Mrjp1 cDNA ORF clone, Apis mellifera(honey bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mrjp1
Entrez Gene ID	406090
Full Name	major royal jelly protein 1
Synonyms	GB14888,p56kP-4
Gene Type	protein-coding
Organism	Apis mellifera(honey bee)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001011579.1	NP_001011579.1	major royal jelly protein 1 precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Characterizing the Structure and Oligomerization of Major Royal Jelly Protein 1 (MRJP1) by Mass Spectrometry and Complementary Biophysical Tools.
Mandacaru SC, do Vale LH, Vahidi S, Xiao Y, Skinner OS, Ricart CA, Kelleher NL, de Sousa MV, Konermann L
Biochemistry56(11)1645-1655(2017 03)

Finding the missing honey bee genes: lessons learned from a genome upgrade.
Elsik CG, Worley KC, Bennett AK, Beye M, Camara F, Childers CP, de Graaf DC, Debyser G, Deng J, Devreese B, Elhaik E, Evans JD, Foster LJ, Graur D, Guigo R, , Hoff KJ, Holder ME, Hudson ME, Hunt GJ, Jiang H, Joshi V, Khetani RS, Kosarev P, Kovar CL, Ma J, Maleszka R, Moritz RF, Munoz-Torres MC, Murphy TD, Muzny DM, Newsham IF, Reese JT, Robertson HM, Robinson GE, Rueppell O, Solovyev V, Stanke M, Stolle E, Tsuruda JM, Vaerenbergh MV, Waterhouse RM, Weaver DB, Whitfield CW, Wu Y, Zdobnov EM, Zhang L, Zhu D, Gibbs RA,
BMC genomics1586(2014 Jan)

Quantification of major royal jelly protein 1 in fresh royal jelly by indirect enzyme-linked immunosorbent assay.
Yamaguchi K, He S, Li Z, Murata K, Hitomi N, Mozumi M, Ariga R, Enomoto T
Bioscience, biotechnology, and biochemistry77(6)1310-2(2013)

Evolution of the Yellow/Major Royal Jelly Protein family and the emergence of social behavior in honey bees.
Drapeau MD, Albert S, Kucharski R, Prusko C, Maleszka R
Genome research16(11)1385-94(2006 Nov)

Insights into social insects from the genome of the honeybee Apis mellifera.

Nature443(7114)931-49(2006 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mass spectrometry data demonstrate that the native complexes of royal jelly predominantly exist in a four MRJP1 and four apisimin molecules stoichiometry.
Title: Characterizing the Structure and Oligomerization of Major Royal Jelly Protein 1 (MRJP1) by Mass Spectrometry and Complementary Biophysical Tools.

These results suggest that MRJP1 exists mainly as apisin in royal jelly.
Title: Quantification of major royal jelly protein 1 in fresh royal jelly by indirect enzyme-linked immunosorbent assay.

The following Mrjp1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mrjp1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHd00048	NM_001011579.1 Latest version!	Apis mellifera major royal jelly protein 1 (Mrjp1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mrjp1 ( NM_001011579.1 ) cDNA ORF clone, Apis mellifera(honey bee) -> NP_001011579.1 Apis mellifera major royal jelly protein 1 (Mrjp1), mRNA.

Price & Availability↑

CloneID	OHd00048
Clone ID Related Accession (Same CDS sequence)	NM_001011579.1
Accession Version	NM_001011579.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1299bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Apis mellifera(honey bee)
Product	major royal jelly protein 1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF000633.1. On Feb 5, 2005 this sequence version replaced XM_393380.1. ##Evidence-Data-START## Transcript exon combination :: AF000633.1, D79207.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00113341, SAMN00120364 [ECO:0000348] ##Evidence-Data-END##

ATGACAAGAT TGTTTATGCT GGTATGCCTT GGCATAGTTT GTCAAGGTAC GACAGGCAAC 
ATTCTTCGAG GAGAGTCTTT AAACAAATCA TTACCCATCC TTCACGAATG GAAATTCTTT 
GATTATGATT TCGGTAGCGA TGAAAGAAGA CAAGATGCAA TTCTATCTGG CGAATACGAC 
TACAAGAATA ATTATCCATC CGACATTGAC CAATGGCATG ATAAGATTTT TGTCACCATG 
CTGAGATACA ATGGCGTACC TTCCTCTTTG AACGTGATAT CTAAAAAGGT CGGTGATGGT 
GGTCCTCTTC TACAACCTTA TCCCGATTGG TCGTTTGCTA AATATGACGA TTGCTCTGGA 
ATCGTGAGCG CCTCAAAACT TGCGATCGAC AAATGCGACA GATTGTGGGT TCTGGACTCA 
GGTCTTGTCA ATAATACTCA ACCCATGTGT TCTCCAAAAC TGCTCACCTT TGATCTGACT 
ACCTCGCAAT TGCTCAAGCA AGTTGAAATA CCACATGATG TTGCCGTAAA TGCCACTACA 
GGAAAGGGAA GATTATCATC TCTAGCTGTT CAATCTTTAG ATTGCAATAC AAATAGCGAT 
ACTATGGTGT ACATAGCAGA CGAGAAAGGT GAAGGTTTAA TCGTGTATCA TAATTCTGAT 
GATTCCTTCC ATCGATTGAC TTCCAACACT TTCGATTACG ATCCTAAATT TACCAAAATG 
ACGATCGATG GAGAAAGTTA CACAGCCCAA GATGGAATTT CTGGAATGGC TCTTAGTCCC 
ATGACTAACA ATCTCTATTA CAGTCCTGTA GCTTCCACCA GTTTGTATTA TGTTAACACG 
GAACAATTCA GAACATCCGA TTATCAACAG AATGACATAC ATTACGAAGG AGTCCAAAAT 
ATTTTGGATA CCCAATCGTC CGCTAAAGTA GTATCAAAGA GTGGCGTTCT CTTCTTCGGA 
TTGGTGGGCG ATTCAGCTCT TGGCTGCTGG AACGAACATC GAACACTTGA AAGACACAAT 
ATCCGTACCG TCGCTCAAAG TGATGAGACT CTTCAAATGA TCGCTAGCAT GAAGATTAAG 
GAAGCTCTNC CACACGTGCC TATATTCGAT AGGTATATAA ACCGTGAATA CATATTGGTT 
TTAAGTAACA AAATGCAAAA AATGGTGAAT AATGACTTCA ACTTCGACGA TGTTAACTTC 
AGAATTATGA ACGCGAATGT AAACGAATTG ATATTGAACA CTCGTTGCGA AAATCCCGAT 
AATGATCGAA CACCTTTCAA AATTTCAATC CATTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001011579.1
CDS	46..1344
Translation	MTRLFMLVCLGIVCQGTTGNILRGESLNKSLPILHEWKFFDYDFGSDERRQDAILSGEYDYKNNYPSDIDQWHDKIFVTMLRYNGVPSSLNVISKKVGDGGPLLQPYPDWSFAKYDDCSGIVSASKLAIDKCDRLWVLDSGLVNNTQPMCSPKLLTFDLTTSQLLKQVEIPHDVAVNATTGKGRLSSLAVQSLDCNTNSDTMVYIADEKGEGLIVYHNSDDSFHRLTSNTFDYDPKFTKMTIDGESYTAQDGISGMALSPMTNNLYYSPVASTSLYYVNTEQFRTSDYQQNDIHYEGVQNILDTQSSAKVVSKSGVLFFGLVGDSALGCWNEHRTLERHNIRTVAQSDETLQMIASMKIKEAnullPHVPIFDRYINREYILVLSNKMQKMVNNDFNFDDVNFRIMNANVNELILNTRCENPDNDRTPFKISIHL

Target ORF information:

RefSeq Version	NM_001011579.1
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera major royal jelly protein 1 (Mrjp1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001011579.1

ORF Insert Sequence:

ATGACAAGAT TGTTTATGCT GGTATGCCTT GGCATAGTTT GTCAAGGTAC GACAGGCAAC 
ATTCTTCGAG GAGAGTCTTT AAACAAATCA TTACCCATCC TTCACGAATG GAAATTCTTT 
GATTATGATT TCGGTAGCGA TGAAAGAAGA CAAGATGCAA TTCTATCTGG CGAATACGAC 
TACAAGAATA ATTATCCATC CGACATTGAC CAATGGCATG ATAAGATTTT TGTCACCATG 
CTGAGATACA ATGGCGTACC TTCCTCTTTG AACGTGATAT CTAAAAAGGT CGGTGATGGT 
GGTCCTCTTC TACAACCTTA TCCCGATTGG TCGTTTGCTA AATATGACGA TTGCTCTGGA 
ATCGTGAGCG CCTCAAAACT TGCGATCGAC AAATGCGACA GATTGTGGGT TCTGGACTCA 
GGTCTTGTCA ATAATACTCA ACCCATGTGT TCTCCAAAAC TGCTCACCTT TGATCTGACT 
ACCTCGCAAT TGCTCAAGCA AGTTGAAATA CCACATGATG TTGCCGTAAA TGCCACTACA 
GGAAAGGGAA GATTATCATC TCTAGCTGTT CAATCTTTAG ATTGCAATAC AAATAGCGAT 
ACTATGGTGT ACATAGCAGA CGAGAAAGGT GAAGGTTTAA TCGTGTATCA TAATTCTGAT 
GATTCCTTCC ATCGATTGAC TTCCAACACT TTCGATTACG ATCCTAAATT TACCAAAATG 
ACGATCGATG GAGAAAGTTA CACAGCCCAA GATGGAATTT CTGGAATGGC TCTTAGTCCC 
ATGACTAACA ATCTCTATTA CAGTCCTGTA GCTTCCACCA GTTTGTATTA TGTTAACACG 
GAACAATTCA GAACATCCGA TTATCAACAG AATGACATAC ATTACGAAGG AGTCCAAAAT 
ATTTTGGATA CCCAATCGTC CGCTAAAGTA GTATCAAAGA GTGGCGTTCT CTTCTTCGGA 
TTGGTGGGCG ATTCAGCTCT TGGCTGCTGG AACGAACATC GAACACTTGA AAGACACAAT 
ATCCGTACCG TCGCTCAAAG TGATGAGACT CTTCAAATGA TCGCTAGCAT GAAGATTAAG 
GAAGCTCTNC CACACGTGCC TATATTCGAT AGGTATATAA ACCGTGAATA CATATTGGTT 
TTAAGTAACA AAATGCAAAA AATGGTGAAT AATGACTTCA ACTTCGACGA TGTTAACTTC 
AGAATTATGA ACGCGAATGT AAACGAATTG ATATTGAACA CTCGTTGCGA AAATCCCGAT 
AATGATCGAA CACCTTTCAA AATTTCAATC CATTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Characterizing the Structure and Oligomerization of Major Royal Jelly Protein 1 (MRJP1) by Mass Spectrometry and Complementary Biophysical Tools.
Biochemistry56(11)1645-1655(2017 03)
Mandacaru SC,do Vale LH,Vahidi S,Xiao Y,Skinner OS,Ricart CA,Kelleher NL,de Sousa MV,Konermann L

Finding the missing honey bee genes: lessons learned from a genome upgrade.
BMC genomics1586(2014 Jan)
Elsik CG,Worley KC,Bennett AK,Beye M,Camara F,Childers CP,de Graaf DC,Debyser G,Deng J,Devreese B,Elhaik E,Evans JD,Foster LJ,Graur D,Guigo R,,Hoff KJ,Holder ME,Hudson ME,Hunt GJ,Jiang H,Joshi V,Khetani RS,Kosarev P,Kovar CL,Ma J,Maleszka R,Moritz RF,Munoz-Torres MC,Murphy TD,Muzny DM,Newsham IF,Reese JT,Robertson HM,Robinson GE,Rueppell O,Solovyev V,Stanke M,Stolle E,Tsuruda JM,Vaerenbergh MV,Waterhouse RM,Weaver DB,Whitfield CW,Wu Y,Zdobnov EM,Zhang L,Zhu D,Gibbs RA,

Quantification of major royal jelly protein 1 in fresh royal jelly by indirect enzyme-linked immunosorbent assay.
Bioscience, biotechnology, and biochemistry77(6)1310-2(2013)
Yamaguchi K,He S,Li Z,Murata K,Hitomi N,Mozumi M,Ariga R,Enomoto T

Evolution of the Yellow/Major Royal Jelly Protein family and the emergence of social behavior in honey bees.
Genome research16(11)1385-94(2006 Nov)
Drapeau MD,Albert S,Kucharski R,Prusko C,Maleszka R

Insights into social insects from the genome of the honeybee Apis mellifera.
Nature443(7114)931-49(2006 Oct)