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AKT1 cDNA ORF clone, Bos taurus(cattle)

Summary

Gene Symbol AKT1
Entrez Gene ID 280991
Full Name AKT serine/threonine kinase 1
Synonyms AKT
General protein information
Preferred Names

AKT serine/threonine kinase 1

Names

RAC-alpha serine/threonine-protein kinase
PKB alpha
RAC-PK-alpha
protein kinase B alpha
Gene Type protein-coding
Organism Bos taurus(cattle)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_173986.2 NP_776411.1 RAC-alpha serine/threonine-protein kinase
XM_024981593.1 XP_024837361.1 RAC-alpha serine/threonine-protein kinase isoform X2
XM_024981592.1 XP_024837360.1 RAC-alpha serine/threonine-protein kinase isoform X1

Pathways from BioSystems


Homologies


Bos taurus (cattle) AKT1 NP_776411.1
Caenorhabditis elegans (roundworm) akt-2 NP_510357.3
Homo sapiens (human) AKT1 NP_001014431.1
Canis lupus familiaris (dog) AKT1 XP_548000.3
Mus musculus (house mouse) Akt1 NP_033782.1
Gallus gallus (chicken) AKT1 NP_990386.1
Pan troglodytes (chimpanzee) AKT1 XP_001143158.2
AKT1 XP_001085265.2
Rattus norvegicus (Norway rat) Akt1 NP_150233.1
Danio rerio (zebrafish) LOC100331515 XP_003200336.2
Caenorhabditis elegans (roundworm) akt-1 NP_001023646.1
Xenopus tropicalis (tropical clawed frog) akt1 XP_004917249.1

Gene Ontology


Bibliography

Related articles in PubMed

Prostaglandin F2?-induced luteolysis involves activation of Signal transducer and activator of transcription 3 and inhibition of AKT signaling in cattle.
Rovani MT, Ilha GF, Gasperin BG, N?brega JE Jr, Siddappa D, Glanzner WG, Antoniazzi AQ, Bordignon V, Duggavathi R, Gon?alves PBD
Molecular reproduction and development84(6)486-494(2017 Jun)

The role of the PI3K-Akt signaling pathway in the developmental competence of bovine oocytes.
Andrade GM, da Silveira JC, Perrini C, Del Collado M, Gebremedhn S, Tesfaye D, Meirelles FV, Perecin F
PloS one12(9)e0185045(2017)

Protein kinase B is required for follicle-stimulating hormone mediated beta-catenin accumulation and estradiol production in granulosa cells of cattle.
G?mez BI, Gifford CA, Hallford DM, Hernandez Gifford JA
Animal reproduction science16397-104(2015 Dec)

Caveolin-1 scaffolding domain residue phenylalanine 92 modulates Akt signaling.
Trane AE, Hiob MA, Uy T, Pavlov D, Bernatchez P
European journal of pharmacology76646-55(2015 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the measurement of levels of PI3K-Akt pathway components in FCs from ovarian follicles carrying oocytes with distinct developmental competences is a useful tool to identify putative molecular pathways involved in the acquisition of oocyte competence.
Title: The role of the PI3K-Akt signaling pathway in the developmental competence of bovine oocytes.

These results demonstrate that activation of AKT is required for gonadotropin regulation of CTNNB1 accumulation and subsequent ovarian E2 production.
Title: Protein kinase B is required for follicle-stimulating hormone mediated beta-catenin accumulation and estradiol production in granulosa cells of cattle.

Caveolin-1 scaffolding domain residue phenylalanine 92 modulates Akt signaling
Title: Caveolin-1 scaffolding domain residue phenylalanine 92 modulates Akt signaling.

TG2 contributes to 5-hydroxytryptamine-induced distal pulmonary artery smooth muscle cell proliferation via promotion of AKT signaling, likely via its serotonylation.
Title: Tissue transglutaminase promotes serotonin-induced AKT signaling and mitogenesis in pulmonary vascular smooth muscle cells.

results suggest that PI3K-Akt activity is important for the internalization of S. aureus and phosphorylation of GSK-3alpha, GSK-3beta, and NF-kappaB.
Title: The phosphoinositide-3-kinase-Akt signaling pathway is important for Staphylococcus aureus internalization by endothelial cells.

The following AKT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AKT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa41644 NM_173986.2
Latest version!		 Bos taurus AKT serine/threonine kinase 1 (AKT1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OBa228205 XM_024981592.1
Latest version!		 Bos taurus AKT serine/threonine kinase 1 (AKT1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OBa228206 XM_024981593.1
Latest version!		 Bos taurus AKT serine/threonine kinase 1 (AKT1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OBa41644
Clone ID Related Accession (Same CDS sequence) NM_173986.2
Accession Version NM_173986.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1443bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-24
Organism Bos taurus(cattle)
Product RAC-alpha serine/threonine-protein kinase
Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X61036.1. On Jun 3, 2003 this sequence version replaced NM_173986.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X61036.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGAACGACG TGGCCATCGT GAAGGAGGGC TGGCTGCACA AGCGAGGTGA GTACATCAAG 
ACGTGGCGGC CGCGTTACTT CCTCCTGAAG AACGATGGCA CGTTCATTGG CTACAAGGAG 
CGGCCGCAGG ACCTGGAGCA GCGTGAGTCG CCCCTCAACA ACTTCTCCGT GGCCCAATGC 
CAGCTGATGA AGACGGAGCG GCCGCGGCCC AACACCTTCA TCATCCGCTG CCTGCAGTGG 
ACCACGGTCA TCGAGCGCAC GTTCCACGTG GAGACGCCCG AGGAGCGGGA GGAGTGGACC 
ACCGCCATCC AGACGGTGGC CGACGGGCTC AAGAGGCAGG AGGAGGAGAC GATGGACTTC 
CGGTCGGGCT CACCCGGCGA GAACTCGGGG GCCGAGGAGA TGGAGGTGTC GCTGGCCAAG 
CCCAAGCACC GCGTGACCAT GAATGAGTTT GAGTACGTGA AGCTGCTGGG CAAAGGCACT 
TTCGGGAAGG TGATCCTGGT GAAGGAGAAG GCCACAGCGG CCTACTACGC CATGAAGATC 
CTAAAGAAGG AGGTCATCGT GGCCAAGGAC GAGGTGGCCC ACACGCTCAC AGAGAACCGC 
GTTCTCCAGA ACTCCCGGCA CCCGTCCCTG ACGGCCCTGA AGTACTCCTT CCAGACACAC 
GACCGCCTGT GCTTCGTCAT GGAGTACGCT AACGGGGGCG AGCTTTTCTT CCACCTGTCC 
CGGGAGCGGG TGTTTTCCGA GGACCGGGCC CGTTTCTACG GCGCCGAGAT CGTGTCGGCC 
CTGGATTACC TGCACTCGGA AAAGGAAGTG GTGTACAGGG ACCTCAAGCT GGAGAACCTC 
ATGCTGGACA AGGACGGGCA CATCAAGATC ACCGACTTCG GACTGTGCAA GGAGGGCATC 
AAGGATGGCG CCACCATGAA GACTTTCTGC GGGACCCCCG AATACCTGGC TCCCGAGGTG 
CTGGAGGACA ACGACTACGG CCGGGCAGTG GACTGGTGGG GGCTGGGTGT GGTCATGTAC 
GAGATGATGT GCGGCCGCCT GCCCTTCTAC AACCAGGACC ACGAGAAGCT CTTTGAGCTC 
ATTCTCATGG AGGAGATCCG CTTCCCCCGC ACACTCAGCC CGGAGGCCAA GTCCCTGCTC 
TCTGGGCTAC TCAAGAAGGA CCCCAAGCAG CGGCTTGGTG GGGGCTCTGA GGACGCCAAG 
GAGATCATGC AGCACCGATT CTTCGCCAGC ATCGTGTGGC AGGACGTGTA CGAGAAGAAG 
CTCAGCCCGC CCTTCAAGCC TCAGGTCACA TCTGAGACGG ACACCAGGTA TTTTGATGAG 
GAGTTCACGG CCCAGATGAT CACCATTACG CCACCTGACC AAGACGACAG CATGGAGGGG 
GTGGACAGCG AACGGAGGCC CCACTTCCCC CAGTTCTCCT ACTCGGCCAG CGCGACGGCC 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_776411.1
CDS82..1524
Translation

Target ORF information:

RefSeq Version NM_173986.2
Organism Bos taurus(cattle)
Definition Bos taurus AKT serine/threonine kinase 1 (AKT1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_173986.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGAACGACG TGGCCATCGT GAAGGAGGGC TGGCTGCACA AGCGAGGTGA GTACATCAAG 
ACGTGGCGGC CGCGTTACTT CCTCCTGAAG AACGATGGCA CGTTCATTGG CTACAAGGAG 
CGGCCGCAGG ACCTGGAGCA GCGTGAGTCG CCCCTCAACA ACTTCTCCGT GGCCCAATGC 
CAGCTGATGA AGACGGAGCG GCCGCGGCCC AACACCTTCA TCATCCGCTG CCTGCAGTGG 
ACCACGGTCA TCGAGCGCAC GTTCCACGTG GAGACGCCCG AGGAGCGGGA GGAGTGGACC 
ACCGCCATCC AGACGGTGGC CGACGGGCTC AAGAGGCAGG AGGAGGAGAC GATGGACTTC 
CGGTCGGGCT CACCCGGCGA GAACTCGGGG GCCGAGGAGA TGGAGGTGTC GCTGGCCAAG 
CCCAAGCACC GCGTGACCAT GAATGAGTTT GAGTACGTGA AGCTGCTGGG CAAAGGCACT 
TTCGGGAAGG TGATCCTGGT GAAGGAGAAG GCCACAGCGG CCTACTACGC CATGAAGATC 
CTAAAGAAGG AGGTCATCGT GGCCAAGGAC GAGGTGGCCC ACACGCTCAC AGAGAACCGC 
GTTCTCCAGA ACTCCCGGCA CCCGTCCCTG ACGGCCCTGA AGTACTCCTT CCAGACACAC 
GACCGCCTGT GCTTCGTCAT GGAGTACGCT AACGGGGGCG AGCTTTTCTT CCACCTGTCC 
CGGGAGCGGG TGTTTTCCGA GGACCGGGCC CGTTTCTACG GCGCCGAGAT CGTGTCGGCC 
CTGGATTACC TGCACTCGGA AAAGGAAGTG GTGTACAGGG ACCTCAAGCT GGAGAACCTC 
ATGCTGGACA AGGACGGGCA CATCAAGATC ACCGACTTCG GACTGTGCAA GGAGGGCATC 
AAGGATGGCG CCACCATGAA GACTTTCTGC GGGACCCCCG AATACCTGGC TCCCGAGGTG 
CTGGAGGACA ACGACTACGG CCGGGCAGTG GACTGGTGGG GGCTGGGTGT GGTCATGTAC 
GAGATGATGT GCGGCCGCCT GCCCTTCTAC AACCAGGACC ACGAGAAGCT CTTTGAGCTC 
ATTCTCATGG AGGAGATCCG CTTCCCCCGC ACACTCAGCC CGGAGGCCAA GTCCCTGCTC 
TCTGGGCTAC TCAAGAAGGA CCCCAAGCAG CGGCTTGGTG GGGGCTCTGA GGACGCCAAG 
GAGATCATGC AGCACCGATT CTTCGCCAGC ATCGTGTGGC AGGACGTGTA CGAGAAGAAG 
CTCAGCCCGC CCTTCAAGCC TCAGGTCACA TCTGAGACGG ACACCAGGTA TTTTGATGAG 
GAGTTCACGG CCCAGATGAT CACCATTACG CCACCTGACC AAGACGACAG CATGGAGGGG 
GTGGACAGCG AACGGAGGCC CCACTTCCCC CAGTTCTCCT ACTCGGCCAG CGCGACGGCC 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa228205
Clone ID Related Accession (Same CDS sequence) XM_024981592.1
Accession Version XM_024981592.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1443bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-06
Organism Bos taurus(cattle)
Product RAC-alpha serine/threonine-protein kinase isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGAACGACG TGGCCATCGT GAAGGAGGGC TGGCTGCACA AGCGAGGTGA GTACATCAAG 
ACGTGGCGGC CGCGTTACTT CCTCCTGAAG AACGATGGCA CGTTCATTGG CTACAAGGAG 
CGGCCGCAGG ACCTGGAGCA GCGTGAGTCG CCCCTCAACA ACTTCTCCGT GGCCCAATGC 
CAGCTGATGA AGACGGAGCG GCCGCGGCCC AACACCTTCA TCATCCGCTG CCTGCAGTGG 
ACCACGGTCA TCGAGCGCAC GTTCCACGTG GAGACGCCCG AGGAGCGGGA GGAGTGGACC 
ACCGCCATCC AGACGGTGGC CGACGGGCTC AAGAGGCAGG AGGAGGAGAC GATGGACTTC 
CGGTCGGGCT CACCCGGCGA GAACTCGGGG GCCGAGGAGA TGGAGGTGTC GCTGGCCAAG 
CCCAAGCACC GCGTGACCAT GAATGAGTTT GAGTACCTGA AGCTGCTGGG CAAAGGCACC 
TTCGGGAAGG TGATCCTGGT GAAGGAGAAG GCCACAGGCC GCTACTACGC CATGAAGATC 
CTAAAGAAGG AGGTCATCGT GGCCAAGGAC GAGGTGGCCC ACACGCTCAC AGAGAACCGC 
GTTCTCCAGA ACTCCCGGCA CCCGTTCCTG ACGGCCCTGA AGTACTCCTT CCAGACACAC 
GACCGCCTGT GCTTCGTCAT GGAGTACGCT AACGGGGGCG AGCTTTTCTT CCACCTGTCC 
CGGGAGCGGG TGTTTCCCGA GGACCGGGCC CGCTTCTACG GCGCCGAGAT CGTGTCGGCC 
CTGGATTACC TGCACTCGGA GAAGAACGTG GTGTACAGGG ACCTCAAGCT GGAGAACCTC 
ATGCTGGACA AGGACGGGCA CATCAAGATC ACCGACTTCG GACTGTGCAA GGAGGGCATC 
AAGGATGGCG CCACCATGAA GACTTTCTGC GGGACCCCCG AATACCTGGC TCCCGAGGTG 
CTGGAGGACA ACGACTACGG CCGGGCAGTG GACTGGTGGG GGCTGGGTGT GGTCATGTAC 
GAGATGATGT GCGGCCGCCT GCCCTTCTAC AACCAGGACC ACGAGAAGCT CTTTGAGCTC 
ATTCTCATGG AGGAGATCCG CTTCCCCCGC ACACTCAGCC CGGAGGCCAA GTCCCTGCTC 
TCTGGGCTAC TCAAGAAGGA CCCCAAGCAG CGGCTTGGTG GGGGCTCTGA GGACGCCAAG 
GAGATCATGC AGCACCGATT CTTCGCCAGC ATCGTGTGGC AGGACGTGTA CGAGAAGAAG 
CTCAGCCCGC CCTTCAAGCC TCAGGTCACA TCTGAGACGG ACACCAGGTA TTTTGATGAG 
GAGTTCACGG CCCAGATGAT CACCATTACG CCACCTGACC AAGACGACAG CATGGAGGGG 
GTGGACAGCG AACGGAGGCC CCACTTCCCC CAGTTCTCCT ACTCGGCCAG CGGCACGGCC 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_024837360.1
CDS210..1652
Translation

Target ORF information:

RefSeq Version XM_024981592.1
Organism Bos taurus(cattle)
Definition Bos taurus AKT serine/threonine kinase 1 (AKT1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_024981592.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGAACGACG TGGCCATCGT GAAGGAGGGC TGGCTGCACA AGCGAGGTGA GTACATCAAG 
ACGTGGCGGC CGCGTTACTT CCTCCTGAAG AACGATGGCA CGTTCATTGG CTACAAGGAG 
CGGCCGCAGG ACCTGGAGCA GCGTGAGTCG CCCCTCAACA ACTTCTCCGT GGCCCAATGC 
CAGCTGATGA AGACGGAGCG GCCGCGGCCC AACACCTTCA TCATCCGCTG CCTGCAGTGG 
ACCACGGTCA TCGAGCGCAC GTTCCACGTG GAGACGCCCG AGGAGCGGGA GGAGTGGACC 
ACCGCCATCC AGACGGTGGC CGACGGGCTC AAGAGGCAGG AGGAGGAGAC GATGGACTTC 
CGGTCGGGCT CACCCGGCGA GAACTCGGGG GCCGAGGAGA TGGAGGTGTC GCTGGCCAAG 
CCCAAGCACC GCGTGACCAT GAATGAGTTT GAGTACCTGA AGCTGCTGGG CAAAGGCACC 
TTCGGGAAGG TGATCCTGGT GAAGGAGAAG GCCACAGGCC GCTACTACGC CATGAAGATC 
CTAAAGAAGG AGGTCATCGT GGCCAAGGAC GAGGTGGCCC ACACGCTCAC AGAGAACCGC 
GTTCTCCAGA ACTCCCGGCA CCCGTTCCTG ACGGCCCTGA AGTACTCCTT CCAGACACAC 
GACCGCCTGT GCTTCGTCAT GGAGTACGCT AACGGGGGCG AGCTTTTCTT CCACCTGTCC 
CGGGAGCGGG TGTTTCCCGA GGACCGGGCC CGCTTCTACG GCGCCGAGAT CGTGTCGGCC 
CTGGATTACC TGCACTCGGA GAAGAACGTG GTGTACAGGG ACCTCAAGCT GGAGAACCTC 
ATGCTGGACA AGGACGGGCA CATCAAGATC ACCGACTTCG GACTGTGCAA GGAGGGCATC 
AAGGATGGCG CCACCATGAA GACTTTCTGC GGGACCCCCG AATACCTGGC TCCCGAGGTG 
CTGGAGGACA ACGACTACGG CCGGGCAGTG GACTGGTGGG GGCTGGGTGT GGTCATGTAC 
GAGATGATGT GCGGCCGCCT GCCCTTCTAC AACCAGGACC ACGAGAAGCT CTTTGAGCTC 
ATTCTCATGG AGGAGATCCG CTTCCCCCGC ACACTCAGCC CGGAGGCCAA GTCCCTGCTC 
TCTGGGCTAC TCAAGAAGGA CCCCAAGCAG CGGCTTGGTG GGGGCTCTGA GGACGCCAAG 
GAGATCATGC AGCACCGATT CTTCGCCAGC ATCGTGTGGC AGGACGTGTA CGAGAAGAAG 
CTCAGCCCGC CCTTCAAGCC TCAGGTCACA TCTGAGACGG ACACCAGGTA TTTTGATGAG 
GAGTTCACGG CCCAGATGAT CACCATTACG CCACCTGACC AAGACGACAG CATGGAGGGG 
GTGGACAGCG AACGGAGGCC CCACTTCCCC CAGTTCTCCT ACTCGGCCAG CGGCACGGCC 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa228206
Clone ID Related Accession (Same CDS sequence) XM_024981593.1
Accession Version XM_024981593.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1158bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-06
Organism Bos taurus(cattle)
Product RAC-alpha serine/threonine-protein kinase isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGAAGACGG AGCGGCCGCG GCCCAACACC TTCATCATCC GCTGCCTGCA GTGGACCACG 
GTCATCGAGC GCACGTTCCA CGTGGAGACG CCCGAGGAGC GGGAGGAGTG GACCACCGCC 
ATCCAGACGG TGGCCGACGG GCTCAAGAGG CAGGAGGAGG AGACGATGGA CTTCCGGTCG 
GGCTCACCCG GCGAGAACTC GGGGGCCGAG GAGATGGAGG TGTCGCTGGC CAAGCCCAAG 
CACCGCGTGA CCATGAATGA GTTTGAGTAC CTGAAGCTGC TGGGCAAAGG CACCTTCGGG 
AAGGTGATCC TGGTGAAGGA GAAGGCCACA GGCCGCTACT ACGCCATGAA GATCCTAAAG 
AAGGAGGTCA TCGTGGCCAA GGACGAGGTG GCCCACACGC TCACAGAGAA CCGCGTTCTC 
CAGAACTCCC GGCACCCGTT CCTGACGGCC CTGAAGTACT CCTTCCAGAC ACACGACCGC 
CTGTGCTTCG TCATGGAGTA CGCTAACGGG GGCGAGCTTT TCTTCCACCT GTCCCGGGAG 
CGGGTGTTTC CCGAGGACCG GGCCCGCTTC TACGGCGCCG AGATCGTGTC GGCCCTGGAT 
TACCTGCACT CGGAGAAGAA CGTGGTGTAC AGGGACCTCA AGCTGGAGAA CCTCATGCTG 
GACAAGGACG GGCACATCAA GATCACCGAC TTCGGACTGT GCAAGGAGGG CATCAAGGAT 
GGCGCCACCA TGAAGACTTT CTGCGGGACC CCCGAATACC TGGCTCCCGA GGACCACGAG 
AAGCTCTTTG AGCTCATTCT CATGGAGGAG ATCCGCTTCC CCCGCACACT CAGCCCGGAG 
GCCAAGTCCC TGCTCTCTGG GCTACTCAAG AAGGACCCCA AGCAGCGGCT TGGTGGGGGC 
TCTGAGGACG CCAAGGAGAT CATGCAGCAC CGATTCTTCG CCAGCATCGT GTGGCAGGAC 
GTGTACGAGA AGAAGCTCAG CCCGCCCTTC AAGCCTCAGG TCACATCTGA GACGGACACC 
AGGTATTTTG ATGAGGAGTT CACGGCCCAG ATGATCACCA TTACGCCACC TGACCAAGAC 
GACAGCATGG AGGGGGTGGA CAGCGAACGG AGGCCCCACT TCCCCCAGTT CTCCTACTCG 
GCCAGCGGCA CGGCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_024837361.1
CDS12..1169
Translation

Target ORF information:

RefSeq Version XM_024981593.1
Organism Bos taurus(cattle)
Definition Bos taurus AKT serine/threonine kinase 1 (AKT1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_024981593.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGAAGACGG AGCGGCCGCG GCCCAACACC TTCATCATCC GCTGCCTGCA GTGGACCACG 
GTCATCGAGC GCACGTTCCA CGTGGAGACG CCCGAGGAGC GGGAGGAGTG GACCACCGCC 
ATCCAGACGG TGGCCGACGG GCTCAAGAGG CAGGAGGAGG AGACGATGGA CTTCCGGTCG 
GGCTCACCCG GCGAGAACTC GGGGGCCGAG GAGATGGAGG TGTCGCTGGC CAAGCCCAAG 
CACCGCGTGA CCATGAATGA GTTTGAGTAC CTGAAGCTGC TGGGCAAAGG CACCTTCGGG 
AAGGTGATCC TGGTGAAGGA GAAGGCCACA GGCCGCTACT ACGCCATGAA GATCCTAAAG 
AAGGAGGTCA TCGTGGCCAA GGACGAGGTG GCCCACACGC TCACAGAGAA CCGCGTTCTC 
CAGAACTCCC GGCACCCGTT CCTGACGGCC CTGAAGTACT CCTTCCAGAC ACACGACCGC 
CTGTGCTTCG TCATGGAGTA CGCTAACGGG GGCGAGCTTT TCTTCCACCT GTCCCGGGAG 
CGGGTGTTTC CCGAGGACCG GGCCCGCTTC TACGGCGCCG AGATCGTGTC GGCCCTGGAT 
TACCTGCACT CGGAGAAGAA CGTGGTGTAC AGGGACCTCA AGCTGGAGAA CCTCATGCTG 
GACAAGGACG GGCACATCAA GATCACCGAC TTCGGACTGT GCAAGGAGGG CATCAAGGAT 
GGCGCCACCA TGAAGACTTT CTGCGGGACC CCCGAATACC TGGCTCCCGA GGACCACGAG 
AAGCTCTTTG AGCTCATTCT CATGGAGGAG ATCCGCTTCC CCCGCACACT CAGCCCGGAG 
GCCAAGTCCC TGCTCTCTGG GCTACTCAAG AAGGACCCCA AGCAGCGGCT TGGTGGGGGC 
TCTGAGGACG CCAAGGAGAT CATGCAGCAC CGATTCTTCG CCAGCATCGT GTGGCAGGAC 
GTGTACGAGA AGAAGCTCAG CCCGCCCTTC AAGCCTCAGG TCACATCTGA GACGGACACC 
AGGTATTTTG ATGAGGAGTT CACGGCCCAG ATGATCACCA TTACGCCACC TGACCAAGAC 
GACAGCATGG AGGGGGTGGA CAGCGAACGG AGGCCCCACT TCCCCCAGTT CTCCTACTCG 
GCCAGCGGCA CGGCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Prostaglandin F2?-induced luteolysis involves activation of Signal transducer and activator of transcription 3 and inhibition of AKT signaling in cattle.
Molecular reproduction and development84(6)486-494(2017 Jun)
Rovani MT,Ilha GF,Gasperin BG,N?brega JE Jr,Siddappa D,Glanzner WG,Antoniazzi AQ,Bordignon V,Duggavathi R,Gon?alves PBD


book

The role of the PI3K-Akt signaling pathway in the developmental competence of bovine oocytes.
PloS one12(9)e0185045(2017)
Andrade GM,da Silveira JC,Perrini C,Del Collado M,Gebremedhn S,Tesfaye D,Meirelles FV,Perecin F


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Protein kinase B is required for follicle-stimulating hormone mediated beta-catenin accumulation and estradiol production in granulosa cells of cattle.
Animal reproduction science16397-104(2015 Dec)
G?mez BI,Gifford CA,Hallford DM,Hernandez Gifford JA


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Caveolin-1 scaffolding domain residue phenylalanine 92 modulates Akt signaling.
European journal of pharmacology76646-55(2015 Nov)
Trane AE,Hiob MA,Uy T,Pavlov D,Bernatchez P