CACNA1E gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
NM_001191123.3	NP_001178052.1	voltage-dependent R-type calcium channel subunit alpha-1E
XM_010813517.2	XP_010811819.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X1
XM_015475247.1	XP_015330733.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X2
XM_015475248.1	XP_015330734.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X3
NM_001191123.1	NP_001178052.1	voltage-dependent R-type calcium channel subunit alpha-1E
XM_015475249.1	XP_015330735.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X4
XM_015475247.2	XP_015330733.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X2
XM_015475250.1	XP_015330736.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X5
NM_001191123.2	NP_001178052.1	voltage-dependent R-type calcium channel subunit alpha-1E
XM_024976061.1	XP_024831829.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa45603	XM_010813517.2 Latest version!	Bos taurus calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	22-24	$1161.30 $1659.00
OBa93040	XM_015475250.1 Latest version!	Bos taurus calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa216174	NM_001191123.2 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 E (CACNA1E), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OBa238960	NM_001191123.3 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 E (CACNA1E), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa223446	XM_024976061.1 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa223447	XM_015475247.2 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa93037	XM_015475247.1 Latest version!	Bos taurus calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OBa93038	XM_015475248.1 Latest version!	Bos taurus calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OBa93039	XM_015475249.1 Latest version!	Bos taurus calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OBa01606	NM_001191123.1 Latest version!	Bos taurus calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

CACNA1E ( XM_010813517.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010811819.1 Bos taurus calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa45603
Clone ID Related Accession (Same CDS sequence)	XM_010813517.2
Accession Version	XM_010813517.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6936bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104440.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010813517.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGCT TCGGGGAGGC GGTGGTCGGC AGGCCGGGGT CGGGCGATGG AGACTCGGAC 
CAGAGCAGGA ACCGGCAAGG AACCCCCGTG CCGGCCTCCG GGCCGGCGGC CGCCTACAAG 
CAGTCGAAAG CGCAGAGGGC GCGGACTATG GCTTTGTACA ACCCTATTCC CGTCCGGCAG 
AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA 
TACGCCAAGA AGCTCATCGA TTGGCCGCCG TTTGAGTACA TGATCTTGGC CACCATCATT 
GCCAATTGCA TCGTCTTGGC CCTGGAGCAA CATCTTCCTG AGGATGACAA GACCCCAATG 
TCCCGCAGAC TGGAGAAGAC AGAACCCTAT TTCATTGGGA TCTTTTGCTT TGAAGCTGGG 
ATCAAAATCG TGGCCCTGGG GTTCATCTTC CATAAGGGAT CATACCTCCG CAATGGTTGG 
AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CTACTGCGGG AACCCACTTC 
AACACCCATG TGGACCTGAG GACCCTCCGG GCCGTGCGTG TCCTGAGGCC CCTGAAGCTC 
GTGTCGGGGA TACCTAGCCT ACAGATTGTG TTGAAATCCA TCATGAAGGC CATGGTGCCT 
CTCCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATCCTGA TGTTTGCTAT CATCGGCTTG 
GAGTTCTACA GTGGAAAGTT GCACCGAGCG TGCTTCATGA ACAATTCAGG TATTCTAGAA 
GGATTTGACC CCCCTCACCC ATGTGGCGTG CAGGGCTGCC CAGCTGGTTA TGAATGCCGG 
GACTGGATCG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCCGTGCTG 
ACCGTCTTCC AGTGTATCAC CATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT 
GCCTTAGGAG CCACCTGGAA TTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC 
TTTGTTCTCA ACCTTGTCCT GGGAGTGCTT TCTGGGGAAT TTGCCAAAGA GAGAGAGAGA 
GTGGAGAACC GGAGAGCCTT CATGAAACTG CGGCGCCAGC AGCAGATCGA GCGTGAGCTT 
AACGGGTACC GCGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTTGCTGA AGAAAACAAA 
AATGCTGGAA CGTCAGCCTT AGAAGTGCTT CGGAGGGCAA CCATCAAAAG GAGCCGGACC 
GAGGCCATGG CACGGGACTC CAGCGATGAG CACTGCGTGG ACATCTCCTC TGTGGGCACA 
CCTCTCGCCC GAGCCAGCAT CAAGAGCGCC AAGGTGGACG GGGCCTCCTA TTTCCGGCAC 
AAGGAGCGAC TTTTGCGGAT CTCTATCCGC CACATGGTTA AGTCCCAGGT GTTCTACTGG 
ATCGTGCTGA GCCTGGTGGC TCTCAACACC GCCTGTGTGG CCATCGTCCA CCACAACCAG 
CCCCAGTGGC TCACCCACCT CCTCTACTAT GCAGAGTTTC TGTTTCTGGG GCTCTTCCTT 
CTAGAGATGT CCCTGAAGAT GTACGGCATG GGGCCTCGCC TTTATTTTCA CTCTTCCTTC 
AACTGCTTTG ACTTTGGGGT CACAGTGGGC AGCATCTTTG AAGTAGTCTG GGCCATCTTC 
AGACCTGGTA CATCTTTCGG AATCAGTGTC CTGCGAGCTC TCCGGCTTCT AAGAATATTT 
AAAATAACCA AGTATTGGGC ATCCCTCCGG AATTTGGTGG TCTCCTTGAT GAGTTCCATG 
AAGTCTATCA TCAGTTTACT CTTCCTCCTT TTCCTCTTCA TTGTTGTCTT TGCTCTGCTA 
GGAATGCAGC TGTTTGGGGG CAGGTTCAAC TTTAATGATG GGACTCCTTC CGCAAACTTT 
GACACCTTCC CTGCAGCCAT CATGACTGTA TTCCAGATCC TGACGGGTGA GGACTGGAAT 
GAGGTGATGT ACAACGGGAT CCGCTCCCAG GGTGGGGTCA GCTCGGGCAT GTGGTCTGCG 
ATCTATTTCA TTGTGCTTAC CTTGTTTGGA AACTACACAC TGCTGAATGT GTTCTTGGCC 
ATCGCTGTGG ACAATCTGGC CAACGCCCAG GAGCTGACCA AGGATGAACA AGAAGAAGAG 
GAGGCCTTCA ACCAGAAGCA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA 
CCCAACATGC CTTCCATCGA AAGAGACAGA AGGAGAAGAC ACCACATGTC GATGTGGGAG 
CCACGCAGCA GCCACCTGAG GGAGCGGAGG CGCCGGCATC ACATGTCCGT GTGGGAGCAG 
CGAACCAGCC AGTTGAGGAG GCACATGCAG ATGTCCAGCC AGGAGGCCCT CAACAAGGAG 
GAGGCCCCGC CCATGAACCC CCTCAACCCC CTCAACCCGC TGAGCCCCCT CAACCCGCTC 
AGCGCGCACC CCAGCCTCTA CCGGAGACCC CGGGCCATCG AGGGGCTAGC CCTGGGCCTG 
GGGCTGGAGA AGTGTGAGGA AGAGCGCGTC AGCCGTGGGG GGTCCCTCAA AGGGGATGGA 
GGGGACTTGG CGAGCGCCCT GGACAGCCAG AGGAGCCCCC TGGCCCTGGG CAAACGGGAG 
CCACCCTGGC TGGCCCGGCC CTGTCACGGG AACTGTGACC CGACCCAGCC GGAGGCGGGC 
AGTGGGGAGA CGGTGGTGAC CTTCGGGGAC CGGGCCCGGC ACAGGCAGAG CCAGCGGCGC 
AGCCGGCACC GCCGCGTCAG GACCGAGGGC AGGGAGCCCT CCTCCACCTC CAGAAGCAGG 
TCTGCCAGCC AGGAGCGGAG TCTGGACGAA GCTGTGCCCA GCGAGGGGGA GAGGGACCGT 
GAGCCCAGGG CTGGCCATGG GGGCAAAGAA CCCATGATCC AGGAGGAAGA GCGAGAGCGA 
TGCCAGGACT TACGGAGGAC CAACAGTCTG ATGGTGCCCA GAGGCTCCGG GCTGGCAGGA 
GCCCTGGATG AGGCCGACAC ACCCCTTGTC CCACCCCAGC CGGAGCTGGA AGTGGGGAAG 
GACGCAGCCC TGACGGAGCA GGAGCCGGAG GGCAGCAGTG AGCAGGCTCT GCTGGGGGAC 
GTGAAGCTGG ACATAGGCCG GGTCAGCCAG AGCGAGCCCG ACCTCTCCTG CGTCACGGCC 
AACACGGACA AGGCCACCAC CGAGAGCACC AGCGTCACTG TCACCATCCC CGACGTGGGC 
CCCTTGGTGG ACTCAACCGT GGTGCACATC AGCAACAAGA CTGATGGAGA AGCCAGTCCC 
TTGAAAGAGG CAGAGATCAG AGAGGATGAG GAGGAGGTGG AGAAGAAGCA GCAGAAGAAG 
GAGAAGCGTG AGACGGGCAA GGCCATGGTG CCCCACAGCT CGATGTTCAT CTTCAGCACC 
ACCAACCCGA TCCGCAGGGC CTGCCACTAC ATCGTGAACC TGCGCTACTT CGAGATGTGC 
ATCCTCCTGG TGATCGCAGC CAGCAGCATC GCCCTGGCGG CCGAGGACCC CGTCCTGACC 
AACTCAGAGC GCAACAAAGT GCTGAGGTAT TTTGACTATG TGTTCACTGG CGTGTTCACC 
TTTGAGATGG TTATAAAGAT GATAGACCAG GGCTTGATTC TGCAGGACGG CTCCTACTTC 
CGAGACCTGT GGAACATCCT GGACTTCGTG GTGGTGGTTG GCGCATTGGT GGCCTTTGCT 
CTGGCGAATG CTTTGGGAAC TAACAAGGGC CGGGATATTA AGACCATCAA GTCCCTGAGG 
GTTCTCCGTG TCCTGAGGCC GCTGAAGACC ATCAAGCGCC TGCCCAAGCT CAAGGCTGTC 
TTTGACTGTG TGGTCACCTC CTTGAAAAAC GTCTTCAACA TACTCATTGT CTACAAGCTC 
TTCATGTTCA TCTTTGCTGT CATCGCAGTT CAGCTTTTCA AGGGCAAGTT CTTCTACTGC 
ACGGACAGTT CCAAGGACAC AGAGAAGGAG TGCATAGGCA ACTATGTAGA TCACGAGAAA 
AACAAGATGG AGGTGAAAGG CCGAGAGTGG AAGCGCCATG AATTCCATTA TGACAACATC 
ATCTGGGCTC TGCTGACCCT CTTCACTGTC TCCACAGGGG AAGGGTGGCC TCAGGTCTTG 
CAGCACTCGG TCGATGTGAC AGAGGAAGAC CGAGGCCCAA GCCGCAGCAA CCGGATGGAG 
ATGTCCATCT TTTATGTGGT CTACTTTGTG GTCTTCCCCT TCTTCTTTGT CAACATCTTT 
GTGGCTCTCA TCATCATCAC CTTCCAGGAG CAAGGGGACA AGATGATGGA AGAGTGCAGC 
CTGGAGAAGA ATGAGAGGGC ATGCATCGAC TTCGCCATCA GCGCCAAACC GCTCACCCGC 
TACATGCCCC AGAACAGGCA CACCTTCCAG TACCGCGTCT GGCACTTCGT GGTGTCTCCA 
TCCTTTGAGT ACACCATCAT GGCCATGATC GCCCTGAACA CCGTCGTGCT GATGATGAAG 
TACTACTCTG CTCCCTGTAC CTATGAGCTG GCCCTGAAGT ACCTAAATAT CGCCTTCACC 
ATGGTGTTTT CCCTGGAATG TGTCCTGAAG ATCATTGCAT TTGGCTTCTT GAACTATTTC 
CGAGACACCT GGAATATCTT TGACTTCATC ACTGTGATTG GCAGTATCAC AGAAATTATT 
CTGACGGACA GCAAGCTGGT GAACACTAGT GGCTTCAACA TGAGCTTTCT GAAGCTCTTC 
CGAGCGGCCC GCCTCATCAA GCTGCTGCGT CAGGGCTACA CCATCCGCAT TTTACTGTGG 
ACCTTCGTGC AGTCCTTTAA GGCCCTTCCC TATGTCTGCC TTCTGATCGC CATGCTTTTC 
TTCATCTATG CCATCATTGG GATGCAGGTC TTTGGAAACA TAAAGTTAGA TGAGGAGAGT 
CACATCAACC GGCACAACAA CTTCCGGAGT TTCTTTGGGT CCCTCATGCT GCTCTTCAGG 
AGTGCCACGG GTGAGGCCTG GCAGGAGATA ATGCTGTCGT GTCTCGGGGA GAAGGGCTGT 
GAGCCCGACA CCACTGCGCC GTCGGGGCAG AGCGAGAGCG AGCGCTGTGG CACCGACCTG 
GCCTACGTGT ACTTCGTCTC CTTCATCTTC TTCTGCTCCT TCCTGATGCT CAACCTGTTC 
GTGGCTGTCA TCATGGACAA CTTTGAGTAC CTGACTCGGG ACTCGTCCAT CCTGGGGCCT 
CACCATCTGG ACGAGTTTGT CCGAGTCTGG GCAGAGTATG ACAGAGCAGC ATGTGGCCGC 
ATCCATTACA CTGAGATGTA TGAAATGCTG ACTCTCATGT CACCGCCGCT AGGCCTCGGC 
AAGAGATGTC CCTCCAAGGT GGCATATAAG AGGTTGGTCT TGATGAACAT GCCAGTGGCC 
GAGGACATGA CCGTCCACTT CACTTCCACG CTGATGGCTC TAATCCGGAC AGCTCTGGAC 
ATTAAAATCG CCAAAGGCGG TGCAGACAGG CAGCAGCTAG ACTCAGAGCT CCAGAAGGAG 
ACCCTGGCCA TCTGGCCTCA CCTGTCCCAG AAGATGCTGG ATCTGCTCGT GCCCATGCCC 
AAAGCCTCTG ACCTGACTGT GGGCAAAATC TATGCAGCAA TGATGATCAT GGACTACTAC 
AAACAGAGCA AGGTGAAGAA GCAGAGGCAG CAGCTGGAGG AACAGAAAAA CGCACCCATG 
TTCCAGCGCA TGGAGCCCTC GTCTCTGCCT CAGGAGATCA TCGCTAATGC CAAAGCCCTG 
CCTTATCTCC AGCAGGACCC CGTTCCAGGC CTGAGTGGCC GGAGTGCATA CCCTTCGATG 
AGTCCACTCT CCCCGCAGGA AATATTCCAG TTGGCCTGTA TGGACCCAGC TGATGATGGA 
CAGTTCCAGG AACGACAATC TCTGGAGCCA GAGGTTAGTG AATTAAAAAG CGTGCAGCCC 
TCTAACCATG GCATCTACCT TCCTTCGGAC ACCCAGGAGC ATGCGGGCTC TGGGAAGGCA 
TCCTCTATGC CACGCCTGAC TGTGGATCCC CAGGTGGTGA CAGACCCCAG CTCCATGAGA 
CGTTCATTTT CCACCATTCG CGATAAGCGT TCAAATTCCT CGTGGCTGGA GGAATTCTCC 
ATGGAGCGAA GCAGCGAAAA CACCTACAAG TCCCGGCGCC GGAGTTACCA CTCCTCCCTG 
AGGCTGTCAG CCCACCGTCT GAACTCGGAC TCAGGCCACA AGTCCGACAC CCACCGCTCA 
GGGGGCAGAG AGCGGGGACG ATCGAAAGAG CGAAAGCATC TTCTCTCTCC TGACGTCTCC 
CGCTGCAACT CCGAGGAGCG AGGGACCCAG GCCGACTGGG AGTCCCCAGA GCGCCATCAG 
TCCCGGTCTC CCAGTGAAGG CAGGTCACAG ACCCCCAACA GACAGGGCAC GGGGTCCCTG 
AGTGAGAGCT CCATCCCCTC TGTCTCCGAC TCCAGCACCC CACGACGAAG TCGGCGGCAG 
CTCCCCCCAG TCCCTCCCAA GCCTCGGCCC CTCCTTTCCT ACAGCTCTCT GATCAGACAC 
GCTGGGAGCA TCTCCCCACC AGCTGATGGG AGCGAGGGTG GCTCCCCACT GACCTCCCAA 
GCTCTGGAGA GCAACGACGC CGGCCTGCCC GAGTCTTCCA ACCCCCGGCA GGGCCACCCC 
TCCCCACAGC GCTACATCTC CGAGCCCTAC CTGGCCCTGC ACGAAGACTC CCACGCCTCG 
GACTGCGGCG AGGAGGAGAC GCTCACCTTT GAAGCAGCCG TGGCCACCAG CCTGGGCCGG 
TCCAACACCA TCGGCTCGGC CCCGCCCCTG CGGCACAGCT GGCAGATGCC CAATGGGCAC 
TATCGGCGGC GGAGGCGCGG GGGGCCTGGA CCGGGCACGA CGTGCGGAGC CGTCAGCGAC 
CTCCTGAGTG ACACGGAAGA AGACGACAAA TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010811819.1
CDS	1..6936
Translation	MARFGEAVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECRDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNAGTSALEVLRRATIKRSRTEAMARDSSDEHCVDISSVGTPLARASIKSAKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSLVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLSAHPSLYRRPRAIEGLALGLGLEKCEEERVSRGGSLKGDGGDLASALDSQRSPLALGKREPPWLARPCHGNCDPTQPEAGSGETVVTFGDRARHRQSQRRSRHRRVRTEGREPSSTSRSRSASQERSLDEAVPSEGERDREPRAGHGGKEPMIQEEERERCQDLRRTNSLMVPRGSGLAGALDEADTPLVPPQPELEVGKDAALTEQEPEGSSEQALLGDVKLDIGRVSQSEPDLSCVTANTDKATTESTSVTVTIPDVGPLVDSTVVHISNKTDGEASPLKEAEIREDEEEVEKKQQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKIIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQSESERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVPGLSGRSAYPSMSPLSPQEIFQLACMDPADDGQFQERQSLEPEVSELKSVQPSNHGIYLPSDTQEHAGSGKASSMPRLTVDPQVVTDPSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERHQSRSPSEGRSQTPNRQGTGSLSESSIPSVSDSSTPRRSRRQLPPVPPKPRPLLSYSSLIRHAGSISPPADGSEGGSPLTSQALESNDAGLPESSNPRQGHPSPQRYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGTTCGAVSDLLSDTEEDDKC

Target ORF information:

RefSeq Version	XM_010813517.2
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010813517.2

ORF Insert Sequence:

ATGGCTCGCT TCGGGGAGGC GGTGGTCGGC AGGCCGGGGT CGGGCGATGG AGACTCGGAC 
CAGAGCAGGA ACCGGCAAGG AACCCCCGTG CCGGCCTCCG GGCCGGCGGC CGCCTACAAG 
CAGTCGAAAG CGCAGAGGGC GCGGACTATG GCTTTGTACA ACCCTATTCC CGTCCGGCAG 
AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA 
TACGCCAAGA AGCTCATCGA TTGGCCGCCG TTTGAGTACA TGATCTTGGC CACCATCATT 
GCCAATTGCA TCGTCTTGGC CCTGGAGCAA CATCTTCCTG AGGATGACAA GACCCCAATG 
TCCCGCAGAC TGGAGAAGAC AGAACCCTAT TTCATTGGGA TCTTTTGCTT TGAAGCTGGG 
ATCAAAATCG TGGCCCTGGG GTTCATCTTC CATAAGGGAT CATACCTCCG CAATGGTTGG 
AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CTACTGCGGG AACCCACTTC 
AACACCCATG TGGACCTGAG GACCCTCCGG GCCGTGCGTG TCCTGAGGCC CCTGAAGCTC 
GTGTCGGGGA TACCTAGCCT ACAGATTGTG TTGAAATCCA TCATGAAGGC CATGGTGCCT 
CTCCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATCCTGA TGTTTGCTAT CATCGGCTTG 
GAGTTCTACA GTGGAAAGTT GCACCGAGCG TGCTTCATGA ACAATTCAGG TATTCTAGAA 
GGATTTGACC CCCCTCACCC ATGTGGCGTG CAGGGCTGCC CAGCTGGTTA TGAATGCCGG 
GACTGGATCG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCCGTGCTG 
ACCGTCTTCC AGTGTATCAC CATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT 
GCCTTAGGAG CCACCTGGAA TTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC 
TTTGTTCTCA ACCTTGTCCT GGGAGTGCTT TCTGGGGAAT TTGCCAAAGA GAGAGAGAGA 
GTGGAGAACC GGAGAGCCTT CATGAAACTG CGGCGCCAGC AGCAGATCGA GCGTGAGCTT 
AACGGGTACC GCGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTTGCTGA AGAAAACAAA 
AATGCTGGAA CGTCAGCCTT AGAAGTGCTT CGGAGGGCAA CCATCAAAAG GAGCCGGACC 
GAGGCCATGG CACGGGACTC CAGCGATGAG CACTGCGTGG ACATCTCCTC TGTGGGCACA 
CCTCTCGCCC GAGCCAGCAT CAAGAGCGCC AAGGTGGACG GGGCCTCCTA TTTCCGGCAC 
AAGGAGCGAC TTTTGCGGAT CTCTATCCGC CACATGGTTA AGTCCCAGGT GTTCTACTGG 
ATCGTGCTGA GCCTGGTGGC TCTCAACACC GCCTGTGTGG CCATCGTCCA CCACAACCAG 
CCCCAGTGGC TCACCCACCT CCTCTACTAT GCAGAGTTTC TGTTTCTGGG GCTCTTCCTT 
CTAGAGATGT CCCTGAAGAT GTACGGCATG GGGCCTCGCC TTTATTTTCA CTCTTCCTTC 
AACTGCTTTG ACTTTGGGGT CACAGTGGGC AGCATCTTTG AAGTAGTCTG GGCCATCTTC 
AGACCTGGTA CATCTTTCGG AATCAGTGTC CTGCGAGCTC TCCGGCTTCT AAGAATATTT 
AAAATAACCA AGTATTGGGC ATCCCTCCGG AATTTGGTGG TCTCCTTGAT GAGTTCCATG 
AAGTCTATCA TCAGTTTACT CTTCCTCCTT TTCCTCTTCA TTGTTGTCTT TGCTCTGCTA 
GGAATGCAGC TGTTTGGGGG CAGGTTCAAC TTTAATGATG GGACTCCTTC CGCAAACTTT 
GACACCTTCC CTGCAGCCAT CATGACTGTA TTCCAGATCC TGACGGGTGA GGACTGGAAT 
GAGGTGATGT ACAACGGGAT CCGCTCCCAG GGTGGGGTCA GCTCGGGCAT GTGGTCTGCG 
ATCTATTTCA TTGTGCTTAC CTTGTTTGGA AACTACACAC TGCTGAATGT GTTCTTGGCC 
ATCGCTGTGG ACAATCTGGC CAACGCCCAG GAGCTGACCA AGGATGAACA AGAAGAAGAG 
GAGGCCTTCA ACCAGAAGCA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA 
CCCAACATGC CTTCCATCGA AAGAGACAGA AGGAGAAGAC ACCACATGTC GATGTGGGAG 
CCACGCAGCA GCCACCTGAG GGAGCGGAGG CGCCGGCATC ACATGTCCGT GTGGGAGCAG 
CGAACCAGCC AGTTGAGGAG GCACATGCAG ATGTCCAGCC AGGAGGCCCT CAACAAGGAG 
GAGGCCCCGC CCATGAACCC CCTCAACCCC CTCAACCCGC TGAGCCCCCT CAACCCGCTC 
AGCGCGCACC CCAGCCTCTA CCGGAGACCC CGGGCCATCG AGGGGCTAGC CCTGGGCCTG 
GGGCTGGAGA AGTGTGAGGA AGAGCGCGTC AGCCGTGGGG GGTCCCTCAA AGGGGATGGA 
GGGGACTTGG CGAGCGCCCT GGACAGCCAG AGGAGCCCCC TGGCCCTGGG CAAACGGGAG 
CCACCCTGGC TGGCCCGGCC CTGTCACGGG AACTGTGACC CGACCCAGCC GGAGGCGGGC 
AGTGGGGAGA CGGTGGTGAC CTTCGGGGAC CGGGCCCGGC ACAGGCAGAG CCAGCGGCGC 
AGCCGGCACC GCCGCGTCAG GACCGAGGGC AGGGAGCCCT CCTCCACCTC CAGAAGCAGG 
TCTGCCAGCC AGGAGCGGAG TCTGGACGAA GCTGTGCCCA GCGAGGGGGA GAGGGACCGT 
GAGCCCAGGG CTGGCCATGG GGGCAAAGAA CCCATGATCC AGGAGGAAGA GCGAGAGCGA 
TGCCAGGACT TACGGAGGAC CAACAGTCTG ATGGTGCCCA GAGGCTCCGG GCTGGCAGGA 
GCCCTGGATG AGGCCGACAC ACCCCTTGTC CCACCCCAGC CGGAGCTGGA AGTGGGGAAG 
GACGCAGCCC TGACGGAGCA GGAGCCGGAG GGCAGCAGTG AGCAGGCTCT GCTGGGGGAC 
GTGAAGCTGG ACATAGGCCG GGTCAGCCAG AGCGAGCCCG ACCTCTCCTG CGTCACGGCC 
AACACGGACA AGGCCACCAC CGAGAGCACC AGCGTCACTG TCACCATCCC CGACGTGGGC 
CCCTTGGTGG ACTCAACCGT GGTGCACATC AGCAACAAGA CTGATGGAGA AGCCAGTCCC 
TTGAAAGAGG CAGAGATCAG AGAGGATGAG GAGGAGGTGG AGAAGAAGCA GCAGAAGAAG 
GAGAAGCGTG AGACGGGCAA GGCCATGGTG CCCCACAGCT CGATGTTCAT CTTCAGCACC 
ACCAACCCGA TCCGCAGGGC CTGCCACTAC ATCGTGAACC TGCGCTACTT CGAGATGTGC 
ATCCTCCTGG TGATCGCAGC CAGCAGCATC GCCCTGGCGG CCGAGGACCC CGTCCTGACC 
AACTCAGAGC GCAACAAAGT GCTGAGGTAT TTTGACTATG TGTTCACTGG CGTGTTCACC 
TTTGAGATGG TTATAAAGAT GATAGACCAG GGCTTGATTC TGCAGGACGG CTCCTACTTC 
CGAGACCTGT GGAACATCCT GGACTTCGTG GTGGTGGTTG GCGCATTGGT GGCCTTTGCT 
CTGGCGAATG CTTTGGGAAC TAACAAGGGC CGGGATATTA AGACCATCAA GTCCCTGAGG 
GTTCTCCGTG TCCTGAGGCC GCTGAAGACC ATCAAGCGCC TGCCCAAGCT CAAGGCTGTC 
TTTGACTGTG TGGTCACCTC CTTGAAAAAC GTCTTCAACA TACTCATTGT CTACAAGCTC 
TTCATGTTCA TCTTTGCTGT CATCGCAGTT CAGCTTTTCA AGGGCAAGTT CTTCTACTGC 
ACGGACAGTT CCAAGGACAC AGAGAAGGAG TGCATAGGCA ACTATGTAGA TCACGAGAAA 
AACAAGATGG AGGTGAAAGG CCGAGAGTGG AAGCGCCATG AATTCCATTA TGACAACATC 
ATCTGGGCTC TGCTGACCCT CTTCACTGTC TCCACAGGGG AAGGGTGGCC TCAGGTCTTG 
CAGCACTCGG TCGATGTGAC AGAGGAAGAC CGAGGCCCAA GCCGCAGCAA CCGGATGGAG 
ATGTCCATCT TTTATGTGGT CTACTTTGTG GTCTTCCCCT TCTTCTTTGT CAACATCTTT 
GTGGCTCTCA TCATCATCAC CTTCCAGGAG CAAGGGGACA AGATGATGGA AGAGTGCAGC 
CTGGAGAAGA ATGAGAGGGC ATGCATCGAC TTCGCCATCA GCGCCAAACC GCTCACCCGC 
TACATGCCCC AGAACAGGCA CACCTTCCAG TACCGCGTCT GGCACTTCGT GGTGTCTCCA 
TCCTTTGAGT ACACCATCAT GGCCATGATC GCCCTGAACA CCGTCGTGCT GATGATGAAG 
TACTACTCTG CTCCCTGTAC CTATGAGCTG GCCCTGAAGT ACCTAAATAT CGCCTTCACC 
ATGGTGTTTT CCCTGGAATG TGTCCTGAAG ATCATTGCAT TTGGCTTCTT GAACTATTTC 
CGAGACACCT GGAATATCTT TGACTTCATC ACTGTGATTG GCAGTATCAC AGAAATTATT 
CTGACGGACA GCAAGCTGGT GAACACTAGT GGCTTCAACA TGAGCTTTCT GAAGCTCTTC 
CGAGCGGCCC GCCTCATCAA GCTGCTGCGT CAGGGCTACA CCATCCGCAT TTTACTGTGG 
ACCTTCGTGC AGTCCTTTAA GGCCCTTCCC TATGTCTGCC TTCTGATCGC CATGCTTTTC 
TTCATCTATG CCATCATTGG GATGCAGGTC TTTGGAAACA TAAAGTTAGA TGAGGAGAGT 
CACATCAACC GGCACAACAA CTTCCGGAGT TTCTTTGGGT CCCTCATGCT GCTCTTCAGG 
AGTGCCACGG GTGAGGCCTG GCAGGAGATA ATGCTGTCGT GTCTCGGGGA GAAGGGCTGT 
GAGCCCGACA CCACTGCGCC GTCGGGGCAG AGCGAGAGCG AGCGCTGTGG CACCGACCTG 
GCCTACGTGT ACTTCGTCTC CTTCATCTTC TTCTGCTCCT TCCTGATGCT CAACCTGTTC 
GTGGCTGTCA TCATGGACAA CTTTGAGTAC CTGACTCGGG ACTCGTCCAT CCTGGGGCCT 
CACCATCTGG ACGAGTTTGT CCGAGTCTGG GCAGAGTATG ACAGAGCAGC ATGTGGCCGC 
ATCCATTACA CTGAGATGTA TGAAATGCTG ACTCTCATGT CACCGCCGCT AGGCCTCGGC 
AAGAGATGTC CCTCCAAGGT GGCATATAAG AGGTTGGTCT TGATGAACAT GCCAGTGGCC 
GAGGACATGA CCGTCCACTT CACTTCCACG CTGATGGCTC TAATCCGGAC AGCTCTGGAC 
ATTAAAATCG CCAAAGGCGG TGCAGACAGG CAGCAGCTAG ACTCAGAGCT CCAGAAGGAG 
ACCCTGGCCA TCTGGCCTCA CCTGTCCCAG AAGATGCTGG ATCTGCTCGT GCCCATGCCC 
AAAGCCTCTG ACCTGACTGT GGGCAAAATC TATGCAGCAA TGATGATCAT GGACTACTAC 
AAACAGAGCA AGGTGAAGAA GCAGAGGCAG CAGCTGGAGG AACAGAAAAA CGCACCCATG 
TTCCAGCGCA TGGAGCCCTC GTCTCTGCCT CAGGAGATCA TCGCTAATGC CAAAGCCCTG 
CCTTATCTCC AGCAGGACCC CGTTCCAGGC CTGAGTGGCC GGAGTGCATA CCCTTCGATG 
AGTCCACTCT CCCCGCAGGA AATATTCCAG TTGGCCTGTA TGGACCCAGC TGATGATGGA 
CAGTTCCAGG AACGACAATC TCTGGAGCCA GAGGTTAGTG AATTAAAAAG CGTGCAGCCC 
TCTAACCATG GCATCTACCT TCCTTCGGAC ACCCAGGAGC ATGCGGGCTC TGGGAAGGCA 
TCCTCTATGC CACGCCTGAC TGTGGATCCC CAGGTGGTGA CAGACCCCAG CTCCATGAGA 
CGTTCATTTT CCACCATTCG CGATAAGCGT TCAAATTCCT CGTGGCTGGA GGAATTCTCC 
ATGGAGCGAA GCAGCGAAAA CACCTACAAG TCCCGGCGCC GGAGTTACCA CTCCTCCCTG 
AGGCTGTCAG CCCACCGTCT GAACTCGGAC TCAGGCCACA AGTCCGACAC CCACCGCTCA 
GGGGGCAGAG AGCGGGGACG ATCGAAAGAG CGAAAGCATC TTCTCTCTCC TGACGTCTCC 
CGCTGCAACT CCGAGGAGCG AGGGACCCAG GCCGACTGGG AGTCCCCAGA GCGCCATCAG 
TCCCGGTCTC CCAGTGAAGG CAGGTCACAG ACCCCCAACA GACAGGGCAC GGGGTCCCTG 
AGTGAGAGCT CCATCCCCTC TGTCTCCGAC TCCAGCACCC CACGACGAAG TCGGCGGCAG 
CTCCCCCCAG TCCCTCCCAA GCCTCGGCCC CTCCTTTCCT ACAGCTCTCT GATCAGACAC 
GCTGGGAGCA TCTCCCCACC AGCTGATGGG AGCGAGGGTG GCTCCCCACT GACCTCCCAA 
GCTCTGGAGA GCAACGACGC CGGCCTGCCC GAGTCTTCCA ACCCCCGGCA GGGCCACCCC 
TCCCCACAGC GCTACATCTC CGAGCCCTAC CTGGCCCTGC ACGAAGACTC CCACGCCTCG 
GACTGCGGCG AGGAGGAGAC GCTCACCTTT GAAGCAGCCG TGGCCACCAG CCTGGGCCGG 
TCCAACACCA TCGGCTCGGC CCCGCCCCTG CGGCACAGCT GGCAGATGCC CAATGGGCAC 
TATCGGCGGC GGAGGCGCGG GGGGCCTGGA CCGGGCACGA CGTGCGGAGC CGTCAGCGAC 
CTCCTGAGTG ACACGGAAGA AGACGACAAA TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1E ( XM_015475250.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330736.1 Bos taurus calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X5, mRNA.

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CloneID	OBa93040
Clone ID Related Accession (Same CDS sequence)	XM_015475250.1
Accession Version	XM_015475250.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104440.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGCT TCGGGGAGGC GGTGGTCGGC AGGCCGGGGT CGGGCGATGG AGACTCGGAC 
CAGAGCAGGA ACCGGCAAGG AACCCCCGTG CCGGCCTCCG GGCCGGCGGC CGCCTACAAG 
CAGTCGAAAG CGCAGAGGGC GCGGACTATG GCTTTGTACA ACCCTATTCC CGTCCGGCAG 
AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA 
TACGCCAAGA AGCTCATCGA TTGGCCGCCG TTTGAGTACA TGATCTTGGC CACCATCATT 
GCCAATTGCA TCGTCTTGGC CCTGGAGCAA CATCTTCCTG AGGATGACAA GACCCCAATG 
TCCCGCAGAC TGGAGAAGAC AGAACCCTAT TTCATTGGGA TCTTTTGCTT TGAAGCTGGG 
ATCAAAATCG TGGCCCTGGG GTTCATCTTC CATAAGGGAT CATACCTCCG CAATGGTTGG 
AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CTACTGCGGG AACCCACTTC 
AACACCCATG TGGACCTGAG GACCCTCCGG GCCGTGCGTG TCCTGAGGCC CCTGAAGCTC 
GTGTCGGGGA TACCTAGCCT ACAGATTGTG TTGAAATCCA TCATGAAGGC CATGGTGCCT 
CTCCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATCCTGA TGTTTGCTAT CATCGGCTTG 
GAGTTCTACA GTGGAAAGTT GCACCGAGCG TGCTTCATGA ACAATTCAGG TATTCTAGAA 
GGATTTGACC CCCCTCACCC ATGTGGCGTG CAGGGCTGCC CAGCTGGTTA TGAATGCCGG 
GACTGGATCG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCCGTGCTG 
ACCGTCTTCC AGTGTATCAC CATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT 
GCCTTAGGAG CCACCTGGAA TTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC 
TTTGTTCTCA ACCTTGTCCT GGGAGTGCTT TCTGGGGAAT TTGCCAAAGA GAGAGAGAGA 
GTGGAGAACC GGAGAGCCTT CATGAAACTG CGGCGCCAGC AGCAGATCGA GCGTGAGCTT 
AACGGGTACC GCGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTTGCTGA AGAAAACAAA 
AATGCTGGAA CGTCAGCCTT AGAAGTGCTT CGGAGGGCAA CCATCAAAAG GAGCCGGACC 
GAGGCCATGG CACGGGACTC CAGCGATGAG CACTGCGTGG ACATCTCCTC TGTGGGCACA 
CCTCTCGCCC GAGCCAGCAT CAAGAGCGCC AAGGTGGACG GGGCCTCCTA TTTCCGGCAC 
AAGGAGCGAC TTTTGCGGAT CTCTATCCGC CACATGGTTA AGTCCCAGGT GTTCTACTGG 
ATCGTGCTGA GCCTGGTGGC TCTCAACACC GCCTGTGTGG CCATCGTCCA CCACAACCAG 
CCCCAGTGGC TCACCCACCT CCTCTACTAT GCAGAGTTTC TGTTTCTGGG GCTCTTCCTT 
CTAGAGATGT CCCTGAAGAT GTACGGCATG GGGCCTCGCC TTTATTTTCA CTCTTCCTTC 
AACTGCTTTG ACTTTGGGGT CACAGTGGGC AGCATCTTTG AAGTAGTCTG GGCCATCTTC 
AGACCTGGTA CATCTTTCGG AATCAGTGTC CTGCGAGCTC TCCGGCTTCT AAGAATATTT 
AAAATAACCA AGTATTGGGC ATCCCTCCGG AATTTGGTGG TCTCCTTGAT GAGTTCCATG 
AAGTCTATCA TCAGTTTACT CTTCCTCCTT TTCCTCTTCA TTGTTGTCTT TGCTCTGCTA 
GGAATGCAGC TGTTTGGGGG CAGGTTCAAC TTTAATGATG GGACTCCTTC CGCAAACTTT 
GACACCTTCC CTGCAGCCAT CATGACTGTA TTCCAGATCC TGACGGGTGA GGACTGGAAT 
GAGGTGATGT ACAACGGGAT CCGCTCCCAG GGTGGGGTCA GCTCGGGCAT GTGGTCTGCG 
ATCTATTTCA TTGTGCTTAC CTTGTTTGGA AACTACACAC TGCTGAATGT GTTCTTGGCC 
ATCGCTGTGG ACAATCTGGC CAACGCCCAG GAGCTGACCA AGGATGAACA AGAAGAAGAG 
GAGGCCTTCA ACCAGAAGCA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA 
CCCAACATGC CTTCCATCGA AAGAGACAGA AGGAGAAGAC ACCACATGTC GATGTGGGAG 
CCACGCAGCA GCCACCTGAG GGAGCGGAGG CGCCGGCATC ACATGTCCGT GTGGGAGCAG 
CGAACCAGCC AGTTGAGGAG GCACATGCAG ATGTCCAGCC AGGAGGCCCT CAACAAGGAG 
GAGGCCCCGC CCATGAACCC CCTCAACCCC CTCAACCCGC TGAGCCCCCT CAACCCGCTC 
AGCGCGCACC CCAGCCTCTA CCGGAGACCC CGGGCCATCG AGGGGCTAGC CCTGGGCCTG 
GGGCTGGAGA AGTGTGAGGA AGAGCGCGTC AGCCGTGGGG GGTCCCTCAA AGGGGATGGA 
GGGGACTTGG CGAGCGCCCT GGACAGCCAG AGGAGCCCCC TGGCCCTGGG CAAACGGGAG 
CCACCCTGGC TGGCCCGGCC CTGTCACGGG AACTGTGACC CGACCCAGCC GGAGGCGGGC 
AGTGGGGAGA CGGTGGTGAC CTTCGGGGAC CGGGCCCGGC ACAGGCAGAG CCAGCGGCGC 
AGCCGGCACC GCCGCGTCAG GACCGAGGGC AGGGAGCCCT CCTCCACCTC CAGAAGCAGG 
TCTGCCAGCC AGGAGCGGAG TCTGGACGAA GCTGTGCCCA GCGAGGGGGA GAGGGACCGT 
GAGCCCAGGG CTGGCCATGG GGGCAAAGAA CCCATGATCC AGGAGGAAGA GCGAGAGCGA 
TGCCAGGACT TACGGAGGAC CAACAGTCTG ATGGTGCCCA GAGGCTCCGG GCTGGCAGGA 
GCCCTGGATG AGGCCGACAC ACCCCTTGTC CCACCCCAGC CGGAGCTGGA AGTGGGGAAG 
GACGCAGCCC TGACGGAGCA GGAGCCGGAG GGCAGCAGTG AGCAGGCTCT GCTGGGGGAC 
GTGAAGCTGG ACATAGGCCG GGTCAGCCAG AGCGAGCCCG ACCTCTCCTG CGTCACGGCC 
AACACGGACA AGGCCACCAC CGAGAGCACC AGCGTCACTG TCACCATCCC CGACGTGGGC 
CCCTTGGTGG ACTCAACCGT GGTGCACATC AGCAACAAGA CTGATGGAGA AGCCAGTCCC 
TTGAAAGAGG CAGAGATCAG AGAGGATGAG GAGGAGGTGG AGAAGAAGCA GCAGAAGAAG 
GAGAAGCGTG AGACGGGCAA GGCCATGGTG CCCCACAGCT CGATGTTCAT CTTCAGCACC 
ACCAACCCGA TCCGCAGGGC CTGCCACTAC ATCGTGAACC TGCGCTACTT CGAGATGTGC 
ATCCTCCTGG TGATCGCAGC CAGCAGCATC GCCCTGGCGG CCGAGGACCC CGTCCTGACC 
AACTCAGAGC GCAACAAAGT GCTGAGGTAT TTTGACTATG TGTTCACTGG CGTGTTCACC 
TTTGAGATGG TTATAAAGAT GATAGACCAG GGCTTGATTC TGCAGGACGG CTCCTACTTC 
CGAGACCTGT GGAACATCCT GGACTTCGTG GTGGTGGTTG GCGCATTGGT GGCCTTTGCT 
CTGGCGAATG CTTTGGGAAC TAACAAGGGC CGGGATATTA AGACCATCAA GTCCCTGAGG 
GTTCTCCGTG TCCTGAGGCC GCTGAAGACC ATCAAGCGCC TGCCCAAGCT CAAGGCTGTC 
TTTGACTGTG TGGTCACCTC CTTGAAAAAC GTCTTCAACA TACTCATTGT CTACAAGCTC 
TTCATGTTCA TCTTTGCTGT CATCGCAGTT CAGCTTTTCA AGGGCAAGTT CTTCTACTGC 
ACGGACAGTT CCAAGGACAC AGAGAAGGAG TGCATAGGCA ACTATGTAGA TCACGAGAAA 
AACAAGATGG AGGTGAAAGG CCGAGAGTGG AAGCGCCATG AATTCCATTA TGACAACATC 
ATCTGGGCTC TGCTGACCCT CTTCACTGTC TCCACAGGGG AAGGGTGGCC TCAGGTCTTG 
CAGCACTCGG TCGATGTGAC AGAGGAAGAC CGAGGCCCAA GCCGCAGCAA CCGGATGGAG 
ATGTCCATCT TTTATGTGGT CTACTTTGTG GTCTTCCCCT TCTTCTTTGT CAACATCTTT 
GTGGCTCTCA TCATCATCAC CTTCCAGGAG CAAGGGGACA AGATGATGGA AGAGTGCAGC 
CTGGAGAAGA ATGAGAGGGC ATGCATCGAC TTCGCCATCA GCGCCAAACC GCTCACCCGC 
TACATGCCCC AGAACAGGCA CACCTTCCAG TACCGCGTCT GGCACTTCGT GGTGTCTCCA 
TCCTTTGAGT ACACCATCAT GGCCATGATC GCCCTGAACA CCGTCGTGCT GATGATGAAG 
TACTACTCTG CTCCCTGTAC CTATGAGCTG GCCCTGAAGT ACCTAAATAT CGCCTTCACC 
ATGGTGTTTT CCCTGGAATG TGTCCTGAAG ATCATTGCAT TTGGCTTCTT GAACTATTTC 
CGAGACACCT GGAATATCTT TGACTTCATC ACTGTGATTG GCAGTATCAC AGAAATTATT 
CTGACGGACA GCAAGCTGGT GAACACTAGT GGCTTCAACA TGAGCTTTCT GAAGCTCTTC 
CGAGCGGCCC GCCTCATCAA GCTGCTGCGT CAGGGCTACA CCATCCGCAT TTTACTGTGG 
ACCTTCGTGC AGTCCTTTAA GGCCCTTCCC TATGTCTGCC TTCTGATCGC CATGCTTTTC 
TTCATCTATG CCATCATTGG GATGCAGGTC TTTGGAAACA TAAAGTTAGA TGAGGAGAGT 
CACATCAACC GGCACAACAA CTTCCGGAGT TTCTTTGGGT CCCTCATGCT GCTCTTCAGG 
AGTGCCACGG GTGAGGCCTG GCAGGAGATA ATGCTGTCGT GTCTCGGGGA GAAGGGCTGT 
GAGCCCGACA CCACTGCGCC GTCGGGGCAG AGCGAGAGCG AGCGCTGTGG CACCGACCTG 
GCCTACGTGT ACTTCGTCTC CTTCATCTTC TTCTGCTCCT TCCTGATGCT CAACCTGTTC 
GTGGCTGTCA TCATGGACAA CTTTGAGTAC CTGACTCGGG ACTCGTCCAT CCTGGGGCCT 
CACCATCTGG ACGAGTTTGT CCGAGTCTGG GCAGAGTATG ACAGAGCAGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330736.1
CDS	846..6119
Translation	MARFGEAVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECRDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNAGTSALEVLRRATIKRSRTEAMARDSSDEHCVDISSVGTPLARASIKSAKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSLVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLSAHPSLYRRPRAIEGLALGLGLEKCEEERVSRGGSLKGDGGDLASALDSQRSPLALGKREPPWLARPCHGNCDPTQPEAGSGETVVTFGDRARHRQSQRRSRHRRVRTEGREPSSTSRSRSASQERSLDEAVPSEGERDREPRAGHGGKEPMIQEEERERCQDLRRTNSLMVPRGSGLAGALDEADTPLVPPQPELEVGKDAALTEQEPEGSSEQALLGDVKLDIGRVSQSEPDLSCVTANTDKATTESTSVTVTIPDVGPLVDSTVVHISNKTDGEASPLKEAEIREDEEEVEKKQQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKIIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQSESERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAA

Target ORF information:

RefSeq Version	XM_015475250.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015475250.1

ORF Insert Sequence:

ATGGCTCGCT TCGGGGAGGC GGTGGTCGGC AGGCCGGGGT CGGGCGATGG AGACTCGGAC 
CAGAGCAGGA ACCGGCAAGG AACCCCCGTG CCGGCCTCCG GGCCGGCGGC CGCCTACAAG 
CAGTCGAAAG CGCAGAGGGC GCGGACTATG GCTTTGTACA ACCCTATTCC CGTCCGGCAG 
AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA 
TACGCCAAGA AGCTCATCGA TTGGCCGCCG TTTGAGTACA TGATCTTGGC CACCATCATT 
GCCAATTGCA TCGTCTTGGC CCTGGAGCAA CATCTTCCTG AGGATGACAA GACCCCAATG 
TCCCGCAGAC TGGAGAAGAC AGAACCCTAT TTCATTGGGA TCTTTTGCTT TGAAGCTGGG 
ATCAAAATCG TGGCCCTGGG GTTCATCTTC CATAAGGGAT CATACCTCCG CAATGGTTGG 
AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CTACTGCGGG AACCCACTTC 
AACACCCATG TGGACCTGAG GACCCTCCGG GCCGTGCGTG TCCTGAGGCC CCTGAAGCTC 
GTGTCGGGGA TACCTAGCCT ACAGATTGTG TTGAAATCCA TCATGAAGGC CATGGTGCCT 
CTCCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATCCTGA TGTTTGCTAT CATCGGCTTG 
GAGTTCTACA GTGGAAAGTT GCACCGAGCG TGCTTCATGA ACAATTCAGG TATTCTAGAA 
GGATTTGACC CCCCTCACCC ATGTGGCGTG CAGGGCTGCC CAGCTGGTTA TGAATGCCGG 
GACTGGATCG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCCGTGCTG 
ACCGTCTTCC AGTGTATCAC CATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT 
GCCTTAGGAG CCACCTGGAA TTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC 
TTTGTTCTCA ACCTTGTCCT GGGAGTGCTT TCTGGGGAAT TTGCCAAAGA GAGAGAGAGA 
GTGGAGAACC GGAGAGCCTT CATGAAACTG CGGCGCCAGC AGCAGATCGA GCGTGAGCTT 
AACGGGTACC GCGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTTGCTGA AGAAAACAAA 
AATGCTGGAA CGTCAGCCTT AGAAGTGCTT CGGAGGGCAA CCATCAAAAG GAGCCGGACC 
GAGGCCATGG CACGGGACTC CAGCGATGAG CACTGCGTGG ACATCTCCTC TGTGGGCACA 
CCTCTCGCCC GAGCCAGCAT CAAGAGCGCC AAGGTGGACG GGGCCTCCTA TTTCCGGCAC 
AAGGAGCGAC TTTTGCGGAT CTCTATCCGC CACATGGTTA AGTCCCAGGT GTTCTACTGG 
ATCGTGCTGA GCCTGGTGGC TCTCAACACC GCCTGTGTGG CCATCGTCCA CCACAACCAG 
CCCCAGTGGC TCACCCACCT CCTCTACTAT GCAGAGTTTC TGTTTCTGGG GCTCTTCCTT 
CTAGAGATGT CCCTGAAGAT GTACGGCATG GGGCCTCGCC TTTATTTTCA CTCTTCCTTC 
AACTGCTTTG ACTTTGGGGT CACAGTGGGC AGCATCTTTG AAGTAGTCTG GGCCATCTTC 
AGACCTGGTA CATCTTTCGG AATCAGTGTC CTGCGAGCTC TCCGGCTTCT AAGAATATTT 
AAAATAACCA AGTATTGGGC ATCCCTCCGG AATTTGGTGG TCTCCTTGAT GAGTTCCATG 
AAGTCTATCA TCAGTTTACT CTTCCTCCTT TTCCTCTTCA TTGTTGTCTT TGCTCTGCTA 
GGAATGCAGC TGTTTGGGGG CAGGTTCAAC TTTAATGATG GGACTCCTTC CGCAAACTTT 
GACACCTTCC CTGCAGCCAT CATGACTGTA TTCCAGATCC TGACGGGTGA GGACTGGAAT 
GAGGTGATGT ACAACGGGAT CCGCTCCCAG GGTGGGGTCA GCTCGGGCAT GTGGTCTGCG 
ATCTATTTCA TTGTGCTTAC CTTGTTTGGA AACTACACAC TGCTGAATGT GTTCTTGGCC 
ATCGCTGTGG ACAATCTGGC CAACGCCCAG GAGCTGACCA AGGATGAACA AGAAGAAGAG 
GAGGCCTTCA ACCAGAAGCA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA 
CCCAACATGC CTTCCATCGA AAGAGACAGA AGGAGAAGAC ACCACATGTC GATGTGGGAG 
CCACGCAGCA GCCACCTGAG GGAGCGGAGG CGCCGGCATC ACATGTCCGT GTGGGAGCAG 
CGAACCAGCC AGTTGAGGAG GCACATGCAG ATGTCCAGCC AGGAGGCCCT CAACAAGGAG 
GAGGCCCCGC CCATGAACCC CCTCAACCCC CTCAACCCGC TGAGCCCCCT CAACCCGCTC 
AGCGCGCACC CCAGCCTCTA CCGGAGACCC CGGGCCATCG AGGGGCTAGC CCTGGGCCTG 
GGGCTGGAGA AGTGTGAGGA AGAGCGCGTC AGCCGTGGGG GGTCCCTCAA AGGGGATGGA 
GGGGACTTGG CGAGCGCCCT GGACAGCCAG AGGAGCCCCC TGGCCCTGGG CAAACGGGAG 
CCACCCTGGC TGGCCCGGCC CTGTCACGGG AACTGTGACC CGACCCAGCC GGAGGCGGGC 
AGTGGGGAGA CGGTGGTGAC CTTCGGGGAC CGGGCCCGGC ACAGGCAGAG CCAGCGGCGC 
AGCCGGCACC GCCGCGTCAG GACCGAGGGC AGGGAGCCCT CCTCCACCTC CAGAAGCAGG 
TCTGCCAGCC AGGAGCGGAG TCTGGACGAA GCTGTGCCCA GCGAGGGGGA GAGGGACCGT 
GAGCCCAGGG CTGGCCATGG GGGCAAAGAA CCCATGATCC AGGAGGAAGA GCGAGAGCGA 
TGCCAGGACT TACGGAGGAC CAACAGTCTG ATGGTGCCCA GAGGCTCCGG GCTGGCAGGA 
GCCCTGGATG AGGCCGACAC ACCCCTTGTC CCACCCCAGC CGGAGCTGGA AGTGGGGAAG 
GACGCAGCCC TGACGGAGCA GGAGCCGGAG GGCAGCAGTG AGCAGGCTCT GCTGGGGGAC 
GTGAAGCTGG ACATAGGCCG GGTCAGCCAG AGCGAGCCCG ACCTCTCCTG CGTCACGGCC 
AACACGGACA AGGCCACCAC CGAGAGCACC AGCGTCACTG TCACCATCCC CGACGTGGGC 
CCCTTGGTGG ACTCAACCGT GGTGCACATC AGCAACAAGA CTGATGGAGA AGCCAGTCCC 
TTGAAAGAGG CAGAGATCAG AGAGGATGAG GAGGAGGTGG AGAAGAAGCA GCAGAAGAAG 
GAGAAGCGTG AGACGGGCAA GGCCATGGTG CCCCACAGCT CGATGTTCAT CTTCAGCACC 
ACCAACCCGA TCCGCAGGGC CTGCCACTAC ATCGTGAACC TGCGCTACTT CGAGATGTGC 
ATCCTCCTGG TGATCGCAGC CAGCAGCATC GCCCTGGCGG CCGAGGACCC CGTCCTGACC 
AACTCAGAGC GCAACAAAGT GCTGAGGTAT TTTGACTATG TGTTCACTGG CGTGTTCACC 
TTTGAGATGG TTATAAAGAT GATAGACCAG GGCTTGATTC TGCAGGACGG CTCCTACTTC 
CGAGACCTGT GGAACATCCT GGACTTCGTG GTGGTGGTTG GCGCATTGGT GGCCTTTGCT 
CTGGCGAATG CTTTGGGAAC TAACAAGGGC CGGGATATTA AGACCATCAA GTCCCTGAGG 
GTTCTCCGTG TCCTGAGGCC GCTGAAGACC ATCAAGCGCC TGCCCAAGCT CAAGGCTGTC 
TTTGACTGTG TGGTCACCTC CTTGAAAAAC GTCTTCAACA TACTCATTGT CTACAAGCTC 
TTCATGTTCA TCTTTGCTGT CATCGCAGTT CAGCTTTTCA AGGGCAAGTT CTTCTACTGC 
ACGGACAGTT CCAAGGACAC AGAGAAGGAG TGCATAGGCA ACTATGTAGA TCACGAGAAA 
AACAAGATGG AGGTGAAAGG CCGAGAGTGG AAGCGCCATG AATTCCATTA TGACAACATC 
ATCTGGGCTC TGCTGACCCT CTTCACTGTC TCCACAGGGG AAGGGTGGCC TCAGGTCTTG 
CAGCACTCGG TCGATGTGAC AGAGGAAGAC CGAGGCCCAA GCCGCAGCAA CCGGATGGAG 
ATGTCCATCT TTTATGTGGT CTACTTTGTG GTCTTCCCCT TCTTCTTTGT CAACATCTTT 
GTGGCTCTCA TCATCATCAC CTTCCAGGAG CAAGGGGACA AGATGATGGA AGAGTGCAGC 
CTGGAGAAGA ATGAGAGGGC ATGCATCGAC TTCGCCATCA GCGCCAAACC GCTCACCCGC 
TACATGCCCC AGAACAGGCA CACCTTCCAG TACCGCGTCT GGCACTTCGT GGTGTCTCCA 
TCCTTTGAGT ACACCATCAT GGCCATGATC GCCCTGAACA CCGTCGTGCT GATGATGAAG 
TACTACTCTG CTCCCTGTAC CTATGAGCTG GCCCTGAAGT ACCTAAATAT CGCCTTCACC 
ATGGTGTTTT CCCTGGAATG TGTCCTGAAG ATCATTGCAT TTGGCTTCTT GAACTATTTC 
CGAGACACCT GGAATATCTT TGACTTCATC ACTGTGATTG GCAGTATCAC AGAAATTATT 
CTGACGGACA GCAAGCTGGT GAACACTAGT GGCTTCAACA TGAGCTTTCT GAAGCTCTTC 
CGAGCGGCCC GCCTCATCAA GCTGCTGCGT CAGGGCTACA CCATCCGCAT TTTACTGTGG 
ACCTTCGTGC AGTCCTTTAA GGCCCTTCCC TATGTCTGCC TTCTGATCGC CATGCTTTTC 
TTCATCTATG CCATCATTGG GATGCAGGTC TTTGGAAACA TAAAGTTAGA TGAGGAGAGT 
CACATCAACC GGCACAACAA CTTCCGGAGT TTCTTTGGGT CCCTCATGCT GCTCTTCAGG 
AGTGCCACGG GTGAGGCCTG GCAGGAGATA ATGCTGTCGT GTCTCGGGGA GAAGGGCTGT 
GAGCCCGACA CCACTGCGCC GTCGGGGCAG AGCGAGAGCG AGCGCTGTGG CACCGACCTG 
GCCTACGTGT ACTTCGTCTC CTTCATCTTC TTCTGCTCCT TCCTGATGCT CAACCTGTTC 
GTGGCTGTCA TCATGGACAA CTTTGAGTAC CTGACTCGGG ACTCGTCCAT CCTGGGGCCT 
CACCATCTGG ACGAGTTTGT CCGAGTCTGG GCAGAGTATG ACAGAGCAGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1E ( NM_001191123.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001178052.1 Bos taurus calcium voltage-gated channel subunit alpha1 E (CACNA1E), mRNA.

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CloneID	OBa216174
Clone ID Related Accession (Same CDS sequence)	NM_001191123.2
Accession Version	NM_001191123.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6807bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-22
Organism	Bos taurus(cattle)
Product	voltage-dependent R-type calcium channel subunit alpha-1E
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02043534.1, DAAA02043538.1 and DAAA02043541.1. On Jun 29, 2017 this sequence version replaced NM_001191123.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02822092, SAMN02822093 [ECO:0000348] ##Evidence-Data-END##

ATGGCTCGCT TCGGGGAGGC GGTGGTCGGC AGGCCGGGGT CGGGCGATGG AGACTCGGAC 
CAGAGCAGGA ACCGGCAAGG AACCCCCGTG CCGGCCTCCG GGCCGGCGGC CGCCTACAAG 
CAGTCGAAAG CGCAGAGGGC GCGGACTATG GCTTTGTACA ACCCTATTCC CGTCCGGCAG 
AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA 
TACGCCAAGA AGCTCATCGA TTGGCCGCCG TTTGAGTACA TGATCTTGGC CACCATCATT 
GCCAATTGCA TCGTCTTGGC CCTGGAGCAA CATCTTCCTG AGGATGACAA GACCCCAATG 
TCCCGCAGAC TGGAGAAGAC AGAACCCTAT TTCATTGGGA TCTTTTGCTT TGAAGCTGGG 
ATCAAAATCG TGGCCCTGGG GTTCATCTTC CATAAGGGAT CATACCTCCG CAATGGTTGG 
AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CTACTGCGGG AACCCACTTC 
AACACCCATG TGGACCTGAG GACCCTCCGG GCCGTGCGTG TCCTGAGGCC CCTGAAGCTC 
GTGTCGGGGA TACCTAGCCT ACAGATTGTG TTGAAATCCA TCATGAAGGC CATGGTGCCT 
CTCCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATCCTGA TGTTTGCTAT CATCGGCTTG 
GAGTTCTACA GTGGAAAGTT GCACCGAGCG TGCTTCATGA ACAATTCAGG TATTCTAGAA 
GGATTTGACC CCCCTCACCC ATGTGGCGTG CAGGGCTGCC CAGCTGGTTA TGAATGCCGG 
GACTGGATCG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCCGTGCTG 
ACCGTCTTCC AGTGTATCAC CATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT 
GCCTTAGGAG CCACCTGGAA TTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC 
TTTGTTCTCA ACCTTGTCCT GGGAGTGCTT TCTGGGGAAT TTGCCAAAGA GAGAGAGAGA 
GTGGAGAACC GGAGAGCCTT CATGAAACTG CGGCGCCAGC AGCAGATCGA GCGTGAGCTT 
AACGGGTACC GCGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTTGCTGA AGAAAACAAA 
AATGCTGGAA CGTCAGCCTT AGAAGTGCTT CGGAGGGCAA CCATCAAAAG GAGCCGGACC 
GAGGCCATGG CACGGGACTC CAGCGATGAG CACTGCGTGG ACATCTCCTC TGTGGGCACA 
CCTCTCGCCC GAGCCAGCAT CAAGAGCGCC AAGGTGGACG GGGCCTCCTA TTTCCGGCAC 
AAGGAGCGAC TTTTGCGGAT CTCTATCCGC CACATGGTTA AGTCCCAGGT GTTCTACTGG 
ATCGTGCTGA GCCTGGTGGC TCTCAACACC GCCTGTGTGG CCATCGTCCA CCACAACCAG 
CCCCAGTGGC TCACCCACCT CCTCTACTAT GCAGAGTTTC TGTTTCTGGG GCTCTTCCTT 
CTAGAGATGT CCCTGAAGAT GTACGGCATG GGGCCTCGCC TTTATTTTCA CTCTTCCTTC 
AACTGCTTTG ACTTTGGGGT CACAGTGGGC AGCATCTTTG AAGTAGTCTG GGCCATCTTC 
AGACCTGGTA CATCTTTCGG AATCAGTGTC CTGCGAGCTC TCCGGCTTCT AAGAATATTT 
AAAATAACCA AGTATTGGGC ATCCCTCCGG AATTTGGTGG TCTCCTTGAT GAGTTCCATG 
AAGTCTATCA TCAGTTTACT CTTCCTCCTT TTCCTCTTCA TTGTTGTCTT TGCTCTGCTA 
GGAATGCAGC TGTTTGGGGG CAGGTTCAAC TTTAATGATG GGACTCCTTC CGCAAACTTT 
GACACCTTCC CTGCAGCCAT CATGACTGTA TTCCAGATCC TGACGGGTGA GGACTGGAAT 
GAGGTGATGT ACAACGGGAT CCGCTCCCAG GGTGGGGTCA GCTCGGGCAT GTGGTCTGCG 
ATCTATTTCA TTGTGCTTAC CTTGTTTGGA AACTACACAC TGCTGAATGT GTTCTTGGCC 
ATCGCTGTGG ACAATCTGGC CAACGCCCAG GAGCTGACCA AGGATGAACA AGAAGAAGAG 
GAGGCCTTCA ACCAGAAGCA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA 
CCCAACATGC CTTCCATCGA AAGAGACAGA AGGAGAAGAC ACCACATGTC GATGTGGGAG 
CCACGCAGCA GCCACCTGAG GGAGCGGAGG CGCCGGCATC ACATGTCCGT GTGGGAGCAG 
CGAACCAGCC AGTTGAGGAG GCACATGCAG ATGTCCAGCC AGGAGGCCCT CAACAAGGAG 
GAGGCCCCGC CCATGAACCC CCTCAACCCC CTCAACCCGC TGAGCCCCCT CAACCCGCTC 
AGCGCGCACC CCAGCCTCTA CCGGAGACCC CGGGCCATCG AGGGGCTAGC CCTGGGCCTG 
GGGCTGGAGA AGTGTGAGGA AGAGCGCGTC AGCCGTGGGG GGTCCCTCAA AGGGGATGGA 
GGGGACTTGG CGAGCGCCCT GGACAGCCAG AGGAGCCCCC TGGCCCTGGG CAAACGGGAG 
CCACCCTGGC TGGCCCGGCC CTGTCACGGG AACTGTGACC CGACCCAGCC GGAGGCGGGC 
AGTGGGGAGA CGGTGGTGAC CTTCGGGGAC CGGGCCCGGC ACAGGCAGAG CCAGCGGCGC 
AGCCGGCACC GCCGCGTCAG GACCGAGGGC AGGGAGCCCT CCTCCACCTC CAGAAGCAGG 
TCTGCCAGCC AGGAGCGGAG TCTGGACGAA GCTGTGCCCA GCGAGGGGGA GAGGGACCGT 
GAGCCCAGGG CTGGCCATGG GGGCAAAGAA CCCATGATCC AGGAGGAAGA GCGAGAGCGA 
TGCCAGGACT TACGGAGGAC CAACAGTCTG ATGGTGCCCA GAGGCTCCGG GCTGGCAGGA 
GCCCTGGATG AGGCCGACAC ACCCCTTGTC CCACCCCAGC CGGAGCTGGA AGTGGGGAAG 
GACGCAGCCC TGACGGAGCA GGAGCCGGAG GGCAGCAGTG AGCAGGCTCT GCTGGGGGAC 
GTGAAGCTGG ACATAGGCCG GGTCAGCCAG AGCGAGCCCG ACCTCTCCTG CGTCACGGCC 
AACACGGACA AGGCCACCAC CGAGAGCACC AGCGTCACTG TCACCATCCC CGACGTGGGC 
CCCTTGGTGG ACTCAACCGT GGTGCACATC AGCAACAAGA CTGATGGAGA AGCCAGTCCC 
TTGAAAGAGG CAGAGATCAG AGAGGATGAG GAGGAGGTGG AGAAGAAGCA GCAGAAGAAG 
GAGAAGCGTG AGACGGGCAA GGCCATGGTG CCCCACAGCT CGATGTTCAT CTTCAGCACC 
ACCAACCCGA TCCGCAGGGC CTGCCACTAC ATCGTGAACC TGCGCTACTT CGAGATGTGC 
ATCCTCCTGG TGATCGCAGC CAGCAGCATC GCCCTGGCGG CCGAGGACCC CGTCCTGACC 
AACTCAGAGC GCAACAAAGT GCTGAGGTAT TTTGACTATG TGTTCACTGG CGTGTTCACC 
TTTGAGATGG TTATAAAGAT GATAGACCAG GGCTTGATTC TGCAGGACGG CTCCTACTTC 
CGAGACCTGT GGAACATCCT GGACTTCGTG GTGGTGGTTG GCGCATTGGT GGCCTTTGCT 
CTGGCGAATG CTTTGGGAAC TAACAAGGGC CGGGATATTA AGACCATCAA GTCCCTGAGG 
GTTCTCCGTG TCCTGAGGCC GCTGAAGACC ATCAAGCGCC TGCCCAAGCT CAAGGCTGTC 
TTTGACTGTG TGGTCACCTC CTTGAAAAAC GTCTTCAACA TACTCATTGT CTACAAGCTC 
TTCATGTTCA TCTTTGCTGT CATCGCAGTT CAGCTTTTCA AGGGCAAGTT CTTCTACTGC 
ACGGACAGTT CCAAGGACAC AGAGAAGGAG TGCATAGGCA ACTATGTAGA TCACGAGAAA 
AACAAGATGG AGGTGAAAGG CCGAGAGTGG AAGCGCCATG AATTCCATTA TGACAACATC 
ATCTGGGCTC TGCTGACCCT CTTCACTGTC TCCACAGGGG AAGGGTGGCC TCAGGTCTTG 
CAGCACTCGG TCGATGTGAC AGAGGAAGAC CGAGGCCCAA GCCGCAGCAA CCGGATGGAG 
ATGTCCATCT TTTATGTGGT CTACTTTGTG GTCTTCCCCT TCTTCTTTGT CAACATCTTT 
GTGGCTCTCA TCATCATCAC CTTCCAGGAG CAAGGGGACA AGATGATGGA AGAGTGCAGC 
CTGGAGAAGA ATGAGAGGGC ATGCATCGAC TTCGCCATCA GCGCCAAACC GCTCACCCGC 
TACATGCCCC AGAACAGGCA CACCTTCCAG TACCGCGTCT GGCACTTCGT GGTGTCTCCA 
TCCTTTGAGT ACACCATCAT GGCCATGATC GCCCTGAACA CCGTCGTGCT GATGATGAAG 
TACTACTCTG CTCCCTGTAC CTATGAGCTG GCCCTGAAGT ACCTAAATAT CGCCTTCACC 
ATGGTGTTTT CCCTGGAATG TGTCCTGAAG ATCATTGCAT TTGGCTTCTT GAACTATTTC 
CGAGACACCT GGAATATCTT TGACTTCATC ACTGTGATTG GCAGTATCAC AGAAATTATT 
CTGACGGACA GCAAGCTGGT GAACACTAGT GGCTTCAACA TGAGCTTTCT GAAGCTCTTC 
CGAGCGGCCC GCCTCATCAA GCTGCTGCGT CAGGGCTACA CCATCCGCAT TTTACTGTGG 
ACCTTCGTGC AGTCCTTTAA GGCCCTTCCC TATGTCTGCC TTCTGATCGC CATGCTTTTC 
TTCATCTATG CCATCATTGG GATGCAGGTC TTTGGAAACA TAAAGTTAGA TGAGGAGAGT 
CACATCAACC GGCACAACAA CTTCCGGAGT TTCTTTGGGT CCCTCATGCT GCTCTTCAGG 
AGTGCCACGG GTGAGGCCTG GCAGGAGATA ATGCTGTCGT GTCTCGGGGA GAAGGGCTGT 
GAGCCCGACA CCACTGCGCC GTCGGGGCAG AGCGAGAGCG AGCGCTGTGG CACCGACCTG 
GCCTACGTGT ACTTCGTCTC CTTCATCTTC TTCTGCTCCT TCCTGATGCT CAACCTGTTC 
GTGGCTGTCA TCATGGACAA CTTTGAGTAC CTGACTCGGG ACTCGTCCAT CCTGGGGCCT 
CACCATCTGG ACGAGTTTGT CCGAGTCTGG GCAGAGTATG ACAGAGCAGC ATGTGGCCGC 
ATCCATTACA CTGAGATGTA TGAAATGCTG ACTCTCATGT CACCGCCGCT AGGCCTCGGC 
AAGAGATGTC CCTCCAAGGT GGCATATAAG AGGTTGGTCT TGATGAACAT GCCAGTGGCC 
GAGGACATGA CCGTCCACTT CACTTCCACG CTGATGGCTC TAATCCGGAC AGCTCTGGAC 
ATTAAAATCG CCAAAGGCGG TGCAGACAGG CAGCAGCTAG ACTCAGAGCT CCAGAAGGAG 
ACCCTGGCCA TCTGGCCTCA CCTGTCCCAG AAGATGCTGG ATCTGCTCGT GCCCATGCCC 
AAAGCCTCTG ACCTGACTGT GGGCAAAATC TATGCAGCAA TGATGATCAT GGACTACTAC 
AAACAGAGCA AGGTGAAGAA GCAGAGGCAG CAGCTGGAGG AACAGAAAAA CGCACCCATG 
TTCCAGCGCA TGGAGCCCTC GTCTCTGCCT CAGGAGATCA TCGCTAATGC CAAAGCCCTG 
CCTTATCTCC AGCAGGACCC CGTTCCAGGC CTGAGTGGCC GGAGTGCATA CCCTTCGATG 
AGTCCACTCT CCCCGCAGGA AATATTCCAG TTGGCCTGTA TGGACCCAGC TGATGATGGA 
CAGTTCCAGG AACGACAATC TCTGGTGGTG ACAGACCCCA GCTCCATGAG ACGTTCATTT 
TCCACCATTC GCGATAAGCG TTCAAATTCC TCGTGGCTGG AGGAATTCTC CATGGAGCGA 
AGCAGCGAAA ACACCTACAA GTCCCGGCGC CGGAGTTACC ACTCCTCCCT GAGGCTGTCA 
GCCCACCGTC TGAACTCGGA CTCAGGCCAC AAGTCCGACA CCCACCGCTC AGGGGGCAGA 
GAGCGGGGAC GATCGAAAGA GCGAAAGCAT CTTCTCTCTC CTGACGTCTC CCGCTGCAAC 
TCCGAGGAGC GAGGGACCCA GGCCGACTGG GAGTCCCCAG AGCGCCATCA GTCCCGGTCT 
CCCAGTGAAG GCAGGTCACA GACCCCCAAC AGACAGGGCA CGGGGTCCCT GAGTGAGAGC 
TCCATCCCCT CTGTCTCCGA CTCCAGCACC CCACGACGAA GTCGGCGGCA GCTCCCCCCA 
GTCCCTCCCA AGCCTCGGCC CCTCCTTTCC TACAGCTCTC TGATCAGACA CGCTGGGAGC 
ATCTCCCCAC CAGCTGATGG GAGCGAGGGT GGCTCCCCAC TGACCTCCCA AGCTCTGGAG 
AGCAACGACG CCGGCCTGCC CGAGTCTTCC AACCCCCGGC AGGGCCACCC CTCCCCACAG 
CGCTACATCT CCGAGCCCTA CCTGGCCCTG CACGAAGACT CCCACGCCTC GGACTGCGGC 
GAGGAGGAGA CGCTCACCTT TGAAGCAGCC GTGGCCACCA GCCTGGGCCG GTCCAACACC 
ATCGGCTCGG CCCCGCCCCT GCGGCACAGC TGGCAGATGC CCAATGGGCA CTATCGGCGG 
CGGAGGCGCG GGGGGCCTGG ACCGGGCACG ACGTGCGGAG CCGTCAGCGA CCTCCTGAGT 
GACACGGAAG AAGACGACAA ATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001178052.1
CDS	1..6807
Translation	MARFGEAVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECRDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNAGTSALEVLRRATIKRSRTEAMARDSSDEHCVDISSVGTPLARASIKSAKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSLVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLSAHPSLYRRPRAIEGLALGLGLEKCEEERVSRGGSLKGDGGDLASALDSQRSPLALGKREPPWLARPCHGNCDPTQPEAGSGETVVTFGDRARHRQSQRRSRHRRVRTEGREPSSTSRSRSASQERSLDEAVPSEGERDREPRAGHGGKEPMIQEEERERCQDLRRTNSLMVPRGSGLAGALDEADTPLVPPQPELEVGKDAALTEQEPEGSSEQALLGDVKLDIGRVSQSEPDLSCVTANTDKATTESTSVTVTIPDVGPLVDSTVVHISNKTDGEASPLKEAEIREDEEEVEKKQQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKIIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQSESERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVPGLSGRSAYPSMSPLSPQEIFQLACMDPADDGQFQERQSLVVTDPSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERHQSRSPSEGRSQTPNRQGTGSLSESSIPSVSDSSTPRRSRRQLPPVPPKPRPLLSYSSLIRHAGSISPPADGSEGGSPLTSQALESNDAGLPESSNPRQGHPSPQRYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGTTCGAVSDLLSDTEEDDKC

Target ORF information:

RefSeq Version	NM_001191123.2
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 E (CACNA1E), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001191123.2

ORF Insert Sequence:

ATGGCTCGCT TCGGGGAGGC GGTGGTCGGC AGGCCGGGGT CGGGCGATGG AGACTCGGAC 
CAGAGCAGGA ACCGGCAAGG AACCCCCGTG CCGGCCTCCG GGCCGGCGGC CGCCTACAAG 
CAGTCGAAAG CGCAGAGGGC GCGGACTATG GCTTTGTACA ACCCTATTCC CGTCCGGCAG 
AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA 
TACGCCAAGA AGCTCATCGA TTGGCCGCCG TTTGAGTACA TGATCTTGGC CACCATCATT 
GCCAATTGCA TCGTCTTGGC CCTGGAGCAA CATCTTCCTG AGGATGACAA GACCCCAATG 
TCCCGCAGAC TGGAGAAGAC AGAACCCTAT TTCATTGGGA TCTTTTGCTT TGAAGCTGGG 
ATCAAAATCG TGGCCCTGGG GTTCATCTTC CATAAGGGAT CATACCTCCG CAATGGTTGG 
AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CTACTGCGGG AACCCACTTC 
AACACCCATG TGGACCTGAG GACCCTCCGG GCCGTGCGTG TCCTGAGGCC CCTGAAGCTC 
GTGTCGGGGA TACCTAGCCT ACAGATTGTG TTGAAATCCA TCATGAAGGC CATGGTGCCT 
CTCCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATCCTGA TGTTTGCTAT CATCGGCTTG 
GAGTTCTACA GTGGAAAGTT GCACCGAGCG TGCTTCATGA ACAATTCAGG TATTCTAGAA 
GGATTTGACC CCCCTCACCC ATGTGGCGTG CAGGGCTGCC CAGCTGGTTA TGAATGCCGG 
GACTGGATCG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCCGTGCTG 
ACCGTCTTCC AGTGTATCAC CATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT 
GCCTTAGGAG CCACCTGGAA TTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC 
TTTGTTCTCA ACCTTGTCCT GGGAGTGCTT TCTGGGGAAT TTGCCAAAGA GAGAGAGAGA 
GTGGAGAACC GGAGAGCCTT CATGAAACTG CGGCGCCAGC AGCAGATCGA GCGTGAGCTT 
AACGGGTACC GCGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTTGCTGA AGAAAACAAA 
AATGCTGGAA CGTCAGCCTT AGAAGTGCTT CGGAGGGCAA CCATCAAAAG GAGCCGGACC 
GAGGCCATGG CACGGGACTC CAGCGATGAG CACTGCGTGG ACATCTCCTC TGTGGGCACA 
CCTCTCGCCC GAGCCAGCAT CAAGAGCGCC AAGGTGGACG GGGCCTCCTA TTTCCGGCAC 
AAGGAGCGAC TTTTGCGGAT CTCTATCCGC CACATGGTTA AGTCCCAGGT GTTCTACTGG 
ATCGTGCTGA GCCTGGTGGC TCTCAACACC GCCTGTGTGG CCATCGTCCA CCACAACCAG 
CCCCAGTGGC TCACCCACCT CCTCTACTAT GCAGAGTTTC TGTTTCTGGG GCTCTTCCTT 
CTAGAGATGT CCCTGAAGAT GTACGGCATG GGGCCTCGCC TTTATTTTCA CTCTTCCTTC 
AACTGCTTTG ACTTTGGGGT CACAGTGGGC AGCATCTTTG AAGTAGTCTG GGCCATCTTC 
AGACCTGGTA CATCTTTCGG AATCAGTGTC CTGCGAGCTC TCCGGCTTCT AAGAATATTT 
AAAATAACCA AGTATTGGGC ATCCCTCCGG AATTTGGTGG TCTCCTTGAT GAGTTCCATG 
AAGTCTATCA TCAGTTTACT CTTCCTCCTT TTCCTCTTCA TTGTTGTCTT TGCTCTGCTA 
GGAATGCAGC TGTTTGGGGG CAGGTTCAAC TTTAATGATG GGACTCCTTC CGCAAACTTT 
GACACCTTCC CTGCAGCCAT CATGACTGTA TTCCAGATCC TGACGGGTGA GGACTGGAAT 
GAGGTGATGT ACAACGGGAT CCGCTCCCAG GGTGGGGTCA GCTCGGGCAT GTGGTCTGCG 
ATCTATTTCA TTGTGCTTAC CTTGTTTGGA AACTACACAC TGCTGAATGT GTTCTTGGCC 
ATCGCTGTGG ACAATCTGGC CAACGCCCAG GAGCTGACCA AGGATGAACA AGAAGAAGAG 
GAGGCCTTCA ACCAGAAGCA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA 
CCCAACATGC CTTCCATCGA AAGAGACAGA AGGAGAAGAC ACCACATGTC GATGTGGGAG 
CCACGCAGCA GCCACCTGAG GGAGCGGAGG CGCCGGCATC ACATGTCCGT GTGGGAGCAG 
CGAACCAGCC AGTTGAGGAG GCACATGCAG ATGTCCAGCC AGGAGGCCCT CAACAAGGAG 
GAGGCCCCGC CCATGAACCC CCTCAACCCC CTCAACCCGC TGAGCCCCCT CAACCCGCTC 
AGCGCGCACC CCAGCCTCTA CCGGAGACCC CGGGCCATCG AGGGGCTAGC CCTGGGCCTG 
GGGCTGGAGA AGTGTGAGGA AGAGCGCGTC AGCCGTGGGG GGTCCCTCAA AGGGGATGGA 
GGGGACTTGG CGAGCGCCCT GGACAGCCAG AGGAGCCCCC TGGCCCTGGG CAAACGGGAG 
CCACCCTGGC TGGCCCGGCC CTGTCACGGG AACTGTGACC CGACCCAGCC GGAGGCGGGC 
AGTGGGGAGA CGGTGGTGAC CTTCGGGGAC CGGGCCCGGC ACAGGCAGAG CCAGCGGCGC 
AGCCGGCACC GCCGCGTCAG GACCGAGGGC AGGGAGCCCT CCTCCACCTC CAGAAGCAGG 
TCTGCCAGCC AGGAGCGGAG TCTGGACGAA GCTGTGCCCA GCGAGGGGGA GAGGGACCGT 
GAGCCCAGGG CTGGCCATGG GGGCAAAGAA CCCATGATCC AGGAGGAAGA GCGAGAGCGA 
TGCCAGGACT TACGGAGGAC CAACAGTCTG ATGGTGCCCA GAGGCTCCGG GCTGGCAGGA 
GCCCTGGATG AGGCCGACAC ACCCCTTGTC CCACCCCAGC CGGAGCTGGA AGTGGGGAAG 
GACGCAGCCC TGACGGAGCA GGAGCCGGAG GGCAGCAGTG AGCAGGCTCT GCTGGGGGAC 
GTGAAGCTGG ACATAGGCCG GGTCAGCCAG AGCGAGCCCG ACCTCTCCTG CGTCACGGCC 
AACACGGACA AGGCCACCAC CGAGAGCACC AGCGTCACTG TCACCATCCC CGACGTGGGC 
CCCTTGGTGG ACTCAACCGT GGTGCACATC AGCAACAAGA CTGATGGAGA AGCCAGTCCC 
TTGAAAGAGG CAGAGATCAG AGAGGATGAG GAGGAGGTGG AGAAGAAGCA GCAGAAGAAG 
GAGAAGCGTG AGACGGGCAA GGCCATGGTG CCCCACAGCT CGATGTTCAT CTTCAGCACC 
ACCAACCCGA TCCGCAGGGC CTGCCACTAC ATCGTGAACC TGCGCTACTT CGAGATGTGC 
ATCCTCCTGG TGATCGCAGC CAGCAGCATC GCCCTGGCGG CCGAGGACCC CGTCCTGACC 
AACTCAGAGC GCAACAAAGT GCTGAGGTAT TTTGACTATG TGTTCACTGG CGTGTTCACC 
TTTGAGATGG TTATAAAGAT GATAGACCAG GGCTTGATTC TGCAGGACGG CTCCTACTTC 
CGAGACCTGT GGAACATCCT GGACTTCGTG GTGGTGGTTG GCGCATTGGT GGCCTTTGCT 
CTGGCGAATG CTTTGGGAAC TAACAAGGGC CGGGATATTA AGACCATCAA GTCCCTGAGG 
GTTCTCCGTG TCCTGAGGCC GCTGAAGACC ATCAAGCGCC TGCCCAAGCT CAAGGCTGTC 
TTTGACTGTG TGGTCACCTC CTTGAAAAAC GTCTTCAACA TACTCATTGT CTACAAGCTC 
TTCATGTTCA TCTTTGCTGT CATCGCAGTT CAGCTTTTCA AGGGCAAGTT CTTCTACTGC 
ACGGACAGTT CCAAGGACAC AGAGAAGGAG TGCATAGGCA ACTATGTAGA TCACGAGAAA 
AACAAGATGG AGGTGAAAGG CCGAGAGTGG AAGCGCCATG AATTCCATTA TGACAACATC 
ATCTGGGCTC TGCTGACCCT CTTCACTGTC TCCACAGGGG AAGGGTGGCC TCAGGTCTTG 
CAGCACTCGG TCGATGTGAC AGAGGAAGAC CGAGGCCCAA GCCGCAGCAA CCGGATGGAG 
ATGTCCATCT TTTATGTGGT CTACTTTGTG GTCTTCCCCT TCTTCTTTGT CAACATCTTT 
GTGGCTCTCA TCATCATCAC CTTCCAGGAG CAAGGGGACA AGATGATGGA AGAGTGCAGC 
CTGGAGAAGA ATGAGAGGGC ATGCATCGAC TTCGCCATCA GCGCCAAACC GCTCACCCGC 
TACATGCCCC AGAACAGGCA CACCTTCCAG TACCGCGTCT GGCACTTCGT GGTGTCTCCA 
TCCTTTGAGT ACACCATCAT GGCCATGATC GCCCTGAACA CCGTCGTGCT GATGATGAAG 
TACTACTCTG CTCCCTGTAC CTATGAGCTG GCCCTGAAGT ACCTAAATAT CGCCTTCACC 
ATGGTGTTTT CCCTGGAATG TGTCCTGAAG ATCATTGCAT TTGGCTTCTT GAACTATTTC 
CGAGACACCT GGAATATCTT TGACTTCATC ACTGTGATTG GCAGTATCAC AGAAATTATT 
CTGACGGACA GCAAGCTGGT GAACACTAGT GGCTTCAACA TGAGCTTTCT GAAGCTCTTC 
CGAGCGGCCC GCCTCATCAA GCTGCTGCGT CAGGGCTACA CCATCCGCAT TTTACTGTGG 
ACCTTCGTGC AGTCCTTTAA GGCCCTTCCC TATGTCTGCC TTCTGATCGC CATGCTTTTC 
TTCATCTATG CCATCATTGG GATGCAGGTC TTTGGAAACA TAAAGTTAGA TGAGGAGAGT 
CACATCAACC GGCACAACAA CTTCCGGAGT TTCTTTGGGT CCCTCATGCT GCTCTTCAGG 
AGTGCCACGG GTGAGGCCTG GCAGGAGATA ATGCTGTCGT GTCTCGGGGA GAAGGGCTGT 
GAGCCCGACA CCACTGCGCC GTCGGGGCAG AGCGAGAGCG AGCGCTGTGG CACCGACCTG 
GCCTACGTGT ACTTCGTCTC CTTCATCTTC TTCTGCTCCT TCCTGATGCT CAACCTGTTC 
GTGGCTGTCA TCATGGACAA CTTTGAGTAC CTGACTCGGG ACTCGTCCAT CCTGGGGCCT 
CACCATCTGG ACGAGTTTGT CCGAGTCTGG GCAGAGTATG ACAGAGCAGC ATGTGGCCGC 
ATCCATTACA CTGAGATGTA TGAAATGCTG ACTCTCATGT CACCGCCGCT AGGCCTCGGC 
AAGAGATGTC CCTCCAAGGT GGCATATAAG AGGTTGGTCT TGATGAACAT GCCAGTGGCC 
GAGGACATGA CCGTCCACTT CACTTCCACG CTGATGGCTC TAATCCGGAC AGCTCTGGAC 
ATTAAAATCG CCAAAGGCGG TGCAGACAGG CAGCAGCTAG ACTCAGAGCT CCAGAAGGAG 
ACCCTGGCCA TCTGGCCTCA CCTGTCCCAG AAGATGCTGG ATCTGCTCGT GCCCATGCCC 
AAAGCCTCTG ACCTGACTGT GGGCAAAATC TATGCAGCAA TGATGATCAT GGACTACTAC 
AAACAGAGCA AGGTGAAGAA GCAGAGGCAG CAGCTGGAGG AACAGAAAAA CGCACCCATG 
TTCCAGCGCA TGGAGCCCTC GTCTCTGCCT CAGGAGATCA TCGCTAATGC CAAAGCCCTG 
CCTTATCTCC AGCAGGACCC CGTTCCAGGC CTGAGTGGCC GGAGTGCATA CCCTTCGATG 
AGTCCACTCT CCCCGCAGGA AATATTCCAG TTGGCCTGTA TGGACCCAGC TGATGATGGA 
CAGTTCCAGG AACGACAATC TCTGGTGGTG ACAGACCCCA GCTCCATGAG ACGTTCATTT 
TCCACCATTC GCGATAAGCG TTCAAATTCC TCGTGGCTGG AGGAATTCTC CATGGAGCGA 
AGCAGCGAAA ACACCTACAA GTCCCGGCGC CGGAGTTACC ACTCCTCCCT GAGGCTGTCA 
GCCCACCGTC TGAACTCGGA CTCAGGCCAC AAGTCCGACA CCCACCGCTC AGGGGGCAGA 
GAGCGGGGAC GATCGAAAGA GCGAAAGCAT CTTCTCTCTC CTGACGTCTC CCGCTGCAAC 
TCCGAGGAGC GAGGGACCCA GGCCGACTGG GAGTCCCCAG AGCGCCATCA GTCCCGGTCT 
CCCAGTGAAG GCAGGTCACA GACCCCCAAC AGACAGGGCA CGGGGTCCCT GAGTGAGAGC 
TCCATCCCCT CTGTCTCCGA CTCCAGCACC CCACGACGAA GTCGGCGGCA GCTCCCCCCA 
GTCCCTCCCA AGCCTCGGCC CCTCCTTTCC TACAGCTCTC TGATCAGACA CGCTGGGAGC 
ATCTCCCCAC CAGCTGATGG GAGCGAGGGT GGCTCCCCAC TGACCTCCCA AGCTCTGGAG 
AGCAACGACG CCGGCCTGCC CGAGTCTTCC AACCCCCGGC AGGGCCACCC CTCCCCACAG 
CGCTACATCT CCGAGCCCTA CCTGGCCCTG CACGAAGACT CCCACGCCTC GGACTGCGGC 
GAGGAGGAGA CGCTCACCTT TGAAGCAGCC GTGGCCACCA GCCTGGGCCG GTCCAACACC 
ATCGGCTCGG CCCCGCCCCT GCGGCACAGC TGGCAGATGC CCAATGGGCA CTATCGGCGG 
CGGAGGCGCG GGGGGCCTGG ACCGGGCACG ACGTGCGGAG CCGTCAGCGA CCTCCTGAGT 
GACACGGAAG AAGACGACAA ATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1E ( NM_001191123.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001178052.1 Bos taurus calcium voltage-gated channel subunit alpha1 E (CACNA1E), mRNA.

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CloneID	OBa238960
Clone ID Related Accession (Same CDS sequence)	NM_001191123.3
Accession Version	NM_001191123.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6807bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-28
Organism	Bos taurus(cattle)
Product	voltage-dependent R-type calcium channel subunit alpha-1E
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000016.1. On May 29, 2018 this sequence version replaced NM_001191123.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145414, SAMN03145415 [ECO:0000350] ##Evidence-Data-END##

ATGGCTCGCT TCGGGGAGGC GGTGGTCGGC AGGCCGGGGT CGGGCGATGG AGACTCGGAC 
CAGAGCAGGA ACCGGCAAGG AACCCCCGTG CCGGCCTCCG GGCCGGCGGC CGCCTACAAG 
CAGTCGAAAG CGCAGAGGGC GCGGACTATG GCTTTGTACA ACCCTATTCC CGTCCGGCAG 
AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA 
TACGCCAAGA AGCTCATCGA TTGGCCGCCG TTTGAGTACA TGATCTTGGC CACCATCATT 
GCCAATTGCA TCGTCTTGGC CCTGGAGCAA CATCTTCCTG AGGATGACAA GACCCCAATG 
TCCCGCAGAC TGGAGAAGAC AGAACCCTAT TTCATTGGGA TCTTTTGCTT TGAAGCTGGG 
ATCAAAATCG TGGCCCTGGG GTTCATCTTC CATAAGGGAT CATACCTCCG CAATGGTTGG 
AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CTACTGCGGG AACCCACTTC 
AACACCCATG TGGACCTGAG GACCCTCCGG GCCGTGCGTG TCCTGAGGCC CCTGAAGCTC 
GTGTCGGGGA TACCTAGCCT ACAGATTGTG TTGAAATCCA TCATGAAGGC CATGGTGCCT 
CTCCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATCCTGA TGTTTGCTAT CATCGGCTTG 
GAGTTCTACA GTGGAAAGTT GCACCGAGCG TGCTTCATGA ACAATTCAGG TATTCTAGAA 
GGATTTGACC CCCCTCACCC ATGTGGCGTG CAGGGCTGCC CAGCTGGTTA TGAATGCCGG 
GACTGGATCG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCCGTGCTG 
ACCGTCTTCC AGTGTATCAC CATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT 
GCCTTAGGAG CCACCTGGAA TTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC 
TTTGTTCTCA ACCTTGTCCT GGGAGTGCTT TCCGGGGAAT TTGCCAAAGA GAGAGAGAGA 
GTGGAGAACC GGAGAGCCTT CATGAAACTG CGGCGCCAGC AGCAGATCGA GCGTGAGCTT 
AACGGGTACC GCGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTTGCTGA AGAAAACAAA 
AATGCTGGAA CGTCAGCCTT AGAAGTGCTT CGGAGGGCAA CCATCAAAAG GAGCCGGACC 
GAGGCCATGG CACGGGACTC CAGCGATGAG CACTGCGTGG ACATCTCCTC TGTGGGCACA 
CCTCTCGCCC GAGCCAGCAT CAAGAGCGCC AAGGTGGACG GGGCCTCCTA TTTCCGGCAC 
AAGGAGCGAC TTTTGCGGAT CTCTATCCGC CACATGGTTA AGTCCCAGGT GTTCTACTGG 
ATCGTGCTGA GCCTGGTGGC TCTCAACACC GCCTGTGTGG CCATCGTCCA CCACAACCAG 
CCCCAGTGGC TCACCCACCT CCTCTACTAT GCAGAGTTTC TGTTTCTGGG GCTCTTCCTT 
CTAGAGATGT CCCTGAAGAT GTACGGCATG GGGCCTCGCC TTTATTTTCA CTCTTCCTTC 
AACTGCTTTG ACTTTGGGGT CACAGTGGGC AGCATCTTTG AAGTAGTCTG GGCCATCTTC 
AGACCTGGTA CATCTTTCGG AATCAGTGTC CTGCGAGCTC TCCGGCTTCT AAGAATATTT 
AAAATAACCA AGTATTGGGC ATCCCTCCGG AATTTGGTGG TCTCCTTGAT GAGTTCCATG 
AAGTCTATCA TCAGTTTACT CTTCCTCCTT TTCCTCTTCA TTGTTGTCTT TGCTCTGCTA 
GGAATGCAGC TGTTTGGGGG CAGGTTCAAC TTTAATGATG GGACTCCTTC CGCAAACTTT 
GACACCTTCC CTGCAGCCAT CATGACTGTA TTCCAGATCC TGACGGGTGA GGACTGGAAT 
GAGGTGATGT ACAACGGGAT CCGCTCCCAG GGTGGGGTCA GCTCGGGCAT GTGGTCTGCG 
ATCTATTTCA TTGTGCTTAC CTTGTTTGGA AACTACACAC TGCTGAATGT GTTCTTGGCC 
ATCGCTGTGG ACAATCTGGC CAACGCCCAG GAGCTGACCA AGGATGAACA AGAAGAAGAG 
GAGGCCTTCA ACCAGAAGCA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA 
CCCAACATGC CTTCCATCGA AAGAGACAGA AGGAGAAGAC ACCACATGTC GATGTGGGAG 
CCACGCAGCA GCCACCTGAG GGAGCGGAGG CGCCGGCATC ACATGTCCGT GTGGGAGCAG 
CGAACCAGCC AGTTGAGGAG GCACATGCAG ATGTCCAGCC AGGAGGCCCT CAACAAGGAG 
GAGGCCCCGC CCATGAACCC CCTCAACCCC CTCAACCCGC TGAGCCCCCT CAACCCGCTC 
AGCGCGCACC CCAGCCTCTA CCGGAGACCC CGGGCCATCG AGGGGCTAGC CCTGGGCCTG 
GGGCTGGAGA AGTGTGAGGA AGAGCGCGTC AGCCGTGGGG GGTCCCTCAA AGGGGATGGA 
GGGGACTTGG CGAGCGCCCT GGACAGCCAG AGGAGCCCCC TGGCCCTGGG CAAACGGGAG 
CCACCCTGGC TGGCCCGGCC CTGTCACGGG AACTGTGACC CGACCCAGCC GGAGGCGGGC 
AGTGGGGAGA CGGTGGTGAC CTTCGGGGAC CGGGCCCGGC ACAGGCAGAG CCAGCGGCGC 
AGCCGGCACC GCCGCGTCAG GACCGAGGGC AGGGAGCCCT CCTCCACCTC CAGAAGCAGG 
TCTGCCAGCC AGGAGCGGAG TCTGGACGAA GCTGTGCCCA GCGAGGGGGA GAGGGACCGT 
GAGCCCAGGG CTGGCCATGG GGGCAAAGAA CCCATGATCC AGGAGGAAGA GCGAGAGCGA 
TGCCAGGACT TACGGAGGAC CAACAGTCTG ATGGTGCCCA GAGGCTCCGG GCTGGCAGGA 
GCCCTGGATG AGGCCGACAC ACCCCTTGTC CCACCCCAGC CGGAGCTGGA AGTGGGGAAG 
GACGCAGCCC TGACGGAGCA GGAGCCGGAG GGCAGCAGTG AGCAGGCTCT GCTGGGGGAC 
GTGAAGCTGG ACATAGGCCG GGTCAGCCAG AGCGAGCCCG ACCTCTCCTG CGTCACGGCC 
AACACGGACA AGGCCACCAC CGAGAGCACC AGCGTCACTG TCACCATCCC CGACGTGGGC 
CCCTTGGTGG ACTCAACCGT GGTGCACATC AGCAACAAGA CTGATGGAGA AGCCAGTCCC 
TTGAAAGAGG CAGAGATCAG AGAGGATGAG GAGGAGGTGG AGAAGAAGCA GCAGAAGAAG 
GAGAAGCGTG AGACGGGCAA GGCCATGGTG CCCCACAGCT CGATGTTCAT CTTCAGCACC 
ACCAACCCGA TCCGCAGGGC CTGCCACTAC ATCGTGAACC TGCGCTACTT CGAGATGTGC 
ATCCTCCTGG TGATCGCAGC CAGCAGCATC GCCCTGGCGG CCGAGGACCC CGTCCTGACC 
AACTCAGAGC GCAACAAAGT GCTGAGGTAT TTTGACTATG TGTTCACTGG CGTGTTCACC 
TTTGAGATGG TTATAAAGAT GATAGACCAG GGCTTGATTC TGCAGGACGG CTCCTACTTC 
CGAGACCTGT GGAACATCCT GGACTTCGTG GTGGTGGTTG GCGCATTGGT GGCCTTTGCT 
CTGGCGAATG CTTTGGGAAC TAACAAGGGC CGGGATATTA AGACCATCAA GTCCCTGAGG 
GTTCTCCGTG TCCTGAGGCC GCTGAAGACC ATCAAGCGCC TGCCCAAGCT CAAGGCTGTC 
TTTGACTGTG TGGTCACCTC CTTGAAAAAC GTCTTCAACA TACTCATTGT CTACAAGCTC 
TTCATGTTCA TCTTTGCTGT CATCGCAGTT CAGCTTTTCA AGGGCAAGTT CTTCTACTGC 
ACGGACAGTT CCAAGGACAC AGAGAAGGAG TGCATAGGCA ACTATGTAGA TCACGAGAAA 
AACAAGATGG AGGTGAAAGG CCGAGAGTGG AAGCGCCATG AATTCCATTA TGACAACATC 
ATCTGGGCTC TGCTGACCCT CTTCACTGTC TCCACAGGGG AAGGGTGGCC TCAGGTCTTG 
CAGCACTCGG TCGATGTGAC AGAGGAAGAC CGAGGCCCAA GCCGCAGCAA CCGGATGGAG 
ATGTCCATCT TTTATGTGGT CTACTTTGTG GTCTTCCCCT TCTTCTTTGT CAACATCTTT 
GTGGCTCTCA TCATCATCAC CTTCCAGGAG CAAGGGGACA AGATGATGGA AGAGTGCAGC 
CTGGAGAAGA ATGAGAGGGC ATGCATCGAC TTCGCCATCA GCGCCAAACC GCTCACCCGC 
TACATGCCCC AGAACAGGCA CACCTTCCAG TACCGCGTCT GGCACTTCGT GGTGTCTCCA 
TCCTTTGAGT ACACCATCAT GGCCATGATC GCCCTGAACA CCGTCGTGCT GATGATGAAG 
TACTACTCTG CTCCCTGTAC CTATGAGCTG GCCCTGAAGT ACCTAAATAT CGCCTTCACC 
ATGGTGTTTT CCCTGGAATG TGTCCTGAAG ATCATTGCAT TTGGCTTCTT GAACTATTTC 
CGAGACACCT GGAATATCTT TGACTTCATC ACTGTGATTG GCAGTATCAC AGAAATTATT 
CTGACGGACA GCAAGCTGGT GAACACTAGT GGCTTCAACA TGAGCTTTCT GAAGCTCTTC 
CGAGCGGCCC GCCTCATCAA GCTGCTGCGT CAGGGCTACA CCATCCGCAT TTTACTGTGG 
ACCTTCGTGC AGTCCTTTAA GGCCCTTCCC TATGTCTGCC TTCTGATCGC CATGCTTTTC 
TTCATCTATG CCATCATTGG GATGCAGGTC TTTGGAAACA TAAAGTTAGA TGAGGAGAGT 
CACATCAACC GGCACAACAA CTTCCGGAGT TTCTTTGGGT CCCTCATGCT GCTCTTCAGG 
AGTGCCACGG GTGAGGCCTG GCAGGAGATA ATGCTGTCGT GTCTCGGGGA GAAGGGCTGT 
GAGCCCGACA CCACTGCGCC GTCGGGGCAG AGCGAGAGCG AGCGCTGTGG CACCGACCTG 
GCCTACGTGT ACTTCGTCTC CTTCATCTTC TTCTGCTCCT TCCTGATGCT CAACCTGTTC 
GTGGCTGTCA TCATGGACAA CTTTGAGTAC CTGACTCGGG ACTCGTCCAT CCTGGGGCCT 
CACCATCTGG ACGAGTTTGT CCGAGTCTGG GCAGAGTATG ACAGAGCAGC ATGTGGCCGC 
ATCCATTACA CTGAGATGTA TGAAATGCTG ACTCTCATGT CACCGCCGCT AGGCCTCGGC 
AAGAGATGTC CCTCCAAGGT GGCATATAAG AGGTTGGTCT TGATGAACAT GCCAGTGGCC 
GAGGACATGA CCGTCCACTT CACTTCCACG CTGATGGCTC TAATCCGGAC AGCTCTGGAC 
ATTAAAATCG CCAAAGGCGG TGCAGACAGG CAGCAGCTAG ACTCAGAGCT CCAGAAGGAG 
ACCCTGGCCA TCTGGCCTCA CCTGTCCCAG AAGATGCTGG ATCTGCTCGT GCCCATGCCC 
AAAGCCTCTG ACCTGACTGT GGGCAAAATC TATGCAGCAA TGATGATCAT GGACTACTAC 
AAACAGAGCA AGGTGAAGAA GCAGAGGCAG CAGCTGGAGG AACAGAAAAA CGCACCCATG 
TTCCAGCGCA TGGAGCCCTC GTCTCTGCCT CAGGAGATCA TCGCTAATGC CAAAGCCCTG 
CCTTATCTCC AGCAGGACCC CGTTCCAGGC CTGAGTGGCC GGAGTGCATA CCCTTCGATG 
AGTCCACTCT CCCCGCAGGA AATATTCCAG TTGGCCTGTA TGGACCCAGC TGATGATGGA 
CAGTTCCAGG AACGACAATC TCTGGTGGTG ACAGACCCCA GCTCCATGAG ACGTTCATTT 
TCCACCATTC GCGATAAGCG TTCAAATTCC TCGTGGCTGG AGGAATTCTC CATGGAGCGA 
AGCAGCGAAA ACACCTACAA GTCCCGGCGC CGGAGTTACC ACTCCTCCCT GAGGCTGTCA 
GCCCACCGTC TGAACTCGGA CTCAGGCCAC AAGTCCGACA CCCACCGCTC AGGGGGCAGA 
GAGCGGGGAC GATCGAAAGA GCGAAAGCAT CTTCTCTCTC CTGACGTCTC CCGCTGCAAC 
TCCGAGGAGC GAGGGACCCA GGCCGACTGG GAGTCCCCAG AGCGCCATCA GTCCCGGTCT 
CCCAGTGAAG GCAGGTCACA GACCCCCAAC AGACAGGGCA CGGGGTCCCT GAGTGAGAGC 
TCCATCCCCT CTGTCTCCGA CTCCAGCACC CCACGACGAA GTCGGCGGCA GCTCCCCCCA 
GTCCCTCCCA AGCCTCGGCC CCTCCTTTCC TACAGCTCTC TGATCAGACA CGCTGGGAGC 
ATCTCCCCAC CAGCTGATGG GAGCGAGGGT GGCTCCCCAC TGACCTCCCA AGCTCTGGAG 
AGCAACGACG CCGGCCTGCC CGAGTCTTCC AACCCCCGGC AGGGCCACCC CTCCCCACAG 
CGCTACATCT CCGAGCCCTA CCTGGCCCTG CACGAAGACT CCCACGCCTC GGACTGCGGC 
GAGGAGGAGA CGCTCACCTT TGAAGCAGCC GTGGCCACCA GCCTGGGCCG GTCCAACACC 
ATCGGCTCGG CCCCGCCCCT GCGGCACAGC TGGCAGATGC CCAATGGGCA CTATCGGCGG 
CGGAGGCGCG GGGGGCCTGG ACCGGGCACG ACGTGCGGAG CCGTCAGCGA CCTCCTGAGT 
GACACGGAAG AAGACGACAA ATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001178052.1
CDS	1..6807
Translation	MARFGEAVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECRDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNAGTSALEVLRRATIKRSRTEAMARDSSDEHCVDISSVGTPLARASIKSAKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSLVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLSAHPSLYRRPRAIEGLALGLGLEKCEEERVSRGGSLKGDGGDLASALDSQRSPLALGKREPPWLARPCHGNCDPTQPEAGSGETVVTFGDRARHRQSQRRSRHRRVRTEGREPSSTSRSRSASQERSLDEAVPSEGERDREPRAGHGGKEPMIQEEERERCQDLRRTNSLMVPRGSGLAGALDEADTPLVPPQPELEVGKDAALTEQEPEGSSEQALLGDVKLDIGRVSQSEPDLSCVTANTDKATTESTSVTVTIPDVGPLVDSTVVHISNKTDGEASPLKEAEIREDEEEVEKKQQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKIIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQSESERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVPGLSGRSAYPSMSPLSPQEIFQLACMDPADDGQFQERQSLVVTDPSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERHQSRSPSEGRSQTPNRQGTGSLSESSIPSVSDSSTPRRSRRQLPPVPPKPRPLLSYSSLIRHAGSISPPADGSEGGSPLTSQALESNDAGLPESSNPRQGHPSPQRYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGTTCGAVSDLLSDTEEDDKC

Target ORF information:

RefSeq Version	NM_001191123.3
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 E (CACNA1E), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001191123.3

ORF Insert Sequence:

ATGGCTCGCT TCGGGGAGGC GGTGGTCGGC AGGCCGGGGT CGGGCGATGG AGACTCGGAC 
CAGAGCAGGA ACCGGCAAGG AACCCCCGTG CCGGCCTCCG GGCCGGCGGC CGCCTACAAG 
CAGTCGAAAG CGCAGAGGGC GCGGACTATG GCTTTGTACA ACCCTATTCC CGTCCGGCAG 
AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA 
TACGCCAAGA AGCTCATCGA TTGGCCGCCG TTTGAGTACA TGATCTTGGC CACCATCATT 
GCCAATTGCA TCGTCTTGGC CCTGGAGCAA CATCTTCCTG AGGATGACAA GACCCCAATG 
TCCCGCAGAC TGGAGAAGAC AGAACCCTAT TTCATTGGGA TCTTTTGCTT TGAAGCTGGG 
ATCAAAATCG TGGCCCTGGG GTTCATCTTC CATAAGGGAT CATACCTCCG CAATGGTTGG 
AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CTACTGCGGG AACCCACTTC 
AACACCCATG TGGACCTGAG GACCCTCCGG GCCGTGCGTG TCCTGAGGCC CCTGAAGCTC 
GTGTCGGGGA TACCTAGCCT ACAGATTGTG TTGAAATCCA TCATGAAGGC CATGGTGCCT 
CTCCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATCCTGA TGTTTGCTAT CATCGGCTTG 
GAGTTCTACA GTGGAAAGTT GCACCGAGCG TGCTTCATGA ACAATTCAGG TATTCTAGAA 
GGATTTGACC CCCCTCACCC ATGTGGCGTG CAGGGCTGCC CAGCTGGTTA TGAATGCCGG 
GACTGGATCG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCCGTGCTG 
ACCGTCTTCC AGTGTATCAC CATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT 
GCCTTAGGAG CCACCTGGAA TTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC 
TTTGTTCTCA ACCTTGTCCT GGGAGTGCTT TCCGGGGAAT TTGCCAAAGA GAGAGAGAGA 
GTGGAGAACC GGAGAGCCTT CATGAAACTG CGGCGCCAGC AGCAGATCGA GCGTGAGCTT 
AACGGGTACC GCGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTTGCTGA AGAAAACAAA 
AATGCTGGAA CGTCAGCCTT AGAAGTGCTT CGGAGGGCAA CCATCAAAAG GAGCCGGACC 
GAGGCCATGG CACGGGACTC CAGCGATGAG CACTGCGTGG ACATCTCCTC TGTGGGCACA 
CCTCTCGCCC GAGCCAGCAT CAAGAGCGCC AAGGTGGACG GGGCCTCCTA TTTCCGGCAC 
AAGGAGCGAC TTTTGCGGAT CTCTATCCGC CACATGGTTA AGTCCCAGGT GTTCTACTGG 
ATCGTGCTGA GCCTGGTGGC TCTCAACACC GCCTGTGTGG CCATCGTCCA CCACAACCAG 
CCCCAGTGGC TCACCCACCT CCTCTACTAT GCAGAGTTTC TGTTTCTGGG GCTCTTCCTT 
CTAGAGATGT CCCTGAAGAT GTACGGCATG GGGCCTCGCC TTTATTTTCA CTCTTCCTTC 
AACTGCTTTG ACTTTGGGGT CACAGTGGGC AGCATCTTTG AAGTAGTCTG GGCCATCTTC 
AGACCTGGTA CATCTTTCGG AATCAGTGTC CTGCGAGCTC TCCGGCTTCT AAGAATATTT 
AAAATAACCA AGTATTGGGC ATCCCTCCGG AATTTGGTGG TCTCCTTGAT GAGTTCCATG 
AAGTCTATCA TCAGTTTACT CTTCCTCCTT TTCCTCTTCA TTGTTGTCTT TGCTCTGCTA 
GGAATGCAGC TGTTTGGGGG CAGGTTCAAC TTTAATGATG GGACTCCTTC CGCAAACTTT 
GACACCTTCC CTGCAGCCAT CATGACTGTA TTCCAGATCC TGACGGGTGA GGACTGGAAT 
GAGGTGATGT ACAACGGGAT CCGCTCCCAG GGTGGGGTCA GCTCGGGCAT GTGGTCTGCG 
ATCTATTTCA TTGTGCTTAC CTTGTTTGGA AACTACACAC TGCTGAATGT GTTCTTGGCC 
ATCGCTGTGG ACAATCTGGC CAACGCCCAG GAGCTGACCA AGGATGAACA AGAAGAAGAG 
GAGGCCTTCA ACCAGAAGCA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA 
CCCAACATGC CTTCCATCGA AAGAGACAGA AGGAGAAGAC ACCACATGTC GATGTGGGAG 
CCACGCAGCA GCCACCTGAG GGAGCGGAGG CGCCGGCATC ACATGTCCGT GTGGGAGCAG 
CGAACCAGCC AGTTGAGGAG GCACATGCAG ATGTCCAGCC AGGAGGCCCT CAACAAGGAG 
GAGGCCCCGC CCATGAACCC CCTCAACCCC CTCAACCCGC TGAGCCCCCT CAACCCGCTC 
AGCGCGCACC CCAGCCTCTA CCGGAGACCC CGGGCCATCG AGGGGCTAGC CCTGGGCCTG 
GGGCTGGAGA AGTGTGAGGA AGAGCGCGTC AGCCGTGGGG GGTCCCTCAA AGGGGATGGA 
GGGGACTTGG CGAGCGCCCT GGACAGCCAG AGGAGCCCCC TGGCCCTGGG CAAACGGGAG 
CCACCCTGGC TGGCCCGGCC CTGTCACGGG AACTGTGACC CGACCCAGCC GGAGGCGGGC 
AGTGGGGAGA CGGTGGTGAC CTTCGGGGAC CGGGCCCGGC ACAGGCAGAG CCAGCGGCGC 
AGCCGGCACC GCCGCGTCAG GACCGAGGGC AGGGAGCCCT CCTCCACCTC CAGAAGCAGG 
TCTGCCAGCC AGGAGCGGAG TCTGGACGAA GCTGTGCCCA GCGAGGGGGA GAGGGACCGT 
GAGCCCAGGG CTGGCCATGG GGGCAAAGAA CCCATGATCC AGGAGGAAGA GCGAGAGCGA 
TGCCAGGACT TACGGAGGAC CAACAGTCTG ATGGTGCCCA GAGGCTCCGG GCTGGCAGGA 
GCCCTGGATG AGGCCGACAC ACCCCTTGTC CCACCCCAGC CGGAGCTGGA AGTGGGGAAG 
GACGCAGCCC TGACGGAGCA GGAGCCGGAG GGCAGCAGTG AGCAGGCTCT GCTGGGGGAC 
GTGAAGCTGG ACATAGGCCG GGTCAGCCAG AGCGAGCCCG ACCTCTCCTG CGTCACGGCC 
AACACGGACA AGGCCACCAC CGAGAGCACC AGCGTCACTG TCACCATCCC CGACGTGGGC 
CCCTTGGTGG ACTCAACCGT GGTGCACATC AGCAACAAGA CTGATGGAGA AGCCAGTCCC 
TTGAAAGAGG CAGAGATCAG AGAGGATGAG GAGGAGGTGG AGAAGAAGCA GCAGAAGAAG 
GAGAAGCGTG AGACGGGCAA GGCCATGGTG CCCCACAGCT CGATGTTCAT CTTCAGCACC 
ACCAACCCGA TCCGCAGGGC CTGCCACTAC ATCGTGAACC TGCGCTACTT CGAGATGTGC 
ATCCTCCTGG TGATCGCAGC CAGCAGCATC GCCCTGGCGG CCGAGGACCC CGTCCTGACC 
AACTCAGAGC GCAACAAAGT GCTGAGGTAT TTTGACTATG TGTTCACTGG CGTGTTCACC 
TTTGAGATGG TTATAAAGAT GATAGACCAG GGCTTGATTC TGCAGGACGG CTCCTACTTC 
CGAGACCTGT GGAACATCCT GGACTTCGTG GTGGTGGTTG GCGCATTGGT GGCCTTTGCT 
CTGGCGAATG CTTTGGGAAC TAACAAGGGC CGGGATATTA AGACCATCAA GTCCCTGAGG 
GTTCTCCGTG TCCTGAGGCC GCTGAAGACC ATCAAGCGCC TGCCCAAGCT CAAGGCTGTC 
TTTGACTGTG TGGTCACCTC CTTGAAAAAC GTCTTCAACA TACTCATTGT CTACAAGCTC 
TTCATGTTCA TCTTTGCTGT CATCGCAGTT CAGCTTTTCA AGGGCAAGTT CTTCTACTGC 
ACGGACAGTT CCAAGGACAC AGAGAAGGAG TGCATAGGCA ACTATGTAGA TCACGAGAAA 
AACAAGATGG AGGTGAAAGG CCGAGAGTGG AAGCGCCATG AATTCCATTA TGACAACATC 
ATCTGGGCTC TGCTGACCCT CTTCACTGTC TCCACAGGGG AAGGGTGGCC TCAGGTCTTG 
CAGCACTCGG TCGATGTGAC AGAGGAAGAC CGAGGCCCAA GCCGCAGCAA CCGGATGGAG 
ATGTCCATCT TTTATGTGGT CTACTTTGTG GTCTTCCCCT TCTTCTTTGT CAACATCTTT 
GTGGCTCTCA TCATCATCAC CTTCCAGGAG CAAGGGGACA AGATGATGGA AGAGTGCAGC 
CTGGAGAAGA ATGAGAGGGC ATGCATCGAC TTCGCCATCA GCGCCAAACC GCTCACCCGC 
TACATGCCCC AGAACAGGCA CACCTTCCAG TACCGCGTCT GGCACTTCGT GGTGTCTCCA 
TCCTTTGAGT ACACCATCAT GGCCATGATC GCCCTGAACA CCGTCGTGCT GATGATGAAG 
TACTACTCTG CTCCCTGTAC CTATGAGCTG GCCCTGAAGT ACCTAAATAT CGCCTTCACC 
ATGGTGTTTT CCCTGGAATG TGTCCTGAAG ATCATTGCAT TTGGCTTCTT GAACTATTTC 
CGAGACACCT GGAATATCTT TGACTTCATC ACTGTGATTG GCAGTATCAC AGAAATTATT 
CTGACGGACA GCAAGCTGGT GAACACTAGT GGCTTCAACA TGAGCTTTCT GAAGCTCTTC 
CGAGCGGCCC GCCTCATCAA GCTGCTGCGT CAGGGCTACA CCATCCGCAT TTTACTGTGG 
ACCTTCGTGC AGTCCTTTAA GGCCCTTCCC TATGTCTGCC TTCTGATCGC CATGCTTTTC 
TTCATCTATG CCATCATTGG GATGCAGGTC TTTGGAAACA TAAAGTTAGA TGAGGAGAGT 
CACATCAACC GGCACAACAA CTTCCGGAGT TTCTTTGGGT CCCTCATGCT GCTCTTCAGG 
AGTGCCACGG GTGAGGCCTG GCAGGAGATA ATGCTGTCGT GTCTCGGGGA GAAGGGCTGT 
GAGCCCGACA CCACTGCGCC GTCGGGGCAG AGCGAGAGCG AGCGCTGTGG CACCGACCTG 
GCCTACGTGT ACTTCGTCTC CTTCATCTTC TTCTGCTCCT TCCTGATGCT CAACCTGTTC 
GTGGCTGTCA TCATGGACAA CTTTGAGTAC CTGACTCGGG ACTCGTCCAT CCTGGGGCCT 
CACCATCTGG ACGAGTTTGT CCGAGTCTGG GCAGAGTATG ACAGAGCAGC ATGTGGCCGC 
ATCCATTACA CTGAGATGTA TGAAATGCTG ACTCTCATGT CACCGCCGCT AGGCCTCGGC 
AAGAGATGTC CCTCCAAGGT GGCATATAAG AGGTTGGTCT TGATGAACAT GCCAGTGGCC 
GAGGACATGA CCGTCCACTT CACTTCCACG CTGATGGCTC TAATCCGGAC AGCTCTGGAC 
ATTAAAATCG CCAAAGGCGG TGCAGACAGG CAGCAGCTAG ACTCAGAGCT CCAGAAGGAG 
ACCCTGGCCA TCTGGCCTCA CCTGTCCCAG AAGATGCTGG ATCTGCTCGT GCCCATGCCC 
AAAGCCTCTG ACCTGACTGT GGGCAAAATC TATGCAGCAA TGATGATCAT GGACTACTAC 
AAACAGAGCA AGGTGAAGAA GCAGAGGCAG CAGCTGGAGG AACAGAAAAA CGCACCCATG 
TTCCAGCGCA TGGAGCCCTC GTCTCTGCCT CAGGAGATCA TCGCTAATGC CAAAGCCCTG 
CCTTATCTCC AGCAGGACCC CGTTCCAGGC CTGAGTGGCC GGAGTGCATA CCCTTCGATG 
AGTCCACTCT CCCCGCAGGA AATATTCCAG TTGGCCTGTA TGGACCCAGC TGATGATGGA 
CAGTTCCAGG AACGACAATC TCTGGTGGTG ACAGACCCCA GCTCCATGAG ACGTTCATTT 
TCCACCATTC GCGATAAGCG TTCAAATTCC TCGTGGCTGG AGGAATTCTC CATGGAGCGA 
AGCAGCGAAA ACACCTACAA GTCCCGGCGC CGGAGTTACC ACTCCTCCCT GAGGCTGTCA 
GCCCACCGTC TGAACTCGGA CTCAGGCCAC AAGTCCGACA CCCACCGCTC AGGGGGCAGA 
GAGCGGGGAC GATCGAAAGA GCGAAAGCAT CTTCTCTCTC CTGACGTCTC CCGCTGCAAC 
TCCGAGGAGC GAGGGACCCA GGCCGACTGG GAGTCCCCAG AGCGCCATCA GTCCCGGTCT 
CCCAGTGAAG GCAGGTCACA GACCCCCAAC AGACAGGGCA CGGGGTCCCT GAGTGAGAGC 
TCCATCCCCT CTGTCTCCGA CTCCAGCACC CCACGACGAA GTCGGCGGCA GCTCCCCCCA 
GTCCCTCCCA AGCCTCGGCC CCTCCTTTCC TACAGCTCTC TGATCAGACA CGCTGGGAGC 
ATCTCCCCAC CAGCTGATGG GAGCGAGGGT GGCTCCCCAC TGACCTCCCA AGCTCTGGAG 
AGCAACGACG CCGGCCTGCC CGAGTCTTCC AACCCCCGGC AGGGCCACCC CTCCCCACAG 
CGCTACATCT CCGAGCCCTA CCTGGCCCTG CACGAAGACT CCCACGCCTC GGACTGCGGC 
GAGGAGGAGA CGCTCACCTT TGAAGCAGCC GTGGCCACCA GCCTGGGCCG GTCCAACACC 
ATCGGCTCGG CCCCGCCCCT GCGGCACAGC TGGCAGATGC CCAATGGGCA CTATCGGCGG 
CGGAGGCGCG GGGGGCCTGG ACCGGGCACG ACGTGCGGAG CCGTCAGCGA CCTCCTGAGT 
GACACGGAAG AAGACGACAA ATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1E ( XM_024976061.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024831829.1 Bos taurus calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X1, mRNA.

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CloneID	OBa223446
Clone ID Related Accession (Same CDS sequence)	XM_024976061.1
Accession Version	XM_024976061.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGGG TTGATGAAGT TTGTGTATGG ATGTACATAG CCATCTTGGA GGCTGTCCTT 
CTCTCTTTGG TGATTCTGTT TCTTAACTCC TCCTTTCCCA CGCCGTTTGA GTACATGATC 
TTGGCCACCA TCATTGCCAA TTGCATCGTC TTGGCCCTGG AGCAACATCT TCCTGAGGAT 
GACAAGACCC CAATGTCCCG CAGACTGGAG AAGACAGAAC CCTATTTCAT TGGGATCTTT 
TGCTTTGAAG CTGGGATCAA AATCGTGGCC CTGGGGTTCA TCTTCCATAA GGGATCATAC 
CTCCGCAATG GTTGGAATGT CATGGACTTC ATCGTGGTCC TCAGTGGCAT CCTGGCTACT 
GCGGGAACCC ACTTCAACAC CCATGTGGAC CTGAGGACCC TCCGGGCCGT GCGTGTCCTG 
AGGCCCCTGA AGCTCGTGTC GGGGATACCT AGCCTACAGA TTGTGTTGAA ATCCATCATG 
AAGGCCATGG TGCCTCTCCT GCAGATTGGC CTTCTGCTCT TCTTTGCCAT CCTGATGTTT 
GCTATCATCG GCTTGGAGTT CTACAGTGGA AAGTTGCACC GAGCGTGCTT CATGAACAAT 
TCAGGTATTC TAGAAGGATT TGACCCCCCT CACCCATGTG GCGTGCAGGG CTGCCCAGCT 
GGTTATGAAT GCCGGGACTG GATCGGCCCC AATGATGGGA TCACCCAGTT TGATAACATC 
CTTTTTGCCG TGCTGACCGT CTTCCAGTGT ATCACCATGG AAGGGTGGAC CACTGTGCTG 
TACAATACCA ATGATGCCTT AGGAGCCACC TGGAATTGGC TGTACTTCAT CCCCCTCATC 
ATCATTGGAT CCTTCTTTGT TCTCAACCTT GTCCTGGGAG TGCTTTCCGG GGAATTTGCC 
AAAGAGAGAG AGAGAGTGGA GAACCGGAGA GCCTTCATGA AACTGCGGCG CCAGCAGCAG 
ATCGAGCGTG AGCTTAACGG GTACCGCGCC TGGATAGACA AAGCAGAGGA AGTCATGCTT 
GCTGAAGAAA ACAAAAATGC TGGAACGTCA GCCTTAGAAG TGCTTCGGAG GGCAACCATC 
AAAAGGAGCC GGACCGAGGC CATGGCACGG GACTCCAGCG ATGAGCACTG CGTGGACATC 
TCCTCTGTGG GCACACCTCT CGCCCGAGCC AGCATCAAGA GCGCCAAGGT GGACGGGGCC 
TCCTATTTCC GGCACAAGGA GCGACTTTTG CGGATCTCTA TCCGCCACAT GGTTAAGTCC 
CAGGTGTTCT ACTGGATCGT GCTGAGCCTG GTGGCTCTCA ACACCGCCTG TGTGGCCATC 
GTCCACCACA ACCAGCCCCA GTGGCTCACC CACCTCCTCT ACTATGCAGA GTTTCTGTTT 
CTGGGGCTCT TCCTTCTAGA GATGTCCCTG AAGATGTACG GCATGGGGCC TCGCCTTTAT 
TTTCACTCTT CCTTCAACTG CTTTGACTTT GGGGTCACAG TGGGCAGCAT CTTTGAAGTA 
GTCTGGGCCA TCTTCAGACC TGGTACATCT TTCGGAATCA GTGTCCTGCG AGCTCTCCGG 
CTTCTAAGAA TATTTAAAAT AACCAAGTAT TGGGCATCCC TCCGGAATTT GGTGGTCTCC 
TTGATGAGTT CCATGAAGTC TATCATCAGT TTACTCTTCC TCCTTTTCCT CTTCATTGTT 
GTCTTTGCTC TGCTAGGAAT GCAGCTGTTT GGGGGCAGGT TCAACTTTAA TGATGGGACT 
CCTTCCGCAA ACTTTGACAC CTTCCCTGCA GCCATCATGA CTGTATTCCA GATCCTGACG 
GGTGAGGACT GGAATGAGGT GATGTACAAC GGGATCCGCT CCCAGGGTGG GGTCAGCTCG 
GGCATGTGGT CTGCGATCTA TTTCATTGTG CTTACCTTGT TTGGAAACTA CACACTGCTG 
AATGTGTTCT TGGCCATCGC TGTGGACAAT CTGGCCAACG CCCAGGAGCT GACCAAGGAT 
GAACAAGAAG AAGAGGAGGC CTTCAACCAG AAGCATGCAC TGCAGAAGGC CAAGGAGGTC 
AGCCCGATGT CTGCACCCAA CATGCCTTCC ATCGAGAGGG AGCGGAGGCG CCGGCATCAC 
ATGTCCGTGT GGGAGCAGCG AACCAGCCAG TTGAGGAGGC ACATGCAGAT GTCCAGCCAG 
GAGGCCCTCA ACAAGGAGGA GGCCCCGCCC ATGAACCCCC TCAACCCCCT CAACCCGCTG 
AGCCCCCTCA ACCCGCTCAG CGCGCACCCC AGCCTCTACC GGAGACCCCG GGCCATCGAG 
GGGCTAGCCC TGGGCCTGGG GCTGGAGAAG TGTGAGGAAG AGCGCGTCAG CCGTGGGGGG 
TCCCTCAAAG GGGATGGAGG GGACTTGGCG AGCGCCCTGG ACAGCCAGAG GAGCCCCCTG 
GCCCTGGGCA AACGGGAGCC ACCCTGGCTG GCCCGGCCCT GTCACGGGAA CTGTGACCCG 
ACCCAGCCGG AGGCGGGCAG TGGGGAGACG GTGGTGACCT TCGGGGACCG GGCCCGGCAC 
AGGCAGAGCC AGCGGCGCAG CCGGCACCGC CGCGTCAGGA CCGAGGGCAG GGAGCCCTCC 
TCCACCTCCA GAAGCAGGTC TGCCAGCCAG GAGCGGAGTC TGGACGAAGC TGTGCCCAGC 
GAGGGGGAGA GGGACCGTGA GCCCAGGGCT GGCCATGGGG GCAAAGAACC CATGATCCAG 
GAGGAAGAGC GAGAGCGATG CCAGGACTTA CGGAGGACCA ACAGTCTGAT GGTGCCCAGA 
GGCTCCGGGC TGGCAGGAGC CCTGGATGAG GCCGACACAC CCCTTGTCCC ACCCCAGCCG 
GAGCTGGAAG TGGGGAAGGA CGCAGCCCTG ACGGAGCAGG AGCCGGAGGG CAGCAGTGAG 
CAGGCTCTGC TGGGGGACGT GAAGCTGGAC ATAGGCCGGG TCAGCCAGAG CGAGCCCGAC 
CTCTCCTGCG TCACGGCCAA CACGGACAAG GCCACCACCG AGAGCACCAG CGTCACTGTC 
ACCATCCCCG ACGTGGGCCC CTTGGTGGAC TCAACCGTGG TGCACATCAG CAACAAGACT 
GATGGAGAAG CCAGTCCCTT GAAAGAGGCA GAGATCAGAG AGGATGAGGA GGAGGTGGAG 
AAGAAGCAGC AGAAGAAGGA GAAGCGTGAG ACGGGCAAGG CCATGGTGCC CCACAGCTCG 
ATGTTCATCT TCAGCACCAC CAACCCGATC CGCAGGGCCT GCCACTACAT CGTGAACCTG 
CGCTACTTCG AGATGTGCAT CCTCCTGGTG ATCGCAGCCA GCAGCATCGC CCTGGCGGCC 
GAGGACCCCG TCCTGACCAA CTCAGAGCGC AACAAAGTGC TGAGGTATTT TGACTATGTG 
TTCACTGGCG TGTTCACCTT TGAGATGGTT ATAAAGATGA TAGACCAGGG CTTGATTCTG 
CAGGACGGCT CCTACTTCCG AGACCTGTGG AACATCCTGG ACTTCGTGGT GGTGGTTGGC 
GCATTGGTGG CCTTTGCTCT GGCAACTAAC AAGGGCCGGG ATATTAAGAC CATCAAGTCC 
CTGAGGGTTC TCCGTGTCCT GAGGCCGCTG AAGACCATCA AGCGCCTGCC CAAGCTCAAG 
GCTGTCTTTG ACTGTGTGGT CACCTCCTTG AAAAACGTCT TCAACATACT CATTGTCTAC 
AAGCTCTTCA TGTTCATCTT TGCTGTCATC GCAGTTCAGC TTTTCAAGGG CAAGTTCTTC 
TACTGCACGG ACAGTTCCAA GGACACAGAG AAGGAGTGCA TAGGCAACTA TGTAGATCAC 
GAGAAAAACA AGATGGAGGT GAAAGGCCGA GAGTGGAAGC GCCATGAATT CCATTATGAC 
AACATCATCT GGGCTCTGCT GACCCTCTTC ACTGTCTCCA CAGGGGAAGG GTGGCCTCAG 
GTCTTGCAGC ACTCGGTCGA TGTGACAGAG GAAGACCGAG GCCCAAGCCG CAGCAACCGG 
ATGGAGATGT CCATCTTTTA TGTGGTCTAC TTTGTGGTCT TCCCCTTCTT CTTTGTCAAC 
ATCTTTGTGG CTCTCATCAT CATCACCTTC CAGGAGCAAG GGGACAAGAT GATGGAAGAG 
TGCAGCCTGG AGAAGAATGA GAGGGCATGC ATCGACTTCG CCATCAGCGC CAAACCGCTC 
ACCCGCTACA TGCCCCAGAA CAGGCACACC TTCCAGTACC GCGTCTGGCA CTTCGTGGTG 
TCTCCATCCT TTGAGTACAC CATCATGGCC ATGATCGCCC TGAACACCGT CGTGCTGATG 
ATGAAGTACT ACTCTGCTCC CTGTACCTAT GAGCTGGCCC TGAAGTACCT AAATATCGCC 
TTCACCATGG TGTTTTCCCT GGAATGTGTC CTGAAGATCA TTGCATTTGG CTTCTTGAAC 
TATTTCCGAG ACACCTGGAA TATCTTTGAC TTCATCACTG TGATTGGCAG TATCACAGAA 
ATTATTCTGA CGGACAGCAA GCTGGTGAAC ACTAGTGGCT TCAACATGAG CTTTCTGAAG 
CTCTTCCGAG CGGCCCGCCT CATCAAGCTG CTGCGTCAGG GCTACACCAT CCGCATTTTA 
CTGTGGACCT TCGTGCAGTC CTTTAAGGCC CTTCCCTATG TCTGCCTTCT GATCGCCATG 
CTTTTCTTCA TCTATGCCAT CATTGGGATG CAGGTCTTTG GAAACATAAA GTTAGATGAG 
GAGAGTCACA TCAACCGGCA CAACAACTTC CGGAGTTTCT TTGGGTCCCT CATGCTGCTC 
TTCAGGAGTG CCACGGGTGA GGCCTGGCAG GAGATAATGC TGTCGTGTCT CGGGGAGAAG 
GGCTGTGAGC CCGACACCAC TGCGCCGTCG GGGCAGAGCG AGAGCGAGCG CTGTGGCACC 
GACCTGGCCT ACGTGTACTT CGTCTCCTTC ATCTTCTTCT GCTCCTTCCT GATGCTCAAC 
CTGTTCGTGG CTGTCATCAT GGACAACTTT GAGTACCTGA CTCGGGACTC GTCCATCCTG 
GGGCCTCACC ATCTGGACGA GTTTGTCCGA GTCTGGGCAG AGTATGACAG AGCAGCATGT 
GGCCGCATCC ATTACACTGA GATGTATGAA ATGCTGACTC TCATGTCACC GCCGCTAGGC 
CTCGGCAAGA GATGTCCCTC CAAGGTGGCA TATAAGAGGT TGGTCTTGAT GAACATGCCA 
GTGGCCGAGG ACATGACCGT CCACTTCACT TCCACGCTGA TGGCTCTAAT CCGGACAGCT 
CTGGACATTA AAATCGCCAA AGGCGGTGCA GACAGGCAGC AGCTAGACTC AGAGCTCCAG 
AAGGAGACCC TGGCCATCTG GCCTCACCTG TCCCAGAAGA TGCTGGATCT GCTCGTGCCC 
ATGCCCAAAG CCTCTGACCT GACTGTGGGC AAAATCTATG CAGCAATGAT GATCATGGAC 
TACTACAAAC AGAGCAAGGT GAAGAAGCAG AGGCAGCAGC TGGAGGAACA GAAAAACGCA 
CCCATGTTCC AGCGCATGGA GCCCTCGTCT CTGCCTCAGG AGATCATCGC TAATGCCAAA 
GCCCTGCCTT ATCTCCAGCA GGACCCCGTT CCAGGCCTGA GTGGCCGGAG TGCATACCCT 
TCGATGAGTC CACTCTCCCC GCAGGAAATA TTCCAGTTGG CCTGTATGGA CCCAGCTGAT 
GATGGACAGT TCCAGGAACG ACAATCTCTG GAGCCAGAGG TTAGTGAATT AAAAAGCGTG 
CAGCCCTCTA ACCATGGCAT CTACCTTCCT TCGGACACCC AGGAGCATGC GGGCTCTGGG 
AAGGCATCCT CTATGCCACG CCTGACTGTG GATCCCCAGG TGGTGACAGA CCCCAGCTCC 
ATGAGACGTT CATTTTCCAC CATTCGCGAT AAGCGTTCAA ATTCCTCGTG GCTGGAGGAA 
TTCTCCATGG AGCGAAGCAG CGAAAACACC TACAAGTCCC GGCGCCGGAG TTACCACTCC 
TCCCTGAGGC TGTCAGCCCA CCGTCTGAAC TCGGACTCAG GCCACAAGTC CGACACCCAC 
CGCTCAGGGG GCAGAGAGCG GGGACGATCG AAAGAGCGAA AGCATCTTCT CTCTCCTGAC 
GTCTCCCGCT GCAACTCCGA GGAGCGAGGG ACCCAGGCCG ACTGGGAGTC CCCAGAGCGC 
CATCAGTCCC GGTCTCCCAG TGAAGGCAGG TCACAGACCC CCAACAGACA GGGCACGGGG 
TCCCTGAGTG AGAGCTCCAT CCCCTCTGTC TCCGACTCCA GCACCCCACG ACGAAGTCGG 
CGGCAGCTCC CCCCAGTCCC TCCCAAGCCT CGGCCCCTCC TTTCCTACAG CTCTCTGATC 
AGACACGCTG GGAGCATCTC CCCACCAGCT GATGGGAGCG AGGGTGGCTC CCCACTGACC 
TCCCAAGCTC TGGAGAGCAA CGACGCCGGC CTGCCCGAGT CTTCCAACCC CCGGCAGGGC 
CACCCCTCCC CACAGCGCTA CATCTCCGAG CCCTACCTGG CCCTGCACGA AGACTCCCAC 
GCCTCGGACT GCGGCGAGGA GGAGACGCTC ACCTTTGAAG CAGCCGTGGC CACCAGCCTG 
GGCCGGTCCA ACACCATCGG CTCGGCCCCG CCCCTGCGGC ACAGCTGGCA GATGCCCAAT 
GGGCACTATC GGCGGCGGAG GCGCGGGGGG CCTGGACCGG GCACGACGTG CGGAGCCGTC 
AGCGACCTCC TGAGTGACAC GGAAGAAGAC GACAAATGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024831829.1
CDS	7..6708
Translation	MMWVDEVCVWMYIAILEAVLLSLVILFLNSSFPTPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECRDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNAGTSALEVLRRATIKRSRTEAMARDSSDEHCVDISSVGTPLARASIKSAKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSLVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLSAHPSLYRRPRAIEGLALGLGLEKCEEERVSRGGSLKGDGGDLASALDSQRSPLALGKREPPWLARPCHGNCDPTQPEAGSGETVVTFGDRARHRQSQRRSRHRRVRTEGREPSSTSRSRSASQERSLDEAVPSEGERDREPRAGHGGKEPMIQEEERERCQDLRRTNSLMVPRGSGLAGALDEADTPLVPPQPELEVGKDAALTEQEPEGSSEQALLGDVKLDIGRVSQSEPDLSCVTANTDKATTESTSVTVTIPDVGPLVDSTVVHISNKTDGEASPLKEAEIREDEEEVEKKQQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALATNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKIIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQSESERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVPGLSGRSAYPSMSPLSPQEIFQLACMDPADDGQFQERQSLEPEVSELKSVQPSNHGIYLPSDTQEHAGSGKASSMPRLTVDPQVVTDPSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERHQSRSPSEGRSQTPNRQGTGSLSESSIPSVSDSSTPRRSRRQLPPVPPKPRPLLSYSSLIRHAGSISPPADGSEGGSPLTSQALESNDAGLPESSNPRQGHPSPQRYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGTTCGAVSDLLSDTEEDDKC

Target ORF information:

RefSeq Version	XM_024976061.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976061.1

ORF Insert Sequence:

ATGATGTGGG TTGATGAAGT TTGTGTATGG ATGTACATAG CCATCTTGGA GGCTGTCCTT 
CTCTCTTTGG TGATTCTGTT TCTTAACTCC TCCTTTCCCA CGCCGTTTGA GTACATGATC 
TTGGCCACCA TCATTGCCAA TTGCATCGTC TTGGCCCTGG AGCAACATCT TCCTGAGGAT 
GACAAGACCC CAATGTCCCG CAGACTGGAG AAGACAGAAC CCTATTTCAT TGGGATCTTT 
TGCTTTGAAG CTGGGATCAA AATCGTGGCC CTGGGGTTCA TCTTCCATAA GGGATCATAC 
CTCCGCAATG GTTGGAATGT CATGGACTTC ATCGTGGTCC TCAGTGGCAT CCTGGCTACT 
GCGGGAACCC ACTTCAACAC CCATGTGGAC CTGAGGACCC TCCGGGCCGT GCGTGTCCTG 
AGGCCCCTGA AGCTCGTGTC GGGGATACCT AGCCTACAGA TTGTGTTGAA ATCCATCATG 
AAGGCCATGG TGCCTCTCCT GCAGATTGGC CTTCTGCTCT TCTTTGCCAT CCTGATGTTT 
GCTATCATCG GCTTGGAGTT CTACAGTGGA AAGTTGCACC GAGCGTGCTT CATGAACAAT 
TCAGGTATTC TAGAAGGATT TGACCCCCCT CACCCATGTG GCGTGCAGGG CTGCCCAGCT 
GGTTATGAAT GCCGGGACTG GATCGGCCCC AATGATGGGA TCACCCAGTT TGATAACATC 
CTTTTTGCCG TGCTGACCGT CTTCCAGTGT ATCACCATGG AAGGGTGGAC CACTGTGCTG 
TACAATACCA ATGATGCCTT AGGAGCCACC TGGAATTGGC TGTACTTCAT CCCCCTCATC 
ATCATTGGAT CCTTCTTTGT TCTCAACCTT GTCCTGGGAG TGCTTTCCGG GGAATTTGCC 
AAAGAGAGAG AGAGAGTGGA GAACCGGAGA GCCTTCATGA AACTGCGGCG CCAGCAGCAG 
ATCGAGCGTG AGCTTAACGG GTACCGCGCC TGGATAGACA AAGCAGAGGA AGTCATGCTT 
GCTGAAGAAA ACAAAAATGC TGGAACGTCA GCCTTAGAAG TGCTTCGGAG GGCAACCATC 
AAAAGGAGCC GGACCGAGGC CATGGCACGG GACTCCAGCG ATGAGCACTG CGTGGACATC 
TCCTCTGTGG GCACACCTCT CGCCCGAGCC AGCATCAAGA GCGCCAAGGT GGACGGGGCC 
TCCTATTTCC GGCACAAGGA GCGACTTTTG CGGATCTCTA TCCGCCACAT GGTTAAGTCC 
CAGGTGTTCT ACTGGATCGT GCTGAGCCTG GTGGCTCTCA ACACCGCCTG TGTGGCCATC 
GTCCACCACA ACCAGCCCCA GTGGCTCACC CACCTCCTCT ACTATGCAGA GTTTCTGTTT 
CTGGGGCTCT TCCTTCTAGA GATGTCCCTG AAGATGTACG GCATGGGGCC TCGCCTTTAT 
TTTCACTCTT CCTTCAACTG CTTTGACTTT GGGGTCACAG TGGGCAGCAT CTTTGAAGTA 
GTCTGGGCCA TCTTCAGACC TGGTACATCT TTCGGAATCA GTGTCCTGCG AGCTCTCCGG 
CTTCTAAGAA TATTTAAAAT AACCAAGTAT TGGGCATCCC TCCGGAATTT GGTGGTCTCC 
TTGATGAGTT CCATGAAGTC TATCATCAGT TTACTCTTCC TCCTTTTCCT CTTCATTGTT 
GTCTTTGCTC TGCTAGGAAT GCAGCTGTTT GGGGGCAGGT TCAACTTTAA TGATGGGACT 
CCTTCCGCAA ACTTTGACAC CTTCCCTGCA GCCATCATGA CTGTATTCCA GATCCTGACG 
GGTGAGGACT GGAATGAGGT GATGTACAAC GGGATCCGCT CCCAGGGTGG GGTCAGCTCG 
GGCATGTGGT CTGCGATCTA TTTCATTGTG CTTACCTTGT TTGGAAACTA CACACTGCTG 
AATGTGTTCT TGGCCATCGC TGTGGACAAT CTGGCCAACG CCCAGGAGCT GACCAAGGAT 
GAACAAGAAG AAGAGGAGGC CTTCAACCAG AAGCATGCAC TGCAGAAGGC CAAGGAGGTC 
AGCCCGATGT CTGCACCCAA CATGCCTTCC ATCGAGAGGG AGCGGAGGCG CCGGCATCAC 
ATGTCCGTGT GGGAGCAGCG AACCAGCCAG TTGAGGAGGC ACATGCAGAT GTCCAGCCAG 
GAGGCCCTCA ACAAGGAGGA GGCCCCGCCC ATGAACCCCC TCAACCCCCT CAACCCGCTG 
AGCCCCCTCA ACCCGCTCAG CGCGCACCCC AGCCTCTACC GGAGACCCCG GGCCATCGAG 
GGGCTAGCCC TGGGCCTGGG GCTGGAGAAG TGTGAGGAAG AGCGCGTCAG CCGTGGGGGG 
TCCCTCAAAG GGGATGGAGG GGACTTGGCG AGCGCCCTGG ACAGCCAGAG GAGCCCCCTG 
GCCCTGGGCA AACGGGAGCC ACCCTGGCTG GCCCGGCCCT GTCACGGGAA CTGTGACCCG 
ACCCAGCCGG AGGCGGGCAG TGGGGAGACG GTGGTGACCT TCGGGGACCG GGCCCGGCAC 
AGGCAGAGCC AGCGGCGCAG CCGGCACCGC CGCGTCAGGA CCGAGGGCAG GGAGCCCTCC 
TCCACCTCCA GAAGCAGGTC TGCCAGCCAG GAGCGGAGTC TGGACGAAGC TGTGCCCAGC 
GAGGGGGAGA GGGACCGTGA GCCCAGGGCT GGCCATGGGG GCAAAGAACC CATGATCCAG 
GAGGAAGAGC GAGAGCGATG CCAGGACTTA CGGAGGACCA ACAGTCTGAT GGTGCCCAGA 
GGCTCCGGGC TGGCAGGAGC CCTGGATGAG GCCGACACAC CCCTTGTCCC ACCCCAGCCG 
GAGCTGGAAG TGGGGAAGGA CGCAGCCCTG ACGGAGCAGG AGCCGGAGGG CAGCAGTGAG 
CAGGCTCTGC TGGGGGACGT GAAGCTGGAC ATAGGCCGGG TCAGCCAGAG CGAGCCCGAC 
CTCTCCTGCG TCACGGCCAA CACGGACAAG GCCACCACCG AGAGCACCAG CGTCACTGTC 
ACCATCCCCG ACGTGGGCCC CTTGGTGGAC TCAACCGTGG TGCACATCAG CAACAAGACT 
GATGGAGAAG CCAGTCCCTT GAAAGAGGCA GAGATCAGAG AGGATGAGGA GGAGGTGGAG 
AAGAAGCAGC AGAAGAAGGA GAAGCGTGAG ACGGGCAAGG CCATGGTGCC CCACAGCTCG 
ATGTTCATCT TCAGCACCAC CAACCCGATC CGCAGGGCCT GCCACTACAT CGTGAACCTG 
CGCTACTTCG AGATGTGCAT CCTCCTGGTG ATCGCAGCCA GCAGCATCGC CCTGGCGGCC 
GAGGACCCCG TCCTGACCAA CTCAGAGCGC AACAAAGTGC TGAGGTATTT TGACTATGTG 
TTCACTGGCG TGTTCACCTT TGAGATGGTT ATAAAGATGA TAGACCAGGG CTTGATTCTG 
CAGGACGGCT CCTACTTCCG AGACCTGTGG AACATCCTGG ACTTCGTGGT GGTGGTTGGC 
GCATTGGTGG CCTTTGCTCT GGCAACTAAC AAGGGCCGGG ATATTAAGAC CATCAAGTCC 
CTGAGGGTTC TCCGTGTCCT GAGGCCGCTG AAGACCATCA AGCGCCTGCC CAAGCTCAAG 
GCTGTCTTTG ACTGTGTGGT CACCTCCTTG AAAAACGTCT TCAACATACT CATTGTCTAC 
AAGCTCTTCA TGTTCATCTT TGCTGTCATC GCAGTTCAGC TTTTCAAGGG CAAGTTCTTC 
TACTGCACGG ACAGTTCCAA GGACACAGAG AAGGAGTGCA TAGGCAACTA TGTAGATCAC 
GAGAAAAACA AGATGGAGGT GAAAGGCCGA GAGTGGAAGC GCCATGAATT CCATTATGAC 
AACATCATCT GGGCTCTGCT GACCCTCTTC ACTGTCTCCA CAGGGGAAGG GTGGCCTCAG 
GTCTTGCAGC ACTCGGTCGA TGTGACAGAG GAAGACCGAG GCCCAAGCCG CAGCAACCGG 
ATGGAGATGT CCATCTTTTA TGTGGTCTAC TTTGTGGTCT TCCCCTTCTT CTTTGTCAAC 
ATCTTTGTGG CTCTCATCAT CATCACCTTC CAGGAGCAAG GGGACAAGAT GATGGAAGAG 
TGCAGCCTGG AGAAGAATGA GAGGGCATGC ATCGACTTCG CCATCAGCGC CAAACCGCTC 
ACCCGCTACA TGCCCCAGAA CAGGCACACC TTCCAGTACC GCGTCTGGCA CTTCGTGGTG 
TCTCCATCCT TTGAGTACAC CATCATGGCC ATGATCGCCC TGAACACCGT CGTGCTGATG 
ATGAAGTACT ACTCTGCTCC CTGTACCTAT GAGCTGGCCC TGAAGTACCT AAATATCGCC 
TTCACCATGG TGTTTTCCCT GGAATGTGTC CTGAAGATCA TTGCATTTGG CTTCTTGAAC 
TATTTCCGAG ACACCTGGAA TATCTTTGAC TTCATCACTG TGATTGGCAG TATCACAGAA 
ATTATTCTGA CGGACAGCAA GCTGGTGAAC ACTAGTGGCT TCAACATGAG CTTTCTGAAG 
CTCTTCCGAG CGGCCCGCCT CATCAAGCTG CTGCGTCAGG GCTACACCAT CCGCATTTTA 
CTGTGGACCT TCGTGCAGTC CTTTAAGGCC CTTCCCTATG TCTGCCTTCT GATCGCCATG 
CTTTTCTTCA TCTATGCCAT CATTGGGATG CAGGTCTTTG GAAACATAAA GTTAGATGAG 
GAGAGTCACA TCAACCGGCA CAACAACTTC CGGAGTTTCT TTGGGTCCCT CATGCTGCTC 
TTCAGGAGTG CCACGGGTGA GGCCTGGCAG GAGATAATGC TGTCGTGTCT CGGGGAGAAG 
GGCTGTGAGC CCGACACCAC TGCGCCGTCG GGGCAGAGCG AGAGCGAGCG CTGTGGCACC 
GACCTGGCCT ACGTGTACTT CGTCTCCTTC ATCTTCTTCT GCTCCTTCCT GATGCTCAAC 
CTGTTCGTGG CTGTCATCAT GGACAACTTT GAGTACCTGA CTCGGGACTC GTCCATCCTG 
GGGCCTCACC ATCTGGACGA GTTTGTCCGA GTCTGGGCAG AGTATGACAG AGCAGCATGT 
GGCCGCATCC ATTACACTGA GATGTATGAA ATGCTGACTC TCATGTCACC GCCGCTAGGC 
CTCGGCAAGA GATGTCCCTC CAAGGTGGCA TATAAGAGGT TGGTCTTGAT GAACATGCCA 
GTGGCCGAGG ACATGACCGT CCACTTCACT TCCACGCTGA TGGCTCTAAT CCGGACAGCT 
CTGGACATTA AAATCGCCAA AGGCGGTGCA GACAGGCAGC AGCTAGACTC AGAGCTCCAG 
AAGGAGACCC TGGCCATCTG GCCTCACCTG TCCCAGAAGA TGCTGGATCT GCTCGTGCCC 
ATGCCCAAAG CCTCTGACCT GACTGTGGGC AAAATCTATG CAGCAATGAT GATCATGGAC 
TACTACAAAC AGAGCAAGGT GAAGAAGCAG AGGCAGCAGC TGGAGGAACA GAAAAACGCA 
CCCATGTTCC AGCGCATGGA GCCCTCGTCT CTGCCTCAGG AGATCATCGC TAATGCCAAA 
GCCCTGCCTT ATCTCCAGCA GGACCCCGTT CCAGGCCTGA GTGGCCGGAG TGCATACCCT 
TCGATGAGTC CACTCTCCCC GCAGGAAATA TTCCAGTTGG CCTGTATGGA CCCAGCTGAT 
GATGGACAGT TCCAGGAACG ACAATCTCTG GAGCCAGAGG TTAGTGAATT AAAAAGCGTG 
CAGCCCTCTA ACCATGGCAT CTACCTTCCT TCGGACACCC AGGAGCATGC GGGCTCTGGG 
AAGGCATCCT CTATGCCACG CCTGACTGTG GATCCCCAGG TGGTGACAGA CCCCAGCTCC 
ATGAGACGTT CATTTTCCAC CATTCGCGAT AAGCGTTCAA ATTCCTCGTG GCTGGAGGAA 
TTCTCCATGG AGCGAAGCAG CGAAAACACC TACAAGTCCC GGCGCCGGAG TTACCACTCC 
TCCCTGAGGC TGTCAGCCCA CCGTCTGAAC TCGGACTCAG GCCACAAGTC CGACACCCAC 
CGCTCAGGGG GCAGAGAGCG GGGACGATCG AAAGAGCGAA AGCATCTTCT CTCTCCTGAC 
GTCTCCCGCT GCAACTCCGA GGAGCGAGGG ACCCAGGCCG ACTGGGAGTC CCCAGAGCGC 
CATCAGTCCC GGTCTCCCAG TGAAGGCAGG TCACAGACCC CCAACAGACA GGGCACGGGG 
TCCCTGAGTG AGAGCTCCAT CCCCTCTGTC TCCGACTCCA GCACCCCACG ACGAAGTCGG 
CGGCAGCTCC CCCCAGTCCC TCCCAAGCCT CGGCCCCTCC TTTCCTACAG CTCTCTGATC 
AGACACGCTG GGAGCATCTC CCCACCAGCT GATGGGAGCG AGGGTGGCTC CCCACTGACC 
TCCCAAGCTC TGGAGAGCAA CGACGCCGGC CTGCCCGAGT CTTCCAACCC CCGGCAGGGC 
CACCCCTCCC CACAGCGCTA CATCTCCGAG CCCTACCTGG CCCTGCACGA AGACTCCCAC 
GCCTCGGACT GCGGCGAGGA GGAGACGCTC ACCTTTGAAG CAGCCGTGGC CACCAGCCTG 
GGCCGGTCCA ACACCATCGG CTCGGCCCCG CCCCTGCGGC ACAGCTGGCA GATGCCCAAT 
GGGCACTATC GGCGGCGGAG GCGCGGGGGG CCTGGACCGG GCACGACGTG CGGAGCCGTC 
AGCGACCTCC TGAGTGACAC GGAAGAAGAC GACAAATGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1E ( XM_015475247.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330733.1 Bos taurus calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X2, mRNA.

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CloneID	OBa223447
Clone ID Related Accession (Same CDS sequence)	XM_015475247.2
Accession Version	XM_015475247.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6924bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015475247.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGCT TCGGGGAGGC GGTGGTCGGC AGGCCGGGGT CGGGCGATGG AGACTCGGAC 
CAGAGCAGGA ACCGGCAAGG AACCCCCGTG CCGGCCTCCG GGCCGGCGGC CGCCTACAAG 
CAGTCGAAAG CGCAGAGGGC GCGGACTATG GCTTTGTACA ACCCTATTCC CGTCCGGCAG 
AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA 
TACGCCAAGA AGCTCATCGA TTGGCCGCCG TTTGAGTACA TGATCTTGGC CACCATCATT 
GCCAATTGCA TCGTCTTGGC CCTGGAGCAA CATCTTCCTG AGGATGACAA GACCCCAATG 
TCCCGCAGAC TGGAGAAGAC AGAACCCTAT TTCATTGGGA TCTTTTGCTT TGAAGCTGGG 
ATCAAAATCG TGGCCCTGGG GTTCATCTTC CATAAGGGAT CATACCTCCG CAATGGTTGG 
AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CTACTGCGGG AACCCACTTC 
AACACCCATG TGGACCTGAG GACCCTCCGG GCCGTGCGTG TCCTGAGGCC CCTGAAGCTC 
GTGTCGGGGA TACCTAGCCT ACAGATTGTG TTGAAATCCA TCATGAAGGC CATGGTGCCT 
CTCCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATCCTGA TGTTTGCTAT CATCGGCTTG 
GAGTTCTACA GTGGAAAGTT GCACCGAGCG TGCTTCATGA ACAATTCAGG TATTCTAGAA 
GGATTTGACC CCCCTCACCC ATGTGGCGTG CAGGGCTGCC CAGCTGGTTA TGAATGCCGG 
GACTGGATCG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCCGTGCTG 
ACCGTCTTCC AGTGTATCAC CATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT 
GCCTTAGGAG CCACCTGGAA TTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC 
TTTGTTCTCA ACCTTGTCCT GGGAGTGCTT TCCGGGGAAT TTGCCAAAGA GAGAGAGAGA 
GTGGAGAACC GGAGAGCCTT CATGAAACTG CGGCGCCAGC AGCAGATCGA GCGTGAGCTT 
AACGGGTACC GCGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTTGCTGA AGAAAACAAA 
AATGCTGGAA CGTCAGCCTT AGAAGTGCTT CGGAGGGCAA CCATCAAAAG GAGCCGGACC 
GAGGCCATGG CACGGGACTC CAGCGATGAG CACTGCGTGG ACATCTCCTC TGTGGGCACA 
CCTCTCGCCC GAGCCAGCAT CAAGAGCGCC AAGGTGGACG GGGCCTCCTA TTTCCGGCAC 
AAGGAGCGAC TTTTGCGGAT CTCTATCCGC CACATGGTTA AGTCCCAGGT GTTCTACTGG 
ATCGTGCTGA GCCTGGTGGC TCTCAACACC GCCTGTGTGG CCATCGTCCA CCACAACCAG 
CCCCAGTGGC TCACCCACCT CCTCTACTAT GCAGAGTTTC TGTTTCTGGG GCTCTTCCTT 
CTAGAGATGT CCCTGAAGAT GTACGGCATG GGGCCTCGCC TTTATTTTCA CTCTTCCTTC 
AACTGCTTTG ACTTTGGGGT CACAGTGGGC AGCATCTTTG AAGTAGTCTG GGCCATCTTC 
AGACCTGGTA CATCTTTCGG AATCAGTGTC CTGCGAGCTC TCCGGCTTCT AAGAATATTT 
AAAATAACCA AGTATTGGGC ATCCCTCCGG AATTTGGTGG TCTCCTTGAT GAGTTCCATG 
AAGTCTATCA TCAGTTTACT CTTCCTCCTT TTCCTCTTCA TTGTTGTCTT TGCTCTGCTA 
GGAATGCAGC TGTTTGGGGG CAGGTTCAAC TTTAATGATG GGACTCCTTC CGCAAACTTT 
GACACCTTCC CTGCAGCCAT CATGACTGTA TTCCAGATCC TGACGGGTGA GGACTGGAAT 
GAGGTGATGT ACAACGGGAT CCGCTCCCAG GGTGGGGTCA GCTCGGGCAT GTGGTCTGCG 
ATCTATTTCA TTGTGCTTAC CTTGTTTGGA AACTACACAC TGCTGAATGT GTTCTTGGCC 
ATCGCTGTGG ACAATCTGGC CAACGCCCAG GAGCTGACCA AGGATGAACA AGAAGAAGAG 
GAGGCCTTCA ACCAGAAGCA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA 
CCCAACATGC CTTCCATCGA AAGAGACAGA AGGAGAAGAC ACCACATGTC GATGTGGGAG 
CCACGCAGCA GCCACCTGAG GGAGCGGAGG CGCCGGCATC ACATGTCCGT GTGGGAGCAG 
CGAACCAGCC AGTTGAGGAG GCACATGCAG ATGTCCAGCC AGGAGGCCCT CAACAAGGAG 
GAGGCCCCGC CCATGAACCC CCTCAACCCC CTCAACCCGC TGAGCCCCCT CAACCCGCTC 
AGCGCGCACC CCAGCCTCTA CCGGAGACCC CGGGCCATCG AGGGGCTAGC CCTGGGCCTG 
GGGCTGGAGA AGTGTGAGGA AGAGCGCGTC AGCCGTGGGG GGTCCCTCAA AGGGGATGGA 
GGGGACTTGG CGAGCGCCCT GGACAGCCAG AGGAGCCCCC TGGCCCTGGG CAAACGGGAG 
CCACCCTGGC TGGCCCGGCC CTGTCACGGG AACTGTGACC CGACCCAGCC GGAGGCGGGC 
AGTGGGGAGA CGGTGGTGAC CTTCGGGGAC CGGGCCCGGC ACAGGCAGAG CCAGCGGCGC 
AGCCGGCACC GCCGCGTCAG GACCGAGGGC AGGGAGCCCT CCTCCACCTC CAGAAGCAGG 
TCTGCCAGCC AGGAGCGGAG TCTGGACGAA GCTGTGCCCA GCGAGGGGGA GAGGGACCGT 
GAGCCCAGGG CTGGCCATGG GGGCAAAGAA CCCATGATCC AGGAGGAAGA GCGAGAGCGA 
TGCCAGGACT TACGGAGGAC CAACAGTCTG ATGGTGCCCA GAGGCTCCGG GCTGGCAGGA 
GCCCTGGATG AGGCCGACAC ACCCCTTGTC CCACCCCAGC CGGAGCTGGA AGTGGGGAAG 
GACGCAGCCC TGACGGAGCA GGAGCCGGAG GGCAGCAGTG AGCAGGCTCT GCTGGGGGAC 
GTGAAGCTGG ACATAGGCCG GGTCAGCCAG AGCGAGCCCG ACCTCTCCTG CGTCACGGCC 
AACACGGACA AGGCCACCAC CGAGAGCACC AGCGTCACTG TCACCATCCC CGACGTGGGC 
CCCTTGGTGG ACTCAACCGT GGTGCACATC AGCAACAAGA CTGATGGAGA AGCCAGTCCC 
TTGAAAGAGG CAGAGATCAG AGAGGATGAG GAGGAGGTGG AGAAGAAGCA GCAGAAGAAG 
GAGAAGCGTG AGACGGGCAA GGCCATGGTG CCCCACAGCT CGATGTTCAT CTTCAGCACC 
ACCAACCCGA TCCGCAGGGC CTGCCACTAC ATCGTGAACC TGCGCTACTT CGAGATGTGC 
ATCCTCCTGG TGATCGCAGC CAGCAGCATC GCCCTGGCGG CCGAGGACCC CGTCCTGACC 
AACTCAGAGC GCAACAAAGT GCTGAGGTAT TTTGACTATG TGTTCACTGG CGTGTTCACC 
TTTGAGATGG TTATAAAGAT GATAGACCAG GGCTTGATTC TGCAGGACGG CTCCTACTTC 
CGAGACCTGT GGAACATCCT GGACTTCGTG GTGGTGGTTG GCGCATTGGT GGCCTTTGCT 
CTGGCAACTA ACAAGGGCCG GGATATTAAG ACCATCAAGT CCCTGAGGGT TCTCCGTGTC 
CTGAGGCCGC TGAAGACCAT CAAGCGCCTG CCCAAGCTCA AGGCTGTCTT TGACTGTGTG 
GTCACCTCCT TGAAAAACGT CTTCAACATA CTCATTGTCT ACAAGCTCTT CATGTTCATC 
TTTGCTGTCA TCGCAGTTCA GCTTTTCAAG GGCAAGTTCT TCTACTGCAC GGACAGTTCC 
AAGGACACAG AGAAGGAGTG CATAGGCAAC TATGTAGATC ACGAGAAAAA CAAGATGGAG 
GTGAAAGGCC GAGAGTGGAA GCGCCATGAA TTCCATTATG ACAACATCAT CTGGGCTCTG 
CTGACCCTCT TCACTGTCTC CACAGGGGAA GGGTGGCCTC AGGTCTTGCA GCACTCGGTC 
GATGTGACAG AGGAAGACCG AGGCCCAAGC CGCAGCAACC GGATGGAGAT GTCCATCTTT 
TATGTGGTCT ACTTTGTGGT CTTCCCCTTC TTCTTTGTCA ACATCTTTGT GGCTCTCATC 
ATCATCACCT TCCAGGAGCA AGGGGACAAG ATGATGGAAG AGTGCAGCCT GGAGAAGAAT 
GAGAGGGCAT GCATCGACTT CGCCATCAGC GCCAAACCGC TCACCCGCTA CATGCCCCAG 
AACAGGCACA CCTTCCAGTA CCGCGTCTGG CACTTCGTGG TGTCTCCATC CTTTGAGTAC 
ACCATCATGG CCATGATCGC CCTGAACACC GTCGTGCTGA TGATGAAGTA CTACTCTGCT 
CCCTGTACCT ATGAGCTGGC CCTGAAGTAC CTAAATATCG CCTTCACCAT GGTGTTTTCC 
CTGGAATGTG TCCTGAAGAT CATTGCATTT GGCTTCTTGA ACTATTTCCG AGACACCTGG 
AATATCTTTG ACTTCATCAC TGTGATTGGC AGTATCACAG AAATTATTCT GACGGACAGC 
AAGCTGGTGA ACACTAGTGG CTTCAACATG AGCTTTCTGA AGCTCTTCCG AGCGGCCCGC 
CTCATCAAGC TGCTGCGTCA GGGCTACACC ATCCGCATTT TACTGTGGAC CTTCGTGCAG 
TCCTTTAAGG CCCTTCCCTA TGTCTGCCTT CTGATCGCCA TGCTTTTCTT CATCTATGCC 
ATCATTGGGA TGCAGGTCTT TGGAAACATA AAGTTAGATG AGGAGAGTCA CATCAACCGG 
CACAACAACT TCCGGAGTTT CTTTGGGTCC CTCATGCTGC TCTTCAGGAG TGCCACGGGT 
GAGGCCTGGC AGGAGATAAT GCTGTCGTGT CTCGGGGAGA AGGGCTGTGA GCCCGACACC 
ACTGCGCCGT CGGGGCAGAG CGAGAGCGAG CGCTGTGGCA CCGACCTGGC CTACGTGTAC 
TTCGTCTCCT TCATCTTCTT CTGCTCCTTC CTGATGCTCA ACCTGTTCGT GGCTGTCATC 
ATGGACAACT TTGAGTACCT GACTCGGGAC TCGTCCATCC TGGGGCCTCA CCATCTGGAC 
GAGTTTGTCC GAGTCTGGGC AGAGTATGAC AGAGCAGCAT GTGGCCGCAT CCATTACACT 
GAGATGTATG AAATGCTGAC TCTCATGTCA CCGCCGCTAG GCCTCGGCAA GAGATGTCCC 
TCCAAGGTGG CATATAAGAG GTTGGTCTTG ATGAACATGC CAGTGGCCGA GGACATGACC 
GTCCACTTCA CTTCCACGCT GATGGCTCTA ATCCGGACAG CTCTGGACAT TAAAATCGCC 
AAAGGCGGTG CAGACAGGCA GCAGCTAGAC TCAGAGCTCC AGAAGGAGAC CCTGGCCATC 
TGGCCTCACC TGTCCCAGAA GATGCTGGAT CTGCTCGTGC CCATGCCCAA AGCCTCTGAC 
CTGACTGTGG GCAAAATCTA TGCAGCAATG ATGATCATGG ACTACTACAA ACAGAGCAAG 
GTGAAGAAGC AGAGGCAGCA GCTGGAGGAA CAGAAAAACG CACCCATGTT CCAGCGCATG 
GAGCCCTCGT CTCTGCCTCA GGAGATCATC GCTAATGCCA AAGCCCTGCC TTATCTCCAG 
CAGGACCCCG TTCCAGGCCT GAGTGGCCGG AGTGCATACC CTTCGATGAG TCCACTCTCC 
CCGCAGGAAA TATTCCAGTT GGCCTGTATG GACCCAGCTG ATGATGGACA GTTCCAGGAA 
CGACAATCTC TGGAGCCAGA GGTTAGTGAA TTAAAAAGCG TGCAGCCCTC TAACCATGGC 
ATCTACCTTC CTTCGGACAC CCAGGAGCAT GCGGGCTCTG GGAAGGCATC CTCTATGCCA 
CGCCTGACTG TGGATCCCCA GGTGGTGACA GACCCCAGCT CCATGAGACG TTCATTTTCC 
ACCATTCGCG ATAAGCGTTC AAATTCCTCG TGGCTGGAGG AATTCTCCAT GGAGCGAAGC 
AGCGAAAACA CCTACAAGTC CCGGCGCCGG AGTTACCACT CCTCCCTGAG GCTGTCAGCC 
CACCGTCTGA ACTCGGACTC AGGCCACAAG TCCGACACCC ACCGCTCAGG GGGCAGAGAG 
CGGGGACGAT CGAAAGAGCG AAAGCATCTT CTCTCTCCTG ACGTCTCCCG CTGCAACTCC 
GAGGAGCGAG GGACCCAGGC CGACTGGGAG TCCCCAGAGC GCCATCAGTC CCGGTCTCCC 
AGTGAAGGCA GGTCACAGAC CCCCAACAGA CAGGGCACGG GGTCCCTGAG TGAGAGCTCC 
ATCCCCTCTG TCTCCGACTC CAGCACCCCA CGACGAAGTC GGCGGCAGCT CCCCCCAGTC 
CCTCCCAAGC CTCGGCCCCT CCTTTCCTAC AGCTCTCTGA TCAGACACGC TGGGAGCATC 
TCCCCACCAG CTGATGGGAG CGAGGGTGGC TCCCCACTGA CCTCCCAAGC TCTGGAGAGC 
AACGACGCCG GCCTGCCCGA GTCTTCCAAC CCCCGGCAGG GCCACCCCTC CCCACAGCGC 
TACATCTCCG AGCCCTACCT GGCCCTGCAC GAAGACTCCC ACGCCTCGGA CTGCGGCGAG 
GAGGAGACGC TCACCTTTGA AGCAGCCGTG GCCACCAGCC TGGGCCGGTC CAACACCATC 
GGCTCGGCCC CGCCCCTGCG GCACAGCTGG CAGATGCCCA ATGGGCACTA TCGGCGGCGG 
AGGCGCGGGG GGCCTGGACC GGGCACGACG TGCGGAGCCG TCAGCGACCT CCTGAGTGAC 
ACGGAAGAAG ACGACAAATG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330733.1
CDS	808..7731
Translation	MARFGEAVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECRDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNAGTSALEVLRRATIKRSRTEAMARDSSDEHCVDISSVGTPLARASIKSAKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSLVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLSAHPSLYRRPRAIEGLALGLGLEKCEEERVSRGGSLKGDGGDLASALDSQRSPLALGKREPPWLARPCHGNCDPTQPEAGSGETVVTFGDRARHRQSQRRSRHRRVRTEGREPSSTSRSRSASQERSLDEAVPSEGERDREPRAGHGGKEPMIQEEERERCQDLRRTNSLMVPRGSGLAGALDEADTPLVPPQPELEVGKDAALTEQEPEGSSEQALLGDVKLDIGRVSQSEPDLSCVTANTDKATTESTSVTVTIPDVGPLVDSTVVHISNKTDGEASPLKEAEIREDEEEVEKKQQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALATNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKIIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQSESERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVPGLSGRSAYPSMSPLSPQEIFQLACMDPADDGQFQERQSLEPEVSELKSVQPSNHGIYLPSDTQEHAGSGKASSMPRLTVDPQVVTDPSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERHQSRSPSEGRSQTPNRQGTGSLSESSIPSVSDSSTPRRSRRQLPPVPPKPRPLLSYSSLIRHAGSISPPADGSEGGSPLTSQALESNDAGLPESSNPRQGHPSPQRYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGTTCGAVSDLLSDTEEDDKC

Target ORF information:

RefSeq Version	XM_015475247.2
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015475247.2

ORF Insert Sequence:

ATGGCTCGCT TCGGGGAGGC GGTGGTCGGC AGGCCGGGGT CGGGCGATGG AGACTCGGAC 
CAGAGCAGGA ACCGGCAAGG AACCCCCGTG CCGGCCTCCG GGCCGGCGGC CGCCTACAAG 
CAGTCGAAAG CGCAGAGGGC GCGGACTATG GCTTTGTACA ACCCTATTCC CGTCCGGCAG 
AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA 
TACGCCAAGA AGCTCATCGA TTGGCCGCCG TTTGAGTACA TGATCTTGGC CACCATCATT 
GCCAATTGCA TCGTCTTGGC CCTGGAGCAA CATCTTCCTG AGGATGACAA GACCCCAATG 
TCCCGCAGAC TGGAGAAGAC AGAACCCTAT TTCATTGGGA TCTTTTGCTT TGAAGCTGGG 
ATCAAAATCG TGGCCCTGGG GTTCATCTTC CATAAGGGAT CATACCTCCG CAATGGTTGG 
AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CTACTGCGGG AACCCACTTC 
AACACCCATG TGGACCTGAG GACCCTCCGG GCCGTGCGTG TCCTGAGGCC CCTGAAGCTC 
GTGTCGGGGA TACCTAGCCT ACAGATTGTG TTGAAATCCA TCATGAAGGC CATGGTGCCT 
CTCCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATCCTGA TGTTTGCTAT CATCGGCTTG 
GAGTTCTACA GTGGAAAGTT GCACCGAGCG TGCTTCATGA ACAATTCAGG TATTCTAGAA 
GGATTTGACC CCCCTCACCC ATGTGGCGTG CAGGGCTGCC CAGCTGGTTA TGAATGCCGG 
GACTGGATCG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCCGTGCTG 
ACCGTCTTCC AGTGTATCAC CATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT 
GCCTTAGGAG CCACCTGGAA TTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC 
TTTGTTCTCA ACCTTGTCCT GGGAGTGCTT TCCGGGGAAT TTGCCAAAGA GAGAGAGAGA 
GTGGAGAACC GGAGAGCCTT CATGAAACTG CGGCGCCAGC AGCAGATCGA GCGTGAGCTT 
AACGGGTACC GCGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTTGCTGA AGAAAACAAA 
AATGCTGGAA CGTCAGCCTT AGAAGTGCTT CGGAGGGCAA CCATCAAAAG GAGCCGGACC 
GAGGCCATGG CACGGGACTC CAGCGATGAG CACTGCGTGG ACATCTCCTC TGTGGGCACA 
CCTCTCGCCC GAGCCAGCAT CAAGAGCGCC AAGGTGGACG GGGCCTCCTA TTTCCGGCAC 
AAGGAGCGAC TTTTGCGGAT CTCTATCCGC CACATGGTTA AGTCCCAGGT GTTCTACTGG 
ATCGTGCTGA GCCTGGTGGC TCTCAACACC GCCTGTGTGG CCATCGTCCA CCACAACCAG 
CCCCAGTGGC TCACCCACCT CCTCTACTAT GCAGAGTTTC TGTTTCTGGG GCTCTTCCTT 
CTAGAGATGT CCCTGAAGAT GTACGGCATG GGGCCTCGCC TTTATTTTCA CTCTTCCTTC 
AACTGCTTTG ACTTTGGGGT CACAGTGGGC AGCATCTTTG AAGTAGTCTG GGCCATCTTC 
AGACCTGGTA CATCTTTCGG AATCAGTGTC CTGCGAGCTC TCCGGCTTCT AAGAATATTT 
AAAATAACCA AGTATTGGGC ATCCCTCCGG AATTTGGTGG TCTCCTTGAT GAGTTCCATG 
AAGTCTATCA TCAGTTTACT CTTCCTCCTT TTCCTCTTCA TTGTTGTCTT TGCTCTGCTA 
GGAATGCAGC TGTTTGGGGG CAGGTTCAAC TTTAATGATG GGACTCCTTC CGCAAACTTT 
GACACCTTCC CTGCAGCCAT CATGACTGTA TTCCAGATCC TGACGGGTGA GGACTGGAAT 
GAGGTGATGT ACAACGGGAT CCGCTCCCAG GGTGGGGTCA GCTCGGGCAT GTGGTCTGCG 
ATCTATTTCA TTGTGCTTAC CTTGTTTGGA AACTACACAC TGCTGAATGT GTTCTTGGCC 
ATCGCTGTGG ACAATCTGGC CAACGCCCAG GAGCTGACCA AGGATGAACA AGAAGAAGAG 
GAGGCCTTCA ACCAGAAGCA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA 
CCCAACATGC CTTCCATCGA AAGAGACAGA AGGAGAAGAC ACCACATGTC GATGTGGGAG 
CCACGCAGCA GCCACCTGAG GGAGCGGAGG CGCCGGCATC ACATGTCCGT GTGGGAGCAG 
CGAACCAGCC AGTTGAGGAG GCACATGCAG ATGTCCAGCC AGGAGGCCCT CAACAAGGAG 
GAGGCCCCGC CCATGAACCC CCTCAACCCC CTCAACCCGC TGAGCCCCCT CAACCCGCTC 
AGCGCGCACC CCAGCCTCTA CCGGAGACCC CGGGCCATCG AGGGGCTAGC CCTGGGCCTG 
GGGCTGGAGA AGTGTGAGGA AGAGCGCGTC AGCCGTGGGG GGTCCCTCAA AGGGGATGGA 
GGGGACTTGG CGAGCGCCCT GGACAGCCAG AGGAGCCCCC TGGCCCTGGG CAAACGGGAG 
CCACCCTGGC TGGCCCGGCC CTGTCACGGG AACTGTGACC CGACCCAGCC GGAGGCGGGC 
AGTGGGGAGA CGGTGGTGAC CTTCGGGGAC CGGGCCCGGC ACAGGCAGAG CCAGCGGCGC 
AGCCGGCACC GCCGCGTCAG GACCGAGGGC AGGGAGCCCT CCTCCACCTC CAGAAGCAGG 
TCTGCCAGCC AGGAGCGGAG TCTGGACGAA GCTGTGCCCA GCGAGGGGGA GAGGGACCGT 
GAGCCCAGGG CTGGCCATGG GGGCAAAGAA CCCATGATCC AGGAGGAAGA GCGAGAGCGA 
TGCCAGGACT TACGGAGGAC CAACAGTCTG ATGGTGCCCA GAGGCTCCGG GCTGGCAGGA 
GCCCTGGATG AGGCCGACAC ACCCCTTGTC CCACCCCAGC CGGAGCTGGA AGTGGGGAAG 
GACGCAGCCC TGACGGAGCA GGAGCCGGAG GGCAGCAGTG AGCAGGCTCT GCTGGGGGAC 
GTGAAGCTGG ACATAGGCCG GGTCAGCCAG AGCGAGCCCG ACCTCTCCTG CGTCACGGCC 
AACACGGACA AGGCCACCAC CGAGAGCACC AGCGTCACTG TCACCATCCC CGACGTGGGC 
CCCTTGGTGG ACTCAACCGT GGTGCACATC AGCAACAAGA CTGATGGAGA AGCCAGTCCC 
TTGAAAGAGG CAGAGATCAG AGAGGATGAG GAGGAGGTGG AGAAGAAGCA GCAGAAGAAG 
GAGAAGCGTG AGACGGGCAA GGCCATGGTG CCCCACAGCT CGATGTTCAT CTTCAGCACC 
ACCAACCCGA TCCGCAGGGC CTGCCACTAC ATCGTGAACC TGCGCTACTT CGAGATGTGC 
ATCCTCCTGG TGATCGCAGC CAGCAGCATC GCCCTGGCGG CCGAGGACCC CGTCCTGACC 
AACTCAGAGC GCAACAAAGT GCTGAGGTAT TTTGACTATG TGTTCACTGG CGTGTTCACC 
TTTGAGATGG TTATAAAGAT GATAGACCAG GGCTTGATTC TGCAGGACGG CTCCTACTTC 
CGAGACCTGT GGAACATCCT GGACTTCGTG GTGGTGGTTG GCGCATTGGT GGCCTTTGCT 
CTGGCAACTA ACAAGGGCCG GGATATTAAG ACCATCAAGT CCCTGAGGGT TCTCCGTGTC 
CTGAGGCCGC TGAAGACCAT CAAGCGCCTG CCCAAGCTCA AGGCTGTCTT TGACTGTGTG 
GTCACCTCCT TGAAAAACGT CTTCAACATA CTCATTGTCT ACAAGCTCTT CATGTTCATC 
TTTGCTGTCA TCGCAGTTCA GCTTTTCAAG GGCAAGTTCT TCTACTGCAC GGACAGTTCC 
AAGGACACAG AGAAGGAGTG CATAGGCAAC TATGTAGATC ACGAGAAAAA CAAGATGGAG 
GTGAAAGGCC GAGAGTGGAA GCGCCATGAA TTCCATTATG ACAACATCAT CTGGGCTCTG 
CTGACCCTCT TCACTGTCTC CACAGGGGAA GGGTGGCCTC AGGTCTTGCA GCACTCGGTC 
GATGTGACAG AGGAAGACCG AGGCCCAAGC CGCAGCAACC GGATGGAGAT GTCCATCTTT 
TATGTGGTCT ACTTTGTGGT CTTCCCCTTC TTCTTTGTCA ACATCTTTGT GGCTCTCATC 
ATCATCACCT TCCAGGAGCA AGGGGACAAG ATGATGGAAG AGTGCAGCCT GGAGAAGAAT 
GAGAGGGCAT GCATCGACTT CGCCATCAGC GCCAAACCGC TCACCCGCTA CATGCCCCAG 
AACAGGCACA CCTTCCAGTA CCGCGTCTGG CACTTCGTGG TGTCTCCATC CTTTGAGTAC 
ACCATCATGG CCATGATCGC CCTGAACACC GTCGTGCTGA TGATGAAGTA CTACTCTGCT 
CCCTGTACCT ATGAGCTGGC CCTGAAGTAC CTAAATATCG CCTTCACCAT GGTGTTTTCC 
CTGGAATGTG TCCTGAAGAT CATTGCATTT GGCTTCTTGA ACTATTTCCG AGACACCTGG 
AATATCTTTG ACTTCATCAC TGTGATTGGC AGTATCACAG AAATTATTCT GACGGACAGC 
AAGCTGGTGA ACACTAGTGG CTTCAACATG AGCTTTCTGA AGCTCTTCCG AGCGGCCCGC 
CTCATCAAGC TGCTGCGTCA GGGCTACACC ATCCGCATTT TACTGTGGAC CTTCGTGCAG 
TCCTTTAAGG CCCTTCCCTA TGTCTGCCTT CTGATCGCCA TGCTTTTCTT CATCTATGCC 
ATCATTGGGA TGCAGGTCTT TGGAAACATA AAGTTAGATG AGGAGAGTCA CATCAACCGG 
CACAACAACT TCCGGAGTTT CTTTGGGTCC CTCATGCTGC TCTTCAGGAG TGCCACGGGT 
GAGGCCTGGC AGGAGATAAT GCTGTCGTGT CTCGGGGAGA AGGGCTGTGA GCCCGACACC 
ACTGCGCCGT CGGGGCAGAG CGAGAGCGAG CGCTGTGGCA CCGACCTGGC CTACGTGTAC 
TTCGTCTCCT TCATCTTCTT CTGCTCCTTC CTGATGCTCA ACCTGTTCGT GGCTGTCATC 
ATGGACAACT TTGAGTACCT GACTCGGGAC TCGTCCATCC TGGGGCCTCA CCATCTGGAC 
GAGTTTGTCC GAGTCTGGGC AGAGTATGAC AGAGCAGCAT GTGGCCGCAT CCATTACACT 
GAGATGTATG AAATGCTGAC TCTCATGTCA CCGCCGCTAG GCCTCGGCAA GAGATGTCCC 
TCCAAGGTGG CATATAAGAG GTTGGTCTTG ATGAACATGC CAGTGGCCGA GGACATGACC 
GTCCACTTCA CTTCCACGCT GATGGCTCTA ATCCGGACAG CTCTGGACAT TAAAATCGCC 
AAAGGCGGTG CAGACAGGCA GCAGCTAGAC TCAGAGCTCC AGAAGGAGAC CCTGGCCATC 
TGGCCTCACC TGTCCCAGAA GATGCTGGAT CTGCTCGTGC CCATGCCCAA AGCCTCTGAC 
CTGACTGTGG GCAAAATCTA TGCAGCAATG ATGATCATGG ACTACTACAA ACAGAGCAAG 
GTGAAGAAGC AGAGGCAGCA GCTGGAGGAA CAGAAAAACG CACCCATGTT CCAGCGCATG 
GAGCCCTCGT CTCTGCCTCA GGAGATCATC GCTAATGCCA AAGCCCTGCC TTATCTCCAG 
CAGGACCCCG TTCCAGGCCT GAGTGGCCGG AGTGCATACC CTTCGATGAG TCCACTCTCC 
CCGCAGGAAA TATTCCAGTT GGCCTGTATG GACCCAGCTG ATGATGGACA GTTCCAGGAA 
CGACAATCTC TGGAGCCAGA GGTTAGTGAA TTAAAAAGCG TGCAGCCCTC TAACCATGGC 
ATCTACCTTC CTTCGGACAC CCAGGAGCAT GCGGGCTCTG GGAAGGCATC CTCTATGCCA 
CGCCTGACTG TGGATCCCCA GGTGGTGACA GACCCCAGCT CCATGAGACG TTCATTTTCC 
ACCATTCGCG ATAAGCGTTC AAATTCCTCG TGGCTGGAGG AATTCTCCAT GGAGCGAAGC 
AGCGAAAACA CCTACAAGTC CCGGCGCCGG AGTTACCACT CCTCCCTGAG GCTGTCAGCC 
CACCGTCTGA ACTCGGACTC AGGCCACAAG TCCGACACCC ACCGCTCAGG GGGCAGAGAG 
CGGGGACGAT CGAAAGAGCG AAAGCATCTT CTCTCTCCTG ACGTCTCCCG CTGCAACTCC 
GAGGAGCGAG GGACCCAGGC CGACTGGGAG TCCCCAGAGC GCCATCAGTC CCGGTCTCCC 
AGTGAAGGCA GGTCACAGAC CCCCAACAGA CAGGGCACGG GGTCCCTGAG TGAGAGCTCC 
ATCCCCTCTG TCTCCGACTC CAGCACCCCA CGACGAAGTC GGCGGCAGCT CCCCCCAGTC 
CCTCCCAAGC CTCGGCCCCT CCTTTCCTAC AGCTCTCTGA TCAGACACGC TGGGAGCATC 
TCCCCACCAG CTGATGGGAG CGAGGGTGGC TCCCCACTGA CCTCCCAAGC TCTGGAGAGC 
AACGACGCCG GCCTGCCCGA GTCTTCCAAC CCCCGGCAGG GCCACCCCTC CCCACAGCGC 
TACATCTCCG AGCCCTACCT GGCCCTGCAC GAAGACTCCC ACGCCTCGGA CTGCGGCGAG 
GAGGAGACGC TCACCTTTGA AGCAGCCGTG GCCACCAGCC TGGGCCGGTC CAACACCATC 
GGCTCGGCCC CGCCCCTGCG GCACAGCTGG CAGATGCCCA ATGGGCACTA TCGGCGGCGG 
AGGCGCGGGG GGCCTGGACC GGGCACGACG TGCGGAGCCG TCAGCGACCT CCTGAGTGAC 
ACGGAAGAAG ACGACAAATG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1E ( XM_015475247.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330733.1 Bos taurus calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X2, mRNA.

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CloneID	OBa93037
Clone ID Related Accession (Same CDS sequence)	XM_015475247.1
Accession Version	XM_015475247.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6924bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104440.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGCT TCGGGGAGGC GGTGGTCGGC AGGCCGGGGT CGGGCGATGG AGACTCGGAC 
CAGAGCAGGA ACCGGCAAGG AACCCCCGTG CCGGCCTCCG GGCCGGCGGC CGCCTACAAG 
CAGTCGAAAG CGCAGAGGGC GCGGACTATG GCTTTGTACA ACCCTATTCC CGTCCGGCAG 
AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA 
TACGCCAAGA AGCTCATCGA TTGGCCGCCG TTTGAGTACA TGATCTTGGC CACCATCATT 
GCCAATTGCA TCGTCTTGGC CCTGGAGCAA CATCTTCCTG AGGATGACAA GACCCCAATG 
TCCCGCAGAC TGGAGAAGAC AGAACCCTAT TTCATTGGGA TCTTTTGCTT TGAAGCTGGG 
ATCAAAATCG TGGCCCTGGG GTTCATCTTC CATAAGGGAT CATACCTCCG CAATGGTTGG 
AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CTACTGCGGG AACCCACTTC 
AACACCCATG TGGACCTGAG GACCCTCCGG GCCGTGCGTG TCCTGAGGCC CCTGAAGCTC 
GTGTCGGGGA TACCTAGCCT ACAGATTGTG TTGAAATCCA TCATGAAGGC CATGGTGCCT 
CTCCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATCCTGA TGTTTGCTAT CATCGGCTTG 
GAGTTCTACA GTGGAAAGTT GCACCGAGCG TGCTTCATGA ACAATTCAGG TATTCTAGAA 
GGATTTGACC CCCCTCACCC ATGTGGCGTG CAGGGCTGCC CAGCTGGTTA TGAATGCCGG 
GACTGGATCG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCCGTGCTG 
ACCGTCTTCC AGTGTATCAC CATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT 
GCCTTAGGAG CCACCTGGAA TTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC 
TTTGTTCTCA ACCTTGTCCT GGGAGTGCTT TCTGGGGAAT TTGCCAAAGA GAGAGAGAGA 
GTGGAGAACC GGAGAGCCTT CATGAAACTG CGGCGCCAGC AGCAGATCGA GCGTGAGCTT 
AACGGGTACC GCGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTTGCTGA AGAAAACAAA 
AATGCTGGAA CGTCAGCCTT AGAAGTGCTT CGGAGGGCAA CCATCAAAAG GAGCCGGACC 
GAGGCCATGG CACGGGACTC CAGCGATGAG CACTGCGTGG ACATCTCCTC TGTGGGCACA 
CCTCTCGCCC GAGCCAGCAT CAAGAGCGCC AAGGTGGACG GGGCCTCCTA TTTCCGGCAC 
AAGGAGCGAC TTTTGCGGAT CTCTATCCGC CACATGGTTA AGTCCCAGGT GTTCTACTGG 
ATCGTGCTGA GCCTGGTGGC TCTCAACACC GCCTGTGTGG CCATCGTCCA CCACAACCAG 
CCCCAGTGGC TCACCCACCT CCTCTACTAT GCAGAGTTTC TGTTTCTGGG GCTCTTCCTT 
CTAGAGATGT CCCTGAAGAT GTACGGCATG GGGCCTCGCC TTTATTTTCA CTCTTCCTTC 
AACTGCTTTG ACTTTGGGGT CACAGTGGGC AGCATCTTTG AAGTAGTCTG GGCCATCTTC 
AGACCTGGTA CATCTTTCGG AATCAGTGTC CTGCGAGCTC TCCGGCTTCT AAGAATATTT 
AAAATAACCA AGTATTGGGC ATCCCTCCGG AATTTGGTGG TCTCCTTGAT GAGTTCCATG 
AAGTCTATCA TCAGTTTACT CTTCCTCCTT TTCCTCTTCA TTGTTGTCTT TGCTCTGCTA 
GGAATGCAGC TGTTTGGGGG CAGGTTCAAC TTTAATGATG GGACTCCTTC CGCAAACTTT 
GACACCTTCC CTGCAGCCAT CATGACTGTA TTCCAGATCC TGACGGGTGA GGACTGGAAT 
GAGGTGATGT ACAACGGGAT CCGCTCCCAG GGTGGGGTCA GCTCGGGCAT GTGGTCTGCG 
ATCTATTTCA TTGTGCTTAC CTTGTTTGGA AACTACACAC TGCTGAATGT GTTCTTGGCC 
ATCGCTGTGG ACAATCTGGC CAACGCCCAG GAGCTGACCA AGGATGAACA AGAAGAAGAG 
GAGGCCTTCA ACCAGAAGCA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA 
CCCAACATGC CTTCCATCGA AAGAGACAGA AGGAGAAGAC ACCACATGTC GATGTGGGAG 
CCACGCAGCA GCCACCTGAG GGAGCGGAGG CGCCGGCATC ACATGTCCGT GTGGGAGCAG 
CGAACCAGCC AGTTGAGGAG GCACATGCAG ATGTCCAGCC AGGAGGCCCT CAACAAGGAG 
GAGGCCCCGC CCATGAACCC CCTCAACCCC CTCAACCCGC TGAGCCCCCT CAACCCGCTC 
AGCGCGCACC CCAGCCTCTA CCGGAGACCC CGGGCCATCG AGGGGCTAGC CCTGGGCCTG 
GGGCTGGAGA AGTGTGAGGA AGAGCGCGTC AGCCGTGGGG GGTCCCTCAA AGGGGATGGA 
GGGGACTTGG CGAGCGCCCT GGACAGCCAG AGGAGCCCCC TGGCCCTGGG CAAACGGGAG 
CCACCCTGGC TGGCCCGGCC CTGTCACGGG AACTGTGACC CGACCCAGCC GGAGGCGGGC 
AGTGGGGAGA CGGTGGTGAC CTTCGGGGAC CGGGCCCGGC ACAGGCAGAG CCAGCGGCGC 
AGCCGGCACC GCCGCGTCAG GACCGAGGGC AGGGAGCCCT CCTCCACCTC CAGAAGCAGG 
TCTGCCAGCC AGGAGCGGAG TCTGGACGAA GCTGTGCCCA GCGAGGGGGA GAGGGACCGT 
GAGCCCAGGG CTGGCCATGG GGGCAAAGAA CCCATGATCC AGGAGGAAGA GCGAGAGCGA 
TGCCAGGACT TACGGAGGAC CAACAGTCTG ATGGTGCCCA GAGGCTCCGG GCTGGCAGGA 
GCCCTGGATG AGGCCGACAC ACCCCTTGTC CCACCCCAGC CGGAGCTGGA AGTGGGGAAG 
GACGCAGCCC TGACGGAGCA GGAGCCGGAG GGCAGCAGTG AGCAGGCTCT GCTGGGGGAC 
GTGAAGCTGG ACATAGGCCG GGTCAGCCAG AGCGAGCCCG ACCTCTCCTG CGTCACGGCC 
AACACGGACA AGGCCACCAC CGAGAGCACC AGCGTCACTG TCACCATCCC CGACGTGGGC 
CCCTTGGTGG ACTCAACCGT GGTGCACATC AGCAACAAGA CTGATGGAGA AGCCAGTCCC 
TTGAAAGAGG CAGAGATCAG AGAGGATGAG GAGGAGGTGG AGAAGAAGCA GCAGAAGAAG 
GAGAAGCGTG AGACGGGCAA GGCCATGGTG CCCCACAGCT CGATGTTCAT CTTCAGCACC 
ACCAACCCGA TCCGCAGGGC CTGCCACTAC ATCGTGAACC TGCGCTACTT CGAGATGTGC 
ATCCTCCTGG TGATCGCAGC CAGCAGCATC GCCCTGGCGG CCGAGGACCC CGTCCTGACC 
AACTCAGAGC GCAACAAAGT GCTGAGGTAT TTTGACTATG TGTTCACTGG CGTGTTCACC 
TTTGAGATGG TTATAAAGAT GATAGACCAG GGCTTGATTC TGCAGGACGG CTCCTACTTC 
CGAGACCTGT GGAACATCCT GGACTTCGTG GTGGTGGTTG GCGCATTGGT GGCCTTTGCT 
CTGGCAACTA ACAAGGGCCG GGATATTAAG ACCATCAAGT CCCTGAGGGT TCTCCGTGTC 
CTGAGGCCGC TGAAGACCAT CAAGCGCCTG CCCAAGCTCA AGGCTGTCTT TGACTGTGTG 
GTCACCTCCT TGAAAAACGT CTTCAACATA CTCATTGTCT ACAAGCTCTT CATGTTCATC 
TTTGCTGTCA TCGCAGTTCA GCTTTTCAAG GGCAAGTTCT TCTACTGCAC GGACAGTTCC 
AAGGACACAG AGAAGGAGTG CATAGGCAAC TATGTAGATC ACGAGAAAAA CAAGATGGAG 
GTGAAAGGCC GAGAGTGGAA GCGCCATGAA TTCCATTATG ACAACATCAT CTGGGCTCTG 
CTGACCCTCT TCACTGTCTC CACAGGGGAA GGGTGGCCTC AGGTCTTGCA GCACTCGGTC 
GATGTGACAG AGGAAGACCG AGGCCCAAGC CGCAGCAACC GGATGGAGAT GTCCATCTTT 
TATGTGGTCT ACTTTGTGGT CTTCCCCTTC TTCTTTGTCA ACATCTTTGT GGCTCTCATC 
ATCATCACCT TCCAGGAGCA AGGGGACAAG ATGATGGAAG AGTGCAGCCT GGAGAAGAAT 
GAGAGGGCAT GCATCGACTT CGCCATCAGC GCCAAACCGC TCACCCGCTA CATGCCCCAG 
AACAGGCACA CCTTCCAGTA CCGCGTCTGG CACTTCGTGG TGTCTCCATC CTTTGAGTAC 
ACCATCATGG CCATGATCGC CCTGAACACC GTCGTGCTGA TGATGAAGTA CTACTCTGCT 
CCCTGTACCT ATGAGCTGGC CCTGAAGTAC CTAAATATCG CCTTCACCAT GGTGTTTTCC 
CTGGAATGTG TCCTGAAGAT CATTGCATTT GGCTTCTTGA ACTATTTCCG AGACACCTGG 
AATATCTTTG ACTTCATCAC TGTGATTGGC AGTATCACAG AAATTATTCT GACGGACAGC 
AAGCTGGTGA ACACTAGTGG CTTCAACATG AGCTTTCTGA AGCTCTTCCG AGCGGCCCGC 
CTCATCAAGC TGCTGCGTCA GGGCTACACC ATCCGCATTT TACTGTGGAC CTTCGTGCAG 
TCCTTTAAGG CCCTTCCCTA TGTCTGCCTT CTGATCGCCA TGCTTTTCTT CATCTATGCC 
ATCATTGGGA TGCAGGTCTT TGGAAACATA AAGTTAGATG AGGAGAGTCA CATCAACCGG 
CACAACAACT TCCGGAGTTT CTTTGGGTCC CTCATGCTGC TCTTCAGGAG TGCCACGGGT 
GAGGCCTGGC AGGAGATAAT GCTGTCGTGT CTCGGGGAGA AGGGCTGTGA GCCCGACACC 
ACTGCGCCGT CGGGGCAGAG CGAGAGCGAG CGCTGTGGCA CCGACCTGGC CTACGTGTAC 
TTCGTCTCCT TCATCTTCTT CTGCTCCTTC CTGATGCTCA ACCTGTTCGT GGCTGTCATC 
ATGGACAACT TTGAGTACCT GACTCGGGAC TCGTCCATCC TGGGGCCTCA CCATCTGGAC 
GAGTTTGTCC GAGTCTGGGC AGAGTATGAC AGAGCAGCAT GTGGCCGCAT CCATTACACT 
GAGATGTATG AAATGCTGAC TCTCATGTCA CCGCCGCTAG GCCTCGGCAA GAGATGTCCC 
TCCAAGGTGG CATATAAGAG GTTGGTCTTG ATGAACATGC CAGTGGCCGA GGACATGACC 
GTCCACTTCA CTTCCACGCT GATGGCTCTA ATCCGGACAG CTCTGGACAT TAAAATCGCC 
AAAGGCGGTG CAGACAGGCA GCAGCTAGAC TCAGAGCTCC AGAAGGAGAC CCTGGCCATC 
TGGCCTCACC TGTCCCAGAA GATGCTGGAT CTGCTCGTGC CCATGCCCAA AGCCTCTGAC 
CTGACTGTGG GCAAAATCTA TGCAGCAATG ATGATCATGG ACTACTACAA ACAGAGCAAG 
GTGAAGAAGC AGAGGCAGCA GCTGGAGGAA CAGAAAAACG CACCCATGTT CCAGCGCATG 
GAGCCCTCGT CTCTGCCTCA GGAGATCATC GCTAATGCCA AAGCCCTGCC TTATCTCCAG 
CAGGACCCCG TTCCAGGCCT GAGTGGCCGG AGTGCATACC CTTCGATGAG TCCACTCTCC 
CCGCAGGAAA TATTCCAGTT GGCCTGTATG GACCCAGCTG ATGATGGACA GTTCCAGGAA 
CGACAATCTC TGGAGCCAGA GGTTAGTGAA TTAAAAAGCG TGCAGCCCTC TAACCATGGC 
ATCTACCTTC CTTCGGACAC CCAGGAGCAT GCGGGCTCTG GGAAGGCATC CTCTATGCCA 
CGCCTGACTG TGGATCCCCA GGTGGTGACA GACCCCAGCT CCATGAGACG TTCATTTTCC 
ACCATTCGCG ATAAGCGTTC AAATTCCTCG TGGCTGGAGG AATTCTCCAT GGAGCGAAGC 
AGCGAAAACA CCTACAAGTC CCGGCGCCGG AGTTACCACT CCTCCCTGAG GCTGTCAGCC 
CACCGTCTGA ACTCGGACTC AGGCCACAAG TCCGACACCC ACCGCTCAGG GGGCAGAGAG 
CGGGGACGAT CGAAAGAGCG AAAGCATCTT CTCTCTCCTG ACGTCTCCCG CTGCAACTCC 
GAGGAGCGAG GGACCCAGGC CGACTGGGAG TCCCCAGAGC GCCATCAGTC CCGGTCTCCC 
AGTGAAGGCA GGTCACAGAC CCCCAACAGA CAGGGCACGG GGTCCCTGAG TGAGAGCTCC 
ATCCCCTCTG TCTCCGACTC CAGCACCCCA CGACGAAGTC GGCGGCAGCT CCCCCCAGTC 
CCTCCCAAGC CTCGGCCCCT CCTTTCCTAC AGCTCTCTGA TCAGACACGC TGGGAGCATC 
TCCCCACCAG CTGATGGGAG CGAGGGTGGC TCCCCACTGA CCTCCCAAGC TCTGGAGAGC 
AACGACGCCG GCCTGCCCGA GTCTTCCAAC CCCCGGCAGG GCCACCCCTC CCCACAGCGC 
TACATCTCCG AGCCCTACCT GGCCCTGCAC GAAGACTCCC ACGCCTCGGA CTGCGGCGAG 
GAGGAGACGC TCACCTTTGA AGCAGCCGTG GCCACCAGCC TGGGCCGGTC CAACACCATC 
GGCTCGGCCC CGCCCCTGCG GCACAGCTGG CAGATGCCCA ATGGGCACTA TCGGCGGCGG 
AGGCGCGGGG GGCCTGGACC GGGCACGACG TGCGGAGCCG TCAGCGACCT CCTGAGTGAC 
ACGGAAGAAG ACGACAAATG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330733.1
CDS	165..7088
Translation	MARFGEAVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECRDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNAGTSALEVLRRATIKRSRTEAMARDSSDEHCVDISSVGTPLARASIKSAKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSLVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLSAHPSLYRRPRAIEGLALGLGLEKCEEERVSRGGSLKGDGGDLASALDSQRSPLALGKREPPWLARPCHGNCDPTQPEAGSGETVVTFGDRARHRQSQRRSRHRRVRTEGREPSSTSRSRSASQERSLDEAVPSEGERDREPRAGHGGKEPMIQEEERERCQDLRRTNSLMVPRGSGLAGALDEADTPLVPPQPELEVGKDAALTEQEPEGSSEQALLGDVKLDIGRVSQSEPDLSCVTANTDKATTESTSVTVTIPDVGPLVDSTVVHISNKTDGEASPLKEAEIREDEEEVEKKQQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALATNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKIIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQSESERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVPGLSGRSAYPSMSPLSPQEIFQLACMDPADDGQFQERQSLEPEVSELKSVQPSNHGIYLPSDTQEHAGSGKASSMPRLTVDPQVVTDPSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERHQSRSPSEGRSQTPNRQGTGSLSESSIPSVSDSSTPRRSRRQLPPVPPKPRPLLSYSSLIRHAGSISPPADGSEGGSPLTSQALESNDAGLPESSNPRQGHPSPQRYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGTTCGAVSDLLSDTEEDDKC

Target ORF information:

RefSeq Version	XM_015475247.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015475247.1

ORF Insert Sequence:

ATGGCTCGCT TCGGGGAGGC GGTGGTCGGC AGGCCGGGGT CGGGCGATGG AGACTCGGAC 
CAGAGCAGGA ACCGGCAAGG AACCCCCGTG CCGGCCTCCG GGCCGGCGGC CGCCTACAAG 
CAGTCGAAAG CGCAGAGGGC GCGGACTATG GCTTTGTACA ACCCTATTCC CGTCCGGCAG 
AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA 
TACGCCAAGA AGCTCATCGA TTGGCCGCCG TTTGAGTACA TGATCTTGGC CACCATCATT 
GCCAATTGCA TCGTCTTGGC CCTGGAGCAA CATCTTCCTG AGGATGACAA GACCCCAATG 
TCCCGCAGAC TGGAGAAGAC AGAACCCTAT TTCATTGGGA TCTTTTGCTT TGAAGCTGGG 
ATCAAAATCG TGGCCCTGGG GTTCATCTTC CATAAGGGAT CATACCTCCG CAATGGTTGG 
AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CTACTGCGGG AACCCACTTC 
AACACCCATG TGGACCTGAG GACCCTCCGG GCCGTGCGTG TCCTGAGGCC CCTGAAGCTC 
GTGTCGGGGA TACCTAGCCT ACAGATTGTG TTGAAATCCA TCATGAAGGC CATGGTGCCT 
CTCCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATCCTGA TGTTTGCTAT CATCGGCTTG 
GAGTTCTACA GTGGAAAGTT GCACCGAGCG TGCTTCATGA ACAATTCAGG TATTCTAGAA 
GGATTTGACC CCCCTCACCC ATGTGGCGTG CAGGGCTGCC CAGCTGGTTA TGAATGCCGG 
GACTGGATCG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCCGTGCTG 
ACCGTCTTCC AGTGTATCAC CATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT 
GCCTTAGGAG CCACCTGGAA TTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC 
TTTGTTCTCA ACCTTGTCCT GGGAGTGCTT TCTGGGGAAT TTGCCAAAGA GAGAGAGAGA 
GTGGAGAACC GGAGAGCCTT CATGAAACTG CGGCGCCAGC AGCAGATCGA GCGTGAGCTT 
AACGGGTACC GCGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTTGCTGA AGAAAACAAA 
AATGCTGGAA CGTCAGCCTT AGAAGTGCTT CGGAGGGCAA CCATCAAAAG GAGCCGGACC 
GAGGCCATGG CACGGGACTC CAGCGATGAG CACTGCGTGG ACATCTCCTC TGTGGGCACA 
CCTCTCGCCC GAGCCAGCAT CAAGAGCGCC AAGGTGGACG GGGCCTCCTA TTTCCGGCAC 
AAGGAGCGAC TTTTGCGGAT CTCTATCCGC CACATGGTTA AGTCCCAGGT GTTCTACTGG 
ATCGTGCTGA GCCTGGTGGC TCTCAACACC GCCTGTGTGG CCATCGTCCA CCACAACCAG 
CCCCAGTGGC TCACCCACCT CCTCTACTAT GCAGAGTTTC TGTTTCTGGG GCTCTTCCTT 
CTAGAGATGT CCCTGAAGAT GTACGGCATG GGGCCTCGCC TTTATTTTCA CTCTTCCTTC 
AACTGCTTTG ACTTTGGGGT CACAGTGGGC AGCATCTTTG AAGTAGTCTG GGCCATCTTC 
AGACCTGGTA CATCTTTCGG AATCAGTGTC CTGCGAGCTC TCCGGCTTCT AAGAATATTT 
AAAATAACCA AGTATTGGGC ATCCCTCCGG AATTTGGTGG TCTCCTTGAT GAGTTCCATG 
AAGTCTATCA TCAGTTTACT CTTCCTCCTT TTCCTCTTCA TTGTTGTCTT TGCTCTGCTA 
GGAATGCAGC TGTTTGGGGG CAGGTTCAAC TTTAATGATG GGACTCCTTC CGCAAACTTT 
GACACCTTCC CTGCAGCCAT CATGACTGTA TTCCAGATCC TGACGGGTGA GGACTGGAAT 
GAGGTGATGT ACAACGGGAT CCGCTCCCAG GGTGGGGTCA GCTCGGGCAT GTGGTCTGCG 
ATCTATTTCA TTGTGCTTAC CTTGTTTGGA AACTACACAC TGCTGAATGT GTTCTTGGCC 
ATCGCTGTGG ACAATCTGGC CAACGCCCAG GAGCTGACCA AGGATGAACA AGAAGAAGAG 
GAGGCCTTCA ACCAGAAGCA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA 
CCCAACATGC CTTCCATCGA AAGAGACAGA AGGAGAAGAC ACCACATGTC GATGTGGGAG 
CCACGCAGCA GCCACCTGAG GGAGCGGAGG CGCCGGCATC ACATGTCCGT GTGGGAGCAG 
CGAACCAGCC AGTTGAGGAG GCACATGCAG ATGTCCAGCC AGGAGGCCCT CAACAAGGAG 
GAGGCCCCGC CCATGAACCC CCTCAACCCC CTCAACCCGC TGAGCCCCCT CAACCCGCTC 
AGCGCGCACC CCAGCCTCTA CCGGAGACCC CGGGCCATCG AGGGGCTAGC CCTGGGCCTG 
GGGCTGGAGA AGTGTGAGGA AGAGCGCGTC AGCCGTGGGG GGTCCCTCAA AGGGGATGGA 
GGGGACTTGG CGAGCGCCCT GGACAGCCAG AGGAGCCCCC TGGCCCTGGG CAAACGGGAG 
CCACCCTGGC TGGCCCGGCC CTGTCACGGG AACTGTGACC CGACCCAGCC GGAGGCGGGC 
AGTGGGGAGA CGGTGGTGAC CTTCGGGGAC CGGGCCCGGC ACAGGCAGAG CCAGCGGCGC 
AGCCGGCACC GCCGCGTCAG GACCGAGGGC AGGGAGCCCT CCTCCACCTC CAGAAGCAGG 
TCTGCCAGCC AGGAGCGGAG TCTGGACGAA GCTGTGCCCA GCGAGGGGGA GAGGGACCGT 
GAGCCCAGGG CTGGCCATGG GGGCAAAGAA CCCATGATCC AGGAGGAAGA GCGAGAGCGA 
TGCCAGGACT TACGGAGGAC CAACAGTCTG ATGGTGCCCA GAGGCTCCGG GCTGGCAGGA 
GCCCTGGATG AGGCCGACAC ACCCCTTGTC CCACCCCAGC CGGAGCTGGA AGTGGGGAAG 
GACGCAGCCC TGACGGAGCA GGAGCCGGAG GGCAGCAGTG AGCAGGCTCT GCTGGGGGAC 
GTGAAGCTGG ACATAGGCCG GGTCAGCCAG AGCGAGCCCG ACCTCTCCTG CGTCACGGCC 
AACACGGACA AGGCCACCAC CGAGAGCACC AGCGTCACTG TCACCATCCC CGACGTGGGC 
CCCTTGGTGG ACTCAACCGT GGTGCACATC AGCAACAAGA CTGATGGAGA AGCCAGTCCC 
TTGAAAGAGG CAGAGATCAG AGAGGATGAG GAGGAGGTGG AGAAGAAGCA GCAGAAGAAG 
GAGAAGCGTG AGACGGGCAA GGCCATGGTG CCCCACAGCT CGATGTTCAT CTTCAGCACC 
ACCAACCCGA TCCGCAGGGC CTGCCACTAC ATCGTGAACC TGCGCTACTT CGAGATGTGC 
ATCCTCCTGG TGATCGCAGC CAGCAGCATC GCCCTGGCGG CCGAGGACCC CGTCCTGACC 
AACTCAGAGC GCAACAAAGT GCTGAGGTAT TTTGACTATG TGTTCACTGG CGTGTTCACC 
TTTGAGATGG TTATAAAGAT GATAGACCAG GGCTTGATTC TGCAGGACGG CTCCTACTTC 
CGAGACCTGT GGAACATCCT GGACTTCGTG GTGGTGGTTG GCGCATTGGT GGCCTTTGCT 
CTGGCAACTA ACAAGGGCCG GGATATTAAG ACCATCAAGT CCCTGAGGGT TCTCCGTGTC 
CTGAGGCCGC TGAAGACCAT CAAGCGCCTG CCCAAGCTCA AGGCTGTCTT TGACTGTGTG 
GTCACCTCCT TGAAAAACGT CTTCAACATA CTCATTGTCT ACAAGCTCTT CATGTTCATC 
TTTGCTGTCA TCGCAGTTCA GCTTTTCAAG GGCAAGTTCT TCTACTGCAC GGACAGTTCC 
AAGGACACAG AGAAGGAGTG CATAGGCAAC TATGTAGATC ACGAGAAAAA CAAGATGGAG 
GTGAAAGGCC GAGAGTGGAA GCGCCATGAA TTCCATTATG ACAACATCAT CTGGGCTCTG 
CTGACCCTCT TCACTGTCTC CACAGGGGAA GGGTGGCCTC AGGTCTTGCA GCACTCGGTC 
GATGTGACAG AGGAAGACCG AGGCCCAAGC CGCAGCAACC GGATGGAGAT GTCCATCTTT 
TATGTGGTCT ACTTTGTGGT CTTCCCCTTC TTCTTTGTCA ACATCTTTGT GGCTCTCATC 
ATCATCACCT TCCAGGAGCA AGGGGACAAG ATGATGGAAG AGTGCAGCCT GGAGAAGAAT 
GAGAGGGCAT GCATCGACTT CGCCATCAGC GCCAAACCGC TCACCCGCTA CATGCCCCAG 
AACAGGCACA CCTTCCAGTA CCGCGTCTGG CACTTCGTGG TGTCTCCATC CTTTGAGTAC 
ACCATCATGG CCATGATCGC CCTGAACACC GTCGTGCTGA TGATGAAGTA CTACTCTGCT 
CCCTGTACCT ATGAGCTGGC CCTGAAGTAC CTAAATATCG CCTTCACCAT GGTGTTTTCC 
CTGGAATGTG TCCTGAAGAT CATTGCATTT GGCTTCTTGA ACTATTTCCG AGACACCTGG 
AATATCTTTG ACTTCATCAC TGTGATTGGC AGTATCACAG AAATTATTCT GACGGACAGC 
AAGCTGGTGA ACACTAGTGG CTTCAACATG AGCTTTCTGA AGCTCTTCCG AGCGGCCCGC 
CTCATCAAGC TGCTGCGTCA GGGCTACACC ATCCGCATTT TACTGTGGAC CTTCGTGCAG 
TCCTTTAAGG CCCTTCCCTA TGTCTGCCTT CTGATCGCCA TGCTTTTCTT CATCTATGCC 
ATCATTGGGA TGCAGGTCTT TGGAAACATA AAGTTAGATG AGGAGAGTCA CATCAACCGG 
CACAACAACT TCCGGAGTTT CTTTGGGTCC CTCATGCTGC TCTTCAGGAG TGCCACGGGT 
GAGGCCTGGC AGGAGATAAT GCTGTCGTGT CTCGGGGAGA AGGGCTGTGA GCCCGACACC 
ACTGCGCCGT CGGGGCAGAG CGAGAGCGAG CGCTGTGGCA CCGACCTGGC CTACGTGTAC 
TTCGTCTCCT TCATCTTCTT CTGCTCCTTC CTGATGCTCA ACCTGTTCGT GGCTGTCATC 
ATGGACAACT TTGAGTACCT GACTCGGGAC TCGTCCATCC TGGGGCCTCA CCATCTGGAC 
GAGTTTGTCC GAGTCTGGGC AGAGTATGAC AGAGCAGCAT GTGGCCGCAT CCATTACACT 
GAGATGTATG AAATGCTGAC TCTCATGTCA CCGCCGCTAG GCCTCGGCAA GAGATGTCCC 
TCCAAGGTGG CATATAAGAG GTTGGTCTTG ATGAACATGC CAGTGGCCGA GGACATGACC 
GTCCACTTCA CTTCCACGCT GATGGCTCTA ATCCGGACAG CTCTGGACAT TAAAATCGCC 
AAAGGCGGTG CAGACAGGCA GCAGCTAGAC TCAGAGCTCC AGAAGGAGAC CCTGGCCATC 
TGGCCTCACC TGTCCCAGAA GATGCTGGAT CTGCTCGTGC CCATGCCCAA AGCCTCTGAC 
CTGACTGTGG GCAAAATCTA TGCAGCAATG ATGATCATGG ACTACTACAA ACAGAGCAAG 
GTGAAGAAGC AGAGGCAGCA GCTGGAGGAA CAGAAAAACG CACCCATGTT CCAGCGCATG 
GAGCCCTCGT CTCTGCCTCA GGAGATCATC GCTAATGCCA AAGCCCTGCC TTATCTCCAG 
CAGGACCCCG TTCCAGGCCT GAGTGGCCGG AGTGCATACC CTTCGATGAG TCCACTCTCC 
CCGCAGGAAA TATTCCAGTT GGCCTGTATG GACCCAGCTG ATGATGGACA GTTCCAGGAA 
CGACAATCTC TGGAGCCAGA GGTTAGTGAA TTAAAAAGCG TGCAGCCCTC TAACCATGGC 
ATCTACCTTC CTTCGGACAC CCAGGAGCAT GCGGGCTCTG GGAAGGCATC CTCTATGCCA 
CGCCTGACTG TGGATCCCCA GGTGGTGACA GACCCCAGCT CCATGAGACG TTCATTTTCC 
ACCATTCGCG ATAAGCGTTC AAATTCCTCG TGGCTGGAGG AATTCTCCAT GGAGCGAAGC 
AGCGAAAACA CCTACAAGTC CCGGCGCCGG AGTTACCACT CCTCCCTGAG GCTGTCAGCC 
CACCGTCTGA ACTCGGACTC AGGCCACAAG TCCGACACCC ACCGCTCAGG GGGCAGAGAG 
CGGGGACGAT CGAAAGAGCG AAAGCATCTT CTCTCTCCTG ACGTCTCCCG CTGCAACTCC 
GAGGAGCGAG GGACCCAGGC CGACTGGGAG TCCCCAGAGC GCCATCAGTC CCGGTCTCCC 
AGTGAAGGCA GGTCACAGAC CCCCAACAGA CAGGGCACGG GGTCCCTGAG TGAGAGCTCC 
ATCCCCTCTG TCTCCGACTC CAGCACCCCA CGACGAAGTC GGCGGCAGCT CCCCCCAGTC 
CCTCCCAAGC CTCGGCCCCT CCTTTCCTAC AGCTCTCTGA TCAGACACGC TGGGAGCATC 
TCCCCACCAG CTGATGGGAG CGAGGGTGGC TCCCCACTGA CCTCCCAAGC TCTGGAGAGC 
AACGACGCCG GCCTGCCCGA GTCTTCCAAC CCCCGGCAGG GCCACCCCTC CCCACAGCGC 
TACATCTCCG AGCCCTACCT GGCCCTGCAC GAAGACTCCC ACGCCTCGGA CTGCGGCGAG 
GAGGAGACGC TCACCTTTGA AGCAGCCGTG GCCACCAGCC TGGGCCGGTC CAACACCATC 
GGCTCGGCCC CGCCCCTGCG GCACAGCTGG CAGATGCCCA ATGGGCACTA TCGGCGGCGG 
AGGCGCGGGG GGCCTGGACC GGGCACGACG TGCGGAGCCG TCAGCGACCT CCTGAGTGAC 
ACGGAAGAAG ACGACAAATG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1E ( XM_015475248.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330734.1 Bos taurus calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X3, mRNA.

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CloneID	OBa93038
Clone ID Related Accession (Same CDS sequence)	XM_015475248.1
Accession Version	XM_015475248.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6879bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104440.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGCT TCGGGGAGGC GGTGGTCGGC AGGCCGGGGT CGGGCGATGG AGACTCGGAC 
CAGAGCAGGA ACCGGCAAGG AACCCCCGTG CCGGCCTCCG GGCCGGCGGC CGCCTACAAG 
CAGTCGAAAG CGCAGAGGGC GCGGACTATG GCTTTGTACA ACCCTATTCC CGTCCGGCAG 
AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA 
TACGCCAAGA AGCTCATCGA TTGGCCGCCG TTTGAGTACA TGATCTTGGC CACCATCATT 
GCCAATTGCA TCGTCTTGGC CCTGGAGCAA CATCTTCCTG AGGATGACAA GACCCCAATG 
TCCCGCAGAC TGGAGAAGAC AGAACCCTAT TTCATTGGGA TCTTTTGCTT TGAAGCTGGG 
ATCAAAATCG TGGCCCTGGG GTTCATCTTC CATAAGGGAT CATACCTCCG CAATGGTTGG 
AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CTACTGCGGG AACCCACTTC 
AACACCCATG TGGACCTGAG GACCCTCCGG GCCGTGCGTG TCCTGAGGCC CCTGAAGCTC 
GTGTCGGGGA TACCTAGCCT ACAGATTGTG TTGAAATCCA TCATGAAGGC CATGGTGCCT 
CTCCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATCCTGA TGTTTGCTAT CATCGGCTTG 
GAGTTCTACA GTGGAAAGTT GCACCGAGCG TGCTTCATGA ACAATTCAGG TATTCTAGAA 
GGATTTGACC CCCCTCACCC ATGTGGCGTG CAGGGCTGCC CAGCTGGTTA TGAATGCCGG 
GACTGGATCG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCCGTGCTG 
ACCGTCTTCC AGTGTATCAC CATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT 
GCCTTAGGAG CCACCTGGAA TTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC 
TTTGTTCTCA ACCTTGTCCT GGGAGTGCTT TCTGGGGAAT TTGCCAAAGA GAGAGAGAGA 
GTGGAGAACC GGAGAGCCTT CATGAAACTG CGGCGCCAGC AGCAGATCGA GCGTGAGCTT 
AACGGGTACC GCGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTTGCTGA AGAAAACAAA 
AATGCTGGAA CGTCAGCCTT AGAAGTGCTT CGGAGGGCAA CCATCAAAAG GAGCCGGACC 
GAGGCCATGG CACGGGACTC CAGCGATGAG CACTGCGTGG ACATCTCCTC TGTGGGCACA 
CCTCTCGCCC GAGCCAGCAT CAAGAGCGCC AAGGTGGACG GGGCCTCCTA TTTCCGGCAC 
AAGGAGCGAC TTTTGCGGAT CTCTATCCGC CACATGGTTA AGTCCCAGGT GTTCTACTGG 
ATCGTGCTGA GCCTGGTGGC TCTCAACACC GCCTGTGTGG CCATCGTCCA CCACAACCAG 
CCCCAGTGGC TCACCCACCT CCTCTACTAT GCAGAGTTTC TGTTTCTGGG GCTCTTCCTT 
CTAGAGATGT CCCTGAAGAT GTACGGCATG GGGCCTCGCC TTTATTTTCA CTCTTCCTTC 
AACTGCTTTG ACTTTGGGGT CACAGTGGGC AGCATCTTTG AAGTAGTCTG GGCCATCTTC 
AGACCTGGTA CATCTTTCGG AATCAGTGTC CTGCGAGCTC TCCGGCTTCT AAGAATATTT 
AAAATAACCA AGTATTGGGC ATCCCTCCGG AATTTGGTGG TCTCCTTGAT GAGTTCCATG 
AAGTCTATCA TCAGTTTACT CTTCCTCCTT TTCCTCTTCA TTGTTGTCTT TGCTCTGCTA 
GGAATGCAGC TGTTTGGGGG CAGGTTCAAC TTTAATGATG GGACTCCTTC CGCAAACTTT 
GACACCTTCC CTGCAGCCAT CATGACTGTA TTCCAGATCC TGACGGGTGA GGACTGGAAT 
GAGGTGATGT ACAACGGGAT CCGCTCCCAG GGTGGGGTCA GCTCGGGCAT GTGGTCTGCG 
ATCTATTTCA TTGTGCTTAC CTTGTTTGGA AACTACACAC TGCTGAATGT GTTCTTGGCC 
ATCGCTGTGG ACAATCTGGC CAACGCCCAG GAGCTGACCA AGGATGAACA AGAAGAAGAG 
GAGGCCTTCA ACCAGAAGCA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA 
CCCAACATGC CTTCCATCGA GAGGGAGCGG AGGCGCCGGC ATCACATGTC CGTGTGGGAG 
CAGCGAACCA GCCAGTTGAG GAGGCACATG CAGATGTCCA GCCAGGAGGC CCTCAACAAG 
GAGGAGGCCC CGCCCATGAA CCCCCTCAAC CCCCTCAACC CGCTGAGCCC CCTCAACCCG 
CTCAGCGCGC ACCCCAGCCT CTACCGGAGA CCCCGGGCCA TCGAGGGGCT AGCCCTGGGC 
CTGGGGCTGG AGAAGTGTGA GGAAGAGCGC GTCAGCCGTG GGGGGTCCCT CAAAGGGGAT 
GGAGGGGACT TGGCGAGCGC CCTGGACAGC CAGAGGAGCC CCCTGGCCCT GGGCAAACGG 
GAGCCACCCT GGCTGGCCCG GCCCTGTCAC GGGAACTGTG ACCCGACCCA GCCGGAGGCG 
GGCAGTGGGG AGACGGTGGT GACCTTCGGG GACCGGGCCC GGCACAGGCA GAGCCAGCGG 
CGCAGCCGGC ACCGCCGCGT CAGGACCGAG GGCAGGGAGC CCTCCTCCAC CTCCAGAAGC 
AGGTCTGCCA GCCAGGAGCG GAGTCTGGAC GAAGCTGTGC CCAGCGAGGG GGAGAGGGAC 
CGTGAGCCCA GGGCTGGCCA TGGGGGCAAA GAACCCATGA TCCAGGAGGA AGAGCGAGAG 
CGATGCCAGG ACTTACGGAG GACCAACAGT CTGATGGTGC CCAGAGGCTC CGGGCTGGCA 
GGAGCCCTGG ATGAGGCCGA CACACCCCTT GTCCCACCCC AGCCGGAGCT GGAAGTGGGG 
AAGGACGCAG CCCTGACGGA GCAGGAGCCG GAGGGCAGCA GTGAGCAGGC TCTGCTGGGG 
GACGTGAAGC TGGACATAGG CCGGGTCAGC CAGAGCGAGC CCGACCTCTC CTGCGTCACG 
GCCAACACGG ACAAGGCCAC CACCGAGAGC ACCAGCGTCA CTGTCACCAT CCCCGACGTG 
GGCCCCTTGG TGGACTCAAC CGTGGTGCAC ATCAGCAACA AGACTGATGG AGAAGCCAGT 
CCCTTGAAAG AGGCAGAGAT CAGAGAGGAT GAGGAGGAGG TGGAGAAGAA GCAGCAGAAG 
AAGGAGAAGC GTGAGACGGG CAAGGCCATG GTGCCCCACA GCTCGATGTT CATCTTCAGC 
ACCACCAACC CGATCCGCAG GGCCTGCCAC TACATCGTGA ACCTGCGCTA CTTCGAGATG 
TGCATCCTCC TGGTGATCGC AGCCAGCAGC ATCGCCCTGG CGGCCGAGGA CCCCGTCCTG 
ACCAACTCAG AGCGCAACAA AGTGCTGAGG TATTTTGACT ATGTGTTCAC TGGCGTGTTC 
ACCTTTGAGA TGGTTATAAA GATGATAGAC CAGGGCTTGA TTCTGCAGGA CGGCTCCTAC 
TTCCGAGACC TGTGGAACAT CCTGGACTTC GTGGTGGTGG TTGGCGCATT GGTGGCCTTT 
GCTCTGGCGA ATGCTTTGGG AACTAACAAG GGCCGGGATA TTAAGACCAT CAAGTCCCTG 
AGGGTTCTCC GTGTCCTGAG GCCGCTGAAG ACCATCAAGC GCCTGCCCAA GCTCAAGGCT 
GTCTTTGACT GTGTGGTCAC CTCCTTGAAA AACGTCTTCA ACATACTCAT TGTCTACAAG 
CTCTTCATGT TCATCTTTGC TGTCATCGCA GTTCAGCTTT TCAAGGGCAA GTTCTTCTAC 
TGCACGGACA GTTCCAAGGA CACAGAGAAG GAGTGCATAG GCAACTATGT AGATCACGAG 
AAAAACAAGA TGGAGGTGAA AGGCCGAGAG TGGAAGCGCC ATGAATTCCA TTATGACAAC 
ATCATCTGGG CTCTGCTGAC CCTCTTCACT GTCTCCACAG GGGAAGGGTG GCCTCAGGTC 
TTGCAGCACT CGGTCGATGT GACAGAGGAA GACCGAGGCC CAAGCCGCAG CAACCGGATG 
GAGATGTCCA TCTTTTATGT GGTCTACTTT GTGGTCTTCC CCTTCTTCTT TGTCAACATC 
TTTGTGGCTC TCATCATCAT CACCTTCCAG GAGCAAGGGG ACAAGATGAT GGAAGAGTGC 
AGCCTGGAGA AGAATGAGAG GGCATGCATC GACTTCGCCA TCAGCGCCAA ACCGCTCACC 
CGCTACATGC CCCAGAACAG GCACACCTTC CAGTACCGCG TCTGGCACTT CGTGGTGTCT 
CCATCCTTTG AGTACACCAT CATGGCCATG ATCGCCCTGA ACACCGTCGT GCTGATGATG 
AAGTACTACT CTGCTCCCTG TACCTATGAG CTGGCCCTGA AGTACCTAAA TATCGCCTTC 
ACCATGGTGT TTTCCCTGGA ATGTGTCCTG AAGATCATTG CATTTGGCTT CTTGAACTAT 
TTCCGAGACA CCTGGAATAT CTTTGACTTC ATCACTGTGA TTGGCAGTAT CACAGAAATT 
ATTCTGACGG ACAGCAAGCT GGTGAACACT AGTGGCTTCA ACATGAGCTT TCTGAAGCTC 
TTCCGAGCGG CCCGCCTCAT CAAGCTGCTG CGTCAGGGCT ACACCATCCG CATTTTACTG 
TGGACCTTCG TGCAGTCCTT TAAGGCCCTT CCCTATGTCT GCCTTCTGAT CGCCATGCTT 
TTCTTCATCT ATGCCATCAT TGGGATGCAG GTCTTTGGAA ACATAAAGTT AGATGAGGAG 
AGTCACATCA ACCGGCACAA CAACTTCCGG AGTTTCTTTG GGTCCCTCAT GCTGCTCTTC 
AGGAGTGCCA CGGGTGAGGC CTGGCAGGAG ATAATGCTGT CGTGTCTCGG GGAGAAGGGC 
TGTGAGCCCG ACACCACTGC GCCGTCGGGG CAGAGCGAGA GCGAGCGCTG TGGCACCGAC 
CTGGCCTACG TGTACTTCGT CTCCTTCATC TTCTTCTGCT CCTTCCTGAT GCTCAACCTG 
TTCGTGGCTG TCATCATGGA CAACTTTGAG TACCTGACTC GGGACTCGTC CATCCTGGGG 
CCTCACCATC TGGACGAGTT TGTCCGAGTC TGGGCAGAGT ATGACAGAGC AGCATGTGGC 
CGCATCCATT ACACTGAGAT GTATGAAATG CTGACTCTCA TGTCACCGCC GCTAGGCCTC 
GGCAAGAGAT GTCCCTCCAA GGTGGCATAT AAGAGGTTGG TCTTGATGAA CATGCCAGTG 
GCCGAGGACA TGACCGTCCA CTTCACTTCC ACGCTGATGG CTCTAATCCG GACAGCTCTG 
GACATTAAAA TCGCCAAAGG CGGTGCAGAC AGGCAGCAGC TAGACTCAGA GCTCCAGAAG 
GAGACCCTGG CCATCTGGCC TCACCTGTCC CAGAAGATGC TGGATCTGCT CGTGCCCATG 
CCCAAAGCCT CTGACCTGAC TGTGGGCAAA ATCTATGCAG CAATGATGAT CATGGACTAC 
TACAAACAGA GCAAGGTGAA GAAGCAGAGG CAGCAGCTGG AGGAACAGAA AAACGCACCC 
ATGTTCCAGC GCATGGAGCC CTCGTCTCTG CCTCAGGAGA TCATCGCTAA TGCCAAAGCC 
CTGCCTTATC TCCAGCAGGA CCCCGTTCCA GGCCTGAGTG GCCGGAGTGC ATACCCTTCG 
ATGAGTCCAC TCTCCCCGCA GGAAATATTC CAGTTGGCCT GTATGGACCC AGCTGATGAT 
GGACAGTTCC AGGAACGACA ATCTCTGGAG CCAGAGGTTA GTGAATTAAA AAGCGTGCAG 
CCCTCTAACC ATGGCATCTA CCTTCCTTCG GACACCCAGG AGCATGCGGG CTCTGGGAAG 
GCATCCTCTA TGCCACGCCT GACTGTGGAT CCCCAGGTGG TGACAGACCC CAGCTCCATG 
AGACGTTCAT TTTCCACCAT TCGCGATAAG CGTTCAAATT CCTCGTGGCT GGAGGAATTC 
TCCATGGAGC GAAGCAGCGA AAACACCTAC AAGTCCCGGC GCCGGAGTTA CCACTCCTCC 
CTGAGGCTGT CAGCCCACCG TCTGAACTCG GACTCAGGCC ACAAGTCCGA CACCCACCGC 
TCAGGGGGCA GAGAGCGGGG ACGATCGAAA GAGCGAAAGC ATCTTCTCTC TCCTGACGTC 
TCCCGCTGCA ACTCCGAGGA GCGAGGGACC CAGGCCGACT GGGAGTCCCC AGAGCGCCAT 
CAGTCCCGGT CTCCCAGTGA AGGCAGGTCA CAGACCCCCA ACAGACAGGG CACGGGGTCC 
CTGAGTGAGA GCTCCATCCC CTCTGTCTCC GACTCCAGCA CCCCACGACG AAGTCGGCGG 
CAGCTCCCCC CAGTCCCTCC CAAGCCTCGG CCCCTCCTTT CCTACAGCTC TCTGATCAGA 
CACGCTGGGA GCATCTCCCC ACCAGCTGAT GGGAGCGAGG GTGGCTCCCC ACTGACCTCC 
CAAGCTCTGG AGAGCAACGA CGCCGGCCTG CCCGAGTCTT CCAACCCCCG GCAGGGCCAC 
CCCTCCCCAC AGCGCTACAT CTCCGAGCCC TACCTGGCCC TGCACGAAGA CTCCCACGCC 
TCGGACTGCG GCGAGGAGGA GACGCTCACC TTTGAAGCAG CCGTGGCCAC CAGCCTGGGC 
CGGTCCAACA CCATCGGCTC GGCCCCGCCC CTGCGGCACA GCTGGCAGAT GCCCAATGGG 
CACTATCGGC GGCGGAGGCG CGGGGGGCCT GGACCGGGCA CGACGTGCGG AGCCGTCAGC 
GACCTCCTGA GTGACACGGA AGAAGACGAC AAATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330734.1
CDS	163..7041
Translation	MARFGEAVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECRDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNAGTSALEVLRRATIKRSRTEAMARDSSDEHCVDISSVGTPLARASIKSAKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSLVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLSAHPSLYRRPRAIEGLALGLGLEKCEEERVSRGGSLKGDGGDLASALDSQRSPLALGKREPPWLARPCHGNCDPTQPEAGSGETVVTFGDRARHRQSQRRSRHRRVRTEGREPSSTSRSRSASQERSLDEAVPSEGERDREPRAGHGGKEPMIQEEERERCQDLRRTNSLMVPRGSGLAGALDEADTPLVPPQPELEVGKDAALTEQEPEGSSEQALLGDVKLDIGRVSQSEPDLSCVTANTDKATTESTSVTVTIPDVGPLVDSTVVHISNKTDGEASPLKEAEIREDEEEVEKKQQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKIIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQSESERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVPGLSGRSAYPSMSPLSPQEIFQLACMDPADDGQFQERQSLEPEVSELKSVQPSNHGIYLPSDTQEHAGSGKASSMPRLTVDPQVVTDPSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERHQSRSPSEGRSQTPNRQGTGSLSESSIPSVSDSSTPRRSRRQLPPVPPKPRPLLSYSSLIRHAGSISPPADGSEGGSPLTSQALESNDAGLPESSNPRQGHPSPQRYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGTTCGAVSDLLSDTEEDDKC

Target ORF information:

RefSeq Version	XM_015475248.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015475248.1

ORF Insert Sequence:

ATGGCTCGCT TCGGGGAGGC GGTGGTCGGC AGGCCGGGGT CGGGCGATGG AGACTCGGAC 
CAGAGCAGGA ACCGGCAAGG AACCCCCGTG CCGGCCTCCG GGCCGGCGGC CGCCTACAAG 
CAGTCGAAAG CGCAGAGGGC GCGGACTATG GCTTTGTACA ACCCTATTCC CGTCCGGCAG 
AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA 
TACGCCAAGA AGCTCATCGA TTGGCCGCCG TTTGAGTACA TGATCTTGGC CACCATCATT 
GCCAATTGCA TCGTCTTGGC CCTGGAGCAA CATCTTCCTG AGGATGACAA GACCCCAATG 
TCCCGCAGAC TGGAGAAGAC AGAACCCTAT TTCATTGGGA TCTTTTGCTT TGAAGCTGGG 
ATCAAAATCG TGGCCCTGGG GTTCATCTTC CATAAGGGAT CATACCTCCG CAATGGTTGG 
AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CTACTGCGGG AACCCACTTC 
AACACCCATG TGGACCTGAG GACCCTCCGG GCCGTGCGTG TCCTGAGGCC CCTGAAGCTC 
GTGTCGGGGA TACCTAGCCT ACAGATTGTG TTGAAATCCA TCATGAAGGC CATGGTGCCT 
CTCCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATCCTGA TGTTTGCTAT CATCGGCTTG 
GAGTTCTACA GTGGAAAGTT GCACCGAGCG TGCTTCATGA ACAATTCAGG TATTCTAGAA 
GGATTTGACC CCCCTCACCC ATGTGGCGTG CAGGGCTGCC CAGCTGGTTA TGAATGCCGG 
GACTGGATCG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCCGTGCTG 
ACCGTCTTCC AGTGTATCAC CATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT 
GCCTTAGGAG CCACCTGGAA TTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC 
TTTGTTCTCA ACCTTGTCCT GGGAGTGCTT TCTGGGGAAT TTGCCAAAGA GAGAGAGAGA 
GTGGAGAACC GGAGAGCCTT CATGAAACTG CGGCGCCAGC AGCAGATCGA GCGTGAGCTT 
AACGGGTACC GCGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTTGCTGA AGAAAACAAA 
AATGCTGGAA CGTCAGCCTT AGAAGTGCTT CGGAGGGCAA CCATCAAAAG GAGCCGGACC 
GAGGCCATGG CACGGGACTC CAGCGATGAG CACTGCGTGG ACATCTCCTC TGTGGGCACA 
CCTCTCGCCC GAGCCAGCAT CAAGAGCGCC AAGGTGGACG GGGCCTCCTA TTTCCGGCAC 
AAGGAGCGAC TTTTGCGGAT CTCTATCCGC CACATGGTTA AGTCCCAGGT GTTCTACTGG 
ATCGTGCTGA GCCTGGTGGC TCTCAACACC GCCTGTGTGG CCATCGTCCA CCACAACCAG 
CCCCAGTGGC TCACCCACCT CCTCTACTAT GCAGAGTTTC TGTTTCTGGG GCTCTTCCTT 
CTAGAGATGT CCCTGAAGAT GTACGGCATG GGGCCTCGCC TTTATTTTCA CTCTTCCTTC 
AACTGCTTTG ACTTTGGGGT CACAGTGGGC AGCATCTTTG AAGTAGTCTG GGCCATCTTC 
AGACCTGGTA CATCTTTCGG AATCAGTGTC CTGCGAGCTC TCCGGCTTCT AAGAATATTT 
AAAATAACCA AGTATTGGGC ATCCCTCCGG AATTTGGTGG TCTCCTTGAT GAGTTCCATG 
AAGTCTATCA TCAGTTTACT CTTCCTCCTT TTCCTCTTCA TTGTTGTCTT TGCTCTGCTA 
GGAATGCAGC TGTTTGGGGG CAGGTTCAAC TTTAATGATG GGACTCCTTC CGCAAACTTT 
GACACCTTCC CTGCAGCCAT CATGACTGTA TTCCAGATCC TGACGGGTGA GGACTGGAAT 
GAGGTGATGT ACAACGGGAT CCGCTCCCAG GGTGGGGTCA GCTCGGGCAT GTGGTCTGCG 
ATCTATTTCA TTGTGCTTAC CTTGTTTGGA AACTACACAC TGCTGAATGT GTTCTTGGCC 
ATCGCTGTGG ACAATCTGGC CAACGCCCAG GAGCTGACCA AGGATGAACA AGAAGAAGAG 
GAGGCCTTCA ACCAGAAGCA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA 
CCCAACATGC CTTCCATCGA GAGGGAGCGG AGGCGCCGGC ATCACATGTC CGTGTGGGAG 
CAGCGAACCA GCCAGTTGAG GAGGCACATG CAGATGTCCA GCCAGGAGGC CCTCAACAAG 
GAGGAGGCCC CGCCCATGAA CCCCCTCAAC CCCCTCAACC CGCTGAGCCC CCTCAACCCG 
CTCAGCGCGC ACCCCAGCCT CTACCGGAGA CCCCGGGCCA TCGAGGGGCT AGCCCTGGGC 
CTGGGGCTGG AGAAGTGTGA GGAAGAGCGC GTCAGCCGTG GGGGGTCCCT CAAAGGGGAT 
GGAGGGGACT TGGCGAGCGC CCTGGACAGC CAGAGGAGCC CCCTGGCCCT GGGCAAACGG 
GAGCCACCCT GGCTGGCCCG GCCCTGTCAC GGGAACTGTG ACCCGACCCA GCCGGAGGCG 
GGCAGTGGGG AGACGGTGGT GACCTTCGGG GACCGGGCCC GGCACAGGCA GAGCCAGCGG 
CGCAGCCGGC ACCGCCGCGT CAGGACCGAG GGCAGGGAGC CCTCCTCCAC CTCCAGAAGC 
AGGTCTGCCA GCCAGGAGCG GAGTCTGGAC GAAGCTGTGC CCAGCGAGGG GGAGAGGGAC 
CGTGAGCCCA GGGCTGGCCA TGGGGGCAAA GAACCCATGA TCCAGGAGGA AGAGCGAGAG 
CGATGCCAGG ACTTACGGAG GACCAACAGT CTGATGGTGC CCAGAGGCTC CGGGCTGGCA 
GGAGCCCTGG ATGAGGCCGA CACACCCCTT GTCCCACCCC AGCCGGAGCT GGAAGTGGGG 
AAGGACGCAG CCCTGACGGA GCAGGAGCCG GAGGGCAGCA GTGAGCAGGC TCTGCTGGGG 
GACGTGAAGC TGGACATAGG CCGGGTCAGC CAGAGCGAGC CCGACCTCTC CTGCGTCACG 
GCCAACACGG ACAAGGCCAC CACCGAGAGC ACCAGCGTCA CTGTCACCAT CCCCGACGTG 
GGCCCCTTGG TGGACTCAAC CGTGGTGCAC ATCAGCAACA AGACTGATGG AGAAGCCAGT 
CCCTTGAAAG AGGCAGAGAT CAGAGAGGAT GAGGAGGAGG TGGAGAAGAA GCAGCAGAAG 
AAGGAGAAGC GTGAGACGGG CAAGGCCATG GTGCCCCACA GCTCGATGTT CATCTTCAGC 
ACCACCAACC CGATCCGCAG GGCCTGCCAC TACATCGTGA ACCTGCGCTA CTTCGAGATG 
TGCATCCTCC TGGTGATCGC AGCCAGCAGC ATCGCCCTGG CGGCCGAGGA CCCCGTCCTG 
ACCAACTCAG AGCGCAACAA AGTGCTGAGG TATTTTGACT ATGTGTTCAC TGGCGTGTTC 
ACCTTTGAGA TGGTTATAAA GATGATAGAC CAGGGCTTGA TTCTGCAGGA CGGCTCCTAC 
TTCCGAGACC TGTGGAACAT CCTGGACTTC GTGGTGGTGG TTGGCGCATT GGTGGCCTTT 
GCTCTGGCGA ATGCTTTGGG AACTAACAAG GGCCGGGATA TTAAGACCAT CAAGTCCCTG 
AGGGTTCTCC GTGTCCTGAG GCCGCTGAAG ACCATCAAGC GCCTGCCCAA GCTCAAGGCT 
GTCTTTGACT GTGTGGTCAC CTCCTTGAAA AACGTCTTCA ACATACTCAT TGTCTACAAG 
CTCTTCATGT TCATCTTTGC TGTCATCGCA GTTCAGCTTT TCAAGGGCAA GTTCTTCTAC 
TGCACGGACA GTTCCAAGGA CACAGAGAAG GAGTGCATAG GCAACTATGT AGATCACGAG 
AAAAACAAGA TGGAGGTGAA AGGCCGAGAG TGGAAGCGCC ATGAATTCCA TTATGACAAC 
ATCATCTGGG CTCTGCTGAC CCTCTTCACT GTCTCCACAG GGGAAGGGTG GCCTCAGGTC 
TTGCAGCACT CGGTCGATGT GACAGAGGAA GACCGAGGCC CAAGCCGCAG CAACCGGATG 
GAGATGTCCA TCTTTTATGT GGTCTACTTT GTGGTCTTCC CCTTCTTCTT TGTCAACATC 
TTTGTGGCTC TCATCATCAT CACCTTCCAG GAGCAAGGGG ACAAGATGAT GGAAGAGTGC 
AGCCTGGAGA AGAATGAGAG GGCATGCATC GACTTCGCCA TCAGCGCCAA ACCGCTCACC 
CGCTACATGC CCCAGAACAG GCACACCTTC CAGTACCGCG TCTGGCACTT CGTGGTGTCT 
CCATCCTTTG AGTACACCAT CATGGCCATG ATCGCCCTGA ACACCGTCGT GCTGATGATG 
AAGTACTACT CTGCTCCCTG TACCTATGAG CTGGCCCTGA AGTACCTAAA TATCGCCTTC 
ACCATGGTGT TTTCCCTGGA ATGTGTCCTG AAGATCATTG CATTTGGCTT CTTGAACTAT 
TTCCGAGACA CCTGGAATAT CTTTGACTTC ATCACTGTGA TTGGCAGTAT CACAGAAATT 
ATTCTGACGG ACAGCAAGCT GGTGAACACT AGTGGCTTCA ACATGAGCTT TCTGAAGCTC 
TTCCGAGCGG CCCGCCTCAT CAAGCTGCTG CGTCAGGGCT ACACCATCCG CATTTTACTG 
TGGACCTTCG TGCAGTCCTT TAAGGCCCTT CCCTATGTCT GCCTTCTGAT CGCCATGCTT 
TTCTTCATCT ATGCCATCAT TGGGATGCAG GTCTTTGGAA ACATAAAGTT AGATGAGGAG 
AGTCACATCA ACCGGCACAA CAACTTCCGG AGTTTCTTTG GGTCCCTCAT GCTGCTCTTC 
AGGAGTGCCA CGGGTGAGGC CTGGCAGGAG ATAATGCTGT CGTGTCTCGG GGAGAAGGGC 
TGTGAGCCCG ACACCACTGC GCCGTCGGGG CAGAGCGAGA GCGAGCGCTG TGGCACCGAC 
CTGGCCTACG TGTACTTCGT CTCCTTCATC TTCTTCTGCT CCTTCCTGAT GCTCAACCTG 
TTCGTGGCTG TCATCATGGA CAACTTTGAG TACCTGACTC GGGACTCGTC CATCCTGGGG 
CCTCACCATC TGGACGAGTT TGTCCGAGTC TGGGCAGAGT ATGACAGAGC AGCATGTGGC 
CGCATCCATT ACACTGAGAT GTATGAAATG CTGACTCTCA TGTCACCGCC GCTAGGCCTC 
GGCAAGAGAT GTCCCTCCAA GGTGGCATAT AAGAGGTTGG TCTTGATGAA CATGCCAGTG 
GCCGAGGACA TGACCGTCCA CTTCACTTCC ACGCTGATGG CTCTAATCCG GACAGCTCTG 
GACATTAAAA TCGCCAAAGG CGGTGCAGAC AGGCAGCAGC TAGACTCAGA GCTCCAGAAG 
GAGACCCTGG CCATCTGGCC TCACCTGTCC CAGAAGATGC TGGATCTGCT CGTGCCCATG 
CCCAAAGCCT CTGACCTGAC TGTGGGCAAA ATCTATGCAG CAATGATGAT CATGGACTAC 
TACAAACAGA GCAAGGTGAA GAAGCAGAGG CAGCAGCTGG AGGAACAGAA AAACGCACCC 
ATGTTCCAGC GCATGGAGCC CTCGTCTCTG CCTCAGGAGA TCATCGCTAA TGCCAAAGCC 
CTGCCTTATC TCCAGCAGGA CCCCGTTCCA GGCCTGAGTG GCCGGAGTGC ATACCCTTCG 
ATGAGTCCAC TCTCCCCGCA GGAAATATTC CAGTTGGCCT GTATGGACCC AGCTGATGAT 
GGACAGTTCC AGGAACGACA ATCTCTGGAG CCAGAGGTTA GTGAATTAAA AAGCGTGCAG 
CCCTCTAACC ATGGCATCTA CCTTCCTTCG GACACCCAGG AGCATGCGGG CTCTGGGAAG 
GCATCCTCTA TGCCACGCCT GACTGTGGAT CCCCAGGTGG TGACAGACCC CAGCTCCATG 
AGACGTTCAT TTTCCACCAT TCGCGATAAG CGTTCAAATT CCTCGTGGCT GGAGGAATTC 
TCCATGGAGC GAAGCAGCGA AAACACCTAC AAGTCCCGGC GCCGGAGTTA CCACTCCTCC 
CTGAGGCTGT CAGCCCACCG TCTGAACTCG GACTCAGGCC ACAAGTCCGA CACCCACCGC 
TCAGGGGGCA GAGAGCGGGG ACGATCGAAA GAGCGAAAGC ATCTTCTCTC TCCTGACGTC 
TCCCGCTGCA ACTCCGAGGA GCGAGGGACC CAGGCCGACT GGGAGTCCCC AGAGCGCCAT 
CAGTCCCGGT CTCCCAGTGA AGGCAGGTCA CAGACCCCCA ACAGACAGGG CACGGGGTCC 
CTGAGTGAGA GCTCCATCCC CTCTGTCTCC GACTCCAGCA CCCCACGACG AAGTCGGCGG 
CAGCTCCCCC CAGTCCCTCC CAAGCCTCGG CCCCTCCTTT CCTACAGCTC TCTGATCAGA 
CACGCTGGGA GCATCTCCCC ACCAGCTGAT GGGAGCGAGG GTGGCTCCCC ACTGACCTCC 
CAAGCTCTGG AGAGCAACGA CGCCGGCCTG CCCGAGTCTT CCAACCCCCG GCAGGGCCAC 
CCCTCCCCAC AGCGCTACAT CTCCGAGCCC TACCTGGCCC TGCACGAAGA CTCCCACGCC 
TCGGACTGCG GCGAGGAGGA GACGCTCACC TTTGAAGCAG CCGTGGCCAC CAGCCTGGGC 
CGGTCCAACA CCATCGGCTC GGCCCCGCCC CTGCGGCACA GCTGGCAGAT GCCCAATGGG 
CACTATCGGC GGCGGAGGCG CGGGGGGCCT GGACCGGGCA CGACGTGCGG AGCCGTCAGC 
GACCTCCTGA GTGACACGGA AGAAGACGAC AAATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1E ( XM_015475249.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330735.1 Bos taurus calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X4, mRNA.

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CloneID	OBa93039
Clone ID Related Accession (Same CDS sequence)	XM_015475249.1
Accession Version	XM_015475249.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104440.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGCT TCGGGGAGGC GGTGGTCGGC AGGCCGGGGT CGGGCGATGG AGACTCGGAC 
CAGAGCAGGA ACCGGCAAGG AACCCCCGTG CCGGCCTCCG GGCCGGCGGC CGCCTACAAG 
CAGTCGAAAG CGCAGAGGGC GCGGACTATG GCTTTGTACA ACCCTATTCC CGTCCGGCAG 
AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA 
TACGCCAAGA AGCTCATCGA TTGGCCGCCG TTTGAGTACA TGATCTTGGC CACCATCATT 
GCCAATTGCA TCGTCTTGGC CCTGGAGCAA CATCTTCCTG AGGATGACAA GACCCCAATG 
TCCCGCAGAC TGGAGAAGAC AGAACCCTAT TTCATTGGGA TCTTTTGCTT TGAAGCTGGG 
ATCAAAATCG TGGCCCTGGG GTTCATCTTC CATAAGGGAT CATACCTCCG CAATGGTTGG 
AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CTACTGCGGG AACCCACTTC 
AACACCCATG TGGACCTGAG GACCCTCCGG GCCGTGCGTG TCCTGAGGCC CCTGAAGCTC 
GTGTCGGGGA TACCTAGCCT ACAGATTGTG TTGAAATCCA TCATGAAGGC CATGGTGCCT 
CTCCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATCCTGA TGTTTGCTAT CATCGGCTTG 
GAGTTCTACA GTGGAAAGTT GCACCGAGCG TGCTTCATGA ACAATTCAGG TATTCTAGAA 
GGATTTGACC CCCCTCACCC ATGTGGCGTG CAGGGCTGCC CAGCTGGTTA TGAATGCCGG 
GACTGGATCG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCCGTGCTG 
ACCGTCTTCC AGTGTATCAC CATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT 
GCCTTAGGAG CCACCTGGAA TTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC 
TTTGTTCTCA ACCTTGTCCT GGGAGTGCTT TCTGGGGAAT TTGCCAAAGA GAGAGAGAGA 
GTGGAGAACC GGAGAGCCTT CATGAAACTG CGGCGCCAGC AGCAGATCGA GCGTGAGCTT 
AACGGGTACC GCGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTTGCTGA AGAAAACAAA 
AATGCTGGAA CGTCAGCCTT AGAAGTGCTT CGGAGGGCAA CCATCAAAAG GAGCCGGACC 
GAGGCCATGG CACGGGACTC CAGCGATGAG CACTGCGTGG ACATCTCCTC TGTGGGCACA 
CCTCTCGCCC GAGCCAGCAT CAAGAGCGCC AAGGTGGACG GGGCCTCCTA TTTCCGGCAC 
AAGGAGCGAC TTTTGCGGAT CTCTATCCGC CACATGGTTA AGTCCCAGGT GTTCTACTGG 
ATCGTGCTGA GCCTGGTGGC TCTCAACACC GCCTGTGTGG CCATCGTCCA CCACAACCAG 
CCCCAGTGGC TCACCCACCT CCTCTACTAT GCAGAGTTTC TGTTTCTGGG GCTCTTCCTT 
CTAGAGATGT CCCTGAAGAT GTACGGCATG GGGCCTCGCC TTTATTTTCA CTCTTCCTTC 
AACTGCTTTG ACTTTGGGGT CACAGTGGGC AGCATCTTTG AAGTAGTCTG GGCCATCTTC 
AGACCTGGTA CATCTTTCGG AATCAGTGTC CTGCGAGCTC TCCGGCTTCT AAGAATATTT 
AAAATAACCA AGTATTGGGC ATCCCTCCGG AATTTGGTGG TCTCCTTGAT GAGTTCCATG 
AAGTCTATCA TCAGTTTACT CTTCCTCCTT TTCCTCTTCA TTGTTGTCTT TGCTCTGCTA 
GGAATGCAGC TGTTTGGGGG CAGGTTCAAC TTTAATGATG GGACTCCTTC CGCAAACTTT 
GACACCTTCC CTGCAGCCAT CATGACTGTA TTCCAGATCC TGACGGGTGA GGACTGGAAT 
GAGGTGATGT ACAACGGGAT CCGCTCCCAG GGTGGGGTCA GCTCGGGCAT GTGGTCTGCG 
ATCTATTTCA TTGTGCTTAC CTTGTTTGGA AACTACACAC TGCTGAATGT GTTCTTGGCC 
ATCGCTGTGG ACAATCTGGC CAACGCCCAG GAGCTGACCA AGGATGAACA AGAAGAAGAG 
GAGGCCTTCA ACCAGAAGCA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA 
CCCAACATGC CTTCCATCGA GAGGGAGCGG AGGCGCCGGC ATCACATGTC CGTGTGGGAG 
CAGCGAACCA GCCAGTTGAG GAGGCACATG CAGATGTCCA GCCAGGAGGC CCTCAACAAG 
GAGGAGGCCC CGCCCATGAA CCCCCTCAAC CCCCTCAACC CGCTGAGCCC CCTCAACCCG 
CTCAGCGCGC ACCCCAGCCT CTACCGGAGA CCCCGGGCCA TCGAGGGGCT AGCCCTGGGC 
CTGGGGCTGG AGAAGTGTGA GGAAGAGCGC GTCAGCCGTG GGGGGTCCCT CAAAGGGGAT 
GGAGGGGACT TGGCGAGCGC CCTGGACAGC CAGAGGAGCC CCCTGGCCCT GGGCAAACGG 
GAGCCACCCT GGCTGGCCCG GCCCTGTCAC GGGAACTGTG ACCCGACCCA GCCGGAGGCG 
GGCAGTGGGG AGACGGTGGT GACCTTCGGG GACCGGGCCC GGCACAGGCA GAGCCAGCGG 
CGCAGCCGGC ACCGCCGCGT CAGGACCGAG GGCAGGGAGC CCTCCTCCAC CTCCAGAAGC 
AGGTCTGCCA GCCAGGAGCG GAGTCTGGAC GAAGCTGTGC CCAGCGAGGG GGAGAGGGAC 
CGTGAGCCCA GGGCTGGCCA TGGGGGCAAA GAACCCATGA TCCAGGAGGA AGAGCGAGAG 
CGATGCCAGG ACTTACGGAG GACCAACAGT CTGATGGTGC CCAGAGGCTC CGGGCTGGCA 
GGAGCCCTGG ATGAGGCCGA CACACCCCTT GTCCCACCCC AGCCGGAGCT GGAAGTGGGG 
AAGGACGCAG CCCTGACGGA GCAGGAGCCG GAGGGCAGCA GTGAGCAGGC TCTGCTGGGG 
GACGTGAAGC TGGACATAGG CCGGGTCAGC CAGAGCGAGC CCGACCTCTC CTGCGTCACG 
GCCAACACGG ACAAGGCCAC CACCGAGAGC ACCAGCGTCA CTGTCACCAT CCCCGACGTG 
GGCCCCTTGG TGGACTCAAC CGTGGTGCAC ATCAGCAACA AGACTGATGG AGAAGCCAGT 
CCCTTGAAAG AGGCAGAGAT CAGAGAGGAT GAGGAGGAGG TGGAGAAGAA GCAGCAGAAG 
AAGGAGAAGC GTGAGACGGG CAAGGCCATG GTGCCCCACA GCTCGATGTT CATCTTCAGC 
ACCACCAACC CGATCCGCAG GGCCTGCCAC TACATCGTGA ACCTGCGCTA CTTCGAGATG 
TGCATCCTCC TGGTGATCGC AGCCAGCAGC ATCGCCCTGG CGGCCGAGGA CCCCGTCCTG 
ACCAACTCAG AGCGCAACAA AGTGCTGAGG TATTTTGACT ATGTGTTCAC TGGCGTGTTC 
ACCTTTGAGA TGGTTATAAA GATGATAGAC CAGGGCTTGA TTCTGCAGGA CGGCTCCTAC 
TTCCGAGACC TGTGGAACAT CCTGGACTTC GTGGTGGTGG TTGGCGCATT GGTGGCCTTT 
GCTCTGGCGA ATGCTTTGGG AACTAACAAG GGCCGGGATA TTAAGACCAT CAAGTCCCTG 
AGGGTTCTCC GTGTCCTGAG GCCGCTGAAG ACCATCAAGC GCCTGCCCAA GCTCAAGGCT 
GTCTTTGACT GTGTGGTCAC CTCCTTGAAA AACGTCTTCA ACATACTCAT TGTCTACAAG 
CTCTTCATGT TCATCTTTGC TGTCATCGCA GTTCAGCTTT TCAAGGGCAA GTTCTTCTAC 
TGCACGGACA GTTCCAAGGA CACAGAGAAG GAGTGCATAG GCAACTATGT AGATCACGAG 
AAAAACAAGA TGGAGGTGAA AGGCCGAGAG TGGAAGCGCC ATGAATTCCA TTATGACAAC 
ATCATCTGGG CTCTGCTGAC CCTCTTCACT GTCTCCACAG GGGAAGGGTG GCCTCAGGTC 
TTGCAGCACT CGGTCGATGT GACAGAGGAA GACCGAGGCC CAAGCCGCAG CAACCGGATG 
GAGATGTCCA TCTTTTATGT GGTCTACTTT GTGGTCTTCC CCTTCTTCTT TGTCAACATC 
TTTGTGGCTC TCATCATCAT CACCTTCCAG GAGCAAGGGG ACAAGATGAT GGAAGAGTGC 
AGCCTGGAGA AGAATGAGAG GGCATGCATC GACTTCGCCA TCAGCGCCAA ACCGCTCACC 
CGCTACATGC CCCAGAACAG GCACACCTTC CAGTACCGCG TCTGGCACTT CGTGGTGTCT 
CCATCCTTTG AGTACACCAT CATGGCCATG ATCGCCCTGA ACACCGTCGT GCTGATGATG 
AAGTACTACT CTGCTCCCTG TACCTATGAG CTGGCCCTGA AGTACCTAAA TATCGCCTTC 
ACCATGGTGT TTTCCCTGGA ATGTGTCCTG AAGATCATTG CATTTGGCTT CTTGAACTAT 
TTCCGAGACA CCTGGAATAT CTTTGACTTC ATCACTGTGA TTGGCAGTAT CACAGAAATT 
ATTCTGACGG ACAGCAAGCT GGTGAACACT AGTGGCTTCA ACATGAGCTT TCTGAAGCTC 
TTCCGAGCGG CCCGCCTCAT CAAGCTGCTG CGTCAGGGCT ACACCATCCG CATTTTACTG 
TGGACCTTCG TGCAGTCCTT TAAGGCCCTT CCCTATGTCT GCCTTCTGAT CGCCATGCTT 
TTCTTCATCT ATGCCATCAT TGGGATGCAG GTCTTTGGAA ACATAAAGTT AGATGAGGAG 
AGTCACATCA ACCGGCACAA CAACTTCCGG AGTTTCTTTG GGTCCCTCAT GCTGCTCTTC 
AGGAGTGCCA CGGGTGAGGC CTGGCAGGAG ATAATGCTGT CGTGTCTCGG GGAGAAGGGC 
TGTGAGCCCG ACACCACTGC GCCGTCGGGG CAGAGCGAGA GCGAGCGCTG TGGCACCGAC 
CTGGCCTACG TGTACTTCGT CTCCTTCATC TTCTTCTGCT CCTTCCTGAT GCTCAACCTG 
TTCGTGGCTG TCATCATGGA CAACTTTGAG TACCTGACTC GGGACTCGTC CATCCTGGGG 
CCTCACCATC TGGACGAGTT TGTCCGAGTC TGGGCAGAGT ATGACAGAGC AGCATGTGGC 
CGCATCCATT ACACTGAGAT GTATGAAATG CTGACTCTCA TGTCACCGCC GCTAGGCCTC 
GGCAAGAGAT GTCCCTCCAA GGTGGCATAT AAGAGGTTGG TCTTGATGAA CATGCCAGTG 
GCCGAGGACA TGACCGTCCA CTTCACTTCC ACGCTGATGG CTCTAATCCG GACAGCTCTG 
GACATTAAAA TCGCCAAAGG CGGTGCAGAC AGGCAGCAGC TAGACTCAGA GCTCCAGAAG 
GAGACCCTGG CCATCTGGCC TCACCTGTCC CAGAAGATGC TGGATCTGCT CGTGCCCATG 
CCCAAAGCCT CTGACCTGAC TGTGGGCAAA ATCTATGCAG CAATGATGAT CATGGACTAC 
TACAAACAGA GCAAGGTGAA GAAGCAGAGG CAGCAGCTGG AGGAACAGAA AAACGCACCC 
ATGTTCCAGC GCATGGAGCC CTCGTCTCTG CCTCAGGAGA TCATCGCTAA TGCCAAAGCC 
CTGCCTTATC TCCAGCAGGA CCCCGTTCCA GGCCTGAGTG GCCGGAGTGC ATACCCTTCG 
ATGAGTCCAC TCTCCCCGCA GGAAATATTC CAGTTGGCCT GTATGGACCC AGCTGATGAT 
GGACAGTTCC AGGAACGACA ATCTCTGGTG GTGACAGACC CCAGCTCCAT GAGACGTTCA 
TTTTCCACCA TTCGCGATAA GCGTTCAAAT TCCTCGTGGC TGGAGGAATT CTCCATGGAG 
CGAAGCAGCG AAAACACCTA CAAGTCCCGG CGCCGGAGTT ACCACTCCTC CCTGAGGCTG 
TCAGCCCACC GTCTGAACTC GGACTCAGGC CACAAGTCCG ACACCCACCG CTCAGGGGGC 
AGAGAGCGGG GACGATCGAA AGAGCGAAAG CATCTTCTCT CTCCTGACGT CTCCCGCTGC 
AACTCCGAGG AGCGAGGGAC CCAGGCCGAC TGGGAGTCCC CAGAGCGCCA TCAGTCCCGG 
TCTCCCAGTG AAGGCAGGTC ACAGACCCCC AACAGACAGG GCACGGGGTC CCTGAGTGAG 
AGCTCCATCC CCTCTGTCTC CGACTCCAGC ACCCCACGAC GAAGTCGGCG GCAGCTCCCC 
CCAGTCCCTC CCAAGCCTCG GCCCCTCCTT TCCTACAGCT CTCTGATCAG ACACGCTGGG 
AGCATCTCCC CACCAGCTGA TGGGAGCGAG GGTGGCTCCC CACTGACCTC CCAAGCTCTG 
GAGAGCAACG ACGCCGGCCT GCCCGAGTCT TCCAACCCCC GGCAGGGCCA CCCCTCCCCA 
CAGCGCTACA TCTCCGAGCC CTACCTGGCC CTGCACGAAG ACTCCCACGC CTCGGACTGC 
GGCGAGGAGG AGACGCTCAC CTTTGAAGCA GCCGTGGCCA CCAGCCTGGG CCGGTCCAAC 
ACCATCGGCT CGGCCCCGCC CCTGCGGCAC AGCTGGCAGA TGCCCAATGG GCACTATCGG 
CGGCGGAGGC GCGGGGGGCC TGGACCGGGC ACGACGTGCG GAGCCGTCAG CGACCTCCTG 
AGTGACACGG AAGAAGACGA CAAATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330735.1
CDS	1..6750
Translation	MARFGEAVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECRDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNAGTSALEVLRRATIKRSRTEAMARDSSDEHCVDISSVGTPLARASIKSAKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSLVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLSAHPSLYRRPRAIEGLALGLGLEKCEEERVSRGGSLKGDGGDLASALDSQRSPLALGKREPPWLARPCHGNCDPTQPEAGSGETVVTFGDRARHRQSQRRSRHRRVRTEGREPSSTSRSRSASQERSLDEAVPSEGERDREPRAGHGGKEPMIQEEERERCQDLRRTNSLMVPRGSGLAGALDEADTPLVPPQPELEVGKDAALTEQEPEGSSEQALLGDVKLDIGRVSQSEPDLSCVTANTDKATTESTSVTVTIPDVGPLVDSTVVHISNKTDGEASPLKEAEIREDEEEVEKKQQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKIIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQSESERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVPGLSGRSAYPSMSPLSPQEIFQLACMDPADDGQFQERQSLVVTDPSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERHQSRSPSEGRSQTPNRQGTGSLSESSIPSVSDSSTPRRSRRQLPPVPPKPRPLLSYSSLIRHAGSISPPADGSEGGSPLTSQALESNDAGLPESSNPRQGHPSPQRYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGTTCGAVSDLLSDTEEDDKC

Target ORF information:

RefSeq Version	XM_015475249.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015475249.1

ORF Insert Sequence:

ATGGCTCGCT TCGGGGAGGC GGTGGTCGGC AGGCCGGGGT CGGGCGATGG AGACTCGGAC 
CAGAGCAGGA ACCGGCAAGG AACCCCCGTG CCGGCCTCCG GGCCGGCGGC CGCCTACAAG 
CAGTCGAAAG CGCAGAGGGC GCGGACTATG GCTTTGTACA ACCCTATTCC CGTCCGGCAG 
AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA 
TACGCCAAGA AGCTCATCGA TTGGCCGCCG TTTGAGTACA TGATCTTGGC CACCATCATT 
GCCAATTGCA TCGTCTTGGC CCTGGAGCAA CATCTTCCTG AGGATGACAA GACCCCAATG 
TCCCGCAGAC TGGAGAAGAC AGAACCCTAT TTCATTGGGA TCTTTTGCTT TGAAGCTGGG 
ATCAAAATCG TGGCCCTGGG GTTCATCTTC CATAAGGGAT CATACCTCCG CAATGGTTGG 
AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CTACTGCGGG AACCCACTTC 
AACACCCATG TGGACCTGAG GACCCTCCGG GCCGTGCGTG TCCTGAGGCC CCTGAAGCTC 
GTGTCGGGGA TACCTAGCCT ACAGATTGTG TTGAAATCCA TCATGAAGGC CATGGTGCCT 
CTCCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATCCTGA TGTTTGCTAT CATCGGCTTG 
GAGTTCTACA GTGGAAAGTT GCACCGAGCG TGCTTCATGA ACAATTCAGG TATTCTAGAA 
GGATTTGACC CCCCTCACCC ATGTGGCGTG CAGGGCTGCC CAGCTGGTTA TGAATGCCGG 
GACTGGATCG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCCGTGCTG 
ACCGTCTTCC AGTGTATCAC CATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT 
GCCTTAGGAG CCACCTGGAA TTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC 
TTTGTTCTCA ACCTTGTCCT GGGAGTGCTT TCTGGGGAAT TTGCCAAAGA GAGAGAGAGA 
GTGGAGAACC GGAGAGCCTT CATGAAACTG CGGCGCCAGC AGCAGATCGA GCGTGAGCTT 
AACGGGTACC GCGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTTGCTGA AGAAAACAAA 
AATGCTGGAA CGTCAGCCTT AGAAGTGCTT CGGAGGGCAA CCATCAAAAG GAGCCGGACC 
GAGGCCATGG CACGGGACTC CAGCGATGAG CACTGCGTGG ACATCTCCTC TGTGGGCACA 
CCTCTCGCCC GAGCCAGCAT CAAGAGCGCC AAGGTGGACG GGGCCTCCTA TTTCCGGCAC 
AAGGAGCGAC TTTTGCGGAT CTCTATCCGC CACATGGTTA AGTCCCAGGT GTTCTACTGG 
ATCGTGCTGA GCCTGGTGGC TCTCAACACC GCCTGTGTGG CCATCGTCCA CCACAACCAG 
CCCCAGTGGC TCACCCACCT CCTCTACTAT GCAGAGTTTC TGTTTCTGGG GCTCTTCCTT 
CTAGAGATGT CCCTGAAGAT GTACGGCATG GGGCCTCGCC TTTATTTTCA CTCTTCCTTC 
AACTGCTTTG ACTTTGGGGT CACAGTGGGC AGCATCTTTG AAGTAGTCTG GGCCATCTTC 
AGACCTGGTA CATCTTTCGG AATCAGTGTC CTGCGAGCTC TCCGGCTTCT AAGAATATTT 
AAAATAACCA AGTATTGGGC ATCCCTCCGG AATTTGGTGG TCTCCTTGAT GAGTTCCATG 
AAGTCTATCA TCAGTTTACT CTTCCTCCTT TTCCTCTTCA TTGTTGTCTT TGCTCTGCTA 
GGAATGCAGC TGTTTGGGGG CAGGTTCAAC TTTAATGATG GGACTCCTTC CGCAAACTTT 
GACACCTTCC CTGCAGCCAT CATGACTGTA TTCCAGATCC TGACGGGTGA GGACTGGAAT 
GAGGTGATGT ACAACGGGAT CCGCTCCCAG GGTGGGGTCA GCTCGGGCAT GTGGTCTGCG 
ATCTATTTCA TTGTGCTTAC CTTGTTTGGA AACTACACAC TGCTGAATGT GTTCTTGGCC 
ATCGCTGTGG ACAATCTGGC CAACGCCCAG GAGCTGACCA AGGATGAACA AGAAGAAGAG 
GAGGCCTTCA ACCAGAAGCA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA 
CCCAACATGC CTTCCATCGA GAGGGAGCGG AGGCGCCGGC ATCACATGTC CGTGTGGGAG 
CAGCGAACCA GCCAGTTGAG GAGGCACATG CAGATGTCCA GCCAGGAGGC CCTCAACAAG 
GAGGAGGCCC CGCCCATGAA CCCCCTCAAC CCCCTCAACC CGCTGAGCCC CCTCAACCCG 
CTCAGCGCGC ACCCCAGCCT CTACCGGAGA CCCCGGGCCA TCGAGGGGCT AGCCCTGGGC 
CTGGGGCTGG AGAAGTGTGA GGAAGAGCGC GTCAGCCGTG GGGGGTCCCT CAAAGGGGAT 
GGAGGGGACT TGGCGAGCGC CCTGGACAGC CAGAGGAGCC CCCTGGCCCT GGGCAAACGG 
GAGCCACCCT GGCTGGCCCG GCCCTGTCAC GGGAACTGTG ACCCGACCCA GCCGGAGGCG 
GGCAGTGGGG AGACGGTGGT GACCTTCGGG GACCGGGCCC GGCACAGGCA GAGCCAGCGG 
CGCAGCCGGC ACCGCCGCGT CAGGACCGAG GGCAGGGAGC CCTCCTCCAC CTCCAGAAGC 
AGGTCTGCCA GCCAGGAGCG GAGTCTGGAC GAAGCTGTGC CCAGCGAGGG GGAGAGGGAC 
CGTGAGCCCA GGGCTGGCCA TGGGGGCAAA GAACCCATGA TCCAGGAGGA AGAGCGAGAG 
CGATGCCAGG ACTTACGGAG GACCAACAGT CTGATGGTGC CCAGAGGCTC CGGGCTGGCA 
GGAGCCCTGG ATGAGGCCGA CACACCCCTT GTCCCACCCC AGCCGGAGCT GGAAGTGGGG 
AAGGACGCAG CCCTGACGGA GCAGGAGCCG GAGGGCAGCA GTGAGCAGGC TCTGCTGGGG 
GACGTGAAGC TGGACATAGG CCGGGTCAGC CAGAGCGAGC CCGACCTCTC CTGCGTCACG 
GCCAACACGG ACAAGGCCAC CACCGAGAGC ACCAGCGTCA CTGTCACCAT CCCCGACGTG 
GGCCCCTTGG TGGACTCAAC CGTGGTGCAC ATCAGCAACA AGACTGATGG AGAAGCCAGT 
CCCTTGAAAG AGGCAGAGAT CAGAGAGGAT GAGGAGGAGG TGGAGAAGAA GCAGCAGAAG 
AAGGAGAAGC GTGAGACGGG CAAGGCCATG GTGCCCCACA GCTCGATGTT CATCTTCAGC 
ACCACCAACC CGATCCGCAG GGCCTGCCAC TACATCGTGA ACCTGCGCTA CTTCGAGATG 
TGCATCCTCC TGGTGATCGC AGCCAGCAGC ATCGCCCTGG CGGCCGAGGA CCCCGTCCTG 
ACCAACTCAG AGCGCAACAA AGTGCTGAGG TATTTTGACT ATGTGTTCAC TGGCGTGTTC 
ACCTTTGAGA TGGTTATAAA GATGATAGAC CAGGGCTTGA TTCTGCAGGA CGGCTCCTAC 
TTCCGAGACC TGTGGAACAT CCTGGACTTC GTGGTGGTGG TTGGCGCATT GGTGGCCTTT 
GCTCTGGCGA ATGCTTTGGG AACTAACAAG GGCCGGGATA TTAAGACCAT CAAGTCCCTG 
AGGGTTCTCC GTGTCCTGAG GCCGCTGAAG ACCATCAAGC GCCTGCCCAA GCTCAAGGCT 
GTCTTTGACT GTGTGGTCAC CTCCTTGAAA AACGTCTTCA ACATACTCAT TGTCTACAAG 
CTCTTCATGT TCATCTTTGC TGTCATCGCA GTTCAGCTTT TCAAGGGCAA GTTCTTCTAC 
TGCACGGACA GTTCCAAGGA CACAGAGAAG GAGTGCATAG GCAACTATGT AGATCACGAG 
AAAAACAAGA TGGAGGTGAA AGGCCGAGAG TGGAAGCGCC ATGAATTCCA TTATGACAAC 
ATCATCTGGG CTCTGCTGAC CCTCTTCACT GTCTCCACAG GGGAAGGGTG GCCTCAGGTC 
TTGCAGCACT CGGTCGATGT GACAGAGGAA GACCGAGGCC CAAGCCGCAG CAACCGGATG 
GAGATGTCCA TCTTTTATGT GGTCTACTTT GTGGTCTTCC CCTTCTTCTT TGTCAACATC 
TTTGTGGCTC TCATCATCAT CACCTTCCAG GAGCAAGGGG ACAAGATGAT GGAAGAGTGC 
AGCCTGGAGA AGAATGAGAG GGCATGCATC GACTTCGCCA TCAGCGCCAA ACCGCTCACC 
CGCTACATGC CCCAGAACAG GCACACCTTC CAGTACCGCG TCTGGCACTT CGTGGTGTCT 
CCATCCTTTG AGTACACCAT CATGGCCATG ATCGCCCTGA ACACCGTCGT GCTGATGATG 
AAGTACTACT CTGCTCCCTG TACCTATGAG CTGGCCCTGA AGTACCTAAA TATCGCCTTC 
ACCATGGTGT TTTCCCTGGA ATGTGTCCTG AAGATCATTG CATTTGGCTT CTTGAACTAT 
TTCCGAGACA CCTGGAATAT CTTTGACTTC ATCACTGTGA TTGGCAGTAT CACAGAAATT 
ATTCTGACGG ACAGCAAGCT GGTGAACACT AGTGGCTTCA ACATGAGCTT TCTGAAGCTC 
TTCCGAGCGG CCCGCCTCAT CAAGCTGCTG CGTCAGGGCT ACACCATCCG CATTTTACTG 
TGGACCTTCG TGCAGTCCTT TAAGGCCCTT CCCTATGTCT GCCTTCTGAT CGCCATGCTT 
TTCTTCATCT ATGCCATCAT TGGGATGCAG GTCTTTGGAA ACATAAAGTT AGATGAGGAG 
AGTCACATCA ACCGGCACAA CAACTTCCGG AGTTTCTTTG GGTCCCTCAT GCTGCTCTTC 
AGGAGTGCCA CGGGTGAGGC CTGGCAGGAG ATAATGCTGT CGTGTCTCGG GGAGAAGGGC 
TGTGAGCCCG ACACCACTGC GCCGTCGGGG CAGAGCGAGA GCGAGCGCTG TGGCACCGAC 
CTGGCCTACG TGTACTTCGT CTCCTTCATC TTCTTCTGCT CCTTCCTGAT GCTCAACCTG 
TTCGTGGCTG TCATCATGGA CAACTTTGAG TACCTGACTC GGGACTCGTC CATCCTGGGG 
CCTCACCATC TGGACGAGTT TGTCCGAGTC TGGGCAGAGT ATGACAGAGC AGCATGTGGC 
CGCATCCATT ACACTGAGAT GTATGAAATG CTGACTCTCA TGTCACCGCC GCTAGGCCTC 
GGCAAGAGAT GTCCCTCCAA GGTGGCATAT AAGAGGTTGG TCTTGATGAA CATGCCAGTG 
GCCGAGGACA TGACCGTCCA CTTCACTTCC ACGCTGATGG CTCTAATCCG GACAGCTCTG 
GACATTAAAA TCGCCAAAGG CGGTGCAGAC AGGCAGCAGC TAGACTCAGA GCTCCAGAAG 
GAGACCCTGG CCATCTGGCC TCACCTGTCC CAGAAGATGC TGGATCTGCT CGTGCCCATG 
CCCAAAGCCT CTGACCTGAC TGTGGGCAAA ATCTATGCAG CAATGATGAT CATGGACTAC 
TACAAACAGA GCAAGGTGAA GAAGCAGAGG CAGCAGCTGG AGGAACAGAA AAACGCACCC 
ATGTTCCAGC GCATGGAGCC CTCGTCTCTG CCTCAGGAGA TCATCGCTAA TGCCAAAGCC 
CTGCCTTATC TCCAGCAGGA CCCCGTTCCA GGCCTGAGTG GCCGGAGTGC ATACCCTTCG 
ATGAGTCCAC TCTCCCCGCA GGAAATATTC CAGTTGGCCT GTATGGACCC AGCTGATGAT 
GGACAGTTCC AGGAACGACA ATCTCTGGTG GTGACAGACC CCAGCTCCAT GAGACGTTCA 
TTTTCCACCA TTCGCGATAA GCGTTCAAAT TCCTCGTGGC TGGAGGAATT CTCCATGGAG 
CGAAGCAGCG AAAACACCTA CAAGTCCCGG CGCCGGAGTT ACCACTCCTC CCTGAGGCTG 
TCAGCCCACC GTCTGAACTC GGACTCAGGC CACAAGTCCG ACACCCACCG CTCAGGGGGC 
AGAGAGCGGG GACGATCGAA AGAGCGAAAG CATCTTCTCT CTCCTGACGT CTCCCGCTGC 
AACTCCGAGG AGCGAGGGAC CCAGGCCGAC TGGGAGTCCC CAGAGCGCCA TCAGTCCCGG 
TCTCCCAGTG AAGGCAGGTC ACAGACCCCC AACAGACAGG GCACGGGGTC CCTGAGTGAG 
AGCTCCATCC CCTCTGTCTC CGACTCCAGC ACCCCACGAC GAAGTCGGCG GCAGCTCCCC 
CCAGTCCCTC CCAAGCCTCG GCCCCTCCTT TCCTACAGCT CTCTGATCAG ACACGCTGGG 
AGCATCTCCC CACCAGCTGA TGGGAGCGAG GGTGGCTCCC CACTGACCTC CCAAGCTCTG 
GAGAGCAACG ACGCCGGCCT GCCCGAGTCT TCCAACCCCC GGCAGGGCCA CCCCTCCCCA 
CAGCGCTACA TCTCCGAGCC CTACCTGGCC CTGCACGAAG ACTCCCACGC CTCGGACTGC 
GGCGAGGAGG AGACGCTCAC CTTTGAAGCA GCCGTGGCCA CCAGCCTGGG CCGGTCCAAC 
ACCATCGGCT CGGCCCCGCC CCTGCGGCAC AGCTGGCAGA TGCCCAATGG GCACTATCGG 
CGGCGGAGGC GCGGGGGGCC TGGACCGGGC ACGACGTGCG GAGCCGTCAG CGACCTCCTG 
AGTGACACGG AAGAAGACGA CAAATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1E ( NM_001191123.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001178052.1 Bos taurus calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), mRNA.

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CloneID	OBa01606
Clone ID Related Accession (Same CDS sequence)	NM_001191123.1
Accession Version	NM_001191123.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6807bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2011-10-22
Organism	Bos taurus(Cattle)
Product	voltage-dependent R-type calcium channel subunit alpha-1E
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03009541.1, AAFC03009542.1, AAFC03028899.1, AAFC03028900.1 and AAFC03070038.1. On Jul 17, 2010 this sequence version replaced XM_001249409.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

ATGGCTCGCT TCGGGGAGGC GGTGGTCGGC AGGCCGGGGT CGGGCGATGG AGACTCGGAC 
CAGAGCAGGA ACCGGCAAGG AACCCCCGTG CCGGCCTCCG GGCCGGCGGC CGCCTACAAG 
CAGTCGAAAG CGCAGAGGGC GCGGACTATG GCTTTGTACA ACCCTATTCC CGTCCGGCAG 
AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA 
TACGCCAAGA AGCTCATCGA TTGGCCGCCG TTTGAGTACA TGATCTTGGC CACCATCATT 
GCCAATTGCA TCGTCTTGGC CCTGGAGCAA CATCTTCCTG AGGATGACAA GACCCCAATG 
TCCCGCAGAC TGGAGAAGAC AGAACCCTAT TTCATTGGGA TCTTTTGCTT TGAAGCTGGG 
ATCAAAATCG TGGCCCTGGG GTTCATCTTC CATAAGGGAT CATACCTCCG CAATGGTTGG 
AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CTACTGCGGG AACCCACTTC 
AACACCCATG TGGACCTGAG GACCCTCCGG GCCGTGCGTG TCCTGAGGCC CCTGAAGCTC 
GTGTCGGGGA TACCTAGCCT ACAGATTGTG TTGAAATCCA TCATGAAGGC CATGGTGCCT 
CTCCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATCCTGA TGTTTGCTAT CATCGGCTTG 
GAGTTCTACA GTGGAAAGTT GCACCGAGCG TGCTTCATGA ACAATTCAGG TATTCTAGAA 
GGATTTGACC CCCCTCACCC ATGTGGCGTG CAGGGCTGCC CAGCTGGTTA TGAATGCCGG 
GACTGGATCG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCCGTGCTG 
ACCGTCTTCC AGTGTATCAC CATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT 
GCCTTAGGAG CCACCTGGAA TTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC 
TTTGTTCTCA ACCTTGTCCT GGGAGTGCTT TCTGGGGAAT TTGCCAAAGA GAGAGAGAGA 
GTGGAGAACC GGAGAGCCTT CATGAAACTG CGGCGCCAGC AGCAGATCGA GCGTGAGCTT 
AACGGGTACC GCGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTTGCTGA AGAAAACAAA 
AATGCTGGAA CGTCAGCCTT AGAAGTGCTT CGGAGGGCAA CCATCAAAAG GAGCCGGACC 
GAGGCCATGG CACGGGACTC CAGCGATGAG CACTGCGTGG ACATCTCCTC TGTGGGCACA 
CCTCTCGCCC GAGCCAGCAT CAAGAGCGCC AAGGTGGACG GGGCCTCCTA TTTCCGGCAC 
AAGGAGCGAC TTTTGCGGAT CTCTATCCGC CACATGGTTA AGTCCCAGGT GTTCTACTGG 
ATCGTGCTGA GCCTGGTGGC TCTCAACACC GCCTGTGTGG CCATCGTCCA CCACAACCAG 
CCCCAGTGGC TCACCCACCT CCTCTACTAT GCAGAGTTTC TGTTTCTGGG GCTCTTCCTT 
CTAGAGATGT CCCTGAAGAT GTACGGCATG GGGCCTCGCC TTTATTTTCA CTCTTCCTTC 
AACTGCTTTG ACTTTGGGGT CACAGTGGGC AGCATCTTTG AAGTAGTCTG GGCCATCTTC 
AGACCTGGTA CATCTTTCGG AATCAGTGTC CTGCGAGCTC TCCGGCTTCT AAGAATATTT 
AAAATAACCA AGTATTGGGC ATCCCTCCGG AATTTGGTGG TCTCCTTGAT GAGTTCCATG 
AAGTCTATCA TCAGTTTACT CTTCCTCCTT TTCCTCTTCA TTGTTGTCTT TGCTCTGCTA 
GGAATGCAGC TGTTTGGGGG CAGGTTCAAC TTTAATGATG GGACTCCTTC CGCAAACTTT 
GACACCTTCC CTGCAGCCAT CATGACTGTA TTCCAGATCC TGACGGGTGA GGACTGGAAT 
GAGGTGATGT ACAACGGGAT CCGCTCCCAG GGTGGGGTCA GCTCGGGCAT GTGGTCTGCG 
ATCTATTTCA TTGTGCTTAC CTTGTTTGGA AACTACACAC TGCTGAATGT GTTCTTGGCC 
ATCGCTGTGG ACAATCTGGC CAACGCCCAG GAGCTGACCA AGGATGAACA AGAAGAAGAG 
GAGGCCTTCA ACCAGAAGCA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA 
CCCAACATGC CTTCCATCGA AAGAGACAGA AGGAGAAGAC ACCACATGTC GATGTGGGAG 
CCACGCAGCA GCCACCTGAG GGAGCGGAGG CGCCGGCATC ACATGTCCGT GTGGGAGCAG 
CGAACCAGCC AGTTGAGGAG GCACATGCAG ATGTCCAGCC AGGAGGCCCT CAACAAGGAG 
GAGGCCCCGC CCATGAACCC CCTCAACCCC CTCAACCCGC TGAGCCCCCT CAACCCGCTC 
AGCGCGCACC CCAGCCTCTA CCGGAGACCC CGGGCCATCG AGGGGCTAGC CCTGGGCCTG 
GGGCTGGAGA AGTGTGAGGA AGAGCGCGTC AGCCGTGGGG GGTCCCTCAA AGGGGATGGA 
GGGGACTTGG CGAGCGCCCT GGACAGCCAG AGGAGCCCCC TGGCCCTGGG CAAACGGGAG 
CCACCCTGGC TGGCCCGGCC CTGTCACGGG AACTGTGACC CGACCCAGCC GGAGGCGGGC 
AGTGGGGAGA CGGTGGTGAC CTTCGGGGAC CGGGCCCGGC ACAGGCAGAG CCAGCGGCGC 
AGCCGGCACC GCCGCGTCAG GACCGAGGGC AGGGAGCCCT CCTCCACCTC CAGAAGCAGG 
TCTGCCAGCC AGGAGCGGAG TCTGGACGAA GCTGTGCCCA GCGAGGGGGA GAGGGACCGT 
GAGCCCAGGG CTGGCCATGG GGGCAAAGAA CCCATGATCC AGGAGGAAGA GCGAGAGCGA 
TGCCAGGACT TACGGAGGAC CAACAGTCTG ATGGTGCCCA GAGGCTCCGG GCTGGCAGGA 
GCCCTGGATG AGGCCGACAC ACCCCTTGTC CCACCCCAGC CGGAGCTGGA AGTGGGGAAG 
GACGCAGCCC TGACGGAGCA GGAGCCGGAG GGCAGCAGTG AGCAGGCTCT GCTGGGGGAC 
GTGAAGCTGG ACATAGGCCG GGTCAGCCAG AGCGAGCCCG ACCTCTCCTG CGTCACGGCC 
AACACGGACA AGGCCACCAC CGAGAGCACC AGCGTCACTG TCACCATCCC CGACGTGGGC 
CCCTTGGTGG ACTCAACCGT GGTGCACATC AGCAACAAGA CTGATGGAGA AGCCAGTCCC 
TTGAAAGAGG CAGAGATCAG AGAGGATGAG GAGGAGGTGG AGAAGAAGCA GCAGAAGAAG 
GAGAAGCGTG AGACGGGCAA GGCCATGGTG CCCCACAGCT CGATGTTCAT CTTCAGCACC 
ACCAACCCGA TCCGCAGGGC CTGCCACTAC ATCGTGAACC TGCGCTACTT CGAGATGTGC 
ATCCTCCTGG TGATCGCAGC CAGCAGCATC GCCCTGGCGG CCGAGGACCC CGTCCTGACC 
AACTCAGAGC GCAACAAAGT GCTGAGGTAT TTTGACTATG TGTTCACTGG CGTGTTCACC 
TTTGAGATGG TTATAAAGAT GATAGACCAG GGCTTGATTC TGCAGGACGG CTCCTACTTC 
CGAGACCTGT GGAACATCCT GGACTTCGTG GTGGTGGTTG GCGCATTGGT GGCCTTTGCT 
CTGGCGAATG CTTTGGGAAC TAACAAGGGC CGGGATATTA AGACCATCAA GTCCCTGAGG 
GTTCTCCGTG TCCTGAGGCC GCTGAAGACC ATCAAGCGCC TGCCCAAGCT CAAGGCTGTC 
TTTGACTGTG TGGTCACCTC CTTGAAAAAC GTCTTCAACA TACTCATTGT CTACAAGCTC 
TTCATGTTCA TCTTTGCTGT CATCGCAGTT CAGCTTTTCA AGGGCAAGTT CTTCTACTGC 
ACGGACAGTT CCAAGGACAC AGAGAAGGAG TGCATAGGCA ACTATGTAGA TCACGAGAAA 
AACAAGATGG AGGTGAAAGG CCGAGAGTGG AAGCGCCATG AATTCCATTA TGACAACATC 
ATCTGGGCTC TGCTGACCCT CTTCACTGTC TCCACAGGGG AAGGGTGGCC TCAGGTCTTG 
CAGCACTCGG TCGATGTGAC AGAGGAAGAC CGAGGCCCAA GCCGCAGCAA CCGGATGGAG 
ATGTCCATCT TTTATGTGGT CTACTTTGTG GTCTTCCCCT TCTTCTTTGT CAACATCTTT 
GTGGCTCTCA TCATCATCAC CTTCCAGGAG CAAGGGGACA AGATGATGGA AGAGTGCAGC 
CTGGAGAAGA ATGAGAGGGC ATGCATCGAC TTCGCCATCA GCGCCAAACC GCTCACCCGC 
TACATGCCCC AGAACAGGCA CACCTTCCAG TACCGCGTCT GGCACTTCGT GGTGTCTCCA 
TCCTTTGAGT ACACCATCAT GGCCATGATC GCCCTGAACA CCGTCGTGCT GATGATGAAG 
TACTACTCTG CTCCCTGTAC CTATGAGCTG GCCCTGAAGT ACCTAAATAT CGCCTTCACC 
ATGGTGTTTT CCCTGGAATG TGTCCTGAAG ATCATTGCAT TTGGCTTCTT GAACTATTTC 
CGAGACACCT GGAATATCTT TGACTTCATC ACTGTGATTG GCAGTATCAC AGAAATTATT 
CTGACGGACA GCAAGCTGGT GAACACTAGT GGCTTCAACA TGAGCTTTCT GAAGCTCTTC 
CGAGCGGCCC GCCTCATCAA GCTGCTGCGT CAGGGCTACA CCATCCGCAT TTTACTGTGG 
ACCTTCGTGC AGTCCTTTAA GGCCCTTCCC TATGTCTGCC TTCTGATCGC CATGCTTTTC 
TTCATCTATG CCATCATTGG GATGCAGGTC TTTGGAAACA TAAAGTTAGA TGAGGAGAGT 
CACATCAACC GGCACAACAA CTTCCGGAGT TTCTTTGGGT CCCTCATGCT GCTCTTCAGG 
AGTGCCACGG GTGAGGCCTG GCAGGAGATA ATGCTGTCGT GTCTCGGGGA GAAGGGCTGT 
GAGCCCGACA CCACTGCGCC ATCGGGGCAG AGCGAGAGCG AGCGCTGTGG CACCGACCTG 
GCCTACGTGT ACTTCGTCTC CTTCATCTTC TTCTGCTCCT TCCTGATGCT CAACCTGTTC 
GTGGCCGTCA TCATGGACAA CTTTGAGTAC CTGACTCGGG ACTCGTCCAT CCTGGGGCCT 
CACCATCTGG ACGAGTTTGT CCGAGTCTGG GCAGAGTATG ACAGAGCAGC ATGTGGCCGC 
ATCCATTACA CTGAGATGTA TGAAATGCTG ACTCTCATGT CACCGCCGCT AGGCCTCGGC 
AAGAGATGTC CCTCCAAGGT GGCATATAAG AGGTTGGTCT TGATGAACAT GCCAGTGGCC 
GAGGACATGA CCGTCCACTT CACTTCCACG CTGATGGCTC TAATCCGGAC AGCTCTGGAC 
ATTAAAATCG CCAAAGGCGG TGCAGACAGG CAGCAGCTAG ACTCAGAGCT CCAGAAGGAG 
ACCCTGGCCA TCTGGCCTCA CCTGTCCCAG AAGATGCTGG ATCTGCTCGT GCCCATGCCC 
AAAGCCTCTG ACCTGACTGT GGGCAAAATC TATGCAGCAA TGATGATCAT GGACTACTAC 
AAACAGAGCA AGGTGAAGAA GCAGAGGCAG CAGCTGGAGG AACAGAAAAA CGCACCCATG 
TTCCAGCGCA TGGAGCCCTC GTCTCTGCCT CAGGAGATCA TCGCTAATGC CAAAGCCCTG 
CCTTATCTCC AGCAGGACCC CGTTCCAGGC CTGAGTGGCC GGAGTGCATA CCCTTCGATG 
AGTCCACTCT CCCCGCAGGA AATATTCCAG TTGGCCTGTA TGGACCCAGC TGATGATGGA 
CAGTTCCAGG AACGACAATC TCTGGTGGTG ACAGACCCCA GCTCCATGAG ACGTTCATTT 
TCCACCATTC GCGATAAGCG TTCAAATTCC TCGTGGCTGG AGGAATTCTC CATGGAGCGA 
AGCAGCGAAA ACACCTACAA GTCCCGGCGC CGGAGTTACC ACTCCTCCCT GAGGCTGTCA 
GCCCACCGTC TGAACTCGGA CTCAGGCCAC AAGTCCGACA CCCACCGCTC AGGGGGCAGA 
GAGCGGGGAC GATCGAAAGA GCGAAAGCAT CTTCTCTCTC CTGACGTCTC CCGCTGCAAC 
TCCGAGGAGC GAGGGACCCA GGCCGACTGG GAGTCCCCAG AGCGCCATCA GTCCCGGTCT 
CCCAGTGAAG GCAGGTCACA GACCCCCAAC AGACAGGGCA CGGGGTCCCT GAGTGAGAGC 
TCCATCCCCT CTGTCTCCGA CTCCAGCACC CCACGACGAA GTCGGCGGCA GCTCCCCCCA 
GTCCCTCCCA AGCCTCGGCC CCTCCTTTCC TACAGCTCTC TGATCAGACA CGCTGGGAGC 
ATCTCCCCAC CAGCTGATGG GAGCGAGGGT GGCTCCCCAC TGACCTCCCA AGCTCTGGAG 
AGCAACGACG CCGGCCTGCC CGAGTCTTCC AACCCCCGGC AGGGCCACCC CTCCCCACAG 
CGCTACATCT CCGAGCCCTA CCTGGCCCTG CACGAAGACT CCCACGCCTC GGACTGCGGC 
GAGGAGGAGA CGCTCACCTT TGAAGCAGCC GTGGCCACCA GCCTGGGCCG GTCCAACACC 
ATCGGCTCGG CCCCGCCCCT GCGGCACAGC TGGCAGATGC CCAATGGGCA CTATCGGCGG 
CGGAGGCGCG GGGGGCCTGG ACCGGGCACG ACGTGCGGAG CCGTCAGCGA CCTCCTGAGT 
GACACGGAAG AAGACGACAA ATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001178052.1
CDS	1..6807
Translation	MARFGEAVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECRDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNAGTSALEVLRRATIKRSRTEAMARDSSDEHCVDISSVGTPLARASIKSAKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSLVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLSAHPSLYRRPRAIEGLALGLGLEKCEEERVSRGGSLKGDGGDLASALDSQRSPLALGKREPPWLARPCHGNCDPTQPEAGSGETVVTFGDRARHRQSQRRSRHRRVRTEGREPSSTSRSRSASQERSLDEAVPSEGERDREPRAGHGGKEPMIQEEERERCQDLRRTNSLMVPRGSGLAGALDEADTPLVPPQPELEVGKDAALTEQEPEGSSEQALLGDVKLDIGRVSQSEPDLSCVTANTDKATTESTSVTVTIPDVGPLVDSTVVHISNKTDGEASPLKEAEIREDEEEVEKKQQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKIIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQSESERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVPGLSGRSAYPSMSPLSPQEIFQLACMDPADDGQFQERQSLVVTDPSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERHQSRSPSEGRSQTPNRQGTGSLSESSIPSVSDSSTPRRSRRQLPPVPPKPRPLLSYSSLIRHAGSISPPADGSEGGSPLTSQALESNDAGLPESSNPRQGHPSPQRYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGTTCGAVSDLLSDTEEDDKC

Target ORF information:

RefSeq Version	NM_001191123.1
Organism	Bos taurus(Cattle)
Definition	Bos taurus calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001191123.1

ORF Insert Sequence:

ATGGCTCGCT TCGGGGAGGC GGTGGTCGGC AGGCCGGGGT CGGGCGATGG AGACTCGGAC 
CAGAGCAGGA ACCGGCAAGG AACCCCCGTG CCGGCCTCCG GGCCGGCGGC CGCCTACAAG 
CAGTCGAAAG CGCAGAGGGC GCGGACTATG GCTTTGTACA ACCCTATTCC CGTCCGGCAG 
AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA 
TACGCCAAGA AGCTCATCGA TTGGCCGCCG TTTGAGTACA TGATCTTGGC CACCATCATT 
GCCAATTGCA TCGTCTTGGC CCTGGAGCAA CATCTTCCTG AGGATGACAA GACCCCAATG 
TCCCGCAGAC TGGAGAAGAC AGAACCCTAT TTCATTGGGA TCTTTTGCTT TGAAGCTGGG 
ATCAAAATCG TGGCCCTGGG GTTCATCTTC CATAAGGGAT CATACCTCCG CAATGGTTGG 
AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CTACTGCGGG AACCCACTTC 
AACACCCATG TGGACCTGAG GACCCTCCGG GCCGTGCGTG TCCTGAGGCC CCTGAAGCTC 
GTGTCGGGGA TACCTAGCCT ACAGATTGTG TTGAAATCCA TCATGAAGGC CATGGTGCCT 
CTCCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATCCTGA TGTTTGCTAT CATCGGCTTG 
GAGTTCTACA GTGGAAAGTT GCACCGAGCG TGCTTCATGA ACAATTCAGG TATTCTAGAA 
GGATTTGACC CCCCTCACCC ATGTGGCGTG CAGGGCTGCC CAGCTGGTTA TGAATGCCGG 
GACTGGATCG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCCGTGCTG 
ACCGTCTTCC AGTGTATCAC CATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT 
GCCTTAGGAG CCACCTGGAA TTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC 
TTTGTTCTCA ACCTTGTCCT GGGAGTGCTT TCTGGGGAAT TTGCCAAAGA GAGAGAGAGA 
GTGGAGAACC GGAGAGCCTT CATGAAACTG CGGCGCCAGC AGCAGATCGA GCGTGAGCTT 
AACGGGTACC GCGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTTGCTGA AGAAAACAAA 
AATGCTGGAA CGTCAGCCTT AGAAGTGCTT CGGAGGGCAA CCATCAAAAG GAGCCGGACC 
GAGGCCATGG CACGGGACTC CAGCGATGAG CACTGCGTGG ACATCTCCTC TGTGGGCACA 
CCTCTCGCCC GAGCCAGCAT CAAGAGCGCC AAGGTGGACG GGGCCTCCTA TTTCCGGCAC 
AAGGAGCGAC TTTTGCGGAT CTCTATCCGC CACATGGTTA AGTCCCAGGT GTTCTACTGG 
ATCGTGCTGA GCCTGGTGGC TCTCAACACC GCCTGTGTGG CCATCGTCCA CCACAACCAG 
CCCCAGTGGC TCACCCACCT CCTCTACTAT GCAGAGTTTC TGTTTCTGGG GCTCTTCCTT 
CTAGAGATGT CCCTGAAGAT GTACGGCATG GGGCCTCGCC TTTATTTTCA CTCTTCCTTC 
AACTGCTTTG ACTTTGGGGT CACAGTGGGC AGCATCTTTG AAGTAGTCTG GGCCATCTTC 
AGACCTGGTA CATCTTTCGG AATCAGTGTC CTGCGAGCTC TCCGGCTTCT AAGAATATTT 
AAAATAACCA AGTATTGGGC ATCCCTCCGG AATTTGGTGG TCTCCTTGAT GAGTTCCATG 
AAGTCTATCA TCAGTTTACT CTTCCTCCTT TTCCTCTTCA TTGTTGTCTT TGCTCTGCTA 
GGAATGCAGC TGTTTGGGGG CAGGTTCAAC TTTAATGATG GGACTCCTTC CGCAAACTTT 
GACACCTTCC CTGCAGCCAT CATGACTGTA TTCCAGATCC TGACGGGTGA GGACTGGAAT 
GAGGTGATGT ACAACGGGAT CCGCTCCCAG GGTGGGGTCA GCTCGGGCAT GTGGTCTGCG 
ATCTATTTCA TTGTGCTTAC CTTGTTTGGA AACTACACAC TGCTGAATGT GTTCTTGGCC 
ATCGCTGTGG ACAATCTGGC CAACGCCCAG GAGCTGACCA AGGATGAACA AGAAGAAGAG 
GAGGCCTTCA ACCAGAAGCA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA 
CCCAACATGC CTTCCATCGA AAGAGACAGA AGGAGAAGAC ACCACATGTC GATGTGGGAG 
CCACGCAGCA GCCACCTGAG GGAGCGGAGG CGCCGGCATC ACATGTCCGT GTGGGAGCAG 
CGAACCAGCC AGTTGAGGAG GCACATGCAG ATGTCCAGCC AGGAGGCCCT CAACAAGGAG 
GAGGCCCCGC CCATGAACCC CCTCAACCCC CTCAACCCGC TGAGCCCCCT CAACCCGCTC 
AGCGCGCACC CCAGCCTCTA CCGGAGACCC CGGGCCATCG AGGGGCTAGC CCTGGGCCTG 
GGGCTGGAGA AGTGTGAGGA AGAGCGCGTC AGCCGTGGGG GGTCCCTCAA AGGGGATGGA 
GGGGACTTGG CGAGCGCCCT GGACAGCCAG AGGAGCCCCC TGGCCCTGGG CAAACGGGAG 
CCACCCTGGC TGGCCCGGCC CTGTCACGGG AACTGTGACC CGACCCAGCC GGAGGCGGGC 
AGTGGGGAGA CGGTGGTGAC CTTCGGGGAC CGGGCCCGGC ACAGGCAGAG CCAGCGGCGC 
AGCCGGCACC GCCGCGTCAG GACCGAGGGC AGGGAGCCCT CCTCCACCTC CAGAAGCAGG 
TCTGCCAGCC AGGAGCGGAG TCTGGACGAA GCTGTGCCCA GCGAGGGGGA GAGGGACCGT 
GAGCCCAGGG CTGGCCATGG GGGCAAAGAA CCCATGATCC AGGAGGAAGA GCGAGAGCGA 
TGCCAGGACT TACGGAGGAC CAACAGTCTG ATGGTGCCCA GAGGCTCCGG GCTGGCAGGA 
GCCCTGGATG AGGCCGACAC ACCCCTTGTC CCACCCCAGC CGGAGCTGGA AGTGGGGAAG 
GACGCAGCCC TGACGGAGCA GGAGCCGGAG GGCAGCAGTG AGCAGGCTCT GCTGGGGGAC 
GTGAAGCTGG ACATAGGCCG GGTCAGCCAG AGCGAGCCCG ACCTCTCCTG CGTCACGGCC 
AACACGGACA AGGCCACCAC CGAGAGCACC AGCGTCACTG TCACCATCCC CGACGTGGGC 
CCCTTGGTGG ACTCAACCGT GGTGCACATC AGCAACAAGA CTGATGGAGA AGCCAGTCCC 
TTGAAAGAGG CAGAGATCAG AGAGGATGAG GAGGAGGTGG AGAAGAAGCA GCAGAAGAAG 
GAGAAGCGTG AGACGGGCAA GGCCATGGTG CCCCACAGCT CGATGTTCAT CTTCAGCACC 
ACCAACCCGA TCCGCAGGGC CTGCCACTAC ATCGTGAACC TGCGCTACTT CGAGATGTGC 
ATCCTCCTGG TGATCGCAGC CAGCAGCATC GCCCTGGCGG CCGAGGACCC CGTCCTGACC 
AACTCAGAGC GCAACAAAGT GCTGAGGTAT TTTGACTATG TGTTCACTGG CGTGTTCACC 
TTTGAGATGG TTATAAAGAT GATAGACCAG GGCTTGATTC TGCAGGACGG CTCCTACTTC 
CGAGACCTGT GGAACATCCT GGACTTCGTG GTGGTGGTTG GCGCATTGGT GGCCTTTGCT 
CTGGCGAATG CTTTGGGAAC TAACAAGGGC CGGGATATTA AGACCATCAA GTCCCTGAGG 
GTTCTCCGTG TCCTGAGGCC GCTGAAGACC ATCAAGCGCC TGCCCAAGCT CAAGGCTGTC 
TTTGACTGTG TGGTCACCTC CTTGAAAAAC GTCTTCAACA TACTCATTGT CTACAAGCTC 
TTCATGTTCA TCTTTGCTGT CATCGCAGTT CAGCTTTTCA AGGGCAAGTT CTTCTACTGC 
ACGGACAGTT CCAAGGACAC AGAGAAGGAG TGCATAGGCA ACTATGTAGA TCACGAGAAA 
AACAAGATGG AGGTGAAAGG CCGAGAGTGG AAGCGCCATG AATTCCATTA TGACAACATC 
ATCTGGGCTC TGCTGACCCT CTTCACTGTC TCCACAGGGG AAGGGTGGCC TCAGGTCTTG 
CAGCACTCGG TCGATGTGAC AGAGGAAGAC CGAGGCCCAA GCCGCAGCAA CCGGATGGAG 
ATGTCCATCT TTTATGTGGT CTACTTTGTG GTCTTCCCCT TCTTCTTTGT CAACATCTTT 
GTGGCTCTCA TCATCATCAC CTTCCAGGAG CAAGGGGACA AGATGATGGA AGAGTGCAGC 
CTGGAGAAGA ATGAGAGGGC ATGCATCGAC TTCGCCATCA GCGCCAAACC GCTCACCCGC 
TACATGCCCC AGAACAGGCA CACCTTCCAG TACCGCGTCT GGCACTTCGT GGTGTCTCCA 
TCCTTTGAGT ACACCATCAT GGCCATGATC GCCCTGAACA CCGTCGTGCT GATGATGAAG 
TACTACTCTG CTCCCTGTAC CTATGAGCTG GCCCTGAAGT ACCTAAATAT CGCCTTCACC 
ATGGTGTTTT CCCTGGAATG TGTCCTGAAG ATCATTGCAT TTGGCTTCTT GAACTATTTC 
CGAGACACCT GGAATATCTT TGACTTCATC ACTGTGATTG GCAGTATCAC AGAAATTATT 
CTGACGGACA GCAAGCTGGT GAACACTAGT GGCTTCAACA TGAGCTTTCT GAAGCTCTTC 
CGAGCGGCCC GCCTCATCAA GCTGCTGCGT CAGGGCTACA CCATCCGCAT TTTACTGTGG 
ACCTTCGTGC AGTCCTTTAA GGCCCTTCCC TATGTCTGCC TTCTGATCGC CATGCTTTTC 
TTCATCTATG CCATCATTGG GATGCAGGTC TTTGGAAACA TAAAGTTAGA TGAGGAGAGT 
CACATCAACC GGCACAACAA CTTCCGGAGT TTCTTTGGGT CCCTCATGCT GCTCTTCAGG 
AGTGCCACGG GTGAGGCCTG GCAGGAGATA ATGCTGTCGT GTCTCGGGGA GAAGGGCTGT 
GAGCCCGACA CCACTGCGCC ATCGGGGCAG AGCGAGAGCG AGCGCTGTGG CACCGACCTG 
GCCTACGTGT ACTTCGTCTC CTTCATCTTC TTCTGCTCCT TCCTGATGCT CAACCTGTTC 
GTGGCCGTCA TCATGGACAA CTTTGAGTAC CTGACTCGGG ACTCGTCCAT CCTGGGGCCT 
CACCATCTGG ACGAGTTTGT CCGAGTCTGG GCAGAGTATG ACAGAGCAGC ATGTGGCCGC 
ATCCATTACA CTGAGATGTA TGAAATGCTG ACTCTCATGT CACCGCCGCT AGGCCTCGGC 
AAGAGATGTC CCTCCAAGGT GGCATATAAG AGGTTGGTCT TGATGAACAT GCCAGTGGCC 
GAGGACATGA CCGTCCACTT CACTTCCACG CTGATGGCTC TAATCCGGAC AGCTCTGGAC 
ATTAAAATCG CCAAAGGCGG TGCAGACAGG CAGCAGCTAG ACTCAGAGCT CCAGAAGGAG 
ACCCTGGCCA TCTGGCCTCA CCTGTCCCAG AAGATGCTGG ATCTGCTCGT GCCCATGCCC 
AAAGCCTCTG ACCTGACTGT GGGCAAAATC TATGCAGCAA TGATGATCAT GGACTACTAC 
AAACAGAGCA AGGTGAAGAA GCAGAGGCAG CAGCTGGAGG AACAGAAAAA CGCACCCATG 
TTCCAGCGCA TGGAGCCCTC GTCTCTGCCT CAGGAGATCA TCGCTAATGC CAAAGCCCTG 
CCTTATCTCC AGCAGGACCC CGTTCCAGGC CTGAGTGGCC GGAGTGCATA CCCTTCGATG 
AGTCCACTCT CCCCGCAGGA AATATTCCAG TTGGCCTGTA TGGACCCAGC TGATGATGGA 
CAGTTCCAGG AACGACAATC TCTGGTGGTG ACAGACCCCA GCTCCATGAG ACGTTCATTT 
TCCACCATTC GCGATAAGCG TTCAAATTCC TCGTGGCTGG AGGAATTCTC CATGGAGCGA 
AGCAGCGAAA ACACCTACAA GTCCCGGCGC CGGAGTTACC ACTCCTCCCT GAGGCTGTCA 
GCCCACCGTC TGAACTCGGA CTCAGGCCAC AAGTCCGACA CCCACCGCTC AGGGGGCAGA 
GAGCGGGGAC GATCGAAAGA GCGAAAGCAT CTTCTCTCTC CTGACGTCTC CCGCTGCAAC 
TCCGAGGAGC GAGGGACCCA GGCCGACTGG GAGTCCCCAG AGCGCCATCA GTCCCGGTCT 
CCCAGTGAAG GCAGGTCACA GACCCCCAAC AGACAGGGCA CGGGGTCCCT GAGTGAGAGC 
TCCATCCCCT CTGTCTCCGA CTCCAGCACC CCACGACGAA GTCGGCGGCA GCTCCCCCCA 
GTCCCTCCCA AGCCTCGGCC CCTCCTTTCC TACAGCTCTC TGATCAGACA CGCTGGGAGC 
ATCTCCCCAC CAGCTGATGG GAGCGAGGGT GGCTCCCCAC TGACCTCCCA AGCTCTGGAG 
AGCAACGACG CCGGCCTGCC CGAGTCTTCC AACCCCCGGC AGGGCCACCC CTCCCCACAG 
CGCTACATCT CCGAGCCCTA CCTGGCCCTG CACGAAGACT CCCACGCCTC GGACTGCGGC 
GAGGAGGAGA CGCTCACCTT TGAAGCAGCC GTGGCCACCA GCCTGGGCCG GTCCAACACC 
ATCGGCTCGG CCCCGCCCCT GCGGCACAGC TGGCAGATGC CCAATGGGCA CTATCGGCGG 
CGGAGGCGCG GGGGGCCTGG ACCGGGCACG ACGTGCGGAG CCGTCAGCGA CCTCCTGAGT 
GACACGGAAG AAGACGACAA ATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	CACNA1E
Entrez Gene ID	353113
Full Name	calcium voltage-gated channel subunit alpha1 E
Synonyms	caV2.3
General protein information	Preferred Names calcium voltage-gated channel subunit alpha1 E Names voltage-dependent R-type calcium channel subunit alpha-1E voltage-gated calcium channel alpha 1E subunit
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Rattus norvegicus (Norway rat)	Cacna1e	NP_062167.2
Xenopus tropicalis (tropical clawed frog)	cacna1e	XP_002937409.2
Homo sapiens (human)	CACNA1E	NP_001192222.1
Bos taurus (cattle)	CACNA1E	NP_001178052.1
Mus musculus (house mouse)	Cacna1e	NP_033912.2
Gallus gallus (chicken)	CACNA1E	XP_422255.4
Pan troglodytes (chimpanzee)	CACNA1E	XP_003949701.1
	CACNA1E	XP_001110352.2
Canis lupus familiaris (dog)	CACNA1E	XP_547425.4
Danio rerio (zebrafish)	LOC564274	XP_001920777.3

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

CACNA1E cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

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