PHRF1 cDNA ORF clone, Apis mellifera(honey bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	PHRF1
Entrez Gene ID	727274
Full Name	PHD and ring finger domains 1
Synonyms	GB13158
Gene Type	protein-coding
Organism	Apis mellifera(honey bee)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001256037.1	NP_001242966.1	PHD and ring finger domains 1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Finding the missing honey bee genes: lessons learned from a genome upgrade.
Elsik CG, Worley KC, Bennett AK, Beye M, Camara F, Childers CP, de Graaf DC, Debyser G, Deng J, Devreese B, Elhaik E, Evans JD, Foster LJ, Graur D, Guigo R, , Hoff KJ, Holder ME, Hudson ME, Hunt GJ, Jiang H, Joshi V, Khetani RS, Kosarev P, Kovar CL, Ma J, Maleszka R, Moritz RF, Munoz-Torres MC, Murphy TD, Muzny DM, Newsham IF, Reese JT, Robertson HM, Robinson GE, Rueppell O, Solovyev V, Stanke M, Stolle E, Tsuruda JM, Vaerenbergh MV, Waterhouse RM, Weaver DB, Whitfield CW, Wu Y, Zdobnov EM, Zhang L, Zhu D, Gibbs RA,
BMC genomics1586(2014 Jan)

Insights into social insects from the genome of the honeybee Apis mellifera.

Nature443(7114)931-49(2006 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PHRF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PHRF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHd00192	NM_001256037.1 Latest version!	Apis mellifera PHD and ring finger domains 1 (PHRF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PHRF1 ( NM_001256037.1 ) cDNA ORF clone, Apis mellifera(honey bee) -> NP_001242966.1 Apis mellifera PHD and ring finger domains 1 (PHRF1), mRNA.

Price & Availability↑

CloneID	OHd00192
Clone ID Related Accession (Same CDS sequence)	NM_001256037.1
Accession Version	NM_001256037.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7455bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-19
Organism	Apis mellifera(honey bee)
Product	PHD and ring finger domains 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AADG06011607.1 and AADG06011608.1. On or before Dec 24, 2011 this sequence version replaced XM_001122984.2, XM_003251781.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00008880, SAMN00113341 [ECO:0000348] ##Evidence-Data-END##

ATGTCTAGTG ACAGTAATCA AACTTCTCAA TCTGCCAAGC GGAAAAAACT TGTTAAACTT 
GATAGCGAAG ATAGTGATGA ATCTATTGTA CAATCTCGTA GAAGAAAAAA GACTTTTAAA 
TTCATTAGTG AAGGTGAAAC TAGTGATGAA AATAGTGATG TTCAAAGACC TATTGTTCAA 
GCACAAAATC ATAGGGTTAT TGCAAAAGAT GAATTAAATT ATGAAAGCAG CAGTTCAAAC 
ACTAGTAAAT ACAAGCAATT TGGAGAAAGT AGTGAAAATT CAGAATGGCA ATCAGATTGG 
AGTAGTTCAT CAGAATTAGA AAAAAAATCT CCTGTCAAAC GAAAAGTTCT TGGCAAACCA 
AAAGCTAAAA AACCACCAAA AATTGTATCA GATACAGATT CAGATAATAA TGATGATCAA 
GTAGAAAAGT GTCCAATTTG TTTACTTCCT TTTAAAAGGC AGCAATTAGG CACTCCTTCT 
TCTTGTGAAC ATTGTTTTTG TTTAGAATGT TTAATTGAAT GGAGTAAAAA TATAAATACT 
TGTCCAGTAG ATCGACAAAC TTTTACAATA ATTCATGTCA GAGATAAACT TGGTGGTCAG 
ATAATAAGAT GTGTTCCAGT TGAAGTTGCT TCCAGTGAAG AAGAAAATTT GGATGATCTT 
ACATTTTGTG AAGTATGCCA TCAAAGTAAT AGAGAAGATC GTATGCTTCT TTGTGATGGT 
TGTGATTGTG GTTATCATTT AGAATGTTTG ACTCCTCCAT TGAATGAAGT ACCTATAGAA 
GAATGGTTTT GCCCAGAATG TTCTCAGAAT AGAGAAATCA ATGCAGAAGT TGTCAAAATT 
GATATGAACG AAATCATAGA TCCAATAGAA GAAGCACGTA GACTTGGTGT TACTTATGGA 
AGAATTCGAA TTAATGCACT AAATTCTTAT CCAAGAATTA TACCTCGTAC TAGACATACA 
GAACGTGTTA GAGCTACTAT TCGCAAAAGA AATAGATTAG ATGATAATAT ACAATTAACT 
CGTCAAATAC CATTTGATCC AAATCAACCT TCTACTTCAT CTGGTTTGGA GTCTTTTGGA 
GATAAAAGTC AGAGTCGATC TCCAAGCAAT GCCTCTAGTT CTTTAGGTTC TGGAGATCGT 
AATTGTTCAA ACAGATATTT TAGAAAATCA CGAAAATATA GAAGTAGTTC TAGTGATTCT 
AGTGATGAAA GTATTTCCAG AAAATCTCGA AAAAGAATTA ATAATAAAAA TAATTCTGAA 
AATTCAAATA ATAATAAATC TAGAAAGAAA AGTACTAATA ATAAACAGTC TGGAGGTAGC 
TCGCGAAAAA ATGAAACTAG AAATAATTCA AGAAAGTCTG GAAAATCTAA AAATGTTTCT 
AGTAAGGAAG CTCTATTAAG AGAAGTTAGA ATTATAGAAT GTAATGAAAA TGGAGAAGAA 
GAAGAAATTA TAACACATGT CAAAGTAGCT TCAATTACAT TAAGAAAGAG AAGAAGAACA 
AAAAGAAAAA AGAGAAGAAA TCGTAGACAT CGAGCTAGAA CTTTATCCAG TGTTTTAACT 
AATACCAAAA AAAGACTTAC GGTTGCTCTG GGTATTTGTA AACCAGTTAA AAATGTCCCT 
AGTTTGTTAC CTAGTAGAAA TCGGCAAATT ATATCAGATA GATCTGCATT AACCATTGCC 
CGATATAATG CTGGAATCTC AAGACTTAGT ATTTTTGGAG AGAGTTTGCA TTTGGATTAT 
ACTCCTCCGG AATCAGATAC AGAAGATTAT CATGCAATTG TTGGTCCTAC TGTAACTGTT 
CAAAGACATT CTATAATTTC TTCTCGAAGA CATTTACGTT TAAAAAATAT TGTTACTCCT 
CAACAACCTC AATCTGATCT TCTAAGTAAT ATAATAAAAG ACCAAGAGAT TTGGCATTCA 
AATAATAATA ACATAACTAT AAAAGCAAAT GGAATTCTTT TGATTAAAGA AAAAGAAATA 
TTGAATGATT TGAAAAAAGG AAAATCCGAA GAAAAAGAAC TATTCAAAAA TGAAGAAGTA 
GAATCAATTG TAGAAAATAG AGTTTCTAGA GTTGATACTT CATTAGAAGT TTCTAGAATT 
GACACTCCAC TAGAAATTTC TAGAATCAAT CCAGAATCAA TTGTACAAGC TCCAATGTAT 
AACAATTCCT GTGGAGGAGG AAATAGAGGC AATTTTAGAG GTAGTTATAG GGATAATACG 
AGGCATAGTC ATGGAGGGCA AAATTATGGA AGAGATTCCT TAGGTGGAAG TGGAAGACAC 
AGTTATGGAG GAAGTGGTGG AAATTTTGGA AGAGATTTTG GACCACCTTC GTTTTATAAT 
CCAAATTTTG AACATTGTGG ACCTCCAATG TTTGGAGGTA ATCCTCCATT CAGAAATCGA 
AATCCTCAAC ATTTTAGAAA CGAAAGATTT CGTTTTATGC CTTCAGGAAG ACCACAATTT 
AATTTTGCTG AAGGTTTATC TGAGCATCAT ACTTTTTCTG AAAGATATAA TCGTCCAAGA 
TCTCCACAAG TTTCTAATGA ACGTCCTCAA AATAATATGC CACCAAATTT ACCAGAAGAT 
ACTCCAAGAA GCGAATCTGT TGAGCAACAA TCACAAGATA TTGAAAATGT CTCTATTAGA 
CAAGAAGAGG ATTGTGATAT TTATTGCGAT ATTGAAACAA AGTCTGATGG AGAACTCCAA 
GATCAACAAA ATAGTTCTAT GGCATTATTA CCACCACCAG AACCTCCATC AGGACTTTTA 
GATTTTGAAG AAAATTCTAA AAGTGATAAA GATAGTGATC AAGAATTAGT TATTGATGAT 
AGTCAAAAAG AAGATACTCC AAAAAATGAT AAATATGACC CTTTTTCTGT GGATAGTGAT 
AGTGATAGTG AAATAAAGAA AAATGAAGGA ATTATGGAAA AAGAGAAAGA AGAACAAATT 
GCAAAAGATA TAGCTCAAGC AAAAACTTCA GATACATCTA TTCGTTTATC AACTTATGAT 
GATGAAGAAG AAGAAGATTC TCAAGCTGAT TGTCCAAATT TCTCTATTTA TTCTTCTCAA 
ACAATTGATG TAGCACGGCA CACTGAACAA CAATTATCTC AACAAATAGG ACCGCTTCAA 
CCACCCCCTC TTCCTCCTTC TATTCCAGAT GATGATGATG TTATTGTTGG TGATGTTCAG 
ACTTGTGATT TAACTGAAAT TCCAGAACCA TCAGATCCAT ATGTTGAAGC TTTGAGAAAA 
GAAAGGCAAA CTTTAAGTAA AATTCCACCA AAGAAAATTT CTCATGATAG TAGAGGAAAA 
ATTACATTTA AAATTGGTAC TAAATTGAAG ATTAATAACA GATTATTAGG ATTACAGGAA 
GAAGAAAAAG AAAGTAATGA TGATAAGAAA AAAGAAATTG AACTTATAAA AGAAGAAGAA 
ACTTCTGTTG ATCGTGAAGA AGAACGAAAA AGAATTACTA AAAACAAATA TGAAGAAGAA 
ATTCAATTAA AAGGAGATAA AGAGCAAAAT ATAAAAGAAA TTCTAATACC TGTAATTCCT 
TTAAATTTGA AAGAAAAAAA CACAAATAAA GAAGAAAAAG AAAAAGAATG GATAGAAAAA 
TCTAAAGATA ATACAGCAGA GAATGATTCA AATGTAATCA TTACTACAGA AGTAACTCAA 
TTATCTCCCA AAAGATTAGA AAATGAAATT ATTAGTGAAT GTCTATCTGA AGAAGGAAGT 
AAAACTTTTG CTTTGCTTCA TAAACCATCA AAAATTGATC TTGGAAAAAA AGATTCAAAA 
AAAGAATCAA AAAATAAAGA AGAATATACA GTAAAAATTA AAAATCCATA TGATTATGAA 
AGCGAAAAAG ATGATAAACA TGATAAAAGA GAAGAAAATG ATGATGATGA TGACGATGAT 
GATGATGATG AAGAAGAAGA GGAAGAAGAA GAAGAAGAGG AGGAGGAGGA AGAAGAAGAC 
GAAGAAGAAG AAGAAGAAGA AGAAAGATTT CCTATAGAAA AAGAAAAATC TAGCAAAAAA 
AATATAATTG AGAAAGATAT TGATAAAAAC TCATGGGAAT CTGATGGAGC TTATACTCCT 
TGCAAAGATG AACTTCCCTT AAAAGAATCA AAATCTTCTT CCGAACAATT GCCATCTATT 
GAAAGTGGAT TAGAGCCAAT TACACCAACA AAAGATAAAC CTGATGATTT AGATAGTTGT 
AGGACTCCAG ATTATAATGT TTTAGGCACA GAAGCTATCT CAGAAACAGA TGAAGCAATG 
AATTTTGAAG ATGAGTTAAC TTTATTGAGT TTACGAAAGG AAAAAGAAAT GGAAGATGGA 
GAAATCATTG AAGAAAAAAT TATAAAGGAA GGAGATAAAA TTGAAAAAGA AAAAGAAGAG 
GAAAAGAAAC GAAAGAAAAA GGAAAAGAAA GAAAAAAGTA AAGAAACAGA AAAAAATAAA 
GAAAATATTG CTTCAGAAAA TCAAGTAGCA TGGAAAAAAC TTTCAAAAAA TACAAAAGAA 
AGATCTTATA GAGACAAAGA AAAGAGATCA AAATCGAAGG AAAGAGAAAA ATTAAAGAAA 
AAGAAAGAAG TAGTAAAAAA GAAGGAAAAA AGAAAAGAAT TACCAAGATA TGACGTTAGA 
AAAATTGTTG CAGAAAGACC TTCAAGACCT AGAAAAGATG AGTATGGTAG AGATATTAGA 
GAAAAATCAA GACAAAGAAG TAAATCTAGA AGTTGTAATT CATCAAAACG TTCTAGATCA 
TATTCTAAGA TTCGCTCTAG AAGTAGATCC AGAACTAGAT TACAAAGATC ATGGTCGAGA 
GATCGTAGAT CTTATTCTAA ATCTCTTTCC AGACGAAGAT CTCTTTCTCG TGGAAGGCGT 
AAATCACCTA AAAGAAGATC AACTTCTAGA AGAAGATCTT CTTCGAGAAA ACGATCCCTT 
TCTAGAAGAA GATCAAGATC TGTTTCTAGA AAACGAAGAT CTTTATCGAG AAGAAGATCC 
AGGCGATCAA TCTCGAGATC TAGACAAAGA AGATCTATAT CACGATCTCG AAGATCACTA 
TCTAGATCTC GCGATAGACG TAAAGATAAG AAAAAGTACA AATCTCGAAG TCGATCGAGA 
AACAGAAAAA AATCTCGATC GAAATCACCT AGAAAGTCTT CGAAATCCAG AGAAAGAAAA 
CATTCAAAGA GAAAAGCACA AAGTCGATCA AGATCAAAAG GAAGATCTTG TTCCAGAAAT 
TGGGAACAAG TTGATAAAAT TGTAGAACAA GAATTTTCTA GAGAAAGAGA AGAAAGAGAA 
TCTTGGAGTG CACAATGGAC ACCATCTTGG TCAAGATCTA GATCAAAAAC ACCACAACAC 
GTTCAACAAG AACCAATTAC TTCTAGAGAA TGGTCTCCAA TGTTAGATAA TGTTTCGGTT 
CCGCCAAAAA ATCTAACTGT AATTTTAACA AATAAGGAAG CAATTAAAAA AAAGAAGAAA 
GAACGTAGAA AAGAAAGCAA AAAAACAAAA GATGAAAAAA AGAGAAAAGG AAAAAGGAAT 
AGGACTCCAC CACCATCAAA AGAGGTTTTT GCTAGTGGAG ATAATATTTT GGTTAGTGTA 
TGTTTCAACA AAGATAATGA AAATGATCAA ACAGTTGTTC CAGAACTTCC AGAGACAATT 
CCTCTAATTT CTCCTCTAAA ACGTCGCCGT AGAGAATCTC TTCAAACTGA TCCTCCAAAA 
CGTTCAAAAA AAGAAAAGTC AAAAGATAAA CGTTCTAAAT CTCCAAAGCC AAAAAAAGAT 
AAAAAGAAAA AATCAAAAGC AGCTGAAATA GCGGCTACAA AAAAACCAGT CGCAGTAATT 
GATTTAGATC AATCTCCATT TCGGGAACAG ACACCATCTC CTAGAGACGT TATAGTTCTT 
AGTGATGATG ATGATAAACA AATTCAACAA ATCACATCAG ATCAATGTCA ACCTTCTCAA 
AATTCTCCAC CTAGAGAACA GTTTATTTCT CAGGGTCCAA AAACACCTCC GGAACCACAA 
ATAAAATTTT CAATAAATAA ACAAAGCAGT AATTTACGAC CATCAATGTT AAATCCGCTT 
TTAGATGAAG AAGAAGAAGA AGAAATGGAT GAAAGAGTCG AAGAAGAATT GGAAATGAGA 
GCACAAGAAG AATTAGAATT AAGATTAAAA ATTGGACCAA ATACCCCTCC AGAACCTCCC 
ACATCTCCAC CAACAAGTCC AGATGCTTAT GATCCATTTG ATCCAACTAA ATCTAGATCG 
CCAACACCTG ATGTTAGTCA GGAAGTGAAT TCCAGCGATG AAAGAAGAAG ATCTCCAAGA 
AAACATGATT CTGTTGATGA TAGTATTCTT GAAGAGGAAA ATCAAAATCA AGAAAGGCAA 
AGTCAAGAAA AGCAACTAGA AAAGCCTAAA ATCATTTCTA TGGTTACAAT CAAGAGAGCA 
TCTCCACAAA GAGATGATGT TAATGATAAT GATAACAATA ATGATATTAC AGTCTCAGCC 
GGAAATCTAG AAATACAACA AAATCAACAA AATGTGCAAA ACCAGAATAA TCCTTTCGCA 
GTAATGAATC CTGTGCTAGC TACAGTAGCT GCAGCAGTAC AGAGAAGTGG AGTATTTAAT 
GCACCAAATT CAAATAATAA TCAACGTAAC TTACAATCAA ATCGAATTTC ACCAACCAAT 
CAACAAAAAC AACGTTCTGG CGATCGTTCA CAGGCACCTC CAGTTTTTAC GAATTCAGGA 
AAATCTTCAA GAAGCTCGAA ATCCGGACAA ACAAAAACAA ATGGACAAAA TGGAAATGAT 
GCCGGAACAG AAACAACGGA TAATGTAGAT ATGTCATCTC CTTATTCTCC TGGTTCGACG 
CTTAGCGATG GAATATTCGA TCCACCTAGT CCTACAAATT ATAATTCACC TAATACTGGG 
ACAGGAAGTG GAGCAGGACC TATTGGAACT CAGATTACAA AAAATAGTAA TTCGAAAACA 
ATAAAAACCA CAGAAAAAAA AGATGCTTTC GATGCTTTAT TTGGTTCTTT ACCGATTACA 
AAAACTAGCA CTAAATCGAA TAGAAATAAA AAATCTGAAA AAGATAAAAA GAAAAAATCT 
ACAAATCCAA AAGTTGGTGT TAGAATGGAC GAAAATCAGC TTCAAATATT AGATGATCTT 
CCAAGTTCTG CAGTTGAAAT GCAAGTCAAG GATAAGTTCC TAAAGAAATT AAATCGTCAA 
GAACGAGTGG TTGAAGAAGT GAAGTTAGTT TTAAAACCAC ATTACACAAA AAAACATGTC 
ACAAAAGAAG AGTACAAGGA TATTATGCGG AAAGCAGTAC CTAAGATATG TCATAATAAA 
ACGGGAGAAA TCAATCCGAA AAAAATTGCC CATTTAATAG AAGCTTATGT CAAAAAATGC 
AGAAATAGCA AAAAGAAAAC TATTGAAAGT TCCTCCACAA AGGTCTCTAA ACCAGCAAAA 
ATTTTGTGGA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001242966.1
CDS	228..7682
Translation	MSSDSNQTSQSAKRKKLVKLDSEDSDESIVQSRRRKKTFKFISEGETSDENSDVQRPIVQAQNHRVIAKDELNYESSSSNTSKYKQFGESSENSEWQSDWSSSSELEKKSPVKRKVLGKPKAKKPPKIVSDTDSDNNDDQVEKCPICLLPFKRQQLGTPSSCEHCFCLECLIEWSKNINTCPVDRQTFTIIHVRDKLGGQIIRCVPVEVASSEEENLDDLTFCEVCHQSNREDRMLLCDGCDCGYHLECLTPPLNEVPIEEWFCPECSQNREINAEVVKIDMNEIIDPIEEARRLGVTYGRIRINALNSYPRIIPRTRHTERVRATIRKRNRLDDNIQLTRQIPFDPNQPSTSSGLESFGDKSQSRSPSNASSSLGSGDRNCSNRYFRKSRKYRSSSSDSSDESISRKSRKRINNKNNSENSNNNKSRKKSTNNKQSGGSSRKNETRNNSRKSGKSKNVSSKEALLREVRIIECNENGEEEEIITHVKVASITLRKRRRTKRKKRRNRRHRARTLSSVLTNTKKRLTVALGICKPVKNVPSLLPSRNRQIISDRSALTIARYNAGISRLSIFGESLHLDYTPPESDTEDYHAIVGPTVTVQRHSIISSRRHLRLKNIVTPQQPQSDLLSNIIKDQEIWHSNNNNITIKANGILLIKEKEILNDLKKGKSEEKELFKNEEVESIVENRVSRVDTSLEVSRIDTPLEISRINPESIVQAPMYNNSCGGGNRGNFRGSYRDNTRHSHGGQNYGRDSLGGSGRHSYGGSGGNFGRDFGPPSFYNPNFEHCGPPMFGGNPPFRNRNPQHFRNERFRFMPSGRPQFNFAEGLSEHHTFSERYNRPRSPQVSNERPQNNMPPNLPEDTPRSESVEQQSQDIENVSIRQEEDCDIYCDIETKSDGELQDQQNSSMALLPPPEPPSGLLDFEENSKSDKDSDQELVIDDSQKEDTPKNDKYDPFSVDSDSDSEIKKNEGIMEKEKEEQIAKDIAQAKTSDTSIRLSTYDDEEEEDSQADCPNFSIYSSQTIDVARHTEQQLSQQIGPLQPPPLPPSIPDDDDVIVGDVQTCDLTEIPEPSDPYVEALRKERQTLSKIPPKKISHDSRGKITFKIGTKLKINNRLLGLQEEEKESNDDKKKEIELIKEEETSVDREEERKRITKNKYEEEIQLKGDKEQNIKEILIPVIPLNLKEKNTNKEEKEKEWIEKSKDNTAENDSNVIITTEVTQLSPKRLENEIISECLSEEGSKTFALLHKPSKIDLGKKDSKKESKNKEEYTVKIKNPYDYESEKDDKHDKREENDDDDDDDDDDEEEEEEEEEEEEEEEEDEEEEEEEERFPIEKEKSSKKNIIEKDIDKNSWESDGAYTPCKDELPLKESKSSSEQLPSIESGLEPITPTKDKPDDLDSCRTPDYNVLGTEAISETDEAMNFEDELTLLSLRKEKEMEDGEIIEEKIIKEGDKIEKEKEEEKKRKKKEKKEKSKETEKNKENIASENQVAWKKLSKNTKERSYRDKEKRSKSKEREKLKKKKEVVKKKEKRKELPRYDVRKIVAERPSRPRKDEYGRDIREKSRQRSKSRSCNSSKRSRSYSKIRSRSRSRTRLQRSWSRDRRSYSKSLSRRRSLSRGRRKSPKRRSTSRRRSSSRKRSLSRRRSRSVSRKRRSLSRRRSRRSISRSRQRRSISRSRRSLSRSRDRRKDKKKYKSRSRSRNRKKSRSKSPRKSSKSRERKHSKRKAQSRSRSKGRSCSRNWEQVDKIVEQEFSREREERESWSAQWTPSWSRSRSKTPQHVQQEPITSREWSPMLDNVSVPPKNLTVILTNKEAIKKKKKERRKESKKTKDEKKRKGKRNRTPPPSKEVFASGDNILVSVCFNKDNENDQTVVPELPETIPLISPLKRRRRESLQTDPPKRSKKEKSKDKRSKSPKPKKDKKKKSKAAEIAATKKPVAVIDLDQSPFREQTPSPRDVIVLSDDDDKQIQQITSDQCQPSQNSPPREQFISQGPKTPPEPQIKFSINKQSSNLRPSMLNPLLDEEEEEEMDERVEEELEMRAQEELELRLKIGPNTPPEPPTSPPTSPDAYDPFDPTKSRSPTPDVSQEVNSSDERRRSPRKHDSVDDSILEEENQNQERQSQEKQLEKPKIISMVTIKRASPQRDDVNDNDNNNDITVSAGNLEIQQNQQNVQNQNNPFAVMNPVLATVAAAVQRSGVFNAPNSNNNQRNLQSNRISPTNQQKQRSGDRSQAPPVFTNSGKSSRSSKSGQTKTNGQNGNDAGTETTDNVDMSSPYSPGSTLSDGIFDPPSPTNYNSPNTGTGSGAGPIGTQITKNSNSKTIKTTEKKDAFDALFGSLPITKTSTKSNRNKKSEKDKKKKSTNPKVGVRMDENQLQILDDLPSSAVEMQVKDKFLKKLNRQERVVEEVKLVLKPHYTKKHVTKEEYKDIMRKAVPKICHNKTGEINPKKIAHLIEAYVKKCRNSKKKTIESSSTKVSKPAKILWS

Target ORF information:

RefSeq Version	NM_001256037.1
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera PHD and ring finger domains 1 (PHRF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256037.1

ORF Insert Sequence:

ATGTCTAGTG ACAGTAATCA AACTTCTCAA TCTGCCAAGC GGAAAAAACT TGTTAAACTT 
GATAGCGAAG ATAGTGATGA ATCTATTGTA CAATCTCGTA GAAGAAAAAA GACTTTTAAA 
TTCATTAGTG AAGGTGAAAC TAGTGATGAA AATAGTGATG TTCAAAGACC TATTGTTCAA 
GCACAAAATC ATAGGGTTAT TGCAAAAGAT GAATTAAATT ATGAAAGCAG CAGTTCAAAC 
ACTAGTAAAT ACAAGCAATT TGGAGAAAGT AGTGAAAATT CAGAATGGCA ATCAGATTGG 
AGTAGTTCAT CAGAATTAGA AAAAAAATCT CCTGTCAAAC GAAAAGTTCT TGGCAAACCA 
AAAGCTAAAA AACCACCAAA AATTGTATCA GATACAGATT CAGATAATAA TGATGATCAA 
GTAGAAAAGT GTCCAATTTG TTTACTTCCT TTTAAAAGGC AGCAATTAGG CACTCCTTCT 
TCTTGTGAAC ATTGTTTTTG TTTAGAATGT TTAATTGAAT GGAGTAAAAA TATAAATACT 
TGTCCAGTAG ATCGACAAAC TTTTACAATA ATTCATGTCA GAGATAAACT TGGTGGTCAG 
ATAATAAGAT GTGTTCCAGT TGAAGTTGCT TCCAGTGAAG AAGAAAATTT GGATGATCTT 
ACATTTTGTG AAGTATGCCA TCAAAGTAAT AGAGAAGATC GTATGCTTCT TTGTGATGGT 
TGTGATTGTG GTTATCATTT AGAATGTTTG ACTCCTCCAT TGAATGAAGT ACCTATAGAA 
GAATGGTTTT GCCCAGAATG TTCTCAGAAT AGAGAAATCA ATGCAGAAGT TGTCAAAATT 
GATATGAACG AAATCATAGA TCCAATAGAA GAAGCACGTA GACTTGGTGT TACTTATGGA 
AGAATTCGAA TTAATGCACT AAATTCTTAT CCAAGAATTA TACCTCGTAC TAGACATACA 
GAACGTGTTA GAGCTACTAT TCGCAAAAGA AATAGATTAG ATGATAATAT ACAATTAACT 
CGTCAAATAC CATTTGATCC AAATCAACCT TCTACTTCAT CTGGTTTGGA GTCTTTTGGA 
GATAAAAGTC AGAGTCGATC TCCAAGCAAT GCCTCTAGTT CTTTAGGTTC TGGAGATCGT 
AATTGTTCAA ACAGATATTT TAGAAAATCA CGAAAATATA GAAGTAGTTC TAGTGATTCT 
AGTGATGAAA GTATTTCCAG AAAATCTCGA AAAAGAATTA ATAATAAAAA TAATTCTGAA 
AATTCAAATA ATAATAAATC TAGAAAGAAA AGTACTAATA ATAAACAGTC TGGAGGTAGC 
TCGCGAAAAA ATGAAACTAG AAATAATTCA AGAAAGTCTG GAAAATCTAA AAATGTTTCT 
AGTAAGGAAG CTCTATTAAG AGAAGTTAGA ATTATAGAAT GTAATGAAAA TGGAGAAGAA 
GAAGAAATTA TAACACATGT CAAAGTAGCT TCAATTACAT TAAGAAAGAG AAGAAGAACA 
AAAAGAAAAA AGAGAAGAAA TCGTAGACAT CGAGCTAGAA CTTTATCCAG TGTTTTAACT 
AATACCAAAA AAAGACTTAC GGTTGCTCTG GGTATTTGTA AACCAGTTAA AAATGTCCCT 
AGTTTGTTAC CTAGTAGAAA TCGGCAAATT ATATCAGATA GATCTGCATT AACCATTGCC 
CGATATAATG CTGGAATCTC AAGACTTAGT ATTTTTGGAG AGAGTTTGCA TTTGGATTAT 
ACTCCTCCGG AATCAGATAC AGAAGATTAT CATGCAATTG TTGGTCCTAC TGTAACTGTT 
CAAAGACATT CTATAATTTC TTCTCGAAGA CATTTACGTT TAAAAAATAT TGTTACTCCT 
CAACAACCTC AATCTGATCT TCTAAGTAAT ATAATAAAAG ACCAAGAGAT TTGGCATTCA 
AATAATAATA ACATAACTAT AAAAGCAAAT GGAATTCTTT TGATTAAAGA AAAAGAAATA 
TTGAATGATT TGAAAAAAGG AAAATCCGAA GAAAAAGAAC TATTCAAAAA TGAAGAAGTA 
GAATCAATTG TAGAAAATAG AGTTTCTAGA GTTGATACTT CATTAGAAGT TTCTAGAATT 
GACACTCCAC TAGAAATTTC TAGAATCAAT CCAGAATCAA TTGTACAAGC TCCAATGTAT 
AACAATTCCT GTGGAGGAGG AAATAGAGGC AATTTTAGAG GTAGTTATAG GGATAATACG 
AGGCATAGTC ATGGAGGGCA AAATTATGGA AGAGATTCCT TAGGTGGAAG TGGAAGACAC 
AGTTATGGAG GAAGTGGTGG AAATTTTGGA AGAGATTTTG GACCACCTTC GTTTTATAAT 
CCAAATTTTG AACATTGTGG ACCTCCAATG TTTGGAGGTA ATCCTCCATT CAGAAATCGA 
AATCCTCAAC ATTTTAGAAA CGAAAGATTT CGTTTTATGC CTTCAGGAAG ACCACAATTT 
AATTTTGCTG AAGGTTTATC TGAGCATCAT ACTTTTTCTG AAAGATATAA TCGTCCAAGA 
TCTCCACAAG TTTCTAATGA ACGTCCTCAA AATAATATGC CACCAAATTT ACCAGAAGAT 
ACTCCAAGAA GCGAATCTGT TGAGCAACAA TCACAAGATA TTGAAAATGT CTCTATTAGA 
CAAGAAGAGG ATTGTGATAT TTATTGCGAT ATTGAAACAA AGTCTGATGG AGAACTCCAA 
GATCAACAAA ATAGTTCTAT GGCATTATTA CCACCACCAG AACCTCCATC AGGACTTTTA 
GATTTTGAAG AAAATTCTAA AAGTGATAAA GATAGTGATC AAGAATTAGT TATTGATGAT 
AGTCAAAAAG AAGATACTCC AAAAAATGAT AAATATGACC CTTTTTCTGT GGATAGTGAT 
AGTGATAGTG AAATAAAGAA AAATGAAGGA ATTATGGAAA AAGAGAAAGA AGAACAAATT 
GCAAAAGATA TAGCTCAAGC AAAAACTTCA GATACATCTA TTCGTTTATC AACTTATGAT 
GATGAAGAAG AAGAAGATTC TCAAGCTGAT TGTCCAAATT TCTCTATTTA TTCTTCTCAA 
ACAATTGATG TAGCACGGCA CACTGAACAA CAATTATCTC AACAAATAGG ACCGCTTCAA 
CCACCCCCTC TTCCTCCTTC TATTCCAGAT GATGATGATG TTATTGTTGG TGATGTTCAG 
ACTTGTGATT TAACTGAAAT TCCAGAACCA TCAGATCCAT ATGTTGAAGC TTTGAGAAAA 
GAAAGGCAAA CTTTAAGTAA AATTCCACCA AAGAAAATTT CTCATGATAG TAGAGGAAAA 
ATTACATTTA AAATTGGTAC TAAATTGAAG ATTAATAACA GATTATTAGG ATTACAGGAA 
GAAGAAAAAG AAAGTAATGA TGATAAGAAA AAAGAAATTG AACTTATAAA AGAAGAAGAA 
ACTTCTGTTG ATCGTGAAGA AGAACGAAAA AGAATTACTA AAAACAAATA TGAAGAAGAA 
ATTCAATTAA AAGGAGATAA AGAGCAAAAT ATAAAAGAAA TTCTAATACC TGTAATTCCT 
TTAAATTTGA AAGAAAAAAA CACAAATAAA GAAGAAAAAG AAAAAGAATG GATAGAAAAA 
TCTAAAGATA ATACAGCAGA GAATGATTCA AATGTAATCA TTACTACAGA AGTAACTCAA 
TTATCTCCCA AAAGATTAGA AAATGAAATT ATTAGTGAAT GTCTATCTGA AGAAGGAAGT 
AAAACTTTTG CTTTGCTTCA TAAACCATCA AAAATTGATC TTGGAAAAAA AGATTCAAAA 
AAAGAATCAA AAAATAAAGA AGAATATACA GTAAAAATTA AAAATCCATA TGATTATGAA 
AGCGAAAAAG ATGATAAACA TGATAAAAGA GAAGAAAATG ATGATGATGA TGACGATGAT 
GATGATGATG AAGAAGAAGA GGAAGAAGAA GAAGAAGAGG AGGAGGAGGA AGAAGAAGAC 
GAAGAAGAAG AAGAAGAAGA AGAAAGATTT CCTATAGAAA AAGAAAAATC TAGCAAAAAA 
AATATAATTG AGAAAGATAT TGATAAAAAC TCATGGGAAT CTGATGGAGC TTATACTCCT 
TGCAAAGATG AACTTCCCTT AAAAGAATCA AAATCTTCTT CCGAACAATT GCCATCTATT 
GAAAGTGGAT TAGAGCCAAT TACACCAACA AAAGATAAAC CTGATGATTT AGATAGTTGT 
AGGACTCCAG ATTATAATGT TTTAGGCACA GAAGCTATCT CAGAAACAGA TGAAGCAATG 
AATTTTGAAG ATGAGTTAAC TTTATTGAGT TTACGAAAGG AAAAAGAAAT GGAAGATGGA 
GAAATCATTG AAGAAAAAAT TATAAAGGAA GGAGATAAAA TTGAAAAAGA AAAAGAAGAG 
GAAAAGAAAC GAAAGAAAAA GGAAAAGAAA GAAAAAAGTA AAGAAACAGA AAAAAATAAA 
GAAAATATTG CTTCAGAAAA TCAAGTAGCA TGGAAAAAAC TTTCAAAAAA TACAAAAGAA 
AGATCTTATA GAGACAAAGA AAAGAGATCA AAATCGAAGG AAAGAGAAAA ATTAAAGAAA 
AAGAAAGAAG TAGTAAAAAA GAAGGAAAAA AGAAAAGAAT TACCAAGATA TGACGTTAGA 
AAAATTGTTG CAGAAAGACC TTCAAGACCT AGAAAAGATG AGTATGGTAG AGATATTAGA 
GAAAAATCAA GACAAAGAAG TAAATCTAGA AGTTGTAATT CATCAAAACG TTCTAGATCA 
TATTCTAAGA TTCGCTCTAG AAGTAGATCC AGAACTAGAT TACAAAGATC ATGGTCGAGA 
GATCGTAGAT CTTATTCTAA ATCTCTTTCC AGACGAAGAT CTCTTTCTCG TGGAAGGCGT 
AAATCACCTA AAAGAAGATC AACTTCTAGA AGAAGATCTT CTTCGAGAAA ACGATCCCTT 
TCTAGAAGAA GATCAAGATC TGTTTCTAGA AAACGAAGAT CTTTATCGAG AAGAAGATCC 
AGGCGATCAA TCTCGAGATC TAGACAAAGA AGATCTATAT CACGATCTCG AAGATCACTA 
TCTAGATCTC GCGATAGACG TAAAGATAAG AAAAAGTACA AATCTCGAAG TCGATCGAGA 
AACAGAAAAA AATCTCGATC GAAATCACCT AGAAAGTCTT CGAAATCCAG AGAAAGAAAA 
CATTCAAAGA GAAAAGCACA AAGTCGATCA AGATCAAAAG GAAGATCTTG TTCCAGAAAT 
TGGGAACAAG TTGATAAAAT TGTAGAACAA GAATTTTCTA GAGAAAGAGA AGAAAGAGAA 
TCTTGGAGTG CACAATGGAC ACCATCTTGG TCAAGATCTA GATCAAAAAC ACCACAACAC 
GTTCAACAAG AACCAATTAC TTCTAGAGAA TGGTCTCCAA TGTTAGATAA TGTTTCGGTT 
CCGCCAAAAA ATCTAACTGT AATTTTAACA AATAAGGAAG CAATTAAAAA AAAGAAGAAA 
GAACGTAGAA AAGAAAGCAA AAAAACAAAA GATGAAAAAA AGAGAAAAGG AAAAAGGAAT 
AGGACTCCAC CACCATCAAA AGAGGTTTTT GCTAGTGGAG ATAATATTTT GGTTAGTGTA 
TGTTTCAACA AAGATAATGA AAATGATCAA ACAGTTGTTC CAGAACTTCC AGAGACAATT 
CCTCTAATTT CTCCTCTAAA ACGTCGCCGT AGAGAATCTC TTCAAACTGA TCCTCCAAAA 
CGTTCAAAAA AAGAAAAGTC AAAAGATAAA CGTTCTAAAT CTCCAAAGCC AAAAAAAGAT 
AAAAAGAAAA AATCAAAAGC AGCTGAAATA GCGGCTACAA AAAAACCAGT CGCAGTAATT 
GATTTAGATC AATCTCCATT TCGGGAACAG ACACCATCTC CTAGAGACGT TATAGTTCTT 
AGTGATGATG ATGATAAACA AATTCAACAA ATCACATCAG ATCAATGTCA ACCTTCTCAA 
AATTCTCCAC CTAGAGAACA GTTTATTTCT CAGGGTCCAA AAACACCTCC GGAACCACAA 
ATAAAATTTT CAATAAATAA ACAAAGCAGT AATTTACGAC CATCAATGTT AAATCCGCTT 
TTAGATGAAG AAGAAGAAGA AGAAATGGAT GAAAGAGTCG AAGAAGAATT GGAAATGAGA 
GCACAAGAAG AATTAGAATT AAGATTAAAA ATTGGACCAA ATACCCCTCC AGAACCTCCC 
ACATCTCCAC CAACAAGTCC AGATGCTTAT GATCCATTTG ATCCAACTAA ATCTAGATCG 
CCAACACCTG ATGTTAGTCA GGAAGTGAAT TCCAGCGATG AAAGAAGAAG ATCTCCAAGA 
AAACATGATT CTGTTGATGA TAGTATTCTT GAAGAGGAAA ATCAAAATCA AGAAAGGCAA 
AGTCAAGAAA AGCAACTAGA AAAGCCTAAA ATCATTTCTA TGGTTACAAT CAAGAGAGCA 
TCTCCACAAA GAGATGATGT TAATGATAAT GATAACAATA ATGATATTAC AGTCTCAGCC 
GGAAATCTAG AAATACAACA AAATCAACAA AATGTGCAAA ACCAGAATAA TCCTTTCGCA 
GTAATGAATC CTGTGCTAGC TACAGTAGCT GCAGCAGTAC AGAGAAGTGG AGTATTTAAT 
GCACCAAATT CAAATAATAA TCAACGTAAC TTACAATCAA ATCGAATTTC ACCAACCAAT 
CAACAAAAAC AACGTTCTGG CGATCGTTCA CAGGCACCTC CAGTTTTTAC GAATTCAGGA 
AAATCTTCAA GAAGCTCGAA ATCCGGACAA ACAAAAACAA ATGGACAAAA TGGAAATGAT 
GCCGGAACAG AAACAACGGA TAATGTAGAT ATGTCATCTC CTTATTCTCC TGGTTCGACG 
CTTAGCGATG GAATATTCGA TCCACCTAGT CCTACAAATT ATAATTCACC TAATACTGGG 
ACAGGAAGTG GAGCAGGACC TATTGGAACT CAGATTACAA AAAATAGTAA TTCGAAAACA 
ATAAAAACCA CAGAAAAAAA AGATGCTTTC GATGCTTTAT TTGGTTCTTT ACCGATTACA 
AAAACTAGCA CTAAATCGAA TAGAAATAAA AAATCTGAAA AAGATAAAAA GAAAAAATCT 
ACAAATCCAA AAGTTGGTGT TAGAATGGAC GAAAATCAGC TTCAAATATT AGATGATCTT 
CCAAGTTCTG CAGTTGAAAT GCAAGTCAAG GATAAGTTCC TAAAGAAATT AAATCGTCAA 
GAACGAGTGG TTGAAGAAGT GAAGTTAGTT TTAAAACCAC ATTACACAAA AAAACATGTC 
ACAAAAGAAG AGTACAAGGA TATTATGCGG AAAGCAGTAC CTAAGATATG TCATAATAAA 
ACGGGAGAAA TCAATCCGAA AAAAATTGCC CATTTAATAG AAGCTTATGT CAAAAAATGC 
AGAAATAGCA AAAAGAAAAC TATTGAAAGT TCCTCCACAA AGGTCTCTAA ACCAGCAAAA 
ATTTTGTGGA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Finding the missing honey bee genes: lessons learned from a genome upgrade.
BMC genomics1586(2014 Jan)
Elsik CG,Worley KC,Bennett AK,Beye M,Camara F,Childers CP,de Graaf DC,Debyser G,Deng J,Devreese B,Elhaik E,Evans JD,Foster LJ,Graur D,Guigo R,,Hoff KJ,Holder ME,Hudson ME,Hunt GJ,Jiang H,Joshi V,Khetani RS,Kosarev P,Kovar CL,Ma J,Maleszka R,Moritz RF,Munoz-Torres MC,Murphy TD,Muzny DM,Newsham IF,Reese JT,Robertson HM,Robinson GE,Rueppell O,Solovyev V,Stanke M,Stolle E,Tsuruda JM,Vaerenbergh MV,Waterhouse RM,Weaver DB,Whitfield CW,Wu Y,Zdobnov EM,Zhang L,Zhu D,Gibbs RA,

Insights into social insects from the genome of the honeybee Apis mellifera.
Nature443(7114)931-49(2006 Oct)