H257_13996 cDNA ORF clone, Aphanomyces astaci

Gene
Clones
gRNAs

Summary

Gene Symbol	H257_13996
Entrez Gene ID	20815992
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Aphanomyces astaci

mRNA and Protein(s)

mRNA	Protein	Name
XM_009841709.1	XP_009840011.1	hypothetical protein
XM_009841710.1	XP_009840012.1	hypothetical protein, variant 1
XM_009841708.1	XP_009840010.1	hypothetical protein, variant 2

Pathways from BioSystems
Gene Ontology

The following H257_13996 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the H257_13996 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAp113533	XM_009841708.1 Latest version!	Aphanomyces astaci hypothetical protein, variant 2 mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OAp113535	XM_009841710.1 Latest version!	Aphanomyces astaci hypothetical protein, variant 1 mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OAp113534	XM_009841709.1 Latest version!	Aphanomyces astaci hypothetical protein mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

H257_13996 ( XM_009841708.1 ) cDNA ORF clone, Aphanomyces astaci -> XP_009840010.1 Aphanomyces astaci hypothetical protein, variant 2 mRNA.

Price & Availability↑

CloneID	OAp113533
Clone ID Related Accession (Same CDS sequence)	XM_009841708.1
Accession Version	XM_009841708.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3633bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-20
Organism	Aphanomyces astaci
Product	hypothetical protein, variant 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_009798315).

ATGATGATGG CGAGGGTACA TCAGGCGATG CTGCAATTGG GGGCCAACGC CAACATGGCT 
ACCAGAATAC ATGAGCCGCG CCCACCGAGA TGTCGACGCC GCTCCCAATC TAAAATGTCT 
ACTACCTTGA ATACCTCTCA GCCCAACCGC CTACGAGAAG CTATGGAAAC AATGTCGCTA 
CATGACGTGG CGCAACACAC AGTCGAGCTC AAGACCTTGA AGCTGCTGGT ACCTCGACCC 
CTATCATCCC CGCAGCTACA ATCATCTACT GCAACATCCT GCGACACGGT GGAACCGATA 
GCGTCCCCTC TAGCCATTGC ATTGGTCGCT CGATCATACG ACATTGCCGA CCTGTTGTTG 
CAATATGGCG CCAACATCAT CGTGCGAGAC CAAGTATGGA GAGCTTATGT GGACGATGCC 
AACAGCCGTG CGACGCTAGT CCAGTGGAGT TCACGCTGGC TGTGTCCAGA TATCTTTCCT 
TCAAGTGCTT TCGCCACACT GTGTGGCCAG GCTTCGTTTA CTCCCAGTAT CTTTCCCCGT 
AATGACACCA ACGACAACTC AGACGCAAAA CATGCGATGA CGGTTATCGA ATCGGGACTT 
TGTGCTTTGT TGGATGTGTC AATTGGGCTG AATTATTACT CCACTTGGGA CAATCTAGCC 
CACGCTGCAT TCTCGGCGTT TCGCCTACGA AGACTGCACT TGGCCCATCG CCTAGCCAAC 
CTATGCGGCG CTACGTTGTT TAACGATTCG CGCTCGACAA GTTGGCTAGT GGTAGCTGTG 
GAGAATGCAT GTCTCGCCAG CTGCCGGTGG CTTCAAGCTC ATGACTACCC CTTCTCAGCT 
ATCGACATCC CGGCCATGTG TCTACTGCTG AGCTCTCCCT GTCGACACCG ATCCGTTAAC 
GACGACATTG CCGTTCAAAC TCGGACGAGT CTGTTTGTAT GGCTCGTACA GTGCCATGTC 
AGCCTGATTC AGTCCTCCTA CCTTCAAACG TATTTGGACC TGGCGTTGGC CAATGCAAAC 
ACCGCTGTGA CCCGACTATT GGTGGATAAC ATGATTCACA CCGAGGACAA GAGCTACTAC 
ACCATTCGTG CGGACCACGC TGTGGTAGCC AGTCGTGGAG ATGAAACATA TATCCTGTCG 
CTTCGTTCGA TGGGATGCTG GACACATTGC GACCTTGGGC TGACGCTGGC CATCCATTTC 
AACATGTCGC AGACGATCCT GCGCTGTATT ATTGCGCACG GCGCCCGACC TACCCCTCAA 
GCCGTCGTCT ACGGCAAACC CGCCATCGTG TGGGCCATCT GGCATCGGCG CCTTGACATT 
GTTCGGGTCC TTTGTATCTT GGAGCCCACA CTCTGTCTTG CGACGACGGA TGGTCAAGGA 
CGAGATGCAT TGTATTATGC CATGCGAACC AACGATGTGT CCGTGGTGGC GTTTCTATGG 
ACGAACCGAC GACAGAGCCA GTTCAACTTT GCCGCCGCAT TGGTGGCTGC AGTGGAATCC 
AATGCTATGG CGTCCCTGAA ATGGATGGCT GCCACGGCAC CACAAGATTT TTTTCGGTCT 
ATCGAGGCGC TACACGTGCA CATGCCACAT GTGACATCGT TGGAGCATGT AGCTTGTGCG 
CGAGGGTTTC TCGAGCTTGC GACGTGGCTA CGCTCGATAA ACAGCAACCC CACGACGACA 
TGCTTGTCAT CGTCAAATGC TGATGGGTGC ACACCTACGC AGGTCGCAAG GTTGTTTGGG 
TATCCAATAC ATTTCGACGA ATCCAAGGGG GATAACTGCT GCCATGACCC TGTGGTGTTG 
AATGGTGTGC TGCGACGGCT CTTGTCCACA CAAGTGTCGG TCATCTCTAC GGGGAACAAG 
TCTATCGATG GCACTGCTGC TTGGCGGCCA CTATATCCAA TTGCAAGCCA AGCCTTTCAA 
TTCACCGAGC TATACGCAGC GTGTAGCAAC AACTCTGTCG CATACGTCAG CATCTTGGCC 
TCGTTCGGAG TGTCCCTCGT CGAGCCCTGT GGACCACTCA ACCTGCCACC GTTGGATTGG 
GCAGCTCACT ATGGAGCCGT CGACGTGATC CAGTGCCTGC TTAGCCAAGG CGTGGTGGAC 
GTAGAAGGGA CGGCGCTGAG GCGGGCGGCT ACCCGACGCC AGGGGTCAGC AGTTGCGGCT 
TTGCTGGATG CAGCTCCGCC TGCCACGTCA TTGGGGCGAC TGCAAGGGGA TGGGGCTACA 
CCCACACTGC TGCATTTAGT AGCTCGTGTC CAATCAACTG TTGCCCCGCG CTTGATCCAA 
GTTTTGATAG ACCAGTACCA CGCGGATGTG GATATTGTGG ATGGCCAAGG GTTGACACCC 
GTTGTGTATG CGATGGTGAG TGGCAACGTG CCGGCGATGC TTTGTTTGTT GGAGCGAGGT 
GCACGGCTGG AGGCCGAATA CGAAGGCCAA TCCGGATTCT ACTATGTCCT GCACCTACTC 
CCCTCGGACA CGTGGCGTGT CTTCTTTCAA GCGTATTTGG GTGTGGTACT ACACGGCCGA 
GCACTGCACT GCACAGAATC GTGCGGATGC AAGTCGTTTG AAGGGTCGGT AGGTGACGTG 
GCGTGTTCGT TTTGCAGCCA CCGATTTGAC CAACATTCCC ACATCCCCCT CCCCCCCTGG 
CATCAAGACG TGTCCGATAC GTACCGCATG CAACGTACTA GTAGTACTAC TAGTAGTCAT 
ATATCCCCCG AATCAAGCCC CCGATCCACA ACATCCGACA ACGACGACCA CGATAATGTC 
CATCGTTTCC AACACGATCG TTCCGATTTC GAGAATCAAG ACCCCCATCT ATCTCCGCTG 
CCATTGTCCA CACTAGAGTA TGACTACGTC GGTCGGTTGG ATCAATGTCA TATTTCTGCG 
GTCGCCTGGG CACGATTCCG CCCCGAAGTC TCGATGTATT GTCTGTCGTC GATGACTGGT 
CATCCGGATG CAGGTCCATC AAGCACCACC GACGACGACG ACGAAGACAA AGAGGATGTG 
TTGCAACGCG GGTTTGTGTG TTCATTGGAC GATCAACCAG AGGCGTGGTC ATGGAGGGTT 
CCGGCATTGA ATCAATCTGA CATCCCGGTG AAGATGGACA AACAACTAAT TCCCCCGAGG 
TGGTCTGTGG CTACTACGTT CGGACTAGAG TATCACGTCG ACTGCTGGTG TACACAAGGG 
GGCATGTACT GTCTGTCGGC CGCCATGAGT TTACGACGAG TGGTGACTTG GTGGCTAGGG 
AGACGCCTTC GATCTACGGG ATCTGCGACG ACGACAACGA AGACCGTCAC AACGTTGTTT 
CACATATGGA AATCCGACGG TCGTCGTCGT ATTTCGTCTC CGCGCGACCA CGTGGTGCCC 
CGTACGCGCC AGCAACTAGT GCGACTCAAG CTTTTTGTCT TGCACTGGTG CCACGCCAAG 
CTCGTTCAAG CATTTAGCCG GTGGAAAAAG GTGCCAACAG CATCATACAC CATCCATCTG 
CGACACCATA CAGAGGTGGT GGTGGTGGCT GCTCCGCCGC AAGCCTCCAA TATGGGGGAT 
CACAGTTACT GTAATTCCCT GAACAAACTC AATCCACACA AGCCCGTGCC TAGACTAAAA 
AACTCGACAC TTTCAACAGT GGATTGTGGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009840010.1
CDS	14..3646
Translation	MMMARVHQAMLQLGANANMATRIHEPRPPRCRRRSQSKMSTTLNTSQPNRLREAMETMSLHDVAQHTVELKTLKLLVPRPLSSPQLQSSTATSCDTVEPIASPLAIALVARSYDIADLLLQYGANIIVRDQVWRAYVDDANSRATLVQWSSRWLCPDIFPSSAFATLCGQASFTPSIFPRNDTNDNSDAKHAMTVIESGLCALLDVSIGLNYYSTWDNLAHAAFSAFRLRRLHLAHRLANLCGATLFNDSRSTSWLVVAVENACLASCRWLQAHDYPFSAIDIPAMCLLLSSPCRHRSVNDDIAVQTRTSLFVWLVQCHVSLIQSSYLQTYLDLALANANTAVTRLLVDNMIHTEDKSYYTIRADHAVVASRGDETYILSLRSMGCWTHCDLGLTLAIHFNMSQTILRCIIAHGARPTPQAVVYGKPAIVWAIWHRRLDIVRVLCILEPTLCLATTDGQGRDALYYAMRTNDVSVVAFLWTNRRQSQFNFAAALVAAVESNAMASLKWMAATAPQDFFRSIEALHVHMPHVTSLEHVACARGFLELATWLRSINSNPTTTCLSSSNADGCTPTQVARLFGYPIHFDESKGDNCCHDPVVLNGVLRRLLSTQVSVISTGNKSIDGTAAWRPLYPIASQAFQFTELYAACSNNSVAYVSILASFGVSLVEPCGPLNLPPLDWAAHYGAVDVIQCLLSQGVVDVEGTALRRAATRRQGSAVAALLDAAPPATSLGRLQGDGATPTLLHLVARVQSTVAPRLIQVLIDQYHADVDIVDGQGLTPVVYAMVSGNVPAMLCLLERGARLEAEYEGQSGFYYVLHLLPSDTWRVFFQAYLGVVLHGRALHCTESCGCKSFEGSVGDVACSFCSHRFDQHSHIPLPPWHQDVSDTYRMQRTSSTTSSHISPESSPRSTTSDNDDHDNVHRFQHDRSDFENQDPHLSPLPLSTLEYDYVGRLDQCHISAVAWARFRPEVSMYCLSSMTGHPDAGPSSTTDDDDEDKEDVLQRGFVCSLDDQPEAWSWRVPALNQSDIPVKMDKQLIPPRWSVATTFGLEYHVDCWCTQGGMYCLSAAMSLRRVVTWWLGRRLRSTGSATTTTKTVTTLFHIWKSDGRRRISSPRDHVVPRTRQQLVRLKLFVLHWCHAKLVQAFSRWKKVPTASYTIHLRHHTEVVVVAAPPQASNMGDHSYCNSLNKLNPHKPVPRLKNSTLSTVDCG

Target ORF information:

RefSeq Version	XM_009841708.1
Organism	Aphanomyces astaci
Definition	Aphanomyces astaci hypothetical protein, variant 2 mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009841708.1

ORF Insert Sequence:

ATGATGATGG CGAGGGTACA TCAGGCGATG CTGCAATTGG GGGCCAACGC CAACATGGCT 
ACCAGAATAC ATGAGCCGCG CCCACCGAGA TGTCGACGCC GCTCCCAATC TAAAATGTCT 
ACTACCTTGA ATACCTCTCA GCCCAACCGC CTACGAGAAG CTATGGAAAC AATGTCGCTA 
CATGACGTGG CGCAACACAC AGTCGAGCTC AAGACCTTGA AGCTGCTGGT ACCTCGACCC 
CTATCATCCC CGCAGCTACA ATCATCTACT GCAACATCCT GCGACACGGT GGAACCGATA 
GCGTCCCCTC TAGCCATTGC ATTGGTCGCT CGATCATACG ACATTGCCGA CCTGTTGTTG 
CAATATGGCG CCAACATCAT CGTGCGAGAC CAAGTATGGA GAGCTTATGT GGACGATGCC 
AACAGCCGTG CGACGCTAGT CCAGTGGAGT TCACGCTGGC TGTGTCCAGA TATCTTTCCT 
TCAAGTGCTT TCGCCACACT GTGTGGCCAG GCTTCGTTTA CTCCCAGTAT CTTTCCCCGT 
AATGACACCA ACGACAACTC AGACGCAAAA CATGCGATGA CGGTTATCGA ATCGGGACTT 
TGTGCTTTGT TGGATGTGTC AATTGGGCTG AATTATTACT CCACTTGGGA CAATCTAGCC 
CACGCTGCAT TCTCGGCGTT TCGCCTACGA AGACTGCACT TGGCCCATCG CCTAGCCAAC 
CTATGCGGCG CTACGTTGTT TAACGATTCG CGCTCGACAA GTTGGCTAGT GGTAGCTGTG 
GAGAATGCAT GTCTCGCCAG CTGCCGGTGG CTTCAAGCTC ATGACTACCC CTTCTCAGCT 
ATCGACATCC CGGCCATGTG TCTACTGCTG AGCTCTCCCT GTCGACACCG ATCCGTTAAC 
GACGACATTG CCGTTCAAAC TCGGACGAGT CTGTTTGTAT GGCTCGTACA GTGCCATGTC 
AGCCTGATTC AGTCCTCCTA CCTTCAAACG TATTTGGACC TGGCGTTGGC CAATGCAAAC 
ACCGCTGTGA CCCGACTATT GGTGGATAAC ATGATTCACA CCGAGGACAA GAGCTACTAC 
ACCATTCGTG CGGACCACGC TGTGGTAGCC AGTCGTGGAG ATGAAACATA TATCCTGTCG 
CTTCGTTCGA TGGGATGCTG GACACATTGC GACCTTGGGC TGACGCTGGC CATCCATTTC 
AACATGTCGC AGACGATCCT GCGCTGTATT ATTGCGCACG GCGCCCGACC TACCCCTCAA 
GCCGTCGTCT ACGGCAAACC CGCCATCGTG TGGGCCATCT GGCATCGGCG CCTTGACATT 
GTTCGGGTCC TTTGTATCTT GGAGCCCACA CTCTGTCTTG CGACGACGGA TGGTCAAGGA 
CGAGATGCAT TGTATTATGC CATGCGAACC AACGATGTGT CCGTGGTGGC GTTTCTATGG 
ACGAACCGAC GACAGAGCCA GTTCAACTTT GCCGCCGCAT TGGTGGCTGC AGTGGAATCC 
AATGCTATGG CGTCCCTGAA ATGGATGGCT GCCACGGCAC CACAAGATTT TTTTCGGTCT 
ATCGAGGCGC TACACGTGCA CATGCCACAT GTGACATCGT TGGAGCATGT AGCTTGTGCG 
CGAGGGTTTC TCGAGCTTGC GACGTGGCTA CGCTCGATAA ACAGCAACCC CACGACGACA 
TGCTTGTCAT CGTCAAATGC TGATGGGTGC ACACCTACGC AGGTCGCAAG GTTGTTTGGG 
TATCCAATAC ATTTCGACGA ATCCAAGGGG GATAACTGCT GCCATGACCC TGTGGTGTTG 
AATGGTGTGC TGCGACGGCT CTTGTCCACA CAAGTGTCGG TCATCTCTAC GGGGAACAAG 
TCTATCGATG GCACTGCTGC TTGGCGGCCA CTATATCCAA TTGCAAGCCA AGCCTTTCAA 
TTCACCGAGC TATACGCAGC GTGTAGCAAC AACTCTGTCG CATACGTCAG CATCTTGGCC 
TCGTTCGGAG TGTCCCTCGT CGAGCCCTGT GGACCACTCA ACCTGCCACC GTTGGATTGG 
GCAGCTCACT ATGGAGCCGT CGACGTGATC CAGTGCCTGC TTAGCCAAGG CGTGGTGGAC 
GTAGAAGGGA CGGCGCTGAG GCGGGCGGCT ACCCGACGCC AGGGGTCAGC AGTTGCGGCT 
TTGCTGGATG CAGCTCCGCC TGCCACGTCA TTGGGGCGAC TGCAAGGGGA TGGGGCTACA 
CCCACACTGC TGCATTTAGT AGCTCGTGTC CAATCAACTG TTGCCCCGCG CTTGATCCAA 
GTTTTGATAG ACCAGTACCA CGCGGATGTG GATATTGTGG ATGGCCAAGG GTTGACACCC 
GTTGTGTATG CGATGGTGAG TGGCAACGTG CCGGCGATGC TTTGTTTGTT GGAGCGAGGT 
GCACGGCTGG AGGCCGAATA CGAAGGCCAA TCCGGATTCT ACTATGTCCT GCACCTACTC 
CCCTCGGACA CGTGGCGTGT CTTCTTTCAA GCGTATTTGG GTGTGGTACT ACACGGCCGA 
GCACTGCACT GCACAGAATC GTGCGGATGC AAGTCGTTTG AAGGGTCGGT AGGTGACGTG 
GCGTGTTCGT TTTGCAGCCA CCGATTTGAC CAACATTCCC ACATCCCCCT CCCCCCCTGG 
CATCAAGACG TGTCCGATAC GTACCGCATG CAACGTACTA GTAGTACTAC TAGTAGTCAT 
ATATCCCCCG AATCAAGCCC CCGATCCACA ACATCCGACA ACGACGACCA CGATAATGTC 
CATCGTTTCC AACACGATCG TTCCGATTTC GAGAATCAAG ACCCCCATCT ATCTCCGCTG 
CCATTGTCCA CACTAGAGTA TGACTACGTC GGTCGGTTGG ATCAATGTCA TATTTCTGCG 
GTCGCCTGGG CACGATTCCG CCCCGAAGTC TCGATGTATT GTCTGTCGTC GATGACTGGT 
CATCCGGATG CAGGTCCATC AAGCACCACC GACGACGACG ACGAAGACAA AGAGGATGTG 
TTGCAACGCG GGTTTGTGTG TTCATTGGAC GATCAACCAG AGGCGTGGTC ATGGAGGGTT 
CCGGCATTGA ATCAATCTGA CATCCCGGTG AAGATGGACA AACAACTAAT TCCCCCGAGG 
TGGTCTGTGG CTACTACGTT CGGACTAGAG TATCACGTCG ACTGCTGGTG TACACAAGGG 
GGCATGTACT GTCTGTCGGC CGCCATGAGT TTACGACGAG TGGTGACTTG GTGGCTAGGG 
AGACGCCTTC GATCTACGGG ATCTGCGACG ACGACAACGA AGACCGTCAC AACGTTGTTT 
CACATATGGA AATCCGACGG TCGTCGTCGT ATTTCGTCTC CGCGCGACCA CGTGGTGCCC 
CGTACGCGCC AGCAACTAGT GCGACTCAAG CTTTTTGTCT TGCACTGGTG CCACGCCAAG 
CTCGTTCAAG CATTTAGCCG GTGGAAAAAG GTGCCAACAG CATCATACAC CATCCATCTG 
CGACACCATA CAGAGGTGGT GGTGGTGGCT GCTCCGCCGC AAGCCTCCAA TATGGGGGAT 
CACAGTTACT GTAATTCCCT GAACAAACTC AATCCACACA AGCCCGTGCC TAGACTAAAA 
AACTCGACAC TTTCAACAGT GGATTGTGGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

H257_13996 ( XM_009841710.1 ) cDNA ORF clone, Aphanomyces astaci -> XP_009840012.1 Aphanomyces astaci hypothetical protein, variant 1 mRNA.

Price & Availability↑

CloneID	OAp113535
Clone ID Related Accession (Same CDS sequence)	XM_009841710.1
Accession Version	XM_009841710.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-20
Organism	Aphanomyces astaci
Product	hypothetical protein, variant 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_009798315).

ATGATCGAGG CGCTGCTGCT TTGCGCAGAC GCCACTGGTC GTACAGCTTT GCACTGGAGT 
GTCAGGAACA AACATGCGGC TGTGGCGTCG TACTTGCTCC AGTCAGCGGC GCTCGTGGGG 
GTGGTCGTTG AGCTGTTGCA AATGTGCGAC GATGTACATC ATTCGCCGCT TCACGTTGCG 
TGTGCCACTG GTCAAACGGC CCTGGCAGAG GCGATGCTGC AATTGGGGGC CAACGCCAAC 
ATGGCTACCA GAATACATGA GCCGCGCCCA CCGAGATGTC GACGCCGCTC CCAATCTAAA 
ATGTCTACTA CCTTGAATAC CTCTCAGCCC AACCGCCTAC GAGAAGCTAT GGAAACAATG 
TCGCTACATG ACGTGGCGCA ACACACAGTC GAGCTCAAGA CCTTGAAGCT GCTGGTACCT 
CGACCCCTAT CATCCCCGCA GCTACAATCA TCTACTGCAA CATCCTGCGA CACGGTGGAA 
CCGATAGCGT CCCCTCTAGC CATTGCATTG GTCGCTCGAT CATACGACAT TGCCGACCTG 
TTGTTGCAAT ATGGCGCCAA CATCATCGTG CGAGACCAAG TATGGAGAGC TTATGTGGAC 
GATGCCAACA GCCGTGCGAC GCTAGTCCAG TGGAGTTCAC GCTGGCTGTG TCCAGATATC 
TTTCCTTCAA GTGCTTTCGC CACACTGTGT GGCCAGGCTT CGTTTACTCC CAGTATCTTT 
CCCCGTAATG ACACCAACGA CAACTCAGAC GCAAAACATG CGATGACGGT TATCGAATCG 
GGACTTTGTG CTTTGTTGGA TGTGTCAATT GGGCTGAATT ATTACTCCAC TTGGGACAAT 
CTAGCCCACG CTGCATTCTC GGCGTTTCGC CTACGAAGAC TGCACTTGGC CCATCGCCTA 
GCCAACCTAT GCGGCGCTAC GTTGTTTAAC GATTCGCGCT CGACAAGTTG GCTAGTGGTA 
GCTGTGGAGA ATGCATGTCT CGCCAGCTGC CGGTGGCTTC AAGCTCATGA CTACCCCTTC 
TCAGCTATCG ACATCCCGGC CATGTGTCTA CTGCTGAGCT CTCCCTGTCG ACACCGATCC 
GTTAACGACG ACATTGCCGT TCAAACTCGG ACGAGTCTGT TTGTATGGCT CGTACAGTGC 
CATGTCAGCC TGATTCAGTC CTCCTACCTT CAAACGTATT TGGACCTGGC GTTGGCCAAT 
GCAAACACCG CTGTGACCCG ACTATTGGTG GATAACATGA TTCACACCGA GGACAAGAGC 
TACTACACCA TTCGTGCGGA CCACGCTGTG GTAGCCAGTC GTGGAGATGA AACATATATC 
CTGTCGCTTC GTTCGATGGG ATGCTGGACA CATTGCGACC TTGGGCTGAC GCTGGCCATC 
CATTTCAACA TGTCGCAGAC GATCCTGCGC TGTATTATTG CGCACGGCGC CCGACCTACC 
CCTCAAGCCG TCGTCTACGG CAAACCCGCC ATCGTGTGGG CCATCTGGCA TCGGCGCCTT 
GACATTGTTC GGGTCCTTTG TATCTTGGAG CCCACACTCT GTCTTGCGAC GACGGATGGT 
CAAGGACGAG ATGCATTGTA TTATGCCATG CGAACCAACG ATGTGTCCGT GGTGGCGTTT 
CTATGGACGA ACCGACGACA GAGCCAGTTC AACTTTGCCG CCGCATTGGT GGCTGCAGTG 
GAATCCAATG CTATGGCGTC CCTGAAATGG ATGGCTGCCA CGGCACCACA AGATTTTTTT 
CGGTCTATCG AGGCGCTACA CGTGCACATG CCACATGTGA CATCGTTGGA GCATGTAGCT 
TGTGCGCGAG GGTTTCTCGA GCTTGCGACG TGGCTACGCT CGATAAACAG CAACCCCACG 
ACGACATGCT TGTCATCGTC AAATGCTGAT GGGTGCACAC CTACGCAGGT CGCAAGGTTG 
TTTGGGTATC CAATACATTT CGACGAATCC AAGGGGGATA ACTGCTGCCA TGACCCTGTG 
GTGTTGAATG GTGTGCTGCG ACGGCTCTTG TCCACACAAG TGTCGGTCAT CTCTACGGGG 
AACAAGTCTA TCGATGGCAC TGCTGCTTGG CGGCCACTAT ATCCAATTGC AAGCCAAGCC 
TTTCAATTCA CCGAGCTATA CGCAGCGTGT AGCAACAACT CTGTCGCATA CGTCAGCATC 
TTGGCCTCGT TCGGAGTGTC CCTCGTCGAG CCCTGTGGAC CACTCAACCT GCCACCGTTG 
GATTGGGCAG CTCACTATGG AGCCGTCGAC GTGATCCAGT GCCTGCTTAG CCAAGGCGTG 
GTGGACGTAG AAGGGACGGC GCTGAGGCGG GCGGCTACCC GACGCCAGGG GTCAGCAGTT 
GCGGCTTTGC TGGATGCAGC TCCGCCTGCC ACGTCATTGG GGCGACTGCA AGGGGATGGG 
GCTACACCCA CACTGCTGCA TTTAGTAGCT CGTGTCCAAT CAACTGTTGC CCCGCGCTTG 
ATCCAAGTTT TGATAGACCA GTACCACGCG GATGTGGATA TTGTGGATGG CCAAGGGTTG 
ACACCCGTTG TGTATGCGAT GGTGAGTGGC AACGTGCCGG CGATGCTTTG TTTGTTGGAG 
CGAGGTGCAC GGCTGGAGGC CGAATACGAA GGCCAATCCG GATTCTACTA TGTCCTGCAC 
CTACTCCCCT CGGACACGTG GCGTGTCTTC TTTCAAGCGT ATTTGGGTGT GGTACTACAC 
GGCCGAGCAC TGCACTGCAC AGAATCGTGC GGATGCAAGT CGTTTGAAGG GTCGGTAGGT 
GACGTGGCGT GTTCGTTTTG CAGCCACCGA TTTGACCAAC ATTCCCACAT CCCCCTCCCC 
CCCTGGCATC AAGACGTGTC CGATACGTAC CGCATGCAAC GTACTAGTAG TACTACTAGT 
AGTCATATAT CCCCCGAATC AAGCCCCCGA TCCACAACAT CCGACAACGA CGACCACGAT 
AATGTCCATC GTTTCCAACA CGATCGTTCC GATTTCGAGA ATCAAGACCC CCATCTATCT 
CCGCTGCCAT TGTCCACACT AGAGTATGAC TACGTCGGTC GGTTGGATCA ATGTCATATT 
TCTGCGGTCG CCTGGGCACG ATTCCGCCCC GAAGTCTCGA TGTATTGTCT GTCGTCGATG 
ACTGGTCATC CGGATGCAGG TCCATCAAGC ACCACCGACG ACGACGACGA AGACAAAGAG 
GATGTGTTGC AACGCGGGTT TGTGTGTTCA TTGGACGATC AACCAGAGGC GTGGTCATGG 
AGGGTTCCGG CATTGAATCA ATCTGACATC CCGGTGAAGA TGGACAAACA ACTAATTCCC 
CCGAGGTGGT CTGTGGCTAC TACGTTCGGA CTAGAGTATC ACGTCGACTG CTGGTGTACA 
CAAGGGGGCA TGTACTGTCT GTCGGCCGCC ATGAGTTTAC GACGAGTGGT GACTTGGTGG 
CTAGGGAGAC GCCTTCGATC TACGGGATCT GCGACGACGA CAACGAAGAC CGTCACAACG 
TTGTTTCACA TATGGAAATC CGACGGTCGT CGTCGTATTT CGTCTCCGCG CGACCACGTG 
GTGCCCCGTA CGCGCCAGCA ACTAGTGCGA CTCAAGCTTT TTGTCTTGCA CTGGTGCCAC 
GCCAAGCTCG TTCAAGCATT TAGCCGGTGG AAAAAGGTGC CAACAGCATC ATACACCATC 
CATCTGCGAC ACCATACAGA GGTGGTGGTG GTGGCTGCTC CGCCGCAAGC CTCCAATATG 
GGGGATCACA GTTACTGTAA TTCCCTGAAC AAACTCAATC CACACAAGCC CGTGCCTAGA 
CTAAAAAACT CGACACTTTC AACAGTGGAT TGTGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009840012.1
CDS	219..4037
Translation	MIEALLLCADATGRTALHWSVRNKHAAVASYLLQSAALVGVVVELLQMCDDVHHSPLHVACATGQTALAEAMLQLGANANMATRIHEPRPPRCRRRSQSKMSTTLNTSQPNRLREAMETMSLHDVAQHTVELKTLKLLVPRPLSSPQLQSSTATSCDTVEPIASPLAIALVARSYDIADLLLQYGANIIVRDQVWRAYVDDANSRATLVQWSSRWLCPDIFPSSAFATLCGQASFTPSIFPRNDTNDNSDAKHAMTVIESGLCALLDVSIGLNYYSTWDNLAHAAFSAFRLRRLHLAHRLANLCGATLFNDSRSTSWLVVAVENACLASCRWLQAHDYPFSAIDIPAMCLLLSSPCRHRSVNDDIAVQTRTSLFVWLVQCHVSLIQSSYLQTYLDLALANANTAVTRLLVDNMIHTEDKSYYTIRADHAVVASRGDETYILSLRSMGCWTHCDLGLTLAIHFNMSQTILRCIIAHGARPTPQAVVYGKPAIVWAIWHRRLDIVRVLCILEPTLCLATTDGQGRDALYYAMRTNDVSVVAFLWTNRRQSQFNFAAALVAAVESNAMASLKWMAATAPQDFFRSIEALHVHMPHVTSLEHVACARGFLELATWLRSINSNPTTTCLSSSNADGCTPTQVARLFGYPIHFDESKGDNCCHDPVVLNGVLRRLLSTQVSVISTGNKSIDGTAAWRPLYPIASQAFQFTELYAACSNNSVAYVSILASFGVSLVEPCGPLNLPPLDWAAHYGAVDVIQCLLSQGVVDVEGTALRRAATRRQGSAVAALLDAAPPATSLGRLQGDGATPTLLHLVARVQSTVAPRLIQVLIDQYHADVDIVDGQGLTPVVYAMVSGNVPAMLCLLERGARLEAEYEGQSGFYYVLHLLPSDTWRVFFQAYLGVVLHGRALHCTESCGCKSFEGSVGDVACSFCSHRFDQHSHIPLPPWHQDVSDTYRMQRTSSTTSSHISPESSPRSTTSDNDDHDNVHRFQHDRSDFENQDPHLSPLPLSTLEYDYVGRLDQCHISAVAWARFRPEVSMYCLSSMTGHPDAGPSSTTDDDDEDKEDVLQRGFVCSLDDQPEAWSWRVPALNQSDIPVKMDKQLIPPRWSVATTFGLEYHVDCWCTQGGMYCLSAAMSLRRVVTWWLGRRLRSTGSATTTTKTVTTLFHIWKSDGRRRISSPRDHVVPRTRQQLVRLKLFVLHWCHAKLVQAFSRWKKVPTASYTIHLRHHTEVVVVAAPPQASNMGDHSYCNSLNKLNPHKPVPRLKNSTLSTVDCG

Target ORF information:

RefSeq Version	XM_009841710.1
Organism	Aphanomyces astaci
Definition	Aphanomyces astaci hypothetical protein, variant 1 mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009841710.1

ORF Insert Sequence:

ATGATCGAGG CGCTGCTGCT TTGCGCAGAC GCCACTGGTC GTACAGCTTT GCACTGGAGT 
GTCAGGAACA AACATGCGGC TGTGGCGTCG TACTTGCTCC AGTCAGCGGC GCTCGTGGGG 
GTGGTCGTTG AGCTGTTGCA AATGTGCGAC GATGTACATC ATTCGCCGCT TCACGTTGCG 
TGTGCCACTG GTCAAACGGC CCTGGCAGAG GCGATGCTGC AATTGGGGGC CAACGCCAAC 
ATGGCTACCA GAATACATGA GCCGCGCCCA CCGAGATGTC GACGCCGCTC CCAATCTAAA 
ATGTCTACTA CCTTGAATAC CTCTCAGCCC AACCGCCTAC GAGAAGCTAT GGAAACAATG 
TCGCTACATG ACGTGGCGCA ACACACAGTC GAGCTCAAGA CCTTGAAGCT GCTGGTACCT 
CGACCCCTAT CATCCCCGCA GCTACAATCA TCTACTGCAA CATCCTGCGA CACGGTGGAA 
CCGATAGCGT CCCCTCTAGC CATTGCATTG GTCGCTCGAT CATACGACAT TGCCGACCTG 
TTGTTGCAAT ATGGCGCCAA CATCATCGTG CGAGACCAAG TATGGAGAGC TTATGTGGAC 
GATGCCAACA GCCGTGCGAC GCTAGTCCAG TGGAGTTCAC GCTGGCTGTG TCCAGATATC 
TTTCCTTCAA GTGCTTTCGC CACACTGTGT GGCCAGGCTT CGTTTACTCC CAGTATCTTT 
CCCCGTAATG ACACCAACGA CAACTCAGAC GCAAAACATG CGATGACGGT TATCGAATCG 
GGACTTTGTG CTTTGTTGGA TGTGTCAATT GGGCTGAATT ATTACTCCAC TTGGGACAAT 
CTAGCCCACG CTGCATTCTC GGCGTTTCGC CTACGAAGAC TGCACTTGGC CCATCGCCTA 
GCCAACCTAT GCGGCGCTAC GTTGTTTAAC GATTCGCGCT CGACAAGTTG GCTAGTGGTA 
GCTGTGGAGA ATGCATGTCT CGCCAGCTGC CGGTGGCTTC AAGCTCATGA CTACCCCTTC 
TCAGCTATCG ACATCCCGGC CATGTGTCTA CTGCTGAGCT CTCCCTGTCG ACACCGATCC 
GTTAACGACG ACATTGCCGT TCAAACTCGG ACGAGTCTGT TTGTATGGCT CGTACAGTGC 
CATGTCAGCC TGATTCAGTC CTCCTACCTT CAAACGTATT TGGACCTGGC GTTGGCCAAT 
GCAAACACCG CTGTGACCCG ACTATTGGTG GATAACATGA TTCACACCGA GGACAAGAGC 
TACTACACCA TTCGTGCGGA CCACGCTGTG GTAGCCAGTC GTGGAGATGA AACATATATC 
CTGTCGCTTC GTTCGATGGG ATGCTGGACA CATTGCGACC TTGGGCTGAC GCTGGCCATC 
CATTTCAACA TGTCGCAGAC GATCCTGCGC TGTATTATTG CGCACGGCGC CCGACCTACC 
CCTCAAGCCG TCGTCTACGG CAAACCCGCC ATCGTGTGGG CCATCTGGCA TCGGCGCCTT 
GACATTGTTC GGGTCCTTTG TATCTTGGAG CCCACACTCT GTCTTGCGAC GACGGATGGT 
CAAGGACGAG ATGCATTGTA TTATGCCATG CGAACCAACG ATGTGTCCGT GGTGGCGTTT 
CTATGGACGA ACCGACGACA GAGCCAGTTC AACTTTGCCG CCGCATTGGT GGCTGCAGTG 
GAATCCAATG CTATGGCGTC CCTGAAATGG ATGGCTGCCA CGGCACCACA AGATTTTTTT 
CGGTCTATCG AGGCGCTACA CGTGCACATG CCACATGTGA CATCGTTGGA GCATGTAGCT 
TGTGCGCGAG GGTTTCTCGA GCTTGCGACG TGGCTACGCT CGATAAACAG CAACCCCACG 
ACGACATGCT TGTCATCGTC AAATGCTGAT GGGTGCACAC CTACGCAGGT CGCAAGGTTG 
TTTGGGTATC CAATACATTT CGACGAATCC AAGGGGGATA ACTGCTGCCA TGACCCTGTG 
GTGTTGAATG GTGTGCTGCG ACGGCTCTTG TCCACACAAG TGTCGGTCAT CTCTACGGGG 
AACAAGTCTA TCGATGGCAC TGCTGCTTGG CGGCCACTAT ATCCAATTGC AAGCCAAGCC 
TTTCAATTCA CCGAGCTATA CGCAGCGTGT AGCAACAACT CTGTCGCATA CGTCAGCATC 
TTGGCCTCGT TCGGAGTGTC CCTCGTCGAG CCCTGTGGAC CACTCAACCT GCCACCGTTG 
GATTGGGCAG CTCACTATGG AGCCGTCGAC GTGATCCAGT GCCTGCTTAG CCAAGGCGTG 
GTGGACGTAG AAGGGACGGC GCTGAGGCGG GCGGCTACCC GACGCCAGGG GTCAGCAGTT 
GCGGCTTTGC TGGATGCAGC TCCGCCTGCC ACGTCATTGG GGCGACTGCA AGGGGATGGG 
GCTACACCCA CACTGCTGCA TTTAGTAGCT CGTGTCCAAT CAACTGTTGC CCCGCGCTTG 
ATCCAAGTTT TGATAGACCA GTACCACGCG GATGTGGATA TTGTGGATGG CCAAGGGTTG 
ACACCCGTTG TGTATGCGAT GGTGAGTGGC AACGTGCCGG CGATGCTTTG TTTGTTGGAG 
CGAGGTGCAC GGCTGGAGGC CGAATACGAA GGCCAATCCG GATTCTACTA TGTCCTGCAC 
CTACTCCCCT CGGACACGTG GCGTGTCTTC TTTCAAGCGT ATTTGGGTGT GGTACTACAC 
GGCCGAGCAC TGCACTGCAC AGAATCGTGC GGATGCAAGT CGTTTGAAGG GTCGGTAGGT 
GACGTGGCGT GTTCGTTTTG CAGCCACCGA TTTGACCAAC ATTCCCACAT CCCCCTCCCC 
CCCTGGCATC AAGACGTGTC CGATACGTAC CGCATGCAAC GTACTAGTAG TACTACTAGT 
AGTCATATAT CCCCCGAATC AAGCCCCCGA TCCACAACAT CCGACAACGA CGACCACGAT 
AATGTCCATC GTTTCCAACA CGATCGTTCC GATTTCGAGA ATCAAGACCC CCATCTATCT 
CCGCTGCCAT TGTCCACACT AGAGTATGAC TACGTCGGTC GGTTGGATCA ATGTCATATT 
TCTGCGGTCG CCTGGGCACG ATTCCGCCCC GAAGTCTCGA TGTATTGTCT GTCGTCGATG 
ACTGGTCATC CGGATGCAGG TCCATCAAGC ACCACCGACG ACGACGACGA AGACAAAGAG 
GATGTGTTGC AACGCGGGTT TGTGTGTTCA TTGGACGATC AACCAGAGGC GTGGTCATGG 
AGGGTTCCGG CATTGAATCA ATCTGACATC CCGGTGAAGA TGGACAAACA ACTAATTCCC 
CCGAGGTGGT CTGTGGCTAC TACGTTCGGA CTAGAGTATC ACGTCGACTG CTGGTGTACA 
CAAGGGGGCA TGTACTGTCT GTCGGCCGCC ATGAGTTTAC GACGAGTGGT GACTTGGTGG 
CTAGGGAGAC GCCTTCGATC TACGGGATCT GCGACGACGA CAACGAAGAC CGTCACAACG 
TTGTTTCACA TATGGAAATC CGACGGTCGT CGTCGTATTT CGTCTCCGCG CGACCACGTG 
GTGCCCCGTA CGCGCCAGCA ACTAGTGCGA CTCAAGCTTT TTGTCTTGCA CTGGTGCCAC 
GCCAAGCTCG TTCAAGCATT TAGCCGGTGG AAAAAGGTGC CAACAGCATC ATACACCATC 
CATCTGCGAC ACCATACAGA GGTGGTGGTG GTGGCTGCTC CGCCGCAAGC CTCCAATATG 
GGGGATCACA GTTACTGTAA TTCCCTGAAC AAACTCAATC CACACAAGCC CGTGCCTAGA 
CTAAAAAACT CGACACTTTC AACAGTGGAT TGTGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

H257_13996 ( XM_009841709.1 ) cDNA ORF clone, Aphanomyces astaci -> XP_009840011.1 Aphanomyces astaci hypothetical protein mRNA.

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CloneID	OAp113534
Clone ID Related Accession (Same CDS sequence)	XM_009841709.1
Accession Version	XM_009841709.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4668bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-20
Organism	Aphanomyces astaci
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_009798315).

ATGGGCGAGT ATGTCAAGCT CATACGACAG CACCGCCCAC CAAATCGATT TCGCCCCCGA 
ACCCGCCACG AATCTTGGTT TCTTCGGTAC ACGGCAACCC GCGTAGGCGG CGACGATGCC 
GACAACGTAG AGTTGCACCC GTGGCACTCG AAATTACACC TATGGCAACT GGCGAGTCTC 
CAGTTTACGT CATTGCACGA AGCGTGTCGT GTGGGGGATG TGAAGGCGTT GAATATGGCG 
CTACAAGAAA CATGCACCAT GCACCAAGAC TGCACCGGGA GTACGCCACT GCATGTAGCC 
GCGACCCATG GCCACCTCTC CTGTGTGCTT CGCCTGTTGG AACTTCCAGA CGCAATGCTT 
CAGTTGCACG TCGTAGATGT AGCCGGACGC ACCGCGCTAC ACAAGGCATT GCAGCGTCCA 
TGCCCAAGTG TGTTGGCTTG CGTAGATGCC ATGCTAGCCC ATGCACCGCC TTCACTGCTC 
ACGAATGTGC ACGACAAGTA TGGTTGGTCG ATTCAAGCCC TAGACGTTCG CCAGCGGGGA 
GATTTGTTTG ATGCAGTGTC AACCGGAAAT GTTCACCGCA TGCAATTTAT CCTCTCAAAT 
TACGATCTAA GATCATCCAA CTGGACAGTA TTGCCGTTGT TGGAATGGAC ATTGCTCCAC 
CAAGCGTGCT TAAGCCAGCA AGTTGCCGCC ACAAAGGAAG TGTTGGTCAA CCGCCGCCTG 
TTGTGCAGCG TCGACCCACT AAAGCAAGAT TCATCTGGGT GTACCGCCTT GCACGTGAGT 
GCCAAGGGCG GCTTTATTGA AGGCTGCGTC CTGCTCTTGG ACGCCAACTC GTCAAATCAA 
CCAGTCGACA TGATCGAGGC GCTGCTGCTT TGCGCAGACG CCACTGGTCG TACAGCTTTG 
CACTGGAGTG TCAGGAACAA ACATGCGGCT GTGGCGTCGT ACTTGCTCCA GTCAGCGGCG 
CTCGTGGGGG TGGTCGTTGA GCTGTTGCAA ATGTGCGACG ATGTACATCA TTCGCCGCTT 
CACGTTGCGT GTGCCACTGG TCAAACGGCC CTGGCAGAGG CGATGCTGCA ATTGGGGGCC 
AACGCCAACA TGGCTACCAG AATACATGAG CCGCGCCCAC CGAGATGTCG ACGCCGCTCC 
CAATCTAAAA TGTCTACTAC CTTGAATACC TCTCAGCCCA ACCGCCTACG AGAAGCTATG 
GAAACAATGT CGCTACATGA CGTGGCGCAA CACACAGTCG AGCTCAAGAC CTTGAAGCTG 
CTGGTACCTC GACCCCTATC ATCCCCGCAG CTACAATCAT CTACTGCAAC ATCCTGCGAC 
ACGGTGGAAC CGATAGCGTC CCCTCTAGCC ATTGCATTGG TCGCTCGATC ATACGACATT 
GCCGACCTGT TGTTGCAATA TGGCGCCAAC ATCATCGTGC GAGACCAAGT ATGGAGAGCT 
TATGTGGACG ATGCCAACAG CCGTGCGACG CTAGTCCAGT GGAGTTCACG CTGGCTGTGT 
CCAGATATCT TTCCTTCAAG TGCTTTCGCC ACACTGTGTG GCCAGGCTTC GTTTACTCCC 
AGTATCTTTC CCCGTAATGA CACCAACGAC AACTCAGACG CAAAACATGC GATGACGGTT 
ATCGAATCGG GACTTTGTGC TTTGTTGGAT GTGTCAATTG GGCTGAATTA TTACTCCACT 
TGGGACAATC TAGCCCACGC TGCATTCTCG GCGTTTCGCC TACGAAGACT GCACTTGGCC 
CATCGCCTAG CCAACCTATG CGGCGCTACG TTGTTTAACG ATTCGCGCTC GACAAGTTGG 
CTAGTGGTAG CTGTGGAGAA TGCATGTCTC GCCAGCTGCC GGTGGCTTCA AGCTCATGAC 
TACCCCTTCT CAGCTATCGA CATCCCGGCC ATGTGTCTAC TGCTGAGCTC TCCCTGTCGA 
CACCGATCCG TTAACGACGA CATTGCCGTT CAAACTCGGA CGAGTCTGTT TGTATGGCTC 
GTACAGTGCC ATGTCAGCCT GATTCAGTCC TCCTACCTTC AAACGTATTT GGACCTGGCG 
TTGGCCAATG CAAACACCGC TGTGACCCGA CTATTGGTGG ATAACATGAT TCACACCGAG 
GACAAGAGCT ACTACACCAT TCGTGCGGAC CACGCTGTGG TAGCCAGTCG TGGAGATGAA 
ACATATATCC TGTCGCTTCG TTCGATGGGA TGCTGGACAC ATTGCGACCT TGGGCTGACG 
CTGGCCATCC ATTTCAACAT GTCGCAGACG ATCCTGCGCT GTATTATTGC GCACGGCGCC 
CGACCTACCC CTCAAGCCGT CGTCTACGGC AAACCCGCCA TCGTGTGGGC CATCTGGCAT 
CGGCGCCTTG ACATTGTTCG GGTCCTTTGT ATCTTGGAGC CCACACTCTG TCTTGCGACG 
ACGGATGGTC AAGGACGAGA TGCATTGTAT TATGCCATGC GAACCAACGA TGTGTCCGTG 
GTGGCGTTTC TATGGACGAA CCGACGACAG AGCCAGTTCA ACTTTGCCGC CGCATTGGTG 
GCTGCAGTGG AATCCAATGC TATGGCGTCC CTGAAATGGA TGGCTGCCAC GGCACCACAA 
GATTTTTTTC GGTCTATCGA GGCGCTACAC GTGCACATGC CACATGTGAC ATCGTTGGAG 
CATGTAGCTT GTGCGCGAGG GTTTCTCGAG CTTGCGACGT GGCTACGCTC GATAAACAGC 
AACCCCACGA CGACATGCTT GTCATCGTCA AATGCTGATG GGTGCACACC TACGCAGGTC 
GCAAGGTTGT TTGGGTATCC AATACATTTC GACGAATCCA AGGGGGATAA CTGCTGCCAT 
GACCCTGTGG TGTTGAATGG TGTGCTGCGA CGGCTCTTGT CCACACAAGT GTCGGTCATC 
TCTACGGGGA ACAAGTCTAT CGATGGCACT GCTGCTTGGC GGCCACTATA TCCAATTGCA 
AGCCAAGCCT TTCAATTCAC CGAGCTATAC GCAGCGTGTA GCAACAACTC TGTCGCATAC 
GTCAGCATCT TGGCCTCGTT CGGAGTGTCC CTCGTCGAGC CCTGTGGACC ACTCAACCTG 
CCACCGTTGG ATTGGGCAGC TCACTATGGA GCCGTCGACG TGATCCAGTG CCTGCTTAGC 
CAAGGCGTGG TGGACGTAGA AGGGACGGCG CTGAGGCGGG CGGCTACCCG ACGCCAGGGG 
TCAGCAGTTG CGGCTTTGCT GGATGCAGCT CCGCCTGCCA CGTCATTGGG GCGACTGCAA 
GGGGATGGGG CTACACCCAC ACTGCTGCAT TTAGTAGCTC GTGTCCAATC AACTGTTGCC 
CCGCGCTTGA TCCAAGTTTT GATAGACCAG TACCACGCGG ATGTGGATAT TGTGGATGGC 
CAAGGGTTGA CACCCGTTGT GTATGCGATG GTGAGTGGCA ACGTGCCGGC GATGCTTTGT 
TTGTTGGAGC GAGGTGCACG GCTGGAGGCC GAATACGAAG GCCAATCCGG ATTCTACTAT 
GTCCTGCACC TACTCCCCTC GGACACGTGG CGTGTCTTCT TTCAAGCGTA TTTGGGTGTG 
GTACTACACG GCCGAGCACT GCACTGCACA GAATCGTGCG GATGCAAGTC GTTTGAAGGG 
TCGGTAGGTG ACGTGGCGTG TTCGTTTTGC AGCCACCGAT TTGACCAACA TTCCCACATC 
CCCCTCCCCC CCTGGCATCA AGACGTGTCC GATACGTACC GCATGCAACG TACTAGTAGT 
ACTACTAGTA GTCATATATC CCCCGAATCA AGCCCCCGAT CCACAACATC CGACAACGAC 
GACCACGATA ATGTCCATCG TTTCCAACAC GATCGTTCCG ATTTCGAGAA TCAAGACCCC 
CATCTATCTC CGCTGCCATT GTCCACACTA GAGTATGACT ACGTCGGTCG GTTGGATCAA 
TGTCATATTT CTGCGGTCGC CTGGGCACGA TTCCGCCCCG AAGTCTCGAT GTATTGTCTG 
TCGTCGATGA CTGGTCATCC GGATGCAGGT CCATCAAGCA CCACCGACGA CGACGACGAA 
GACAAAGAGG ATGTGTTGCA ACGCGGGTTT GTGTGTTCAT TGGACGATCA ACCAGAGGCG 
TGGTCATGGA GGGTTCCGGC ATTGAATCAA TCTGACATCC CGGTGAAGAT GGACAAACAA 
CTAATTCCCC CGAGGTGGTC TGTGGCTACT ACGTTCGGAC TAGAGTATCA CGTCGACTGC 
TGGTGTACAC AAGGGGGCAT GTACTGTCTG TCGGCCGCCA TGAGTTTACG ACGAGTGGTG 
ACTTGGTGGC TAGGGAGACG CCTTCGATCT ACGGGATCTG CGACGACGAC AACGAAGACC 
GTCACAACGT TGTTTCACAT ATGGAAATCC GACGGTCGTC GTCGTATTTC GTCTCCGCGC 
GACCACGTGG TGCCCCGTAC GCGCCAGCAA CTAGTGCGAC TCAAGCTTTT TGTCTTGCAC 
TGGTGCCACG CCAAGCTCGT TCAAGCATTT AGCCGGTGGA AAAAGGTGCC AACAGCATCA 
TACACCATCC ATCTGCGACA CCATACAGAG GTGGTGGTGG TGGCTGCTCC GCCGCAAGCC 
TCCAATATGG GGGATCACAG TTACTGTAAT TCCCTGAACA AACTCAATCC ACACAAGCCC 
GTGCCTAGAC TAAAAAACTC GACACTTTCA ACAGTGGATT GTGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009840011.1
CDS	19..4686
Translation	MGEYVKLIRQHRPPNRFRPRTRHESWFLRYTATRVGGDDADNVELHPWHSKLHLWQLASLQFTSLHEACRVGDVKALNMALQETCTMHQDCTGSTPLHVAATHGHLSCVLRLLELPDAMLQLHVVDVAGRTALHKALQRPCPSVLACVDAMLAHAPPSLLTNVHDKYGWSIQALDVRQRGDLFDAVSTGNVHRMQFILSNYDLRSSNWTVLPLLEWTLLHQACLSQQVAATKEVLVNRRLLCSVDPLKQDSSGCTALHVSAKGGFIEGCVLLLDANSSNQPVDMIEALLLCADATGRTALHWSVRNKHAAVASYLLQSAALVGVVVELLQMCDDVHHSPLHVACATGQTALAEAMLQLGANANMATRIHEPRPPRCRRRSQSKMSTTLNTSQPNRLREAMETMSLHDVAQHTVELKTLKLLVPRPLSSPQLQSSTATSCDTVEPIASPLAIALVARSYDIADLLLQYGANIIVRDQVWRAYVDDANSRATLVQWSSRWLCPDIFPSSAFATLCGQASFTPSIFPRNDTNDNSDAKHAMTVIESGLCALLDVSIGLNYYSTWDNLAHAAFSAFRLRRLHLAHRLANLCGATLFNDSRSTSWLVVAVENACLASCRWLQAHDYPFSAIDIPAMCLLLSSPCRHRSVNDDIAVQTRTSLFVWLVQCHVSLIQSSYLQTYLDLALANANTAVTRLLVDNMIHTEDKSYYTIRADHAVVASRGDETYILSLRSMGCWTHCDLGLTLAIHFNMSQTILRCIIAHGARPTPQAVVYGKPAIVWAIWHRRLDIVRVLCILEPTLCLATTDGQGRDALYYAMRTNDVSVVAFLWTNRRQSQFNFAAALVAAVESNAMASLKWMAATAPQDFFRSIEALHVHMPHVTSLEHVACARGFLELATWLRSINSNPTTTCLSSSNADGCTPTQVARLFGYPIHFDESKGDNCCHDPVVLNGVLRRLLSTQVSVISTGNKSIDGTAAWRPLYPIASQAFQFTELYAACSNNSVAYVSILASFGVSLVEPCGPLNLPPLDWAAHYGAVDVIQCLLSQGVVDVEGTALRRAATRRQGSAVAALLDAAPPATSLGRLQGDGATPTLLHLVARVQSTVAPRLIQVLIDQYHADVDIVDGQGLTPVVYAMVSGNVPAMLCLLERGARLEAEYEGQSGFYYVLHLLPSDTWRVFFQAYLGVVLHGRALHCTESCGCKSFEGSVGDVACSFCSHRFDQHSHIPLPPWHQDVSDTYRMQRTSSTTSSHISPESSPRSTTSDNDDHDNVHRFQHDRSDFENQDPHLSPLPLSTLEYDYVGRLDQCHISAVAWARFRPEVSMYCLSSMTGHPDAGPSSTTDDDDEDKEDVLQRGFVCSLDDQPEAWSWRVPALNQSDIPVKMDKQLIPPRWSVATTFGLEYHVDCWCTQGGMYCLSAAMSLRRVVTWWLGRRLRSTGSATTTTKTVTTLFHIWKSDGRRRISSPRDHVVPRTRQQLVRLKLFVLHWCHAKLVQAFSRWKKVPTASYTIHLRHHTEVVVVAAPPQASNMGDHSYCNSLNKLNPHKPVPRLKNSTLSTVDCG

Target ORF information:

RefSeq Version	XM_009841709.1
Organism	Aphanomyces astaci
Definition	Aphanomyces astaci hypothetical protein mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009841709.1

ORF Insert Sequence:

ATGGGCGAGT ATGTCAAGCT CATACGACAG CACCGCCCAC CAAATCGATT TCGCCCCCGA 
ACCCGCCACG AATCTTGGTT TCTTCGGTAC ACGGCAACCC GCGTAGGCGG CGACGATGCC 
GACAACGTAG AGTTGCACCC GTGGCACTCG AAATTACACC TATGGCAACT GGCGAGTCTC 
CAGTTTACGT CATTGCACGA AGCGTGTCGT GTGGGGGATG TGAAGGCGTT GAATATGGCG 
CTACAAGAAA CATGCACCAT GCACCAAGAC TGCACCGGGA GTACGCCACT GCATGTAGCC 
GCGACCCATG GCCACCTCTC CTGTGTGCTT CGCCTGTTGG AACTTCCAGA CGCAATGCTT 
CAGTTGCACG TCGTAGATGT AGCCGGACGC ACCGCGCTAC ACAAGGCATT GCAGCGTCCA 
TGCCCAAGTG TGTTGGCTTG CGTAGATGCC ATGCTAGCCC ATGCACCGCC TTCACTGCTC 
ACGAATGTGC ACGACAAGTA TGGTTGGTCG ATTCAAGCCC TAGACGTTCG CCAGCGGGGA 
GATTTGTTTG ATGCAGTGTC AACCGGAAAT GTTCACCGCA TGCAATTTAT CCTCTCAAAT 
TACGATCTAA GATCATCCAA CTGGACAGTA TTGCCGTTGT TGGAATGGAC ATTGCTCCAC 
CAAGCGTGCT TAAGCCAGCA AGTTGCCGCC ACAAAGGAAG TGTTGGTCAA CCGCCGCCTG 
TTGTGCAGCG TCGACCCACT AAAGCAAGAT TCATCTGGGT GTACCGCCTT GCACGTGAGT 
GCCAAGGGCG GCTTTATTGA AGGCTGCGTC CTGCTCTTGG ACGCCAACTC GTCAAATCAA 
CCAGTCGACA TGATCGAGGC GCTGCTGCTT TGCGCAGACG CCACTGGTCG TACAGCTTTG 
CACTGGAGTG TCAGGAACAA ACATGCGGCT GTGGCGTCGT ACTTGCTCCA GTCAGCGGCG 
CTCGTGGGGG TGGTCGTTGA GCTGTTGCAA ATGTGCGACG ATGTACATCA TTCGCCGCTT 
CACGTTGCGT GTGCCACTGG TCAAACGGCC CTGGCAGAGG CGATGCTGCA ATTGGGGGCC 
AACGCCAACA TGGCTACCAG AATACATGAG CCGCGCCCAC CGAGATGTCG ACGCCGCTCC 
CAATCTAAAA TGTCTACTAC CTTGAATACC TCTCAGCCCA ACCGCCTACG AGAAGCTATG 
GAAACAATGT CGCTACATGA CGTGGCGCAA CACACAGTCG AGCTCAAGAC CTTGAAGCTG 
CTGGTACCTC GACCCCTATC ATCCCCGCAG CTACAATCAT CTACTGCAAC ATCCTGCGAC 
ACGGTGGAAC CGATAGCGTC CCCTCTAGCC ATTGCATTGG TCGCTCGATC ATACGACATT 
GCCGACCTGT TGTTGCAATA TGGCGCCAAC ATCATCGTGC GAGACCAAGT ATGGAGAGCT 
TATGTGGACG ATGCCAACAG CCGTGCGACG CTAGTCCAGT GGAGTTCACG CTGGCTGTGT 
CCAGATATCT TTCCTTCAAG TGCTTTCGCC ACACTGTGTG GCCAGGCTTC GTTTACTCCC 
AGTATCTTTC CCCGTAATGA CACCAACGAC AACTCAGACG CAAAACATGC GATGACGGTT 
ATCGAATCGG GACTTTGTGC TTTGTTGGAT GTGTCAATTG GGCTGAATTA TTACTCCACT 
TGGGACAATC TAGCCCACGC TGCATTCTCG GCGTTTCGCC TACGAAGACT GCACTTGGCC 
CATCGCCTAG CCAACCTATG CGGCGCTACG TTGTTTAACG ATTCGCGCTC GACAAGTTGG 
CTAGTGGTAG CTGTGGAGAA TGCATGTCTC GCCAGCTGCC GGTGGCTTCA AGCTCATGAC 
TACCCCTTCT CAGCTATCGA CATCCCGGCC ATGTGTCTAC TGCTGAGCTC TCCCTGTCGA 
CACCGATCCG TTAACGACGA CATTGCCGTT CAAACTCGGA CGAGTCTGTT TGTATGGCTC 
GTACAGTGCC ATGTCAGCCT GATTCAGTCC TCCTACCTTC AAACGTATTT GGACCTGGCG 
TTGGCCAATG CAAACACCGC TGTGACCCGA CTATTGGTGG ATAACATGAT TCACACCGAG 
GACAAGAGCT ACTACACCAT TCGTGCGGAC CACGCTGTGG TAGCCAGTCG TGGAGATGAA 
ACATATATCC TGTCGCTTCG TTCGATGGGA TGCTGGACAC ATTGCGACCT TGGGCTGACG 
CTGGCCATCC ATTTCAACAT GTCGCAGACG ATCCTGCGCT GTATTATTGC GCACGGCGCC 
CGACCTACCC CTCAAGCCGT CGTCTACGGC AAACCCGCCA TCGTGTGGGC CATCTGGCAT 
CGGCGCCTTG ACATTGTTCG GGTCCTTTGT ATCTTGGAGC CCACACTCTG TCTTGCGACG 
ACGGATGGTC AAGGACGAGA TGCATTGTAT TATGCCATGC GAACCAACGA TGTGTCCGTG 
GTGGCGTTTC TATGGACGAA CCGACGACAG AGCCAGTTCA ACTTTGCCGC CGCATTGGTG 
GCTGCAGTGG AATCCAATGC TATGGCGTCC CTGAAATGGA TGGCTGCCAC GGCACCACAA 
GATTTTTTTC GGTCTATCGA GGCGCTACAC GTGCACATGC CACATGTGAC ATCGTTGGAG 
CATGTAGCTT GTGCGCGAGG GTTTCTCGAG CTTGCGACGT GGCTACGCTC GATAAACAGC 
AACCCCACGA CGACATGCTT GTCATCGTCA AATGCTGATG GGTGCACACC TACGCAGGTC 
GCAAGGTTGT TTGGGTATCC AATACATTTC GACGAATCCA AGGGGGATAA CTGCTGCCAT 
GACCCTGTGG TGTTGAATGG TGTGCTGCGA CGGCTCTTGT CCACACAAGT GTCGGTCATC 
TCTACGGGGA ACAAGTCTAT CGATGGCACT GCTGCTTGGC GGCCACTATA TCCAATTGCA 
AGCCAAGCCT TTCAATTCAC CGAGCTATAC GCAGCGTGTA GCAACAACTC TGTCGCATAC 
GTCAGCATCT TGGCCTCGTT CGGAGTGTCC CTCGTCGAGC CCTGTGGACC ACTCAACCTG 
CCACCGTTGG ATTGGGCAGC TCACTATGGA GCCGTCGACG TGATCCAGTG CCTGCTTAGC 
CAAGGCGTGG TGGACGTAGA AGGGACGGCG CTGAGGCGGG CGGCTACCCG ACGCCAGGGG 
TCAGCAGTTG CGGCTTTGCT GGATGCAGCT CCGCCTGCCA CGTCATTGGG GCGACTGCAA 
GGGGATGGGG CTACACCCAC ACTGCTGCAT TTAGTAGCTC GTGTCCAATC AACTGTTGCC 
CCGCGCTTGA TCCAAGTTTT GATAGACCAG TACCACGCGG ATGTGGATAT TGTGGATGGC 
CAAGGGTTGA CACCCGTTGT GTATGCGATG GTGAGTGGCA ACGTGCCGGC GATGCTTTGT 
TTGTTGGAGC GAGGTGCACG GCTGGAGGCC GAATACGAAG GCCAATCCGG ATTCTACTAT 
GTCCTGCACC TACTCCCCTC GGACACGTGG CGTGTCTTCT TTCAAGCGTA TTTGGGTGTG 
GTACTACACG GCCGAGCACT GCACTGCACA GAATCGTGCG GATGCAAGTC GTTTGAAGGG 
TCGGTAGGTG ACGTGGCGTG TTCGTTTTGC AGCCACCGAT TTGACCAACA TTCCCACATC 
CCCCTCCCCC CCTGGCATCA AGACGTGTCC GATACGTACC GCATGCAACG TACTAGTAGT 
ACTACTAGTA GTCATATATC CCCCGAATCA AGCCCCCGAT CCACAACATC CGACAACGAC 
GACCACGATA ATGTCCATCG TTTCCAACAC GATCGTTCCG ATTTCGAGAA TCAAGACCCC 
CATCTATCTC CGCTGCCATT GTCCACACTA GAGTATGACT ACGTCGGTCG GTTGGATCAA 
TGTCATATTT CTGCGGTCGC CTGGGCACGA TTCCGCCCCG AAGTCTCGAT GTATTGTCTG 
TCGTCGATGA CTGGTCATCC GGATGCAGGT CCATCAAGCA CCACCGACGA CGACGACGAA 
GACAAAGAGG ATGTGTTGCA ACGCGGGTTT GTGTGTTCAT TGGACGATCA ACCAGAGGCG 
TGGTCATGGA GGGTTCCGGC ATTGAATCAA TCTGACATCC CGGTGAAGAT GGACAAACAA 
CTAATTCCCC CGAGGTGGTC TGTGGCTACT ACGTTCGGAC TAGAGTATCA CGTCGACTGC 
TGGTGTACAC AAGGGGGCAT GTACTGTCTG TCGGCCGCCA TGAGTTTACG ACGAGTGGTG 
ACTTGGTGGC TAGGGAGACG CCTTCGATCT ACGGGATCTG CGACGACGAC AACGAAGACC 
GTCACAACGT TGTTTCACAT ATGGAAATCC GACGGTCGTC GTCGTATTTC GTCTCCGCGC 
GACCACGTGG TGCCCCGTAC GCGCCAGCAA CTAGTGCGAC TCAAGCTTTT TGTCTTGCAC 
TGGTGCCACG CCAAGCTCGT TCAAGCATTT AGCCGGTGGA AAAAGGTGCC AACAGCATCA 
TACACCATCC ATCTGCGACA CCATACAGAG GTGGTGGTGG TGGCTGCTCC GCCGCAAGCC 
TCCAATATGG GGGATCACAG TTACTGTAAT TCCCTGAACA AACTCAATCC ACACAAGCCC 
GTGCCTAGAC TAAAAAACTC GACACTTTCA ACAGTGGATT GTGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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H257_13996 cDNA ORF clone, Aphanomyces astaci

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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