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EBF4 cDNA ORF clone, Bos taurus(cattle)

Summary

Gene Symbol EBF4
Entrez Gene ID 506470
Full Name early B cell factor 4
General protein information
Preferred Names

early B cell factor 4

Names

transcription factor COE4
Gene Type protein-coding
Organism Bos taurus(cattle)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_015466076.1 XP_015321562.1 transcription factor COE4 isoform X2
XM_015474217.1 XP_015329703.1 transcription factor COE4 isoform X3
XM_010822529.1 XP_010820831.1 transcription factor COE4 isoform X1
XM_025001082.1 XP_024856850.1 transcription factor COE4 isoform X2
XM_025001085.1 XP_024856853.1 transcription factor COE4 isoform X6
XM_015474216.1 XP_015329702.1 transcription factor COE4 isoform X2
XM_025001081.1 XP_024856849.1 transcription factor COE4 isoform X1
XM_025001086.1 XP_024856854.1 transcription factor COE4 isoform X7
XM_025001083.1 XP_024856851.1 transcription factor COE4 isoform X4
XM_015466077.1 XP_015321563.1 transcription factor COE4 isoform X4
XM_025001084.1 XP_024856852.1 transcription factor COE4 isoform X5
XM_003586810.4 XP_003586858.2 transcription factor COE4 isoform X3

Pathways from BioSystems


Homologies


Canis lupus familiaris (dog) EBF4 XP_005634927.1
Mus musculus (house mouse) Ebf4 NP_001103983.1
Rattus norvegicus (Norway rat) Ebf4 NP_001178005.1
Bos taurus (cattle) EBF4 XP_003586858.2
Homo sapiens (human) EBF4 NP_001103984.1

Gene Ontology

The following EBF4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EBF4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa221945 XM_025001081.1
Latest version!		 Bos taurus early B cell factor 4 (EBF4), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $307.30
$439.00
OBa221946 XM_025001083.1
Latest version!		 Bos taurus early B cell factor 4 (EBF4), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $307.30
$439.00
OBa221947 XM_025001084.1
Latest version!		 Bos taurus early B cell factor 4 (EBF4), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $307.30
$439.00
OBa25590 XM_010822529.1
Latest version!		 Bos taurus early B-cell factor 4 (EBF4), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $342.30
$489.00
OBa25590 XM_003586810.4
Latest version!		 Bos taurus early B cell factor 4 (EBF4), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $342.30
$489.00
OBa46570 XM_025001086.1
Latest version!		 Bos taurus early B cell factor 4 (EBF4), transcript variant X7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $342.30
$489.00
OBa92362 XM_025001085.1
Latest version!		 Bos taurus early B cell factor 4 (EBF4), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $342.30
$489.00
OBa92362 XM_015474216.1
Latest version!		 Bos taurus early B-cell factor 4 (EBF4), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $342.30
$489.00
OBa92362 XM_015466076.1
Latest version!		 Bos taurus early B-cell factor 4 (EBF4), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $342.30
$489.00
OBa92363 XM_015474217.1
Latest version!		 Bos taurus early B-cell factor 4 (EBF4), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $342.30
$489.00
OBa94802 XM_015466077.1
Latest version!		 Bos taurus early B-cell factor 4 (EBF4), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $342.30
$489.00
OBa94802 XM_025001082.1
Latest version!		 Bos taurus early B cell factor 4 (EBF4), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $342.30
$489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OBa221945
Clone ID Related Accession (Same CDS sequence) XM_025001081.1
Accession Version XM_025001081.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1815bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-06
Organism Bos taurus(cattle)
Product transcription factor COE4 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGGACGCTG TGCCCCGCGG CGGGCTGAAC CTGAAGGAGG AGCCGCTGCT GCCCGCGGGC 
CTGGGCTCGG TGCGCTCCTG GATGCAGGGC GCGGGCATCC TGGACGCCAG TACCGCGGCG 
CAGAGCAGCT TTCCTCCCCA CAGTGGTGTG GGCCTGGCAC GAGCACACTT TGAGAAGCAG 
CCCCCTTCCA ACCTCAGGAA ATCCAACTTC TTCCACTTCG TGCTGGCCAT GTATGACCGG 
CAGGGGCAGC CGGTGGAGGT GGAGCGCACA GCCTTCATCG ACTTTGTGGA GAAGGACCGA 
GAGCCTGGGG CTGAAAAGAC AAACAACGGA ATCCATTACC GCCTCCGGCT GGTATATAAC 
AACGGTCTTC GGACAGAACA GGATCTCTAC GTGCGTCTCA TTGACTCCAT GTCCAAGCAG 
GCCATCATCT ATGAAGGGCA GGACAAGAAC CCCGAAATGT GCCGTGTGCT GCTCACTCAT 
GAGATCATGT GCAGCCGATG CTGTGACCGG AAGAGCTGTG GCAACCGGAA TGAGACGCCC 
TCAGACCCCG TCATCATTGA CAGGTTCTTC CTCAAGTTCT TCCTCAAATG CAACCAGAAT 
TGCCTAAAGA ACGCGGGGAA TCCCCGAGAC ATGCGCCGCT TCCAGGTGGT GGTGTCCACA 
ACAGTGAGCG TGGACGGACA CGTGCTGGCT GTGTCAGACA ACATGTTTGT GCACAACAAC 
TCCAAGCATG GCCGCAGGGC ACGCCGCCTG GACCCCTCCG AAGCTGCCAC CCCCTGCATC 
AAGGCCATCA GTCCTGGGGA GGGCTGGACC ACAGGCGGCG CCACCGTCAT TGTCATCGGC 
GACAACTTCT TCGACGGGTT ACAGGTCGTG TTTGGGAACG TGCTCGTGTG GAGTGAGCTC 
ATCACGCCCC ACGCCATACG GGTTCAGACG CCCCCACGGC ACATCCCTGG GGTGGTGGAG 
GTGACTCTGT CCTACAAATC CAAGCAGTTT TGCAAGGGAG CCCCGGGACG CTTTGTCTAC 
ACAGCCCTGA ATGAGCCCAC CATTGACTAC GGATTCCAGA GGCTGCAGAA AGTTATTCCC 
AGACACCCCG GAGACCCCGA GAGGCTGCCC AAGGAAGTGT TGCTGAAGCG GGCGGCCGAC 
TTGGCAGAGG CCCTGTACGG AGTGCCCAGC GGCAACCAGG AGCTCCTCCT GAAGCGCGCG 
GCGGACGTGG CCGAGGCTCT GTACAGCGCC CCCCGCGCGC CGGCGCCGCT AGGACCCCTG 
GCGCCCAGCC ACCCGCACCC TGCCGTCGTG GGCATCAACG CCTTCAGCAG CCCGCTGGCC 
ATCGCCGTCG GGGACGCCAC GCCGGGGCCG GAGCCAGGGT ATGCGCGCAG CTGTGGCAGC 
GCGTCCCCGC GCGGGTTCGC GCCCAGCCCC GGCTCACAGC AGAGCGGCTA CGGCGGCAGC 
CTCGGAGCTG GCCTCGGCGG CTACGGGGCC CCGGGCGTGA CCGGCCTCGG TGTGCCCGGG 
TCCCCCAGCT TCCTCAATGG CTCCACGGCC ACCTCACCCT TCGCCATCAT GCCCTCTAGC 
CCCCCGCTGG CCTCTGCCTC CTCCATGTCC CTCCCGGCGG CTGCCCCTAC CACCAGCGTC 
TTCTCCTTCT CGCCTGTCCA CATGATCTCC GCTGTCAAAC AGAGGAGCGC TTTTGCCCCC 
GTGCTGCGCC CGCCCAGCTC CCCACCCCAG GCCTGCCCCA GAACCCACGG AGAGGGGCTT 
CCAGACCAGC CTTTTGAAGA TTCTGACAAG TTTCACTCTC CAGCTCGGGG ACTTCAAGGC 
CTGGCATACT CCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_024856849.1
CDS240..2054
Translation

Target ORF information:

RefSeq Version XM_025001081.1
Organism Bos taurus(cattle)
Definition Bos taurus early B cell factor 4 (EBF4), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_025001081.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGGACGCTG TGCCCCGCGG CGGGCTGAAC CTGAAGGAGG AGCCGCTGCT GCCCGCGGGC 
CTGGGCTCGG TGCGCTCCTG GATGCAGGGC GCGGGCATCC TGGACGCCAG TACCGCGGCG 
CAGAGCAGCT TTCCTCCCCA CAGTGGTGTG GGCCTGGCAC GAGCACACTT TGAGAAGCAG 
CCCCCTTCCA ACCTCAGGAA ATCCAACTTC TTCCACTTCG TGCTGGCCAT GTATGACCGG 
CAGGGGCAGC CGGTGGAGGT GGAGCGCACA GCCTTCATCG ACTTTGTGGA GAAGGACCGA 
GAGCCTGGGG CTGAAAAGAC AAACAACGGA ATCCATTACC GCCTCCGGCT GGTATATAAC 
AACGGTCTTC GGACAGAACA GGATCTCTAC GTGCGTCTCA TTGACTCCAT GTCCAAGCAG 
GCCATCATCT ATGAAGGGCA GGACAAGAAC CCCGAAATGT GCCGTGTGCT GCTCACTCAT 
GAGATCATGT GCAGCCGATG CTGTGACCGG AAGAGCTGTG GCAACCGGAA TGAGACGCCC 
TCAGACCCCG TCATCATTGA CAGGTTCTTC CTCAAGTTCT TCCTCAAATG CAACCAGAAT 
TGCCTAAAGA ACGCGGGGAA TCCCCGAGAC ATGCGCCGCT TCCAGGTGGT GGTGTCCACA 
ACAGTGAGCG TGGACGGACA CGTGCTGGCT GTGTCAGACA ACATGTTTGT GCACAACAAC 
TCCAAGCATG GCCGCAGGGC ACGCCGCCTG GACCCCTCCG AAGCTGCCAC CCCCTGCATC 
AAGGCCATCA GTCCTGGGGA GGGCTGGACC ACAGGCGGCG CCACCGTCAT TGTCATCGGC 
GACAACTTCT TCGACGGGTT ACAGGTCGTG TTTGGGAACG TGCTCGTGTG GAGTGAGCTC 
ATCACGCCCC ACGCCATACG GGTTCAGACG CCCCCACGGC ACATCCCTGG GGTGGTGGAG 
GTGACTCTGT CCTACAAATC CAAGCAGTTT TGCAAGGGAG CCCCGGGACG CTTTGTCTAC 
ACAGCCCTGA ATGAGCCCAC CATTGACTAC GGATTCCAGA GGCTGCAGAA AGTTATTCCC 
AGACACCCCG GAGACCCCGA GAGGCTGCCC AAGGAAGTGT TGCTGAAGCG GGCGGCCGAC 
TTGGCAGAGG CCCTGTACGG AGTGCCCAGC GGCAACCAGG AGCTCCTCCT GAAGCGCGCG 
GCGGACGTGG CCGAGGCTCT GTACAGCGCC CCCCGCGCGC CGGCGCCGCT AGGACCCCTG 
GCGCCCAGCC ACCCGCACCC TGCCGTCGTG GGCATCAACG CCTTCAGCAG CCCGCTGGCC 
ATCGCCGTCG GGGACGCCAC GCCGGGGCCG GAGCCAGGGT ATGCGCGCAG CTGTGGCAGC 
GCGTCCCCGC GCGGGTTCGC GCCCAGCCCC GGCTCACAGC AGAGCGGCTA CGGCGGCAGC 
CTCGGAGCTG GCCTCGGCGG CTACGGGGCC CCGGGCGTGA CCGGCCTCGG TGTGCCCGGG 
TCCCCCAGCT TCCTCAATGG CTCCACGGCC ACCTCACCCT TCGCCATCAT GCCCTCTAGC 
CCCCCGCTGG CCTCTGCCTC CTCCATGTCC CTCCCGGCGG CTGCCCCTAC CACCAGCGTC 
TTCTCCTTCT CGCCTGTCCA CATGATCTCC GCTGTCAAAC AGAGGAGCGC TTTTGCCCCC 
GTGCTGCGCC CGCCCAGCTC CCCACCCCAG GCCTGCCCCA GAACCCACGG AGAGGGGCTT 
CCAGACCAGC CTTTTGAAGA TTCTGACAAG TTTCACTCTC CAGCTCGGGG ACTTCAAGGC 
CTGGCATACT CCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa221946
Clone ID Related Accession (Same CDS sequence) XM_025001083.1
Accession Version XM_025001083.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1797bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-06
Organism Bos taurus(cattle)
Product transcription factor COE4 isoform X4
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGGACGCTG TGCCCCGCGG CGGGCTGAAC CTGAAGGAGG AGCCGCTGCT GCCCGCGGGC 
CTGGGCTCGG TGCGCTCCTG GATGCAGGGC GCGGGCATCC TGGACGCCAG TACCGCGGCG 
CAGAGTGGTG TGGGCCTGGC ACGAGCACAC TTTGAGAAGC AGCCCCCTTC CAACCTCAGG 
AAATCCAACT TCTTCCACTT CGTGCTGGCC ATGTATGACC GGCAGGGGCA GCCGGTGGAG 
GTGGAGCGCA CAGCCTTCAT CGACTTTGTG GAGAAGGACC GAGAGCCTGG GGCTGAAAAG 
ACAAACAACG GAATCCATTA CCGCCTCCGG CTGGTATATA ACAACGGTCT TCGGACAGAA 
CAGGATCTCT ACGTGCGTCT CATTGACTCC ATGTCCAAGC AGGCCATCAT CTATGAAGGG 
CAGGACAAGA ACCCCGAAAT GTGCCGTGTG CTGCTCACTC ATGAGATCAT GTGCAGCCGA 
TGCTGTGACC GGAAGAGCTG TGGCAACCGG AATGAGACGC CCTCAGACCC CGTCATCATT 
GACAGGTTCT TCCTCAAGTT CTTCCTCAAA TGCAACCAGA ATTGCCTAAA GAACGCGGGG 
AATCCCCGAG ACATGCGCCG CTTCCAGGTG GTGGTGTCCA CAACAGTGAG CGTGGACGGA 
CACGTGCTGG CTGTGTCAGA CAACATGTTT GTGCACAACA ACTCCAAGCA TGGCCGCAGG 
GCACGCCGCC TGGACCCCTC CGAAGCTGCC ACCCCCTGCA TCAAGGCCAT CAGTCCTGGG 
GAGGGCTGGA CCACAGGCGG CGCCACCGTC ATTGTCATCG GCGACAACTT CTTCGACGGG 
TTACAGGTCG TGTTTGGGAA CGTGCTCGTG TGGAGTGAGC TCATCACGCC CCACGCCATA 
CGGGTTCAGA CGCCCCCACG GCACATCCCT GGGGTGGTGG AGGTGACTCT GTCCTACAAA 
TCCAAGCAGT TTTGCAAGGG AGCCCCGGGA CGCTTTGTCT ACACAGCCCT GAATGAGCCC 
ACCATTGACT ACGGATTCCA GAGGCTGCAG AAAGTTATTC CCAGACACCC CGGAGACCCC 
GAGAGGCTGC CCAAGGAAGT GTTGCTGAAG CGGGCGGCCG ACTTGGCAGA GGCCCTGTAC 
GGAGTGCCCA GCGGCAACCA GGAGCTCCTC CTGAAGCGCG CGGCGGACGT GGCCGAGGCT 
CTGTACAGCG CCCCCCGCGC GCCGGCGCCG CTAGGACCCC TGGCGCCCAG CCACCCGCAC 
CCTGCCGTCG TGGGCATCAA CGCCTTCAGC AGCCCGCTGG CCATCGCCGT CGGGGACGCC 
ACGCCGGGGC CGGAGCCAGG GTATGCGCGC AGCTGTGGCA GCGCGTCCCC GCGCGGGTTC 
GCGCCCAGCC CCGGCTCACA GCAGAGCGGC TACGGCGGCA GCCTCGGAGC TGGCCTCGGC 
GGCTACGGGG CCCCGGGCGT GACCGGCCTC GGTGTGCCCG GGTCCCCCAG CTTCCTCAAT 
GGCTCCACGG CCACCTCACC CTTCGCCATC ATGCCCTCTA GCCCCCCGCT GGCCTCTGCC 
TCCTCCATGT CCCTCCCGGC GGCTGCCCCT ACCACCAGCG TCTTCTCCTT CTCGCCTGTC 
CACATGATCT CCGCTGTCAA ACAGAGGAGC GCTTTTGCCC CCGTGCTGCG CCCGCCCAGC 
TCCCCACCCC AGGCCTGCCC CAGAACCCAC GGAGAGGGGC TTCCAGACCA GCCTTTTGAA 
GATTCTGACA AGTTTCACTC TCCAGCTCGG GGACTTCAAG GCCTGGCATA CTCCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_024856851.1
CDS240..2036
Translation

Target ORF information:

RefSeq Version XM_025001083.1
Organism Bos taurus(cattle)
Definition Bos taurus early B cell factor 4 (EBF4), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_025001083.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGGACGCTG TGCCCCGCGG CGGGCTGAAC CTGAAGGAGG AGCCGCTGCT GCCCGCGGGC 
CTGGGCTCGG TGCGCTCCTG GATGCAGGGC GCGGGCATCC TGGACGCCAG TACCGCGGCG 
CAGAGTGGTG TGGGCCTGGC ACGAGCACAC TTTGAGAAGC AGCCCCCTTC CAACCTCAGG 
AAATCCAACT TCTTCCACTT CGTGCTGGCC ATGTATGACC GGCAGGGGCA GCCGGTGGAG 
GTGGAGCGCA CAGCCTTCAT CGACTTTGTG GAGAAGGACC GAGAGCCTGG GGCTGAAAAG 
ACAAACAACG GAATCCATTA CCGCCTCCGG CTGGTATATA ACAACGGTCT TCGGACAGAA 
CAGGATCTCT ACGTGCGTCT CATTGACTCC ATGTCCAAGC AGGCCATCAT CTATGAAGGG 
CAGGACAAGA ACCCCGAAAT GTGCCGTGTG CTGCTCACTC ATGAGATCAT GTGCAGCCGA 
TGCTGTGACC GGAAGAGCTG TGGCAACCGG AATGAGACGC CCTCAGACCC CGTCATCATT 
GACAGGTTCT TCCTCAAGTT CTTCCTCAAA TGCAACCAGA ATTGCCTAAA GAACGCGGGG 
AATCCCCGAG ACATGCGCCG CTTCCAGGTG GTGGTGTCCA CAACAGTGAG CGTGGACGGA 
CACGTGCTGG CTGTGTCAGA CAACATGTTT GTGCACAACA ACTCCAAGCA TGGCCGCAGG 
GCACGCCGCC TGGACCCCTC CGAAGCTGCC ACCCCCTGCA TCAAGGCCAT CAGTCCTGGG 
GAGGGCTGGA CCACAGGCGG CGCCACCGTC ATTGTCATCG GCGACAACTT CTTCGACGGG 
TTACAGGTCG TGTTTGGGAA CGTGCTCGTG TGGAGTGAGC TCATCACGCC CCACGCCATA 
CGGGTTCAGA CGCCCCCACG GCACATCCCT GGGGTGGTGG AGGTGACTCT GTCCTACAAA 
TCCAAGCAGT TTTGCAAGGG AGCCCCGGGA CGCTTTGTCT ACACAGCCCT GAATGAGCCC 
ACCATTGACT ACGGATTCCA GAGGCTGCAG AAAGTTATTC CCAGACACCC CGGAGACCCC 
GAGAGGCTGC CCAAGGAAGT GTTGCTGAAG CGGGCGGCCG ACTTGGCAGA GGCCCTGTAC 
GGAGTGCCCA GCGGCAACCA GGAGCTCCTC CTGAAGCGCG CGGCGGACGT GGCCGAGGCT 
CTGTACAGCG CCCCCCGCGC GCCGGCGCCG CTAGGACCCC TGGCGCCCAG CCACCCGCAC 
CCTGCCGTCG TGGGCATCAA CGCCTTCAGC AGCCCGCTGG CCATCGCCGT CGGGGACGCC 
ACGCCGGGGC CGGAGCCAGG GTATGCGCGC AGCTGTGGCA GCGCGTCCCC GCGCGGGTTC 
GCGCCCAGCC CCGGCTCACA GCAGAGCGGC TACGGCGGCA GCCTCGGAGC TGGCCTCGGC 
GGCTACGGGG CCCCGGGCGT GACCGGCCTC GGTGTGCCCG GGTCCCCCAG CTTCCTCAAT 
GGCTCCACGG CCACCTCACC CTTCGCCATC ATGCCCTCTA GCCCCCCGCT GGCCTCTGCC 
TCCTCCATGT CCCTCCCGGC GGCTGCCCCT ACCACCAGCG TCTTCTCCTT CTCGCCTGTC 
CACATGATCT CCGCTGTCAA ACAGAGGAGC GCTTTTGCCC CCGTGCTGCG CCCGCCCAGC 
TCCCCACCCC AGGCCTGCCC CAGAACCCAC GGAGAGGGGC TTCCAGACCA GCCTTTTGAA 
GATTCTGACA AGTTTCACTC TCCAGCTCGG GGACTTCAAG GCCTGGCATA CTCCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa221947
Clone ID Related Accession (Same CDS sequence) XM_025001084.1
Accession Version XM_025001084.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1734bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-06
Organism Bos taurus(cattle)
Product transcription factor COE4 isoform X5
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGCAGGGCG CGGGCATCCT GGACGCCAGT ACCGCGGCGC AGAGCAGCTT TCCTCCCCAC 
AGTGGTGTGG GCCTGGCACG AGCACACTTT GAGAAGCAGC CCCCTTCCAA CCTCAGGAAA 
TCCAACTTCT TCCACTTCGT GCTGGCCATG TATGACCGGC AGGGGCAGCC GGTGGAGGTG 
GAGCGCACAG CCTTCATCGA CTTTGTGGAG AAGGACCGAG AGCCTGGGGC TGAAAAGACA 
AACAACGGAA TCCATTACCG CCTCCGGCTG GTATATAACA ACGGTCTTCG GACAGAACAG 
GATCTCTACG TGCGTCTCAT TGACTCCATG TCCAAGCAGG CCATCATCTA TGAAGGGCAG 
GACAAGAACC CCGAAATGTG CCGTGTGCTG CTCACTCATG AGATCATGTG CAGCCGATGC 
TGTGACCGGA AGAGCTGTGG CAACCGGAAT GAGACGCCCT CAGACCCCGT CATCATTGAC 
AGGTTCTTCC TCAAGTTCTT CCTCAAATGC AACCAGAATT GCCTAAAGAA CGCGGGGAAT 
CCCCGAGACA TGCGCCGCTT CCAGGTGGTG GTGTCCACAA CAGTGAGCGT GGACGGACAC 
GTGCTGGCTG TGTCAGACAA CATGTTTGTG CACAACAACT CCAAGCATGG CCGCAGGGCA 
CGCCGCCTGG ACCCCTCCGA AGCTGCCACC CCCTGCATCA AGGCCATCAG TCCTGGGGAG 
GGCTGGACCA CAGGCGGCGC CACCGTCATT GTCATCGGCG ACAACTTCTT CGACGGGTTA 
CAGGTCGTGT TTGGGAACGT GCTCGTGTGG AGTGAGCTCA TCACGCCCCA CGCCATACGG 
GTTCAGACGC CCCCACGGCA CATCCCTGGG GTGGTGGAGG TGACTCTGTC CTACAAATCC 
AAGCAGTTTT GCAAGGGAGC CCCGGGACGC TTTGTCTACA CAGCCCTGAA TGAGCCCACC 
ATTGACTACG GATTCCAGAG GCTGCAGAAA GTTATTCCCA GACACCCCGG AGACCCCGAG 
AGGCTGCCCA AGGAAGTGTT GCTGAAGCGG GCGGCCGACT TGGCAGAGGC CCTGTACGGA 
GTGCCCAGCG GCAACCAGGA GCTCCTCCTG AAGCGCGCGG CGGACGTGGC CGAGGCTCTG 
TACAGCGCCC CCCGCGCGCC GGCGCCGCTA GGACCCCTGG CGCCCAGCCA CCCGCACCCT 
GCCGTCGTGG GCATCAACGC CTTCAGCAGC CCGCTGGCCA TCGCCGTCGG GGACGCCACG 
CCGGGGCCGG AGCCAGGGTA TGCGCGCAGC TGTGGCAGCG CGTCCCCGCG CGGGTTCGCG 
CCCAGCCCCG GCTCACAGCA GAGCGGCTAC GGCGGCAGCC TCGGAGCTGG CCTCGGCGGC 
TACGGGGCCC CGGGCGTGAC CGGCCTCGGT GTGCCCGGGT CCCCCAGCTT CCTCAATGGC 
TCCACGGCCA CCTCACCCTT CGCCATCATG CCCTCTAGCC CCCCGCTGGC CTCTGCCTCC 
TCCATGTCCC TCCCGGCGGC TGCCCCTACC ACCAGCGTCT TCTCCTTCTC GCCTGTCCAC 
ATGATCTCCG CTGTCAAACA GAGGAGCGCT TTTGCCCCCG TGCTGCGCCC GCCCAGCTCC 
CCACCCCAGG CCTGCCCCAG AACCCACGGA GAGGGGCTTC CAGACCAGCC TTTTGAAGAT 
TCTGACAAGT TTCACTCTCC AGCTCGGGGA CTTCAAGGCC TGGCATACTC CTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_024856852.1
CDS317..2050
Translation

Target ORF information:

RefSeq Version XM_025001084.1
Organism Bos taurus(cattle)
Definition Bos taurus early B cell factor 4 (EBF4), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_025001084.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGCAGGGCG CGGGCATCCT GGACGCCAGT ACCGCGGCGC AGAGCAGCTT TCCTCCCCAC 
AGTGGTGTGG GCCTGGCACG AGCACACTTT GAGAAGCAGC CCCCTTCCAA CCTCAGGAAA 
TCCAACTTCT TCCACTTCGT GCTGGCCATG TATGACCGGC AGGGGCAGCC GGTGGAGGTG 
GAGCGCACAG CCTTCATCGA CTTTGTGGAG AAGGACCGAG AGCCTGGGGC TGAAAAGACA 
AACAACGGAA TCCATTACCG CCTCCGGCTG GTATATAACA ACGGTCTTCG GACAGAACAG 
GATCTCTACG TGCGTCTCAT TGACTCCATG TCCAAGCAGG CCATCATCTA TGAAGGGCAG 
GACAAGAACC CCGAAATGTG CCGTGTGCTG CTCACTCATG AGATCATGTG CAGCCGATGC 
TGTGACCGGA AGAGCTGTGG CAACCGGAAT GAGACGCCCT CAGACCCCGT CATCATTGAC 
AGGTTCTTCC TCAAGTTCTT CCTCAAATGC AACCAGAATT GCCTAAAGAA CGCGGGGAAT 
CCCCGAGACA TGCGCCGCTT CCAGGTGGTG GTGTCCACAA CAGTGAGCGT GGACGGACAC 
GTGCTGGCTG TGTCAGACAA CATGTTTGTG CACAACAACT CCAAGCATGG CCGCAGGGCA 
CGCCGCCTGG ACCCCTCCGA AGCTGCCACC CCCTGCATCA AGGCCATCAG TCCTGGGGAG 
GGCTGGACCA CAGGCGGCGC CACCGTCATT GTCATCGGCG ACAACTTCTT CGACGGGTTA 
CAGGTCGTGT TTGGGAACGT GCTCGTGTGG AGTGAGCTCA TCACGCCCCA CGCCATACGG 
GTTCAGACGC CCCCACGGCA CATCCCTGGG GTGGTGGAGG TGACTCTGTC CTACAAATCC 
AAGCAGTTTT GCAAGGGAGC CCCGGGACGC TTTGTCTACA CAGCCCTGAA TGAGCCCACC 
ATTGACTACG GATTCCAGAG GCTGCAGAAA GTTATTCCCA GACACCCCGG AGACCCCGAG 
AGGCTGCCCA AGGAAGTGTT GCTGAAGCGG GCGGCCGACT TGGCAGAGGC CCTGTACGGA 
GTGCCCAGCG GCAACCAGGA GCTCCTCCTG AAGCGCGCGG CGGACGTGGC CGAGGCTCTG 
TACAGCGCCC CCCGCGCGCC GGCGCCGCTA GGACCCCTGG CGCCCAGCCA CCCGCACCCT 
GCCGTCGTGG GCATCAACGC CTTCAGCAGC CCGCTGGCCA TCGCCGTCGG GGACGCCACG 
CCGGGGCCGG AGCCAGGGTA TGCGCGCAGC TGTGGCAGCG CGTCCCCGCG CGGGTTCGCG 
CCCAGCCCCG GCTCACAGCA GAGCGGCTAC GGCGGCAGCC TCGGAGCTGG CCTCGGCGGC 
TACGGGGCCC CGGGCGTGAC CGGCCTCGGT GTGCCCGGGT CCCCCAGCTT CCTCAATGGC 
TCCACGGCCA CCTCACCCTT CGCCATCATG CCCTCTAGCC CCCCGCTGGC CTCTGCCTCC 
TCCATGTCCC TCCCGGCGGC TGCCCCTACC ACCAGCGTCT TCTCCTTCTC GCCTGTCCAC 
ATGATCTCCG CTGTCAAACA GAGGAGCGCT TTTGCCCCCG TGCTGCGCCC GCCCAGCTCC 
CCACCCCAGG CCTGCCCCAG AACCCACGGA GAGGGGCTTC CAGACCAGCC TTTTGAAGAT 
TCTGACAAGT TTCACTCTCC AGCTCGGGGA CTTCAAGGCC TGGCATACTC CTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa25590
Clone ID Related Accession (Same CDS sequence) XM_010822529.1 , XM_003586810.4
Accession Version XM_010822529.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1809bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-25
Organism Bos taurus(cattle)
Product transcription factor COE4 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646145.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGTTCCCTG CTCAGGACGC TGTGCCCCGC GGCGGGCTGA ACCTGAAGGA GGAGCCGCTG 
CTGCCCGCGG GCCTGGGCTC GGTGCGCTCC TGGATGCAGG GCGCGGGCAT CCTGGACGCC 
AGTACCGCGG CGCAGAGTGG TGTGGGCCTG GCACGAGCAC ACTTTGAGAA GCAGCCCCCT 
TCCAACCTCA GGAAATCCAA CTTCTTCCAC TTCGTGCTGG CCATGTATGA CCGGCAGGGG 
CAGCCGGTGG AGGTGGAGCG CACAGCCTTC ATCGACTTTG TGGAGAAGGA CCGAGAGCCT 
GGGGCTGAAA AGACAAACAA CGGAATCCAT TACCGCCTCC GGCTGGTATA TAACAACGGT 
CTTCGGACAG AACAGGATCT CTACGTGCGT CTCATTGACT CCATGTCCAA GCAGGCCATC 
ATCTATGAAG GGCAGGACAA GAACCCCGAA ATGTGCCGTG TGCTGCTCAC TCATGAGATC 
ATGTGCAGCC GATGCTGTGA CCGGAAGAGC TGTGGCAACC GGAATGAGAC GCCCTCAGAC 
CCCGTCATCA TTGACAGGTT CTTCCTCAAG TTCTTCCTCA AATGCAACCA GAATTGCCTA 
AAGAACGCGG GGAATCCCCG AGACATGCGC CGCTTCCAGG TGGTGGTGTC CACAACAGTG 
AGCGTGGACG GACACGTGCT GGCTGTGTCA GACAACATGT TTGTGCACAA CAACTCCAAG 
CATGGCCGCA GGGCACGCCG CCTGGACCCC TCCGAAGCTG CCACCCCCTG CATCAAGGCC 
ATCAGTCCTG GGGAGGGCTG GACCACAGGC GGCGCCACCG TCATTGTCAT CGGCGACAAC 
TTCTTCGACG GGTTACAGGT CGTGTTTGGG AACGTGCTCG TGTGGAGTGA GCTCATCACG 
CCCCACGCCA TACGGGTTCA GACGCCCCCA CGGCACATCC CTGGGGTGGT GGAGGTGACT 
CTGTCCTACA AATCCAAGCA GTTTTGCAAG GGAGCCCCGG GACGCTTTGT CTACACAGCC 
CTGAATGAGC CCACCATTGA CTACGGATTC CAGAGGCTGC AGAAAGTTAT TCCCAGACAC 
CCCGGAGACC CCGAGAGGCT GCCCAAGGAA GTGTTGCTGA AGCGGGCGGC CGACTTGGCA 
GAGGCCCTGT ACGGAGTGCC CAGCGGCAAC CAGGAGCTCC TCCTGAAGCG CGCGGCGGAC 
GTGGCCGAGG CTCTGTACAG CGCCCCCCGC GCGCCGGCGC CGCTAGGACC CCTGGCGCCC 
AGCCACCCGC ACCCTGCCGT CGTGGGCATC AACGCCTTCA GCAGCCCGCT GGCCATCGCC 
GTCGGGGACG CCACGCCGGG GCCGGAGCCA GGGTATGCGC GCAGCTGTGG CAGCGCGTCC 
CCGCGCGGGT TCGCGCCCAG CCCCGGCTCA CAGCAGAGCG GCTACGGCGG CAGCCTCGGA 
GCTGGCCTCG GCGGCTACGG GGCCCCGGGC GTGACCGGCC TCGGTGTGCC CGGGTCCCCC 
AGCTTCCTCA ATGGCTCCAC GGCCACCTCA CCCTTCGCCA TCATGCCCTC TAGCCCCCCG 
CTGGCCTCTG CCTCCTCCAT GTCCCTCCCG GCGGCTGCCC CTACCACCAG CGTCTTCTCC 
TTCTCGCCTG TCCACATGAT CTCCGCTGTC AAACAGAGGA GCGCTTTTGC CCCCGTGCTG 
CGCCCGCCCA GCTCCCCACC CCAGGCCTGC CCCAGAACCC ACGGAGAGGG GCTTCCAGAC 
CAGCCTTTTG AAGATTCTGA CAAGTTTCAC TCTCCAGCTC GGGGACTTCA AGGCCTGGCA 
TACTCCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_010820831.1
CDS16..1824
Translation

Target ORF information:

RefSeq Version XM_010822529.1
Organism Bos taurus(cattle)
Definition Bos taurus early B-cell factor 4 (EBF4), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_010822529.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGTTCCCTG CTCAGGACGC TGTGCCCCGC GGCGGGCTGA ACCTGAAGGA GGAGCCGCTG 
CTGCCCGCGG GCCTGGGCTC GGTGCGCTCC TGGATGCAGG GCGCGGGCAT CCTGGACGCC 
AGTACCGCGG CGCAGAGTGG TGTGGGCCTG GCACGAGCAC ACTTTGAGAA GCAGCCCCCT 
TCCAACCTCA GGAAATCCAA CTTCTTCCAC TTCGTGCTGG CCATGTATGA CCGGCAGGGG 
CAGCCGGTGG AGGTGGAGCG CACAGCCTTC ATCGACTTTG TGGAGAAGGA CCGAGAGCCT 
GGGGCTGAAA AGACAAACAA CGGAATCCAT TACCGCCTCC GGCTGGTATA TAACAACGGT 
CTTCGGACAG AACAGGATCT CTACGTGCGT CTCATTGACT CCATGTCCAA GCAGGCCATC 
ATCTATGAAG GGCAGGACAA GAACCCCGAA ATGTGCCGTG TGCTGCTCAC TCATGAGATC 
ATGTGCAGCC GATGCTGTGA CCGGAAGAGC TGTGGCAACC GGAATGAGAC GCCCTCAGAC 
CCCGTCATCA TTGACAGGTT CTTCCTCAAG TTCTTCCTCA AATGCAACCA GAATTGCCTA 
AAGAACGCGG GGAATCCCCG AGACATGCGC CGCTTCCAGG TGGTGGTGTC CACAACAGTG 
AGCGTGGACG GACACGTGCT GGCTGTGTCA GACAACATGT TTGTGCACAA CAACTCCAAG 
CATGGCCGCA GGGCACGCCG CCTGGACCCC TCCGAAGCTG CCACCCCCTG CATCAAGGCC 
ATCAGTCCTG GGGAGGGCTG GACCACAGGC GGCGCCACCG TCATTGTCAT CGGCGACAAC 
TTCTTCGACG GGTTACAGGT CGTGTTTGGG AACGTGCTCG TGTGGAGTGA GCTCATCACG 
CCCCACGCCA TACGGGTTCA GACGCCCCCA CGGCACATCC CTGGGGTGGT GGAGGTGACT 
CTGTCCTACA AATCCAAGCA GTTTTGCAAG GGAGCCCCGG GACGCTTTGT CTACACAGCC 
CTGAATGAGC CCACCATTGA CTACGGATTC CAGAGGCTGC AGAAAGTTAT TCCCAGACAC 
CCCGGAGACC CCGAGAGGCT GCCCAAGGAA GTGTTGCTGA AGCGGGCGGC CGACTTGGCA 
GAGGCCCTGT ACGGAGTGCC CAGCGGCAAC CAGGAGCTCC TCCTGAAGCG CGCGGCGGAC 
GTGGCCGAGG CTCTGTACAG CGCCCCCCGC GCGCCGGCGC CGCTAGGACC CCTGGCGCCC 
AGCCACCCGC ACCCTGCCGT CGTGGGCATC AACGCCTTCA GCAGCCCGCT GGCCATCGCC 
GTCGGGGACG CCACGCCGGG GCCGGAGCCA GGGTATGCGC GCAGCTGTGG CAGCGCGTCC 
CCGCGCGGGT TCGCGCCCAG CCCCGGCTCA CAGCAGAGCG GCTACGGCGG CAGCCTCGGA 
GCTGGCCTCG GCGGCTACGG GGCCCCGGGC GTGACCGGCC TCGGTGTGCC CGGGTCCCCC 
AGCTTCCTCA ATGGCTCCAC GGCCACCTCA CCCTTCGCCA TCATGCCCTC TAGCCCCCCG 
CTGGCCTCTG CCTCCTCCAT GTCCCTCCCG GCGGCTGCCC CTACCACCAG CGTCTTCTCC 
TTCTCGCCTG TCCACATGAT CTCCGCTGTC AAACAGAGGA GCGCTTTTGC CCCCGTGCTG 
CGCCCGCCCA GCTCCCCACC CCAGGCCTGC CCCAGAACCC ACGGAGAGGG GCTTCCAGAC 
CAGCCTTTTG AAGATTCTGA CAAGTTTCAC TCTCCAGCTC GGGGACTTCA AGGCCTGGCA 
TACTCCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa25590
Clone ID Related Accession (Same CDS sequence) XM_010822529.1 , XM_003586810.4
Accession Version XM_003586810.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1809bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-06
Organism Bos taurus(cattle)
Product transcription factor COE4 isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003586810.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGTTCCCTG CTCAGGACGC TGTGCCCCGC GGCGGGCTGA ACCTGAAGGA GGAGCCGCTG 
CTGCCCGCGG GCCTGGGCTC GGTGCGCTCC TGGATGCAGG GCGCGGGCAT CCTGGACGCC 
AGTACCGCGG CGCAGAGTGG TGTGGGCCTG GCACGAGCAC ACTTTGAGAA GCAGCCCCCT 
TCCAACCTCA GGAAATCCAA CTTCTTCCAC TTCGTGCTGG CCATGTATGA CCGGCAGGGG 
CAGCCGGTGG AGGTGGAGCG CACAGCCTTC ATCGACTTTG TGGAGAAGGA CCGAGAGCCT 
GGGGCTGAAA AGACAAACAA CGGAATCCAT TACCGCCTCC GGCTGGTATA TAACAACGGT 
CTTCGGACAG AACAGGATCT CTACGTGCGT CTCATTGACT CCATGTCCAA GCAGGCCATC 
ATCTATGAAG GGCAGGACAA GAACCCCGAA ATGTGCCGTG TGCTGCTCAC TCATGAGATC 
ATGTGCAGCC GATGCTGTGA CCGGAAGAGC TGTGGCAACC GGAATGAGAC GCCCTCAGAC 
CCCGTCATCA TTGACAGGTT CTTCCTCAAG TTCTTCCTCA AATGCAACCA GAATTGCCTA 
AAGAACGCGG GGAATCCCCG AGACATGCGC CGCTTCCAGG TGGTGGTGTC CACAACAGTG 
AGCGTGGACG GACACGTGCT GGCTGTGTCA GACAACATGT TTGTGCACAA CAACTCCAAG 
CATGGCCGCA GGGCACGCCG CCTGGACCCC TCCGAAGCTG CCACCCCCTG CATCAAGGCC 
ATCAGTCCTG GGGAGGGCTG GACCACAGGC GGCGCCACCG TCATTGTCAT CGGCGACAAC 
TTCTTCGACG GGTTACAGGT CGTGTTTGGG AACGTGCTCG TGTGGAGTGA GCTCATCACG 
CCCCACGCCA TACGGGTTCA GACGCCCCCA CGGCACATCC CTGGGGTGGT GGAGGTGACT 
CTGTCCTACA AATCCAAGCA GTTTTGCAAG GGAGCCCCGG GACGCTTTGT CTACACAGCC 
CTGAATGAGC CCACCATTGA CTACGGATTC CAGAGGCTGC AGAAAGTTAT TCCCAGACAC 
CCCGGAGACC CCGAGAGGCT GCCCAAGGAA GTGTTGCTGA AGCGGGCGGC CGACTTGGCA 
GAGGCCCTGT ACGGAGTGCC CAGCGGCAAC CAGGAGCTCC TCCTGAAGCG CGCGGCGGAC 
GTGGCCGAGG CTCTGTACAG CGCCCCCCGC GCGCCGGCGC CGCTAGGACC CCTGGCGCCC 
AGCCACCCGC ACCCTGCCGT CGTGGGCATC AACGCCTTCA GCAGCCCGCT GGCCATCGCC 
GTCGGGGACG CCACGCCGGG GCCGGAGCCA GGGTATGCGC GCAGCTGTGG CAGCGCGTCC 
CCGCGCGGGT TCGCGCCCAG CCCCGGCTCA CAGCAGAGCG GCTACGGCGG CAGCCTCGGA 
GCTGGCCTCG GCGGCTACGG GGCCCCGGGC GTGACCGGCC TCGGTGTGCC CGGGTCCCCC 
AGCTTCCTCA ATGGCTCCAC GGCCACCTCA CCCTTCGCCA TCATGCCCTC TAGCCCCCCG 
CTGGCCTCTG CCTCCTCCAT GTCCCTCCCG GCGGCTGCCC CTACCACCAG CGTCTTCTCC 
TTCTCGCCTG TCCACATGAT CTCCGCTGTC AAACAGAGGA GCGCTTTTGC CCCCGTGCTG 
CGCCCGCCCA GCTCCCCACC CCAGGCCTGC CCCAGAACCC ACGGAGAGGG GCTTCCAGAC 
CAGCCTTTTG AAGATTCTGA CAAGTTTCAC TCTCCAGCTC GGGGACTTCA AGGCCTGGCA 
TACTCCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_003586858.2
CDS16..1824
Translation

Target ORF information:

RefSeq Version XM_003586810.4
Organism Bos taurus(cattle)
Definition Bos taurus early B cell factor 4 (EBF4), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_003586810.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGTTCCCTG CTCAGGACGC TGTGCCCCGC GGCGGGCTGA ACCTGAAGGA GGAGCCGCTG 
CTGCCCGCGG GCCTGGGCTC GGTGCGCTCC TGGATGCAGG GCGCGGGCAT CCTGGACGCC 
AGTACCGCGG CGCAGAGTGG TGTGGGCCTG GCACGAGCAC ACTTTGAGAA GCAGCCCCCT 
TCCAACCTCA GGAAATCCAA CTTCTTCCAC TTCGTGCTGG CCATGTATGA CCGGCAGGGG 
CAGCCGGTGG AGGTGGAGCG CACAGCCTTC ATCGACTTTG TGGAGAAGGA CCGAGAGCCT 
GGGGCTGAAA AGACAAACAA CGGAATCCAT TACCGCCTCC GGCTGGTATA TAACAACGGT 
CTTCGGACAG AACAGGATCT CTACGTGCGT CTCATTGACT CCATGTCCAA GCAGGCCATC 
ATCTATGAAG GGCAGGACAA GAACCCCGAA ATGTGCCGTG TGCTGCTCAC TCATGAGATC 
ATGTGCAGCC GATGCTGTGA CCGGAAGAGC TGTGGCAACC GGAATGAGAC GCCCTCAGAC 
CCCGTCATCA TTGACAGGTT CTTCCTCAAG TTCTTCCTCA AATGCAACCA GAATTGCCTA 
AAGAACGCGG GGAATCCCCG AGACATGCGC CGCTTCCAGG TGGTGGTGTC CACAACAGTG 
AGCGTGGACG GACACGTGCT GGCTGTGTCA GACAACATGT TTGTGCACAA CAACTCCAAG 
CATGGCCGCA GGGCACGCCG CCTGGACCCC TCCGAAGCTG CCACCCCCTG CATCAAGGCC 
ATCAGTCCTG GGGAGGGCTG GACCACAGGC GGCGCCACCG TCATTGTCAT CGGCGACAAC 
TTCTTCGACG GGTTACAGGT CGTGTTTGGG AACGTGCTCG TGTGGAGTGA GCTCATCACG 
CCCCACGCCA TACGGGTTCA GACGCCCCCA CGGCACATCC CTGGGGTGGT GGAGGTGACT 
CTGTCCTACA AATCCAAGCA GTTTTGCAAG GGAGCCCCGG GACGCTTTGT CTACACAGCC 
CTGAATGAGC CCACCATTGA CTACGGATTC CAGAGGCTGC AGAAAGTTAT TCCCAGACAC 
CCCGGAGACC CCGAGAGGCT GCCCAAGGAA GTGTTGCTGA AGCGGGCGGC CGACTTGGCA 
GAGGCCCTGT ACGGAGTGCC CAGCGGCAAC CAGGAGCTCC TCCTGAAGCG CGCGGCGGAC 
GTGGCCGAGG CTCTGTACAG CGCCCCCCGC GCGCCGGCGC CGCTAGGACC CCTGGCGCCC 
AGCCACCCGC ACCCTGCCGT CGTGGGCATC AACGCCTTCA GCAGCCCGCT GGCCATCGCC 
GTCGGGGACG CCACGCCGGG GCCGGAGCCA GGGTATGCGC GCAGCTGTGG CAGCGCGTCC 
CCGCGCGGGT TCGCGCCCAG CCCCGGCTCA CAGCAGAGCG GCTACGGCGG CAGCCTCGGA 
GCTGGCCTCG GCGGCTACGG GGCCCCGGGC GTGACCGGCC TCGGTGTGCC CGGGTCCCCC 
AGCTTCCTCA ATGGCTCCAC GGCCACCTCA CCCTTCGCCA TCATGCCCTC TAGCCCCCCG 
CTGGCCTCTG CCTCCTCCAT GTCCCTCCCG GCGGCTGCCC CTACCACCAG CGTCTTCTCC 
TTCTCGCCTG TCCACATGAT CTCCGCTGTC AAACAGAGGA GCGCTTTTGC CCCCGTGCTG 
CGCCCGCCCA GCTCCCCACC CCAGGCCTGC CCCAGAACCC ACGGAGAGGG GCTTCCAGAC 
CAGCCTTTTG AAGATTCTGA CAAGTTTCAC TCTCCAGCTC GGGGACTTCA AGGCCTGGCA 
TACTCCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa46570
Clone ID Related Accession (Same CDS sequence) XM_025001086.1
Accession Version XM_025001086.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1716bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-06
Organism Bos taurus(cattle)
Product transcription factor COE4 isoform X7
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGCAGGGCG CGGGCATCCT GGACGCCAGT ACCGCGGCGC AGAGTGGTGT GGGCCTGGCA 
CGAGCACACT TTGAGAAGCA GCCCCCTTCC AACCTCAGGA AATCCAACTT CTTCCACTTC 
GTGCTGGCCA TGTATGACCG GCAGGGGCAG CCGGTGGAGG TGGAGCGCAC AGCCTTCATC 
GACTTTGTGG AGAAGGACCG AGAGCCTGGG GCTGAAAAGA CAAACAACGG AATCCATTAC 
CGCCTCCGGC TGGTATATAA CAACGGTCTT CGGACAGAAC AGGATCTCTA CGTGCGTCTC 
ATTGACTCCA TGTCCAAGCA GGCCATCATC TATGAAGGGC AGGACAAGAA CCCCGAAATG 
TGCCGTGTGC TGCTCACTCA TGAGATCATG TGCAGCCGAT GCTGTGACCG GAAGAGCTGT 
GGCAACCGGA ATGAGACGCC CTCAGACCCC GTCATCATTG ACAGGTTCTT CCTCAAGTTC 
TTCCTCAAAT GCAACCAGAA TTGCCTAAAG AACGCGGGGA ATCCCCGAGA CATGCGCCGC 
TTCCAGGTGG TGGTGTCCAC AACAGTGAGC GTGGACGGAC ACGTGCTGGC TGTGTCAGAC 
AACATGTTTG TGCACAACAA CTCCAAGCAT GGCCGCAGGG CACGCCGCCT GGACCCCTCC 
GAAGCTGCCA CCCCCTGCAT CAAGGCCATC AGTCCTGGGG AGGGCTGGAC CACAGGCGGC 
GCCACCGTCA TTGTCATCGG CGACAACTTC TTCGACGGGT TACAGGTCGT GTTTGGGAAC 
GTGCTCGTGT GGAGTGAGCT CATCACGCCC CACGCCATAC GGGTTCAGAC GCCCCCACGG 
CACATCCCTG GGGTGGTGGA GGTGACTCTG TCCTACAAAT CCAAGCAGTT TTGCAAGGGA 
GCCCCGGGAC GCTTTGTCTA CACAGCCCTG AATGAGCCCA CCATTGACTA CGGATTCCAG 
AGGCTGCAGA AAGTTATTCC CAGACACCCC GGAGACCCCG AGAGGCTGCC CAAGGAAGTG 
TTGCTGAAGC GGGCGGCCGA CTTGGCAGAG GCCCTGTACG GAGTGCCCAG CGGCAACCAG 
GAGCTCCTCC TGAAGCGCGC GGCGGACGTG GCCGAGGCTC TGTACAGCGC CCCCCGCGCG 
CCGGCGCCGC TAGGACCCCT GGCGCCCAGC CACCCGCACC CTGCCGTCGT GGGCATCAAC 
GCCTTCAGCA GCCCGCTGGC CATCGCCGTC GGGGACGCCA CGCCGGGGCC GGAGCCAGGG 
TATGCGCGCA GCTGTGGCAG CGCGTCCCCG CGCGGGTTCG CGCCCAGCCC CGGCTCACAG 
CAGAGCGGCT ACGGCGGCAG CCTCGGAGCT GGCCTCGGCG GCTACGGGGC CCCGGGCGTG 
ACCGGCCTCG GTGTGCCCGG GTCCCCCAGC TTCCTCAATG GCTCCACGGC CACCTCACCC 
TTCGCCATCA TGCCCTCTAG CCCCCCGCTG GCCTCTGCCT CCTCCATGTC CCTCCCGGCG 
GCTGCCCCTA CCACCAGCGT CTTCTCCTTC TCGCCTGTCC ACATGATCTC CGCTGTCAAA 
CAGAGGAGCG CTTTTGCCCC CGTGCTGCGC CCGCCCAGCT CCCCACCCCA GGCCTGCCCC 
AGAACCCACG GAGAGGGGCT TCCAGACCAG CCTTTTGAAG ATTCTGACAA GTTTCACTCT 
CCAGCTCGGG GACTTCAAGG CCTGGCATAC TCCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_024856854.1
CDS316..2031
Translation

Target ORF information:

RefSeq Version XM_025001086.1
Organism Bos taurus(cattle)
Definition Bos taurus early B cell factor 4 (EBF4), transcript variant X7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_025001086.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGCAGGGCG CGGGCATCCT GGACGCCAGT ACCGCGGCGC AGAGTGGTGT GGGCCTGGCA 
CGAGCACACT TTGAGAAGCA GCCCCCTTCC AACCTCAGGA AATCCAACTT CTTCCACTTC 
GTGCTGGCCA TGTATGACCG GCAGGGGCAG CCGGTGGAGG TGGAGCGCAC AGCCTTCATC 
GACTTTGTGG AGAAGGACCG AGAGCCTGGG GCTGAAAAGA CAAACAACGG AATCCATTAC 
CGCCTCCGGC TGGTATATAA CAACGGTCTT CGGACAGAAC AGGATCTCTA CGTGCGTCTC 
ATTGACTCCA TGTCCAAGCA GGCCATCATC TATGAAGGGC AGGACAAGAA CCCCGAAATG 
TGCCGTGTGC TGCTCACTCA TGAGATCATG TGCAGCCGAT GCTGTGACCG GAAGAGCTGT 
GGCAACCGGA ATGAGACGCC CTCAGACCCC GTCATCATTG ACAGGTTCTT CCTCAAGTTC 
TTCCTCAAAT GCAACCAGAA TTGCCTAAAG AACGCGGGGA ATCCCCGAGA CATGCGCCGC 
TTCCAGGTGG TGGTGTCCAC AACAGTGAGC GTGGACGGAC ACGTGCTGGC TGTGTCAGAC 
AACATGTTTG TGCACAACAA CTCCAAGCAT GGCCGCAGGG CACGCCGCCT GGACCCCTCC 
GAAGCTGCCA CCCCCTGCAT CAAGGCCATC AGTCCTGGGG AGGGCTGGAC CACAGGCGGC 
GCCACCGTCA TTGTCATCGG CGACAACTTC TTCGACGGGT TACAGGTCGT GTTTGGGAAC 
GTGCTCGTGT GGAGTGAGCT CATCACGCCC CACGCCATAC GGGTTCAGAC GCCCCCACGG 
CACATCCCTG GGGTGGTGGA GGTGACTCTG TCCTACAAAT CCAAGCAGTT TTGCAAGGGA 
GCCCCGGGAC GCTTTGTCTA CACAGCCCTG AATGAGCCCA CCATTGACTA CGGATTCCAG 
AGGCTGCAGA AAGTTATTCC CAGACACCCC GGAGACCCCG AGAGGCTGCC CAAGGAAGTG 
TTGCTGAAGC GGGCGGCCGA CTTGGCAGAG GCCCTGTACG GAGTGCCCAG CGGCAACCAG 
GAGCTCCTCC TGAAGCGCGC GGCGGACGTG GCCGAGGCTC TGTACAGCGC CCCCCGCGCG 
CCGGCGCCGC TAGGACCCCT GGCGCCCAGC CACCCGCACC CTGCCGTCGT GGGCATCAAC 
GCCTTCAGCA GCCCGCTGGC CATCGCCGTC GGGGACGCCA CGCCGGGGCC GGAGCCAGGG 
TATGCGCGCA GCTGTGGCAG CGCGTCCCCG CGCGGGTTCG CGCCCAGCCC CGGCTCACAG 
CAGAGCGGCT ACGGCGGCAG CCTCGGAGCT GGCCTCGGCG GCTACGGGGC CCCGGGCGTG 
ACCGGCCTCG GTGTGCCCGG GTCCCCCAGC TTCCTCAATG GCTCCACGGC CACCTCACCC 
TTCGCCATCA TGCCCTCTAG CCCCCCGCTG GCCTCTGCCT CCTCCATGTC CCTCCCGGCG 
GCTGCCCCTA CCACCAGCGT CTTCTCCTTC TCGCCTGTCC ACATGATCTC CGCTGTCAAA 
CAGAGGAGCG CTTTTGCCCC CGTGCTGCGC CCGCCCAGCT CCCCACCCCA GGCCTGCCCC 
AGAACCCACG GAGAGGGGCT TCCAGACCAG CCTTTTGAAG ATTCTGACAA GTTTCACTCT 
CCAGCTCGGG GACTTCAAGG CCTGGCATAC TCCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa92362
Clone ID Related Accession (Same CDS sequence) XM_015466076.1 , XM_025001085.1 , XM_015474216.1
Accession Version XM_015466076.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1731bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-25
Organism Bos taurus(cattle)
Product transcription factor COE4 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646145.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGGGGGTGT GCGGGAGCCG GCGAAGCGGC TGCAGGCTGA AGAGTGGGGA AGACATGAGT 
GGTGTGGGCC TGGCACGAGC ACACTTTGAG AAGCAGCCCC CTTCCAACCT CAGGAAATCC 
AACTTCTTCC ACTTCGTGCT GGCCATGTAT GACCGGCAGG GGCAGCCGGT GGAGGTGGAG 
CGCACAGCCT TCATCGACTT TGTGGAGAAG GACCGAGAGC CTGGGGCTGA AAAGACAAAC 
AACGGAATCC ATTACCGCCT CCGGCTGGTA TATAACAACG GTCTTCGGAC AGAACAGGAT 
CTCTACGTGC GTCTCATTGA CTCCATGTCC AAGCAGGCCA TCATCTATGA AGGGCAGGAC 
AAGAACCCCG AAATGTGCCG TGTGCTGCTC ACTCATGAGA TCATGTGCAG CCGATGCTGT 
GACCGGAAGA GCTGTGGCAA CCGGAATGAG ACGCCCTCAG ACCCCGTCAT CATTGACAGG 
TTCTTCCTCA AGTTCTTCCT CAAATGCAAC CAGAATTGCC TAAAGAACGC GGGGAATCCC 
CGAGACATGC GCCGCTTCCA GGTGGTGGTG TCCACAACAG TGAGCGTGGA CGGACACGTG 
CTGGCTGTGT CAGACAACAT GTTTGTGCAC AACAACTCCA AGCATGGCCG CAGGGCACGC 
CGCCTGGACC CCTCCGAAGC TGCCACCCCC TGCATCAAGG CCATCAGTCC TGGGGAGGGC 
TGGACCACAG GCGGCGCCAC CGTCATTGTC ATCGGCGACA ACTTCTTCGA CGGGTTACAG 
GTCGTGTTTG GGAACGTGCT CGTGTGGAGT GAGCTCATCA CGCCCCACGC CATACGGGTT 
CAGACGCCCC CACGGCACAT CCCTGGGGTG GTGGAGGTGA CTCTGTCCTA CAAATCCAAG 
CAGTTTTGCA AGGGAGCCCC GGGACGCTTT GTCTACACAG CCCTGAATGA GCCCACCATT 
GACTACGGAT TCCAGAGGCT GCAGAAAGTT ATTCCCAGAC ACCCCGGAGA CCCCGAGAGG 
CTGCCCAAGG AAGTGTTGCT GAAGCGGGCG GCCGACTTGG CAGAGGCCCT GTACGGAGTG 
CCCAGCGGCA ACCAGGAGCT CCTCCTGAAG CGCGCGGCGG ACGTGGCCGA GGCTCTGTAC 
AGCGCCCCCC GCGCGCCGGC GCCGCTAGGA CCCCTGGCGC CCAGCCACCC GCACCCTGCC 
GTCGTGGGCA TCAACGCCTT CAGCAGCCCG CTGGCCATCG CCGTCGGGGA CGCCACGCCG 
GGGCCGGAGC CAGGGTATGC GCGCAGCTGT GGCAGCGCGT CCCCGCGCGG GTTCGCGCCC 
AGCCCCGGCT CACAGCAGAG CGGCTACGGC GGCAGCCTCG GAGCTGGCCT CGGCGGCTAC 
GGGGCCCCGG GCGTGACCGG CCTCGGTGTG CCCGGGTCCC CCAGCTTCCT CAATGGCTCC 
ACGGCCACCT CACCCTTCGC CATCATGCCC TCTAGCCCCC CGCTGGCCTC TGCCTCCTCC 
ATGTCCCTCC CGGCGGCTGC CCCTACCACC AGCGTCTTCT CCTTCTCGCC TGTCCACATG 
ATCTCCGCTG TCAAACAGAG GAGCGCTTTT GCCCCCGTGC TGCGCCCGCC CAGCTCCCCA 
CCCCAGGCCT GCCCCAGAAC CCACGGAGAG GGGCTTCCAG ACCAGCCTTT TGAAGATTCT 
GACAAGTTTC ACTCTCCAGC TCGGGGACTT CAAGGCCTGG CATACTCCTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_015321562.1
CDS559..2289
Translation

Target ORF information:

RefSeq Version XM_015466076.1
Organism Bos taurus(cattle)
Definition Bos taurus early B-cell factor 4 (EBF4), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_015466076.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGGGGGTGT GCGGGAGCCG GCGAAGCGGC TGCAGGCTGA AGAGTGGGGA AGACATGAGT 
GGTGTGGGCC TGGCACGAGC ACACTTTGAG AAGCAGCCCC CTTCCAACCT CAGGAAATCC 
AACTTCTTCC ACTTCGTGCT GGCCATGTAT GACCGGCAGG GGCAGCCGGT GGAGGTGGAG 
CGCACAGCCT TCATCGACTT TGTGGAGAAG GACCGAGAGC CTGGGGCTGA AAAGACAAAC 
AACGGAATCC ATTACCGCCT CCGGCTGGTA TATAACAACG GTCTTCGGAC AGAACAGGAT 
CTCTACGTGC GTCTCATTGA CTCCATGTCC AAGCAGGCCA TCATCTATGA AGGGCAGGAC 
AAGAACCCCG AAATGTGCCG TGTGCTGCTC ACTCATGAGA TCATGTGCAG CCGATGCTGT 
GACCGGAAGA GCTGTGGCAA CCGGAATGAG ACGCCCTCAG ACCCCGTCAT CATTGACAGG 
TTCTTCCTCA AGTTCTTCCT CAAATGCAAC CAGAATTGCC TAAAGAACGC GGGGAATCCC 
CGAGACATGC GCCGCTTCCA GGTGGTGGTG TCCACAACAG TGAGCGTGGA CGGACACGTG 
CTGGCTGTGT CAGACAACAT GTTTGTGCAC AACAACTCCA AGCATGGCCG CAGGGCACGC 
CGCCTGGACC CCTCCGAAGC TGCCACCCCC TGCATCAAGG CCATCAGTCC TGGGGAGGGC 
TGGACCACAG GCGGCGCCAC CGTCATTGTC ATCGGCGACA ACTTCTTCGA CGGGTTACAG 
GTCGTGTTTG GGAACGTGCT CGTGTGGAGT GAGCTCATCA CGCCCCACGC CATACGGGTT 
CAGACGCCCC CACGGCACAT CCCTGGGGTG GTGGAGGTGA CTCTGTCCTA CAAATCCAAG 
CAGTTTTGCA AGGGAGCCCC GGGACGCTTT GTCTACACAG CCCTGAATGA GCCCACCATT 
GACTACGGAT TCCAGAGGCT GCAGAAAGTT ATTCCCAGAC ACCCCGGAGA CCCCGAGAGG 
CTGCCCAAGG AAGTGTTGCT GAAGCGGGCG GCCGACTTGG CAGAGGCCCT GTACGGAGTG 
CCCAGCGGCA ACCAGGAGCT CCTCCTGAAG CGCGCGGCGG ACGTGGCCGA GGCTCTGTAC 
AGCGCCCCCC GCGCGCCGGC GCCGCTAGGA CCCCTGGCGC CCAGCCACCC GCACCCTGCC 
GTCGTGGGCA TCAACGCCTT CAGCAGCCCG CTGGCCATCG CCGTCGGGGA CGCCACGCCG 
GGGCCGGAGC CAGGGTATGC GCGCAGCTGT GGCAGCGCGT CCCCGCGCGG GTTCGCGCCC 
AGCCCCGGCT CACAGCAGAG CGGCTACGGC GGCAGCCTCG GAGCTGGCCT CGGCGGCTAC 
GGGGCCCCGG GCGTGACCGG CCTCGGTGTG CCCGGGTCCC CCAGCTTCCT CAATGGCTCC 
ACGGCCACCT CACCCTTCGC CATCATGCCC TCTAGCCCCC CGCTGGCCTC TGCCTCCTCC 
ATGTCCCTCC CGGCGGCTGC CCCTACCACC AGCGTCTTCT CCTTCTCGCC TGTCCACATG 
ATCTCCGCTG TCAAACAGAG GAGCGCTTTT GCCCCCGTGC TGCGCCCGCC CAGCTCCCCA 
CCCCAGGCCT GCCCCAGAAC CCACGGAGAG GGGCTTCCAG ACCAGCCTTT TGAAGATTCT 
GACAAGTTTC ACTCTCCAGC TCGGGGACTT CAAGGCCTGG CATACTCCTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa92362
Clone ID Related Accession (Same CDS sequence) XM_015466076.1 , XM_025001085.1 , XM_015474216.1
Accession Version XM_025001085.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1731bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-06
Organism Bos taurus(cattle)
Product transcription factor COE4 isoform X6
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGGGGGTGT GCGGGAGCCG GCGAAGCGGC TGCAGGCTGA AGAGTGGGGA AGACATGAGT 
GGTGTGGGCC TGGCACGAGC ACACTTTGAG AAGCAGCCCC CTTCCAACCT CAGGAAATCC 
AACTTCTTCC ACTTCGTGCT GGCCATGTAT GACCGGCAGG GGCAGCCGGT GGAGGTGGAG 
CGCACAGCCT TCATCGACTT TGTGGAGAAG GACCGAGAGC CTGGGGCTGA AAAGACAAAC 
AACGGAATCC ATTACCGCCT CCGGCTGGTA TATAACAACG GTCTTCGGAC AGAACAGGAT 
CTCTACGTGC GTCTCATTGA CTCCATGTCC AAGCAGGCCA TCATCTATGA AGGGCAGGAC 
AAGAACCCCG AAATGTGCCG TGTGCTGCTC ACTCATGAGA TCATGTGCAG CCGATGCTGT 
GACCGGAAGA GCTGTGGCAA CCGGAATGAG ACGCCCTCAG ACCCCGTCAT CATTGACAGG 
TTCTTCCTCA AGTTCTTCCT CAAATGCAAC CAGAATTGCC TAAAGAACGC GGGGAATCCC 
CGAGACATGC GCCGCTTCCA GGTGGTGGTG TCCACAACAG TGAGCGTGGA CGGACACGTG 
CTGGCTGTGT CAGACAACAT GTTTGTGCAC AACAACTCCA AGCATGGCCG CAGGGCACGC 
CGCCTGGACC CCTCCGAAGC TGCCACCCCC TGCATCAAGG CCATCAGTCC TGGGGAGGGC 
TGGACCACAG GCGGCGCCAC CGTCATTGTC ATCGGCGACA ACTTCTTCGA CGGGTTACAG 
GTCGTGTTTG GGAACGTGCT CGTGTGGAGT GAGCTCATCA CGCCCCACGC CATACGGGTT 
CAGACGCCCC CACGGCACAT CCCTGGGGTG GTGGAGGTGA CTCTGTCCTA CAAATCCAAG 
CAGTTTTGCA AGGGAGCCCC GGGACGCTTT GTCTACACAG CCCTGAATGA GCCCACCATT 
GACTACGGAT TCCAGAGGCT GCAGAAAGTT ATTCCCAGAC ACCCCGGAGA CCCCGAGAGG 
CTGCCCAAGG AAGTGTTGCT GAAGCGGGCG GCCGACTTGG CAGAGGCCCT GTACGGAGTG 
CCCAGCGGCA ACCAGGAGCT CCTCCTGAAG CGCGCGGCGG ACGTGGCCGA GGCTCTGTAC 
AGCGCCCCCC GCGCGCCGGC GCCGCTAGGA CCCCTGGCGC CCAGCCACCC GCACCCTGCC 
GTCGTGGGCA TCAACGCCTT CAGCAGCCCG CTGGCCATCG CCGTCGGGGA CGCCACGCCG 
GGGCCGGAGC CAGGGTATGC GCGCAGCTGT GGCAGCGCGT CCCCGCGCGG GTTCGCGCCC 
AGCCCCGGCT CACAGCAGAG CGGCTACGGC GGCAGCCTCG GAGCTGGCCT CGGCGGCTAC 
GGGGCCCCGG GCGTGACCGG CCTCGGTGTG CCCGGGTCCC CCAGCTTCCT CAATGGCTCC 
ACGGCCACCT CACCCTTCGC CATCATGCCC TCTAGCCCCC CGCTGGCCTC TGCCTCCTCC 
ATGTCCCTCC CGGCGGCTGC CCCTACCACC AGCGTCTTCT CCTTCTCGCC TGTCCACATG 
ATCTCCGCTG TCAAACAGAG GAGCGCTTTT GCCCCCGTGC TGCGCCCGCC CAGCTCCCCA 
CCCCAGGCCT GCCCCAGAAC CCACGGAGAG GGGCTTCCAG ACCAGCCTTT TGAAGATTCT 
GACAAGTTTC ACTCTCCAGC TCGGGGACTT CAAGGCCTGG CATACTCCTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_024856853.1
CDS316..2046
Translation

Target ORF information:

RefSeq Version XM_025001085.1
Organism Bos taurus(cattle)
Definition Bos taurus early B cell factor 4 (EBF4), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_025001085.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGGGGGTGT GCGGGAGCCG GCGAAGCGGC TGCAGGCTGA AGAGTGGGGA AGACATGAGT 
GGTGTGGGCC TGGCACGAGC ACACTTTGAG AAGCAGCCCC CTTCCAACCT CAGGAAATCC 
AACTTCTTCC ACTTCGTGCT GGCCATGTAT GACCGGCAGG GGCAGCCGGT GGAGGTGGAG 
CGCACAGCCT TCATCGACTT TGTGGAGAAG GACCGAGAGC CTGGGGCTGA AAAGACAAAC 
AACGGAATCC ATTACCGCCT CCGGCTGGTA TATAACAACG GTCTTCGGAC AGAACAGGAT 
CTCTACGTGC GTCTCATTGA CTCCATGTCC AAGCAGGCCA TCATCTATGA AGGGCAGGAC 
AAGAACCCCG AAATGTGCCG TGTGCTGCTC ACTCATGAGA TCATGTGCAG CCGATGCTGT 
GACCGGAAGA GCTGTGGCAA CCGGAATGAG ACGCCCTCAG ACCCCGTCAT CATTGACAGG 
TTCTTCCTCA AGTTCTTCCT CAAATGCAAC CAGAATTGCC TAAAGAACGC GGGGAATCCC 
CGAGACATGC GCCGCTTCCA GGTGGTGGTG TCCACAACAG TGAGCGTGGA CGGACACGTG 
CTGGCTGTGT CAGACAACAT GTTTGTGCAC AACAACTCCA AGCATGGCCG CAGGGCACGC 
CGCCTGGACC CCTCCGAAGC TGCCACCCCC TGCATCAAGG CCATCAGTCC TGGGGAGGGC 
TGGACCACAG GCGGCGCCAC CGTCATTGTC ATCGGCGACA ACTTCTTCGA CGGGTTACAG 
GTCGTGTTTG GGAACGTGCT CGTGTGGAGT GAGCTCATCA CGCCCCACGC CATACGGGTT 
CAGACGCCCC CACGGCACAT CCCTGGGGTG GTGGAGGTGA CTCTGTCCTA CAAATCCAAG 
CAGTTTTGCA AGGGAGCCCC GGGACGCTTT GTCTACACAG CCCTGAATGA GCCCACCATT 
GACTACGGAT TCCAGAGGCT GCAGAAAGTT ATTCCCAGAC ACCCCGGAGA CCCCGAGAGG 
CTGCCCAAGG AAGTGTTGCT GAAGCGGGCG GCCGACTTGG CAGAGGCCCT GTACGGAGTG 
CCCAGCGGCA ACCAGGAGCT CCTCCTGAAG CGCGCGGCGG ACGTGGCCGA GGCTCTGTAC 
AGCGCCCCCC GCGCGCCGGC GCCGCTAGGA CCCCTGGCGC CCAGCCACCC GCACCCTGCC 
GTCGTGGGCA TCAACGCCTT CAGCAGCCCG CTGGCCATCG CCGTCGGGGA CGCCACGCCG 
GGGCCGGAGC CAGGGTATGC GCGCAGCTGT GGCAGCGCGT CCCCGCGCGG GTTCGCGCCC 
AGCCCCGGCT CACAGCAGAG CGGCTACGGC GGCAGCCTCG GAGCTGGCCT CGGCGGCTAC 
GGGGCCCCGG GCGTGACCGG CCTCGGTGTG CCCGGGTCCC CCAGCTTCCT CAATGGCTCC 
ACGGCCACCT CACCCTTCGC CATCATGCCC TCTAGCCCCC CGCTGGCCTC TGCCTCCTCC 
ATGTCCCTCC CGGCGGCTGC CCCTACCACC AGCGTCTTCT CCTTCTCGCC TGTCCACATG 
ATCTCCGCTG TCAAACAGAG GAGCGCTTTT GCCCCCGTGC TGCGCCCGCC CAGCTCCCCA 
CCCCAGGCCT GCCCCAGAAC CCACGGAGAG GGGCTTCCAG ACCAGCCTTT TGAAGATTCT 
GACAAGTTTC ACTCTCCAGC TCGGGGACTT CAAGGCCTGG CATACTCCTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa92362
Clone ID Related Accession (Same CDS sequence) XM_015466076.1 , XM_025001085.1 , XM_015474216.1
Accession Version XM_015474216.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1731bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-25
Organism Bos taurus(cattle)
Product transcription factor COE4 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104370.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGGGGGTGT GCGGGAGCCG GCGAAGCGGC TGCAGGCTGA AGAGTGGGGA AGACATGAGT 
GGTGTGGGCC TGGCACGAGC ACACTTTGAG AAGCAGCCCC CTTCCAACCT CAGGAAATCC 
AACTTCTTCC ACTTCGTGCT GGCCATGTAT GACCGGCAGG GGCAGCCGGT GGAGGTGGAG 
CGCACAGCCT TCATCGACTT TGTGGAGAAG GACCGAGAGC CTGGGGCTGA AAAGACAAAC 
AACGGAATCC ATTACCGCCT CCGGCTGGTA TATAACAACG GTCTTCGGAC AGAACAGGAT 
CTCTACGTGC GTCTCATTGA CTCCATGTCC AAGCAGGCCA TCATCTATGA AGGGCAGGAC 
AAGAACCCCG AAATGTGCCG TGTGCTGCTC ACTCATGAGA TCATGTGCAG CCGATGCTGT 
GACCGGAAGA GCTGTGGCAA CCGGAATGAG ACGCCCTCAG ACCCCGTCAT CATTGACAGG 
TTCTTCCTCA AGTTCTTCCT CAAATGCAAC CAGAATTGCC TAAAGAACGC GGGGAATCCC 
CGAGACATGC GCCGCTTCCA GGTGGTGGTG TCCACAACAG TGAGCGTGGA CGGACACGTG 
CTGGCTGTGT CAGACAACAT GTTTGTGCAC AACAACTCCA AGCATGGCCG CAGGGCACGC 
CGCCTGGACC CCTCCGAAGC TGCCACCCCC TGCATCAAGG CCATCAGTCC TGGGGAGGGC 
TGGACCACAG GCGGCGCCAC CGTCATTGTC ATCGGCGACA ACTTCTTCGA CGGGTTACAG 
GTCGTGTTTG GGAACGTGCT CGTGTGGAGT GAGCTCATCA CGCCCCACGC CATACGGGTT 
CAGACGCCCC CACGGCACAT CCCTGGGGTG GTGGAGGTGA CTCTGTCCTA CAAATCCAAG 
CAGTTTTGCA AGGGAGCCCC GGGACGCTTT GTCTACACAG CCCTGAATGA GCCCACCATT 
GACTACGGAT TCCAGAGGCT GCAGAAAGTT ATTCCCAGAC ACCCCGGAGA CCCCGAGAGG 
CTGCCCAAGG AAGTGTTGCT GAAGCGGGCG GCCGACTTGG CAGAGGCCCT GTACGGAGTG 
CCCAGCGGCA ACCAGGAGCT CCTCCTGAAG CGCGCGGCGG ACGTGGCCGA GGCTCTGTAC 
AGCGCCCCCC GCGCGCCGGC GCCGCTAGGA CCCCTGGCGC CCAGCCACCC GCACCCTGCC 
GTCGTGGGCA TCAACGCCTT CAGCAGCCCG CTGGCCATCG CCGTCGGGGA CGCCACGCCG 
GGGCCGGAGC CAGGGTATGC GCGCAGCTGT GGCAGCGCGT CCCCGCGCGG GTTCGCGCCC 
AGCCCCGGCT CACAGCAGAG CGGCTACGGC GGCAGCCTCG GAGCTGGCCT CGGCGGCTAC 
GGGGCCCCGG GCGTGACCGG CCTCGGTGTG CCCGGGTCCC CCAGCTTCCT CAATGGCTCC 
ACGGCCACCT CACCCTTCGC CATCATGCCC TCTAGCCCCC CGCTGGCCTC TGCCTCCTCC 
ATGTCCCTCC CGGCGGCTGC CCCTACCACC AGCGTCTTCT CCTTCTCGCC TGTCCACATG 
ATCTCCGCTG TCAAACAGAG GAGCGCTTTT GCCCCCGTGC TGCGCCCGCC CAGCTCCCCA 
CCCCAGGCCT GCCCCAGAAC CCACGGAGAG GGGCTTCCAG ACCAGCCTTT TGAAGATTCT 
GACAAGTTTC ACTCTCCAGC TCGGGGACTT CAAGGCCTGG CATACTCCTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_015329702.1
CDS559..2289
Translation

Target ORF information:

RefSeq Version XM_015474216.1
Organism Bos taurus(cattle)
Definition Bos taurus early B-cell factor 4 (EBF4), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_015474216.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGGGGGTGT GCGGGAGCCG GCGAAGCGGC TGCAGGCTGA AGAGTGGGGA AGACATGAGT 
GGTGTGGGCC TGGCACGAGC ACACTTTGAG AAGCAGCCCC CTTCCAACCT CAGGAAATCC 
AACTTCTTCC ACTTCGTGCT GGCCATGTAT GACCGGCAGG GGCAGCCGGT GGAGGTGGAG 
CGCACAGCCT TCATCGACTT TGTGGAGAAG GACCGAGAGC CTGGGGCTGA AAAGACAAAC 
AACGGAATCC ATTACCGCCT CCGGCTGGTA TATAACAACG GTCTTCGGAC AGAACAGGAT 
CTCTACGTGC GTCTCATTGA CTCCATGTCC AAGCAGGCCA TCATCTATGA AGGGCAGGAC 
AAGAACCCCG AAATGTGCCG TGTGCTGCTC ACTCATGAGA TCATGTGCAG CCGATGCTGT 
GACCGGAAGA GCTGTGGCAA CCGGAATGAG ACGCCCTCAG ACCCCGTCAT CATTGACAGG 
TTCTTCCTCA AGTTCTTCCT CAAATGCAAC CAGAATTGCC TAAAGAACGC GGGGAATCCC 
CGAGACATGC GCCGCTTCCA GGTGGTGGTG TCCACAACAG TGAGCGTGGA CGGACACGTG 
CTGGCTGTGT CAGACAACAT GTTTGTGCAC AACAACTCCA AGCATGGCCG CAGGGCACGC 
CGCCTGGACC CCTCCGAAGC TGCCACCCCC TGCATCAAGG CCATCAGTCC TGGGGAGGGC 
TGGACCACAG GCGGCGCCAC CGTCATTGTC ATCGGCGACA ACTTCTTCGA CGGGTTACAG 
GTCGTGTTTG GGAACGTGCT CGTGTGGAGT GAGCTCATCA CGCCCCACGC CATACGGGTT 
CAGACGCCCC CACGGCACAT CCCTGGGGTG GTGGAGGTGA CTCTGTCCTA CAAATCCAAG 
CAGTTTTGCA AGGGAGCCCC GGGACGCTTT GTCTACACAG CCCTGAATGA GCCCACCATT 
GACTACGGAT TCCAGAGGCT GCAGAAAGTT ATTCCCAGAC ACCCCGGAGA CCCCGAGAGG 
CTGCCCAAGG AAGTGTTGCT GAAGCGGGCG GCCGACTTGG CAGAGGCCCT GTACGGAGTG 
CCCAGCGGCA ACCAGGAGCT CCTCCTGAAG CGCGCGGCGG ACGTGGCCGA GGCTCTGTAC 
AGCGCCCCCC GCGCGCCGGC GCCGCTAGGA CCCCTGGCGC CCAGCCACCC GCACCCTGCC 
GTCGTGGGCA TCAACGCCTT CAGCAGCCCG CTGGCCATCG CCGTCGGGGA CGCCACGCCG 
GGGCCGGAGC CAGGGTATGC GCGCAGCTGT GGCAGCGCGT CCCCGCGCGG GTTCGCGCCC 
AGCCCCGGCT CACAGCAGAG CGGCTACGGC GGCAGCCTCG GAGCTGGCCT CGGCGGCTAC 
GGGGCCCCGG GCGTGACCGG CCTCGGTGTG CCCGGGTCCC CCAGCTTCCT CAATGGCTCC 
ACGGCCACCT CACCCTTCGC CATCATGCCC TCTAGCCCCC CGCTGGCCTC TGCCTCCTCC 
ATGTCCCTCC CGGCGGCTGC CCCTACCACC AGCGTCTTCT CCTTCTCGCC TGTCCACATG 
ATCTCCGCTG TCAAACAGAG GAGCGCTTTT GCCCCCGTGC TGCGCCCGCC CAGCTCCCCA 
CCCCAGGCCT GCCCCAGAAC CCACGGAGAG GGGCTTCCAG ACCAGCCTTT TGAAGATTCT 
GACAAGTTTC ACTCTCCAGC TCGGGGACTT CAAGGCCTGG CATACTCCTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa92363
Clone ID Related Accession (Same CDS sequence) XM_015474217.1
Accession Version XM_015474217.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1731bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-25
Organism Bos taurus(cattle)
Product transcription factor COE4 isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104370.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGCAGGGCG CGGGCATCCT GGACGCCAGT ACCGCGGCGC AGAGCTTTCC TCCCCACAGT 
GGTGTGGGCC TGGCACGAGC ACACTTTGAG AAGCAGCCCC CTTCCAACCT CAGGAAATCC 
AACTTCTTCC ACTTCGTGCT GGCCATGTAT GACCGGCAGG GGCAGCCGGT GGAGGTGGAG 
CGCACAGCCT TCATCGACTT TGTGGAGAAG GACCGAGAGC CTGGGGCTGA AAAGACAAAC 
AACGGAATCC ATTACCGCCT CCGGCTGGTA TATAACAACG GTCTTCGGAC AGAACAGGAT 
CTCTACGTGC GTCTCATTGA CTCCATGTCC AAGCAGGCCA TCATCTATGA AGGGCAGGAC 
AAGAACCCCG AAATGTGCCG TGTGCTGCTC ACTCATGAGA TCATGTGCAG CCGATGCTGT 
GACCGGAAGA GCTGTGGCAA CCGGAATGAG ACGCCCTCAG ACCCCGTCAT CATTGACAGG 
TTCTTCCTCA AGTTCTTCCT CAAATGCAAC CAGAATTGCC TAAAGAACGC GGGGAATCCC 
CGAGACATGC GCCGCTTCCA GGTGGTGGTG TCCACAACAG TGAGCGTGGA CGGACACGTG 
CTGGCTGTGT CAGACAACAT GTTTGTGCAC AACAACTCCA AGCATGGCCG CAGGGCACGC 
CGCCTGGACC CCTCCGAAGC TGCCACCCCC TGCATCAAGG CCATCAGTCC TGGGGAGGGC 
TGGACCACAG GCGGCGCCAC CGTCATTGTC ATCGGCGACA ACTTCTTCGA CGGGTTACAG 
GTCGTGTTTG GGAACGTGCT CGTGTGGAGT GAGCTCATCA CGCCCCACGC CATACGGGTT 
CAGACGCCCC CACGGCACAT CCCTGGGGTG GTGGAGGTGA CTCTGTCCTA CAAATCCAAG 
CAGTTTTGCA AGGGAGCCCC GGGACGCTTT GTCTACACAG CCCTGAATGA GCCCACCATT 
GACTACGGAT TCCAGAGGCT GCAGAAAGTT ATTCCCAGAC ACCCCGGAGA CCCCGAGAGG 
CTGCCCAAGG AAGTGTTGCT GAAGCGGGCG GCCGACTTGG CAGAGGCCCT GTACGGAGTG 
CCCAGCGGCA ACCAGGAGCT CCTCCTGAAG CGCGCGGCGG ACGTGGCCGA GGCTCTGTAC 
AGCGCCCCCC GCGCGCCGGC GCCGCTAGGA CCCCTGGCGC CCAGCCACCC GCACCCTGCC 
GTCGTGGGCA TCAACGCCTT CAGCAGCCCG CTGGCCATCG CCGTCGGGGA CGCCACGCCG 
GGGCCGGAGC CAGGGTATGC GCGCAGCTGT GGCAGCGCGT CCCCGCGCGG GTTCGCGCCC 
AGCCCCGGCT CACAGCAGAG CGGCTACGGC GGCAGCCTCG GAGCTGGCCT CGGCGGCTAC 
GGGGCCCCGG GCGTGACCGG CCTCGGTGTG CCCGGGTCCC CCAGCTTCCT CAATGGCTCC 
ACGGCCACCT CACCCTTCGC CATCATGCCC TCTAGCCCCC CGCTGGCCTC TGCCTCCTCC 
ATGTCCCTCC CGGCGGCTGC CCCTACCACC AGCGTCTTCT CCTTCTCGCC TGTCCACATG 
ATCTCCGCTG TCAAACAGAG GAGCGCTTTT GCCCCCGTGC TGCGCCCGCC CAGCTCCCCA 
CCCCAGGCCT GCCCCAGAAC CCACGGAGAG GGGCTTCCAG ACCAGCCTTT TGAAGATTCT 
GACAAGTTTC ACTCTCCAGC TCGGGGACTT CAAGGCCTGG CATACTCCTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_015329703.1
CDS323..2053
Translation

Target ORF information:

RefSeq Version XM_015474217.1
Organism Bos taurus(cattle)
Definition Bos taurus early B-cell factor 4 (EBF4), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_015474217.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGCAGGGCG CGGGCATCCT GGACGCCAGT ACCGCGGCGC AGAGCTTTCC TCCCCACAGT 
GGTGTGGGCC TGGCACGAGC ACACTTTGAG AAGCAGCCCC CTTCCAACCT CAGGAAATCC 
AACTTCTTCC ACTTCGTGCT GGCCATGTAT GACCGGCAGG GGCAGCCGGT GGAGGTGGAG 
CGCACAGCCT TCATCGACTT TGTGGAGAAG GACCGAGAGC CTGGGGCTGA AAAGACAAAC 
AACGGAATCC ATTACCGCCT CCGGCTGGTA TATAACAACG GTCTTCGGAC AGAACAGGAT 
CTCTACGTGC GTCTCATTGA CTCCATGTCC AAGCAGGCCA TCATCTATGA AGGGCAGGAC 
AAGAACCCCG AAATGTGCCG TGTGCTGCTC ACTCATGAGA TCATGTGCAG CCGATGCTGT 
GACCGGAAGA GCTGTGGCAA CCGGAATGAG ACGCCCTCAG ACCCCGTCAT CATTGACAGG 
TTCTTCCTCA AGTTCTTCCT CAAATGCAAC CAGAATTGCC TAAAGAACGC GGGGAATCCC 
CGAGACATGC GCCGCTTCCA GGTGGTGGTG TCCACAACAG TGAGCGTGGA CGGACACGTG 
CTGGCTGTGT CAGACAACAT GTTTGTGCAC AACAACTCCA AGCATGGCCG CAGGGCACGC 
CGCCTGGACC CCTCCGAAGC TGCCACCCCC TGCATCAAGG CCATCAGTCC TGGGGAGGGC 
TGGACCACAG GCGGCGCCAC CGTCATTGTC ATCGGCGACA ACTTCTTCGA CGGGTTACAG 
GTCGTGTTTG GGAACGTGCT CGTGTGGAGT GAGCTCATCA CGCCCCACGC CATACGGGTT 
CAGACGCCCC CACGGCACAT CCCTGGGGTG GTGGAGGTGA CTCTGTCCTA CAAATCCAAG 
CAGTTTTGCA AGGGAGCCCC GGGACGCTTT GTCTACACAG CCCTGAATGA GCCCACCATT 
GACTACGGAT TCCAGAGGCT GCAGAAAGTT ATTCCCAGAC ACCCCGGAGA CCCCGAGAGG 
CTGCCCAAGG AAGTGTTGCT GAAGCGGGCG GCCGACTTGG CAGAGGCCCT GTACGGAGTG 
CCCAGCGGCA ACCAGGAGCT CCTCCTGAAG CGCGCGGCGG ACGTGGCCGA GGCTCTGTAC 
AGCGCCCCCC GCGCGCCGGC GCCGCTAGGA CCCCTGGCGC CCAGCCACCC GCACCCTGCC 
GTCGTGGGCA TCAACGCCTT CAGCAGCCCG CTGGCCATCG CCGTCGGGGA CGCCACGCCG 
GGGCCGGAGC CAGGGTATGC GCGCAGCTGT GGCAGCGCGT CCCCGCGCGG GTTCGCGCCC 
AGCCCCGGCT CACAGCAGAG CGGCTACGGC GGCAGCCTCG GAGCTGGCCT CGGCGGCTAC 
GGGGCCCCGG GCGTGACCGG CCTCGGTGTG CCCGGGTCCC CCAGCTTCCT CAATGGCTCC 
ACGGCCACCT CACCCTTCGC CATCATGCCC TCTAGCCCCC CGCTGGCCTC TGCCTCCTCC 
ATGTCCCTCC CGGCGGCTGC CCCTACCACC AGCGTCTTCT CCTTCTCGCC TGTCCACATG 
ATCTCCGCTG TCAAACAGAG GAGCGCTTTT GCCCCCGTGC TGCGCCCGCC CAGCTCCCCA 
CCCCAGGCCT GCCCCAGAAC CCACGGAGAG GGGCTTCCAG ACCAGCCTTT TGAAGATTCT 
GACAAGTTTC ACTCTCCAGC TCGGGGACTT CAAGGCCTGG CATACTCCTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa94802
Clone ID Related Accession (Same CDS sequence) XM_025001082.1 , XM_015466077.1
Accession Version XM_025001082.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1812bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-06
Organism Bos taurus(cattle)
Product transcription factor COE4 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGGACGCTG TGCCCCGCGG CGGGCTGAAC CTGAAGGAGG AGCCGCTGCT GCCCGCGGGC 
CTGGGCTCGG TGCGCTCCTG GATGCAGGGC GCGGGCATCC TGGACGCCAG TACCGCGGCG 
CAGAGCTTTC CTCCCCACAG TGGTGTGGGC CTGGCACGAG CACACTTTGA GAAGCAGCCC 
CCTTCCAACC TCAGGAAATC CAACTTCTTC CACTTCGTGC TGGCCATGTA TGACCGGCAG 
GGGCAGCCGG TGGAGGTGGA GCGCACAGCC TTCATCGACT TTGTGGAGAA GGACCGAGAG 
CCTGGGGCTG AAAAGACAAA CAACGGAATC CATTACCGCC TCCGGCTGGT ATATAACAAC 
GGTCTTCGGA CAGAACAGGA TCTCTACGTG CGTCTCATTG ACTCCATGTC CAAGCAGGCC 
ATCATCTATG AAGGGCAGGA CAAGAACCCC GAAATGTGCC GTGTGCTGCT CACTCATGAG 
ATCATGTGCA GCCGATGCTG TGACCGGAAG AGCTGTGGCA ACCGGAATGA GACGCCCTCA 
GACCCCGTCA TCATTGACAG GTTCTTCCTC AAGTTCTTCC TCAAATGCAA CCAGAATTGC 
CTAAAGAACG CGGGGAATCC CCGAGACATG CGCCGCTTCC AGGTGGTGGT GTCCACAACA 
GTGAGCGTGG ACGGACACGT GCTGGCTGTG TCAGACAACA TGTTTGTGCA CAACAACTCC 
AAGCATGGCC GCAGGGCACG CCGCCTGGAC CCCTCCGAAG CTGCCACCCC CTGCATCAAG 
GCCATCAGTC CTGGGGAGGG CTGGACCACA GGCGGCGCCA CCGTCATTGT CATCGGCGAC 
AACTTCTTCG ACGGGTTACA GGTCGTGTTT GGGAACGTGC TCGTGTGGAG TGAGCTCATC 
ACGCCCCACG CCATACGGGT TCAGACGCCC CCACGGCACA TCCCTGGGGT GGTGGAGGTG 
ACTCTGTCCT ACAAATCCAA GCAGTTTTGC AAGGGAGCCC CGGGACGCTT TGTCTACACA 
GCCCTGAATG AGCCCACCAT TGACTACGGA TTCCAGAGGC TGCAGAAAGT TATTCCCAGA 
CACCCCGGAG ACCCCGAGAG GCTGCCCAAG GAAGTGTTGC TGAAGCGGGC GGCCGACTTG 
GCAGAGGCCC TGTACGGAGT GCCCAGCGGC AACCAGGAGC TCCTCCTGAA GCGCGCGGCG 
GACGTGGCCG AGGCTCTGTA CAGCGCCCCC CGCGCGCCGG CGCCGCTAGG ACCCCTGGCG 
CCCAGCCACC CGCACCCTGC CGTCGTGGGC ATCAACGCCT TCAGCAGCCC GCTGGCCATC 
GCCGTCGGGG ACGCCACGCC GGGGCCGGAG CCAGGGTATG CGCGCAGCTG TGGCAGCGCG 
TCCCCGCGCG GGTTCGCGCC CAGCCCCGGC TCACAGCAGA GCGGCTACGG CGGCAGCCTC 
GGAGCTGGCC TCGGCGGCTA CGGGGCCCCG GGCGTGACCG GCCTCGGTGT GCCCGGGTCC 
CCCAGCTTCC TCAATGGCTC CACGGCCACC TCACCCTTCG CCATCATGCC CTCTAGCCCC 
CCGCTGGCCT CTGCCTCCTC CATGTCCCTC CCGGCGGCTG CCCCTACCAC CAGCGTCTTC 
TCCTTCTCGC CTGTCCACAT GATCTCCGCT GTCAAACAGA GGAGCGCTTT TGCCCCCGTG 
CTGCGCCCGC CCAGCTCCCC ACCCCAGGCC TGCCCCAGAA CCCACGGAGA GGGGCTTCCA 
GACCAGCCTT TTGAAGATTC TGACAAGTTT CACTCTCCAG CTCGGGGACT TCAAGGCCTG 
GCATACTCCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_024856850.1
CDS240..2051
Translation

Target ORF information:

RefSeq Version XM_025001082.1
Organism Bos taurus(cattle)
Definition Bos taurus early B cell factor 4 (EBF4), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_025001082.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGGACGCTG TGCCCCGCGG CGGGCTGAAC CTGAAGGAGG AGCCGCTGCT GCCCGCGGGC 
CTGGGCTCGG TGCGCTCCTG GATGCAGGGC GCGGGCATCC TGGACGCCAG TACCGCGGCG 
CAGAGCTTTC CTCCCCACAG TGGTGTGGGC CTGGCACGAG CACACTTTGA GAAGCAGCCC 
CCTTCCAACC TCAGGAAATC CAACTTCTTC CACTTCGTGC TGGCCATGTA TGACCGGCAG 
GGGCAGCCGG TGGAGGTGGA GCGCACAGCC TTCATCGACT TTGTGGAGAA GGACCGAGAG 
CCTGGGGCTG AAAAGACAAA CAACGGAATC CATTACCGCC TCCGGCTGGT ATATAACAAC 
GGTCTTCGGA CAGAACAGGA TCTCTACGTG CGTCTCATTG ACTCCATGTC CAAGCAGGCC 
ATCATCTATG AAGGGCAGGA CAAGAACCCC GAAATGTGCC GTGTGCTGCT CACTCATGAG 
ATCATGTGCA GCCGATGCTG TGACCGGAAG AGCTGTGGCA ACCGGAATGA GACGCCCTCA 
GACCCCGTCA TCATTGACAG GTTCTTCCTC AAGTTCTTCC TCAAATGCAA CCAGAATTGC 
CTAAAGAACG CGGGGAATCC CCGAGACATG CGCCGCTTCC AGGTGGTGGT GTCCACAACA 
GTGAGCGTGG ACGGACACGT GCTGGCTGTG TCAGACAACA TGTTTGTGCA CAACAACTCC 
AAGCATGGCC GCAGGGCACG CCGCCTGGAC CCCTCCGAAG CTGCCACCCC CTGCATCAAG 
GCCATCAGTC CTGGGGAGGG CTGGACCACA GGCGGCGCCA CCGTCATTGT CATCGGCGAC 
AACTTCTTCG ACGGGTTACA GGTCGTGTTT GGGAACGTGC TCGTGTGGAG TGAGCTCATC 
ACGCCCCACG CCATACGGGT TCAGACGCCC CCACGGCACA TCCCTGGGGT GGTGGAGGTG 
ACTCTGTCCT ACAAATCCAA GCAGTTTTGC AAGGGAGCCC CGGGACGCTT TGTCTACACA 
GCCCTGAATG AGCCCACCAT TGACTACGGA TTCCAGAGGC TGCAGAAAGT TATTCCCAGA 
CACCCCGGAG ACCCCGAGAG GCTGCCCAAG GAAGTGTTGC TGAAGCGGGC GGCCGACTTG 
GCAGAGGCCC TGTACGGAGT GCCCAGCGGC AACCAGGAGC TCCTCCTGAA GCGCGCGGCG 
GACGTGGCCG AGGCTCTGTA CAGCGCCCCC CGCGCGCCGG CGCCGCTAGG ACCCCTGGCG 
CCCAGCCACC CGCACCCTGC CGTCGTGGGC ATCAACGCCT TCAGCAGCCC GCTGGCCATC 
GCCGTCGGGG ACGCCACGCC GGGGCCGGAG CCAGGGTATG CGCGCAGCTG TGGCAGCGCG 
TCCCCGCGCG GGTTCGCGCC CAGCCCCGGC TCACAGCAGA GCGGCTACGG CGGCAGCCTC 
GGAGCTGGCC TCGGCGGCTA CGGGGCCCCG GGCGTGACCG GCCTCGGTGT GCCCGGGTCC 
CCCAGCTTCC TCAATGGCTC CACGGCCACC TCACCCTTCG CCATCATGCC CTCTAGCCCC 
CCGCTGGCCT CTGCCTCCTC CATGTCCCTC CCGGCGGCTG CCCCTACCAC CAGCGTCTTC 
TCCTTCTCGC CTGTCCACAT GATCTCCGCT GTCAAACAGA GGAGCGCTTT TGCCCCCGTG 
CTGCGCCCGC CCAGCTCCCC ACCCCAGGCC TGCCCCAGAA CCCACGGAGA GGGGCTTCCA 
GACCAGCCTT TTGAAGATTC TGACAAGTTT CACTCTCCAG CTCGGGGACT TCAAGGCCTG 
GCATACTCCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa94802
Clone ID Related Accession (Same CDS sequence) XM_025001082.1 , XM_015466077.1
Accession Version XM_015466077.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1812bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-25
Organism Bos taurus(cattle)
Product transcription factor COE4 isoform X4
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646145.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGGACGCTG TGCCCCGCGG CGGGCTGAAC CTGAAGGAGG AGCCGCTGCT GCCCGCGGGC 
CTGGGCTCGG TGCGCTCCTG GATGCAGGGC GCGGGCATCC TGGACGCCAG TACCGCGGCG 
CAGAGCTTTC CTCCCCACAG TGGTGTGGGC CTGGCACGAG CACACTTTGA GAAGCAGCCC 
CCTTCCAACC TCAGGAAATC CAACTTCTTC CACTTCGTGC TGGCCATGTA TGACCGGCAG 
GGGCAGCCGG TGGAGGTGGA GCGCACAGCC TTCATCGACT TTGTGGAGAA GGACCGAGAG 
CCTGGGGCTG AAAAGACAAA CAACGGAATC CATTACCGCC TCCGGCTGGT ATATAACAAC 
GGTCTTCGGA CAGAACAGGA TCTCTACGTG CGTCTCATTG ACTCCATGTC CAAGCAGGCC 
ATCATCTATG AAGGGCAGGA CAAGAACCCC GAAATGTGCC GTGTGCTGCT CACTCATGAG 
ATCATGTGCA GCCGATGCTG TGACCGGAAG AGCTGTGGCA ACCGGAATGA GACGCCCTCA 
GACCCCGTCA TCATTGACAG GTTCTTCCTC AAGTTCTTCC TCAAATGCAA CCAGAATTGC 
CTAAAGAACG CGGGGAATCC CCGAGACATG CGCCGCTTCC AGGTGGTGGT GTCCACAACA 
GTGAGCGTGG ACGGACACGT GCTGGCTGTG TCAGACAACA TGTTTGTGCA CAACAACTCC 
AAGCATGGCC GCAGGGCACG CCGCCTGGAC CCCTCCGAAG CTGCCACCCC CTGCATCAAG 
GCCATCAGTC CTGGGGAGGG CTGGACCACA GGCGGCGCCA CCGTCATTGT CATCGGCGAC 
AACTTCTTCG ACGGGTTACA GGTCGTGTTT GGGAACGTGC TCGTGTGGAG TGAGCTCATC 
ACGCCCCACG CCATACGGGT TCAGACGCCC CCACGGCACA TCCCTGGGGT GGTGGAGGTG 
ACTCTGTCCT ACAAATCCAA GCAGTTTTGC AAGGGAGCCC CGGGACGCTT TGTCTACACA 
GCCCTGAATG AGCCCACCAT TGACTACGGA TTCCAGAGGC TGCAGAAAGT TATTCCCAGA 
CACCCCGGAG ACCCCGAGAG GCTGCCCAAG GAAGTGTTGC TGAAGCGGGC GGCCGACTTG 
GCAGAGGCCC TGTACGGAGT GCCCAGCGGC AACCAGGAGC TCCTCCTGAA GCGCGCGGCG 
GACGTGGCCG AGGCTCTGTA CAGCGCCCCC CGCGCGCCGG CGCCGCTAGG ACCCCTGGCG 
CCCAGCCACC CGCACCCTGC CGTCGTGGGC ATCAACGCCT TCAGCAGCCC GCTGGCCATC 
GCCGTCGGGG ACGCCACGCC GGGGCCGGAG CCAGGGTATG CGCGCAGCTG TGGCAGCGCG 
TCCCCGCGCG GGTTCGCGCC CAGCCCCGGC TCACAGCAGA GCGGCTACGG CGGCAGCCTC 
GGAGCTGGCC TCGGCGGCTA CGGGGCCCCG GGCGTGACCG GCCTCGGTGT GCCCGGGTCC 
CCCAGCTTCC TCAATGGCTC CACGGCCACC TCACCCTTCG CCATCATGCC CTCTAGCCCC 
CCGCTGGCCT CTGCCTCCTC CATGTCCCTC CCGGCGGCTG CCCCTACCAC CAGCGTCTTC 
TCCTTCTCGC CTGTCCACAT GATCTCCGCT GTCAAACAGA GGAGCGCTTT TGCCCCCGTG 
CTGCGCCCGC CCAGCTCCCC ACCCCAGGCC TGCCCCAGAA CCCACGGAGA GGGGCTTCCA 
GACCAGCCTT TTGAAGATTC TGACAAGTTT CACTCTCCAG CTCGGGGACT TCAAGGCCTG 
GCATACTCCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_015321563.1
CDS225..2036
Translation

Target ORF information:

RefSeq Version XM_015466077.1
Organism Bos taurus(cattle)
Definition Bos taurus early B-cell factor 4 (EBF4), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_015466077.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGGACGCTG TGCCCCGCGG CGGGCTGAAC CTGAAGGAGG AGCCGCTGCT GCCCGCGGGC 
CTGGGCTCGG TGCGCTCCTG GATGCAGGGC GCGGGCATCC TGGACGCCAG TACCGCGGCG 
CAGAGCTTTC CTCCCCACAG TGGTGTGGGC CTGGCACGAG CACACTTTGA GAAGCAGCCC 
CCTTCCAACC TCAGGAAATC CAACTTCTTC CACTTCGTGC TGGCCATGTA TGACCGGCAG 
GGGCAGCCGG TGGAGGTGGA GCGCACAGCC TTCATCGACT TTGTGGAGAA GGACCGAGAG 
CCTGGGGCTG AAAAGACAAA CAACGGAATC CATTACCGCC TCCGGCTGGT ATATAACAAC 
GGTCTTCGGA CAGAACAGGA TCTCTACGTG CGTCTCATTG ACTCCATGTC CAAGCAGGCC 
ATCATCTATG AAGGGCAGGA CAAGAACCCC GAAATGTGCC GTGTGCTGCT CACTCATGAG 
ATCATGTGCA GCCGATGCTG TGACCGGAAG AGCTGTGGCA ACCGGAATGA GACGCCCTCA 
GACCCCGTCA TCATTGACAG GTTCTTCCTC AAGTTCTTCC TCAAATGCAA CCAGAATTGC 
CTAAAGAACG CGGGGAATCC CCGAGACATG CGCCGCTTCC AGGTGGTGGT GTCCACAACA 
GTGAGCGTGG ACGGACACGT GCTGGCTGTG TCAGACAACA TGTTTGTGCA CAACAACTCC 
AAGCATGGCC GCAGGGCACG CCGCCTGGAC CCCTCCGAAG CTGCCACCCC CTGCATCAAG 
GCCATCAGTC CTGGGGAGGG CTGGACCACA GGCGGCGCCA CCGTCATTGT CATCGGCGAC 
AACTTCTTCG ACGGGTTACA GGTCGTGTTT GGGAACGTGC TCGTGTGGAG TGAGCTCATC 
ACGCCCCACG CCATACGGGT TCAGACGCCC CCACGGCACA TCCCTGGGGT GGTGGAGGTG 
ACTCTGTCCT ACAAATCCAA GCAGTTTTGC AAGGGAGCCC CGGGACGCTT TGTCTACACA 
GCCCTGAATG AGCCCACCAT TGACTACGGA TTCCAGAGGC TGCAGAAAGT TATTCCCAGA 
CACCCCGGAG ACCCCGAGAG GCTGCCCAAG GAAGTGTTGC TGAAGCGGGC GGCCGACTTG 
GCAGAGGCCC TGTACGGAGT GCCCAGCGGC AACCAGGAGC TCCTCCTGAA GCGCGCGGCG 
GACGTGGCCG AGGCTCTGTA CAGCGCCCCC CGCGCGCCGG CGCCGCTAGG ACCCCTGGCG 
CCCAGCCACC CGCACCCTGC CGTCGTGGGC ATCAACGCCT TCAGCAGCCC GCTGGCCATC 
GCCGTCGGGG ACGCCACGCC GGGGCCGGAG CCAGGGTATG CGCGCAGCTG TGGCAGCGCG 
TCCCCGCGCG GGTTCGCGCC CAGCCCCGGC TCACAGCAGA GCGGCTACGG CGGCAGCCTC 
GGAGCTGGCC TCGGCGGCTA CGGGGCCCCG GGCGTGACCG GCCTCGGTGT GCCCGGGTCC 
CCCAGCTTCC TCAATGGCTC CACGGCCACC TCACCCTTCG CCATCATGCC CTCTAGCCCC 
CCGCTGGCCT CTGCCTCCTC CATGTCCCTC CCGGCGGCTG CCCCTACCAC CAGCGTCTTC 
TCCTTCTCGC CTGTCCACAT GATCTCCGCT GTCAAACAGA GGAGCGCTTT TGCCCCCGTG 
CTGCGCCCGC CCAGCTCCCC ACCCCAGGCC TGCCCCAGAA CCCACGGAGA GGGGCTTCCA 
GACCAGCCTT TTGAAGATTC TGACAAGTTT CACTCTCCAG CTCGGGGACT TCAAGGCCTG 
GCATACTCCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed