cacna1e cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	cacna1e
Entrez Gene ID	100488514
Full Name	calcium channel, voltage-dependent, R type, alpha 1E subunit
Synonyms	ca(v)2.3,cav2.3
General protein information	Preferred Names calcium channel, voltage-dependent, R type, alpha 1E subunit Names voltage-dependent R-type calcium channel subunit alpha-1E
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018093598.1	XP_017949087.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X2
XM_031900982.1	XP_031756842.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X5
XM_031900983.1	XP_031756843.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X6
XM_018093597.1	XP_017949086.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X1
XM_018093597.2	XP_017949086.2	voltage-dependent R-type calcium channel subunit alpha-1E isoform X2
XM_031900979.1	XP_031756839.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X1
XM_031900980.1	XP_031756840.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X3
XM_031900981.1	XP_031756841.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X4

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Cacna1e	NP_062167.2
Xenopus tropicalis (tropical clawed frog)	cacna1e	XP_002937409.2
Homo sapiens (human)	CACNA1E	NP_001192222.1
Bos taurus (cattle)	CACNA1E	NP_001178052.1
Mus musculus (house mouse)	Cacna1e	NP_033912.2
Gallus gallus (chicken)	CACNA1E	XP_422255.4
Pan troglodytes (chimpanzee)	CACNA1E	XP_003949701.1
	CACNA1E	XP_001110352.2
Canis lupus familiaris (dog)	CACNA1E	XP_547425.4
Danio rerio (zebrafish)	LOC564274	XP_001920777.3

Gene Ontology

The following cacna1e gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cacna1e cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa44182	XM_018093597.1 Latest version!	Xenopus tropicalis calcium channel, voltage-dependent, R type, alpha 1E subunit (cacna1e), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OXa44183	XM_018093598.1 Latest version!	Xenopus tropicalis calcium channel, voltage-dependent, R type, alpha 1E subunit (cacna1e), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OXa57229	XM_031900979.1 Latest version!	Xenopus tropicalis calcium channel, voltage-dependent, R type, alpha 1E subunit (cacna1e), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OXa57230	XM_018093597.2 Latest version!	Xenopus tropicalis calcium channel, voltage-dependent, R type, alpha 1E subunit (cacna1e), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OXa57231	XM_031900980.1 Latest version!	Xenopus tropicalis calcium channel, voltage-dependent, R type, alpha 1E subunit (cacna1e), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OXa57232	XM_031900981.1 Latest version!	Xenopus tropicalis calcium channel, voltage-dependent, R type, alpha 1E subunit (cacna1e), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OXa57233	XM_031900982.1 Latest version!	Xenopus tropicalis calcium channel, voltage-dependent, R type, alpha 1E subunit (cacna1e), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa57234	XM_031900983.1 Latest version!	Xenopus tropicalis calcium channel, voltage-dependent, R type, alpha 1E subunit (cacna1e), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cacna1e ( XM_018093597.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949086.1 Xenopus tropicalis calcium channel, voltage-dependent, R type, alpha 1E subunit (cacna1e), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa44182
Clone ID Related Accession (Same CDS sequence)	XM_018093597.1
Accession Version	XM_018093597.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCCTTG CCACAATTAT TGCAAACTGC ATTGTCCTGG CTTTGGAGCA GCATCTTCCA 
GGCGATGACA AGACTCCAAT GTCTCGCAGA TTGGAAAAGA CTGAGCCCTA TTTCATTGGG 
ATATTTTGCT TTGAAGCTGG AATTAAAATT GTCGCTCTGG GACTGGTTTT TCACAAAGGT 
TCTTATCTTC GGAATGGCTG GAATGTCATG GACTTTATAG TAGTGCTCAG TGGCATCCTG 
GCCACAGCTG GAACTCACTT CAATACTCAC GTGGACCTGC GTACTTTGCG GGCAGTGCGT 
GTTCTCAGGC CGTTGAAGCT GGTCTCGGGG ATTCCCAGTT TACAGATTGT GTTGAAGTCA 
ATCATGAAGG CGATGGTCCC TCTGCTACAG ATTGGATTGT TGCTCTTCTT TGCCATCCTT 
ATGTTTGCTA TCATCGGCCT TGAGTTTTAC AGTGGAAAGC TACACAAGGC CTGTTACAGG 
AACAACTCTG GAATGGAGCT AGACCCCATG GATCCACCTC ACCCTTGTGG AGTACCCCGC 
TGCCCAGCTG GGTATGAGTG CAAGGAATGG ATCGGACCCA ATGATGGAAT AACACAGTTT 
GACAACATCT TATTTGCGGT GCTGACAGTC TTCCAGTGTA TAACTATGGA GGGCTGGACC 
ACAGTGCTGT ACAATACCAA TGATGCCTTA GGAGCCACAT GGAACTGGCT GTACTTCATT 
CCCCTCATTA TCATCGGATC CTTCTTTGTC CTTAATCTTG TCCTTGGAGT GCTGTCCGGT 
GAATTTGCCA AAGAGAGGGA AAGAGTGGAA AACCGCCGGG CTTTCATGAA GTTGCGCAGG 
CAGCAGCAGA TTGAGAGGGA GTTGAATGGC TATCGGGCAT GGATAGATAA AGCTGAGGAA 
GTCATGTTGG CAGAAGAAAA CAAAAATTCT GGGACCTCTG CTTTAGAAGT TCTTAGGCGA 
GCAACCATTA AACGAAGTAG AACAGAAGCC ATGAACCGGG ACTCTACTGA GGACCATTGT 
GTTGACATAT CCTCTGTTGG CACCCCCCTT GCTAGAGCAA GCATCAAAAG TACCAAAATG 
GATGGGGCAT CATATTTTCG GCACAAGGAA AAACTATTGC GTGTCTCCGT TCGTCACATG 
ATCAAATCCC AGGTTTTCTA CTGGATGGTC CTGGGAGTGG TAGCACTTAA CACTTTCTGT 
GTGGCTATTG TCCATCACAA CCAGACCCTG TGGCTCTCAA ATGTTCTTTA CTATGCAGAA 
TTTCTGTTTC TTGGGTTATT TTTGGTGGAG ATGGCTTTTA AAATGTATGG AATGGGTCCA 
CGTCTTTACT TTCACTCATC CTTCAACTGC TTTGATTGCG GGGTCACAAT AGGTAGTATA 
TTTGAAGTAC TTTGGGCCAT ATTTAGACCA GGAACTTCCT TTGGAATCAG TGTCTTAAGA 
GCTCTCCGAC TTTTGAGAAT CTTCAAAATA ACAAAATACT GGGCCTCACT ACGAAACCTT 
GTAGTATCAT TGATGAATTC TATGAAATCC ATCATCAGCC TGCTCTTCCT TCTATTCCTC 
TTCATAGTTG TTTTTGCTCT CCTTGGAATG CAGTTATATG GTGGCAGATT CAATTTTAAT 
GATGGCACTC CTTCTGCAAA CTTTGATACA TTCCCTGCAG CCATCATGAC TGTATTCCAG 
ATTCTGACAG GAGAGGATTG GAATGAAGTG ATGTACAATG GTATTCGCTC CCAAGGTGGT 
GTTCGATCCG GAATGTGGTC TTCCATATAC TTTATTGTCC TTACCCTTTT TGGAAATTAC 
ACACTGCTAA ATGTATTTCT TGCCATTGCT GTGGACAACC TGGCTAATGC ACAGGAGTTA 
ACTAAGGATG AGCAAGAAGA AGAGGAAGCT TTCAATCAGA AGCATGCACT GCAAAAAGCG 
AAGGAGGTCA GCCCCATGTC TGCACCTAAT GCATCATGTA TTGAGAAGGA GCGCAGAAAA 
CGGCACACAT TGTCTATATG GGAGCAGCGC ACAAGCCAGC TAAGGAGGCA CATACAGATG 
TCTAGTCAAG AAGCTCTCCA GAGTGAAGAA CTGGCAGCAT TGAATCCACA CTCCAGTATC 
TATCGCAAGA CAAAGGAAGC TGCTGAACAA AACACAGAAA AAGAAGAAGT GCATCAAGAC 
GCAAAACTGG CAATAGACCC AGTTGAAGGA CCACCAGAAG GTGAAGGAAG CCCTTCTAAT 
AGAAATCTCA GGGAAAACTG GCGCCAGAAG TCCAGGCATG GAAATTCTGA AAACACTGAA 
CCAAATGGAA CAACTCCAGA AGAAGCAATG CGCCGCAGAC AAAGTCAGAG ACGCAGCCGT 
CATAGAAGAG TTAGAACTGA AGGTCGTGAG CAGTGTAGCA ATCCAAACAG TACCTCAGCA 
AGCAGAGAAG GTGGTCTTGA CAAAGTCTCT GCAGAGGATG GAAGAACAAT TCCAGAAGAG 
AAGAGCAATG GAGAAGCCAA AGAAGTCAAA GGGGTTGATG ATGAGCCTCT AAAGATTTGT 
ATATCTTCAA GAAATTGCGA GGACAGAAAT ATCAGGACTT TGGGTTGTGA GGGTATTGCC 
TTGAACGATA CACCCCTGCA GGATGTGAAC AGCCAAGAGA AGGACAGTAG CCCTCCTGAT 
TTGGATTGCA GTGAGCAGGC TCTGTTGGGC AGCATTCAGA GCCAGGAAAG CCCTAATCCA 
ACCAGCTGCA GTTTGCCAGA TGTGTCCAGC AGCACCGAGA GAGTCACAGA GAGCACCGTC 
ATTACCTTAC CAGATACTGG AATGCCCACC GACTGCACCG TGGTACAGAT GAGTGAAGGC 
AGGGATGGAG AAGTCAACTT TGGGTCTTCG CAGCAAGAAA AAGAAGAGGA GGAAGCTGAG 
GATGGAGAAG AAAGAACTAT AAAAAGAAAA GAGAAATTGT TGGAGTCCCC CAAAGCCATT 
GTACCGTACA GTTCAATGCT CATCTTCAGC TCCACCAATC CCATAAGAAA AGTATGTCAT 
TACATTGTTA ACCTACGATA CTTTGAGATG AGCATCCTGC TGGTAATAGC AGCAAGTAGT 
ATAGCCTTAG CAGCGGAGGA CCCTGTACTG ACCAACTCGG ACCGCAACAA AGTTCTTCGC 
TATTTTGACT ATGTTTTCAC AGGGGTCTTC ACTTTTGAGA TGATTATAAA GATGATTGAC 
CAAGGTTTGA TTTTACACGA GGGCTCCTAC TTTCGTGACC TATGGAACAT CTTGGATTTT 
GTTGTTGTTG TGGGAGCCTT AATTGCGTTT GCCTTAGCGA ATGTTTTGGG GACTAACAAA 
GGCCGGGACA TCAAAACCAT TAAGTCTCTA CGTGTGTTAC GGGTGCTGCG TCCACTAAAA 
ACAATCAAAC GCCTGCCAAA ACTGAAGGCA GTCTTTGATT GTGTGGTTAA CTCTTTAAAG 
AATGTCTTTA ATATTCTCAT CGTATACAAA CTCTTCATGT TCATATTTGC TGTAATCGCT 
GTCCAGCTGT TTAAGGGCAA GTTCTTCTAT TGTACAGACA GCTCAAAGGA GACAGAGAAA 
GAGTGCATAG GGAGTTACAT CGATCACGAA AAGAACAAGA TGGAGGTGAA GCGGAGAGAG 
TGGAAAAGAC ATGATTTCCA TTATGACAAC ATAATATGGG CTCTTCTTAC CCTCTTTACT 
GTATCAACAG GGGAAGGCTG GCCTCAGGTT CTGCAGCACT CTGTTGATAT AACTGAAGAA 
GATAGGGGTC CAAGCCGAAG CAACCGTATG GAGATGTCCA TTTTCTATGT TATCTACTTT 
GTGGTCTTCC CCTTCTTCTT TGTAAATATA TTTGTGGCTC TGATTATCAT CACATTCCAG 
GAGCAAGGAG ACAAGATGAT GGAGGAATGT TCCCTAGAAA AGAATGAGAG AGCCTGCATA 
GACTTTGCAA TAAGTGCTAA GCCACTTACA CGATATATGC CACAGAACAG GCACACATTC 
CAGTACCGGG TCTGGCACTT CGTGGTCTCC CCATCATTTG AATACACTAT CATGGCTATG 
ATTGCACTAA ACACAGTGGT ACTCATGATG AAGTATTATT CTGCGCCATA CACATATGAG 
CTTGCATTAA AGTATCTCAA TATTGCATTC ACAATGCTAT TTTCTCTGGA GTGCATCCTG 
AAAATAATTG CATTTGGGTT TTTGAATTAC TTTCGGGATA CTTGGAACAT TTTTGACTTC 
ATTACTGTAA TTGGTAGCAT CACGGAAATT ATTCTAACAG ATAGTAAGTT GGTGAACACC 
AGCAGCTTTA ATATGAGCTT TCTGAAGCTG TTCCGAGCGG CGCGACTTAT TAAACTTCTC 
CGGCAAGGGT ACACCATCCG GATCCTGCTG TGGACTTTTG TTCAGTCCTT TAAGGCACTT 
CCTTATGTGT GTCTTCTCAT TGCAATGTTG TTCTTCATCT ATGCCATTAT TGGGATGCAG 
GTGTTTGGCA ATATCCGCCT GGAAGAGGAC AGTCATATTA ATAGGCACAA CAATTTTAGA 
AGCTTCTTTA GTTCTCTTAT GTTACTCTTC AGGAGTGCCA CAGGAGAAGC GTGGCAGGAG 
ATCATGCTTT CATGCCTTAG TGATAAGGGT TGTGAGCCAG ACACCAGTGC AGCCTCTGGA 
CACAATGAGA ATGAGCGCTG TGGAACTGAA CTTGCATATG TTTATTTTGT TTCATTCATT 
TTCTTCTGCT CCTTCCTCAT GCTAAATCTG TTTGTGGCTG TCATTATGGA CAACTTTGAG 
TACTTGACAC GGGACTCTTC CATTTTGGGA CCTCATCACT TGGATGAGTT TGTTCGGATA 
TGGGCTGAAT ATGATCGAGC AGCATGTGGC AGGATCCCCT ATAAGGATAT GTACAAATTA 
GTCCGAGTCA TCTCGCCTCC GCTCGGCCTA GGAGAGAACT GCCCATACAG GGTGGCATGC 
AAGAGATTGG TCCTGATGAA TATGCCAGTG TCGGAGGACA TGACGGTTCA TTTCACATCA 
ACTCTTATGG CCCTAATACG CACGGCACTG GATATCAAAA TAGCTAAAGG AGGTGCTGAC 
AGGCAACAGC TGGACTCAGA GCTGCAGAAA GATATACTTG CCATTTGGCC AAATCTCTCC 
CAGAAAATGA TCGACTTACT TGTGCCAATG CCAAGAGCCT CTGATCTGAC GGTGGGGAAG 
ATTTACGCTG CAATGATGAT CATGGATTAC TATAAACAGA GCAAAGCGAA AAAACAAAGG 
CAGCAGCTGG AGGAGCAGAA AAATGCACCT ATGTTTCAGA GAATGGAGGC TTCTGCCCTC 
CCTGAGGAGA TCTTATCCAA TGCCAAAGCT TTACCATACC TTCAGCAAGA CACTCTATCA 
GGCCTGGGTG GATTTCCTTC ACTGAGTCCC TTGTCCCCAG AAGAAGTATT CCAGCCGCCA 
TGCCTAGACG GGGAAGAGGA GGAAGACTTT GAAGAGCAGC TGTCTCTGGA GCCTGAGGTT 
AGAGAGTTTA GAAGGGAGCA GCTCCCTAAC CGAGGCAGCT ACATCCCTGT GGAGATGCTG 
GAACGTCCAG CGTCTGTGAG AGCTTCTTCC ATGCCACGTC TGGCTGAGAA TCCTCAGGTC 
CTGTCAGACG TAAGCTCCAT GCGTCGCTCC TTCTCCACAA CTGCAGAGAA GCGAGTGCGC 
AGCTCGTGGC TAGATGAGTT CACTATGGAC AGGAGTAGCA CTGACTGTGC ATACAGATAC 
CAGAGGCGCA GTTATCATGC ATCCTTAAGA CTGTCTGCAC ATCGCCTGAA TACAGATACA 
GGTCACTGGT CAGATAATCA CCGTTCTGGC GGAAGGGAGC GAGGAAGGTC CAAAGAGAGA 
AAACACTTGC TGTCACCCGA TCCTTCCCGC TGTAATTCTG AGGAAAGGAG CCCACAAGCT 
GCCTGTGAAC ACCGAGATCA CCAACTGTCC CCTGGGCATA CTCCCAGTAG TGTACAGATT 
CTTGACTCTC TGAGTGAAAG TTGTACCCCT TCAGACACCA GCACCCCAAG AAGAGGCCGT 
AGACAGCTTC CTTCTGTGCC TCCAACTCCT AGACCCCTTA TCTCTTTCTC CTCGTTAATC 
CGCCGGCAGA AACACAGCCC CCCTCCTCAA AATAAAAATG ATACTATCCT TCCTAGTGAG 
CCTGGTCTGA CTGAGTCTTC CTGCTCCCTT GGGGGTCATC AGAGCTCCCA GTCCACACCA 
CAGCATTATA TCTCAGAGCC CTACCTGGCA CTGCATGAGG AGATGCAAAG CTCAGACTGC 
CCAGCAGAGG ACACACTCAC CTTTGAGGTT GCTGTGGCTA CTAGTCTGGG CCGCTCGAAC 
ACCATTGGCT CTGCCCCTCT ACGTCATGGC TGGCAAATGC CCAATGGTCA CTACCGGCGT 
CGAAGGAAGG GAGGAATTCC ACCAGTGATA CCCTCCAGAG AGTTAAGTGA CACAGAAGAG 
GATGATCGAT GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949086.1
CDS	9..6443
Translation	MILATIIANCIVLALEQHLPGDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGLVFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHKACYRNNSGMELDPMDPPHPCGVPRCPAGYECKEWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNSGTSALEVLRRATIKRSRTEAMNRDSTEDHCVDISSVGTPLARASIKSTKMDGASYFRHKEKLLRVSVRHMIKSQVFYWMVLGVVALNTFCVAIVHHNQTLWLSNVLYYAEFLFLGLFLVEMAFKMYGMGPRLYFHSSFNCFDCGVTIGSIFEVLWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMNSMKSIISLLFLLFLFIVVFALLGMQLYGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVRSGMWSSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNASCIEKERRKRHTLSIWEQRTSQLRRHIQMSSQEALQSEELAALNPHSSIYRKTKEAAEQNTEKEEVHQDAKLAIDPVEGPPEGEGSPSNRNLRENWRQKSRHGNSENTEPNGTTPEEAMRRRQSQRRSRHRRVRTEGREQCSNPNSTSASREGGLDKVSAEDGRTIPEEKSNGEAKEVKGVDDEPLKICISSRNCEDRNIRTLGCEGIALNDTPLQDVNSQEKDSSPPDLDCSEQALLGSIQSQESPNPTSCSLPDVSSSTERVTESTVITLPDTGMPTDCTVVQMSEGRDGEVNFGSSQQEKEEEEAEDGEERTIKRKEKLLESPKAIVPYSSMLIFSSTNPIRKVCHYIVNLRYFEMSILLVIAASSIALAAEDPVLTNSDRNKVLRYFDYVFTGVFTFEMIIKMIDQGLILHEGSYFRDLWNILDFVVVVGALIAFALANVLGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKETEKECIGSYIDHEKNKMEVKRREWKRHDFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDITEEDRGPSRSNRMEMSIFYVIYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPYTYELALKYLNIAFTMLFSLECILKIIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSSFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIRLEEDSHINRHNNFRSFFSSLMLLFRSATGEAWQEIMLSCLSDKGCEPDTSAASGHNENERCGTELAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRIWAEYDRAACGRIPYKDMYKLVRVISPPLGLGENCPYRVACKRLVLMNMPVSEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKDILAIWPNLSQKMIDLLVPMPRASDLTVGKIYAAMMIMDYYKQSKAKKQRQQLEEQKNAPMFQRMEASALPEEILSNAKALPYLQQDTLSGLGGFPSLSPLSPEEVFQPPCLDGEEEEDFEEQLSLEPEVREFRREQLPNRGSYIPVEMLERPASVRASSMPRLAENPQVLSDVSSMRRSFSTTAEKRVRSSWLDEFTMDRSSTDCAYRYQRRSYHASLRLSAHRLNTDTGHWSDNHRSGGRERGRSKERKHLLSPDPSRCNSEERSPQAACEHRDHQLSPGHTPSSVQILDSLSESCTPSDTSTPRRGRRQLPSVPPTPRPLISFSSLIRRQKHSPPPQNKNDTILPSEPGLTESSCSLGGHQSSQSTPQHYISEPYLALHEEMQSSDCPAEDTLTFEVAVATSLGRSNTIGSAPLRHGWQMPNGHYRRRRKGGIPPVIPSRELSDTEEDDRC

Target ORF information:

RefSeq Version	XM_018093597.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calcium channel, voltage-dependent, R type, alpha 1E subunit (cacna1e), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018093597.1

ORF Insert Sequence:

ATGATCCTTG CCACAATTAT TGCAAACTGC ATTGTCCTGG CTTTGGAGCA GCATCTTCCA 
GGCGATGACA AGACTCCAAT GTCTCGCAGA TTGGAAAAGA CTGAGCCCTA TTTCATTGGG 
ATATTTTGCT TTGAAGCTGG AATTAAAATT GTCGCTCTGG GACTGGTTTT TCACAAAGGT 
TCTTATCTTC GGAATGGCTG GAATGTCATG GACTTTATAG TAGTGCTCAG TGGCATCCTG 
GCCACAGCTG GAACTCACTT CAATACTCAC GTGGACCTGC GTACTTTGCG GGCAGTGCGT 
GTTCTCAGGC CGTTGAAGCT GGTCTCGGGG ATTCCCAGTT TACAGATTGT GTTGAAGTCA 
ATCATGAAGG CGATGGTCCC TCTGCTACAG ATTGGATTGT TGCTCTTCTT TGCCATCCTT 
ATGTTTGCTA TCATCGGCCT TGAGTTTTAC AGTGGAAAGC TACACAAGGC CTGTTACAGG 
AACAACTCTG GAATGGAGCT AGACCCCATG GATCCACCTC ACCCTTGTGG AGTACCCCGC 
TGCCCAGCTG GGTATGAGTG CAAGGAATGG ATCGGACCCA ATGATGGAAT AACACAGTTT 
GACAACATCT TATTTGCGGT GCTGACAGTC TTCCAGTGTA TAACTATGGA GGGCTGGACC 
ACAGTGCTGT ACAATACCAA TGATGCCTTA GGAGCCACAT GGAACTGGCT GTACTTCATT 
CCCCTCATTA TCATCGGATC CTTCTTTGTC CTTAATCTTG TCCTTGGAGT GCTGTCCGGT 
GAATTTGCCA AAGAGAGGGA AAGAGTGGAA AACCGCCGGG CTTTCATGAA GTTGCGCAGG 
CAGCAGCAGA TTGAGAGGGA GTTGAATGGC TATCGGGCAT GGATAGATAA AGCTGAGGAA 
GTCATGTTGG CAGAAGAAAA CAAAAATTCT GGGACCTCTG CTTTAGAAGT TCTTAGGCGA 
GCAACCATTA AACGAAGTAG AACAGAAGCC ATGAACCGGG ACTCTACTGA GGACCATTGT 
GTTGACATAT CCTCTGTTGG CACCCCCCTT GCTAGAGCAA GCATCAAAAG TACCAAAATG 
GATGGGGCAT CATATTTTCG GCACAAGGAA AAACTATTGC GTGTCTCCGT TCGTCACATG 
ATCAAATCCC AGGTTTTCTA CTGGATGGTC CTGGGAGTGG TAGCACTTAA CACTTTCTGT 
GTGGCTATTG TCCATCACAA CCAGACCCTG TGGCTCTCAA ATGTTCTTTA CTATGCAGAA 
TTTCTGTTTC TTGGGTTATT TTTGGTGGAG ATGGCTTTTA AAATGTATGG AATGGGTCCA 
CGTCTTTACT TTCACTCATC CTTCAACTGC TTTGATTGCG GGGTCACAAT AGGTAGTATA 
TTTGAAGTAC TTTGGGCCAT ATTTAGACCA GGAACTTCCT TTGGAATCAG TGTCTTAAGA 
GCTCTCCGAC TTTTGAGAAT CTTCAAAATA ACAAAATACT GGGCCTCACT ACGAAACCTT 
GTAGTATCAT TGATGAATTC TATGAAATCC ATCATCAGCC TGCTCTTCCT TCTATTCCTC 
TTCATAGTTG TTTTTGCTCT CCTTGGAATG CAGTTATATG GTGGCAGATT CAATTTTAAT 
GATGGCACTC CTTCTGCAAA CTTTGATACA TTCCCTGCAG CCATCATGAC TGTATTCCAG 
ATTCTGACAG GAGAGGATTG GAATGAAGTG ATGTACAATG GTATTCGCTC CCAAGGTGGT 
GTTCGATCCG GAATGTGGTC TTCCATATAC TTTATTGTCC TTACCCTTTT TGGAAATTAC 
ACACTGCTAA ATGTATTTCT TGCCATTGCT GTGGACAACC TGGCTAATGC ACAGGAGTTA 
ACTAAGGATG AGCAAGAAGA AGAGGAAGCT TTCAATCAGA AGCATGCACT GCAAAAAGCG 
AAGGAGGTCA GCCCCATGTC TGCACCTAAT GCATCATGTA TTGAGAAGGA GCGCAGAAAA 
CGGCACACAT TGTCTATATG GGAGCAGCGC ACAAGCCAGC TAAGGAGGCA CATACAGATG 
TCTAGTCAAG AAGCTCTCCA GAGTGAAGAA CTGGCAGCAT TGAATCCACA CTCCAGTATC 
TATCGCAAGA CAAAGGAAGC TGCTGAACAA AACACAGAAA AAGAAGAAGT GCATCAAGAC 
GCAAAACTGG CAATAGACCC AGTTGAAGGA CCACCAGAAG GTGAAGGAAG CCCTTCTAAT 
AGAAATCTCA GGGAAAACTG GCGCCAGAAG TCCAGGCATG GAAATTCTGA AAACACTGAA 
CCAAATGGAA CAACTCCAGA AGAAGCAATG CGCCGCAGAC AAAGTCAGAG ACGCAGCCGT 
CATAGAAGAG TTAGAACTGA AGGTCGTGAG CAGTGTAGCA ATCCAAACAG TACCTCAGCA 
AGCAGAGAAG GTGGTCTTGA CAAAGTCTCT GCAGAGGATG GAAGAACAAT TCCAGAAGAG 
AAGAGCAATG GAGAAGCCAA AGAAGTCAAA GGGGTTGATG ATGAGCCTCT AAAGATTTGT 
ATATCTTCAA GAAATTGCGA GGACAGAAAT ATCAGGACTT TGGGTTGTGA GGGTATTGCC 
TTGAACGATA CACCCCTGCA GGATGTGAAC AGCCAAGAGA AGGACAGTAG CCCTCCTGAT 
TTGGATTGCA GTGAGCAGGC TCTGTTGGGC AGCATTCAGA GCCAGGAAAG CCCTAATCCA 
ACCAGCTGCA GTTTGCCAGA TGTGTCCAGC AGCACCGAGA GAGTCACAGA GAGCACCGTC 
ATTACCTTAC CAGATACTGG AATGCCCACC GACTGCACCG TGGTACAGAT GAGTGAAGGC 
AGGGATGGAG AAGTCAACTT TGGGTCTTCG CAGCAAGAAA AAGAAGAGGA GGAAGCTGAG 
GATGGAGAAG AAAGAACTAT AAAAAGAAAA GAGAAATTGT TGGAGTCCCC CAAAGCCATT 
GTACCGTACA GTTCAATGCT CATCTTCAGC TCCACCAATC CCATAAGAAA AGTATGTCAT 
TACATTGTTA ACCTACGATA CTTTGAGATG AGCATCCTGC TGGTAATAGC AGCAAGTAGT 
ATAGCCTTAG CAGCGGAGGA CCCTGTACTG ACCAACTCGG ACCGCAACAA AGTTCTTCGC 
TATTTTGACT ATGTTTTCAC AGGGGTCTTC ACTTTTGAGA TGATTATAAA GATGATTGAC 
CAAGGTTTGA TTTTACACGA GGGCTCCTAC TTTCGTGACC TATGGAACAT CTTGGATTTT 
GTTGTTGTTG TGGGAGCCTT AATTGCGTTT GCCTTAGCGA ATGTTTTGGG GACTAACAAA 
GGCCGGGACA TCAAAACCAT TAAGTCTCTA CGTGTGTTAC GGGTGCTGCG TCCACTAAAA 
ACAATCAAAC GCCTGCCAAA ACTGAAGGCA GTCTTTGATT GTGTGGTTAA CTCTTTAAAG 
AATGTCTTTA ATATTCTCAT CGTATACAAA CTCTTCATGT TCATATTTGC TGTAATCGCT 
GTCCAGCTGT TTAAGGGCAA GTTCTTCTAT TGTACAGACA GCTCAAAGGA GACAGAGAAA 
GAGTGCATAG GGAGTTACAT CGATCACGAA AAGAACAAGA TGGAGGTGAA GCGGAGAGAG 
TGGAAAAGAC ATGATTTCCA TTATGACAAC ATAATATGGG CTCTTCTTAC CCTCTTTACT 
GTATCAACAG GGGAAGGCTG GCCTCAGGTT CTGCAGCACT CTGTTGATAT AACTGAAGAA 
GATAGGGGTC CAAGCCGAAG CAACCGTATG GAGATGTCCA TTTTCTATGT TATCTACTTT 
GTGGTCTTCC CCTTCTTCTT TGTAAATATA TTTGTGGCTC TGATTATCAT CACATTCCAG 
GAGCAAGGAG ACAAGATGAT GGAGGAATGT TCCCTAGAAA AGAATGAGAG AGCCTGCATA 
GACTTTGCAA TAAGTGCTAA GCCACTTACA CGATATATGC CACAGAACAG GCACACATTC 
CAGTACCGGG TCTGGCACTT CGTGGTCTCC CCATCATTTG AATACACTAT CATGGCTATG 
ATTGCACTAA ACACAGTGGT ACTCATGATG AAGTATTATT CTGCGCCATA CACATATGAG 
CTTGCATTAA AGTATCTCAA TATTGCATTC ACAATGCTAT TTTCTCTGGA GTGCATCCTG 
AAAATAATTG CATTTGGGTT TTTGAATTAC TTTCGGGATA CTTGGAACAT TTTTGACTTC 
ATTACTGTAA TTGGTAGCAT CACGGAAATT ATTCTAACAG ATAGTAAGTT GGTGAACACC 
AGCAGCTTTA ATATGAGCTT TCTGAAGCTG TTCCGAGCGG CGCGACTTAT TAAACTTCTC 
CGGCAAGGGT ACACCATCCG GATCCTGCTG TGGACTTTTG TTCAGTCCTT TAAGGCACTT 
CCTTATGTGT GTCTTCTCAT TGCAATGTTG TTCTTCATCT ATGCCATTAT TGGGATGCAG 
GTGTTTGGCA ATATCCGCCT GGAAGAGGAC AGTCATATTA ATAGGCACAA CAATTTTAGA 
AGCTTCTTTA GTTCTCTTAT GTTACTCTTC AGGAGTGCCA CAGGAGAAGC GTGGCAGGAG 
ATCATGCTTT CATGCCTTAG TGATAAGGGT TGTGAGCCAG ACACCAGTGC AGCCTCTGGA 
CACAATGAGA ATGAGCGCTG TGGAACTGAA CTTGCATATG TTTATTTTGT TTCATTCATT 
TTCTTCTGCT CCTTCCTCAT GCTAAATCTG TTTGTGGCTG TCATTATGGA CAACTTTGAG 
TACTTGACAC GGGACTCTTC CATTTTGGGA CCTCATCACT TGGATGAGTT TGTTCGGATA 
TGGGCTGAAT ATGATCGAGC AGCATGTGGC AGGATCCCCT ATAAGGATAT GTACAAATTA 
GTCCGAGTCA TCTCGCCTCC GCTCGGCCTA GGAGAGAACT GCCCATACAG GGTGGCATGC 
AAGAGATTGG TCCTGATGAA TATGCCAGTG TCGGAGGACA TGACGGTTCA TTTCACATCA 
ACTCTTATGG CCCTAATACG CACGGCACTG GATATCAAAA TAGCTAAAGG AGGTGCTGAC 
AGGCAACAGC TGGACTCAGA GCTGCAGAAA GATATACTTG CCATTTGGCC AAATCTCTCC 
CAGAAAATGA TCGACTTACT TGTGCCAATG CCAAGAGCCT CTGATCTGAC GGTGGGGAAG 
ATTTACGCTG CAATGATGAT CATGGATTAC TATAAACAGA GCAAAGCGAA AAAACAAAGG 
CAGCAGCTGG AGGAGCAGAA AAATGCACCT ATGTTTCAGA GAATGGAGGC TTCTGCCCTC 
CCTGAGGAGA TCTTATCCAA TGCCAAAGCT TTACCATACC TTCAGCAAGA CACTCTATCA 
GGCCTGGGTG GATTTCCTTC ACTGAGTCCC TTGTCCCCAG AAGAAGTATT CCAGCCGCCA 
TGCCTAGACG GGGAAGAGGA GGAAGACTTT GAAGAGCAGC TGTCTCTGGA GCCTGAGGTT 
AGAGAGTTTA GAAGGGAGCA GCTCCCTAAC CGAGGCAGCT ACATCCCTGT GGAGATGCTG 
GAACGTCCAG CGTCTGTGAG AGCTTCTTCC ATGCCACGTC TGGCTGAGAA TCCTCAGGTC 
CTGTCAGACG TAAGCTCCAT GCGTCGCTCC TTCTCCACAA CTGCAGAGAA GCGAGTGCGC 
AGCTCGTGGC TAGATGAGTT CACTATGGAC AGGAGTAGCA CTGACTGTGC ATACAGATAC 
CAGAGGCGCA GTTATCATGC ATCCTTAAGA CTGTCTGCAC ATCGCCTGAA TACAGATACA 
GGTCACTGGT CAGATAATCA CCGTTCTGGC GGAAGGGAGC GAGGAAGGTC CAAAGAGAGA 
AAACACTTGC TGTCACCCGA TCCTTCCCGC TGTAATTCTG AGGAAAGGAG CCCACAAGCT 
GCCTGTGAAC ACCGAGATCA CCAACTGTCC CCTGGGCATA CTCCCAGTAG TGTACAGATT 
CTTGACTCTC TGAGTGAAAG TTGTACCCCT TCAGACACCA GCACCCCAAG AAGAGGCCGT 
AGACAGCTTC CTTCTGTGCC TCCAACTCCT AGACCCCTTA TCTCTTTCTC CTCGTTAATC 
CGCCGGCAGA AACACAGCCC CCCTCCTCAA AATAAAAATG ATACTATCCT TCCTAGTGAG 
CCTGGTCTGA CTGAGTCTTC CTGCTCCCTT GGGGGTCATC AGAGCTCCCA GTCCACACCA 
CAGCATTATA TCTCAGAGCC CTACCTGGCA CTGCATGAGG AGATGCAAAG CTCAGACTGC 
CCAGCAGAGG ACACACTCAC CTTTGAGGTT GCTGTGGCTA CTAGTCTGGG CCGCTCGAAC 
ACCATTGGCT CTGCCCCTCT ACGTCATGGC TGGCAAATGC CCAATGGTCA CTACCGGCGT 
CGAAGGAAGG GAGGAATTCC ACCAGTGATA CCCTCCAGAG AGTTAAGTGA CACAGAAGAG 
GATGATCGAT GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1e ( XM_018093598.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949087.1 Xenopus tropicalis calcium channel, voltage-dependent, R type, alpha 1E subunit (cacna1e), transcript variant X2, mRNA.

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CloneID	OXa44183
Clone ID Related Accession (Same CDS sequence)	XM_018093598.1
Accession Version	XM_018093598.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCCTTG CCACAATTAT TGCAAACTGC ATTGTCCTGG CTTTGGAGCA GCATCTTCCA 
GGCGATGACA AGACTCCAAT GTCTCGCAGA TTGGAAAAGA CTGAGCCCTA TTTCATTGGG 
ATATTTTGCT TTGAAGCTGG AATTAAAATT GTCGCTCTGG GACTGGTTTT TCACAAAGGT 
TCTTATCTTC GGAATGGCTG GAATGTCATG GACTTTATAG TAGTGCTCAG TGGCATCCTG 
GCCACAGCTG GAACTCACTT CAATACTCAC GTGGACCTGC GTACTTTGCG GGCAGTGCGT 
GTTCTCAGGC CGTTGAAGCT GGTCTCGGGG ATTCCCAGTT TACAGATTGT GTTGAAGTCA 
ATCATGAAGG CGATGGTCCC TCTGCTACAG ATTGGATTGT TGCTCTTCTT TGCCATCCTT 
ATGTTTGCTA TCATCGGCCT TGAGTTTTAC AGTGGAAAGC TACACAAGGC CTGTTACAGG 
AACAACTCTG GAATGGAGCT AGACCCCATG GATCCACCTC ACCCTTGTGG AGTACCCCGC 
TGCCCAGCTG GGTATGAGTG CAAGGAATGG ATCGGACCCA ATGATGGAAT AACACAGTTT 
GACAACATCT TATTTGCGGT GCTGACAGTC TTCCAGTGTA TAACTATGGA GGGCTGGACC 
ACAGTGCTGT ACAATACCAA TGATGCCTTA GGAGCCACAT GGAACTGGCT GTACTTCATT 
CCCCTCATTA TCATCGGATC CTTCTTTGTC CTTAATCTTG TCCTTGGAGT GCTGTCCGGT 
GAATTTGCCA AAGAGAGGGA AAGAGTGGAA AACCGCCGGG CTTTCATGAA GTTGCGCAGG 
CAGCAGCAGA TTGAGAGGGA GTTGAATGGC TATCGGGCAT GGATAGATAA AGCTGAGGAA 
GTCATGTTGG CAGAAGAAAA CAAAAATTCT GGGACCTCTG CTTTAGAAGT TCTTAGGCGA 
GCAACCATTA AACGAAGTAG AACAGAAGCC ATGAACCGGG ACTCTACTGA GGACCATTGT 
GTTGACATAT CCTCTGTTGG CACCCCCCTT GCTAGAGCAA GCATCAAAAG TACCAAAATG 
GATGGGGCAT CATATTTTCG GCACAAGGAA AAACTATTGC GTGTCTCCGT TCGTCACATG 
ATCAAATCCC AGGTTTTCTA CTGGATGGTC CTGGGAGTGG TAGCACTTAA CACTTTCTGT 
GTGGCTATTG TCCATCACAA CCAGACCCTG TGGCTCTCAA ATGTTCTTTA CTATGCAGAA 
TTTCTGTTTC TTGGGTTATT TTTGGTGGAG ATGGCTTTTA AAATGTATGG AATGGGTCCA 
CGTCTTTACT TTCACTCATC CTTCAACTGC TTTGATTGCG GGGTCACAAT AGGTAGTATA 
TTTGAAGTAC TTTGGGCCAT ATTTAGACCA GGAACTTCCT TTGGAATCAG TGTCTTAAGA 
GCTCTCCGAC TTTTGAGAAT CTTCAAAATA ACAAAATACT GGGCCTCACT ACGAAACCTT 
GTAGTATCAT TGATGAATTC TATGAAATCC ATCATCAGCC TGCTCTTCCT TCTATTCCTC 
TTCATAGTTG TTTTTGCTCT CCTTGGAATG CAGTTATATG GTGGCAGATT CAATTTTAAT 
GATGGCACTC CTTCTGCAAA CTTTGATACA TTCCCTGCAG CCATCATGAC TGTATTCCAG 
ATTCTGACAG GAGAGGATTG GAATGAAGTG ATGTACAATG GTATTCGCTC CCAAGGTGGT 
GTTCGATCCG GAATGTGGTC TTCCATATAC TTTATTGTCC TTACCCTTTT TGGAAATTAC 
ACACTGCTAA ATGTATTTCT TGCCATTGCT GTGGACAACC TGGCTAATGC ACAGGAGTTA 
ACTAAGGATG AGCAAGAAGA AGAGGAAGCT TTCAATCAGA AGCATGCACT GCAAAAAGCG 
AAGGAGGTCA GCCCCATGTC TGCACCTAAT GCATCATGTA TTGAGAAGGA GCGCAGAAAA 
CGGCACACAT TGTCTATATG GGAGCAGCGC ACAAGCCAGC TAAGGAGGCA CATACAGATG 
TCTAGTCAAG AAGCTCTCCA GAGTGAAGAA CTGGCAGCAT TGAATCCACA CTCCAGTATC 
TATCGCAAGA CAAAGGAAGC TGCTGAACAA AACACAGAAA AAGAAGAAGT GCATCAAGAC 
GCAAAACTGG CAATAGACCC AGTTGAAGGA CCACCAGAAG GTGAAGGAAG CCCTTCTAAT 
AGAAATCTCA GGGAAAACTG GCGCCAGAAG TCCAGGCATG GAAATTCTGA AAACACTGAA 
CCAAATGGAA CAACTCCAGA AGAAGCAATG CGCCGCAGAC AAAGTCAGAG ACGCAGCCGT 
CATAGAAGAG TTAGAACTGA AGGTCGTGAG CAGTGTAGCA ATCCAAACAG TACCTCAGCA 
AGCAGAGAAG GTGGTCTTGA CAAAGTCTCT GCAGAGGATG GAAGAACAAT TCCAGAAGAG 
AAGAGCAATG GAGAAGCCAA AGAAGTCAAA GGGGTTGATG ATGAGCCTCT AAAGATTTGT 
ATATCTTCAA GAAATTGCGA GGACAGAAAT ATCAGGACTT TGGGTTGTGA GGGTATTGCC 
TTGAACGATA CACCCCTGCA GGATGTGAAC AGCCAAGAGA AGGACAGTAG CCCTCCTGAT 
TTGGATTGCA GTGAGCAGGC TCTGTTGGGC AGCATTCAGA GCCAGGAAAG CCCTAATCCA 
ACCAGCTGCA GTTTGCCAGA TGTGTCCAGC AGCACCGAGA GAGTCACAGA GAGCACCGTC 
ATTACCTTAC CAGATACTGG AATGCCCACC GACTGCACCG TGGTACAGAT GAGTGAAGGC 
AGGGATGGAG AAGTCAACTT TGGGTCTTCG CAGCAAGAAA AAGAAGAGGA GGAAGCTGAG 
GATGGAGAAG AAAGAACTAT AAAAAGAAAA GAGAAATTGT TGGAGTCCCC CAAAGCCATT 
GTACCGTACA GTTCAATGCT CATCTTCAGC TCCACCAATC CCATAAGAAA AGTATGTCAT 
TACATTGTTA ACCTACGATA CTTTGAGATG AGCATCCTGC TGGTAATAGC AGCAAGTAGT 
ATAGCCTTAG CAGCGGAGGA CCCTGTACTG ACCAACTCGG ACCGCAACAA AGTTCTTCGC 
TATTTTGACT ATGTTTTCAC AGGGGTCTTC ACTTTTGAGA TGATTATAAA GATGATTGAC 
CAAGGTTTGA TTTTACACGA GGGCTCCTAC TTTCGTGACC TATGGAACAT CTTGGATTTT 
GTTGTTGTTG TGGGAGCCTT AATTGCGTTT GCCTTAGCGA CTAACAAAGG CCGGGACATC 
AAAACCATTA AGTCTCTACG TGTGTTACGG GTGCTGCGTC CACTAAAAAC AATCAAACGC 
CTGCCAAAAC TGAAGGCAGT CTTTGATTGT GTGGTTAACT CTTTAAAGAA TGTCTTTAAT 
ATTCTCATCG TATACAAACT CTTCATGTTC ATATTTGCTG TAATCGCTGT CCAGCTGTTT 
AAGGGCAAGT TCTTCTATTG TACAGACAGC TCAAAGGAGA CAGAGAAAGA GTGCATAGGG 
AGTTACATCG ATCACGAAAA GAACAAGATG GAGGTGAAGC GGAGAGAGTG GAAAAGACAT 
GATTTCCATT ATGACAACAT AATATGGGCT CTTCTTACCC TCTTTACTGT ATCAACAGGG 
GAAGGCTGGC CTCAGGTTCT GCAGCACTCT GTTGATATAA CTGAAGAAGA TAGGGGTCCA 
AGCCGAAGCA ACCGTATGGA GATGTCCATT TTCTATGTTA TCTACTTTGT GGTCTTCCCC 
TTCTTCTTTG TAAATATATT TGTGGCTCTG ATTATCATCA CATTCCAGGA GCAAGGAGAC 
AAGATGATGG AGGAATGTTC CCTAGAAAAG AATGAGAGAG CCTGCATAGA CTTTGCAATA 
AGTGCTAAGC CACTTACACG ATATATGCCA CAGAACAGGC ACACATTCCA GTACCGGGTC 
TGGCACTTCG TGGTCTCCCC ATCATTTGAA TACACTATCA TGGCTATGAT TGCACTAAAC 
ACAGTGGTAC TCATGATGAA GTATTATTCT GCGCCATACA CATATGAGCT TGCATTAAAG 
TATCTCAATA TTGCATTCAC AATGCTATTT TCTCTGGAGT GCATCCTGAA AATAATTGCA 
TTTGGGTTTT TGAATTACTT TCGGGATACT TGGAACATTT TTGACTTCAT TACTGTAATT 
GGTAGCATCA CGGAAATTAT TCTAACAGAT AGTAAGTTGG TGAACACCAG CAGCTTTAAT 
ATGAGCTTTC TGAAGCTGTT CCGAGCGGCG CGACTTATTA AACTTCTCCG GCAAGGGTAC 
ACCATCCGGA TCCTGCTGTG GACTTTTGTT CAGTCCTTTA AGGCACTTCC TTATGTGTGT 
CTTCTCATTG CAATGTTGTT CTTCATCTAT GCCATTATTG GGATGCAGGT GTTTGGCAAT 
ATCCGCCTGG AAGAGGACAG TCATATTAAT AGGCACAACA ATTTTAGAAG CTTCTTTAGT 
TCTCTTATGT TACTCTTCAG GAGTGCCACA GGAGAAGCGT GGCAGGAGAT CATGCTTTCA 
TGCCTTAGTG ATAAGGGTTG TGAGCCAGAC ACCAGTGCAG CCTCTGGACA CAATGAGAAT 
GAGCGCTGTG GAACTGAACT TGCATATGTT TATTTTGTTT CATTCATTTT CTTCTGCTCC 
TTCCTCATGC TAAATCTGTT TGTGGCTGTC ATTATGGACA ACTTTGAGTA CTTGACACGG 
GACTCTTCCA TTTTGGGACC TCATCACTTG GATGAGTTTG TTCGGATATG GGCTGAATAT 
GATCGAGCAG CATGTGGCAG GATCCCCTAT AAGGATATGT ACAAATTAGT CCGAGTCATC 
TCGCCTCCGC TCGGCCTAGG AGAGAACTGC CCATACAGGG TGGCATGCAA GAGATTGGTC 
CTGATGAATA TGCCAGTGTC GGAGGACATG ACGGTTCATT TCACATCAAC TCTTATGGCC 
CTAATACGCA CGGCACTGGA TATCAAAATA GCTAAAGGAG GTGCTGACAG GCAACAGCTG 
GACTCAGAGC TGCAGAAAGA TATACTTGCC ATTTGGCCAA ATCTCTCCCA GAAAATGATC 
GACTTACTTG TGCCAATGCC AAGAGCCTCT GATCTGACGG TGGGGAAGAT TTACGCTGCA 
ATGATGATCA TGGATTACTA TAAACAGAGC AAAGCGAAAA AACAAAGGCA GCAGCTGGAG 
GAGCAGAAAA ATGCACCTAT GTTTCAGAGA ATGGAGGCTT CTGCCCTCCC TGAGGAGATC 
TTATCCAATG CCAAAGCTTT ACCATACCTT CAGCAAGACA CTCTATCAGG CCTGGGTGGA 
TTTCCTTCAC TGAGTCCCTT GTCCCCAGAA GAAGTATTCC AGCCGCCATG CCTAGACGGG 
GAAGAGGAGG AAGACTTTGA AGAGCAGCTG TCTCTGGAGC CTGAGGTTAG AGAGTTTAGA 
AGGGAGCAGC TCCCTAACCG AGGCAGCTAC ATCCCTGTGG AGATGCTGGA ACGTCCAGCG 
TCTGTGAGAG CTTCTTCCAT GCCACGTCTG GCTGAGAATC CTCAGGTCCT GTCAGACGTA 
AGCTCCATGC GTCGCTCCTT CTCCACAACT GCAGAGAAGC GAGTGCGCAG CTCGTGGCTA 
GATGAGTTCA CTATGGACAG GAGTAGCACT GACTGTGCAT ACAGATACCA GAGGCGCAGT 
TATCATGCAT CCTTAAGACT GTCTGCACAT CGCCTGAATA CAGATACAGG TCACTGGTCA 
GATAATCACC GTTCTGGCGG AAGGGAGCGA GGAAGGTCCA AAGAGAGAAA ACACTTGCTG 
TCACCCGATC CTTCCCGCTG TAATTCTGAG GAAAGGAGCC CACAAGCTGC CTGTGAACAC 
CGAGATCACC AACTGTCCCC TGGGCATACT CCCAGTAGTG TACAGATTCT TGACTCTCTG 
AGTGAAAGTT GTACCCCTTC AGACACCAGC ACCCCAAGAA GAGGCCGTAG ACAGCTTCCT 
TCTGTGCCTC CAACTCCTAG ACCCCTTATC TCTTTCTCCT CGTTAATCCG CCGGCAGAAA 
CACAGCCCCC CTCCTCAAAA TAAAAATGAT ACTATCCTTC CTAGTGAGCC TGGTCTGACT 
GAGTCTTCCT GCTCCCTTGG GGGTCATCAG AGCTCCCAGT CCACACCACA GCATTATATC 
TCAGAGCCCT ACCTGGCACT GCATGAGGAG ATGCAAAGCT CAGACTGCCC AGCAGAGGAC 
ACACTCACCT TTGAGGTTGC TGTGGCTACT AGTCTGGGCC GCTCGAACAC CATTGGCTCT 
GCCCCTCTAC GTCATGGCTG GCAAATGCCC AATGGTCACT ACCGGCGTCG AAGGAAGGGA 
GGAATTCCAC CAGTGATACC CTCCAGAGAG TTAAGTGACA CAGAAGAGGA TGATCGATGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949087.1
CDS	9..6431
Translation	MILATIIANCIVLALEQHLPGDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGLVFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHKACYRNNSGMELDPMDPPHPCGVPRCPAGYECKEWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNSGTSALEVLRRATIKRSRTEAMNRDSTEDHCVDISSVGTPLARASIKSTKMDGASYFRHKEKLLRVSVRHMIKSQVFYWMVLGVVALNTFCVAIVHHNQTLWLSNVLYYAEFLFLGLFLVEMAFKMYGMGPRLYFHSSFNCFDCGVTIGSIFEVLWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMNSMKSIISLLFLLFLFIVVFALLGMQLYGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVRSGMWSSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNASCIEKERRKRHTLSIWEQRTSQLRRHIQMSSQEALQSEELAALNPHSSIYRKTKEAAEQNTEKEEVHQDAKLAIDPVEGPPEGEGSPSNRNLRENWRQKSRHGNSENTEPNGTTPEEAMRRRQSQRRSRHRRVRTEGREQCSNPNSTSASREGGLDKVSAEDGRTIPEEKSNGEAKEVKGVDDEPLKICISSRNCEDRNIRTLGCEGIALNDTPLQDVNSQEKDSSPPDLDCSEQALLGSIQSQESPNPTSCSLPDVSSSTERVTESTVITLPDTGMPTDCTVVQMSEGRDGEVNFGSSQQEKEEEEAEDGEERTIKRKEKLLESPKAIVPYSSMLIFSSTNPIRKVCHYIVNLRYFEMSILLVIAASSIALAAEDPVLTNSDRNKVLRYFDYVFTGVFTFEMIIKMIDQGLILHEGSYFRDLWNILDFVVVVGALIAFALATNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKETEKECIGSYIDHEKNKMEVKRREWKRHDFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDITEEDRGPSRSNRMEMSIFYVIYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPYTYELALKYLNIAFTMLFSLECILKIIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSSFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIRLEEDSHINRHNNFRSFFSSLMLLFRSATGEAWQEIMLSCLSDKGCEPDTSAASGHNENERCGTELAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRIWAEYDRAACGRIPYKDMYKLVRVISPPLGLGENCPYRVACKRLVLMNMPVSEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKDILAIWPNLSQKMIDLLVPMPRASDLTVGKIYAAMMIMDYYKQSKAKKQRQQLEEQKNAPMFQRMEASALPEEILSNAKALPYLQQDTLSGLGGFPSLSPLSPEEVFQPPCLDGEEEEDFEEQLSLEPEVREFRREQLPNRGSYIPVEMLERPASVRASSMPRLAENPQVLSDVSSMRRSFSTTAEKRVRSSWLDEFTMDRSSTDCAYRYQRRSYHASLRLSAHRLNTDTGHWSDNHRSGGRERGRSKERKHLLSPDPSRCNSEERSPQAACEHRDHQLSPGHTPSSVQILDSLSESCTPSDTSTPRRGRRQLPSVPPTPRPLISFSSLIRRQKHSPPPQNKNDTILPSEPGLTESSCSLGGHQSSQSTPQHYISEPYLALHEEMQSSDCPAEDTLTFEVAVATSLGRSNTIGSAPLRHGWQMPNGHYRRRRKGGIPPVIPSRELSDTEEDDRC

Target ORF information:

RefSeq Version	XM_018093598.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calcium channel, voltage-dependent, R type, alpha 1E subunit (cacna1e), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018093598.1

ORF Insert Sequence:

ATGATCCTTG CCACAATTAT TGCAAACTGC ATTGTCCTGG CTTTGGAGCA GCATCTTCCA 
GGCGATGACA AGACTCCAAT GTCTCGCAGA TTGGAAAAGA CTGAGCCCTA TTTCATTGGG 
ATATTTTGCT TTGAAGCTGG AATTAAAATT GTCGCTCTGG GACTGGTTTT TCACAAAGGT 
TCTTATCTTC GGAATGGCTG GAATGTCATG GACTTTATAG TAGTGCTCAG TGGCATCCTG 
GCCACAGCTG GAACTCACTT CAATACTCAC GTGGACCTGC GTACTTTGCG GGCAGTGCGT 
GTTCTCAGGC CGTTGAAGCT GGTCTCGGGG ATTCCCAGTT TACAGATTGT GTTGAAGTCA 
ATCATGAAGG CGATGGTCCC TCTGCTACAG ATTGGATTGT TGCTCTTCTT TGCCATCCTT 
ATGTTTGCTA TCATCGGCCT TGAGTTTTAC AGTGGAAAGC TACACAAGGC CTGTTACAGG 
AACAACTCTG GAATGGAGCT AGACCCCATG GATCCACCTC ACCCTTGTGG AGTACCCCGC 
TGCCCAGCTG GGTATGAGTG CAAGGAATGG ATCGGACCCA ATGATGGAAT AACACAGTTT 
GACAACATCT TATTTGCGGT GCTGACAGTC TTCCAGTGTA TAACTATGGA GGGCTGGACC 
ACAGTGCTGT ACAATACCAA TGATGCCTTA GGAGCCACAT GGAACTGGCT GTACTTCATT 
CCCCTCATTA TCATCGGATC CTTCTTTGTC CTTAATCTTG TCCTTGGAGT GCTGTCCGGT 
GAATTTGCCA AAGAGAGGGA AAGAGTGGAA AACCGCCGGG CTTTCATGAA GTTGCGCAGG 
CAGCAGCAGA TTGAGAGGGA GTTGAATGGC TATCGGGCAT GGATAGATAA AGCTGAGGAA 
GTCATGTTGG CAGAAGAAAA CAAAAATTCT GGGACCTCTG CTTTAGAAGT TCTTAGGCGA 
GCAACCATTA AACGAAGTAG AACAGAAGCC ATGAACCGGG ACTCTACTGA GGACCATTGT 
GTTGACATAT CCTCTGTTGG CACCCCCCTT GCTAGAGCAA GCATCAAAAG TACCAAAATG 
GATGGGGCAT CATATTTTCG GCACAAGGAA AAACTATTGC GTGTCTCCGT TCGTCACATG 
ATCAAATCCC AGGTTTTCTA CTGGATGGTC CTGGGAGTGG TAGCACTTAA CACTTTCTGT 
GTGGCTATTG TCCATCACAA CCAGACCCTG TGGCTCTCAA ATGTTCTTTA CTATGCAGAA 
TTTCTGTTTC TTGGGTTATT TTTGGTGGAG ATGGCTTTTA AAATGTATGG AATGGGTCCA 
CGTCTTTACT TTCACTCATC CTTCAACTGC TTTGATTGCG GGGTCACAAT AGGTAGTATA 
TTTGAAGTAC TTTGGGCCAT ATTTAGACCA GGAACTTCCT TTGGAATCAG TGTCTTAAGA 
GCTCTCCGAC TTTTGAGAAT CTTCAAAATA ACAAAATACT GGGCCTCACT ACGAAACCTT 
GTAGTATCAT TGATGAATTC TATGAAATCC ATCATCAGCC TGCTCTTCCT TCTATTCCTC 
TTCATAGTTG TTTTTGCTCT CCTTGGAATG CAGTTATATG GTGGCAGATT CAATTTTAAT 
GATGGCACTC CTTCTGCAAA CTTTGATACA TTCCCTGCAG CCATCATGAC TGTATTCCAG 
ATTCTGACAG GAGAGGATTG GAATGAAGTG ATGTACAATG GTATTCGCTC CCAAGGTGGT 
GTTCGATCCG GAATGTGGTC TTCCATATAC TTTATTGTCC TTACCCTTTT TGGAAATTAC 
ACACTGCTAA ATGTATTTCT TGCCATTGCT GTGGACAACC TGGCTAATGC ACAGGAGTTA 
ACTAAGGATG AGCAAGAAGA AGAGGAAGCT TTCAATCAGA AGCATGCACT GCAAAAAGCG 
AAGGAGGTCA GCCCCATGTC TGCACCTAAT GCATCATGTA TTGAGAAGGA GCGCAGAAAA 
CGGCACACAT TGTCTATATG GGAGCAGCGC ACAAGCCAGC TAAGGAGGCA CATACAGATG 
TCTAGTCAAG AAGCTCTCCA GAGTGAAGAA CTGGCAGCAT TGAATCCACA CTCCAGTATC 
TATCGCAAGA CAAAGGAAGC TGCTGAACAA AACACAGAAA AAGAAGAAGT GCATCAAGAC 
GCAAAACTGG CAATAGACCC AGTTGAAGGA CCACCAGAAG GTGAAGGAAG CCCTTCTAAT 
AGAAATCTCA GGGAAAACTG GCGCCAGAAG TCCAGGCATG GAAATTCTGA AAACACTGAA 
CCAAATGGAA CAACTCCAGA AGAAGCAATG CGCCGCAGAC AAAGTCAGAG ACGCAGCCGT 
CATAGAAGAG TTAGAACTGA AGGTCGTGAG CAGTGTAGCA ATCCAAACAG TACCTCAGCA 
AGCAGAGAAG GTGGTCTTGA CAAAGTCTCT GCAGAGGATG GAAGAACAAT TCCAGAAGAG 
AAGAGCAATG GAGAAGCCAA AGAAGTCAAA GGGGTTGATG ATGAGCCTCT AAAGATTTGT 
ATATCTTCAA GAAATTGCGA GGACAGAAAT ATCAGGACTT TGGGTTGTGA GGGTATTGCC 
TTGAACGATA CACCCCTGCA GGATGTGAAC AGCCAAGAGA AGGACAGTAG CCCTCCTGAT 
TTGGATTGCA GTGAGCAGGC TCTGTTGGGC AGCATTCAGA GCCAGGAAAG CCCTAATCCA 
ACCAGCTGCA GTTTGCCAGA TGTGTCCAGC AGCACCGAGA GAGTCACAGA GAGCACCGTC 
ATTACCTTAC CAGATACTGG AATGCCCACC GACTGCACCG TGGTACAGAT GAGTGAAGGC 
AGGGATGGAG AAGTCAACTT TGGGTCTTCG CAGCAAGAAA AAGAAGAGGA GGAAGCTGAG 
GATGGAGAAG AAAGAACTAT AAAAAGAAAA GAGAAATTGT TGGAGTCCCC CAAAGCCATT 
GTACCGTACA GTTCAATGCT CATCTTCAGC TCCACCAATC CCATAAGAAA AGTATGTCAT 
TACATTGTTA ACCTACGATA CTTTGAGATG AGCATCCTGC TGGTAATAGC AGCAAGTAGT 
ATAGCCTTAG CAGCGGAGGA CCCTGTACTG ACCAACTCGG ACCGCAACAA AGTTCTTCGC 
TATTTTGACT ATGTTTTCAC AGGGGTCTTC ACTTTTGAGA TGATTATAAA GATGATTGAC 
CAAGGTTTGA TTTTACACGA GGGCTCCTAC TTTCGTGACC TATGGAACAT CTTGGATTTT 
GTTGTTGTTG TGGGAGCCTT AATTGCGTTT GCCTTAGCGA CTAACAAAGG CCGGGACATC 
AAAACCATTA AGTCTCTACG TGTGTTACGG GTGCTGCGTC CACTAAAAAC AATCAAACGC 
CTGCCAAAAC TGAAGGCAGT CTTTGATTGT GTGGTTAACT CTTTAAAGAA TGTCTTTAAT 
ATTCTCATCG TATACAAACT CTTCATGTTC ATATTTGCTG TAATCGCTGT CCAGCTGTTT 
AAGGGCAAGT TCTTCTATTG TACAGACAGC TCAAAGGAGA CAGAGAAAGA GTGCATAGGG 
AGTTACATCG ATCACGAAAA GAACAAGATG GAGGTGAAGC GGAGAGAGTG GAAAAGACAT 
GATTTCCATT ATGACAACAT AATATGGGCT CTTCTTACCC TCTTTACTGT ATCAACAGGG 
GAAGGCTGGC CTCAGGTTCT GCAGCACTCT GTTGATATAA CTGAAGAAGA TAGGGGTCCA 
AGCCGAAGCA ACCGTATGGA GATGTCCATT TTCTATGTTA TCTACTTTGT GGTCTTCCCC 
TTCTTCTTTG TAAATATATT TGTGGCTCTG ATTATCATCA CATTCCAGGA GCAAGGAGAC 
AAGATGATGG AGGAATGTTC CCTAGAAAAG AATGAGAGAG CCTGCATAGA CTTTGCAATA 
AGTGCTAAGC CACTTACACG ATATATGCCA CAGAACAGGC ACACATTCCA GTACCGGGTC 
TGGCACTTCG TGGTCTCCCC ATCATTTGAA TACACTATCA TGGCTATGAT TGCACTAAAC 
ACAGTGGTAC TCATGATGAA GTATTATTCT GCGCCATACA CATATGAGCT TGCATTAAAG 
TATCTCAATA TTGCATTCAC AATGCTATTT TCTCTGGAGT GCATCCTGAA AATAATTGCA 
TTTGGGTTTT TGAATTACTT TCGGGATACT TGGAACATTT TTGACTTCAT TACTGTAATT 
GGTAGCATCA CGGAAATTAT TCTAACAGAT AGTAAGTTGG TGAACACCAG CAGCTTTAAT 
ATGAGCTTTC TGAAGCTGTT CCGAGCGGCG CGACTTATTA AACTTCTCCG GCAAGGGTAC 
ACCATCCGGA TCCTGCTGTG GACTTTTGTT CAGTCCTTTA AGGCACTTCC TTATGTGTGT 
CTTCTCATTG CAATGTTGTT CTTCATCTAT GCCATTATTG GGATGCAGGT GTTTGGCAAT 
ATCCGCCTGG AAGAGGACAG TCATATTAAT AGGCACAACA ATTTTAGAAG CTTCTTTAGT 
TCTCTTATGT TACTCTTCAG GAGTGCCACA GGAGAAGCGT GGCAGGAGAT CATGCTTTCA 
TGCCTTAGTG ATAAGGGTTG TGAGCCAGAC ACCAGTGCAG CCTCTGGACA CAATGAGAAT 
GAGCGCTGTG GAACTGAACT TGCATATGTT TATTTTGTTT CATTCATTTT CTTCTGCTCC 
TTCCTCATGC TAAATCTGTT TGTGGCTGTC ATTATGGACA ACTTTGAGTA CTTGACACGG 
GACTCTTCCA TTTTGGGACC TCATCACTTG GATGAGTTTG TTCGGATATG GGCTGAATAT 
GATCGAGCAG CATGTGGCAG GATCCCCTAT AAGGATATGT ACAAATTAGT CCGAGTCATC 
TCGCCTCCGC TCGGCCTAGG AGAGAACTGC CCATACAGGG TGGCATGCAA GAGATTGGTC 
CTGATGAATA TGCCAGTGTC GGAGGACATG ACGGTTCATT TCACATCAAC TCTTATGGCC 
CTAATACGCA CGGCACTGGA TATCAAAATA GCTAAAGGAG GTGCTGACAG GCAACAGCTG 
GACTCAGAGC TGCAGAAAGA TATACTTGCC ATTTGGCCAA ATCTCTCCCA GAAAATGATC 
GACTTACTTG TGCCAATGCC AAGAGCCTCT GATCTGACGG TGGGGAAGAT TTACGCTGCA 
ATGATGATCA TGGATTACTA TAAACAGAGC AAAGCGAAAA AACAAAGGCA GCAGCTGGAG 
GAGCAGAAAA ATGCACCTAT GTTTCAGAGA ATGGAGGCTT CTGCCCTCCC TGAGGAGATC 
TTATCCAATG CCAAAGCTTT ACCATACCTT CAGCAAGACA CTCTATCAGG CCTGGGTGGA 
TTTCCTTCAC TGAGTCCCTT GTCCCCAGAA GAAGTATTCC AGCCGCCATG CCTAGACGGG 
GAAGAGGAGG AAGACTTTGA AGAGCAGCTG TCTCTGGAGC CTGAGGTTAG AGAGTTTAGA 
AGGGAGCAGC TCCCTAACCG AGGCAGCTAC ATCCCTGTGG AGATGCTGGA ACGTCCAGCG 
TCTGTGAGAG CTTCTTCCAT GCCACGTCTG GCTGAGAATC CTCAGGTCCT GTCAGACGTA 
AGCTCCATGC GTCGCTCCTT CTCCACAACT GCAGAGAAGC GAGTGCGCAG CTCGTGGCTA 
GATGAGTTCA CTATGGACAG GAGTAGCACT GACTGTGCAT ACAGATACCA GAGGCGCAGT 
TATCATGCAT CCTTAAGACT GTCTGCACAT CGCCTGAATA CAGATACAGG TCACTGGTCA 
GATAATCACC GTTCTGGCGG AAGGGAGCGA GGAAGGTCCA AAGAGAGAAA ACACTTGCTG 
TCACCCGATC CTTCCCGCTG TAATTCTGAG GAAAGGAGCC CACAAGCTGC CTGTGAACAC 
CGAGATCACC AACTGTCCCC TGGGCATACT CCCAGTAGTG TACAGATTCT TGACTCTCTG 
AGTGAAAGTT GTACCCCTTC AGACACCAGC ACCCCAAGAA GAGGCCGTAG ACAGCTTCCT 
TCTGTGCCTC CAACTCCTAG ACCCCTTATC TCTTTCTCCT CGTTAATCCG CCGGCAGAAA 
CACAGCCCCC CTCCTCAAAA TAAAAATGAT ACTATCCTTC CTAGTGAGCC TGGTCTGACT 
GAGTCTTCCT GCTCCCTTGG GGGTCATCAG AGCTCCCAGT CCACACCACA GCATTATATC 
TCAGAGCCCT ACCTGGCACT GCATGAGGAG ATGCAAAGCT CAGACTGCCC AGCAGAGGAC 
ACACTCACCT TTGAGGTTGC TGTGGCTACT AGTCTGGGCC GCTCGAACAC CATTGGCTCT 
GCCCCTCTAC GTCATGGCTG GCAAATGCCC AATGGTCACT ACCGGCGTCG AAGGAAGGGA 
GGAATTCCAC CAGTGATACC CTCCAGAGAG TTAAGTGACA CAGAAGAGGA TGATCGATGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1e ( XM_031900979.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756839.1 Xenopus tropicalis calcium channel, voltage-dependent, R type, alpha 1E subunit (cacna1e), transcript variant X1, mRNA.

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CloneID	OXa57229
Clone ID Related Accession (Same CDS sequence)	XM_031900979.1
Accession Version	XM_031900979.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7182bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTATTAA AGATGCTGGG ATTAAGCTCC TGTTCAACCA GTGCTCATAC ACTCAATGAG 
CCTGACCAGT CAAGCACACT AGCCATACCA GCATTGGATG CCGACACTCA GAGATATGTC 
TTGGCCCTTC CAGAAGCAGA GACATGGCGC AAGCATCGAT TGGGCCTGAC ACAGGCTACT 
CACTCTTGCA ATCTTCTAGA ACCTGAGACA CTTTCTGAAA CCCTAGTTTC ACGCACTGCA 
AGTTACGATG CCTTGTATGA CCTGCGAGCA AGAGAAGCGG AGTCTAACTT CCCATTTGAA 
GGCAGCAGCA TGTTTGAGGT GACGTCTGGG ATGGATTCTG AGTTTCCTTT AAGCCCCCAT 
GTTATAAAGC GAAGGAGAGG AGGCCTGATA GAACAAAAGG ATATCATCAA GGCCCACGAA 
GCTCATAAGA TTCAGAGTAC TCCCCAGGCT CGACGGAAGG AATGGGAGAT GGCTCGTTTC 
GGAGAGGCTG TGACTGGCAG GTTGAGCTCC GCAGATGGGG GCTCAGAGAG GAGCAAGACC 
CTACAAGGTT CCCCAGCTGC ACCTGTTGGG ACCCAAGCAG CTTTCAAGCA ATCCAAAGCC 
CAGAGGGCAA GGACCATGGC TTTATACAAC CCCATCCCTG TCCGACAGAA CTGCTTCACC 
GTGAACAGGT CTCTCTTCAT TTTCGGAGAA GATAATATTA TCAGGAAATA CGCCAAGAAG 
CTCATTGATT GGCCTCCTTT TGAATACATG ATCCTTGCCA CAATTATTGC AAACTGCATT 
GTCCTGGCTT TGGAGCAGCA TCTTCCAGGC GATGACAAGA CTCCAATGTC TCGCAGATTG 
GAAAAGACTG AGCCCTATTT CATTGGGATA TTTTGCTTTG AAGCTGGAAT TAAAATTGTC 
GCTCTGGGAC TGGTTTTTCA CAAAGGTTCT TATCTTCGGA ATGGCTGGAA TGTCATGGAC 
TTTATAGTAG TGCTCAGTGG CATCCTGGCC ACAGCTGGAA CTCACTTCAA TACTCACGTG 
GACCTGCGTA CTTTGCGGGC AGTGCGTGTT CTCAGGCCGT TGAAGCTGGT CTCGGGGATT 
CCCAGTTTAC AGATTGTGTT GAAGTCAATC ATGAAGGCGA TGGTCCCTCT GCTACAGATT 
GGATTGTTGC TCTTCTTTGC CATCCTTATG TTTGCTATCA TCGGCCTTGA GTTTTACAGT 
GGAAAGCTAC ACAAGGCCTG TTACAGGAAC AACTCTGGAA TGGAGCTAGA CCCCATGGAT 
CCACCTCACC CTTGTGGAGT GCCCCGCTGC CCAGCTGGGT ATGAGTGCAA GGAATGGATC 
GGACCCAATG ATGGAATAAC ACAGTTTGAC AACATCCTAT TTGCGGTGCT GACAGTCTTC 
CAGTGTATAA CTATGGAGGG CTGGACCACA GTGCTGTACA ATACCAATGA TGCCTTAGGA 
GCCACATGGA ACTGGCTGTA CTTCATTCCC CTCATTATCA TCGGATCCTT CTTTGTCCTT 
AATCTTGTCC TTGGAGTGCT GTCCGGTGAA TTTGCCAAAG AGAGGGAAAG AGTGGAAAAC 
CGCCGGGCTT TCATGAAGTT GCGCAGGCAG CAGCAGATTG AGAGGGAGTT GAATGGCTAT 
CGGGCATGGA TAGATAAAGC TGAGGAAGTC ATGTTGGCAG AAGAAAACAA AAATTCTGGG 
ACCTCTGCTT TAGAAGTTCT TAGGCGAGCA ACCATTAAAC GAAGTAGAAC AGAAGCCATG 
AACCGGGACT CTACTGAGGA CCATTGTGTT GACATATCCT CTGTTGGCAC CCCCCTTGCT 
AGAGCAAGCA TCAAAAGTAC CAAAATGGAT GGGGCATCAT ATTTTCGGCA CAAGGAAAAA 
CTATTGCGTG TCTCCGTTCG TCACATGATC AAATCCCAGG TTTTCTACTG GATGGTCCTG 
GGAGTGGTAG CACTTAACAC TTTCTGTGTG GCTATTGTCC ATCACAACCA GACCCTGTGG 
CTCTCAAATG TTCTTTACTA TGCAGAATTT CTGTTTCTTG GGTTATTTTT GGTGGAGATG 
GCTTTTAAAA TGTATGGAAT GGGTCCACGT CTTTACTTTC ACTCATCCTT CAACTGCTTT 
GATTGCGGGG TCACAATAGG TAGTATATTT GAAGTACTTT GGGCCATATT TAGACCAGGA 
ACTTCCTTTG GAATCAGTGT CTTAAGAGCT CTCCGACTTT TGAGAATCTT CAAAATAACA 
AAATACTGGG CCTCACTACG AAACCTTGTA GTATCATTGA TGAATTCTAT GAAATCCATC 
ATCAGCCTGC TCTTCCTTCT ATTCCTCTTC ATAGTTGTTT TTGCTCTCCT TGGAATGCAG 
TTATATGGTG GCAGATTCAA TTTTAATGAT GGCACTCCTT CTGCAAACTT TGATACATTC 
CCTGCAGCCA TCATGACTGT ATTCCAGATT CTGACAGGAG AGGATTGGAA TGAAGTGATG 
TACAATGGTA TTCGCTCCCA AGGTGGTGTT CGATCCGGAA TGTGGTCTTC CATATACTTT 
ATTGTCCTTA CCCTTTTTGG AAATTACACA CTGCTAAATG TATTTCTTGC CATTGCTGTG 
GACAACCTGG CTAATGCACA GGAGTTAACT AAGGATGAGC AAGAAGAAGA GGAAGCTTTC 
AATCAGAAGC ATGCACTGCA AAAAGCGAAG GAGGTCAGCC CCATGTCTGC ACCTAATGCA 
TCATGTATTG AGAAGGAGCG CAGAAAACGG CACACATTGT CTATATGGGA GCAGCGCACA 
AGCCAGCTAA GGAGGCACAT ACAGATGTCT AGTCAAGAAG CTCTCCAGAG TGAAGAACTG 
GCAGCATTGA ATCCACACTC CAGTATCTAT CGCAAGACAA AGGAAGCTGC TGAACAAAAC 
ACAGAAAAAG AAGAAGTGCA TCAAGACGCA AAACTGGCAA TAGACCCAGT TGAAGGACCA 
CCAGAAGGTG AAGGAAGCCC TTCTAATAGA AATCTCAGGG AAAACTGGCG CCAGAAGTCC 
AGGCATGGAA ATTCTGAAAA CACTGAGCCA AATGGAACAA CTCCAGAAGA AGCAATGCGC 
CGCAGACAAA GTCAGAGACG CAGCCGTCAT AGAAGAGTTA GAACTGAAGG TCGTGAGCAG 
TGTAGCAATC CAAACAGTAC CTCAGCAAGC AGAGAAGGTG GTCTTGACAA AGTCTCTGCA 
GAGGATGGAA GAACAATTCC AGAAGAGAAG AGCAATGGAG AAGCCAAAGA AGTCAAAGGG 
GTTGATGATG AGCCTCTAAA GATTTGTATA TCTTCAAGAA ATTGCGAGGA CAGAAATATC 
AGGACTTTGG GTTGTGAGGG TATTGCCTTG AACGATACAC CCCTGCAGGA TGTGAACAGC 
CAAGAGAAGG ACAGTAGCCC TCCTGATTTG GATTGCAGTG AGCAGGCTCT GTTGGGCAGC 
ATTCAGAGCC AGGAAAGCCC TAATCCAACC AGCTGCAGTT TGCCAGATGT GTCCAGCAGC 
ACCGAGAGAG TCACAGAGAG CACCGTCATT ACCTTACCAG ATACTGGAAT GCCCACCGAC 
TGCACCGTGG TACAGATGAG TGAAGGCAGG GATGGAGAAG TCAACTTTGG GTCTTCGCAG 
CAAGAAAAAG AAGAGGAGGA AGCTGAGGAT GGAGAAGAAA GAACTATAAA AAGAAAAGAG 
AAATTGTTGG AGTCCCCCAA AGCCATTGTA CCGTACAGTT CAATGCTCAT CTTCAGCTCC 
ACCAATCCCA TAAGAAAAGT ATGTCATTAC ATTGTTAACC TACGATACTT TGAGATGAGC 
ATCCTGCTGG TAATAGCAGC AAGTAGTATA GCCTTAGCAG CGGAGGACCC TGTACTGACC 
AACTCGGACC GCAACAAAGT TCTTCGCTAT TTTGACTATG TTTTCACAGG GGTCTTCACT 
TTTGAGATGA TTATAAAGAT GATTGACCAA GGTTTGATTT TACACGAGGG CTCCTACTTT 
CGTGACCTAT GGAACATCTT GGATTTTGTT GTTGTTGTGG GAGCCTTAAT TGCGTTTGCC 
TTAGCGAATG TTTTGGGGAC TAACAAAGGC CGGGACATCA AAACCATTAA GTCTCTACGT 
GTGTTACGGG TGCTGCGTCC ACTAAAAACA ATCAAACGCC TGCCAAAACT GAAGGCAGTC 
TTTGATTGTG TGGTTAACTC TTTAAAGAAT GTCTTTAATA TTCTCATCGT ATACAAACTC 
TTCATGTTCA TATTTGCTGT AATCGCTGTC CAGCTGTTTA AGGGCAAGTT CTTCTATTGT 
ACAGACAGCT CAAAGGAGAC AGAGAAAGAG TGCATAGGGA GTTACATCGA TCACGAAAAG 
AACAAGATGG AGGTGAAGCG GAGAGAGTGG AAAAGACATG ATTTCCATTA TGACAACATA 
ATATGGGCTC TTCTTACCCT CTTTACTGTA TCAACAGGGG AAGGCTGGCC TCAGGTTCTG 
CAGCACTCTG TTGATATAAC TGAAGAAGAT AGGGGTCCAA GCCGAAGCAA CCGTATGGAG 
ATGTCCATTT TCTATGTTAT CTACTTTGTG GTCTTCCCCT TCTTCTTTGT AAATATATTT 
GTGGCTCTGA TTATCATCAC ATTCCAGGAG CAAGGAGACA AGATGATGGA GGAATGTTCC 
CTAGAAAAGA ATGAGAGAGC CTGCATAGAC TTTGCAATAA GTGCTAAGCC ACTTACACGA 
TATATGCCAC AGAACAGGCA CACATTCCAG TACCGGGTCT GGCACTTCGT GGTCTCCCCA 
TCATTTGAAT ACACTATCAT GGCTATGATT GCACTAAACA CAGTGGTACT CATGATGAAG 
TATTATTCTG CGCCATACAC ATATGAGCTT GCATTAAAGT ATCTCAATAT TGCATTCACA 
ATGCTATTTT CTCTGGAGTG CATCCTGAAA ATAATTGCAT TTGGGTTTTT GAATTACTTT 
CGGGATACTT GGAACATTTT TGACTTCATT ACTGTAATTG GTAGCATCAC GGAAATTATT 
CTAACAGATA GTAAGTTGGT GAACACCAGC AGCTTTAATA TGAGCTTTCT GAAGCTGTTC 
CGAGCGGCGC GACTTATTAA ACTTCTCCGG CAAGGGTACA CCATCCGGAT CCTGCTGTGG 
ACTTTTGTTC AGTCCTTTAA GGCACTTCCT TATGTGTGTC TTCTCATTGC AATGTTGTTC 
TTCATCTATG CCATTATTGG GATGCAGGTG TTTGGCAATA TCCGCCTGGA AGAGGACAGT 
CATATTAATA GGCACAACAA TTTTAGAAGC TTCTTTAGTT CTCTTATGTT ACTCTTCAGG 
AGTGCCACAG GAGAAGCGTG GCAGGAGATC ATGCTTTCAT GCCTTAGTGA TAAGGGTTGT 
GAGCCAGACA CCAGTGCAGC CTCTGGACAC AACGAGAATG AGCGCTGTGG AACTGAACTT 
GCATATGTTT ATTTTGTTTC ATTCATTTTC TTCTGCTCCT TCCTCATGCT AAATCTGTTT 
GTGGCTGTCA TTATGGACAA CTTTGAGTAC TTGACACGGG ACTCTTCCAT TTTGGGACCT 
CATCACTTGG ATGAGTTTGT TCGGATATGG GCTGAATATG ATCGAGCAGC ATGTGGCCGT 
ATCCATTACA CAGAGATGTA TGACATGCTG ACTCTCATGT CACCACCTCT AGGCCTTGGA 
AAGAGATGCC CTAACAAAGT GGCTTATAAG AGATTGGTCC TGATGAATAT GCCAGTGTCG 
GAGGACATGA CGGTTCATTT CACATCAACT CTTATGGCCC TAATACGCAC GGCACTGGAT 
ATCAAAATAG CTAAAGGAGG TGCTGACAGG CAACAGCTGG ACTCAGAGCT GCAGAAAGAT 
ATACTTGCCA TTTGGCCAAA TCTCTCCCAG AAAATGATCG ACTTACTTGT GCCAATGCCA 
AGAGCCTCTG ATCTGACGGT GGGGAAGATT TACGCTGCAA TGATGATCAT GGATTACTAT 
AAACAGAGCA AAGCGAAAAA ACAAAGGCAG CAGCTGGAGG AGCAGAAAAA TGCACCTATG 
TTTCAGAGAA TGGAGGCTTC TGCCCTCCCT GAGGAGATCT TATCCAATGC CAAAGCTTTA 
CCATACCTTC AGCAAGACAC TCTATCAGGC CTGGGTGGAT TTCCTTCACT GAGTCCCTTG 
TCCCCAGAAG AAGTATTCCA GCCGCCATGC CTAGACGGGG AAGAGGAGGA AGACTTTGAA 
GAGCAGCTGT CTCTGGAGCC TGAGGTTAGA GAGTTTAGAA GGGAGCAGCT CCCTAACCGA 
GGCAGCTACA TCCCTGTGGA GATGCTGGAA CGTCCAGCGT CTGTGAGAGC TTCTTCCATG 
CCACGTCTGG CTGAGAATCC TCAGGTCCTG TCAGACGTAA GCTCCATGCG TCGCTCCTTC 
TCCACAACTG CAGAGAAGCG AGTGCGCAGC TCGTGGCTAG ATGAGTTCAC TATGGACAGG 
AGTAGCACTG ACTGTGCATA CAGATACCAG AGGCGCAGTT ATCATGCATC CTTAAGACTG 
TCTGCACATC GCCTGAATAC AGATACAGGT CACTGGTCAG ATAATCACCG TTCTGGCGGA 
AGGGAGCGAG GAAGGTCCAA AGAGAGAAAA CACTTGCTGT CACCCGATCC TTCCCGCTGT 
AATTCTGAGG AAAGGAGCCC ACAAGCTGCC TGTGAACACC GAGATCACCA ACTGTCCCCT 
GGGCATACTC CCAGTAGTGT ACAGATTCTT GACTCTCTGA GTGAAAGTTG TACCCCTTCA 
GACACCAGCA CCCCAAGAAG AGGCCGTAGA CAGCTTCCTT CTGTGCCTCC AACTCCTAGA 
CCCCTTATCT CTTTCTCCTC GTTAATCCGC CGGCAGAAAC ACAGCCCCCC TCCTCAAAAT 
AAAAATGATA CTATCCTTCC TAGTGAGCCT GGTCTGACTG AGTCTTCCTG CTCCCTTGGG 
GGTCATCAGA GCTCCCAGTC CACACCACAG CATTATATCT CAGAGCCCTA CCTGGCACTG 
CATGAGGAGA TGCAAAGCTC AGACTGCCCA GCAGAGGACA CACTCACCTT TGAGGTTGCT 
GTGGCTACTA GTCTGGGCCG CTCGAACACC ATTGGCTCTG CCCCTCTACG TCATGGCTGG 
CAAATGCCCA ATGGTCACTA CCGGCGTCGA AGGAAGGGAG GAATTCCACC AGTGATACCC 
TCCAGAGAGT TAAGTGACAC AGAAGAGGAT GATCGATGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756839.1
CDS	284..7465
Translation	MVLKMLGLSSCSTSAHTLNEPDQSSTLAIPALDADTQRYVLALPEAETWRKHRLGLTQATHSCNLLEPETLSETLVSRTASYDALYDLRAREAESNFPFEGSSMFEVTSGMDSEFPLSPHVIKRRRGGLIEQKDIIKAHEAHKIQSTPQARRKEWEMARFGEAVTGRLSSADGGSERSKTLQGSPAAPVGTQAAFKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIIRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPGDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGLVFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHKACYRNNSGMELDPMDPPHPCGVPRCPAGYECKEWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNSGTSALEVLRRATIKRSRTEAMNRDSTEDHCVDISSVGTPLARASIKSTKMDGASYFRHKEKLLRVSVRHMIKSQVFYWMVLGVVALNTFCVAIVHHNQTLWLSNVLYYAEFLFLGLFLVEMAFKMYGMGPRLYFHSSFNCFDCGVTIGSIFEVLWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMNSMKSIISLLFLLFLFIVVFALLGMQLYGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVRSGMWSSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNASCIEKERRKRHTLSIWEQRTSQLRRHIQMSSQEALQSEELAALNPHSSIYRKTKEAAEQNTEKEEVHQDAKLAIDPVEGPPEGEGSPSNRNLRENWRQKSRHGNSENTEPNGTTPEEAMRRRQSQRRSRHRRVRTEGREQCSNPNSTSASREGGLDKVSAEDGRTIPEEKSNGEAKEVKGVDDEPLKICISSRNCEDRNIRTLGCEGIALNDTPLQDVNSQEKDSSPPDLDCSEQALLGSIQSQESPNPTSCSLPDVSSSTERVTESTVITLPDTGMPTDCTVVQMSEGRDGEVNFGSSQQEKEEEEAEDGEERTIKRKEKLLESPKAIVPYSSMLIFSSTNPIRKVCHYIVNLRYFEMSILLVIAASSIALAAEDPVLTNSDRNKVLRYFDYVFTGVFTFEMIIKMIDQGLILHEGSYFRDLWNILDFVVVVGALIAFALANVLGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKETEKECIGSYIDHEKNKMEVKRREWKRHDFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDITEEDRGPSRSNRMEMSIFYVIYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPYTYELALKYLNIAFTMLFSLECILKIIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSSFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIRLEEDSHINRHNNFRSFFSSLMLLFRSATGEAWQEIMLSCLSDKGCEPDTSAASGHNENERCGTELAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRIWAEYDRAACGRIHYTEMYDMLTLMSPPLGLGKRCPNKVAYKRLVLMNMPVSEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKDILAIWPNLSQKMIDLLVPMPRASDLTVGKIYAAMMIMDYYKQSKAKKQRQQLEEQKNAPMFQRMEASALPEEILSNAKALPYLQQDTLSGLGGFPSLSPLSPEEVFQPPCLDGEEEEDFEEQLSLEPEVREFRREQLPNRGSYIPVEMLERPASVRASSMPRLAENPQVLSDVSSMRRSFSTTAEKRVRSSWLDEFTMDRSSTDCAYRYQRRSYHASLRLSAHRLNTDTGHWSDNHRSGGRERGRSKERKHLLSPDPSRCNSEERSPQAACEHRDHQLSPGHTPSSVQILDSLSESCTPSDTSTPRRGRRQLPSVPPTPRPLISFSSLIRRQKHSPPPQNKNDTILPSEPGLTESSCSLGGHQSSQSTPQHYISEPYLALHEEMQSSDCPAEDTLTFEVAVATSLGRSNTIGSAPLRHGWQMPNGHYRRRRKGGIPPVIPSRELSDTEEDDRC

Target ORF information:

RefSeq Version	XM_031900979.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calcium channel, voltage-dependent, R type, alpha 1E subunit (cacna1e), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031900979.1

ORF Insert Sequence:

ATGGTATTAA AGATGCTGGG ATTAAGCTCC TGTTCAACCA GTGCTCATAC ACTCAATGAG 
CCTGACCAGT CAAGCACACT AGCCATACCA GCATTGGATG CCGACACTCA GAGATATGTC 
TTGGCCCTTC CAGAAGCAGA GACATGGCGC AAGCATCGAT TGGGCCTGAC ACAGGCTACT 
CACTCTTGCA ATCTTCTAGA ACCTGAGACA CTTTCTGAAA CCCTAGTTTC ACGCACTGCA 
AGTTACGATG CCTTGTATGA CCTGCGAGCA AGAGAAGCGG AGTCTAACTT CCCATTTGAA 
GGCAGCAGCA TGTTTGAGGT GACGTCTGGG ATGGATTCTG AGTTTCCTTT AAGCCCCCAT 
GTTATAAAGC GAAGGAGAGG AGGCCTGATA GAACAAAAGG ATATCATCAA GGCCCACGAA 
GCTCATAAGA TTCAGAGTAC TCCCCAGGCT CGACGGAAGG AATGGGAGAT GGCTCGTTTC 
GGAGAGGCTG TGACTGGCAG GTTGAGCTCC GCAGATGGGG GCTCAGAGAG GAGCAAGACC 
CTACAAGGTT CCCCAGCTGC ACCTGTTGGG ACCCAAGCAG CTTTCAAGCA ATCCAAAGCC 
CAGAGGGCAA GGACCATGGC TTTATACAAC CCCATCCCTG TCCGACAGAA CTGCTTCACC 
GTGAACAGGT CTCTCTTCAT TTTCGGAGAA GATAATATTA TCAGGAAATA CGCCAAGAAG 
CTCATTGATT GGCCTCCTTT TGAATACATG ATCCTTGCCA CAATTATTGC AAACTGCATT 
GTCCTGGCTT TGGAGCAGCA TCTTCCAGGC GATGACAAGA CTCCAATGTC TCGCAGATTG 
GAAAAGACTG AGCCCTATTT CATTGGGATA TTTTGCTTTG AAGCTGGAAT TAAAATTGTC 
GCTCTGGGAC TGGTTTTTCA CAAAGGTTCT TATCTTCGGA ATGGCTGGAA TGTCATGGAC 
TTTATAGTAG TGCTCAGTGG CATCCTGGCC ACAGCTGGAA CTCACTTCAA TACTCACGTG 
GACCTGCGTA CTTTGCGGGC AGTGCGTGTT CTCAGGCCGT TGAAGCTGGT CTCGGGGATT 
CCCAGTTTAC AGATTGTGTT GAAGTCAATC ATGAAGGCGA TGGTCCCTCT GCTACAGATT 
GGATTGTTGC TCTTCTTTGC CATCCTTATG TTTGCTATCA TCGGCCTTGA GTTTTACAGT 
GGAAAGCTAC ACAAGGCCTG TTACAGGAAC AACTCTGGAA TGGAGCTAGA CCCCATGGAT 
CCACCTCACC CTTGTGGAGT GCCCCGCTGC CCAGCTGGGT ATGAGTGCAA GGAATGGATC 
GGACCCAATG ATGGAATAAC ACAGTTTGAC AACATCCTAT TTGCGGTGCT GACAGTCTTC 
CAGTGTATAA CTATGGAGGG CTGGACCACA GTGCTGTACA ATACCAATGA TGCCTTAGGA 
GCCACATGGA ACTGGCTGTA CTTCATTCCC CTCATTATCA TCGGATCCTT CTTTGTCCTT 
AATCTTGTCC TTGGAGTGCT GTCCGGTGAA TTTGCCAAAG AGAGGGAAAG AGTGGAAAAC 
CGCCGGGCTT TCATGAAGTT GCGCAGGCAG CAGCAGATTG AGAGGGAGTT GAATGGCTAT 
CGGGCATGGA TAGATAAAGC TGAGGAAGTC ATGTTGGCAG AAGAAAACAA AAATTCTGGG 
ACCTCTGCTT TAGAAGTTCT TAGGCGAGCA ACCATTAAAC GAAGTAGAAC AGAAGCCATG 
AACCGGGACT CTACTGAGGA CCATTGTGTT GACATATCCT CTGTTGGCAC CCCCCTTGCT 
AGAGCAAGCA TCAAAAGTAC CAAAATGGAT GGGGCATCAT ATTTTCGGCA CAAGGAAAAA 
CTATTGCGTG TCTCCGTTCG TCACATGATC AAATCCCAGG TTTTCTACTG GATGGTCCTG 
GGAGTGGTAG CACTTAACAC TTTCTGTGTG GCTATTGTCC ATCACAACCA GACCCTGTGG 
CTCTCAAATG TTCTTTACTA TGCAGAATTT CTGTTTCTTG GGTTATTTTT GGTGGAGATG 
GCTTTTAAAA TGTATGGAAT GGGTCCACGT CTTTACTTTC ACTCATCCTT CAACTGCTTT 
GATTGCGGGG TCACAATAGG TAGTATATTT GAAGTACTTT GGGCCATATT TAGACCAGGA 
ACTTCCTTTG GAATCAGTGT CTTAAGAGCT CTCCGACTTT TGAGAATCTT CAAAATAACA 
AAATACTGGG CCTCACTACG AAACCTTGTA GTATCATTGA TGAATTCTAT GAAATCCATC 
ATCAGCCTGC TCTTCCTTCT ATTCCTCTTC ATAGTTGTTT TTGCTCTCCT TGGAATGCAG 
TTATATGGTG GCAGATTCAA TTTTAATGAT GGCACTCCTT CTGCAAACTT TGATACATTC 
CCTGCAGCCA TCATGACTGT ATTCCAGATT CTGACAGGAG AGGATTGGAA TGAAGTGATG 
TACAATGGTA TTCGCTCCCA AGGTGGTGTT CGATCCGGAA TGTGGTCTTC CATATACTTT 
ATTGTCCTTA CCCTTTTTGG AAATTACACA CTGCTAAATG TATTTCTTGC CATTGCTGTG 
GACAACCTGG CTAATGCACA GGAGTTAACT AAGGATGAGC AAGAAGAAGA GGAAGCTTTC 
AATCAGAAGC ATGCACTGCA AAAAGCGAAG GAGGTCAGCC CCATGTCTGC ACCTAATGCA 
TCATGTATTG AGAAGGAGCG CAGAAAACGG CACACATTGT CTATATGGGA GCAGCGCACA 
AGCCAGCTAA GGAGGCACAT ACAGATGTCT AGTCAAGAAG CTCTCCAGAG TGAAGAACTG 
GCAGCATTGA ATCCACACTC CAGTATCTAT CGCAAGACAA AGGAAGCTGC TGAACAAAAC 
ACAGAAAAAG AAGAAGTGCA TCAAGACGCA AAACTGGCAA TAGACCCAGT TGAAGGACCA 
CCAGAAGGTG AAGGAAGCCC TTCTAATAGA AATCTCAGGG AAAACTGGCG CCAGAAGTCC 
AGGCATGGAA ATTCTGAAAA CACTGAGCCA AATGGAACAA CTCCAGAAGA AGCAATGCGC 
CGCAGACAAA GTCAGAGACG CAGCCGTCAT AGAAGAGTTA GAACTGAAGG TCGTGAGCAG 
TGTAGCAATC CAAACAGTAC CTCAGCAAGC AGAGAAGGTG GTCTTGACAA AGTCTCTGCA 
GAGGATGGAA GAACAATTCC AGAAGAGAAG AGCAATGGAG AAGCCAAAGA AGTCAAAGGG 
GTTGATGATG AGCCTCTAAA GATTTGTATA TCTTCAAGAA ATTGCGAGGA CAGAAATATC 
AGGACTTTGG GTTGTGAGGG TATTGCCTTG AACGATACAC CCCTGCAGGA TGTGAACAGC 
CAAGAGAAGG ACAGTAGCCC TCCTGATTTG GATTGCAGTG AGCAGGCTCT GTTGGGCAGC 
ATTCAGAGCC AGGAAAGCCC TAATCCAACC AGCTGCAGTT TGCCAGATGT GTCCAGCAGC 
ACCGAGAGAG TCACAGAGAG CACCGTCATT ACCTTACCAG ATACTGGAAT GCCCACCGAC 
TGCACCGTGG TACAGATGAG TGAAGGCAGG GATGGAGAAG TCAACTTTGG GTCTTCGCAG 
CAAGAAAAAG AAGAGGAGGA AGCTGAGGAT GGAGAAGAAA GAACTATAAA AAGAAAAGAG 
AAATTGTTGG AGTCCCCCAA AGCCATTGTA CCGTACAGTT CAATGCTCAT CTTCAGCTCC 
ACCAATCCCA TAAGAAAAGT ATGTCATTAC ATTGTTAACC TACGATACTT TGAGATGAGC 
ATCCTGCTGG TAATAGCAGC AAGTAGTATA GCCTTAGCAG CGGAGGACCC TGTACTGACC 
AACTCGGACC GCAACAAAGT TCTTCGCTAT TTTGACTATG TTTTCACAGG GGTCTTCACT 
TTTGAGATGA TTATAAAGAT GATTGACCAA GGTTTGATTT TACACGAGGG CTCCTACTTT 
CGTGACCTAT GGAACATCTT GGATTTTGTT GTTGTTGTGG GAGCCTTAAT TGCGTTTGCC 
TTAGCGAATG TTTTGGGGAC TAACAAAGGC CGGGACATCA AAACCATTAA GTCTCTACGT 
GTGTTACGGG TGCTGCGTCC ACTAAAAACA ATCAAACGCC TGCCAAAACT GAAGGCAGTC 
TTTGATTGTG TGGTTAACTC TTTAAAGAAT GTCTTTAATA TTCTCATCGT ATACAAACTC 
TTCATGTTCA TATTTGCTGT AATCGCTGTC CAGCTGTTTA AGGGCAAGTT CTTCTATTGT 
ACAGACAGCT CAAAGGAGAC AGAGAAAGAG TGCATAGGGA GTTACATCGA TCACGAAAAG 
AACAAGATGG AGGTGAAGCG GAGAGAGTGG AAAAGACATG ATTTCCATTA TGACAACATA 
ATATGGGCTC TTCTTACCCT CTTTACTGTA TCAACAGGGG AAGGCTGGCC TCAGGTTCTG 
CAGCACTCTG TTGATATAAC TGAAGAAGAT AGGGGTCCAA GCCGAAGCAA CCGTATGGAG 
ATGTCCATTT TCTATGTTAT CTACTTTGTG GTCTTCCCCT TCTTCTTTGT AAATATATTT 
GTGGCTCTGA TTATCATCAC ATTCCAGGAG CAAGGAGACA AGATGATGGA GGAATGTTCC 
CTAGAAAAGA ATGAGAGAGC CTGCATAGAC TTTGCAATAA GTGCTAAGCC ACTTACACGA 
TATATGCCAC AGAACAGGCA CACATTCCAG TACCGGGTCT GGCACTTCGT GGTCTCCCCA 
TCATTTGAAT ACACTATCAT GGCTATGATT GCACTAAACA CAGTGGTACT CATGATGAAG 
TATTATTCTG CGCCATACAC ATATGAGCTT GCATTAAAGT ATCTCAATAT TGCATTCACA 
ATGCTATTTT CTCTGGAGTG CATCCTGAAA ATAATTGCAT TTGGGTTTTT GAATTACTTT 
CGGGATACTT GGAACATTTT TGACTTCATT ACTGTAATTG GTAGCATCAC GGAAATTATT 
CTAACAGATA GTAAGTTGGT GAACACCAGC AGCTTTAATA TGAGCTTTCT GAAGCTGTTC 
CGAGCGGCGC GACTTATTAA ACTTCTCCGG CAAGGGTACA CCATCCGGAT CCTGCTGTGG 
ACTTTTGTTC AGTCCTTTAA GGCACTTCCT TATGTGTGTC TTCTCATTGC AATGTTGTTC 
TTCATCTATG CCATTATTGG GATGCAGGTG TTTGGCAATA TCCGCCTGGA AGAGGACAGT 
CATATTAATA GGCACAACAA TTTTAGAAGC TTCTTTAGTT CTCTTATGTT ACTCTTCAGG 
AGTGCCACAG GAGAAGCGTG GCAGGAGATC ATGCTTTCAT GCCTTAGTGA TAAGGGTTGT 
GAGCCAGACA CCAGTGCAGC CTCTGGACAC AACGAGAATG AGCGCTGTGG AACTGAACTT 
GCATATGTTT ATTTTGTTTC ATTCATTTTC TTCTGCTCCT TCCTCATGCT AAATCTGTTT 
GTGGCTGTCA TTATGGACAA CTTTGAGTAC TTGACACGGG ACTCTTCCAT TTTGGGACCT 
CATCACTTGG ATGAGTTTGT TCGGATATGG GCTGAATATG ATCGAGCAGC ATGTGGCCGT 
ATCCATTACA CAGAGATGTA TGACATGCTG ACTCTCATGT CACCACCTCT AGGCCTTGGA 
AAGAGATGCC CTAACAAAGT GGCTTATAAG AGATTGGTCC TGATGAATAT GCCAGTGTCG 
GAGGACATGA CGGTTCATTT CACATCAACT CTTATGGCCC TAATACGCAC GGCACTGGAT 
ATCAAAATAG CTAAAGGAGG TGCTGACAGG CAACAGCTGG ACTCAGAGCT GCAGAAAGAT 
ATACTTGCCA TTTGGCCAAA TCTCTCCCAG AAAATGATCG ACTTACTTGT GCCAATGCCA 
AGAGCCTCTG ATCTGACGGT GGGGAAGATT TACGCTGCAA TGATGATCAT GGATTACTAT 
AAACAGAGCA AAGCGAAAAA ACAAAGGCAG CAGCTGGAGG AGCAGAAAAA TGCACCTATG 
TTTCAGAGAA TGGAGGCTTC TGCCCTCCCT GAGGAGATCT TATCCAATGC CAAAGCTTTA 
CCATACCTTC AGCAAGACAC TCTATCAGGC CTGGGTGGAT TTCCTTCACT GAGTCCCTTG 
TCCCCAGAAG AAGTATTCCA GCCGCCATGC CTAGACGGGG AAGAGGAGGA AGACTTTGAA 
GAGCAGCTGT CTCTGGAGCC TGAGGTTAGA GAGTTTAGAA GGGAGCAGCT CCCTAACCGA 
GGCAGCTACA TCCCTGTGGA GATGCTGGAA CGTCCAGCGT CTGTGAGAGC TTCTTCCATG 
CCACGTCTGG CTGAGAATCC TCAGGTCCTG TCAGACGTAA GCTCCATGCG TCGCTCCTTC 
TCCACAACTG CAGAGAAGCG AGTGCGCAGC TCGTGGCTAG ATGAGTTCAC TATGGACAGG 
AGTAGCACTG ACTGTGCATA CAGATACCAG AGGCGCAGTT ATCATGCATC CTTAAGACTG 
TCTGCACATC GCCTGAATAC AGATACAGGT CACTGGTCAG ATAATCACCG TTCTGGCGGA 
AGGGAGCGAG GAAGGTCCAA AGAGAGAAAA CACTTGCTGT CACCCGATCC TTCCCGCTGT 
AATTCTGAGG AAAGGAGCCC ACAAGCTGCC TGTGAACACC GAGATCACCA ACTGTCCCCT 
GGGCATACTC CCAGTAGTGT ACAGATTCTT GACTCTCTGA GTGAAAGTTG TACCCCTTCA 
GACACCAGCA CCCCAAGAAG AGGCCGTAGA CAGCTTCCTT CTGTGCCTCC AACTCCTAGA 
CCCCTTATCT CTTTCTCCTC GTTAATCCGC CGGCAGAAAC ACAGCCCCCC TCCTCAAAAT 
AAAAATGATA CTATCCTTCC TAGTGAGCCT GGTCTGACTG AGTCTTCCTG CTCCCTTGGG 
GGTCATCAGA GCTCCCAGTC CACACCACAG CATTATATCT CAGAGCCCTA CCTGGCACTG 
CATGAGGAGA TGCAAAGCTC AGACTGCCCA GCAGAGGACA CACTCACCTT TGAGGTTGCT 
GTGGCTACTA GTCTGGGCCG CTCGAACACC ATTGGCTCTG CCCCTCTACG TCATGGCTGG 
CAAATGCCCA ATGGTCACTA CCGGCGTCGA AGGAAGGGAG GAATTCCACC AGTGATACCC 
TCCAGAGAGT TAAGTGACAC AGAAGAGGAT GATCGATGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1e ( XM_018093597.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949086.2 Xenopus tropicalis calcium channel, voltage-dependent, R type, alpha 1E subunit (cacna1e), transcript variant X2, mRNA.

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CloneID	OXa57230
Clone ID Related Accession (Same CDS sequence)	XM_018093597.2
Accession Version	XM_018093597.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7182bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018093597.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTATTAA AGATGCTGGG ATTAAGCTCC TGTTCAACCA GTGCTCATAC ACTCAATGAG 
CCTGACCAGT CAAGCACACT AGCCATACCA GCATTGGATG CCGACACTCA GAGATATGTC 
TTGGCCCTTC CAGAAGCAGA GACATGGCGC AAGCATCGAT TGGGCCTGAC ACAGGCTACT 
CACTCTTGCA ATCTTCTAGA ACCTGAGACA CTTTCTGAAA CCCTAGTTTC ACGCACTGCA 
AGTTACGATG CCTTGTATGA CCTGCGAGCA AGAGAAGCGG AGTCTAACTT CCCATTTGAA 
GGCAGCAGCA TGTTTGAGGT GACGTCTGGG ATGGATTCTG AGTTTCCTTT AAGCCCCCAT 
GTTATAAAGC GAAGGAGAGG AGGCCTGATA GAACAAAAGG ATATCATCAA GGCCCACGAA 
GCTCATAAGA TTCAGAGTAC TCCCCAGGCT CGACGGAAGG AATGGGAGAT GGCTCGTTTC 
GGAGAGGCTG TGACTGGCAG GTTGAGCTCC GCAGATGGGG GCTCAGAGAG GAGCAAGACC 
CTACAAGGTT CCCCAGCTGC ACCTGTTGGG ACCCAAGCAG CTTTCAAGCA ATCCAAAGCC 
CAGAGGGCAA GGACCATGGC TTTATACAAC CCCATCCCTG TCCGACAGAA CTGCTTCACC 
GTGAACAGGT CTCTCTTCAT TTTCGGAGAA GATAATATTA TCAGGAAATA CGCCAAGAAG 
CTCATTGATT GGCCTCCTTT TGAATACATG ATCCTTGCCA CAATTATTGC AAACTGCATT 
GTCCTGGCTT TGGAGCAGCA TCTTCCAGGC GATGACAAGA CTCCAATGTC TCGCAGATTG 
GAAAAGACTG AGCCCTATTT CATTGGGATA TTTTGCTTTG AAGCTGGAAT TAAAATTGTC 
GCTCTGGGAC TGGTTTTTCA CAAAGGTTCT TATCTTCGGA ATGGCTGGAA TGTCATGGAC 
TTTATAGTAG TGCTCAGTGG CATCCTGGCC ACAGCTGGAA CTCACTTCAA TACTCACGTG 
GACCTGCGTA CTTTGCGGGC AGTGCGTGTT CTCAGGCCGT TGAAGCTGGT CTCGGGGATT 
CCCAGTTTAC AGATTGTGTT GAAGTCAATC ATGAAGGCGA TGGTCCCTCT GCTACAGATT 
GGATTGTTGC TCTTCTTTGC CATCCTTATG TTTGCTATCA TCGGCCTTGA GTTTTACAGT 
GGAAAGCTAC ACAAGGCCTG TTACAGGAAC AACTCTGGAA TGGAGCTAGA CCCCATGGAT 
CCACCTCACC CTTGTGGAGT GCCCCGCTGC CCAGCTGGGT ATGAGTGCAA GGAATGGATC 
GGACCCAATG ATGGAATAAC ACAGTTTGAC AACATCCTAT TTGCGGTGCT GACAGTCTTC 
CAGTGTATAA CTATGGAGGG CTGGACCACA GTGCTGTACA ATACCAATGA TGCCTTAGGA 
GCCACATGGA ACTGGCTGTA CTTCATTCCC CTCATTATCA TCGGATCCTT CTTTGTCCTT 
AATCTTGTCC TTGGAGTGCT GTCCGGTGAA TTTGCCAAAG AGAGGGAAAG AGTGGAAAAC 
CGCCGGGCTT TCATGAAGTT GCGCAGGCAG CAGCAGATTG AGAGGGAGTT GAATGGCTAT 
CGGGCATGGA TAGATAAAGC TGAGGAAGTC ATGTTGGCAG AAGAAAACAA AAATTCTGGG 
ACCTCTGCTT TAGAAGTTCT TAGGCGAGCA ACCATTAAAC GAAGTAGAAC AGAAGCCATG 
AACCGGGACT CTACTGAGGA CCATTGTGTT GACATATCCT CTGTTGGCAC CCCCCTTGCT 
AGAGCAAGCA TCAAAAGTAC CAAAATGGAT GGGGCATCAT ATTTTCGGCA CAAGGAAAAA 
CTATTGCGTG TCTCCGTTCG TCACATGATC AAATCCCAGG TTTTCTACTG GATGGTCCTG 
GGAGTGGTAG CACTTAACAC TTTCTGTGTG GCTATTGTCC ATCACAACCA GACCCTGTGG 
CTCTCAAATG TTCTTTACTA TGCAGAATTT CTGTTTCTTG GGTTATTTTT GGTGGAGATG 
GCTTTTAAAA TGTATGGAAT GGGTCCACGT CTTTACTTTC ACTCATCCTT CAACTGCTTT 
GATTGCGGGG TCACAATAGG TAGTATATTT GAAGTACTTT GGGCCATATT TAGACCAGGA 
ACTTCCTTTG GAATCAGTGT CTTAAGAGCT CTCCGACTTT TGAGAATCTT CAAAATAACA 
AAATACTGGG CCTCACTACG AAACCTTGTA GTATCATTGA TGAATTCTAT GAAATCCATC 
ATCAGCCTGC TCTTCCTTCT ATTCCTCTTC ATAGTTGTTT TTGCTCTCCT TGGAATGCAG 
TTATATGGTG GCAGATTCAA TTTTAATGAT GGCACTCCTT CTGCAAACTT TGATACATTC 
CCTGCAGCCA TCATGACTGT ATTCCAGATT CTGACAGGAG AGGATTGGAA TGAAGTGATG 
TACAATGGTA TTCGCTCCCA AGGTGGTGTT CGATCCGGAA TGTGGTCTTC CATATACTTT 
ATTGTCCTTA CCCTTTTTGG AAATTACACA CTGCTAAATG TATTTCTTGC CATTGCTGTG 
GACAACCTGG CTAATGCACA GGAGTTAACT AAGGATGAGC AAGAAGAAGA GGAAGCTTTC 
AATCAGAAGC ATGCACTGCA AAAAGCGAAG GAGGTCAGCC CCATGTCTGC ACCTAATGCA 
TCATGTATTG AGAAGGAGCG CAGAAAACGG CACACATTGT CTATATGGGA GCAGCGCACA 
AGCCAGCTAA GGAGGCACAT ACAGATGTCT AGTCAAGAAG CTCTCCAGAG TGAAGAACTG 
GCAGCATTGA ATCCACACTC CAGTATCTAT CGCAAGACAA AGGAAGCTGC TGAACAAAAC 
ACAGAAAAAG AAGAAGTGCA TCAAGACGCA AAACTGGCAA TAGACCCAGT TGAAGGACCA 
CCAGAAGGTG AAGGAAGCCC TTCTAATAGA AATCTCAGGG AAAACTGGCG CCAGAAGTCC 
AGGCATGGAA ATTCTGAAAA CACTGAGCCA AATGGAACAA CTCCAGAAGA AGCAATGCGC 
CGCAGACAAA GTCAGAGACG CAGCCGTCAT AGAAGAGTTA GAACTGAAGG TCGTGAGCAG 
TGTAGCAATC CAAACAGTAC CTCAGCAAGC AGAGAAGGTG GTCTTGACAA AGTCTCTGCA 
GAGGATGGAA GAACAATTCC AGAAGAGAAG AGCAATGGAG AAGCCAAAGA AGTCAAAGGG 
GTTGATGATG AGCCTCTAAA GATTTGTATA TCTTCAAGAA ATTGCGAGGA CAGAAATATC 
AGGACTTTGG GTTGTGAGGG TATTGCCTTG AACGATACAC CCCTGCAGGA TGTGAACAGC 
CAAGAGAAGG ACAGTAGCCC TCCTGATTTG GATTGCAGTG AGCAGGCTCT GTTGGGCAGC 
ATTCAGAGCC AGGAAAGCCC TAATCCAACC AGCTGCAGTT TGCCAGATGT GTCCAGCAGC 
ACCGAGAGAG TCACAGAGAG CACCGTCATT ACCTTACCAG ATACTGGAAT GCCCACCGAC 
TGCACCGTGG TACAGATGAG TGAAGGCAGG GATGGAGAAG TCAACTTTGG GTCTTCGCAG 
CAAGAAAAAG AAGAGGAGGA AGCTGAGGAT GGAGAAGAAA GAACTATAAA AAGAAAAGAG 
AAATTGTTGG AGTCCCCCAA AGCCATTGTA CCGTACAGTT CAATGCTCAT CTTCAGCTCC 
ACCAATCCCA TAAGAAAAGT ATGTCATTAC ATTGTTAACC TACGATACTT TGAGATGAGC 
ATCCTGCTGG TAATAGCAGC AAGTAGTATA GCCTTAGCAG CGGAGGACCC TGTACTGACC 
AACTCGGACC GCAACAAAGT TCTTCGCTAT TTTGACTATG TTTTCACAGG GGTCTTCACT 
TTTGAGATGA TTATAAAGAT GATTGACCAA GGTTTGATTT TACACGAGGG CTCCTACTTT 
CGTGACCTAT GGAACATCTT GGATTTTGTT GTTGTTGTGG GAGCCTTAAT TGCGTTTGCC 
TTAGCGAATG TTTTGGGGAC TAACAAAGGC CGGGACATCA AAACCATTAA GTCTCTACGT 
GTGTTACGGG TGCTGCGTCC ACTAAAAACA ATCAAACGCC TGCCAAAACT GAAGGCAGTC 
TTTGATTGTG TGGTTAACTC TTTAAAGAAT GTCTTTAATA TTCTCATCGT ATACAAACTC 
TTCATGTTCA TATTTGCTGT AATCGCTGTC CAGCTGTTTA AGGGCAAGTT CTTCTATTGT 
ACAGACAGCT CAAAGGAGAC AGAGAAAGAG TGCATAGGGA GTTACATCGA TCACGAAAAG 
AACAAGATGG AGGTGAAGCG GAGAGAGTGG AAAAGACATG ATTTCCATTA TGACAACATA 
ATATGGGCTC TTCTTACCCT CTTTACTGTA TCAACAGGGG AAGGCTGGCC TCAGGTTCTG 
CAGCACTCTG TTGATATAAC TGAAGAAGAT AGGGGTCCAA GCCGAAGCAA CCGTATGGAG 
ATGTCCATTT TCTATGTTAT CTACTTTGTG GTCTTCCCCT TCTTCTTTGT AAATATATTT 
GTGGCTCTGA TTATCATCAC ATTCCAGGAG CAAGGAGACA AGATGATGGA GGAATGTTCC 
CTAGAAAAGA ATGAGAGAGC CTGCATAGAC TTTGCAATAA GTGCTAAGCC ACTTACACGA 
TATATGCCAC AGAACAGGCA CACATTCCAG TACCGGGTCT GGCACTTCGT GGTCTCCCCA 
TCATTTGAAT ACACTATCAT GGCTATGATT GCACTAAACA CAGTGGTACT CATGATGAAG 
TATTATTCTG CGCCATACAC ATATGAGCTT GCATTAAAGT ATCTCAATAT TGCATTCACA 
ATGCTATTTT CTCTGGAGTG CATCCTGAAA ATAATTGCAT TTGGGTTTTT GAATTACTTT 
CGGGATACTT GGAACATTTT TGACTTCATT ACTGTAATTG GTAGCATCAC GGAAATTATT 
CTAACAGATA GTAAGTTGGT GAACACCAGC AGCTTTAATA TGAGCTTTCT GAAGCTGTTC 
CGAGCGGCGC GACTTATTAA ACTTCTCCGG CAAGGGTACA CCATCCGGAT CCTGCTGTGG 
ACTTTTGTTC AGTCCTTTAA GGCACTTCCT TATGTGTGTC TTCTCATTGC AATGTTGTTC 
TTCATCTATG CCATTATTGG GATGCAGGTG TTTGGCAATA TCCGCCTGGA AGAGGACAGT 
CATATTAATA GGCACAACAA TTTTAGAAGC TTCTTTAGTT CTCTTATGTT ACTCTTCAGG 
AGTGCCACAG GAGAAGCGTG GCAGGAGATC ATGCTTTCAT GCCTTAGTGA TAAGGGTTGT 
GAGCCAGACA CCAGTGCAGC CTCTGGACAC AACGAGAATG AGCGCTGTGG AACTGAACTT 
GCATATGTTT ATTTTGTTTC ATTCATTTTC TTCTGCTCCT TCCTCATGCT AAATCTGTTT 
GTGGCTGTCA TTATGGACAA CTTTGAGTAC TTGACACGGG ACTCTTCCAT TTTGGGACCT 
CATCACTTGG ATGAGTTTGT TCGGATATGG GCTGAATATG ATCGAGCAGC ATGTGGCAGG 
ATCCCCTATA AGGATATGTA CAAATTAGTC CGAGTCATCT CGCCTCCGCT CGGCCTAGGA 
GAGAACTGCC CATACAGGGT GGCATGCAAG AGATTGGTCC TGATGAATAT GCCAGTGTCG 
GAGGACATGA CGGTTCATTT CACATCAACT CTTATGGCCC TAATACGCAC GGCACTGGAT 
ATCAAAATAG CTAAAGGAGG TGCTGACAGG CAACAGCTGG ACTCAGAGCT GCAGAAAGAT 
ATACTTGCCA TTTGGCCAAA TCTCTCCCAG AAAATGATCG ACTTACTTGT GCCAATGCCA 
AGAGCCTCTG ATCTGACGGT GGGGAAGATT TACGCTGCAA TGATGATCAT GGATTACTAT 
AAACAGAGCA AAGCGAAAAA ACAAAGGCAG CAGCTGGAGG AGCAGAAAAA TGCACCTATG 
TTTCAGAGAA TGGAGGCTTC TGCCCTCCCT GAGGAGATCT TATCCAATGC CAAAGCTTTA 
CCATACCTTC AGCAAGACAC TCTATCAGGC CTGGGTGGAT TTCCTTCACT GAGTCCCTTG 
TCCCCAGAAG AAGTATTCCA GCCGCCATGC CTAGACGGGG AAGAGGAGGA AGACTTTGAA 
GAGCAGCTGT CTCTGGAGCC TGAGGTTAGA GAGTTTAGAA GGGAGCAGCT CCCTAACCGA 
GGCAGCTACA TCCCTGTGGA GATGCTGGAA CGTCCAGCGT CTGTGAGAGC TTCTTCCATG 
CCACGTCTGG CTGAGAATCC TCAGGTCCTG TCAGACGTAA GCTCCATGCG TCGCTCCTTC 
TCCACAACTG CAGAGAAGCG AGTGCGCAGC TCGTGGCTAG ATGAGTTCAC TATGGACAGG 
AGTAGCACTG ACTGTGCATA CAGATACCAG AGGCGCAGTT ATCATGCATC CTTAAGACTG 
TCTGCACATC GCCTGAATAC AGATACAGGT CACTGGTCAG ATAATCACCG TTCTGGCGGA 
AGGGAGCGAG GAAGGTCCAA AGAGAGAAAA CACTTGCTGT CACCCGATCC TTCCCGCTGT 
AATTCTGAGG AAAGGAGCCC ACAAGCTGCC TGTGAACACC GAGATCACCA ACTGTCCCCT 
GGGCATACTC CCAGTAGTGT ACAGATTCTT GACTCTCTGA GTGAAAGTTG TACCCCTTCA 
GACACCAGCA CCCCAAGAAG AGGCCGTAGA CAGCTTCCTT CTGTGCCTCC AACTCCTAGA 
CCCCTTATCT CTTTCTCCTC GTTAATCCGC CGGCAGAAAC ACAGCCCCCC TCCTCAAAAT 
AAAAATGATA CTATCCTTCC TAGTGAGCCT GGTCTGACTG AGTCTTCCTG CTCCCTTGGG 
GGTCATCAGA GCTCCCAGTC CACACCACAG CATTATATCT CAGAGCCCTA CCTGGCACTG 
CATGAGGAGA TGCAAAGCTC AGACTGCCCA GCAGAGGACA CACTCACCTT TGAGGTTGCT 
GTGGCTACTA GTCTGGGCCG CTCGAACACC ATTGGCTCTG CCCCTCTACG TCATGGCTGG 
CAAATGCCCA ATGGTCACTA CCGGCGTCGA AGGAAGGGAG GAATTCCACC AGTGATACCC 
TCCAGAGAGT TAAGTGACAC AGAAGAGGAT GATCGATGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949086.2
CDS	285..7466
Translation	MVLKMLGLSSCSTSAHTLNEPDQSSTLAIPALDADTQRYVLALPEAETWRKHRLGLTQATHSCNLLEPETLSETLVSRTASYDALYDLRAREAESNFPFEGSSMFEVTSGMDSEFPLSPHVIKRRRGGLIEQKDIIKAHEAHKIQSTPQARRKEWEMARFGEAVTGRLSSADGGSERSKTLQGSPAAPVGTQAAFKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIIRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPGDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGLVFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHKACYRNNSGMELDPMDPPHPCGVPRCPAGYECKEWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNSGTSALEVLRRATIKRSRTEAMNRDSTEDHCVDISSVGTPLARASIKSTKMDGASYFRHKEKLLRVSVRHMIKSQVFYWMVLGVVALNTFCVAIVHHNQTLWLSNVLYYAEFLFLGLFLVEMAFKMYGMGPRLYFHSSFNCFDCGVTIGSIFEVLWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMNSMKSIISLLFLLFLFIVVFALLGMQLYGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVRSGMWSSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNASCIEKERRKRHTLSIWEQRTSQLRRHIQMSSQEALQSEELAALNPHSSIYRKTKEAAEQNTEKEEVHQDAKLAIDPVEGPPEGEGSPSNRNLRENWRQKSRHGNSENTEPNGTTPEEAMRRRQSQRRSRHRRVRTEGREQCSNPNSTSASREGGLDKVSAEDGRTIPEEKSNGEAKEVKGVDDEPLKICISSRNCEDRNIRTLGCEGIALNDTPLQDVNSQEKDSSPPDLDCSEQALLGSIQSQESPNPTSCSLPDVSSSTERVTESTVITLPDTGMPTDCTVVQMSEGRDGEVNFGSSQQEKEEEEAEDGEERTIKRKEKLLESPKAIVPYSSMLIFSSTNPIRKVCHYIVNLRYFEMSILLVIAASSIALAAEDPVLTNSDRNKVLRYFDYVFTGVFTFEMIIKMIDQGLILHEGSYFRDLWNILDFVVVVGALIAFALANVLGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKETEKECIGSYIDHEKNKMEVKRREWKRHDFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDITEEDRGPSRSNRMEMSIFYVIYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPYTYELALKYLNIAFTMLFSLECILKIIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSSFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIRLEEDSHINRHNNFRSFFSSLMLLFRSATGEAWQEIMLSCLSDKGCEPDTSAASGHNENERCGTELAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRIWAEYDRAACGRIPYKDMYKLVRVISPPLGLGENCPYRVACKRLVLMNMPVSEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKDILAIWPNLSQKMIDLLVPMPRASDLTVGKIYAAMMIMDYYKQSKAKKQRQQLEEQKNAPMFQRMEASALPEEILSNAKALPYLQQDTLSGLGGFPSLSPLSPEEVFQPPCLDGEEEEDFEEQLSLEPEVREFRREQLPNRGSYIPVEMLERPASVRASSMPRLAENPQVLSDVSSMRRSFSTTAEKRVRSSWLDEFTMDRSSTDCAYRYQRRSYHASLRLSAHRLNTDTGHWSDNHRSGGRERGRSKERKHLLSPDPSRCNSEERSPQAACEHRDHQLSPGHTPSSVQILDSLSESCTPSDTSTPRRGRRQLPSVPPTPRPLISFSSLIRRQKHSPPPQNKNDTILPSEPGLTESSCSLGGHQSSQSTPQHYISEPYLALHEEMQSSDCPAEDTLTFEVAVATSLGRSNTIGSAPLRHGWQMPNGHYRRRRKGGIPPVIPSRELSDTEEDDRC

Target ORF information:

RefSeq Version	XM_018093597.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calcium channel, voltage-dependent, R type, alpha 1E subunit (cacna1e), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018093597.2

ORF Insert Sequence:

ATGGTATTAA AGATGCTGGG ATTAAGCTCC TGTTCAACCA GTGCTCATAC ACTCAATGAG 
CCTGACCAGT CAAGCACACT AGCCATACCA GCATTGGATG CCGACACTCA GAGATATGTC 
TTGGCCCTTC CAGAAGCAGA GACATGGCGC AAGCATCGAT TGGGCCTGAC ACAGGCTACT 
CACTCTTGCA ATCTTCTAGA ACCTGAGACA CTTTCTGAAA CCCTAGTTTC ACGCACTGCA 
AGTTACGATG CCTTGTATGA CCTGCGAGCA AGAGAAGCGG AGTCTAACTT CCCATTTGAA 
GGCAGCAGCA TGTTTGAGGT GACGTCTGGG ATGGATTCTG AGTTTCCTTT AAGCCCCCAT 
GTTATAAAGC GAAGGAGAGG AGGCCTGATA GAACAAAAGG ATATCATCAA GGCCCACGAA 
GCTCATAAGA TTCAGAGTAC TCCCCAGGCT CGACGGAAGG AATGGGAGAT GGCTCGTTTC 
GGAGAGGCTG TGACTGGCAG GTTGAGCTCC GCAGATGGGG GCTCAGAGAG GAGCAAGACC 
CTACAAGGTT CCCCAGCTGC ACCTGTTGGG ACCCAAGCAG CTTTCAAGCA ATCCAAAGCC 
CAGAGGGCAA GGACCATGGC TTTATACAAC CCCATCCCTG TCCGACAGAA CTGCTTCACC 
GTGAACAGGT CTCTCTTCAT TTTCGGAGAA GATAATATTA TCAGGAAATA CGCCAAGAAG 
CTCATTGATT GGCCTCCTTT TGAATACATG ATCCTTGCCA CAATTATTGC AAACTGCATT 
GTCCTGGCTT TGGAGCAGCA TCTTCCAGGC GATGACAAGA CTCCAATGTC TCGCAGATTG 
GAAAAGACTG AGCCCTATTT CATTGGGATA TTTTGCTTTG AAGCTGGAAT TAAAATTGTC 
GCTCTGGGAC TGGTTTTTCA CAAAGGTTCT TATCTTCGGA ATGGCTGGAA TGTCATGGAC 
TTTATAGTAG TGCTCAGTGG CATCCTGGCC ACAGCTGGAA CTCACTTCAA TACTCACGTG 
GACCTGCGTA CTTTGCGGGC AGTGCGTGTT CTCAGGCCGT TGAAGCTGGT CTCGGGGATT 
CCCAGTTTAC AGATTGTGTT GAAGTCAATC ATGAAGGCGA TGGTCCCTCT GCTACAGATT 
GGATTGTTGC TCTTCTTTGC CATCCTTATG TTTGCTATCA TCGGCCTTGA GTTTTACAGT 
GGAAAGCTAC ACAAGGCCTG TTACAGGAAC AACTCTGGAA TGGAGCTAGA CCCCATGGAT 
CCACCTCACC CTTGTGGAGT GCCCCGCTGC CCAGCTGGGT ATGAGTGCAA GGAATGGATC 
GGACCCAATG ATGGAATAAC ACAGTTTGAC AACATCCTAT TTGCGGTGCT GACAGTCTTC 
CAGTGTATAA CTATGGAGGG CTGGACCACA GTGCTGTACA ATACCAATGA TGCCTTAGGA 
GCCACATGGA ACTGGCTGTA CTTCATTCCC CTCATTATCA TCGGATCCTT CTTTGTCCTT 
AATCTTGTCC TTGGAGTGCT GTCCGGTGAA TTTGCCAAAG AGAGGGAAAG AGTGGAAAAC 
CGCCGGGCTT TCATGAAGTT GCGCAGGCAG CAGCAGATTG AGAGGGAGTT GAATGGCTAT 
CGGGCATGGA TAGATAAAGC TGAGGAAGTC ATGTTGGCAG AAGAAAACAA AAATTCTGGG 
ACCTCTGCTT TAGAAGTTCT TAGGCGAGCA ACCATTAAAC GAAGTAGAAC AGAAGCCATG 
AACCGGGACT CTACTGAGGA CCATTGTGTT GACATATCCT CTGTTGGCAC CCCCCTTGCT 
AGAGCAAGCA TCAAAAGTAC CAAAATGGAT GGGGCATCAT ATTTTCGGCA CAAGGAAAAA 
CTATTGCGTG TCTCCGTTCG TCACATGATC AAATCCCAGG TTTTCTACTG GATGGTCCTG 
GGAGTGGTAG CACTTAACAC TTTCTGTGTG GCTATTGTCC ATCACAACCA GACCCTGTGG 
CTCTCAAATG TTCTTTACTA TGCAGAATTT CTGTTTCTTG GGTTATTTTT GGTGGAGATG 
GCTTTTAAAA TGTATGGAAT GGGTCCACGT CTTTACTTTC ACTCATCCTT CAACTGCTTT 
GATTGCGGGG TCACAATAGG TAGTATATTT GAAGTACTTT GGGCCATATT TAGACCAGGA 
ACTTCCTTTG GAATCAGTGT CTTAAGAGCT CTCCGACTTT TGAGAATCTT CAAAATAACA 
AAATACTGGG CCTCACTACG AAACCTTGTA GTATCATTGA TGAATTCTAT GAAATCCATC 
ATCAGCCTGC TCTTCCTTCT ATTCCTCTTC ATAGTTGTTT TTGCTCTCCT TGGAATGCAG 
TTATATGGTG GCAGATTCAA TTTTAATGAT GGCACTCCTT CTGCAAACTT TGATACATTC 
CCTGCAGCCA TCATGACTGT ATTCCAGATT CTGACAGGAG AGGATTGGAA TGAAGTGATG 
TACAATGGTA TTCGCTCCCA AGGTGGTGTT CGATCCGGAA TGTGGTCTTC CATATACTTT 
ATTGTCCTTA CCCTTTTTGG AAATTACACA CTGCTAAATG TATTTCTTGC CATTGCTGTG 
GACAACCTGG CTAATGCACA GGAGTTAACT AAGGATGAGC AAGAAGAAGA GGAAGCTTTC 
AATCAGAAGC ATGCACTGCA AAAAGCGAAG GAGGTCAGCC CCATGTCTGC ACCTAATGCA 
TCATGTATTG AGAAGGAGCG CAGAAAACGG CACACATTGT CTATATGGGA GCAGCGCACA 
AGCCAGCTAA GGAGGCACAT ACAGATGTCT AGTCAAGAAG CTCTCCAGAG TGAAGAACTG 
GCAGCATTGA ATCCACACTC CAGTATCTAT CGCAAGACAA AGGAAGCTGC TGAACAAAAC 
ACAGAAAAAG AAGAAGTGCA TCAAGACGCA AAACTGGCAA TAGACCCAGT TGAAGGACCA 
CCAGAAGGTG AAGGAAGCCC TTCTAATAGA AATCTCAGGG AAAACTGGCG CCAGAAGTCC 
AGGCATGGAA ATTCTGAAAA CACTGAGCCA AATGGAACAA CTCCAGAAGA AGCAATGCGC 
CGCAGACAAA GTCAGAGACG CAGCCGTCAT AGAAGAGTTA GAACTGAAGG TCGTGAGCAG 
TGTAGCAATC CAAACAGTAC CTCAGCAAGC AGAGAAGGTG GTCTTGACAA AGTCTCTGCA 
GAGGATGGAA GAACAATTCC AGAAGAGAAG AGCAATGGAG AAGCCAAAGA AGTCAAAGGG 
GTTGATGATG AGCCTCTAAA GATTTGTATA TCTTCAAGAA ATTGCGAGGA CAGAAATATC 
AGGACTTTGG GTTGTGAGGG TATTGCCTTG AACGATACAC CCCTGCAGGA TGTGAACAGC 
CAAGAGAAGG ACAGTAGCCC TCCTGATTTG GATTGCAGTG AGCAGGCTCT GTTGGGCAGC 
ATTCAGAGCC AGGAAAGCCC TAATCCAACC AGCTGCAGTT TGCCAGATGT GTCCAGCAGC 
ACCGAGAGAG TCACAGAGAG CACCGTCATT ACCTTACCAG ATACTGGAAT GCCCACCGAC 
TGCACCGTGG TACAGATGAG TGAAGGCAGG GATGGAGAAG TCAACTTTGG GTCTTCGCAG 
CAAGAAAAAG AAGAGGAGGA AGCTGAGGAT GGAGAAGAAA GAACTATAAA AAGAAAAGAG 
AAATTGTTGG AGTCCCCCAA AGCCATTGTA CCGTACAGTT CAATGCTCAT CTTCAGCTCC 
ACCAATCCCA TAAGAAAAGT ATGTCATTAC ATTGTTAACC TACGATACTT TGAGATGAGC 
ATCCTGCTGG TAATAGCAGC AAGTAGTATA GCCTTAGCAG CGGAGGACCC TGTACTGACC 
AACTCGGACC GCAACAAAGT TCTTCGCTAT TTTGACTATG TTTTCACAGG GGTCTTCACT 
TTTGAGATGA TTATAAAGAT GATTGACCAA GGTTTGATTT TACACGAGGG CTCCTACTTT 
CGTGACCTAT GGAACATCTT GGATTTTGTT GTTGTTGTGG GAGCCTTAAT TGCGTTTGCC 
TTAGCGAATG TTTTGGGGAC TAACAAAGGC CGGGACATCA AAACCATTAA GTCTCTACGT 
GTGTTACGGG TGCTGCGTCC ACTAAAAACA ATCAAACGCC TGCCAAAACT GAAGGCAGTC 
TTTGATTGTG TGGTTAACTC TTTAAAGAAT GTCTTTAATA TTCTCATCGT ATACAAACTC 
TTCATGTTCA TATTTGCTGT AATCGCTGTC CAGCTGTTTA AGGGCAAGTT CTTCTATTGT 
ACAGACAGCT CAAAGGAGAC AGAGAAAGAG TGCATAGGGA GTTACATCGA TCACGAAAAG 
AACAAGATGG AGGTGAAGCG GAGAGAGTGG AAAAGACATG ATTTCCATTA TGACAACATA 
ATATGGGCTC TTCTTACCCT CTTTACTGTA TCAACAGGGG AAGGCTGGCC TCAGGTTCTG 
CAGCACTCTG TTGATATAAC TGAAGAAGAT AGGGGTCCAA GCCGAAGCAA CCGTATGGAG 
ATGTCCATTT TCTATGTTAT CTACTTTGTG GTCTTCCCCT TCTTCTTTGT AAATATATTT 
GTGGCTCTGA TTATCATCAC ATTCCAGGAG CAAGGAGACA AGATGATGGA GGAATGTTCC 
CTAGAAAAGA ATGAGAGAGC CTGCATAGAC TTTGCAATAA GTGCTAAGCC ACTTACACGA 
TATATGCCAC AGAACAGGCA CACATTCCAG TACCGGGTCT GGCACTTCGT GGTCTCCCCA 
TCATTTGAAT ACACTATCAT GGCTATGATT GCACTAAACA CAGTGGTACT CATGATGAAG 
TATTATTCTG CGCCATACAC ATATGAGCTT GCATTAAAGT ATCTCAATAT TGCATTCACA 
ATGCTATTTT CTCTGGAGTG CATCCTGAAA ATAATTGCAT TTGGGTTTTT GAATTACTTT 
CGGGATACTT GGAACATTTT TGACTTCATT ACTGTAATTG GTAGCATCAC GGAAATTATT 
CTAACAGATA GTAAGTTGGT GAACACCAGC AGCTTTAATA TGAGCTTTCT GAAGCTGTTC 
CGAGCGGCGC GACTTATTAA ACTTCTCCGG CAAGGGTACA CCATCCGGAT CCTGCTGTGG 
ACTTTTGTTC AGTCCTTTAA GGCACTTCCT TATGTGTGTC TTCTCATTGC AATGTTGTTC 
TTCATCTATG CCATTATTGG GATGCAGGTG TTTGGCAATA TCCGCCTGGA AGAGGACAGT 
CATATTAATA GGCACAACAA TTTTAGAAGC TTCTTTAGTT CTCTTATGTT ACTCTTCAGG 
AGTGCCACAG GAGAAGCGTG GCAGGAGATC ATGCTTTCAT GCCTTAGTGA TAAGGGTTGT 
GAGCCAGACA CCAGTGCAGC CTCTGGACAC AACGAGAATG AGCGCTGTGG AACTGAACTT 
GCATATGTTT ATTTTGTTTC ATTCATTTTC TTCTGCTCCT TCCTCATGCT AAATCTGTTT 
GTGGCTGTCA TTATGGACAA CTTTGAGTAC TTGACACGGG ACTCTTCCAT TTTGGGACCT 
CATCACTTGG ATGAGTTTGT TCGGATATGG GCTGAATATG ATCGAGCAGC ATGTGGCAGG 
ATCCCCTATA AGGATATGTA CAAATTAGTC CGAGTCATCT CGCCTCCGCT CGGCCTAGGA 
GAGAACTGCC CATACAGGGT GGCATGCAAG AGATTGGTCC TGATGAATAT GCCAGTGTCG 
GAGGACATGA CGGTTCATTT CACATCAACT CTTATGGCCC TAATACGCAC GGCACTGGAT 
ATCAAAATAG CTAAAGGAGG TGCTGACAGG CAACAGCTGG ACTCAGAGCT GCAGAAAGAT 
ATACTTGCCA TTTGGCCAAA TCTCTCCCAG AAAATGATCG ACTTACTTGT GCCAATGCCA 
AGAGCCTCTG ATCTGACGGT GGGGAAGATT TACGCTGCAA TGATGATCAT GGATTACTAT 
AAACAGAGCA AAGCGAAAAA ACAAAGGCAG CAGCTGGAGG AGCAGAAAAA TGCACCTATG 
TTTCAGAGAA TGGAGGCTTC TGCCCTCCCT GAGGAGATCT TATCCAATGC CAAAGCTTTA 
CCATACCTTC AGCAAGACAC TCTATCAGGC CTGGGTGGAT TTCCTTCACT GAGTCCCTTG 
TCCCCAGAAG AAGTATTCCA GCCGCCATGC CTAGACGGGG AAGAGGAGGA AGACTTTGAA 
GAGCAGCTGT CTCTGGAGCC TGAGGTTAGA GAGTTTAGAA GGGAGCAGCT CCCTAACCGA 
GGCAGCTACA TCCCTGTGGA GATGCTGGAA CGTCCAGCGT CTGTGAGAGC TTCTTCCATG 
CCACGTCTGG CTGAGAATCC TCAGGTCCTG TCAGACGTAA GCTCCATGCG TCGCTCCTTC 
TCCACAACTG CAGAGAAGCG AGTGCGCAGC TCGTGGCTAG ATGAGTTCAC TATGGACAGG 
AGTAGCACTG ACTGTGCATA CAGATACCAG AGGCGCAGTT ATCATGCATC CTTAAGACTG 
TCTGCACATC GCCTGAATAC AGATACAGGT CACTGGTCAG ATAATCACCG TTCTGGCGGA 
AGGGAGCGAG GAAGGTCCAA AGAGAGAAAA CACTTGCTGT CACCCGATCC TTCCCGCTGT 
AATTCTGAGG AAAGGAGCCC ACAAGCTGCC TGTGAACACC GAGATCACCA ACTGTCCCCT 
GGGCATACTC CCAGTAGTGT ACAGATTCTT GACTCTCTGA GTGAAAGTTG TACCCCTTCA 
GACACCAGCA CCCCAAGAAG AGGCCGTAGA CAGCTTCCTT CTGTGCCTCC AACTCCTAGA 
CCCCTTATCT CTTTCTCCTC GTTAATCCGC CGGCAGAAAC ACAGCCCCCC TCCTCAAAAT 
AAAAATGATA CTATCCTTCC TAGTGAGCCT GGTCTGACTG AGTCTTCCTG CTCCCTTGGG 
GGTCATCAGA GCTCCCAGTC CACACCACAG CATTATATCT CAGAGCCCTA CCTGGCACTG 
CATGAGGAGA TGCAAAGCTC AGACTGCCCA GCAGAGGACA CACTCACCTT TGAGGTTGCT 
GTGGCTACTA GTCTGGGCCG CTCGAACACC ATTGGCTCTG CCCCTCTACG TCATGGCTGG 
CAAATGCCCA ATGGTCACTA CCGGCGTCGA AGGAAGGGAG GAATTCCACC AGTGATACCC 
TCCAGAGAGT TAAGTGACAC AGAAGAGGAT GATCGATGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1e ( XM_031900980.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756840.1 Xenopus tropicalis calcium channel, voltage-dependent, R type, alpha 1E subunit (cacna1e), transcript variant X3, mRNA.

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CloneID	OXa57231
Clone ID Related Accession (Same CDS sequence)	XM_031900980.1
Accession Version	XM_031900980.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7170bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTATTAA AGATGCTGGG ATTAAGCTCC TGTTCAACCA GTGCTCATAC ACTCAATGAG 
CCTGACCAGT CAAGCACACT AGCCATACCA GCATTGGATG CCGACACTCA GAGATATGTC 
TTGGCCCTTC CAGAAGCAGA GACATGGCGC AAGCATCGAT TGGGCCTGAC ACAGGCTACT 
CACTCTTGCA ATCTTCTAGA ACCTGAGACA CTTTCTGAAA CCCTAGTTTC ACGCACTGCA 
AGTTACGATG CCTTGTATGA CCTGCGAGCA AGAGAAGCGG AGTCTAACTT CCCATTTGAA 
GGCAGCAGCA TGTTTGAGGT GACGTCTGGG ATGGATTCTG AGTTTCCTTT AAGCCCCCAT 
GTTATAAAGC GAAGGAGAGG AGGCCTGATA GAACAAAAGG ATATCATCAA GGCCCACGAA 
GCTCATAAGA TTCAGAGTAC TCCCCAGGCT CGACGGAAGG AATGGGAGAT GGCTCGTTTC 
GGAGAGGCTG TGACTGGCAG GTTGAGCTCC GCAGATGGGG GCTCAGAGAG GAGCAAGACC 
CTACAAGGTT CCCCAGCTGC ACCTGTTGGG ACCCAAGCAG CTTTCAAGCA ATCCAAAGCC 
CAGAGGGCAA GGACCATGGC TTTATACAAC CCCATCCCTG TCCGACAGAA CTGCTTCACC 
GTGAACAGGT CTCTCTTCAT TTTCGGAGAA GATAATATTA TCAGGAAATA CGCCAAGAAG 
CTCATTGATT GGCCTCCTTT TGAATACATG ATCCTTGCCA CAATTATTGC AAACTGCATT 
GTCCTGGCTT TGGAGCAGCA TCTTCCAGGC GATGACAAGA CTCCAATGTC TCGCAGATTG 
GAAAAGACTG AGCCCTATTT CATTGGGATA TTTTGCTTTG AAGCTGGAAT TAAAATTGTC 
GCTCTGGGAC TGGTTTTTCA CAAAGGTTCT TATCTTCGGA ATGGCTGGAA TGTCATGGAC 
TTTATAGTAG TGCTCAGTGG CATCCTGGCC ACAGCTGGAA CTCACTTCAA TACTCACGTG 
GACCTGCGTA CTTTGCGGGC AGTGCGTGTT CTCAGGCCGT TGAAGCTGGT CTCGGGGATT 
CCCAGTTTAC AGATTGTGTT GAAGTCAATC ATGAAGGCGA TGGTCCCTCT GCTACAGATT 
GGATTGTTGC TCTTCTTTGC CATCCTTATG TTTGCTATCA TCGGCCTTGA GTTTTACAGT 
GGAAAGCTAC ACAAGGCCTG TTACAGGAAC AACTCTGGAA TGGAGCTAGA CCCCATGGAT 
CCACCTCACC CTTGTGGAGT GCCCCGCTGC CCAGCTGGGT ATGAGTGCAA GGAATGGATC 
GGACCCAATG ATGGAATAAC ACAGTTTGAC AACATCCTAT TTGCGGTGCT GACAGTCTTC 
CAGTGTATAA CTATGGAGGG CTGGACCACA GTGCTGTACA ATACCAATGA TGCCTTAGGA 
GCCACATGGA ACTGGCTGTA CTTCATTCCC CTCATTATCA TCGGATCCTT CTTTGTCCTT 
AATCTTGTCC TTGGAGTGCT GTCCGGTGAA TTTGCCAAAG AGAGGGAAAG AGTGGAAAAC 
CGCCGGGCTT TCATGAAGTT GCGCAGGCAG CAGCAGATTG AGAGGGAGTT GAATGGCTAT 
CGGGCATGGA TAGATAAAGC TGAGGAAGTC ATGTTGGCAG AAGAAAACAA AAATTCTGGG 
ACCTCTGCTT TAGAAGTTCT TAGGCGAGCA ACCATTAAAC GAAGTAGAAC AGAAGCCATG 
AACCGGGACT CTACTGAGGA CCATTGTGTT GACATATCCT CTGTTGGCAC CCCCCTTGCT 
AGAGCAAGCA TCAAAAGTAC CAAAATGGAT GGGGCATCAT ATTTTCGGCA CAAGGAAAAA 
CTATTGCGTG TCTCCGTTCG TCACATGATC AAATCCCAGG TTTTCTACTG GATGGTCCTG 
GGAGTGGTAG CACTTAACAC TTTCTGTGTG GCTATTGTCC ATCACAACCA GACCCTGTGG 
CTCTCAAATG TTCTTTACTA TGCAGAATTT CTGTTTCTTG GGTTATTTTT GGTGGAGATG 
GCTTTTAAAA TGTATGGAAT GGGTCCACGT CTTTACTTTC ACTCATCCTT CAACTGCTTT 
GATTGCGGGG TCACAATAGG TAGTATATTT GAAGTACTTT GGGCCATATT TAGACCAGGA 
ACTTCCTTTG GAATCAGTGT CTTAAGAGCT CTCCGACTTT TGAGAATCTT CAAAATAACA 
AAATACTGGG CCTCACTACG AAACCTTGTA GTATCATTGA TGAATTCTAT GAAATCCATC 
ATCAGCCTGC TCTTCCTTCT ATTCCTCTTC ATAGTTGTTT TTGCTCTCCT TGGAATGCAG 
TTATATGGTG GCAGATTCAA TTTTAATGAT GGCACTCCTT CTGCAAACTT TGATACATTC 
CCTGCAGCCA TCATGACTGT ATTCCAGATT CTGACAGGAG AGGATTGGAA TGAAGTGATG 
TACAATGGTA TTCGCTCCCA AGGTGGTGTT CGATCCGGAA TGTGGTCTTC CATATACTTT 
ATTGTCCTTA CCCTTTTTGG AAATTACACA CTGCTAAATG TATTTCTTGC CATTGCTGTG 
GACAACCTGG CTAATGCACA GGAGTTAACT AAGGATGAGC AAGAAGAAGA GGAAGCTTTC 
AATCAGAAGC ATGCACTGCA AAAAGCGAAG GAGGTCAGCC CCATGTCTGC ACCTAATGCA 
TCATGTATTG AGAAGGAGCG CAGAAAACGG CACACATTGT CTATATGGGA GCAGCGCACA 
AGCCAGCTAA GGAGGCACAT ACAGATGTCT AGTCAAGAAG CTCTCCAGAG TGAAGAACTG 
GCAGCATTGA ATCCACACTC CAGTATCTAT CGCAAGACAA AGGAAGCTGC TGAACAAAAC 
ACAGAAAAAG AAGAAGTGCA TCAAGACGCA AAACTGGCAA TAGACCCAGT TGAAGGACCA 
CCAGAAGGTG AAGGAAGCCC TTCTAATAGA AATCTCAGGG AAAACTGGCG CCAGAAGTCC 
AGGCATGGAA ATTCTGAAAA CACTGAGCCA AATGGAACAA CTCCAGAAGA AGCAATGCGC 
CGCAGACAAA GTCAGAGACG CAGCCGTCAT AGAAGAGTTA GAACTGAAGG TCGTGAGCAG 
TGTAGCAATC CAAACAGTAC CTCAGCAAGC AGAGAAGGTG GTCTTGACAA AGTCTCTGCA 
GAGGATGGAA GAACAATTCC AGAAGAGAAG AGCAATGGAG AAGCCAAAGA AGTCAAAGGG 
GTTGATGATG AGCCTCTAAA GATTTGTATA TCTTCAAGAA ATTGCGAGGA CAGAAATATC 
AGGACTTTGG GTTGTGAGGG TATTGCCTTG AACGATACAC CCCTGCAGGA TGTGAACAGC 
CAAGAGAAGG ACAGTAGCCC TCCTGATTTG GATTGCAGTG AGCAGGCTCT GTTGGGCAGC 
ATTCAGAGCC AGGAAAGCCC TAATCCAACC AGCTGCAGTT TGCCAGATGT GTCCAGCAGC 
ACCGAGAGAG TCACAGAGAG CACCGTCATT ACCTTACCAG ATACTGGAAT GCCCACCGAC 
TGCACCGTGG TACAGATGAG TGAAGGCAGG GATGGAGAAG TCAACTTTGG GTCTTCGCAG 
CAAGAAAAAG AAGAGGAGGA AGCTGAGGAT GGAGAAGAAA GAACTATAAA AAGAAAAGAG 
AAATTGTTGG AGTCCCCCAA AGCCATTGTA CCGTACAGTT CAATGCTCAT CTTCAGCTCC 
ACCAATCCCA TAAGAAAAGT ATGTCATTAC ATTGTTAACC TACGATACTT TGAGATGAGC 
ATCCTGCTGG TAATAGCAGC AAGTAGTATA GCCTTAGCAG CGGAGGACCC TGTACTGACC 
AACTCGGACC GCAACAAAGT TCTTCGCTAT TTTGACTATG TTTTCACAGG GGTCTTCACT 
TTTGAGATGA TTATAAAGAT GATTGACCAA GGTTTGATTT TACACGAGGG CTCCTACTTT 
CGTGACCTAT GGAACATCTT GGATTTTGTT GTTGTTGTGG GAGCCTTAAT TGCGTTTGCC 
TTAGCGACTA ACAAAGGCCG GGACATCAAA ACCATTAAGT CTCTACGTGT GTTACGGGTG 
CTGCGTCCAC TAAAAACAAT CAAACGCCTG CCAAAACTGA AGGCAGTCTT TGATTGTGTG 
GTTAACTCTT TAAAGAATGT CTTTAATATT CTCATCGTAT ACAAACTCTT CATGTTCATA 
TTTGCTGTAA TCGCTGTCCA GCTGTTTAAG GGCAAGTTCT TCTATTGTAC AGACAGCTCA 
AAGGAGACAG AGAAAGAGTG CATAGGGAGT TACATCGATC ACGAAAAGAA CAAGATGGAG 
GTGAAGCGGA GAGAGTGGAA AAGACATGAT TTCCATTATG ACAACATAAT ATGGGCTCTT 
CTTACCCTCT TTACTGTATC AACAGGGGAA GGCTGGCCTC AGGTTCTGCA GCACTCTGTT 
GATATAACTG AAGAAGATAG GGGTCCAAGC CGAAGCAACC GTATGGAGAT GTCCATTTTC 
TATGTTATCT ACTTTGTGGT CTTCCCCTTC TTCTTTGTAA ATATATTTGT GGCTCTGATT 
ATCATCACAT TCCAGGAGCA AGGAGACAAG ATGATGGAGG AATGTTCCCT AGAAAAGAAT 
GAGAGAGCCT GCATAGACTT TGCAATAAGT GCTAAGCCAC TTACACGATA TATGCCACAG 
AACAGGCACA CATTCCAGTA CCGGGTCTGG CACTTCGTGG TCTCCCCATC ATTTGAATAC 
ACTATCATGG CTATGATTGC ACTAAACACA GTGGTACTCA TGATGAAGTA TTATTCTGCG 
CCATACACAT ATGAGCTTGC ATTAAAGTAT CTCAATATTG CATTCACAAT GCTATTTTCT 
CTGGAGTGCA TCCTGAAAAT AATTGCATTT GGGTTTTTGA ATTACTTTCG GGATACTTGG 
AACATTTTTG ACTTCATTAC TGTAATTGGT AGCATCACGG AAATTATTCT AACAGATAGT 
AAGTTGGTGA ACACCAGCAG CTTTAATATG AGCTTTCTGA AGCTGTTCCG AGCGGCGCGA 
CTTATTAAAC TTCTCCGGCA AGGGTACACC ATCCGGATCC TGCTGTGGAC TTTTGTTCAG 
TCCTTTAAGG CACTTCCTTA TGTGTGTCTT CTCATTGCAA TGTTGTTCTT CATCTATGCC 
ATTATTGGGA TGCAGGTGTT TGGCAATATC CGCCTGGAAG AGGACAGTCA TATTAATAGG 
CACAACAATT TTAGAAGCTT CTTTAGTTCT CTTATGTTAC TCTTCAGGAG TGCCACAGGA 
GAAGCGTGGC AGGAGATCAT GCTTTCATGC CTTAGTGATA AGGGTTGTGA GCCAGACACC 
AGTGCAGCCT CTGGACACAA CGAGAATGAG CGCTGTGGAA CTGAACTTGC ATATGTTTAT 
TTTGTTTCAT TCATTTTCTT CTGCTCCTTC CTCATGCTAA ATCTGTTTGT GGCTGTCATT 
ATGGACAACT TTGAGTACTT GACACGGGAC TCTTCCATTT TGGGACCTCA TCACTTGGAT 
GAGTTTGTTC GGATATGGGC TGAATATGAT CGAGCAGCAT GTGGCCGTAT CCATTACACA 
GAGATGTATG ACATGCTGAC TCTCATGTCA CCACCTCTAG GCCTTGGAAA GAGATGCCCT 
AACAAAGTGG CTTATAAGAG ATTGGTCCTG ATGAATATGC CAGTGTCGGA GGACATGACG 
GTTCATTTCA CATCAACTCT TATGGCCCTA ATACGCACGG CACTGGATAT CAAAATAGCT 
AAAGGAGGTG CTGACAGGCA ACAGCTGGAC TCAGAGCTGC AGAAAGATAT ACTTGCCATT 
TGGCCAAATC TCTCCCAGAA AATGATCGAC TTACTTGTGC CAATGCCAAG AGCCTCTGAT 
CTGACGGTGG GGAAGATTTA CGCTGCAATG ATGATCATGG ATTACTATAA ACAGAGCAAA 
GCGAAAAAAC AAAGGCAGCA GCTGGAGGAG CAGAAAAATG CACCTATGTT TCAGAGAATG 
GAGGCTTCTG CCCTCCCTGA GGAGATCTTA TCCAATGCCA AAGCTTTACC ATACCTTCAG 
CAAGACACTC TATCAGGCCT GGGTGGATTT CCTTCACTGA GTCCCTTGTC CCCAGAAGAA 
GTATTCCAGC CGCCATGCCT AGACGGGGAA GAGGAGGAAG ACTTTGAAGA GCAGCTGTCT 
CTGGAGCCTG AGGTTAGAGA GTTTAGAAGG GAGCAGCTCC CTAACCGAGG CAGCTACATC 
CCTGTGGAGA TGCTGGAACG TCCAGCGTCT GTGAGAGCTT CTTCCATGCC ACGTCTGGCT 
GAGAATCCTC AGGTCCTGTC AGACGTAAGC TCCATGCGTC GCTCCTTCTC CACAACTGCA 
GAGAAGCGAG TGCGCAGCTC GTGGCTAGAT GAGTTCACTA TGGACAGGAG TAGCACTGAC 
TGTGCATACA GATACCAGAG GCGCAGTTAT CATGCATCCT TAAGACTGTC TGCACATCGC 
CTGAATACAG ATACAGGTCA CTGGTCAGAT AATCACCGTT CTGGCGGAAG GGAGCGAGGA 
AGGTCCAAAG AGAGAAAACA CTTGCTGTCA CCCGATCCTT CCCGCTGTAA TTCTGAGGAA 
AGGAGCCCAC AAGCTGCCTG TGAACACCGA GATCACCAAC TGTCCCCTGG GCATACTCCC 
AGTAGTGTAC AGATTCTTGA CTCTCTGAGT GAAAGTTGTA CCCCTTCAGA CACCAGCACC 
CCAAGAAGAG GCCGTAGACA GCTTCCTTCT GTGCCTCCAA CTCCTAGACC CCTTATCTCT 
TTCTCCTCGT TAATCCGCCG GCAGAAACAC AGCCCCCCTC CTCAAAATAA AAATGATACT 
ATCCTTCCTA GTGAGCCTGG TCTGACTGAG TCTTCCTGCT CCCTTGGGGG TCATCAGAGC 
TCCCAGTCCA CACCACAGCA TTATATCTCA GAGCCCTACC TGGCACTGCA TGAGGAGATG 
CAAAGCTCAG ACTGCCCAGC AGAGGACACA CTCACCTTTG AGGTTGCTGT GGCTACTAGT 
CTGGGCCGCT CGAACACCAT TGGCTCTGCC CCTCTACGTC ATGGCTGGCA AATGCCCAAT 
GGTCACTACC GGCGTCGAAG GAAGGGAGGA ATTCCACCAG TGATACCCTC CAGAGAGTTA 
AGTGACACAG AAGAGGATGA TCGATGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756840.1
CDS	285..7454
Translation	MVLKMLGLSSCSTSAHTLNEPDQSSTLAIPALDADTQRYVLALPEAETWRKHRLGLTQATHSCNLLEPETLSETLVSRTASYDALYDLRAREAESNFPFEGSSMFEVTSGMDSEFPLSPHVIKRRRGGLIEQKDIIKAHEAHKIQSTPQARRKEWEMARFGEAVTGRLSSADGGSERSKTLQGSPAAPVGTQAAFKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIIRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPGDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGLVFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHKACYRNNSGMELDPMDPPHPCGVPRCPAGYECKEWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNSGTSALEVLRRATIKRSRTEAMNRDSTEDHCVDISSVGTPLARASIKSTKMDGASYFRHKEKLLRVSVRHMIKSQVFYWMVLGVVALNTFCVAIVHHNQTLWLSNVLYYAEFLFLGLFLVEMAFKMYGMGPRLYFHSSFNCFDCGVTIGSIFEVLWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMNSMKSIISLLFLLFLFIVVFALLGMQLYGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVRSGMWSSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNASCIEKERRKRHTLSIWEQRTSQLRRHIQMSSQEALQSEELAALNPHSSIYRKTKEAAEQNTEKEEVHQDAKLAIDPVEGPPEGEGSPSNRNLRENWRQKSRHGNSENTEPNGTTPEEAMRRRQSQRRSRHRRVRTEGREQCSNPNSTSASREGGLDKVSAEDGRTIPEEKSNGEAKEVKGVDDEPLKICISSRNCEDRNIRTLGCEGIALNDTPLQDVNSQEKDSSPPDLDCSEQALLGSIQSQESPNPTSCSLPDVSSSTERVTESTVITLPDTGMPTDCTVVQMSEGRDGEVNFGSSQQEKEEEEAEDGEERTIKRKEKLLESPKAIVPYSSMLIFSSTNPIRKVCHYIVNLRYFEMSILLVIAASSIALAAEDPVLTNSDRNKVLRYFDYVFTGVFTFEMIIKMIDQGLILHEGSYFRDLWNILDFVVVVGALIAFALATNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKETEKECIGSYIDHEKNKMEVKRREWKRHDFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDITEEDRGPSRSNRMEMSIFYVIYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPYTYELALKYLNIAFTMLFSLECILKIIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSSFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIRLEEDSHINRHNNFRSFFSSLMLLFRSATGEAWQEIMLSCLSDKGCEPDTSAASGHNENERCGTELAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRIWAEYDRAACGRIHYTEMYDMLTLMSPPLGLGKRCPNKVAYKRLVLMNMPVSEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKDILAIWPNLSQKMIDLLVPMPRASDLTVGKIYAAMMIMDYYKQSKAKKQRQQLEEQKNAPMFQRMEASALPEEILSNAKALPYLQQDTLSGLGGFPSLSPLSPEEVFQPPCLDGEEEEDFEEQLSLEPEVREFRREQLPNRGSYIPVEMLERPASVRASSMPRLAENPQVLSDVSSMRRSFSTTAEKRVRSSWLDEFTMDRSSTDCAYRYQRRSYHASLRLSAHRLNTDTGHWSDNHRSGGRERGRSKERKHLLSPDPSRCNSEERSPQAACEHRDHQLSPGHTPSSVQILDSLSESCTPSDTSTPRRGRRQLPSVPPTPRPLISFSSLIRRQKHSPPPQNKNDTILPSEPGLTESSCSLGGHQSSQSTPQHYISEPYLALHEEMQSSDCPAEDTLTFEVAVATSLGRSNTIGSAPLRHGWQMPNGHYRRRRKGGIPPVIPSRELSDTEEDDRC

Target ORF information:

RefSeq Version	XM_031900980.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calcium channel, voltage-dependent, R type, alpha 1E subunit (cacna1e), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031900980.1

ORF Insert Sequence:

ATGGTATTAA AGATGCTGGG ATTAAGCTCC TGTTCAACCA GTGCTCATAC ACTCAATGAG 
CCTGACCAGT CAAGCACACT AGCCATACCA GCATTGGATG CCGACACTCA GAGATATGTC 
TTGGCCCTTC CAGAAGCAGA GACATGGCGC AAGCATCGAT TGGGCCTGAC ACAGGCTACT 
CACTCTTGCA ATCTTCTAGA ACCTGAGACA CTTTCTGAAA CCCTAGTTTC ACGCACTGCA 
AGTTACGATG CCTTGTATGA CCTGCGAGCA AGAGAAGCGG AGTCTAACTT CCCATTTGAA 
GGCAGCAGCA TGTTTGAGGT GACGTCTGGG ATGGATTCTG AGTTTCCTTT AAGCCCCCAT 
GTTATAAAGC GAAGGAGAGG AGGCCTGATA GAACAAAAGG ATATCATCAA GGCCCACGAA 
GCTCATAAGA TTCAGAGTAC TCCCCAGGCT CGACGGAAGG AATGGGAGAT GGCTCGTTTC 
GGAGAGGCTG TGACTGGCAG GTTGAGCTCC GCAGATGGGG GCTCAGAGAG GAGCAAGACC 
CTACAAGGTT CCCCAGCTGC ACCTGTTGGG ACCCAAGCAG CTTTCAAGCA ATCCAAAGCC 
CAGAGGGCAA GGACCATGGC TTTATACAAC CCCATCCCTG TCCGACAGAA CTGCTTCACC 
GTGAACAGGT CTCTCTTCAT TTTCGGAGAA GATAATATTA TCAGGAAATA CGCCAAGAAG 
CTCATTGATT GGCCTCCTTT TGAATACATG ATCCTTGCCA CAATTATTGC AAACTGCATT 
GTCCTGGCTT TGGAGCAGCA TCTTCCAGGC GATGACAAGA CTCCAATGTC TCGCAGATTG 
GAAAAGACTG AGCCCTATTT CATTGGGATA TTTTGCTTTG AAGCTGGAAT TAAAATTGTC 
GCTCTGGGAC TGGTTTTTCA CAAAGGTTCT TATCTTCGGA ATGGCTGGAA TGTCATGGAC 
TTTATAGTAG TGCTCAGTGG CATCCTGGCC ACAGCTGGAA CTCACTTCAA TACTCACGTG 
GACCTGCGTA CTTTGCGGGC AGTGCGTGTT CTCAGGCCGT TGAAGCTGGT CTCGGGGATT 
CCCAGTTTAC AGATTGTGTT GAAGTCAATC ATGAAGGCGA TGGTCCCTCT GCTACAGATT 
GGATTGTTGC TCTTCTTTGC CATCCTTATG TTTGCTATCA TCGGCCTTGA GTTTTACAGT 
GGAAAGCTAC ACAAGGCCTG TTACAGGAAC AACTCTGGAA TGGAGCTAGA CCCCATGGAT 
CCACCTCACC CTTGTGGAGT GCCCCGCTGC CCAGCTGGGT ATGAGTGCAA GGAATGGATC 
GGACCCAATG ATGGAATAAC ACAGTTTGAC AACATCCTAT TTGCGGTGCT GACAGTCTTC 
CAGTGTATAA CTATGGAGGG CTGGACCACA GTGCTGTACA ATACCAATGA TGCCTTAGGA 
GCCACATGGA ACTGGCTGTA CTTCATTCCC CTCATTATCA TCGGATCCTT CTTTGTCCTT 
AATCTTGTCC TTGGAGTGCT GTCCGGTGAA TTTGCCAAAG AGAGGGAAAG AGTGGAAAAC 
CGCCGGGCTT TCATGAAGTT GCGCAGGCAG CAGCAGATTG AGAGGGAGTT GAATGGCTAT 
CGGGCATGGA TAGATAAAGC TGAGGAAGTC ATGTTGGCAG AAGAAAACAA AAATTCTGGG 
ACCTCTGCTT TAGAAGTTCT TAGGCGAGCA ACCATTAAAC GAAGTAGAAC AGAAGCCATG 
AACCGGGACT CTACTGAGGA CCATTGTGTT GACATATCCT CTGTTGGCAC CCCCCTTGCT 
AGAGCAAGCA TCAAAAGTAC CAAAATGGAT GGGGCATCAT ATTTTCGGCA CAAGGAAAAA 
CTATTGCGTG TCTCCGTTCG TCACATGATC AAATCCCAGG TTTTCTACTG GATGGTCCTG 
GGAGTGGTAG CACTTAACAC TTTCTGTGTG GCTATTGTCC ATCACAACCA GACCCTGTGG 
CTCTCAAATG TTCTTTACTA TGCAGAATTT CTGTTTCTTG GGTTATTTTT GGTGGAGATG 
GCTTTTAAAA TGTATGGAAT GGGTCCACGT CTTTACTTTC ACTCATCCTT CAACTGCTTT 
GATTGCGGGG TCACAATAGG TAGTATATTT GAAGTACTTT GGGCCATATT TAGACCAGGA 
ACTTCCTTTG GAATCAGTGT CTTAAGAGCT CTCCGACTTT TGAGAATCTT CAAAATAACA 
AAATACTGGG CCTCACTACG AAACCTTGTA GTATCATTGA TGAATTCTAT GAAATCCATC 
ATCAGCCTGC TCTTCCTTCT ATTCCTCTTC ATAGTTGTTT TTGCTCTCCT TGGAATGCAG 
TTATATGGTG GCAGATTCAA TTTTAATGAT GGCACTCCTT CTGCAAACTT TGATACATTC 
CCTGCAGCCA TCATGACTGT ATTCCAGATT CTGACAGGAG AGGATTGGAA TGAAGTGATG 
TACAATGGTA TTCGCTCCCA AGGTGGTGTT CGATCCGGAA TGTGGTCTTC CATATACTTT 
ATTGTCCTTA CCCTTTTTGG AAATTACACA CTGCTAAATG TATTTCTTGC CATTGCTGTG 
GACAACCTGG CTAATGCACA GGAGTTAACT AAGGATGAGC AAGAAGAAGA GGAAGCTTTC 
AATCAGAAGC ATGCACTGCA AAAAGCGAAG GAGGTCAGCC CCATGTCTGC ACCTAATGCA 
TCATGTATTG AGAAGGAGCG CAGAAAACGG CACACATTGT CTATATGGGA GCAGCGCACA 
AGCCAGCTAA GGAGGCACAT ACAGATGTCT AGTCAAGAAG CTCTCCAGAG TGAAGAACTG 
GCAGCATTGA ATCCACACTC CAGTATCTAT CGCAAGACAA AGGAAGCTGC TGAACAAAAC 
ACAGAAAAAG AAGAAGTGCA TCAAGACGCA AAACTGGCAA TAGACCCAGT TGAAGGACCA 
CCAGAAGGTG AAGGAAGCCC TTCTAATAGA AATCTCAGGG AAAACTGGCG CCAGAAGTCC 
AGGCATGGAA ATTCTGAAAA CACTGAGCCA AATGGAACAA CTCCAGAAGA AGCAATGCGC 
CGCAGACAAA GTCAGAGACG CAGCCGTCAT AGAAGAGTTA GAACTGAAGG TCGTGAGCAG 
TGTAGCAATC CAAACAGTAC CTCAGCAAGC AGAGAAGGTG GTCTTGACAA AGTCTCTGCA 
GAGGATGGAA GAACAATTCC AGAAGAGAAG AGCAATGGAG AAGCCAAAGA AGTCAAAGGG 
GTTGATGATG AGCCTCTAAA GATTTGTATA TCTTCAAGAA ATTGCGAGGA CAGAAATATC 
AGGACTTTGG GTTGTGAGGG TATTGCCTTG AACGATACAC CCCTGCAGGA TGTGAACAGC 
CAAGAGAAGG ACAGTAGCCC TCCTGATTTG GATTGCAGTG AGCAGGCTCT GTTGGGCAGC 
ATTCAGAGCC AGGAAAGCCC TAATCCAACC AGCTGCAGTT TGCCAGATGT GTCCAGCAGC 
ACCGAGAGAG TCACAGAGAG CACCGTCATT ACCTTACCAG ATACTGGAAT GCCCACCGAC 
TGCACCGTGG TACAGATGAG TGAAGGCAGG GATGGAGAAG TCAACTTTGG GTCTTCGCAG 
CAAGAAAAAG AAGAGGAGGA AGCTGAGGAT GGAGAAGAAA GAACTATAAA AAGAAAAGAG 
AAATTGTTGG AGTCCCCCAA AGCCATTGTA CCGTACAGTT CAATGCTCAT CTTCAGCTCC 
ACCAATCCCA TAAGAAAAGT ATGTCATTAC ATTGTTAACC TACGATACTT TGAGATGAGC 
ATCCTGCTGG TAATAGCAGC AAGTAGTATA GCCTTAGCAG CGGAGGACCC TGTACTGACC 
AACTCGGACC GCAACAAAGT TCTTCGCTAT TTTGACTATG TTTTCACAGG GGTCTTCACT 
TTTGAGATGA TTATAAAGAT GATTGACCAA GGTTTGATTT TACACGAGGG CTCCTACTTT 
CGTGACCTAT GGAACATCTT GGATTTTGTT GTTGTTGTGG GAGCCTTAAT TGCGTTTGCC 
TTAGCGACTA ACAAAGGCCG GGACATCAAA ACCATTAAGT CTCTACGTGT GTTACGGGTG 
CTGCGTCCAC TAAAAACAAT CAAACGCCTG CCAAAACTGA AGGCAGTCTT TGATTGTGTG 
GTTAACTCTT TAAAGAATGT CTTTAATATT CTCATCGTAT ACAAACTCTT CATGTTCATA 
TTTGCTGTAA TCGCTGTCCA GCTGTTTAAG GGCAAGTTCT TCTATTGTAC AGACAGCTCA 
AAGGAGACAG AGAAAGAGTG CATAGGGAGT TACATCGATC ACGAAAAGAA CAAGATGGAG 
GTGAAGCGGA GAGAGTGGAA AAGACATGAT TTCCATTATG ACAACATAAT ATGGGCTCTT 
CTTACCCTCT TTACTGTATC AACAGGGGAA GGCTGGCCTC AGGTTCTGCA GCACTCTGTT 
GATATAACTG AAGAAGATAG GGGTCCAAGC CGAAGCAACC GTATGGAGAT GTCCATTTTC 
TATGTTATCT ACTTTGTGGT CTTCCCCTTC TTCTTTGTAA ATATATTTGT GGCTCTGATT 
ATCATCACAT TCCAGGAGCA AGGAGACAAG ATGATGGAGG AATGTTCCCT AGAAAAGAAT 
GAGAGAGCCT GCATAGACTT TGCAATAAGT GCTAAGCCAC TTACACGATA TATGCCACAG 
AACAGGCACA CATTCCAGTA CCGGGTCTGG CACTTCGTGG TCTCCCCATC ATTTGAATAC 
ACTATCATGG CTATGATTGC ACTAAACACA GTGGTACTCA TGATGAAGTA TTATTCTGCG 
CCATACACAT ATGAGCTTGC ATTAAAGTAT CTCAATATTG CATTCACAAT GCTATTTTCT 
CTGGAGTGCA TCCTGAAAAT AATTGCATTT GGGTTTTTGA ATTACTTTCG GGATACTTGG 
AACATTTTTG ACTTCATTAC TGTAATTGGT AGCATCACGG AAATTATTCT AACAGATAGT 
AAGTTGGTGA ACACCAGCAG CTTTAATATG AGCTTTCTGA AGCTGTTCCG AGCGGCGCGA 
CTTATTAAAC TTCTCCGGCA AGGGTACACC ATCCGGATCC TGCTGTGGAC TTTTGTTCAG 
TCCTTTAAGG CACTTCCTTA TGTGTGTCTT CTCATTGCAA TGTTGTTCTT CATCTATGCC 
ATTATTGGGA TGCAGGTGTT TGGCAATATC CGCCTGGAAG AGGACAGTCA TATTAATAGG 
CACAACAATT TTAGAAGCTT CTTTAGTTCT CTTATGTTAC TCTTCAGGAG TGCCACAGGA 
GAAGCGTGGC AGGAGATCAT GCTTTCATGC CTTAGTGATA AGGGTTGTGA GCCAGACACC 
AGTGCAGCCT CTGGACACAA CGAGAATGAG CGCTGTGGAA CTGAACTTGC ATATGTTTAT 
TTTGTTTCAT TCATTTTCTT CTGCTCCTTC CTCATGCTAA ATCTGTTTGT GGCTGTCATT 
ATGGACAACT TTGAGTACTT GACACGGGAC TCTTCCATTT TGGGACCTCA TCACTTGGAT 
GAGTTTGTTC GGATATGGGC TGAATATGAT CGAGCAGCAT GTGGCCGTAT CCATTACACA 
GAGATGTATG ACATGCTGAC TCTCATGTCA CCACCTCTAG GCCTTGGAAA GAGATGCCCT 
AACAAAGTGG CTTATAAGAG ATTGGTCCTG ATGAATATGC CAGTGTCGGA GGACATGACG 
GTTCATTTCA CATCAACTCT TATGGCCCTA ATACGCACGG CACTGGATAT CAAAATAGCT 
AAAGGAGGTG CTGACAGGCA ACAGCTGGAC TCAGAGCTGC AGAAAGATAT ACTTGCCATT 
TGGCCAAATC TCTCCCAGAA AATGATCGAC TTACTTGTGC CAATGCCAAG AGCCTCTGAT 
CTGACGGTGG GGAAGATTTA CGCTGCAATG ATGATCATGG ATTACTATAA ACAGAGCAAA 
GCGAAAAAAC AAAGGCAGCA GCTGGAGGAG CAGAAAAATG CACCTATGTT TCAGAGAATG 
GAGGCTTCTG CCCTCCCTGA GGAGATCTTA TCCAATGCCA AAGCTTTACC ATACCTTCAG 
CAAGACACTC TATCAGGCCT GGGTGGATTT CCTTCACTGA GTCCCTTGTC CCCAGAAGAA 
GTATTCCAGC CGCCATGCCT AGACGGGGAA GAGGAGGAAG ACTTTGAAGA GCAGCTGTCT 
CTGGAGCCTG AGGTTAGAGA GTTTAGAAGG GAGCAGCTCC CTAACCGAGG CAGCTACATC 
CCTGTGGAGA TGCTGGAACG TCCAGCGTCT GTGAGAGCTT CTTCCATGCC ACGTCTGGCT 
GAGAATCCTC AGGTCCTGTC AGACGTAAGC TCCATGCGTC GCTCCTTCTC CACAACTGCA 
GAGAAGCGAG TGCGCAGCTC GTGGCTAGAT GAGTTCACTA TGGACAGGAG TAGCACTGAC 
TGTGCATACA GATACCAGAG GCGCAGTTAT CATGCATCCT TAAGACTGTC TGCACATCGC 
CTGAATACAG ATACAGGTCA CTGGTCAGAT AATCACCGTT CTGGCGGAAG GGAGCGAGGA 
AGGTCCAAAG AGAGAAAACA CTTGCTGTCA CCCGATCCTT CCCGCTGTAA TTCTGAGGAA 
AGGAGCCCAC AAGCTGCCTG TGAACACCGA GATCACCAAC TGTCCCCTGG GCATACTCCC 
AGTAGTGTAC AGATTCTTGA CTCTCTGAGT GAAAGTTGTA CCCCTTCAGA CACCAGCACC 
CCAAGAAGAG GCCGTAGACA GCTTCCTTCT GTGCCTCCAA CTCCTAGACC CCTTATCTCT 
TTCTCCTCGT TAATCCGCCG GCAGAAACAC AGCCCCCCTC CTCAAAATAA AAATGATACT 
ATCCTTCCTA GTGAGCCTGG TCTGACTGAG TCTTCCTGCT CCCTTGGGGG TCATCAGAGC 
TCCCAGTCCA CACCACAGCA TTATATCTCA GAGCCCTACC TGGCACTGCA TGAGGAGATG 
CAAAGCTCAG ACTGCCCAGC AGAGGACACA CTCACCTTTG AGGTTGCTGT GGCTACTAGT 
CTGGGCCGCT CGAACACCAT TGGCTCTGCC CCTCTACGTC ATGGCTGGCA AATGCCCAAT 
GGTCACTACC GGCGTCGAAG GAAGGGAGGA ATTCCACCAG TGATACCCTC CAGAGAGTTA 
AGTGACACAG AAGAGGATGA TCGATGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1e ( XM_031900981.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756841.1 Xenopus tropicalis calcium channel, voltage-dependent, R type, alpha 1E subunit (cacna1e), transcript variant X4, mRNA.

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CloneID	OXa57232
Clone ID Related Accession (Same CDS sequence)	XM_031900981.1
Accession Version	XM_031900981.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTATTAA AGATGCTGGG ATTAAGCTCC TGTTCAACCA GTGCTCATAC ACTCAATGAG 
CCTGACCAGT CAAGCACACT AGCCATACCA GCATTGGATG CCGACACTCA GAGATATGTC 
TTGGCCCTTC CAGAAGCAGA GACATGGCGC AAGCATCGAT TGGGCCTGAC ACAGGCTACT 
CACTCTTGCA ATCTTCTAGA ACCTGAGACA CTTTCTGAAA CCCTAGTTTC ACGCACTGCA 
AGTTACGATG CCTTGTATGA CCTGCGAGCA AGAGAAGCGG AGTCTAACTT CCCATTTGAA 
GGCAGCAGCA TGTTTGAGGT GACGTCTGGG ATGGATTCTG AGTTTCCTTT AAGCCCCCAT 
GTTATAAAGC GAAGGAGAGG AGGCCTGATA GAACAAAAGG ATATCATCAA GGCCCACGAA 
GCTCATAAGA TTCAGAGTAC TCCCCAGGCT CGACGGAAGG AATGGGAGAT GGCTCGTTTC 
GGAGAGGCTG TGACTGGCAG GTTGAGCTCC GCAGATGGGG GCTCAGAGAG GAGCAAGACC 
CTACAAGGTT CCCCAGCTGC ACCTGTTGGG ACCCAAGCAG CTTTCAAGCA ATCCAAAGCC 
CAGAGGGCAA GGACCATGGC TTTATACAAC CCCATCCCTG TCCGACAGAA CTGCTTCACC 
GTGAACAGGT CTCTCTTCAT TTTCGGAGAA GATAATATTA TCAGGAAATA CGCCAAGAAG 
CTCATTGATT GGCCTCCTTT TGAATACATG ATCCTTGCCA CAATTATTGC AAACTGCATT 
GTCCTGGCTT TGGAGCAGCA TCTTCCAGGC GATGACAAGA CTCCAATGTC TCGCAGATTG 
GAAAAGACTG AGCCCTATTT CATTGGGATA TTTTGCTTTG AAGCTGGAAT TAAAATTGTC 
GCTCTGGGAC TGGTTTTTCA CAAAGGTTCT TATCTTCGGA ATGGCTGGAA TGTCATGGAC 
TTTATAGTAG TGCTCAGTGG CATCCTGGCC ACAGCTGGAA CTCACTTCAA TACTCACGTG 
GACCTGCGTA CTTTGCGGGC AGTGCGTGTT CTCAGGCCGT TGAAGCTGGT CTCGGGGATT 
CCCAGTTTAC AGATTGTGTT GAAGTCAATC ATGAAGGCGA TGGTCCCTCT GCTACAGATT 
GGATTGTTGC TCTTCTTTGC CATCCTTATG TTTGCTATCA TCGGCCTTGA GTTTTACAGT 
GGAAAGCTAC ACAAGGCCTG TTACAGGAAC AACTCTGGAA TGGAGCTAGA CCCCATGGAT 
CCACCTCACC CTTGTGGAGT GCCCCGCTGC CCAGCTGGGT ATGAGTGCAA GGAATGGATC 
GGACCCAATG ATGGAATAAC ACAGTTTGAC AACATCCTAT TTGCGGTGCT GACAGTCTTC 
CAGTGTATAA CTATGGAGGG CTGGACCACA GTGCTGTACA ATACCAATGA TGCCTTAGGA 
GCCACATGGA ACTGGCTGTA CTTCATTCCC CTCATTATCA TCGGATCCTT CTTTGTCCTT 
AATCTTGTCC TTGGAGTGCT GTCCGGTGAA TTTGCCAAAG AGAGGGAAAG AGTGGAAAAC 
CGCCGGGCTT TCATGAAGTT GCGCAGGCAG CAGCAGATTG AGAGGGAGTT GAATGGCTAT 
CGGGCATGGA TAGATAAAGC TGAGGAAGTC ATGTTGGCAG AAGAAAACAA AAATTCTGGG 
ACCTCTGCTT TAGAAGTTCT TAGGCGAGCA ACCATTAAAC GAAGTAGAAC AGAAGCCATG 
AACCGGGACT CTACTGAGGA CCATTGTGTT GACATATCCT CTGTTGGCAC CCCCCTTGCT 
AGAGCAAGCA TCAAAAGTAC CAAAATGGAT GGGGCATCAT ATTTTCGGCA CAAGGAAAAA 
CTATTGCGTG TCTCCGTTCG TCACATGATC AAATCCCAGG TTTTCTACTG GATGGTCCTG 
GGAGTGGTAG CACTTAACAC TTTCTGTGTG GCTATTGTCC ATCACAACCA GACCCTGTGG 
CTCTCAAATG TTCTTTACTA TGCAGAATTT CTGTTTCTTG GGTTATTTTT GGTGGAGATG 
GCTTTTAAAA TGTATGGAAT GGGTCCACGT CTTTACTTTC ACTCATCCTT CAACTGCTTT 
GATTGCGGGG TCACAATAGG TAGTATATTT GAAGTACTTT GGGCCATATT TAGACCAGGA 
ACTTCCTTTG GAATCAGTGT CTTAAGAGCT CTCCGACTTT TGAGAATCTT CAAAATAACA 
AAATACTGGG CCTCACTACG AAACCTTGTA GTATCATTGA TGAATTCTAT GAAATCCATC 
ATCAGCCTGC TCTTCCTTCT ATTCCTCTTC ATAGTTGTTT TTGCTCTCCT TGGAATGCAG 
TTATATGGTG GCAGATTCAA TTTTAATGAT GGCACTCCTT CTGCAAACTT TGATACATTC 
CCTGCAGCCA TCATGACTGT ATTCCAGATT CTGACAGGAG AGGATTGGAA TGAAGTGATG 
TACAATGGTA TTCGCTCCCA AGGTGGTGTT CGATCCGGAA TGTGGTCTTC CATATACTTT 
ATTGTCCTTA CCCTTTTTGG AAATTACACA CTGCTAAATG TATTTCTTGC CATTGCTGTG 
GACAACCTGG CTAATGCACA GGAGTTAACT AAGGATGAGC AAGAAGAAGA GGAAGCTTTC 
AATCAGAAGC ATGCACTGCA AAAAGCGAAG GAGGTCAGCC CCATGTCTGC ACCTAATGCA 
TCATGTATTG AGAAGGAGCG CAGAAAACGG CACACATTGT CTATATGGGA GCAGCGCACA 
AGCCAGCTAA GGAGGCACAT ACAGATGTCT AGTCAAGAAG CTCTCCAGAG TGAAGAACTG 
GCAGCATTGA ATCCACACTC CAGTATCTAT CGCAAGACAA AGGAAGCTGC TGAACAAAAC 
ACAGAAAAAG AAGAAGTGCA TCAAGACGCA AAACTGGCAA TAGACCCAGT TGAAGGACCA 
CCAGAAGGTG AAGGAAGCCC TTCTAATAGA AATCTCAGGG AAAACTGGCG CCAGAAGTCC 
AGGCATGGAA ATTCTGAAAA CACTGAGCCA AATGGAACAA CTCCAGAAGA AGCAATGCGC 
CGCAGACAAA GTCAGAGACG CAGCCGTCAT AGAAGAGTTA GAACTGAAGG TCGTGAGCAG 
TGTAGCAATC CAAACAGTAC CTCAGCAAGC AGAGAAGGTG GTCTTGACAA AGTCTCTGCA 
GAGGATGGAA GAACAATTCC AGAAGAGAAG AGCAATGGAG AAGCCAAAGA AGTCAAAGGG 
GTTGATGATG AGCCTCTAAA GATTTGTATA TCTTCAAGAA ATTGCGAGGA CAGAAATATC 
AGGACTTTGG GTTGTGAGGG TATTGCCTTG AACGATACAC CCCTGCAGGA TGTGAACAGC 
CAAGAGAAGG ACAGTAGCCC TCCTGATTTG GATTGCAGTG AGCAGGCTCT GTTGGGCAGC 
ATTCAGAGCC AGGAAAGCCC TAATCCAACC AGCTGCAGTT TGCCAGATGT GTCCAGCAGC 
ACCGAGAGAG TCACAGAGAG CACCGTCATT ACCTTACCAG ATACTGGAAT GCCCACCGAC 
TGCACCGTGG TACAGATGAG TGAAGGCAGG GATGGAGAAG TCAACTTTGG GTCTTCGCAG 
CAAGAAAAAG AAGAGGAGGA AGCTGAGGAT GGAGAAGAAA GAACTATAAA AAGAAAAGAG 
AAATTGTTGG AGTCCCCCAA AGCCATTGTA CCGTACAGTT CAATGCTCAT CTTCAGCTCC 
ACCAATCCCA TAAGAAAAGT ATGTCATTAC ATTGTTAACC TACGATACTT TGAGATGAGC 
ATCCTGCTGG TAATAGCAGC AAGTAGTATA GCCTTAGCAG CGGAGGACCC TGTACTGACC 
AACTCGGACC GCAACAAAGT TCTTCGCTAT TTTGACTATG TTTTCACAGG GGTCTTCACT 
TTTGAGATGA TTATAAAGAT GATTGACCAA GGTTTGATTT TACACGAGGG CTCCTACTTT 
CGTGACCTAT GGAACATCTT GGATTTTGTT GTTGTTGTGG GAGCCTTAAT TGCGTTTGCC 
TTAGCGAATG TTTTGGGGAC TAACAAAGGC CGGGACATCA AAACCATTAA GTCTCTACGT 
GTGTTACGGG TGCTGCGTCC ACTAAAAACA ATCAAACGCC TGCCAAAACT GAAGGCAGTC 
TTTGATTGTG TGGTTAACTC TTTAAAGAAT GTCTTTAATA TTCTCATCGT ATACAAACTC 
TTCATGTTCA TATTTGCTGT AATCGCTGTC CAGCTGTTTA AGGGCAAGTT CTTCTATTGT 
ACAGACAGCT CAAAGGAGAC AGAGAAAGAG TGCATAGGGA GTTACATCGA TCACGAAAAG 
AACAAGATGG AGGTGAAGCG GAGAGAGTGG AAAAGACATG ATTTCCATTA TGACAACATA 
ATATGGGCTC TTCTTACCCT CTTTACTGTA TCAACAGGGG AAGGCTGGCC TCAGGTTCTG 
CAGCACTCTG TTGATATAAC TGAAGAAGAT AGGGGTCCAA GCCGAAGCAA CCGTATGGAG 
ATGTCCATTT TCTATGTTAT CTACTTTGTG GTCTTCCCCT TCTTCTTTGT AAATATATTT 
GTGGCTCTGA TTATCATCAC ATTCCAGGAG CAAGGAGACA AGATGATGGA GGAATGTTCC 
CTAGAAAAGA ATGAGAGAGC CTGCATAGAC TTTGCAATAA GTGCTAAGCC ACTTACACGA 
TATATGCCAC AGAACAGGCA CACATTCCAG TACCGGGTCT GGCACTTCGT GGTCTCCCCA 
TCATTTGAAT ACACTATCAT GGCTATGATT GCACTAAACA CAGTGGTACT CATGATGAAG 
TATTATTCTG CGCCATACAC ATATGAGCTT GCATTAAAGT ATCTCAATAT TGCATTCACA 
ATGCTATTTT CTCTGGAGTG CATCCTGAAA ATAATTGCAT TTGGGTTTTT GAATTACTTT 
CGGGATACTT GGAACATTTT TGACTTCATT ACTGTAATTG GTAGCATCAC GGAAATTATT 
CTAACAGATA GTAAGTTGGT GAACACCAGC AGCTTTAATA TGAGCTTTCT GAAGCTGTTC 
CGAGCGGCGC GACTTATTAA ACTTCTCCGG CAAGGGTACA CCATCCGGAT CCTGCTGTGG 
ACTTTTGTTC AGTCCTTTAA GGCACTTCCT TATGTGTGTC TTCTCATTGC AATGTTGTTC 
TTCATCTATG CCATTATTGG GATGCAGGTG TTTGGCAATA TCCGCCTGGA AGAGGACAGT 
CATATTAATA GGCACAACAA TTTTAGAAGC TTCTTTAGTT CTCTTATGTT ACTCTTCAGG 
AGTGCCACAG GAGAAGCGTG GCAGGAGATC ATGCTTTCAT GCCTTAGTGA TAAGGGTTGT 
GAGCCAGACA CCAGTGCAGC CTCTGGACAC AACGAGAATG AGCGCTGTGG AACTGAACTT 
GCATATGTTT ATTTTGTTTC ATTCATTTTC TTCTGCTCCT TCCTCATGCT AAATCTGTTT 
GTGGCTGTCA TTATGGACAA CTTTGAGTAC TTGACACGGG ACTCTTCCAT TTTGGGACCT 
CATCACTTGG ATGAGTTTGT TCGGATATGG GCTGAATATG ATCGAGCAGC ATGTGGCCGT 
ATCCATTACA CAGAGATGTA TGACATGCTG ACTCTCATGT CACCACCTCT AGGCCTTGGA 
AAGAGATGCC CTAACAAAGT GGCTTATAAG AGATTGGTCC TGATGAATAT GCCAGTGTCG 
GAGGACATGA CGGTTCATTT CACATCAACT CTTATGGCCC TAATACGCAC GGCACTGGAT 
ATCAAAATAG CTAAAGGAGG TGCTGACAGG CAACAGCTGG ACTCAGAGCT GCAGAAAGAT 
ATACTTGCCA TTTGGCCAAA TCTCTCCCAG AAAATGATCG ACTTACTTGT GCCAATGCCA 
AGAGCCTCTG ATCTGACGGT GGGGAAGATT TACGCTGCAA TGATGATCAT GGATTACTAT 
AAACAGAGCA AAGCGAAAAA ACAAAGGCAG CAGCTGGAGG AGCAGAAAAA TGCACCTATG 
TTTCAGAGAA TGGAGGCTTC TGCCCTCCCT GAGGAGATCT TATCCAATGC CAAAGCTTTA 
CCATACCTTC AGCAAGACAC TCTATCAGGC CTGGGTGGAT TTCCTTCACT GAGTCCCTTG 
TCCCCAGAAG AAGTATTCCA GCCGCCATGC CTAGACGGGG AAGAGGAGGA AGACTTTGAA 
GAGCAGCTGT CTCTGGTCCT GTCAGACGTA AGCTCCATGC GTCGCTCCTT CTCCACAACT 
GCAGAGAAGC GAGTGCGCAG CTCGTGGCTA GATGAGTTCA CTATGGACAG GAGTAGCACT 
GACTGTGCAT ACAGATACCA GAGGCGCAGT TATCATGCAT CCTTAAGACT GTCTGCACAT 
CGCCTGAATA CAGATACAGG TCACTGGTCA GATAATCACC GTTCTGGCGG AAGGGAGCGA 
GGAAGGTCCA AAGAGAGAAA ACACTTGCTG TCACCCGATC CTTCCCGCTG TAATTCTGAG 
GAAAGGAGCC CACAAGCTGC CTGTGAACAC CGAGATCACC AACTGTCCCC TGGGCATACT 
CCCAGTAGTG TACAGATTCT TGACTCTCTG AGTGAAAGTT GTACCCCTTC AGACACCAGC 
ACCCCAAGAA GAGGCCGTAG ACAGCTTCCT TCTGTGCCTC CAACTCCTAG ACCCCTTATC 
TCTTTCTCCT CGTTAATCCG CCGGCAGAAA CACAGCCCCC CTCCTCAAAA TAAAAATGAT 
ACTATCCTTC CTAGTGAGCC TGGTCTGACT GAGTCTTCCT GCTCCCTTGG GGGTCATCAG 
AGCTCCCAGT CCACACCACA GCATTATATC TCAGAGCCCT ACCTGGCACT GCATGAGGAG 
ATGCAAAGCT CAGACTGCCC AGCAGAGGAC ACACTCACCT TTGAGGTTGC TGTGGCTACT 
AGTCTGGGCC GCTCGAACAC CATTGGCTCT GCCCCTCTAC GTCATGGCTG GCAAATGCCC 
AATGGTCACT ACCGGCGTCG AAGGAAGGGA GGAATTCCAC CAGTGATACC CTCCAGAGAG 
TTAAGTGACA CAGAAGAGGA TGATCGATGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756841.1
CDS	284..7336
Translation	MVLKMLGLSSCSTSAHTLNEPDQSSTLAIPALDADTQRYVLALPEAETWRKHRLGLTQATHSCNLLEPETLSETLVSRTASYDALYDLRAREAESNFPFEGSSMFEVTSGMDSEFPLSPHVIKRRRGGLIEQKDIIKAHEAHKIQSTPQARRKEWEMARFGEAVTGRLSSADGGSERSKTLQGSPAAPVGTQAAFKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIIRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPGDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGLVFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHKACYRNNSGMELDPMDPPHPCGVPRCPAGYECKEWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNSGTSALEVLRRATIKRSRTEAMNRDSTEDHCVDISSVGTPLARASIKSTKMDGASYFRHKEKLLRVSVRHMIKSQVFYWMVLGVVALNTFCVAIVHHNQTLWLSNVLYYAEFLFLGLFLVEMAFKMYGMGPRLYFHSSFNCFDCGVTIGSIFEVLWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMNSMKSIISLLFLLFLFIVVFALLGMQLYGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVRSGMWSSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNASCIEKERRKRHTLSIWEQRTSQLRRHIQMSSQEALQSEELAALNPHSSIYRKTKEAAEQNTEKEEVHQDAKLAIDPVEGPPEGEGSPSNRNLRENWRQKSRHGNSENTEPNGTTPEEAMRRRQSQRRSRHRRVRTEGREQCSNPNSTSASREGGLDKVSAEDGRTIPEEKSNGEAKEVKGVDDEPLKICISSRNCEDRNIRTLGCEGIALNDTPLQDVNSQEKDSSPPDLDCSEQALLGSIQSQESPNPTSCSLPDVSSSTERVTESTVITLPDTGMPTDCTVVQMSEGRDGEVNFGSSQQEKEEEEAEDGEERTIKRKEKLLESPKAIVPYSSMLIFSSTNPIRKVCHYIVNLRYFEMSILLVIAASSIALAAEDPVLTNSDRNKVLRYFDYVFTGVFTFEMIIKMIDQGLILHEGSYFRDLWNILDFVVVVGALIAFALANVLGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKETEKECIGSYIDHEKNKMEVKRREWKRHDFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDITEEDRGPSRSNRMEMSIFYVIYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPYTYELALKYLNIAFTMLFSLECILKIIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSSFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIRLEEDSHINRHNNFRSFFSSLMLLFRSATGEAWQEIMLSCLSDKGCEPDTSAASGHNENERCGTELAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRIWAEYDRAACGRIHYTEMYDMLTLMSPPLGLGKRCPNKVAYKRLVLMNMPVSEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKDILAIWPNLSQKMIDLLVPMPRASDLTVGKIYAAMMIMDYYKQSKAKKQRQQLEEQKNAPMFQRMEASALPEEILSNAKALPYLQQDTLSGLGGFPSLSPLSPEEVFQPPCLDGEEEEDFEEQLSLVLSDVSSMRRSFSTTAEKRVRSSWLDEFTMDRSSTDCAYRYQRRSYHASLRLSAHRLNTDTGHWSDNHRSGGRERGRSKERKHLLSPDPSRCNSEERSPQAACEHRDHQLSPGHTPSSVQILDSLSESCTPSDTSTPRRGRRQLPSVPPTPRPLISFSSLIRRQKHSPPPQNKNDTILPSEPGLTESSCSLGGHQSSQSTPQHYISEPYLALHEEMQSSDCPAEDTLTFEVAVATSLGRSNTIGSAPLRHGWQMPNGHYRRRRKGGIPPVIPSRELSDTEEDDRC

Target ORF information:

RefSeq Version	XM_031900981.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calcium channel, voltage-dependent, R type, alpha 1E subunit (cacna1e), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031900981.1

ORF Insert Sequence:

ATGGTATTAA AGATGCTGGG ATTAAGCTCC TGTTCAACCA GTGCTCATAC ACTCAATGAG 
CCTGACCAGT CAAGCACACT AGCCATACCA GCATTGGATG CCGACACTCA GAGATATGTC 
TTGGCCCTTC CAGAAGCAGA GACATGGCGC AAGCATCGAT TGGGCCTGAC ACAGGCTACT 
CACTCTTGCA ATCTTCTAGA ACCTGAGACA CTTTCTGAAA CCCTAGTTTC ACGCACTGCA 
AGTTACGATG CCTTGTATGA CCTGCGAGCA AGAGAAGCGG AGTCTAACTT CCCATTTGAA 
GGCAGCAGCA TGTTTGAGGT GACGTCTGGG ATGGATTCTG AGTTTCCTTT AAGCCCCCAT 
GTTATAAAGC GAAGGAGAGG AGGCCTGATA GAACAAAAGG ATATCATCAA GGCCCACGAA 
GCTCATAAGA TTCAGAGTAC TCCCCAGGCT CGACGGAAGG AATGGGAGAT GGCTCGTTTC 
GGAGAGGCTG TGACTGGCAG GTTGAGCTCC GCAGATGGGG GCTCAGAGAG GAGCAAGACC 
CTACAAGGTT CCCCAGCTGC ACCTGTTGGG ACCCAAGCAG CTTTCAAGCA ATCCAAAGCC 
CAGAGGGCAA GGACCATGGC TTTATACAAC CCCATCCCTG TCCGACAGAA CTGCTTCACC 
GTGAACAGGT CTCTCTTCAT TTTCGGAGAA GATAATATTA TCAGGAAATA CGCCAAGAAG 
CTCATTGATT GGCCTCCTTT TGAATACATG ATCCTTGCCA CAATTATTGC AAACTGCATT 
GTCCTGGCTT TGGAGCAGCA TCTTCCAGGC GATGACAAGA CTCCAATGTC TCGCAGATTG 
GAAAAGACTG AGCCCTATTT CATTGGGATA TTTTGCTTTG AAGCTGGAAT TAAAATTGTC 
GCTCTGGGAC TGGTTTTTCA CAAAGGTTCT TATCTTCGGA ATGGCTGGAA TGTCATGGAC 
TTTATAGTAG TGCTCAGTGG CATCCTGGCC ACAGCTGGAA CTCACTTCAA TACTCACGTG 
GACCTGCGTA CTTTGCGGGC AGTGCGTGTT CTCAGGCCGT TGAAGCTGGT CTCGGGGATT 
CCCAGTTTAC AGATTGTGTT GAAGTCAATC ATGAAGGCGA TGGTCCCTCT GCTACAGATT 
GGATTGTTGC TCTTCTTTGC CATCCTTATG TTTGCTATCA TCGGCCTTGA GTTTTACAGT 
GGAAAGCTAC ACAAGGCCTG TTACAGGAAC AACTCTGGAA TGGAGCTAGA CCCCATGGAT 
CCACCTCACC CTTGTGGAGT GCCCCGCTGC CCAGCTGGGT ATGAGTGCAA GGAATGGATC 
GGACCCAATG ATGGAATAAC ACAGTTTGAC AACATCCTAT TTGCGGTGCT GACAGTCTTC 
CAGTGTATAA CTATGGAGGG CTGGACCACA GTGCTGTACA ATACCAATGA TGCCTTAGGA 
GCCACATGGA ACTGGCTGTA CTTCATTCCC CTCATTATCA TCGGATCCTT CTTTGTCCTT 
AATCTTGTCC TTGGAGTGCT GTCCGGTGAA TTTGCCAAAG AGAGGGAAAG AGTGGAAAAC 
CGCCGGGCTT TCATGAAGTT GCGCAGGCAG CAGCAGATTG AGAGGGAGTT GAATGGCTAT 
CGGGCATGGA TAGATAAAGC TGAGGAAGTC ATGTTGGCAG AAGAAAACAA AAATTCTGGG 
ACCTCTGCTT TAGAAGTTCT TAGGCGAGCA ACCATTAAAC GAAGTAGAAC AGAAGCCATG 
AACCGGGACT CTACTGAGGA CCATTGTGTT GACATATCCT CTGTTGGCAC CCCCCTTGCT 
AGAGCAAGCA TCAAAAGTAC CAAAATGGAT GGGGCATCAT ATTTTCGGCA CAAGGAAAAA 
CTATTGCGTG TCTCCGTTCG TCACATGATC AAATCCCAGG TTTTCTACTG GATGGTCCTG 
GGAGTGGTAG CACTTAACAC TTTCTGTGTG GCTATTGTCC ATCACAACCA GACCCTGTGG 
CTCTCAAATG TTCTTTACTA TGCAGAATTT CTGTTTCTTG GGTTATTTTT GGTGGAGATG 
GCTTTTAAAA TGTATGGAAT GGGTCCACGT CTTTACTTTC ACTCATCCTT CAACTGCTTT 
GATTGCGGGG TCACAATAGG TAGTATATTT GAAGTACTTT GGGCCATATT TAGACCAGGA 
ACTTCCTTTG GAATCAGTGT CTTAAGAGCT CTCCGACTTT TGAGAATCTT CAAAATAACA 
AAATACTGGG CCTCACTACG AAACCTTGTA GTATCATTGA TGAATTCTAT GAAATCCATC 
ATCAGCCTGC TCTTCCTTCT ATTCCTCTTC ATAGTTGTTT TTGCTCTCCT TGGAATGCAG 
TTATATGGTG GCAGATTCAA TTTTAATGAT GGCACTCCTT CTGCAAACTT TGATACATTC 
CCTGCAGCCA TCATGACTGT ATTCCAGATT CTGACAGGAG AGGATTGGAA TGAAGTGATG 
TACAATGGTA TTCGCTCCCA AGGTGGTGTT CGATCCGGAA TGTGGTCTTC CATATACTTT 
ATTGTCCTTA CCCTTTTTGG AAATTACACA CTGCTAAATG TATTTCTTGC CATTGCTGTG 
GACAACCTGG CTAATGCACA GGAGTTAACT AAGGATGAGC AAGAAGAAGA GGAAGCTTTC 
AATCAGAAGC ATGCACTGCA AAAAGCGAAG GAGGTCAGCC CCATGTCTGC ACCTAATGCA 
TCATGTATTG AGAAGGAGCG CAGAAAACGG CACACATTGT CTATATGGGA GCAGCGCACA 
AGCCAGCTAA GGAGGCACAT ACAGATGTCT AGTCAAGAAG CTCTCCAGAG TGAAGAACTG 
GCAGCATTGA ATCCACACTC CAGTATCTAT CGCAAGACAA AGGAAGCTGC TGAACAAAAC 
ACAGAAAAAG AAGAAGTGCA TCAAGACGCA AAACTGGCAA TAGACCCAGT TGAAGGACCA 
CCAGAAGGTG AAGGAAGCCC TTCTAATAGA AATCTCAGGG AAAACTGGCG CCAGAAGTCC 
AGGCATGGAA ATTCTGAAAA CACTGAGCCA AATGGAACAA CTCCAGAAGA AGCAATGCGC 
CGCAGACAAA GTCAGAGACG CAGCCGTCAT AGAAGAGTTA GAACTGAAGG TCGTGAGCAG 
TGTAGCAATC CAAACAGTAC CTCAGCAAGC AGAGAAGGTG GTCTTGACAA AGTCTCTGCA 
GAGGATGGAA GAACAATTCC AGAAGAGAAG AGCAATGGAG AAGCCAAAGA AGTCAAAGGG 
GTTGATGATG AGCCTCTAAA GATTTGTATA TCTTCAAGAA ATTGCGAGGA CAGAAATATC 
AGGACTTTGG GTTGTGAGGG TATTGCCTTG AACGATACAC CCCTGCAGGA TGTGAACAGC 
CAAGAGAAGG ACAGTAGCCC TCCTGATTTG GATTGCAGTG AGCAGGCTCT GTTGGGCAGC 
ATTCAGAGCC AGGAAAGCCC TAATCCAACC AGCTGCAGTT TGCCAGATGT GTCCAGCAGC 
ACCGAGAGAG TCACAGAGAG CACCGTCATT ACCTTACCAG ATACTGGAAT GCCCACCGAC 
TGCACCGTGG TACAGATGAG TGAAGGCAGG GATGGAGAAG TCAACTTTGG GTCTTCGCAG 
CAAGAAAAAG AAGAGGAGGA AGCTGAGGAT GGAGAAGAAA GAACTATAAA AAGAAAAGAG 
AAATTGTTGG AGTCCCCCAA AGCCATTGTA CCGTACAGTT CAATGCTCAT CTTCAGCTCC 
ACCAATCCCA TAAGAAAAGT ATGTCATTAC ATTGTTAACC TACGATACTT TGAGATGAGC 
ATCCTGCTGG TAATAGCAGC AAGTAGTATA GCCTTAGCAG CGGAGGACCC TGTACTGACC 
AACTCGGACC GCAACAAAGT TCTTCGCTAT TTTGACTATG TTTTCACAGG GGTCTTCACT 
TTTGAGATGA TTATAAAGAT GATTGACCAA GGTTTGATTT TACACGAGGG CTCCTACTTT 
CGTGACCTAT GGAACATCTT GGATTTTGTT GTTGTTGTGG GAGCCTTAAT TGCGTTTGCC 
TTAGCGAATG TTTTGGGGAC TAACAAAGGC CGGGACATCA AAACCATTAA GTCTCTACGT 
GTGTTACGGG TGCTGCGTCC ACTAAAAACA ATCAAACGCC TGCCAAAACT GAAGGCAGTC 
TTTGATTGTG TGGTTAACTC TTTAAAGAAT GTCTTTAATA TTCTCATCGT ATACAAACTC 
TTCATGTTCA TATTTGCTGT AATCGCTGTC CAGCTGTTTA AGGGCAAGTT CTTCTATTGT 
ACAGACAGCT CAAAGGAGAC AGAGAAAGAG TGCATAGGGA GTTACATCGA TCACGAAAAG 
AACAAGATGG AGGTGAAGCG GAGAGAGTGG AAAAGACATG ATTTCCATTA TGACAACATA 
ATATGGGCTC TTCTTACCCT CTTTACTGTA TCAACAGGGG AAGGCTGGCC TCAGGTTCTG 
CAGCACTCTG TTGATATAAC TGAAGAAGAT AGGGGTCCAA GCCGAAGCAA CCGTATGGAG 
ATGTCCATTT TCTATGTTAT CTACTTTGTG GTCTTCCCCT TCTTCTTTGT AAATATATTT 
GTGGCTCTGA TTATCATCAC ATTCCAGGAG CAAGGAGACA AGATGATGGA GGAATGTTCC 
CTAGAAAAGA ATGAGAGAGC CTGCATAGAC TTTGCAATAA GTGCTAAGCC ACTTACACGA 
TATATGCCAC AGAACAGGCA CACATTCCAG TACCGGGTCT GGCACTTCGT GGTCTCCCCA 
TCATTTGAAT ACACTATCAT GGCTATGATT GCACTAAACA CAGTGGTACT CATGATGAAG 
TATTATTCTG CGCCATACAC ATATGAGCTT GCATTAAAGT ATCTCAATAT TGCATTCACA 
ATGCTATTTT CTCTGGAGTG CATCCTGAAA ATAATTGCAT TTGGGTTTTT GAATTACTTT 
CGGGATACTT GGAACATTTT TGACTTCATT ACTGTAATTG GTAGCATCAC GGAAATTATT 
CTAACAGATA GTAAGTTGGT GAACACCAGC AGCTTTAATA TGAGCTTTCT GAAGCTGTTC 
CGAGCGGCGC GACTTATTAA ACTTCTCCGG CAAGGGTACA CCATCCGGAT CCTGCTGTGG 
ACTTTTGTTC AGTCCTTTAA GGCACTTCCT TATGTGTGTC TTCTCATTGC AATGTTGTTC 
TTCATCTATG CCATTATTGG GATGCAGGTG TTTGGCAATA TCCGCCTGGA AGAGGACAGT 
CATATTAATA GGCACAACAA TTTTAGAAGC TTCTTTAGTT CTCTTATGTT ACTCTTCAGG 
AGTGCCACAG GAGAAGCGTG GCAGGAGATC ATGCTTTCAT GCCTTAGTGA TAAGGGTTGT 
GAGCCAGACA CCAGTGCAGC CTCTGGACAC AACGAGAATG AGCGCTGTGG AACTGAACTT 
GCATATGTTT ATTTTGTTTC ATTCATTTTC TTCTGCTCCT TCCTCATGCT AAATCTGTTT 
GTGGCTGTCA TTATGGACAA CTTTGAGTAC TTGACACGGG ACTCTTCCAT TTTGGGACCT 
CATCACTTGG ATGAGTTTGT TCGGATATGG GCTGAATATG ATCGAGCAGC ATGTGGCCGT 
ATCCATTACA CAGAGATGTA TGACATGCTG ACTCTCATGT CACCACCTCT AGGCCTTGGA 
AAGAGATGCC CTAACAAAGT GGCTTATAAG AGATTGGTCC TGATGAATAT GCCAGTGTCG 
GAGGACATGA CGGTTCATTT CACATCAACT CTTATGGCCC TAATACGCAC GGCACTGGAT 
ATCAAAATAG CTAAAGGAGG TGCTGACAGG CAACAGCTGG ACTCAGAGCT GCAGAAAGAT 
ATACTTGCCA TTTGGCCAAA TCTCTCCCAG AAAATGATCG ACTTACTTGT GCCAATGCCA 
AGAGCCTCTG ATCTGACGGT GGGGAAGATT TACGCTGCAA TGATGATCAT GGATTACTAT 
AAACAGAGCA AAGCGAAAAA ACAAAGGCAG CAGCTGGAGG AGCAGAAAAA TGCACCTATG 
TTTCAGAGAA TGGAGGCTTC TGCCCTCCCT GAGGAGATCT TATCCAATGC CAAAGCTTTA 
CCATACCTTC AGCAAGACAC TCTATCAGGC CTGGGTGGAT TTCCTTCACT GAGTCCCTTG 
TCCCCAGAAG AAGTATTCCA GCCGCCATGC CTAGACGGGG AAGAGGAGGA AGACTTTGAA 
GAGCAGCTGT CTCTGGTCCT GTCAGACGTA AGCTCCATGC GTCGCTCCTT CTCCACAACT 
GCAGAGAAGC GAGTGCGCAG CTCGTGGCTA GATGAGTTCA CTATGGACAG GAGTAGCACT 
GACTGTGCAT ACAGATACCA GAGGCGCAGT TATCATGCAT CCTTAAGACT GTCTGCACAT 
CGCCTGAATA CAGATACAGG TCACTGGTCA GATAATCACC GTTCTGGCGG AAGGGAGCGA 
GGAAGGTCCA AAGAGAGAAA ACACTTGCTG TCACCCGATC CTTCCCGCTG TAATTCTGAG 
GAAAGGAGCC CACAAGCTGC CTGTGAACAC CGAGATCACC AACTGTCCCC TGGGCATACT 
CCCAGTAGTG TACAGATTCT TGACTCTCTG AGTGAAAGTT GTACCCCTTC AGACACCAGC 
ACCCCAAGAA GAGGCCGTAG ACAGCTTCCT TCTGTGCCTC CAACTCCTAG ACCCCTTATC 
TCTTTCTCCT CGTTAATCCG CCGGCAGAAA CACAGCCCCC CTCCTCAAAA TAAAAATGAT 
ACTATCCTTC CTAGTGAGCC TGGTCTGACT GAGTCTTCCT GCTCCCTTGG GGGTCATCAG 
AGCTCCCAGT CCACACCACA GCATTATATC TCAGAGCCCT ACCTGGCACT GCATGAGGAG 
ATGCAAAGCT CAGACTGCCC AGCAGAGGAC ACACTCACCT TTGAGGTTGC TGTGGCTACT 
AGTCTGGGCC GCTCGAACAC CATTGGCTCT GCCCCTCTAC GTCATGGCTG GCAAATGCCC 
AATGGTCACT ACCGGCGTCG AAGGAAGGGA GGAATTCCAC CAGTGATACC CTCCAGAGAG 
TTAAGTGACA CAGAAGAGGA TGATCGATGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1e ( XM_031900982.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756842.1 Xenopus tropicalis calcium channel, voltage-dependent, R type, alpha 1E subunit (cacna1e), transcript variant X5, mRNA.

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CloneID	OXa57233
Clone ID Related Accession (Same CDS sequence)	XM_031900982.1
Accession Version	XM_031900982.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6783bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTAGCGC CGGCAGGAGC CGGGGAGCAG ATCGGCACAG GAGTGCAGCT TCAACCCGGC 
TATAGGATGA TGGCTCGTTT CGGAGAGGCT GTGACTGGCA GGTTGAGCTC CGCAGATGGG 
GGCTCAGAGA GGAGCAAGAC CCTACAAGGT TCCCCAGCTG CACCTGTTGG GACCCAAGCA 
GCTTTCAAGC AATCCAAAGC CCAGAGGGCA AGGACCATGG CTTTATACAA CCCCATCCCT 
GTCCGACAGA ACTGCTTCAC CGTGAACAGG TCTCTCTTCA TTTTCGGAGA AGATAATATT 
ATCAGGAAAT ACGCCAAGAA GCTCATTGAT TGGCCTCCTT TTGAATACAT GATCCTTGCC 
ACAATTATTG CAAACTGCAT TGTCCTGGCT TTGGAGCAGC ATCTTCCAGG CGATGACAAG 
ACTCCAATGT CTCGCAGATT GGAAAAGACT GAGCCCTATT TCATTGGGAT ATTTTGCTTT 
GAAGCTGGAA TTAAAATTGT CGCTCTGGGA CTGGTTTTTC ACAAAGGTTC TTATCTTCGG 
AATGGCTGGA ATGTCATGGA CTTTATAGTA GTGCTCAGTG GCATCCTGGC CACAGCTGGA 
ACTCACTTCA ATACTCACGT GGACCTGCGT ACTTTGCGGG CAGTGCGTGT TCTCAGGCCG 
TTGAAGCTGG TCTCGGGGAT TCCCAGTTTA CAGATTGTGT TGAAGTCAAT CATGAAGGCG 
ATGGTCCCTC TGCTACAGAT TGGATTGTTG CTCTTCTTTG CCATCCTTAT GTTTGCTATC 
ATCGGCCTTG AGTTTTACAG TGGAAAGCTA CACAAGGCCT GTTACAGGAA CAACTCTGGA 
ATGGAGCTAG ACCCCATGGA TCCACCTCAC CCTTGTGGAG TGCCCCGCTG CCCAGCTGGG 
TATGAGTGCA AGGAATGGAT CGGACCCAAT GATGGAATAA CACAGTTTGA CAACATCCTA 
TTTGCGGTGC TGACAGTCTT CCAGTGTATA ACTATGGAGG GCTGGACCAC AGTGCTGTAC 
AATACCAATG ATGCCTTAGG AGCCACATGG AACTGGCTGT ACTTCATTCC CCTCATTATC 
ATCGGATCCT TCTTTGTCCT TAATCTTGTC CTTGGAGTGC TGTCCGGTGA ATTTGCCAAA 
GAGAGGGAAA GAGTGGAAAA CCGCCGGGCT TTCATGAAGT TGCGCAGGCA GCAGCAGATT 
GAGAGGGAGT TGAATGGCTA TCGGGCATGG ATAGATAAAG CTGAGGAAGT CATGTTGGCA 
GAAGAAAACA AAAATTCTGG GACCTCTGCT TTAGAAGTTC TTAGGCGAGC AACCATTAAA 
CGAAGTAGAA CAGAAGCCAT GAACCGGGAC TCTACTGAGG ACCATTGTGT TGACATATCC 
TCTGTTGGCA CCCCCCTTGC TAGAGCAAGC ATCAAAAGTA CCAAAATGGA TGGGGCATCA 
TATTTTCGGC ACAAGGAAAA ACTATTGCGT GTCTCCGTTC GTCACATGAT CAAATCCCAG 
GTTTTCTACT GGATGGTCCT GGGAGTGGTA GCACTTAACA CTTTCTGTGT GGCTATTGTC 
CATCACAACC AGACCCTGTG GCTCTCAAAT GTTCTTTACT ATGCAGAATT TCTGTTTCTT 
GGGTTATTTT TGGTGGAGAT GGCTTTTAAA ATGTATGGAA TGGGTCCACG TCTTTACTTT 
CACTCATCCT TCAACTGCTT TGATTGCGGG GTCACAATAG GTAGTATATT TGAAGTACTT 
TGGGCCATAT TTAGACCAGG AACTTCCTTT GGAATCAGTG TCTTAAGAGC TCTCCGACTT 
TTGAGAATCT TCAAAATAAC AAAATACTGG GCCTCACTAC GAAACCTTGT AGTATCATTG 
ATGAATTCTA TGAAATCCAT CATCAGCCTG CTCTTCCTTC TATTCCTCTT CATAGTTGTT 
TTTGCTCTCC TTGGAATGCA GTTATATGGT GGCAGATTCA ATTTTAATGA TGGCACTCCT 
TCTGCAAACT TTGATACATT CCCTGCAGCC ATCATGACTG TATTCCAGAT TCTGACAGGA 
GAGGATTGGA ATGAAGTGAT GTACAATGGT ATTCGCTCCC AAGGTGGTGT TCGATCCGGA 
ATGTGGTCTT CCATATACTT TATTGTCCTT ACCCTTTTTG GAAATTACAC ACTGCTAAAT 
GTATTTCTTG CCATTGCTGT GGACAACCTG GCTAATGCAC AGGAGTTAAC TAAGGATGAG 
CAAGAAGAAG AGGAAGCTTT CAATCAGAAG CATGCACTGC AAAAAGCGAA GGAGGTCAGC 
CCCATGTCTG CACCTAATGC ATCATGTATT GAGAAGGAGC GCAGAAAACG GCACACATTG 
TCTATATGGG AGCAGCGCAC AAGCCAGCTA AGGAGGCACA TACAGATGTC TAGTCAAGAA 
GCTCTCCAGA GTGAAGAACT GGCAGCATTG AATCCACACT CCAGTATCTA TCGCAAGACA 
AAGGAAGCTG CTGAACAAAA CACAGAAAAA GAAGAAGTGC ATCAAGACGC AAAACTGGCA 
ATAGACCCAG TTGAAGGACC ACCAGAAGGT GAAGGAAGCC CTTCTAATAG AAATCTCAGG 
GAAAACTGGC GCCAGAAGTC CAGGCATGGA AATTCTGAAA ACACTGAGCC AAATGGAACA 
ACTCCAGAAG AAGCAATGCG CCGCAGACAA AGTCAGAGAC GCAGCCGTCA TAGAAGAGTT 
AGAACTGAAG GTCGTGAGCA GTGTAGCAAT CCAAACAGTA CCTCAGCAAG CAGAGAAGGT 
GGTCTTGACA AAGTCTCTGC AGAGGATGGA AGAACAATTC CAGAAGAGAA GAGCAATGGA 
GAAGCCAAAG AAGTCAAAGG GGTTGATGAT GAGCCTCTAA AGATTTGTAT ATCTTCAAGA 
AATTGCGAGG ACAGAAATAT CAGGACTTTG GGTTGTGAGG GTATTGCCTT GAACGATACA 
CCCCTGCAGG ATGTGAACAG CCAAGAGAAG GACAGTAGCC CTCCTGATTT GGATTGCAGT 
GAGCAGGCTC TGTTGGGCAG CATTCAGAGC CAGGAAAGCC CTAATCCAAC CAGCTGCAGT 
TTGCCAGATG TGTCCAGCAG CACCGAGAGA GTCACAGAGA GCACCGTCAT TACCTTACCA 
GATACTGGAA TGCCCACCGA CTGCACCGTG GTACAGATGA GTGAAGGCAG GGATGGAGAA 
GTCAACTTTG GGTCTTCGCA GCAAGAAAAA GAAGAGGAGG AAGCTGAGGA TGGAGAAGAA 
AGAACTATAA AAAGAAAAGA GAAATTGTTG GAGTCCCCCA AAGCCATTGT ACCGTACAGT 
TCAATGCTCA TCTTCAGCTC CACCAATCCC ATAAGAAAAG TATGTCATTA CATTGTTAAC 
CTACGATACT TTGAGATGAG CATCCTGCTG GTAATAGCAG CAAGTAGTAT AGCCTTAGCA 
GCGGAGGACC CTGTACTGAC CAACTCGGAC CGCAACAAAG TTCTTCGCTA TTTTGACTAT 
GTTTTCACAG GGGTCTTCAC TTTTGAGATG ATTATAAAGA TGATTGACCA AGGTTTGATT 
TTACACGAGG GCTCCTACTT TCGTGACCTA TGGAACATCT TGGATTTTGT TGTTGTTGTG 
GGAGCCTTAA TTGCGTTTGC CTTAGCGAAT GTTTTGGGGA CTAACAAAGG CCGGGACATC 
AAAACCATTA AGTCTCTACG TGTGTTACGG GTGCTGCGTC CACTAAAAAC AATCAAACGC 
CTGCCAAAAC TGAAGGCAGT CTTTGATTGT GTGGTTAACT CTTTAAAGAA TGTCTTTAAT 
ATTCTCATCG TATACAAACT CTTCATGTTC ATATTTGCTG TAATCGCTGT CCAGCTGTTT 
AAGGGCAAGT TCTTCTATTG TACAGACAGC TCAAAGGAGA CAGAGAAAGA GTGCATAGGG 
AGTTACATCG ATCACGAAAA GAACAAGATG GAGGTGAAGC GGAGAGAGTG GAAAAGACAT 
GATTTCCATT ATGACAACAT AATATGGGCT CTTCTTACCC TCTTTACTGT ATCAACAGGG 
GAAGGCTGGC CTCAGGTTCT GCAGCACTCT GTTGATATAA CTGAAGAAGA TAGGGGTCCA 
AGCCGAAGCA ACCGTATGGA GATGTCCATT TTCTATGTTA TCTACTTTGT GGTCTTCCCC 
TTCTTCTTTG TAAATATATT TGTGGCTCTG ATTATCATCA CATTCCAGGA GCAAGGAGAC 
AAGATGATGG AGGAATGTTC CCTAGAAAAG AATGAGAGAG CCTGCATAGA CTTTGCAATA 
AGTGCTAAGC CACTTACACG ATATATGCCA CAGAACAGGC ACACATTCCA GTACCGGGTC 
TGGCACTTCG TGGTCTCCCC ATCATTTGAA TACACTATCA TGGCTATGAT TGCACTAAAC 
ACAGTGGTAC TCATGATGAA GTATTATTCT GCGCCATACA CATATGAGCT TGCATTAAAG 
TATCTCAATA TTGCATTCAC AATGCTATTT TCTCTGGAGT GCATCCTGAA AATAATTGCA 
TTTGGGTTTT TGAATTACTT TCGGGATACT TGGAACATTT TTGACTTCAT TACTGTAATT 
GGTAGCATCA CGGAAATTAT TCTAACAGAT AGTAAGTTGG TGAACACCAG CAGCTTTAAT 
ATGAGCTTTC TGAAGCTGTT CCGAGCGGCG CGACTTATTA AACTTCTCCG GCAAGGGTAC 
ACCATCCGGA TCCTGCTGTG GACTTTTGTT CAGTCCTTTA AGGCACTTCC TTATGTGTGT 
CTTCTCATTG CAATGTTGTT CTTCATCTAT GCCATTATTG GGATGCAGGT GTTTGGCAAT 
ATCCGCCTGG AAGAGGACAG TCATATTAAT AGGCACAACA ATTTTAGAAG CTTCTTTAGT 
TCTCTTATGT TACTCTTCAG GAGTGCCACA GGAGAAGCGT GGCAGGAGAT CATGCTTTCA 
TGCCTTAGTG ATAAGGGTTG TGAGCCAGAC ACCAGTGCAG CCTCTGGACA CAACGAGAAT 
GAGCGCTGTG GAACTGAACT TGCATATGTT TATTTTGTTT CATTCATTTT CTTCTGCTCC 
TTCCTCATGC TAAATCTGTT TGTGGCTGTC ATTATGGACA ACTTTGAGTA CTTGACACGG 
GACTCTTCCA TTTTGGGACC TCATCACTTG GATGAGTTTG TTCGGATATG GGCTGAATAT 
GATCGAGCAG CATGTGGCCG TATCCATTAC ACAGAGATGT ATGACATGCT GACTCTCATG 
TCACCACCTC TAGGCCTTGG AAAGAGATGC CCTAACAAAG TGGCTTATAA GAGATTGGTC 
CTGATGAATA TGCCAGTGTC GGAGGACATG ACGGTTCATT TCACATCAAC TCTTATGGCC 
CTAATACGCA CGGCACTGGA TATCAAAATA GCTAAAGGAG GTGCTGACAG GCAACAGCTG 
GACTCAGAGC TGCAGAAAGA TATACTTGCC ATTTGGCCAA ATCTCTCCCA GAAAATGATC 
GACTTACTTG TGCCAATGCC AAGAGCCTCT GATCTGACGG TGGGGAAGAT TTACGCTGCA 
ATGATGATCA TGGATTACTA TAAACAGAGC AAAGCGAAAA AACAAAGGCA GCAGCTGGAG 
GAGCAGAAAA ATGCACCTAT GTTTCAGAGA ATGGAGGCTT CTGCCCTCCC TGAGGAGATC 
TTATCCAATG CCAAAGCTTT ACCATACCTT CAGCAAGACA CTCTATCAGG CCTGGGTGGA 
TTTCCTTCAC TGAGTCCCTT GTCCCCAGAA GAAGTATTCC AGCCGCCATG CCTAGACGGG 
GAAGAGGAGG AAGACTTTGA AGAGCAGCTG TCTCTGGAGC CTGAGGTTAG AGAGTTTAGA 
AGGGAGCAGC TCCCTAACCG AGGCAGCTAC ATCCCTGTGG AGATGCTGGA ACGTCCAGCG 
TCTGTGAGAG CTTCTTCCAT GCCACGTCTG GCTGAGAATC CTCAGGTCCT GTCAGACGTA 
AGCTCCATGC GTCGCTCCTT CTCCACAACT GCAGAGAAGC GAGTGCGCAG CTCGTGGCTA 
GATGAGTTCA CTATGGACAG GAGTAGCACT GACTGTGCAT ACAGATACCA GAGGCGCAGT 
TATCATGCAT CCTTAAGACT GTCTGCACAT CGCCTGAATA CAGATACAGG TCACTGGTCA 
GATAATCACC GTTCTGGCGG AAGGGAGCGA GGAAGGTCCA AAGAGAGAAA ACACTTGCTG 
TCACCCGATC CTTCCCGCTG TAATTCTGAG GAAAGGAGCC CACAAGCTGC CTGTGAACAC 
CGAGATCACC AACTGTCCCC TGGGCATACT CCCAGTAGTG TACAGATTCT TGACTCTCTG 
AGTGAAAGTT GTACCCCTTC AGACACCAGC ACCCCAAGAA GAGGCCGTAG ACAGCTTCCT 
TCTGTGCCTC CAACTCCTAG ACCCCTTATC TCTTTCTCCT CGTTAATCCG CCGGCAGAAA 
CACAGCCCCC CTCCTCAAAA TAAAAATGAT ACTATCCTTC CTAGTGAGCC TGGTCTGACT 
GAGTCTTCCT GCTCCCTTGG GGGTCATCAG AGCTCCCAGT CCACACCACA GCATTATATC 
TCAGAGCCCT ACCTGGCACT GCATGAGGAG ATGCAAAGCT CAGACTGCCC AGCAGAGGAC 
ACACTCACCT TTGAGGTTGC TGTGGCTACT AGTCTGGGCC GCTCGAACAC CATTGGCTCT 
GCCCCTCTAC GTCATGGCTG GCAAATGCCC AATGGTCACT ACCGGCGTCG AAGGAAGGGA 
GGAATTCCAC CAGTGATACC CTCCAGAGAG TTAAGTGACA CAGAAGAGGA TGATCGATGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756842.1
CDS	77..6859
Translation	MLAPAGAGEQIGTGVQLQPGYRMMARFGEAVTGRLSSADGGSERSKTLQGSPAAPVGTQAAFKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIIRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPGDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGLVFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHKACYRNNSGMELDPMDPPHPCGVPRCPAGYECKEWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNSGTSALEVLRRATIKRSRTEAMNRDSTEDHCVDISSVGTPLARASIKSTKMDGASYFRHKEKLLRVSVRHMIKSQVFYWMVLGVVALNTFCVAIVHHNQTLWLSNVLYYAEFLFLGLFLVEMAFKMYGMGPRLYFHSSFNCFDCGVTIGSIFEVLWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMNSMKSIISLLFLLFLFIVVFALLGMQLYGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVRSGMWSSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNASCIEKERRKRHTLSIWEQRTSQLRRHIQMSSQEALQSEELAALNPHSSIYRKTKEAAEQNTEKEEVHQDAKLAIDPVEGPPEGEGSPSNRNLRENWRQKSRHGNSENTEPNGTTPEEAMRRRQSQRRSRHRRVRTEGREQCSNPNSTSASREGGLDKVSAEDGRTIPEEKSNGEAKEVKGVDDEPLKICISSRNCEDRNIRTLGCEGIALNDTPLQDVNSQEKDSSPPDLDCSEQALLGSIQSQESPNPTSCSLPDVSSSTERVTESTVITLPDTGMPTDCTVVQMSEGRDGEVNFGSSQQEKEEEEAEDGEERTIKRKEKLLESPKAIVPYSSMLIFSSTNPIRKVCHYIVNLRYFEMSILLVIAASSIALAAEDPVLTNSDRNKVLRYFDYVFTGVFTFEMIIKMIDQGLILHEGSYFRDLWNILDFVVVVGALIAFALANVLGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKETEKECIGSYIDHEKNKMEVKRREWKRHDFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDITEEDRGPSRSNRMEMSIFYVIYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPYTYELALKYLNIAFTMLFSLECILKIIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSSFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIRLEEDSHINRHNNFRSFFSSLMLLFRSATGEAWQEIMLSCLSDKGCEPDTSAASGHNENERCGTELAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRIWAEYDRAACGRIHYTEMYDMLTLMSPPLGLGKRCPNKVAYKRLVLMNMPVSEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKDILAIWPNLSQKMIDLLVPMPRASDLTVGKIYAAMMIMDYYKQSKAKKQRQQLEEQKNAPMFQRMEASALPEEILSNAKALPYLQQDTLSGLGGFPSLSPLSPEEVFQPPCLDGEEEEDFEEQLSLEPEVREFRREQLPNRGSYIPVEMLERPASVRASSMPRLAENPQVLSDVSSMRRSFSTTAEKRVRSSWLDEFTMDRSSTDCAYRYQRRSYHASLRLSAHRLNTDTGHWSDNHRSGGRERGRSKERKHLLSPDPSRCNSEERSPQAACEHRDHQLSPGHTPSSVQILDSLSESCTPSDTSTPRRGRRQLPSVPPTPRPLISFSSLIRRQKHSPPPQNKNDTILPSEPGLTESSCSLGGHQSSQSTPQHYISEPYLALHEEMQSSDCPAEDTLTFEVAVATSLGRSNTIGSAPLRHGWQMPNGHYRRRRKGGIPPVIPSRELSDTEEDDRC

Target ORF information:

RefSeq Version	XM_031900982.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calcium channel, voltage-dependent, R type, alpha 1E subunit (cacna1e), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031900982.1

ORF Insert Sequence:

ATGTTAGCGC CGGCAGGAGC CGGGGAGCAG ATCGGCACAG GAGTGCAGCT TCAACCCGGC 
TATAGGATGA TGGCTCGTTT CGGAGAGGCT GTGACTGGCA GGTTGAGCTC CGCAGATGGG 
GGCTCAGAGA GGAGCAAGAC CCTACAAGGT TCCCCAGCTG CACCTGTTGG GACCCAAGCA 
GCTTTCAAGC AATCCAAAGC CCAGAGGGCA AGGACCATGG CTTTATACAA CCCCATCCCT 
GTCCGACAGA ACTGCTTCAC CGTGAACAGG TCTCTCTTCA TTTTCGGAGA AGATAATATT 
ATCAGGAAAT ACGCCAAGAA GCTCATTGAT TGGCCTCCTT TTGAATACAT GATCCTTGCC 
ACAATTATTG CAAACTGCAT TGTCCTGGCT TTGGAGCAGC ATCTTCCAGG CGATGACAAG 
ACTCCAATGT CTCGCAGATT GGAAAAGACT GAGCCCTATT TCATTGGGAT ATTTTGCTTT 
GAAGCTGGAA TTAAAATTGT CGCTCTGGGA CTGGTTTTTC ACAAAGGTTC TTATCTTCGG 
AATGGCTGGA ATGTCATGGA CTTTATAGTA GTGCTCAGTG GCATCCTGGC CACAGCTGGA 
ACTCACTTCA ATACTCACGT GGACCTGCGT ACTTTGCGGG CAGTGCGTGT TCTCAGGCCG 
TTGAAGCTGG TCTCGGGGAT TCCCAGTTTA CAGATTGTGT TGAAGTCAAT CATGAAGGCG 
ATGGTCCCTC TGCTACAGAT TGGATTGTTG CTCTTCTTTG CCATCCTTAT GTTTGCTATC 
ATCGGCCTTG AGTTTTACAG TGGAAAGCTA CACAAGGCCT GTTACAGGAA CAACTCTGGA 
ATGGAGCTAG ACCCCATGGA TCCACCTCAC CCTTGTGGAG TGCCCCGCTG CCCAGCTGGG 
TATGAGTGCA AGGAATGGAT CGGACCCAAT GATGGAATAA CACAGTTTGA CAACATCCTA 
TTTGCGGTGC TGACAGTCTT CCAGTGTATA ACTATGGAGG GCTGGACCAC AGTGCTGTAC 
AATACCAATG ATGCCTTAGG AGCCACATGG AACTGGCTGT ACTTCATTCC CCTCATTATC 
ATCGGATCCT TCTTTGTCCT TAATCTTGTC CTTGGAGTGC TGTCCGGTGA ATTTGCCAAA 
GAGAGGGAAA GAGTGGAAAA CCGCCGGGCT TTCATGAAGT TGCGCAGGCA GCAGCAGATT 
GAGAGGGAGT TGAATGGCTA TCGGGCATGG ATAGATAAAG CTGAGGAAGT CATGTTGGCA 
GAAGAAAACA AAAATTCTGG GACCTCTGCT TTAGAAGTTC TTAGGCGAGC AACCATTAAA 
CGAAGTAGAA CAGAAGCCAT GAACCGGGAC TCTACTGAGG ACCATTGTGT TGACATATCC 
TCTGTTGGCA CCCCCCTTGC TAGAGCAAGC ATCAAAAGTA CCAAAATGGA TGGGGCATCA 
TATTTTCGGC ACAAGGAAAA ACTATTGCGT GTCTCCGTTC GTCACATGAT CAAATCCCAG 
GTTTTCTACT GGATGGTCCT GGGAGTGGTA GCACTTAACA CTTTCTGTGT GGCTATTGTC 
CATCACAACC AGACCCTGTG GCTCTCAAAT GTTCTTTACT ATGCAGAATT TCTGTTTCTT 
GGGTTATTTT TGGTGGAGAT GGCTTTTAAA ATGTATGGAA TGGGTCCACG TCTTTACTTT 
CACTCATCCT TCAACTGCTT TGATTGCGGG GTCACAATAG GTAGTATATT TGAAGTACTT 
TGGGCCATAT TTAGACCAGG AACTTCCTTT GGAATCAGTG TCTTAAGAGC TCTCCGACTT 
TTGAGAATCT TCAAAATAAC AAAATACTGG GCCTCACTAC GAAACCTTGT AGTATCATTG 
ATGAATTCTA TGAAATCCAT CATCAGCCTG CTCTTCCTTC TATTCCTCTT CATAGTTGTT 
TTTGCTCTCC TTGGAATGCA GTTATATGGT GGCAGATTCA ATTTTAATGA TGGCACTCCT 
TCTGCAAACT TTGATACATT CCCTGCAGCC ATCATGACTG TATTCCAGAT TCTGACAGGA 
GAGGATTGGA ATGAAGTGAT GTACAATGGT ATTCGCTCCC AAGGTGGTGT TCGATCCGGA 
ATGTGGTCTT CCATATACTT TATTGTCCTT ACCCTTTTTG GAAATTACAC ACTGCTAAAT 
GTATTTCTTG CCATTGCTGT GGACAACCTG GCTAATGCAC AGGAGTTAAC TAAGGATGAG 
CAAGAAGAAG AGGAAGCTTT CAATCAGAAG CATGCACTGC AAAAAGCGAA GGAGGTCAGC 
CCCATGTCTG CACCTAATGC ATCATGTATT GAGAAGGAGC GCAGAAAACG GCACACATTG 
TCTATATGGG AGCAGCGCAC AAGCCAGCTA AGGAGGCACA TACAGATGTC TAGTCAAGAA 
GCTCTCCAGA GTGAAGAACT GGCAGCATTG AATCCACACT CCAGTATCTA TCGCAAGACA 
AAGGAAGCTG CTGAACAAAA CACAGAAAAA GAAGAAGTGC ATCAAGACGC AAAACTGGCA 
ATAGACCCAG TTGAAGGACC ACCAGAAGGT GAAGGAAGCC CTTCTAATAG AAATCTCAGG 
GAAAACTGGC GCCAGAAGTC CAGGCATGGA AATTCTGAAA ACACTGAGCC AAATGGAACA 
ACTCCAGAAG AAGCAATGCG CCGCAGACAA AGTCAGAGAC GCAGCCGTCA TAGAAGAGTT 
AGAACTGAAG GTCGTGAGCA GTGTAGCAAT CCAAACAGTA CCTCAGCAAG CAGAGAAGGT 
GGTCTTGACA AAGTCTCTGC AGAGGATGGA AGAACAATTC CAGAAGAGAA GAGCAATGGA 
GAAGCCAAAG AAGTCAAAGG GGTTGATGAT GAGCCTCTAA AGATTTGTAT ATCTTCAAGA 
AATTGCGAGG ACAGAAATAT CAGGACTTTG GGTTGTGAGG GTATTGCCTT GAACGATACA 
CCCCTGCAGG ATGTGAACAG CCAAGAGAAG GACAGTAGCC CTCCTGATTT GGATTGCAGT 
GAGCAGGCTC TGTTGGGCAG CATTCAGAGC CAGGAAAGCC CTAATCCAAC CAGCTGCAGT 
TTGCCAGATG TGTCCAGCAG CACCGAGAGA GTCACAGAGA GCACCGTCAT TACCTTACCA 
GATACTGGAA TGCCCACCGA CTGCACCGTG GTACAGATGA GTGAAGGCAG GGATGGAGAA 
GTCAACTTTG GGTCTTCGCA GCAAGAAAAA GAAGAGGAGG AAGCTGAGGA TGGAGAAGAA 
AGAACTATAA AAAGAAAAGA GAAATTGTTG GAGTCCCCCA AAGCCATTGT ACCGTACAGT 
TCAATGCTCA TCTTCAGCTC CACCAATCCC ATAAGAAAAG TATGTCATTA CATTGTTAAC 
CTACGATACT TTGAGATGAG CATCCTGCTG GTAATAGCAG CAAGTAGTAT AGCCTTAGCA 
GCGGAGGACC CTGTACTGAC CAACTCGGAC CGCAACAAAG TTCTTCGCTA TTTTGACTAT 
GTTTTCACAG GGGTCTTCAC TTTTGAGATG ATTATAAAGA TGATTGACCA AGGTTTGATT 
TTACACGAGG GCTCCTACTT TCGTGACCTA TGGAACATCT TGGATTTTGT TGTTGTTGTG 
GGAGCCTTAA TTGCGTTTGC CTTAGCGAAT GTTTTGGGGA CTAACAAAGG CCGGGACATC 
AAAACCATTA AGTCTCTACG TGTGTTACGG GTGCTGCGTC CACTAAAAAC AATCAAACGC 
CTGCCAAAAC TGAAGGCAGT CTTTGATTGT GTGGTTAACT CTTTAAAGAA TGTCTTTAAT 
ATTCTCATCG TATACAAACT CTTCATGTTC ATATTTGCTG TAATCGCTGT CCAGCTGTTT 
AAGGGCAAGT TCTTCTATTG TACAGACAGC TCAAAGGAGA CAGAGAAAGA GTGCATAGGG 
AGTTACATCG ATCACGAAAA GAACAAGATG GAGGTGAAGC GGAGAGAGTG GAAAAGACAT 
GATTTCCATT ATGACAACAT AATATGGGCT CTTCTTACCC TCTTTACTGT ATCAACAGGG 
GAAGGCTGGC CTCAGGTTCT GCAGCACTCT GTTGATATAA CTGAAGAAGA TAGGGGTCCA 
AGCCGAAGCA ACCGTATGGA GATGTCCATT TTCTATGTTA TCTACTTTGT GGTCTTCCCC 
TTCTTCTTTG TAAATATATT TGTGGCTCTG ATTATCATCA CATTCCAGGA GCAAGGAGAC 
AAGATGATGG AGGAATGTTC CCTAGAAAAG AATGAGAGAG CCTGCATAGA CTTTGCAATA 
AGTGCTAAGC CACTTACACG ATATATGCCA CAGAACAGGC ACACATTCCA GTACCGGGTC 
TGGCACTTCG TGGTCTCCCC ATCATTTGAA TACACTATCA TGGCTATGAT TGCACTAAAC 
ACAGTGGTAC TCATGATGAA GTATTATTCT GCGCCATACA CATATGAGCT TGCATTAAAG 
TATCTCAATA TTGCATTCAC AATGCTATTT TCTCTGGAGT GCATCCTGAA AATAATTGCA 
TTTGGGTTTT TGAATTACTT TCGGGATACT TGGAACATTT TTGACTTCAT TACTGTAATT 
GGTAGCATCA CGGAAATTAT TCTAACAGAT AGTAAGTTGG TGAACACCAG CAGCTTTAAT 
ATGAGCTTTC TGAAGCTGTT CCGAGCGGCG CGACTTATTA AACTTCTCCG GCAAGGGTAC 
ACCATCCGGA TCCTGCTGTG GACTTTTGTT CAGTCCTTTA AGGCACTTCC TTATGTGTGT 
CTTCTCATTG CAATGTTGTT CTTCATCTAT GCCATTATTG GGATGCAGGT GTTTGGCAAT 
ATCCGCCTGG AAGAGGACAG TCATATTAAT AGGCACAACA ATTTTAGAAG CTTCTTTAGT 
TCTCTTATGT TACTCTTCAG GAGTGCCACA GGAGAAGCGT GGCAGGAGAT CATGCTTTCA 
TGCCTTAGTG ATAAGGGTTG TGAGCCAGAC ACCAGTGCAG CCTCTGGACA CAACGAGAAT 
GAGCGCTGTG GAACTGAACT TGCATATGTT TATTTTGTTT CATTCATTTT CTTCTGCTCC 
TTCCTCATGC TAAATCTGTT TGTGGCTGTC ATTATGGACA ACTTTGAGTA CTTGACACGG 
GACTCTTCCA TTTTGGGACC TCATCACTTG GATGAGTTTG TTCGGATATG GGCTGAATAT 
GATCGAGCAG CATGTGGCCG TATCCATTAC ACAGAGATGT ATGACATGCT GACTCTCATG 
TCACCACCTC TAGGCCTTGG AAAGAGATGC CCTAACAAAG TGGCTTATAA GAGATTGGTC 
CTGATGAATA TGCCAGTGTC GGAGGACATG ACGGTTCATT TCACATCAAC TCTTATGGCC 
CTAATACGCA CGGCACTGGA TATCAAAATA GCTAAAGGAG GTGCTGACAG GCAACAGCTG 
GACTCAGAGC TGCAGAAAGA TATACTTGCC ATTTGGCCAA ATCTCTCCCA GAAAATGATC 
GACTTACTTG TGCCAATGCC AAGAGCCTCT GATCTGACGG TGGGGAAGAT TTACGCTGCA 
ATGATGATCA TGGATTACTA TAAACAGAGC AAAGCGAAAA AACAAAGGCA GCAGCTGGAG 
GAGCAGAAAA ATGCACCTAT GTTTCAGAGA ATGGAGGCTT CTGCCCTCCC TGAGGAGATC 
TTATCCAATG CCAAAGCTTT ACCATACCTT CAGCAAGACA CTCTATCAGG CCTGGGTGGA 
TTTCCTTCAC TGAGTCCCTT GTCCCCAGAA GAAGTATTCC AGCCGCCATG CCTAGACGGG 
GAAGAGGAGG AAGACTTTGA AGAGCAGCTG TCTCTGGAGC CTGAGGTTAG AGAGTTTAGA 
AGGGAGCAGC TCCCTAACCG AGGCAGCTAC ATCCCTGTGG AGATGCTGGA ACGTCCAGCG 
TCTGTGAGAG CTTCTTCCAT GCCACGTCTG GCTGAGAATC CTCAGGTCCT GTCAGACGTA 
AGCTCCATGC GTCGCTCCTT CTCCACAACT GCAGAGAAGC GAGTGCGCAG CTCGTGGCTA 
GATGAGTTCA CTATGGACAG GAGTAGCACT GACTGTGCAT ACAGATACCA GAGGCGCAGT 
TATCATGCAT CCTTAAGACT GTCTGCACAT CGCCTGAATA CAGATACAGG TCACTGGTCA 
GATAATCACC GTTCTGGCGG AAGGGAGCGA GGAAGGTCCA AAGAGAGAAA ACACTTGCTG 
TCACCCGATC CTTCCCGCTG TAATTCTGAG GAAAGGAGCC CACAAGCTGC CTGTGAACAC 
CGAGATCACC AACTGTCCCC TGGGCATACT CCCAGTAGTG TACAGATTCT TGACTCTCTG 
AGTGAAAGTT GTACCCCTTC AGACACCAGC ACCCCAAGAA GAGGCCGTAG ACAGCTTCCT 
TCTGTGCCTC CAACTCCTAG ACCCCTTATC TCTTTCTCCT CGTTAATCCG CCGGCAGAAA 
CACAGCCCCC CTCCTCAAAA TAAAAATGAT ACTATCCTTC CTAGTGAGCC TGGTCTGACT 
GAGTCTTCCT GCTCCCTTGG GGGTCATCAG AGCTCCCAGT CCACACCACA GCATTATATC 
TCAGAGCCCT ACCTGGCACT GCATGAGGAG ATGCAAAGCT CAGACTGCCC AGCAGAGGAC 
ACACTCACCT TTGAGGTTGC TGTGGCTACT AGTCTGGGCC GCTCGAACAC CATTGGCTCT 
GCCCCTCTAC GTCATGGCTG GCAAATGCCC AATGGTCACT ACCGGCGTCG AAGGAAGGGA 
GGAATTCCAC CAGTGATACC CTCCAGAGAG TTAAGTGACA CAGAAGAGGA TGATCGATGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1e ( XM_031900983.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756843.1 Xenopus tropicalis calcium channel, voltage-dependent, R type, alpha 1E subunit (cacna1e), transcript variant X6, mRNA.

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CloneID	OXa57234
Clone ID Related Accession (Same CDS sequence)	XM_031900983.1
Accession Version	XM_031900983.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTATTAA AGATGCTGGG ATTAAGCTCC TGTTCAACCA GTGCTCATAC ACTCAATGAG 
CCTGACCAGT CAAGCACACT AGCCATACCA GCATTGGATG CCGACACTCA GAGATATGTC 
TTGGCCCTTC CAGAAGCAGA GACATGGCGC AAGCATCGAT TGGGCCTGAC ACAGGCTACT 
CACTCTTGCA ATCTTCTAGA ACCTGAGACA CTTTCTGAAA CCCTAGTTTC ACGCACTGCA 
AGTTACGATG CCTTGTATGA CCTGCGAGCA AGAGAAGCGG AGTCTAACTT CCCATTTGAA 
GGCAGCAGCA TGTTTGAGGT GACGTCTGGG ATGGATTCTG AGTTTCCTTT AAGCCCCCAT 
GTTATAAAGC GAAGGAGAGG AGGCCTGATA GAACAAAAGG ATATCATCAA GGCCCACGAA 
GCTCATAAGA TTCAGAGTAC TCCCCAGGCT CGACGGAAGG AATGGGAGAT GGCTCGTTTC 
GGAGAGGCTG TGACTGGCAG GTTGAGCTCC GCAGATGGGG GCTCAGAGAG GAGCAAGACC 
CTACAAGGTT CCCCAGCTGC ACCTGTTGGG ACCCAAGCAG CTTTCAAGCA ATCCAAAGCC 
CAGAGGGCAA GGACCATGGC TTTATACAAC CCCATCCCTG TCCGACAGAA CTGCTTCACC 
GTGAACAGGT CTCTCTTCAT TTTCGGAGAA GATAATATTA TCAGGAAATA CGCCAAGAAG 
CTCATTGATT GGCCTCCTTT TGAATACATG ATCCTTGCCA CAATTATTGC AAACTGCATT 
GTCCTGGCTT TGGAGCAGCA TCTTCCAGGC GATGACAAGA CTCCAATGTC TCGCAGATTG 
GAAAAGACTG AGCCCTATTT CATTGGGATA TTTTGCTTTG AAGCTGGAAT TAAAATTGTC 
GCTCTGGGAC TGGTTTTTCA CAAAGGTTCT TATCTTCGGA ATGGCTGGAA TGTCATGGAC 
TTTATAGTAG TGCTCAGTGG CATCCTGGCC ACAGCTGGAA CTCACTTCAA TACTCACGTG 
GACCTGCGTA CTTTGCGGGC AGTGCGTGTT CTCAGGCCGT TGAAGCTGGT CTCGGGGATT 
CCCAGTTTAC AGATTGTGTT GAAGTCAATC ATGAAGGCGA TGGTCCCTCT GCTACAGATT 
GGATTGTTGC TCTTCTTTGC CATCCTTATG TTTGCTATCA TCGGCCTTGA GTTTTACAGT 
GGAAAGCTAC ACAAGGCCTG TTACAGGAAC AACTCTGGAA TGGAGCTAGA CCCCATGGAT 
CCACCTCACC CTTGTGGAGT GCCCCGCTGC CCAGCTGGGT ATGAGTGCAA GGAATGGATC 
GGACCCAATG ATGGAATAAC ACAGTTTGAC AACATCCTAT TTGCGGTGCT GACAGTCTTC 
CAGTGTATAA CTATGGAGGG CTGGACCACA GTGCTGTACA ATACCAATGA TGCCTTAGGA 
GCCACATGGA ACTGGCTGTA CTTCATTCCC CTCATTATCA TCGGATCCTT CTTTGTCCTT 
AATCTTGTCC TTGGAGTGCT GTCCGGTGAA TTTGCCAAAG AGAGGGAAAG AGTGGAAAAC 
CGCCGGGCTT TCATGAAGTT GCGCAGGCAG CAGCAGATTG AGAGGGAGTT GAATGGCTAT 
CGGGCATGGA TAGATAAAGC TGAGGAAGTC ATGTTGGCAG AAGAAAACAA AAATTCTGGG 
ACCTCTGCTT TAGAAGTTCT TAGGCGAGCA ACCATTAAAC GAAGTAGAAC AGAAGCCATG 
AACCGGGACT CTACTGAGGA CCATTGTGTT GACATATCCT CTGTTGGCAC CCCCCTTGCT 
AGAGCAAGCA TCAAAAGTAC CAAAATGGAT GGGGCATCAT ATTTTCGGCA CAAGGAAAAA 
CTATTGCGTG TCTCCGTTCG TCACATGATC AAATCCCAGG TTTTCTACTG GATGGTCCTG 
GGAGTGGTAG CACTTAACAC TTTCTGTGTG GCTATTGTCC ATCACAACCA GACCCTGTGG 
CTCTCAAATG TTCTTTACTA TGCAGAATTT CTGTTTCTTG GGTTATTTTT GGTGGAGATG 
GCTTTTAAAA TGTATGGAAT GGGTCCACGT CTTTACTTTC ACTCATCCTT CAACTGCTTT 
GATTGCGGGG TCACAATAGG TAGTATATTT GAAGTACTTT GGGCCATATT TAGACCAGGA 
ACTTCCTTTG GAATCAGTGT CTTAAGAGCT CTCCGACTTT TGAGAATCTT CAAAATAACA 
AAATACTGGG CCTCACTACG AAACCTTGTA GTATCATTGA TGAATTCTAT GAAATCCATC 
ATCAGCCTGC TCTTCCTTCT ATTCCTCTTC ATAGTTGTTT TTGCTCTCCT TGGAATGCAG 
TTATATGGTG GCAGATTCAA TTTTAATGAT GGCACTCCTT CTGCAAACTT TGATACATTC 
CCTGCAGCCA TCATGACTGT ATTCCAGATT CTGACAGGAG AGGATTGGAA TGAAGTGATG 
TACAATGGTA TTCGCTCCCA AGGTGGTGTT CGATCCGGAA TGTGGTCTTC CATATACTTT 
ATTGTCCTTA CCCTTTTTGG AAATTACACA CTGCTAAATG TATTTCTTGC CATTGCTGTG 
GACAACCTGG CTAATGCACA GGAGTTAACT AAGGATGAGC AAGAAGAAGA GGAAGCTTTC 
AATCAGAAGC ATGCACTGCA AAAAGCGAAG GAGGTCAGCC CCATGTCTGC ACCTAATGCA 
TCATGTATTG AGAAGGAGCG CAGAAAACGG CACACATTGT CTATATGGGA GCAGCGCACA 
AGCCAGCTAA GGAGGCACAT ACAGATGTCT AGTCAAGAAG CTCTCCAGAG TGAAGAACTG 
GCAGCATTGA ATCCACACTC CAGTATCTAT CGCAAGACAA AGGAAGCTGC TGAACAAAAC 
ACAGAAAAAG AAGAAGTGCA TCAAGACGCA AAACTGGCAA TAGACCCAGT TGAAGGACCA 
CCAGAAGGTG AAGGAAGCCC TTCTAATAGA AATCTCAGGG AAAACTGGCG CCAGAAGTCC 
AGGCATGGAA ATTCTGAAAA CACTGAGCCA AATGGAACAA CTCCAGAAGA AGCAATGCGC 
CGCAGACAAA GTCAGAGACG CAGCCGTCAT AGAAGAGTTA GAACTGAAGG TCGTGAGCAG 
TGTAGCAATC CAAACAGTAC CTCAGCAAGC AGAGAAGGTG GTCTTGACAA AGTCTCTGCA 
GAGGATGGAA GAACAATTCC AGAAGAGAAG AGCAATGGAG AAGCCAAAGA AGTCAAAGGG 
GTTGATGATG AGCCTCTAAA GATTTGTATA TCTTCAAGAA ATTGCGAGGA CAGAAATATC 
AGGACTTTGG GTTGTGAGGG TATTGCCTTG AACGATACAC CCCTGCAGGA TGTGAACAGC 
CAAGAGAAGG ACAGTAGCCC TCCTGATTTG GATTGCAGTG AGCAGGCTCT GTTGGGCAGC 
ATTCAGAGCC AGGAAAGCCC TAATCCAACC AGCTGCAGTT TGCCAGATGT GTCCAGCAGC 
ACCGAGAGAG TCACAGAGAG CACCGTCATT ACCTTACCAG ATACTGGAAT GCCCACCGAC 
TGCACCGTGG TACAGATGAG TGAAGGCAGG GATGGAGAAG TCAACTTTGG GTCTTCGCAG 
CAAGAAAAAG AAGAGGAGGA AGCTGAGGAT GGAGAAGAAA GAACTATAAA AAGAAAAGAG 
AAATTGTTGG AGTCCCCCAA AGCCATTGTA CCGTACAGTT CAATGCTCAT CTTCAGCTCC 
ACCAATCCCA TAAGAAAAGT ATGTCATTAC ATTGTTAACC TACGATACTT TGAGATGAGC 
ATCCTGCTGG TAATAGCAGC AAGTAGTATA GCCTTAGCAG CGGAGGACCC TGTACTGACC 
AACTCGGACC GCAACAAAGT TCTTCGCTAT TTTGACTATG TTTTCACAGG GGTCTTCACT 
TTTGAGATGA TTATAAAGAT GATTGACCAA GGTTTGATTT TACACGAGGG CTCCTACTTT 
CGTGACCTAT GGAACATCTT GGATTTTGTT GTTGTTGTGG GAGCCTTAAT TGCGTTTGCC 
TTAGCGAATG TTTTGGGGAC TAACAAAGGC CGGGACATCA AAACCATTAA GTCTCTACGT 
GTGTTACGGG TGCTGCGTCC ACTAAAAACA ATCAAACGCC TGCCAAAACT GAAGGCAGTC 
TTTGATTGTG TGGTTAACTC TTTAAAGAAT GTCTTTAATA TTCTCATCGT ATACAAACTC 
TTCATGTTCA TATTTGCTGT AATCGCTGTC CAGCTGTTTA AGGGCAAGTT CTTCTATTGT 
ACAGACAGCT CAAAGGAGAC AGAGAAAGAG TGCATAGGGA GTTACATCGA TCACGAAAAG 
AACAAGATGG AGGTGAAGCG GAGAGAGTGG AAAAGACATG ATTTCCATTA TGACAACATA 
ATATGGGCTC TTCTTACCCT CTTTACTGTA TCAACAGGGG AAGGCTGGCC TCAGGTTCTG 
CAGCACTCTG TTGATATAAC TGAAGAAGAT AGGGGTCCAA GCCGAAGCAA CCGTATGGAG 
ATGTCCATTT TCTATGTTAT CTACTTTGTG GTCTTCCCCT TCTTCTTTGT AAATATATTT 
GTGGCTCTGA TTATCATCAC ATTCCAGGAG CAAGGAGACA AGATGATGGA GGAATGTTCC 
CTAGAAAAGA ATGAGAGAGC CTGCATAGAC TTTGCAATAA GTGCTAAGCC ACTTACACGA 
TATATGCCAC AGAACAGGCA CACATTCCAG TACCGGGTCT GGCACTTCGT GGTCTCCCCA 
TCATTTGAAT ACACTATCAT GGCTATGATT GCACTAAACA CAGTGGTACT CATGATGAAG 
TATTATTCTG CGCCATACAC ATATGAGCTT GCATTAAAGT ATCTCAATAT TGCATTCACA 
ATGCTATTTT CTCTGGAGTG CATCCTGAAA ATAATTGCAT TTGGGTTTTT GAATTACTTT 
CGGGATACTT GGAACATTTT TGACTTCATT ACTGTAATTG GTAGCATCAC GGAAATTATT 
CTAACAGATA GTAAGTTGGT GAACACCAGC AGCTTTAATA TGAGCTTTCT GAAGCTGTTC 
CGAGCGGCGC GACTTATTAA ACTTCTCCGG CAAGGGTACA CCATCCGGAT CCTGCTGTGG 
ACTTTTGTTC AGTCCTTTAA GGCACTTCCT TATGTGTGTC TTCTCATTGC AATGTTGTTC 
TTCATCTATG CCATTATTGG GATGCAGGTG TTTGGCAATA TCCGCCTGGA AGAGGACAGT 
CATATTAATA GGCACAACAA TTTTAGAAGC TTCTTTAGTT CTCTTATGTT ACTCTTCAGG 
AGTGCCACAG GAGAAGCGTG GCAGGAGATC ATGCTTTCAT GCCTTAGTGA TAAGGGTTGT 
GAGCCAGACA CCAGTGCAGC CTCTGGACAC AACGAGAATG AGCGCTGTGG AACTGAACTT 
GCATATGTTT ATTTTGTTTC ATTCATTTTC TTCTGCTCCT TCCTCATGCT AAATCTGTTT 
GTGGCTGTCA TTATGGACAA CTTTGAGTAC TTGACACGGG ACTCTTCCAT TTTGGGACCT 
CATCACTTGG ATGAGTTTGT TCGGATATGG GCTGAATATG ATCGAGCAGC ATGTGGCAGG 
ATCCCCTATA AGGATATGTA CAAATTAGTC CGAGTCATCT CGCCTCCGCT CGGCCTAGGA 
GAGAACTGCC CATACAGGGT GGCATGCAAG AGATTGGTCC TGATGAATAT GCCAGTGTCG 
GAGGACATGA CGGTTCATTT CACATCAACT CTTATGGCCC TAATACGCAC GGCACTGGAT 
ATCAAAATAG CTAAAGGAGG TGCTGACAGG CAACAGCTGG ACTCAGAGCT GCAGAAAGAT 
ATACTTGCCA TTTGGCCAAA TCTCTCCCAG AAAATGATCG ACTTACTTGT GCCAATGCCA 
AGAGCCTCTG ATCTGACGGT GGGGAAGATT TACGCTGCAA TGATGATCAT GGATTACTAT 
AAACAGAGCA AAGCGAAAAA ACAAAGGCAG CAGCTGGAGG AGCAGAAAAA TGCACCTATG 
TTTCAGAGAA TGGAGGCTTC TGCCCTCCCT GAGGAGATCT TATCCAATGC CAAAGCTTTA 
CCATACCTTC AGCAAGACAC TCTATCAGGC CTGGGTGGAT TTCCTTCACT GAGTCCCTTG 
TCCCCAGAAG AAGTATTCCA GCCGCCATGC CTAGACGGGG AAGAGGAGGA AGACTTTGAA 
GAGCAGCTGT CTCTGGTCCT GTCAGACGTA AGCTCCATGC GTCGCTCCTT CTCCACAACT 
GCAGAGAAGC GAGTGCGCAG CTCGTGGCTA GATGAGTTCA CTATGGACAG GAGTAGCACT 
GACTGTGCAT ACAGATACCA GAGGCGCAGT TATCATGCAT CCTTAAGACT GTCTGCACAT 
CGCCTGAATA CAGATACAGG TCACTGGTCA GATAATCACC GTTCTGGCGG AAGGGAGCGA 
GGAAGGTCCA AAGAGAGAAA ACACTTGCTG TCACCCGATC CTTCCCGCTG TAATTCTGAG 
GAAAGGAGCC CACAAGCTGC CTGTGAACAC CGAGATCACC AACTGTCCCC TGGGCATACT 
CCCAGTAGTG TACAGATTCT TGACTCTCTG AGTGAAAGTT GTACCCCTTC AGACACCAGC 
ACCCCAAGAA GAGGCCGTAG ACAGCTTCCT TCTGTGCCTC CAACTCCTAG ACCCCTTATC 
TCTTTCTCCT CGTTAATCCG CCGGCAGAAA CACAGCCCCC CTCCTCAAAA TAAAAATGAT 
ACTATCCTTC CTAGTGAGCC TGGTCTGACT GAGTCTTCCT GCTCCCTTGG GGGTCATCAG 
AGCTCCCAGT CCACACCACA GCATTATATC TCAGAGCCCT ACCTGGCACT GCATGAGGAG 
ATGCAAAGCT CAGACTGCCC AGCAGAGGAC ACACTCACCT TTGAGGTTGC TGTGGCTACT 
AGTCTGGGCC GCTCGAACAC CATTGGCTCT GCCCCTCTAC GTCATGGCTG GCAAATGCCC 
AATGGTCACT ACCGGCGTCG AAGGAAGGGA GGAATTCCAC CAGTGATACC CTCCAGAGAG 
TTAAGTGACA CAGAAGAGGA TGATCGATGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756843.1
CDS	285..7337
Translation	MVLKMLGLSSCSTSAHTLNEPDQSSTLAIPALDADTQRYVLALPEAETWRKHRLGLTQATHSCNLLEPETLSETLVSRTASYDALYDLRAREAESNFPFEGSSMFEVTSGMDSEFPLSPHVIKRRRGGLIEQKDIIKAHEAHKIQSTPQARRKEWEMARFGEAVTGRLSSADGGSERSKTLQGSPAAPVGTQAAFKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIIRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPGDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGLVFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHKACYRNNSGMELDPMDPPHPCGVPRCPAGYECKEWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNSGTSALEVLRRATIKRSRTEAMNRDSTEDHCVDISSVGTPLARASIKSTKMDGASYFRHKEKLLRVSVRHMIKSQVFYWMVLGVVALNTFCVAIVHHNQTLWLSNVLYYAEFLFLGLFLVEMAFKMYGMGPRLYFHSSFNCFDCGVTIGSIFEVLWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMNSMKSIISLLFLLFLFIVVFALLGMQLYGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVRSGMWSSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNASCIEKERRKRHTLSIWEQRTSQLRRHIQMSSQEALQSEELAALNPHSSIYRKTKEAAEQNTEKEEVHQDAKLAIDPVEGPPEGEGSPSNRNLRENWRQKSRHGNSENTEPNGTTPEEAMRRRQSQRRSRHRRVRTEGREQCSNPNSTSASREGGLDKVSAEDGRTIPEEKSNGEAKEVKGVDDEPLKICISSRNCEDRNIRTLGCEGIALNDTPLQDVNSQEKDSSPPDLDCSEQALLGSIQSQESPNPTSCSLPDVSSSTERVTESTVITLPDTGMPTDCTVVQMSEGRDGEVNFGSSQQEKEEEEAEDGEERTIKRKEKLLESPKAIVPYSSMLIFSSTNPIRKVCHYIVNLRYFEMSILLVIAASSIALAAEDPVLTNSDRNKVLRYFDYVFTGVFTFEMIIKMIDQGLILHEGSYFRDLWNILDFVVVVGALIAFALANVLGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKETEKECIGSYIDHEKNKMEVKRREWKRHDFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDITEEDRGPSRSNRMEMSIFYVIYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPYTYELALKYLNIAFTMLFSLECILKIIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSSFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIRLEEDSHINRHNNFRSFFSSLMLLFRSATGEAWQEIMLSCLSDKGCEPDTSAASGHNENERCGTELAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRIWAEYDRAACGRIPYKDMYKLVRVISPPLGLGENCPYRVACKRLVLMNMPVSEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKDILAIWPNLSQKMIDLLVPMPRASDLTVGKIYAAMMIMDYYKQSKAKKQRQQLEEQKNAPMFQRMEASALPEEILSNAKALPYLQQDTLSGLGGFPSLSPLSPEEVFQPPCLDGEEEEDFEEQLSLVLSDVSSMRRSFSTTAEKRVRSSWLDEFTMDRSSTDCAYRYQRRSYHASLRLSAHRLNTDTGHWSDNHRSGGRERGRSKERKHLLSPDPSRCNSEERSPQAACEHRDHQLSPGHTPSSVQILDSLSESCTPSDTSTPRRGRRQLPSVPPTPRPLISFSSLIRRQKHSPPPQNKNDTILPSEPGLTESSCSLGGHQSSQSTPQHYISEPYLALHEEMQSSDCPAEDTLTFEVAVATSLGRSNTIGSAPLRHGWQMPNGHYRRRRKGGIPPVIPSRELSDTEEDDRC

Target ORF information:

RefSeq Version	XM_031900983.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calcium channel, voltage-dependent, R type, alpha 1E subunit (cacna1e), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031900983.1

ORF Insert Sequence:

ATGGTATTAA AGATGCTGGG ATTAAGCTCC TGTTCAACCA GTGCTCATAC ACTCAATGAG 
CCTGACCAGT CAAGCACACT AGCCATACCA GCATTGGATG CCGACACTCA GAGATATGTC 
TTGGCCCTTC CAGAAGCAGA GACATGGCGC AAGCATCGAT TGGGCCTGAC ACAGGCTACT 
CACTCTTGCA ATCTTCTAGA ACCTGAGACA CTTTCTGAAA CCCTAGTTTC ACGCACTGCA 
AGTTACGATG CCTTGTATGA CCTGCGAGCA AGAGAAGCGG AGTCTAACTT CCCATTTGAA 
GGCAGCAGCA TGTTTGAGGT GACGTCTGGG ATGGATTCTG AGTTTCCTTT AAGCCCCCAT 
GTTATAAAGC GAAGGAGAGG AGGCCTGATA GAACAAAAGG ATATCATCAA GGCCCACGAA 
GCTCATAAGA TTCAGAGTAC TCCCCAGGCT CGACGGAAGG AATGGGAGAT GGCTCGTTTC 
GGAGAGGCTG TGACTGGCAG GTTGAGCTCC GCAGATGGGG GCTCAGAGAG GAGCAAGACC 
CTACAAGGTT CCCCAGCTGC ACCTGTTGGG ACCCAAGCAG CTTTCAAGCA ATCCAAAGCC 
CAGAGGGCAA GGACCATGGC TTTATACAAC CCCATCCCTG TCCGACAGAA CTGCTTCACC 
GTGAACAGGT CTCTCTTCAT TTTCGGAGAA GATAATATTA TCAGGAAATA CGCCAAGAAG 
CTCATTGATT GGCCTCCTTT TGAATACATG ATCCTTGCCA CAATTATTGC AAACTGCATT 
GTCCTGGCTT TGGAGCAGCA TCTTCCAGGC GATGACAAGA CTCCAATGTC TCGCAGATTG 
GAAAAGACTG AGCCCTATTT CATTGGGATA TTTTGCTTTG AAGCTGGAAT TAAAATTGTC 
GCTCTGGGAC TGGTTTTTCA CAAAGGTTCT TATCTTCGGA ATGGCTGGAA TGTCATGGAC 
TTTATAGTAG TGCTCAGTGG CATCCTGGCC ACAGCTGGAA CTCACTTCAA TACTCACGTG 
GACCTGCGTA CTTTGCGGGC AGTGCGTGTT CTCAGGCCGT TGAAGCTGGT CTCGGGGATT 
CCCAGTTTAC AGATTGTGTT GAAGTCAATC ATGAAGGCGA TGGTCCCTCT GCTACAGATT 
GGATTGTTGC TCTTCTTTGC CATCCTTATG TTTGCTATCA TCGGCCTTGA GTTTTACAGT 
GGAAAGCTAC ACAAGGCCTG TTACAGGAAC AACTCTGGAA TGGAGCTAGA CCCCATGGAT 
CCACCTCACC CTTGTGGAGT GCCCCGCTGC CCAGCTGGGT ATGAGTGCAA GGAATGGATC 
GGACCCAATG ATGGAATAAC ACAGTTTGAC AACATCCTAT TTGCGGTGCT GACAGTCTTC 
CAGTGTATAA CTATGGAGGG CTGGACCACA GTGCTGTACA ATACCAATGA TGCCTTAGGA 
GCCACATGGA ACTGGCTGTA CTTCATTCCC CTCATTATCA TCGGATCCTT CTTTGTCCTT 
AATCTTGTCC TTGGAGTGCT GTCCGGTGAA TTTGCCAAAG AGAGGGAAAG AGTGGAAAAC 
CGCCGGGCTT TCATGAAGTT GCGCAGGCAG CAGCAGATTG AGAGGGAGTT GAATGGCTAT 
CGGGCATGGA TAGATAAAGC TGAGGAAGTC ATGTTGGCAG AAGAAAACAA AAATTCTGGG 
ACCTCTGCTT TAGAAGTTCT TAGGCGAGCA ACCATTAAAC GAAGTAGAAC AGAAGCCATG 
AACCGGGACT CTACTGAGGA CCATTGTGTT GACATATCCT CTGTTGGCAC CCCCCTTGCT 
AGAGCAAGCA TCAAAAGTAC CAAAATGGAT GGGGCATCAT ATTTTCGGCA CAAGGAAAAA 
CTATTGCGTG TCTCCGTTCG TCACATGATC AAATCCCAGG TTTTCTACTG GATGGTCCTG 
GGAGTGGTAG CACTTAACAC TTTCTGTGTG GCTATTGTCC ATCACAACCA GACCCTGTGG 
CTCTCAAATG TTCTTTACTA TGCAGAATTT CTGTTTCTTG GGTTATTTTT GGTGGAGATG 
GCTTTTAAAA TGTATGGAAT GGGTCCACGT CTTTACTTTC ACTCATCCTT CAACTGCTTT 
GATTGCGGGG TCACAATAGG TAGTATATTT GAAGTACTTT GGGCCATATT TAGACCAGGA 
ACTTCCTTTG GAATCAGTGT CTTAAGAGCT CTCCGACTTT TGAGAATCTT CAAAATAACA 
AAATACTGGG CCTCACTACG AAACCTTGTA GTATCATTGA TGAATTCTAT GAAATCCATC 
ATCAGCCTGC TCTTCCTTCT ATTCCTCTTC ATAGTTGTTT TTGCTCTCCT TGGAATGCAG 
TTATATGGTG GCAGATTCAA TTTTAATGAT GGCACTCCTT CTGCAAACTT TGATACATTC 
CCTGCAGCCA TCATGACTGT ATTCCAGATT CTGACAGGAG AGGATTGGAA TGAAGTGATG 
TACAATGGTA TTCGCTCCCA AGGTGGTGTT CGATCCGGAA TGTGGTCTTC CATATACTTT 
ATTGTCCTTA CCCTTTTTGG AAATTACACA CTGCTAAATG TATTTCTTGC CATTGCTGTG 
GACAACCTGG CTAATGCACA GGAGTTAACT AAGGATGAGC AAGAAGAAGA GGAAGCTTTC 
AATCAGAAGC ATGCACTGCA AAAAGCGAAG GAGGTCAGCC CCATGTCTGC ACCTAATGCA 
TCATGTATTG AGAAGGAGCG CAGAAAACGG CACACATTGT CTATATGGGA GCAGCGCACA 
AGCCAGCTAA GGAGGCACAT ACAGATGTCT AGTCAAGAAG CTCTCCAGAG TGAAGAACTG 
GCAGCATTGA ATCCACACTC CAGTATCTAT CGCAAGACAA AGGAAGCTGC TGAACAAAAC 
ACAGAAAAAG AAGAAGTGCA TCAAGACGCA AAACTGGCAA TAGACCCAGT TGAAGGACCA 
CCAGAAGGTG AAGGAAGCCC TTCTAATAGA AATCTCAGGG AAAACTGGCG CCAGAAGTCC 
AGGCATGGAA ATTCTGAAAA CACTGAGCCA AATGGAACAA CTCCAGAAGA AGCAATGCGC 
CGCAGACAAA GTCAGAGACG CAGCCGTCAT AGAAGAGTTA GAACTGAAGG TCGTGAGCAG 
TGTAGCAATC CAAACAGTAC CTCAGCAAGC AGAGAAGGTG GTCTTGACAA AGTCTCTGCA 
GAGGATGGAA GAACAATTCC AGAAGAGAAG AGCAATGGAG AAGCCAAAGA AGTCAAAGGG 
GTTGATGATG AGCCTCTAAA GATTTGTATA TCTTCAAGAA ATTGCGAGGA CAGAAATATC 
AGGACTTTGG GTTGTGAGGG TATTGCCTTG AACGATACAC CCCTGCAGGA TGTGAACAGC 
CAAGAGAAGG ACAGTAGCCC TCCTGATTTG GATTGCAGTG AGCAGGCTCT GTTGGGCAGC 
ATTCAGAGCC AGGAAAGCCC TAATCCAACC AGCTGCAGTT TGCCAGATGT GTCCAGCAGC 
ACCGAGAGAG TCACAGAGAG CACCGTCATT ACCTTACCAG ATACTGGAAT GCCCACCGAC 
TGCACCGTGG TACAGATGAG TGAAGGCAGG GATGGAGAAG TCAACTTTGG GTCTTCGCAG 
CAAGAAAAAG AAGAGGAGGA AGCTGAGGAT GGAGAAGAAA GAACTATAAA AAGAAAAGAG 
AAATTGTTGG AGTCCCCCAA AGCCATTGTA CCGTACAGTT CAATGCTCAT CTTCAGCTCC 
ACCAATCCCA TAAGAAAAGT ATGTCATTAC ATTGTTAACC TACGATACTT TGAGATGAGC 
ATCCTGCTGG TAATAGCAGC AAGTAGTATA GCCTTAGCAG CGGAGGACCC TGTACTGACC 
AACTCGGACC GCAACAAAGT TCTTCGCTAT TTTGACTATG TTTTCACAGG GGTCTTCACT 
TTTGAGATGA TTATAAAGAT GATTGACCAA GGTTTGATTT TACACGAGGG CTCCTACTTT 
CGTGACCTAT GGAACATCTT GGATTTTGTT GTTGTTGTGG GAGCCTTAAT TGCGTTTGCC 
TTAGCGAATG TTTTGGGGAC TAACAAAGGC CGGGACATCA AAACCATTAA GTCTCTACGT 
GTGTTACGGG TGCTGCGTCC ACTAAAAACA ATCAAACGCC TGCCAAAACT GAAGGCAGTC 
TTTGATTGTG TGGTTAACTC TTTAAAGAAT GTCTTTAATA TTCTCATCGT ATACAAACTC 
TTCATGTTCA TATTTGCTGT AATCGCTGTC CAGCTGTTTA AGGGCAAGTT CTTCTATTGT 
ACAGACAGCT CAAAGGAGAC AGAGAAAGAG TGCATAGGGA GTTACATCGA TCACGAAAAG 
AACAAGATGG AGGTGAAGCG GAGAGAGTGG AAAAGACATG ATTTCCATTA TGACAACATA 
ATATGGGCTC TTCTTACCCT CTTTACTGTA TCAACAGGGG AAGGCTGGCC TCAGGTTCTG 
CAGCACTCTG TTGATATAAC TGAAGAAGAT AGGGGTCCAA GCCGAAGCAA CCGTATGGAG 
ATGTCCATTT TCTATGTTAT CTACTTTGTG GTCTTCCCCT TCTTCTTTGT AAATATATTT 
GTGGCTCTGA TTATCATCAC ATTCCAGGAG CAAGGAGACA AGATGATGGA GGAATGTTCC 
CTAGAAAAGA ATGAGAGAGC CTGCATAGAC TTTGCAATAA GTGCTAAGCC ACTTACACGA 
TATATGCCAC AGAACAGGCA CACATTCCAG TACCGGGTCT GGCACTTCGT GGTCTCCCCA 
TCATTTGAAT ACACTATCAT GGCTATGATT GCACTAAACA CAGTGGTACT CATGATGAAG 
TATTATTCTG CGCCATACAC ATATGAGCTT GCATTAAAGT ATCTCAATAT TGCATTCACA 
ATGCTATTTT CTCTGGAGTG CATCCTGAAA ATAATTGCAT TTGGGTTTTT GAATTACTTT 
CGGGATACTT GGAACATTTT TGACTTCATT ACTGTAATTG GTAGCATCAC GGAAATTATT 
CTAACAGATA GTAAGTTGGT GAACACCAGC AGCTTTAATA TGAGCTTTCT GAAGCTGTTC 
CGAGCGGCGC GACTTATTAA ACTTCTCCGG CAAGGGTACA CCATCCGGAT CCTGCTGTGG 
ACTTTTGTTC AGTCCTTTAA GGCACTTCCT TATGTGTGTC TTCTCATTGC AATGTTGTTC 
TTCATCTATG CCATTATTGG GATGCAGGTG TTTGGCAATA TCCGCCTGGA AGAGGACAGT 
CATATTAATA GGCACAACAA TTTTAGAAGC TTCTTTAGTT CTCTTATGTT ACTCTTCAGG 
AGTGCCACAG GAGAAGCGTG GCAGGAGATC ATGCTTTCAT GCCTTAGTGA TAAGGGTTGT 
GAGCCAGACA CCAGTGCAGC CTCTGGACAC AACGAGAATG AGCGCTGTGG AACTGAACTT 
GCATATGTTT ATTTTGTTTC ATTCATTTTC TTCTGCTCCT TCCTCATGCT AAATCTGTTT 
GTGGCTGTCA TTATGGACAA CTTTGAGTAC TTGACACGGG ACTCTTCCAT TTTGGGACCT 
CATCACTTGG ATGAGTTTGT TCGGATATGG GCTGAATATG ATCGAGCAGC ATGTGGCAGG 
ATCCCCTATA AGGATATGTA CAAATTAGTC CGAGTCATCT CGCCTCCGCT CGGCCTAGGA 
GAGAACTGCC CATACAGGGT GGCATGCAAG AGATTGGTCC TGATGAATAT GCCAGTGTCG 
GAGGACATGA CGGTTCATTT CACATCAACT CTTATGGCCC TAATACGCAC GGCACTGGAT 
ATCAAAATAG CTAAAGGAGG TGCTGACAGG CAACAGCTGG ACTCAGAGCT GCAGAAAGAT 
ATACTTGCCA TTTGGCCAAA TCTCTCCCAG AAAATGATCG ACTTACTTGT GCCAATGCCA 
AGAGCCTCTG ATCTGACGGT GGGGAAGATT TACGCTGCAA TGATGATCAT GGATTACTAT 
AAACAGAGCA AAGCGAAAAA ACAAAGGCAG CAGCTGGAGG AGCAGAAAAA TGCACCTATG 
TTTCAGAGAA TGGAGGCTTC TGCCCTCCCT GAGGAGATCT TATCCAATGC CAAAGCTTTA 
CCATACCTTC AGCAAGACAC TCTATCAGGC CTGGGTGGAT TTCCTTCACT GAGTCCCTTG 
TCCCCAGAAG AAGTATTCCA GCCGCCATGC CTAGACGGGG AAGAGGAGGA AGACTTTGAA 
GAGCAGCTGT CTCTGGTCCT GTCAGACGTA AGCTCCATGC GTCGCTCCTT CTCCACAACT 
GCAGAGAAGC GAGTGCGCAG CTCGTGGCTA GATGAGTTCA CTATGGACAG GAGTAGCACT 
GACTGTGCAT ACAGATACCA GAGGCGCAGT TATCATGCAT CCTTAAGACT GTCTGCACAT 
CGCCTGAATA CAGATACAGG TCACTGGTCA GATAATCACC GTTCTGGCGG AAGGGAGCGA 
GGAAGGTCCA AAGAGAGAAA ACACTTGCTG TCACCCGATC CTTCCCGCTG TAATTCTGAG 
GAAAGGAGCC CACAAGCTGC CTGTGAACAC CGAGATCACC AACTGTCCCC TGGGCATACT 
CCCAGTAGTG TACAGATTCT TGACTCTCTG AGTGAAAGTT GTACCCCTTC AGACACCAGC 
ACCCCAAGAA GAGGCCGTAG ACAGCTTCCT TCTGTGCCTC CAACTCCTAG ACCCCTTATC 
TCTTTCTCCT CGTTAATCCG CCGGCAGAAA CACAGCCCCC CTCCTCAAAA TAAAAATGAT 
ACTATCCTTC CTAGTGAGCC TGGTCTGACT GAGTCTTCCT GCTCCCTTGG GGGTCATCAG 
AGCTCCCAGT CCACACCACA GCATTATATC TCAGAGCCCT ACCTGGCACT GCATGAGGAG 
ATGCAAAGCT CAGACTGCCC AGCAGAGGAC ACACTCACCT TTGAGGTTGC TGTGGCTACT 
AGTCTGGGCC GCTCGAACAC CATTGGCTCT GCCCCTCTAC GTCATGGCTG GCAAATGCCC 
AATGGTCACT ACCGGCGTCG AAGGAAGGGA GGAATTCCAC CAGTGATACC CTCCAGAGAG 
TTAAGTGACA CAGAAGAGGA TGATCGATGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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1-732-210-0262

cacna1e cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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