCACCGG CTTCCCGGAC 
CCCTACGCAG AACTCAACAT GGGCAAGGGA GAAGGTGTGG CCTACCGAGG TCGGCTTCTG 
CTCTCCCTGG AGACCAAGCT GGTGGAGCAC AGCGACCAAA AGGTGGAGGA CCTCCCTGCG 
GATGACATCC TTCGGGTGGA GAAGTATCTC CGGAGGCGTA AGTACTCCCT CTTTGCCGCC 
TTCTACTCGG CCTCCATGCT CCAGGATGTG GATGATGCCA TCCAGTTCGA GGTCAGCATC 
GGGAACTATG GCAACAAGTT CGACACCACG TGCCTGCCGC TGGCCTCTAC CACCCAGTAC 
AGCCGGGCGG TCTTTGACGG GTGCCACTAC TACTACTTGC CCTGGGGCAA CGTGAAGCCC 
GTGGTGGTGC TGTCATCCTA CTGGGAGGAC ATCAGCCACA GAGTTGAGGC TCAGAACCAG 
CTGCTCAGGA TTGCGGACCG GCTGGAAGCT GCCCTGGAGC AAGTCCACTT GGCCGTGAAG 
GCGCAGTACA ACGCCGAGGA CGTGGACTCC CTGTTGGCTC AGCTGATGGA TGAGCTCATC 
TCAGACTGCA GCCAGCCCCT AGGCGATGTC CACGAGACAC CCTCTGCCAC CCACCTGGAC 
CAGTACCTGC ACAGGCTGCG TAGCCGTCAC CTAAGCCAGA TTGCAGAGGC GGCGCTGGCC 
CTGAAGCTCG GGCACGGCGG GCTCCCGGCC GCCCTGGAGC AGGCTGAAGA CTGGCTCCTG 
CGGCTCCGTG CCCTGGCCGA GGAGCCTCAG AACAGCCTGC CCGACATCAT CATCTGGATG 
CTGCAGGGCG ACAAGCGAGT GGCATACCAG CGGGTGCCTA CACATGAGGT CCTCTTCTCC 
CGGCGTGGAG CCAGCTACTG CGGCAAGAAC TGTGGGAAGC TGCAGACGAT ATTTCTCAAA 
TATCCCATGC AGGGGGTGCC CGGAGCCCGG ATGCCCGTGC AGATACGGGT GAAGCTCTGG 
TTTGGGCTTT CTGTGGATGA GAAGGAGTTC AACCAGTTTG CTGAGGGAAA GCTGTCTGTC 
TTTGCTGAAA CCTATGAGAA CCAGACCAAA CTGGCCCTCG TTGGGAACTG GGGCACAACA 
GGCCTTACCT ACCCCAAGTT TTCTGATGTC GCGGGCAAGA TCAAGCTCCC CAAGGATAGC 
TTCCGCCCCT CAGCCGGCTG GACCTGGGCT GGAGATTGGT TTGTGTGTCC AGAGAAGACG 
CTGCTCCATG ACACCGACGC GGGCCACCTG AGTTTCGTGG CGGAGGTGTT TGAGAACCAG 
ACCCGGCTCC CTGGAGGCCA CTGGGTCTAC ATGAGTGACA ACTACACGGA TGTGAATGGG 
GAGAAGGTGC TTCCCAAGGA TGACATTGAG TGCCCGCTGG GCTGGAAGTG GGAAGATGAA 
GAGTGGTCCA CGGACCTCAA TCGGGCCGTT GATGAGCAAG GCTGGGAGTA CAGTATCACC 
ATCCCCCCGG ACCGGAAGCC GAAGCACTGG GTCCCCGCTG AGAAGATGTA CTACACGCAC 
CGTCGGCGGC GCTGGGTCCG CCTGCGCAGG AGGGACCTCA GCCAGATGGA GGCCCTGAGG 
AAGCACAGGC AGGCGGAGGC GGAGGGCGAG GGCTGGGAGT ACGCCTCGCT CTTTGGCTGG 
AAATTCCACC TGGAGTACCG CAAAACGGAT GCATTCCGTC GCCGCCGCTG GCGCCGCCGC 
ATGGAGCCGC TGGAGAAGAC GGGGCCTGCA GCCGTGTTTG CCCTTGAGGG GGCACTGGGT 
GGCGTGGTAG ATGACCGGAG TGAAGATTCC ATGTCCGTGT CCACCTTGAG CTTTGGCGTG 
AACAGACCCA CGATTTCCTG CATCTTTGAT CATGGGAACC GCTACCACCT GCGCTGCTAT 
ATGTACCAGG CTCGGGACCT GCCCTCGATG GACAAGGACT CTTTTTCTGA TCCCTACGCC 
ATCGTCTCCT TCCTGCACCA GAGCCAGAAG ACCGTGGTGG TGAAGAACAC TCTGAACCCC 
ACCTGGGACC AGACGCTCAT CTTCTATGAG ATTGAGATCT TTGGAGAGCC AGCCAGCATC 
GCCGAGCAGC CGCCCAGCAT TGTGGTGGAG CTGTATGACC ACGACACCTA CGGTGCAGAT 
GAGTTTTTGG GACGCTGCAT GTGTCAGCCC AGTCTGGAAC GGATGCCCCG GCTGGCCTGG 
TTCCCGCTGG TTAGGGGCAG CCAGCCGGCA GGCGAGCTGC TGGCCTCTTT TGAGCTCATC 
CAGAGAGAGA AGCCGGCCAT TCACCATATT CCTGGTTTTG AGGTGCAGGA TACATCGGGA 
ATCCTGGACG AGCTGTGTGC ACCTGCCTTC TTCCTCCAGG GCCTCCTCCA AACGTCGGAG 
GACACAGACC TGCCCTACCC ACCGCCTCAG AGGGAGGCCA ACATCTACAT GGTTCCCCAG 
AACATCAAGC CGGTTCTCCA GCGCACTGCC ATAGAGATCC TGGCATGGGG CCTACGGAAC 
ATGAAGAGTT ACCAGCTGGC CAGTATCTCC TCCCCCAGCC TCGTGGTGGA GTGCGGGGGC 
CAGACGGTGC AGTCCTGTGT CATCAGGAAC CTCCGGAAGA ACCCCAACTT CGACATCTGC 
ACCCTCTTCA TGGAAGTGAT GCTGCCCAGA GAGGAGCTCT ACTGTCCCCC CATCGTGATC 
AAGGTCATTG ATAACCGCCA GTTTGGCCGC CGGCCTGTGG TGGGCCAGTG TACCATACGC 
TCCCTGGAGA GCTTCATGTG TGACCTGTAC TCCGAGGAGA GTCCGTCCCC ACAGGGCGGC 
CCAGATGATG TGAGTTTACT CAGTCCTGGG GAAGACGTGC TCATTGACAT CGACGACAAG 
GAGCCCCTCA TCCCCATACA GCTTACAGAC GGTCTGTTGG GCTCTGCCCC CACTAACATG 
GCATCCTCTC CATCCAGTCT TCATGAGGAG GAGTTCATCG ACTGGTGGAG CAAATTCTTT 
GCCTCCACAG GGGAGAGGGA GAAGTGCGGC TCGTACTTGG AGAAGGGCTT TGACACTCTA 
AAGGTCTATG ACACACAACT GGAGAACGTG GAGGCCTTTG GGGGCCTGTC TGACTTTTGT 
AACACCTTCA AGCTCTACCG GGGCAAGACT CAGGAGGAGA CGGAGGACCC ATCTGTTATC 
GGGGAGTTTA AGGGCCTCTT CAAAATCTAC CCCCTCCCGG AAGACCCGGC CGTCCCCATG 
CCCCCGAGAC AGTTCCAGCA GCTGGCTGCC CAGGGGCCTC AGGACTGCCT GGTCCGCATC 
TATATCATCC GGGCATTTGG CCTGCAGCCC AAGGACCCCA ATGGGAAGTG TGATCCTTAC 
ATCAAGATCT CCATAGGGAA GAAATCAGTG AGTGACCAGG ATAATTACAT TCCCTGTACC 
CTGGAGCCTG TGTTTGGAAA GATGTTCGAG CTGACCTGCA CTCTGCCTCT GGAGAAGGAC 
CTGAAGATCA CACTCTACGA CTATGACCTC CTCTCCAAAG ATGAGAAGAT CGGGGAGACA 
GTCATCGACC TGGAGAATAG GCTGCTGTCC AAGTTCGGGG CTCGCTGCGG ACTCCCACAG 
ACCTACTGTG TCTCTGGACC CAACCAGTGG AGGGACCAGC TCCGCCCCTC CCAGCTTCTC 
CAGCTCACCT GCCAGCAGCG CAGACTCAAG GCGCCCGTGT ACCTCACAGA CCGCGTACTC 
TTTCAGGATA AAGAATACAC CATTGAGGAA ATAGAGGCTG GCAGGGTCCT GAACCCACAC 
CTGGGCCCAG TGGAAGAGCG TCTGGCCTTG CACGTCCTCC AGCAGCTGGG CCTGGTCCCC 
GAGCATGTGG AGTCCCGGCC TCTTTACAGC CCCCTGCAGC CAGACATTGA GCAGGGGAAG 
CTGCAGATGT GGATCGATCT GTTTCCCAAG GCCCTGGGGC GACCTGGACC TCCCTTCAAC 
ATCACCCCAC GGAGAGCAAG AAGGTTTTTC CTGCGTTGTA TTATCTGGAA CACAAAGGAC 
GTGATCCTGG ACGACCTCAG CATCACGGGG GAGAAGATGA GTGACATTTA CGTGAAAGGT 
TGGATGGTCG GCTTTGAAGA GCACAAGCAG AAGACGGACG TGCACTACCG CTCCCTGGGA 
GGCGAAGGCA ACTTTAACTG GAGGTTCATT TTCCCCTTTG ACTACCTGCC CGCTGAGCAA 
GTCTGCACCG TCTCTAAGAA GGATGCTTTC TGGAGGCTGG ACAAGACTGA GAGCAAAATC 
CCAGCACAAG TGGTATTTCA GATCTGGGAC AATGACAAGT TCTCCTTTGA CGACTTTCTG 
GGCTCGTTGC AGTTGGATCT CAACCGCATG CCCAAGCCTG CCAAGACAGC TGAGAAGTGC 
TCCTTGGACC AGCTGGACGA CACTTTCCAC CCGGAGTGGT TTGTGTCCCT TTTTGAACAG 
AAAACAGTAA AGGGCTGGTG GCCCTGTGTG GCAGAAGACG GTGAGAAGAA GATCCTGGCG 
GGCAAGCTGG AAATGACCTT GGAGATCGTA GCAGAGAGTG AGCACGAAGA GCGGCCTGCG 
GGTCAAGGCC GGGATGAACC CAACATGAAC CCCAAGCTCG AGGACCCAAG GCGCCCCGAC 
ACCTCCTTCC TGTGGTTCAC CTCTCCGTAC AAGACCATGA AGTTCATCCT GTGGCGGCGG 
TTCCGGTGCG CCATCATCCT CTTCGTCGTT CTCGTCATCC TGCTGCTCTT CCTGGGCATC 
TTCCTCTATT CCTTCCCGAA CTATGCTGCC ATGAAGCTGG TGAAACCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_019794826.1 ) cDNA ORF clone, Ailuropoda melanoleuca(giant panda) -> XP_019650385.1 Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X9, mRNA.

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CloneID	OAc89680
Clone ID Related Accession (Same CDS sequence)	XM_019794826.1
Accession Version	XM_019794826.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-27
Organism	Ailuropoda melanoleuca(giant panda)
Product	dysferlin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003217356.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ailuropoda melanoleuca Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTATGCCGAG AATGTCCACA CCCCGGACAC CGACATCAGC 
GATGCCTACT GCTCTGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CAGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGAGCTCCG AGCTCCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
TTAGGGGAAG CCCAAGTCCC ACTCCGGGAG GTCCTCGCCA CCCCCAGTCT GTCTGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CGCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCCTCTGC ATCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATATG GTGGCTGACA CAGGAGGAGA GGAGGACACT 
GAGGACCAGG GGCTCACGGG AGATGAGGCA GAGCCATTAC TGGATCCGAG CGGTGCCCCA 
GGCCCTGGGG CCCCCACCAC CCCGAGGAAG CCACCTTCCC ATCCGCCCCC CTACCACCCC 
GGGAGCAAGA GAAAAAGAGG TGCTCCCACG CCCAGAAAGC TGCTGTCGGA CAAACCGCAG 
GACTTCCAGA TCAGGGTGCA AGTGATCGAG GGGCGCCAGC TGCCAGGGGT GAATATCAAG 
CCTGTGGTCA AAGTCACGGC TGCCGGGCAG ACCAAGAGGA CACGGATCCA CAAGGGAAAC 
AGCCCACTCT TCAACGAGAC TCTCTTCTTC AATGTGTTTG ACTCTCCCTC GGAGCTGTTT 
GACGAGCCCA TCTTTATCAC GGTGGTAGAC TCCCGTTCTC TCCGGACAGA CGCTCTCATT 
GGGGAATTCC GGATGGACGT GGGTACCATC TACAGAGAGC CCCGACACGC CTATCTGAGG 
AAATGGCTTC TCCTCTCGGA CCCTGACGAT TTCTCTGCTG GGGCCAGAGG TTACCTGAAA 
GCAAGCCTCT GTGTGCTGGG GCCCGGCGAT GAAGCGCCTC TGGAGAGAAA GGACCCCTCT 
GAAGACAAGG AGGACATCGA AAACAATCTG CTCAGGCCAA CTGGCGTGGC CCTTCGAGGA 
GCCCACTTCT GCCTGAAGGT CTTCAGAGCT GAGGACTTAC CACAGATGGA TGATGCCGTG 
ATGGACAACG TGAAGCAGAT CTTCGGCTTT GACAGTAACA AGAAGAACTT GGTGGATCCC 
TTCATGGAAG TCAGCTTTGC GGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAAACAGCC 
AACCCTCAGT GGAACCAGAG CATCACGCTG CCCGCCATGT TTCCCTCCAT GTGTGAAAAA 
ATGAGGATTC GCGTCATGGA CTGGGACCGC CTCACCCACA ATGACATCGT GGCCACCACC 
TACCTGAGTA TGTCGAAAAT CTCCGCCCCT GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT 
GTGGCCAAGC CTTCTCCAGC CATGGACCTG GACGACCATC TGGGCTTCCT CCCCACCTTC 
GGGCCCTGCT ACGTCAACCT CTACGGCAGC CCCAGGGAGT TCACCGGCTT CCCGGACCCC 
TACGCAGAAC TCAACATGGG CAAGGGAGAA GGTGTGGCCT ACCGAGGTCG GCTTCTGCTC 
TCCCTGGAGA CCAAGCTGGT GGAGCACAGC GACCAAAAGG TGGAGGACCT CCCTGCGGAT 
GACATCCTTC GGGTGGAGAA GTATCTCCGG AGGCGTAAGT ACTCCCTCTT TGCCGCCTTC 
TACTCGGCCT CCATGCTCCA GGATGTGGAT GATGCCATCC AGTTCGAGGT CAGCATCGGG 
AACTATGGCA ACAAGTTCGA CACCACGTGC CTGCCGCTGG CCTCTACCAC CCAGTACAGC 
CGGGCGGTCT TTGACGGGTG CCACTACTAC TACTTGCCCT GGGGCAACGT GAAGCCCGTG 
GTGGTGCTGT CATCCTACTG GGAGGACATC AGCCACAGAG TTGAGGCTCA GAACCAGCTG 
CTCAGGATTG CGGACCGGCT GGAAGCTGCC CTGGAGCAAG TCCACTTGGC CGTGAAGGCG 
CAGTACAACG CCGAGGACGT GGACTCCCTG TTGGCTCAGC TGATGGATGA GCTCATCTCA 
GACTGCAGCC AGCCCCTAGG CGATGTCCAC GAGACACCCT CTGCCACCCA CCTGGACCAG 
TACCTGCACA GGCTGCGTAG CCGTCACCTA AGCCAGATTG CAGAGGCGGC GCTGGCCCTG 
AAGCTCGGGC ACGGCGGGCT CCCGGCCGCC CTGGAGCAGG CTGAAGACTG GCTCCTGCGG 
CTCCGTGCCC TGGCCGAGGA GCCTCAGAAC AGCCTGCCCG ACATCATCAT CTGGATGCTG 
CAGGGCGACA AGCGAGTGGC ATACCAGCGG GTGCCTACAC ATGAGGTCCT CTTCTCCCGG 
CGTGGAGCCA GCTACTGCGG CAAGAACTGT GGGAAGCTGC AGACGATATT TCTCAAATAT 
CCCATGCAGG GGGTGCCCGG AGCCCGGATG CCCGTGCAGA TACGGGTGAA GCTCTGGTTT 
GGGCTTTCTG TGGATGAGAA GGAGTTCAAC CAGTTTGCTG AGGGAAAGCT GTCTGTCTTT 
GCTGAAACCT ATGAGAACCA GACCAAACTG GCCCTCGTTG GGAACTGGGG CACAACAGGC 
CTTACCTACC CCAAGTTTTC TGATGTCGCG GGCAAGATCA AGCTCCCCAA GGATAGCTTC 
CGCCCCTCAG CCGGCTGGAC CTGGGCTGGA GATTGGTTTG TGTGTCCAGA GAAGACGCTG 
CTCCATGACA CCGACGCGGG CCACCTGAGT TTCGTGGCGG AGGTGTTTGA GAACCAGACC 
CGGCTCCCTG GAGGCCACTG GGTCTACATG AGTGACAACT ACACGGATGT GAATGGGGAG 
AAGGTGCTTC CCAAGGATGA CATTGAGTGC CCGCTGGGCT GGAAGTGGGA AGATGAAGAG 
TGGTCCACGG ACCTCAATCG GGCCGTTGAT GAGCAAGGCT GGGAGTACAG TATCACCATC 
CCCCCGGACC GGAAGCCGAA GCACTGGGTC CCCGCTGAGA AGATGTACTA CACGCACCGT 
CGGCGGCGCT GGGTCCGCCT GCGCAGGAGG GACCTCAGCC AGATGGAGGC CCTGAGGAAG 
CACAGGCAGG CGGAGGCGGA GGGCGAGGGC TGGGAGTACG CCTCGCTCTT TGGCTGGAAA 
TTCCACCTGG AGTACCGCAA AACGGATGCA TTCCGTCGCC GCCGCTGGCG CCGCCGCATG 
GAGCCGCTGG AGAAGACGGG GCCTGCAGCC GTGTTTGCCC TTGAGGGGGC ACTGGGTGGC 
GTGGTAGATG ACCGGAGTGA AGATTCCATG TCCGTGTCCA CCTTGAGCTT TGGCGTGAAC 
AGACCCACGA TTTCCTGCAT CTTTGATCAT GGGAACCGCT ACCACCTGCG CTGCTATATG 
TACCAGGCTC GGGACCTGCC CTCGATGGAC AAGGACTCTT TTTCTGATCC CTACGCCATC 
GTCTCCTTCC TGCACCAGAG CCAGAAGACC GTGGTGGTGA AGAACACTCT GAACCCCACC 
TGGGACCAGA CGCTCATCTT CTATGAGATT GAGATCTTTG GAGAGCCAGC CAGCATCGCC 
GAGCAGCCGC CCAGCATTGT GGTGGAGCTG TATGACCACG ACACCTACGG TGCAGATGAG 
TTTTTGGGAC GCTGCATGTG TCAGCCCAGT CTGGAACGGA TGCCCCGGCT GGCCTGGTTC 
CCGCTGGTTA GGGGCAGCCA GCCGGCAGGC GAGCTGCTGG CCTCTTTTGA GCTCATCCAG 
AGAGAGAAGC CGGCCATTCA CCATATTCCT GGTTTTGAGG TGCAGGATAC ATCGGGAATC 
CTGGACGAGC TGTGTGCACC TGCCTTCTTC CTCCAGGGCC TCCTCCAAAC GTCGGAGGAC 
ACAGACCTGC CCTACCCACC GCCTCAGAGG GAGGCCAACA TCTACATGGT TCCCCAGAAC 
ATCAAGCCGG TTCTCCAGCG CACTGCCATA GAGATCCTGG CATGGGGCCT ACGGAACATG 
AAGAGTTACC AGCTGGCCAG TATCTCCTCC CCCAGCCTCG TGGTGGAGTG CGGGGGCCAG 
ACGGTGCAGT CCTGTGTCAT CAGGAACCTC CGGAAGAACC CCAACTTCGA CATCTGCACC 
CTCTTCATGG AAGTGATGCT GCCCAGAGAG GAGCTCTACT GTCCCCCCAT CGTGATCAAG 
GTCATTGATA ACCGCCAGTT TGGCCGCCGG CCTGTGGTGG GCCAGTGTAC CATACGCTCC 
CTGGAGAGCT TCATGTGTGA CCTGTACTCC GAGGAGAGTC CGTCCCCACA GGGCGGCCCA 
GATGATGTGA GTTTACTCAG TCCTGGGGAA GACGTGCTCA TTGACATCGA CGACAAGGAG 
CCCCTCATCC CCATACAGCT TACAGACGGT CTGTTGGGCT CTGCCCCCAC TAACATGGCA 
TCCTCTCCAT CCAGTCTTCA TGAGGAGGAG TTCATCGACT GGTGGAGCAA ATTCTTTGCC 
TCCACAGGGG AGAGGGAGAA GTGCGGCTCG TACTTGGAGA AGGGCTTTGA CACTCTAAAG 
GTCTATGACA CACAACTGGA GAACGTGGAG GCCTTTGGGG GCCTGTCTGA CTTTTGTAAC 
ACCTTCAAGC TCTACCGGGG CAAGACTCAG GAGGAGACGG AGGACCCATC TGTTATCGGG 
GAGTTTAAGG GCCTCTTCAA AATCTACCCC CTCCCGGAAG ACCCGGCCGT CCCCATGCCC 
CCGAGACAGT TCCAGCAGCT GGCTGCCCAG GGGCCTCAGG ACTGCCTGGT CCGCATCTAT 
ATCATCCGGG CATTTGGCCT GCAGCCCAAG GACCCCAATG GGAAGTGTGA TCCTTACATC 
AAGATCTCCA TAGGGAAGAA ATCAGTGAGT GACCAGGATA ATTACATTCC CTGTACCCTG 
GAGCCTGTGT TTGGAAAGAT GTTCGAGCTG ACCTGCACTC TGCCTCTGGA GAAGGACCTG 
AAGATCACAC TCTACGACTA TGACCTCCTC TCCAAAGATG AGAAGATCGG GGAGACAGTC 
ATCGACCTGG AGAATAGGCT GCTGTCCAAG TTCGGGGCTC GCTGCGGACT CCCACAGACC 
TACTGTGTCT CTGGACCCAA CCAGTGGAGG GACCAGCTCC GCCCCTCCCA GCTTCTCCAG 
CTCACCTGCC AGCAGCGCAG ACTCAAGGCG CCCGTGTACC TCACAGACCG CGTACTCTTT 
CAGGATAAAG AATACACCAT TGAGGAAATA GAGGCTGGCA GGGTCCTGAA CCCACACCTG 
GGCCCAGTGG AAGAGCGTCT GGCCTTGCAC GTCCTCCAGC AGCTGGGCCT GGTCCCCGAG 
CATGTGGAGT CCCGGCCTCT TTACAGCCCC CTGCAGCCAG ACATTGAGCA GGGGAAGCTG 
CAGATGTGGA TCGATCTGTT TCCCAAGGCC CTGGGGCGAC CTGGACCTCC CTTCAACATC 
ACCCCACGGA GAGCAAGAAG GTTTTTCCTG CGTTGTATTA TCTGGAACAC AAAGGACGTG 
ATCCTGGACG ACCTCAGCAT CACGGGGGAG AAGATGAGTG ACATTTACGT GAAAGGTTGG 
ATGGTCGGCT TTGAAGAGCA CAAGCAGAAG ACGGACGTGC ACTACCGCTC CCTGGGAGGC 
GAAGGCAACT TTAACTGGAG GTTCATTTTC CCCTTTGACT ACCTGCCCGC TGAGCAAGTC 
TGCACCGTCT CTAAGAAGGA TGCTTTCTGG AGGCTGGACA AGACTGAGAG CAAAATCCCA 
GCACAAGTGG TATTTCAGAT CTGGGACAAT GACAAGTTCT CCTTTGACGA CTTTCTGGGC 
TCGTTGCAGT TGGATCTCAA CCGCATGCCC AAGCCTGCCA AGACAGCTGA GAAGTGCTCC 
TTGGACCAGC TGGACGACAC TTTCCACCCG GAGTGGTTTG TGTCCCTTTT TGAACAGAAA 
ACAGTAAAGG GCTGGTGGCC CTGTGTGGCA GAAGACGGTG AGAAGAAGAT CCTGGCGGGC 
AAGCTGGAAA TGACCTTGGA GATCGTAGCA GAGAGTGAGC ACGAAGAGCG GCCTGCGGGT 
CAAGGCCGGG ATGAACCCAA CATGAACCCC AAGCTCGAGG ACCCAAGGCG CCCCGACACC 
TCCTTCCTGT GGTTCACCTC TCCGTACAAG ACCATGAAGT TCATCCTGTG GCGGCGGTTC 
CGGTGCGCCA TCATCCTCTT CGTCGTTCTC GTCATCCTGC TGCTCTTCCT GGGCATCTTC 
CTCTATTCCT TCCCGAACTA TGCTGCCATG AAGCTGGTGA AACCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019650385.1
CDS	487..6840
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEAQVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPSASLEPSPTLPDMDMVADTGGEEDTEDQGLTGDEAEPLLDPSGAPGPGAPTTPRKPPSHPPPYHPGSKRKRGAPTPRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVMDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGVAKPSPAMDLDDHLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRLLLSLETKLVEHSDQKVEDLPADDILRVEKYLRRRKYSLFAAFYSASMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADRLEAALEQVHLAVKAQYNAEDVDSLLAQLMDELISDCSQPLGDVHETPSATHLDQYLHRLRSRHLSQIAEAALALKLGHGGLPAALEQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPTHEVLFSRRGASYCGKNCGKLQTIFLKYPMQGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVAGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDTDAGHLSFVAEVFENQTRLPGGHWVYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALRKHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSMSVSTLSFGVNRPTISCIFDHGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFLGRCMCQPSLERMPRLAWFPLVRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTSGILDELCAPAFFLQGLLQTSEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPIVIKVIDNRQFGRRPVVGQCTIRSLESFMCDLYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLTDGLLGSAPTNMASSPSSLHEEEFIDWWSKFFASTGEREKCGSYLEKGFDTLKVYDTQLENVEAFGGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAVPMPPRQFQQLAAQGPQDCLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLQLTCQQRRLKAPVYLTDRVLFQDKEYTIEEIEAGRVLNPHLGPVEERLALHVLQQLGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPAQVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEDGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFVVLVILLLFLGIFLYSFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_019794826.1
Organism	Ailuropoda melanoleuca(giant panda)
Definition	Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019794826.1

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCCGAG AATGTCCACA CCCCGGACAC CGACATCAGC 
GATGCCTACT GCTCTGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CAGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGAGCTCCG AGCTCCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
TTAGGGGAAG CCCAAGTCCC ACTCCGGGAG GTCCTCGCCA CCCCCAGTCT GTCTGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CGCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCCTCTGC ATCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATATG GTGGCTGACA CAGGAGGAGA GGAGGACACT 
GAGGACCAGG GGCTCACGGG AGATGAGGCA GAGCCATTAC TGGATCCGAG CGGTGCCCCA 
GGCCCTGGGG CCCCCACCAC CCCGAGGAAG CCACCTTCCC ATCCGCCCCC CTACCACCCC 
GGGAGCAAGA GAAAAAGAGG TGCTCCCACG CCCAGAAAGC TGCTGTCGGA CAAACCGCAG 
GACTTCCAGA TCAGGGTGCA AGTGATCGAG GGGCGCCAGC TGCCAGGGGT GAATATCAAG 
CCTGTGGTCA AAGTCACGGC TGCCGGGCAG ACCAAGAGGA CACGGATCCA CAAGGGAAAC 
AGCCCACTCT TCAACGAGAC TCTCTTCTTC AATGTGTTTG ACTCTCCCTC GGAGCTGTTT 
GACGAGCCCA TCTTTATCAC GGTGGTAGAC TCCCGTTCTC TCCGGACAGA CGCTCTCATT 
GGGGAATTCC GGATGGACGT GGGTACCATC TACAGAGAGC CCCGACACGC CTATCTGAGG 
AAATGGCTTC TCCTCTCGGA CCCTGACGAT TTCTCTGCTG GGGCCAGAGG TTACCTGAAA 
GCAAGCCTCT GTGTGCTGGG GCCCGGCGAT GAAGCGCCTC TGGAGAGAAA GGACCCCTCT 
GAAGACAAGG AGGACATCGA AAACAATCTG CTCAGGCCAA CTGGCGTGGC CCTTCGAGGA 
GCCCACTTCT GCCTGAAGGT CTTCAGAGCT GAGGACTTAC CACAGATGGA TGATGCCGTG 
ATGGACAACG TGAAGCAGAT CTTCGGCTTT GACAGTAACA AGAAGAACTT GGTGGATCCC 
TTCATGGAAG TCAGCTTTGC GGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAAACAGCC 
AACCCTCAGT GGAACCAGAG CATCACGCTG CCCGCCATGT TTCCCTCCAT GTGTGAAAAA 
ATGAGGATTC GCGTCATGGA CTGGGACCGC CTCACCCACA ATGACATCGT GGCCACCACC 
TACCTGAGTA TGTCGAAAAT CTCCGCCCCT GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT 
GTGGCCAAGC CTTCTCCAGC CATGGACCTG GACGACCATC TGGGCTTCCT CCCCACCTTC 
GGGCCCTGCT ACGTCAACCT CTACGGCAGC CCCAGGGAGT TCACCGGCTT CCCGGACCCC 
TACGCAGAAC TCAACATGGG CAAGGGAGAA GGTGTGGCCT ACCGAGGTCG GCTTCTGCTC 
TCCCTGGAGA CCAAGCTGGT GGAGCACAGC GACCAAAAGG TGGAGGACCT CCCTGCGGAT 
GACATCCTTC GGGTGGAGAA GTATCTCCGG AGGCGTAAGT ACTCCCTCTT TGCCGCCTTC 
TACTCGGCCT CCATGCTCCA GGATGTGGAT GATGCCATCC AGTTCGAGGT CAGCATCGGG 
AACTATGGCA ACAAGTTCGA CACCACGTGC CTGCCGCTGG CCTCTACCAC CCAGTACAGC 
CGGGCGGTCT TTGACGGGTG CCACTACTAC TACTTGCCCT GGGGCAACGT GAAGCCCGTG 
GTGGTGCTGT CATCCTACTG GGAGGACATC AGCCACAGAG TTGAGGCTCA GAACCAGCTG 
CTCAGGATTG CGGACCGGCT GGAAGCTGCC CTGGAGCAAG TCCACTTGGC CGTGAAGGCG 
CAGTACAACG CCGAGGACGT GGACTCCCTG TTGGCTCAGC TGATGGATGA GCTCATCTCA 
GACTGCAGCC AGCCCCTAGG CGATGTCCAC GAGACACCCT CTGCCACCCA CCTGGACCAG 
TACCTGCACA GGCTGCGTAG CCGTCACCTA AGCCAGATTG CAGAGGCGGC GCTGGCCCTG 
AAGCTCGGGC ACGGCGGGCT CCCGGCCGCC CTGGAGCAGG CTGAAGACTG GCTCCTGCGG 
CTCCGTGCCC TGGCCGAGGA GCCTCAGAAC AGCCTGCCCG ACATCATCAT CTGGATGCTG 
CAGGGCGACA AGCGAGTGGC ATACCAGCGG GTGCCTACAC ATGAGGTCCT CTTCTCCCGG 
CGTGGAGCCA GCTACTGCGG CAAGAACTGT GGGAAGCTGC AGACGATATT TCTCAAATAT 
CCCATGCAGG GGGTGCCCGG AGCCCGGATG CCCGTGCAGA TACGGGTGAA GCTCTGGTTT 
GGGCTTTCTG TGGATGAGAA GGAGTTCAAC CAGTTTGCTG AGGGAAAGCT GTCTGTCTTT 
GCTGAAACCT ATGAGAACCA GACCAAACTG GCCCTCGTTG GGAACTGGGG CACAACAGGC 
CTTACCTACC CCAAGTTTTC TGATGTCGCG GGCAAGATCA AGCTCCCCAA GGATAGCTTC 
CGCCCCTCAG CCGGCTGGAC CTGGGCTGGA GATTGGTTTG TGTGTCCAGA GAAGACGCTG 
CTCCATGACA CCGACGCGGG CCACCTGAGT TTCGTGGCGG AGGTGTTTGA GAACCAGACC 
CGGCTCCCTG GAGGCCACTG GGTCTACATG AGTGACAACT ACACGGATGT GAATGGGGAG 
AAGGTGCTTC CCAAGGATGA CATTGAGTGC CCGCTGGGCT GGAAGTGGGA AGATGAAGAG 
TGGTCCACGG ACCTCAATCG GGCCGTTGAT GAGCAAGGCT GGGAGTACAG TATCACCATC 
CCCCCGGACC GGAAGCCGAA GCACTGGGTC CCCGCTGAGA AGATGTACTA CACGCACCGT 
CGGCGGCGCT GGGTCCGCCT GCGCAGGAGG GACCTCAGCC AGATGGAGGC CCTGAGGAAG 
CACAGGCAGG CGGAGGCGGA GGGCGAGGGC TGGGAGTACG CCTCGCTCTT TGGCTGGAAA 
TTCCACCTGG AGTACCGCAA AACGGATGCA TTCCGTCGCC GCCGCTGGCG CCGCCGCATG 
GAGCCGCTGG AGAAGACGGG GCCTGCAGCC GTGTTTGCCC TTGAGGGGGC ACTGGGTGGC 
GTGGTAGATG ACCGGAGTGA AGATTCCATG TCCGTGTCCA CCTTGAGCTT TGGCGTGAAC 
AGACCCACGA TTTCCTGCAT CTTTGATCAT GGGAACCGCT ACCACCTGCG CTGCTATATG 
TACCAGGCTC GGGACCTGCC CTCGATGGAC AAGGACTCTT TTTCTGATCC CTACGCCATC 
GTCTCCTTCC TGCACCAGAG CCAGAAGACC GTGGTGGTGA AGAACACTCT GAACCCCACC 
TGGGACCAGA CGCTCATCTT CTATGAGATT GAGATCTTTG GAGAGCCAGC CAGCATCGCC 
GAGCAGCCGC CCAGCATTGT GGTGGAGCTG TATGACCACG ACACCTACGG TGCAGATGAG 
TTTTTGGGAC GCTGCATGTG TCAGCCCAGT CTGGAACGGA TGCCCCGGCT GGCCTGGTTC 
CCGCTGGTTA GGGGCAGCCA GCCGGCAGGC GAGCTGCTGG CCTCTTTTGA GCTCATCCAG 
AGAGAGAAGC CGGCCATTCA CCATATTCCT GGTTTTGAGG TGCAGGATAC ATCGGGAATC 
CTGGACGAGC TGTGTGCACC TGCCTTCTTC CTCCAGGGCC TCCTCCAAAC GTCGGAGGAC 
ACAGACCTGC CCTACCCACC GCCTCAGAGG GAGGCCAACA TCTACATGGT TCCCCAGAAC 
ATCAAGCCGG TTCTCCAGCG CACTGCCATA GAGATCCTGG CATGGGGCCT ACGGAACATG 
AAGAGTTACC AGCTGGCCAG TATCTCCTCC CCCAGCCTCG TGGTGGAGTG CGGGGGCCAG 
ACGGTGCAGT CCTGTGTCAT CAGGAACCTC CGGAAGAACC CCAACTTCGA CATCTGCACC 
CTCTTCATGG AAGTGATGCT GCCCAGAGAG GAGCTCTACT GTCCCCCCAT CGTGATCAAG 
GTCATTGATA ACCGCCAGTT TGGCCGCCGG CCTGTGGTGG GCCAGTGTAC CATACGCTCC 
CTGGAGAGCT TCATGTGTGA CCTGTACTCC GAGGAGAGTC CGTCCCCACA GGGCGGCCCA 
GATGATGTGA GTTTACTCAG TCCTGGGGAA GACGTGCTCA TTGACATCGA CGACAAGGAG 
CCCCTCATCC CCATACAGCT TACAGACGGT CTGTTGGGCT CTGCCCCCAC TAACATGGCA 
TCCTCTCCAT CCAGTCTTCA TGAGGAGGAG TTCATCGACT GGTGGAGCAA ATTCTTTGCC 
TCCACAGGGG AGAGGGAGAA GTGCGGCTCG TACTTGGAGA AGGGCTTTGA CACTCTAAAG 
GTCTATGACA CACAACTGGA GAACGTGGAG GCCTTTGGGG GCCTGTCTGA CTTTTGTAAC 
ACCTTCAAGC TCTACCGGGG CAAGACTCAG GAGGAGACGG AGGACCCATC TGTTATCGGG 
GAGTTTAAGG GCCTCTTCAA AATCTACCCC CTCCCGGAAG ACCCGGCCGT CCCCATGCCC 
CCGAGACAGT TCCAGCAGCT GGCTGCCCAG GGGCCTCAGG ACTGCCTGGT CCGCATCTAT 
ATCATCCGGG CATTTGGCCT GCAGCCCAAG GACCCCAATG GGAAGTGTGA TCCTTACATC 
AAGATCTCCA TAGGGAAGAA ATCAGTGAGT GACCAGGATA ATTACATTCC CTGTACCCTG 
GAGCCTGTGT TTGGAAAGAT GTTCGAGCTG ACCTGCACTC TGCCTCTGGA GAAGGACCTG 
AAGATCACAC TCTACGACTA TGACCTCCTC TCCAAAGATG AGAAGATCGG GGAGACAGTC 
ATCGACCTGG AGAATAGGCT GCTGTCCAAG TTCGGGGCTC GCTGCGGACT CCCACAGACC 
TACTGTGTCT CTGGACCCAA CCAGTGGAGG GACCAGCTCC GCCCCTCCCA GCTTCTCCAG 
CTCACCTGCC AGCAGCGCAG ACTCAAGGCG CCCGTGTACC TCACAGACCG CGTACTCTTT 
CAGGATAAAG AATACACCAT TGAGGAAATA GAGGCTGGCA GGGTCCTGAA CCCACACCTG 
GGCCCAGTGG AAGAGCGTCT GGCCTTGCAC GTCCTCCAGC AGCTGGGCCT GGTCCCCGAG 
CATGTGGAGT CCCGGCCTCT TTACAGCCCC CTGCAGCCAG ACATTGAGCA GGGGAAGCTG 
CAGATGTGGA TCGATCTGTT TCCCAAGGCC CTGGGGCGAC CTGGACCTCC CTTCAACATC 
ACCCCACGGA GAGCAAGAAG GTTTTTCCTG CGTTGTATTA TCTGGAACAC AAAGGACGTG 
ATCCTGGACG ACCTCAGCAT CACGGGGGAG AAGATGAGTG ACATTTACGT GAAAGGTTGG 
ATGGTCGGCT TTGAAGAGCA CAAGCAGAAG ACGGACGTGC ACTACCGCTC CCTGGGAGGC 
GAAGGCAACT TTAACTGGAG GTTCATTTTC CCCTTTGACT ACCTGCCCGC TGAGCAAGTC 
TGCACCGTCT CTAAGAAGGA TGCTTTCTGG AGGCTGGACA AGACTGAGAG CAAAATCCCA 
GCACAAGTGG TATTTCAGAT CTGGGACAAT GACAAGTTCT CCTTTGACGA CTTTCTGGGC 
TCGTTGCAGT TGGATCTCAA CCGCATGCCC AAGCCTGCCA AGACAGCTGA GAAGTGCTCC 
TTGGACCAGC TGGACGACAC TTTCCACCCG GAGTGGTTTG TGTCCCTTTT TGAACAGAAA 
ACAGTAAAGG GCTGGTGGCC CTGTGTGGCA GAAGACGGTG AGAAGAAGAT CCTGGCGGGC 
AAGCTGGAAA TGACCTTGGA GATCGTAGCA GAGAGTGAGC ACGAAGAGCG GCCTGCGGGT 
CAAGGCCGGG ATGAACCCAA CATGAACCCC AAGCTCGAGG ACCCAAGGCG CCCCGACACC 
TCCTTCCTGT GGTTCACCTC TCCGTACAAG ACCATGAAGT TCATCCTGTG GCGGCGGTTC 
CGGTGCGCCA TCATCCTCTT CGTCGTTCTC GTCATCCTGC TGCTCTTCCT GGGCATCTTC 
CTCTATTCCT TCCCGAACTA TGCTGCCATG AAGCTGGTGA AACCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_019794827.1 ) cDNA ORF clone, Ailuropoda melanoleuca(giant panda) -> XP_019650386.1 Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X10, mRNA.

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CloneID	OAc89681
Clone ID Related Accession (Same CDS sequence)	XM_019794827.1
Accession Version	XM_019794827.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6345bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-27
Organism	Ailuropoda melanoleuca(giant panda)
Product	dysferlin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003217356.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ailuropoda melanoleuca Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGTGAAGAA GGACCGGCGC 
AGTGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCAGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTCCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCGG GAGGTCCTCG CCACCCCCAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCCTC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGTGGCTG GTGGGGGACA GAGCCGGGCT 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACGGGAGAT 
GAGGCAGAGC CATTACTGGA TCCGAGCGGT GCCCCAGGCC CTGGGGCCCC CACCACCCCG 
AGGAAGCCAC CTTCCCATCC GCCCCCCTAC CACCCCGGGA GCAAGAGAAA AAGAGGTGCT 
CCCACGCCCA GAAAGCTGCT GTCGGACAAA CCGCAGGACT TCCAGATCAG GGTGCAAGTG 
ATCGAGGGGC GCCAGCTGCC AGGGGTGAAT ATCAAGCCTG TGGTCAAAGT CACGGCTGCC 
GGGCAGACCA AGAGGACACG GATCCACAAG GGAAACAGCC CACTCTTCAA CGAGACTCTC 
TTCTTCAATG TGTTTGACTC TCCCTCGGAG CTGTTTGACG AGCCCATCTT TATCACGGTG 
GTAGACTCCC GTTCTCTCCG GACAGACGCT CTCATTGGGG AATTCCGGAT GGACGTGGGT 
ACCATCTACA GAGAGCCCCG ACACGCCTAT CTGAGGAAAT GGCTTCTCCT CTCGGACCCT 
GACGATTTCT CTGCTGGGGC CAGAGGTTAC CTGAAAGCAA GCCTCTGTGT GCTGGGGCCC 
GGCGATGAAG CGCCTCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATCGAAAAC 
AATCTGCTCA GGCCAACTGG CGTGGCCCTT CGAGGAGCCC ACTTCTGCCT GAAGGTCTTC 
AGAGCTGAGG ACTTACCACA GATGGATGAT GCCGTGATGG ACAACGTGAA GCAGATCTTC 
GGCTTTGACA GTAACAAGAA GAACTTGGTG GATCCCTTCA TGGAAGTCAG CTTTGCGGGG 
AAAATGCTCT GCAGCAAGAT CCTGGAGAAA ACAGCCAACC CTCAGTGGAA CCAGAGCATC 
ACGCTGCCCG CCATGTTTCC CTCCATGTGT GAAAAAATGA GGATTCGCGT CATGGACTGG 
GACCGCCTCA CCCACAATGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCC 
GCCCCTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTGG CCAAGCCTTC TCCAGCCATG 
GACCTGGACG ACCATCTGGG CTTCCTCCCC ACCTTCGGGC CCTGCTACGT CAACCTCTAC 
GGCAGCCCCA GGGAGTTCAC CGGCTTCCCG GACCCCTACG CAGAACTCAA CATGGGCAAG 
GGAGAAGGTG TGGCCTACCG AGGTCGGCTT CTGCTCTCCC TGGAGACCAA GCTGGTGGAG 
CACAGCGACC AAAAGGTGGA GGACCTCCCT GCGGATGACA TCCTTCGGGT GGAGAAGTAT 
CTCCGGAGGC GTAAGTACTC CCTCTTTGCC GCCTTCTACT CGGCCTCCAT GCTCCAGGAT 
GTGGATGATG CCATCCAGTT CGAGGTCAGC ATCGGGAACT ATGGCAACAA GTTCGACACC 
ACGTGCCTGC CGCTGGCCTC TACCACCCAG TACAGCCGGG CGGTCTTTGA CGGGTGCCAC 
TACTACTACT TGCCCTGGGG CAACGTGAAG CCCGTGGTGG TGCTGTCATC CTACTGGGAG 
GACATCAGCC ACAGAGTTGA GGCTCAGAAC CAGCTGCTCA GGATTGCGGA CCGGCTGGAA 
GCTGCCCTGG AGCAAGTCCA CTTGGCCGTG AAGGCGCAGT ACAACGCCGA GGACGTGGAC 
TCCCTGTTGG CTCAGCTGAT GGATGAGCTC ATCTCAGACT GCAGCCAGCC CCTAGGCGAT 
GTCCACGAGA CACCCTCTGC CACCCACCTG GACCAGTACC TGCACAGGCT GCGTAGCCGT 
CACCTAAGCC AGATTGCAGA GGCGGCGCTG GCCCTGAAGC TCGGGCACGG CGGGCTCCCG 
GCCGCCCTGG AGCAGGCTGA AGACTGGCTC CTGCGGCTCC GTGCCCTGGC CGAGGAGCCT 
CAGAACAGCC TGCCCGACAT CATCATCTGG ATGCTGCAGG GCGACAAGCG AGTGGCATAC 
CAGCGGGTGC CTACACATGA GGTCCTCTTC TCCCGGCGTG GAGCCAGCTA CTGCGGCAAG 
AACTGTGGGA AGCTGCAGAC GATATTTCTC AAATATCCCA TGCAGGGGGT GCCCGGAGCC 
CGGATGCCCG TGCAGATACG GGTGAAGCTC TGGTTTGGGC TTTCTGTGGA TGAGAAGGAG 
TTCAACCAGT TTGCTGAGGG AAAGCTGTCT GTCTTTGCTG AAACCTATGA GAACCAGACC 
AAACTGGCCC TCGTTGGGAA CTGGGGCACA ACAGGCCTTA CCTACCCCAA GTTTTCTGAT 
GTCGCGGGCA AGATCAAGCT CCCCAAGGAT AGCTTCCGCC CCTCAGCCGG CTGGACCTGG 
GCTGGAGATT GGTTTGTGTG TCCAGAGAAG ACGCTGCTCC ATGACACCGA CGCGGGCCAC 
CTGAGTTTCG TGGCGGAGGT GTTTGAGAAC CAGACCCGGC TCCCTGGAGG CCACTGGGTC 
TACATGAGTG ACAACTACAC GGATGTGAAT GGGGAGAAGG TGCTTCCCAA GGATGACATT 
GAGTGCCCGC TGGGCTGGAA GTGGGAAGAT GAAGAGTGGT CCACGGACCT CAATCGGGCC 
GTTGATGAGC AAGGCTGGGA GTACAGTATC ACCATCCCCC CGGACCGGAA GCCGAAGCAC 
TGGGTCCCCG CTGAGAAGAT GTACTACACG CACCGTCGGC GGCGCTGGGT CCGCCTGCGC 
AGGAGGGACC TCAGCCAGAT GGAGGCCCTG AGGAAGCACA GGCAGGCGGA GGCGGAGGGC 
GAGGGCTGGG AGTACGCCTC GCTCTTTGGC TGGAAATTCC ACCTGGAGTA CCGCAAAACG 
GATGCATTCC GTCGCCGCCG CTGGCGCCGC CGCATGGAGC CGCTGGAGAA GACGGGGCCT 
GCAGCCGTGT TTGCCCTTGA GGGGGCACTG GGTGGCGTGG TAGATGACCG GAGTGAAGAT 
TCCATGTCCG TGTCCACCTT GAGCTTTGGC GTGAACAGAC CCACGATTTC CTGCATCTTT 
GATCATGGGA ACCGCTACCA CCTGCGCTGC TATATGTACC AGGCTCGGGA CCTGCCCTCG 
ATGGACAAGG ACTCTTTTTC TGATCCCTAC GCCATCGTCT CCTTCCTGCA CCAGAGCCAG 
AAGACCGTGG TGGTGAAGAA CACTCTGAAC CCCACCTGGG ACCAGACGCT CATCTTCTAT 
GAGATTGAGA TCTTTGGAGA GCCAGCCAGC ATCGCCGAGC AGCCGCCCAG CATTGTGGTG 
GAGCTGTATG ACCACGACAC CTACGGTGCA GATGAGTTTT TGGGACGCTG CATGTGTCAG 
CCCAGTCTGG AACGGATGCC CCGGCTGGCC TGGTTCCCGC TGGTTAGGGG CAGCCAGCCG 
GCAGGCGAGC TGCTGGCCTC TTTTGAGCTC ATCCAGAGAG AGAAGCCGGC CATTCACCAT 
ATTCCTGGTT TTGAGGTGCA GGATACATCG GGAATCCTGG ACGAGTCGGA GGACACAGAC 
CTGCCCTACC CACCGCCTCA GAGGGAGGCC AACATCTACA TGGTTCCCCA GAACATCAAG 
CCGGTTCTCC AGCGCACTGC CATAGAGATC CTGGCATGGG GCCTACGGAA CATGAAGAGT 
TACCAGCTGG CCAGTATCTC CTCCCCCAGC CTCGTGGTGG AGTGCGGGGG CCAGACGGTG 
CAGTCCTGTG TCATCAGGAA CCTCCGGAAG AACCCCAACT TCGACATCTG CACCCTCTTC 
ATGGAAGTGA TGCTGCCCAG AGAGGAGCTC TACTGTCCCC CCATCGTGAT CAAGGTCATT 
GATAACCGCC AGTTTGGCCG CCGGCCTGTG GTGGGCCAGT GTACCATACG CTCCCTGGAG 
AGCTTCATGT GTGACCTGTA CTCCGAGGAG AGTCCGTCCC CACAGGGCGG CCCAGATGAT 
GTGAGTTTAC TCAGTCCTGG GGAAGACGTG CTCATTGACA TCGACGACAA GGAGCCCCTC 
ATCCCCATAC AGGAGGAGGA GTTCATCGAC TGGTGGAGCA AATTCTTTGC CTCCACAGGG 
GAGAGGGAGA AGTGCGGCTC GTACTTGGAG AAGGGCTTTG ACACTCTAAA GGTCTATGAC 
ACACAACTGG AGAACGTGGA GGCCTTTGGG GGCCTGTCTG ACTTTTGTAA CACCTTCAAG 
CTCTACCGGG GCAAGACTCA GGAGGAGACG GAGGACCCAT CTGTTATCGG GGAGTTTAAG 
GGCCTCTTCA AAATCTACCC CCTCCCGGAA GACCCGGCCG TCCCCATGCC CCCGAGACAG 
TTCCAGCAGC TGGCTGCCCA GGGGCCTCAG GACTGCCTGG TCCGCATCTA TATCATCCGG 
GCATTTGGCC TGCAGCCCAA GGACCCCAAT GGGAAGTGTG ATCCTTACAT CAAGATCTCC 
ATAGGGAAGA AATCAGTGAG TGACCAGGAT AATTACATTC CCTGTACCCT GGAGCCTGTG 
TTTGGAAAGA TGTTCGAGCT GACCTGCACT CTGCCTCTGG AGAAGGACCT GAAGATCACA 
CTCTACGACT ATGACCTCCT CTCCAAAGAT GAGAAGATCG GGGAGACAGT CATCGACCTG 
GAGAATAGGC TGCTGTCCAA GTTCGGGGCT CGCTGCGGAC TCCCACAGAC CTACTGTGTC 
TCTGGACCCA ACCAGTGGAG GGACCAGCTC CGCCCCTCCC AGCTTCTCCA GCTCACCTGC 
CAGCAGCGCA GACTCAAGGC GCCCGTGTAC CTCACAGACC GCGTACTCTT TCAGGATAAA 
GAATACACCA TTGAGGAAAT AGAGGCTGGC AGGGTCCTGA ACCCACACCT GGGCCCAGTG 
GAAGAGCGTC TGGCCTTGCA CGTCCTCCAG CAGCTGGGCC TGGTCCCCGA GCATGTGGAG 
TCCCGGCCTC TTTACAGCCC CCTGCAGCCA GACATTGAGC AGGGGAAGCT GCAGATGTGG 
ATCGATCTGT TTCCCAAGGC CCTGGGGCGA CCTGGACCTC CCTTCAACAT CACCCCACGG 
AGAGCAAGAA GGTTTTTCCT GCGTTGTATT ATCTGGAACA CAAAGGACGT GATCCTGGAC 
GACCTCAGCA TCACGGGGGA GAAGATGAGT GACATTTACG TGAAAGGTTG GATGGTCGGC 
TTTGAAGAGC ACAAGCAGAA GACGGACGTG CACTACCGCT CCCTGGGAGG CGAAGGCAAC 
TTTAACTGGA GGTTCATTTT CCCCTTTGAC TACCTGCCCG CTGAGCAAGT CTGCACCGTC 
TCTAAGAAGG ATGCTTTCTG GAGGCTGGAC AAGACTGAGA GCAAAATCCC AGCACAAGTG 
GTATTTCAGA TCTGGGACAA TGACAAGTTC TCCTTTGACG ACTTTCTGGG CTCGTTGCAG 
TTGGATCTCA ACCGCATGCC CAAGCCTGCC AAGACAGCTG AGAAGTGCTC CTTGGACCAG 
CTGGACGACA CTTTCCACCC GGAGTGGTTT GTGTCCCTTT TTGAACAGAA AACAGTAAAG 
GGCTGGTGGC CCTGTGTGGC AGAAGACGGT GAGAAGAAGA TCCTGGCGGG CAAGCTGGAA 
ATGACCTTGG AGATCGTAGC AGAGAGTGAG CACGAAGAGC GGCCTGCGGG TCAAGGCCGG 
GATGAACCCA ACATGAACCC CAAGCTCGAG GACCCAAGGC GCCCCGACAC CTCCTTCCTG 
TGGTTCACCT CTCCGTACAA GACCATGAAG TTCATCCTGT GGCGGCGGTT CCGGTGCGCC 
ATCATCCTCT TCGTCGTTCT CGTCATCCTG CTGCTCTTCC TGGGCATCTT CCTCTATTCC 
TTCCCGAACT ATGCTGCCAT GAAGCTGGTG AAACCCTTCA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019650386.1
CDS	150..6494
Translation	MLSCLLVRASNLPSVKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEAQVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPSASLEPSPTLPDMDMVAGGGQSRAETWSLLSDSTVDTRYSGKKWLAPTDTGGEEDTEDQGLTGDEAEPLLDPSGAPGPGAPTTPRKPPSHPPPYHPGSKRKRGAPTPRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVMDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGVAKPSPAMDLDDHLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRLLLSLETKLVEHSDQKVEDLPADDILRVEKYLRRRKYSLFAAFYSASMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADRLEAALEQVHLAVKAQYNAEDVDSLLAQLMDELISDCSQPLGDVHETPSATHLDQYLHRLRSRHLSQIAEAALALKLGHGGLPAALEQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPTHEVLFSRRGASYCGKNCGKLQTIFLKYPMQGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVAGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDTDAGHLSFVAEVFENQTRLPGGHWVYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALRKHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSMSVSTLSFGVNRPTISCIFDHGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFLGRCMCQPSLERMPRLAWFPLVRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTSGILDESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPIVIKVIDNRQFGRRPVVGQCTIRSLESFMCDLYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASTGEREKCGSYLEKGFDTLKVYDTQLENVEAFGGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAVPMPPRQFQQLAAQGPQDCLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLQLTCQQRRLKAPVYLTDRVLFQDKEYTIEEIEAGRVLNPHLGPVEERLALHVLQQLGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPAQVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEDGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFVVLVILLLFLGIFLYSFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_019794827.1
Organism	Ailuropoda melanoleuca(giant panda)
Definition	Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019794827.1

ORF Insert Sequence:

ATGCTGAGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGTGAAGAA GGACCGGCGC 
AGTGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCAGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTCCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCGG GAGGTCCTCG CCACCCCCAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCCTC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGTGGCTG GTGGGGGACA GAGCCGGGCT 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACGGGAGAT 
GAGGCAGAGC CATTACTGGA TCCGAGCGGT GCCCCAGGCC CTGGGGCCCC CACCACCCCG 
AGGAAGCCAC CTTCCCATCC GCCCCCCTAC CACCCCGGGA GCAAGAGAAA AAGAGGTGCT 
CCCACGCCCA GAAAGCTGCT GTCGGACAAA CCGCAGGACT TCCAGATCAG GGTGCAAGTG 
ATCGAGGGGC GCCAGCTGCC AGGGGTGAAT ATCAAGCCTG TGGTCAAAGT CACGGCTGCC 
GGGCAGACCA AGAGGACACG GATCCACAAG GGAAACAGCC CACTCTTCAA CGAGACTCTC 
TTCTTCAATG TGTTTGACTC TCCCTCGGAG CTGTTTGACG AGCCCATCTT TATCACGGTG 
GTAGACTCCC GTTCTCTCCG GACAGACGCT CTCATTGGGG AATTCCGGAT GGACGTGGGT 
ACCATCTACA GAGAGCCCCG ACACGCCTAT CTGAGGAAAT GGCTTCTCCT CTCGGACCCT 
GACGATTTCT CTGCTGGGGC CAGAGGTTAC CTGAAAGCAA GCCTCTGTGT GCTGGGGCCC 
GGCGATGAAG CGCCTCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATCGAAAAC 
AATCTGCTCA GGCCAACTGG CGTGGCCCTT CGAGGAGCCC ACTTCTGCCT GAAGGTCTTC 
AGAGCTGAGG ACTTACCACA GATGGATGAT GCCGTGATGG ACAACGTGAA GCAGATCTTC 
GGCTTTGACA GTAACAAGAA GAACTTGGTG GATCCCTTCA TGGAAGTCAG CTTTGCGGGG 
AAAATGCTCT GCAGCAAGAT CCTGGAGAAA ACAGCCAACC CTCAGTGGAA CCAGAGCATC 
ACGCTGCCCG CCATGTTTCC CTCCATGTGT GAAAAAATGA GGATTCGCGT CATGGACTGG 
GACCGCCTCA CCCACAATGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCC 
GCCCCTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTGG CCAAGCCTTC TCCAGCCATG 
GACCTGGACG ACCATCTGGG CTTCCTCCCC ACCTTCGGGC CCTGCTACGT CAACCTCTAC 
GGCAGCCCCA GGGAGTTCAC CGGCTTCCCG GACCCCTACG CAGAACTCAA CATGGGCAAG 
GGAGAAGGTG TGGCCTACCG AGGTCGGCTT CTGCTCTCCC TGGAGACCAA GCTGGTGGAG 
CACAGCGACC AAAAGGTGGA GGACCTCCCT GCGGATGACA TCCTTCGGGT GGAGAAGTAT 
CTCCGGAGGC GTAAGTACTC CCTCTTTGCC GCCTTCTACT CGGCCTCCAT GCTCCAGGAT 
GTGGATGATG CCATCCAGTT CGAGGTCAGC ATCGGGAACT ATGGCAACAA GTTCGACACC 
ACGTGCCTGC CGCTGGCCTC TACCACCCAG TACAGCCGGG CGGTCTTTGA CGGGTGCCAC 
TACTACTACT TGCCCTGGGG CAACGTGAAG CCCGTGGTGG TGCTGTCATC CTACTGGGAG 
GACATCAGCC ACAGAGTTGA GGCTCAGAAC CAGCTGCTCA GGATTGCGGA CCGGCTGGAA 
GCTGCCCTGG AGCAAGTCCA CTTGGCCGTG AAGGCGCAGT ACAACGCCGA GGACGTGGAC 
TCCCTGTTGG CTCAGCTGAT GGATGAGCTC ATCTCAGACT GCAGCCAGCC CCTAGGCGAT 
GTCCACGAGA CACCCTCTGC CACCCACCTG GACCAGTACC TGCACAGGCT GCGTAGCCGT 
CACCTAAGCC AGATTGCAGA GGCGGCGCTG GCCCTGAAGC TCGGGCACGG CGGGCTCCCG 
GCCGCCCTGG AGCAGGCTGA AGACTGGCTC CTGCGGCTCC GTGCCCTGGC CGAGGAGCCT 
CAGAACAGCC TGCCCGACAT CATCATCTGG ATGCTGCAGG GCGACAAGCG AGTGGCATAC 
CAGCGGGTGC CTACACATGA GGTCCTCTTC TCCCGGCGTG GAGCCAGCTA CTGCGGCAAG 
AACTGTGGGA AGCTGCAGAC GATATTTCTC AAATATCCCA TGCAGGGGGT GCCCGGAGCC 
CGGATGCCCG TGCAGATACG GGTGAAGCTC TGGTTTGGGC TTTCTGTGGA TGAGAAGGAG 
TTCAACCAGT TTGCTGAGGG AAAGCTGTCT GTCTTTGCTG AAACCTATGA GAACCAGACC 
AAACTGGCCC TCGTTGGGAA CTGGGGCACA ACAGGCCTTA CCTACCCCAA GTTTTCTGAT 
GTCGCGGGCA AGATCAAGCT CCCCAAGGAT AGCTTCCGCC CCTCAGCCGG CTGGACCTGG 
GCTGGAGATT GGTTTGTGTG TCCAGAGAAG ACGCTGCTCC ATGACACCGA CGCGGGCCAC 
CTGAGTTTCG TGGCGGAGGT GTTTGAGAAC CAGACCCGGC TCCCTGGAGG CCACTGGGTC 
TACATGAGTG ACAACTACAC GGATGTGAAT GGGGAGAAGG TGCTTCCCAA GGATGACATT 
GAGTGCCCGC TGGGCTGGAA GTGGGAAGAT GAAGAGTGGT CCACGGACCT CAATCGGGCC 
GTTGATGAGC AAGGCTGGGA GTACAGTATC ACCATCCCCC CGGACCGGAA GCCGAAGCAC 
TGGGTCCCCG CTGAGAAGAT GTACTACACG CACCGTCGGC GGCGCTGGGT CCGCCTGCGC 
AGGAGGGACC TCAGCCAGAT GGAGGCCCTG AGGAAGCACA GGCAGGCGGA GGCGGAGGGC 
GAGGGCTGGG AGTACGCCTC GCTCTTTGGC TGGAAATTCC ACCTGGAGTA CCGCAAAACG 
GATGCATTCC GTCGCCGCCG CTGGCGCCGC CGCATGGAGC CGCTGGAGAA GACGGGGCCT 
GCAGCCGTGT TTGCCCTTGA GGGGGCACTG GGTGGCGTGG TAGATGACCG GAGTGAAGAT 
TCCATGTCCG TGTCCACCTT GAGCTTTGGC GTGAACAGAC CCACGATTTC CTGCATCTTT 
GATCATGGGA ACCGCTACCA CCTGCGCTGC TATATGTACC AGGCTCGGGA CCTGCCCTCG 
ATGGACAAGG ACTCTTTTTC TGATCCCTAC GCCATCGTCT CCTTCCTGCA CCAGAGCCAG 
AAGACCGTGG TGGTGAAGAA CACTCTGAAC CCCACCTGGG ACCAGACGCT CATCTTCTAT 
GAGATTGAGA TCTTTGGAGA GCCAGCCAGC ATCGCCGAGC AGCCGCCCAG CATTGTGGTG 
GAGCTGTATG ACCACGACAC CTACGGTGCA GATGAGTTTT TGGGACGCTG CATGTGTCAG 
CCCAGTCTGG AACGGATGCC CCGGCTGGCC TGGTTCCCGC TGGTTAGGGG CAGCCAGCCG 
GCAGGCGAGC TGCTGGCCTC TTTTGAGCTC ATCCAGAGAG AGAAGCCGGC CATTCACCAT 
ATTCCTGGTT TTGAGGTGCA GGATACATCG GGAATCCTGG ACGAGTCGGA GGACACAGAC 
CTGCCCTACC CACCGCCTCA GAGGGAGGCC AACATCTACA TGGTTCCCCA GAACATCAAG 
CCGGTTCTCC AGCGCACTGC CATAGAGATC CTGGCATGGG GCCTACGGAA CATGAAGAGT 
TACCAGCTGG CCAGTATCTC CTCCCCCAGC CTCGTGGTGG AGTGCGGGGG CCAGACGGTG 
CAGTCCTGTG TCATCAGGAA CCTCCGGAAG AACCCCAACT TCGACATCTG CACCCTCTTC 
ATGGAAGTGA TGCTGCCCAG AGAGGAGCTC TACTGTCCCC CCATCGTGAT CAAGGTCATT 
GATAACCGCC AGTTTGGCCG CCGGCCTGTG GTGGGCCAGT GTACCATACG CTCCCTGGAG 
AGCTTCATGT GTGACCTGTA CTCCGAGGAG AGTCCGTCCC CACAGGGCGG CCCAGATGAT 
GTGAGTTTAC TCAGTCCTGG GGAAGACGTG CTCATTGACA TCGACGACAA GGAGCCCCTC 
ATCCCCATAC AGGAGGAGGA GTTCATCGAC TGGTGGAGCA AATTCTTTGC CTCCACAGGG 
GAGAGGGAGA AGTGCGGCTC GTACTTGGAG AAGGGCTTTG ACACTCTAAA GGTCTATGAC 
ACACAACTGG AGAACGTGGA GGCCTTTGGG GGCCTGTCTG ACTTTTGTAA CACCTTCAAG 
CTCTACCGGG GCAAGACTCA GGAGGAGACG GAGGACCCAT CTGTTATCGG GGAGTTTAAG 
GGCCTCTTCA AAATCTACCC CCTCCCGGAA GACCCGGCCG TCCCCATGCC CCCGAGACAG 
TTCCAGCAGC TGGCTGCCCA GGGGCCTCAG GACTGCCTGG TCCGCATCTA TATCATCCGG 
GCATTTGGCC TGCAGCCCAA GGACCCCAAT GGGAAGTGTG ATCCTTACAT CAAGATCTCC 
ATAGGGAAGA AATCAGTGAG TGACCAGGAT AATTACATTC CCTGTACCCT GGAGCCTGTG 
TTTGGAAAGA TGTTCGAGCT GACCTGCACT CTGCCTCTGG AGAAGGACCT GAAGATCACA 
CTCTACGACT ATGACCTCCT CTCCAAAGAT GAGAAGATCG GGGAGACAGT CATCGACCTG 
GAGAATAGGC TGCTGTCCAA GTTCGGGGCT CGCTGCGGAC TCCCACAGAC CTACTGTGTC 
TCTGGACCCA ACCAGTGGAG GGACCAGCTC CGCCCCTCCC AGCTTCTCCA GCTCACCTGC 
CAGCAGCGCA GACTCAAGGC GCCCGTGTAC CTCACAGACC GCGTACTCTT TCAGGATAAA 
GAATACACCA TTGAGGAAAT AGAGGCTGGC AGGGTCCTGA ACCCACACCT GGGCCCAGTG 
GAAGAGCGTC TGGCCTTGCA CGTCCTCCAG CAGCTGGGCC TGGTCCCCGA GCATGTGGAG 
TCCCGGCCTC TTTACAGCCC CCTGCAGCCA GACATTGAGC AGGGGAAGCT GCAGATGTGG 
ATCGATCTGT TTCCCAAGGC CCTGGGGCGA CCTGGACCTC CCTTCAACAT CACCCCACGG 
AGAGCAAGAA GGTTTTTCCT GCGTTGTATT ATCTGGAACA CAAAGGACGT GATCCTGGAC 
GACCTCAGCA TCACGGGGGA GAAGATGAGT GACATTTACG TGAAAGGTTG GATGGTCGGC 
TTTGAAGAGC ACAAGCAGAA GACGGACGTG CACTACCGCT CCCTGGGAGG CGAAGGCAAC 
TTTAACTGGA GGTTCATTTT CCCCTTTGAC TACCTGCCCG CTGAGCAAGT CTGCACCGTC 
TCTAAGAAGG ATGCTTTCTG GAGGCTGGAC AAGACTGAGA GCAAAATCCC AGCACAAGTG 
GTATTTCAGA TCTGGGACAA TGACAAGTTC TCCTTTGACG ACTTTCTGGG CTCGTTGCAG 
TTGGATCTCA ACCGCATGCC CAAGCCTGCC AAGACAGCTG AGAAGTGCTC CTTGGACCAG 
CTGGACGACA CTTTCCACCC GGAGTGGTTT GTGTCCCTTT TTGAACAGAA AACAGTAAAG 
GGCTGGTGGC CCTGTGTGGC AGAAGACGGT GAGAAGAAGA TCCTGGCGGG CAAGCTGGAA 
ATGACCTTGG AGATCGTAGC AGAGAGTGAG CACGAAGAGC GGCCTGCGGG TCAAGGCCGG 
GATGAACCCA ACATGAACCC CAAGCTCGAG GACCCAAGGC GCCCCGACAC CTCCTTCCTG 
TGGTTCACCT CTCCGTACAA GACCATGAAG TTCATCCTGT GGCGGCGGTT CCGGTGCGCC 
ATCATCCTCT TCGTCGTTCT CGTCATCCTG CTGCTCTTCC TGGGCATCTT CCTCTATTCC 
TTCCCGAACT ATGCTGCCAT GAAGCTGGTG AAACCCTTCA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_019794828.1 ) cDNA ORF clone, Ailuropoda melanoleuca(giant panda) -> XP_019650387.1 Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X11, mRNA.

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CloneID	OAc89682
Clone ID Related Accession (Same CDS sequence)	XM_019794828.1
Accession Version	XM_019794828.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-27
Organism	Ailuropoda melanoleuca(giant panda)
Product	dysferlin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003217356.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ailuropoda melanoleuca Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTATGCCGAG AATGTCCACA CCCCGGACAC CGACATCAGC 
GATGCCTACT GCTCTGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CAGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGAGCTCCG AGCTCCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
TTAGGGGAAG CCCAAGTCCC ACTCCGGGAG GTCCTCGCCA CCCCCAGTCT GTCTGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CGCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCCTCTGC ATCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATATG GTGGCTGGTG GGGGACAGAG CCGGGCTGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCTG 
GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAGGACC AGGGGCTCAC GGGAGATGAG 
GCAGAGCCAT TACTGGATCC GAGCGGTGCC CCAGGCCCTG GGGCCCCCAC CACCCCGAGG 
AAGCCACCTT CCCATCCGCC CCCCTACCAC CCCGGGAGCA AGAGAAAAAG AGGTGCTCCC 
ACGCCCAGAA AGCTGCTGTC GGACAAACCG CAGGACTTCC AGATCAGGGT GCAAGTGATC 
GAGGGGCGCC AGCTGCCAGG GGTGAATATC AAGCCTGTGG TCAAAGTCAC GGCTGCCGGG 
CAGACCAAGA GGACACGGAT CCACAAGGGA AACAGCCCAC TCTTCAACGA GACTCTCTTC 
TTCAATGTGT TTGACTCTCC CTCGGAGCTG TTTGACGAGC CCATCTTTAT CACGGTGGTA 
GACTCCCGTT CTCTCCGGAC AGACGCTCTC ATTGGGGAAT TCCGGATGGA CGTGGGTACC 
ATCTACAGAG AGCCCCGACA CGCCTATCTG AGGAAATGGC TTCTCCTCTC GGACCCTGAC 
GATTTCTCTG CTGGGGCCAG AGGTTACCTG AAAGCAAGCC TCTGTGTGCT GGGGCCCGGC 
GATGAAGCGC CTCTGGAGAG AAAGGACCCC TCTGAAGACA AGGAGGACAT CGAAAACAAT 
CTGCTCAGGC CAACTGGCGT GGCCCTTCGA GGAGCCCACT TCTGCCTGAA GGTCTTCAGA 
GCTGAGGACT TACCACAGAT GGATGATGCC GTGATGGACA ACGTGAAGCA GATCTTCGGC 
TTTGACAGTA ACAAGAAGAA CTTGGTGGAT CCCTTCATGG AAGTCAGCTT TGCGGGGAAA 
ATGCTCTGCA GCAAGATCCT GGAGAAAACA GCCAACCCTC AGTGGAACCA GAGCATCACG 
CTGCCCGCCA TGTTTCCCTC CATGTGTGAA AAAATGAGGA TTCGCGTCAT GGACTGGGAC 
CGCCTCACCC ACAATGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCCGCC 
CCTGGAGGAG AAATAGAAGA GGAGCCTGCA GGTGTGGCCA AGCCTTCTCC AGCCATGGAC 
CTGGACGACC ATCTGGGCTT CCTCCCCACC TTCGGGCCCT GCTACGTCAA CCTCTACGGC 
AGCCCCAGGG AGTTCACCGG CTTCCCGGAC CCCTACGCAG AACTCAACAT GGGCAAGGGA 
GAAGGTGTGG CCTACCGAGG TCGGCTTCTG CTCTCCCTGG AGACCAAGCT GGTGGAGCAC 
AGCGACCAAA AGGTGGAGGA CCTCCCTGCG GATGACATCC TTCGGGTGGA GAAGTATCTC 
CGGAGGCGTA AGTACTCCCT CTTTGCCGCC TTCTACTCGG CCTCCATGCT CCAGGATGTG 
GATGATGCCA TCCAGTTCGA GGTCAGCATC GGGAACTATG GCAACAAGTT CGACACCACG 
TGCCTGCCGC TGGCCTCTAC CACCCAGTAC AGCCGGGCGG TCTTTGACGG GTGCCACTAC 
TACTACTTGC CCTGGGGCAA CGTGAAGCCC GTGGTGGTGC TGTCATCCTA CTGGGAGGAC 
ATCAGCCACA GAGTTGAGGC TCAGAACCAG CTGCTCAGGA TTGCGGACCG GCTGGAAGCT 
GCCCTGGAGC AAGTCCACTT GGCCGTGAAG GCGCAGTACA ACGCCGAGGA CGTGGACTCC 
CTGTTGGCTC AGCTGATGGA TGAGCTCATC TCAGACTGCA GCCAGCCCCT AGGCGATGTC 
CACGAGACAC CCTCTGCCAC CCACCTGGAC CAGTACCTGC ACAGGCTGCG TAGCCGTCAC 
CTAAGCCAGA TTGCAGAGGC GGCGCTGGCC CTGAAGCTCG GGCACGGCGG GCTCCCGGCC 
GCCCTGGAGC AGGCTGAAGA CTGGCTCCTG CGGCTCCGTG CCCTGGCCGA GGAGCCTCAG 
AACAGCCTGC CCGACATCAT CATCTGGATG CTGCAGGGCG ACAAGCGAGT GGCATACCAG 
CGGGTGCCTA CACATGAGGT CCTCTTCTCC CGGCGTGGAG CCAGCTACTG CGGCAAGAAC 
TGTGGGAAGC TGCAGACGAT ATTTCTCAAA TATCCCATGC AGGGGGTGCC CGGAGCCCGG 
ATGCCCGTGC AGATACGGGT GAAGCTCTGG TTTGGGCTTT CTGTGGATGA GAAGGAGTTC 
AACCAGTTTG CTGAGGGAAA GCTGTCTGTC TTTGCTGAAA CCTATGAGAA CCAGACCAAA 
CTGGCCCTCG TTGGGAACTG GGGCACAACA GGCCTTACCT ACCCCAAGTT TTCTGATGTC 
GCGGGCAAGA TCAAGCTCCC CAAGGATAGC TTCCGCCCCT CAGCCGGCTG GACCTGGGCT 
GGAGATTGGT TTGTGTGTCC AGAGAAGACG CTGCTCCATG ACACCGACGC GGGCCACCTG 
AGTTTCGTGG CGGAGGTGTT TGAGAACCAG ACCCGGCTCC CTGGAGGCCA CTGGGTCTAC 
ATGAGTGACA ACTACACGGA TGTGAATGGG GAGAAGGTGC TTCCCAAGGA TGACATTGAG 
TGCCCGCTGG GCTGGAAGTG GGAAGATGAA GAGTGGTCCA CGGACCTCAA TCGGGCCGTT 
GATGAGCAAG GCTGGGAGTA CAGTATCACC ATCCCCCCGG ACCGGAAGCC GAAGCACTGG 
GTCCCCGCTG AGAAGATGTA CTACACGCAC CGTCGGCGGC GCTGGGTCCG CCTGCGCAGG 
AGGGACCTCA GCCAGATGGA GGCCCTGAGG AAGCACAGGC AGGCGGAGGC GGAGGGCGAG 
GGCTGGGAGT ACGCCTCGCT CTTTGGCTGG AAATTCCACC TGGAGTACCG CAAAACGGAT 
GCATTCCGTC GCCGCCGCTG GCGCCGCCGC ATGGAGCCGC TGGAGAAGAC GGGGCCTGCA 
GCCGTGTTTG CCCTTGAGGG GGCACTGGGT GGCGTGGTAG ATGACCGGAG TGAAGATTCC 
ATGTCCGTGT CCACCTTGAG CTTTGGCGTG AACAGACCCA CGATTTCCTG CATCTTTGAT 
CATGGGAACC GCTACCACCT GCGCTGCTAT ATGTACCAGG CTCGGGACCT GCCCTCGATG 
GACAAGGACT CTTTTTCTGA TCCCTACGCC ATCGTCTCCT TCCTGCACCA GAGCCAGAAG 
ACCGTGGTGG TGAAGAACAC TCTGAACCCC ACCTGGGACC AGACGCTCAT CTTCTATGAG 
ATTGAGATCT TTGGAGAGCC AGCCAGCATC GCCGAGCAGC CGCCCAGCAT TGTGGTGGAG 
CTGTATGACC ACGACACCTA CGGTGCAGAT GAGTTTTTGG GACGCTGCAT GTGTCAGCCC 
AGTCTGGAAC GGATGCCCCG GCTGGCCTGG TTCCCGCTGG TTAGGGGCAG CCAGCCGGCA 
GGCGAGCTGC TGGCCTCTTT TGAGCTCATC CAGAGAGAGA AGCCGGCCAT TCACCATATT 
CCTGGTTTTG AGGTGCAGGA TACATCGGGA ATCCTGGACG AGTCGGAGGA CACAGACCTG 
CCCTACCCAC CGCCTCAGAG GGAGGCCAAC ATCTACATGG TTCCCCAGAA CATCAAGCCG 
GTTCTCCAGC GCACTGCCAT AGAGATCCTG GCATGGGGCC TACGGAACAT GAAGAGTTAC 
CAGCTGGCCA GTATCTCCTC CCCCAGCCTC GTGGTGGAGT GCGGGGGCCA GACGGTGCAG 
TCCTGTGTCA TCAGGAACCT CCGGAAGAAC CCCAACTTCG ACATCTGCAC CCTCTTCATG 
GAAGTGATGC TGCCCAGAGA GGAGCTCTAC TGTCCCCCCA TCGTGATCAA GGTCATTGAT 
AACCGCCAGT TTGGCCGCCG GCCTGTGGTG GGCCAGTGTA CCATACGCTC CCTGGAGAGC 
TTCATGTGTG ACCTGTACTC CGAGGAGAGT CCGTCCCCAC AGGGCGGCCC AGATGATGTG 
AGTTTACTCA GTCCTGGGGA AGACGTGCTC ATTGACATCG ACGACAAGGA GCCCCTCATC 
CCCATACAGG AGGAGGAGTT CATCGACTGG TGGAGCAAAT TCTTTGCCTC CACAGGGGAG 
AGGGAGAAGT GCGGCTCGTA CTTGGAGAAG GGCTTTGACA CTCTAAAGGT CTATGACACA 
CAACTGGAGA ACGTGGAGGC CTTTGGGGGC CTGTCTGACT TTTGTAACAC CTTCAAGCTC 
TACCGGGGCA AGACTCAGGA GGAGACGGAG GACCCATCTG TTATCGGGGA GTTTAAGGGC 
CTCTTCAAAA TCTACCCCCT CCCGGAAGAC CCGGCCGTCC CCATGCCCCC GAGACAGTTC 
CAGCAGCTGG CTGCCCAGGG GCCTCAGGAC TGCCTGGTCC GCATCTATAT CATCCGGGCA 
TTTGGCCTGC AGCCCAAGGA CCCCAATGGG AAGTGTGATC CTTACATCAA GATCTCCATA 
GGGAAGAAAT CAGTGAGTGA CCAGGATAAT TACATTCCCT GTACCCTGGA GCCTGTGTTT 
GGAAAGATGT TCGAGCTGAC CTGCACTCTG CCTCTGGAGA AGGACCTGAA GATCACACTC 
TACGACTATG ACCTCCTCTC CAAAGATGAG AAGATCGGGG AGACAGTCAT CGACCTGGAG 
AATAGGCTGC TGTCCAAGTT CGGGGCTCGC TGCGGACTCC CACAGACCTA CTGTGTCTCT 
GGACCCAACC AGTGGAGGGA CCAGCTCCGC CCCTCCCAGC TTCTCCAGCT CACCTGCCAG 
CAGCGCAGAC TCAAGGCGCC CGTGTACCTC ACAGACCGCG TACTCTTTCA GGATAAAGAA 
TACACCATTG AGGAAATAGA GGCTGGCAGG GTCCTGAACC CACACCTGGG CCCAGTGGAA 
GAGCGTCTGG CCTTGCACGT CCTCCAGCAG CTGGGCCTGG TCCCCGAGCA TGTGGAGTCC 
CGGCCTCTTT ACAGCCCCCT GCAGCCAGAC ATTGAGCAGG GGAAGCTGCA GATGTGGATC 
GATCTGTTTC CCAAGGCCCT GGGGCGACCT GGACCTCCCT TCAACATCAC CCCACGGAGA 
GCAAGAAGGT TTTTCCTGCG TTGTATTATC TGGAACACAA AGGACGTGAT CCTGGACGAC 
CTCAGCATCA CGGGGGAGAA GATGAGTGAC ATTTACGTGA AAGGTTGGAT GGTCGGCTTT 
GAAGAGCACA AGCAGAAGAC GGACGTGCAC TACCGCTCCC TGGGAGGCGA AGGCAACTTT 
AACTGGAGGT TCATTTTCCC CTTTGACTAC CTGCCCGCTG AGCAAGTCTG CACCGTCTCT 
AAGAAGGATG CTTTCTGGAG GCTGGACAAG ACTGAGAGCA AAATCCCAGC ACAAGTGGTA 
TTTCAGATCT GGGACAATGA CAAGTTCTCC TTTGACGACT TTCTGGGCTC GTTGCAGTTG 
GATCTCAACC GCATGCCCAA GCCTGCCAAG ACAGCTGAGA AGTGCTCCTT GGACCAGCTG 
GACGACACTT TCCACCCGGA GTGGTTTGTG TCCCTTTTTG AACAGAAAAC AGTAAAGGGC 
TGGTGGCCCT GTGTGGCAGA AGACGGTGAG AAGAAGATCC TGGCGGGCAA GCTGGAAATG 
ACCTTGGAGA TCGTAGCAGA GAGTGAGCAC GAAGAGCGGC CTGCGGGTCA AGGCCGGGAT 
GAACCCAACA TGAACCCCAA GCTCGAGGAC CCAAGGCGCC CCGACACCTC CTTCCTGTGG 
TTCACCTCTC CGTACAAGAC CATGAAGTTC ATCCTGTGGC GGCGGTTCCG GTGCGCCATC 
ATCCTCTTCG TCGTTCTCGT CATCCTGCTG CTCTTCCTGG GCATCTTCCT CTATTCCTTC 
CCGAACTATG CTGCCATGAA GCTGGTGAAA CCCTTCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019650387.1
CDS	487..6828
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEAQVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPSASLEPSPTLPDMDMVAGGGQSRAETWSLLSDSTVDTRYSGKKWLAPTDTGGEEDTEDQGLTGDEAEPLLDPSGAPGPGAPTTPRKPPSHPPPYHPGSKRKRGAPTPRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVMDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGVAKPSPAMDLDDHLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRLLLSLETKLVEHSDQKVEDLPADDILRVEKYLRRRKYSLFAAFYSASMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADRLEAALEQVHLAVKAQYNAEDVDSLLAQLMDELISDCSQPLGDVHETPSATHLDQYLHRLRSRHLSQIAEAALALKLGHGGLPAALEQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPTHEVLFSRRGASYCGKNCGKLQTIFLKYPMQGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVAGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDTDAGHLSFVAEVFENQTRLPGGHWVYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALRKHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSMSVSTLSFGVNRPTISCIFDHGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFLGRCMCQPSLERMPRLAWFPLVRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTSGILDESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPIVIKVIDNRQFGRRPVVGQCTIRSLESFMCDLYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASTGEREKCGSYLEKGFDTLKVYDTQLENVEAFGGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAVPMPPRQFQQLAAQGPQDCLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLQLTCQQRRLKAPVYLTDRVLFQDKEYTIEEIEAGRVLNPHLGPVEERLALHVLQQLGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPAQVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEDGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFVVLVILLLFLGIFLYSFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_019794828.1
Organism	Ailuropoda melanoleuca(giant panda)
Definition	Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019794828.1

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCCGAG AATGTCCACA CCCCGGACAC CGACATCAGC 
GATGCCTACT GCTCTGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CAGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGAGCTCCG AGCTCCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
TTAGGGGAAG CCCAAGTCCC ACTCCGGGAG GTCCTCGCCA CCCCCAGTCT GTCTGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CGCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCCTCTGC ATCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATATG GTGGCTGGTG GGGGACAGAG CCGGGCTGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCTG 
GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAGGACC AGGGGCTCAC GGGAGATGAG 
GCAGAGCCAT TACTGGATCC GAGCGGTGCC CCAGGCCCTG GGGCCCCCAC CACCCCGAGG 
AAGCCACCTT CCCATCCGCC CCCCTACCAC CCCGGGAGCA AGAGAAAAAG AGGTGCTCCC 
ACGCCCAGAA AGCTGCTGTC GGACAAACCG CAGGACTTCC AGATCAGGGT GCAAGTGATC 
GAGGGGCGCC AGCTGCCAGG GGTGAATATC AAGCCTGTGG TCAAAGTCAC GGCTGCCGGG 
CAGACCAAGA GGACACGGAT CCACAAGGGA AACAGCCCAC TCTTCAACGA GACTCTCTTC 
TTCAATGTGT TTGACTCTCC CTCGGAGCTG TTTGACGAGC CCATCTTTAT CACGGTGGTA 
GACTCCCGTT CTCTCCGGAC AGACGCTCTC ATTGGGGAAT TCCGGATGGA CGTGGGTACC 
ATCTACAGAG AGCCCCGACA CGCCTATCTG AGGAAATGGC TTCTCCTCTC GGACCCTGAC 
GATTTCTCTG CTGGGGCCAG AGGTTACCTG AAAGCAAGCC TCTGTGTGCT GGGGCCCGGC 
GATGAAGCGC CTCTGGAGAG AAAGGACCCC TCTGAAGACA AGGAGGACAT CGAAAACAAT 
CTGCTCAGGC CAACTGGCGT GGCCCTTCGA GGAGCCCACT TCTGCCTGAA GGTCTTCAGA 
GCTGAGGACT TACCACAGAT GGATGATGCC GTGATGGACA ACGTGAAGCA GATCTTCGGC 
TTTGACAGTA ACAAGAAGAA CTTGGTGGAT CCCTTCATGG AAGTCAGCTT TGCGGGGAAA 
ATGCTCTGCA GCAAGATCCT GGAGAAAACA GCCAACCCTC AGTGGAACCA GAGCATCACG 
CTGCCCGCCA TGTTTCCCTC CATGTGTGAA AAAATGAGGA TTCGCGTCAT GGACTGGGAC 
CGCCTCACCC ACAATGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCCGCC 
CCTGGAGGAG AAATAGAAGA GGAGCCTGCA GGTGTGGCCA AGCCTTCTCC AGCCATGGAC 
CTGGACGACC ATCTGGGCTT CCTCCCCACC TTCGGGCCCT GCTACGTCAA CCTCTACGGC 
AGCCCCAGGG AGTTCACCGG CTTCCCGGAC CCCTACGCAG AACTCAACAT GGGCAAGGGA 
GAAGGTGTGG CCTACCGAGG TCGGCTTCTG CTCTCCCTGG AGACCAAGCT GGTGGAGCAC 
AGCGACCAAA AGGTGGAGGA CCTCCCTGCG GATGACATCC TTCGGGTGGA GAAGTATCTC 
CGGAGGCGTA AGTACTCCCT CTTTGCCGCC TTCTACTCGG CCTCCATGCT CCAGGATGTG 
GATGATGCCA TCCAGTTCGA GGTCAGCATC GGGAACTATG GCAACAAGTT CGACACCACG 
TGCCTGCCGC TGGCCTCTAC CACCCAGTAC AGCCGGGCGG TCTTTGACGG GTGCCACTAC 
TACTACTTGC CCTGGGGCAA CGTGAAGCCC GTGGTGGTGC TGTCATCCTA CTGGGAGGAC 
ATCAGCCACA GAGTTGAGGC TCAGAACCAG CTGCTCAGGA TTGCGGACCG GCTGGAAGCT 
GCCCTGGAGC AAGTCCACTT GGCCGTGAAG GCGCAGTACA ACGCCGAGGA CGTGGACTCC 
CTGTTGGCTC AGCTGATGGA TGAGCTCATC TCAGACTGCA GCCAGCCCCT AGGCGATGTC 
CACGAGACAC CCTCTGCCAC CCACCTGGAC CAGTACCTGC ACAGGCTGCG TAGCCGTCAC 
CTAAGCCAGA TTGCAGAGGC GGCGCTGGCC CTGAAGCTCG GGCACGGCGG GCTCCCGGCC 
GCCCTGGAGC AGGCTGAAGA CTGGCTCCTG CGGCTCCGTG CCCTGGCCGA GGAGCCTCAG 
AACAGCCTGC CCGACATCAT CATCTGGATG CTGCAGGGCG ACAAGCGAGT GGCATACCAG 
CGGGTGCCTA CACATGAGGT CCTCTTCTCC CGGCGTGGAG CCAGCTACTG CGGCAAGAAC 
TGTGGGAAGC TGCAGACGAT ATTTCTCAAA TATCCCATGC AGGGGGTGCC CGGAGCCCGG 
ATGCCCGTGC AGATACGGGT GAAGCTCTGG TTTGGGCTTT CTGTGGATGA GAAGGAGTTC 
AACCAGTTTG CTGAGGGAAA GCTGTCTGTC TTTGCTGAAA CCTATGAGAA CCAGACCAAA 
CTGGCCCTCG TTGGGAACTG GGGCACAACA GGCCTTACCT ACCCCAAGTT TTCTGATGTC 
GCGGGCAAGA TCAAGCTCCC CAAGGATAGC TTCCGCCCCT CAGCCGGCTG GACCTGGGCT 
GGAGATTGGT TTGTGTGTCC AGAGAAGACG CTGCTCCATG ACACCGACGC GGGCCACCTG 
AGTTTCGTGG CGGAGGTGTT TGAGAACCAG ACCCGGCTCC CTGGAGGCCA CTGGGTCTAC 
ATGAGTGACA ACTACACGGA TGTGAATGGG GAGAAGGTGC TTCCCAAGGA TGACATTGAG 
TGCCCGCTGG GCTGGAAGTG GGAAGATGAA GAGTGGTCCA CGGACCTCAA TCGGGCCGTT 
GATGAGCAAG GCTGGGAGTA CAGTATCACC ATCCCCCCGG ACCGGAAGCC GAAGCACTGG 
GTCCCCGCTG AGAAGATGTA CTACACGCAC CGTCGGCGGC GCTGGGTCCG CCTGCGCAGG 
AGGGACCTCA GCCAGATGGA GGCCCTGAGG AAGCACAGGC AGGCGGAGGC GGAGGGCGAG 
GGCTGGGAGT ACGCCTCGCT CTTTGGCTGG AAATTCCACC TGGAGTACCG CAAAACGGAT 
GCATTCCGTC GCCGCCGCTG GCGCCGCCGC ATGGAGCCGC TGGAGAAGAC GGGGCCTGCA 
GCCGTGTTTG CCCTTGAGGG GGCACTGGGT GGCGTGGTAG ATGACCGGAG TGAAGATTCC 
ATGTCCGTGT CCACCTTGAG CTTTGGCGTG AACAGACCCA CGATTTCCTG CATCTTTGAT 
CATGGGAACC GCTACCACCT GCGCTGCTAT ATGTACCAGG CTCGGGACCT GCCCTCGATG 
GACAAGGACT CTTTTTCTGA TCCCTACGCC ATCGTCTCCT TCCTGCACCA GAGCCAGAAG 
ACCGTGGTGG TGAAGAACAC TCTGAACCCC ACCTGGGACC AGACGCTCAT CTTCTATGAG 
ATTGAGATCT TTGGAGAGCC AGCCAGCATC GCCGAGCAGC CGCCCAGCAT TGTGGTGGAG 
CTGTATGACC ACGACACCTA CGGTGCAGAT GAGTTTTTGG GACGCTGCAT GTGTCAGCCC 
AGTCTGGAAC GGATGCCCCG GCTGGCCTGG TTCCCGCTGG TTAGGGGCAG CCAGCCGGCA 
GGCGAGCTGC TGGCCTCTTT TGAGCTCATC CAGAGAGAGA AGCCGGCCAT TCACCATATT 
CCTGGTTTTG AGGTGCAGGA TACATCGGGA ATCCTGGACG AGTCGGAGGA CACAGACCTG 
CCCTACCCAC CGCCTCAGAG GGAGGCCAAC ATCTACATGG TTCCCCAGAA CATCAAGCCG 
GTTCTCCAGC GCACTGCCAT AGAGATCCTG GCATGGGGCC TACGGAACAT GAAGAGTTAC 
CAGCTGGCCA GTATCTCCTC CCCCAGCCTC GTGGTGGAGT GCGGGGGCCA GACGGTGCAG 
TCCTGTGTCA TCAGGAACCT CCGGAAGAAC CCCAACTTCG ACATCTGCAC CCTCTTCATG 
GAAGTGATGC TGCCCAGAGA GGAGCTCTAC TGTCCCCCCA TCGTGATCAA GGTCATTGAT 
AACCGCCAGT TTGGCCGCCG GCCTGTGGTG GGCCAGTGTA CCATACGCTC CCTGGAGAGC 
TTCATGTGTG ACCTGTACTC CGAGGAGAGT CCGTCCCCAC AGGGCGGCCC AGATGATGTG 
AGTTTACTCA GTCCTGGGGA AGACGTGCTC ATTGACATCG ACGACAAGGA GCCCCTCATC 
CCCATACAGG AGGAGGAGTT CATCGACTGG TGGAGCAAAT TCTTTGCCTC CACAGGGGAG 
AGGGAGAAGT GCGGCTCGTA CTTGGAGAAG GGCTTTGACA CTCTAAAGGT CTATGACACA 
CAACTGGAGA ACGTGGAGGC CTTTGGGGGC CTGTCTGACT TTTGTAACAC CTTCAAGCTC 
TACCGGGGCA AGACTCAGGA GGAGACGGAG GACCCATCTG TTATCGGGGA GTTTAAGGGC 
CTCTTCAAAA TCTACCCCCT CCCGGAAGAC CCGGCCGTCC CCATGCCCCC GAGACAGTTC 
CAGCAGCTGG CTGCCCAGGG GCCTCAGGAC TGCCTGGTCC GCATCTATAT CATCCGGGCA 
TTTGGCCTGC AGCCCAAGGA CCCCAATGGG AAGTGTGATC CTTACATCAA GATCTCCATA 
GGGAAGAAAT CAGTGAGTGA CCAGGATAAT TACATTCCCT GTACCCTGGA GCCTGTGTTT 
GGAAAGATGT TCGAGCTGAC CTGCACTCTG CCTCTGGAGA AGGACCTGAA GATCACACTC 
TACGACTATG ACCTCCTCTC CAAAGATGAG AAGATCGGGG AGACAGTCAT CGACCTGGAG 
AATAGGCTGC TGTCCAAGTT CGGGGCTCGC TGCGGACTCC CACAGACCTA CTGTGTCTCT 
GGACCCAACC AGTGGAGGGA CCAGCTCCGC CCCTCCCAGC TTCTCCAGCT CACCTGCCAG 
CAGCGCAGAC TCAAGGCGCC CGTGTACCTC ACAGACCGCG TACTCTTTCA GGATAAAGAA 
TACACCATTG AGGAAATAGA GGCTGGCAGG GTCCTGAACC CACACCTGGG CCCAGTGGAA 
GAGCGTCTGG CCTTGCACGT CCTCCAGCAG CTGGGCCTGG TCCCCGAGCA TGTGGAGTCC 
CGGCCTCTTT ACAGCCCCCT GCAGCCAGAC ATTGAGCAGG GGAAGCTGCA GATGTGGATC 
GATCTGTTTC CCAAGGCCCT GGGGCGACCT GGACCTCCCT TCAACATCAC CCCACGGAGA 
GCAAGAAGGT TTTTCCTGCG TTGTATTATC TGGAACACAA AGGACGTGAT CCTGGACGAC 
CTCAGCATCA CGGGGGAGAA GATGAGTGAC ATTTACGTGA AAGGTTGGAT GGTCGGCTTT 
GAAGAGCACA AGCAGAAGAC GGACGTGCAC TACCGCTCCC TGGGAGGCGA AGGCAACTTT 
AACTGGAGGT TCATTTTCCC CTTTGACTAC CTGCCCGCTG AGCAAGTCTG CACCGTCTCT 
AAGAAGGATG CTTTCTGGAG GCTGGACAAG ACTGAGAGCA AAATCCCAGC ACAAGTGGTA 
TTTCAGATCT GGGACAATGA CAAGTTCTCC TTTGACGACT TTCTGGGCTC GTTGCAGTTG 
GATCTCAACC GCATGCCCAA GCCTGCCAAG ACAGCTGAGA AGTGCTCCTT GGACCAGCTG 
GACGACACTT TCCACCCGGA GTGGTTTGTG TCCCTTTTTG AACAGAAAAC AGTAAAGGGC 
TGGTGGCCCT GTGTGGCAGA AGACGGTGAG AAGAAGATCC TGGCGGGCAA GCTGGAAATG 
ACCTTGGAGA TCGTAGCAGA GAGTGAGCAC GAAGAGCGGC CTGCGGGTCA AGGCCGGGAT 
GAACCCAACA TGAACCCCAA GCTCGAGGAC CCAAGGCGCC CCGACACCTC CTTCCTGTGG 
TTCACCTCTC CGTACAAGAC CATGAAGTTC ATCCTGTGGC GGCGGTTCCG GTGCGCCATC 
ATCCTCTTCG TCGTTCTCGT CATCCTGCTG CTCTTCCTGG GCATCTTCCT CTATTCCTTC 
CCGAACTATG CTGCCATGAA GCTGGTGAAA CCCTTCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_019794829.1 ) cDNA ORF clone, Ailuropoda melanoleuca(giant panda) -> XP_019650388.1 Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X12, mRNA.

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CloneID	OAc89683
Clone ID Related Accession (Same CDS sequence)	XM_019794829.1
Accession Version	XM_019794829.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6315bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-27
Organism	Ailuropoda melanoleuca(giant panda)
Product	dysferlin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003217356.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ailuropoda melanoleuca Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGTGAAGAA GGACCGGCGC 
AGTGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCAGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTCCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCGG GAGGTCCTCG CCACCCCCAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCCTC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGTGGCTG ACACAGGAGG AGAGGAGGAC 
ACTGAGGACC AGGGGCTCAC GGGAGATGAG GCAGAGCCAT TACTGGATCC GAGCGGTGCC 
CCAGGCCCTG GGGCCCCCAC CACCCCGAGG AAGCCACCTT CCCATCCGCC CCCCTACCAC 
CCCGGGAGCA AGAGAAAAAG AGGTGCTCCC ACGCCCAGAA AGCTGCTGTC GGACAAACCG 
CAGGACTTCC AGATCAGGGT GCAAGTGATC GAGGGGCGCC AGCTGCCAGG GGTGAATATC 
AAGCCTGTGG TCAAAGTCAC GGCTGCCGGG CAGACCAAGA GGACACGGAT CCACAAGGGA 
AACAGCCCAC TCTTCAACGA GACTCTCTTC TTCAATGTGT TTGACTCTCC CTCGGAGCTG 
TTTGACGAGC CCATCTTTAT CACGGTGGTA GACTCCCGTT CTCTCCGGAC AGACGCTCTC 
ATTGGGGAAT TCCGGATGGA CGTGGGTACC ATCTACAGAG AGCCCCGACA CGCCTATCTG 
AGGAAATGGC TTCTCCTCTC GGACCCTGAC GATTTCTCTG CTGGGGCCAG AGGTTACCTG 
AAAGCAAGCC TCTGTGTGCT GGGGCCCGGC GATGAAGCGC CTCTGGAGAG AAAGGACCCC 
TCTGAAGACA AGGAGGACAT CGAAAACAAT CTGCTCAGGC CAACTGGCGT GGCCCTTCGA 
GGAGCCCACT TCTGCCTGAA GGTCTTCAGA GCTGAGGACT TACCACAGAT GGATGATGCC 
GTGATGGACA ACGTGAAGCA GATCTTCGGC TTTGACAGTA ACAAGAAGAA CTTGGTGGAT 
CCCTTCATGG AAGTCAGCTT TGCGGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAAACA 
GCCAACCCTC AGTGGAACCA GAGCATCACG CTGCCCGCCA TGTTTCCCTC CATGTGTGAA 
AAAATGAGGA TTCGCGTCAT GGACTGGGAC CGCCTCACCC ACAATGACAT CGTGGCCACC 
ACCTACCTGA GTATGTCGAA AATCTCCGCC CCTGGAGGAG AAATAGAAGA GGAGCCTGCA 
GGTGTGGCCA AGCCTTCTCC AGCCATGGAC CTGGACGACC ATCTGGGCTT CCTCCCCACC 
TTCGGGCCCT GCTACGTCAA CCTCTACGGC AGCCCCAGGG AGTTCACCGG CTTCCCGGAC 
CCCTACGCAG AACTCAACAT GGGCAAGGGA GAAGGTGTGG CCTACCGAGG TCGGCTTCTG 
CTCTCCCTGG AGACCAAGCT GGTGGAGCAC AGCGACCAAA AGGTGGAGGA CCTCCCTGCG 
GATGACATCC TTCGGGTGGA GAAGTATCTC CGGAGGCGTA AGTACTCCCT CTTTGCCGCC 
TTCTACTCGG CCTCCATGCT CCAGGATGTG GATGATGCCA TCCAGTTCGA GGTCAGCATC 
GGGAACTATG GCAACAAGTT CGACACCACG TGCCTGCCGC TGGCCTCTAC CACCCAGTAC 
AGCCGGGCGG TCTTTGACGG GTGCCACTAC TACTACTTGC CCTGGGGCAA CGTGAAGCCC 
GTGGTGGTGC TGTCATCCTA CTGGGAGGAC ATCAGCCACA GAGTTGAGGC TCAGAACCAG 
CTGCTCAGGA TTGCGGACCG GCTGGAAGCT GCCCTGGAGC AAGTCCACTT GGCCGTGAAG 
GCGCAGTACA ACGCCGAGGA CGTGGACTCC CTGTTGGCTC AGCTGATGGA TGAGCTCATC 
TCAGACTGCA GCCAGCCCCT AGGCGATGTC CACGAGACAC CCTCTGCCAC CCACCTGGAC 
CAGTACCTGC ACAGGCTGCG TAGCCGTCAC CTAAGCCAGA TTGCAGAGGC GGCGCTGGCC 
CTGAAGCTCG GGCACGGCGG GCTCCCGGCC GCCCTGGAGC AGGCTGAAGA CTGGCTCCTG 
CGGCTCCGTG CCCTGGCCGA GGAGCCTCAG AACAGCCTGC CCGACATCAT CATCTGGATG 
CTGCAGGGCG ACAAGCGAGT GGCATACCAG CGGGTGCCTA CACATGAGGT CCTCTTCTCC 
CGGCGTGGAG CCAGCTACTG CGGCAAGAAC TGTGGGAAGC TGCAGACGAT ATTTCTCAAA 
TATCCCATGC AGGGGGTGCC CGGAGCCCGG ATGCCCGTGC AGATACGGGT GAAGCTCTGG 
TTTGGGCTTT CTGTGGATGA GAAGGAGTTC AACCAGTTTG CTGAGGGAAA GCTGTCTGTC 
TTTGCTGAAA CCTATGAGAA CCAGACCAAA CTGGCCCTCG TTGGGAACTG GGGCACAACA 
GGCCTTACCT ACCCCAAGTT TTCTGATGTC GCGGGCAAGA TCAAGCTCCC CAAGGATAGC 
TTCCGCCCCT CAGCCGGCTG GACCTGGGCT GGAGATTGGT TTGTGTGTCC AGAGAAGACG 
CTGCTCCATG ACACCGACGC GGGCCACCTG AGTTTCGTGG CGGAGGTGTT TGAGAACCAG 
ACCCGGCTCC CTGGAGGCCA CTGGGTCTAC ATGAGTGACA ACTACACGGA TGTGAATGGG 
GAGAAGGTGC TTCCCAAGGA TGACATTGAG TGCCCGCTGG GCTGGAAGTG GGAAGATGAA 
GAGTGGTCCA CGGACCTCAA TCGGGCCGTT GATGAGCAAG GCTGGGAGTA CAGTATCACC 
ATCCCCCCGG ACCGGAAGCC GAAGCACTGG GTCCCCGCTG AGAAGATGTA CTACACGCAC 
CGTCGGCGGC GCTGGGTCCG CCTGCGCAGG AGGGACCTCA GCCAGATGGA GGCCCTGAGG 
AAGCACAGGC AGGCGGAGGC GGAGGGCGAG GGCTGGGAGT ACGCCTCGCT CTTTGGCTGG 
AAATTCCACC TGGAGTACCG CAAAACGGAT GCATTCCGTC GCCGCCGCTG GCGCCGCCGC 
ATGGAGCCGC TGGAGAAGAC GGGGCCTGCA GCCGTGTTTG CCCTTGAGGG GGCACTGGGT 
GGCGTGGTAG ATGACCGGAG TGAAGATTCC ATGTCCGTGT CCACCTTGAG CTTTGGCGTG 
AACAGACCCA CGATTTCCTG CATCTTTGAT CATGGGAACC GCTACCACCT GCGCTGCTAT 
ATGTACCAGG CTCGGGACCT GCCCTCGATG GACAAGGACT CTTTTTCTGA TCCCTACGCC 
ATCGTCTCCT TCCTGCACCA GAGCCAGAAG ACCGTGGTGG TGAAGAACAC TCTGAACCCC 
ACCTGGGACC AGACGCTCAT CTTCTATGAG ATTGAGATCT TTGGAGAGCC AGCCAGCATC 
GCCGAGCAGC CGCCCAGCAT TGTGGTGGAG CTGTATGACC ACGACACCTA CGGTGCAGAT 
GAGTTTTTGG GACGCTGCAT GTGTCAGCCC AGTCTGGAAC GGATGCCCCG GCTGGCCTGG 
TTCCCGCTGG TTAGGGGCAG CCAGCCGGCA GGCGAGCTGC TGGCCTCTTT TGAGCTCATC 
CAGAGAGAGA AGCCGGCCAT TCACCATATT CCTGGTTTTG AGGTGCAGGA TACATCGGGA 
ATCCTGGACG AGTCGGAGGA CACAGACCTG CCCTACCCAC CGCCTCAGAG GGAGGCCAAC 
ATCTACATGG TTCCCCAGAA CATCAAGCCG GTTCTCCAGC GCACTGCCAT AGAGATCCTG 
GCATGGGGCC TACGGAACAT GAAGAGTTAC CAGCTGGCCA GTATCTCCTC CCCCAGCCTC 
GTGGTGGAGT GCGGGGGCCA GACGGTGCAG TCCTGTGTCA TCAGGAACCT CCGGAAGAAC 
CCCAACTTCG ACATCTGCAC CCTCTTCATG GAAGTGATGC TGCCCAGAGA GGAGCTCTAC 
TGTCCCCCCA TCGTGATCAA GGTCATTGAT AACCGCCAGT TTGGCCGCCG GCCTGTGGTG 
GGCCAGTGTA CCATACGCTC CCTGGAGAGC TTCATGTGTG ACCTGTACTC CGAGGAGAGT 
CCGTCCCCAC AGGGCGGCCC AGATGATGTG AGTTTACTCA GTCCTGGGGA AGACGTGCTC 
ATTGACATCG ACGACAAGGA GCCCCTCATC CCCATACAGC TTACAGACGG TCTGTTGGGC 
TCTGCCCCCA CTAACATGGC ATCCTCTCCA TCCAGTCTTC ATGAGGAGGA GTTCATCGAC 
TGGTGGAGCA AATTCTTTGC CTCCACAGGG GAGAGGGAGA AGTGCGGCTC GTACTTGGAG 
AAGGGCTTTG ACACTCTAAA GGTCTATGAC ACACAACTGG AGAACGTGGA GGCCTTTGGG 
GGCCTGTCTG ACTTTTGTAA CACCTTCAAG CTCTACCGGG GCAAGACTCA GGAGGAGACG 
GAGGACCCAT CTGTTATCGG GGAGTTTAAG GGCCTCTTCA AAATCTACCC CCTCCCGGAA 
GACCCGGCCG TCCCCATGCC CCCGAGACAG TTCCAGCAGC TGGCTGCCCA GGGGCCTCAG 
GACTGCCTGG TCCGCATCTA TATCATCCGG GCATTTGGCC TGCAGCCCAA GGACCCCAAT 
GGGAAGTGTG ATCCTTACAT CAAGATCTCC ATAGGGAAGA AATCAGTGAG TGACCAGGAT 
AATTACATTC CCTGTACCCT GGAGCCTGTG TTTGGAAAGA TGTTCGAGCT GACCTGCACT 
CTGCCTCTGG AGAAGGACCT GAAGATCACA CTCTACGACT ATGACCTCCT CTCCAAAGAT 
GAGAAGATCG GGGAGACAGT CATCGACCTG GAGAATAGGC TGCTGTCCAA GTTCGGGGCT 
CGCTGCGGAC TCCCACAGAC CTACTGTGTC TCTGGACCCA ACCAGTGGAG GGACCAGCTC 
CGCCCCTCCC AGCTTCTCCA GCTCACCTGC CAGCAGCGCA GACTCAAGGC GCCCGTGTAC 
CTCACAGACC GCGTACTCTT TCAGGATAAA GAATACACCA TTGAGGAAAT AGAGGCTGGC 
AGGGTCCTGA ACCCACACCT GGGCCCAGTG GAAGAGCGTC TGGCCTTGCA CGTCCTCCAG 
CAGCTGGGCC TGGTCCCCGA GCATGTGGAG TCCCGGCCTC TTTACAGCCC CCTGCAGCCA 
GACATTGAGC AGGGGAAGCT GCAGATGTGG ATCGATCTGT TTCCCAAGGC CCTGGGGCGA 
CCTGGACCTC CCTTCAACAT CACCCCACGG AGAGCAAGAA GGTTTTTCCT GCGTTGTATT 
ATCTGGAACA CAAAGGACGT GATCCTGGAC GACCTCAGCA TCACGGGGGA GAAGATGAGT 
GACATTTACG TGAAAGGTTG GATGGTCGGC TTTGAAGAGC ACAAGCAGAA GACGGACGTG 
CACTACCGCT CCCTGGGAGG CGAAGGCAAC TTTAACTGGA GGTTCATTTT CCCCTTTGAC 
TACCTGCCCG CTGAGCAAGT CTGCACCGTC TCTAAGAAGG ATGCTTTCTG GAGGCTGGAC 
AAGACTGAGA GCAAAATCCC AGCACAAGTG GTATTTCAGA TCTGGGACAA TGACAAGTTC 
TCCTTTGACG ACTTTCTGGG CTCGTTGCAG TTGGATCTCA ACCGCATGCC CAAGCCTGCC 
AAGACAGCTG AGAAGTGCTC CTTGGACCAG CTGGACGACA CTTTCCACCC GGAGTGGTTT 
GTGTCCCTTT TTGAACAGAA AACAGTAAAG GGCTGGTGGC CCTGTGTGGC AGAAGACGGT 
GAGAAGAAGA TCCTGGCGGG CAAGCTGGAA ATGACCTTGG AGATCGTAGC AGAGAGTGAG 
CACGAAGAGC GGCCTGCGGG TCAAGGCCGG GATGAACCCA ACATGAACCC CAAGCTCGAG 
GACCCAAGGC GCCCCGACAC CTCCTTCCTG TGGTTCACCT CTCCGTACAA GACCATGAAG 
TTCATCCTGT GGCGGCGGTT CCGGTGCGCC ATCATCCTCT TCGTCGTTCT CGTCATCCTG 
CTGCTCTTCC TGGGCATCTT CCTCTATTCC TTCCCGAACT ATGCTGCCAT GAAGCTGGTG 
AAACCCTTCA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019650388.1
CDS	150..6464
Translation	MLSCLLVRASNLPSVKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEAQVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPSASLEPSPTLPDMDMVADTGGEEDTEDQGLTGDEAEPLLDPSGAPGPGAPTTPRKPPSHPPPYHPGSKRKRGAPTPRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVMDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGVAKPSPAMDLDDHLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRLLLSLETKLVEHSDQKVEDLPADDILRVEKYLRRRKYSLFAAFYSASMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADRLEAALEQVHLAVKAQYNAEDVDSLLAQLMDELISDCSQPLGDVHETPSATHLDQYLHRLRSRHLSQIAEAALALKLGHGGLPAALEQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPTHEVLFSRRGASYCGKNCGKLQTIFLKYPMQGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVAGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDTDAGHLSFVAEVFENQTRLPGGHWVYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALRKHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSMSVSTLSFGVNRPTISCIFDHGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFLGRCMCQPSLERMPRLAWFPLVRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTSGILDESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPIVIKVIDNRQFGRRPVVGQCTIRSLESFMCDLYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLTDGLLGSAPTNMASSPSSLHEEEFIDWWSKFFASTGEREKCGSYLEKGFDTLKVYDTQLENVEAFGGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAVPMPPRQFQQLAAQGPQDCLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLQLTCQQRRLKAPVYLTDRVLFQDKEYTIEEIEAGRVLNPHLGPVEERLALHVLQQLGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPAQVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEDGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFVVLVILLLFLGIFLYSFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_019794829.1
Organism	Ailuropoda melanoleuca(giant panda)
Definition	Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019794829.1

ORF Insert Sequence:

ATGCTGAGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGTGAAGAA GGACCGGCGC 
AGTGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCAGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTCCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCGG GAGGTCCTCG CCACCCCCAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCCTC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGTGGCTG ACACAGGAGG AGAGGAGGAC 
ACTGAGGACC AGGGGCTCAC GGGAGATGAG GCAGAGCCAT TACTGGATCC GAGCGGTGCC 
CCAGGCCCTG GGGCCCCCAC CACCCCGAGG AAGCCACCTT CCCATCCGCC CCCCTACCAC 
CCCGGGAGCA AGAGAAAAAG AGGTGCTCCC ACGCCCAGAA AGCTGCTGTC GGACAAACCG 
CAGGACTTCC AGATCAGGGT GCAAGTGATC GAGGGGCGCC AGCTGCCAGG GGTGAATATC 
AAGCCTGTGG TCAAAGTCAC GGCTGCCGGG CAGACCAAGA GGACACGGAT CCACAAGGGA 
AACAGCCCAC TCTTCAACGA GACTCTCTTC TTCAATGTGT TTGACTCTCC CTCGGAGCTG 
TTTGACGAGC CCATCTTTAT CACGGTGGTA GACTCCCGTT CTCTCCGGAC AGACGCTCTC 
ATTGGGGAAT TCCGGATGGA CGTGGGTACC ATCTACAGAG AGCCCCGACA CGCCTATCTG 
AGGAAATGGC TTCTCCTCTC GGACCCTGAC GATTTCTCTG CTGGGGCCAG AGGTTACCTG 
AAAGCAAGCC TCTGTGTGCT GGGGCCCGGC GATGAAGCGC CTCTGGAGAG AAAGGACCCC 
TCTGAAGACA AGGAGGACAT CGAAAACAAT CTGCTCAGGC CAACTGGCGT GGCCCTTCGA 
GGAGCCCACT TCTGCCTGAA GGTCTTCAGA GCTGAGGACT TACCACAGAT GGATGATGCC 
GTGATGGACA ACGTGAAGCA GATCTTCGGC TTTGACAGTA ACAAGAAGAA CTTGGTGGAT 
CCCTTCATGG AAGTCAGCTT TGCGGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAAACA 
GCCAACCCTC AGTGGAACCA GAGCATCACG CTGCCCGCCA TGTTTCCCTC CATGTGTGAA 
AAAATGAGGA TTCGCGTCAT GGACTGGGAC CGCCTCACCC ACAATGACAT CGTGGCCACC 
ACCTACCTGA GTATGTCGAA AATCTCCGCC CCTGGAGGAG AAATAGAAGA GGAGCCTGCA 
GGTGTGGCCA AGCCTTCTCC AGCCATGGAC CTGGACGACC ATCTGGGCTT CCTCCCCACC 
TTCGGGCCCT GCTACGTCAA CCTCTACGGC AGCCCCAGGG AGTTCACCGG CTTCCCGGAC 
CCCTACGCAG AACTCAACAT GGGCAAGGGA GAAGGTGTGG CCTACCGAGG TCGGCTTCTG 
CTCTCCCTGG AGACCAAGCT GGTGGAGCAC AGCGACCAAA AGGTGGAGGA CCTCCCTGCG 
GATGACATCC TTCGGGTGGA GAAGTATCTC CGGAGGCGTA AGTACTCCCT CTTTGCCGCC 
TTCTACTCGG CCTCCATGCT CCAGGATGTG GATGATGCCA TCCAGTTCGA GGTCAGCATC 
GGGAACTATG GCAACAAGTT CGACACCACG TGCCTGCCGC TGGCCTCTAC CACCCAGTAC 
AGCCGGGCGG TCTTTGACGG GTGCCACTAC TACTACTTGC CCTGGGGCAA CGTGAAGCCC 
GTGGTGGTGC TGTCATCCTA CTGGGAGGAC ATCAGCCACA GAGTTGAGGC TCAGAACCAG 
CTGCTCAGGA TTGCGGACCG GCTGGAAGCT GCCCTGGAGC AAGTCCACTT GGCCGTGAAG 
GCGCAGTACA ACGCCGAGGA CGTGGACTCC CTGTTGGCTC AGCTGATGGA TGAGCTCATC 
TCAGACTGCA GCCAGCCCCT AGGCGATGTC CACGAGACAC CCTCTGCCAC CCACCTGGAC 
CAGTACCTGC ACAGGCTGCG TAGCCGTCAC CTAAGCCAGA TTGCAGAGGC GGCGCTGGCC 
CTGAAGCTCG GGCACGGCGG GCTCCCGGCC GCCCTGGAGC AGGCTGAAGA CTGGCTCCTG 
CGGCTCCGTG CCCTGGCCGA GGAGCCTCAG AACAGCCTGC CCGACATCAT CATCTGGATG 
CTGCAGGGCG ACAAGCGAGT GGCATACCAG CGGGTGCCTA CACATGAGGT CCTCTTCTCC 
CGGCGTGGAG CCAGCTACTG CGGCAAGAAC TGTGGGAAGC TGCAGACGAT ATTTCTCAAA 
TATCCCATGC AGGGGGTGCC CGGAGCCCGG ATGCCCGTGC AGATACGGGT GAAGCTCTGG 
TTTGGGCTTT CTGTGGATGA GAAGGAGTTC AACCAGTTTG CTGAGGGAAA GCTGTCTGTC 
TTTGCTGAAA CCTATGAGAA CCAGACCAAA CTGGCCCTCG TTGGGAACTG GGGCACAACA 
GGCCTTACCT ACCCCAAGTT TTCTGATGTC GCGGGCAAGA TCAAGCTCCC CAAGGATAGC 
TTCCGCCCCT CAGCCGGCTG GACCTGGGCT GGAGATTGGT TTGTGTGTCC AGAGAAGACG 
CTGCTCCATG ACACCGACGC GGGCCACCTG AGTTTCGTGG CGGAGGTGTT TGAGAACCAG 
ACCCGGCTCC CTGGAGGCCA CTGGGTCTAC ATGAGTGACA ACTACACGGA TGTGAATGGG 
GAGAAGGTGC TTCCCAAGGA TGACATTGAG TGCCCGCTGG GCTGGAAGTG GGAAGATGAA 
GAGTGGTCCA CGGACCTCAA TCGGGCCGTT GATGAGCAAG GCTGGGAGTA CAGTATCACC 
ATCCCCCCGG ACCGGAAGCC GAAGCACTGG GTCCCCGCTG AGAAGATGTA CTACACGCAC 
CGTCGGCGGC GCTGGGTCCG CCTGCGCAGG AGGGACCTCA GCCAGATGGA GGCCCTGAGG 
AAGCACAGGC AGGCGGAGGC GGAGGGCGAG GGCTGGGAGT ACGCCTCGCT CTTTGGCTGG 
AAATTCCACC TGGAGTACCG CAAAACGGAT GCATTCCGTC GCCGCCGCTG GCGCCGCCGC 
ATGGAGCCGC TGGAGAAGAC GGGGCCTGCA GCCGTGTTTG CCCTTGAGGG GGCACTGGGT 
GGCGTGGTAG ATGACCGGAG TGAAGATTCC ATGTCCGTGT CCACCTTGAG CTTTGGCGTG 
AACAGACCCA CGATTTCCTG CATCTTTGAT CATGGGAACC GCTACCACCT GCGCTGCTAT 
ATGTACCAGG CTCGGGACCT GCCCTCGATG GACAAGGACT CTTTTTCTGA TCCCTACGCC 
ATCGTCTCCT TCCTGCACCA GAGCCAGAAG ACCGTGGTGG TGAAGAACAC TCTGAACCCC 
ACCTGGGACC AGACGCTCAT CTTCTATGAG ATTGAGATCT TTGGAGAGCC AGCCAGCATC 
GCCGAGCAGC CGCCCAGCAT TGTGGTGGAG CTGTATGACC ACGACACCTA CGGTGCAGAT 
GAGTTTTTGG GACGCTGCAT GTGTCAGCCC AGTCTGGAAC GGATGCCCCG GCTGGCCTGG 
TTCCCGCTGG TTAGGGGCAG CCAGCCGGCA GGCGAGCTGC TGGCCTCTTT TGAGCTCATC 
CAGAGAGAGA AGCCGGCCAT TCACCATATT CCTGGTTTTG AGGTGCAGGA TACATCGGGA 
ATCCTGGACG AGTCGGAGGA CACAGACCTG CCCTACCCAC CGCCTCAGAG GGAGGCCAAC 
ATCTACATGG TTCCCCAGAA CATCAAGCCG GTTCTCCAGC GCACTGCCAT AGAGATCCTG 
GCATGGGGCC TACGGAACAT GAAGAGTTAC CAGCTGGCCA GTATCTCCTC CCCCAGCCTC 
GTGGTGGAGT GCGGGGGCCA GACGGTGCAG TCCTGTGTCA TCAGGAACCT CCGGAAGAAC 
CCCAACTTCG ACATCTGCAC CCTCTTCATG GAAGTGATGC TGCCCAGAGA GGAGCTCTAC 
TGTCCCCCCA TCGTGATCAA GGTCATTGAT AACCGCCAGT TTGGCCGCCG GCCTGTGGTG 
GGCCAGTGTA CCATACGCTC CCTGGAGAGC TTCATGTGTG ACCTGTACTC CGAGGAGAGT 
CCGTCCCCAC AGGGCGGCCC AGATGATGTG AGTTTACTCA GTCCTGGGGA AGACGTGCTC 
ATTGACATCG ACGACAAGGA GCCCCTCATC CCCATACAGC TTACAGACGG TCTGTTGGGC 
TCTGCCCCCA CTAACATGGC ATCCTCTCCA TCCAGTCTTC ATGAGGAGGA GTTCATCGAC 
TGGTGGAGCA AATTCTTTGC CTCCACAGGG GAGAGGGAGA AGTGCGGCTC GTACTTGGAG 
AAGGGCTTTG ACACTCTAAA GGTCTATGAC ACACAACTGG AGAACGTGGA GGCCTTTGGG 
GGCCTGTCTG ACTTTTGTAA CACCTTCAAG CTCTACCGGG GCAAGACTCA GGAGGAGACG 
GAGGACCCAT CTGTTATCGG GGAGTTTAAG GGCCTCTTCA AAATCTACCC CCTCCCGGAA 
GACCCGGCCG TCCCCATGCC CCCGAGACAG TTCCAGCAGC TGGCTGCCCA GGGGCCTCAG 
GACTGCCTGG TCCGCATCTA TATCATCCGG GCATTTGGCC TGCAGCCCAA GGACCCCAAT 
GGGAAGTGTG ATCCTTACAT CAAGATCTCC ATAGGGAAGA AATCAGTGAG TGACCAGGAT 
AATTACATTC CCTGTACCCT GGAGCCTGTG TTTGGAAAGA TGTTCGAGCT GACCTGCACT 
CTGCCTCTGG AGAAGGACCT GAAGATCACA CTCTACGACT ATGACCTCCT CTCCAAAGAT 
GAGAAGATCG GGGAGACAGT CATCGACCTG GAGAATAGGC TGCTGTCCAA GTTCGGGGCT 
CGCTGCGGAC TCCCACAGAC CTACTGTGTC TCTGGACCCA ACCAGTGGAG GGACCAGCTC 
CGCCCCTCCC AGCTTCTCCA GCTCACCTGC CAGCAGCGCA GACTCAAGGC GCCCGTGTAC 
CTCACAGACC GCGTACTCTT TCAGGATAAA GAATACACCA TTGAGGAAAT AGAGGCTGGC 
AGGGTCCTGA ACCCACACCT GGGCCCAGTG GAAGAGCGTC TGGCCTTGCA CGTCCTCCAG 
CAGCTGGGCC TGGTCCCCGA GCATGTGGAG TCCCGGCCTC TTTACAGCCC CCTGCAGCCA 
GACATTGAGC AGGGGAAGCT GCAGATGTGG ATCGATCTGT TTCCCAAGGC CCTGGGGCGA 
CCTGGACCTC CCTTCAACAT CACCCCACGG AGAGCAAGAA GGTTTTTCCT GCGTTGTATT 
ATCTGGAACA CAAAGGACGT GATCCTGGAC GACCTCAGCA TCACGGGGGA GAAGATGAGT 
GACATTTACG TGAAAGGTTG GATGGTCGGC TTTGAAGAGC ACAAGCAGAA GACGGACGTG 
CACTACCGCT CCCTGGGAGG CGAAGGCAAC TTTAACTGGA GGTTCATTTT CCCCTTTGAC 
TACCTGCCCG CTGAGCAAGT CTGCACCGTC TCTAAGAAGG ATGCTTTCTG GAGGCTGGAC 
AAGACTGAGA GCAAAATCCC AGCACAAGTG GTATTTCAGA TCTGGGACAA TGACAAGTTC 
TCCTTTGACG ACTTTCTGGG CTCGTTGCAG TTGGATCTCA ACCGCATGCC CAAGCCTGCC 
AAGACAGCTG AGAAGTGCTC CTTGGACCAG CTGGACGACA CTTTCCACCC GGAGTGGTTT 
GTGTCCCTTT TTGAACAGAA AACAGTAAAG GGCTGGTGGC CCTGTGTGGC AGAAGACGGT 
GAGAAGAAGA TCCTGGCGGG CAAGCTGGAA ATGACCTTGG AGATCGTAGC AGAGAGTGAG 
CACGAAGAGC GGCCTGCGGG TCAAGGCCGG GATGAACCCA ACATGAACCC CAAGCTCGAG 
GACCCAAGGC GCCCCGACAC CTCCTTCCTG TGGTTCACCT CTCCGTACAA GACCATGAAG 
TTCATCCTGT GGCGGCGGTT CCGGTGCGCC ATCATCCTCT TCGTCGTTCT CGTCATCCTG 
CTGCTCTTCC TGGGCATCTT CCTCTATTCC TTCCCGAACT ATGCTGCCAT GAAGCTGGTG 
AAACCCTTCA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_019794830.1 ) cDNA ORF clone, Ailuropoda melanoleuca(giant panda) -> XP_019650389.1 Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X13, mRNA.

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CloneID	OAc89684
Clone ID Related Accession (Same CDS sequence)	XM_019794830.1
Accession Version	XM_019794830.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6312bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-27
Organism	Ailuropoda melanoleuca(giant panda)
Product	dysferlin isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003217356.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ailuropoda melanoleuca Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTATGCCGAG AATGTCCACA CCCCGGACAC CGACATCAGC 
GATGCCTACT GCTCTGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CAGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGAGCTCCG AGCTCCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
TTAGGGGAAG CCCAAGTCCC ACTCCGGGAG GTCCTCGCCA CCCCCAGTCT GTCTGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CGCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCCTCTGC ATCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATATG GTGGCTGACA CAGGAGGAGA GGAGGACACT 
GAGGACCAGG GGCTCACGGG AGATGAGGCA GAGCCATTAC TGGATCCGAG CGGTGCCCCA 
GGCCCTGGGG CCCCCACCAC CCCGAGGAAG CCACCTTCCC ATCCGCCCCC CTACCACCCC 
GGGAGCAAGA GAAAAAGAGG TGCTCCCACG CCCAGAAAGC TGCTGTCGGA CAAACCGCAG 
GACTTCCAGA TCAGGGTGCA AGTGATCGAG GGGCGCCAGC TGCCAGGGGT GAATATCAAG 
CCTGTGGTCA AAGTCACGGC TGCCGGGCAG ACCAAGAGGA CACGGATCCA CAAGGGAAAC 
AGCCCACTCT TCAACGAGAC TCTCTTCTTC AATGTGTTTG ACTCTCCCTC GGAGCTGTTT 
GACGAGCCCA TCTTTATCAC GGTGGTAGAC TCCCGTTCTC TCCGGACAGA CGCTCTCATT 
GGGGAATTCC GGATGGACGT GGGTACCATC TACAGAGAGC CCCGACACGC CTATCTGAGG 
AAATGGCTTC TCCTCTCGGA CCCTGACGAT TTCTCTGCTG GGGCCAGAGG TTACCTGAAA 
GCAAGCCTCT GTGTGCTGGG GCCCGGCGAT GAAGCGCCTC TGGAGAGAAA GGACCCCTCT 
GAAGACAAGG AGGACATCGA AAACAATCTG CTCAGGCCAA CTGGCGTGGC CCTTCGAGGA 
GCCCACTTCT GCCTGAAGGT CTTCAGAGCT GAGGACTTAC CACAGATGGA TGATGCCGTG 
ATGGACAACG TGAAGCAGAT CTTCGGCTTT GACAGTAACA AGAAGAACTT GGTGGATCCC 
TTCATGGAAG TCAGCTTTGC GGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAAACAGCC 
AACCCTCAGT GGAACCAGAG CATCACGCTG CCCGCCATGT TTCCCTCCAT GTGTGAAAAA 
ATGAGGATTC GCGTCATGGA CTGGGACCGC CTCACCCACA ATGACATCGT GGCCACCACC 
TACCTGAGTA TGTCGAAAAT CTCCGCCCCT GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT 
GTGGCCAAGC CTTCTCCAGC CATGGACCTG GACGACCATC TGGGCTTCCT CCCCACCTTC 
GGGCCCTGCT ACGTCAACCT CTACGGCAGC CCCAGGGAGT TCACCGGCTT CCCGGACCCC 
TACGCAGAAC TCAACATGGG CAAGGGAGAA GGTGTGGCCT ACCGAGGTCG GCTTCTGCTC 
TCCCTGGAGA CCAAGCTGGT GGAGCACAGC GACCAAAAGG TGGAGGACCT CCCTGCGGAT 
GACATCCTTC GGGTGGAGAA GTATCTCCGG AGGCGTAAGT ACTCCCTCTT TGCCGCCTTC 
TACTCGGCCT CCATGCTCCA GGATGTGGAT GATGCCATCC AGTTCGAGGT CAGCATCGGG 
AACTATGGCA ACAAGTTCGA CACCACGTGC CTGCCGCTGG CCTCTACCAC CCAGTACAGC 
CGGGCGGTCT TTGACGGGTG CCACTACTAC TACTTGCCCT GGGGCAACGT GAAGCCCGTG 
GTGGTGCTGT CATCCTACTG GGAGGACATC AGCCACAGAG TTGAGGCTCA GAACCAGCTG 
CTCAGGATTG CGGACCGGCT GGAAGCTGCC CTGGAGCAAG TCCACTTGGC CGTGAAGGCG 
CAGTACAACG CCGAGGACGT GGACTCCCTG TTGGCTCAGC TGATGGATGA GCTCATCTCA 
GACTGCAGCC AGCCCCTAGG CGATGTCCAC GAGACACCCT CTGCCACCCA CCTGGACCAG 
TACCTGCACA GGCTGCGTAG CCGTCACCTA AGCCAGATTG CAGAGGCGGC GCTGGCCCTG 
AAGCTCGGGC ACGGCGGGCT CCCGGCCGCC CTGGAGCAGG CTGAAGACTG GCTCCTGCGG 
CTCCGTGCCC TGGCCGAGGA GCCTCAGAAC AGCCTGCCCG ACATCATCAT CTGGATGCTG 
CAGGGCGACA AGCGAGTGGC ATACCAGCGG GTGCCTACAC ATGAGGTCCT CTTCTCCCGG 
CGTGGAGCCA GCTACTGCGG CAAGAACTGT GGGAAGCTGC AGACGATATT TCTCAAATAT 
CCCATGCAGG GGGTGCCCGG AGCCCGGATG CCCGTGCAGA TACGGGTGAA GCTCTGGTTT 
GGGCTTTCTG TGGATGAGAA GGAGTTCAAC CAGTTTGCTG AGGGAAAGCT GTCTGTCTTT 
GCTGAAACCT ATGAGAACCA GACCAAACTG GCCCTCGTTG GGAACTGGGG CACAACAGGC 
CTTACCTACC CCAAGTTTTC TGATGTCGCG GGCAAGATCA AGCTCCCCAA GGATAGCTTC 
CGCCCCTCAG CCGGCTGGAC CTGGGCTGGA GATTGGTTTG TGTGTCCAGA GAAGACGCTG 
CTCCATGACA CCGACGCGGG CCACCTGAGT TTCGTGGCGG AGGTGTTTGA GAACCAGACC 
CGGCTCCCTG GAGGCCACTG GGTCTACATG AGTGACAACT ACACGGATGT GAATGGGGAG 
AAGGTGCTTC CCAAGGATGA CATTGAGTGC CCGCTGGGCT GGAAGTGGGA AGATGAAGAG 
TGGTCCACGG ACCTCAATCG GGCCGTTGAT GAGCAAGGCT GGGAGTACAG TATCACCATC 
CCCCCGGACC GGAAGCCGAA GCACTGGGTC CCCGCTGAGA AGATGTACTA CACGCACCGT 
CGGCGGCGCT GGGTCCGCCT GCGCAGGAGG GACCTCAGCC AGATGGAGGC CCTGAGGAAG 
CACAGGCAGG CGGAGGCGGA GGGCGAGGGC TGGGAGTACG CCTCGCTCTT TGGCTGGAAA 
TTCCACCTGG AGTACCGCAA AACGGATGCA TTCCGTCGCC GCCGCTGGCG CCGCCGCATG 
GAGCCGCTGG AGAAGACGGG GCCTGCAGCC GTGTTTGCCC TTGAGGGGGC ACTGGGTGGC 
GTGGTAGATG ACCGGAGTGA AGATTCCATG TCCGTGTCCA CCTTGAGCTT TGGCGTGAAC 
AGACCCACGA TTTCCTGCAT CTTTGATCAT GGGAACCGCT ACCACCTGCG CTGCTATATG 
TACCAGGCTC GGGACCTGCC CTCGATGGAC AAGGACTCTT TTTCTGATCC CTACGCCATC 
GTCTCCTTCC TGCACCAGAG CCAGAAGACC GTGGTGGTGA AGAACACTCT GAACCCCACC 
TGGGACCAGA CGCTCATCTT CTATGAGATT GAGATCTTTG GAGAGCCAGC CAGCATCGCC 
GAGCAGCCGC CCAGCATTGT GGTGGAGCTG TATGACCACG ACACCTACGG TGCAGATGAG 
TTTTTGGGAC GCTGCATGTG TCAGCCCAGT CTGGAACGGA TGCCCCGGCT GGCCTGGTTC 
CCGCTGGTTA GGGGCAGCCA GCCGGCAGGC GAGCTGCTGG CCTCTTTTGA GCTCATCCAG 
AGAGAGAAGC CGGCCATTCA CCATATTCCT GGTTTTGAGG TGCAGGATAC ATCGGGAATC 
CTGGACGAGT CGGAGGACAC AGACCTGCCC TACCCACCGC CTCAGAGGGA GGCCAACATC 
TACATGGTTC CCCAGAACAT CAAGCCGGTT CTCCAGCGCA CTGCCATAGA GATCCTGGCA 
TGGGGCCTAC GGAACATGAA GAGTTACCAG CTGGCCAGTA TCTCCTCCCC CAGCCTCGTG 
GTGGAGTGCG GGGGCCAGAC GGTGCAGTCC TGTGTCATCA GGAACCTCCG GAAGAACCCC 
AACTTCGACA TCTGCACCCT CTTCATGGAA GTGATGCTGC CCAGAGAGGA GCTCTACTGT 
CCCCCCATCG TGATCAAGGT CATTGATAAC CGCCAGTTTG GCCGCCGGCC TGTGGTGGGC 
CAGTGTACCA TACGCTCCCT GGAGAGCTTC ATGTGTGACC TGTACTCCGA GGAGAGTCCG 
TCCCCACAGG GCGGCCCAGA TGATGTGAGT TTACTCAGTC CTGGGGAAGA CGTGCTCATT 
GACATCGACG ACAAGGAGCC CCTCATCCCC ATACAGCTTA CAGACGGTCT GTTGGGCTCT 
GCCCCCACTA ACATGGCATC CTCTCCATCC AGTCTTCATG AGGAGGAGTT CATCGACTGG 
TGGAGCAAAT TCTTTGCCTC CACAGGGGAG AGGGAGAAGT GCGGCTCGTA CTTGGAGAAG 
GGCTTTGACA CTCTAAAGGT CTATGACACA CAACTGGAGA ACGTGGAGGC CTTTGGGGGC 
CTGTCTGACT TTTGTAACAC CTTCAAGCTC TACCGGGGCA AGACTCAGGA GGAGACGGAG 
GACCCATCTG TTATCGGGGA GTTTAAGGGC CTCTTCAAAA TCTACCCCCT CCCGGAAGAC 
CCGGCCGTCC CCATGCCCCC GAGACAGTTC CAGCAGCTGG CTGCCCAGGG GCCTCAGGAC 
TGCCTGGTCC GCATCTATAT CATCCGGGCA TTTGGCCTGC AGCCCAAGGA CCCCAATGGG 
AAGTGTGATC CTTACATCAA GATCTCCATA GGGAAGAAAT CAGTGAGTGA CCAGGATAAT 
TACATTCCCT GTACCCTGGA GCCTGTGTTT GGAAAGATGT TCGAGCTGAC CTGCACTCTG 
CCTCTGGAGA AGGACCTGAA GATCACACTC TACGACTATG ACCTCCTCTC CAAAGATGAG 
AAGATCGGGG AGACAGTCAT CGACCTGGAG AATAGGCTGC TGTCCAAGTT CGGGGCTCGC 
TGCGGACTCC CACAGACCTA CTGTGTCTCT GGACCCAACC AGTGGAGGGA CCAGCTCCGC 
CCCTCCCAGC TTCTCCAGCT CACCTGCCAG CAGCGCAGAC TCAAGGCGCC CGTGTACCTC 
ACAGACCGCG TACTCTTTCA GGATAAAGAA TACACCATTG AGGAAATAGA GGCTGGCAGG 
GTCCTGAACC CACACCTGGG CCCAGTGGAA GAGCGTCTGG CCTTGCACGT CCTCCAGCAG 
CTGGGCCTGG TCCCCGAGCA TGTGGAGTCC CGGCCTCTTT ACAGCCCCCT GCAGCCAGAC 
ATTGAGCAGG GGAAGCTGCA GATGTGGATC GATCTGTTTC CCAAGGCCCT GGGGCGACCT 
GGACCTCCCT TCAACATCAC CCCACGGAGA GCAAGAAGGT TTTTCCTGCG TTGTATTATC 
TGGAACACAA AGGACGTGAT CCTGGACGAC CTCAGCATCA CGGGGGAGAA GATGAGTGAC 
ATTTACGTGA AAGGTTGGAT GGTCGGCTTT GAAGAGCACA AGCAGAAGAC GGACGTGCAC 
TACCGCTCCC TGGGAGGCGA AGGCAACTTT AACTGGAGGT TCATTTTCCC CTTTGACTAC 
CTGCCCGCTG AGCAAGTCTG CACCGTCTCT AAGAAGGATG CTTTCTGGAG GCTGGACAAG 
ACTGAGAGCA AAATCCCAGC ACAAGTGGTA TTTCAGATCT GGGACAATGA CAAGTTCTCC 
TTTGACGACT TTCTGGGCTC GTTGCAGTTG GATCTCAACC GCATGCCCAA GCCTGCCAAG 
ACAGCTGAGA AGTGCTCCTT GGACCAGCTG GACGACACTT TCCACCCGGA GTGGTTTGTG 
TCCCTTTTTG AACAGAAAAC AGTAAAGGGC TGGTGGCCCT GTGTGGCAGA AGACGGTGAG 
AAGAAGATCC TGGCGGGCAA GCTGGAAATG ACCTTGGAGA TCGTAGCAGA GAGTGAGCAC 
GAAGAGCGGC CTGCGGGTCA AGGCCGGGAT GAACCCAACA TGAACCCCAA GCTCGAGGAC 
CCAAGGCGCC CCGACACCTC CTTCCTGTGG TTCACCTCTC CGTACAAGAC CATGAAGTTC 
ATCCTGTGGC GGCGGTTCCG GTGCGCCATC ATCCTCTTCG TCGTTCTCGT CATCCTGCTG 
CTCTTCCTGG GCATCTTCCT CTATTCCTTC CCGAACTATG CTGCCATGAA GCTGGTGAAA 
CCCTTCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019650389.1
CDS	487..6798
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEAQVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPSASLEPSPTLPDMDMVADTGGEEDTEDQGLTGDEAEPLLDPSGAPGPGAPTTPRKPPSHPPPYHPGSKRKRGAPTPRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVMDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGVAKPSPAMDLDDHLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRLLLSLETKLVEHSDQKVEDLPADDILRVEKYLRRRKYSLFAAFYSASMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADRLEAALEQVHLAVKAQYNAEDVDSLLAQLMDELISDCSQPLGDVHETPSATHLDQYLHRLRSRHLSQIAEAALALKLGHGGLPAALEQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPTHEVLFSRRGASYCGKNCGKLQTIFLKYPMQGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVAGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDTDAGHLSFVAEVFENQTRLPGGHWVYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALRKHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSMSVSTLSFGVNRPTISCIFDHGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFLGRCMCQPSLERMPRLAWFPLVRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTSGILDESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPIVIKVIDNRQFGRRPVVGQCTIRSLESFMCDLYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLTDGLLGSAPTNMASSPSSLHEEEFIDWWSKFFASTGEREKCGSYLEKGFDTLKVYDTQLENVEAFGGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAVPMPPRQFQQLAAQGPQDCLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLQLTCQQRRLKAPVYLTDRVLFQDKEYTIEEIEAGRVLNPHLGPVEERLALHVLQQLGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPAQVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEDGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFVVLVILLLFLGIFLYSFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_019794830.1
Organism	Ailuropoda melanoleuca(giant panda)
Definition	Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019794830.1

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCCGAG AATGTCCACA CCCCGGACAC CGACATCAGC 
GATGCCTACT GCTCTGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CAGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGAGCTCCG AGCTCCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
TTAGGGGAAG CCCAAGTCCC ACTCCGGGAG GTCCTCGCCA CCCCCAGTCT GTCTGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CGCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCCTCTGC ATCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATATG GTGGCTGACA CAGGAGGAGA GGAGGACACT 
GAGGACCAGG GGCTCACGGG AGATGAGGCA GAGCCATTAC TGGATCCGAG CGGTGCCCCA 
GGCCCTGGGG CCCCCACCAC CCCGAGGAAG CCACCTTCCC ATCCGCCCCC CTACCACCCC 
GGGAGCAAGA GAAAAAGAGG TGCTCCCACG CCCAGAAAGC TGCTGTCGGA CAAACCGCAG 
GACTTCCAGA TCAGGGTGCA AGTGATCGAG GGGCGCCAGC TGCCAGGGGT GAATATCAAG 
CCTGTGGTCA AAGTCACGGC TGCCGGGCAG ACCAAGAGGA CACGGATCCA CAAGGGAAAC 
AGCCCACTCT TCAACGAGAC TCTCTTCTTC AATGTGTTTG ACTCTCCCTC GGAGCTGTTT 
GACGAGCCCA TCTTTATCAC GGTGGTAGAC TCCCGTTCTC TCCGGACAGA CGCTCTCATT 
GGGGAATTCC GGATGGACGT GGGTACCATC TACAGAGAGC CCCGACACGC CTATCTGAGG 
AAATGGCTTC TCCTCTCGGA CCCTGACGAT TTCTCTGCTG GGGCCAGAGG TTACCTGAAA 
GCAAGCCTCT GTGTGCTGGG GCCCGGCGAT GAAGCGCCTC TGGAGAGAAA GGACCCCTCT 
GAAGACAAGG AGGACATCGA AAACAATCTG CTCAGGCCAA CTGGCGTGGC CCTTCGAGGA 
GCCCACTTCT GCCTGAAGGT CTTCAGAGCT GAGGACTTAC CACAGATGGA TGATGCCGTG 
ATGGACAACG TGAAGCAGAT CTTCGGCTTT GACAGTAACA AGAAGAACTT GGTGGATCCC 
TTCATGGAAG TCAGCTTTGC GGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAAACAGCC 
AACCCTCAGT GGAACCAGAG CATCACGCTG CCCGCCATGT TTCCCTCCAT GTGTGAAAAA 
ATGAGGATTC GCGTCATGGA CTGGGACCGC CTCACCCACA ATGACATCGT GGCCACCACC 
TACCTGAGTA TGTCGAAAAT CTCCGCCCCT GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT 
GTGGCCAAGC CTTCTCCAGC CATGGACCTG GACGACCATC TGGGCTTCCT CCCCACCTTC 
GGGCCCTGCT ACGTCAACCT CTACGGCAGC CCCAGGGAGT TCACCGGCTT CCCGGACCCC 
TACGCAGAAC TCAACATGGG CAAGGGAGAA GGTGTGGCCT ACCGAGGTCG GCTTCTGCTC 
TCCCTGGAGA CCAAGCTGGT GGAGCACAGC GACCAAAAGG TGGAGGACCT CCCTGCGGAT 
GACATCCTTC GGGTGGAGAA GTATCTCCGG AGGCGTAAGT ACTCCCTCTT TGCCGCCTTC 
TACTCGGCCT CCATGCTCCA GGATGTGGAT GATGCCATCC AGTTCGAGGT CAGCATCGGG 
AACTATGGCA ACAAGTTCGA CACCACGTGC CTGCCGCTGG CCTCTACCAC CCAGTACAGC 
CGGGCGGTCT TTGACGGGTG CCACTACTAC TACTTGCCCT GGGGCAACGT GAAGCCCGTG 
GTGGTGCTGT CATCCTACTG GGAGGACATC AGCCACAGAG TTGAGGCTCA GAACCAGCTG 
CTCAGGATTG CGGACCGGCT GGAAGCTGCC CTGGAGCAAG TCCACTTGGC CGTGAAGGCG 
CAGTACAACG CCGAGGACGT GGACTCCCTG TTGGCTCAGC TGATGGATGA GCTCATCTCA 
GACTGCAGCC AGCCCCTAGG CGATGTCCAC GAGACACCCT CTGCCACCCA CCTGGACCAG 
TACCTGCACA GGCTGCGTAG CCGTCACCTA AGCCAGATTG CAGAGGCGGC GCTGGCCCTG 
AAGCTCGGGC ACGGCGGGCT CCCGGCCGCC CTGGAGCAGG CTGAAGACTG GCTCCTGCGG 
CTCCGTGCCC TGGCCGAGGA GCCTCAGAAC AGCCTGCCCG ACATCATCAT CTGGATGCTG 
CAGGGCGACA AGCGAGTGGC ATACCAGCGG GTGCCTACAC ATGAGGTCCT CTTCTCCCGG 
CGTGGAGCCA GCTACTGCGG CAAGAACTGT GGGAAGCTGC AGACGATATT TCTCAAATAT 
CCCATGCAGG GGGTGCCCGG AGCCCGGATG CCCGTGCAGA TACGGGTGAA GCTCTGGTTT 
GGGCTTTCTG TGGATGAGAA GGAGTTCAAC CAGTTTGCTG AGGGAAAGCT GTCTGTCTTT 
GCTGAAACCT ATGAGAACCA GACCAAACTG GCCCTCGTTG GGAACTGGGG CACAACAGGC 
CTTACCTACC CCAAGTTTTC TGATGTCGCG GGCAAGATCA AGCTCCCCAA GGATAGCTTC 
CGCCCCTCAG CCGGCTGGAC CTGGGCTGGA GATTGGTTTG TGTGTCCAGA GAAGACGCTG 
CTCCATGACA CCGACGCGGG CCACCTGAGT TTCGTGGCGG AGGTGTTTGA GAACCAGACC 
CGGCTCCCTG GAGGCCACTG GGTCTACATG AGTGACAACT ACACGGATGT GAATGGGGAG 
AAGGTGCTTC CCAAGGATGA CATTGAGTGC CCGCTGGGCT GGAAGTGGGA AGATGAAGAG 
TGGTCCACGG ACCTCAATCG GGCCGTTGAT GAGCAAGGCT GGGAGTACAG TATCACCATC 
CCCCCGGACC GGAAGCCGAA GCACTGGGTC CCCGCTGAGA AGATGTACTA CACGCACCGT 
CGGCGGCGCT GGGTCCGCCT GCGCAGGAGG GACCTCAGCC AGATGGAGGC CCTGAGGAAG 
CACAGGCAGG CGGAGGCGGA GGGCGAGGGC TGGGAGTACG CCTCGCTCTT TGGCTGGAAA 
TTCCACCTGG AGTACCGCAA AACGGATGCA TTCCGTCGCC GCCGCTGGCG CCGCCGCATG 
GAGCCGCTGG AGAAGACGGG GCCTGCAGCC GTGTTTGCCC TTGAGGGGGC ACTGGGTGGC 
GTGGTAGATG ACCGGAGTGA AGATTCCATG TCCGTGTCCA CCTTGAGCTT TGGCGTGAAC 
AGACCCACGA TTTCCTGCAT CTTTGATCAT GGGAACCGCT ACCACCTGCG CTGCTATATG 
TACCAGGCTC GGGACCTGCC CTCGATGGAC AAGGACTCTT TTTCTGATCC CTACGCCATC 
GTCTCCTTCC TGCACCAGAG CCAGAAGACC GTGGTGGTGA AGAACACTCT GAACCCCACC 
TGGGACCAGA CGCTCATCTT CTATGAGATT GAGATCTTTG GAGAGCCAGC CAGCATCGCC 
GAGCAGCCGC CCAGCATTGT GGTGGAGCTG TATGACCACG ACACCTACGG TGCAGATGAG 
TTTTTGGGAC GCTGCATGTG TCAGCCCAGT CTGGAACGGA TGCCCCGGCT GGCCTGGTTC 
CCGCTGGTTA GGGGCAGCCA GCCGGCAGGC GAGCTGCTGG CCTCTTTTGA GCTCATCCAG 
AGAGAGAAGC CGGCCATTCA CCATATTCCT GGTTTTGAGG TGCAGGATAC ATCGGGAATC 
CTGGACGAGT CGGAGGACAC AGACCTGCCC TACCCACCGC CTCAGAGGGA GGCCAACATC 
TACATGGTTC CCCAGAACAT CAAGCCGGTT CTCCAGCGCA CTGCCATAGA GATCCTGGCA 
TGGGGCCTAC GGAACATGAA GAGTTACCAG CTGGCCAGTA TCTCCTCCCC CAGCCTCGTG 
GTGGAGTGCG GGGGCCAGAC GGTGCAGTCC TGTGTCATCA GGAACCTCCG GAAGAACCCC 
AACTTCGACA TCTGCACCCT CTTCATGGAA GTGATGCTGC CCAGAGAGGA GCTCTACTGT 
CCCCCCATCG TGATCAAGGT CATTGATAAC CGCCAGTTTG GCCGCCGGCC TGTGGTGGGC 
CAGTGTACCA TACGCTCCCT GGAGAGCTTC ATGTGTGACC TGTACTCCGA GGAGAGTCCG 
TCCCCACAGG GCGGCCCAGA TGATGTGAGT TTACTCAGTC CTGGGGAAGA CGTGCTCATT 
GACATCGACG ACAAGGAGCC CCTCATCCCC ATACAGCTTA CAGACGGTCT GTTGGGCTCT 
GCCCCCACTA ACATGGCATC CTCTCCATCC AGTCTTCATG AGGAGGAGTT CATCGACTGG 
TGGAGCAAAT TCTTTGCCTC CACAGGGGAG AGGGAGAAGT GCGGCTCGTA CTTGGAGAAG 
GGCTTTGACA CTCTAAAGGT CTATGACACA CAACTGGAGA ACGTGGAGGC CTTTGGGGGC 
CTGTCTGACT TTTGTAACAC CTTCAAGCTC TACCGGGGCA AGACTCAGGA GGAGACGGAG 
GACCCATCTG TTATCGGGGA GTTTAAGGGC CTCTTCAAAA TCTACCCCCT CCCGGAAGAC 
CCGGCCGTCC CCATGCCCCC GAGACAGTTC CAGCAGCTGG CTGCCCAGGG GCCTCAGGAC 
TGCCTGGTCC GCATCTATAT CATCCGGGCA TTTGGCCTGC AGCCCAAGGA CCCCAATGGG 
AAGTGTGATC CTTACATCAA GATCTCCATA GGGAAGAAAT CAGTGAGTGA CCAGGATAAT 
TACATTCCCT GTACCCTGGA GCCTGTGTTT GGAAAGATGT TCGAGCTGAC CTGCACTCTG 
CCTCTGGAGA AGGACCTGAA GATCACACTC TACGACTATG ACCTCCTCTC CAAAGATGAG 
AAGATCGGGG AGACAGTCAT CGACCTGGAG AATAGGCTGC TGTCCAAGTT CGGGGCTCGC 
TGCGGACTCC CACAGACCTA CTGTGTCTCT GGACCCAACC AGTGGAGGGA CCAGCTCCGC 
CCCTCCCAGC TTCTCCAGCT CACCTGCCAG CAGCGCAGAC TCAAGGCGCC CGTGTACCTC 
ACAGACCGCG TACTCTTTCA GGATAAAGAA TACACCATTG AGGAAATAGA GGCTGGCAGG 
GTCCTGAACC CACACCTGGG CCCAGTGGAA GAGCGTCTGG CCTTGCACGT CCTCCAGCAG 
CTGGGCCTGG TCCCCGAGCA TGTGGAGTCC CGGCCTCTTT ACAGCCCCCT GCAGCCAGAC 
ATTGAGCAGG GGAAGCTGCA GATGTGGATC GATCTGTTTC CCAAGGCCCT GGGGCGACCT 
GGACCTCCCT TCAACATCAC CCCACGGAGA GCAAGAAGGT TTTTCCTGCG TTGTATTATC 
TGGAACACAA AGGACGTGAT CCTGGACGAC CTCAGCATCA CGGGGGAGAA GATGAGTGAC 
ATTTACGTGA AAGGTTGGAT GGTCGGCTTT GAAGAGCACA AGCAGAAGAC GGACGTGCAC 
TACCGCTCCC TGGGAGGCGA AGGCAACTTT AACTGGAGGT TCATTTTCCC CTTTGACTAC 
CTGCCCGCTG AGCAAGTCTG CACCGTCTCT AAGAAGGATG CTTTCTGGAG GCTGGACAAG 
ACTGAGAGCA AAATCCCAGC ACAAGTGGTA TTTCAGATCT GGGACAATGA CAAGTTCTCC 
TTTGACGACT TTCTGGGCTC GTTGCAGTTG GATCTCAACC GCATGCCCAA GCCTGCCAAG 
ACAGCTGAGA AGTGCTCCTT GGACCAGCTG GACGACACTT TCCACCCGGA GTGGTTTGTG 
TCCCTTTTTG AACAGAAAAC AGTAAAGGGC TGGTGGCCCT GTGTGGCAGA AGACGGTGAG 
AAGAAGATCC TGGCGGGCAA GCTGGAAATG ACCTTGGAGA TCGTAGCAGA GAGTGAGCAC 
GAAGAGCGGC CTGCGGGTCA AGGCCGGGAT GAACCCAACA TGAACCCCAA GCTCGAGGAC 
CCAAGGCGCC CCGACACCTC CTTCCTGTGG TTCACCTCTC CGTACAAGAC CATGAAGTTC 
ATCCTGTGGC GGCGGTTCCG GTGCGCCATC ATCCTCTTCG TCGTTCTCGT CATCCTGCTG 
CTCTTCCTGG GCATCTTCCT CTATTCCTTC CCGAACTATG CTGCCATGAA GCTGGTGAAA 
CCCTTCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_019794831.1 ) cDNA ORF clone, Ailuropoda melanoleuca(giant panda) -> XP_019650390.1 Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X18, mRNA.

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CloneID	OAc89685
Clone ID Related Accession (Same CDS sequence)	XM_019794831.1
Accession Version	XM_019794831.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6252bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-27
Organism	Ailuropoda melanoleuca(giant panda)
Product	dysferlin isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003217356.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ailuropoda melanoleuca Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGTGAAGAA GGACCGGCGC 
AGTGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCAGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTCCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCGG GAGGTCCTCG CCACCCCCAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCCTC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGTGGCTG ACACAGGAGG AGAGGAGGAC 
ACTGAGGACC AGGGGCTCAC GGGAGATGAG GCAGAGCCAT TACTGGATCC GAGCGGTGCC 
CCAGGCCCTG GGGCCCCCAC CACCCCGAGG AAGCCACCTT CCCATCCGCC CCCCTACCAC 
CCCGGGAGCA AGAGAAAAAG AGGTGCTCCC ACGCCCAGAA AGCTGCTGTC GGACAAACCG 
CAGGACTTCC AGATCAGGGT GCAAGTGATC GAGGGGCGCC AGCTGCCAGG GGTGAATATC 
AAGCCTGTGG TCAAAGTCAC GGCTGCCGGG CAGACCAAGA GGACACGGAT CCACAAGGGA 
AACAGCCCAC TCTTCAACGA GACTCTCTTC TTCAATGTGT TTGACTCTCC CTCGGAGCTG 
TTTGACGAGC CCATCTTTAT CACGGTGGTA GACTCCCGTT CTCTCCGGAC AGACGCTCTC 
ATTGGGGAAT TCCGGATGGA CGTGGGTACC ATCTACAGAG AGCCCCGACA CGCCTATCTG 
AGGAAATGGC TTCTCCTCTC GGACCCTGAC GATTTCTCTG CTGGGGCCAG AGGTTACCTG 
AAAGCAAGCC TCTGTGTGCT GGGGCCCGGC GATGAAGCGC CTCTGGAGAG AAAGGACCCC 
TCTGAAGACA AGGAGGACAT CGAAAACAAT CTGCTCAGGC CAACTGGCGT GGCCCTTCGA 
GGAGCCCACT TCTGCCTGAA GGTCTTCAGA GCTGAGGACT TACCACAGAT GGATGATGCC 
GTGATGGACA ACGTGAAGCA GATCTTCGGC TTTGACAGTA ACAAGAAGAA CTTGGTGGAT 
CCCTTCATGG AAGTCAGCTT TGCGGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAAACA 
GCCAACCCTC AGTGGAACCA GAGCATCACG CTGCCCGCCA TGTTTCCCTC CATGTGTGAA 
AAAATGAGGA TTCGCGTCAT GGACTGGGAC CGCCTCACCC ACAATGACAT CGTGGCCACC 
ACCTACCTGA GTATGTCGAA AATCTCCGCC CCTGGAGGAG AAATAGAAGA GGAGCCTGCA 
GGTGTGGCCA AGCCTTCTCC AGCCATGGAC CTGGACGACC ATCTGGGCTT CCTCCCCACC 
TTCGGGCCCT GCTACGTCAA CCTCTACGGC AGCCCCAGGG AGTTCACCGG CTTCCCGGAC 
CCCTACGCAG AACTCAACAT GGGCAAGGGA GAAGGTGTGG CCTACCGAGG TCGGCTTCTG 
CTCTCCCTGG AGACCAAGCT GGTGGAGCAC AGCGACCAAA AGGTGGAGGA CCTCCCTGCG 
GATGACATCC TTCGGGTGGA GAAGTATCTC CGGAGGCGTA AGTACTCCCT CTTTGCCGCC 
TTCTACTCGG CCTCCATGCT CCAGGATGTG GATGATGCCA TCCAGTTCGA GGTCAGCATC 
GGGAACTATG GCAACAAGTT CGACACCACG TGCCTGCCGC TGGCCTCTAC CACCCAGTAC 
AGCCGGGCGG TCTTTGACGG GTGCCACTAC TACTACTTGC CCTGGGGCAA CGTGAAGCCC 
GTGGTGGTGC TGTCATCCTA CTGGGAGGAC ATCAGCCACA GAGTTGAGGC TCAGAACCAG 
CTGCTCAGGA TTGCGGACCG GCTGGAAGCT GCCCTGGAGC AAGTCCACTT GGCCGTGAAG 
GCGCAGTACA ACGCCGAGGA CGTGGACTCC CTGTTGGCTC AGCTGATGGA TGAGCTCATC 
TCAGACTGCA GCCAGCCCCT AGGCGATGTC CACGAGACAC CCTCTGCCAC CCACCTGGAC 
CAGTACCTGC ACAGGCTGCG TAGCCGTCAC CTAAGCCAGA TTGCAGAGGC GGCGCTGGCC 
CTGAAGCTCG GGCACGGCGG GCTCCCGGCC GCCCTGGAGC AGGCTGAAGA CTGGCTCCTG 
CGGCTCCGTG CCCTGGCCGA GGAGCCTCAG AACAGCCTGC CCGACATCAT CATCTGGATG 
CTGCAGGGCG ACAAGCGAGT GGCATACCAG CGGGTGCCTA CACATGAGGT CCTCTTCTCC 
CGGCGTGGAG CCAGCTACTG CGGCAAGAAC TGTGGGAAGC TGCAGACGAT ATTTCTCAAA 
TATCCCATGC AGGGGGTGCC CGGAGCCCGG ATGCCCGTGC AGATACGGGT GAAGCTCTGG 
TTTGGGCTTT CTGTGGATGA GAAGGAGTTC AACCAGTTTG CTGAGGGAAA GCTGTCTGTC 
TTTGCTGAAA CCTATGAGAA CCAGACCAAA CTGGCCCTCG TTGGGAACTG GGGCACAACA 
GGCCTTACCT ACCCCAAGTT TTCTGATGTC GCGGGCAAGA TCAAGCTCCC CAAGGATAGC 
TTCCGCCCCT CAGCCGGCTG GACCTGGGCT GGAGATTGGT TTGTGTGTCC AGAGAAGACG 
CTGCTCCATG ACACCGACGC GGGCCACCTG AGTTTCGTGG CGGAGGTGTT TGAGAACCAG 
ACCCGGCTCC CTGGAGGCCA CTGGGTCTAC ATGAGTGACA ACTACACGGA TGTGAATGGG 
GAGAAGGTGC TTCCCAAGGA TGACATTGAG TGCCCGCTGG GCTGGAAGTG GGAAGATGAA 
GAGTGGTCCA CGGACCTCAA TCGGGCCGTT GATGAGCAAG GCTGGGAGTA CAGTATCACC 
ATCCCCCCGG ACCGGAAGCC GAAGCACTGG GTCCCCGCTG AGAAGATGTA CTACACGCAC 
CGTCGGCGGC GCTGGGTCCG CCTGCGCAGG AGGGACCTCA GCCAGATGGA GGCCCTGAGG 
AAGCACAGGC AGGCGGAGGC GGAGGGCGAG GGCTGGGAGT ACGCCTCGCT CTTTGGCTGG 
AAATTCCACC TGGAGTACCG CAAAACGGAT GCATTCCGTC GCCGCCGCTG GCGCCGCCGC 
ATGGAGCCGC TGGAGAAGAC GGGGCCTGCA GCCGTGTTTG CCCTTGAGGG GGCACTGGGT 
GGCGTGGTAG ATGACCGGAG TGAAGATTCC ATGTCCGTGT CCACCTTGAG CTTTGGCGTG 
AACAGACCCA CGATTTCCTG CATCTTTGAT CATGGGAACC GCTACCACCT GCGCTGCTAT 
ATGTACCAGG CTCGGGACCT GCCCTCGATG GACAAGGACT CTTTTTCTGA TCCCTACGCC 
ATCGTCTCCT TCCTGCACCA GAGCCAGAAG ACCGTGGTGG TGAAGAACAC TCTGAACCCC 
ACCTGGGACC AGACGCTCAT CTTCTATGAG ATTGAGATCT TTGGAGAGCC AGCCAGCATC 
GCCGAGCAGC CGCCCAGCAT TGTGGTGGAG CTGTATGACC ACGACACCTA CGGTGCAGAT 
GAGTTTTTGG GACGCTGCAT GTGTCAGCCC AGTCTGGAAC GGATGCCCCG GCTGGCCTGG 
TTCCCGCTGG TTAGGGGCAG CCAGCCGGCA GGCGAGCTGC TGGCCTCTTT TGAGCTCATC 
CAGAGAGAGA AGCCGGCCAT TCACCATATT CCTGGTTTTG AGGTGCAGGA TACATCGGGA 
ATCCTGGACG AGTCGGAGGA CACAGACCTG CCCTACCCAC CGCCTCAGAG GGAGGCCAAC 
ATCTACATGG TTCCCCAGAA CATCAAGCCG GTTCTCCAGC GCACTGCCAT AGAGATCCTG 
GCATGGGGCC TACGGAACAT GAAGAGTTAC CAGCTGGCCA GTATCTCCTC CCCCAGCCTC 
GTGGTGGAGT GCGGGGGCCA GACGGTGCAG TCCTGTGTCA TCAGGAACCT CCGGAAGAAC 
CCCAACTTCG ACATCTGCAC CCTCTTCATG GAAGTGATGC TGCCCAGAGA GGAGCTCTAC 
TGTCCCCCCA TCGTGATCAA GGTCATTGAT AACCGCCAGT TTGGCCGCCG GCCTGTGGTG 
GGCCAGTGTA CCATACGCTC CCTGGAGAGC TTCATGTGTG ACCTGTACTC CGAGGAGAGT 
CCGTCCCCAC AGGGCGGCCC AGATGATGTG AGTTTACTCA GTCCTGGGGA AGACGTGCTC 
ATTGACATCG ACGACAAGGA GCCCCTCATC CCCATACAGG AGGAGGAGTT CATCGACTGG 
TGGAGCAAAT TCTTTGCCTC CACAGGGGAG AGGGAGAAGT GCGGCTCGTA CTTGGAGAAG 
GGCTTTGACA CTCTAAAGGT CTATGACACA CAACTGGAGA ACGTGGAGGC CTTTGGGGGC 
CTGTCTGACT TTTGTAACAC CTTCAAGCTC TACCGGGGCA AGACTCAGGA GGAGACGGAG 
GACCCATCTG TTATCGGGGA GTTTAAGGGC CTCTTCAAAA TCTACCCCCT CCCGGAAGAC 
CCGGCCGTCC CCATGCCCCC GAGACAGTTC CAGCAGCTGG CTGCCCAGGG GCCTCAGGAC 
TGCCTGGTCC GCATCTATAT CATCCGGGCA TTTGGCCTGC AGCCCAAGGA CCCCAATGGG 
AAGTGTGATC CTTACATCAA GATCTCCATA GGGAAGAAAT CAGTGAGTGA CCAGGATAAT 
TACATTCCCT GTACCCTGGA GCCTGTGTTT GGAAAGATGT TCGAGCTGAC CTGCACTCTG 
CCTCTGGAGA AGGACCTGAA GATCACACTC TACGACTATG ACCTCCTCTC CAAAGATGAG 
AAGATCGGGG AGACAGTCAT CGACCTGGAG AATAGGCTGC TGTCCAAGTT CGGGGCTCGC 
TGCGGACTCC CACAGACCTA CTGTGTCTCT GGACCCAACC AGTGGAGGGA CCAGCTCCGC 
CCCTCCCAGC TTCTCCAGCT CACCTGCCAG CAGCGCAGAC TCAAGGCGCC CGTGTACCTC 
ACAGACCGCG TACTCTTTCA GGATAAAGAA TACACCATTG AGGAAATAGA GGCTGGCAGG 
GTCCTGAACC CACACCTGGG CCCAGTGGAA GAGCGTCTGG CCTTGCACGT CCTCCAGCAG 
CTGGGCCTGG TCCCCGAGCA TGTGGAGTCC CGGCCTCTTT ACAGCCCCCT GCAGCCAGAC 
ATTGAGCAGG GGAAGCTGCA GATGTGGATC GATCTGTTTC CCAAGGCCCT GGGGCGACCT 
GGACCTCCCT TCAACATCAC CCCACGGAGA GCAAGAAGGT TTTTCCTGCG TTGTATTATC 
TGGAACACAA AGGACGTGAT CCTGGACGAC CTCAGCATCA CGGGGGAGAA GATGAGTGAC 
ATTTACGTGA AAGGTTGGAT GGTCGGCTTT GAAGAGCACA AGCAGAAGAC GGACGTGCAC 
TACCGCTCCC TGGGAGGCGA AGGCAACTTT AACTGGAGGT TCATTTTCCC CTTTGACTAC 
CTGCCCGCTG AGCAAGTCTG CACCGTCTCT AAGAAGGATG CTTTCTGGAG GCTGGACAAG 
ACTGAGAGCA AAATCCCAGC ACAAGTGGTA TTTCAGATCT GGGACAATGA CAAGTTCTCC 
TTTGACGACT TTCTGGGCTC GTTGCAGTTG GATCTCAACC GCATGCCCAA GCCTGCCAAG 
ACAGCTGAGA AGTGCTCCTT GGACCAGCTG GACGACACTT TCCACCCGGA GTGGTTTGTG 
TCCCTTTTTG AACAGAAAAC AGTAAAGGGC TGGTGGCCCT GTGTGGCAGA AGACGGTGAG 
AAGAAGATCC TGGCGGGCAA GCTGGAAATG ACCTTGGAGA TCGTAGCAGA GAGTGAGCAC 
GAAGAGCGGC CTGCGGGTCA AGGCCGGGAT GAACCCAACA TGAACCCCAA GCTCGAGGAC 
CCAAGGCGCC CCGACACCTC CTTCCTGTGG TTCACCTCTC CGTACAAGAC CATGAAGTTC 
ATCCTGTGGC GGCGGTTCCG GTGCGCCATC ATCCTCTTCG TCGTTCTCGT CATCCTGCTG 
CTCTTCCTGG GCATCTTCCT CTATTCCTTC CCGAACTATG CTGCCATGAA GCTGGTGAAA 
CCCTTCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019650390.1
CDS	150..6401
Translation	MLSCLLVRASNLPSVKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEAQVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPSASLEPSPTLPDMDMVADTGGEEDTEDQGLTGDEAEPLLDPSGAPGPGAPTTPRKPPSHPPPYHPGSKRKRGAPTPRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVMDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGVAKPSPAMDLDDHLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRLLLSLETKLVEHSDQKVEDLPADDILRVEKYLRRRKYSLFAAFYSASMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADRLEAALEQVHLAVKAQYNAEDVDSLLAQLMDELISDCSQPLGDVHETPSATHLDQYLHRLRSRHLSQIAEAALALKLGHGGLPAALEQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPTHEVLFSRRGASYCGKNCGKLQTIFLKYPMQGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVAGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDTDAGHLSFVAEVFENQTRLPGGHWVYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALRKHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSMSVSTLSFGVNRPTISCIFDHGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFLGRCMCQPSLERMPRLAWFPLVRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTSGILDESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPIVIKVIDNRQFGRRPVVGQCTIRSLESFMCDLYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASTGEREKCGSYLEKGFDTLKVYDTQLENVEAFGGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAVPMPPRQFQQLAAQGPQDCLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLQLTCQQRRLKAPVYLTDRVLFQDKEYTIEEIEAGRVLNPHLGPVEERLALHVLQQLGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPAQVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEDGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFVVLVILLLFLGIFLYSFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_019794831.1
Organism	Ailuropoda melanoleuca(giant panda)
Definition	Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019794831.1

ORF Insert Sequence:

ATGCTGAGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGTGAAGAA GGACCGGCGC 
AGTGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCAGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTCCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCGG GAGGTCCTCG CCACCCCCAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCCTC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGTGGCTG ACACAGGAGG AGAGGAGGAC 
ACTGAGGACC AGGGGCTCAC GGGAGATGAG GCAGAGCCAT TACTGGATCC GAGCGGTGCC 
CCAGGCCCTG GGGCCCCCAC CACCCCGAGG AAGCCACCTT CCCATCCGCC CCCCTACCAC 
CCCGGGAGCA AGAGAAAAAG AGGTGCTCCC ACGCCCAGAA AGCTGCTGTC GGACAAACCG 
CAGGACTTCC AGATCAGGGT GCAAGTGATC GAGGGGCGCC AGCTGCCAGG GGTGAATATC 
AAGCCTGTGG TCAAAGTCAC GGCTGCCGGG CAGACCAAGA GGACACGGAT CCACAAGGGA 
AACAGCCCAC TCTTCAACGA GACTCTCTTC TTCAATGTGT TTGACTCTCC CTCGGAGCTG 
TTTGACGAGC CCATCTTTAT CACGGTGGTA GACTCCCGTT CTCTCCGGAC AGACGCTCTC 
ATTGGGGAAT TCCGGATGGA CGTGGGTACC ATCTACAGAG AGCCCCGACA CGCCTATCTG 
AGGAAATGGC TTCTCCTCTC GGACCCTGAC GATTTCTCTG CTGGGGCCAG AGGTTACCTG 
AAAGCAAGCC TCTGTGTGCT GGGGCCCGGC GATGAAGCGC CTCTGGAGAG AAAGGACCCC 
TCTGAAGACA AGGAGGACAT CGAAAACAAT CTGCTCAGGC CAACTGGCGT GGCCCTTCGA 
GGAGCCCACT TCTGCCTGAA GGTCTTCAGA GCTGAGGACT TACCACAGAT GGATGATGCC 
GTGATGGACA ACGTGAAGCA GATCTTCGGC TTTGACAGTA ACAAGAAGAA CTTGGTGGAT 
CCCTTCATGG AAGTCAGCTT TGCGGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAAACA 
GCCAACCCTC AGTGGAACCA GAGCATCACG CTGCCCGCCA TGTTTCCCTC CATGTGTGAA 
AAAATGAGGA TTCGCGTCAT GGACTGGGAC CGCCTCACCC ACAATGACAT CGTGGCCACC 
ACCTACCTGA GTATGTCGAA AATCTCCGCC CCTGGAGGAG AAATAGAAGA GGAGCCTGCA 
GGTGTGGCCA AGCCTTCTCC AGCCATGGAC CTGGACGACC ATCTGGGCTT CCTCCCCACC 
TTCGGGCCCT GCTACGTCAA CCTCTACGGC AGCCCCAGGG AGTTCACCGG CTTCCCGGAC 
CCCTACGCAG AACTCAACAT GGGCAAGGGA GAAGGTGTGG CCTACCGAGG TCGGCTTCTG 
CTCTCCCTGG AGACCAAGCT GGTGGAGCAC AGCGACCAAA AGGTGGAGGA CCTCCCTGCG 
GATGACATCC TTCGGGTGGA GAAGTATCTC CGGAGGCGTA AGTACTCCCT CTTTGCCGCC 
TTCTACTCGG CCTCCATGCT CCAGGATGTG GATGATGCCA TCCAGTTCGA GGTCAGCATC 
GGGAACTATG GCAACAAGTT CGACACCACG TGCCTGCCGC TGGCCTCTAC CACCCAGTAC 
AGCCGGGCGG TCTTTGACGG GTGCCACTAC TACTACTTGC CCTGGGGCAA CGTGAAGCCC 
GTGGTGGTGC TGTCATCCTA CTGGGAGGAC ATCAGCCACA GAGTTGAGGC TCAGAACCAG 
CTGCTCAGGA TTGCGGACCG GCTGGAAGCT GCCCTGGAGC AAGTCCACTT GGCCGTGAAG 
GCGCAGTACA ACGCCGAGGA CGTGGACTCC CTGTTGGCTC AGCTGATGGA TGAGCTCATC 
TCAGACTGCA GCCAGCCCCT AGGCGATGTC CACGAGACAC CCTCTGCCAC CCACCTGGAC 
CAGTACCTGC ACAGGCTGCG TAGCCGTCAC CTAAGCCAGA TTGCAGAGGC GGCGCTGGCC 
CTGAAGCTCG GGCACGGCGG GCTCCCGGCC GCCCTGGAGC AGGCTGAAGA CTGGCTCCTG 
CGGCTCCGTG CCCTGGCCGA GGAGCCTCAG AACAGCCTGC CCGACATCAT CATCTGGATG 
CTGCAGGGCG ACAAGCGAGT GGCATACCAG CGGGTGCCTA CACATGAGGT CCTCTTCTCC 
CGGCGTGGAG CCAGCTACTG CGGCAAGAAC TGTGGGAAGC TGCAGACGAT ATTTCTCAAA 
TATCCCATGC AGGGGGTGCC CGGAGCCCGG ATGCCCGTGC AGATACGGGT GAAGCTCTGG 
TTTGGGCTTT CTGTGGATGA GAAGGAGTTC AACCAGTTTG CTGAGGGAAA GCTGTCTGTC 
TTTGCTGAAA CCTATGAGAA CCAGACCAAA CTGGCCCTCG TTGGGAACTG GGGCACAACA 
GGCCTTACCT ACCCCAAGTT TTCTGATGTC GCGGGCAAGA TCAAGCTCCC CAAGGATAGC 
TTCCGCCCCT CAGCCGGCTG GACCTGGGCT GGAGATTGGT TTGTGTGTCC AGAGAAGACG 
CTGCTCCATG ACACCGACGC GGGCCACCTG AGTTTCGTGG CGGAGGTGTT TGAGAACCAG 
ACCCGGCTCC CTGGAGGCCA CTGGGTCTAC ATGAGTGACA ACTACACGGA TGTGAATGGG 
GAGAAGGTGC TTCCCAAGGA TGACATTGAG TGCCCGCTGG GCTGGAAGTG GGAAGATGAA 
GAGTGGTCCA CGGACCTCAA TCGGGCCGTT GATGAGCAAG GCTGGGAGTA CAGTATCACC 
ATCCCCCCGG ACCGGAAGCC GAAGCACTGG GTCCCCGCTG AGAAGATGTA CTACACGCAC 
CGTCGGCGGC GCTGGGTCCG CCTGCGCAGG AGGGACCTCA GCCAGATGGA GGCCCTGAGG 
AAGCACAGGC AGGCGGAGGC GGAGGGCGAG GGCTGGGAGT ACGCCTCGCT CTTTGGCTGG 
AAATTCCACC TGGAGTACCG CAAAACGGAT GCATTCCGTC GCCGCCGCTG GCGCCGCCGC 
ATGGAGCCGC TGGAGAAGAC GGGGCCTGCA GCCGTGTTTG CCCTTGAGGG GGCACTGGGT 
GGCGTGGTAG ATGACCGGAG TGAAGATTCC ATGTCCGTGT CCACCTTGAG CTTTGGCGTG 
AACAGACCCA CGATTTCCTG CATCTTTGAT CATGGGAACC GCTACCACCT GCGCTGCTAT 
ATGTACCAGG CTCGGGACCT GCCCTCGATG GACAAGGACT CTTTTTCTGA TCCCTACGCC 
ATCGTCTCCT TCCTGCACCA GAGCCAGAAG ACCGTGGTGG TGAAGAACAC TCTGAACCCC 
ACCTGGGACC AGACGCTCAT CTTCTATGAG ATTGAGATCT TTGGAGAGCC AGCCAGCATC 
GCCGAGCAGC CGCCCAGCAT TGTGGTGGAG CTGTATGACC ACGACACCTA CGGTGCAGAT 
GAGTTTTTGG GACGCTGCAT GTGTCAGCCC AGTCTGGAAC GGATGCCCCG GCTGGCCTGG 
TTCCCGCTGG TTAGGGGCAG CCAGCCGGCA GGCGAGCTGC TGGCCTCTTT TGAGCTCATC 
CAGAGAGAGA AGCCGGCCAT TCACCATATT CCTGGTTTTG AGGTGCAGGA TACATCGGGA 
ATCCTGGACG AGTCGGAGGA CACAGACCTG CCCTACCCAC CGCCTCAGAG GGAGGCCAAC 
ATCTACATGG TTCCCCAGAA CATCAAGCCG GTTCTCCAGC GCACTGCCAT AGAGATCCTG 
GCATGGGGCC TACGGAACAT GAAGAGTTAC CAGCTGGCCA GTATCTCCTC CCCCAGCCTC 
GTGGTGGAGT GCGGGGGCCA GACGGTGCAG TCCTGTGTCA TCAGGAACCT CCGGAAGAAC 
CCCAACTTCG ACATCTGCAC CCTCTTCATG GAAGTGATGC TGCCCAGAGA GGAGCTCTAC 
TGTCCCCCCA TCGTGATCAA GGTCATTGAT AACCGCCAGT TTGGCCGCCG GCCTGTGGTG 
GGCCAGTGTA CCATACGCTC CCTGGAGAGC TTCATGTGTG ACCTGTACTC CGAGGAGAGT 
CCGTCCCCAC AGGGCGGCCC AGATGATGTG AGTTTACTCA GTCCTGGGGA AGACGTGCTC 
ATTGACATCG ACGACAAGGA GCCCCTCATC CCCATACAGG AGGAGGAGTT CATCGACTGG 
TGGAGCAAAT TCTTTGCCTC CACAGGGGAG AGGGAGAAGT GCGGCTCGTA CTTGGAGAAG 
GGCTTTGACA CTCTAAAGGT CTATGACACA CAACTGGAGA ACGTGGAGGC CTTTGGGGGC 
CTGTCTGACT TTTGTAACAC CTTCAAGCTC TACCGGGGCA AGACTCAGGA GGAGACGGAG 
GACCCATCTG TTATCGGGGA GTTTAAGGGC CTCTTCAAAA TCTACCCCCT CCCGGAAGAC 
CCGGCCGTCC CCATGCCCCC GAGACAGTTC CAGCAGCTGG CTGCCCAGGG GCCTCAGGAC 
TGCCTGGTCC GCATCTATAT CATCCGGGCA TTTGGCCTGC AGCCCAAGGA CCCCAATGGG 
AAGTGTGATC CTTACATCAA GATCTCCATA GGGAAGAAAT CAGTGAGTGA CCAGGATAAT 
TACATTCCCT GTACCCTGGA GCCTGTGTTT GGAAAGATGT TCGAGCTGAC CTGCACTCTG 
CCTCTGGAGA AGGACCTGAA GATCACACTC TACGACTATG ACCTCCTCTC CAAAGATGAG 
AAGATCGGGG AGACAGTCAT CGACCTGGAG AATAGGCTGC TGTCCAAGTT CGGGGCTCGC 
TGCGGACTCC CACAGACCTA CTGTGTCTCT GGACCCAACC AGTGGAGGGA CCAGCTCCGC 
CCCTCCCAGC TTCTCCAGCT CACCTGCCAG CAGCGCAGAC TCAAGGCGCC CGTGTACCTC 
ACAGACCGCG TACTCTTTCA GGATAAAGAA TACACCATTG AGGAAATAGA GGCTGGCAGG 
GTCCTGAACC CACACCTGGG CCCAGTGGAA GAGCGTCTGG CCTTGCACGT CCTCCAGCAG 
CTGGGCCTGG TCCCCGAGCA TGTGGAGTCC CGGCCTCTTT ACAGCCCCCT GCAGCCAGAC 
ATTGAGCAGG GGAAGCTGCA GATGTGGATC GATCTGTTTC CCAAGGCCCT GGGGCGACCT 
GGACCTCCCT TCAACATCAC CCCACGGAGA GCAAGAAGGT TTTTCCTGCG TTGTATTATC 
TGGAACACAA AGGACGTGAT CCTGGACGAC CTCAGCATCA CGGGGGAGAA GATGAGTGAC 
ATTTACGTGA AAGGTTGGAT GGTCGGCTTT GAAGAGCACA AGCAGAAGAC GGACGTGCAC 
TACCGCTCCC TGGGAGGCGA AGGCAACTTT AACTGGAGGT TCATTTTCCC CTTTGACTAC 
CTGCCCGCTG AGCAAGTCTG CACCGTCTCT AAGAAGGATG CTTTCTGGAG GCTGGACAAG 
ACTGAGAGCA AAATCCCAGC ACAAGTGGTA TTTCAGATCT GGGACAATGA CAAGTTCTCC 
TTTGACGACT TTCTGGGCTC GTTGCAGTTG GATCTCAACC GCATGCCCAA GCCTGCCAAG 
ACAGCTGAGA AGTGCTCCTT GGACCAGCTG GACGACACTT TCCACCCGGA GTGGTTTGTG 
TCCCTTTTTG AACAGAAAAC AGTAAAGGGC TGGTGGCCCT GTGTGGCAGA AGACGGTGAG 
AAGAAGATCC TGGCGGGCAA GCTGGAAATG ACCTTGGAGA TCGTAGCAGA GAGTGAGCAC 
GAAGAGCGGC CTGCGGGTCA AGGCCGGGAT GAACCCAACA TGAACCCCAA GCTCGAGGAC 
CCAAGGCGCC CCGACACCTC CTTCCTGTGG TTCACCTCTC CGTACAAGAC CATGAAGTTC 
ATCCTGTGGC GGCGGTTCCG GTGCGCCATC ATCCTCTTCG TCGTTCTCGT CATCCTGCTG 
CTCTTCCTGG GCATCTTCCT CTATTCCTTC CCGAACTATG CTGCCATGAA GCTGGTGAAA 
CCCTTCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_019794832.1 ) cDNA ORF clone, Ailuropoda melanoleuca(giant panda) -> XP_019650391.1 Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X19, mRNA.

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CloneID	OAc89686
Clone ID Related Accession (Same CDS sequence)	XM_019794832.1
Accession Version	XM_019794832.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6249bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-27
Organism	Ailuropoda melanoleuca(giant panda)
Product	dysferlin isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003217356.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ailuropoda melanoleuca Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTATGCCGAG AATGTCCACA CCCCGGACAC CGACATCAGC 
GATGCCTACT GCTCTGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CAGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGAGCTCCG AGCTCCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
TTAGGGGAAG CCCAAGTCCC ACTCCGGGAG GTCCTCGCCA CCCCCAGTCT GTCTGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CGCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCCTCTGC ATCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATATG GTGGCTGACA CAGGAGGAGA GGAGGACACT 
GAGGACCAGG GGCTCACGGG AGATGAGGCA GAGCCATTAC TGGATCCGAG CGGTGCCCCA 
GGCCCTGGGG CCCCCACCAC CCCGAGGAAG CCACCTTCCC ATCCGCCCCC CTACCACCCC 
GGGAGCAAGA GAAAAAGAGG TGCTCCCACG CCCAGAAAGC TGCTGTCGGA CAAACCGCAG 
GACTTCCAGA TCAGGGTGCA AGTGATCGAG GGGCGCCAGC TGCCAGGGGT GAATATCAAG 
CCTGTGGTCA AAGTCACGGC TGCCGGGCAG ACCAAGAGGA CACGGATCCA CAAGGGAAAC 
AGCCCACTCT TCAACGAGAC TCTCTTCTTC AATGTGTTTG ACTCTCCCTC GGAGCTGTTT 
GACGAGCCCA TCTTTATCAC GGTGGTAGAC TCCCGTTCTC TCCGGACAGA CGCTCTCATT 
GGGGAATTCC GGATGGACGT GGGTACCATC TACAGAGAGC CCCGACACGC CTATCTGAGG 
AAATGGCTTC TCCTCTCGGA CCCTGACGAT TTCTCTGCTG GGGCCAGAGG TTACCTGAAA 
GCAAGCCTCT GTGTGCTGGG GCCCGGCGAT GAAGCGCCTC TGGAGAGAAA GGACCCCTCT 
GAAGACAAGG AGGACATCGA AAACAATCTG CTCAGGCCAA CTGGCGTGGC CCTTCGAGGA 
GCCCACTTCT GCCTGAAGGT CTTCAGAGCT GAGGACTTAC CACAGATGGA TGATGCCGTG 
ATGGACAACG TGAAGCAGAT CTTCGGCTTT GACAGTAACA AGAAGAACTT GGTGGATCCC 
TTCATGGAAG TCAGCTTTGC GGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAAACAGCC 
AACCCTCAGT GGAACCAGAG CATCACGCTG CCCGCCATGT TTCCCTCCAT GTGTGAAAAA 
ATGAGGATTC GCGTCATGGA CTGGGACCGC CTCACCCACA ATGACATCGT GGCCACCACC 
TACCTGAGTA TGTCGAAAAT CTCCGCCCCT GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT 
GTGGCCAAGC CTTCTCCAGC CATGGACCTG GACGACCATC TGGGCTTCCT CCCCACCTTC 
GGGCCCTGCT ACGTCAACCT CTACGGCAGC CCCAGGGAGT TCACCGGCTT CCCGGACCCC 
TACGCAGAAC TCAACATGGG CAAGGGAGAA GGTGTGGCCT ACCGAGGTCG GCTTCTGCTC 
TCCCTGGAGA CCAAGCTGGT GGAGCACAGC GACCAAAAGG TGGAGGACCT CCCTGCGGAT 
GACATCCTTC GGGTGGAGAA GTATCTCCGG AGGCGTAAGT ACTCCCTCTT TGCCGCCTTC 
TACTCGGCCT CCATGCTCCA GGATGTGGAT GATGCCATCC AGTTCGAGGT CAGCATCGGG 
AACTATGGCA ACAAGTTCGA CACCACGTGC CTGCCGCTGG CCTCTACCAC CCAGTACAGC 
CGGGCGGTCT TTGACGGGTG CCACTACTAC TACTTGCCCT GGGGCAACGT GAAGCCCGTG 
GTGGTGCTGT CATCCTACTG GGAGGACATC AGCCACAGAG TTGAGGCTCA GAACCAGCTG 
CTCAGGATTG CGGACCGGCT GGAAGCTGCC CTGGAGCAAG TCCACTTGGC CGTGAAGGCG 
CAGTACAACG CCGAGGACGT GGACTCCCTG TTGGCTCAGC TGATGGATGA GCTCATCTCA 
GACTGCAGCC AGCCCCTAGG CGATGTCCAC GAGACACCCT CTGCCACCCA CCTGGACCAG 
TACCTGCACA GGCTGCGTAG CCGTCACCTA AGCCAGATTG CAGAGGCGGC GCTGGCCCTG 
AAGCTCGGGC ACGGCGGGCT CCCGGCCGCC CTGGAGCAGG CTGAAGACTG GCTCCTGCGG 
CTCCGTGCCC TGGCCGAGGA GCCTCAGAAC AGCCTGCCCG ACATCATCAT CTGGATGCTG 
CAGGGCGACA AGCGAGTGGC ATACCAGCGG GTGCCTACAC ATGAGGTCCT CTTCTCCCGG 
CGTGGAGCCA GCTACTGCGG CAAGAACTGT GGGAAGCTGC AGACGATATT TCTCAAATAT 
CCCATGCAGG GGGTGCCCGG AGCCCGGATG CCCGTGCAGA TACGGGTGAA GCTCTGGTTT 
GGGCTTTCTG TGGATGAGAA GGAGTTCAAC CAGTTTGCTG AGGGAAAGCT GTCTGTCTTT 
GCTGAAACCT ATGAGAACCA GACCAAACTG GCCCTCGTTG GGAACTGGGG CACAACAGGC 
CTTACCTACC CCAAGTTTTC TGATGTCGCG GGCAAGATCA AGCTCCCCAA GGATAGCTTC 
CGCCCCTCAG CCGGCTGGAC CTGGGCTGGA GATTGGTTTG TGTGTCCAGA GAAGACGCTG 
CTCCATGACA CCGACGCGGG CCACCTGAGT TTCGTGGCGG AGGTGTTTGA GAACCAGACC 
CGGCTCCCTG GAGGCCACTG GGTCTACATG AGTGACAACT ACACGGATGT GAATGGGGAG 
AAGGTGCTTC CCAAGGATGA CATTGAGTGC CCGCTGGGCT GGAAGTGGGA AGATGAAGAG 
TGGTCCACGG ACCTCAATCG GGCCGTTGAT GAGCAAGGCT GGGAGTACAG TATCACCATC 
CCCCCGGACC GGAAGCCGAA GCACTGGGTC CCCGCTGAGA AGATGTACTA CACGCACCGT 
CGGCGGCGCT GGGTCCGCCT GCGCAGGAGG GACCTCAGCC AGATGGAGGC CCTGAGGAAG 
CACAGGCAGG CGGAGGCGGA GGGCGAGGGC TGGGAGTACG CCTCGCTCTT TGGCTGGAAA 
TTCCACCTGG AGTACCGCAA AACGGATGCA TTCCGTCGCC GCCGCTGGCG CCGCCGCATG 
GAGCCGCTGG AGAAGACGGG GCCTGCAGCC GTGTTTGCCC TTGAGGGGGC ACTGGGTGGC 
GTGGTAGATG ACCGGAGTGA AGATTCCATG TCCGTGTCCA CCTTGAGCTT TGGCGTGAAC 
AGACCCACGA TTTCCTGCAT CTTTGATCAT GGGAACCGCT ACCACCTGCG CTGCTATATG 
TACCAGGCTC GGGACCTGCC CTCGATGGAC AAGGACTCTT TTTCTGATCC CTACGCCATC 
GTCTCCTTCC TGCACCAGAG CCAGAAGACC GTGGTGGTGA AGAACACTCT GAACCCCACC 
TGGGACCAGA CGCTCATCTT CTATGAGATT GAGATCTTTG GAGAGCCAGC CAGCATCGCC 
GAGCAGCCGC CCAGCATTGT GGTGGAGCTG TATGACCACG ACACCTACGG TGCAGATGAG 
TTTTTGGGAC GCTGCATGTG TCAGCCCAGT CTGGAACGGA TGCCCCGGCT GGCCTGGTTC 
CCGCTGGTTA GGGGCAGCCA GCCGGCAGGC GAGCTGCTGG CCTCTTTTGA GCTCATCCAG 
AGAGAGAAGC CGGCCATTCA CCATATTCCT GGTTTTGAGG TGCAGGATAC ATCGGGAATC 
CTGGACGAGT CGGAGGACAC AGACCTGCCC TACCCACCGC CTCAGAGGGA GGCCAACATC 
TACATGGTTC CCCAGAACAT CAAGCCGGTT CTCCAGCGCA CTGCCATAGA GATCCTGGCA 
TGGGGCCTAC GGAACATGAA GAGTTACCAG CTGGCCAGTA TCTCCTCCCC CAGCCTCGTG 
GTGGAGTGCG GGGGCCAGAC GGTGCAGTCC TGTGTCATCA GGAACCTCCG GAAGAACCCC 
AACTTCGACA TCTGCACCCT CTTCATGGAA GTGATGCTGC CCAGAGAGGA GCTCTACTGT 
CCCCCCATCG TGATCAAGGT CATTGATAAC CGCCAGTTTG GCCGCCGGCC TGTGGTGGGC 
CAGTGTACCA TACGCTCCCT GGAGAGCTTC ATGTGTGACC TGTACTCCGA GGAGAGTCCG 
TCCCCACAGG GCGGCCCAGA TGATGTGAGT TTACTCAGTC CTGGGGAAGA CGTGCTCATT 
GACATCGACG ACAAGGAGCC CCTCATCCCC ATACAGGAGG AGGAGTTCAT CGACTGGTGG 
AGCAAATTCT TTGCCTCCAC AGGGGAGAGG GAGAAGTGCG GCTCGTACTT GGAGAAGGGC 
TTTGACACTC TAAAGGTCTA TGACACACAA CTGGAGAACG TGGAGGCCTT TGGGGGCCTG 
TCTGACTTTT GTAACACCTT CAAGCTCTAC CGGGGCAAGA CTCAGGAGGA GACGGAGGAC 
CCATCTGTTA TCGGGGAGTT TAAGGGCCTC TTCAAAATCT ACCCCCTCCC GGAAGACCCG 
GCCGTCCCCA TGCCCCCGAG ACAGTTCCAG CAGCTGGCTG CCCAGGGGCC TCAGGACTGC 
CTGGTCCGCA TCTATATCAT CCGGGCATTT GGCCTGCAGC CCAAGGACCC CAATGGGAAG 
TGTGATCCTT ACATCAAGAT CTCCATAGGG AAGAAATCAG TGAGTGACCA GGATAATTAC 
ATTCCCTGTA CCCTGGAGCC TGTGTTTGGA AAGATGTTCG AGCTGACCTG CACTCTGCCT 
CTGGAGAAGG ACCTGAAGAT CACACTCTAC GACTATGACC TCCTCTCCAA AGATGAGAAG 
ATCGGGGAGA CAGTCATCGA CCTGGAGAAT AGGCTGCTGT CCAAGTTCGG GGCTCGCTGC 
GGACTCCCAC AGACCTACTG TGTCTCTGGA CCCAACCAGT GGAGGGACCA GCTCCGCCCC 
TCCCAGCTTC TCCAGCTCAC CTGCCAGCAG CGCAGACTCA AGGCGCCCGT GTACCTCACA 
GACCGCGTAC TCTTTCAGGA TAAAGAATAC ACCATTGAGG AAATAGAGGC TGGCAGGGTC 
CTGAACCCAC ACCTGGGCCC AGTGGAAGAG CGTCTGGCCT TGCACGTCCT CCAGCAGCTG 
GGCCTGGTCC CCGAGCATGT GGAGTCCCGG CCTCTTTACA GCCCCCTGCA GCCAGACATT 
GAGCAGGGGA AGCTGCAGAT GTGGATCGAT CTGTTTCCCA AGGCCCTGGG GCGACCTGGA 
CCTCCCTTCA ACATCACCCC ACGGAGAGCA AGAAGGTTTT TCCTGCGTTG TATTATCTGG 
AACACAAAGG ACGTGATCCT GGACGACCTC AGCATCACGG GGGAGAAGAT GAGTGACATT 
TACGTGAAAG GTTGGATGGT CGGCTTTGAA GAGCACAAGC AGAAGACGGA CGTGCACTAC 
CGCTCCCTGG GAGGCGAAGG CAACTTTAAC TGGAGGTTCA TTTTCCCCTT TGACTACCTG 
CCCGCTGAGC AAGTCTGCAC CGTCTCTAAG AAGGATGCTT TCTGGAGGCT GGACAAGACT 
GAGAGCAAAA TCCCAGCACA AGTGGTATTT CAGATCTGGG ACAATGACAA GTTCTCCTTT 
GACGACTTTC TGGGCTCGTT GCAGTTGGAT CTCAACCGCA TGCCCAAGCC TGCCAAGACA 
GCTGAGAAGT GCTCCTTGGA CCAGCTGGAC GACACTTTCC ACCCGGAGTG GTTTGTGTCC 
CTTTTTGAAC AGAAAACAGT AAAGGGCTGG TGGCCCTGTG TGGCAGAAGA CGGTGAGAAG 
AAGATCCTGG CGGGCAAGCT GGAAATGACC TTGGAGATCG TAGCAGAGAG TGAGCACGAA 
GAGCGGCCTG CGGGTCAAGG CCGGGATGAA CCCAACATGA ACCCCAAGCT CGAGGACCCA 
AGGCGCCCCG ACACCTCCTT CCTGTGGTTC ACCTCTCCGT ACAAGACCAT GAAGTTCATC 
CTGTGGCGGC GGTTCCGGTG CGCCATCATC CTCTTCGTCG TTCTCGTCAT CCTGCTGCTC 
TTCCTGGGCA TCTTCCTCTA TTCCTTCCCG AACTATGCTG CCATGAAGCT GGTGAAACCC 
TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019650391.1
CDS	487..6735
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEAQVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPSASLEPSPTLPDMDMVADTGGEEDTEDQGLTGDEAEPLLDPSGAPGPGAPTTPRKPPSHPPPYHPGSKRKRGAPTPRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVMDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGVAKPSPAMDLDDHLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRLLLSLETKLVEHSDQKVEDLPADDILRVEKYLRRRKYSLFAAFYSASMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADRLEAALEQVHLAVKAQYNAEDVDSLLAQLMDELISDCSQPLGDVHETPSATHLDQYLHRLRSRHLSQIAEAALALKLGHGGLPAALEQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPTHEVLFSRRGASYCGKNCGKLQTIFLKYPMQGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVAGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDTDAGHLSFVAEVFENQTRLPGGHWVYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALRKHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSMSVSTLSFGVNRPTISCIFDHGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFLGRCMCQPSLERMPRLAWFPLVRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTSGILDESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPIVIKVIDNRQFGRRPVVGQCTIRSLESFMCDLYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASTGEREKCGSYLEKGFDTLKVYDTQLENVEAFGGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAVPMPPRQFQQLAAQGPQDCLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLQLTCQQRRLKAPVYLTDRVLFQDKEYTIEEIEAGRVLNPHLGPVEERLALHVLQQLGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPAQVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEDGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFVVLVILLLFLGIFLYSFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_019794832.1
Organism	Ailuropoda melanoleuca(giant panda)
Definition	Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019794832.1

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCCGAG AATGTCCACA CCCCGGACAC CGACATCAGC 
GATGCCTACT GCTCTGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CAGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGAGCTCCG AGCTCCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
TTAGGGGAAG CCCAAGTCCC ACTCCGGGAG GTCCTCGCCA CCCCCAGTCT GTCTGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CGCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCCTCTGC ATCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATATG GTGGCTGACA CAGGAGGAGA GGAGGACACT 
GAGGACCAGG GGCTCACGGG AGATGAGGCA GAGCCATTAC TGGATCCGAG CGGTGCCCCA 
GGCCCTGGGG CCCCCACCAC CCCGAGGAAG CCACCTTCCC ATCCGCCCCC CTACCACCCC 
GGGAGCAAGA GAAAAAGAGG TGCTCCCACG CCCAGAAAGC TGCTGTCGGA CAAACCGCAG 
GACTTCCAGA TCAGGGTGCA AGTGATCGAG GGGCGCCAGC TGCCAGGGGT GAATATCAAG 
CCTGTGGTCA AAGTCACGGC TGCCGGGCAG ACCAAGAGGA CACGGATCCA CAAGGGAAAC 
AGCCCACTCT TCAACGAGAC TCTCTTCTTC AATGTGTTTG ACTCTCCCTC GGAGCTGTTT 
GACGAGCCCA TCTTTATCAC GGTGGTAGAC TCCCGTTCTC TCCGGACAGA CGCTCTCATT 
GGGGAATTCC GGATGGACGT GGGTACCATC TACAGAGAGC CCCGACACGC CTATCTGAGG 
AAATGGCTTC TCCTCTCGGA CCCTGACGAT TTCTCTGCTG GGGCCAGAGG TTACCTGAAA 
GCAAGCCTCT GTGTGCTGGG GCCCGGCGAT GAAGCGCCTC TGGAGAGAAA GGACCCCTCT 
GAAGACAAGG AGGACATCGA AAACAATCTG CTCAGGCCAA CTGGCGTGGC CCTTCGAGGA 
GCCCACTTCT GCCTGAAGGT CTTCAGAGCT GAGGACTTAC CACAGATGGA TGATGCCGTG 
ATGGACAACG TGAAGCAGAT CTTCGGCTTT GACAGTAACA AGAAGAACTT GGTGGATCCC 
TTCATGGAAG TCAGCTTTGC GGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAAACAGCC 
AACCCTCAGT GGAACCAGAG CATCACGCTG CCCGCCATGT TTCCCTCCAT GTGTGAAAAA 
ATGAGGATTC GCGTCATGGA CTGGGACCGC CTCACCCACA ATGACATCGT GGCCACCACC 
TACCTGAGTA TGTCGAAAAT CTCCGCCCCT GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT 
GTGGCCAAGC CTTCTCCAGC CATGGACCTG GACGACCATC TGGGCTTCCT CCCCACCTTC 
GGGCCCTGCT ACGTCAACCT CTACGGCAGC CCCAGGGAGT TCACCGGCTT CCCGGACCCC 
TACGCAGAAC TCAACATGGG CAAGGGAGAA GGTGTGGCCT ACCGAGGTCG GCTTCTGCTC 
TCCCTGGAGA CCAAGCTGGT GGAGCACAGC GACCAAAAGG TGGAGGACCT CCCTGCGGAT 
GACATCCTTC GGGTGGAGAA GTATCTCCGG AGGCGTAAGT ACTCCCTCTT TGCCGCCTTC 
TACTCGGCCT CCATGCTCCA GGATGTGGAT GATGCCATCC AGTTCGAGGT CAGCATCGGG 
AACTATGGCA ACAAGTTCGA CACCACGTGC CTGCCGCTGG CCTCTACCAC CCAGTACAGC 
CGGGCGGTCT TTGACGGGTG CCACTACTAC TACTTGCCCT GGGGCAACGT GAAGCCCGTG 
GTGGTGCTGT CATCCTACTG GGAGGACATC AGCCACAGAG TTGAGGCTCA GAACCAGCTG 
CTCAGGATTG CGGACCGGCT GGAAGCTGCC CTGGAGCAAG TCCACTTGGC CGTGAAGGCG 
CAGTACAACG CCGAGGACGT GGACTCCCTG TTGGCTCAGC TGATGGATGA GCTCATCTCA 
GACTGCAGCC AGCCCCTAGG CGATGTCCAC GAGACACCCT CTGCCACCCA CCTGGACCAG 
TACCTGCACA GGCTGCGTAG CCGTCACCTA AGCCAGATTG CAGAGGCGGC GCTGGCCCTG 
AAGCTCGGGC ACGGCGGGCT CCCGGCCGCC CTGGAGCAGG CTGAAGACTG GCTCCTGCGG 
CTCCGTGCCC TGGCCGAGGA GCCTCAGAAC AGCCTGCCCG ACATCATCAT CTGGATGCTG 
CAGGGCGACA AGCGAGTGGC ATACCAGCGG GTGCCTACAC ATGAGGTCCT CTTCTCCCGG 
CGTGGAGCCA GCTACTGCGG CAAGAACTGT GGGAAGCTGC AGACGATATT TCTCAAATAT 
CCCATGCAGG GGGTGCCCGG AGCCCGGATG CCCGTGCAGA TACGGGTGAA GCTCTGGTTT 
GGGCTTTCTG TGGATGAGAA GGAGTTCAAC CAGTTTGCTG AGGGAAAGCT GTCTGTCTTT 
GCTGAAACCT ATGAGAACCA GACCAAACTG GCCCTCGTTG GGAACTGGGG CACAACAGGC 
CTTACCTACC CCAAGTTTTC TGATGTCGCG GGCAAGATCA AGCTCCCCAA GGATAGCTTC 
CGCCCCTCAG CCGGCTGGAC CTGGGCTGGA GATTGGTTTG TGTGTCCAGA GAAGACGCTG 
CTCCATGACA CCGACGCGGG CCACCTGAGT TTCGTGGCGG AGGTGTTTGA GAACCAGACC 
CGGCTCCCTG GAGGCCACTG GGTCTACATG AGTGACAACT ACACGGATGT GAATGGGGAG 
AAGGTGCTTC CCAAGGATGA CATTGAGTGC CCGCTGGGCT GGAAGTGGGA AGATGAAGAG 
TGGTCCACGG ACCTCAATCG GGCCGTTGAT GAGCAAGGCT GGGAGTACAG TATCACCATC 
CCCCCGGACC GGAAGCCGAA GCACTGGGTC CCCGCTGAGA AGATGTACTA CACGCACCGT 
CGGCGGCGCT GGGTCCGCCT GCGCAGGAGG GACCTCAGCC AGATGGAGGC CCTGAGGAAG 
CACAGGCAGG CGGAGGCGGA GGGCGAGGGC TGGGAGTACG CCTCGCTCTT TGGCTGGAAA 
TTCCACCTGG AGTACCGCAA AACGGATGCA TTCCGTCGCC GCCGCTGGCG CCGCCGCATG 
GAGCCGCTGG AGAAGACGGG GCCTGCAGCC GTGTTTGCCC TTGAGGGGGC ACTGGGTGGC 
GTGGTAGATG ACCGGAGTGA AGATTCCATG TCCGTGTCCA CCTTGAGCTT TGGCGTGAAC 
AGACCCACGA TTTCCTGCAT CTTTGATCAT GGGAACCGCT ACCACCTGCG CTGCTATATG 
TACCAGGCTC GGGACCTGCC CTCGATGGAC AAGGACTCTT TTTCTGATCC CTACGCCATC 
GTCTCCTTCC TGCACCAGAG CCAGAAGACC GTGGTGGTGA AGAACACTCT GAACCCCACC 
TGGGACCAGA CGCTCATCTT CTATGAGATT GAGATCTTTG GAGAGCCAGC CAGCATCGCC 
GAGCAGCCGC CCAGCATTGT GGTGGAGCTG TATGACCACG ACACCTACGG TGCAGATGAG 
TTTTTGGGAC GCTGCATGTG TCAGCCCAGT CTGGAACGGA TGCCCCGGCT GGCCTGGTTC 
CCGCTGGTTA GGGGCAGCCA GCCGGCAGGC GAGCTGCTGG CCTCTTTTGA GCTCATCCAG 
AGAGAGAAGC CGGCCATTCA CCATATTCCT GGTTTTGAGG TGCAGGATAC ATCGGGAATC 
CTGGACGAGT CGGAGGACAC AGACCTGCCC TACCCACCGC CTCAGAGGGA GGCCAACATC 
TACATGGTTC CCCAGAACAT CAAGCCGGTT CTCCAGCGCA CTGCCATAGA GATCCTGGCA 
TGGGGCCTAC GGAACATGAA GAGTTACCAG CTGGCCAGTA TCTCCTCCCC CAGCCTCGTG 
GTGGAGTGCG GGGGCCAGAC GGTGCAGTCC TGTGTCATCA GGAACCTCCG GAAGAACCCC 
AACTTCGACA TCTGCACCCT CTTCATGGAA GTGATGCTGC CCAGAGAGGA GCTCTACTGT 
CCCCCCATCG TGATCAAGGT CATTGATAAC CGCCAGTTTG GCCGCCGGCC TGTGGTGGGC 
CAGTGTACCA TACGCTCCCT GGAGAGCTTC ATGTGTGACC TGTACTCCGA GGAGAGTCCG 
TCCCCACAGG GCGGCCCAGA TGATGTGAGT TTACTCAGTC CTGGGGAAGA CGTGCTCATT 
GACATCGACG ACAAGGAGCC CCTCATCCCC ATACAGGAGG AGGAGTTCAT CGACTGGTGG 
AGCAAATTCT TTGCCTCCAC AGGGGAGAGG GAGAAGTGCG GCTCGTACTT GGAGAAGGGC 
TTTGACACTC TAAAGGTCTA TGACACACAA CTGGAGAACG TGGAGGCCTT TGGGGGCCTG 
TCTGACTTTT GTAACACCTT CAAGCTCTAC CGGGGCAAGA CTCAGGAGGA GACGGAGGAC 
CCATCTGTTA TCGGGGAGTT TAAGGGCCTC TTCAAAATCT ACCCCCTCCC GGAAGACCCG 
GCCGTCCCCA TGCCCCCGAG ACAGTTCCAG CAGCTGGCTG CCCAGGGGCC TCAGGACTGC 
CTGGTCCGCA TCTATATCAT CCGGGCATTT GGCCTGCAGC CCAAGGACCC CAATGGGAAG 
TGTGATCCTT ACATCAAGAT CTCCATAGGG AAGAAATCAG TGAGTGACCA GGATAATTAC 
ATTCCCTGTA CCCTGGAGCC TGTGTTTGGA AAGATGTTCG AGCTGACCTG CACTCTGCCT 
CTGGAGAAGG ACCTGAAGAT CACACTCTAC GACTATGACC TCCTCTCCAA AGATGAGAAG 
ATCGGGGAGA CAGTCATCGA CCTGGAGAAT AGGCTGCTGT CCAAGTTCGG GGCTCGCTGC 
GGACTCCCAC AGACCTACTG TGTCTCTGGA CCCAACCAGT GGAGGGACCA GCTCCGCCCC 
TCCCAGCTTC TCCAGCTCAC CTGCCAGCAG CGCAGACTCA AGGCGCCCGT GTACCTCACA 
GACCGCGTAC TCTTTCAGGA TAAAGAATAC ACCATTGAGG AAATAGAGGC TGGCAGGGTC 
CTGAACCCAC ACCTGGGCCC AGTGGAAGAG CGTCTGGCCT TGCACGTCCT CCAGCAGCTG 
GGCCTGGTCC CCGAGCATGT GGAGTCCCGG CCTCTTTACA GCCCCCTGCA GCCAGACATT 
GAGCAGGGGA AGCTGCAGAT GTGGATCGAT CTGTTTCCCA AGGCCCTGGG GCGACCTGGA 
CCTCCCTTCA ACATCACCCC ACGGAGAGCA AGAAGGTTTT TCCTGCGTTG TATTATCTGG 
AACACAAAGG ACGTGATCCT GGACGACCTC AGCATCACGG GGGAGAAGAT GAGTGACATT 
TACGTGAAAG GTTGGATGGT CGGCTTTGAA GAGCACAAGC AGAAGACGGA CGTGCACTAC 
CGCTCCCTGG GAGGCGAAGG CAACTTTAAC TGGAGGTTCA TTTTCCCCTT TGACTACCTG 
CCCGCTGAGC AAGTCTGCAC CGTCTCTAAG AAGGATGCTT TCTGGAGGCT GGACAAGACT 
GAGAGCAAAA TCCCAGCACA AGTGGTATTT CAGATCTGGG ACAATGACAA GTTCTCCTTT 
GACGACTTTC TGGGCTCGTT GCAGTTGGAT CTCAACCGCA TGCCCAAGCC TGCCAAGACA 
GCTGAGAAGT GCTCCTTGGA CCAGCTGGAC GACACTTTCC ACCCGGAGTG GTTTGTGTCC 
CTTTTTGAAC AGAAAACAGT AAAGGGCTGG TGGCCCTGTG TGGCAGAAGA CGGTGAGAAG 
AAGATCCTGG CGGGCAAGCT GGAAATGACC TTGGAGATCG TAGCAGAGAG TGAGCACGAA 
GAGCGGCCTG CGGGTCAAGG CCGGGATGAA CCCAACATGA ACCCCAAGCT CGAGGACCCA 
AGGCGCCCCG ACACCTCCTT CCTGTGGTTC ACCTCTCCGT ACAAGACCAT GAAGTTCATC 
CTGTGGCGGC GGTTCCGGTG CGCCATCATC CTCTTCGTCG TTCTCGTCAT CCTGCTGCTC 
TTCCTGGGCA TCTTCCTCTA TTCCTTCCCG AACTATGCTG CCATGAAGCT GGTGAAACCC 
TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_002914883.3 ) cDNA ORF clone, Ailuropoda melanoleuca(giant panda) -> XP_002914929.1 Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X7, mRNA.

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CloneID	OAc02550
Clone ID Related Accession (Same CDS sequence)	XM_002914883.3
Accession Version	XM_002914883.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-27
Organism	Ailuropoda melanoleuca(giant panda)
Product	dysferlin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003217356.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 28, 2016 this sequence version replaced XM_002914883.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ailuropoda melanoleuca Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTATGCCGAG AATGTCCACA CCCCGGACAC CGACATCAGC 
GATGCCTACT GCTCTGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CAGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGAGCTCCG AGCTCCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
TTAGGGGAAG CCCAAGTCCC ACTCCGGGAG GTCCTCGCCA CCCCCAGTCT GTCTGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CGCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCCTCTGC ATCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATATG GTGGCTGGTG GGGGACAGAG CCGGGCTGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCTG 
GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAGGACC AGGGGCTCAC GGGAGATGAG 
GCAGAGCCAT TACTGGATCC GAGCGGTGCC CCAGGCCCTG GGGCCCCCAC CACCCCGAGG 
AAGCCACCTT CCCATCCGCC CCCCTACCAC CCCGGGAGCA AGAGAAAAAG AGGTGCTCCC 
ACGCCCAGAA AGCTGCTGTC GGACAAACCG CAGGACTTCC AGATCAGGGT GCAAGTGATC 
GAGGGGCGCC AGCTGCCAGG GGTGAATATC AAGCCTGTGG TCAAAGTCAC GGCTGCCGGG 
CAGACCAAGA GGACACGGAT CCACAAGGGA AACAGCCCAC TCTTCAACGA GACTCTCTTC 
TTCAATGTGT TTGACTCTCC CTCGGAGCTG TTTGACGAGC CCATCTTTAT CACGGTGGTA 
GACTCCCGTT CTCTCCGGAC AGACGCTCTC ATTGGGGAAT TCCGGATGGA CGTGGGTACC 
ATCTACAGAG AGCCCCGACA CGCCTATCTG AGGAAATGGC TTCTCCTCTC GGACCCTGAC 
GATTTCTCTG CTGGGGCCAG AGGTTACCTG AAAGCAAGCC TCTGTGTGCT GGGGCCCGGC 
GATGAAGCGC CTCTGGAGAG AAAGGACCCC TCTGAAGACA AGGAGGACAT CGAAAACAAT 
CTGCTCAGGC CAACTGGCGT GGCCCTTCGA GGAGCCCACT TCTGCCTGAA GGTCTTCAGA 
GCTGAGGACT TACCACAGAT GGATGATGCC GTGATGGACA ACGTGAAGCA GATCTTCGGC 
TTTGACAGTA ACAAGAAGAA CTTGGTGGAT CCCTTCATGG AAGTCAGCTT TGCGGGGAAA 
ATGCTCTGCA GCAAGATCCT GGAGAAAACA GCCAACCCTC AGTGGAACCA GAGCATCACG 
CTGCCCGCCA TGTTTCCCTC CATGTGTGAA AAAATGAGGA TTCGCGTCAT GGACTGGGAC 
CGCCTCACCC ACAATGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCCGCC 
CCTGGAGGAG AAATAGAAGT GGACGACCAT CTGGGCTTCC TCCCCACCTT CGGGCCCTGC 
TACGTCAACC TCTACGGCAG CCCCAGGGAG TTCACCGGCT TCCCGGACCC CTACGCAGAA 
CTCAACATGG GCAAGGGAGA AGGTGTGGCC TACCGAGGTC GGCTTCTGCT CTCCCTGGAG 
ACCAAGCTGG TGGAGCACAG CGACCAAAAG GTGGAGGACC TCCCTGCGGA TGACATCCTT 
CGGGTGGAGA AGTATCTCCG GAGGCGTAAG TACTCCCTCT TTGCCGCCTT CTACTCGGCC 
TCCATGCTCC AGGATGTGGA TGATGCCATC CAGTTCGAGG TCAGCATCGG GAACTATGGC 
AACAAGTTCG ACACCACGTG CCTGCCGCTG GCCTCTACCA CCCAGTACAG CCGGGCGGTC 
TTTGACGGGT GCCACTACTA CTACTTGCCC TGGGGCAACG TGAAGCCCGT GGTGGTGCTG 
TCATCCTACT GGGAGGACAT CAGCCACAGA GTTGAGGCTC AGAACCAGCT GCTCAGGATT 
GCGGACCGGC TGGAAGCTGC CCTGGAGCAA GTCCACTTGG CCGTGAAGGC GCAGTACAAC 
GCCGAGGACG TGGACTCCCT GTTGGCTCAG CTGATGGATG AGCTCATCTC AGACTGCAGC 
CAGCCCCTAG GCGATGTCCA CGAGACACCC TCTGCCACCC ACCTGGACCA GTACCTGCAC 
AGGCTGCGTA GCCGTCACCT AAGCCAGATT GCAGAGGCGG CGCTGGCCCT GAAGCTCGGG 
CACGGCGGGC TCCCGGCCGC CCTGGAGCAG GCTGAAGACT GGCTCCTGCG GCTCCGTGCC 
CTGGCCGAGG AGCCTCAGAA CAGCCTGCCC GACATCATCA TCTGGATGCT GCAGGGCGAC 
AAGCGAGTGG CATACCAGCG GGTGCCTACA CATGAGGTCC TCTTCTCCCG GCGTGGAGCC 
AGCTACTGCG GCAAGAACTG TGGGAAGCTG CAGACGATAT TTCTCAAATA TCCCATGCAG 
GGGGTGCCCG GAGCCCGGAT GCCCGTGCAG ATACGGGTGA AGCTCTGGTT TGGGCTTTCT 
GTGGATGAGA AGGAGTTCAA CCAGTTTGCT GAGGGAAAGC TGTCTGTCTT TGCTGAAACC 
TATGAGAACC AGACCAAACT GGCCCTCGTT GGGAACTGGG GCACAACAGG CCTTACCTAC 
CCCAAGTTTT CTGATGTCGC GGGCAAGATC AAGCTCCCCA AGGATAGCTT CCGCCCCTCA 
GCCGGCTGGA CCTGGGCTGG AGATTGGTTT GTGTGTCCAG AGAAGACGCT GCTCCATGAC 
ACCGACGCGG GCCACCTGAG TTTCGTGGCG GAGGTGTTTG AGAACCAGAC CCGGCTCCCT 
GGAGGCCACT GGGTCTACAT GAGTGACAAC TACACGGATG TGAATGGGGA GAAGGTGCTT 
CCCAAGGATG ACATTGAGTG CCCGCTGGGC TGGAAGTGGG AAGATGAAGA GTGGTCCACG 
GACCTCAATC GGGCCGTTGA TGAGCAAGGC TGGGAGTACA GTATCACCAT CCCCCCGGAC 
CGGAAGCCGA AGCACTGGGT CCCCGCTGAG AAGATGTACT ACACGCACCG TCGGCGGCGC 
TGGGTCCGCC TGCGCAGGAG GGACCTCAGC CAGATGGAGG CCCTGAGGAA GCACAGGCAG 
GCGGAGGCGG AGGGCGAGGG CTGGGAGTAC GCCTCGCTCT TTGGCTGGAA ATTCCACCTG 
GAGTACCGCA AAACGGATGC ATTCCGTCGC CGCCGCTGGC GCCGCCGCAT GGAGCCGCTG 
GAGAAGACGG GGCCTGCAGC CGTGTTTGCC CTTGAGGGGG CACTGGGTGG CGTGGTAGAT 
GACCGGAGTG AAGATTCCAT GTCCGTGTCC ACCTTGAGCT TTGGCGTGAA CAGACCCACG 
ATTTCCTGCA TCTTTGATCA TGGGAACCGC TACCACCTGC GCTGCTATAT GTACCAGGCT 
CGGGACCTGC CCTCGATGGA CAAGGACTCT TTTTCTGATC CCTACGCCAT CGTCTCCTTC 
CTGCACCAGA GCCAGAAGAC CGTGGTGGTG AAGAACACTC TGAACCCCAC CTGGGACCAG 
ACGCTCATCT TCTATGAGAT TGAGATCTTT GGAGAGCCAG CCAGCATCGC CGAGCAGCCG 
CCCAGCATTG TGGTGGAGCT GTATGACCAC GACACCTACG GTGCAGATGA GTTTTTGGGA 
CGCTGCATGT GTCAGCCCAG TCTGGAACGG ATGCCCCGGC TGGCCTGGTT CCCGCTGGTT 
AGGGGCAGCC AGCCGGCAGG CGAGCTGCTG GCCTCTTTTG AGCTCATCCA GAGAGAGAAG 
CCGGCCATTC ACCATATTCC TGGTTTTGAG GTGCAGGATA CATCGGGAAT CCTGGACGAG 
TCGGAGGACA CAGACCTGCC CTACCCACCG CCTCAGAGGG AGGCCAACAT CTACATGGTT 
CCCCAGAACA TCAAGCCGGT TCTCCAGCGC ACTGCCATAG AGATCCTGGC ATGGGGCCTA 
CGGAACATGA AGAGTTACCA GCTGGCCAGT ATCTCCTCCC CCAGCCTCGT GGTGGAGTGC 
GGGGGCCAGA CGGTGCAGTC CTGTGTCATC AGGAACCTCC GGAAGAACCC CAACTTCGAC 
ATCTGCACCC TCTTCATGGA AGTGATGCTG CCCAGAGAGG AGCTCTACTG TCCCCCCATC 
GTGATCAAGG TCATTGATAA CCGCCAGTTT GGCCGCCGGC CTGTGGTGGG CCAGTGTACC 
ATACGCTCCC TGGAGAGCTT CATGTGTGAC CTGTACTCCG AGGAGAGTCC GTCCCCACAG 
GGCGGCCCAG ATGATGTGAG TTTACTCAGT CCTGGGGAAG ACGTGCTCAT TGACATCGAC 
GACAAGGAGC CCCTCATCCC CATACAGCTT ACAGACGGTC TGTTGGGCTC TGCCCCCACT 
AACATGGCAT CCTCTCCATC CAGTCTTCAT GAGGAGGAGT TCATCGACTG GTGGAGCAAA 
TTCTTTGCCT CCACAGGGGA GAGGGAGAAG TGCGGCTCGT ACTTGGAGAA GGGCTTTGAC 
ACTCTAAAGG TCTATGACAC ACAACTGGAG AACGTGGAGG CCTTTGGGGG CCTGTCTGAC 
TTTTGTAACA CCTTCAAGCT CTACCGGGGC AAGACTCAGG AGGAGACGGA GGACCCATCT 
GTTATCGGGG AGTTTAAGGG CCTCTTCAAA ATCTACCCCC TCCCGGAAGA CCCGGCCGTC 
CCCATGCCCC CGAGACAGTT CCAGCAGCTG GCTGCCCAGG GGCCTCAGGA CTGCCTGGTC 
CGCATCTATA TCATCCGGGC ATTTGGCCTG CAGCCCAAGG ACCCCAATGG GAAGTGTGAT 
CCTTACATCA AGATCTCCAT AGGGAAGAAA TCAGTGAGTG ACCAGGATAA TTACATTCCC 
TGTACCCTGG AGCCTGTGTT TGGAAAGATG TTCGAGCTGA CCTGCACTCT GCCTCTGGAG 
AAGGACCTGA AGATCACACT CTACGACTAT GACCTCCTCT CCAAAGATGA GAAGATCGGG 
GAGACAGTCA TCGACCTGGA GAATAGGCTG CTGTCCAAGT TCGGGGCTCG CTGCGGACTC 
CCACAGACCT ACTGTGTCTC TGGACCCAAC CAGTGGAGGG ACCAGCTCCG CCCCTCCCAG 
CTTCTCCAGC TCACCTGCCA GCAGCGCAGA CTCAAGGCGC CCGTGTACCT CACAGACCGC 
GTACTCTTTC AGGATAAAGA ATACACCATT GAGGAAATAG AGGCTGGCAG GGTCCTGAAC 
CCACACCTGG GCCCAGTGGA AGAGCGTCTG GCCTTGCACG TCCTCCAGCA GCTGGGCCTG 
GTCCCCGAGC ATGTGGAGTC CCGGCCTCTT TACAGCCCCC TGCAGCCAGA CATTGAGCAG 
GGGAAGCTGC AGATGTGGAT CGATCTGTTT CCCAAGGCCC TGGGGCGACC TGGACCTCCC 
TTCAACATCA CCCCACGGAG AGCAAGAAGG TTTTTCCTGC GTTGTATTAT CTGGAACACA 
AAGGACGTGA TCCTGGACGA CCTCAGCATC ACGGGGGAGA AGATGAGTGA CATTTACGTG 
AAAGGTTGGA TGGTCGGCTT TGAAGAGCAC AAGCAGAAGA CGGACGTGCA CTACCGCTCC 
CTGGGAGGCG AAGGCAACTT TAACTGGAGG TTCATTTTCC CCTTTGACTA CCTGCCCGCT 
GAGCAAGTCT GCACCGTCTC TAAGAAGGAT GCTTTCTGGA GGCTGGACAA GACTGAGAGC 
AAAATCCCAG CACAAGTGGT ATTTCAGATC TGGGACAATG ACAAGTTCTC CTTTGACGAC 
TTTCTGGGCT CGTTGCAGTT GGATCTCAAC CGCATGCCCA AGCCTGCCAA GACAGCTGAG 
AAGTGCTCCT TGGACCAGCT GGACGACACT TTCCACCCGG AGTGGTTTGT GTCCCTTTTT 
GAACAGAAAA CAGTAAAGGG CTGGTGGCCC TGTGTGGCAG AAGACGGTGA GAAGAAGATC 
CTGGCGGGCA AGCTGGAAAT GACCTTGGAG ATCGTAGCAG AGAGTGAGCA CGAAGAGCGG 
CCTGCGGGTC AAGGCCGGGA TGAACCCAAC ATGAACCCCA AGCTCGAGGA CCCAAGGCGC 
CCCGACACCT CCTTCCTGTG GTTCACCTCT CCGTACAAGA CCATGAAGTT CATCCTGTGG 
CGGCGGTTCC GGTGCGCCAT CATCCTCTTC GTCGTTCTCG TCATCCTGCT GCTCTTCCTG 
GGCATCTTCC TCTATTCCTT CCCGAACTAT GCTGCCATGA AGCTGGTGAA ACCCTTCAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002914929.1
CDS	487..6849
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEAQVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPSASLEPSPTLPDMDMVAGGGQSRAETWSLLSDSTVDTRYSGKKWLAPTDTGGEEDTEDQGLTGDEAEPLLDPSGAPGPGAPTTPRKPPSHPPPYHPGSKRKRGAPTPRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVMDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDHLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRLLLSLETKLVEHSDQKVEDLPADDILRVEKYLRRRKYSLFAAFYSASMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADRLEAALEQVHLAVKAQYNAEDVDSLLAQLMDELISDCSQPLGDVHETPSATHLDQYLHRLRSRHLSQIAEAALALKLGHGGLPAALEQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPTHEVLFSRRGASYCGKNCGKLQTIFLKYPMQGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVAGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDTDAGHLSFVAEVFENQTRLPGGHWVYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALRKHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSMSVSTLSFGVNRPTISCIFDHGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFLGRCMCQPSLERMPRLAWFPLVRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTSGILDESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPIVIKVIDNRQFGRRPVVGQCTIRSLESFMCDLYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLTDGLLGSAPTNMASSPSSLHEEEFIDWWSKFFASTGEREKCGSYLEKGFDTLKVYDTQLENVEAFGGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAVPMPPRQFQQLAAQGPQDCLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLQLTCQQRRLKAPVYLTDRVLFQDKEYTIEEIEAGRVLNPHLGPVEERLALHVLQQLGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPAQVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEDGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFVVLVILLLFLGIFLYSFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_002914883.3
Organism	Ailuropoda melanoleuca(giant panda)
Definition	Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002914883.3

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCCGAG AATGTCCACA CCCCGGACAC CGACATCAGC 
GATGCCTACT GCTCTGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CAGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGAGCTCCG AGCTCCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
TTAGGGGAAG CCCAAGTCCC ACTCCGGGAG GTCCTCGCCA CCCCCAGTCT GTCTGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CGCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCCTCTGC ATCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATATG GTGGCTGGTG GGGGACAGAG CCGGGCTGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCTG 
GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAGGACC AGGGGCTCAC GGGAGATGAG 
GCAGAGCCAT TACTGGATCC GAGCGGTGCC CCAGGCCCTG GGGCCCCCAC CACCCCGAGG 
AAGCCACCTT CCCATCCGCC CCCCTACCAC CCCGGGAGCA AGAGAAAAAG AGGTGCTCCC 
ACGCCCAGAA AGCTGCTGTC GGACAAACCG CAGGACTTCC AGATCAGGGT GCAAGTGATC 
GAGGGGCGCC AGCTGCCAGG GGTGAATATC AAGCCTGTGG TCAAAGTCAC GGCTGCCGGG 
CAGACCAAGA GGACACGGAT CCACAAGGGA AACAGCCCAC TCTTCAACGA GACTCTCTTC 
TTCAATGTGT TTGACTCTCC CTCGGAGCTG TTTGACGAGC CCATCTTTAT CACGGTGGTA 
GACTCCCGTT CTCTCCGGAC AGACGCTCTC ATTGGGGAAT TCCGGATGGA CGTGGGTACC 
ATCTACAGAG AGCCCCGACA CGCCTATCTG AGGAAATGGC TTCTCCTCTC GGACCCTGAC 
GATTTCTCTG CTGGGGCCAG AGGTTACCTG AAAGCAAGCC TCTGTGTGCT GGGGCCCGGC 
GATGAAGCGC CTCTGGAGAG AAAGGACCCC TCTGAAGACA AGGAGGACAT CGAAAACAAT 
CTGCTCAGGC CAACTGGCGT GGCCCTTCGA GGAGCCCACT TCTGCCTGAA GGTCTTCAGA 
GCTGAGGACT TACCACAGAT GGATGATGCC GTGATGGACA ACGTGAAGCA GATCTTCGGC 
TTTGACAGTA ACAAGAAGAA CTTGGTGGAT CCCTTCATGG AAGTCAGCTT TGCGGGGAAA 
ATGCTCTGCA GCAAGATCCT GGAGAAAACA GCCAACCCTC AGTGGAACCA GAGCATCACG 
CTGCCCGCCA TGTTTCCCTC CATGTGTGAA AAAATGAGGA TTCGCGTCAT GGACTGGGAC 
CGCCTCACCC ACAATGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCCGCC 
CCTGGAGGAG AAATAGAAGT GGACGACCAT CTGGGCTTCC TCCCCACCTT CGGGCCCTGC 
TACGTCAACC TCTACGGCAG CCCCAGGGAG TTCACCGGCT TCCCGGACCC CTACGCAGAA 
CTCAACATGG GCAAGGGAGA AGGTGTGGCC TACCGAGGTC GGCTTCTGCT CTCCCTGGAG 
ACCAAGCTGG TGGAGCACAG CGACCAAAAG GTGGAGGACC TCCCTGCGGA TGACATCCTT 
CGGGTGGAGA AGTATCTCCG GAGGCGTAAG TACTCCCTCT TTGCCGCCTT CTACTCGGCC 
TCCATGCTCC AGGATGTGGA TGATGCCATC CAGTTCGAGG TCAGCATCGG GAACTATGGC 
AACAAGTTCG ACACCACGTG CCTGCCGCTG GCCTCTACCA CCCAGTACAG CCGGGCGGTC 
TTTGACGGGT GCCACTACTA CTACTTGCCC TGGGGCAACG TGAAGCCCGT GGTGGTGCTG 
TCATCCTACT GGGAGGACAT CAGCCACAGA GTTGAGGCTC AGAACCAGCT GCTCAGGATT 
GCGGACCGGC TGGAAGCTGC CCTGGAGCAA GTCCACTTGG CCGTGAAGGC GCAGTACAAC 
GCCGAGGACG TGGACTCCCT GTTGGCTCAG CTGATGGATG AGCTCATCTC AGACTGCAGC 
CAGCCCCTAG GCGATGTCCA CGAGACACCC TCTGCCACCC ACCTGGACCA GTACCTGCAC 
AGGCTGCGTA GCCGTCACCT AAGCCAGATT GCAGAGGCGG CGCTGGCCCT GAAGCTCGGG 
CACGGCGGGC TCCCGGCCGC CCTGGAGCAG GCTGAAGACT GGCTCCTGCG GCTCCGTGCC 
CTGGCCGAGG AGCCTCAGAA CAGCCTGCCC GACATCATCA TCTGGATGCT GCAGGGCGAC 
AAGCGAGTGG CATACCAGCG GGTGCCTACA CATGAGGTCC TCTTCTCCCG GCGTGGAGCC 
AGCTACTGCG GCAAGAACTG TGGGAAGCTG CAGACGATAT TTCTCAAATA TCCCATGCAG 
GGGGTGCCCG GAGCCCGGAT GCCCGTGCAG ATACGGGTGA AGCTCTGGTT TGGGCTTTCT 
GTGGATGAGA AGGAGTTCAA CCAGTTTGCT GAGGGAAAGC TGTCTGTCTT TGCTGAAACC 
TATGAGAACC AGACCAAACT GGCCCTCGTT GGGAACTGGG GCACAACAGG CCTTACCTAC 
CCCAAGTTTT CTGATGTCGC GGGCAAGATC AAGCTCCCCA AGGATAGCTT CCGCCCCTCA 
GCCGGCTGGA CCTGGGCTGG AGATTGGTTT GTGTGTCCAG AGAAGACGCT GCTCCATGAC 
ACCGACGCGG GCCACCTGAG TTTCGTGGCG GAGGTGTTTG AGAACCAGAC CCGGCTCCCT 
GGAGGCCACT GGGTCTACAT GAGTGACAAC TACACGGATG TGAATGGGGA GAAGGTGCTT 
CCCAAGGATG ACATTGAGTG CCCGCTGGGC TGGAAGTGGG AAGATGAAGA GTGGTCCACG 
GACCTCAATC GGGCCGTTGA TGAGCAAGGC TGGGAGTACA GTATCACCAT CCCCCCGGAC 
CGGAAGCCGA AGCACTGGGT CCCCGCTGAG AAGATGTACT ACACGCACCG TCGGCGGCGC 
TGGGTCCGCC TGCGCAGGAG GGACCTCAGC CAGATGGAGG CCCTGAGGAA GCACAGGCAG 
GCGGAGGCGG AGGGCGAGGG CTGGGAGTAC GCCTCGCTCT TTGGCTGGAA ATTCCACCTG 
GAGTACCGCA AAACGGATGC ATTCCGTCGC CGCCGCTGGC GCCGCCGCAT GGAGCCGCTG 
GAGAAGACGG GGCCTGCAGC CGTGTTTGCC CTTGAGGGGG CACTGGGTGG CGTGGTAGAT 
GACCGGAGTG AAGATTCCAT GTCCGTGTCC ACCTTGAGCT TTGGCGTGAA CAGACCCACG 
ATTTCCTGCA TCTTTGATCA TGGGAACCGC TACCACCTGC GCTGCTATAT GTACCAGGCT 
CGGGACCTGC CCTCGATGGA CAAGGACTCT TTTTCTGATC CCTACGCCAT CGTCTCCTTC 
CTGCACCAGA GCCAGAAGAC CGTGGTGGTG AAGAACACTC TGAACCCCAC CTGGGACCAG 
ACGCTCATCT TCTATGAGAT TGAGATCTTT GGAGAGCCAG CCAGCATCGC CGAGCAGCCG 
CCCAGCATTG TGGTGGAGCT GTATGACCAC GACACCTACG GTGCAGATGA GTTTTTGGGA 
CGCTGCATGT GTCAGCCCAG TCTGGAACGG ATGCCCCGGC TGGCCTGGTT CCCGCTGGTT 
AGGGGCAGCC AGCCGGCAGG CGAGCTGCTG GCCTCTTTTG AGCTCATCCA GAGAGAGAAG 
CCGGCCATTC ACCATATTCC TGGTTTTGAG GTGCAGGATA CATCGGGAAT CCTGGACGAG 
TCGGAGGACA CAGACCTGCC CTACCCACCG CCTCAGAGGG AGGCCAACAT CTACATGGTT 
CCCCAGAACA TCAAGCCGGT TCTCCAGCGC ACTGCCATAG AGATCCTGGC ATGGGGCCTA 
CGGAACATGA AGAGTTACCA GCTGGCCAGT ATCTCCTCCC CCAGCCTCGT GGTGGAGTGC 
GGGGGCCAGA CGGTGCAGTC CTGTGTCATC AGGAACCTCC GGAAGAACCC CAACTTCGAC 
ATCTGCACCC TCTTCATGGA AGTGATGCTG CCCAGAGAGG AGCTCTACTG TCCCCCCATC 
GTGATCAAGG TCATTGATAA CCGCCAGTTT GGCCGCCGGC CTGTGGTGGG CCAGTGTACC 
ATACGCTCCC TGGAGAGCTT CATGTGTGAC CTGTACTCCG AGGAGAGTCC GTCCCCACAG 
GGCGGCCCAG ATGATGTGAG TTTACTCAGT CCTGGGGAAG ACGTGCTCAT TGACATCGAC 
GACAAGGAGC CCCTCATCCC CATACAGCTT ACAGACGGTC TGTTGGGCTC TGCCCCCACT 
AACATGGCAT CCTCTCCATC CAGTCTTCAT GAGGAGGAGT TCATCGACTG GTGGAGCAAA 
TTCTTTGCCT CCACAGGGGA GAGGGAGAAG TGCGGCTCGT ACTTGGAGAA GGGCTTTGAC 
ACTCTAAAGG TCTATGACAC ACAACTGGAG AACGTGGAGG CCTTTGGGGG CCTGTCTGAC 
TTTTGTAACA CCTTCAAGCT CTACCGGGGC AAGACTCAGG AGGAGACGGA GGACCCATCT 
GTTATCGGGG AGTTTAAGGG CCTCTTCAAA ATCTACCCCC TCCCGGAAGA CCCGGCCGTC 
CCCATGCCCC CGAGACAGTT CCAGCAGCTG GCTGCCCAGG GGCCTCAGGA CTGCCTGGTC 
CGCATCTATA TCATCCGGGC ATTTGGCCTG CAGCCCAAGG ACCCCAATGG GAAGTGTGAT 
CCTTACATCA AGATCTCCAT AGGGAAGAAA TCAGTGAGTG ACCAGGATAA TTACATTCCC 
TGTACCCTGG AGCCTGTGTT TGGAAAGATG TTCGAGCTGA CCTGCACTCT GCCTCTGGAG 
AAGGACCTGA AGATCACACT CTACGACTAT GACCTCCTCT CCAAAGATGA GAAGATCGGG 
GAGACAGTCA TCGACCTGGA GAATAGGCTG CTGTCCAAGT TCGGGGCTCG CTGCGGACTC 
CCACAGACCT ACTGTGTCTC TGGACCCAAC CAGTGGAGGG ACCAGCTCCG CCCCTCCCAG 
CTTCTCCAGC TCACCTGCCA GCAGCGCAGA CTCAAGGCGC CCGTGTACCT CACAGACCGC 
GTACTCTTTC AGGATAAAGA ATACACCATT GAGGAAATAG AGGCTGGCAG GGTCCTGAAC 
CCACACCTGG GCCCAGTGGA AGAGCGTCTG GCCTTGCACG TCCTCCAGCA GCTGGGCCTG 
GTCCCCGAGC ATGTGGAGTC CCGGCCTCTT TACAGCCCCC TGCAGCCAGA CATTGAGCAG 
GGGAAGCTGC AGATGTGGAT CGATCTGTTT CCCAAGGCCC TGGGGCGACC TGGACCTCCC 
TTCAACATCA CCCCACGGAG AGCAAGAAGG TTTTTCCTGC GTTGTATTAT CTGGAACACA 
AAGGACGTGA TCCTGGACGA CCTCAGCATC ACGGGGGAGA AGATGAGTGA CATTTACGTG 
AAAGGTTGGA TGGTCGGCTT TGAAGAGCAC AAGCAGAAGA CGGACGTGCA CTACCGCTCC 
CTGGGAGGCG AAGGCAACTT TAACTGGAGG TTCATTTTCC CCTTTGACTA CCTGCCCGCT 
GAGCAAGTCT GCACCGTCTC TAAGAAGGAT GCTTTCTGGA GGCTGGACAA GACTGAGAGC 
AAAATCCCAG CACAAGTGGT ATTTCAGATC TGGGACAATG ACAAGTTCTC CTTTGACGAC 
TTTCTGGGCT CGTTGCAGTT GGATCTCAAC CGCATGCCCA AGCCTGCCAA GACAGCTGAG 
AAGTGCTCCT TGGACCAGCT GGACGACACT TTCCACCCGG AGTGGTTTGT GTCCCTTTTT 
GAACAGAAAA CAGTAAAGGG CTGGTGGCCC TGTGTGGCAG AAGACGGTGA GAAGAAGATC 
CTGGCGGGCA AGCTGGAAAT GACCTTGGAG ATCGTAGCAG AGAGTGAGCA CGAAGAGCGG 
CCTGCGGGTC AAGGCCGGGA TGAACCCAAC ATGAACCCCA AGCTCGAGGA CCCAAGGCGC 
CCCGACACCT CCTTCCTGTG GTTCACCTCT CCGTACAAGA CCATGAAGTT CATCCTGTGG 
CGGCGGTTCC GGTGCGCCAT CATCCTCTTC GTCGTTCTCG TCATCCTGCT GCTCTTCCTG 
GGCATCTTCC TCTATTCCTT CCCGAACTAT GCTGCCATGA AGCTGGTGAA ACCCTTCAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_002914885.3 ) cDNA ORF clone, Ailuropoda melanoleuca(giant panda) -> XP_002914931.1 Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X15, mRNA.

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CloneID	OAc02552
Clone ID Related Accession (Same CDS sequence)	XM_002914885.3
Accession Version	XM_002914885.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-27
Organism	Ailuropoda melanoleuca(giant panda)
Product	dysferlin isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003217356.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 28, 2016 this sequence version replaced XM_002914885.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ailuropoda melanoleuca Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTATGCCGAG AATGTCCACA CCCCGGACAC CGACATCAGC 
GATGCCTACT GCTCTGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CAGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGAGCTCCG AGCTCCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
TTAGGGGAAG CCCAAGTCCC ACTCCGGGAG GTCCTCGCCA CCCCCAGTCT GTCTGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CGCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCCTCTGC ATCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATATG GTGGCTGGTG GGGGACAGAG CCGGGCTGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCTG 
GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAGGACC AGGGGCTCAC GGGAGATGAG 
GCAGAGCCAT TACTGGATCC GAGCGGTGCC CCAGGCCCTG GGGCCCCCAC CACCCCGAGG 
AAGCCACCTT CCCATCCGCC CCCCTACCAC CCCGGGAGCA AGAGAAAAAG AGGTGCTCCC 
ACGCCCAGAA AGCTGCTGTC GGACAAACCG CAGGACTTCC AGATCAGGGT GCAAGTGATC 
GAGGGGCGCC AGCTGCCAGG GGTGAATATC AAGCCTGTGG TCAAAGTCAC GGCTGCCGGG 
CAGACCAAGA GGACACGGAT CCACAAGGGA AACAGCCCAC TCTTCAACGA GACTCTCTTC 
TTCAATGTGT TTGACTCTCC CTCGGAGCTG TTTGACGAGC CCATCTTTAT CACGGTGGTA 
GACTCCCGTT CTCTCCGGAC AGACGCTCTC ATTGGGGAAT TCCGGATGGA CGTGGGTACC 
ATCTACAGAG AGCCCCGACA CGCCTATCTG AGGAAATGGC TTCTCCTCTC GGACCCTGAC 
GATTTCTCTG CTGGGGCCAG AGGTTACCTG AAAGCAAGCC TCTGTGTGCT GGGGCCCGGC 
GATGAAGCGC CTCTGGAGAG AAAGGACCCC TCTGAAGACA AGGAGGACAT CGAAAACAAT 
CTGCTCAGGC CAACTGGCGT GGCCCTTCGA GGAGCCCACT TCTGCCTGAA GGTCTTCAGA 
GCTGAGGACT TACCACAGAT GGATGATGCC GTGATGGACA ACGTGAAGCA GATCTTCGGC 
TTTGACAGTA ACAAGAAGAA CTTGGTGGAT CCCTTCATGG AAGTCAGCTT TGCGGGGAAA 
ATGCTCTGCA GCAAGATCCT GGAGAAAACA GCCAACCCTC AGTGGAACCA GAGCATCACG 
CTGCCCGCCA TGTTTCCCTC CATGTGTGAA AAAATGAGGA TTCGCGTCAT GGACTGGGAC 
CGCCTCACCC ACAATGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCCGCC 
CCTGGAGGAG AAATAGAAGT GGACGACCAT CTGGGCTTCC TCCCCACCTT CGGGCCCTGC 
TACGTCAACC TCTACGGCAG CCCCAGGGAG TTCACCGGCT TCCCGGACCC CTACGCAGAA 
CTCAACATGG GCAAGGGAGA AGGTGTGGCC TACCGAGGTC GGCTTCTGCT CTCCCTGGAG 
ACCAAGCTGG TGGAGCACAG CGACCAAAAG GTGGAGGACC TCCCTGCGGA TGACATCCTT 
CGGGTGGAGA AGTATCTCCG GAGGCGTAAG TACTCCCTCT TTGCCGCCTT CTACTCGGCC 
TCCATGCTCC AGGATGTGGA TGATGCCATC CAGTTCGAGG TCAGCATCGG GAACTATGGC 
AACAAGTTCG ACACCACGTG CCTGCCGCTG GCCTCTACCA CCCAGTACAG CCGGGCGGTC 
TTTGACGGGT GCCACTACTA CTACTTGCCC TGGGGCAACG TGAAGCCCGT GGTGGTGCTG 
TCATCCTACT GGGAGGACAT CAGCCACAGA GTTGAGGCTC AGAACCAGCT GCTCAGGATT 
GCGGACCGGC TGGAAGCTGC CCTGGAGCAA GTCCACTTGG CCGTGAAGGC GCAGTACAAC 
GCCGAGGACG TGGACTCCCT GTTGGCTCAG CTGATGGATG AGCTCATCTC AGACTGCAGC 
CAGCCCCTAG GCGATGTCCA CGAGACACCC TCTGCCACCC ACCTGGACCA GTACCTGCAC 
AGGCTGCGTA GCCGTCACCT AAGCCAGATT GCAGAGGCGG CGCTGGCCCT GAAGCTCGGG 
CACGGCGGGC TCCCGGCCGC CCTGGAGCAG GCTGAAGACT GGCTCCTGCG GCTCCGTGCC 
CTGGCCGAGG AGCCTCAGAA CAGCCTGCCC GACATCATCA TCTGGATGCT GCAGGGCGAC 
AAGCGAGTGG CATACCAGCG GGTGCCTACA CATGAGGTCC TCTTCTCCCG GCGTGGAGCC 
AGCTACTGCG GCAAGAACTG TGGGAAGCTG CAGACGATAT TTCTCAAATA TCCCATGCAG 
GGGGTGCCCG GAGCCCGGAT GCCCGTGCAG ATACGGGTGA AGCTCTGGTT TGGGCTTTCT 
GTGGATGAGA AGGAGTTCAA CCAGTTTGCT GAGGGAAAGC TGTCTGTCTT TGCTGAAACC 
TATGAGAACC AGACCAAACT GGCCCTCGTT GGGAACTGGG GCACAACAGG CCTTACCTAC 
CCCAAGTTTT CTGATGTCGC GGGCAAGATC AAGCTCCCCA AGGATAGCTT CCGCCCCTCA 
GCCGGCTGGA CCTGGGCTGG AGATTGGTTT GTGTGTCCAG AGAAGACGCT GCTCCATGAC 
ACCGACGCGG GCCACCTGAG TTTCGTGGCG GAGGTGTTTG AGAACCAGAC CCGGCTCCCT 
GGAGGCCACT GGGTCTACAT GAGTGACAAC TACACGGATG TGAATGGGGA GAAGGTGCTT 
CCCAAGGATG ACATTGAGTG CCCGCTGGGC TGGAAGTGGG AAGATGAAGA GTGGTCCACG 
GACCTCAATC GGGCCGTTGA TGAGCAAGGC TGGGAGTACA GTATCACCAT CCCCCCGGAC 
CGGAAGCCGA AGCACTGGGT CCCCGCTGAG AAGATGTACT ACACGCACCG TCGGCGGCGC 
TGGGTCCGCC TGCGCAGGAG GGACCTCAGC CAGATGGAGG CCCTGAGGAA GCACAGGCAG 
GCGGAGGCGG AGGGCGAGGG CTGGGAGTAC GCCTCGCTCT TTGGCTGGAA ATTCCACCTG 
GAGTACCGCA AAACGGATGC ATTCCGTCGC CGCCGCTGGC GCCGCCGCAT GGAGCCGCTG 
GAGAAGACGG GGCCTGCAGC CGTGTTTGCC CTTGAGGGGG CACTGGGTGG CGTGGTAGAT 
GACCGGAGTG AAGATTCCAT GTCCGTGTCC ACCTTGAGCT TTGGCGTGAA CAGACCCACG 
ATTTCCTGCA TCTTTGATCA TGGGAACCGC TACCACCTGC GCTGCTATAT GTACCAGGCT 
CGGGACCTGC CCTCGATGGA CAAGGACTCT TTTTCTGATC CCTACGCCAT CGTCTCCTTC 
CTGCACCAGA GCCAGAAGAC CGTGGTGGTG AAGAACACTC TGAACCCCAC CTGGGACCAG 
ACGCTCATCT TCTATGAGAT TGAGATCTTT GGAGAGCCAG CCAGCATCGC CGAGCAGCCG 
CCCAGCATTG TGGTGGAGCT GTATGACCAC GACACCTACG GTGCAGATGA GTTTTTGGGA 
CGCTGCATGT GTCAGCCCAG TCTGGAACGG ATGCCCCGGC TGGCCTGGTT CCCGCTGGTT 
AGGGGCAGCC AGCCGGCAGG CGAGCTGCTG GCCTCTTTTG AGCTCATCCA GAGAGAGAAG 
CCGGCCATTC ACCATATTCC TGGTTTTGAG GTGCAGGATA CATCGGGAAT CCTGGACGAG 
TCGGAGGACA CAGACCTGCC CTACCCACCG CCTCAGAGGG AGGCCAACAT CTACATGGTT 
CCCCAGAACA TCAAGCCGGT TCTCCAGCGC ACTGCCATAG AGATCCTGGC ATGGGGCCTA 
CGGAACATGA AGAGTTACCA GCTGGCCAGT ATCTCCTCCC CCAGCCTCGT GGTGGAGTGC 
GGGGGCCAGA CGGTGCAGTC CTGTGTCATC AGGAACCTCC GGAAGAACCC CAACTTCGAC 
ATCTGCACCC TCTTCATGGA AGTGATGCTG CCCAGAGAGG AGCTCTACTG TCCCCCCATC 
GTGATCAAGG TCATTGATAA CCGCCAGTTT GGCCGCCGGC CTGTGGTGGG CCAGTGTACC 
ATACGCTCCC TGGAGAGCTT CATGTGTGAC CTGTACTCCG AGGAGAGTCC GTCCCCACAG 
GGCGGCCCAG ATGATGTGAG TTTACTCAGT CCTGGGGAAG ACGTGCTCAT TGACATCGAC 
GACAAGGAGC CCCTCATCCC CATACAGGAG GAGGAGTTCA TCGACTGGTG GAGCAAATTC 
TTTGCCTCCA CAGGGGAGAG GGAGAAGTGC GGCTCGTACT TGGAGAAGGG CTTTGACACT 
CTAAAGGTCT ATGACACACA ACTGGAGAAC GTGGAGGCCT TTGGGGGCCT GTCTGACTTT 
TGTAACACCT TCAAGCTCTA CCGGGGCAAG ACTCAGGAGG AGACGGAGGA CCCATCTGTT 
ATCGGGGAGT TTAAGGGCCT CTTCAAAATC TACCCCCTCC CGGAAGACCC GGCCGTCCCC 
ATGCCCCCGA GACAGTTCCA GCAGCTGGCT GCCCAGGGGC CTCAGGACTG CCTGGTCCGC 
ATCTATATCA TCCGGGCATT TGGCCTGCAG CCCAAGGACC CCAATGGGAA GTGTGATCCT 
TACATCAAGA TCTCCATAGG GAAGAAATCA GTGAGTGACC AGGATAATTA CATTCCCTGT 
ACCCTGGAGC CTGTGTTTGG AAAGATGTTC GAGCTGACCT GCACTCTGCC TCTGGAGAAG 
GACCTGAAGA TCACACTCTA CGACTATGAC CTCCTCTCCA AAGATGAGAA GATCGGGGAG 
ACAGTCATCG ACCTGGAGAA TAGGCTGCTG TCCAAGTTCG GGGCTCGCTG CGGACTCCCA 
CAGACCTACT GTGTCTCTGG ACCCAACCAG TGGAGGGACC AGCTCCGCCC CTCCCAGCTT 
CTCCAGCTCA CCTGCCAGCA GCGCAGACTC AAGGCGCCCG TGTACCTCAC AGACCGCGTA 
CTCTTTCAGG ATAAAGAATA CACCATTGAG GAAATAGAGG CTGGCAGGGT CCTGAACCCA 
CACCTGGGCC CAGTGGAAGA GCGTCTGGCC TTGCACGTCC TCCAGCAGCT GGGCCTGGTC 
CCCGAGCATG TGGAGTCCCG GCCTCTTTAC AGCCCCCTGC AGCCAGACAT TGAGCAGGGG 
AAGCTGCAGA TGTGGATCGA TCTGTTTCCC AAGGCCCTGG GGCGACCTGG ACCTCCCTTC 
AACATCACCC CACGGAGAGC AAGAAGGTTT TTCCTGCGTT GTATTATCTG GAACACAAAG 
GACGTGATCC TGGACGACCT CAGCATCACG GGGGAGAAGA TGAGTGACAT TTACGTGAAA 
GGTTGGATGG TCGGCTTTGA AGAGCACAAG CAGAAGACGG ACGTGCACTA CCGCTCCCTG 
GGAGGCGAAG GCAACTTTAA CTGGAGGTTC ATTTTCCCCT TTGACTACCT GCCCGCTGAG 
CAAGTCTGCA CCGTCTCTAA GAAGGATGCT TTCTGGAGGC TGGACAAGAC TGAGAGCAAA 
ATCCCAGCAC AAGTGGTATT TCAGATCTGG GACAATGACA AGTTCTCCTT TGACGACTTT 
CTGGGCTCGT TGCAGTTGGA TCTCAACCGC ATGCCCAAGC CTGCCAAGAC AGCTGAGAAG 
TGCTCCTTGG ACCAGCTGGA CGACACTTTC CACCCGGAGT GGTTTGTGTC CCTTTTTGAA 
CAGAAAACAG TAAAGGGCTG GTGGCCCTGT GTGGCAGAAG ACGGTGAGAA GAAGATCCTG 
GCGGGCAAGC TGGAAATGAC CTTGGAGATC GTAGCAGAGA GTGAGCACGA AGAGCGGCCT 
GCGGGTCAAG GCCGGGATGA ACCCAACATG AACCCCAAGC TCGAGGACCC AAGGCGCCCC 
GACACCTCCT TCCTGTGGTT CACCTCTCCG TACAAGACCA TGAAGTTCAT CCTGTGGCGG 
CGGTTCCGGT GCGCCATCAT CCTCTTCGTC GTTCTCGTCA TCCTGCTGCT CTTCCTGGGC 
ATCTTCCTCT ATTCCTTCCC GAACTATGCT GCCATGAAGC TGGTGAAACC CTTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002914931.1
CDS	487..6786
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEAQVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPSASLEPSPTLPDMDMVAGGGQSRAETWSLLSDSTVDTRYSGKKWLAPTDTGGEEDTEDQGLTGDEAEPLLDPSGAPGPGAPTTPRKPPSHPPPYHPGSKRKRGAPTPRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVMDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDHLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRLLLSLETKLVEHSDQKVEDLPADDILRVEKYLRRRKYSLFAAFYSASMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADRLEAALEQVHLAVKAQYNAEDVDSLLAQLMDELISDCSQPLGDVHETPSATHLDQYLHRLRSRHLSQIAEAALALKLGHGGLPAALEQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPTHEVLFSRRGASYCGKNCGKLQTIFLKYPMQGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVAGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDTDAGHLSFVAEVFENQTRLPGGHWVYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALRKHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSMSVSTLSFGVNRPTISCIFDHGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFLGRCMCQPSLERMPRLAWFPLVRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTSGILDESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPIVIKVIDNRQFGRRPVVGQCTIRSLESFMCDLYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASTGEREKCGSYLEKGFDTLKVYDTQLENVEAFGGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAVPMPPRQFQQLAAQGPQDCLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLQLTCQQRRLKAPVYLTDRVLFQDKEYTIEEIEAGRVLNPHLGPVEERLALHVLQQLGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPAQVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEDGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFVVLVILLLFLGIFLYSFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_002914885.3
Organism	Ailuropoda melanoleuca(giant panda)
Definition	Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002914885.3

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCCGAG AATGTCCACA CCCCGGACAC CGACATCAGC 
GATGCCTACT GCTCTGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CAGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGAGCTCCG AGCTCCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
TTAGGGGAAG CCCAAGTCCC ACTCCGGGAG GTCCTCGCCA CCCCCAGTCT GTCTGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CGCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCCTCTGC ATCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATATG GTGGCTGGTG GGGGACAGAG CCGGGCTGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCTG 
GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAGGACC AGGGGCTCAC GGGAGATGAG 
GCAGAGCCAT TACTGGATCC GAGCGGTGCC CCAGGCCCTG GGGCCCCCAC CACCCCGAGG 
AAGCCACCTT CCCATCCGCC CCCCTACCAC CCCGGGAGCA AGAGAAAAAG AGGTGCTCCC 
ACGCCCAGAA AGCTGCTGTC GGACAAACCG CAGGACTTCC AGATCAGGGT GCAAGTGATC 
GAGGGGCGCC AGCTGCCAGG GGTGAATATC AAGCCTGTGG TCAAAGTCAC GGCTGCCGGG 
CAGACCAAGA GGACACGGAT CCACAAGGGA AACAGCCCAC TCTTCAACGA GACTCTCTTC 
TTCAATGTGT TTGACTCTCC CTCGGAGCTG TTTGACGAGC CCATCTTTAT CACGGTGGTA 
GACTCCCGTT CTCTCCGGAC AGACGCTCTC ATTGGGGAAT TCCGGATGGA CGTGGGTACC 
ATCTACAGAG AGCCCCGACA CGCCTATCTG AGGAAATGGC TTCTCCTCTC GGACCCTGAC 
GATTTCTCTG CTGGGGCCAG AGGTTACCTG AAAGCAAGCC TCTGTGTGCT GGGGCCCGGC 
GATGAAGCGC CTCTGGAGAG AAAGGACCCC TCTGAAGACA AGGAGGACAT CGAAAACAAT 
CTGCTCAGGC CAACTGGCGT GGCCCTTCGA GGAGCCCACT TCTGCCTGAA GGTCTTCAGA 
GCTGAGGACT TACCACAGAT GGATGATGCC GTGATGGACA ACGTGAAGCA GATCTTCGGC 
TTTGACAGTA ACAAGAAGAA CTTGGTGGAT CCCTTCATGG AAGTCAGCTT TGCGGGGAAA 
ATGCTCTGCA GCAAGATCCT GGAGAAAACA GCCAACCCTC AGTGGAACCA GAGCATCACG 
CTGCCCGCCA TGTTTCCCTC CATGTGTGAA AAAATGAGGA TTCGCGTCAT GGACTGGGAC 
CGCCTCACCC ACAATGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCCGCC 
CCTGGAGGAG AAATAGAAGT GGACGACCAT CTGGGCTTCC TCCCCACCTT CGGGCCCTGC 
TACGTCAACC TCTACGGCAG CCCCAGGGAG TTCACCGGCT TCCCGGACCC CTACGCAGAA 
CTCAACATGG GCAAGGGAGA AGGTGTGGCC TACCGAGGTC GGCTTCTGCT CTCCCTGGAG 
ACCAAGCTGG TGGAGCACAG CGACCAAAAG GTGGAGGACC TCCCTGCGGA TGACATCCTT 
CGGGTGGAGA AGTATCTCCG GAGGCGTAAG TACTCCCTCT TTGCCGCCTT CTACTCGGCC 
TCCATGCTCC AGGATGTGGA TGATGCCATC CAGTTCGAGG TCAGCATCGG GAACTATGGC 
AACAAGTTCG ACACCACGTG CCTGCCGCTG GCCTCTACCA CCCAGTACAG CCGGGCGGTC 
TTTGACGGGT GCCACTACTA CTACTTGCCC TGGGGCAACG TGAAGCCCGT GGTGGTGCTG 
TCATCCTACT GGGAGGACAT CAGCCACAGA GTTGAGGCTC AGAACCAGCT GCTCAGGATT 
GCGGACCGGC TGGAAGCTGC CCTGGAGCAA GTCCACTTGG CCGTGAAGGC GCAGTACAAC 
GCCGAGGACG TGGACTCCCT GTTGGCTCAG CTGATGGATG AGCTCATCTC AGACTGCAGC 
CAGCCCCTAG GCGATGTCCA CGAGACACCC TCTGCCACCC ACCTGGACCA GTACCTGCAC 
AGGCTGCGTA GCCGTCACCT AAGCCAGATT GCAGAGGCGG CGCTGGCCCT GAAGCTCGGG 
CACGGCGGGC TCCCGGCCGC CCTGGAGCAG GCTGAAGACT GGCTCCTGCG GCTCCGTGCC 
CTGGCCGAGG AGCCTCAGAA CAGCCTGCCC GACATCATCA TCTGGATGCT GCAGGGCGAC 
AAGCGAGTGG CATACCAGCG GGTGCCTACA CATGAGGTCC TCTTCTCCCG GCGTGGAGCC 
AGCTACTGCG GCAAGAACTG TGGGAAGCTG CAGACGATAT TTCTCAAATA TCCCATGCAG 
GGGGTGCCCG GAGCCCGGAT GCCCGTGCAG ATACGGGTGA AGCTCTGGTT TGGGCTTTCT 
GTGGATGAGA AGGAGTTCAA CCAGTTTGCT GAGGGAAAGC TGTCTGTCTT TGCTGAAACC 
TATGAGAACC AGACCAAACT GGCCCTCGTT GGGAACTGGG GCACAACAGG CCTTACCTAC 
CCCAAGTTTT CTGATGTCGC GGGCAAGATC AAGCTCCCCA AGGATAGCTT CCGCCCCTCA 
GCCGGCTGGA CCTGGGCTGG AGATTGGTTT GTGTGTCCAG AGAAGACGCT GCTCCATGAC 
ACCGACGCGG GCCACCTGAG TTTCGTGGCG GAGGTGTTTG AGAACCAGAC CCGGCTCCCT 
GGAGGCCACT GGGTCTACAT GAGTGACAAC TACACGGATG TGAATGGGGA GAAGGTGCTT 
CCCAAGGATG ACATTGAGTG CCCGCTGGGC TGGAAGTGGG AAGATGAAGA GTGGTCCACG 
GACCTCAATC GGGCCGTTGA TGAGCAAGGC TGGGAGTACA GTATCACCAT CCCCCCGGAC 
CGGAAGCCGA AGCACTGGGT CCCCGCTGAG AAGATGTACT ACACGCACCG TCGGCGGCGC 
TGGGTCCGCC TGCGCAGGAG GGACCTCAGC CAGATGGAGG CCCTGAGGAA GCACAGGCAG 
GCGGAGGCGG AGGGCGAGGG CTGGGAGTAC GCCTCGCTCT TTGGCTGGAA ATTCCACCTG 
GAGTACCGCA AAACGGATGC ATTCCGTCGC CGCCGCTGGC GCCGCCGCAT GGAGCCGCTG 
GAGAAGACGG GGCCTGCAGC CGTGTTTGCC CTTGAGGGGG CACTGGGTGG CGTGGTAGAT 
GACCGGAGTG AAGATTCCAT GTCCGTGTCC ACCTTGAGCT TTGGCGTGAA CAGACCCACG 
ATTTCCTGCA TCTTTGATCA TGGGAACCGC TACCACCTGC GCTGCTATAT GTACCAGGCT 
CGGGACCTGC CCTCGATGGA CAAGGACTCT TTTTCTGATC CCTACGCCAT CGTCTCCTTC 
CTGCACCAGA GCCAGAAGAC CGTGGTGGTG AAGAACACTC TGAACCCCAC CTGGGACCAG 
ACGCTCATCT TCTATGAGAT TGAGATCTTT GGAGAGCCAG CCAGCATCGC CGAGCAGCCG 
CCCAGCATTG TGGTGGAGCT GTATGACCAC GACACCTACG GTGCAGATGA GTTTTTGGGA 
CGCTGCATGT GTCAGCCCAG TCTGGAACGG ATGCCCCGGC TGGCCTGGTT CCCGCTGGTT 
AGGGGCAGCC AGCCGGCAGG CGAGCTGCTG GCCTCTTTTG AGCTCATCCA GAGAGAGAAG 
CCGGCCATTC ACCATATTCC TGGTTTTGAG GTGCAGGATA CATCGGGAAT CCTGGACGAG 
TCGGAGGACA CAGACCTGCC CTACCCACCG CCTCAGAGGG AGGCCAACAT CTACATGGTT 
CCCCAGAACA TCAAGCCGGT TCTCCAGCGC ACTGCCATAG AGATCCTGGC ATGGGGCCTA 
CGGAACATGA AGAGTTACCA GCTGGCCAGT ATCTCCTCCC CCAGCCTCGT GGTGGAGTGC 
GGGGGCCAGA CGGTGCAGTC CTGTGTCATC AGGAACCTCC GGAAGAACCC CAACTTCGAC 
ATCTGCACCC TCTTCATGGA AGTGATGCTG CCCAGAGAGG AGCTCTACTG TCCCCCCATC 
GTGATCAAGG TCATTGATAA CCGCCAGTTT GGCCGCCGGC CTGTGGTGGG CCAGTGTACC 
ATACGCTCCC TGGAGAGCTT CATGTGTGAC CTGTACTCCG AGGAGAGTCC GTCCCCACAG 
GGCGGCCCAG ATGATGTGAG TTTACTCAGT CCTGGGGAAG ACGTGCTCAT TGACATCGAC 
GACAAGGAGC CCCTCATCCC CATACAGGAG GAGGAGTTCA TCGACTGGTG GAGCAAATTC 
TTTGCCTCCA CAGGGGAGAG GGAGAAGTGC GGCTCGTACT TGGAGAAGGG CTTTGACACT 
CTAAAGGTCT ATGACACACA ACTGGAGAAC GTGGAGGCCT TTGGGGGCCT GTCTGACTTT 
TGTAACACCT TCAAGCTCTA CCGGGGCAAG ACTCAGGAGG AGACGGAGGA CCCATCTGTT 
ATCGGGGAGT TTAAGGGCCT CTTCAAAATC TACCCCCTCC CGGAAGACCC GGCCGTCCCC 
ATGCCCCCGA GACAGTTCCA GCAGCTGGCT GCCCAGGGGC CTCAGGACTG CCTGGTCCGC 
ATCTATATCA TCCGGGCATT TGGCCTGCAG CCCAAGGACC CCAATGGGAA GTGTGATCCT 
TACATCAAGA TCTCCATAGG GAAGAAATCA GTGAGTGACC AGGATAATTA CATTCCCTGT 
ACCCTGGAGC CTGTGTTTGG AAAGATGTTC GAGCTGACCT GCACTCTGCC TCTGGAGAAG 
GACCTGAAGA TCACACTCTA CGACTATGAC CTCCTCTCCA AAGATGAGAA GATCGGGGAG 
ACAGTCATCG ACCTGGAGAA TAGGCTGCTG TCCAAGTTCG GGGCTCGCTG CGGACTCCCA 
CAGACCTACT GTGTCTCTGG ACCCAACCAG TGGAGGGACC AGCTCCGCCC CTCCCAGCTT 
CTCCAGCTCA CCTGCCAGCA GCGCAGACTC AAGGCGCCCG TGTACCTCAC AGACCGCGTA 
CTCTTTCAGG ATAAAGAATA CACCATTGAG GAAATAGAGG CTGGCAGGGT CCTGAACCCA 
CACCTGGGCC CAGTGGAAGA GCGTCTGGCC TTGCACGTCC TCCAGCAGCT GGGCCTGGTC 
CCCGAGCATG TGGAGTCCCG GCCTCTTTAC AGCCCCCTGC AGCCAGACAT TGAGCAGGGG 
AAGCTGCAGA TGTGGATCGA TCTGTTTCCC AAGGCCCTGG GGCGACCTGG ACCTCCCTTC 
AACATCACCC CACGGAGAGC AAGAAGGTTT TTCCTGCGTT GTATTATCTG GAACACAAAG 
GACGTGATCC TGGACGACCT CAGCATCACG GGGGAGAAGA TGAGTGACAT TTACGTGAAA 
GGTTGGATGG TCGGCTTTGA AGAGCACAAG CAGAAGACGG ACGTGCACTA CCGCTCCCTG 
GGAGGCGAAG GCAACTTTAA CTGGAGGTTC ATTTTCCCCT TTGACTACCT GCCCGCTGAG 
CAAGTCTGCA CCGTCTCTAA GAAGGATGCT TTCTGGAGGC TGGACAAGAC TGAGAGCAAA 
ATCCCAGCAC AAGTGGTATT TCAGATCTGG GACAATGACA AGTTCTCCTT TGACGACTTT 
CTGGGCTCGT TGCAGTTGGA TCTCAACCGC ATGCCCAAGC CTGCCAAGAC AGCTGAGAAG 
TGCTCCTTGG ACCAGCTGGA CGACACTTTC CACCCGGAGT GGTTTGTGTC CCTTTTTGAA 
CAGAAAACAG TAAAGGGCTG GTGGCCCTGT GTGGCAGAAG ACGGTGAGAA GAAGATCCTG 
GCGGGCAAGC TGGAAATGAC CTTGGAGATC GTAGCAGAGA GTGAGCACGA AGAGCGGCCT 
GCGGGTCAAG GCCGGGATGA ACCCAACATG AACCCCAAGC TCGAGGACCC AAGGCGCCCC 
GACACCTCCT TCCTGTGGTT CACCTCTCCG TACAAGACCA TGAAGTTCAT CCTGTGGCGG 
CGGTTCCGGT GCGCCATCAT CCTCTTCGTC GTTCTCGTCA TCCTGCTGCT CTTCCTGGGC 
ATCTTCCTCT ATTCCTTCCC GAACTATGCT GCCATGAAGC TGGTGAAACC CTTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_002914886.3 ) cDNA ORF clone, Ailuropoda melanoleuca(giant panda) -> XP_002914932.1 Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X17, mRNA.

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CloneID	OAc02553
Clone ID Related Accession (Same CDS sequence)	XM_002914886.3
Accession Version	XM_002914886.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-27
Organism	Ailuropoda melanoleuca(giant panda)
Product	dysferlin isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003217356.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 28, 2016 this sequence version replaced XM_002914886.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ailuropoda melanoleuca Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTATGCCGAG AATGTCCACA CCCCGGACAC CGACATCAGC 
GATGCCTACT GCTCTGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CAGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGAGCTCCG AGCTCCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
TTAGGGGAAG CCCAAGTCCC ACTCCGGGAG GTCCTCGCCA CCCCCAGTCT GTCTGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CGCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCCTCTGC ATCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATATG GTGGCTGACA CAGGAGGAGA GGAGGACACT 
GAGGACCAGG GGCTCACGGG AGATGAGGCA GAGCCATTAC TGGATCCGAG CGGTGCCCCA 
GGCCCTGGGG CCCCCACCAC CCCGAGGAAG CCACCTTCCC ATCCGCCCCC CTACCACCCC 
GGGAGCAAGA GAAAAAGAGG TGCTCCCACG CCCAGAAAGC TGCTGTCGGA CAAACCGCAG 
GACTTCCAGA TCAGGGTGCA AGTGATCGAG GGGCGCCAGC TGCCAGGGGT GAATATCAAG 
CCTGTGGTCA AAGTCACGGC TGCCGGGCAG ACCAAGAGGA CACGGATCCA CAAGGGAAAC 
AGCCCACTCT TCAACGAGAC TCTCTTCTTC AATGTGTTTG ACTCTCCCTC GGAGCTGTTT 
GACGAGCCCA TCTTTATCAC GGTGGTAGAC TCCCGTTCTC TCCGGACAGA CGCTCTCATT 
GGGGAATTCC GGATGGACGT GGGTACCATC TACAGAGAGC CCCGACACGC CTATCTGAGG 
AAATGGCTTC TCCTCTCGGA CCCTGACGAT TTCTCTGCTG GGGCCAGAGG TTACCTGAAA 
GCAAGCCTCT GTGTGCTGGG GCCCGGCGAT GAAGCGCCTC TGGAGAGAAA GGACCCCTCT 
GAAGACAAGG AGGACATCGA AAACAATCTG CTCAGGCCAA CTGGCGTGGC CCTTCGAGGA 
GCCCACTTCT GCCTGAAGGT CTTCAGAGCT GAGGACTTAC CACAGATGGA TGATGCCGTG 
ATGGACAACG TGAAGCAGAT CTTCGGCTTT GACAGTAACA AGAAGAACTT GGTGGATCCC 
TTCATGGAAG TCAGCTTTGC GGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAAACAGCC 
AACCCTCAGT GGAACCAGAG CATCACGCTG CCCGCCATGT TTCCCTCCAT GTGTGAAAAA 
ATGAGGATTC GCGTCATGGA CTGGGACCGC CTCACCCACA ATGACATCGT GGCCACCACC 
TACCTGAGTA TGTCGAAAAT CTCCGCCCCT GGAGGAGAAA TAGAAGTGGA CGACCATCTG 
GGCTTCCTCC CCACCTTCGG GCCCTGCTAC GTCAACCTCT ACGGCAGCCC CAGGGAGTTC 
ACCGGCTTCC CGGACCCCTA CGCAGAACTC AACATGGGCA AGGGAGAAGG TGTGGCCTAC 
CGAGGTCGGC TTCTGCTCTC CCTGGAGACC AAGCTGGTGG AGCACAGCGA CCAAAAGGTG 
GAGGACCTCC CTGCGGATGA CATCCTTCGG GTGGAGAAGT ATCTCCGGAG GCGTAAGTAC 
TCCCTCTTTG CCGCCTTCTA CTCGGCCTCC ATGCTCCAGG ATGTGGATGA TGCCATCCAG 
TTCGAGGTCA GCATCGGGAA CTATGGCAAC AAGTTCGACA CCACGTGCCT GCCGCTGGCC 
TCTACCACCC AGTACAGCCG GGCGGTCTTT GACGGGTGCC ACTACTACTA CTTGCCCTGG 
GGCAACGTGA AGCCCGTGGT GGTGCTGTCA TCCTACTGGG AGGACATCAG CCACAGAGTT 
GAGGCTCAGA ACCAGCTGCT CAGGATTGCG GACCGGCTGG AAGCTGCCCT GGAGCAAGTC 
CACTTGGCCG TGAAGGCGCA GTACAACGCC GAGGACGTGG ACTCCCTGTT GGCTCAGCTG 
ATGGATGAGC TCATCTCAGA CTGCAGCCAG CCCCTAGGCG ATGTCCACGA GACACCCTCT 
GCCACCCACC TGGACCAGTA CCTGCACAGG CTGCGTAGCC GTCACCTAAG CCAGATTGCA 
GAGGCGGCGC TGGCCCTGAA GCTCGGGCAC GGCGGGCTCC CGGCCGCCCT GGAGCAGGCT 
GAAGACTGGC TCCTGCGGCT CCGTGCCCTG GCCGAGGAGC CTCAGAACAG CCTGCCCGAC 
ATCATCATCT GGATGCTGCA GGGCGACAAG CGAGTGGCAT ACCAGCGGGT GCCTACACAT 
GAGGTCCTCT TCTCCCGGCG TGGAGCCAGC TACTGCGGCA AGAACTGTGG GAAGCTGCAG 
ACGATATTTC TCAAATATCC CATGCAGGGG GTGCCCGGAG CCCGGATGCC CGTGCAGATA 
CGGGTGAAGC TCTGGTTTGG GCTTTCTGTG GATGAGAAGG AGTTCAACCA GTTTGCTGAG 
GGAAAGCTGT CTGTCTTTGC TGAAACCTAT GAGAACCAGA CCAAACTGGC CCTCGTTGGG 
AACTGGGGCA CAACAGGCCT TACCTACCCC AAGTTTTCTG ATGTCGCGGG CAAGATCAAG 
CTCCCCAAGG ATAGCTTCCG CCCCTCAGCC GGCTGGACCT GGGCTGGAGA TTGGTTTGTG 
TGTCCAGAGA AGACGCTGCT CCATGACACC GACGCGGGCC ACCTGAGTTT CGTGGCGGAG 
GTGTTTGAGA ACCAGACCCG GCTCCCTGGA GGCCACTGGG TCTACATGAG TGACAACTAC 
ACGGATGTGA ATGGGGAGAA GGTGCTTCCC AAGGATGACA TTGAGTGCCC GCTGGGCTGG 
AAGTGGGAAG ATGAAGAGTG GTCCACGGAC CTCAATCGGG CCGTTGATGA GCAAGGCTGG 
GAGTACAGTA TCACCATCCC CCCGGACCGG AAGCCGAAGC ACTGGGTCCC CGCTGAGAAG 
ATGTACTACA CGCACCGTCG GCGGCGCTGG GTCCGCCTGC GCAGGAGGGA CCTCAGCCAG 
ATGGAGGCCC TGAGGAAGCA CAGGCAGGCG GAGGCGGAGG GCGAGGGCTG GGAGTACGCC 
TCGCTCTTTG GCTGGAAATT CCACCTGGAG TACCGCAAAA CGGATGCATT CCGTCGCCGC 
CGCTGGCGCC GCCGCATGGA GCCGCTGGAG AAGACGGGGC CTGCAGCCGT GTTTGCCCTT 
GAGGGGGCAC TGGGTGGCGT GGTAGATGAC CGGAGTGAAG ATTCCATGTC CGTGTCCACC 
TTGAGCTTTG GCGTGAACAG ACCCACGATT TCCTGCATCT TTGATCATGG GAACCGCTAC 
CACCTGCGCT GCTATATGTA CCAGGCTCGG GACCTGCCCT CGATGGACAA GGACTCTTTT 
TCTGATCCCT ACGCCATCGT CTCCTTCCTG CACCAGAGCC AGAAGACCGT GGTGGTGAAG 
AACACTCTGA ACCCCACCTG GGACCAGACG CTCATCTTCT ATGAGATTGA GATCTTTGGA 
GAGCCAGCCA GCATCGCCGA GCAGCCGCCC AGCATTGTGG TGGAGCTGTA TGACCACGAC 
ACCTACGGTG CAGATGAGTT TTTGGGACGC TGCATGTGTC AGCCCAGTCT GGAACGGATG 
CCCCGGCTGG CCTGGTTCCC GCTGGTTAGG GGCAGCCAGC CGGCAGGCGA GCTGCTGGCC 
TCTTTTGAGC TCATCCAGAG AGAGAAGCCG GCCATTCACC ATATTCCTGG TTTTGAGGTG 
CAGGATACAT CGGGAATCCT GGACGAGTCG GAGGACACAG ACCTGCCCTA CCCACCGCCT 
CAGAGGGAGG CCAACATCTA CATGGTTCCC CAGAACATCA AGCCGGTTCT CCAGCGCACT 
GCCATAGAGA TCCTGGCATG GGGCCTACGG AACATGAAGA GTTACCAGCT GGCCAGTATC 
TCCTCCCCCA GCCTCGTGGT GGAGTGCGGG GGCCAGACGG TGCAGTCCTG TGTCATCAGG 
AACCTCCGGA AGAACCCCAA CTTCGACATC TGCACCCTCT TCATGGAAGT GATGCTGCCC 
AGAGAGGAGC TCTACTGTCC CCCCATCGTG ATCAAGGTCA TTGATAACCG CCAGTTTGGC 
CGCCGGCCTG TGGTGGGCCA GTGTACCATA CGCTCCCTGG AGAGCTTCAT GTGTGACCTG 
TACTCCGAGG AGAGTCCGTC CCCACAGGGC GGCCCAGATG ATGTGAGTTT ACTCAGTCCT 
GGGGAAGACG TGCTCATTGA CATCGACGAC AAGGAGCCCC TCATCCCCAT ACAGCTTACA 
GACGGTCTGT TGGGCTCTGC CCCCACTAAC ATGGCATCCT CTCCATCCAG TCTTCATGAG 
GAGGAGTTCA TCGACTGGTG GAGCAAATTC TTTGCCTCCA CAGGGGAGAG GGAGAAGTGC 
GGCTCGTACT TGGAGAAGGG CTTTGACACT CTAAAGGTCT ATGACACACA ACTGGAGAAC 
GTGGAGGCCT TTGGGGGCCT GTCTGACTTT TGTAACACCT TCAAGCTCTA CCGGGGCAAG 
ACTCAGGAGG AGACGGAGGA CCCATCTGTT ATCGGGGAGT TTAAGGGCCT CTTCAAAATC 
TACCCCCTCC CGGAAGACCC GGCCGTCCCC ATGCCCCCGA GACAGTTCCA GCAGCTGGCT 
GCCCAGGGGC CTCAGGACTG CCTGGTCCGC ATCTATATCA TCCGGGCATT TGGCCTGCAG 
CCCAAGGACC CCAATGGGAA GTGTGATCCT TACATCAAGA TCTCCATAGG GAAGAAATCA 
GTGAGTGACC AGGATAATTA CATTCCCTGT ACCCTGGAGC CTGTGTTTGG AAAGATGTTC 
GAGCTGACCT GCACTCTGCC TCTGGAGAAG GACCTGAAGA TCACACTCTA CGACTATGAC 
CTCCTCTCCA AAGATGAGAA GATCGGGGAG ACAGTCATCG ACCTGGAGAA TAGGCTGCTG 
TCCAAGTTCG GGGCTCGCTG CGGACTCCCA CAGACCTACT GTGTCTCTGG ACCCAACCAG 
TGGAGGGACC AGCTCCGCCC CTCCCAGCTT CTCCAGCTCA CCTGCCAGCA GCGCAGACTC 
AAGGCGCCCG TGTACCTCAC AGACCGCGTA CTCTTTCAGG ATAAAGAATA CACCATTGAG 
GAAATAGAGG CTGGCAGGGT CCTGAACCCA CACCTGGGCC CAGTGGAAGA GCGTCTGGCC 
TTGCACGTCC TCCAGCAGCT GGGCCTGGTC CCCGAGCATG TGGAGTCCCG GCCTCTTTAC 
AGCCCCCTGC AGCCAGACAT TGAGCAGGGG AAGCTGCAGA TGTGGATCGA TCTGTTTCCC 
AAGGCCCTGG GGCGACCTGG ACCTCCCTTC AACATCACCC CACGGAGAGC AAGAAGGTTT 
TTCCTGCGTT GTATTATCTG GAACACAAAG GACGTGATCC TGGACGACCT CAGCATCACG 
GGGGAGAAGA TGAGTGACAT TTACGTGAAA GGTTGGATGG TCGGCTTTGA AGAGCACAAG 
CAGAAGACGG ACGTGCACTA CCGCTCCCTG GGAGGCGAAG GCAACTTTAA CTGGAGGTTC 
ATTTTCCCCT TTGACTACCT GCCCGCTGAG CAAGTCTGCA CCGTCTCTAA GAAGGATGCT 
TTCTGGAGGC TGGACAAGAC TGAGAGCAAA ATCCCAGCAC AAGTGGTATT TCAGATCTGG 
GACAATGACA AGTTCTCCTT TGACGACTTT CTGGGCTCGT TGCAGTTGGA TCTCAACCGC 
ATGCCCAAGC CTGCCAAGAC AGCTGAGAAG TGCTCCTTGG ACCAGCTGGA CGACACTTTC 
CACCCGGAGT GGTTTGTGTC CCTTTTTGAA CAGAAAACAG TAAAGGGCTG GTGGCCCTGT 
GTGGCAGAAG ACGGTGAGAA GAAGATCCTG GCGGGCAAGC TGGAAATGAC CTTGGAGATC 
GTAGCAGAGA GTGAGCACGA AGAGCGGCCT GCGGGTCAAG GCCGGGATGA ACCCAACATG 
AACCCCAAGC TCGAGGACCC AAGGCGCCCC GACACCTCCT TCCTGTGGTT CACCTCTCCG 
TACAAGACCA TGAAGTTCAT CCTGTGGCGG CGGTTCCGGT GCGCCATCAT CCTCTTCGTC 
GTTCTCGTCA TCCTGCTGCT CTTCCTGGGC ATCTTCCTCT ATTCCTTCCC GAACTATGCT 
GCCATGAAGC TGGTGAAACC CTTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002914932.1
CDS	487..6756
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEAQVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPSASLEPSPTLPDMDMVADTGGEEDTEDQGLTGDEAEPLLDPSGAPGPGAPTTPRKPPSHPPPYHPGSKRKRGAPTPRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVMDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDHLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRLLLSLETKLVEHSDQKVEDLPADDILRVEKYLRRRKYSLFAAFYSASMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADRLEAALEQVHLAVKAQYNAEDVDSLLAQLMDELISDCSQPLGDVHETPSATHLDQYLHRLRSRHLSQIAEAALALKLGHGGLPAALEQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPTHEVLFSRRGASYCGKNCGKLQTIFLKYPMQGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVAGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDTDAGHLSFVAEVFENQTRLPGGHWVYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALRKHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSMSVSTLSFGVNRPTISCIFDHGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFLGRCMCQPSLERMPRLAWFPLVRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTSGILDESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPIVIKVIDNRQFGRRPVVGQCTIRSLESFMCDLYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLTDGLLGSAPTNMASSPSSLHEEEFIDWWSKFFASTGEREKCGSYLEKGFDTLKVYDTQLENVEAFGGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAVPMPPRQFQQLAAQGPQDCLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLQLTCQQRRLKAPVYLTDRVLFQDKEYTIEEIEAGRVLNPHLGPVEERLALHVLQQLGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPAQVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEDGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFVVLVILLLFLGIFLYSFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_002914886.3
Organism	Ailuropoda melanoleuca(giant panda)
Definition	Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002914886.3

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCCGAG AATGTCCACA CCCCGGACAC CGACATCAGC 
GATGCCTACT GCTCTGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CAGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGAGCTCCG AGCTCCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
TTAGGGGAAG CCCAAGTCCC ACTCCGGGAG GTCCTCGCCA CCCCCAGTCT GTCTGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CGCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCCTCTGC ATCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATATG GTGGCTGACA CAGGAGGAGA GGAGGACACT 
GAGGACCAGG GGCTCACGGG AGATGAGGCA GAGCCATTAC TGGATCCGAG CGGTGCCCCA 
GGCCCTGGGG CCCCCACCAC CCCGAGGAAG CCACCTTCCC ATCCGCCCCC CTACCACCCC 
GGGAGCAAGA GAAAAAGAGG TGCTCCCACG CCCAGAAAGC TGCTGTCGGA CAAACCGCAG 
GACTTCCAGA TCAGGGTGCA AGTGATCGAG GGGCGCCAGC TGCCAGGGGT GAATATCAAG 
CCTGTGGTCA AAGTCACGGC TGCCGGGCAG ACCAAGAGGA CACGGATCCA CAAGGGAAAC 
AGCCCACTCT TCAACGAGAC TCTCTTCTTC AATGTGTTTG ACTCTCCCTC GGAGCTGTTT 
GACGAGCCCA TCTTTATCAC GGTGGTAGAC TCCCGTTCTC TCCGGACAGA CGCTCTCATT 
GGGGAATTCC GGATGGACGT GGGTACCATC TACAGAGAGC CCCGACACGC CTATCTGAGG 
AAATGGCTTC TCCTCTCGGA CCCTGACGAT TTCTCTGCTG GGGCCAGAGG TTACCTGAAA 
GCAAGCCTCT GTGTGCTGGG GCCCGGCGAT GAAGCGCCTC TGGAGAGAAA GGACCCCTCT 
GAAGACAAGG AGGACATCGA AAACAATCTG CTCAGGCCAA CTGGCGTGGC CCTTCGAGGA 
GCCCACTTCT GCCTGAAGGT CTTCAGAGCT GAGGACTTAC CACAGATGGA TGATGCCGTG 
ATGGACAACG TGAAGCAGAT CTTCGGCTTT GACAGTAACA AGAAGAACTT GGTGGATCCC 
TTCATGGAAG TCAGCTTTGC GGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAAACAGCC 
AACCCTCAGT GGAACCAGAG CATCACGCTG CCCGCCATGT TTCCCTCCAT GTGTGAAAAA 
ATGAGGATTC GCGTCATGGA CTGGGACCGC CTCACCCACA ATGACATCGT GGCCACCACC 
TACCTGAGTA TGTCGAAAAT CTCCGCCCCT GGAGGAGAAA TAGAAGTGGA CGACCATCTG 
GGCTTCCTCC CCACCTTCGG GCCCTGCTAC GTCAACCTCT ACGGCAGCCC CAGGGAGTTC 
ACCGGCTTCC CGGACCCCTA CGCAGAACTC AACATGGGCA AGGGAGAAGG TGTGGCCTAC 
CGAGGTCGGC TTCTGCTCTC CCTGGAGACC AAGCTGGTGG AGCACAGCGA CCAAAAGGTG 
GAGGACCTCC CTGCGGATGA CATCCTTCGG GTGGAGAAGT ATCTCCGGAG GCGTAAGTAC 
TCCCTCTTTG CCGCCTTCTA CTCGGCCTCC ATGCTCCAGG ATGTGGATGA TGCCATCCAG 
TTCGAGGTCA GCATCGGGAA CTATGGCAAC AAGTTCGACA CCACGTGCCT GCCGCTGGCC 
TCTACCACCC AGTACAGCCG GGCGGTCTTT GACGGGTGCC ACTACTACTA CTTGCCCTGG 
GGCAACGTGA AGCCCGTGGT GGTGCTGTCA TCCTACTGGG AGGACATCAG CCACAGAGTT 
GAGGCTCAGA ACCAGCTGCT CAGGATTGCG GACCGGCTGG AAGCTGCCCT GGAGCAAGTC 
CACTTGGCCG TGAAGGCGCA GTACAACGCC GAGGACGTGG ACTCCCTGTT GGCTCAGCTG 
ATGGATGAGC TCATCTCAGA CTGCAGCCAG CCCCTAGGCG ATGTCCACGA GACACCCTCT 
GCCACCCACC TGGACCAGTA CCTGCACAGG CTGCGTAGCC GTCACCTAAG CCAGATTGCA 
GAGGCGGCGC TGGCCCTGAA GCTCGGGCAC GGCGGGCTCC CGGCCGCCCT GGAGCAGGCT 
GAAGACTGGC TCCTGCGGCT CCGTGCCCTG GCCGAGGAGC CTCAGAACAG CCTGCCCGAC 
ATCATCATCT GGATGCTGCA GGGCGACAAG CGAGTGGCAT ACCAGCGGGT GCCTACACAT 
GAGGTCCTCT TCTCCCGGCG TGGAGCCAGC TACTGCGGCA AGAACTGTGG GAAGCTGCAG 
ACGATATTTC TCAAATATCC CATGCAGGGG GTGCCCGGAG CCCGGATGCC CGTGCAGATA 
CGGGTGAAGC TCTGGTTTGG GCTTTCTGTG GATGAGAAGG AGTTCAACCA GTTTGCTGAG 
GGAAAGCTGT CTGTCTTTGC TGAAACCTAT GAGAACCAGA CCAAACTGGC CCTCGTTGGG 
AACTGGGGCA CAACAGGCCT TACCTACCCC AAGTTTTCTG ATGTCGCGGG CAAGATCAAG 
CTCCCCAAGG ATAGCTTCCG CCCCTCAGCC GGCTGGACCT GGGCTGGAGA TTGGTTTGTG 
TGTCCAGAGA AGACGCTGCT CCATGACACC GACGCGGGCC ACCTGAGTTT CGTGGCGGAG 
GTGTTTGAGA ACCAGACCCG GCTCCCTGGA GGCCACTGGG TCTACATGAG TGACAACTAC 
ACGGATGTGA ATGGGGAGAA GGTGCTTCCC AAGGATGACA TTGAGTGCCC GCTGGGCTGG 
AAGTGGGAAG ATGAAGAGTG GTCCACGGAC CTCAATCGGG CCGTTGATGA GCAAGGCTGG 
GAGTACAGTA TCACCATCCC CCCGGACCGG AAGCCGAAGC ACTGGGTCCC CGCTGAGAAG 
ATGTACTACA CGCACCGTCG GCGGCGCTGG GTCCGCCTGC GCAGGAGGGA CCTCAGCCAG 
ATGGAGGCCC TGAGGAAGCA CAGGCAGGCG GAGGCGGAGG GCGAGGGCTG GGAGTACGCC 
TCGCTCTTTG GCTGGAAATT CCACCTGGAG TACCGCAAAA CGGATGCATT CCGTCGCCGC 
CGCTGGCGCC GCCGCATGGA GCCGCTGGAG AAGACGGGGC CTGCAGCCGT GTTTGCCCTT 
GAGGGGGCAC TGGGTGGCGT GGTAGATGAC CGGAGTGAAG ATTCCATGTC CGTGTCCACC 
TTGAGCTTTG GCGTGAACAG ACCCACGATT TCCTGCATCT TTGATCATGG GAACCGCTAC 
CACCTGCGCT GCTATATGTA CCAGGCTCGG GACCTGCCCT CGATGGACAA GGACTCTTTT 
TCTGATCCCT ACGCCATCGT CTCCTTCCTG CACCAGAGCC AGAAGACCGT GGTGGTGAAG 
AACACTCTGA ACCCCACCTG GGACCAGACG CTCATCTTCT ATGAGATTGA GATCTTTGGA 
GAGCCAGCCA GCATCGCCGA GCAGCCGCCC AGCATTGTGG TGGAGCTGTA TGACCACGAC 
ACCTACGGTG CAGATGAGTT TTTGGGACGC TGCATGTGTC AGCCCAGTCT GGAACGGATG 
CCCCGGCTGG CCTGGTTCCC GCTGGTTAGG GGCAGCCAGC CGGCAGGCGA GCTGCTGGCC 
TCTTTTGAGC TCATCCAGAG AGAGAAGCCG GCCATTCACC ATATTCCTGG TTTTGAGGTG 
CAGGATACAT CGGGAATCCT GGACGAGTCG GAGGACACAG ACCTGCCCTA CCCACCGCCT 
CAGAGGGAGG CCAACATCTA CATGGTTCCC CAGAACATCA AGCCGGTTCT CCAGCGCACT 
GCCATAGAGA TCCTGGCATG GGGCCTACGG AACATGAAGA GTTACCAGCT GGCCAGTATC 
TCCTCCCCCA GCCTCGTGGT GGAGTGCGGG GGCCAGACGG TGCAGTCCTG TGTCATCAGG 
AACCTCCGGA AGAACCCCAA CTTCGACATC TGCACCCTCT TCATGGAAGT GATGCTGCCC 
AGAGAGGAGC TCTACTGTCC CCCCATCGTG ATCAAGGTCA TTGATAACCG CCAGTTTGGC 
CGCCGGCCTG TGGTGGGCCA GTGTACCATA CGCTCCCTGG AGAGCTTCAT GTGTGACCTG 
TACTCCGAGG AGAGTCCGTC CCCACAGGGC GGCCCAGATG ATGTGAGTTT ACTCAGTCCT 
GGGGAAGACG TGCTCATTGA CATCGACGAC AAGGAGCCCC TCATCCCCAT ACAGCTTACA 
GACGGTCTGT TGGGCTCTGC CCCCACTAAC ATGGCATCCT CTCCATCCAG TCTTCATGAG 
GAGGAGTTCA TCGACTGGTG GAGCAAATTC TTTGCCTCCA CAGGGGAGAG GGAGAAGTGC 
GGCTCGTACT TGGAGAAGGG CTTTGACACT CTAAAGGTCT ATGACACACA ACTGGAGAAC 
GTGGAGGCCT TTGGGGGCCT GTCTGACTTT TGTAACACCT TCAAGCTCTA CCGGGGCAAG 
ACTCAGGAGG AGACGGAGGA CCCATCTGTT ATCGGGGAGT TTAAGGGCCT CTTCAAAATC 
TACCCCCTCC CGGAAGACCC GGCCGTCCCC ATGCCCCCGA GACAGTTCCA GCAGCTGGCT 
GCCCAGGGGC CTCAGGACTG CCTGGTCCGC ATCTATATCA TCCGGGCATT TGGCCTGCAG 
CCCAAGGACC CCAATGGGAA GTGTGATCCT TACATCAAGA TCTCCATAGG GAAGAAATCA 
GTGAGTGACC AGGATAATTA CATTCCCTGT ACCCTGGAGC CTGTGTTTGG AAAGATGTTC 
GAGCTGACCT GCACTCTGCC TCTGGAGAAG GACCTGAAGA TCACACTCTA CGACTATGAC 
CTCCTCTCCA AAGATGAGAA GATCGGGGAG ACAGTCATCG ACCTGGAGAA TAGGCTGCTG 
TCCAAGTTCG GGGCTCGCTG CGGACTCCCA CAGACCTACT GTGTCTCTGG ACCCAACCAG 
TGGAGGGACC AGCTCCGCCC CTCCCAGCTT CTCCAGCTCA CCTGCCAGCA GCGCAGACTC 
AAGGCGCCCG TGTACCTCAC AGACCGCGTA CTCTTTCAGG ATAAAGAATA CACCATTGAG 
GAAATAGAGG CTGGCAGGGT CCTGAACCCA CACCTGGGCC CAGTGGAAGA GCGTCTGGCC 
TTGCACGTCC TCCAGCAGCT GGGCCTGGTC CCCGAGCATG TGGAGTCCCG GCCTCTTTAC 
AGCCCCCTGC AGCCAGACAT TGAGCAGGGG AAGCTGCAGA TGTGGATCGA TCTGTTTCCC 
AAGGCCCTGG GGCGACCTGG ACCTCCCTTC AACATCACCC CACGGAGAGC AAGAAGGTTT 
TTCCTGCGTT GTATTATCTG GAACACAAAG GACGTGATCC TGGACGACCT CAGCATCACG 
GGGGAGAAGA TGAGTGACAT TTACGTGAAA GGTTGGATGG TCGGCTTTGA AGAGCACAAG 
CAGAAGACGG ACGTGCACTA CCGCTCCCTG GGAGGCGAAG GCAACTTTAA CTGGAGGTTC 
ATTTTCCCCT TTGACTACCT GCCCGCTGAG CAAGTCTGCA CCGTCTCTAA GAAGGATGCT 
TTCTGGAGGC TGGACAAGAC TGAGAGCAAA ATCCCAGCAC AAGTGGTATT TCAGATCTGG 
GACAATGACA AGTTCTCCTT TGACGACTTT CTGGGCTCGT TGCAGTTGGA TCTCAACCGC 
ATGCCCAAGC CTGCCAAGAC AGCTGAGAAG TGCTCCTTGG ACCAGCTGGA CGACACTTTC 
CACCCGGAGT GGTTTGTGTC CCTTTTTGAA CAGAAAACAG TAAAGGGCTG GTGGCCCTGT 
GTGGCAGAAG ACGGTGAGAA GAAGATCCTG GCGGGCAAGC TGGAAATGAC CTTGGAGATC 
GTAGCAGAGA GTGAGCACGA AGAGCGGCCT GCGGGTCAAG GCCGGGATGA ACCCAACATG 
AACCCCAAGC TCGAGGACCC AAGGCGCCCC GACACCTCCT TCCTGTGGTT CACCTCTCCG 
TACAAGACCA TGAAGTTCAT CCTGTGGCGG CGGTTCCGGT GCGCCATCAT CCTCTTCGTC 
GTTCTCGTCA TCCTGCTGCT CTTCCTGGGC ATCTTCCTCT ATTCCTTCCC GAACTATGCT 
GCCATGAAGC TGGTGAAACC CTTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_002914887.3 ) cDNA ORF clone, Ailuropoda melanoleuca(giant panda) -> XP_002914933.1 Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X21, mRNA.

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CloneID	OAc02554
Clone ID Related Accession (Same CDS sequence)	XM_002914887.3
Accession Version	XM_002914887.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-27
Organism	Ailuropoda melanoleuca(giant panda)
Product	dysferlin isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003217356.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 28, 2016 this sequence version replaced XM_002914887.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ailuropoda melanoleuca Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTATGCCGAG AATGTCCACA CCCCGGACAC CGACATCAGC 
GATGCCTACT GCTCTGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CAGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGAGCTCCG AGCTCCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
TTAGGGGAAG CCCAAGTCCC ACTCCGGGAG GTCCTCGCCA CCCCCAGTCT GTCTGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CGCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCCTCTGC ATCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATATG GTGGCTGACA CAGGAGGAGA GGAGGACACT 
GAGGACCAGG GGCTCACGGG AGATGAGGCA GAGCCATTAC TGGATCCGAG CGGTGCCCCA 
GGCCCTGGGG CCCCCACCAC CCCGAGGAAG CCACCTTCCC ATCCGCCCCC CTACCACCCC 
GGGAGCAAGA GAAAAAGAGG TGCTCCCACG CCCAGAAAGC TGCTGTCGGA CAAACCGCAG 
GACTTCCAGA TCAGGGTGCA AGTGATCGAG GGGCGCCAGC TGCCAGGGGT GAATATCAAG 
CCTGTGGTCA AAGTCACGGC TGCCGGGCAG ACCAAGAGGA CACGGATCCA CAAGGGAAAC 
AGCCCACTCT TCAACGAGAC TCTCTTCTTC AATGTGTTTG ACTCTCCCTC GGAGCTGTTT 
GACGAGCCCA TCTTTATCAC GGTGGTAGAC TCCCGTTCTC TCCGGACAGA CGCTCTCATT 
GGGGAATTCC GGATGGACGT GGGTACCATC TACAGAGAGC CCCGACACGC CTATCTGAGG 
AAATGGCTTC TCCTCTCGGA CCCTGACGAT TTCTCTGCTG GGGCCAGAGG TTACCTGAAA 
GCAAGCCTCT GTGTGCTGGG GCCCGGCGAT GAAGCGCCTC TGGAGAGAAA GGACCCCTCT 
GAAGACAAGG AGGACATCGA AAACAATCTG CTCAGGCCAA CTGGCGTGGC CCTTCGAGGA 
GCCCACTTCT GCCTGAAGGT CTTCAGAGCT GAGGACTTAC CACAGATGGA TGATGCCGTG 
ATGGACAACG TGAAGCAGAT CTTCGGCTTT GACAGTAACA AGAAGAACTT GGTGGATCCC 
TTCATGGAAG TCAGCTTTGC GGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAAACAGCC 
AACCCTCAGT GGAACCAGAG CATCACGCTG CCCGCCATGT TTCCCTCCAT GTGTGAAAAA 
ATGAGGATTC GCGTCATGGA CTGGGACCGC CTCACCCACA ATGACATCGT GGCCACCACC 
TACCTGAGTA TGTCGAAAAT CTCCGCCCCT GGAGGAGAAA TAGAAGTGGA CGACCATCTG 
GGCTTCCTCC CCACCTTCGG GCCCTGCTAC GTCAACCTCT ACGGCAGCCC CAGGGAGTTC 
ACCGGCTTCC CGGACCCCTA CGCAGAACTC AACATGGGCA AGGGAGAAGG TGTGGCCTAC 
CGAGGTCGGC TTCTGCTCTC CCTGGAGACC AAGCTGGTGG AGCACAGCGA CCAAAAGGTG 
GAGGACCTCC CTGCGGATGA CATCCTTCGG GTGGAGAAGT ATCTCCGGAG GCGTAAGTAC 
TCCCTCTTTG CCGCCTTCTA CTCGGCCTCC ATGCTCCAGG ATGTGGATGA TGCCATCCAG 
TTCGAGGTCA GCATCGGGAA CTATGGCAAC AAGTTCGACA CCACGTGCCT GCCGCTGGCC 
TCTACCACCC AGTACAGCCG GGCGGTCTTT GACGGGTGCC ACTACTACTA CTTGCCCTGG 
GGCAACGTGA AGCCCGTGGT GGTGCTGTCA TCCTACTGGG AGGACATCAG CCACAGAGTT 
GAGGCTCAGA ACCAGCTGCT CAGGATTGCG GACCGGCTGG AAGCTGCCCT GGAGCAAGTC 
CACTTGGCCG TGAAGGCGCA GTACAACGCC GAGGACGTGG ACTCCCTGTT GGCTCAGCTG 
ATGGATGAGC TCATCTCAGA CTGCAGCCAG CCCCTAGGCG ATGTCCACGA GACACCCTCT 
GCCACCCACC TGGACCAGTA CCTGCACAGG CTGCGTAGCC GTCACCTAAG CCAGATTGCA 
GAGGCGGCGC TGGCCCTGAA GCTCGGGCAC GGCGGGCTCC CGGCCGCCCT GGAGCAGGCT 
GAAGACTGGC TCCTGCGGCT CCGTGCCCTG GCCGAGGAGC CTCAGAACAG CCTGCCCGAC 
ATCATCATCT GGATGCTGCA GGGCGACAAG CGAGTGGCAT ACCAGCGGGT GCCTACACAT 
GAGGTCCTCT TCTCCCGGCG TGGAGCCAGC TACTGCGGCA AGAACTGTGG GAAGCTGCAG 
ACGATATTTC TCAAATATCC CATGCAGGGG GTGCCCGGAG CCCGGATGCC CGTGCAGATA 
CGGGTGAAGC TCTGGTTTGG GCTTTCTGTG GATGAGAAGG AGTTCAACCA GTTTGCTGAG 
GGAAAGCTGT CTGTCTTTGC TGAAACCTAT GAGAACCAGA CCAAACTGGC CCTCGTTGGG 
AACTGGGGCA CAACAGGCCT TACCTACCCC AAGTTTTCTG ATGTCGCGGG CAAGATCAAG 
CTCCCCAAGG ATAGCTTCCG CCCCTCAGCC GGCTGGACCT GGGCTGGAGA TTGGTTTGTG 
TGTCCAGAGA AGACGCTGCT CCATGACACC GACGCGGGCC ACCTGAGTTT CGTGGCGGAG 
GTGTTTGAGA ACCAGACCCG GCTCCCTGGA GGCCACTGGG TCTACATGAG TGACAACTAC 
ACGGATGTGA ATGGGGAGAA GGTGCTTCCC AAGGATGACA TTGAGTGCCC GCTGGGCTGG 
AAGTGGGAAG ATGAAGAGTG GTCCACGGAC CTCAATCGGG CCGTTGATGA GCAAGGCTGG 
GAGTACAGTA TCACCATCCC CCCGGACCGG AAGCCGAAGC ACTGGGTCCC CGCTGAGAAG 
ATGTACTACA CGCACCGTCG GCGGCGCTGG GTCCGCCTGC GCAGGAGGGA CCTCAGCCAG 
ATGGAGGCCC TGAGGAAGCA CAGGCAGGCG GAGGCGGAGG GCGAGGGCTG GGAGTACGCC 
TCGCTCTTTG GCTGGAAATT CCACCTGGAG TACCGCAAAA CGGATGCATT CCGTCGCCGC 
CGCTGGCGCC GCCGCATGGA GCCGCTGGAG AAGACGGGGC CTGCAGCCGT GTTTGCCCTT 
GAGGGGGCAC TGGGTGGCGT GGTAGATGAC CGGAGTGAAG ATTCCATGTC CGTGTCCACC 
TTGAGCTTTG GCGTGAACAG ACCCACGATT TCCTGCATCT TTGATCATGG GAACCGCTAC 
CACCTGCGCT GCTATATGTA CCAGGCTCGG GACCTGCCCT CGATGGACAA GGACTCTTTT 
TCTGATCCCT ACGCCATCGT CTCCTTCCTG CACCAGAGCC AGAAGACCGT GGTGGTGAAG 
AACACTCTGA ACCCCACCTG GGACCAGACG CTCATCTTCT ATGAGATTGA GATCTTTGGA 
GAGCCAGCCA GCATCGCCGA GCAGCCGCCC AGCATTGTGG TGGAGCTGTA TGACCACGAC 
ACCTACGGTG CAGATGAGTT TTTGGGACGC TGCATGTGTC AGCCCAGTCT GGAACGGATG 
CCCCGGCTGG CCTGGTTCCC GCTGGTTAGG GGCAGCCAGC CGGCAGGCGA GCTGCTGGCC 
TCTTTTGAGC TCATCCAGAG AGAGAAGCCG GCCATTCACC ATATTCCTGG TTTTGAGGTG 
CAGGATACAT CGGGAATCCT GGACGAGTCG GAGGACACAG ACCTGCCCTA CCCACCGCCT 
CAGAGGGAGG CCAACATCTA CATGGTTCCC CAGAACATCA AGCCGGTTCT CCAGCGCACT 
GCCATAGAGA TCCTGGCATG GGGCCTACGG AACATGAAGA GTTACCAGCT GGCCAGTATC 
TCCTCCCCCA GCCTCGTGGT GGAGTGCGGG GGCCAGACGG TGCAGTCCTG TGTCATCAGG 
AACCTCCGGA AGAACCCCAA CTTCGACATC TGCACCCTCT TCATGGAAGT GATGCTGCCC 
AGAGAGGAGC TCTACTGTCC CCCCATCGTG ATCAAGGTCA TTGATAACCG CCAGTTTGGC 
CGCCGGCCTG TGGTGGGCCA GTGTACCATA CGCTCCCTGG AGAGCTTCAT GTGTGACCTG 
TACTCCGAGG AGAGTCCGTC CCCACAGGGC GGCCCAGATG ATGTGAGTTT ACTCAGTCCT 
GGGGAAGACG TGCTCATTGA CATCGACGAC AAGGAGCCCC TCATCCCCAT ACAGGAGGAG 
GAGTTCATCG ACTGGTGGAG CAAATTCTTT GCCTCCACAG GGGAGAGGGA GAAGTGCGGC 
TCGTACTTGG AGAAGGGCTT TGACACTCTA AAGGTCTATG ACACACAACT GGAGAACGTG 
GAGGCCTTTG GGGGCCTGTC TGACTTTTGT AACACCTTCA AGCTCTACCG GGGCAAGACT 
CAGGAGGAGA CGGAGGACCC ATCTGTTATC GGGGAGTTTA AGGGCCTCTT CAAAATCTAC 
CCCCTCCCGG AAGACCCGGC CGTCCCCATG CCCCCGAGAC AGTTCCAGCA GCTGGCTGCC 
CAGGGGCCTC AGGACTGCCT GGTCCGCATC TATATCATCC GGGCATTTGG CCTGCAGCCC 
AAGGACCCCA ATGGGAAGTG TGATCCTTAC ATCAAGATCT CCATAGGGAA GAAATCAGTG 
AGTGACCAGG ATAATTACAT TCCCTGTACC CTGGAGCCTG TGTTTGGAAA GATGTTCGAG 
CTGACCTGCA CTCTGCCTCT GGAGAAGGAC CTGAAGATCA CACTCTACGA CTATGACCTC 
CTCTCCAAAG ATGAGAAGAT CGGGGAGACA GTCATCGACC TGGAGAATAG GCTGCTGTCC 
AAGTTCGGGG CTCGCTGCGG ACTCCCACAG ACCTACTGTG TCTCTGGACC CAACCAGTGG 
AGGGACCAGC TCCGCCCCTC CCAGCTTCTC CAGCTCACCT GCCAGCAGCG CAGACTCAAG 
GCGCCCGTGT ACCTCACAGA CCGCGTACTC TTTCAGGATA AAGAATACAC CATTGAGGAA 
ATAGAGGCTG GCAGGGTCCT GAACCCACAC CTGGGCCCAG TGGAAGAGCG TCTGGCCTTG 
CACGTCCTCC AGCAGCTGGG CCTGGTCCCC GAGCATGTGG AGTCCCGGCC TCTTTACAGC 
CCCCTGCAGC CAGACATTGA GCAGGGGAAG CTGCAGATGT GGATCGATCT GTTTCCCAAG 
GCCCTGGGGC GACCTGGACC TCCCTTCAAC ATCACCCCAC GGAGAGCAAG AAGGTTTTTC 
CTGCGTTGTA TTATCTGGAA CACAAAGGAC GTGATCCTGG ACGACCTCAG CATCACGGGG 
GAGAAGATGA GTGACATTTA CGTGAAAGGT TGGATGGTCG GCTTTGAAGA GCACAAGCAG 
AAGACGGACG TGCACTACCG CTCCCTGGGA GGCGAAGGCA ACTTTAACTG GAGGTTCATT 
TTCCCCTTTG ACTACCTGCC CGCTGAGCAA GTCTGCACCG TCTCTAAGAA GGATGCTTTC 
TGGAGGCTGG ACAAGACTGA GAGCAAAATC CCAGCACAAG TGGTATTTCA GATCTGGGAC 
AATGACAAGT TCTCCTTTGA CGACTTTCTG GGCTCGTTGC AGTTGGATCT CAACCGCATG 
CCCAAGCCTG CCAAGACAGC TGAGAAGTGC TCCTTGGACC AGCTGGACGA CACTTTCCAC 
CCGGAGTGGT TTGTGTCCCT TTTTGAACAG AAAACAGTAA AGGGCTGGTG GCCCTGTGTG 
GCAGAAGACG GTGAGAAGAA GATCCTGGCG GGCAAGCTGG AAATGACCTT GGAGATCGTA 
GCAGAGAGTG AGCACGAAGA GCGGCCTGCG GGTCAAGGCC GGGATGAACC CAACATGAAC 
CCCAAGCTCG AGGACCCAAG GCGCCCCGAC ACCTCCTTCC TGTGGTTCAC CTCTCCGTAC 
AAGACCATGA AGTTCATCCT GTGGCGGCGG TTCCGGTGCG CCATCATCCT CTTCGTCGTT 
CTCGTCATCC TGCTGCTCTT CCTGGGCATC TTCCTCTATT CCTTCCCGAA CTATGCTGCC 
ATGAAGCTGG TGAAACCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002914933.1
CDS	487..6693
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEAQVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPSASLEPSPTLPDMDMVADTGGEEDTEDQGLTGDEAEPLLDPSGAPGPGAPTTPRKPPSHPPPYHPGSKRKRGAPTPRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVMDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDHLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRLLLSLETKLVEHSDQKVEDLPADDILRVEKYLRRRKYSLFAAFYSASMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADRLEAALEQVHLAVKAQYNAEDVDSLLAQLMDELISDCSQPLGDVHETPSATHLDQYLHRLRSRHLSQIAEAALALKLGHGGLPAALEQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPTHEVLFSRRGASYCGKNCGKLQTIFLKYPMQGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVAGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDTDAGHLSFVAEVFENQTRLPGGHWVYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALRKHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSMSVSTLSFGVNRPTISCIFDHGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFLGRCMCQPSLERMPRLAWFPLVRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTSGILDESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPIVIKVIDNRQFGRRPVVGQCTIRSLESFMCDLYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASTGEREKCGSYLEKGFDTLKVYDTQLENVEAFGGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAVPMPPRQFQQLAAQGPQDCLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLQLTCQQRRLKAPVYLTDRVLFQDKEYTIEEIEAGRVLNPHLGPVEERLALHVLQQLGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPAQVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEDGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFVVLVILLLFLGIFLYSFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_002914887.3
Organism	Ailuropoda melanoleuca(giant panda)
Definition	Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002914887.3

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCCGAG AATGTCCACA CCCCGGACAC CGACATCAGC 
GATGCCTACT GCTCTGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CAGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGAGCTCCG AGCTCCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
TTAGGGGAAG CCCAAGTCCC ACTCCGGGAG GTCCTCGCCA CCCCCAGTCT GTCTGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CGCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCCTCTGC ATCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATATG GTGGCTGACA CAGGAGGAGA GGAGGACACT 
GAGGACCAGG GGCTCACGGG AGATGAGGCA GAGCCATTAC TGGATCCGAG CGGTGCCCCA 
GGCCCTGGGG CCCCCACCAC CCCGAGGAAG CCACCTTCCC ATCCGCCCCC CTACCACCCC 
GGGAGCAAGA GAAAAAGAGG TGCTCCCACG CCCAGAAAGC TGCTGTCGGA CAAACCGCAG 
GACTTCCAGA TCAGGGTGCA AGTGATCGAG GGGCGCCAGC TGCCAGGGGT GAATATCAAG 
CCTGTGGTCA AAGTCACGGC TGCCGGGCAG ACCAAGAGGA CACGGATCCA CAAGGGAAAC 
AGCCCACTCT TCAACGAGAC TCTCTTCTTC AATGTGTTTG ACTCTCCCTC GGAGCTGTTT 
GACGAGCCCA TCTTTATCAC GGTGGTAGAC TCCCGTTCTC TCCGGACAGA CGCTCTCATT 
GGGGAATTCC GGATGGACGT GGGTACCATC TACAGAGAGC CCCGACACGC CTATCTGAGG 
AAATGGCTTC TCCTCTCGGA CCCTGACGAT TTCTCTGCTG GGGCCAGAGG TTACCTGAAA 
GCAAGCCTCT GTGTGCTGGG GCCCGGCGAT GAAGCGCCTC TGGAGAGAAA GGACCCCTCT 
GAAGACAAGG AGGACATCGA AAACAATCTG CTCAGGCCAA CTGGCGTGGC CCTTCGAGGA 
GCCCACTTCT GCCTGAAGGT CTTCAGAGCT GAGGACTTAC CACAGATGGA TGATGCCGTG 
ATGGACAACG TGAAGCAGAT CTTCGGCTTT GACAGTAACA AGAAGAACTT GGTGGATCCC 
TTCATGGAAG TCAGCTTTGC GGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAAACAGCC 
AACCCTCAGT GGAACCAGAG CATCACGCTG CCCGCCATGT TTCCCTCCAT GTGTGAAAAA 
ATGAGGATTC GCGTCATGGA CTGGGACCGC CTCACCCACA ATGACATCGT GGCCACCACC 
TACCTGAGTA TGTCGAAAAT CTCCGCCCCT GGAGGAGAAA TAGAAGTGGA CGACCATCTG 
GGCTTCCTCC CCACCTTCGG GCCCTGCTAC GTCAACCTCT ACGGCAGCCC CAGGGAGTTC 
ACCGGCTTCC CGGACCCCTA CGCAGAACTC AACATGGGCA AGGGAGAAGG TGTGGCCTAC 
CGAGGTCGGC TTCTGCTCTC CCTGGAGACC AAGCTGGTGG AGCACAGCGA CCAAAAGGTG 
GAGGACCTCC CTGCGGATGA CATCCTTCGG GTGGAGAAGT ATCTCCGGAG GCGTAAGTAC 
TCCCTCTTTG CCGCCTTCTA CTCGGCCTCC ATGCTCCAGG ATGTGGATGA TGCCATCCAG 
TTCGAGGTCA GCATCGGGAA CTATGGCAAC AAGTTCGACA CCACGTGCCT GCCGCTGGCC 
TCTACCACCC AGTACAGCCG GGCGGTCTTT GACGGGTGCC ACTACTACTA CTTGCCCTGG 
GGCAACGTGA AGCCCGTGGT GGTGCTGTCA TCCTACTGGG AGGACATCAG CCACAGAGTT 
GAGGCTCAGA ACCAGCTGCT CAGGATTGCG GACCGGCTGG AAGCTGCCCT GGAGCAAGTC 
CACTTGGCCG TGAAGGCGCA GTACAACGCC GAGGACGTGG ACTCCCTGTT GGCTCAGCTG 
ATGGATGAGC TCATCTCAGA CTGCAGCCAG CCCCTAGGCG ATGTCCACGA GACACCCTCT 
GCCACCCACC TGGACCAGTA CCTGCACAGG CTGCGTAGCC GTCACCTAAG CCAGATTGCA 
GAGGCGGCGC TGGCCCTGAA GCTCGGGCAC GGCGGGCTCC CGGCCGCCCT GGAGCAGGCT 
GAAGACTGGC TCCTGCGGCT CCGTGCCCTG GCCGAGGAGC CTCAGAACAG CCTGCCCGAC 
ATCATCATCT GGATGCTGCA GGGCGACAAG CGAGTGGCAT ACCAGCGGGT GCCTACACAT 
GAGGTCCTCT TCTCCCGGCG TGGAGCCAGC TACTGCGGCA AGAACTGTGG GAAGCTGCAG 
ACGATATTTC TCAAATATCC CATGCAGGGG GTGCCCGGAG CCCGGATGCC CGTGCAGATA 
CGGGTGAAGC TCTGGTTTGG GCTTTCTGTG GATGAGAAGG AGTTCAACCA GTTTGCTGAG 
GGAAAGCTGT CTGTCTTTGC TGAAACCTAT GAGAACCAGA CCAAACTGGC CCTCGTTGGG 
AACTGGGGCA CAACAGGCCT TACCTACCCC AAGTTTTCTG ATGTCGCGGG CAAGATCAAG 
CTCCCCAAGG ATAGCTTCCG CCCCTCAGCC GGCTGGACCT GGGCTGGAGA TTGGTTTGTG 
TGTCCAGAGA AGACGCTGCT CCATGACACC GACGCGGGCC ACCTGAGTTT CGTGGCGGAG 
GTGTTTGAGA ACCAGACCCG GCTCCCTGGA GGCCACTGGG TCTACATGAG TGACAACTAC 
ACGGATGTGA ATGGGGAGAA GGTGCTTCCC AAGGATGACA TTGAGTGCCC GCTGGGCTGG 
AAGTGGGAAG ATGAAGAGTG GTCCACGGAC CTCAATCGGG CCGTTGATGA GCAAGGCTGG 
GAGTACAGTA TCACCATCCC CCCGGACCGG AAGCCGAAGC ACTGGGTCCC CGCTGAGAAG 
ATGTACTACA CGCACCGTCG GCGGCGCTGG GTCCGCCTGC GCAGGAGGGA CCTCAGCCAG 
ATGGAGGCCC TGAGGAAGCA CAGGCAGGCG GAGGCGGAGG GCGAGGGCTG GGAGTACGCC 
TCGCTCTTTG GCTGGAAATT CCACCTGGAG TACCGCAAAA CGGATGCATT CCGTCGCCGC 
CGCTGGCGCC GCCGCATGGA GCCGCTGGAG AAGACGGGGC CTGCAGCCGT GTTTGCCCTT 
GAGGGGGCAC TGGGTGGCGT GGTAGATGAC CGGAGTGAAG ATTCCATGTC CGTGTCCACC 
TTGAGCTTTG GCGTGAACAG ACCCACGATT TCCTGCATCT TTGATCATGG GAACCGCTAC 
CACCTGCGCT GCTATATGTA CCAGGCTCGG GACCTGCCCT CGATGGACAA GGACTCTTTT 
TCTGATCCCT ACGCCATCGT CTCCTTCCTG CACCAGAGCC AGAAGACCGT GGTGGTGAAG 
AACACTCTGA ACCCCACCTG GGACCAGACG CTCATCTTCT ATGAGATTGA GATCTTTGGA 
GAGCCAGCCA GCATCGCCGA GCAGCCGCCC AGCATTGTGG TGGAGCTGTA TGACCACGAC 
ACCTACGGTG CAGATGAGTT TTTGGGACGC TGCATGTGTC AGCCCAGTCT GGAACGGATG 
CCCCGGCTGG CCTGGTTCCC GCTGGTTAGG GGCAGCCAGC CGGCAGGCGA GCTGCTGGCC 
TCTTTTGAGC TCATCCAGAG AGAGAAGCCG GCCATTCACC ATATTCCTGG TTTTGAGGTG 
CAGGATACAT CGGGAATCCT GGACGAGTCG GAGGACACAG ACCTGCCCTA CCCACCGCCT 
CAGAGGGAGG CCAACATCTA CATGGTTCCC CAGAACATCA AGCCGGTTCT CCAGCGCACT 
GCCATAGAGA TCCTGGCATG GGGCCTACGG AACATGAAGA GTTACCAGCT GGCCAGTATC 
TCCTCCCCCA GCCTCGTGGT GGAGTGCGGG GGCCAGACGG TGCAGTCCTG TGTCATCAGG 
AACCTCCGGA AGAACCCCAA CTTCGACATC TGCACCCTCT TCATGGAAGT GATGCTGCCC 
AGAGAGGAGC TCTACTGTCC CCCCATCGTG ATCAAGGTCA TTGATAACCG CCAGTTTGGC 
CGCCGGCCTG TGGTGGGCCA GTGTACCATA CGCTCCCTGG AGAGCTTCAT GTGTGACCTG 
TACTCCGAGG AGAGTCCGTC CCCACAGGGC GGCCCAGATG ATGTGAGTTT ACTCAGTCCT 
GGGGAAGACG TGCTCATTGA CATCGACGAC AAGGAGCCCC TCATCCCCAT ACAGGAGGAG 
GAGTTCATCG ACTGGTGGAG CAAATTCTTT GCCTCCACAG GGGAGAGGGA GAAGTGCGGC 
TCGTACTTGG AGAAGGGCTT TGACACTCTA AAGGTCTATG ACACACAACT GGAGAACGTG 
GAGGCCTTTG GGGGCCTGTC TGACTTTTGT AACACCTTCA AGCTCTACCG GGGCAAGACT 
CAGGAGGAGA CGGAGGACCC ATCTGTTATC GGGGAGTTTA AGGGCCTCTT CAAAATCTAC 
CCCCTCCCGG AAGACCCGGC CGTCCCCATG CCCCCGAGAC AGTTCCAGCA GCTGGCTGCC 
CAGGGGCCTC AGGACTGCCT GGTCCGCATC TATATCATCC GGGCATTTGG CCTGCAGCCC 
AAGGACCCCA ATGGGAAGTG TGATCCTTAC ATCAAGATCT CCATAGGGAA GAAATCAGTG 
AGTGACCAGG ATAATTACAT TCCCTGTACC CTGGAGCCTG TGTTTGGAAA GATGTTCGAG 
CTGACCTGCA CTCTGCCTCT GGAGAAGGAC CTGAAGATCA CACTCTACGA CTATGACCTC 
CTCTCCAAAG ATGAGAAGAT CGGGGAGACA GTCATCGACC TGGAGAATAG GCTGCTGTCC 
AAGTTCGGGG CTCGCTGCGG ACTCCCACAG ACCTACTGTG TCTCTGGACC CAACCAGTGG 
AGGGACCAGC TCCGCCCCTC CCAGCTTCTC CAGCTCACCT GCCAGCAGCG CAGACTCAAG 
GCGCCCGTGT ACCTCACAGA CCGCGTACTC TTTCAGGATA AAGAATACAC CATTGAGGAA 
ATAGAGGCTG GCAGGGTCCT GAACCCACAC CTGGGCCCAG TGGAAGAGCG TCTGGCCTTG 
CACGTCCTCC AGCAGCTGGG CCTGGTCCCC GAGCATGTGG AGTCCCGGCC TCTTTACAGC 
CCCCTGCAGC CAGACATTGA GCAGGGGAAG CTGCAGATGT GGATCGATCT GTTTCCCAAG 
GCCCTGGGGC GACCTGGACC TCCCTTCAAC ATCACCCCAC GGAGAGCAAG AAGGTTTTTC 
CTGCGTTGTA TTATCTGGAA CACAAAGGAC GTGATCCTGG ACGACCTCAG CATCACGGGG 
GAGAAGATGA GTGACATTTA CGTGAAAGGT TGGATGGTCG GCTTTGAAGA GCACAAGCAG 
AAGACGGACG TGCACTACCG CTCCCTGGGA GGCGAAGGCA ACTTTAACTG GAGGTTCATT 
TTCCCCTTTG ACTACCTGCC CGCTGAGCAA GTCTGCACCG TCTCTAAGAA GGATGCTTTC 
TGGAGGCTGG ACAAGACTGA GAGCAAAATC CCAGCACAAG TGGTATTTCA GATCTGGGAC 
AATGACAAGT TCTCCTTTGA CGACTTTCTG GGCTCGTTGC AGTTGGATCT CAACCGCATG 
CCCAAGCCTG CCAAGACAGC TGAGAAGTGC TCCTTGGACC AGCTGGACGA CACTTTCCAC 
CCGGAGTGGT TTGTGTCCCT TTTTGAACAG AAAACAGTAA AGGGCTGGTG GCCCTGTGTG 
GCAGAAGACG GTGAGAAGAA GATCCTGGCG GGCAAGCTGG AAATGACCTT GGAGATCGTA 
GCAGAGAGTG AGCACGAAGA GCGGCCTGCG GGTCAAGGCC GGGATGAACC CAACATGAAC 
CCCAAGCTCG AGGACCCAAG GCGCCCCGAC ACCTCCTTCC TGTGGTTCAC CTCTCCGTAC 
AAGACCATGA AGTTCATCCT GTGGCGGCGG TTCCGGTGCG CCATCATCCT CTTCGTCGTT 
CTCGTCATCC TGCTGCTCTT CCTGGGCATC TTCCTCTATT CCTTCCCGAA CTATGCTGCC 
ATGAAGCTGG TGAAACCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_002914890.3 ) cDNA ORF clone, Ailuropoda melanoleuca(giant panda) -> XP_002914936.1 Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X6, mRNA.

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CloneID	OAc02557
Clone ID Related Accession (Same CDS sequence)	XM_002914890.3
Accession Version	XM_002914890.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6366bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-27
Organism	Ailuropoda melanoleuca(giant panda)
Product	dysferlin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003217356.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 28, 2016 this sequence version replaced XM_002914890.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ailuropoda melanoleuca Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGTGAAGAA GGACCGGCGC 
AGTGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCAGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTCCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCGG GAGGTCCTCG CCACCCCCAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCCTC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGTGGCTG GTGGGGGACA GAGCCGGGCT 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACGGGAGAT 
GAGGCAGAGC CATTACTGGA TCCGAGCGGT GCCCCAGGCC CTGGGGCCCC CACCACCCCG 
AGGAAGCCAC CTTCCCATCC GCCCCCCTAC CACCCCGGGA GCAAGAGAAA AAGAGGTGCT 
CCCACGCCCA GAAAGCTGCT GTCGGACAAA CCGCAGGACT TCCAGATCAG GGTGCAAGTG 
ATCGAGGGGC GCCAGCTGCC AGGGGTGAAT ATCAAGCCTG TGGTCAAAGT CACGGCTGCC 
GGGCAGACCA AGAGGACACG GATCCACAAG GGAAACAGCC CACTCTTCAA CGAGACTCTC 
TTCTTCAATG TGTTTGACTC TCCCTCGGAG CTGTTTGACG AGCCCATCTT TATCACGGTG 
GTAGACTCCC GTTCTCTCCG GACAGACGCT CTCATTGGGG AATTCCGGAT GGACGTGGGT 
ACCATCTACA GAGAGCCCCG ACACGCCTAT CTGAGGAAAT GGCTTCTCCT CTCGGACCCT 
GACGATTTCT CTGCTGGGGC CAGAGGTTAC CTGAAAGCAA GCCTCTGTGT GCTGGGGCCC 
GGCGATGAAG CGCCTCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATCGAAAAC 
AATCTGCTCA GGCCAACTGG CGTGGCCCTT CGAGGAGCCC ACTTCTGCCT GAAGGTCTTC 
AGAGCTGAGG ACTTACCACA GATGGATGAT GCCGTGATGG ACAACGTGAA GCAGATCTTC 
GGCTTTGACA GTAACAAGAA GAACTTGGTG GATCCCTTCA TGGAAGTCAG CTTTGCGGGG 
AAAATGCTCT GCAGCAAGAT CCTGGAGAAA ACAGCCAACC CTCAGTGGAA CCAGAGCATC 
ACGCTGCCCG CCATGTTTCC CTCCATGTGT GAAAAAATGA GGATTCGCGT CATGGACTGG 
GACCGCCTCA CCCACAATGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCC 
GCCCCTGGAG GAGAAATAGA AGTGGACGAC CATCTGGGCT TCCTCCCCAC CTTCGGGCCC 
TGCTACGTCA ACCTCTACGG CAGCCCCAGG GAGTTCACCG GCTTCCCGGA CCCCTACGCA 
GAACTCAACA TGGGCAAGGG AGAAGGTGTG GCCTACCGAG GTCGGCTTCT GCTCTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGCGACCAA AAGGTGGAGG ACCTCCCTGC GGATGACATC 
CTTCGGGTGG AGAAGTATCT CCGGAGGCGT AAGTACTCCC TCTTTGCCGC CTTCTACTCG 
GCCTCCATGC TCCAGGATGT GGATGATGCC ATCCAGTTCG AGGTCAGCAT CGGGAACTAT 
GGCAACAAGT TCGACACCAC GTGCCTGCCG CTGGCCTCTA CCACCCAGTA CAGCCGGGCG 
GTCTTTGACG GGTGCCACTA CTACTACTTG CCCTGGGGCA ACGTGAAGCC CGTGGTGGTG 
CTGTCATCCT ACTGGGAGGA CATCAGCCAC AGAGTTGAGG CTCAGAACCA GCTGCTCAGG 
ATTGCGGACC GGCTGGAAGC TGCCCTGGAG CAAGTCCACT TGGCCGTGAA GGCGCAGTAC 
AACGCCGAGG ACGTGGACTC CCTGTTGGCT CAGCTGATGG ATGAGCTCAT CTCAGACTGC 
AGCCAGCCCC TAGGCGATGT CCACGAGACA CCCTCTGCCA CCCACCTGGA CCAGTACCTG 
CACAGGCTGC GTAGCCGTCA CCTAAGCCAG ATTGCAGAGG CGGCGCTGGC CCTGAAGCTC 
GGGCACGGCG GGCTCCCGGC CGCCCTGGAG CAGGCTGAAG ACTGGCTCCT GCGGCTCCGT 
GCCCTGGCCG AGGAGCCTCA GAACAGCCTG CCCGACATCA TCATCTGGAT GCTGCAGGGC 
GACAAGCGAG TGGCATACCA GCGGGTGCCT ACACATGAGG TCCTCTTCTC CCGGCGTGGA 
GCCAGCTACT GCGGCAAGAA CTGTGGGAAG CTGCAGACGA TATTTCTCAA ATATCCCATG 
CAGGGGGTGC CCGGAGCCCG GATGCCCGTG CAGATACGGG TGAAGCTCTG GTTTGGGCTT 
TCTGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGAA AGCTGTCTGT CTTTGCTGAA 
ACCTATGAGA ACCAGACCAA ACTGGCCCTC GTTGGGAACT GGGGCACAAC AGGCCTTACC 
TACCCCAAGT TTTCTGATGT CGCGGGCAAG ATCAAGCTCC CCAAGGATAG CTTCCGCCCC 
TCAGCCGGCT GGACCTGGGC TGGAGATTGG TTTGTGTGTC CAGAGAAGAC GCTGCTCCAT 
GACACCGACG CGGGCCACCT GAGTTTCGTG GCGGAGGTGT TTGAGAACCA GACCCGGCTC 
CCTGGAGGCC ACTGGGTCTA CATGAGTGAC AACTACACGG ATGTGAATGG GGAGAAGGTG 
CTTCCCAAGG ATGACATTGA GTGCCCGCTG GGCTGGAAGT GGGAAGATGA AGAGTGGTCC 
ACGGACCTCA ATCGGGCCGT TGATGAGCAA GGCTGGGAGT ACAGTATCAC CATCCCCCCG 
GACCGGAAGC CGAAGCACTG GGTCCCCGCT GAGAAGATGT ACTACACGCA CCGTCGGCGG 
CGCTGGGTCC GCCTGCGCAG GAGGGACCTC AGCCAGATGG AGGCCCTGAG GAAGCACAGG 
CAGGCGGAGG CGGAGGGCGA GGGCTGGGAG TACGCCTCGC TCTTTGGCTG GAAATTCCAC 
CTGGAGTACC GCAAAACGGA TGCATTCCGT CGCCGCCGCT GGCGCCGCCG CATGGAGCCG 
CTGGAGAAGA CGGGGCCTGC AGCCGTGTTT GCCCTTGAGG GGGCACTGGG TGGCGTGGTA 
GATGACCGGA GTGAAGATTC CATGTCCGTG TCCACCTTGA GCTTTGGCGT GAACAGACCC 
ACGATTTCCT GCATCTTTGA TCATGGGAAC CGCTACCACC TGCGCTGCTA TATGTACCAG 
GCTCGGGACC TGCCCTCGAT GGACAAGGAC TCTTTTTCTG ATCCCTACGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACCGTGGTG GTGAAGAACA CTCTGAACCC CACCTGGGAC 
CAGACGCTCA TCTTCTATGA GATTGAGATC TTTGGAGAGC CAGCCAGCAT CGCCGAGCAG 
CCGCCCAGCA TTGTGGTGGA GCTGTATGAC CACGACACCT ACGGTGCAGA TGAGTTTTTG 
GGACGCTGCA TGTGTCAGCC CAGTCTGGAA CGGATGCCCC GGCTGGCCTG GTTCCCGCTG 
GTTAGGGGCA GCCAGCCGGC AGGCGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG 
AAGCCGGCCA TTCACCATAT TCCTGGTTTT GAGGTGCAGG ATACATCGGG AATCCTGGAC 
GAGTCGGAGG ACACAGACCT GCCCTACCCA CCGCCTCAGA GGGAGGCCAA CATCTACATG 
GTTCCCCAGA ACATCAAGCC GGTTCTCCAG CGCACTGCCA TAGAGATCCT GGCATGGGGC 
CTACGGAACA TGAAGAGTTA CCAGCTGGCC AGTATCTCCT CCCCCAGCCT CGTGGTGGAG 
TGCGGGGGCC AGACGGTGCA GTCCTGTGTC ATCAGGAACC TCCGGAAGAA CCCCAACTTC 
GACATCTGCA CCCTCTTCAT GGAAGTGATG CTGCCCAGAG AGGAGCTCTA CTGTCCCCCC 
ATCGTGATCA AGGTCATTGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT 
ACCATACGCT CCCTGGAGAG CTTCATGTGT GACCTGTACT CCGAGGAGAG TCCGTCCCCA 
CAGGGCGGCC CAGATGATGT GAGTTTACTC AGTCCTGGGG AAGACGTGCT CATTGACATC 
GACGACAAGG AGCCCCTCAT CCCCATACAG CTTACAGACG GTCTGTTGGG CTCTGCCCCC 
ACTAACATGG CATCCTCTCC ATCCAGTCTT CATGAGGAGG AGTTCATCGA CTGGTGGAGC 
AAATTCTTTG CCTCCACAGG GGAGAGGGAG AAGTGCGGCT CGTACTTGGA GAAGGGCTTT 
GACACTCTAA AGGTCTATGA CACACAACTG GAGAACGTGG AGGCCTTTGG GGGCCTGTCT 
GACTTTTGTA ACACCTTCAA GCTCTACCGG GGCAAGACTC AGGAGGAGAC GGAGGACCCA 
TCTGTTATCG GGGAGTTTAA GGGCCTCTTC AAAATCTACC CCCTCCCGGA AGACCCGGCC 
GTCCCCATGC CCCCGAGACA GTTCCAGCAG CTGGCTGCCC AGGGGCCTCA GGACTGCCTG 
GTCCGCATCT ATATCATCCG GGCATTTGGC CTGCAGCCCA AGGACCCCAA TGGGAAGTGT 
GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAATTACATT 
CCCTGTACCC TGGAGCCTGT GTTTGGAAAG ATGTTCGAGC TGACCTGCAC TCTGCCTCTG 
GAGAAGGACC TGAAGATCAC ACTCTACGAC TATGACCTCC TCTCCAAAGA TGAGAAGATC 
GGGGAGACAG TCATCGACCT GGAGAATAGG CTGCTGTCCA AGTTCGGGGC TCGCTGCGGA 
CTCCCACAGA CCTACTGTGT CTCTGGACCC AACCAGTGGA GGGACCAGCT CCGCCCCTCC 
CAGCTTCTCC AGCTCACCTG CCAGCAGCGC AGACTCAAGG CGCCCGTGTA CCTCACAGAC 
CGCGTACTCT TTCAGGATAA AGAATACACC ATTGAGGAAA TAGAGGCTGG CAGGGTCCTG 
AACCCACACC TGGGCCCAGT GGAAGAGCGT CTGGCCTTGC ACGTCCTCCA GCAGCTGGGC 
CTGGTCCCCG AGCATGTGGA GTCCCGGCCT CTTTACAGCC CCCTGCAGCC AGACATTGAG 
CAGGGGAAGC TGCAGATGTG GATCGATCTG TTTCCCAAGG CCCTGGGGCG ACCTGGACCT 
CCCTTCAACA TCACCCCACG GAGAGCAAGA AGGTTTTTCC TGCGTTGTAT TATCTGGAAC 
ACAAAGGACG TGATCCTGGA CGACCTCAGC ATCACGGGGG AGAAGATGAG TGACATTTAC 
GTGAAAGGTT GGATGGTCGG CTTTGAAGAG CACAAGCAGA AGACGGACGT GCACTACCGC 
TCCCTGGGAG GCGAAGGCAA CTTTAACTGG AGGTTCATTT TCCCCTTTGA CTACCTGCCC 
GCTGAGCAAG TCTGCACCGT CTCTAAGAAG GATGCTTTCT GGAGGCTGGA CAAGACTGAG 
AGCAAAATCC CAGCACAAGT GGTATTTCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAC 
GACTTTCTGG GCTCGTTGCA GTTGGATCTC AACCGCATGC CCAAGCCTGC CAAGACAGCT 
GAGAAGTGCT CCTTGGACCA GCTGGACGAC ACTTTCCACC CGGAGTGGTT TGTGTCCCTT 
TTTGAACAGA AAACAGTAAA GGGCTGGTGG CCCTGTGTGG CAGAAGACGG TGAGAAGAAG 
ATCCTGGCGG GCAAGCTGGA AATGACCTTG GAGATCGTAG CAGAGAGTGA GCACGAAGAG 
CGGCCTGCGG GTCAAGGCCG GGATGAACCC AACATGAACC CCAAGCTCGA GGACCCAAGG 
CGCCCCGACA CCTCCTTCCT GTGGTTCACC TCTCCGTACA AGACCATGAA GTTCATCCTG 
TGGCGGCGGT TCCGGTGCGC CATCATCCTC TTCGTCGTTC TCGTCATCCT GCTGCTCTTC 
CTGGGCATCT TCCTCTATTC CTTCCCGAAC TATGCTGCCA TGAAGCTGGT GAAACCCTTC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002914936.1
CDS	150..6515
Translation	MLSCLLVRASNLPSVKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEAQVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPSASLEPSPTLPDMDMVAGGGQSRAETWSLLSDSTVDTRYSGKKWLAPTDTGGEEDTEDQGLTGDEAEPLLDPSGAPGPGAPTTPRKPPSHPPPYHPGSKRKRGAPTPRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVMDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDHLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRLLLSLETKLVEHSDQKVEDLPADDILRVEKYLRRRKYSLFAAFYSASMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADRLEAALEQVHLAVKAQYNAEDVDSLLAQLMDELISDCSQPLGDVHETPSATHLDQYLHRLRSRHLSQIAEAALALKLGHGGLPAALEQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPTHEVLFSRRGASYCGKNCGKLQTIFLKYPMQGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVAGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDTDAGHLSFVAEVFENQTRLPGGHWVYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALRKHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSMSVSTLSFGVNRPTISCIFDHGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFLGRCMCQPSLERMPRLAWFPLVRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTSGILDESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPIVIKVIDNRQFGRRPVVGQCTIRSLESFMCDLYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLTDGLLGSAPTNMASSPSSLHEEEFIDWWSKFFASTGEREKCGSYLEKGFDTLKVYDTQLENVEAFGGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAVPMPPRQFQQLAAQGPQDCLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLQLTCQQRRLKAPVYLTDRVLFQDKEYTIEEIEAGRVLNPHLGPVEERLALHVLQQLGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPAQVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEDGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFVVLVILLLFLGIFLYSFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_002914890.3
Organism	Ailuropoda melanoleuca(giant panda)
Definition	Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002914890.3

ORF Insert Sequence:

ATGCTGAGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGTGAAGAA GGACCGGCGC 
AGTGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCAGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTCCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCGG GAGGTCCTCG CCACCCCCAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCCTC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGTGGCTG GTGGGGGACA GAGCCGGGCT 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACGGGAGAT 
GAGGCAGAGC CATTACTGGA TCCGAGCGGT GCCCCAGGCC CTGGGGCCCC CACCACCCCG 
AGGAAGCCAC CTTCCCATCC GCCCCCCTAC CACCCCGGGA GCAAGAGAAA AAGAGGTGCT 
CCCACGCCCA GAAAGCTGCT GTCGGACAAA CCGCAGGACT TCCAGATCAG GGTGCAAGTG 
ATCGAGGGGC GCCAGCTGCC AGGGGTGAAT ATCAAGCCTG TGGTCAAAGT CACGGCTGCC 
GGGCAGACCA AGAGGACACG GATCCACAAG GGAAACAGCC CACTCTTCAA CGAGACTCTC 
TTCTTCAATG TGTTTGACTC TCCCTCGGAG CTGTTTGACG AGCCCATCTT TATCACGGTG 
GTAGACTCCC GTTCTCTCCG GACAGACGCT CTCATTGGGG AATTCCGGAT GGACGTGGGT 
ACCATCTACA GAGAGCCCCG ACACGCCTAT CTGAGGAAAT GGCTTCTCCT CTCGGACCCT 
GACGATTTCT CTGCTGGGGC CAGAGGTTAC CTGAAAGCAA GCCTCTGTGT GCTGGGGCCC 
GGCGATGAAG CGCCTCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATCGAAAAC 
AATCTGCTCA GGCCAACTGG CGTGGCCCTT CGAGGAGCCC ACTTCTGCCT GAAGGTCTTC 
AGAGCTGAGG ACTTACCACA GATGGATGAT GCCGTGATGG ACAACGTGAA GCAGATCTTC 
GGCTTTGACA GTAACAAGAA GAACTTGGTG GATCCCTTCA TGGAAGTCAG CTTTGCGGGG 
AAAATGCTCT GCAGCAAGAT CCTGGAGAAA ACAGCCAACC CTCAGTGGAA CCAGAGCATC 
ACGCTGCCCG CCATGTTTCC CTCCATGTGT GAAAAAATGA GGATTCGCGT CATGGACTGG 
GACCGCCTCA CCCACAATGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCC 
GCCCCTGGAG GAGAAATAGA AGTGGACGAC CATCTGGGCT TCCTCCCCAC CTTCGGGCCC 
TGCTACGTCA ACCTCTACGG CAGCCCCAGG GAGTTCACCG GCTTCCCGGA CCCCTACGCA 
GAACTCAACA TGGGCAAGGG AGAAGGTGTG GCCTACCGAG GTCGGCTTCT GCTCTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGCGACCAA AAGGTGGAGG ACCTCCCTGC GGATGACATC 
CTTCGGGTGG AGAAGTATCT CCGGAGGCGT AAGTACTCCC TCTTTGCCGC CTTCTACTCG 
GCCTCCATGC TCCAGGATGT GGATGATGCC ATCCAGTTCG AGGTCAGCAT CGGGAACTAT 
GGCAACAAGT TCGACACCAC GTGCCTGCCG CTGGCCTCTA CCACCCAGTA CAGCCGGGCG 
GTCTTTGACG GGTGCCACTA CTACTACTTG CCCTGGGGCA ACGTGAAGCC CGTGGTGGTG 
CTGTCATCCT ACTGGGAGGA CATCAGCCAC AGAGTTGAGG CTCAGAACCA GCTGCTCAGG 
ATTGCGGACC GGCTGGAAGC TGCCCTGGAG CAAGTCCACT TGGCCGTGAA GGCGCAGTAC 
AACGCCGAGG ACGTGGACTC CCTGTTGGCT CAGCTGATGG ATGAGCTCAT CTCAGACTGC 
AGCCAGCCCC TAGGCGATGT CCACGAGACA CCCTCTGCCA CCCACCTGGA CCAGTACCTG 
CACAGGCTGC GTAGCCGTCA CCTAAGCCAG ATTGCAGAGG CGGCGCTGGC CCTGAAGCTC 
GGGCACGGCG GGCTCCCGGC CGCCCTGGAG CAGGCTGAAG ACTGGCTCCT GCGGCTCCGT 
GCCCTGGCCG AGGAGCCTCA GAACAGCCTG CCCGACATCA TCATCTGGAT GCTGCAGGGC 
GACAAGCGAG TGGCATACCA GCGGGTGCCT ACACATGAGG TCCTCTTCTC CCGGCGTGGA 
GCCAGCTACT GCGGCAAGAA CTGTGGGAAG CTGCAGACGA TATTTCTCAA ATATCCCATG 
CAGGGGGTGC CCGGAGCCCG GATGCCCGTG CAGATACGGG TGAAGCTCTG GTTTGGGCTT 
TCTGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGAA AGCTGTCTGT CTTTGCTGAA 
ACCTATGAGA ACCAGACCAA ACTGGCCCTC GTTGGGAACT GGGGCACAAC AGGCCTTACC 
TACCCCAAGT TTTCTGATGT CGCGGGCAAG ATCAAGCTCC CCAAGGATAG CTTCCGCCCC 
TCAGCCGGCT GGACCTGGGC TGGAGATTGG TTTGTGTGTC CAGAGAAGAC GCTGCTCCAT 
GACACCGACG CGGGCCACCT GAGTTTCGTG GCGGAGGTGT TTGAGAACCA GACCCGGCTC 
CCTGGAGGCC ACTGGGTCTA CATGAGTGAC AACTACACGG ATGTGAATGG GGAGAAGGTG 
CTTCCCAAGG ATGACATTGA GTGCCCGCTG GGCTGGAAGT GGGAAGATGA AGAGTGGTCC 
ACGGACCTCA ATCGGGCCGT TGATGAGCAA GGCTGGGAGT ACAGTATCAC CATCCCCCCG 
GACCGGAAGC CGAAGCACTG GGTCCCCGCT GAGAAGATGT ACTACACGCA CCGTCGGCGG 
CGCTGGGTCC GCCTGCGCAG GAGGGACCTC AGCCAGATGG AGGCCCTGAG GAAGCACAGG 
CAGGCGGAGG CGGAGGGCGA GGGCTGGGAG TACGCCTCGC TCTTTGGCTG GAAATTCCAC 
CTGGAGTACC GCAAAACGGA TGCATTCCGT CGCCGCCGCT GGCGCCGCCG CATGGAGCCG 
CTGGAGAAGA CGGGGCCTGC AGCCGTGTTT GCCCTTGAGG GGGCACTGGG TGGCGTGGTA 
GATGACCGGA GTGAAGATTC CATGTCCGTG TCCACCTTGA GCTTTGGCGT GAACAGACCC 
ACGATTTCCT GCATCTTTGA TCATGGGAAC CGCTACCACC TGCGCTGCTA TATGTACCAG 
GCTCGGGACC TGCCCTCGAT GGACAAGGAC TCTTTTTCTG ATCCCTACGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACCGTGGTG GTGAAGAACA CTCTGAACCC CACCTGGGAC 
CAGACGCTCA TCTTCTATGA GATTGAGATC TTTGGAGAGC CAGCCAGCAT CGCCGAGCAG 
CCGCCCAGCA TTGTGGTGGA GCTGTATGAC CACGACACCT ACGGTGCAGA TGAGTTTTTG 
GGACGCTGCA TGTGTCAGCC CAGTCTGGAA CGGATGCCCC GGCTGGCCTG GTTCCCGCTG 
GTTAGGGGCA GCCAGCCGGC AGGCGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG 
AAGCCGGCCA TTCACCATAT TCCTGGTTTT GAGGTGCAGG ATACATCGGG AATCCTGGAC 
GAGTCGGAGG ACACAGACCT GCCCTACCCA CCGCCTCAGA GGGAGGCCAA CATCTACATG 
GTTCCCCAGA ACATCAAGCC GGTTCTCCAG CGCACTGCCA TAGAGATCCT GGCATGGGGC 
CTACGGAACA TGAAGAGTTA CCAGCTGGCC AGTATCTCCT CCCCCAGCCT CGTGGTGGAG 
TGCGGGGGCC AGACGGTGCA GTCCTGTGTC ATCAGGAACC TCCGGAAGAA CCCCAACTTC 
GACATCTGCA CCCTCTTCAT GGAAGTGATG CTGCCCAGAG AGGAGCTCTA CTGTCCCCCC 
ATCGTGATCA AGGTCATTGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT 
ACCATACGCT CCCTGGAGAG CTTCATGTGT GACCTGTACT CCGAGGAGAG TCCGTCCCCA 
CAGGGCGGCC CAGATGATGT GAGTTTACTC AGTCCTGGGG AAGACGTGCT CATTGACATC 
GACGACAAGG AGCCCCTCAT CCCCATACAG CTTACAGACG GTCTGTTGGG CTCTGCCCCC 
ACTAACATGG CATCCTCTCC ATCCAGTCTT CATGAGGAGG AGTTCATCGA CTGGTGGAGC 
AAATTCTTTG CCTCCACAGG GGAGAGGGAG AAGTGCGGCT CGTACTTGGA GAAGGGCTTT 
GACACTCTAA AGGTCTATGA CACACAACTG GAGAACGTGG AGGCCTTTGG GGGCCTGTCT 
GACTTTTGTA ACACCTTCAA GCTCTACCGG GGCAAGACTC AGGAGGAGAC GGAGGACCCA 
TCTGTTATCG GGGAGTTTAA GGGCCTCTTC AAAATCTACC CCCTCCCGGA AGACCCGGCC 
GTCCCCATGC CCCCGAGACA GTTCCAGCAG CTGGCTGCCC AGGGGCCTCA GGACTGCCTG 
GTCCGCATCT ATATCATCCG GGCATTTGGC CTGCAGCCCA AGGACCCCAA TGGGAAGTGT 
GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAATTACATT 
CCCTGTACCC TGGAGCCTGT GTTTGGAAAG ATGTTCGAGC TGACCTGCAC TCTGCCTCTG 
GAGAAGGACC TGAAGATCAC ACTCTACGAC TATGACCTCC TCTCCAAAGA TGAGAAGATC 
GGGGAGACAG TCATCGACCT GGAGAATAGG CTGCTGTCCA AGTTCGGGGC TCGCTGCGGA 
CTCCCACAGA CCTACTGTGT CTCTGGACCC AACCAGTGGA GGGACCAGCT CCGCCCCTCC 
CAGCTTCTCC AGCTCACCTG CCAGCAGCGC AGACTCAAGG CGCCCGTGTA CCTCACAGAC 
CGCGTACTCT TTCAGGATAA AGAATACACC ATTGAGGAAA TAGAGGCTGG CAGGGTCCTG 
AACCCACACC TGGGCCCAGT GGAAGAGCGT CTGGCCTTGC ACGTCCTCCA GCAGCTGGGC 
CTGGTCCCCG AGCATGTGGA GTCCCGGCCT CTTTACAGCC CCCTGCAGCC AGACATTGAG 
CAGGGGAAGC TGCAGATGTG GATCGATCTG TTTCCCAAGG CCCTGGGGCG ACCTGGACCT 
CCCTTCAACA TCACCCCACG GAGAGCAAGA AGGTTTTTCC TGCGTTGTAT TATCTGGAAC 
ACAAAGGACG TGATCCTGGA CGACCTCAGC ATCACGGGGG AGAAGATGAG TGACATTTAC 
GTGAAAGGTT GGATGGTCGG CTTTGAAGAG CACAAGCAGA AGACGGACGT GCACTACCGC 
TCCCTGGGAG GCGAAGGCAA CTTTAACTGG AGGTTCATTT TCCCCTTTGA CTACCTGCCC 
GCTGAGCAAG TCTGCACCGT CTCTAAGAAG GATGCTTTCT GGAGGCTGGA CAAGACTGAG 
AGCAAAATCC CAGCACAAGT GGTATTTCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAC 
GACTTTCTGG GCTCGTTGCA GTTGGATCTC AACCGCATGC CCAAGCCTGC CAAGACAGCT 
GAGAAGTGCT CCTTGGACCA GCTGGACGAC ACTTTCCACC CGGAGTGGTT TGTGTCCCTT 
TTTGAACAGA AAACAGTAAA GGGCTGGTGG CCCTGTGTGG CAGAAGACGG TGAGAAGAAG 
ATCCTGGCGG GCAAGCTGGA AATGACCTTG GAGATCGTAG CAGAGAGTGA GCACGAAGAG 
CGGCCTGCGG GTCAAGGCCG GGATGAACCC AACATGAACC CCAAGCTCGA GGACCCAAGG 
CGCCCCGACA CCTCCTTCCT GTGGTTCACC TCTCCGTACA AGACCATGAA GTTCATCCTG 
TGGCGGCGGT TCCGGTGCGC CATCATCCTC TTCGTCGTTC TCGTCATCCT GCTGCTCTTC 
CTGGGCATCT TCCTCTATTC CTTCCCGAAC TATGCTGCCA TGAAGCTGGT GAAACCCTTC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_002914892.3 ) cDNA ORF clone, Ailuropoda melanoleuca(giant panda) -> XP_002914938.1 Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X14, mRNA.

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CloneID	OAc02559
Clone ID Related Accession (Same CDS sequence)	XM_002914892.3
Accession Version	XM_002914892.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-27
Organism	Ailuropoda melanoleuca(giant panda)
Product	dysferlin isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003217356.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 28, 2016 this sequence version replaced XM_002914892.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ailuropoda melanoleuca Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGTGAAGAA GGACCGGCGC 
AGTGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCAGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTCCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCGG GAGGTCCTCG CCACCCCCAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCCTC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGTGGCTG GTGGGGGACA GAGCCGGGCT 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACGGGAGAT 
GAGGCAGAGC CATTACTGGA TCCGAGCGGT GCCCCAGGCC CTGGGGCCCC CACCACCCCG 
AGGAAGCCAC CTTCCCATCC GCCCCCCTAC CACCCCGGGA GCAAGAGAAA AAGAGGTGCT 
CCCACGCCCA GAAAGCTGCT GTCGGACAAA CCGCAGGACT TCCAGATCAG GGTGCAAGTG 
ATCGAGGGGC GCCAGCTGCC AGGGGTGAAT ATCAAGCCTG TGGTCAAAGT CACGGCTGCC 
GGGCAGACCA AGAGGACACG GATCCACAAG GGAAACAGCC CACTCTTCAA CGAGACTCTC 
TTCTTCAATG TGTTTGACTC TCCCTCGGAG CTGTTTGACG AGCCCATCTT TATCACGGTG 
GTAGACTCCC GTTCTCTCCG GACAGACGCT CTCATTGGGG AATTCCGGAT GGACGTGGGT 
ACCATCTACA GAGAGCCCCG ACACGCCTAT CTGAGGAAAT GGCTTCTCCT CTCGGACCCT 
GACGATTTCT CTGCTGGGGC CAGAGGTTAC CTGAAAGCAA GCCTCTGTGT GCTGGGGCCC 
GGCGATGAAG CGCCTCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATCGAAAAC 
AATCTGCTCA GGCCAACTGG CGTGGCCCTT CGAGGAGCCC ACTTCTGCCT GAAGGTCTTC 
AGAGCTGAGG ACTTACCACA GATGGATGAT GCCGTGATGG ACAACGTGAA GCAGATCTTC 
GGCTTTGACA GTAACAAGAA GAACTTGGTG GATCCCTTCA TGGAAGTCAG CTTTGCGGGG 
AAAATGCTCT GCAGCAAGAT CCTGGAGAAA ACAGCCAACC CTCAGTGGAA CCAGAGCATC 
ACGCTGCCCG CCATGTTTCC CTCCATGTGT GAAAAAATGA GGATTCGCGT CATGGACTGG 
GACCGCCTCA CCCACAATGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCC 
GCCCCTGGAG GAGAAATAGA AGTGGACGAC CATCTGGGCT TCCTCCCCAC CTTCGGGCCC 
TGCTACGTCA ACCTCTACGG CAGCCCCAGG GAGTTCACCG GCTTCCCGGA CCCCTACGCA 
GAACTCAACA TGGGCAAGGG AGAAGGTGTG GCCTACCGAG GTCGGCTTCT GCTCTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGCGACCAA AAGGTGGAGG ACCTCCCTGC GGATGACATC 
CTTCGGGTGG AGAAGTATCT CCGGAGGCGT AAGTACTCCC TCTTTGCCGC CTTCTACTCG 
GCCTCCATGC TCCAGGATGT GGATGATGCC ATCCAGTTCG AGGTCAGCAT CGGGAACTAT 
GGCAACAAGT TCGACACCAC GTGCCTGCCG CTGGCCTCTA CCACCCAGTA CAGCCGGGCG 
GTCTTTGACG GGTGCCACTA CTACTACTTG CCCTGGGGCA ACGTGAAGCC CGTGGTGGTG 
CTGTCATCCT ACTGGGAGGA CATCAGCCAC AGAGTTGAGG CTCAGAACCA GCTGCTCAGG 
ATTGCGGACC GGCTGGAAGC TGCCCTGGAG CAAGTCCACT TGGCCGTGAA GGCGCAGTAC 
AACGCCGAGG ACGTGGACTC CCTGTTGGCT CAGCTGATGG ATGAGCTCAT CTCAGACTGC 
AGCCAGCCCC TAGGCGATGT CCACGAGACA CCCTCTGCCA CCCACCTGGA CCAGTACCTG 
CACAGGCTGC GTAGCCGTCA CCTAAGCCAG ATTGCAGAGG CGGCGCTGGC CCTGAAGCTC 
GGGCACGGCG GGCTCCCGGC CGCCCTGGAG CAGGCTGAAG ACTGGCTCCT GCGGCTCCGT 
GCCCTGGCCG AGGAGCCTCA GAACAGCCTG CCCGACATCA TCATCTGGAT GCTGCAGGGC 
GACAAGCGAG TGGCATACCA GCGGGTGCCT ACACATGAGG TCCTCTTCTC CCGGCGTGGA 
GCCAGCTACT GCGGCAAGAA CTGTGGGAAG CTGCAGACGA TATTTCTCAA ATATCCCATG 
CAGGGGGTGC CCGGAGCCCG GATGCCCGTG CAGATACGGG TGAAGCTCTG GTTTGGGCTT 
TCTGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGAA AGCTGTCTGT CTTTGCTGAA 
ACCTATGAGA ACCAGACCAA ACTGGCCCTC GTTGGGAACT GGGGCACAAC AGGCCTTACC 
TACCCCAAGT TTTCTGATGT CGCGGGCAAG ATCAAGCTCC CCAAGGATAG CTTCCGCCCC 
TCAGCCGGCT GGACCTGGGC TGGAGATTGG TTTGTGTGTC CAGAGAAGAC GCTGCTCCAT 
GACACCGACG CGGGCCACCT GAGTTTCGTG GCGGAGGTGT TTGAGAACCA GACCCGGCTC 
CCTGGAGGCC ACTGGGTCTA CATGAGTGAC AACTACACGG ATGTGAATGG GGAGAAGGTG 
CTTCCCAAGG ATGACATTGA GTGCCCGCTG GGCTGGAAGT GGGAAGATGA AGAGTGGTCC 
ACGGACCTCA ATCGGGCCGT TGATGAGCAA GGCTGGGAGT ACAGTATCAC CATCCCCCCG 
GACCGGAAGC CGAAGCACTG GGTCCCCGCT GAGAAGATGT ACTACACGCA CCGTCGGCGG 
CGCTGGGTCC GCCTGCGCAG GAGGGACCTC AGCCAGATGG AGGCCCTGAG GAAGCACAGG 
CAGGCGGAGG CGGAGGGCGA GGGCTGGGAG TACGCCTCGC TCTTTGGCTG GAAATTCCAC 
CTGGAGTACC GCAAAACGGA TGCATTCCGT CGCCGCCGCT GGCGCCGCCG CATGGAGCCG 
CTGGAGAAGA CGGGGCCTGC AGCCGTGTTT GCCCTTGAGG GGGCACTGGG TGGCGTGGTA 
GATGACCGGA GTGAAGATTC CATGTCCGTG TCCACCTTGA GCTTTGGCGT GAACAGACCC 
ACGATTTCCT GCATCTTTGA TCATGGGAAC CGCTACCACC TGCGCTGCTA TATGTACCAG 
GCTCGGGACC TGCCCTCGAT GGACAAGGAC TCTTTTTCTG ATCCCTACGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACCGTGGTG GTGAAGAACA CTCTGAACCC CACCTGGGAC 
CAGACGCTCA TCTTCTATGA GATTGAGATC TTTGGAGAGC CAGCCAGCAT CGCCGAGCAG 
CCGCCCAGCA TTGTGGTGGA GCTGTATGAC CACGACACCT ACGGTGCAGA TGAGTTTTTG 
GGACGCTGCA TGTGTCAGCC CAGTCTGGAA CGGATGCCCC GGCTGGCCTG GTTCCCGCTG 
GTTAGGGGCA GCCAGCCGGC AGGCGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG 
AAGCCGGCCA TTCACCATAT TCCTGGTTTT GAGGTGCAGG ATACATCGGG AATCCTGGAC 
GAGTCGGAGG ACACAGACCT GCCCTACCCA CCGCCTCAGA GGGAGGCCAA CATCTACATG 
GTTCCCCAGA ACATCAAGCC GGTTCTCCAG CGCACTGCCA TAGAGATCCT GGCATGGGGC 
CTACGGAACA TGAAGAGTTA CCAGCTGGCC AGTATCTCCT CCCCCAGCCT CGTGGTGGAG 
TGCGGGGGCC AGACGGTGCA GTCCTGTGTC ATCAGGAACC TCCGGAAGAA CCCCAACTTC 
GACATCTGCA CCCTCTTCAT GGAAGTGATG CTGCCCAGAG AGGAGCTCTA CTGTCCCCCC 
ATCGTGATCA AGGTCATTGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT 
ACCATACGCT CCCTGGAGAG CTTCATGTGT GACCTGTACT CCGAGGAGAG TCCGTCCCCA 
CAGGGCGGCC CAGATGATGT GAGTTTACTC AGTCCTGGGG AAGACGTGCT CATTGACATC 
GACGACAAGG AGCCCCTCAT CCCCATACAG GAGGAGGAGT TCATCGACTG GTGGAGCAAA 
TTCTTTGCCT CCACAGGGGA GAGGGAGAAG TGCGGCTCGT ACTTGGAGAA GGGCTTTGAC 
ACTCTAAAGG TCTATGACAC ACAACTGGAG AACGTGGAGG CCTTTGGGGG CCTGTCTGAC 
TTTTGTAACA CCTTCAAGCT CTACCGGGGC AAGACTCAGG AGGAGACGGA GGACCCATCT 
GTTATCGGGG AGTTTAAGGG CCTCTTCAAA ATCTACCCCC TCCCGGAAGA CCCGGCCGTC 
CCCATGCCCC CGAGACAGTT CCAGCAGCTG GCTGCCCAGG GGCCTCAGGA CTGCCTGGTC 
CGCATCTATA TCATCCGGGC ATTTGGCCTG CAGCCCAAGG ACCCCAATGG GAAGTGTGAT 
CCTTACATCA AGATCTCCAT AGGGAAGAAA TCAGTGAGTG ACCAGGATAA TTACATTCCC 
TGTACCCTGG AGCCTGTGTT TGGAAAGATG TTCGAGCTGA CCTGCACTCT GCCTCTGGAG 
AAGGACCTGA AGATCACACT CTACGACTAT GACCTCCTCT CCAAAGATGA GAAGATCGGG 
GAGACAGTCA TCGACCTGGA GAATAGGCTG CTGTCCAAGT TCGGGGCTCG CTGCGGACTC 
CCACAGACCT ACTGTGTCTC TGGACCCAAC CAGTGGAGGG ACCAGCTCCG CCCCTCCCAG 
CTTCTCCAGC TCACCTGCCA GCAGCGCAGA CTCAAGGCGC CCGTGTACCT CACAGACCGC 
GTACTCTTTC AGGATAAAGA ATACACCATT GAGGAAATAG AGGCTGGCAG GGTCCTGAAC 
CCACACCTGG GCCCAGTGGA AGAGCGTCTG GCCTTGCACG TCCTCCAGCA GCTGGGCCTG 
GTCCCCGAGC ATGTGGAGTC CCGGCCTCTT TACAGCCCCC TGCAGCCAGA CATTGAGCAG 
GGGAAGCTGC AGATGTGGAT CGATCTGTTT CCCAAGGCCC TGGGGCGACC TGGACCTCCC 
TTCAACATCA CCCCACGGAG AGCAAGAAGG TTTTTCCTGC GTTGTATTAT CTGGAACACA 
AAGGACGTGA TCCTGGACGA CCTCAGCATC ACGGGGGAGA AGATGAGTGA CATTTACGTG 
AAAGGTTGGA TGGTCGGCTT TGAAGAGCAC AAGCAGAAGA CGGACGTGCA CTACCGCTCC 
CTGGGAGGCG AAGGCAACTT TAACTGGAGG TTCATTTTCC CCTTTGACTA CCTGCCCGCT 
GAGCAAGTCT GCACCGTCTC TAAGAAGGAT GCTTTCTGGA GGCTGGACAA GACTGAGAGC 
AAAATCCCAG CACAAGTGGT ATTTCAGATC TGGGACAATG ACAAGTTCTC CTTTGACGAC 
TTTCTGGGCT CGTTGCAGTT GGATCTCAAC CGCATGCCCA AGCCTGCCAA GACAGCTGAG 
AAGTGCTCCT TGGACCAGCT GGACGACACT TTCCACCCGG AGTGGTTTGT GTCCCTTTTT 
GAACAGAAAA CAGTAAAGGG CTGGTGGCCC TGTGTGGCAG AAGACGGTGA GAAGAAGATC 
CTGGCGGGCA AGCTGGAAAT GACCTTGGAG ATCGTAGCAG AGAGTGAGCA CGAAGAGCGG 
CCTGCGGGTC AAGGCCGGGA TGAACCCAAC ATGAACCCCA AGCTCGAGGA CCCAAGGCGC 
CCCGACACCT CCTTCCTGTG GTTCACCTCT CCGTACAAGA CCATGAAGTT CATCCTGTGG 
CGGCGGTTCC GGTGCGCCAT CATCCTCTTC GTCGTTCTCG TCATCCTGCT GCTCTTCCTG 
GGCATCTTCC TCTATTCCTT CCCGAACTAT GCTGCCATGA AGCTGGTGAA ACCCTTCAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002914938.1
CDS	150..6452
Translation	MLSCLLVRASNLPSVKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEAQVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPSASLEPSPTLPDMDMVAGGGQSRAETWSLLSDSTVDTRYSGKKWLAPTDTGGEEDTEDQGLTGDEAEPLLDPSGAPGPGAPTTPRKPPSHPPPYHPGSKRKRGAPTPRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVMDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDHLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRLLLSLETKLVEHSDQKVEDLPADDILRVEKYLRRRKYSLFAAFYSASMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADRLEAALEQVHLAVKAQYNAEDVDSLLAQLMDELISDCSQPLGDVHETPSATHLDQYLHRLRSRHLSQIAEAALALKLGHGGLPAALEQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPTHEVLFSRRGASYCGKNCGKLQTIFLKYPMQGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVAGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDTDAGHLSFVAEVFENQTRLPGGHWVYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALRKHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSMSVSTLSFGVNRPTISCIFDHGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFLGRCMCQPSLERMPRLAWFPLVRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTSGILDESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPIVIKVIDNRQFGRRPVVGQCTIRSLESFMCDLYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASTGEREKCGSYLEKGFDTLKVYDTQLENVEAFGGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAVPMPPRQFQQLAAQGPQDCLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLQLTCQQRRLKAPVYLTDRVLFQDKEYTIEEIEAGRVLNPHLGPVEERLALHVLQQLGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPAQVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEDGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFVVLVILLLFLGIFLYSFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_002914892.3
Organism	Ailuropoda melanoleuca(giant panda)
Definition	Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002914892.3

ORF Insert Sequence:

ATGCTGAGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGTGAAGAA GGACCGGCGC 
AGTGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCAGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTCCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCGG GAGGTCCTCG CCACCCCCAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCCTC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGTGGCTG GTGGGGGACA GAGCCGGGCT 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACGGGAGAT 
GAGGCAGAGC CATTACTGGA TCCGAGCGGT GCCCCAGGCC CTGGGGCCCC CACCACCCCG 
AGGAAGCCAC CTTCCCATCC GCCCCCCTAC CACCCCGGGA GCAAGAGAAA AAGAGGTGCT 
CCCACGCCCA GAAAGCTGCT GTCGGACAAA CCGCAGGACT TCCAGATCAG GGTGCAAGTG 
ATCGAGGGGC GCCAGCTGCC AGGGGTGAAT ATCAAGCCTG TGGTCAAAGT CACGGCTGCC 
GGGCAGACCA AGAGGACACG GATCCACAAG GGAAACAGCC CACTCTTCAA CGAGACTCTC 
TTCTTCAATG TGTTTGACTC TCCCTCGGAG CTGTTTGACG AGCCCATCTT TATCACGGTG 
GTAGACTCCC GTTCTCTCCG GACAGACGCT CTCATTGGGG AATTCCGGAT GGACGTGGGT 
ACCATCTACA GAGAGCCCCG ACACGCCTAT CTGAGGAAAT GGCTTCTCCT CTCGGACCCT 
GACGATTTCT CTGCTGGGGC CAGAGGTTAC CTGAAAGCAA GCCTCTGTGT GCTGGGGCCC 
GGCGATGAAG CGCCTCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATCGAAAAC 
AATCTGCTCA GGCCAACTGG CGTGGCCCTT CGAGGAGCCC ACTTCTGCCT GAAGGTCTTC 
AGAGCTGAGG ACTTACCACA GATGGATGAT GCCGTGATGG ACAACGTGAA GCAGATCTTC 
GGCTTTGACA GTAACAAGAA GAACTTGGTG GATCCCTTCA TGGAAGTCAG CTTTGCGGGG 
AAAATGCTCT GCAGCAAGAT CCTGGAGAAA ACAGCCAACC CTCAGTGGAA CCAGAGCATC 
ACGCTGCCCG CCATGTTTCC CTCCATGTGT GAAAAAATGA GGATTCGCGT CATGGACTGG 
GACCGCCTCA CCCACAATGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCC 
GCCCCTGGAG GAGAAATAGA AGTGGACGAC CATCTGGGCT TCCTCCCCAC CTTCGGGCCC 
TGCTACGTCA ACCTCTACGG CAGCCCCAGG GAGTTCACCG GCTTCCCGGA CCCCTACGCA 
GAACTCAACA TGGGCAAGGG AGAAGGTGTG GCCTACCGAG GTCGGCTTCT GCTCTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGCGACCAA AAGGTGGAGG ACCTCCCTGC GGATGACATC 
CTTCGGGTGG AGAAGTATCT CCGGAGGCGT AAGTACTCCC TCTTTGCCGC CTTCTACTCG 
GCCTCCATGC TCCAGGATGT GGATGATGCC ATCCAGTTCG AGGTCAGCAT CGGGAACTAT 
GGCAACAAGT TCGACACCAC GTGCCTGCCG CTGGCCTCTA CCACCCAGTA CAGCCGGGCG 
GTCTTTGACG GGTGCCACTA CTACTACTTG CCCTGGGGCA ACGTGAAGCC CGTGGTGGTG 
CTGTCATCCT ACTGGGAGGA CATCAGCCAC AGAGTTGAGG CTCAGAACCA GCTGCTCAGG 
ATTGCGGACC GGCTGGAAGC TGCCCTGGAG CAAGTCCACT TGGCCGTGAA GGCGCAGTAC 
AACGCCGAGG ACGTGGACTC CCTGTTGGCT CAGCTGATGG ATGAGCTCAT CTCAGACTGC 
AGCCAGCCCC TAGGCGATGT CCACGAGACA CCCTCTGCCA CCCACCTGGA CCAGTACCTG 
CACAGGCTGC GTAGCCGTCA CCTAAGCCAG ATTGCAGAGG CGGCGCTGGC CCTGAAGCTC 
GGGCACGGCG GGCTCCCGGC CGCCCTGGAG CAGGCTGAAG ACTGGCTCCT GCGGCTCCGT 
GCCCTGGCCG AGGAGCCTCA GAACAGCCTG CCCGACATCA TCATCTGGAT GCTGCAGGGC 
GACAAGCGAG TGGCATACCA GCGGGTGCCT ACACATGAGG TCCTCTTCTC CCGGCGTGGA 
GCCAGCTACT GCGGCAAGAA CTGTGGGAAG CTGCAGACGA TATTTCTCAA ATATCCCATG 
CAGGGGGTGC CCGGAGCCCG GATGCCCGTG CAGATACGGG TGAAGCTCTG GTTTGGGCTT 
TCTGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGAA AGCTGTCTGT CTTTGCTGAA 
ACCTATGAGA ACCAGACCAA ACTGGCCCTC GTTGGGAACT GGGGCACAAC AGGCCTTACC 
TACCCCAAGT TTTCTGATGT CGCGGGCAAG ATCAAGCTCC CCAAGGATAG CTTCCGCCCC 
TCAGCCGGCT GGACCTGGGC TGGAGATTGG TTTGTGTGTC CAGAGAAGAC GCTGCTCCAT 
GACACCGACG CGGGCCACCT GAGTTTCGTG GCGGAGGTGT TTGAGAACCA GACCCGGCTC 
CCTGGAGGCC ACTGGGTCTA CATGAGTGAC AACTACACGG ATGTGAATGG GGAGAAGGTG 
CTTCCCAAGG ATGACATTGA GTGCCCGCTG GGCTGGAAGT GGGAAGATGA AGAGTGGTCC 
ACGGACCTCA ATCGGGCCGT TGATGAGCAA GGCTGGGAGT ACAGTATCAC CATCCCCCCG 
GACCGGAAGC CGAAGCACTG GGTCCCCGCT GAGAAGATGT ACTACACGCA CCGTCGGCGG 
CGCTGGGTCC GCCTGCGCAG GAGGGACCTC AGCCAGATGG AGGCCCTGAG GAAGCACAGG 
CAGGCGGAGG CGGAGGGCGA GGGCTGGGAG TACGCCTCGC TCTTTGGCTG GAAATTCCAC 
CTGGAGTACC GCAAAACGGA TGCATTCCGT CGCCGCCGCT GGCGCCGCCG CATGGAGCCG 
CTGGAGAAGA CGGGGCCTGC AGCCGTGTTT GCCCTTGAGG GGGCACTGGG TGGCGTGGTA 
GATGACCGGA GTGAAGATTC CATGTCCGTG TCCACCTTGA GCTTTGGCGT GAACAGACCC 
ACGATTTCCT GCATCTTTGA TCATGGGAAC CGCTACCACC TGCGCTGCTA TATGTACCAG 
GCTCGGGACC TGCCCTCGAT GGACAAGGAC TCTTTTTCTG ATCCCTACGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACCGTGGTG GTGAAGAACA CTCTGAACCC CACCTGGGAC 
CAGACGCTCA TCTTCTATGA GATTGAGATC TTTGGAGAGC CAGCCAGCAT CGCCGAGCAG 
CCGCCCAGCA TTGTGGTGGA GCTGTATGAC CACGACACCT ACGGTGCAGA TGAGTTTTTG 
GGACGCTGCA TGTGTCAGCC CAGTCTGGAA CGGATGCCCC GGCTGGCCTG GTTCCCGCTG 
GTTAGGGGCA GCCAGCCGGC AGGCGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG 
AAGCCGGCCA TTCACCATAT TCCTGGTTTT GAGGTGCAGG ATACATCGGG AATCCTGGAC 
GAGTCGGAGG ACACAGACCT GCCCTACCCA CCGCCTCAGA GGGAGGCCAA CATCTACATG 
GTTCCCCAGA ACATCAAGCC GGTTCTCCAG CGCACTGCCA TAGAGATCCT GGCATGGGGC 
CTACGGAACA TGAAGAGTTA CCAGCTGGCC AGTATCTCCT CCCCCAGCCT CGTGGTGGAG 
TGCGGGGGCC AGACGGTGCA GTCCTGTGTC ATCAGGAACC TCCGGAAGAA CCCCAACTTC 
GACATCTGCA CCCTCTTCAT GGAAGTGATG CTGCCCAGAG AGGAGCTCTA CTGTCCCCCC 
ATCGTGATCA AGGTCATTGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT 
ACCATACGCT CCCTGGAGAG CTTCATGTGT GACCTGTACT CCGAGGAGAG TCCGTCCCCA 
CAGGGCGGCC CAGATGATGT GAGTTTACTC AGTCCTGGGG AAGACGTGCT CATTGACATC 
GACGACAAGG AGCCCCTCAT CCCCATACAG GAGGAGGAGT TCATCGACTG GTGGAGCAAA 
TTCTTTGCCT CCACAGGGGA GAGGGAGAAG TGCGGCTCGT ACTTGGAGAA GGGCTTTGAC 
ACTCTAAAGG TCTATGACAC ACAACTGGAG AACGTGGAGG CCTTTGGGGG CCTGTCTGAC 
TTTTGTAACA CCTTCAAGCT CTACCGGGGC AAGACTCAGG AGGAGACGGA GGACCCATCT 
GTTATCGGGG AGTTTAAGGG CCTCTTCAAA ATCTACCCCC TCCCGGAAGA CCCGGCCGTC 
CCCATGCCCC CGAGACAGTT CCAGCAGCTG GCTGCCCAGG GGCCTCAGGA CTGCCTGGTC 
CGCATCTATA TCATCCGGGC ATTTGGCCTG CAGCCCAAGG ACCCCAATGG GAAGTGTGAT 
CCTTACATCA AGATCTCCAT AGGGAAGAAA TCAGTGAGTG ACCAGGATAA TTACATTCCC 
TGTACCCTGG AGCCTGTGTT TGGAAAGATG TTCGAGCTGA CCTGCACTCT GCCTCTGGAG 
AAGGACCTGA AGATCACACT CTACGACTAT GACCTCCTCT CCAAAGATGA GAAGATCGGG 
GAGACAGTCA TCGACCTGGA GAATAGGCTG CTGTCCAAGT TCGGGGCTCG CTGCGGACTC 
CCACAGACCT ACTGTGTCTC TGGACCCAAC CAGTGGAGGG ACCAGCTCCG CCCCTCCCAG 
CTTCTCCAGC TCACCTGCCA GCAGCGCAGA CTCAAGGCGC CCGTGTACCT CACAGACCGC 
GTACTCTTTC AGGATAAAGA ATACACCATT GAGGAAATAG AGGCTGGCAG GGTCCTGAAC 
CCACACCTGG GCCCAGTGGA AGAGCGTCTG GCCTTGCACG TCCTCCAGCA GCTGGGCCTG 
GTCCCCGAGC ATGTGGAGTC CCGGCCTCTT TACAGCCCCC TGCAGCCAGA CATTGAGCAG 
GGGAAGCTGC AGATGTGGAT CGATCTGTTT CCCAAGGCCC TGGGGCGACC TGGACCTCCC 
TTCAACATCA CCCCACGGAG AGCAAGAAGG TTTTTCCTGC GTTGTATTAT CTGGAACACA 
AAGGACGTGA TCCTGGACGA CCTCAGCATC ACGGGGGAGA AGATGAGTGA CATTTACGTG 
AAAGGTTGGA TGGTCGGCTT TGAAGAGCAC AAGCAGAAGA CGGACGTGCA CTACCGCTCC 
CTGGGAGGCG AAGGCAACTT TAACTGGAGG TTCATTTTCC CCTTTGACTA CCTGCCCGCT 
GAGCAAGTCT GCACCGTCTC TAAGAAGGAT GCTTTCTGGA GGCTGGACAA GACTGAGAGC 
AAAATCCCAG CACAAGTGGT ATTTCAGATC TGGGACAATG ACAAGTTCTC CTTTGACGAC 
TTTCTGGGCT CGTTGCAGTT GGATCTCAAC CGCATGCCCA AGCCTGCCAA GACAGCTGAG 
AAGTGCTCCT TGGACCAGCT GGACGACACT TTCCACCCGG AGTGGTTTGT GTCCCTTTTT 
GAACAGAAAA CAGTAAAGGG CTGGTGGCCC TGTGTGGCAG AAGACGGTGA GAAGAAGATC 
CTGGCGGGCA AGCTGGAAAT GACCTTGGAG ATCGTAGCAG AGAGTGAGCA CGAAGAGCGG 
CCTGCGGGTC AAGGCCGGGA TGAACCCAAC ATGAACCCCA AGCTCGAGGA CCCAAGGCGC 
CCCGACACCT CCTTCCTGTG GTTCACCTCT CCGTACAAGA CCATGAAGTT CATCCTGTGG 
CGGCGGTTCC GGTGCGCCAT CATCCTCTTC GTCGTTCTCG TCATCCTGCT GCTCTTCCTG 
GGCATCTTCC TCTATTCCTT CCCGAACTAT GCTGCCATGA AGCTGGTGAA ACCCTTCAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_002914893.3 ) cDNA ORF clone, Ailuropoda melanoleuca(giant panda) -> XP_002914939.1 Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X16, mRNA.

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CloneID	OAc02560
Clone ID Related Accession (Same CDS sequence)	XM_002914893.3
Accession Version	XM_002914893.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-27
Organism	Ailuropoda melanoleuca(giant panda)
Product	dysferlin isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003217356.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 28, 2016 this sequence version replaced XM_002914893.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ailuropoda melanoleuca Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGTGAAGAA GGACCGGCGC 
AGTGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCAGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTCCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCGG GAGGTCCTCG CCACCCCCAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCCTC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGTGGCTG ACACAGGAGG AGAGGAGGAC 
ACTGAGGACC AGGGGCTCAC GGGAGATGAG GCAGAGCCAT TACTGGATCC GAGCGGTGCC 
CCAGGCCCTG GGGCCCCCAC CACCCCGAGG AAGCCACCTT CCCATCCGCC CCCCTACCAC 
CCCGGGAGCA AGAGAAAAAG AGGTGCTCCC ACGCCCAGAA AGCTGCTGTC GGACAAACCG 
CAGGACTTCC AGATCAGGGT GCAAGTGATC GAGGGGCGCC AGCTGCCAGG GGTGAATATC 
AAGCCTGTGG TCAAAGTCAC GGCTGCCGGG CAGACCAAGA GGACACGGAT CCACAAGGGA 
AACAGCCCAC TCTTCAACGA GACTCTCTTC TTCAATGTGT TTGACTCTCC CTCGGAGCTG 
TTTGACGAGC CCATCTTTAT CACGGTGGTA GACTCCCGTT CTCTCCGGAC AGACGCTCTC 
ATTGGGGAAT TCCGGATGGA CGTGGGTACC ATCTACAGAG AGCCCCGACA CGCCTATCTG 
AGGAAATGGC TTCTCCTCTC GGACCCTGAC GATTTCTCTG CTGGGGCCAG AGGTTACCTG 
AAAGCAAGCC TCTGTGTGCT GGGGCCCGGC GATGAAGCGC CTCTGGAGAG AAAGGACCCC 
TCTGAAGACA AGGAGGACAT CGAAAACAAT CTGCTCAGGC CAACTGGCGT GGCCCTTCGA 
GGAGCCCACT TCTGCCTGAA GGTCTTCAGA GCTGAGGACT TACCACAGAT GGATGATGCC 
GTGATGGACA ACGTGAAGCA GATCTTCGGC TTTGACAGTA ACAAGAAGAA CTTGGTGGAT 
CCCTTCATGG AAGTCAGCTT TGCGGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAAACA 
GCCAACCCTC AGTGGAACCA GAGCATCACG CTGCCCGCCA TGTTTCCCTC CATGTGTGAA 
AAAATGAGGA TTCGCGTCAT GGACTGGGAC CGCCTCACCC ACAATGACAT CGTGGCCACC 
ACCTACCTGA GTATGTCGAA AATCTCCGCC CCTGGAGGAG AAATAGAAGT GGACGACCAT 
CTGGGCTTCC TCCCCACCTT CGGGCCCTGC TACGTCAACC TCTACGGCAG CCCCAGGGAG 
TTCACCGGCT TCCCGGACCC CTACGCAGAA CTCAACATGG GCAAGGGAGA AGGTGTGGCC 
TACCGAGGTC GGCTTCTGCT CTCCCTGGAG ACCAAGCTGG TGGAGCACAG CGACCAAAAG 
GTGGAGGACC TCCCTGCGGA TGACATCCTT CGGGTGGAGA AGTATCTCCG GAGGCGTAAG 
TACTCCCTCT TTGCCGCCTT CTACTCGGCC TCCATGCTCC AGGATGTGGA TGATGCCATC 
CAGTTCGAGG TCAGCATCGG GAACTATGGC AACAAGTTCG ACACCACGTG CCTGCCGCTG 
GCCTCTACCA CCCAGTACAG CCGGGCGGTC TTTGACGGGT GCCACTACTA CTACTTGCCC 
TGGGGCAACG TGAAGCCCGT GGTGGTGCTG TCATCCTACT GGGAGGACAT CAGCCACAGA 
GTTGAGGCTC AGAACCAGCT GCTCAGGATT GCGGACCGGC TGGAAGCTGC CCTGGAGCAA 
GTCCACTTGG CCGTGAAGGC GCAGTACAAC GCCGAGGACG TGGACTCCCT GTTGGCTCAG 
CTGATGGATG AGCTCATCTC AGACTGCAGC CAGCCCCTAG GCGATGTCCA CGAGACACCC 
TCTGCCACCC ACCTGGACCA GTACCTGCAC AGGCTGCGTA GCCGTCACCT AAGCCAGATT 
GCAGAGGCGG CGCTGGCCCT GAAGCTCGGG CACGGCGGGC TCCCGGCCGC CCTGGAGCAG 
GCTGAAGACT GGCTCCTGCG GCTCCGTGCC CTGGCCGAGG AGCCTCAGAA CAGCCTGCCC 
GACATCATCA TCTGGATGCT GCAGGGCGAC AAGCGAGTGG CATACCAGCG GGTGCCTACA 
CATGAGGTCC TCTTCTCCCG GCGTGGAGCC AGCTACTGCG GCAAGAACTG TGGGAAGCTG 
CAGACGATAT TTCTCAAATA TCCCATGCAG GGGGTGCCCG GAGCCCGGAT GCCCGTGCAG 
ATACGGGTGA AGCTCTGGTT TGGGCTTTCT GTGGATGAGA AGGAGTTCAA CCAGTTTGCT 
GAGGGAAAGC TGTCTGTCTT TGCTGAAACC TATGAGAACC AGACCAAACT GGCCCTCGTT 
GGGAACTGGG GCACAACAGG CCTTACCTAC CCCAAGTTTT CTGATGTCGC GGGCAAGATC 
AAGCTCCCCA AGGATAGCTT CCGCCCCTCA GCCGGCTGGA CCTGGGCTGG AGATTGGTTT 
GTGTGTCCAG AGAAGACGCT GCTCCATGAC ACCGACGCGG GCCACCTGAG TTTCGTGGCG 
GAGGTGTTTG AGAACCAGAC CCGGCTCCCT GGAGGCCACT GGGTCTACAT GAGTGACAAC 
TACACGGATG TGAATGGGGA GAAGGTGCTT CCCAAGGATG ACATTGAGTG CCCGCTGGGC 
TGGAAGTGGG AAGATGAAGA GTGGTCCACG GACCTCAATC GGGCCGTTGA TGAGCAAGGC 
TGGGAGTACA GTATCACCAT CCCCCCGGAC CGGAAGCCGA AGCACTGGGT CCCCGCTGAG 
AAGATGTACT ACACGCACCG TCGGCGGCGC TGGGTCCGCC TGCGCAGGAG GGACCTCAGC 
CAGATGGAGG CCCTGAGGAA GCACAGGCAG GCGGAGGCGG AGGGCGAGGG CTGGGAGTAC 
GCCTCGCTCT TTGGCTGGAA ATTCCACCTG GAGTACCGCA AAACGGATGC ATTCCGTCGC 
CGCCGCTGGC GCCGCCGCAT GGAGCCGCTG GAGAAGACGG GGCCTGCAGC CGTGTTTGCC 
CTTGAGGGGG CACTGGGTGG CGTGGTAGAT GACCGGAGTG AAGATTCCAT GTCCGTGTCC 
ACCTTGAGCT TTGGCGTGAA CAGACCCACG ATTTCCTGCA TCTTTGATCA TGGGAACCGC 
TACCACCTGC GCTGCTATAT GTACCAGGCT CGGGACCTGC CCTCGATGGA CAAGGACTCT 
TTTTCTGATC CCTACGCCAT CGTCTCCTTC CTGCACCAGA GCCAGAAGAC CGTGGTGGTG 
AAGAACACTC TGAACCCCAC CTGGGACCAG ACGCTCATCT TCTATGAGAT TGAGATCTTT 
GGAGAGCCAG CCAGCATCGC CGAGCAGCCG CCCAGCATTG TGGTGGAGCT GTATGACCAC 
GACACCTACG GTGCAGATGA GTTTTTGGGA CGCTGCATGT GTCAGCCCAG TCTGGAACGG 
ATGCCCCGGC TGGCCTGGTT CCCGCTGGTT AGGGGCAGCC AGCCGGCAGG CGAGCTGCTG 
GCCTCTTTTG AGCTCATCCA GAGAGAGAAG CCGGCCATTC ACCATATTCC TGGTTTTGAG 
GTGCAGGATA CATCGGGAAT CCTGGACGAG TCGGAGGACA CAGACCTGCC CTACCCACCG 
CCTCAGAGGG AGGCCAACAT CTACATGGTT CCCCAGAACA TCAAGCCGGT TCTCCAGCGC 
ACTGCCATAG AGATCCTGGC ATGGGGCCTA CGGAACATGA AGAGTTACCA GCTGGCCAGT 
ATCTCCTCCC CCAGCCTCGT GGTGGAGTGC GGGGGCCAGA CGGTGCAGTC CTGTGTCATC 
AGGAACCTCC GGAAGAACCC CAACTTCGAC ATCTGCACCC TCTTCATGGA AGTGATGCTG 
CCCAGAGAGG AGCTCTACTG TCCCCCCATC GTGATCAAGG TCATTGATAA CCGCCAGTTT 
GGCCGCCGGC CTGTGGTGGG CCAGTGTACC ATACGCTCCC TGGAGAGCTT CATGTGTGAC 
CTGTACTCCG AGGAGAGTCC GTCCCCACAG GGCGGCCCAG ATGATGTGAG TTTACTCAGT 
CCTGGGGAAG ACGTGCTCAT TGACATCGAC GACAAGGAGC CCCTCATCCC CATACAGCTT 
ACAGACGGTC TGTTGGGCTC TGCCCCCACT AACATGGCAT CCTCTCCATC CAGTCTTCAT 
GAGGAGGAGT TCATCGACTG GTGGAGCAAA TTCTTTGCCT CCACAGGGGA GAGGGAGAAG 
TGCGGCTCGT ACTTGGAGAA GGGCTTTGAC ACTCTAAAGG TCTATGACAC ACAACTGGAG 
AACGTGGAGG CCTTTGGGGG CCTGTCTGAC TTTTGTAACA CCTTCAAGCT CTACCGGGGC 
AAGACTCAGG AGGAGACGGA GGACCCATCT GTTATCGGGG AGTTTAAGGG CCTCTTCAAA 
ATCTACCCCC TCCCGGAAGA CCCGGCCGTC CCCATGCCCC CGAGACAGTT CCAGCAGCTG 
GCTGCCCAGG GGCCTCAGGA CTGCCTGGTC CGCATCTATA TCATCCGGGC ATTTGGCCTG 
CAGCCCAAGG ACCCCAATGG GAAGTGTGAT CCTTACATCA AGATCTCCAT AGGGAAGAAA 
TCAGTGAGTG ACCAGGATAA TTACATTCCC TGTACCCTGG AGCCTGTGTT TGGAAAGATG 
TTCGAGCTGA CCTGCACTCT GCCTCTGGAG AAGGACCTGA AGATCACACT CTACGACTAT 
GACCTCCTCT CCAAAGATGA GAAGATCGGG GAGACAGTCA TCGACCTGGA GAATAGGCTG 
CTGTCCAAGT TCGGGGCTCG CTGCGGACTC CCACAGACCT ACTGTGTCTC TGGACCCAAC 
CAGTGGAGGG ACCAGCTCCG CCCCTCCCAG CTTCTCCAGC TCACCTGCCA GCAGCGCAGA 
CTCAAGGCGC CCGTGTACCT CACAGACCGC GTACTCTTTC AGGATAAAGA ATACACCATT 
GAGGAAATAG AGGCTGGCAG GGTCCTGAAC CCACACCTGG GCCCAGTGGA AGAGCGTCTG 
GCCTTGCACG TCCTCCAGCA GCTGGGCCTG GTCCCCGAGC ATGTGGAGTC CCGGCCTCTT 
TACAGCCCCC TGCAGCCAGA CATTGAGCAG GGGAAGCTGC AGATGTGGAT CGATCTGTTT 
CCCAAGGCCC TGGGGCGACC TGGACCTCCC TTCAACATCA CCCCACGGAG AGCAAGAAGG 
TTTTTCCTGC GTTGTATTAT CTGGAACACA AAGGACGTGA TCCTGGACGA CCTCAGCATC 
ACGGGGGAGA AGATGAGTGA CATTTACGTG AAAGGTTGGA TGGTCGGCTT TGAAGAGCAC 
AAGCAGAAGA CGGACGTGCA CTACCGCTCC CTGGGAGGCG AAGGCAACTT TAACTGGAGG 
TTCATTTTCC CCTTTGACTA CCTGCCCGCT GAGCAAGTCT GCACCGTCTC TAAGAAGGAT 
GCTTTCTGGA GGCTGGACAA GACTGAGAGC AAAATCCCAG CACAAGTGGT ATTTCAGATC 
TGGGACAATG ACAAGTTCTC CTTTGACGAC TTTCTGGGCT CGTTGCAGTT GGATCTCAAC 
CGCATGCCCA AGCCTGCCAA GACAGCTGAG AAGTGCTCCT TGGACCAGCT GGACGACACT 
TTCCACCCGG AGTGGTTTGT GTCCCTTTTT GAACAGAAAA CAGTAAAGGG CTGGTGGCCC 
TGTGTGGCAG AAGACGGTGA GAAGAAGATC CTGGCGGGCA AGCTGGAAAT GACCTTGGAG 
ATCGTAGCAG AGAGTGAGCA CGAAGAGCGG CCTGCGGGTC AAGGCCGGGA TGAACCCAAC 
ATGAACCCCA AGCTCGAGGA CCCAAGGCGC CCCGACACCT CCTTCCTGTG GTTCACCTCT 
CCGTACAAGA CCATGAAGTT CATCCTGTGG CGGCGGTTCC GGTGCGCCAT CATCCTCTTC 
GTCGTTCTCG TCATCCTGCT GCTCTTCCTG GGCATCTTCC TCTATTCCTT CCCGAACTAT 
GCTGCCATGA AGCTGGTGAA ACCCTTCAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002914939.1
CDS	150..6422
Translation	MLSCLLVRASNLPSVKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEAQVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPSASLEPSPTLPDMDMVADTGGEEDTEDQGLTGDEAEPLLDPSGAPGPGAPTTPRKPPSHPPPYHPGSKRKRGAPTPRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVMDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDHLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRLLLSLETKLVEHSDQKVEDLPADDILRVEKYLRRRKYSLFAAFYSASMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADRLEAALEQVHLAVKAQYNAEDVDSLLAQLMDELISDCSQPLGDVHETPSATHLDQYLHRLRSRHLSQIAEAALALKLGHGGLPAALEQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPTHEVLFSRRGASYCGKNCGKLQTIFLKYPMQGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVAGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDTDAGHLSFVAEVFENQTRLPGGHWVYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALRKHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSMSVSTLSFGVNRPTISCIFDHGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFLGRCMCQPSLERMPRLAWFPLVRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTSGILDESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPIVIKVIDNRQFGRRPVVGQCTIRSLESFMCDLYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLTDGLLGSAPTNMASSPSSLHEEEFIDWWSKFFASTGEREKCGSYLEKGFDTLKVYDTQLENVEAFGGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAVPMPPRQFQQLAAQGPQDCLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLQLTCQQRRLKAPVYLTDRVLFQDKEYTIEEIEAGRVLNPHLGPVEERLALHVLQQLGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPAQVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEDGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFVVLVILLLFLGIFLYSFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_002914893.3
Organism	Ailuropoda melanoleuca(giant panda)
Definition	Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002914893.3

ORF Insert Sequence:

ATGCTGAGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGTGAAGAA GGACCGGCGC 
AGTGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCAGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTCCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCGG GAGGTCCTCG CCACCCCCAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCCTC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGTGGCTG ACACAGGAGG AGAGGAGGAC 
ACTGAGGACC AGGGGCTCAC GGGAGATGAG GCAGAGCCAT TACTGGATCC GAGCGGTGCC 
CCAGGCCCTG GGGCCCCCAC CACCCCGAGG AAGCCACCTT CCCATCCGCC CCCCTACCAC 
CCCGGGAGCA AGAGAAAAAG AGGTGCTCCC ACGCCCAGAA AGCTGCTGTC GGACAAACCG 
CAGGACTTCC AGATCAGGGT GCAAGTGATC GAGGGGCGCC AGCTGCCAGG GGTGAATATC 
AAGCCTGTGG TCAAAGTCAC GGCTGCCGGG CAGACCAAGA GGACACGGAT CCACAAGGGA 
AACAGCCCAC TCTTCAACGA GACTCTCTTC TTCAATGTGT TTGACTCTCC CTCGGAGCTG 
TTTGACGAGC CCATCTTTAT CACGGTGGTA GACTCCCGTT CTCTCCGGAC AGACGCTCTC 
ATTGGGGAAT TCCGGATGGA CGTGGGTACC ATCTACAGAG AGCCCCGACA CGCCTATCTG 
AGGAAATGGC TTCTCCTCTC GGACCCTGAC GATTTCTCTG CTGGGGCCAG AGGTTACCTG 
AAAGCAAGCC TCTGTGTGCT GGGGCCCGGC GATGAAGCGC CTCTGGAGAG AAAGGACCCC 
TCTGAAGACA AGGAGGACAT CGAAAACAAT CTGCTCAGGC CAACTGGCGT GGCCCTTCGA 
GGAGCCCACT TCTGCCTGAA GGTCTTCAGA GCTGAGGACT TACCACAGAT GGATGATGCC 
GTGATGGACA ACGTGAAGCA GATCTTCGGC TTTGACAGTA ACAAGAAGAA CTTGGTGGAT 
CCCTTCATGG AAGTCAGCTT TGCGGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAAACA 
GCCAACCCTC AGTGGAACCA GAGCATCACG CTGCCCGCCA TGTTTCCCTC CATGTGTGAA 
AAAATGAGGA TTCGCGTCAT GGACTGGGAC CGCCTCACCC ACAATGACAT CGTGGCCACC 
ACCTACCTGA GTATGTCGAA AATCTCCGCC CCTGGAGGAG AAATAGAAGT GGACGACCAT 
CTGGGCTTCC TCCCCACCTT CGGGCCCTGC TACGTCAACC TCTACGGCAG CCCCAGGGAG 
TTCACCGGCT TCCCGGACCC CTACGCAGAA CTCAACATGG GCAAGGGAGA AGGTGTGGCC 
TACCGAGGTC GGCTTCTGCT CTCCCTGGAG ACCAAGCTGG TGGAGCACAG CGACCAAAAG 
GTGGAGGACC TCCCTGCGGA TGACATCCTT CGGGTGGAGA AGTATCTCCG GAGGCGTAAG 
TACTCCCTCT TTGCCGCCTT CTACTCGGCC TCCATGCTCC AGGATGTGGA TGATGCCATC 
CAGTTCGAGG TCAGCATCGG GAACTATGGC AACAAGTTCG ACACCACGTG CCTGCCGCTG 
GCCTCTACCA CCCAGTACAG CCGGGCGGTC TTTGACGGGT GCCACTACTA CTACTTGCCC 
TGGGGCAACG TGAAGCCCGT GGTGGTGCTG TCATCCTACT GGGAGGACAT CAGCCACAGA 
GTTGAGGCTC AGAACCAGCT GCTCAGGATT GCGGACCGGC TGGAAGCTGC CCTGGAGCAA 
GTCCACTTGG CCGTGAAGGC GCAGTACAAC GCCGAGGACG TGGACTCCCT GTTGGCTCAG 
CTGATGGATG AGCTCATCTC AGACTGCAGC CAGCCCCTAG GCGATGTCCA CGAGACACCC 
TCTGCCACCC ACCTGGACCA GTACCTGCAC AGGCTGCGTA GCCGTCACCT AAGCCAGATT 
GCAGAGGCGG CGCTGGCCCT GAAGCTCGGG CACGGCGGGC TCCCGGCCGC CCTGGAGCAG 
GCTGAAGACT GGCTCCTGCG GCTCCGTGCC CTGGCCGAGG AGCCTCAGAA CAGCCTGCCC 
GACATCATCA TCTGGATGCT GCAGGGCGAC AAGCGAGTGG CATACCAGCG GGTGCCTACA 
CATGAGGTCC TCTTCTCCCG GCGTGGAGCC AGCTACTGCG GCAAGAACTG TGGGAAGCTG 
CAGACGATAT TTCTCAAATA TCCCATGCAG GGGGTGCCCG GAGCCCGGAT GCCCGTGCAG 
ATACGGGTGA AGCTCTGGTT TGGGCTTTCT GTGGATGAGA AGGAGTTCAA CCAGTTTGCT 
GAGGGAAAGC TGTCTGTCTT TGCTGAAACC TATGAGAACC AGACCAAACT GGCCCTCGTT 
GGGAACTGGG GCACAACAGG CCTTACCTAC CCCAAGTTTT CTGATGTCGC GGGCAAGATC 
AAGCTCCCCA AGGATAGCTT CCGCCCCTCA GCCGGCTGGA CCTGGGCTGG AGATTGGTTT 
GTGTGTCCAG AGAAGACGCT GCTCCATGAC ACCGACGCGG GCCACCTGAG TTTCGTGGCG 
GAGGTGTTTG AGAACCAGAC CCGGCTCCCT GGAGGCCACT GGGTCTACAT GAGTGACAAC 
TACACGGATG TGAATGGGGA GAAGGTGCTT CCCAAGGATG ACATTGAGTG CCCGCTGGGC 
TGGAAGTGGG AAGATGAAGA GTGGTCCACG GACCTCAATC GGGCCGTTGA TGAGCAAGGC 
TGGGAGTACA GTATCACCAT CCCCCCGGAC CGGAAGCCGA AGCACTGGGT CCCCGCTGAG 
AAGATGTACT ACACGCACCG TCGGCGGCGC TGGGTCCGCC TGCGCAGGAG GGACCTCAGC 
CAGATGGAGG CCCTGAGGAA GCACAGGCAG GCGGAGGCGG AGGGCGAGGG CTGGGAGTAC 
GCCTCGCTCT TTGGCTGGAA ATTCCACCTG GAGTACCGCA AAACGGATGC ATTCCGTCGC 
CGCCGCTGGC GCCGCCGCAT GGAGCCGCTG GAGAAGACGG GGCCTGCAGC CGTGTTTGCC 
CTTGAGGGGG CACTGGGTGG CGTGGTAGAT GACCGGAGTG AAGATTCCAT GTCCGTGTCC 
ACCTTGAGCT TTGGCGTGAA CAGACCCACG ATTTCCTGCA TCTTTGATCA TGGGAACCGC 
TACCACCTGC GCTGCTATAT GTACCAGGCT CGGGACCTGC CCTCGATGGA CAAGGACTCT 
TTTTCTGATC CCTACGCCAT CGTCTCCTTC CTGCACCAGA GCCAGAAGAC CGTGGTGGTG 
AAGAACACTC TGAACCCCAC CTGGGACCAG ACGCTCATCT TCTATGAGAT TGAGATCTTT 
GGAGAGCCAG CCAGCATCGC CGAGCAGCCG CCCAGCATTG TGGTGGAGCT GTATGACCAC 
GACACCTACG GTGCAGATGA GTTTTTGGGA CGCTGCATGT GTCAGCCCAG TCTGGAACGG 
ATGCCCCGGC TGGCCTGGTT CCCGCTGGTT AGGGGCAGCC AGCCGGCAGG CGAGCTGCTG 
GCCTCTTTTG AGCTCATCCA GAGAGAGAAG CCGGCCATTC ACCATATTCC TGGTTTTGAG 
GTGCAGGATA CATCGGGAAT CCTGGACGAG TCGGAGGACA CAGACCTGCC CTACCCACCG 
CCTCAGAGGG AGGCCAACAT CTACATGGTT CCCCAGAACA TCAAGCCGGT TCTCCAGCGC 
ACTGCCATAG AGATCCTGGC ATGGGGCCTA CGGAACATGA AGAGTTACCA GCTGGCCAGT 
ATCTCCTCCC CCAGCCTCGT GGTGGAGTGC GGGGGCCAGA CGGTGCAGTC CTGTGTCATC 
AGGAACCTCC GGAAGAACCC CAACTTCGAC ATCTGCACCC TCTTCATGGA AGTGATGCTG 
CCCAGAGAGG AGCTCTACTG TCCCCCCATC GTGATCAAGG TCATTGATAA CCGCCAGTTT 
GGCCGCCGGC CTGTGGTGGG CCAGTGTACC ATACGCTCCC TGGAGAGCTT CATGTGTGAC 
CTGTACTCCG AGGAGAGTCC GTCCCCACAG GGCGGCCCAG ATGATGTGAG TTTACTCAGT 
CCTGGGGAAG ACGTGCTCAT TGACATCGAC GACAAGGAGC CCCTCATCCC CATACAGCTT 
ACAGACGGTC TGTTGGGCTC TGCCCCCACT AACATGGCAT CCTCTCCATC CAGTCTTCAT 
GAGGAGGAGT TCATCGACTG GTGGAGCAAA TTCTTTGCCT CCACAGGGGA GAGGGAGAAG 
TGCGGCTCGT ACTTGGAGAA GGGCTTTGAC ACTCTAAAGG TCTATGACAC ACAACTGGAG 
AACGTGGAGG CCTTTGGGGG CCTGTCTGAC TTTTGTAACA CCTTCAAGCT CTACCGGGGC 
AAGACTCAGG AGGAGACGGA GGACCCATCT GTTATCGGGG AGTTTAAGGG CCTCTTCAAA 
ATCTACCCCC TCCCGGAAGA CCCGGCCGTC CCCATGCCCC CGAGACAGTT CCAGCAGCTG 
GCTGCCCAGG GGCCTCAGGA CTGCCTGGTC CGCATCTATA TCATCCGGGC ATTTGGCCTG 
CAGCCCAAGG ACCCCAATGG GAAGTGTGAT CCTTACATCA AGATCTCCAT AGGGAAGAAA 
TCAGTGAGTG ACCAGGATAA TTACATTCCC TGTACCCTGG AGCCTGTGTT TGGAAAGATG 
TTCGAGCTGA CCTGCACTCT GCCTCTGGAG AAGGACCTGA AGATCACACT CTACGACTAT 
GACCTCCTCT CCAAAGATGA GAAGATCGGG GAGACAGTCA TCGACCTGGA GAATAGGCTG 
CTGTCCAAGT TCGGGGCTCG CTGCGGACTC CCACAGACCT ACTGTGTCTC TGGACCCAAC 
CAGTGGAGGG ACCAGCTCCG CCCCTCCCAG CTTCTCCAGC TCACCTGCCA GCAGCGCAGA 
CTCAAGGCGC CCGTGTACCT CACAGACCGC GTACTCTTTC AGGATAAAGA ATACACCATT 
GAGGAAATAG AGGCTGGCAG GGTCCTGAAC CCACACCTGG GCCCAGTGGA AGAGCGTCTG 
GCCTTGCACG TCCTCCAGCA GCTGGGCCTG GTCCCCGAGC ATGTGGAGTC CCGGCCTCTT 
TACAGCCCCC TGCAGCCAGA CATTGAGCAG GGGAAGCTGC AGATGTGGAT CGATCTGTTT 
CCCAAGGCCC TGGGGCGACC TGGACCTCCC TTCAACATCA CCCCACGGAG AGCAAGAAGG 
TTTTTCCTGC GTTGTATTAT CTGGAACACA AAGGACGTGA TCCTGGACGA CCTCAGCATC 
ACGGGGGAGA AGATGAGTGA CATTTACGTG AAAGGTTGGA TGGTCGGCTT TGAAGAGCAC 
AAGCAGAAGA CGGACGTGCA CTACCGCTCC CTGGGAGGCG AAGGCAACTT TAACTGGAGG 
TTCATTTTCC CCTTTGACTA CCTGCCCGCT GAGCAAGTCT GCACCGTCTC TAAGAAGGAT 
GCTTTCTGGA GGCTGGACAA GACTGAGAGC AAAATCCCAG CACAAGTGGT ATTTCAGATC 
TGGGACAATG ACAAGTTCTC CTTTGACGAC TTTCTGGGCT CGTTGCAGTT GGATCTCAAC 
CGCATGCCCA AGCCTGCCAA GACAGCTGAG AAGTGCTCCT TGGACCAGCT GGACGACACT 
TTCCACCCGG AGTGGTTTGT GTCCCTTTTT GAACAGAAAA CAGTAAAGGG CTGGTGGCCC 
TGTGTGGCAG AAGACGGTGA GAAGAAGATC CTGGCGGGCA AGCTGGAAAT GACCTTGGAG 
ATCGTAGCAG AGAGTGAGCA CGAAGAGCGG CCTGCGGGTC AAGGCCGGGA TGAACCCAAC 
ATGAACCCCA AGCTCGAGGA CCCAAGGCGC CCCGACACCT CCTTCCTGTG GTTCACCTCT 
CCGTACAAGA CCATGAAGTT CATCCTGTGG CGGCGGTTCC GGTGCGCCAT CATCCTCTTC 
GTCGTTCTCG TCATCCTGCT GCTCTTCCTG GGCATCTTCC TCTATTCCTT CCCGAACTAT 
GCTGCCATGA AGCTGGTGAA ACCCTTCAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_002914894.3 ) cDNA ORF clone, Ailuropoda melanoleuca(giant panda) -> XP_002914940.1 Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X20, mRNA.

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CloneID	OAc02561
Clone ID Related Accession (Same CDS sequence)	XM_002914894.3
Accession Version	XM_002914894.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-27
Organism	Ailuropoda melanoleuca(giant panda)
Product	dysferlin isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003217356.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 28, 2016 this sequence version replaced XM_002914894.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ailuropoda melanoleuca Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGTGAAGAA GGACCGGCGC 
AGTGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCAGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTCCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCGG GAGGTCCTCG CCACCCCCAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCCTC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGTGGCTG ACACAGGAGG AGAGGAGGAC 
ACTGAGGACC AGGGGCTCAC GGGAGATGAG GCAGAGCCAT TACTGGATCC GAGCGGTGCC 
CCAGGCCCTG GGGCCCCCAC CACCCCGAGG AAGCCACCTT CCCATCCGCC CCCCTACCAC 
CCCGGGAGCA AGAGAAAAAG AGGTGCTCCC ACGCCCAGAA AGCTGCTGTC GGACAAACCG 
CAGGACTTCC AGATCAGGGT GCAAGTGATC GAGGGGCGCC AGCTGCCAGG GGTGAATATC 
AAGCCTGTGG TCAAAGTCAC GGCTGCCGGG CAGACCAAGA GGACACGGAT CCACAAGGGA 
AACAGCCCAC TCTTCAACGA GACTCTCTTC TTCAATGTGT TTGACTCTCC CTCGGAGCTG 
TTTGACGAGC CCATCTTTAT CACGGTGGTA GACTCCCGTT CTCTCCGGAC AGACGCTCTC 
ATTGGGGAAT TCCGGATGGA CGTGGGTACC ATCTACAGAG AGCCCCGACA CGCCTATCTG 
AGGAAATGGC TTCTCCTCTC GGACCCTGAC GATTTCTCTG CTGGGGCCAG AGGTTACCTG 
AAAGCAAGCC TCTGTGTGCT GGGGCCCGGC GATGAAGCGC CTCTGGAGAG AAAGGACCCC 
TCTGAAGACA AGGAGGACAT CGAAAACAAT CTGCTCAGGC CAACTGGCGT GGCCCTTCGA 
GGAGCCCACT TCTGCCTGAA GGTCTTCAGA GCTGAGGACT TACCACAGAT GGATGATGCC 
GTGATGGACA ACGTGAAGCA GATCTTCGGC TTTGACAGTA ACAAGAAGAA CTTGGTGGAT 
CCCTTCATGG AAGTCAGCTT TGCGGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAAACA 
GCCAACCCTC AGTGGAACCA GAGCATCACG CTGCCCGCCA TGTTTCCCTC CATGTGTGAA 
AAAATGAGGA TTCGCGTCAT GGACTGGGAC CGCCTCACCC ACAATGACAT CGTGGCCACC 
ACCTACCTGA GTATGTCGAA AATCTCCGCC CCTGGAGGAG AAATAGAAGT GGACGACCAT 
CTGGGCTTCC TCCCCACCTT CGGGCCCTGC TACGTCAACC TCTACGGCAG CCCCAGGGAG 
TTCACCGGCT TCCCGGACCC CTACGCAGAA CTCAACATGG GCAAGGGAGA AGGTGTGGCC 
TACCGAGGTC GGCTTCTGCT CTCCCTGGAG ACCAAGCTGG TGGAGCACAG CGACCAAAAG 
GTGGAGGACC TCCCTGCGGA TGACATCCTT CGGGTGGAGA AGTATCTCCG GAGGCGTAAG 
TACTCCCTCT TTGCCGCCTT CTACTCGGCC TCCATGCTCC AGGATGTGGA TGATGCCATC 
CAGTTCGAGG TCAGCATCGG GAACTATGGC AACAAGTTCG ACACCACGTG CCTGCCGCTG 
GCCTCTACCA CCCAGTACAG CCGGGCGGTC TTTGACGGGT GCCACTACTA CTACTTGCCC 
TGGGGCAACG TGAAGCCCGT GGTGGTGCTG TCATCCTACT GGGAGGACAT CAGCCACAGA 
GTTGAGGCTC AGAACCAGCT GCTCAGGATT GCGGACCGGC TGGAAGCTGC CCTGGAGCAA 
GTCCACTTGG CCGTGAAGGC GCAGTACAAC GCCGAGGACG TGGACTCCCT GTTGGCTCAG 
CTGATGGATG AGCTCATCTC AGACTGCAGC CAGCCCCTAG GCGATGTCCA CGAGACACCC 
TCTGCCACCC ACCTGGACCA GTACCTGCAC AGGCTGCGTA GCCGTCACCT AAGCCAGATT 
GCAGAGGCGG CGCTGGCCCT GAAGCTCGGG CACGGCGGGC TCCCGGCCGC CCTGGAGCAG 
GCTGAAGACT GGCTCCTGCG GCTCCGTGCC CTGGCCGAGG AGCCTCAGAA CAGCCTGCCC 
GACATCATCA TCTGGATGCT GCAGGGCGAC AAGCGAGTGG CATACCAGCG GGTGCCTACA 
CATGAGGTCC TCTTCTCCCG GCGTGGAGCC AGCTACTGCG GCAAGAACTG TGGGAAGCTG 
CAGACGATAT TTCTCAAATA TCCCATGCAG GGGGTGCCCG GAGCCCGGAT GCCCGTGCAG 
ATACGGGTGA AGCTCTGGTT TGGGCTTTCT GTGGATGAGA AGGAGTTCAA CCAGTTTGCT 
GAGGGAAAGC TGTCTGTCTT TGCTGAAACC TATGAGAACC AGACCAAACT GGCCCTCGTT 
GGGAACTGGG GCACAACAGG CCTTACCTAC CCCAAGTTTT CTGATGTCGC GGGCAAGATC 
AAGCTCCCCA AGGATAGCTT CCGCCCCTCA GCCGGCTGGA CCTGGGCTGG AGATTGGTTT 
GTGTGTCCAG AGAAGACGCT GCTCCATGAC ACCGACGCGG GCCACCTGAG TTTCGTGGCG 
GAGGTGTTTG AGAACCAGAC CCGGCTCCCT GGAGGCCACT GGGTCTACAT GAGTGACAAC 
TACACGGATG TGAATGGGGA GAAGGTGCTT CCCAAGGATG ACATTGAGTG CCCGCTGGGC 
TGGAAGTGGG AAGATGAAGA GTGGTCCACG GACCTCAATC GGGCCGTTGA TGAGCAAGGC 
TGGGAGTACA GTATCACCAT CCCCCCGGAC CGGAAGCCGA AGCACTGGGT CCCCGCTGAG 
AAGATGTACT ACACGCACCG TCGGCGGCGC TGGGTCCGCC TGCGCAGGAG GGACCTCAGC 
CAGATGGAGG CCCTGAGGAA GCACAGGCAG GCGGAGGCGG AGGGCGAGGG CTGGGAGTAC 
GCCTCGCTCT TTGGCTGGAA ATTCCACCTG GAGTACCGCA AAACGGATGC ATTCCGTCGC 
CGCCGCTGGC GCCGCCGCAT GGAGCCGCTG GAGAAGACGG GGCCTGCAGC CGTGTTTGCC 
CTTGAGGGGG CACTGGGTGG CGTGGTAGAT GACCGGAGTG AAGATTCCAT GTCCGTGTCC 
ACCTTGAGCT TTGGCGTGAA CAGACCCACG ATTTCCTGCA TCTTTGATCA TGGGAACCGC 
TACCACCTGC GCTGCTATAT GTACCAGGCT CGGGACCTGC CCTCGATGGA CAAGGACTCT 
TTTTCTGATC CCTACGCCAT CGTCTCCTTC CTGCACCAGA GCCAGAAGAC CGTGGTGGTG 
AAGAACACTC TGAACCCCAC CTGGGACCAG ACGCTCATCT TCTATGAGAT TGAGATCTTT 
GGAGAGCCAG CCAGCATCGC CGAGCAGCCG CCCAGCATTG TGGTGGAGCT GTATGACCAC 
GACACCTACG GTGCAGATGA GTTTTTGGGA CGCTGCATGT GTCAGCCCAG TCTGGAACGG 
ATGCCCCGGC TGGCCTGGTT CCCGCTGGTT AGGGGCAGCC AGCCGGCAGG CGAGCTGCTG 
GCCTCTTTTG AGCTCATCCA GAGAGAGAAG CCGGCCATTC ACCATATTCC TGGTTTTGAG 
GTGCAGGATA CATCGGGAAT CCTGGACGAG TCGGAGGACA CAGACCTGCC CTACCCACCG 
CCTCAGAGGG AGGCCAACAT CTACATGGTT CCCCAGAACA TCAAGCCGGT TCTCCAGCGC 
ACTGCCATAG AGATCCTGGC ATGGGGCCTA CGGAACATGA AGAGTTACCA GCTGGCCAGT 
ATCTCCTCCC CCAGCCTCGT GGTGGAGTGC GGGGGCCAGA CGGTGCAGTC CTGTGTCATC 
AGGAACCTCC GGAAGAACCC CAACTTCGAC ATCTGCACCC TCTTCATGGA AGTGATGCTG 
CCCAGAGAGG AGCTCTACTG TCCCCCCATC GTGATCAAGG TCATTGATAA CCGCCAGTTT 
GGCCGCCGGC CTGTGGTGGG CCAGTGTACC ATACGCTCCC TGGAGAGCTT CATGTGTGAC 
CTGTACTCCG AGGAGAGTCC GTCCCCACAG GGCGGCCCAG ATGATGTGAG TTTACTCAGT 
CCTGGGGAAG ACGTGCTCAT TGACATCGAC GACAAGGAGC CCCTCATCCC CATACAGGAG 
GAGGAGTTCA TCGACTGGTG GAGCAAATTC TTTGCCTCCA CAGGGGAGAG GGAGAAGTGC 
GGCTCGTACT TGGAGAAGGG CTTTGACACT CTAAAGGTCT ATGACACACA ACTGGAGAAC 
GTGGAGGCCT TTGGGGGCCT GTCTGACTTT TGTAACACCT TCAAGCTCTA CCGGGGCAAG 
ACTCAGGAGG AGACGGAGGA CCCATCTGTT ATCGGGGAGT TTAAGGGCCT CTTCAAAATC 
TACCCCCTCC CGGAAGACCC GGCCGTCCCC ATGCCCCCGA GACAGTTCCA GCAGCTGGCT 
GCCCAGGGGC CTCAGGACTG CCTGGTCCGC ATCTATATCA TCCGGGCATT TGGCCTGCAG 
CCCAAGGACC CCAATGGGAA GTGTGATCCT TACATCAAGA TCTCCATAGG GAAGAAATCA 
GTGAGTGACC AGGATAATTA CATTCCCTGT ACCCTGGAGC CTGTGTTTGG AAAGATGTTC 
GAGCTGACCT GCACTCTGCC TCTGGAGAAG GACCTGAAGA TCACACTCTA CGACTATGAC 
CTCCTCTCCA AAGATGAGAA GATCGGGGAG ACAGTCATCG ACCTGGAGAA TAGGCTGCTG 
TCCAAGTTCG GGGCTCGCTG CGGACTCCCA CAGACCTACT GTGTCTCTGG ACCCAACCAG 
TGGAGGGACC AGCTCCGCCC CTCCCAGCTT CTCCAGCTCA CCTGCCAGCA GCGCAGACTC 
AAGGCGCCCG TGTACCTCAC AGACCGCGTA CTCTTTCAGG ATAAAGAATA CACCATTGAG 
GAAATAGAGG CTGGCAGGGT CCTGAACCCA CACCTGGGCC CAGTGGAAGA GCGTCTGGCC 
TTGCACGTCC TCCAGCAGCT GGGCCTGGTC CCCGAGCATG TGGAGTCCCG GCCTCTTTAC 
AGCCCCCTGC AGCCAGACAT TGAGCAGGGG AAGCTGCAGA TGTGGATCGA TCTGTTTCCC 
AAGGCCCTGG GGCGACCTGG ACCTCCCTTC AACATCACCC CACGGAGAGC AAGAAGGTTT 
TTCCTGCGTT GTATTATCTG GAACACAAAG GACGTGATCC TGGACGACCT CAGCATCACG 
GGGGAGAAGA TGAGTGACAT TTACGTGAAA GGTTGGATGG TCGGCTTTGA AGAGCACAAG 
CAGAAGACGG ACGTGCACTA CCGCTCCCTG GGAGGCGAAG GCAACTTTAA CTGGAGGTTC 
ATTTTCCCCT TTGACTACCT GCCCGCTGAG CAAGTCTGCA CCGTCTCTAA GAAGGATGCT 
TTCTGGAGGC TGGACAAGAC TGAGAGCAAA ATCCCAGCAC AAGTGGTATT TCAGATCTGG 
GACAATGACA AGTTCTCCTT TGACGACTTT CTGGGCTCGT TGCAGTTGGA TCTCAACCGC 
ATGCCCAAGC CTGCCAAGAC AGCTGAGAAG TGCTCCTTGG ACCAGCTGGA CGACACTTTC 
CACCCGGAGT GGTTTGTGTC CCTTTTTGAA CAGAAAACAG TAAAGGGCTG GTGGCCCTGT 
GTGGCAGAAG ACGGTGAGAA GAAGATCCTG GCGGGCAAGC TGGAAATGAC CTTGGAGATC 
GTAGCAGAGA GTGAGCACGA AGAGCGGCCT GCGGGTCAAG GCCGGGATGA ACCCAACATG 
AACCCCAAGC TCGAGGACCC AAGGCGCCCC GACACCTCCT TCCTGTGGTT CACCTCTCCG 
TACAAGACCA TGAAGTTCAT CCTGTGGCGG CGGTTCCGGT GCGCCATCAT CCTCTTCGTC 
GTTCTCGTCA TCCTGCTGCT CTTCCTGGGC ATCTTCCTCT ATTCCTTCCC GAACTATGCT 
GCCATGAAGC TGGTGAAACC CTTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002914940.1
CDS	150..6359
Translation	MLSCLLVRASNLPSVKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEAQVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPSASLEPSPTLPDMDMVADTGGEEDTEDQGLTGDEAEPLLDPSGAPGPGAPTTPRKPPSHPPPYHPGSKRKRGAPTPRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVMDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDHLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRLLLSLETKLVEHSDQKVEDLPADDILRVEKYLRRRKYSLFAAFYSASMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADRLEAALEQVHLAVKAQYNAEDVDSLLAQLMDELISDCSQPLGDVHETPSATHLDQYLHRLRSRHLSQIAEAALALKLGHGGLPAALEQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPTHEVLFSRRGASYCGKNCGKLQTIFLKYPMQGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVAGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDTDAGHLSFVAEVFENQTRLPGGHWVYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALRKHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSMSVSTLSFGVNRPTISCIFDHGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFLGRCMCQPSLERMPRLAWFPLVRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTSGILDESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPIVIKVIDNRQFGRRPVVGQCTIRSLESFMCDLYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASTGEREKCGSYLEKGFDTLKVYDTQLENVEAFGGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAVPMPPRQFQQLAAQGPQDCLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLQLTCQQRRLKAPVYLTDRVLFQDKEYTIEEIEAGRVLNPHLGPVEERLALHVLQQLGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPAQVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEDGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFVVLVILLLFLGIFLYSFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_002914894.3
Organism	Ailuropoda melanoleuca(giant panda)
Definition	Ailuropoda melanoleuca dysferlin (DYSF), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002914894.3

ORF Insert Sequence:

ATGCTGAGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGTGAAGAA GGACCGGCGC 
AGTGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCAGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTCCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCGG GAGGTCCTCG CCACCCCCAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCCTC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGTGGCTG ACACAGGAGG AGAGGAGGAC 
ACTGAGGACC AGGGGCTCAC GGGAGATGAG GCAGAGCCAT TACTGGATCC GAGCGGTGCC 
CCAGGCCCTG GGGCCCCCAC CACCCCGAGG AAGCCACCTT CCCATCCGCC CCCCTACCAC 
CCCGGGAGCA AGAGAAAAAG AGGTGCTCCC ACGCCCAGAA AGCTGCTGTC GGACAAACCG 
CAGGACTTCC AGATCAGGGT GCAAGTGATC GAGGGGCGCC AGCTGCCAGG GGTGAATATC 
AAGCCTGTGG TCAAAGTCAC GGCTGCCGGG CAGACCAAGA GGACACGGAT CCACAAGGGA 
AACAGCCCAC TCTTCAACGA GACTCTCTTC TTCAATGTGT TTGACTCTCC CTCGGAGCTG 
TTTGACGAGC CCATCTTTAT CACGGTGGTA GACTCCCGTT CTCTCCGGAC AGACGCTCTC 
ATTGGGGAAT TCCGGATGGA CGTGGGTACC ATCTACAGAG AGCCCCGACA CGCCTATCTG 
AGGAAATGGC TTCTCCTCTC GGACCCTGAC GATTTCTCTG CTGGGGCCAG AGGTTACCTG 
AAAGCAAGCC TCTGTGTGCT GGGGCCCGGC GATGAAGCGC CTCTGGAGAG AAAGGACCCC 
TCTGAAGACA AGGAGGACAT CGAAAACAAT CTGCTCAGGC CAACTGGCGT GGCCCTTCGA 
GGAGCCCACT TCTGCCTGAA GGTCTTCAGA GCTGAGGACT TACCACAGAT GGATGATGCC 
GTGATGGACA ACGTGAAGCA GATCTTCGGC TTTGACAGTA ACAAGAAGAA CTTGGTGGAT 
CCCTTCATGG AAGTCAGCTT TGCGGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAAACA 
GCCAACCCTC AGTGGAACCA GAGCATCACG CTGCCCGCCA TGTTTCCCTC CATGTGTGAA 
AAAATGAGGA TTCGCGTCAT GGACTGGGAC CGCCTCACCC ACAATGACAT CGTGGCCACC 
ACCTACCTGA GTATGTCGAA AATCTCCGCC CCTGGAGGAG AAATAGAAGT GGACGACCAT 
CTGGGCTTCC TCCCCACCTT CGGGCCCTGC TACGTCAACC TCTACGGCAG CCCCAGGGAG 
TTCACCGGCT TCCCGGACCC CTACGCAGAA CTCAACATGG GCAAGGGAGA AGGTGTGGCC 
TACCGAGGTC GGCTTCTGCT CTCCCTGGAG ACCAAGCTGG TGGAGCACAG CGACCAAAAG 
GTGGAGGACC TCCCTGCGGA TGACATCCTT CGGGTGGAGA AGTATCTCCG GAGGCGTAAG 
TACTCCCTCT TTGCCGCCTT CTACTCGGCC TCCATGCTCC AGGATGTGGA TGATGCCATC 
CAGTTCGAGG TCAGCATCGG GAACTATGGC AACAAGTTCG ACACCACGTG CCTGCCGCTG 
GCCTCTACCA CCCAGTACAG CCGGGCGGTC TTTGACGGGT GCCACTACTA CTACTTGCCC 
TGGGGCAACG TGAAGCCCGT GGTGGTGCTG TCATCCTACT GGGAGGACAT CAGCCACAGA 
GTTGAGGCTC AGAACCAGCT GCTCAGGATT GCGGACCGGC TGGAAGCTGC CCTGGAGCAA 
GTCCACTTGG CCGTGAAGGC GCAGTACAAC GCCGAGGACG TGGACTCCCT GTTGGCTCAG 
CTGATGGATG AGCTCATCTC AGACTGCAGC CAGCCCCTAG GCGATGTCCA CGAGACACCC 
TCTGCCACCC ACCTGGACCA GTACCTGCAC AGGCTGCGTA GCCGTCACCT AAGCCAGATT 
GCAGAGGCGG CGCTGGCCCT GAAGCTCGGG CACGGCGGGC TCCCGGCCGC CCTGGAGCAG 
GCTGAAGACT GGCTCCTGCG GCTCCGTGCC CTGGCCGAGG AGCCTCAGAA CAGCCTGCCC 
GACATCATCA TCTGGATGCT GCAGGGCGAC AAGCGAGTGG CATACCAGCG GGTGCCTACA 
CATGAGGTCC TCTTCTCCCG GCGTGGAGCC AGCTACTGCG GCAAGAACTG TGGGAAGCTG 
CAGACGATAT TTCTCAAATA TCCCATGCAG GGGGTGCCCG GAGCCCGGAT GCCCGTGCAG 
ATACGGGTGA AGCTCTGGTT TGGGCTTTCT GTGGATGAGA AGGAGTTCAA CCAGTTTGCT 
GAGGGAAAGC TGTCTGTCTT TGCTGAAACC TATGAGAACC AGACCAAACT GGCCCTCGTT 
GGGAACTGGG GCACAACAGG CCTTACCTAC CCCAAGTTTT CTGATGTCGC GGGCAAGATC 
AAGCTCCCCA AGGATAGCTT CCGCCCCTCA GCCGGCTGGA CCTGGGCTGG AGATTGGTTT 
GTGTGTCCAG AGAAGACGCT GCTCCATGAC ACCGACGCGG GCCACCTGAG TTTCGTGGCG 
GAGGTGTTTG AGAACCAGAC CCGGCTCCCT GGAGGCCACT GGGTCTACAT GAGTGACAAC 
TACACGGATG TGAATGGGGA GAAGGTGCTT CCCAAGGATG ACATTGAGTG CCCGCTGGGC 
TGGAAGTGGG AAGATGAAGA GTGGTCCACG GACCTCAATC GGGCCGTTGA TGAGCAAGGC 
TGGGAGTACA GTATCACCAT CCCCCCGGAC CGGAAGCCGA AGCACTGGGT CCCCGCTGAG 
AAGATGTACT ACACGCACCG TCGGCGGCGC TGGGTCCGCC TGCGCAGGAG GGACCTCAGC 
CAGATGGAGG CCCTGAGGAA GCACAGGCAG GCGGAGGCGG AGGGCGAGGG CTGGGAGTAC 
GCCTCGCTCT TTGGCTGGAA ATTCCACCTG GAGTACCGCA AAACGGATGC ATTCCGTCGC 
CGCCGCTGGC GCCGCCGCAT GGAGCCGCTG GAGAAGACGG GGCCTGCAGC CGTGTTTGCC 
CTTGAGGGGG CACTGGGTGG CGTGGTAGAT GACCGGAGTG AAGATTCCAT GTCCGTGTCC 
ACCTTGAGCT TTGGCGTGAA CAGACCCACG ATTTCCTGCA TCTTTGATCA TGGGAACCGC 
TACCACCTGC GCTGCTATAT GTACCAGGCT CGGGACCTGC CCTCGATGGA CAAGGACTCT 
TTTTCTGATC CCTACGCCAT CGTCTCCTTC CTGCACCAGA GCCAGAAGAC CGTGGTGGTG 
AAGAACACTC TGAACCCCAC CTGGGACCAG ACGCTCATCT TCTATGAGAT TGAGATCTTT 
GGAGAGCCAG CCAGCATCGC CGAGCAGCCG CCCAGCATTG TGGTGGAGCT GTATGACCAC 
GACACCTACG GTGCAGATGA GTTTTTGGGA CGCTGCATGT GTCAGCCCAG TCTGGAACGG 
ATGCCCCGGC TGGCCTGGTT CCCGCTGGTT AGGGGCAGCC AGCCGGCAGG CGAGCTGCTG 
GCCTCTTTTG AGCTCATCCA GAGAGAGAAG CCGGCCATTC ACCATATTCC TGGTTTTGAG 
GTGCAGGATA CATCGGGAAT CCTGGACGAG TCGGAGGACA CAGACCTGCC CTACCCACCG 
CCTCAGAGGG AGGCCAACAT CTACATGGTT CCCCAGAACA TCAAGCCGGT TCTCCAGCGC 
ACTGCCATAG AGATCCTGGC ATGGGGCCTA CGGAACATGA AGAGTTACCA GCTGGCCAGT 
ATCTCCTCCC CCAGCCTCGT GGTGGAGTGC GGGGGCCAGA CGGTGCAGTC CTGTGTCATC 
AGGAACCTCC GGAAGAACCC CAACTTCGAC ATCTGCACCC TCTTCATGGA AGTGATGCTG 
CCCAGAGAGG AGCTCTACTG TCCCCCCATC GTGATCAAGG TCATTGATAA CCGCCAGTTT 
GGCCGCCGGC CTGTGGTGGG CCAGTGTACC ATACGCTCCC TGGAGAGCTT CATGTGTGAC 
CTGTACTCCG AGGAGAGTCC GTCCCCACAG GGCGGCCCAG ATGATGTGAG TTTACTCAGT 
CCTGGGGAAG ACGTGCTCAT TGACATCGAC GACAAGGAGC CCCTCATCCC CATACAGGAG 
GAGGAGTTCA TCGACTGGTG GAGCAAATTC TTTGCCTCCA CAGGGGAGAG GGAGAAGTGC 
GGCTCGTACT TGGAGAAGGG CTTTGACACT CTAAAGGTCT ATGACACACA ACTGGAGAAC 
GTGGAGGCCT TTGGGGGCCT GTCTGACTTT TGTAACACCT TCAAGCTCTA CCGGGGCAAG 
ACTCAGGAGG AGACGGAGGA CCCATCTGTT ATCGGGGAGT TTAAGGGCCT CTTCAAAATC 
TACCCCCTCC CGGAAGACCC GGCCGTCCCC ATGCCCCCGA GACAGTTCCA GCAGCTGGCT 
GCCCAGGGGC CTCAGGACTG CCTGGTCCGC ATCTATATCA TCCGGGCATT TGGCCTGCAG 
CCCAAGGACC CCAATGGGAA GTGTGATCCT TACATCAAGA TCTCCATAGG GAAGAAATCA 
GTGAGTGACC AGGATAATTA CATTCCCTGT ACCCTGGAGC CTGTGTTTGG AAAGATGTTC 
GAGCTGACCT GCACTCTGCC TCTGGAGAAG GACCTGAAGA TCACACTCTA CGACTATGAC 
CTCCTCTCCA AAGATGAGAA GATCGGGGAG ACAGTCATCG ACCTGGAGAA TAGGCTGCTG 
TCCAAGTTCG GGGCTCGCTG CGGACTCCCA CAGACCTACT GTGTCTCTGG ACCCAACCAG 
TGGAGGGACC AGCTCCGCCC CTCCCAGCTT CTCCAGCTCA CCTGCCAGCA GCGCAGACTC 
AAGGCGCCCG TGTACCTCAC AGACCGCGTA CTCTTTCAGG ATAAAGAATA CACCATTGAG 
GAAATAGAGG CTGGCAGGGT CCTGAACCCA CACCTGGGCC CAGTGGAAGA GCGTCTGGCC 
TTGCACGTCC TCCAGCAGCT GGGCCTGGTC CCCGAGCATG TGGAGTCCCG GCCTCTTTAC 
AGCCCCCTGC AGCCAGACAT TGAGCAGGGG AAGCTGCAGA TGTGGATCGA TCTGTTTCCC 
AAGGCCCTGG GGCGACCTGG ACCTCCCTTC AACATCACCC CACGGAGAGC AAGAAGGTTT 
TTCCTGCGTT GTATTATCTG GAACACAAAG GACGTGATCC TGGACGACCT CAGCATCACG 
GGGGAGAAGA TGAGTGACAT TTACGTGAAA GGTTGGATGG TCGGCTTTGA AGAGCACAAG 
CAGAAGACGG ACGTGCACTA CCGCTCCCTG GGAGGCGAAG GCAACTTTAA CTGGAGGTTC 
ATTTTCCCCT TTGACTACCT GCCCGCTGAG CAAGTCTGCA CCGTCTCTAA GAAGGATGCT 
TTCTGGAGGC TGGACAAGAC TGAGAGCAAA ATCCCAGCAC AAGTGGTATT TCAGATCTGG 
GACAATGACA AGTTCTCCTT TGACGACTTT CTGGGCTCGT TGCAGTTGGA TCTCAACCGC 
ATGCCCAAGC CTGCCAAGAC AGCTGAGAAG TGCTCCTTGG ACCAGCTGGA CGACACTTTC 
CACCCGGAGT GGTTTGTGTC CCTTTTTGAA CAGAAAACAG TAAAGGGCTG GTGGCCCTGT 
GTGGCAGAAG ACGGTGAGAA GAAGATCCTG GCGGGCAAGC TGGAAATGAC CTTGGAGATC 
GTAGCAGAGA GTGAGCACGA AGAGCGGCCT GCGGGTCAAG GCCGGGATGA ACCCAACATG 
AACCCCAAGC TCGAGGACCC AAGGCGCCCC GACACCTCCT TCCTGTGGTT CACCTCTCCG 
TACAAGACCA TGAAGTTCAT CCTGTGGCGG CGGTTCCGGT GCGCCATCAT CCTCTTCGTC 
GTTCTCGTCA TCCTGCTGCT CTTCCTGGGC ATCTTCCTCT ATTCCTTCCC GAACTATGCT 
GCCATGAAGC TGGTGAAACC CTTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	DYSF
Entrez Gene ID	100479469
Full Name	dysferlin
Gene Type	protein-coding
Organism	Ailuropoda melanoleuca(giant panda)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

DYSF cDNA ORF clone, Ailuropoda melanoleuca(giant panda)

Gene

Clones

gRNAs

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