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VEGFA cDNA ORF clone, Bos taurus(Cattle)

Gene Symbol VEGFA
Entrez Gene ID 281572
Full Name vascular endothelial growth factor A
Synonyms VEGF,VEGF-A,VPF,eVEGF120,eVEGF164
Gene Type protein-coding
Organism Bos taurus(Cattle)
Summary This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. It is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. [provided by RefSeq, Nov 2015].

mRNA and Protein(s)

mRNA Protein Name
NM_001316993.1 NP_001303922.1 vascular endothelial growth factor A isoform 2 precursor
NM_001316956.1 NP_001303885.1 vascular endothelial growth factor A isoform 4 precursor
NM_001316955.1 NP_001303884.1 vascular endothelial growth factor A isoform VEGF-Ax precursor
NM_001316992.1 NP_001303921.1 vascular endothelial growth factor A isoform 3 precursor
NM_174216.2 NP_776641.1 vascular endothelial growth factor A isoform VEGF-A precursor



Rattus norvegicus (Norway rat) Vegfa NP_114024.2
Gallus gallus (chicken) VEGFA NP_990373.1
Canis lupus familiaris (dog) VEGFA NP_001003175.2
Bos taurus (cattle) VEGFA NP_776641.1
Mus musculus (house mouse) Vegfa NP_001020421.2
Homo sapiens (human) VEGFA NP_003367.4
Danio rerio (zebrafish) vegfab NP_001038320.2


Related articles in PubMed

The interplay of cyclic stretch and vascular endothelial growth factor in regulating the initial steps for angiogenesis.
Wilkins JR, Pike DB, Gibson CC, Li L, Shiu YT
Biotechnology progress31(1)248-57(2015 Jan-Feb)

Programmed translational readthrough generates antiangiogenic VEGF-Ax.
Eswarappa SM, Potdar AA, Koch WJ, Fan Y, Vasu K, Lindner D, Willard B, Graham LM, DiCorleto PE, Fox PL
Cell157(7)1605-18(2014 Jun)

Targeting the IRE1α/XBP1 and ATF6 arms of the unfolded protein response enhances VEGF blockade to prevent retinal and choroidal neovascularization.
Liu L, Qi X, Chen Z, Shaw L, Cai J, Smith LH, Grant MB, Boulton ME
The American journal of pathology182(4)1412-24(2013 Apr)

Diabetes-induced superoxide anion and breakdown of the blood-retinal barrier: role of the VEGF/uPAR pathway.
El-Remessy AB, Franklin T, Ghaley N, Yang J, Brands MW, Caldwell RB, Behzadian MA
PloS one8(8)e71868(2013)

Advanced glycation end products increase permeability of brain microvascular endothelial cells through reactive oxygen species-induced vascular endothelial growth factor expression.
Niiya Y, Abumiya T, Yamagishi S, Takino J, Takeuchi M
Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association21(4)293-8(2012 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

data for the first time demonstrate a calpain/PTP1B/VEGFR2 negative feedback loop in the regulation of VEGF-induced angiogenesis. Modulation of local PTP1B and/or calpain activities may prove beneficial in the treatment of impaired wound healing in diabetes.
Title: Protein Phosphotyrosine Phosphatase 1B (PTP1B) in Calpain-dependent Feedback Regulation of Vascular Endothelial Growth Factor Receptor (VEGFR2) in Endothelial Cells: IMPLICATIONS IN VEGF-DEPENDENT ANGIOGENESIS AND DIABETIC WOUND HEALING.

Studied the effect of cyclic uniaxial stretch (20%, 1 Hz), with and without the stimulation of vascular endothelial growth factor (VEGF), on sprouting angiogenesis by employing a stretchable three-dimensional cell culture model.
Title: The interplay of cyclic stretch and vascular endothelial growth factor in regulating the initial steps for angiogenesis.

Study shows that VEGFA mRNA in mammalian endothelial cells undergoes programmed translational readthrough (PTR) generating VEGF-Ax, an isoform containing a unique 22-amino-acid C terminus extension.
Title: Programmed translational readthrough generates antiangiogenic VEGF-Ax.

Exposure of endothelial cells to VEGF, high glucose, or hydrogen peroxide up-regulated the XBP1/IRE1 alpha and ATF6 arms of the unfolded protein response compared with untreated cells.
Title: Targeting the IRE1α/XBP1 and ATF6 arms of the unfolded protein response enhances VEGF blockade to prevent retinal and choroidal neovascularization.

Data indicate that superoxide dismutase (SOD) inhibited high glucose (HG)-induced expression of uPAR and VEGF in bovine retinal microvascular endothelial cell (REC).
Title: Diabetes-induced superoxide anion and breakdown of the blood-retinal barrier: role of the VEGF/uPAR pathway.

The following VEGFA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VEGFA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OBa01429 NM_174216.2
Latest version!
Bos taurus vascular endothelial growth factor A (VEGFA), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $104.30
$149.00
OBa45168 NM_001316993.1
Latest version!
Bos taurus vascular endothelial growth factor A (VEGFA), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $104.30
$149.00
OBa134049 NM_001316956.1
Latest version!
Bos taurus vascular endothelial growth factor A (VEGFA), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $174.30
$249.00
OBa134050 NM_001316992.1
Latest version!
Bos taurus vascular endothelial growth factor A (VEGFA), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $104.30
$149.00
OBa95409 NM_001316955.1
Latest version!
Bos taurus vascular endothelial growth factor A (VEGFA), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $174.30
$249.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OBa01429
Clone ID Related Accession (Same CDS sequence) NM_174216.2
Accession Version NM_174216.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 573bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-23
Organism Bos taurus(Cattle)
Product vascular endothelial growth factor A isoform VEGF-A precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EV647554.1, HX921266.1, M32976.1, DV876964.1 and DV814183.1. On Nov 4, 2015 this sequence version replaced NM_174216.1. Transcript Variant: This variant (1) represents the predominant transcript and encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (VEGF-A, also known as VEGF-A164) results from translation termination at the upstream UGA stop codon (nt 1618-1620), while the longer isoform (VEGF-Ax) results from UGA stop codon readthrough to the downstream UGA termination codon (nt 1684-1686). This RefSeq represents the shorter isoform, which exhibits proangiogenic activity. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.

1
61
121
181
241
301
361
421
481
541
ATGAACTTTC TGCTCTCTTG GGTACATTGG AGCCTTGCCT TGCTGCTCTA CCTTCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGGC AGAAACCCCA CGAAGTGGTG
AAGTTCATGG ATGTCTACCA GCGCAGCTTC TGCCGTCCCA TCGAGACCCT GGTGGACATC
TTCCAGGAGT ACCCAGATGA GATTGAGTTC ATTTTCAAGC CGTCCTGTGT GCCCCTGATG
CGGTGCGGGG GCTGCTGTAA TGACGAAAGT CTGGAGTGTG TGCCCACTGA GGAGTTCAAC
ATCACCATGC AGATTATGCG GATCAAACCT CACCAAAGCC AGCACATAGG AGAGATGAGC
TTCCTACAGC ATAACAAATG TGAATGCAGA CCAAAGAAAG ATAAAGCAAG GCAAGAAAAT
CCCTGTGGGC CTTGCTCAGA GCGGAGAAAG CATTTGTTTG TACAAGATCC GCAGACGTGT
AAATGTTCCT GCAAAAACAC AGACTCGCGT TGCAAGGCGA GGCAGCTTGA GTTAAACGAA
CGTACTTGCA GATGTGACAA GCCGAGGCGG TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_776641.1
CDS1048..1620
Misc Feature(1)427..429
Misc Feature(2)1192..1440
Misc Feature(3)order(1192..1203,1303..1305,1309..1320,1363..1374)
Misc Feature(4)order(1198..1200,1291..1293,1303..1305,1324..1326,1426..1428,1432..1434)
Misc Feature(5)order(1273..1275,1300..1302)
Misc Feature(6)1444..1617
Misc Feature(7)1684..1686
Translation

Target ORF information:

RefSeq Version NM_174216.2
Organism Bos taurus(Cattle)
Definition Bos taurus vascular endothelial growth factor A (VEGFA), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_174216.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGAACTTTC TGCTCTCTTG GGTACATTGG AGCCTTGCCT TGCTGCTCTA CCTTCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGGC AGAAACCCCA CGAAGTGGTG
AAGTTCATGG ATGTCTACCA GCGCAGCTTC TGCCGTCCCA TCGAGACCCT GGTGGACATC
TTCCAGGAGT ACCCAGATGA GATTGAGTTC ATTTTCAAGC CGTCCTGTGT GCCCCTGATG
CGGTGCGGGG GCTGCTGTAA TGACGAAAGT CTGGAGTGTG TGCCCACTGA GGAGTTCAAC
ATCACCATGC AGATTATGCG GATCAAACCT CACCAAAGCC AGCACATAGG AGAGATGAGC
TTCCTACAGC ATAACAAATG TGAATGCAGA CCAAAGAAAG ATAAAGCAAG GCAAGAAAAT
CCCTGTGGGC CTTGCTCAGA GCGGAGAAAG CATTTGTTTG TACAAGATCC GCAGACGTGT
AAATGTTCCT GCAAAAACAC AGACTCGCGT TGCAAGGCGA GGCAGCTTGA GTTAAACGAA
CGTACTTGCA GATGTGACAA GCCGAGGCGG TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa45168
Clone ID Related Accession (Same CDS sequence) NM_001316993.1
Accession Version NM_001316993.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 441bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-23
Organism Bos taurus(Cattle)
Product vascular endothelial growth factor A isoform 2 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EV647554.1, HX921266.1, M32976.1, AB455252.1, DV876964.1 and DV814183.1. Transcript Variant: This variant (2) lacks an in-frame coding exon compared to variant 1, and encodes a shorter isoform (2, also known as VEGF-A120) missing an internal protein segment compared to isoform VEGF-A. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.

1
61
121
181
241
301
361
421
ATGAACTTTC TGCTCTCTTG GGTACATTGG AGCCTTGCCT TGCTGCTCTA CCTTCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGGC AGAAACCCCA CGAAGTGGTG
AAGTTCATGG ATGTCTACCA GCGCAGCTTC TGCCGTCCCA TCGAGACCCT GGTGGACATC
TTCCAGGAGT ACCCAGATGA GATTGAGTTC ATTTTCAAGC CGTCCTGTGT GCCCCTGATG
CGGTGCGGGG GCTGCTGTAA TGACGAAAGT CTGGAGTGTG TGCCCACTGA GGAGTTCAAC
ATCACCATGC AGATTATGCG GATCAAACCT CACCAAAGCC AGCACATAGG AGAGATGAGC
TTCCTACAGC ATAACAAATG TGAATGCAGA CCAAAGAAAG ATAAAGCAAG GCAAGAAAAA
TGTGACAAGC CGAGGCGGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001303922.1
CDS1048..1488
Misc Feature(1)427..429
Misc Feature(2)1192..1440
Misc Feature(3)order(1192..1203,1303..1305,1309..1320,1363..1374)
Misc Feature(4)order(1198..1200,1291..1293,1303..1305,1324..1326,1426..1428,1432..1434)
Misc Feature(5)order(1273..1275,1300..1302)
Translation

Target ORF information:

RefSeq Version NM_001316993.1
Organism Bos taurus(Cattle)
Definition Bos taurus vascular endothelial growth factor A (VEGFA), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001316993.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
ATGAACTTTC TGCTCTCTTG GGTACATTGG AGCCTTGCCT TGCTGCTCTA CCTTCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGGC AGAAACCCCA CGAAGTGGTG
AAGTTCATGG ATGTCTACCA GCGCAGCTTC TGCCGTCCCA TCGAGACCCT GGTGGACATC
TTCCAGGAGT ACCCAGATGA GATTGAGTTC ATTTTCAAGC CGTCCTGTGT GCCCCTGATG
CGGTGCGGGG GCTGCTGTAA TGACGAAAGT CTGGAGTGTG TGCCCACTGA GGAGTTCAAC
ATCACCATGC AGATTATGCG GATCAAACCT CACCAAAGCC AGCACATAGG AGAGATGAGC
TTCCTACAGC ATAACAAATG TGAATGCAGA CCAAAGAAAG ATAAAGCAAG GCAAGAAAAA
TGTGACAAGC CGAGGCGGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa134049
Clone ID Related Accession (Same CDS sequence) NM_001316956.1
Accession Version NM_001316956.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 645bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-23
Organism Bos taurus(Cattle)
Product vascular endothelial growth factor A isoform 4 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EV647554.1, HX921266.1, M32976.1, AB450824.1, DV876964.1 and DV814183.1. Transcript Variant: This variant (4) contains an additional in-frame coding exon compared to variant 1, and encodes a longer isoform (4) with an internal 24 aa protein segment missing in isoform VEGF-A. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.

1
61
121
181
241
301
361
421
481
541
601
ATGAACTTTC TGCTCTCTTG GGTACATTGG AGCCTTGCCT TGCTGCTCTA CCTTCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGGC AGAAACCCCA CGAAGTGGTG
AAGTTCATGG ATGTCTACCA GCGCAGCTTC TGCCGTCCCA TCGAGACCCT GGTGGACATC
TTCCAGGAGT ACCCAGATGA GATTGAGTTC ATTTTCAAGC CGTCCTGTGT GCCCCTGATG
CGGTGCGGGG GCTGCTGTAA TGACGAAAGT CTGGAGTGTG TGCCCACTGA GGAGTTCAAC
ATCACCATGC AGATTATGCG GATCAAACCT CACCAAAGCC AGCACATAGG AGAGATGAGC
TTCCTACAGC ATAACAAATG TGAATGCAGA CCAAAGAAAG ATAAAGCAAG GCAAGAAAAA
AAATCAGTTC GAGGAAAGGG CAAAGGGCAA AAAAGAAAGC GCAAGAAATC CCGGTATAAA
TCCTGGAGCG CTCCCTGTGG GCCTTGCTCA GAGCGGAGAA AGCATTTGTT TGTACAAGAT
CCGCAGACGT GTAAATGTTC CTGCAAAAAC ACAGACTCGC GTTGCAAGGC GAGGCAGCTT
GAGTTAAACG AACGTACTTG CAGATGTGAC AAGCCGAGGC GGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001303885.1
CDS1048..1692
Misc Feature(1)427..429
Misc Feature(2)1192..1440
Misc Feature(3)order(1192..1203,1303..1305,1309..1320,1363..1374)
Misc Feature(4)order(1198..1200,1291..1293,1303..1305,1324..1326,1426..1428,1432..1434)
Misc Feature(5)order(1273..1275,1300..1302)
Misc Feature(6)1543..1689
Translation

Target ORF information:

RefSeq Version NM_001316956.1
Organism Bos taurus(Cattle)
Definition Bos taurus vascular endothelial growth factor A (VEGFA), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001316956.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGAACTTTC TGCTCTCTTG GGTACATTGG AGCCTTGCCT TGCTGCTCTA CCTTCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGGC AGAAACCCCA CGAAGTGGTG
AAGTTCATGG ATGTCTACCA GCGCAGCTTC TGCCGTCCCA TCGAGACCCT GGTGGACATC
TTCCAGGAGT ACCCAGATGA GATTGAGTTC ATTTTCAAGC CGTCCTGTGT GCCCCTGATG
CGGTGCGGGG GCTGCTGTAA TGACGAAAGT CTGGAGTGTG TGCCCACTGA GGAGTTCAAC
ATCACCATGC AGATTATGCG GATCAAACCT CACCAAAGCC AGCACATAGG AGAGATGAGC
TTCCTACAGC ATAACAAATG TGAATGCAGA CCAAAGAAAG ATAAAGCAAG GCAAGAAAAA
AAATCAGTTC GAGGAAAGGG CAAAGGGCAA AAAAGAAAGC GCAAGAAATC CCGGTATAAA
TCCTGGAGCG CTCCCTGTGG GCCTTGCTCA GAGCGGAGAA AGCATTTGTT TGTACAAGAT
CCGCAGACGT GTAAATGTTC CTGCAAAAAC ACAGACTCGC GTTGCAAGGC GAGGCAGCTT
GAGTTAAACG AACGTACTTG CAGATGTGAC AAGCCGAGGC GGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa134050
Clone ID Related Accession (Same CDS sequence) NM_001316992.1
Accession Version NM_001316992.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 573bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-23
Organism Bos taurus(Cattle)
Product vascular endothelial growth factor A isoform 3 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EV647554.1, HX921266.1, M32976.1, EU017524.1, DV876964.1 and DV814183.1. Transcript Variant: This variant (3) uses an alternate acceptor splice site at the 3' terminal exon, which causes a frameshift compared to variant 1. The encoded isoform (3, also known as VEGF-Ab) has a distinct C-terminus compared to isoform VEGF-A. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.

1
61
121
181
241
301
361
421
481
541
ATGAACTTTC TGCTCTCTTG GGTACATTGG AGCCTTGCCT TGCTGCTCTA CCTTCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGGC AGAAACCCCA CGAAGTGGTG
AAGTTCATGG ATGTCTACCA GCGCAGCTTC TGCCGTCCCA TCGAGACCCT GGTGGACATC
TTCCAGGAGT ACCCAGATGA GATTGAGTTC ATTTTCAAGC CGTCCTGTGT GCCCCTGATG
CGGTGCGGGG GCTGCTGTAA TGACGAAAGT CTGGAGTGTG TGCCCACTGA GGAGTTCAAC
ATCACCATGC AGATTATGCG GATCAAACCT CACCAAAGCC AGCACATAGG AGAGATGAGC
TTCCTACAGC ATAACAAATG TGAATGCAGA CCAAAGAAAG ATAAAGCAAG GCAAGAAAAT
CCCTGTGGGC CTTGCTCAGA GCGGAGAAAG CATTTGTTTG TACAAGATCC GCAGACGTGT
AAATGTTCCT GCAAAAACAC AGACTCGCGT TGCAAGGCGA GGCAGCTTGA GTTAAACGAA
CGTACTTGCA GACGTCTCAC CAGGAAAGAC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001303921.1
CDS1048..1620
Misc Feature(1)427..429
Misc Feature(2)1192..1440
Misc Feature(3)order(1192..1203,1303..1305,1309..1320,1363..1374)
Misc Feature(4)order(1198..1200,1291..1293,1303..1305,1324..1326,1426..1428,1432..1434)
Misc Feature(5)order(1273..1275,1300..1302)
Misc Feature(6)1444..1599
Translation

Target ORF information:

RefSeq Version NM_001316992.1
Organism Bos taurus(Cattle)
Definition Bos taurus vascular endothelial growth factor A (VEGFA), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001316992.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGAACTTTC TGCTCTCTTG GGTACATTGG AGCCTTGCCT TGCTGCTCTA CCTTCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGGC AGAAACCCCA CGAAGTGGTG
AAGTTCATGG ATGTCTACCA GCGCAGCTTC TGCCGTCCCA TCGAGACCCT GGTGGACATC
TTCCAGGAGT ACCCAGATGA GATTGAGTTC ATTTTCAAGC CGTCCTGTGT GCCCCTGATG
CGGTGCGGGG GCTGCTGTAA TGACGAAAGT CTGGAGTGTG TGCCCACTGA GGAGTTCAAC
ATCACCATGC AGATTATGCG GATCAAACCT CACCAAAGCC AGCACATAGG AGAGATGAGC
TTCCTACAGC ATAACAAATG TGAATGCAGA CCAAAGAAAG ATAAAGCAAG GCAAGAAAAT
CCCTGTGGGC CTTGCTCAGA GCGGAGAAAG CATTTGTTTG TACAAGATCC GCAGACGTGT
AAATGTTCCT GCAAAAACAC AGACTCGCGT TGCAAGGCGA GGCAGCTTGA GTTAAACGAA
CGTACTTGCA GACGTCTCAC CAGGAAAGAC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa95409
Clone ID Related Accession (Same CDS sequence) NM_001316955.1
Accession Version NM_001316955.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 639bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-02-24
Organism Bos taurus(Cattle)
Product vascular endothelial growth factor A isoform VEGF-Ax precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EV647554.1, HX921266.1, M32976.1, DV876964.1 and DV814183.1. Transcript Variant: This variant (1) represents the predominant transcript and encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (VEGF-A, also known as VEGF-A164) results from translation termination at the upstream UGA stop codon (nt 1618-1620), while the longer isoform (VEGF-Ax) results from UGA stop codon readthrough to the downstream UGA termination codon (nt 1684-1686). This RefSeq represents the longer, C-terminally extended isoform, which exhibits antiangiogenic activity. As the upstream UGA stop codon has been shown to be recoded as serine (PMID:24949972), its location in the VEGF-Ax isoform (aa 191) is denoted by an 'S'. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## stop codon readthrough :: PMID: 24949972 ##RefSeq-Attributes-END##

1
61
121
181
241
301
361
421
481
541
601
ATGAACTTTC TGCTCTCTTG GGTACATTGG AGCCTTGCCT TGCTGCTCTA CCTTCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGGC AGAAACCCCA CGAAGTGGTG
AAGTTCATGG ATGTCTACCA GCGCAGCTTC TGCCGTCCCA TCGAGACCCT GGTGGACATC
TTCCAGGAGT ACCCAGATGA GATTGAGTTC ATTTTCAAGC CGTCCTGTGT GCCCCTGATG
CGGTGCGGGG GCTGCTGTAA TGACGAAAGT CTGGAGTGTG TGCCCACTGA GGAGTTCAAC
ATCACCATGC AGATTATGCG GATCAAACCT CACCAAAGCC AGCACATAGG AGAGATGAGC
TTCCTACAGC ATAACAAATG TGAATGCAGA CCAAAGAAAG ATAAAGCAAG GCAAGAAAAT
CCCTGTGGGC CTTGCTCAGA GCGGAGAAAG CATTTGTTTG TACAAGATCC GCAGACGTGT
AAATGTTCCT GCAAAAACAC AGACTCGCGT TGCAAGGCGA GGCAGCTTGA GTTAAACGAA
CGTACTTGCA GATGTGACAA GCCGAGGCGG TGAGCCGGGC TGGAGGAAGG AGCCTCCCTC
AGGGTTTCGG GAACCAGACG TCTCACCAGG AAAGACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001303884.1
CDS1048..1686
Misc Feature(1)427..429
Misc Feature(2)1192..1440
Misc Feature(3)order(1192..1203,1303..1305,1309..1320,1363..1374)
Misc Feature(4)order(1198..1200,1291..1293,1303..1305,1324..1326,1426..1428,1432..1434)
Misc Feature(5)order(1273..1275,1300..1302)
Misc Feature(6)1444..1617
Misc Feature(7)1618..1620
Misc Feature(8)1621..1683
Misc Feature(9)1631..1641
Translation

Target ORF information:

RefSeq Version NM_001316955.1
Organism Bos taurus(Cattle)
Definition Bos taurus vascular endothelial growth factor A (VEGFA), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001316955.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGAACTTTC TGCTCTCTTG GGTACATTGG AGCCTTGCCT TGCTGCTCTA CCTTCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGGC AGAAACCCCA CGAAGTGGTG
AAGTTCATGG ATGTCTACCA GCGCAGCTTC TGCCGTCCCA TCGAGACCCT GGTGGACATC
TTCCAGGAGT ACCCAGATGA GATTGAGTTC ATTTTCAAGC CGTCCTGTGT GCCCCTGATG
CGGTGCGGGG GCTGCTGTAA TGACGAAAGT CTGGAGTGTG TGCCCACTGA GGAGTTCAAC
ATCACCATGC AGATTATGCG GATCAAACCT CACCAAAGCC AGCACATAGG AGAGATGAGC
TTCCTACAGC ATAACAAATG TGAATGCAGA CCAAAGAAAG ATAAAGCAAG GCAAGAAAAT
CCCTGTGGGC CTTGCTCAGA GCGGAGAAAG CATTTGTTTG TACAAGATCC GCAGACGTGT
AAATGTTCCT GCAAAAACAC AGACTCGCGT TGCAAGGCGA GGCAGCTTGA GTTAAACGAA
CGTACTTGCA GATGTGACAA GCCGAGGCGG TGAGCCGGGC TGGAGGAAGG AGCCTCCCTC
AGGGTTTCGG GAACCAGACG TCTCACCAGG AAAGACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

The interplay of cyclic stretch and vascular endothelial growth factor in regulating the initial steps for angiogenesis.
Biotechnology progress31(1)248-57(2015 Jan-Feb)
Wilkins JR,Pike DB,Gibson CC,Li L,Shiu YT


book

Programmed translational readthrough generates antiangiogenic VEGF-Ax.
Cell157(7)1605-18(2014 Jun)
Eswarappa SM,Potdar AA,Koch WJ,Fan Y,Vasu K,Lindner D,Willard B,Graham LM,DiCorleto PE,Fox PL


book

Targeting the IRE1α/XBP1 and ATF6 arms of the unfolded protein response enhances VEGF blockade to prevent retinal and choroidal neovascularization.
The American journal of pathology182(4)1412-24(2013 Apr)
Liu L,Qi X,Chen Z,Shaw L,Cai J,Smith LH,Grant MB,Boulton ME


book

Diabetes-induced superoxide anion and breakdown of the blood-retinal barrier: role of the VEGF/uPAR pathway.
PloS one8(8)e71868(2013)
El-Remessy AB,Franklin T,Ghaley N,Yang J,Brands MW,Caldwell RB,Behzadian MA


book

Advanced glycation end products increase permeability of brain microvascular endothelial cells through reactive oxygen species-induced vascular endothelial growth factor expression.
Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association21(4)293-8(2012 May)
Niiya Y,Abumiya T,Yamagishi S,Takino J,Takeuchi M


 
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