VEGFA cDNA ORF clone, Homo sapiens(human)

  • Gene

  • Clones

  • gRNAs

The following VEGFA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VEGFA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu26665 NM_001171629.1
Latest version!
Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 7, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$49.50-$69.30
$99.00
OHu26685 NM_001025366.3
Latest version!
Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$174.50-$244.30
$349.00
OHu26721 NM_001025367.3
Latest version!
Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$164.50-$230.30
$329.00
OHu26819 NM_001171623.1
Latest version!
Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$99.50-$139.30
$199.00
OHu26825 NM_001171628.1
Latest version!
Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$49.50-$69.30
$99.00
OHu26865 NM_001171627.1
Latest version!
Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$49.50-$69.30
$99.00
OHu26938 NM_001171625.1
Latest version!
Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$134.50-$188.30
$269.00
OHu26944 NM_001171624.1
Latest version!
Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$154.50-$216.30
$309.00
OHu27131 NM_001025368.3
Latest version!
Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$49.50-$69.30
$99.00
OHu27146 NM_001171626.1
Latest version!
Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$49.50-$69.30
$99.00
OHu27630 NM_001287044.2
Latest version!
Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 10, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$49.50-$69.30
$99.00
OHu14341 NM_001204384.1
Latest version!
Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $69.30
$99.00
OHu26728 NM_001171630.1
Latest version!
Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $69.30
$99.00
OHu102720 NM_001317010.1
Latest version!
Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $139.30
$199.00
OHu14217 NM_001204385.2
Latest version!
Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $223.30
$319.00
OHu26588 NM_001025369.3
Latest version!
Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $223.30
$319.00
OHu26828 NM_001025370.3
Latest version!
Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $139.30
$199.00
OHu26860 NM_003376.6
Latest version!
Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $223.30
$319.00
OHu27292 NM_001033756.3
Latest version!
Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $223.30
$319.00
OHu27323 NM_001171622.2
Latest version!
Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $139.30
$199.00
View Old Accession Versions >>
OHu26828 NM_001025370.2 Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $139.30
$199.00
OHu26721 NM_001025367.2 Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$164.50-$230.30
$329.00
OHu26860 NM_003376.5 Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $223.30
$319.00
OHu27630 NM_001287044.1 Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 10, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$49.50-$69.30
$99.00
OHu14217 NM_001204385.1 Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $223.30
$319.00
OHu26588 NM_001025369.2 Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $223.30
$319.00
OHu27131 NM_001025368.2 Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$49.50-$69.30
$99.00
OHu26685 NM_001025366.2 Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$174.50-$244.30
$349.00
OHu27292 NM_001033756.2 Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $223.30
$319.00
OHu27323 NM_001171622.1 Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $139.30
$199.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu26665
    Clone ID Related Accession (Same CDS sequence) NM_001171629.1
    Accession Version NM_001171629.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 576bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-21
    Organism Homo sapiens(human)
    Product vascular endothelial growth factor A isoform o precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA237741.1, CN256173.1, BU153227.1, AF430806.1, AF024710.1, BF700556.1 and BM661679.1. Transcript Variant: This variant (7) lacks an alternate in-frame exon and uses an alternate splice site that causes a frameshift in the 3' coding region, compared to variant 1. This variant can initiate translation from four non-AUG (CUG) sites, and also from a downstream, in-frame AUG. The isoform (o, also referred to as VEGF165b) represented in this RefSeq is derived from the AUG start codon, and has a distinct C-terminus and is shorter than isoform a. CCDS Note: This CCDS, which is supported by the mRNA AF430806.1, represents a VEGFA isoform derived from an AUG start codon. Alternative translation initiation from multiple upstream non-AUG(CUG) codons is also possible for this gene, as indicated in PMIDs:11352659, 11563986 and 11731620. The longest isoform encoded by this variant, which is derived from the 5'-most CUG start codon, is represented by CCDS 47432.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF430806.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## regulatory uORF :: PMID: 18304943 ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
    GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG
    GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC
    ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG
    ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC
    AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG
    AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA
    AATCCCTGTG GGCCTTGCTC AGAGCGGAGA AAGCATTTGT TTGTACAAGA TCCGCAGACG
    TGTAAATGTT CCTGCAAAAA CACAGACTCG CGTTGCAAGG CGAGGCAGCT TGAGTTAAAC
    GAACGTACTT GCAGATCTCT CACCAGGAAA GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001165100.1
    CDS1039..1614
    Translation

    Target ORF information:

    RefSeq Version NM_001171629.1
    Organism Homo sapiens(human)
    Definition Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001171629.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
    GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG
    GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC
    ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG
    ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC
    AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG
    AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA
    AATCCCTGTG GGCCTTGCTC AGAGCGGAGA AAGCATTTGT TTGTACAAGA TCCGCAGACG
    TGTAAATGTT CCTGCAAAAA CACAGACTCG CGTTGCAAGG CGAGGCAGCT TGAGTTAAAC
    GAACGTACTT GCAGATCTCT CACCAGGAAA GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu26685
    Clone ID Related Accession (Same CDS sequence) NM_001025366.3 , NM_001025366.2
    Accession Version NM_001025366.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1239bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-07
    Organism Homo sapiens(human)
    Product vascular endothelial growth factor A isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA237741.1, CN256173.1, BU153227.1, S85192.1, AF024710.1, BF700556.1 and BM661679.1. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_001025366.2. Transcript Variant: This variant (1) represents the longest transcript. This variant can initiate translation from four non-AUG (CUG) sites, and also from a downstream, in-frame AUG. The isoform (a) represented in this RefSeq is derived from the 5'-most CUG start codon. This is the longest isoform. CCDS Note: This CCDS representation starts at an upstream non-AUG (CUG) translation start codon that is supported by conservation data and experimental evidence, including that in PMIDs:11352659, 11563986 and 11731620. Most of the publicly available long mRNAs at this locus do not extend far enough 5' to include the CUG start codon, including S85192.1 which supports this variant. However, BC058855.1, several unspliced partial transcripts and the aforementioned publications all support transcription initiation further upstream. Hence, this CCDS representation includes the extended 5' CDS. Alternative translation initiation from a downstream AUG codon (Met-181), which results in a shorter isoform with a predicted signal peptide, has also been shown for this gene. The shorter isoform encoded by this variant is represented by CCDS 55010.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: S85192.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2145240 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 11352659, 11563986, 11731620 ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
    CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC
    GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC
    TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG
    AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG
    AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG
    GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC
    CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC
    CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC
    ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT
    GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG
    GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC
    ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG
    ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC
    AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG
    AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA
    AAAAAATCAG TTCGAGGAAA GGGAAAGGGG CAAAAACGAA AGCGCAAGAA ATCCCGGTAT
    AAGTCCTGGA GCGTGTACGT TGGTGCCCGC TGCTGTCTAA TGCCCTGGAG CCTCCCTGGC
    CCCCATCCCT GTGGGCCTTG CTCAGAGCGG AGAAAGCATT TGTTTGTACA AGATCCGCAG
    ACGTGTAAAT GTTCCTGCAA AAACACAGAC TCGCGTTGCA AGGCGAGGCA GCTTGAGTTA
    AACGAACGTA CTTGCAGATG TGACAAGCCG AGGCGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001020537.2
    CDS497..1735
    Translation

    Target ORF information:

    RefSeq Version NM_001025366.3
    Organism Homo sapiens(human)
    Definition Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001025366.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
    CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC
    GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC
    TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG
    AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG
    AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG
    GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC
    CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC
    CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC
    ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT
    GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG
    GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC
    ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG
    ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC
    AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG
    AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA
    AAAAAATCAG TTCGAGGAAA GGGAAAGGGG CAAAAACGAA AGCGCAAGAA ATCCCGGTAT
    AAGTCCTGGA GCGTGTACGT TGGTGCCCGC TGCTGTCTAA TGCCCTGGAG CCTCCCTGGC
    CCCCATCCCT GTGGGCCTTG CTCAGAGCGG AGAAAGCATT TGTTTGTACA AGATCCGCAG
    ACGTGTAAAT GTTCCTGCAA AAACACAGAC TCGCGTTGCA AGGCGAGGCA GCTTGAGTTA
    AACGAACGTA CTTGCAGATG TGACAAGCCG AGGCGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu26685
    Clone ID Related Accession (Same CDS sequence) NM_001025366.3 , NM_001025366.2
    Accession Version NM_001025366.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1239bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-03
    Organism Homo sapiens(human)
    Product vascular endothelial growth factor A isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA237741.1, CN256173.1, BU153227.1, S85192.1, AF024710.1, BF700556.1 and BM661679.1. This sequence is a reference standard in the RefSeqGene project. On Jan 21, 2010 this sequence version replaced NM_001025366.1. Transcript Variant: This variant (1) represents the longest transcript. This variant can initiate translation from four non-AUG (CUG) sites, and also from a downstream, in-frame AUG. The isoform (a) represented in this RefSeq is derived from the 5'-most CUG start codon. This is the longest isoform. CCDS Note: This CCDS representation starts at an upstream non-AUG (CUG) translation start codon that is supported by conservation data and experimental evidence, including that in PMIDs:11352659, 11563986 and 11731620. Most of the publicly available long mRNAs at this locus do not extend far enough 5' to include the CUG start codon, including S85192.1 which supports this variant. However, BC058855.1, several unspliced partial transcripts and the aforementioned publications all support transcription initiation further upstream. Hence, this CCDS representation includes the extended 5' CDS. Alternative translation initiation from a downstream AUG codon (Met-181), which results in a shorter isoform with a predicted signal peptide, has also been shown for this gene. The shorter isoform encoded by this variant is represented by CCDS 55010.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: S85192.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2145240 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 11352659, 11563986, 11731620 ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
    CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC
    GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC
    TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG
    AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG
    AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG
    GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC
    CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC
    CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC
    ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT
    GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG
    GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC
    ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG
    ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC
    AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG
    AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA
    AAAAAATCAG TTCGAGGAAA GGGAAAGGGG CAAAAACGAA AGCGCAAGAA ATCCCGGTAT
    AAGTCCTGGA GCGTGTACGT TGGTGCCCGC TGCTGTCTAA TGCCCTGGAG CCTCCCTGGC
    CCCCATCCCT GTGGGCCTTG CTCAGAGCGG AGAAAGCATT TGTTTGTACA AGATCCGCAG
    ACGTGTAAAT GTTCCTGCAA AAACACAGAC TCGCGTTGCA AGGCGAGGCA GCTTGAGTTA
    AACGAACGTA CTTGCAGATG TGACAAGCCG AGGCGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001020537.2
    CDS499..1737
    Translation

    Target ORF information:

    RefSeq Version NM_001025366.2
    Organism Homo sapiens(human)
    Definition Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001025366.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
    CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC
    GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC
    TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG
    AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG
    AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG
    GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC
    CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC
    CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC
    ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT
    GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG
    GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC
    ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG
    ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC
    AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG
    AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA
    AAAAAATCAG TTCGAGGAAA GGGAAAGGGG CAAAAACGAA AGCGCAAGAA ATCCCGGTAT
    AAGTCCTGGA GCGTGTACGT TGGTGCCCGC TGCTGTCTAA TGCCCTGGAG CCTCCCTGGC
    CCCCATCCCT GTGGGCCTTG CTCAGAGCGG AGAAAGCATT TGTTTGTACA AGATCCGCAG
    ACGTGTAAAT GTTCCTGCAA AAACACAGAC TCGCGTTGCA AGGCGAGGCA GCTTGAGTTA
    AACGAACGTA CTTGCAGATG TGACAAGCCG AGGCGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu26721
    Clone ID Related Accession (Same CDS sequence) NM_001025367.3 , NM_001025367.2
    Accession Version NM_001025367.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1170bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-22
    Organism Homo sapiens(human)
    Product vascular endothelial growth factor A isoform c
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA237741.1, CN256173.1, BU153227.1, AJ010438.1, AF024710.1, BF700556.1 and BM661679.1. On Jun 2, 2019 this sequence version replaced NM_001025367.2. Transcript Variant: This variant (3) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This variant can initiate translation from four non-AUG (CUG) sites, and also from a downstream, in-frame AUG. The isoform (c) represented in this RefSeq is derived from the 5'-most CUG start codon, and is shorter than isoform a. CCDS Note: This CCDS representation starts at an upstream non-AUG (CUG) translation start codon that is supported by conservation data and experimental evidence, including that in PMIDs:11352659, 11563986 and 11731620. Most of the publicly available long mRNAs at this locus do not extend far enough 5' to include the CUG start codon, including AJ010438.1 which supports this variant. However, BC058855.1, several unspliced partial transcripts and the aforementioned publications all support transcription initiation further upstream. Hence, this CCDS representation includes the extended 5' CDS. Alternative translation initiation from a downstream AUG codon (Met-181), which results in a shorter isoform with a predicted signal peptide, has also been shown for this gene. The shorter isoform encoded by this variant is represented by CCDS 55008.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ010438.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 11352659, 11563986, 11731620 ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
    CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC
    GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC
    TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG
    AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG
    AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG
    GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC
    CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC
    CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC
    ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT
    GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG
    GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC
    ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG
    ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC
    AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG
    AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA
    AAAAAATCAG TTCGAGGAAA GGGAAAGGGG CAAAAACGAA AGCGCAAGAA ATCCCGTCCC
    TGTGGGCCTT GCTCAGAGCG GAGAAAGCAT TTGTTTGTAC AAGATCCGCA GACGTGTAAA
    TGTTCCTGCA AAAACACAGA CTCGCGTTGC AAGGCGAGGC AGCTTGAGTT AAACGAACGT
    ACTTGCAGAT GTGACAAGCC GAGGCGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001020538.2
    CDS497..1666
    Translation

    Target ORF information:

    RefSeq Version NM_001025367.3
    Organism Homo sapiens(human)
    Definition Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001025367.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
    CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC
    GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC
    TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG
    AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG
    AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG
    GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC
    CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC
    CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC
    ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT
    GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG
    GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC
    ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG
    ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC
    AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG
    AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA
    AAAAAATCAG TTCGAGGAAA GGGAAAGGGG CAAAAACGAA AGCGCAAGAA ATCCCGTCCC
    TGTGGGCCTT GCTCAGAGCG GAGAAAGCAT TTGTTTGTAC AAGATCCGCA GACGTGTAAA
    TGTTCCTGCA AAAACACAGA CTCGCGTTGC AAGGCGAGGC AGCTTGAGTT AAACGAACGT
    ACTTGCAGAT GTGACAAGCC GAGGCGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu26721
    Clone ID Related Accession (Same CDS sequence) NM_001025367.3 , NM_001025367.2
    Accession Version NM_001025367.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1170bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-03
    Organism Homo sapiens(human)
    Product vascular endothelial growth factor A isoform c
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA237741.1, CN256173.1, BU153227.1, AJ010438.1, AF024710.1, BF700556.1 and BM661679.1. On Jan 21, 2010 this sequence version replaced NM_001025367.1. Transcript Variant: This variant (3) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This variant can initiate translation from four non-AUG (CUG) sites, and also from a downstream, in-frame AUG. The isoform (c) represented in this RefSeq is derived from the 5'-most CUG start codon, and is shorter than isoform a. CCDS Note: This CCDS representation starts at an upstream non-AUG (CUG) translation start codon that is supported by conservation data and experimental evidence, including that in PMIDs:11352659, 11563986 and 11731620. Most of the publicly available long mRNAs at this locus do not extend far enough 5' to include the CUG start codon, including AJ010438.1 which supports this variant. However, BC058855.1, several unspliced partial transcripts and the aforementioned publications all support transcription initiation further upstream. Hence, this CCDS representation includes the extended 5' CDS. Alternative translation initiation from a downstream AUG codon (Met-181), which results in a shorter isoform with a predicted signal peptide, has also been shown for this gene. The shorter isoform encoded by this variant is represented by CCDS 55008.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ010438.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 11352659, 11563986, 11731620 ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
    CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC
    GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC
    TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG
    AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG
    AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG
    GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC
    CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC
    CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC
    ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT
    GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG
    GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC
    ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG
    ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC
    AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG
    AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA
    AAAAAATCAG TTCGAGGAAA GGGAAAGGGG CAAAAACGAA AGCGCAAGAA ATCCCGTCCC
    TGTGGGCCTT GCTCAGAGCG GAGAAAGCAT TTGTTTGTAC AAGATCCGCA GACGTGTAAA
    TGTTCCTGCA AAAACACAGA CTCGCGTTGC AAGGCGAGGC AGCTTGAGTT AAACGAACGT
    ACTTGCAGAT GTGACAAGCC GAGGCGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001020538.2
    CDS499..1668
    Translation

    Target ORF information:

    RefSeq Version NM_001025367.2
    Organism Homo sapiens(human)
    Definition Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001025367.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
    CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC
    GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC
    TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG
    AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG
    AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG
    GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC
    CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC
    CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC
    ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT
    GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG
    GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC
    ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG
    ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC
    AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG
    AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA
    AAAAAATCAG TTCGAGGAAA GGGAAAGGGG CAAAAACGAA AGCGCAAGAA ATCCCGTCCC
    TGTGGGCCTT GCTCAGAGCG GAGAAAGCAT TTGTTTGTAC AAGATCCGCA GACGTGTAAA
    TGTTCCTGCA AAAACACAGA CTCGCGTTGC AAGGCGAGGC AGCTTGAGTT AAACGAACGT
    ACTTGCAGAT GTGACAAGCC GAGGCGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu26819
    Clone ID Related Accession (Same CDS sequence) NM_001171623.1
    Accession Version NM_001171623.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 699bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-21
    Organism Homo sapiens(human)
    Product vascular endothelial growth factor A isoform i precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA237741.1, CN256173.1, BU153227.1, S85192.1, AF024710.1, BF700556.1 and BM661679.1. Transcript Variant: This variant (1) represents the longest transcript. This variant can initiate translation from four non-AUG (CUG) sites, and also from a downstream, in-frame AUG. The isoform (i, also referred to as VEGF206) represented in this RefSeq is derived from the AUG start codon, and is shorter at the N-terminus, compared to isoform a. CCDS Note: This CCDS, which is supported by the mRNA S85192.1, represents a VEGFA isoform derived from an AUG start codon. Alternative translation initiation from multiple upstream non-AUG(CUG) codons is also possible for this gene, as indicated in PMIDs:11352659, 11563986 and 11731620. The longest isoform encoded by this variant, which is derived from the 5'-most CUG start codon, is represented by CCDS 34457.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: S85192.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2145240 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## regulatory uORF :: PMID: 18304943 ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
    GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG
    GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC
    ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG
    ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC
    AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG
    AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA
    AAAAAATCAG TTCGAGGAAA GGGAAAGGGG CAAAAACGAA AGCGCAAGAA ATCCCGGTAT
    AAGTCCTGGA GCGTGTACGT TGGTGCCCGC TGCTGTCTAA TGCCCTGGAG CCTCCCTGGC
    CCCCATCCCT GTGGGCCTTG CTCAGAGCGG AGAAAGCATT TGTTTGTACA AGATCCGCAG
    ACGTGTAAAT GTTCCTGCAA AAACACAGAC TCGCGTTGCA AGGCGAGGCA GCTTGAGTTA
    AACGAACGTA CTTGCAGATG TGACAAGCCG AGGCGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001165094.1
    CDS1039..1737
    Translation

    Target ORF information:

    RefSeq Version NM_001171623.1
    Organism Homo sapiens(human)
    Definition Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001171623.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
    GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG
    GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC
    ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG
    ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC
    AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG
    AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA
    AAAAAATCAG TTCGAGGAAA GGGAAAGGGG CAAAAACGAA AGCGCAAGAA ATCCCGGTAT
    AAGTCCTGGA GCGTGTACGT TGGTGCCCGC TGCTGTCTAA TGCCCTGGAG CCTCCCTGGC
    CCCCATCCCT GTGGGCCTTG CTCAGAGCGG AGAAAGCATT TGTTTGTACA AGATCCGCAG
    ACGTGTAAAT GTTCCTGCAA AAACACAGAC TCGCGTTGCA AGGCGAGGCA GCTTGAGTTA
    AACGAACGTA CTTGCAGATG TGACAAGCCG AGGCGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu26825
    Clone ID Related Accession (Same CDS sequence) NM_001171628.1
    Accession Version NM_001171628.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 444bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-21
    Organism Homo sapiens(human)
    Product vascular endothelial growth factor A isoform n precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA237741.1, CN256173.1, BU153227.1, AF214570.1, AF024710.1, BF700556.1 and BM661679.1. Transcript Variant: This variant (6) lacks two alternate in-frame exons in the 3' coding region, compared to variant 1. This variant can initiate translation from four non-AUG (CUG) sites, and also from a downstream, in-frame AUG. The isoform (n, also referred to as VEGF121) represented in this RefSeq is derived from the AUG start codon, and is shorter than isoform a. CCDS Note: This CCDS, which is supported by the mRNA BC065522.1, represents a VEGFA isoform derived from an AUG start codon. Alternative translation initiation from multiple upstream non-AUG(CUG) codons is also possible for this gene, as indicated in PMIDs:11352659, 11563986 and 11731620. The longest isoform encoded by this variant, which is derived from the 5'-most CUG start codon, is represented by CCDS 47434.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC065522.1, ERR279836.8179.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## regulatory uORF :: PMID: 18304943 ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
    GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG
    GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC
    ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG
    ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC
    AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG
    AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA
    AAATGTGACA AGCCGAGGCG GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001165099.1
    CDS1039..1482
    Translation

    Target ORF information:

    RefSeq Version NM_001171628.1
    Organism Homo sapiens(human)
    Definition Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001171628.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
    GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG
    GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC
    ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG
    ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC
    AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG
    AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA
    AAATGTGACA AGCCGAGGCG GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu26865
    Clone ID Related Accession (Same CDS sequence) NM_001171627.1
    Accession Version NM_001171627.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 525bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-15
    Organism Homo sapiens(human)
    Product vascular endothelial growth factor A isoform m precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA237741.1, CN256173.1, BU153227.1, AF091352.1, AF024710.1, BF700556.1 and BM661679.1. Transcript Variant: This variant (5) lacks an alternate in-frame exon, and uses an alternate splice site that causes a frameshift in the 3' coding region, compared to variant 1. This variant can initiate translation from four non-AUG (CUG) sites, and also from a downstream, in-frame AUG. The isoform (m, also referred to as VEGF148) represented in this RefSeq is derived from the AUG start codon, and has a distinct C-terminus and is shorter than isoform a. CCDS Note: This CCDS, which is supported by the mRNA AF091352.1, represents a VEGFA isoform derived from an AUG start codon. Alternative translation initiation from multiple upstream non-AUG(CUG) codons is also possible for this gene, as indicated in PMIDs:11352659, 11563986 and 11731620. The longest isoform encoded by this variant, which is derived from the 5'-most CUG start codon, is represented by CCDS 47435.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF091352.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145122, SAMEA2145240 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## regulatory uORF :: PMID: 18304943 ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
    GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG
    GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC
    ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG
    ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC
    AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG
    AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA
    AATCCCTGTG GGCCTTGCTC AGAGCGGAGA AAGCATTTGT TTGTACAAGA TCCGCAGACG
    TGTAAATGTT CCTGCAAAAA CACAGACTCG CGTTGCAAGA TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001165098.1
    CDS1039..1563
    Translation

    Target ORF information:

    RefSeq Version NM_001171627.1
    Organism Homo sapiens(human)
    Definition Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001171627.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
    GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG
    GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC
    ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG
    ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC
    AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG
    AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA
    AATCCCTGTG GGCCTTGCTC AGAGCGGAGA AAGCATTTGT TTGTACAAGA TCCGCAGACG
    TGTAAATGTT CCTGCAAAAA CACAGACTCG CGTTGCAAGA TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu26938
    Clone ID Related Accession (Same CDS sequence) NM_001171625.1
    Accession Version NM_001171625.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 630bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-15
    Organism Homo sapiens(human)
    Product vascular endothelial growth factor A isoform k precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA237741.1, CN256173.1, BU153227.1, AJ010438.1, AF024710.1, BF700556.1 and BM661679.1. Transcript Variant: This variant (3) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This variant can initiate translation from four non-AUG (CUG) sites, and also from a downstream, in-frame AUG. The isoform (k, also referred to as VEGF183) represented in this RefSeq is derived from the AUG start codon, and is shorter than isoform a. CCDS Note: This CCDS, which is supported by the mRNA AJ010438.1, represents a VEGFA isoform derived from an AUG start codon. Alternative translation initiation from multiple upstream non-AUG(CUG) codons is also possible for this gene, as indicated in PMIDs:11352659, 11563986 and 11731620. The longest isoform encoded by this variant, which is derived from the 5'-most CUG start codon, is represented by CCDS 47433.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ010438.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## regulatory uORF :: PMID: 18304943 ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
    GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG
    GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC
    ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG
    ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC
    AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG
    AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA
    AAAAAATCAG TTCGAGGAAA GGGAAAGGGG CAAAAACGAA AGCGCAAGAA ATCCCGTCCC
    TGTGGGCCTT GCTCAGAGCG GAGAAAGCAT TTGTTTGTAC AAGATCCGCA GACGTGTAAA
    TGTTCCTGCA AAAACACAGA CTCGCGTTGC AAGGCGAGGC AGCTTGAGTT AAACGAACGT
    ACTTGCAGAT GTGACAAGCC GAGGCGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001165096.1
    CDS1039..1668
    Translation

    Target ORF information:

    RefSeq Version NM_001171625.1
    Organism Homo sapiens(human)
    Definition Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001171625.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
    GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG
    GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC
    ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG
    ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC
    AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG
    AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA
    AAAAAATCAG TTCGAGGAAA GGGAAAGGGG CAAAAACGAA AGCGCAAGAA ATCCCGTCCC
    TGTGGGCCTT GCTCAGAGCG GAGAAAGCAT TTGTTTGTAC AAGATCCGCA GACGTGTAAA
    TGTTCCTGCA AAAACACAGA CTCGCGTTGC AAGGCGAGGC AGCTTGAGTT AAACGAACGT
    ACTTGCAGAT GTGACAAGCC GAGGCGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu26944
    Clone ID Related Accession (Same CDS sequence) NM_001171624.1
    Accession Version NM_001171624.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 648bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-15
    Organism Homo sapiens(human)
    Product vascular endothelial growth factor A isoform j precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA237741.1, CN256173.1, BU153227.1, M27281.1, AF024710.1, BF700556.1 and BM661679.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This variant can initiate translation from four non-AUG (CUG) sites, and also from a downstream, in-frame AUG. The isoform (j, also referred to as VEGF189) represented in this RefSeq is derived from the AUG start codon, and is shorter than isoform a. CCDS Note: This CCDS, which is supported by the mRNA M27281.1, represents a VEGFA isoform derived from an AUG start codon. Alternative translation initiation from multiple upstream non-AUG(CUG) codons is also possible for this gene, as indicated in PMIDs:11352659, 11563986 and 11731620. The longest isoform encoded by this variant, which is derived from the 5'-most CUG start codon, is represented by CCDS 4907.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.367047.1, SRR1803617.494213.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## regulatory uORF :: PMID: 18304943 ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
    GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG
    GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC
    ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG
    ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC
    AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG
    AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA
    AAAAAATCAG TTCGAGGAAA GGGAAAGGGG CAAAAACGAA AGCGCAAGAA ATCCCGGTAT
    AAGTCCTGGA GCGTTCCCTG TGGGCCTTGC TCAGAGCGGA GAAAGCATTT GTTTGTACAA
    GATCCGCAGA CGTGTAAATG TTCCTGCAAA AACACAGACT CGCGTTGCAA GGCGAGGCAG
    CTTGAGTTAA ACGAACGTAC TTGCAGATGT GACAAGCCGA GGCGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001165095.1
    CDS1039..1686
    Translation

    Target ORF information:

    RefSeq Version NM_001171624.1
    Organism Homo sapiens(human)
    Definition Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001171624.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
    GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG
    GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC
    ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG
    ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC
    AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG
    AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA
    AAAAAATCAG TTCGAGGAAA GGGAAAGGGG CAAAAACGAA AGCGCAAGAA ATCCCGGTAT
    AAGTCCTGGA GCGTTCCCTG TGGGCCTTGC TCAGAGCGGA GAAAGCATTT GTTTGTACAA
    GATCCGCAGA CGTGTAAATG TTCCTGCAAA AACACAGACT CGCGTTGCAA GGCGAGGCAG
    CTTGAGTTAA ACGAACGTAC TTGCAGATGT GACAAGCCGA GGCGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu27131
    Clone ID Related Accession (Same CDS sequence) NM_001025368.2 , NM_001025368.3
    Accession Version NM_001025368.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1116bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-03
    Organism Homo sapiens(human)
    Product vascular endothelial growth factor A isoform d
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA237741.1, CN256173.1, BU153227.1, AB021221.1, AF024710.1, BF700556.1 and BM661679.1. On Jan 21, 2010 this sequence version replaced NM_001025368.1. Transcript Variant: This variant (4) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1. This variant can initiate translation from four non-AUG (CUG) sites, and also from a downstream, in-frame AUG. The isoform (d) represented in this RefSeq is derived from the 5'-most CUG start codon, and is shorter than isoform a. CCDS Note: This CCDS representation starts at an upstream non-AUG (CUG) translation start codon that is supported by conservation data and experimental evidence, including that in PMIDs:11352659, 11563986 and 11731620. This transcript variant is supported by the full-length mRNA BC058855.1. Alternative translation initiation from a downstream AUG codon (Met-181), which results in a shorter isoform with a predicted signal peptide, has also been shown for this gene. The shorter isoform encoded by this variant is represented by CCDS 55012.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC058855.1, AF022375.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 11352659, 11563986, 11731620 ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
    CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC
    GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC
    TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG
    AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG
    AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG
    GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC
    CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC
    CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC
    ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT
    GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG
    GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC
    ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG
    ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC
    AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG
    AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA
    AATCCCTGTG GGCCTTGCTC AGAGCGGAGA AAGCATTTGT TTGTACAAGA TCCGCAGACG
    TGTAAATGTT CCTGCAAAAA CACAGACTCG CGTTGCAAGG CGAGGCAGCT TGAGTTAAAC
    GAACGTACTT GCAGATGTGA CAAGCCGAGG CGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001020539.2
    CDS499..1614
    Translation

    Target ORF information:

    RefSeq Version NM_001025368.2
    Organism Homo sapiens(human)
    Definition Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001025368.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
    CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC
    GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC
    TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG
    AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG
    AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG
    GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC
    CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC
    CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC
    ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT
    GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG
    GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC
    ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG
    ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC
    AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG
    AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA
    AATCCCTGTG GGCCTTGCTC AGAGCGGAGA AAGCATTTGT TTGTACAAGA TCCGCAGACG
    TGTAAATGTT CCTGCAAAAA CACAGACTCG CGTTGCAAGG CGAGGCAGCT TGAGTTAAAC
    GAACGTACTT GCAGATGTGA CAAGCCGAGG CGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu27131
    Clone ID Related Accession (Same CDS sequence) NM_001025368.2 , NM_001025368.3
    Accession Version NM_001025368.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1116bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-07
    Organism Homo sapiens(human)
    Product vascular endothelial growth factor A isoform d
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA237741.1, CN256173.1, BU153227.1, AB021221.1, AF024710.1, BF700556.1 and BM661679.1. On Jun 2, 2019 this sequence version replaced NM_001025368.2. Transcript Variant: This variant (4) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1. This variant can initiate translation from four non-AUG (CUG) sites, and also from a downstream, in-frame AUG. The isoform (d) represented in this RefSeq is derived from the 5'-most CUG start codon, and is shorter than isoform a. CCDS Note: This CCDS representation starts at an upstream non-AUG (CUG) translation start codon that is supported by conservation data and experimental evidence, including that in PMIDs:11352659, 11563986 and 11731620. This transcript variant is supported by the full-length mRNA BC058855.1. Alternative translation initiation from a downstream AUG codon (Met-181), which results in a shorter isoform with a predicted signal peptide, has also been shown for this gene. The shorter isoform encoded by this variant is represented by CCDS 55012.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DRR138512.439172.1, BC058855.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 11352659, 11563986, 11731620 ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
    CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC
    GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC
    TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG
    AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG
    AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG
    GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC
    CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC
    CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC
    ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT
    GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG
    GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC
    ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG
    ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC
    AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG
    AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA
    AATCCCTGTG GGCCTTGCTC AGAGCGGAGA AAGCATTTGT TTGTACAAGA TCCGCAGACG
    TGTAAATGTT CCTGCAAAAA CACAGACTCG CGTTGCAAGG CGAGGCAGCT TGAGTTAAAC
    GAACGTACTT GCAGATGTGA CAAGCCGAGG CGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001020539.2
    CDS497..1612
    Translation

    Target ORF information:

    RefSeq Version NM_001025368.3
    Organism Homo sapiens(human)
    Definition Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001025368.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
    CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC
    GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC
    TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG
    AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG
    AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG
    GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC
    CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC
    CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC
    ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT
    GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG
    GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC
    ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG
    ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC
    AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG
    AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA
    AATCCCTGTG GGCCTTGCTC AGAGCGGAGA AAGCATTTGT TTGTACAAGA TCCGCAGACG
    TGTAAATGTT CCTGCAAAAA CACAGACTCG CGTTGCAAGG CGAGGCAGCT TGAGTTAAAC
    GAACGTACTT GCAGATGTGA CAAGCCGAGG CGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu27146
    Clone ID Related Accession (Same CDS sequence) NM_001171626.1
    Accession Version NM_001171626.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 576bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-15
    Organism Homo sapiens(human)
    Product vascular endothelial growth factor A isoform VEGF-A precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA237741.1, CN256173.1, BU153227.1, AB021221.1, AF024710.1, BF700556.1 and BM661679.1. Transcript Variant: This variant (4) lacks an alternate in-frame exon in the 3' coding region compared to variant 1. It can initiate translation from four non-AUG (CUG) sites, and also from an in-frame downstream AUG. This RefSeq represents isoform VEGF-A (also referred to as VEGF165 or VEGF-A165), which is derived from the AUG start codon, and is shorter than isoform a. This variant also encodes a C-terminally extended isoform (VEGF-Ax) that results from the use of an alternative in-frame, downstream translation termination codon (UGA). Isoform VEGF-Ax is represented in NM_001317010.1. CCDS Note: This CCDS, which is supported by the mRNA BC058855.1, represents a VEGFA isoform derived from an AUG start codon. Alternative translation initiation from multiple upstream non-AUG(CUG) codons is also possible for this gene, as indicated in PMIDs:11352659, 11563986 and 11731620. The longest isoform encoded by this variant, which is derived from the 5'-most CUG start codon, is represented by CCDS 34458.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC058855.1, AF022375.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## regulatory uORF :: PMID: 18304943 ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
    GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG
    GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC
    ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG
    ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC
    AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG
    AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA
    AATCCCTGTG GGCCTTGCTC AGAGCGGAGA AAGCATTTGT TTGTACAAGA TCCGCAGACG
    TGTAAATGTT CCTGCAAAAA CACAGACTCG CGTTGCAAGG CGAGGCAGCT TGAGTTAAAC
    GAACGTACTT GCAGATGTGA CAAGCCGAGG CGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001165097.1
    CDS1039..1614
    Translation

    Target ORF information:

    RefSeq Version NM_001171626.1
    Organism Homo sapiens(human)
    Definition Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001171626.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
    GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG
    GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC
    ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG
    ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC
    AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG
    AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA
    AATCCCTGTG GGCCTTGCTC AGAGCGGAGA AAGCATTTGT TTGTACAAGA TCCGCAGACG
    TGTAAATGTT CCTGCAAAAA CACAGACTCG CGTTGCAAGG CGAGGCAGCT TGAGTTAAAC
    GAACGTACTT GCAGATGTGA CAAGCCGAGG CGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu27630
    Clone ID Related Accession (Same CDS sequence) NM_001287044.1 , NM_001287044.2
    Accession Version NM_001287044.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 492bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-03
    Organism Homo sapiens(human)
    Product vascular endothelial growth factor A isoform s
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY066915.1, BX442364.2, BC065522.1 and BC058855.1. On Nov 23, 2013 this sequence version replaced XM_005249363.1. Transcript Variant: This variant (10) has an alternate 5' exon, which results in translation initiation at a downstream AUG start codon, and lacks an in-frame exon in the coding region, compared to variant 1. The resulting isoform (s) has a shorter N-terminus and lacks an internal segment, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.362344.1, SRR1803613.388738.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGGCAGAAG GAGGAGGGCA GAATCATCAC GAAGTGGTGA AGTTCATGGA TGTCTATCAG 
    CGCAGCTACT GCCATCCAAT CGAGACCCTG GTGGACATCT TCCAGGAGTA CCCTGATGAG
    ATCGAGTACA TCTTCAAGCC ATCCTGTGTG CCCCTGATGC GATGCGGGGG CTGCTGCAAT
    GACGAGGGCC TGGAGTGTGT GCCCACTGAG GAGTCCAACA TCACCATGCA GATTATGCGG
    ATCAAACCTC ACCAAGGCCA GCACATAGGA GAGATGAGCT TCCTACAGCA CAACAAATGT
    GAATGCAGAC CAAAGAAAGA TAGAGCAAGA CAAGAAAATC CCTGTGGGCC TTGCTCAGAG
    CGGAGAAAGC ATTTGTTTGT ACAAGATCCG CAGACGTGTA AATGTTCCTG CAAAAACACA
    GACTCGCGTT GCAAGGCGAG GCAGCTTGAG TTAAACGAAC GTACTTGCAG ATGTGACAAG
    CCGAGGCGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001273973.1
    CDS136..627
    Translation

    Target ORF information:

    RefSeq Version NM_001287044.1
    Organism Homo sapiens(human)
    Definition Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001287044.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGGCAGAAG GAGGAGGGCA GAATCATCAC GAAGTGGTGA AGTTCATGGA TGTCTATCAG 
    CGCAGCTACT GCCATCCAAT CGAGACCCTG GTGGACATCT TCCAGGAGTA CCCTGATGAG
    ATCGAGTACA TCTTCAAGCC ATCCTGTGTG CCCCTGATGC GATGCGGGGG CTGCTGCAAT
    GACGAGGGCC TGGAGTGTGT GCCCACTGAG GAGTCCAACA TCACCATGCA GATTATGCGG
    ATCAAACCTC ACCAAGGCCA GCACATAGGA GAGATGAGCT TCCTACAGCA CAACAAATGT
    GAATGCAGAC CAAAGAAAGA TAGAGCAAGA CAAGAAAATC CCTGTGGGCC TTGCTCAGAG
    CGGAGAAAGC ATTTGTTTGT ACAAGATCCG CAGACGTGTA AATGTTCCTG CAAAAACACA
    GACTCGCGTT GCAAGGCGAG GCAGCTTGAG TTAAACGAAC GTACTTGCAG ATGTGACAAG
    CCGAGGCGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu27630
    Clone ID Related Accession (Same CDS sequence) NM_001287044.1 , NM_001287044.2
    Accession Version NM_001287044.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 492bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-22
    Organism Homo sapiens(human)
    Product vascular endothelial growth factor A isoform s
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL136131.15, HY066915.1, BX442364.2, BC065522.1 and BC058855.1. On Jun 2, 2019 this sequence version replaced NM_001287044.1. Transcript Variant: This variant (10) has an alternate 5' exon, which results in translation initiation at a downstream AUG start codon, and lacks an in-frame exon in the coding region, compared to variant 1. The resulting isoform (s) has a shorter N-terminus and lacks an internal segment, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.362344.1, SRR1803613.388738.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGGCAGAAG GAGGAGGGCA GAATCATCAC GAAGTGGTGA AGTTCATGGA TGTCTATCAG 
    CGCAGCTACT GCCATCCAAT CGAGACCCTG GTGGACATCT TCCAGGAGTA CCCTGATGAG
    ATCGAGTACA TCTTCAAGCC ATCCTGTGTG CCCCTGATGC GATGCGGGGG CTGCTGCAAT
    GACGAGGGCC TGGAGTGTGT GCCCACTGAG GAGTCCAACA TCACCATGCA GATTATGCGG
    ATCAAACCTC ACCAAGGCCA GCACATAGGA GAGATGAGCT TCCTACAGCA CAACAAATGT
    GAATGCAGAC CAAAGAAAGA TAGAGCAAGA CAAGAAAATC CCTGTGGGCC TTGCTCAGAG
    CGGAGAAAGC ATTTGTTTGT ACAAGATCCG CAGACGTGTA AATGTTCCTG CAAAAACACA
    GACTCGCGTT GCAAGGCGAG GCAGCTTGAG TTAAACGAAC GTACTTGCAG ATGTGACAAG
    CCGAGGCGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001273973.1
    CDS153..644
    Translation

    Target ORF information:

    RefSeq Version NM_001287044.2
    Organism Homo sapiens(human)
    Definition Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001287044.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGGCAGAAG GAGGAGGGCA GAATCATCAC GAAGTGGTGA AGTTCATGGA TGTCTATCAG 
    CGCAGCTACT GCCATCCAAT CGAGACCCTG GTGGACATCT TCCAGGAGTA CCCTGATGAG
    ATCGAGTACA TCTTCAAGCC ATCCTGTGTG CCCCTGATGC GATGCGGGGG CTGCTGCAAT
    GACGAGGGCC TGGAGTGTGT GCCCACTGAG GAGTCCAACA TCACCATGCA GATTATGCGG
    ATCAAACCTC ACCAAGGCCA GCACATAGGA GAGATGAGCT TCCTACAGCA CAACAAATGT
    GAATGCAGAC CAAAGAAAGA TAGAGCAAGA CAAGAAAATC CCTGTGGGCC TTGCTCAGAG
    CGGAGAAAGC ATTTGTTTGT ACAAGATCCG CAGACGTGTA AATGTTCCTG CAAAAACACA
    GACTCGCGTT GCAAGGCGAG GCAGCTTGAG TTAAACGAAC GTACTTGCAG ATGTGACAAG
    CCGAGGCGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu14341
    Clone ID Related Accession (Same CDS sequence) NM_001204384.1
    Accession Version NM_001204384.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 516bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-21
    Organism Homo sapiens(human)
    Product vascular endothelial growth factor A isoform q precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL136131.15, M27281.1 and BC065522.1. Transcript Variant: This variant (9) uses an alternate in-frame splice site in the 3' coding region and lacks an alternate in-frame exon compared to variant 1. This variant can initiate translation from four non-AUG (CUG) sites, and also from a downstream, in-frame AUG. The isoform (q, also referred to as VEGF145) represented in this RefSeq is derived from the AUG start codon, and is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## regulatory uORF :: PMID: 18304943 ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
    GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG
    GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC
    ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG
    ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC
    AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG
    AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA
    AAAAAATCAG TTCGAGGAAA GGGAAAGGGG CAAAAACGAA AGCGCAAGAA ATCCCGGTAT
    AAGTCCTGGA GCGTATGTGA CAAGCCGAGG CGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001191313.1
    CDS1039..1554
    Translation

    Target ORF information:

    RefSeq Version NM_001204384.1
    Organism Homo sapiens(human)
    Definition Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001204384.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
    GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG
    GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC
    ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG
    ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC
    AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG
    AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA
    AAAAAATCAG TTCGAGGAAA GGGAAAGGGG CAAAAACGAA AGCGCAAGAA ATCCCGGTAT
    AAGTCCTGGA GCGTATGTGA CAAGCCGAGG CGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu26728
    Clone ID Related Accession (Same CDS sequence) NM_001171630.1
    Accession Version NM_001171630.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 414bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-15
    Organism Homo sapiens(human)
    Product vascular endothelial growth factor A isoform p precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA237741.1, CN256173.1, BU153227.1, DQ229900.1, AF024710.1, BF700556.1 and BM661679.1. Transcript Variant: This variant (8) lacks three alternate in-frame exons in the 3' coding region, compared to variant 1. This variant can initiate translation from four non-AUG (CUG) sites, and also from a downstream, in-frame AUG. The isoform (p, also referred to as VEGF111) represented in this RefSeq is derived from the AUG start codon, and is shorter than isoform a. CCDS Note: This CCDS, which is supported by the mRNA DQ229900.1, represents a VEGFA isoform derived from an AUG start codon. Alternative translation initiation from multiple upstream non-AUG(CUG) codons is also possible for this gene, as indicated in PMIDs:11352659, 11563986 and 11731620. The longest isoform encoded by this variant, which is derived from the 5'-most CUG start codon, is represented by CCDS 55007.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ229900.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA2142680 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## regulatory uORF :: PMID: 18304943 ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
    GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG
    GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC
    ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG
    ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC
    AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG
    AGCTTCCTAC AGCACAACAA ATGTGAATGC AGATGTGACA AGCCGAGGCG GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001165101.1
    CDS1039..1452
    Translation

    Target ORF information:

    RefSeq Version NM_001171630.1
    Organism Homo sapiens(human)
    Definition Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001171630.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
    GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG
    GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC
    ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG
    ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC
    AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG
    AGCTTCCTAC AGCACAACAA ATGTGAATGC AGATGTGACA AGCCGAGGCG GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu102720
    Clone ID Related Accession (Same CDS sequence) NM_001317010.1
    Accession Version NM_001317010.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 642bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-15
    Organism Homo sapiens(human)
    Product vascular endothelial growth factor A isoform VEGF-Ax precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA237741.1, CN256173.1, BU153227.1, AB021221.1, AF024710.1, BF700556.1 and BM661679.1. Transcript Variant: This variant (4) is identical to NM_001171626.1; however, it uses an alternative in-frame, downstream translation termination codon (UGA), which results in a longer, C-terminally extended isoform (VEGF-Ax) compared to isoform VEGF-A (also referred to as VEGF165 or VEGF-A165). Unlike isoform VEGF-A, which is proangiogenic, VEGF-Ax exhibits antiangiogenic activity. As the upstream UGA stop codon has been shown to be recoded as serine (PMID:24949972), its location in the VEGF-Ax isoform (aa 192) is denoted by an 'S'. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC058855.1, AF022375.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## regulatory uORF :: PMID: 18304943 stop codon readthrough :: PMID: 24949972 ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
    GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG
    GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC
    ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG
    ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC
    AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG
    AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA
    AATCCCTGTG GGCCTTGCTC AGAGCGGAGA AAGCATTTGT TTGTACAAGA TCCGCAGACG
    TGTAAATGTT CCTGCAAAAA CACAGACTCG CGTTGCAAGG CGAGGCAGCT TGAGTTAAAC
    GAACGTACTT GCAGATGTGA CAAGCCGAGG CGGTGAGCCG GGCAGGAGGA AGGAGCCTCC
    CTCAGGGTTT CGGGAACCAG ATCTCTCACC AGGAAAGACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001303939.1
    CDS1039..1680
    Translation

    Target ORF information:

    RefSeq Version NM_001317010.1
    Organism Homo sapiens(human)
    Definition Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001317010.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
    GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG
    GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC
    ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG
    ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC
    AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG
    AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA
    AATCCCTGTG GGCCTTGCTC AGAGCGGAGA AAGCATTTGT TTGTACAAGA TCCGCAGACG
    TGTAAATGTT CCTGCAAAAA CACAGACTCG CGTTGCAAGG CGAGGCAGCT TGAGTTAAAC
    GAACGTACTT GCAGATGTGA CAAGCCGAGG CGGTGAGCCG GGCAGGAGGA AGGAGCCTCC
    CTCAGGGTTT CGGGAACCAG ATCTCTCACC AGGAAAGACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu14217
    Clone ID Related Accession (Same CDS sequence) NM_001204385.2 , NM_001204385.1
    Accession Version NM_001204385.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1056bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-22
    Organism Homo sapiens(human)
    Product vascular endothelial growth factor A isoform r
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL136131.15, M27281.1 and BC065522.1. On Jun 2, 2019 this sequence version replaced NM_001204385.1. Transcript Variant: This variant (9) uses an alternate in-frame splice site in the 3' coding region and lacks an alternate in-frame exon compared to variant 1. This variant can initiate translation from four non-AUG (CUG) sites, and also from a downstream, in-frame AUG. The isoform represented in this RefSeq (isoform r) is derived from the 5'-most CUG start codon, and is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 11352659, 11563986, 11731620 ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
    CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC
    GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC
    TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG
    AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG
    AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG
    GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC
    CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC
    CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC
    ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT
    GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG
    GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC
    ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG
    ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC
    AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG
    AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA
    AAAAAATCAG TTCGAGGAAA GGGAAAGGGG CAAAAACGAA AGCGCAAGAA ATCCCGGTAT
    AAGTCCTGGA GCGTATGTGA CAAGCCGAGG CGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001191314.1
    CDS497..1552
    Translation

    Target ORF information:

    RefSeq Version NM_001204385.2
    Organism Homo sapiens(human)
    Definition Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001204385.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
    CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC
    GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC
    TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG
    AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG
    AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG
    GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC
    CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC
    CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC
    ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT
    GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG
    GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC
    ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG
    ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC
    AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG
    AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA
    AAAAAATCAG TTCGAGGAAA GGGAAAGGGG CAAAAACGAA AGCGCAAGAA ATCCCGGTAT
    AAGTCCTGGA GCGTATGTGA CAAGCCGAGG CGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu14217
    Clone ID Related Accession (Same CDS sequence) NM_001204385.2 , NM_001204385.1
    Accession Version NM_001204385.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1056bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-03
    Organism Homo sapiens(human)
    Product vascular endothelial growth factor A isoform r
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL136131.15, M27281.1 and BC065522.1. Transcript Variant: This variant (9) uses an alternate in-frame splice site in the 3' coding region and lacks an alternate in-frame exon compared to variant 1. This variant can initiate translation from four non-AUG (CUG) sites, and also from a downstream, in-frame AUG. The isoform represented in this RefSeq (isoform r) is derived from the 5'-most CUG start codon, and is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 11352659, 11563986, 11731620 ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
    CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC
    GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC
    TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG
    AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG
    AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG
    GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC
    CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC
    CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC
    ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT
    GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG
    GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC
    ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG
    ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC
    AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG
    AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA
    AAAAAATCAG TTCGAGGAAA GGGAAAGGGG CAAAAACGAA AGCGCAAGAA ATCCCGGTAT
    AAGTCCTGGA GCGTATGTGA CAAGCCGAGG CGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001191314.1
    CDS499..1554
    Translation

    Target ORF information:

    RefSeq Version NM_001204385.1
    Organism Homo sapiens(human)
    Definition Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001204385.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
    CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC
    GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC
    TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG
    AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG
    AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG
    GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC
    CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC
    CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC
    ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT
    GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG
    GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC
    ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG
    ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC
    AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG
    AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA
    AAAAAATCAG TTCGAGGAAA GGGAAAGGGG CAAAAACGAA AGCGCAAGAA ATCCCGGTAT
    AAGTCCTGGA GCGTATGTGA CAAGCCGAGG CGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu26588
    Clone ID Related Accession (Same CDS sequence) NM_001025369.2 , NM_001025369.3
    Accession Version NM_001025369.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1065bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-03
    Organism Homo sapiens(human)
    Product vascular endothelial growth factor A isoform e
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA237741.1, CN256173.1, BU153227.1, AF091352.1, AF024710.1, BF700556.1 and BM661679.1. On Jan 21, 2010 this sequence version replaced NM_001025369.1. Transcript Variant: This variant (5) lacks an alternate in-frame exon, and uses an alternate splice site that causes a frameshift in the 3' coding region, compared to variant 1. This variant can initiate translation from four non-AUG (CUG) sites, and also from a downstream, in-frame AUG. The isoform (e) represented in this RefSeq is derived from the 5'-most CUG start codon, and has a distinct C-terminus and is shorter than isoform a. CCDS Note: This CCDS representation starts at an upstream non-AUG (CUG) translation start codon that is supported by conservation data and experimental evidence, including that in PMIDs:11352659, 11563986 and 11731620. Most of the publicly available long mRNAs at this locus do not extend far enough 5' to include the CUG start codon, including AF091352.1 which supports this variant. However, BC058855.1, several unspliced partial transcripts and the aforementioned publications all support transcription initiation further upstream. Hence, this CCDS representation includes the extended 5' CDS. Alternative translation initiation from a downstream AUG codon (Met-181), which results in a shorter isoform with a predicted signal peptide, has also been shown for this gene. The shorter isoform encoded by this variant is represented by CCDS 55011.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF091352.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145122, SAMEA2145240 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 11352659, 11563986, 11731620 ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
    CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC
    GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC
    TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG
    AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG
    AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG
    GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC
    CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC
    CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC
    ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT
    GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG
    GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC
    ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG
    ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC
    AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG
    AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA
    AATCCCTGTG GGCCTTGCTC AGAGCGGAGA AAGCATTTGT TTGTACAAGA TCCGCAGACG
    TGTAAATGTT CCTGCAAAAA CACAGACTCG CGTTGCAAGA TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001020540.2
    CDS499..1563
    Translation

    Target ORF information:

    RefSeq Version NM_001025369.2
    Organism Homo sapiens(human)
    Definition Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001025369.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
    CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC
    GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC
    TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG
    AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG
    AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG
    GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC
    CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC
    CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC
    ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT
    GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG
    GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC
    ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG
    ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC
    AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG
    AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA
    AATCCCTGTG GGCCTTGCTC AGAGCGGAGA AAGCATTTGT TTGTACAAGA TCCGCAGACG
    TGTAAATGTT CCTGCAAAAA CACAGACTCG CGTTGCAAGA TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu26588
    Clone ID Related Accession (Same CDS sequence) NM_001025369.2 , NM_001025369.3
    Accession Version NM_001025369.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1065bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-03
    Organism Homo sapiens(human)
    Product vascular endothelial growth factor A isoform e
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA237741.1, CN256173.1, BU153227.1, AF091352.1, AF024710.1, BF700556.1 and BM661679.1. On Jun 2, 2019 this sequence version replaced NM_001025369.2. Transcript Variant: This variant (5) lacks an alternate in-frame exon, and uses an alternate splice site that causes a frameshift in the 3' coding region, compared to variant 1. This variant can initiate translation from four non-AUG (CUG) sites, and also from a downstream, in-frame AUG. The isoform (e) represented in this RefSeq is derived from the 5'-most CUG start codon, and has a distinct C-terminus and is shorter than isoform a. CCDS Note: This CCDS representation starts at an upstream non-AUG (CUG) translation start codon that is supported by conservation data and experimental evidence, including that in PMIDs:11352659, 11563986 and 11731620. Most of the publicly available long mRNAs at this locus do not extend far enough 5' to include the CUG start codon, including AF091352.1 which supports this variant. However, BC058855.1, several unspliced partial transcripts and the aforementioned publications all support transcription initiation further upstream. Hence, this CCDS representation includes the extended 5' CDS. Alternative translation initiation from a downstream AUG codon (Met-181), which results in a shorter isoform with a predicted signal peptide, has also been shown for this gene. The shorter isoform encoded by this variant is represented by CCDS 55011.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF091352.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145122, SAMEA2145240 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 11352659, 11563986, 11731620 ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
    CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC
    GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC
    TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG
    AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG
    AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG
    GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC
    CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC
    CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC
    ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT
    GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG
    GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC
    ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG
    ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC
    AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG
    AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA
    AATCCCTGTG GGCCTTGCTC AGAGCGGAGA AAGCATTTGT TTGTACAAGA TCCGCAGACG
    TGTAAATGTT CCTGCAAAAA CACAGACTCG CGTTGCAAGA TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001020540.2
    CDS497..1561
    Translation

    Target ORF information:

    RefSeq Version NM_001025369.3
    Organism Homo sapiens(human)
    Definition Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001025369.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
    CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC
    GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC
    TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG
    AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG
    AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG
    GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC
    CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC
    CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC
    ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT
    GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG
    GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC
    ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG
    ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC
    AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG
    AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA
    AATCCCTGTG GGCCTTGCTC AGAGCGGAGA AAGCATTTGT TTGTACAAGA TCCGCAGACG
    TGTAAATGTT CCTGCAAAAA CACAGACTCG CGTTGCAAGA TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu26828
    Clone ID Related Accession (Same CDS sequence) NM_001025370.3 , NM_001025370.2
    Accession Version NM_001025370.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 984bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-03
    Organism Homo sapiens(human)
    Product vascular endothelial growth factor A isoform f
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA237741.1, CN256173.1, BU153227.1, AF214570.1, AF024710.1, BF700556.1 and BM661679.1. On Jun 2, 2019 this sequence version replaced NM_001025370.2. Transcript Variant: This variant (6) lacks two alternate in-frame exons in the 3' coding region, compared to variant 1. This variant can initiate translation from four non-AUG (CUG) sites, and also from a downstream, in-frame AUG. The isoform (f) represented in this RefSeq is derived from the 5'-most CUG start codon, and is shorter than isoform a. CCDS Note: This CCDS representation starts at an upstream non-AUG (CUG) translation start codon that is supported by conservation data and experimental evidence, including that in PMIDs:11352659, 11563986 and 11731620. Most of the publicly available long mRNAs at this locus do not extend far enough 5' to include the CUG start codon, including BC065522.1 which supports this variant. However, BC058855.1, several unspliced partial transcripts and the aforementioned publications all support transcription initiation further upstream. Hence, this CCDS representation includes the extended 5' CDS. Alternative translation initiation from a downstream AUG codon (Met-181), which results in a shorter isoform with a predicted signal peptide, has also been shown for this gene. The shorter isoform encoded by this variant is represented by CCDS 55014.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC065522.1, DRR138528.598114.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 11352659, 11563986, 11731620 ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
    CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC
    GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC
    TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG
    AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG
    AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG
    GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC
    CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC
    CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC
    ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT
    GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG
    GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC
    ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG
    ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC
    AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG
    AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA
    AAATGTGACA AGCCGAGGCG GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001020541.2
    CDS497..1480
    Translation

    Target ORF information:

    RefSeq Version NM_001025370.3
    Organism Homo sapiens(human)
    Definition Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001025370.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
    CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC
    GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC
    TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG
    AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG
    AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG
    GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC
    CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC
    CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC
    ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT
    GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG
    GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC
    ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG
    ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC
    AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG
    AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA
    AAATGTGACA AGCCGAGGCG GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu26828
    Clone ID Related Accession (Same CDS sequence) NM_001025370.3 , NM_001025370.2
    Accession Version NM_001025370.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 984bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-03
    Organism Homo sapiens(human)
    Product vascular endothelial growth factor A isoform f
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA237741.1, CN256173.1, BU153227.1, AF214570.1, AF024710.1, BF700556.1 and BM661679.1. On Jan 21, 2010 this sequence version replaced NM_001025370.1. Transcript Variant: This variant (6) lacks two alternate in-frame exons in the 3' coding region, compared to variant 1. This variant can initiate translation from four non-AUG (CUG) sites, and also from a downstream, in-frame AUG. The isoform (f) represented in this RefSeq is derived from the 5'-most CUG start codon, and is shorter than isoform a. CCDS Note: This CCDS representation starts at an upstream non-AUG (CUG) translation start codon that is supported by conservation data and experimental evidence, including that in PMIDs:11352659, 11563986 and 11731620. Most of the publicly available long mRNAs at this locus do not extend far enough 5' to include the CUG start codon, including BC065522.1 which supports this variant. However, BC058855.1, several unspliced partial transcripts and the aforementioned publications all support transcription initiation further upstream. Hence, this CCDS representation includes the extended 5' CDS. Alternative translation initiation from a downstream AUG codon (Met-181), which results in a shorter isoform with a predicted signal peptide, has also been shown for this gene. The shorter isoform encoded by this variant is represented by CCDS 55014.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC065522.1, ERR279836.8179.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 11352659, 11563986, 11731620 ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
    CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC
    GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC
    TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG
    AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG
    AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG
    GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC
    CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC
    CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC
    ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT
    GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG
    GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC
    ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG
    ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC
    AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG
    AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA
    AAATGTGACA AGCCGAGGCG GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001020541.2
    CDS499..1482
    Translation

    Target ORF information:

    RefSeq Version NM_001025370.2
    Organism Homo sapiens(human)
    Definition Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001025370.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
    CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC
    GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC
    TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG
    AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG
    AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG
    GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC
    CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC
    CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC
    ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT
    GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG
    GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC
    ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG
    ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC
    AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG
    AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA
    AAATGTGACA AGCCGAGGCG GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu26860
    Clone ID Related Accession (Same CDS sequence) NM_003376.5 , NM_003376.6
    Accession Version NM_003376.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1188bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-03
    Organism Homo sapiens(human)
    Product vascular endothelial growth factor A isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA237741.1, CN256173.1, BU153227.1, M27281.1, AF024710.1, BF700556.1 and BM661679.1. On Jan 21, 2010 this sequence version replaced NM_003376.4. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This variant can initiate translation from four non-AUG (CUG) sites, and also from a downstream, in-frame AUG. The isoform represented in this RefSeq (isoform b) is derived from the 5'-most CUG start codon, and is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.367047.1, SRR1803617.494213.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 11352659, 11563986, 11731620 ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
    CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC
    GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC
    TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG
    AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG
    AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG
    GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC
    CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC
    CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC
    ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT
    GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG
    GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC
    ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG
    ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC
    AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG
    AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA
    AAAAAATCAG TTCGAGGAAA GGGAAAGGGG CAAAAACGAA AGCGCAAGAA ATCCCGGTAT
    AAGTCCTGGA GCGTTCCCTG TGGGCCTTGC TCAGAGCGGA GAAAGCATTT GTTTGTACAA
    GATCCGCAGA CGTGTAAATG TTCCTGCAAA AACACAGACT CGCGTTGCAA GGCGAGGCAG
    CTTGAGTTAA ACGAACGTAC TTGCAGATGT GACAAGCCGA GGCGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_003367.4
    CDS499..1686
    Translation

    Target ORF information:

    RefSeq Version NM_003376.5
    Organism Homo sapiens(human)
    Definition Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_003376.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
    CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC
    GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC
    TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG
    AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG
    AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG
    GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC
    CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC
    CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC
    ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT
    GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG
    GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC
    ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG
    ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC
    AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG
    AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA
    AAAAAATCAG TTCGAGGAAA GGGAAAGGGG CAAAAACGAA AGCGCAAGAA ATCCCGGTAT
    AAGTCCTGGA GCGTTCCCTG TGGGCCTTGC TCAGAGCGGA GAAAGCATTT GTTTGTACAA
    GATCCGCAGA CGTGTAAATG TTCCTGCAAA AACACAGACT CGCGTTGCAA GGCGAGGCAG
    CTTGAGTTAA ACGAACGTAC TTGCAGATGT GACAAGCCGA GGCGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu26860
    Clone ID Related Accession (Same CDS sequence) NM_003376.5 , NM_003376.6
    Accession Version NM_003376.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1188bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-22
    Organism Homo sapiens(human)
    Product vascular endothelial growth factor A isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA237741.1, CN256173.1, BU153227.1, M27281.1, AF024710.1, BF700556.1 and BM661679.1. On Jun 2, 2019 this sequence version replaced NM_003376.5. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This variant can initiate translation from four non-AUG (CUG) sites, and also from a downstream, in-frame AUG. The isoform represented in this RefSeq (isoform b) is derived from the 5'-most CUG start codon, and is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.367047.1, SRR1803617.494213.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 11352659, 11563986, 11731620 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
    CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC
    GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC
    TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG
    AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG
    AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG
    GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC
    CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC
    CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC
    ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT
    GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG
    GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC
    ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG
    ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC
    AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG
    AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA
    AAAAAATCAG TTCGAGGAAA GGGAAAGGGG CAAAAACGAA AGCGCAAGAA ATCCCGGTAT
    AAGTCCTGGA GCGTTCCCTG TGGGCCTTGC TCAGAGCGGA GAAAGCATTT GTTTGTACAA
    GATCCGCAGA CGTGTAAATG TTCCTGCAAA AACACAGACT CGCGTTGCAA GGCGAGGCAG
    CTTGAGTTAA ACGAACGTAC TTGCAGATGT GACAAGCCGA GGCGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_003367.4
    CDS497..1684
    Translation

    Target ORF information:

    RefSeq Version NM_003376.6
    Organism Homo sapiens(human)
    Definition Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_003376.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
    CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC
    GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC
    TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG
    AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG
    AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG
    GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC
    CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC
    CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC
    ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT
    GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG
    GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC
    ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG
    ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC
    AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG
    AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA
    AAAAAATCAG TTCGAGGAAA GGGAAAGGGG CAAAAACGAA AGCGCAAGAA ATCCCGGTAT
    AAGTCCTGGA GCGTTCCCTG TGGGCCTTGC TCAGAGCGGA GAAAGCATTT GTTTGTACAA
    GATCCGCAGA CGTGTAAATG TTCCTGCAAA AACACAGACT CGCGTTGCAA GGCGAGGCAG
    CTTGAGTTAA ACGAACGTAC TTGCAGATGT GACAAGCCGA GGCGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu27292
    Clone ID Related Accession (Same CDS sequence) NM_001033756.2 , NM_001033756.3
    Accession Version NM_001033756.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1116bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-03
    Organism Homo sapiens(human)
    Product vascular endothelial growth factor A isoform g
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA237741.1, CN256173.1, BU153227.1, AF430806.1, AF024710.1, BF700556.1 and BM661679.1. On Jan 21, 2010 this sequence version replaced NM_001033756.1. Transcript Variant: This variant (7) lacks an alternate in-frame exon and uses an alternate splice site that causes a frameshift in the 3' coding region, compared to variant 1. This variant can initiate translation from four non-AUG (CUG) sites, and also from a downstream, in-frame AUG. The isoform (g) represented in this RefSeq is derived from the 5'-most CUG start codon, and has a distinct C-terminus and is shorter than isoform a. CCDS Note: This CCDS representation starts at an upstream non-AUG (CUG) translation start codon that is supported by conservation data and experimental evidence, including that in PMIDs:11352659, 11563986 and 11731620. Most of the publicly available long mRNAs at this locus do not extend far enough 5' to include the CUG start codon, including AF430806.1 which supports this variant. However, BC058855.1, several unspliced partial transcripts and the aforementioned publications all support transcription initiation further upstream. Hence, this CCDS representation includes the extended 5' CDS. Alternative translation initiation from a downstream AUG codon (Met-181), which results in a shorter isoform with a predicted signal peptide, has also been shown for this gene. The shorter isoform encoded by this variant is represented by CCDS 55013.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF430806.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 11352659, 11563986, 11731620 ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
    CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC
    GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC
    TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG
    AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG
    AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG
    GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC
    CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC
    CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC
    ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT
    GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG
    GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC
    ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG
    ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC
    AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG
    AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA
    AATCCCTGTG GGCCTTGCTC AGAGCGGAGA AAGCATTTGT TTGTACAAGA TCCGCAGACG
    TGTAAATGTT CCTGCAAAAA CACAGACTCG CGTTGCAAGG CGAGGCAGCT TGAGTTAAAC
    GAACGTACTT GCAGATCTCT CACCAGGAAA GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001028928.1
    CDS499..1614
    Translation

    Target ORF information:

    RefSeq Version NM_001033756.2
    Organism Homo sapiens(human)
    Definition Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001033756.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
    CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC
    GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC
    TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG
    AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG
    AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG
    GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC
    CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC
    CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC
    ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT
    GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG
    GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC
    ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG
    ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC
    AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG
    AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA
    AATCCCTGTG GGCCTTGCTC AGAGCGGAGA AAGCATTTGT TTGTACAAGA TCCGCAGACG
    TGTAAATGTT CCTGCAAAAA CACAGACTCG CGTTGCAAGG CGAGGCAGCT TGAGTTAAAC
    GAACGTACTT GCAGATCTCT CACCAGGAAA GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu27292
    Clone ID Related Accession (Same CDS sequence) NM_001033756.2 , NM_001033756.3
    Accession Version NM_001033756.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1116bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-03
    Organism Homo sapiens(human)
    Product vascular endothelial growth factor A isoform g
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA237741.1, CN256173.1, BU153227.1, AF430806.1, AF024710.1, BF700556.1 and BM661679.1. On Jun 2, 2019 this sequence version replaced NM_001033756.2. Transcript Variant: This variant (7) lacks an alternate in-frame exon and uses an alternate splice site that causes a frameshift in the 3' coding region, compared to variant 1. This variant can initiate translation from four non-AUG (CUG) sites, and also from a downstream, in-frame AUG. The isoform (g) represented in this RefSeq is derived from the 5'-most CUG start codon, and has a distinct C-terminus and is shorter than isoform a. CCDS Note: This CCDS representation starts at an upstream non-AUG (CUG) translation start codon that is supported by conservation data and experimental evidence, including that in PMIDs:11352659, 11563986 and 11731620. Most of the publicly available long mRNAs at this locus do not extend far enough 5' to include the CUG start codon, including AF430806.1 which supports this variant. However, BC058855.1, several unspliced partial transcripts and the aforementioned publications all support transcription initiation further upstream. Hence, this CCDS representation includes the extended 5' CDS. Alternative translation initiation from a downstream AUG codon (Met-181), which results in a shorter isoform with a predicted signal peptide, has also been shown for this gene. The shorter isoform encoded by this variant is represented by CCDS 55013.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF430806.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 11352659, 11563986, 11731620 ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
    CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC
    GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC
    TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG
    AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG
    AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG
    GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC
    CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC
    CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC
    ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT
    GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG
    GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC
    ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG
    ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC
    AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG
    AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA
    AATCCCTGTG GGCCTTGCTC AGAGCGGAGA AAGCATTTGT TTGTACAAGA TCCGCAGACG
    TGTAAATGTT CCTGCAAAAA CACAGACTCG CGTTGCAAGG CGAGGCAGCT TGAGTTAAAC
    GAACGTACTT GCAGATCTCT CACCAGGAAA GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001028928.1
    CDS497..1612
    Translation

    Target ORF information:

    RefSeq Version NM_001033756.3
    Organism Homo sapiens(human)
    Definition Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001033756.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
    CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC
    GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC
    TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG
    AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG
    AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG
    GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC
    CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC
    CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC
    ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT
    GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG
    GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC
    ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG
    ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC
    AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG
    AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA
    AATCCCTGTG GGCCTTGCTC AGAGCGGAGA AAGCATTTGT TTGTACAAGA TCCGCAGACG
    TGTAAATGTT CCTGCAAAAA CACAGACTCG CGTTGCAAGG CGAGGCAGCT TGAGTTAAAC
    GAACGTACTT GCAGATCTCT CACCAGGAAA GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu27323
    Clone ID Related Accession (Same CDS sequence) NM_001171622.1 , NM_001171622.2
    Accession Version NM_001171622.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 954bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-03
    Organism Homo sapiens(human)
    Product vascular endothelial growth factor A isoform h
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA237741.1, CN256173.1, BU153227.1, DQ229900.1, AF024710.1, BF700556.1 and BM661679.1. Transcript Variant: This variant (8) lacks three alternate in-frame exons in the 3' coding region, compared to variant 1. This variant can initiate translation from four non-AUG (CUG) sites, and also from a downstream, in-frame AUG. The isoform (h) represented in this RefSeq is derived from the 5'-most CUG start codon, and is shorter than isoform a. CCDS Note: This CCDS representation starts at an upstream non-AUG (CUG) translation start codon that is supported by conservation data and experimental evidence, including that in PMIDs:11352659, 11563986 and 11731620. Most of the publicly available long mRNAs at this locus do not extend far enough 5' to include the CUG start codon, including DQ229900.1 which supports this variant. However, BC058855.1, several unspliced partial transcripts and the aforementioned publications all support transcription initiation further upstream. Hence, this CCDS representation includes the extended 5' CDS. Alternative translation initiation from a downstream AUG codon (Met-181), which results in a shorter isoform with a predicted signal peptide, has also been shown for this gene. The shorter isoform encoded by this variant is represented by CCDS 55015.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ229900.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA2142680 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 11352659, 11563986, 11731620 ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
    CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC
    GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC
    TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG
    AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG
    AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG
    GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC
    CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC
    CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC
    ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT
    GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG
    GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC
    ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG
    ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC
    AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG
    AGCTTCCTAC AGCACAACAA ATGTGAATGC AGATGTGACA AGCCGAGGCG GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001165093.1
    CDS499..1452
    Translation

    Target ORF information:

    RefSeq Version NM_001171622.1
    Organism Homo sapiens(human)
    Definition Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001171622.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
    CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC
    GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC
    TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG
    AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG
    AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG
    GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC
    CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC
    CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC
    ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT
    GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG
    GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC
    ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG
    ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC
    AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG
    AGCTTCCTAC AGCACAACAA ATGTGAATGC AGATGTGACA AGCCGAGGCG GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu27323
    Clone ID Related Accession (Same CDS sequence) NM_001171622.1 , NM_001171622.2
    Accession Version NM_001171622.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 954bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-03
    Organism Homo sapiens(human)
    Product vascular endothelial growth factor A isoform h
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA237741.1, CN256173.1, BU153227.1, DQ229900.1, AF024710.1, BF700556.1 and BM661679.1. On Jun 2, 2019 this sequence version replaced NM_001171622.1. Transcript Variant: This variant (8) lacks three alternate in-frame exons in the 3' coding region, compared to variant 1. This variant can initiate translation from four non-AUG (CUG) sites, and also from a downstream, in-frame AUG. The isoform (h) represented in this RefSeq is derived from the 5'-most CUG start codon, and is shorter than isoform a. CCDS Note: This CCDS representation starts at an upstream non-AUG (CUG) translation start codon that is supported by conservation data and experimental evidence, including that in PMIDs:11352659, 11563986 and 11731620. Most of the publicly available long mRNAs at this locus do not extend far enough 5' to include the CUG start codon, including DQ229900.1 which supports this variant. However, BC058855.1, several unspliced partial transcripts and the aforementioned publications all support transcription initiation further upstream. Hence, this CCDS representation includes the extended 5' CDS. Alternative translation initiation from a downstream AUG codon (Met-181), which results in a shorter isoform with a predicted signal peptide, has also been shown for this gene. The shorter isoform encoded by this variant is represented by CCDS 55015.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ229900.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA2142680 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 11352659, 11563986, 11731620 ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
    CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC
    GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC
    TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG
    AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG
    AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG
    GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC
    CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC
    CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC
    ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT
    GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG
    GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC
    ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG
    ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC
    AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG
    AGCTTCCTAC AGCACAACAA ATGTGAATGC AGATGTGACA AGCCGAGGCG GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001165093.1
    CDS497..1450
    Translation

    Target ORF information:

    RefSeq Version NM_001171622.2
    Organism Homo sapiens(human)
    Definition Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001171622.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
    CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC
    GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC
    TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG
    AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG
    AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG
    GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC
    CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC
    CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC
    ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT
    GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG
    GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC
    ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG
    ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC
    AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG
    AGCTTCCTAC AGCACAACAA ATGTGAATGC AGATGTGACA AGCCGAGGCG GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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