TTC3 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_005202058.3	XP_005202115.1	E3 ubiquitin-protein ligase TTC3 isoform X1
XM_010801674.1	XP_010799976.1	E3 ubiquitin-protein ligase TTC3 isoform X2
XM_024999925.1	XP_024855693.1	E3 ubiquitin-protein ligase TTC3 isoform X9
XM_005202057.3	XP_005202114.1	E3 ubiquitin-protein ligase TTC3 isoform X1
XM_010801677.2	XP_010799979.1	E3 ubiquitin-protein ligase TTC3 isoform X4
XM_024999711.1	XP_024855479.1	E3 ubiquitin-protein ligase TTC3 isoform X1
XM_005202057.4	XP_005202114.1	E3 ubiquitin-protein ligase TTC3 isoform X2
XM_010801675.2	XP_010799977.2	E3 ubiquitin-protein ligase TTC3 isoform X4
XM_024999841.1	XP_024855609.1	E3 ubiquitin-protein ligase TTC3 isoform X6
XM_005202060.2	XP_005202117.1	E3 ubiquitin-protein ligase TTC3 isoform X10
XM_010801675.1	XP_010799977.1	E3 ubiquitin-protein ligase TTC3 isoform X3
XM_005202061.3	XP_005202118.1	E3 ubiquitin-protein ligase TTC3 isoform X7
XM_025000044.1	XP_024855812.1	E3 ubiquitin-protein ligase TTC3 isoform X11
NM_001109767.3	NP_001103237.2	E3 ubiquitin-protein ligase TTC3
XM_005202056.3	XP_005202113.1	E3 ubiquitin-protein ligase TTC3 isoform X1
XM_010801679.2	XP_010799981.1	E3 ubiquitin-protein ligase TTC3 isoform X6
XM_005202060.1	XP_005202117.1	E3 ubiquitin-protein ligase TTC3 isoform X7
XM_024999804.1	XP_024855572.1	E3 ubiquitin-protein ligase TTC3 isoform X5
XM_005202061.4	XP_005202118.1	E3 ubiquitin-protein ligase TTC3 isoform X10
XM_010801678.2	XP_010799980.1	E3 ubiquitin-protein ligase TTC3 isoform X5
XM_005202056.4	XP_005202113.1	E3 ubiquitin-protein ligase TTC3 isoform X2
XM_005202058.4	XP_005202115.1	E3 ubiquitin-protein ligase TTC3 isoform X2
XM_010801674.2	XP_010799976.2	E3 ubiquitin-protein ligase TTC3 isoform X3
XM_010801678.3	XP_010799980.2	E3 ubiquitin-protein ligase TTC3 isoform X7
XM_010801679.3	XP_010799981.1	E3 ubiquitin-protein ligase TTC3 isoform X8
XM_024999992.1	XP_024855760.1	E3 ubiquitin-protein ligase TTC3 isoform X10

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa218662	XM_025000044.1 Latest version!	Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa218653	XM_005202057.4 Latest version!	Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa218653	XM_005202058.4 Latest version!	Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa218653	XM_005202056.4 Latest version!	Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa218654	XM_010801674.2 Latest version!	Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa218655	XM_010801675.2 Latest version!	Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa218656	XM_024999804.1 Latest version!	Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa218657	XM_024999841.1 Latest version!	Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa218658	XM_010801678.3 Latest version!	Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa218659	XM_010801679.3 Latest version!	Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa218660	XM_024999925.1 Latest version!	Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa218661	XM_005202061.4 Latest version!	Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa218661	XM_005202060.2 Latest version!	Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa218661	XM_024999992.1 Latest version!	Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa16127	NM_001109767.3 Latest version!	Bos taurus tetratricopeptide repeat domain 3 (TTC3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa16127	XM_005202057.3 Latest version!	Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa16127	XM_005202056.3 Latest version!	Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa16127	XM_005202058.3 Latest version!	Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa16129	XM_005202060.1 Latest version!	Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa16129	XM_005202061.3 Latest version!	Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa46601	XM_010801674.1 Latest version!	Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa46602	XM_010801675.1 Latest version!	Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa46604	XM_010801677.2 Latest version!	Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa46605	XM_010801678.2 Latest version!	Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa46606	XM_010801679.2 Latest version!	Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa218652	XM_024999711.1 Latest version!	Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

TTC3 ( XM_025000044.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855812.1 Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OBa218662
Clone ID Related Accession (Same CDS sequence)	XM_025000044.1
Accession Version	XM_025000044.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TTC3 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATAAAAT TGACAAGGAT TTTCTGCAAG GAATCTGTCT TACTCCTGAT TGTGAAGGAA 
TCATTTCAAA GATTATCATC TTCAGCAGTG GTGGTCAAGT TAAATGTGAA CCTAGAGAAG 
TTGAGGCTGA AAGAAGACAA AAAATTGAAG CGAAAGCTCC AAAAAAAGGA AGCAAAAAAA 
TTAGCACAAG AAAGACTGGA AGAGGACTTA AGGGAAAGTA ACCCACCAAA AACTGAAGAG 
CAGAGAGAAA CTATCAACCA TGTCCAAAGT TGCCAGTTCC TTGATGACAG AATTCTGCAG 
TGCATAAAGC AGTACGCTGA CAAGATTAAG TCCGGGATAC TGAATACTTC CAAGCTTCTC 
AAAGAATTGC TTTCTTGGAA GGTTTTAACC ACAGAAGACT ACACAACGTG TTTTTCTAGC 
AGAAATTTCC TGAATGAAGC AGTGGACTAT GTCATTCGTC ATTTGATCCA AGAAAAGAAT 
AGAGTAAAGA CAAGGGTATT TCTGCATGTT TTGAGTGAGC TTAAAGAAGT GGACCCAAAA 
CTAGCCACCT GGATCCAGAA ACTGAATAGC TTTGGCTTAG ATGCCACAGG ACCTTTCTTT 
TCTCGTTATG GGGCTCCTCT TAAGGAGCTT GATTTTAGCA TCATGACTTT TCTCTGGAAT 
GAGAAATACG GTCATAAGCT AGGCTCTATA GAAGGAAAGC AACTTGATTA TTTCTATGAG 
CCTACATCAC TGAAGGAAGC GCGGTGTTTA ATATGGCTGC TAGAAGAACA CAGAGACAAG 
TTCCCAGCAC TGCATAATGC CTTGGACGAG TTCTTTGATA TAATGGACAG CCGCTGCACT 
GTATTAAGGA AACAGGACAG TGGTGACGTG CCATTTAATT CTACCAAGAT AAAAAACAAA 
GGCAAGAAAA AGAAGCCAAA AGATTCAAAG CCTATGTTAG TTGGATCTGG AACACCTTCA 
GTCACACCAA ACAATGAGAC TGTCACTTCA GGTGAAGACC ATATCAAACG CAGTTTAAGT 
TCTGCGGGCC CCTTTGTAGT GCCTGATCAC CTTCGGCAGG ATATAGAAGA ATTTGAAGCT 
CTGTATGAAC AGCATAGTAA TGAGTATGTT GTCCGTAATA AGAAGCTGTG GGACATAAAC 
CCAAAACAGA AATGTTCAAC TCTATACGAT TATTTCTCTC AGTTGTTGGA GGAACATGGT 
CCCTTGGACA TGAGTAACAA AATGTTTTCT GAAGAATATG AGTTCTTCCC AGAAGAAACT 
CAACAGATAC TAGAAAAAGC AGGAGGCTTG AAAGCGTTTC TCCTGGGATG TCCTCGTTTT 
GTTGTGATTG ATAACTGTAT CGCATTGAAG AAAGTTGCAT TACGGCTCAA GAAAAAGAGA 
AAGAAGAAAA ACATTAAAAC AAAAGTAGAA GAAATTTCAA AAACAGGAGA GTATTTACGA 
GTTAAACTAC CACTGAATCC AACTGCTAGG GAATTTAAAC CAGATGTAAA GTCCAAACCA 
GTGTCTGACT TACCTTCAGC ACCAGCTTCT GAGGACATGA AGCCTCAGCC TGCACCTGCT 
AATTCTCCCC AATCAGCTCG TGACACTGGG AAGCCAAAAC CAGTTTTCGA TAATTCTTCC 
AGATCAGTTT CTGAGGATGA GAAGCCCAAA GGGATGTCTT CTGATTCTCC CAGGCCAGTC 
TTTGAGGATA CCGGTCACAA GCCGGTCTCC TCTAATTCTC CCAAACCAGT TCCTGAGGAA 
GTGAAAGCGA CCTATTGGCC TCGACCCCAG TTGGTCACAG GATACTGTAC ATATCTTCCC 
TTTCGGGGAT TTGACATTAC CCAGACTCCA CCAGCCTTCA TCAGTGTGTT ACCGACTTTG 
CCCCAGTATA GCATTTACAC CCCTCTGGCC AGTGTTCCTA CTGAGTATCA CCTGCAAAGA 
CCAATACCAG TGGTGCCGTC TTTTGTAGCC AGTGACATAA CGGAAGTGCA TTTTGAGGCT 
CATCATTTCA GTGCTGAGAA TGCCCCTGGT ACCCAGATTG CCTCCGAAAC ACAGATCCTT 
GAGGAATCTT TGGAAACACC AGTGAAGTCA GAGTGCAGCT ATGATGGTGC TGATATAGTC 
CAGAGTGAGG CTAGCAGAAA CGATGACTAC TGTGGCGATT CTGCCAAATG GGAAGTAAAT 
CCAGAAAGCA CCAATGAAGT AACAAATATT CCATGTACAC AAGTGGTTGC TGTACAGGTG 
TCTTGGAATA TAACACATCA GGAAGTCAAT ACTGAGCCAT ATGGTCCTTT TGAGGCACAA 
CAGGGGGGTA TTTCACAGGT TGAAAAGGAG TACCAGGTTT TACAAGAGCA GCTTAAGGAA 
GCATGTGAAA ATTATGAACA CAGCAATGTC AAGGGCTCAG AAGAAACCAA GGACTTGGAA 
GAAAAGTTGA AAAGGAATGT TGAAGAAAAT AAGATCTCAA AGACAGAACT AGATTGGTTC 
CTTGAAGATT TGAACAAGGA AATTAAAAAA TGGGAACAGG AGAAAAAAGA AATCCAAGAA 
AGACTAAAGG CACTGAAGAA GAAAATGAAA AAGGTGTTAA ATGCCAGCAA AATGTATACG 
CAGGAAAATG ATGGAAAGGA AAAGGCACTT GAATTACTTC TGGATCAGTC CCTTGAAATC 
AGTAATACAT TTACAAATGA GAAAATGAAA ATAGAAGAGA GTATAAAGAA AGGGAAAGAG 
CATTATGAAG AGAGTCTTCA AAGGGCTGTG GATGCAGAGG TATCTGTGCT TGAAAACTGG 
AAGGACATGG AAGTGTGTAA GTTACAGAGC ATGGAGTCAC AAGCCGAAAG GTTTCTCAAG 
AATCTGAAGC TAATGAGCAG TGATTCGGCA GCGTATTCTG ACATGGACTC AGACATACAT 
TCATGGGAAT CGTTTCTTTC TGATGTTAGA GAAGAAATTG AGAAAGCAAA GTCTCAGTTT 
GAAGAACAAA TTCAGGCAAT TAAATATGGT TCTCAGCTCA GTGAACTTCC TAAAGTGCAG 
ATTTCTGAGC TTTCATTTCC TACCTGTAAC ACGATTCATC CTAAATTACT TCCTGAGTCT 
TCAGGCCAGG ATGGTCAAGG TCCCTCCACT TCTACAAGTC ATGGGACCGG AGACCAGACA 
GCAGCTCCCG AGGAGTCAAG TCTGGGCTCT GCAGCTGACA GCCCAGTGGA GTTGCCCTCC 
TGCAGTCAGA AAGCCCCCCA GCTGCCAGAG CATGAGGGTG CTGGCCAGGC AACTGGGCCA 
GAACCAGGCC CAGTGGCTGA TCAGAAGTTA CCTGCTGCTC CAGAGCGTGC CACTCATTCA 
AGCCAGTCTC CAAAGAAGCC GTTCAATAGC ATTATTGAGC ACCTGTTGGT GGTGTTCCCG 
TGTTACAGCA GCACGGAGCT TGCTGGTTTT ATTAAAAAAG TTAGAAACAA AAACAAGACC 
TCTCTGTCTG GATTGAGTAT TGACGAAATT GTGCAAAGAG TGACAGAACA CGTCCTTGAT 
GAAGAAAGAA AGAAAAAGCC AAGTCCAGGA AAGGGCTGGA GGCTGTCTGC ACCCAGCTCT 
GCTGTTGCTG TGCCCAGGGC TGCCCAGGAC CCGCCCTCCA TGCCTGTCGG AGCATCGCCC 
GAAACCAGGG GGCCGAGGAC CGAGGACGTC CCTGTGTCCT GCGCAAATTC CTGTGAAATG 
TGCCGAGAAG TATTCAAGTC CAAACACGTG CGTGAGCTGC AGTGTGGGCA CAGGCTTCAC 
AAGGGGTGCT TCAAGCAGTG GCTGAAGAGC CAGCGCACCT GCCCAGCCTG CCTGGGTCAC 
GACCTCCTGT CAGAGGAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855812.1
CDS	521..4321
Translation	MIKLTRIFCKESVLLLIVKESFQRLSSSAVVVKLNVNLEKLRLKEDKKLKRKLQKKEAKKLAQERLEEDLRESNPPKTEEQRETINHVQSCQFLDDRILQCIKQYADKIKSGILNTSKLLKELLSWKVLTTEDYTTCFSSRNFLNEAVDYVIRHLIQEKNRVKTRVFLHVLSELKEVDPKLATWIQKLNSFGLDATGPFFSRYGAPLKELDFSIMTFLWNEKYGHKLGSIEGKQLDYFYEPTSLKEARCLIWLLEEHRDKFPALHNALDEFFDIMDSRCTVLRKQDSGDVPFNSTKIKNKGKKKKPKDSKPMLVGSGTPSVTPNNETVTSGEDHIKRSLSSAGPFVVPDHLRQDIEEFEALYEQHSNEYVVRNKKLWDINPKQKCSTLYDYFSQLLEEHGPLDMSNKMFSEEYEFFPEETQQILEKAGGLKAFLLGCPRFVVIDNCIALKKVALRLKKKRKKKNIKTKVEEISKTGEYLRVKLPLNPTAREFKPDVKSKPVSDLPSAPASEDMKPQPAPANSPQSARDTGKPKPVFDNSSRSVSEDEKPKGMSSDSPRPVFEDTGHKPVSSNSPKPVPEEVKATYWPRPQLVTGYCTYLPFRGFDITQTPPAFISVLPTLPQYSIYTPLASVPTEYHLQRPIPVVPSFVASDITEVHFEAHHFSAENAPGTQIASETQILEESLETPVKSECSYDGADIVQSEASRNDDYCGDSAKWEVNPESTNEVTNIPCTQVVAVQVSWNITHQEVNTEPYGPFEAQQGGISQVEKEYQVLQEQLKEACENYEHSNVKGSEETKDLEEKLKRNVEENKISKTELDWFLEDLNKEIKKWEQEKKEIQERLKALKKKMKKVLNASKMYTQENDGKEKALELLLDQSLEISNTFTNEKMKIEESIKKGKEHYEESLQRAVDAEVSVLENWKDMEVCKLQSMESQAERFLKNLKLMSSDSAAYSDMDSDIHSWESFLSDVREEIEKAKSQFEEQIQAIKYGSQLSELPKVQISELSFPTCNTIHPKLLPESSGQDGQGPSTSTSHGTGDQTAAPEESSLGSAADSPVELPSCSQKAPQLPEHEGAGQATGPEPGPVADQKLPAAPERATHSSQSPKKPFNSIIEHLLVVFPCYSSTELAGFIKKVRNKNKTSLSGLSIDEIVQRVTEHVLDEERKKKPSPGKGWRLSAPSSAVAVPRAAQDPPSMPVGASPETRGPRTEDVPVSCANSCEMCREVFKSKHVRELQCGHRLHKGCFKQWLKSQRTCPACLGHDLLSEE

Target ORF information:

RefSeq Version	XM_025000044.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025000044.1

ORF Insert Sequence:

ATGATAAAAT TGACAAGGAT TTTCTGCAAG GAATCTGTCT TACTCCTGAT TGTGAAGGAA 
TCATTTCAAA GATTATCATC TTCAGCAGTG GTGGTCAAGT TAAATGTGAA CCTAGAGAAG 
TTGAGGCTGA AAGAAGACAA AAAATTGAAG CGAAAGCTCC AAAAAAAGGA AGCAAAAAAA 
TTAGCACAAG AAAGACTGGA AGAGGACTTA AGGGAAAGTA ACCCACCAAA AACTGAAGAG 
CAGAGAGAAA CTATCAACCA TGTCCAAAGT TGCCAGTTCC TTGATGACAG AATTCTGCAG 
TGCATAAAGC AGTACGCTGA CAAGATTAAG TCCGGGATAC TGAATACTTC CAAGCTTCTC 
AAAGAATTGC TTTCTTGGAA GGTTTTAACC ACAGAAGACT ACACAACGTG TTTTTCTAGC 
AGAAATTTCC TGAATGAAGC AGTGGACTAT GTCATTCGTC ATTTGATCCA AGAAAAGAAT 
AGAGTAAAGA CAAGGGTATT TCTGCATGTT TTGAGTGAGC TTAAAGAAGT GGACCCAAAA 
CTAGCCACCT GGATCCAGAA ACTGAATAGC TTTGGCTTAG ATGCCACAGG ACCTTTCTTT 
TCTCGTTATG GGGCTCCTCT TAAGGAGCTT GATTTTAGCA TCATGACTTT TCTCTGGAAT 
GAGAAATACG GTCATAAGCT AGGCTCTATA GAAGGAAAGC AACTTGATTA TTTCTATGAG 
CCTACATCAC TGAAGGAAGC GCGGTGTTTA ATATGGCTGC TAGAAGAACA CAGAGACAAG 
TTCCCAGCAC TGCATAATGC CTTGGACGAG TTCTTTGATA TAATGGACAG CCGCTGCACT 
GTATTAAGGA AACAGGACAG TGGTGACGTG CCATTTAATT CTACCAAGAT AAAAAACAAA 
GGCAAGAAAA AGAAGCCAAA AGATTCAAAG CCTATGTTAG TTGGATCTGG AACACCTTCA 
GTCACACCAA ACAATGAGAC TGTCACTTCA GGTGAAGACC ATATCAAACG CAGTTTAAGT 
TCTGCGGGCC CCTTTGTAGT GCCTGATCAC CTTCGGCAGG ATATAGAAGA ATTTGAAGCT 
CTGTATGAAC AGCATAGTAA TGAGTATGTT GTCCGTAATA AGAAGCTGTG GGACATAAAC 
CCAAAACAGA AATGTTCAAC TCTATACGAT TATTTCTCTC AGTTGTTGGA GGAACATGGT 
CCCTTGGACA TGAGTAACAA AATGTTTTCT GAAGAATATG AGTTCTTCCC AGAAGAAACT 
CAACAGATAC TAGAAAAAGC AGGAGGCTTG AAAGCGTTTC TCCTGGGATG TCCTCGTTTT 
GTTGTGATTG ATAACTGTAT CGCATTGAAG AAAGTTGCAT TACGGCTCAA GAAAAAGAGA 
AAGAAGAAAA ACATTAAAAC AAAAGTAGAA GAAATTTCAA AAACAGGAGA GTATTTACGA 
GTTAAACTAC CACTGAATCC AACTGCTAGG GAATTTAAAC CAGATGTAAA GTCCAAACCA 
GTGTCTGACT TACCTTCAGC ACCAGCTTCT GAGGACATGA AGCCTCAGCC TGCACCTGCT 
AATTCTCCCC AATCAGCTCG TGACACTGGG AAGCCAAAAC CAGTTTTCGA TAATTCTTCC 
AGATCAGTTT CTGAGGATGA GAAGCCCAAA GGGATGTCTT CTGATTCTCC CAGGCCAGTC 
TTTGAGGATA CCGGTCACAA GCCGGTCTCC TCTAATTCTC CCAAACCAGT TCCTGAGGAA 
GTGAAAGCGA CCTATTGGCC TCGACCCCAG TTGGTCACAG GATACTGTAC ATATCTTCCC 
TTTCGGGGAT TTGACATTAC CCAGACTCCA CCAGCCTTCA TCAGTGTGTT ACCGACTTTG 
CCCCAGTATA GCATTTACAC CCCTCTGGCC AGTGTTCCTA CTGAGTATCA CCTGCAAAGA 
CCAATACCAG TGGTGCCGTC TTTTGTAGCC AGTGACATAA CGGAAGTGCA TTTTGAGGCT 
CATCATTTCA GTGCTGAGAA TGCCCCTGGT ACCCAGATTG CCTCCGAAAC ACAGATCCTT 
GAGGAATCTT TGGAAACACC AGTGAAGTCA GAGTGCAGCT ATGATGGTGC TGATATAGTC 
CAGAGTGAGG CTAGCAGAAA CGATGACTAC TGTGGCGATT CTGCCAAATG GGAAGTAAAT 
CCAGAAAGCA CCAATGAAGT AACAAATATT CCATGTACAC AAGTGGTTGC TGTACAGGTG 
TCTTGGAATA TAACACATCA GGAAGTCAAT ACTGAGCCAT ATGGTCCTTT TGAGGCACAA 
CAGGGGGGTA TTTCACAGGT TGAAAAGGAG TACCAGGTTT TACAAGAGCA GCTTAAGGAA 
GCATGTGAAA ATTATGAACA CAGCAATGTC AAGGGCTCAG AAGAAACCAA GGACTTGGAA 
GAAAAGTTGA AAAGGAATGT TGAAGAAAAT AAGATCTCAA AGACAGAACT AGATTGGTTC 
CTTGAAGATT TGAACAAGGA AATTAAAAAA TGGGAACAGG AGAAAAAAGA AATCCAAGAA 
AGACTAAAGG CACTGAAGAA GAAAATGAAA AAGGTGTTAA ATGCCAGCAA AATGTATACG 
CAGGAAAATG ATGGAAAGGA AAAGGCACTT GAATTACTTC TGGATCAGTC CCTTGAAATC 
AGTAATACAT TTACAAATGA GAAAATGAAA ATAGAAGAGA GTATAAAGAA AGGGAAAGAG 
CATTATGAAG AGAGTCTTCA AAGGGCTGTG GATGCAGAGG TATCTGTGCT TGAAAACTGG 
AAGGACATGG AAGTGTGTAA GTTACAGAGC ATGGAGTCAC AAGCCGAAAG GTTTCTCAAG 
AATCTGAAGC TAATGAGCAG TGATTCGGCA GCGTATTCTG ACATGGACTC AGACATACAT 
TCATGGGAAT CGTTTCTTTC TGATGTTAGA GAAGAAATTG AGAAAGCAAA GTCTCAGTTT 
GAAGAACAAA TTCAGGCAAT TAAATATGGT TCTCAGCTCA GTGAACTTCC TAAAGTGCAG 
ATTTCTGAGC TTTCATTTCC TACCTGTAAC ACGATTCATC CTAAATTACT TCCTGAGTCT 
TCAGGCCAGG ATGGTCAAGG TCCCTCCACT TCTACAAGTC ATGGGACCGG AGACCAGACA 
GCAGCTCCCG AGGAGTCAAG TCTGGGCTCT GCAGCTGACA GCCCAGTGGA GTTGCCCTCC 
TGCAGTCAGA AAGCCCCCCA GCTGCCAGAG CATGAGGGTG CTGGCCAGGC AACTGGGCCA 
GAACCAGGCC CAGTGGCTGA TCAGAAGTTA CCTGCTGCTC CAGAGCGTGC CACTCATTCA 
AGCCAGTCTC CAAAGAAGCC GTTCAATAGC ATTATTGAGC ACCTGTTGGT GGTGTTCCCG 
TGTTACAGCA GCACGGAGCT TGCTGGTTTT ATTAAAAAAG TTAGAAACAA AAACAAGACC 
TCTCTGTCTG GATTGAGTAT TGACGAAATT GTGCAAAGAG TGACAGAACA CGTCCTTGAT 
GAAGAAAGAA AGAAAAAGCC AAGTCCAGGA AAGGGCTGGA GGCTGTCTGC ACCCAGCTCT 
GCTGTTGCTG TGCCCAGGGC TGCCCAGGAC CCGCCCTCCA TGCCTGTCGG AGCATCGCCC 
GAAACCAGGG GGCCGAGGAC CGAGGACGTC CCTGTGTCCT GCGCAAATTC CTGTGAAATG 
TGCCGAGAAG TATTCAAGTC CAAACACGTG CGTGAGCTGC AGTGTGGGCA CAGGCTTCAC 
AAGGGGTGCT TCAAGCAGTG GCTGAAGAGC CAGCGCACCT GCCCAGCCTG CCTGGGTCAC 
GACCTCCTGT CAGAGGAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_005202057.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005202114.1 Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa218653
Clone ID Related Accession (Same CDS sequence)	XM_005202057.4 , XM_005202056.4 , XM_005202058.4
Accession Version	XM_005202057.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5958bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TTC3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005202057.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGATT TTCCCCAGGG AAATTTTGCA GATTATGCCT TGTTAGAAGA TTGCCCTTAT 
GTGGATGATT GTATCTTTGC CCCTGAGTTT ATGTCCAGTG ATTATGTTCG TGTGACTCAG 
CTTTACTGTG ATGGAGTGGG TAGACAGTAT AAAGATTATG CCCAAACTGA GGGAAACTTA 
GAATTTGACA TCTGCACTCT GTGGTGTAGT CAACCTATTT CTGTCCTGCA AGATTACTGT 
GATGCCATTA AAGTCCACAT CTTCTGGCCA CTTCTGTTTC AACGTCAGCA GAGCTCTATA 
ATAACACGGA TGCACTCCTG TGTAAGGACC AGCGGTTCTC ATGCTTGTGA GATAAGTTTG 
AAGAAATTAC AGCAGCTTGA ACTGATGGAA GATATTGTGG ATTTGGCAAA GAAAGTTGTG 
AATGATACAT TCTTTATCGG AGGCTTATTG AGAATTGGCT ATAAAATAGA GAATAAAATC 
TTGGCAATGG AAGAAGCTTT GAATTGGGTG AAATACACAG GCGATGTAAC AATTTTATCT 
AAATTAGGAG CAGTTGATAA TTGTTGGCGT ATGTTAAGTA TTTTCTTTAC TGAATACAAG 
TATCACATAA CTAAAGTTGT AACAGAAAAC TGCAGTTTGC TTGAAGAATT TAAAACTCAA 
AGTTGTGCGG AGTGTCTAGA GCAAGGAGAA CTAATGAAAA TGAAAGGAAA TGAAGAGTTT 
TCCAAAGAAA GATATGATAT AGCTATTATC TATTACACCA GAGCCATTGA ATGTAGGCCT 
GAAAATCACC TGCTTTATGG TAACCGAGCT CTTTGTTTTC TTCGTACTGG ACAATTCAGA 
AATGCACTTG GTGATGGAAA GAGAGCTACC ATTCTGAAGA ACAATTGGAC AAAGGGTCAT 
TATCGTTATT GTGCTGCTCT TTCTATGCTG GGGGAATATG ACTGGGCCCT GCAAGCAAAC 
ATAAAAGCTC AAAAACTCTG TAAAAATGAC CCTGAGGGAA TCAAGGATCT AATTCAGCAG 
CATGTAAAGT TACAAAAACA AATAGAAGAC CTGCAAGGTC AAACACCAGC TAGTAATCCA 
GTCAAAGCCT TCTGTGAAAG CAGGGCCTAC ATACCTAGTT TATCAGCACC TGCATTTAGT 
ACTTCACTTA ACTTTGTGGA AACTGAAAAA AATTTCAGAA AAACTAACCA CGAAATGGCA 
AACGGTGGTA ATCAGAATCC AAAGGTGGTA GATGAAGCTC TGAAAGTAGA TGATTGTGAC 
TGTCATCCTG AATTTGTACC GCCATCAAGT CAGCCTCCAA AATATAAAGG GAAACAAAAA 
TCTCGAAACA ATGAATTGGA GAAGTTCAGC CCTGGTTCAC AAGAAACTTT ACCAGTAGAT 
TTAAAAAACA TCTTGGAGAA ACAGTTTTCT AAATCTTCCA GAGCCGCACA CCAGGATTTT 
GCTAATATAA TGAAAATGTT GAGAAGTTTA ATTCAGGATG GCTACACGGC CTTGCTGGAG 
CAGCGCTGCC GCAGCGCCGC CCAAGCCTTC ACAGAGTTGC TCAACGGTTT AGATCCTCAA 
AAAATAAAGC AATTAAACCT GGCCATGATT AACTACGTGT TAGTAGTCTA CGGACTTGCC 
ATTTCACTCC TTGGAATAGG ACAGCCCGAG GAGTTATCTG AAGCTGAAAA CCAGTTCAAG 
AGGATGATTG AACACTACCC CAATGAGGGG CTTGATTGCT TGGCCTACTG TGGAATTGGA 
AAAGTGTATT TGAAAAAAAA CAGATTTTTA GAAGCTCTTA ATCATTTTGA GAAAGCAAGA 
ACCTTGATCT GTCGTCTTCC TGGAGTGTTA ACTTGGCCCA CAAGTAATGT GGTTATTGAG 
GAGACTCAGC CAGGGAAGAT AAAGATGCTG TTAGAGAAAT TTGTTGAAGA ATGCAGGTTC 
CCTCCAGTGC CGGATGCCAC TTGTTGCTAT CAGAAGTGCC ATGGATATTC CAAAATCCAG 
ATATATATAA CTGATCCAGA CTTTAAGGGT TTTATACGGA TCAGCTGTTG CCAGTATTGT 
AAAATAGAAT TTCACATGAA CTGCTGGAAG AAGTTAAAAA CAACAACCTT CAATGATAAA 
ATTGACAAGG ATTTTCTGCA AGGAATCTGT CTTACTCCTG ATTGTGAAGG AATCATTTCA 
AAGATTATCA TCTTCAGCAG TGGTGGTCAA GTTAAATGTG AATTTGAACA CAAGGTCATT 
AAAGAAAAGG TTCCTCCAAG ACCTATTCTG AAACAGAAAT GTTCTAGCCT AGAGAAGTTG 
AGGCTGAAAG AAGACAAAAA ATTGAAGCGA AAGCTCCAAA AAAAGGAAGC AAAAAAATTA 
GCACAAGAAA GACTGGAAGA GGACTTAAGG GAAAGTAACC CACCAAAAAC TGAAGAGCAG 
AGAGAAACTA TCAACCATGT CCAAAGTTGC CAGTTCCTTG ATGACAGAAT TCTGCAGTGC 
ATAAAGCAGT ACGCTGACAA GATTAAGTCC GGGATACTGA ATACTTCCAA GCTTCTCAAA 
GAATTGCTTT CTTGGAAGGT TTTAACCACA GAAGACTACA CAACGTGTTT TTCTAGCAGA 
AATTTCCTGA ATGAAGCAGT GGACTATGTC ATTCGTCATT TGATCCAAGA AAAGAATAGA 
GTAAAGACAA GGGTATTTCT GCATGTTTTG AGTGAGCTTA AAGAAGTGGA CCCAAAACTA 
GCCACCTGGA TCCAGAAACT GAATAGCTTT GGCTTAGATG CCACAGGACC TTTCTTTTCT 
CGTTATGGGG CTCCTCTTAA GGAGCTTGAT TTTAGCATCA TGACTTTTCT CTGGAATGAG 
AAATACGGTC ATAAGCTAGG CTCTATAGAA GGAAAGCAAC TTGATTATTT CTATGAGCCT 
ACATCACTGA AGGAAGCGCG GTGTTTAATA TGGCTGCTAG AAGAACACAG AGACAAGTTC 
CCAGCACTGC ATAATGCCTT GGACGAGTTC TTTGATATAA TGGACAGCCG CTGCACTGTA 
TTAAGGAAAC AGGACAGTGG TGACGTGCCA TTTAATTCTA CCAAGATAAA AAACAAAGGC 
AAGAAAAAGA AGCCAAAAGA TTCAAAGCCT ATGTTAGTTG GATCTGGAAC ACCTTCAGTC 
ACACCAAACA ATGAGACTGT CACTTCAGGT GAAGACCATA TCAAACGCAG TTTAAGTTCT 
GCGGGCCCCT TTGTAGTGCC TGATCACCTT CGGCAGGATA TAGAAGAATT TGAAGCTCTG 
TATGAACAGC ATAGTAATGA GTATGTTGTC CGTAATAAGA AGCTGTGGGA CATAAACCCA 
AAACAGAAAT GTTCAACTCT ATACGATTAT TTCTCTCAGT TGTTGGAGGA ACATGGTCCC 
TTGGACATGA GTAACAAAAT GTTTTCTGAA GAATATGAGT TCTTCCCAGA AGAAACTCAA 
CAGATACTAG AAAAAGCAGG AGGCTTGAAA GCGTTTCTCC TGGGATGTCC TCGTTTTGTT 
GTGATTGATA ACTGTATCGC ATTGAAGAAA GTTGCATTAC GGCTCAAGAA AAAGAGAAAG 
AAGAAAAACA TTAAAACAAA AGTAGAAGAA ATTTCAAAAA CAGGAGAGTA TTTACGAGTT 
AAACTACCAC TGAATCCAAC TGCTAGGGAA TTTAAACCAG ATGTAAAGTC CAAACCAGTG 
TCTGACTTAC CTTCAGCACC AGCTTCTGAG GACATGAAGC CTCAGCCTGC ACCTGCTAAT 
TCTCCCCAAT CAGCTCGTGA CACTGGGAAG CCAAAACCAG TTTTCGATAA TTCTTCCAGA 
TCAGTTTCTG AGGATGAGAA GCCCAAAGGG ATGTCTTCTG ATTCTCCCAG GCCAGTCTTT 
GAGGATACCG GTCACAAGCC GGTCTCCTCT AATTCTCCCA AACCAGTTCC TGAGGAAGTG 
AAAGCGACCT ATTGGCCTCG ACCCCAGTTG GTCACAGGAT ACTGTACATA TCTTCCCTTT 
CGGGGATTTG ACATTACCCA GACTCCACCA GCCTTCATCA GTGTGTTACC GACTTTGCCC 
CAGTATAGCA TTTACACCCC TCTGGCCAGT GTTCCTACTG AGTATCACCT GCAAAGACCA 
ATACCAGTGG TGCCGTCTTT TGTAGCCAGT GACATAACGG AAGTGCATTT TGAGGCTCAT 
CATTTCAGTG CTGAGAATGC CCCTGGTACC CAGATTGCCT CCGAAACACA GATCCTTGAG 
GAATCTTTGG AAACACCAGT GAAGTCAGAG TGCAGCTATG ATGGTGCTGA TATAGTCCAG 
AGTGAGGCTA GCAGAAACGA TGACTACTGT GGCGATTCTG CCAAATGGGA AGTAAATCCA 
GAAAGCACCA ATGAAGTAAC AAATATTCCA TGTACACAAG TGGTTGCTGT ACAGGTGTCT 
TGGAATATAA CACATCAGGA AGTCAATACT GAGCCATATG GTCCTTTTGA GGCACAACAG 
GGGGGTATTT CACAGGTTGA AAAGGAGTAC CAGGTTTTAC AAGAGCAGCT TAAGGAAGCA 
TGTGAAAATT ATGAACACAG CAATGTCAAG GGCTCAGAAG AAACCAAGGA CTTGGAAGAA 
AAGTTGAAAA GGAATGTTGA AGAAAATAAG ATCTCAAAGA CAGAACTAGA TTGGTTCCTT 
GAAGATTTGA ACAAGGAAAT TAAAAAATGG GAACAGGAGA AAAAAGAAAT CCAAGAAAGA 
CTAAAGGCAC TGAAGAAGAA AATGAAAAAG GTGTTAAATG CCAGCAAAAT GTATACGCAG 
GAAAATGATG GAAAGGAAAA GGCACTTGAA TTACTTCTGG ATCAGTCCCT TGAAATCAGT 
AATACATTTA CAAATGAGAA AATGAAAATA GAAGAGAGTA TAAAGAAAGG GAAAGAGCAT 
TATGAAGAGA GTCTTCAAAG GGCTGTGGAT GCAGAGGTAT CTGTGCTTGA AAACTGGAAG 
GACATGGAAG TGTGTAAGTT ACAGAGCATG GAGTCACAAG CCGAAAGGTT TCTCAAGAAT 
CTGAAGCTAA TGAGCAGTGA TTCGGCAGCG TATTCTGACA TGGACTCAGA CATACATTCA 
TGGGAATCGT TTCTTTCTGA TGTTAGAGAA GAAATTGAGA AAGCAAAGTC TCAGTTTGAA 
GAACAAATTC AGGCAATTAA ATATGGTTCT CAGCTCAGTG AACTTCCTAA AGTGCAGATT 
TCTGAGCTTT CATTTCCTAC CTGTAACACG ATTCATCCTA AATTACTTCC TGAGTCTTCA 
GGCCAGGATG GTCAAGGTCC CTCCACTTCT ACAAGTCATG GGACCGGAGA CCAGACAGCA 
GCTCCCGAGG AGTCAAGTCT GGGCTCTGCA GCTGACAGCC CAGTGGAGTT GCCCTCCTGC 
AGTCAGAAAG CCCCCCAGCT GCCAGAGCAT GAGGGTGCTG GCCAGGCAAC TGGGCCAGAA 
CCAGGCCCAG TGGCTGATCA GAAGTTACCT GCTGCTCCAG AGCGTGCCAC TCATTCAAGC 
CAGTCTCCAA AGAAGCCGTT CAATAGCATT ATTGAGCACC TGTTGGTGGT GTTCCCGTGT 
TACAGCAGCA CGGAGCTTGC TGGTTTTATT AAAAAAGTTA GAAACAAAAA CAAGACCTCT 
CTGTCTGGAT TGAGTATTGA CGAAATTGTG CAAAGAGTGA CAGAACACGT CCTTGATGAA 
GAAAGAAAGA AAAAGCCAAG TCCAGGAAAG GGCTGGAGGC TGTCTGCACC CAGCTCTGCT 
GTTGCTGTGC CCAGGGCTGC CCAGGACCCG CCCTCCATGC CTGTCGGAGC ATCGCCCGAA 
ACCAGGGGGC CGAGGACCGA GGACGTCCCT GTGTCCTGCG CAAATTCCTG TGAAATGTGC 
CGAGAAGTAT TCAAGTCCAA ACACGTGCGT GAGCTGCAGT GTGGGCACAG GCTTCACAAG 
GGGTGCTTCA AGCAGTGGCT GAAGAGCCAG CGCACCTGCC CAGCCTGCCT GGGTCACGAC 
CTCCTGTCAG AGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005202114.1
CDS	1978..7935
Translation	MADFPQGNFADYALLEDCPYVDDCIFAPEFMSSDYVRVTQLYCDGVGRQYKDYAQTEGNLEFDICTLWCSQPISVLQDYCDAIKVHIFWPLLFQRQQSSIITRMHSCVRTSGSHACEISLKKLQQLELMEDIVDLAKKVVNDTFFIGGLLRIGYKIENKILAMEEALNWVKYTGDVTILSKLGAVDNCWRMLSIFFTEYKYHITKVVTENCSLLEEFKTQSCAECLEQGELMKMKGNEEFSKERYDIAIIYYTRAIECRPENHLLYGNRALCFLRTGQFRNALGDGKRATILKNNWTKGHYRYCAALSMLGEYDWALQANIKAQKLCKNDPEGIKDLIQQHVKLQKQIEDLQGQTPASNPVKAFCESRAYIPSLSAPAFSTSLNFVETEKNFRKTNHEMANGGNQNPKVVDEALKVDDCDCHPEFVPPSSQPPKYKGKQKSRNNELEKFSPGSQETLPVDLKNILEKQFSKSSRAAHQDFANIMKMLRSLIQDGYTALLEQRCRSAAQAFTELLNGLDPQKIKQLNLAMINYVLVVYGLAISLLGIGQPEELSEAENQFKRMIEHYPNEGLDCLAYCGIGKVYLKKNRFLEALNHFEKARTLICRLPGVLTWPTSNVVIEETQPGKIKMLLEKFVEECRFPPVPDATCCYQKCHGYSKIQIYITDPDFKGFIRISCCQYCKIEFHMNCWKKLKTTTFNDKIDKDFLQGICLTPDCEGIISKIIIFSSGGQVKCEFEHKVIKEKVPPRPILKQKCSSLEKLRLKEDKKLKRKLQKKEAKKLAQERLEEDLRESNPPKTEEQRETINHVQSCQFLDDRILQCIKQYADKIKSGILNTSKLLKELLSWKVLTTEDYTTCFSSRNFLNEAVDYVIRHLIQEKNRVKTRVFLHVLSELKEVDPKLATWIQKLNSFGLDATGPFFSRYGAPLKELDFSIMTFLWNEKYGHKLGSIEGKQLDYFYEPTSLKEARCLIWLLEEHRDKFPALHNALDEFFDIMDSRCTVLRKQDSGDVPFNSTKIKNKGKKKKPKDSKPMLVGSGTPSVTPNNETVTSGEDHIKRSLSSAGPFVVPDHLRQDIEEFEALYEQHSNEYVVRNKKLWDINPKQKCSTLYDYFSQLLEEHGPLDMSNKMFSEEYEFFPEETQQILEKAGGLKAFLLGCPRFVVIDNCIALKKVALRLKKKRKKKNIKTKVEEISKTGEYLRVKLPLNPTAREFKPDVKSKPVSDLPSAPASEDMKPQPAPANSPQSARDTGKPKPVFDNSSRSVSEDEKPKGMSSDSPRPVFEDTGHKPVSSNSPKPVPEEVKATYWPRPQLVTGYCTYLPFRGFDITQTPPAFISVLPTLPQYSIYTPLASVPTEYHLQRPIPVVPSFVASDITEVHFEAHHFSAENAPGTQIASETQILEESLETPVKSECSYDGADIVQSEASRNDDYCGDSAKWEVNPESTNEVTNIPCTQVVAVQVSWNITHQEVNTEPYGPFEAQQGGISQVEKEYQVLQEQLKEACENYEHSNVKGSEETKDLEEKLKRNVEENKISKTELDWFLEDLNKEIKKWEQEKKEIQERLKALKKKMKKVLNASKMYTQENDGKEKALELLLDQSLEISNTFTNEKMKIEESIKKGKEHYEESLQRAVDAEVSVLENWKDMEVCKLQSMESQAERFLKNLKLMSSDSAAYSDMDSDIHSWESFLSDVREEIEKAKSQFEEQIQAIKYGSQLSELPKVQISELSFPTCNTIHPKLLPESSGQDGQGPSTSTSHGTGDQTAAPEESSLGSAADSPVELPSCSQKAPQLPEHEGAGQATGPEPGPVADQKLPAAPERATHSSQSPKKPFNSIIEHLLVVFPCYSSTELAGFIKKVRNKNKTSLSGLSIDEIVQRVTEHVLDEERKKKPSPGKGWRLSAPSSAVAVPRAAQDPPSMPVGASPETRGPRTEDVPVSCANSCEMCREVFKSKHVRELQCGHRLHKGCFKQWLKSQRTCPACLGHDLLSEE

Target ORF information:

RefSeq Version	XM_005202057.4
Organism	Bos taurus(cattle)
Definition	Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005202057.4

ORF Insert Sequence:

ATGGCCGATT TTCCCCAGGG AAATTTTGCA GATTATGCCT TGTTAGAAGA TTGCCCTTAT 
GTGGATGATT GTATCTTTGC CCCTGAGTTT ATGTCCAGTG ATTATGTTCG TGTGACTCAG 
CTTTACTGTG ATGGAGTGGG TAGACAGTAT AAAGATTATG CCCAAACTGA GGGAAACTTA 
GAATTTGACA TCTGCACTCT GTGGTGTAGT CAACCTATTT CTGTCCTGCA AGATTACTGT 
GATGCCATTA AAGTCCACAT CTTCTGGCCA CTTCTGTTTC AACGTCAGCA GAGCTCTATA 
ATAACACGGA TGCACTCCTG TGTAAGGACC AGCGGTTCTC ATGCTTGTGA GATAAGTTTG 
AAGAAATTAC AGCAGCTTGA ACTGATGGAA GATATTGTGG ATTTGGCAAA GAAAGTTGTG 
AATGATACAT TCTTTATCGG AGGCTTATTG AGAATTGGCT ATAAAATAGA GAATAAAATC 
TTGGCAATGG AAGAAGCTTT GAATTGGGTG AAATACACAG GCGATGTAAC AATTTTATCT 
AAATTAGGAG CAGTTGATAA TTGTTGGCGT ATGTTAAGTA TTTTCTTTAC TGAATACAAG 
TATCACATAA CTAAAGTTGT AACAGAAAAC TGCAGTTTGC TTGAAGAATT TAAAACTCAA 
AGTTGTGCGG AGTGTCTAGA GCAAGGAGAA CTAATGAAAA TGAAAGGAAA TGAAGAGTTT 
TCCAAAGAAA GATATGATAT AGCTATTATC TATTACACCA GAGCCATTGA ATGTAGGCCT 
GAAAATCACC TGCTTTATGG TAACCGAGCT CTTTGTTTTC TTCGTACTGG ACAATTCAGA 
AATGCACTTG GTGATGGAAA GAGAGCTACC ATTCTGAAGA ACAATTGGAC AAAGGGTCAT 
TATCGTTATT GTGCTGCTCT TTCTATGCTG GGGGAATATG ACTGGGCCCT GCAAGCAAAC 
ATAAAAGCTC AAAAACTCTG TAAAAATGAC CCTGAGGGAA TCAAGGATCT AATTCAGCAG 
CATGTAAAGT TACAAAAACA AATAGAAGAC CTGCAAGGTC AAACACCAGC TAGTAATCCA 
GTCAAAGCCT TCTGTGAAAG CAGGGCCTAC ATACCTAGTT TATCAGCACC TGCATTTAGT 
ACTTCACTTA ACTTTGTGGA AACTGAAAAA AATTTCAGAA AAACTAACCA CGAAATGGCA 
AACGGTGGTA ATCAGAATCC AAAGGTGGTA GATGAAGCTC TGAAAGTAGA TGATTGTGAC 
TGTCATCCTG AATTTGTACC GCCATCAAGT CAGCCTCCAA AATATAAAGG GAAACAAAAA 
TCTCGAAACA ATGAATTGGA GAAGTTCAGC CCTGGTTCAC AAGAAACTTT ACCAGTAGAT 
TTAAAAAACA TCTTGGAGAA ACAGTTTTCT AAATCTTCCA GAGCCGCACA CCAGGATTTT 
GCTAATATAA TGAAAATGTT GAGAAGTTTA ATTCAGGATG GCTACACGGC CTTGCTGGAG 
CAGCGCTGCC GCAGCGCCGC CCAAGCCTTC ACAGAGTTGC TCAACGGTTT AGATCCTCAA 
AAAATAAAGC AATTAAACCT GGCCATGATT AACTACGTGT TAGTAGTCTA CGGACTTGCC 
ATTTCACTCC TTGGAATAGG ACAGCCCGAG GAGTTATCTG AAGCTGAAAA CCAGTTCAAG 
AGGATGATTG AACACTACCC CAATGAGGGG CTTGATTGCT TGGCCTACTG TGGAATTGGA 
AAAGTGTATT TGAAAAAAAA CAGATTTTTA GAAGCTCTTA ATCATTTTGA GAAAGCAAGA 
ACCTTGATCT GTCGTCTTCC TGGAGTGTTA ACTTGGCCCA CAAGTAATGT GGTTATTGAG 
GAGACTCAGC CAGGGAAGAT AAAGATGCTG TTAGAGAAAT TTGTTGAAGA ATGCAGGTTC 
CCTCCAGTGC CGGATGCCAC TTGTTGCTAT CAGAAGTGCC ATGGATATTC CAAAATCCAG 
ATATATATAA CTGATCCAGA CTTTAAGGGT TTTATACGGA TCAGCTGTTG CCAGTATTGT 
AAAATAGAAT TTCACATGAA CTGCTGGAAG AAGTTAAAAA CAACAACCTT CAATGATAAA 
ATTGACAAGG ATTTTCTGCA AGGAATCTGT CTTACTCCTG ATTGTGAAGG AATCATTTCA 
AAGATTATCA TCTTCAGCAG TGGTGGTCAA GTTAAATGTG AATTTGAACA CAAGGTCATT 
AAAGAAAAGG TTCCTCCAAG ACCTATTCTG AAACAGAAAT GTTCTAGCCT AGAGAAGTTG 
AGGCTGAAAG AAGACAAAAA ATTGAAGCGA AAGCTCCAAA AAAAGGAAGC AAAAAAATTA 
GCACAAGAAA GACTGGAAGA GGACTTAAGG GAAAGTAACC CACCAAAAAC TGAAGAGCAG 
AGAGAAACTA TCAACCATGT CCAAAGTTGC CAGTTCCTTG ATGACAGAAT TCTGCAGTGC 
ATAAAGCAGT ACGCTGACAA GATTAAGTCC GGGATACTGA ATACTTCCAA GCTTCTCAAA 
GAATTGCTTT CTTGGAAGGT TTTAACCACA GAAGACTACA CAACGTGTTT TTCTAGCAGA 
AATTTCCTGA ATGAAGCAGT GGACTATGTC ATTCGTCATT TGATCCAAGA AAAGAATAGA 
GTAAAGACAA GGGTATTTCT GCATGTTTTG AGTGAGCTTA AAGAAGTGGA CCCAAAACTA 
GCCACCTGGA TCCAGAAACT GAATAGCTTT GGCTTAGATG CCACAGGACC TTTCTTTTCT 
CGTTATGGGG CTCCTCTTAA GGAGCTTGAT TTTAGCATCA TGACTTTTCT CTGGAATGAG 
AAATACGGTC ATAAGCTAGG CTCTATAGAA GGAAAGCAAC TTGATTATTT CTATGAGCCT 
ACATCACTGA AGGAAGCGCG GTGTTTAATA TGGCTGCTAG AAGAACACAG AGACAAGTTC 
CCAGCACTGC ATAATGCCTT GGACGAGTTC TTTGATATAA TGGACAGCCG CTGCACTGTA 
TTAAGGAAAC AGGACAGTGG TGACGTGCCA TTTAATTCTA CCAAGATAAA AAACAAAGGC 
AAGAAAAAGA AGCCAAAAGA TTCAAAGCCT ATGTTAGTTG GATCTGGAAC ACCTTCAGTC 
ACACCAAACA ATGAGACTGT CACTTCAGGT GAAGACCATA TCAAACGCAG TTTAAGTTCT 
GCGGGCCCCT TTGTAGTGCC TGATCACCTT CGGCAGGATA TAGAAGAATT TGAAGCTCTG 
TATGAACAGC ATAGTAATGA GTATGTTGTC CGTAATAAGA AGCTGTGGGA CATAAACCCA 
AAACAGAAAT GTTCAACTCT ATACGATTAT TTCTCTCAGT TGTTGGAGGA ACATGGTCCC 
TTGGACATGA GTAACAAAAT GTTTTCTGAA GAATATGAGT TCTTCCCAGA AGAAACTCAA 
CAGATACTAG AAAAAGCAGG AGGCTTGAAA GCGTTTCTCC TGGGATGTCC TCGTTTTGTT 
GTGATTGATA ACTGTATCGC ATTGAAGAAA GTTGCATTAC GGCTCAAGAA AAAGAGAAAG 
AAGAAAAACA TTAAAACAAA AGTAGAAGAA ATTTCAAAAA CAGGAGAGTA TTTACGAGTT 
AAACTACCAC TGAATCCAAC TGCTAGGGAA TTTAAACCAG ATGTAAAGTC CAAACCAGTG 
TCTGACTTAC CTTCAGCACC AGCTTCTGAG GACATGAAGC CTCAGCCTGC ACCTGCTAAT 
TCTCCCCAAT CAGCTCGTGA CACTGGGAAG CCAAAACCAG TTTTCGATAA TTCTTCCAGA 
TCAGTTTCTG AGGATGAGAA GCCCAAAGGG ATGTCTTCTG ATTCTCCCAG GCCAGTCTTT 
GAGGATACCG GTCACAAGCC GGTCTCCTCT AATTCTCCCA AACCAGTTCC TGAGGAAGTG 
AAAGCGACCT ATTGGCCTCG ACCCCAGTTG GTCACAGGAT ACTGTACATA TCTTCCCTTT 
CGGGGATTTG ACATTACCCA GACTCCACCA GCCTTCATCA GTGTGTTACC GACTTTGCCC 
CAGTATAGCA TTTACACCCC TCTGGCCAGT GTTCCTACTG AGTATCACCT GCAAAGACCA 
ATACCAGTGG TGCCGTCTTT TGTAGCCAGT GACATAACGG AAGTGCATTT TGAGGCTCAT 
CATTTCAGTG CTGAGAATGC CCCTGGTACC CAGATTGCCT CCGAAACACA GATCCTTGAG 
GAATCTTTGG AAACACCAGT GAAGTCAGAG TGCAGCTATG ATGGTGCTGA TATAGTCCAG 
AGTGAGGCTA GCAGAAACGA TGACTACTGT GGCGATTCTG CCAAATGGGA AGTAAATCCA 
GAAAGCACCA ATGAAGTAAC AAATATTCCA TGTACACAAG TGGTTGCTGT ACAGGTGTCT 
TGGAATATAA CACATCAGGA AGTCAATACT GAGCCATATG GTCCTTTTGA GGCACAACAG 
GGGGGTATTT CACAGGTTGA AAAGGAGTAC CAGGTTTTAC AAGAGCAGCT TAAGGAAGCA 
TGTGAAAATT ATGAACACAG CAATGTCAAG GGCTCAGAAG AAACCAAGGA CTTGGAAGAA 
AAGTTGAAAA GGAATGTTGA AGAAAATAAG ATCTCAAAGA CAGAACTAGA TTGGTTCCTT 
GAAGATTTGA ACAAGGAAAT TAAAAAATGG GAACAGGAGA AAAAAGAAAT CCAAGAAAGA 
CTAAAGGCAC TGAAGAAGAA AATGAAAAAG GTGTTAAATG CCAGCAAAAT GTATACGCAG 
GAAAATGATG GAAAGGAAAA GGCACTTGAA TTACTTCTGG ATCAGTCCCT TGAAATCAGT 
AATACATTTA CAAATGAGAA AATGAAAATA GAAGAGAGTA TAAAGAAAGG GAAAGAGCAT 
TATGAAGAGA GTCTTCAAAG GGCTGTGGAT GCAGAGGTAT CTGTGCTTGA AAACTGGAAG 
GACATGGAAG TGTGTAAGTT ACAGAGCATG GAGTCACAAG CCGAAAGGTT TCTCAAGAAT 
CTGAAGCTAA TGAGCAGTGA TTCGGCAGCG TATTCTGACA TGGACTCAGA CATACATTCA 
TGGGAATCGT TTCTTTCTGA TGTTAGAGAA GAAATTGAGA AAGCAAAGTC TCAGTTTGAA 
GAACAAATTC AGGCAATTAA ATATGGTTCT CAGCTCAGTG AACTTCCTAA AGTGCAGATT 
TCTGAGCTTT CATTTCCTAC CTGTAACACG ATTCATCCTA AATTACTTCC TGAGTCTTCA 
GGCCAGGATG GTCAAGGTCC CTCCACTTCT ACAAGTCATG GGACCGGAGA CCAGACAGCA 
GCTCCCGAGG AGTCAAGTCT GGGCTCTGCA GCTGACAGCC CAGTGGAGTT GCCCTCCTGC 
AGTCAGAAAG CCCCCCAGCT GCCAGAGCAT GAGGGTGCTG GCCAGGCAAC TGGGCCAGAA 
CCAGGCCCAG TGGCTGATCA GAAGTTACCT GCTGCTCCAG AGCGTGCCAC TCATTCAAGC 
CAGTCTCCAA AGAAGCCGTT CAATAGCATT ATTGAGCACC TGTTGGTGGT GTTCCCGTGT 
TACAGCAGCA CGGAGCTTGC TGGTTTTATT AAAAAAGTTA GAAACAAAAA CAAGACCTCT 
CTGTCTGGAT TGAGTATTGA CGAAATTGTG CAAAGAGTGA CAGAACACGT CCTTGATGAA 
GAAAGAAAGA AAAAGCCAAG TCCAGGAAAG GGCTGGAGGC TGTCTGCACC CAGCTCTGCT 
GTTGCTGTGC CCAGGGCTGC CCAGGACCCG CCCTCCATGC CTGTCGGAGC ATCGCCCGAA 
ACCAGGGGGC CGAGGACCGA GGACGTCCCT GTGTCCTGCG CAAATTCCTG TGAAATGTGC 
CGAGAAGTAT TCAAGTCCAA ACACGTGCGT GAGCTGCAGT GTGGGCACAG GCTTCACAAG 
GGGTGCTTCA AGCAGTGGCT GAAGAGCCAG CGCACCTGCC CAGCCTGCCT GGGTCACGAC 
CTCCTGTCAG AGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_005202056.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005202113.1 Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X2, mRNA.

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CloneID	OBa218653
Clone ID Related Accession (Same CDS sequence)	XM_005202057.4 , XM_005202056.4 , XM_005202058.4
Accession Version	XM_005202056.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5958bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TTC3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005202056.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGATT TTCCCCAGGG AAATTTTGCA GATTATGCCT TGTTAGAAGA TTGCCCTTAT 
GTGGATGATT GTATCTTTGC CCCTGAGTTT ATGTCCAGTG ATTATGTTCG TGTGACTCAG 
CTTTACTGTG ATGGAGTGGG TAGACAGTAT AAAGATTATG CCCAAACTGA GGGAAACTTA 
GAATTTGACA TCTGCACTCT GTGGTGTAGT CAACCTATTT CTGTCCTGCA AGATTACTGT 
GATGCCATTA AAGTCCACAT CTTCTGGCCA CTTCTGTTTC AACGTCAGCA GAGCTCTATA 
ATAACACGGA TGCACTCCTG TGTAAGGACC AGCGGTTCTC ATGCTTGTGA GATAAGTTTG 
AAGAAATTAC AGCAGCTTGA ACTGATGGAA GATATTGTGG ATTTGGCAAA GAAAGTTGTG 
AATGATACAT TCTTTATCGG AGGCTTATTG AGAATTGGCT ATAAAATAGA GAATAAAATC 
TTGGCAATGG AAGAAGCTTT GAATTGGGTG AAATACACAG GCGATGTAAC AATTTTATCT 
AAATTAGGAG CAGTTGATAA TTGTTGGCGT ATGTTAAGTA TTTTCTTTAC TGAATACAAG 
TATCACATAA CTAAAGTTGT AACAGAAAAC TGCAGTTTGC TTGAAGAATT TAAAACTCAA 
AGTTGTGCGG AGTGTCTAGA GCAAGGAGAA CTAATGAAAA TGAAAGGAAA TGAAGAGTTT 
TCCAAAGAAA GATATGATAT AGCTATTATC TATTACACCA GAGCCATTGA ATGTAGGCCT 
GAAAATCACC TGCTTTATGG TAACCGAGCT CTTTGTTTTC TTCGTACTGG ACAATTCAGA 
AATGCACTTG GTGATGGAAA GAGAGCTACC ATTCTGAAGA ACAATTGGAC AAAGGGTCAT 
TATCGTTATT GTGCTGCTCT TTCTATGCTG GGGGAATATG ACTGGGCCCT GCAAGCAAAC 
ATAAAAGCTC AAAAACTCTG TAAAAATGAC CCTGAGGGAA TCAAGGATCT AATTCAGCAG 
CATGTAAAGT TACAAAAACA AATAGAAGAC CTGCAAGGTC AAACACCAGC TAGTAATCCA 
GTCAAAGCCT TCTGTGAAAG CAGGGCCTAC ATACCTAGTT TATCAGCACC TGCATTTAGT 
ACTTCACTTA ACTTTGTGGA AACTGAAAAA AATTTCAGAA AAACTAACCA CGAAATGGCA 
AACGGTGGTA ATCAGAATCC AAAGGTGGTA GATGAAGCTC TGAAAGTAGA TGATTGTGAC 
TGTCATCCTG AATTTGTACC GCCATCAAGT CAGCCTCCAA AATATAAAGG GAAACAAAAA 
TCTCGAAACA ATGAATTGGA GAAGTTCAGC CCTGGTTCAC AAGAAACTTT ACCAGTAGAT 
TTAAAAAACA TCTTGGAGAA ACAGTTTTCT AAATCTTCCA GAGCCGCACA CCAGGATTTT 
GCTAATATAA TGAAAATGTT GAGAAGTTTA ATTCAGGATG GCTACACGGC CTTGCTGGAG 
CAGCGCTGCC GCAGCGCCGC CCAAGCCTTC ACAGAGTTGC TCAACGGTTT AGATCCTCAA 
AAAATAAAGC AATTAAACCT GGCCATGATT AACTACGTGT TAGTAGTCTA CGGACTTGCC 
ATTTCACTCC TTGGAATAGG ACAGCCCGAG GAGTTATCTG AAGCTGAAAA CCAGTTCAAG 
AGGATGATTG AACACTACCC CAATGAGGGG CTTGATTGCT TGGCCTACTG TGGAATTGGA 
AAAGTGTATT TGAAAAAAAA CAGATTTTTA GAAGCTCTTA ATCATTTTGA GAAAGCAAGA 
ACCTTGATCT GTCGTCTTCC TGGAGTGTTA ACTTGGCCCA CAAGTAATGT GGTTATTGAG 
GAGACTCAGC CAGGGAAGAT AAAGATGCTG TTAGAGAAAT TTGTTGAAGA ATGCAGGTTC 
CCTCCAGTGC CGGATGCCAC TTGTTGCTAT CAGAAGTGCC ATGGATATTC CAAAATCCAG 
ATATATATAA CTGATCCAGA CTTTAAGGGT TTTATACGGA TCAGCTGTTG CCAGTATTGT 
AAAATAGAAT TTCACATGAA CTGCTGGAAG AAGTTAAAAA CAACAACCTT CAATGATAAA 
ATTGACAAGG ATTTTCTGCA AGGAATCTGT CTTACTCCTG ATTGTGAAGG AATCATTTCA 
AAGATTATCA TCTTCAGCAG TGGTGGTCAA GTTAAATGTG AATTTGAACA CAAGGTCATT 
AAAGAAAAGG TTCCTCCAAG ACCTATTCTG AAACAGAAAT GTTCTAGCCT AGAGAAGTTG 
AGGCTGAAAG AAGACAAAAA ATTGAAGCGA AAGCTCCAAA AAAAGGAAGC AAAAAAATTA 
GCACAAGAAA GACTGGAAGA GGACTTAAGG GAAAGTAACC CACCAAAAAC TGAAGAGCAG 
AGAGAAACTA TCAACCATGT CCAAAGTTGC CAGTTCCTTG ATGACAGAAT TCTGCAGTGC 
ATAAAGCAGT ACGCTGACAA GATTAAGTCC GGGATACTGA ATACTTCCAA GCTTCTCAAA 
GAATTGCTTT CTTGGAAGGT TTTAACCACA GAAGACTACA CAACGTGTTT TTCTAGCAGA 
AATTTCCTGA ATGAAGCAGT GGACTATGTC ATTCGTCATT TGATCCAAGA AAAGAATAGA 
GTAAAGACAA GGGTATTTCT GCATGTTTTG AGTGAGCTTA AAGAAGTGGA CCCAAAACTA 
GCCACCTGGA TCCAGAAACT GAATAGCTTT GGCTTAGATG CCACAGGACC TTTCTTTTCT 
CGTTATGGGG CTCCTCTTAA GGAGCTTGAT TTTAGCATCA TGACTTTTCT CTGGAATGAG 
AAATACGGTC ATAAGCTAGG CTCTATAGAA GGAAAGCAAC TTGATTATTT CTATGAGCCT 
ACATCACTGA AGGAAGCGCG GTGTTTAATA TGGCTGCTAG AAGAACACAG AGACAAGTTC 
CCAGCACTGC ATAATGCCTT GGACGAGTTC TTTGATATAA TGGACAGCCG CTGCACTGTA 
TTAAGGAAAC AGGACAGTGG TGACGTGCCA TTTAATTCTA CCAAGATAAA AAACAAAGGC 
AAGAAAAAGA AGCCAAAAGA TTCAAAGCCT ATGTTAGTTG GATCTGGAAC ACCTTCAGTC 
ACACCAAACA ATGAGACTGT CACTTCAGGT GAAGACCATA TCAAACGCAG TTTAAGTTCT 
GCGGGCCCCT TTGTAGTGCC TGATCACCTT CGGCAGGATA TAGAAGAATT TGAAGCTCTG 
TATGAACAGC ATAGTAATGA GTATGTTGTC CGTAATAAGA AGCTGTGGGA CATAAACCCA 
AAACAGAAAT GTTCAACTCT ATACGATTAT TTCTCTCAGT TGTTGGAGGA ACATGGTCCC 
TTGGACATGA GTAACAAAAT GTTTTCTGAA GAATATGAGT TCTTCCCAGA AGAAACTCAA 
CAGATACTAG AAAAAGCAGG AGGCTTGAAA GCGTTTCTCC TGGGATGTCC TCGTTTTGTT 
GTGATTGATA ACTGTATCGC ATTGAAGAAA GTTGCATTAC GGCTCAAGAA AAAGAGAAAG 
AAGAAAAACA TTAAAACAAA AGTAGAAGAA ATTTCAAAAA CAGGAGAGTA TTTACGAGTT 
AAACTACCAC TGAATCCAAC TGCTAGGGAA TTTAAACCAG ATGTAAAGTC CAAACCAGTG 
TCTGACTTAC CTTCAGCACC AGCTTCTGAG GACATGAAGC CTCAGCCTGC ACCTGCTAAT 
TCTCCCCAAT CAGCTCGTGA CACTGGGAAG CCAAAACCAG TTTTCGATAA TTCTTCCAGA 
TCAGTTTCTG AGGATGAGAA GCCCAAAGGG ATGTCTTCTG ATTCTCCCAG GCCAGTCTTT 
GAGGATACCG GTCACAAGCC GGTCTCCTCT AATTCTCCCA AACCAGTTCC TGAGGAAGTG 
AAAGCGACCT ATTGGCCTCG ACCCCAGTTG GTCACAGGAT ACTGTACATA TCTTCCCTTT 
CGGGGATTTG ACATTACCCA GACTCCACCA GCCTTCATCA GTGTGTTACC GACTTTGCCC 
CAGTATAGCA TTTACACCCC TCTGGCCAGT GTTCCTACTG AGTATCACCT GCAAAGACCA 
ATACCAGTGG TGCCGTCTTT TGTAGCCAGT GACATAACGG AAGTGCATTT TGAGGCTCAT 
CATTTCAGTG CTGAGAATGC CCCTGGTACC CAGATTGCCT CCGAAACACA GATCCTTGAG 
GAATCTTTGG AAACACCAGT GAAGTCAGAG TGCAGCTATG ATGGTGCTGA TATAGTCCAG 
AGTGAGGCTA GCAGAAACGA TGACTACTGT GGCGATTCTG CCAAATGGGA AGTAAATCCA 
GAAAGCACCA ATGAAGTAAC AAATATTCCA TGTACACAAG TGGTTGCTGT ACAGGTGTCT 
TGGAATATAA CACATCAGGA AGTCAATACT GAGCCATATG GTCCTTTTGA GGCACAACAG 
GGGGGTATTT CACAGGTTGA AAAGGAGTAC CAGGTTTTAC AAGAGCAGCT TAAGGAAGCA 
TGTGAAAATT ATGAACACAG CAATGTCAAG GGCTCAGAAG AAACCAAGGA CTTGGAAGAA 
AAGTTGAAAA GGAATGTTGA AGAAAATAAG ATCTCAAAGA CAGAACTAGA TTGGTTCCTT 
GAAGATTTGA ACAAGGAAAT TAAAAAATGG GAACAGGAGA AAAAAGAAAT CCAAGAAAGA 
CTAAAGGCAC TGAAGAAGAA AATGAAAAAG GTGTTAAATG CCAGCAAAAT GTATACGCAG 
GAAAATGATG GAAAGGAAAA GGCACTTGAA TTACTTCTGG ATCAGTCCCT TGAAATCAGT 
AATACATTTA CAAATGAGAA AATGAAAATA GAAGAGAGTA TAAAGAAAGG GAAAGAGCAT 
TATGAAGAGA GTCTTCAAAG GGCTGTGGAT GCAGAGGTAT CTGTGCTTGA AAACTGGAAG 
GACATGGAAG TGTGTAAGTT ACAGAGCATG GAGTCACAAG CCGAAAGGTT TCTCAAGAAT 
CTGAAGCTAA TGAGCAGTGA TTCGGCAGCG TATTCTGACA TGGACTCAGA CATACATTCA 
TGGGAATCGT TTCTTTCTGA TGTTAGAGAA GAAATTGAGA AAGCAAAGTC TCAGTTTGAA 
GAACAAATTC AGGCAATTAA ATATGGTTCT CAGCTCAGTG AACTTCCTAA AGTGCAGATT 
TCTGAGCTTT CATTTCCTAC CTGTAACACG ATTCATCCTA AATTACTTCC TGAGTCTTCA 
GGCCAGGATG GTCAAGGTCC CTCCACTTCT ACAAGTCATG GGACCGGAGA CCAGACAGCA 
GCTCCCGAGG AGTCAAGTCT GGGCTCTGCA GCTGACAGCC CAGTGGAGTT GCCCTCCTGC 
AGTCAGAAAG CCCCCCAGCT GCCAGAGCAT GAGGGTGCTG GCCAGGCAAC TGGGCCAGAA 
CCAGGCCCAG TGGCTGATCA GAAGTTACCT GCTGCTCCAG AGCGTGCCAC TCATTCAAGC 
CAGTCTCCAA AGAAGCCGTT CAATAGCATT ATTGAGCACC TGTTGGTGGT GTTCCCGTGT 
TACAGCAGCA CGGAGCTTGC TGGTTTTATT AAAAAAGTTA GAAACAAAAA CAAGACCTCT 
CTGTCTGGAT TGAGTATTGA CGAAATTGTG CAAAGAGTGA CAGAACACGT CCTTGATGAA 
GAAAGAAAGA AAAAGCCAAG TCCAGGAAAG GGCTGGAGGC TGTCTGCACC CAGCTCTGCT 
GTTGCTGTGC CCAGGGCTGC CCAGGACCCG CCCTCCATGC CTGTCGGAGC ATCGCCCGAA 
ACCAGGGGGC CGAGGACCGA GGACGTCCCT GTGTCCTGCG CAAATTCCTG TGAAATGTGC 
CGAGAAGTAT TCAAGTCCAA ACACGTGCGT GAGCTGCAGT GTGGGCACAG GCTTCACAAG 
GGGTGCTTCA AGCAGTGGCT GAAGAGCCAG CGCACCTGCC CAGCCTGCCT GGGTCACGAC 
CTCCTGTCAG AGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005202113.1
CDS	255..6212
Translation	MADFPQGNFADYALLEDCPYVDDCIFAPEFMSSDYVRVTQLYCDGVGRQYKDYAQTEGNLEFDICTLWCSQPISVLQDYCDAIKVHIFWPLLFQRQQSSIITRMHSCVRTSGSHACEISLKKLQQLELMEDIVDLAKKVVNDTFFIGGLLRIGYKIENKILAMEEALNWVKYTGDVTILSKLGAVDNCWRMLSIFFTEYKYHITKVVTENCSLLEEFKTQSCAECLEQGELMKMKGNEEFSKERYDIAIIYYTRAIECRPENHLLYGNRALCFLRTGQFRNALGDGKRATILKNNWTKGHYRYCAALSMLGEYDWALQANIKAQKLCKNDPEGIKDLIQQHVKLQKQIEDLQGQTPASNPVKAFCESRAYIPSLSAPAFSTSLNFVETEKNFRKTNHEMANGGNQNPKVVDEALKVDDCDCHPEFVPPSSQPPKYKGKQKSRNNELEKFSPGSQETLPVDLKNILEKQFSKSSRAAHQDFANIMKMLRSLIQDGYTALLEQRCRSAAQAFTELLNGLDPQKIKQLNLAMINYVLVVYGLAISLLGIGQPEELSEAENQFKRMIEHYPNEGLDCLAYCGIGKVYLKKNRFLEALNHFEKARTLICRLPGVLTWPTSNVVIEETQPGKIKMLLEKFVEECRFPPVPDATCCYQKCHGYSKIQIYITDPDFKGFIRISCCQYCKIEFHMNCWKKLKTTTFNDKIDKDFLQGICLTPDCEGIISKIIIFSSGGQVKCEFEHKVIKEKVPPRPILKQKCSSLEKLRLKEDKKLKRKLQKKEAKKLAQERLEEDLRESNPPKTEEQRETINHVQSCQFLDDRILQCIKQYADKIKSGILNTSKLLKELLSWKVLTTEDYTTCFSSRNFLNEAVDYVIRHLIQEKNRVKTRVFLHVLSELKEVDPKLATWIQKLNSFGLDATGPFFSRYGAPLKELDFSIMTFLWNEKYGHKLGSIEGKQLDYFYEPTSLKEARCLIWLLEEHRDKFPALHNALDEFFDIMDSRCTVLRKQDSGDVPFNSTKIKNKGKKKKPKDSKPMLVGSGTPSVTPNNETVTSGEDHIKRSLSSAGPFVVPDHLRQDIEEFEALYEQHSNEYVVRNKKLWDINPKQKCSTLYDYFSQLLEEHGPLDMSNKMFSEEYEFFPEETQQILEKAGGLKAFLLGCPRFVVIDNCIALKKVALRLKKKRKKKNIKTKVEEISKTGEYLRVKLPLNPTAREFKPDVKSKPVSDLPSAPASEDMKPQPAPANSPQSARDTGKPKPVFDNSSRSVSEDEKPKGMSSDSPRPVFEDTGHKPVSSNSPKPVPEEVKATYWPRPQLVTGYCTYLPFRGFDITQTPPAFISVLPTLPQYSIYTPLASVPTEYHLQRPIPVVPSFVASDITEVHFEAHHFSAENAPGTQIASETQILEESLETPVKSECSYDGADIVQSEASRNDDYCGDSAKWEVNPESTNEVTNIPCTQVVAVQVSWNITHQEVNTEPYGPFEAQQGGISQVEKEYQVLQEQLKEACENYEHSNVKGSEETKDLEEKLKRNVEENKISKTELDWFLEDLNKEIKKWEQEKKEIQERLKALKKKMKKVLNASKMYTQENDGKEKALELLLDQSLEISNTFTNEKMKIEESIKKGKEHYEESLQRAVDAEVSVLENWKDMEVCKLQSMESQAERFLKNLKLMSSDSAAYSDMDSDIHSWESFLSDVREEIEKAKSQFEEQIQAIKYGSQLSELPKVQISELSFPTCNTIHPKLLPESSGQDGQGPSTSTSHGTGDQTAAPEESSLGSAADSPVELPSCSQKAPQLPEHEGAGQATGPEPGPVADQKLPAAPERATHSSQSPKKPFNSIIEHLLVVFPCYSSTELAGFIKKVRNKNKTSLSGLSIDEIVQRVTEHVLDEERKKKPSPGKGWRLSAPSSAVAVPRAAQDPPSMPVGASPETRGPRTEDVPVSCANSCEMCREVFKSKHVRELQCGHRLHKGCFKQWLKSQRTCPACLGHDLLSEE

Target ORF information:

RefSeq Version	XM_005202056.4
Organism	Bos taurus(cattle)
Definition	Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005202056.4

ORF Insert Sequence:

ATGGCCGATT TTCCCCAGGG AAATTTTGCA GATTATGCCT TGTTAGAAGA TTGCCCTTAT 
GTGGATGATT GTATCTTTGC CCCTGAGTTT ATGTCCAGTG ATTATGTTCG TGTGACTCAG 
CTTTACTGTG ATGGAGTGGG TAGACAGTAT AAAGATTATG CCCAAACTGA GGGAAACTTA 
GAATTTGACA TCTGCACTCT GTGGTGTAGT CAACCTATTT CTGTCCTGCA AGATTACTGT 
GATGCCATTA AAGTCCACAT CTTCTGGCCA CTTCTGTTTC AACGTCAGCA GAGCTCTATA 
ATAACACGGA TGCACTCCTG TGTAAGGACC AGCGGTTCTC ATGCTTGTGA GATAAGTTTG 
AAGAAATTAC AGCAGCTTGA ACTGATGGAA GATATTGTGG ATTTGGCAAA GAAAGTTGTG 
AATGATACAT TCTTTATCGG AGGCTTATTG AGAATTGGCT ATAAAATAGA GAATAAAATC 
TTGGCAATGG AAGAAGCTTT GAATTGGGTG AAATACACAG GCGATGTAAC AATTTTATCT 
AAATTAGGAG CAGTTGATAA TTGTTGGCGT ATGTTAAGTA TTTTCTTTAC TGAATACAAG 
TATCACATAA CTAAAGTTGT AACAGAAAAC TGCAGTTTGC TTGAAGAATT TAAAACTCAA 
AGTTGTGCGG AGTGTCTAGA GCAAGGAGAA CTAATGAAAA TGAAAGGAAA TGAAGAGTTT 
TCCAAAGAAA GATATGATAT AGCTATTATC TATTACACCA GAGCCATTGA ATGTAGGCCT 
GAAAATCACC TGCTTTATGG TAACCGAGCT CTTTGTTTTC TTCGTACTGG ACAATTCAGA 
AATGCACTTG GTGATGGAAA GAGAGCTACC ATTCTGAAGA ACAATTGGAC AAAGGGTCAT 
TATCGTTATT GTGCTGCTCT TTCTATGCTG GGGGAATATG ACTGGGCCCT GCAAGCAAAC 
ATAAAAGCTC AAAAACTCTG TAAAAATGAC CCTGAGGGAA TCAAGGATCT AATTCAGCAG 
CATGTAAAGT TACAAAAACA AATAGAAGAC CTGCAAGGTC AAACACCAGC TAGTAATCCA 
GTCAAAGCCT TCTGTGAAAG CAGGGCCTAC ATACCTAGTT TATCAGCACC TGCATTTAGT 
ACTTCACTTA ACTTTGTGGA AACTGAAAAA AATTTCAGAA AAACTAACCA CGAAATGGCA 
AACGGTGGTA ATCAGAATCC AAAGGTGGTA GATGAAGCTC TGAAAGTAGA TGATTGTGAC 
TGTCATCCTG AATTTGTACC GCCATCAAGT CAGCCTCCAA AATATAAAGG GAAACAAAAA 
TCTCGAAACA ATGAATTGGA GAAGTTCAGC CCTGGTTCAC AAGAAACTTT ACCAGTAGAT 
TTAAAAAACA TCTTGGAGAA ACAGTTTTCT AAATCTTCCA GAGCCGCACA CCAGGATTTT 
GCTAATATAA TGAAAATGTT GAGAAGTTTA ATTCAGGATG GCTACACGGC CTTGCTGGAG 
CAGCGCTGCC GCAGCGCCGC CCAAGCCTTC ACAGAGTTGC TCAACGGTTT AGATCCTCAA 
AAAATAAAGC AATTAAACCT GGCCATGATT AACTACGTGT TAGTAGTCTA CGGACTTGCC 
ATTTCACTCC TTGGAATAGG ACAGCCCGAG GAGTTATCTG AAGCTGAAAA CCAGTTCAAG 
AGGATGATTG AACACTACCC CAATGAGGGG CTTGATTGCT TGGCCTACTG TGGAATTGGA 
AAAGTGTATT TGAAAAAAAA CAGATTTTTA GAAGCTCTTA ATCATTTTGA GAAAGCAAGA 
ACCTTGATCT GTCGTCTTCC TGGAGTGTTA ACTTGGCCCA CAAGTAATGT GGTTATTGAG 
GAGACTCAGC CAGGGAAGAT AAAGATGCTG TTAGAGAAAT TTGTTGAAGA ATGCAGGTTC 
CCTCCAGTGC CGGATGCCAC TTGTTGCTAT CAGAAGTGCC ATGGATATTC CAAAATCCAG 
ATATATATAA CTGATCCAGA CTTTAAGGGT TTTATACGGA TCAGCTGTTG CCAGTATTGT 
AAAATAGAAT TTCACATGAA CTGCTGGAAG AAGTTAAAAA CAACAACCTT CAATGATAAA 
ATTGACAAGG ATTTTCTGCA AGGAATCTGT CTTACTCCTG ATTGTGAAGG AATCATTTCA 
AAGATTATCA TCTTCAGCAG TGGTGGTCAA GTTAAATGTG AATTTGAACA CAAGGTCATT 
AAAGAAAAGG TTCCTCCAAG ACCTATTCTG AAACAGAAAT GTTCTAGCCT AGAGAAGTTG 
AGGCTGAAAG AAGACAAAAA ATTGAAGCGA AAGCTCCAAA AAAAGGAAGC AAAAAAATTA 
GCACAAGAAA GACTGGAAGA GGACTTAAGG GAAAGTAACC CACCAAAAAC TGAAGAGCAG 
AGAGAAACTA TCAACCATGT CCAAAGTTGC CAGTTCCTTG ATGACAGAAT TCTGCAGTGC 
ATAAAGCAGT ACGCTGACAA GATTAAGTCC GGGATACTGA ATACTTCCAA GCTTCTCAAA 
GAATTGCTTT CTTGGAAGGT TTTAACCACA GAAGACTACA CAACGTGTTT TTCTAGCAGA 
AATTTCCTGA ATGAAGCAGT GGACTATGTC ATTCGTCATT TGATCCAAGA AAAGAATAGA 
GTAAAGACAA GGGTATTTCT GCATGTTTTG AGTGAGCTTA AAGAAGTGGA CCCAAAACTA 
GCCACCTGGA TCCAGAAACT GAATAGCTTT GGCTTAGATG CCACAGGACC TTTCTTTTCT 
CGTTATGGGG CTCCTCTTAA GGAGCTTGAT TTTAGCATCA TGACTTTTCT CTGGAATGAG 
AAATACGGTC ATAAGCTAGG CTCTATAGAA GGAAAGCAAC TTGATTATTT CTATGAGCCT 
ACATCACTGA AGGAAGCGCG GTGTTTAATA TGGCTGCTAG AAGAACACAG AGACAAGTTC 
CCAGCACTGC ATAATGCCTT GGACGAGTTC TTTGATATAA TGGACAGCCG CTGCACTGTA 
TTAAGGAAAC AGGACAGTGG TGACGTGCCA TTTAATTCTA CCAAGATAAA AAACAAAGGC 
AAGAAAAAGA AGCCAAAAGA TTCAAAGCCT ATGTTAGTTG GATCTGGAAC ACCTTCAGTC 
ACACCAAACA ATGAGACTGT CACTTCAGGT GAAGACCATA TCAAACGCAG TTTAAGTTCT 
GCGGGCCCCT TTGTAGTGCC TGATCACCTT CGGCAGGATA TAGAAGAATT TGAAGCTCTG 
TATGAACAGC ATAGTAATGA GTATGTTGTC CGTAATAAGA AGCTGTGGGA CATAAACCCA 
AAACAGAAAT GTTCAACTCT ATACGATTAT TTCTCTCAGT TGTTGGAGGA ACATGGTCCC 
TTGGACATGA GTAACAAAAT GTTTTCTGAA GAATATGAGT TCTTCCCAGA AGAAACTCAA 
CAGATACTAG AAAAAGCAGG AGGCTTGAAA GCGTTTCTCC TGGGATGTCC TCGTTTTGTT 
GTGATTGATA ACTGTATCGC ATTGAAGAAA GTTGCATTAC GGCTCAAGAA AAAGAGAAAG 
AAGAAAAACA TTAAAACAAA AGTAGAAGAA ATTTCAAAAA CAGGAGAGTA TTTACGAGTT 
AAACTACCAC TGAATCCAAC TGCTAGGGAA TTTAAACCAG ATGTAAAGTC CAAACCAGTG 
TCTGACTTAC CTTCAGCACC AGCTTCTGAG GACATGAAGC CTCAGCCTGC ACCTGCTAAT 
TCTCCCCAAT CAGCTCGTGA CACTGGGAAG CCAAAACCAG TTTTCGATAA TTCTTCCAGA 
TCAGTTTCTG AGGATGAGAA GCCCAAAGGG ATGTCTTCTG ATTCTCCCAG GCCAGTCTTT 
GAGGATACCG GTCACAAGCC GGTCTCCTCT AATTCTCCCA AACCAGTTCC TGAGGAAGTG 
AAAGCGACCT ATTGGCCTCG ACCCCAGTTG GTCACAGGAT ACTGTACATA TCTTCCCTTT 
CGGGGATTTG ACATTACCCA GACTCCACCA GCCTTCATCA GTGTGTTACC GACTTTGCCC 
CAGTATAGCA TTTACACCCC TCTGGCCAGT GTTCCTACTG AGTATCACCT GCAAAGACCA 
ATACCAGTGG TGCCGTCTTT TGTAGCCAGT GACATAACGG AAGTGCATTT TGAGGCTCAT 
CATTTCAGTG CTGAGAATGC CCCTGGTACC CAGATTGCCT CCGAAACACA GATCCTTGAG 
GAATCTTTGG AAACACCAGT GAAGTCAGAG TGCAGCTATG ATGGTGCTGA TATAGTCCAG 
AGTGAGGCTA GCAGAAACGA TGACTACTGT GGCGATTCTG CCAAATGGGA AGTAAATCCA 
GAAAGCACCA ATGAAGTAAC AAATATTCCA TGTACACAAG TGGTTGCTGT ACAGGTGTCT 
TGGAATATAA CACATCAGGA AGTCAATACT GAGCCATATG GTCCTTTTGA GGCACAACAG 
GGGGGTATTT CACAGGTTGA AAAGGAGTAC CAGGTTTTAC AAGAGCAGCT TAAGGAAGCA 
TGTGAAAATT ATGAACACAG CAATGTCAAG GGCTCAGAAG AAACCAAGGA CTTGGAAGAA 
AAGTTGAAAA GGAATGTTGA AGAAAATAAG ATCTCAAAGA CAGAACTAGA TTGGTTCCTT 
GAAGATTTGA ACAAGGAAAT TAAAAAATGG GAACAGGAGA AAAAAGAAAT CCAAGAAAGA 
CTAAAGGCAC TGAAGAAGAA AATGAAAAAG GTGTTAAATG CCAGCAAAAT GTATACGCAG 
GAAAATGATG GAAAGGAAAA GGCACTTGAA TTACTTCTGG ATCAGTCCCT TGAAATCAGT 
AATACATTTA CAAATGAGAA AATGAAAATA GAAGAGAGTA TAAAGAAAGG GAAAGAGCAT 
TATGAAGAGA GTCTTCAAAG GGCTGTGGAT GCAGAGGTAT CTGTGCTTGA AAACTGGAAG 
GACATGGAAG TGTGTAAGTT ACAGAGCATG GAGTCACAAG CCGAAAGGTT TCTCAAGAAT 
CTGAAGCTAA TGAGCAGTGA TTCGGCAGCG TATTCTGACA TGGACTCAGA CATACATTCA 
TGGGAATCGT TTCTTTCTGA TGTTAGAGAA GAAATTGAGA AAGCAAAGTC TCAGTTTGAA 
GAACAAATTC AGGCAATTAA ATATGGTTCT CAGCTCAGTG AACTTCCTAA AGTGCAGATT 
TCTGAGCTTT CATTTCCTAC CTGTAACACG ATTCATCCTA AATTACTTCC TGAGTCTTCA 
GGCCAGGATG GTCAAGGTCC CTCCACTTCT ACAAGTCATG GGACCGGAGA CCAGACAGCA 
GCTCCCGAGG AGTCAAGTCT GGGCTCTGCA GCTGACAGCC CAGTGGAGTT GCCCTCCTGC 
AGTCAGAAAG CCCCCCAGCT GCCAGAGCAT GAGGGTGCTG GCCAGGCAAC TGGGCCAGAA 
CCAGGCCCAG TGGCTGATCA GAAGTTACCT GCTGCTCCAG AGCGTGCCAC TCATTCAAGC 
CAGTCTCCAA AGAAGCCGTT CAATAGCATT ATTGAGCACC TGTTGGTGGT GTTCCCGTGT 
TACAGCAGCA CGGAGCTTGC TGGTTTTATT AAAAAAGTTA GAAACAAAAA CAAGACCTCT 
CTGTCTGGAT TGAGTATTGA CGAAATTGTG CAAAGAGTGA CAGAACACGT CCTTGATGAA 
GAAAGAAAGA AAAAGCCAAG TCCAGGAAAG GGCTGGAGGC TGTCTGCACC CAGCTCTGCT 
GTTGCTGTGC CCAGGGCTGC CCAGGACCCG CCCTCCATGC CTGTCGGAGC ATCGCCCGAA 
ACCAGGGGGC CGAGGACCGA GGACGTCCCT GTGTCCTGCG CAAATTCCTG TGAAATGTGC 
CGAGAAGTAT TCAAGTCCAA ACACGTGCGT GAGCTGCAGT GTGGGCACAG GCTTCACAAG 
GGGTGCTTCA AGCAGTGGCT GAAGAGCCAG CGCACCTGCC CAGCCTGCCT GGGTCACGAC 
CTCCTGTCAG AGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_005202058.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005202115.1 Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X3, mRNA.

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CloneID	OBa218653
Clone ID Related Accession (Same CDS sequence)	XM_005202057.4 , XM_005202056.4 , XM_005202058.4
Accession Version	XM_005202058.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5958bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TTC3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005202058.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGATT TTCCCCAGGG AAATTTTGCA GATTATGCCT TGTTAGAAGA TTGCCCTTAT 
GTGGATGATT GTATCTTTGC CCCTGAGTTT ATGTCCAGTG ATTATGTTCG TGTGACTCAG 
CTTTACTGTG ATGGAGTGGG TAGACAGTAT AAAGATTATG CCCAAACTGA GGGAAACTTA 
GAATTTGACA TCTGCACTCT GTGGTGTAGT CAACCTATTT CTGTCCTGCA AGATTACTGT 
GATGCCATTA AAGTCCACAT CTTCTGGCCA CTTCTGTTTC AACGTCAGCA GAGCTCTATA 
ATAACACGGA TGCACTCCTG TGTAAGGACC AGCGGTTCTC ATGCTTGTGA GATAAGTTTG 
AAGAAATTAC AGCAGCTTGA ACTGATGGAA GATATTGTGG ATTTGGCAAA GAAAGTTGTG 
AATGATACAT TCTTTATCGG AGGCTTATTG AGAATTGGCT ATAAAATAGA GAATAAAATC 
TTGGCAATGG AAGAAGCTTT GAATTGGGTG AAATACACAG GCGATGTAAC AATTTTATCT 
AAATTAGGAG CAGTTGATAA TTGTTGGCGT ATGTTAAGTA TTTTCTTTAC TGAATACAAG 
TATCACATAA CTAAAGTTGT AACAGAAAAC TGCAGTTTGC TTGAAGAATT TAAAACTCAA 
AGTTGTGCGG AGTGTCTAGA GCAAGGAGAA CTAATGAAAA TGAAAGGAAA TGAAGAGTTT 
TCCAAAGAAA GATATGATAT AGCTATTATC TATTACACCA GAGCCATTGA ATGTAGGCCT 
GAAAATCACC TGCTTTATGG TAACCGAGCT CTTTGTTTTC TTCGTACTGG ACAATTCAGA 
AATGCACTTG GTGATGGAAA GAGAGCTACC ATTCTGAAGA ACAATTGGAC AAAGGGTCAT 
TATCGTTATT GTGCTGCTCT TTCTATGCTG GGGGAATATG ACTGGGCCCT GCAAGCAAAC 
ATAAAAGCTC AAAAACTCTG TAAAAATGAC CCTGAGGGAA TCAAGGATCT AATTCAGCAG 
CATGTAAAGT TACAAAAACA AATAGAAGAC CTGCAAGGTC AAACACCAGC TAGTAATCCA 
GTCAAAGCCT TCTGTGAAAG CAGGGCCTAC ATACCTAGTT TATCAGCACC TGCATTTAGT 
ACTTCACTTA ACTTTGTGGA AACTGAAAAA AATTTCAGAA AAACTAACCA CGAAATGGCA 
AACGGTGGTA ATCAGAATCC AAAGGTGGTA GATGAAGCTC TGAAAGTAGA TGATTGTGAC 
TGTCATCCTG AATTTGTACC GCCATCAAGT CAGCCTCCAA AATATAAAGG GAAACAAAAA 
TCTCGAAACA ATGAATTGGA GAAGTTCAGC CCTGGTTCAC AAGAAACTTT ACCAGTAGAT 
TTAAAAAACA TCTTGGAGAA ACAGTTTTCT AAATCTTCCA GAGCCGCACA CCAGGATTTT 
GCTAATATAA TGAAAATGTT GAGAAGTTTA ATTCAGGATG GCTACACGGC CTTGCTGGAG 
CAGCGCTGCC GCAGCGCCGC CCAAGCCTTC ACAGAGTTGC TCAACGGTTT AGATCCTCAA 
AAAATAAAGC AATTAAACCT GGCCATGATT AACTACGTGT TAGTAGTCTA CGGACTTGCC 
ATTTCACTCC TTGGAATAGG ACAGCCCGAG GAGTTATCTG AAGCTGAAAA CCAGTTCAAG 
AGGATGATTG AACACTACCC CAATGAGGGG CTTGATTGCT TGGCCTACTG TGGAATTGGA 
AAAGTGTATT TGAAAAAAAA CAGATTTTTA GAAGCTCTTA ATCATTTTGA GAAAGCAAGA 
ACCTTGATCT GTCGTCTTCC TGGAGTGTTA ACTTGGCCCA CAAGTAATGT GGTTATTGAG 
GAGACTCAGC CAGGGAAGAT AAAGATGCTG TTAGAGAAAT TTGTTGAAGA ATGCAGGTTC 
CCTCCAGTGC CGGATGCCAC TTGTTGCTAT CAGAAGTGCC ATGGATATTC CAAAATCCAG 
ATATATATAA CTGATCCAGA CTTTAAGGGT TTTATACGGA TCAGCTGTTG CCAGTATTGT 
AAAATAGAAT TTCACATGAA CTGCTGGAAG AAGTTAAAAA CAACAACCTT CAATGATAAA 
ATTGACAAGG ATTTTCTGCA AGGAATCTGT CTTACTCCTG ATTGTGAAGG AATCATTTCA 
AAGATTATCA TCTTCAGCAG TGGTGGTCAA GTTAAATGTG AATTTGAACA CAAGGTCATT 
AAAGAAAAGG TTCCTCCAAG ACCTATTCTG AAACAGAAAT GTTCTAGCCT AGAGAAGTTG 
AGGCTGAAAG AAGACAAAAA ATTGAAGCGA AAGCTCCAAA AAAAGGAAGC AAAAAAATTA 
GCACAAGAAA GACTGGAAGA GGACTTAAGG GAAAGTAACC CACCAAAAAC TGAAGAGCAG 
AGAGAAACTA TCAACCATGT CCAAAGTTGC CAGTTCCTTG ATGACAGAAT TCTGCAGTGC 
ATAAAGCAGT ACGCTGACAA GATTAAGTCC GGGATACTGA ATACTTCCAA GCTTCTCAAA 
GAATTGCTTT CTTGGAAGGT TTTAACCACA GAAGACTACA CAACGTGTTT TTCTAGCAGA 
AATTTCCTGA ATGAAGCAGT GGACTATGTC ATTCGTCATT TGATCCAAGA AAAGAATAGA 
GTAAAGACAA GGGTATTTCT GCATGTTTTG AGTGAGCTTA AAGAAGTGGA CCCAAAACTA 
GCCACCTGGA TCCAGAAACT GAATAGCTTT GGCTTAGATG CCACAGGACC TTTCTTTTCT 
CGTTATGGGG CTCCTCTTAA GGAGCTTGAT TTTAGCATCA TGACTTTTCT CTGGAATGAG 
AAATACGGTC ATAAGCTAGG CTCTATAGAA GGAAAGCAAC TTGATTATTT CTATGAGCCT 
ACATCACTGA AGGAAGCGCG GTGTTTAATA TGGCTGCTAG AAGAACACAG AGACAAGTTC 
CCAGCACTGC ATAATGCCTT GGACGAGTTC TTTGATATAA TGGACAGCCG CTGCACTGTA 
TTAAGGAAAC AGGACAGTGG TGACGTGCCA TTTAATTCTA CCAAGATAAA AAACAAAGGC 
AAGAAAAAGA AGCCAAAAGA TTCAAAGCCT ATGTTAGTTG GATCTGGAAC ACCTTCAGTC 
ACACCAAACA ATGAGACTGT CACTTCAGGT GAAGACCATA TCAAACGCAG TTTAAGTTCT 
GCGGGCCCCT TTGTAGTGCC TGATCACCTT CGGCAGGATA TAGAAGAATT TGAAGCTCTG 
TATGAACAGC ATAGTAATGA GTATGTTGTC CGTAATAAGA AGCTGTGGGA CATAAACCCA 
AAACAGAAAT GTTCAACTCT ATACGATTAT TTCTCTCAGT TGTTGGAGGA ACATGGTCCC 
TTGGACATGA GTAACAAAAT GTTTTCTGAA GAATATGAGT TCTTCCCAGA AGAAACTCAA 
CAGATACTAG AAAAAGCAGG AGGCTTGAAA GCGTTTCTCC TGGGATGTCC TCGTTTTGTT 
GTGATTGATA ACTGTATCGC ATTGAAGAAA GTTGCATTAC GGCTCAAGAA AAAGAGAAAG 
AAGAAAAACA TTAAAACAAA AGTAGAAGAA ATTTCAAAAA CAGGAGAGTA TTTACGAGTT 
AAACTACCAC TGAATCCAAC TGCTAGGGAA TTTAAACCAG ATGTAAAGTC CAAACCAGTG 
TCTGACTTAC CTTCAGCACC AGCTTCTGAG GACATGAAGC CTCAGCCTGC ACCTGCTAAT 
TCTCCCCAAT CAGCTCGTGA CACTGGGAAG CCAAAACCAG TTTTCGATAA TTCTTCCAGA 
TCAGTTTCTG AGGATGAGAA GCCCAAAGGG ATGTCTTCTG ATTCTCCCAG GCCAGTCTTT 
GAGGATACCG GTCACAAGCC GGTCTCCTCT AATTCTCCCA AACCAGTTCC TGAGGAAGTG 
AAAGCGACCT ATTGGCCTCG ACCCCAGTTG GTCACAGGAT ACTGTACATA TCTTCCCTTT 
CGGGGATTTG ACATTACCCA GACTCCACCA GCCTTCATCA GTGTGTTACC GACTTTGCCC 
CAGTATAGCA TTTACACCCC TCTGGCCAGT GTTCCTACTG AGTATCACCT GCAAAGACCA 
ATACCAGTGG TGCCGTCTTT TGTAGCCAGT GACATAACGG AAGTGCATTT TGAGGCTCAT 
CATTTCAGTG CTGAGAATGC CCCTGGTACC CAGATTGCCT CCGAAACACA GATCCTTGAG 
GAATCTTTGG AAACACCAGT GAAGTCAGAG TGCAGCTATG ATGGTGCTGA TATAGTCCAG 
AGTGAGGCTA GCAGAAACGA TGACTACTGT GGCGATTCTG CCAAATGGGA AGTAAATCCA 
GAAAGCACCA ATGAAGTAAC AAATATTCCA TGTACACAAG TGGTTGCTGT ACAGGTGTCT 
TGGAATATAA CACATCAGGA AGTCAATACT GAGCCATATG GTCCTTTTGA GGCACAACAG 
GGGGGTATTT CACAGGTTGA AAAGGAGTAC CAGGTTTTAC AAGAGCAGCT TAAGGAAGCA 
TGTGAAAATT ATGAACACAG CAATGTCAAG GGCTCAGAAG AAACCAAGGA CTTGGAAGAA 
AAGTTGAAAA GGAATGTTGA AGAAAATAAG ATCTCAAAGA CAGAACTAGA TTGGTTCCTT 
GAAGATTTGA ACAAGGAAAT TAAAAAATGG GAACAGGAGA AAAAAGAAAT CCAAGAAAGA 
CTAAAGGCAC TGAAGAAGAA AATGAAAAAG GTGTTAAATG CCAGCAAAAT GTATACGCAG 
GAAAATGATG GAAAGGAAAA GGCACTTGAA TTACTTCTGG ATCAGTCCCT TGAAATCAGT 
AATACATTTA CAAATGAGAA AATGAAAATA GAAGAGAGTA TAAAGAAAGG GAAAGAGCAT 
TATGAAGAGA GTCTTCAAAG GGCTGTGGAT GCAGAGGTAT CTGTGCTTGA AAACTGGAAG 
GACATGGAAG TGTGTAAGTT ACAGAGCATG GAGTCACAAG CCGAAAGGTT TCTCAAGAAT 
CTGAAGCTAA TGAGCAGTGA TTCGGCAGCG TATTCTGACA TGGACTCAGA CATACATTCA 
TGGGAATCGT TTCTTTCTGA TGTTAGAGAA GAAATTGAGA AAGCAAAGTC TCAGTTTGAA 
GAACAAATTC AGGCAATTAA ATATGGTTCT CAGCTCAGTG AACTTCCTAA AGTGCAGATT 
TCTGAGCTTT CATTTCCTAC CTGTAACACG ATTCATCCTA AATTACTTCC TGAGTCTTCA 
GGCCAGGATG GTCAAGGTCC CTCCACTTCT ACAAGTCATG GGACCGGAGA CCAGACAGCA 
GCTCCCGAGG AGTCAAGTCT GGGCTCTGCA GCTGACAGCC CAGTGGAGTT GCCCTCCTGC 
AGTCAGAAAG CCCCCCAGCT GCCAGAGCAT GAGGGTGCTG GCCAGGCAAC TGGGCCAGAA 
CCAGGCCCAG TGGCTGATCA GAAGTTACCT GCTGCTCCAG AGCGTGCCAC TCATTCAAGC 
CAGTCTCCAA AGAAGCCGTT CAATAGCATT ATTGAGCACC TGTTGGTGGT GTTCCCGTGT 
TACAGCAGCA CGGAGCTTGC TGGTTTTATT AAAAAAGTTA GAAACAAAAA CAAGACCTCT 
CTGTCTGGAT TGAGTATTGA CGAAATTGTG CAAAGAGTGA CAGAACACGT CCTTGATGAA 
GAAAGAAAGA AAAAGCCAAG TCCAGGAAAG GGCTGGAGGC TGTCTGCACC CAGCTCTGCT 
GTTGCTGTGC CCAGGGCTGC CCAGGACCCG CCCTCCATGC CTGTCGGAGC ATCGCCCGAA 
ACCAGGGGGC CGAGGACCGA GGACGTCCCT GTGTCCTGCG CAAATTCCTG TGAAATGTGC 
CGAGAAGTAT TCAAGTCCAA ACACGTGCGT GAGCTGCAGT GTGGGCACAG GCTTCACAAG 
GGGTGCTTCA AGCAGTGGCT GAAGAGCCAG CGCACCTGCC CAGCCTGCCT GGGTCACGAC 
CTCCTGTCAG AGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005202115.1
CDS	124..6081
Translation	MADFPQGNFADYALLEDCPYVDDCIFAPEFMSSDYVRVTQLYCDGVGRQYKDYAQTEGNLEFDICTLWCSQPISVLQDYCDAIKVHIFWPLLFQRQQSSIITRMHSCVRTSGSHACEISLKKLQQLELMEDIVDLAKKVVNDTFFIGGLLRIGYKIENKILAMEEALNWVKYTGDVTILSKLGAVDNCWRMLSIFFTEYKYHITKVVTENCSLLEEFKTQSCAECLEQGELMKMKGNEEFSKERYDIAIIYYTRAIECRPENHLLYGNRALCFLRTGQFRNALGDGKRATILKNNWTKGHYRYCAALSMLGEYDWALQANIKAQKLCKNDPEGIKDLIQQHVKLQKQIEDLQGQTPASNPVKAFCESRAYIPSLSAPAFSTSLNFVETEKNFRKTNHEMANGGNQNPKVVDEALKVDDCDCHPEFVPPSSQPPKYKGKQKSRNNELEKFSPGSQETLPVDLKNILEKQFSKSSRAAHQDFANIMKMLRSLIQDGYTALLEQRCRSAAQAFTELLNGLDPQKIKQLNLAMINYVLVVYGLAISLLGIGQPEELSEAENQFKRMIEHYPNEGLDCLAYCGIGKVYLKKNRFLEALNHFEKARTLICRLPGVLTWPTSNVVIEETQPGKIKMLLEKFVEECRFPPVPDATCCYQKCHGYSKIQIYITDPDFKGFIRISCCQYCKIEFHMNCWKKLKTTTFNDKIDKDFLQGICLTPDCEGIISKIIIFSSGGQVKCEFEHKVIKEKVPPRPILKQKCSSLEKLRLKEDKKLKRKLQKKEAKKLAQERLEEDLRESNPPKTEEQRETINHVQSCQFLDDRILQCIKQYADKIKSGILNTSKLLKELLSWKVLTTEDYTTCFSSRNFLNEAVDYVIRHLIQEKNRVKTRVFLHVLSELKEVDPKLATWIQKLNSFGLDATGPFFSRYGAPLKELDFSIMTFLWNEKYGHKLGSIEGKQLDYFYEPTSLKEARCLIWLLEEHRDKFPALHNALDEFFDIMDSRCTVLRKQDSGDVPFNSTKIKNKGKKKKPKDSKPMLVGSGTPSVTPNNETVTSGEDHIKRSLSSAGPFVVPDHLRQDIEEFEALYEQHSNEYVVRNKKLWDINPKQKCSTLYDYFSQLLEEHGPLDMSNKMFSEEYEFFPEETQQILEKAGGLKAFLLGCPRFVVIDNCIALKKVALRLKKKRKKKNIKTKVEEISKTGEYLRVKLPLNPTAREFKPDVKSKPVSDLPSAPASEDMKPQPAPANSPQSARDTGKPKPVFDNSSRSVSEDEKPKGMSSDSPRPVFEDTGHKPVSSNSPKPVPEEVKATYWPRPQLVTGYCTYLPFRGFDITQTPPAFISVLPTLPQYSIYTPLASVPTEYHLQRPIPVVPSFVASDITEVHFEAHHFSAENAPGTQIASETQILEESLETPVKSECSYDGADIVQSEASRNDDYCGDSAKWEVNPESTNEVTNIPCTQVVAVQVSWNITHQEVNTEPYGPFEAQQGGISQVEKEYQVLQEQLKEACENYEHSNVKGSEETKDLEEKLKRNVEENKISKTELDWFLEDLNKEIKKWEQEKKEIQERLKALKKKMKKVLNASKMYTQENDGKEKALELLLDQSLEISNTFTNEKMKIEESIKKGKEHYEESLQRAVDAEVSVLENWKDMEVCKLQSMESQAERFLKNLKLMSSDSAAYSDMDSDIHSWESFLSDVREEIEKAKSQFEEQIQAIKYGSQLSELPKVQISELSFPTCNTIHPKLLPESSGQDGQGPSTSTSHGTGDQTAAPEESSLGSAADSPVELPSCSQKAPQLPEHEGAGQATGPEPGPVADQKLPAAPERATHSSQSPKKPFNSIIEHLLVVFPCYSSTELAGFIKKVRNKNKTSLSGLSIDEIVQRVTEHVLDEERKKKPSPGKGWRLSAPSSAVAVPRAAQDPPSMPVGASPETRGPRTEDVPVSCANSCEMCREVFKSKHVRELQCGHRLHKGCFKQWLKSQRTCPACLGHDLLSEE

Target ORF information:

RefSeq Version	XM_005202058.4
Organism	Bos taurus(cattle)
Definition	Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005202058.4

ORF Insert Sequence:

ATGGCCGATT TTCCCCAGGG AAATTTTGCA GATTATGCCT TGTTAGAAGA TTGCCCTTAT 
GTGGATGATT GTATCTTTGC CCCTGAGTTT ATGTCCAGTG ATTATGTTCG TGTGACTCAG 
CTTTACTGTG ATGGAGTGGG TAGACAGTAT AAAGATTATG CCCAAACTGA GGGAAACTTA 
GAATTTGACA TCTGCACTCT GTGGTGTAGT CAACCTATTT CTGTCCTGCA AGATTACTGT 
GATGCCATTA AAGTCCACAT CTTCTGGCCA CTTCTGTTTC AACGTCAGCA GAGCTCTATA 
ATAACACGGA TGCACTCCTG TGTAAGGACC AGCGGTTCTC ATGCTTGTGA GATAAGTTTG 
AAGAAATTAC AGCAGCTTGA ACTGATGGAA GATATTGTGG ATTTGGCAAA GAAAGTTGTG 
AATGATACAT TCTTTATCGG AGGCTTATTG AGAATTGGCT ATAAAATAGA GAATAAAATC 
TTGGCAATGG AAGAAGCTTT GAATTGGGTG AAATACACAG GCGATGTAAC AATTTTATCT 
AAATTAGGAG CAGTTGATAA TTGTTGGCGT ATGTTAAGTA TTTTCTTTAC TGAATACAAG 
TATCACATAA CTAAAGTTGT AACAGAAAAC TGCAGTTTGC TTGAAGAATT TAAAACTCAA 
AGTTGTGCGG AGTGTCTAGA GCAAGGAGAA CTAATGAAAA TGAAAGGAAA TGAAGAGTTT 
TCCAAAGAAA GATATGATAT AGCTATTATC TATTACACCA GAGCCATTGA ATGTAGGCCT 
GAAAATCACC TGCTTTATGG TAACCGAGCT CTTTGTTTTC TTCGTACTGG ACAATTCAGA 
AATGCACTTG GTGATGGAAA GAGAGCTACC ATTCTGAAGA ACAATTGGAC AAAGGGTCAT 
TATCGTTATT GTGCTGCTCT TTCTATGCTG GGGGAATATG ACTGGGCCCT GCAAGCAAAC 
ATAAAAGCTC AAAAACTCTG TAAAAATGAC CCTGAGGGAA TCAAGGATCT AATTCAGCAG 
CATGTAAAGT TACAAAAACA AATAGAAGAC CTGCAAGGTC AAACACCAGC TAGTAATCCA 
GTCAAAGCCT TCTGTGAAAG CAGGGCCTAC ATACCTAGTT TATCAGCACC TGCATTTAGT 
ACTTCACTTA ACTTTGTGGA AACTGAAAAA AATTTCAGAA AAACTAACCA CGAAATGGCA 
AACGGTGGTA ATCAGAATCC AAAGGTGGTA GATGAAGCTC TGAAAGTAGA TGATTGTGAC 
TGTCATCCTG AATTTGTACC GCCATCAAGT CAGCCTCCAA AATATAAAGG GAAACAAAAA 
TCTCGAAACA ATGAATTGGA GAAGTTCAGC CCTGGTTCAC AAGAAACTTT ACCAGTAGAT 
TTAAAAAACA TCTTGGAGAA ACAGTTTTCT AAATCTTCCA GAGCCGCACA CCAGGATTTT 
GCTAATATAA TGAAAATGTT GAGAAGTTTA ATTCAGGATG GCTACACGGC CTTGCTGGAG 
CAGCGCTGCC GCAGCGCCGC CCAAGCCTTC ACAGAGTTGC TCAACGGTTT AGATCCTCAA 
AAAATAAAGC AATTAAACCT GGCCATGATT AACTACGTGT TAGTAGTCTA CGGACTTGCC 
ATTTCACTCC TTGGAATAGG ACAGCCCGAG GAGTTATCTG AAGCTGAAAA CCAGTTCAAG 
AGGATGATTG AACACTACCC CAATGAGGGG CTTGATTGCT TGGCCTACTG TGGAATTGGA 
AAAGTGTATT TGAAAAAAAA CAGATTTTTA GAAGCTCTTA ATCATTTTGA GAAAGCAAGA 
ACCTTGATCT GTCGTCTTCC TGGAGTGTTA ACTTGGCCCA CAAGTAATGT GGTTATTGAG 
GAGACTCAGC CAGGGAAGAT AAAGATGCTG TTAGAGAAAT TTGTTGAAGA ATGCAGGTTC 
CCTCCAGTGC CGGATGCCAC TTGTTGCTAT CAGAAGTGCC ATGGATATTC CAAAATCCAG 
ATATATATAA CTGATCCAGA CTTTAAGGGT TTTATACGGA TCAGCTGTTG CCAGTATTGT 
AAAATAGAAT TTCACATGAA CTGCTGGAAG AAGTTAAAAA CAACAACCTT CAATGATAAA 
ATTGACAAGG ATTTTCTGCA AGGAATCTGT CTTACTCCTG ATTGTGAAGG AATCATTTCA 
AAGATTATCA TCTTCAGCAG TGGTGGTCAA GTTAAATGTG AATTTGAACA CAAGGTCATT 
AAAGAAAAGG TTCCTCCAAG ACCTATTCTG AAACAGAAAT GTTCTAGCCT AGAGAAGTTG 
AGGCTGAAAG AAGACAAAAA ATTGAAGCGA AAGCTCCAAA AAAAGGAAGC AAAAAAATTA 
GCACAAGAAA GACTGGAAGA GGACTTAAGG GAAAGTAACC CACCAAAAAC TGAAGAGCAG 
AGAGAAACTA TCAACCATGT CCAAAGTTGC CAGTTCCTTG ATGACAGAAT TCTGCAGTGC 
ATAAAGCAGT ACGCTGACAA GATTAAGTCC GGGATACTGA ATACTTCCAA GCTTCTCAAA 
GAATTGCTTT CTTGGAAGGT TTTAACCACA GAAGACTACA CAACGTGTTT TTCTAGCAGA 
AATTTCCTGA ATGAAGCAGT GGACTATGTC ATTCGTCATT TGATCCAAGA AAAGAATAGA 
GTAAAGACAA GGGTATTTCT GCATGTTTTG AGTGAGCTTA AAGAAGTGGA CCCAAAACTA 
GCCACCTGGA TCCAGAAACT GAATAGCTTT GGCTTAGATG CCACAGGACC TTTCTTTTCT 
CGTTATGGGG CTCCTCTTAA GGAGCTTGAT TTTAGCATCA TGACTTTTCT CTGGAATGAG 
AAATACGGTC ATAAGCTAGG CTCTATAGAA GGAAAGCAAC TTGATTATTT CTATGAGCCT 
ACATCACTGA AGGAAGCGCG GTGTTTAATA TGGCTGCTAG AAGAACACAG AGACAAGTTC 
CCAGCACTGC ATAATGCCTT GGACGAGTTC TTTGATATAA TGGACAGCCG CTGCACTGTA 
TTAAGGAAAC AGGACAGTGG TGACGTGCCA TTTAATTCTA CCAAGATAAA AAACAAAGGC 
AAGAAAAAGA AGCCAAAAGA TTCAAAGCCT ATGTTAGTTG GATCTGGAAC ACCTTCAGTC 
ACACCAAACA ATGAGACTGT CACTTCAGGT GAAGACCATA TCAAACGCAG TTTAAGTTCT 
GCGGGCCCCT TTGTAGTGCC TGATCACCTT CGGCAGGATA TAGAAGAATT TGAAGCTCTG 
TATGAACAGC ATAGTAATGA GTATGTTGTC CGTAATAAGA AGCTGTGGGA CATAAACCCA 
AAACAGAAAT GTTCAACTCT ATACGATTAT TTCTCTCAGT TGTTGGAGGA ACATGGTCCC 
TTGGACATGA GTAACAAAAT GTTTTCTGAA GAATATGAGT TCTTCCCAGA AGAAACTCAA 
CAGATACTAG AAAAAGCAGG AGGCTTGAAA GCGTTTCTCC TGGGATGTCC TCGTTTTGTT 
GTGATTGATA ACTGTATCGC ATTGAAGAAA GTTGCATTAC GGCTCAAGAA AAAGAGAAAG 
AAGAAAAACA TTAAAACAAA AGTAGAAGAA ATTTCAAAAA CAGGAGAGTA TTTACGAGTT 
AAACTACCAC TGAATCCAAC TGCTAGGGAA TTTAAACCAG ATGTAAAGTC CAAACCAGTG 
TCTGACTTAC CTTCAGCACC AGCTTCTGAG GACATGAAGC CTCAGCCTGC ACCTGCTAAT 
TCTCCCCAAT CAGCTCGTGA CACTGGGAAG CCAAAACCAG TTTTCGATAA TTCTTCCAGA 
TCAGTTTCTG AGGATGAGAA GCCCAAAGGG ATGTCTTCTG ATTCTCCCAG GCCAGTCTTT 
GAGGATACCG GTCACAAGCC GGTCTCCTCT AATTCTCCCA AACCAGTTCC TGAGGAAGTG 
AAAGCGACCT ATTGGCCTCG ACCCCAGTTG GTCACAGGAT ACTGTACATA TCTTCCCTTT 
CGGGGATTTG ACATTACCCA GACTCCACCA GCCTTCATCA GTGTGTTACC GACTTTGCCC 
CAGTATAGCA TTTACACCCC TCTGGCCAGT GTTCCTACTG AGTATCACCT GCAAAGACCA 
ATACCAGTGG TGCCGTCTTT TGTAGCCAGT GACATAACGG AAGTGCATTT TGAGGCTCAT 
CATTTCAGTG CTGAGAATGC CCCTGGTACC CAGATTGCCT CCGAAACACA GATCCTTGAG 
GAATCTTTGG AAACACCAGT GAAGTCAGAG TGCAGCTATG ATGGTGCTGA TATAGTCCAG 
AGTGAGGCTA GCAGAAACGA TGACTACTGT GGCGATTCTG CCAAATGGGA AGTAAATCCA 
GAAAGCACCA ATGAAGTAAC AAATATTCCA TGTACACAAG TGGTTGCTGT ACAGGTGTCT 
TGGAATATAA CACATCAGGA AGTCAATACT GAGCCATATG GTCCTTTTGA GGCACAACAG 
GGGGGTATTT CACAGGTTGA AAAGGAGTAC CAGGTTTTAC AAGAGCAGCT TAAGGAAGCA 
TGTGAAAATT ATGAACACAG CAATGTCAAG GGCTCAGAAG AAACCAAGGA CTTGGAAGAA 
AAGTTGAAAA GGAATGTTGA AGAAAATAAG ATCTCAAAGA CAGAACTAGA TTGGTTCCTT 
GAAGATTTGA ACAAGGAAAT TAAAAAATGG GAACAGGAGA AAAAAGAAAT CCAAGAAAGA 
CTAAAGGCAC TGAAGAAGAA AATGAAAAAG GTGTTAAATG CCAGCAAAAT GTATACGCAG 
GAAAATGATG GAAAGGAAAA GGCACTTGAA TTACTTCTGG ATCAGTCCCT TGAAATCAGT 
AATACATTTA CAAATGAGAA AATGAAAATA GAAGAGAGTA TAAAGAAAGG GAAAGAGCAT 
TATGAAGAGA GTCTTCAAAG GGCTGTGGAT GCAGAGGTAT CTGTGCTTGA AAACTGGAAG 
GACATGGAAG TGTGTAAGTT ACAGAGCATG GAGTCACAAG CCGAAAGGTT TCTCAAGAAT 
CTGAAGCTAA TGAGCAGTGA TTCGGCAGCG TATTCTGACA TGGACTCAGA CATACATTCA 
TGGGAATCGT TTCTTTCTGA TGTTAGAGAA GAAATTGAGA AAGCAAAGTC TCAGTTTGAA 
GAACAAATTC AGGCAATTAA ATATGGTTCT CAGCTCAGTG AACTTCCTAA AGTGCAGATT 
TCTGAGCTTT CATTTCCTAC CTGTAACACG ATTCATCCTA AATTACTTCC TGAGTCTTCA 
GGCCAGGATG GTCAAGGTCC CTCCACTTCT ACAAGTCATG GGACCGGAGA CCAGACAGCA 
GCTCCCGAGG AGTCAAGTCT GGGCTCTGCA GCTGACAGCC CAGTGGAGTT GCCCTCCTGC 
AGTCAGAAAG CCCCCCAGCT GCCAGAGCAT GAGGGTGCTG GCCAGGCAAC TGGGCCAGAA 
CCAGGCCCAG TGGCTGATCA GAAGTTACCT GCTGCTCCAG AGCGTGCCAC TCATTCAAGC 
CAGTCTCCAA AGAAGCCGTT CAATAGCATT ATTGAGCACC TGTTGGTGGT GTTCCCGTGT 
TACAGCAGCA CGGAGCTTGC TGGTTTTATT AAAAAAGTTA GAAACAAAAA CAAGACCTCT 
CTGTCTGGAT TGAGTATTGA CGAAATTGTG CAAAGAGTGA CAGAACACGT CCTTGATGAA 
GAAAGAAAGA AAAAGCCAAG TCCAGGAAAG GGCTGGAGGC TGTCTGCACC CAGCTCTGCT 
GTTGCTGTGC CCAGGGCTGC CCAGGACCCG CCCTCCATGC CTGTCGGAGC ATCGCCCGAA 
ACCAGGGGGC CGAGGACCGA GGACGTCCCT GTGTCCTGCG CAAATTCCTG TGAAATGTGC 
CGAGAAGTAT TCAAGTCCAA ACACGTGCGT GAGCTGCAGT GTGGGCACAG GCTTCACAAG 
GGGTGCTTCA AGCAGTGGCT GAAGAGCCAG CGCACCTGCC CAGCCTGCCT GGGTCACGAC 
CTCCTGTCAG AGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_010801674.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010799976.2 Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X5, mRNA.

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CloneID	OBa218654
Clone ID Related Accession (Same CDS sequence)	XM_010801674.2
Accession Version	XM_010801674.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5922bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TTC3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010801674.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACGAA AAAAAGCATT CTTGATGAAG GTGAAAAACA TCTGTGGGTG GTGTACCATG 
GCCGATTTTC CCCAGGGAAA TTTTGCAGAT TATGCCTTGT TAGAAGATTG CCCTTATGTG 
GATGATTGTA TCTTTGCCCC TGAGTTTATG TCCAGTGATT ATGTTCGTGT GACTCAGCTT 
TACTGTGATG GAGTGGGTAG ACAGTATAAA GATTATGCCC AAACTGAGGG AAACTTAGAA 
TTTGACATCT GCACTCTGTG GTGTAGTCAA CCTATTTCTG TCCTGCAAGA TTACTGTGAT 
GCCATTAAAG TCCACATCTT CTGGCCACTT CTGTTTCAAC GTCAGCAGAG CTCTATAATA 
ACACGGATGC ACTCCTGTGT AAGGACCAGC GGTTCTCATG CTTGTGAGAT AAGTTTGAAG 
AAATTACAGC AGCTTGAACT GATGGAAGAT ATTGTGGATT TGGCAAAGAA AGTTGTGAAT 
GATACATTCT TTATCGGAGG CTTATTGAGA ATTGGCTATA AAATAGAGAA TAAAATCTTG 
GCAATGGAAG AAGCTTTGAA TTGGGTGAAA TACACAGGCG ATGTAACAAT TTTATCTAAA 
TTAGGAGCAG TTGATAATTG TTGGCGTATG TTAAGTATTT TCTTTACTGA ATACAAGTAT 
CACATAACTA AAGTTGTAAC AGAAAACTGC AGTTTGCTTG AAGAATTTAA AACTCAAAGT 
TGTGCGGAGT GTCTAGAGCA AGGAGAACTA ATGAAAATGA AAGGAAATGA AGAGTTTTCC 
AAAGAAAGAT ATGATATAGC TATTATCTAT TACACCAGAG CCATTGAATG TAGGCCTGAA 
AATCACCTGC TTTATGGTAA CCGAGCTCTT TGTTTTCTTC GTACTGGACA ATTCAGAAAT 
GCACTTGGTG ATGGAAAGAG AGCTACCATT CTGAAGAACA ATTGGACAAA GGGTCATTAT 
CGTTATTGTG CTGCTCTTTC TATGCTGGGG GAATATGACT GGGCCCTGCA AGCAAACATA 
AAAGCTCAAA AACTCTGTAA AAATGACCCT GAGGGAATCA AGGATCTAAT TCAGCAGCAT 
GTAAAGTTAC AAAAACAAAT AGAAGACCTG CAAGGTCAAA CACCAGCTAG TAATCCAGTC 
AAAGCCTTCT GTGAAAGCAG GGCCTACATA CCTAGTTTAT CAGCACCTGC ATTTAGTACT 
TCACTTAACT TTGTGGAAAC TGAAAAAAAT TTCAGAAAAA CTAACCACGA AATGGCAAAC 
GGTGGTAATC AGAATCCAAA GGTGGTAGAT GAAGCTCTGA AAGTAGATGA TTGTGACTGT 
CATCCTGAAT TTGTACCGCC ATCAAGTCAG CCTCCAAAAT ATAAAGGGAA ACAAAAATCT 
CGAAACAATG AATTGGAGAA GTTCAGCCCT GGTTCACAAG AAACTTTACC AGTAGATTTA 
AAAAACATCT TGGAGAAACA GTTTTCTAAA TCTTCCAGAG CCGCACACCA GGATTTTGCT 
AATATAATGA AAATGTTGAG AAGTTTAATT CAGGATGGCT ACACGGCCTT GCTGGAGCAG 
CGCTGCCGCA GCGCCGCCCA AGCCTTCACA GAGTTGCTCA ACGGTTTAGA TCCTCAAAAA 
ATAAAGCAAT TAAACCTGGC CATGATTAAC TACGTGTTAG TAGTCTACGG ACTTGCCATT 
TCACTCCTTG GAATAGGACA GCCCGAGGAG TTATCTGAAG CTGAAAACCA GTTCAAGAGG 
ATGATTGAAC ACTACCCCAA TGAGGGGCTT GATTGCTTGG CCTACTGTGG AATTGGAAAA 
GTGTATTTGA AAAAAAACAG ATTTTTAGAA GCTCTTAATC ATTTTGAGAA AGCAAGAACC 
TTGATCTGTC GTCTTCCTGG AGTGTTAACT TGGCCCACAA GTAATGTGGT TATTGAGGAG 
ACTCAGCCAG GGAAGATAAA GATGCTGTTA GAGAAATTTG TTGAAGAATG CAGGTTCCCT 
CCAGTGCCGG ATGCCACTTG TTGCTATCAG AAGTGCCATG GATATTCCAA AATCCAGATA 
TATATAACTG ATCCAGACTT TAAGGGTTTT ATACGGATCA GCTGTTGCCA GTATTGTAAA 
ATAGAATTTC ACATGAACTG CTGGAAGAAG TTAAAAACAA CAACCTTCAA TGATAAAATT 
GACAAGGATT TTCTGCAAGG AATCTGTCTT ACTCCTGATT GTGAAGGAAT CATTTCAAAG 
ATTATCATCT TCAGCAGTGG TGGTCAAGTT AAATGTGAAT TTGAACACAA GGTCATTAAA 
GAAAAGGTTC CTCCAAGACC TATTCTGAAA CAGAAATGTT CTAGCCTAGA GAAGTTGAGG 
CTGAAAGAAG ACAAAAAATT GAAGCGAAAG CTCCAAAAAA AGGAAGCAAA AAAATTAGCA 
CAAGAAAGAC TGGAAGAGGA CTTAAGGGAA AGTAACCCAC CAAAAACTGA AGAGCAGAGA 
GAAACTATCA ACCATGTCCA AAGTTGCCAG TTCCTTGATG ACAGAATTCT GCAGTGCATA 
AAGCAGTACG CTGACAAGAT TAAGTCCGGG ATACTGAATA CTTCCAAGCT TCTCAAAGAA 
TTGCTTTCTT GGAAGGTTTT AACCACAGAA GACTACACAA CGTGTTTTTC TAGCAGAAAT 
TTCCTGAATG AAGCAGTGGA CTATGTCATT CGTCATTTGA TCCAAGAAAA GAATAGAGTA 
AAGACAAGGG TATTTCTGCA TGTTTTGAGT GAGCTTAAAG AAGTGGACCC AAAACTAGCC 
ACCTGGATCC AGAAACTGAA TAGCTTTGGC TTAGATGCCA CAGGACCTTT CTTTTCTCGT 
TATGGGGCTC CTCTTAAGGA GCTTGATTTT AGCATCATGA CTTTTCTCTG GAATGAGAAA 
TACGGTCATA AGCTAGGCTC TATAGAAGGA AAGCAACTTG ATTATTTCTA TGAGCCTACA 
TCACTGAAGG AAGCGCGGTG TTTAATATGG CTGCTAGAAG AACACAGAGA CAAGTTCCCA 
GCACTGCATA ATGCCTTGGA CGAGTTCTTT GATATAATGG ACAGCCGCTG CACTGTATTA 
AGGAAACAGG ACAGTGGTGA CGTGCCATTT AATTCTACCA AGATAAAAAA CAAAGGCAAG 
AAAAAGAAGC CAAAAGATTC AAAGCCTATG TTAGTTGGAT CTGGAACACC TTCAGTCACA 
CCAAACAATG AGACTGTCAC TTCAGGTGAA GACCATATCA AACGCAGTTT AAGTTCTGCG 
GGCCCCTTTG TAGTGCCTGA TCACCTTCGG CAGGATATAG AAGAATTTGA AGCTCTGTAT 
GAACAGCATA GTAATGAGTA TGTTGTCCGT AATAAGAAGC TGTGGGACAT AAACCCAAAA 
CAGAAATGTT CAACTCTATA CGATTATTTC TCTCAGTTGT TGGAGGAACA TGGTCCCTTG 
GACATGAGTA ACAAAATGTT TTCTGAAGAA TATGAGTTCT TCCCAGAAGA AACTCAACAG 
ATACTAGAAA AAGCAGGAGG CTTGAAAGCG TTTCTCCTGG GATGTCCTCG TTTTGTTGTG 
ATTGATAACT GTATCGCATT GAAGAAAGTT GCATTACGGC TCAAGAAAAA GAGAAAGAAG 
AAAAACATTA AAACAAAAGT AGAAGAAATT TCAAAAACAG GAGAGTATTT ACGAGTTAAA 
CTACCACTGA ATCCAACTGC TAGGGAATTT AAACCAGATG TAAAGTCCAA ACCAGTGTCT 
GACTTACCTT CAGCACCAGC TTCTGAGGAC ATGAAGCCTC AGCCTGCACC TGCTAATTCT 
CCCCAATCAG CTCGTGACAC TGGGAAGCCA AAACCAGTTT TCGATAATTC TTCCAGATCA 
GTTTCTGAGG ATGAGAAGCC CAAAGGGATG TCTTCTGATT CTCCCAGGCC AGTCTTTGAG 
GATACCGGTC ACAAGCCGGT CTCCTCTAAT TCTCCCAAAC CAGTTCCTGA GGAAGTGAAA 
GCGACCTATT GGCCTCGACC CCAGTTGGTC ACAGGATACT GTACATATCT TCCCTTTCGG 
GGATTTGACA TTACCCAGAC TCCACCAGCC TTCATCAGTG TGTTACCGAC TTTGCCCCAG 
TATAGCATTT ACACCCCTCT GGCCAGTGTT CCTACTGAGT ATCACCTGCA AAGACCAATA 
CCAGTGGTGC CGTCTTTTGT AGCCAGTGAC ATAACGGAAG TGCATTTTGA GGCTCATCAT 
TTCAGTGCTG AGAATGCCCC TGGTACCCAG ATTGCCTCCG AAACACAGAT CCTTGAGGAA 
TCTTTGGAAA CACCAGTGAA GTCAGAGTGC AGCTATGATG GTGCTGATAT AGTCCAGAGT 
GAGGCTAGCA GAAACGATGA CTACTGTGGC GATTCTGCCA AATGGGAAGT AAATCCAGAA 
AGCACCAATG AAGTAACAAA TATTCCATGT ACACAAGTGG TTGCTGTACA GGTGTCTTGG 
AATATAACAC ATCAGGAAGT CAATACTGAG CCATATGGTC CTTTTGAGGC ACAACAGGGG 
GGTATTTCAC AGGTTGAAAA GGAGTACCAG GTTTTACAAG AGCAGCTTAA GGAAGCATGT 
GAAAATTATG AACACAGCAA TGTCAAGGGC TCAGAAGAAA CCAAGGACTT GGAAGAAAAG 
TTGAAAAGGA ATGTTGAAGA AAATAAGATC TCAAAGACAG AACTAGATTG GTTCCTTGAA 
GATTTGAACA AGGAAATTAA AAAATGGGAA CAGGAGAAAA AAGAAATCCA AGAAAGACTA 
AAGGCACTGA AGAAGAAAAT GAAAAAGGTG TTAAATGCCA GCAAAATGTA TACGCAGGAA 
AATGATGGAA AGGAAAAGGC ACTTGAATTA CTTCTGGATC AGTCCCTTGA AATCAGTAAT 
ACATTTACAA ATGAGAAAAT GAAAATAGAA GAGAGTATAA AGAAAGGGAA AGAGCATTAT 
GAAGAGAGTC TTCAAAGGGC TGTGGATGCA GAGGTATCTG TGCTTGAAAA CTGGAAGGAC 
ATGGAAGTGT GTAAGTTACA GAGCATGGAG TCACAAGCCG AAAGGTTTCT CAAGAATCTG 
AAGCTAATGA GCAGTGATTC GGCAGCGTAT TCTGACATGG ACTCAGACAT ACATTCATGG 
GAATCGTTTC TTTCTGATGT TAGAGAAGAA ATTGAGAAAG CAAAGTCTCA GTTTGAAGAA 
CAAATTCAGG CAATTAAATA TGGTTCTCAG CTCAGTGAAC TTCCTAAAGT GCAGATTTCT 
GAGCTTTCAT TTCCTACCTG TAACACGATT CATCCTAAAT TACTTCCTGA GTCTTCAGGC 
CAGGATGGTC AAGGTCCCTC CACTTCTACA AGTCATGGGA CCGGAGACCA GACAGCAGCT 
CCCGAGGAGT CAAGTCTGGG CTCTGCAGCT GACAGCCCAG TGGAGTTGCC CTCCTGCAGT 
CAGAAAGCCC CCCAGCTGCC AGAGCATGAG GGTGCTGGCC AGGCAACTGG GCCAGAACCA 
GGCCCAGTGG CTGATCAGAA GTTACCTGCT GCTCCAGAGC GTGCCACTCA TTCAAGCCAG 
TCTCCAAAGA AGCCGTTCAA TAGCATTATT GAGCACCTGT TGGTGGTGTT CCCGTGTTAC 
AGCAGCACGG AGCTTGCTGG TTTTATTAAA AAAGTTAGAA ACAAAAACAA GACCTCTCTG 
TCTGGATTGA GTATTGACGA AATTGTGCAA AGAGTGACAG AACACGTCCT TGATGAAGAA 
AGAAAGAAAA AGCCAAGTCC AGGAAAGGGC TGGAGGCTGT CTGCACCCAG CTCTGCTGTT 
GCTGTGCCCA GGGCTGCCCA GGACCCGCCC TCCATGCCTG TCGGAGCATC GCCCGAAACC 
AGGGGGCCGA GGACCGAGGA CGTCCCTTGC TTCAAGCAGT GGCTGAAGAG CCAGCGCACC 
TGCCCAGCCT GCCTGGGTCA CGACCTCCTG TCAGAGGAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010799976.2
CDS	2833..8754
Translation	MGRKKAFLMKVKNICGWCTMADFPQGNFADYALLEDCPYVDDCIFAPEFMSSDYVRVTQLYCDGVGRQYKDYAQTEGNLEFDICTLWCSQPISVLQDYCDAIKVHIFWPLLFQRQQSSIITRMHSCVRTSGSHACEISLKKLQQLELMEDIVDLAKKVVNDTFFIGGLLRIGYKIENKILAMEEALNWVKYTGDVTILSKLGAVDNCWRMLSIFFTEYKYHITKVVTENCSLLEEFKTQSCAECLEQGELMKMKGNEEFSKERYDIAIIYYTRAIECRPENHLLYGNRALCFLRTGQFRNALGDGKRATILKNNWTKGHYRYCAALSMLGEYDWALQANIKAQKLCKNDPEGIKDLIQQHVKLQKQIEDLQGQTPASNPVKAFCESRAYIPSLSAPAFSTSLNFVETEKNFRKTNHEMANGGNQNPKVVDEALKVDDCDCHPEFVPPSSQPPKYKGKQKSRNNELEKFSPGSQETLPVDLKNILEKQFSKSSRAAHQDFANIMKMLRSLIQDGYTALLEQRCRSAAQAFTELLNGLDPQKIKQLNLAMINYVLVVYGLAISLLGIGQPEELSEAENQFKRMIEHYPNEGLDCLAYCGIGKVYLKKNRFLEALNHFEKARTLICRLPGVLTWPTSNVVIEETQPGKIKMLLEKFVEECRFPPVPDATCCYQKCHGYSKIQIYITDPDFKGFIRISCCQYCKIEFHMNCWKKLKTTTFNDKIDKDFLQGICLTPDCEGIISKIIIFSSGGQVKCEFEHKVIKEKVPPRPILKQKCSSLEKLRLKEDKKLKRKLQKKEAKKLAQERLEEDLRESNPPKTEEQRETINHVQSCQFLDDRILQCIKQYADKIKSGILNTSKLLKELLSWKVLTTEDYTTCFSSRNFLNEAVDYVIRHLIQEKNRVKTRVFLHVLSELKEVDPKLATWIQKLNSFGLDATGPFFSRYGAPLKELDFSIMTFLWNEKYGHKLGSIEGKQLDYFYEPTSLKEARCLIWLLEEHRDKFPALHNALDEFFDIMDSRCTVLRKQDSGDVPFNSTKIKNKGKKKKPKDSKPMLVGSGTPSVTPNNETVTSGEDHIKRSLSSAGPFVVPDHLRQDIEEFEALYEQHSNEYVVRNKKLWDINPKQKCSTLYDYFSQLLEEHGPLDMSNKMFSEEYEFFPEETQQILEKAGGLKAFLLGCPRFVVIDNCIALKKVALRLKKKRKKKNIKTKVEEISKTGEYLRVKLPLNPTAREFKPDVKSKPVSDLPSAPASEDMKPQPAPANSPQSARDTGKPKPVFDNSSRSVSEDEKPKGMSSDSPRPVFEDTGHKPVSSNSPKPVPEEVKATYWPRPQLVTGYCTYLPFRGFDITQTPPAFISVLPTLPQYSIYTPLASVPTEYHLQRPIPVVPSFVASDITEVHFEAHHFSAENAPGTQIASETQILEESLETPVKSECSYDGADIVQSEASRNDDYCGDSAKWEVNPESTNEVTNIPCTQVVAVQVSWNITHQEVNTEPYGPFEAQQGGISQVEKEYQVLQEQLKEACENYEHSNVKGSEETKDLEEKLKRNVEENKISKTELDWFLEDLNKEIKKWEQEKKEIQERLKALKKKMKKVLNASKMYTQENDGKEKALELLLDQSLEISNTFTNEKMKIEESIKKGKEHYEESLQRAVDAEVSVLENWKDMEVCKLQSMESQAERFLKNLKLMSSDSAAYSDMDSDIHSWESFLSDVREEIEKAKSQFEEQIQAIKYGSQLSELPKVQISELSFPTCNTIHPKLLPESSGQDGQGPSTSTSHGTGDQTAAPEESSLGSAADSPVELPSCSQKAPQLPEHEGAGQATGPEPGPVADQKLPAAPERATHSSQSPKKPFNSIIEHLLVVFPCYSSTELAGFIKKVRNKNKTSLSGLSIDEIVQRVTEHVLDEERKKKPSPGKGWRLSAPSSAVAVPRAAQDPPSMPVGASPETRGPRTEDVPCFKQWLKSQRTCPACLGHDLLSEE

Target ORF information:

RefSeq Version	XM_010801674.2
Organism	Bos taurus(cattle)
Definition	Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010801674.2

ORF Insert Sequence:

ATGGGACGAA AAAAAGCATT CTTGATGAAG GTGAAAAACA TCTGTGGGTG GTGTACCATG 
GCCGATTTTC CCCAGGGAAA TTTTGCAGAT TATGCCTTGT TAGAAGATTG CCCTTATGTG 
GATGATTGTA TCTTTGCCCC TGAGTTTATG TCCAGTGATT ATGTTCGTGT GACTCAGCTT 
TACTGTGATG GAGTGGGTAG ACAGTATAAA GATTATGCCC AAACTGAGGG AAACTTAGAA 
TTTGACATCT GCACTCTGTG GTGTAGTCAA CCTATTTCTG TCCTGCAAGA TTACTGTGAT 
GCCATTAAAG TCCACATCTT CTGGCCACTT CTGTTTCAAC GTCAGCAGAG CTCTATAATA 
ACACGGATGC ACTCCTGTGT AAGGACCAGC GGTTCTCATG CTTGTGAGAT AAGTTTGAAG 
AAATTACAGC AGCTTGAACT GATGGAAGAT ATTGTGGATT TGGCAAAGAA AGTTGTGAAT 
GATACATTCT TTATCGGAGG CTTATTGAGA ATTGGCTATA AAATAGAGAA TAAAATCTTG 
GCAATGGAAG AAGCTTTGAA TTGGGTGAAA TACACAGGCG ATGTAACAAT TTTATCTAAA 
TTAGGAGCAG TTGATAATTG TTGGCGTATG TTAAGTATTT TCTTTACTGA ATACAAGTAT 
CACATAACTA AAGTTGTAAC AGAAAACTGC AGTTTGCTTG AAGAATTTAA AACTCAAAGT 
TGTGCGGAGT GTCTAGAGCA AGGAGAACTA ATGAAAATGA AAGGAAATGA AGAGTTTTCC 
AAAGAAAGAT ATGATATAGC TATTATCTAT TACACCAGAG CCATTGAATG TAGGCCTGAA 
AATCACCTGC TTTATGGTAA CCGAGCTCTT TGTTTTCTTC GTACTGGACA ATTCAGAAAT 
GCACTTGGTG ATGGAAAGAG AGCTACCATT CTGAAGAACA ATTGGACAAA GGGTCATTAT 
CGTTATTGTG CTGCTCTTTC TATGCTGGGG GAATATGACT GGGCCCTGCA AGCAAACATA 
AAAGCTCAAA AACTCTGTAA AAATGACCCT GAGGGAATCA AGGATCTAAT TCAGCAGCAT 
GTAAAGTTAC AAAAACAAAT AGAAGACCTG CAAGGTCAAA CACCAGCTAG TAATCCAGTC 
AAAGCCTTCT GTGAAAGCAG GGCCTACATA CCTAGTTTAT CAGCACCTGC ATTTAGTACT 
TCACTTAACT TTGTGGAAAC TGAAAAAAAT TTCAGAAAAA CTAACCACGA AATGGCAAAC 
GGTGGTAATC AGAATCCAAA GGTGGTAGAT GAAGCTCTGA AAGTAGATGA TTGTGACTGT 
CATCCTGAAT TTGTACCGCC ATCAAGTCAG CCTCCAAAAT ATAAAGGGAA ACAAAAATCT 
CGAAACAATG AATTGGAGAA GTTCAGCCCT GGTTCACAAG AAACTTTACC AGTAGATTTA 
AAAAACATCT TGGAGAAACA GTTTTCTAAA TCTTCCAGAG CCGCACACCA GGATTTTGCT 
AATATAATGA AAATGTTGAG AAGTTTAATT CAGGATGGCT ACACGGCCTT GCTGGAGCAG 
CGCTGCCGCA GCGCCGCCCA AGCCTTCACA GAGTTGCTCA ACGGTTTAGA TCCTCAAAAA 
ATAAAGCAAT TAAACCTGGC CATGATTAAC TACGTGTTAG TAGTCTACGG ACTTGCCATT 
TCACTCCTTG GAATAGGACA GCCCGAGGAG TTATCTGAAG CTGAAAACCA GTTCAAGAGG 
ATGATTGAAC ACTACCCCAA TGAGGGGCTT GATTGCTTGG CCTACTGTGG AATTGGAAAA 
GTGTATTTGA AAAAAAACAG ATTTTTAGAA GCTCTTAATC ATTTTGAGAA AGCAAGAACC 
TTGATCTGTC GTCTTCCTGG AGTGTTAACT TGGCCCACAA GTAATGTGGT TATTGAGGAG 
ACTCAGCCAG GGAAGATAAA GATGCTGTTA GAGAAATTTG TTGAAGAATG CAGGTTCCCT 
CCAGTGCCGG ATGCCACTTG TTGCTATCAG AAGTGCCATG GATATTCCAA AATCCAGATA 
TATATAACTG ATCCAGACTT TAAGGGTTTT ATACGGATCA GCTGTTGCCA GTATTGTAAA 
ATAGAATTTC ACATGAACTG CTGGAAGAAG TTAAAAACAA CAACCTTCAA TGATAAAATT 
GACAAGGATT TTCTGCAAGG AATCTGTCTT ACTCCTGATT GTGAAGGAAT CATTTCAAAG 
ATTATCATCT TCAGCAGTGG TGGTCAAGTT AAATGTGAAT TTGAACACAA GGTCATTAAA 
GAAAAGGTTC CTCCAAGACC TATTCTGAAA CAGAAATGTT CTAGCCTAGA GAAGTTGAGG 
CTGAAAGAAG ACAAAAAATT GAAGCGAAAG CTCCAAAAAA AGGAAGCAAA AAAATTAGCA 
CAAGAAAGAC TGGAAGAGGA CTTAAGGGAA AGTAACCCAC CAAAAACTGA AGAGCAGAGA 
GAAACTATCA ACCATGTCCA AAGTTGCCAG TTCCTTGATG ACAGAATTCT GCAGTGCATA 
AAGCAGTACG CTGACAAGAT TAAGTCCGGG ATACTGAATA CTTCCAAGCT TCTCAAAGAA 
TTGCTTTCTT GGAAGGTTTT AACCACAGAA GACTACACAA CGTGTTTTTC TAGCAGAAAT 
TTCCTGAATG AAGCAGTGGA CTATGTCATT CGTCATTTGA TCCAAGAAAA GAATAGAGTA 
AAGACAAGGG TATTTCTGCA TGTTTTGAGT GAGCTTAAAG AAGTGGACCC AAAACTAGCC 
ACCTGGATCC AGAAACTGAA TAGCTTTGGC TTAGATGCCA CAGGACCTTT CTTTTCTCGT 
TATGGGGCTC CTCTTAAGGA GCTTGATTTT AGCATCATGA CTTTTCTCTG GAATGAGAAA 
TACGGTCATA AGCTAGGCTC TATAGAAGGA AAGCAACTTG ATTATTTCTA TGAGCCTACA 
TCACTGAAGG AAGCGCGGTG TTTAATATGG CTGCTAGAAG AACACAGAGA CAAGTTCCCA 
GCACTGCATA ATGCCTTGGA CGAGTTCTTT GATATAATGG ACAGCCGCTG CACTGTATTA 
AGGAAACAGG ACAGTGGTGA CGTGCCATTT AATTCTACCA AGATAAAAAA CAAAGGCAAG 
AAAAAGAAGC CAAAAGATTC AAAGCCTATG TTAGTTGGAT CTGGAACACC TTCAGTCACA 
CCAAACAATG AGACTGTCAC TTCAGGTGAA GACCATATCA AACGCAGTTT AAGTTCTGCG 
GGCCCCTTTG TAGTGCCTGA TCACCTTCGG CAGGATATAG AAGAATTTGA AGCTCTGTAT 
GAACAGCATA GTAATGAGTA TGTTGTCCGT AATAAGAAGC TGTGGGACAT AAACCCAAAA 
CAGAAATGTT CAACTCTATA CGATTATTTC TCTCAGTTGT TGGAGGAACA TGGTCCCTTG 
GACATGAGTA ACAAAATGTT TTCTGAAGAA TATGAGTTCT TCCCAGAAGA AACTCAACAG 
ATACTAGAAA AAGCAGGAGG CTTGAAAGCG TTTCTCCTGG GATGTCCTCG TTTTGTTGTG 
ATTGATAACT GTATCGCATT GAAGAAAGTT GCATTACGGC TCAAGAAAAA GAGAAAGAAG 
AAAAACATTA AAACAAAAGT AGAAGAAATT TCAAAAACAG GAGAGTATTT ACGAGTTAAA 
CTACCACTGA ATCCAACTGC TAGGGAATTT AAACCAGATG TAAAGTCCAA ACCAGTGTCT 
GACTTACCTT CAGCACCAGC TTCTGAGGAC ATGAAGCCTC AGCCTGCACC TGCTAATTCT 
CCCCAATCAG CTCGTGACAC TGGGAAGCCA AAACCAGTTT TCGATAATTC TTCCAGATCA 
GTTTCTGAGG ATGAGAAGCC CAAAGGGATG TCTTCTGATT CTCCCAGGCC AGTCTTTGAG 
GATACCGGTC ACAAGCCGGT CTCCTCTAAT TCTCCCAAAC CAGTTCCTGA GGAAGTGAAA 
GCGACCTATT GGCCTCGACC CCAGTTGGTC ACAGGATACT GTACATATCT TCCCTTTCGG 
GGATTTGACA TTACCCAGAC TCCACCAGCC TTCATCAGTG TGTTACCGAC TTTGCCCCAG 
TATAGCATTT ACACCCCTCT GGCCAGTGTT CCTACTGAGT ATCACCTGCA AAGACCAATA 
CCAGTGGTGC CGTCTTTTGT AGCCAGTGAC ATAACGGAAG TGCATTTTGA GGCTCATCAT 
TTCAGTGCTG AGAATGCCCC TGGTACCCAG ATTGCCTCCG AAACACAGAT CCTTGAGGAA 
TCTTTGGAAA CACCAGTGAA GTCAGAGTGC AGCTATGATG GTGCTGATAT AGTCCAGAGT 
GAGGCTAGCA GAAACGATGA CTACTGTGGC GATTCTGCCA AATGGGAAGT AAATCCAGAA 
AGCACCAATG AAGTAACAAA TATTCCATGT ACACAAGTGG TTGCTGTACA GGTGTCTTGG 
AATATAACAC ATCAGGAAGT CAATACTGAG CCATATGGTC CTTTTGAGGC ACAACAGGGG 
GGTATTTCAC AGGTTGAAAA GGAGTACCAG GTTTTACAAG AGCAGCTTAA GGAAGCATGT 
GAAAATTATG AACACAGCAA TGTCAAGGGC TCAGAAGAAA CCAAGGACTT GGAAGAAAAG 
TTGAAAAGGA ATGTTGAAGA AAATAAGATC TCAAAGACAG AACTAGATTG GTTCCTTGAA 
GATTTGAACA AGGAAATTAA AAAATGGGAA CAGGAGAAAA AAGAAATCCA AGAAAGACTA 
AAGGCACTGA AGAAGAAAAT GAAAAAGGTG TTAAATGCCA GCAAAATGTA TACGCAGGAA 
AATGATGGAA AGGAAAAGGC ACTTGAATTA CTTCTGGATC AGTCCCTTGA AATCAGTAAT 
ACATTTACAA ATGAGAAAAT GAAAATAGAA GAGAGTATAA AGAAAGGGAA AGAGCATTAT 
GAAGAGAGTC TTCAAAGGGC TGTGGATGCA GAGGTATCTG TGCTTGAAAA CTGGAAGGAC 
ATGGAAGTGT GTAAGTTACA GAGCATGGAG TCACAAGCCG AAAGGTTTCT CAAGAATCTG 
AAGCTAATGA GCAGTGATTC GGCAGCGTAT TCTGACATGG ACTCAGACAT ACATTCATGG 
GAATCGTTTC TTTCTGATGT TAGAGAAGAA ATTGAGAAAG CAAAGTCTCA GTTTGAAGAA 
CAAATTCAGG CAATTAAATA TGGTTCTCAG CTCAGTGAAC TTCCTAAAGT GCAGATTTCT 
GAGCTTTCAT TTCCTACCTG TAACACGATT CATCCTAAAT TACTTCCTGA GTCTTCAGGC 
CAGGATGGTC AAGGTCCCTC CACTTCTACA AGTCATGGGA CCGGAGACCA GACAGCAGCT 
CCCGAGGAGT CAAGTCTGGG CTCTGCAGCT GACAGCCCAG TGGAGTTGCC CTCCTGCAGT 
CAGAAAGCCC CCCAGCTGCC AGAGCATGAG GGTGCTGGCC AGGCAACTGG GCCAGAACCA 
GGCCCAGTGG CTGATCAGAA GTTACCTGCT GCTCCAGAGC GTGCCACTCA TTCAAGCCAG 
TCTCCAAAGA AGCCGTTCAA TAGCATTATT GAGCACCTGT TGGTGGTGTT CCCGTGTTAC 
AGCAGCACGG AGCTTGCTGG TTTTATTAAA AAAGTTAGAA ACAAAAACAA GACCTCTCTG 
TCTGGATTGA GTATTGACGA AATTGTGCAA AGAGTGACAG AACACGTCCT TGATGAAGAA 
AGAAAGAAAA AGCCAAGTCC AGGAAAGGGC TGGAGGCTGT CTGCACCCAG CTCTGCTGTT 
GCTGTGCCCA GGGCTGCCCA GGACCCGCCC TCCATGCCTG TCGGAGCATC GCCCGAAACC 
AGGGGGCCGA GGACCGAGGA CGTCCCTTGC TTCAAGCAGT GGCTGAAGAG CCAGCGCACC 
TGCCCAGCCT GCCTGGGTCA CGACCTCCTG TCAGAGGAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_010801675.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010799977.2 Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X6, mRNA.

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CloneID	OBa218655
Clone ID Related Accession (Same CDS sequence)	XM_010801675.2
Accession Version	XM_010801675.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5880bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TTC3 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010801675.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACGAA AAAAAGCATT CTTGATGAAG GTGAAAAACA TCTGTGGGTG GTGTACCATG 
GCCGATTTTC CCCAGGGAAA TTTTGCAGAT TATGCCTTGT TAGAAGATTG CCCTTATGTG 
GATGATTGTA TCTTTGCCCC TGAGTTTATG TCCAGTGATT ATGTTCGTGT GACTCAGCTT 
TACTGTGATG GAGTGGGTAG ACAGTATAAA GATTATGCCC AAACTGAGGG AAACTTAGAA 
TTTGACATCT GCACTCTGTG GTGTAGTCAA CCTATTTCTG TCCTGCAAGA TTACTGTGAT 
GCCATTAAAG TCCACATCTT CTGGCCACTT CTGTTTCAAC GTCAGCAGAG CTCTATAATA 
ACACGGATGC ACTCCTGTGT AAGGACCAGC GGTTCTCATG CTTGTGAGAT AAGTTTGAAG 
AAATTACAGC AGCTTGAACT GATGGAAGAT ATTGTGGATT TGGCAAAGAA AGTTGTGAAT 
GATACATTCT TTATCGGAGG CTTATTGAGA ATTGGCTATA AAATAGAGAA TAAAATCTTG 
GCAATGGAAG AAGCTTTGAA TTGGGTGAAA TACACAGGCG ATGTAACAAT TTTATCTAAA 
TTAGGAGCAG TTGATAATTG TTGGCGTATG TTAAGTATTT TCTTTACTGA ATACAAGTAT 
CACATAACTA AAGTTGTAAC AGAAAACTGC AGTTTGCTTG AAGAATTTAA AACTCAAAGT 
TGTGCGGAGT GTCTAGAGCA AGGAGAACTA ATGAAAATGA AAGGAAATGA AGAGTTTTCC 
AAAGAAAGAT ATGATATAGC TATTATCTAT TACACCAGAG CCATTGAATG TAGGCCTGAA 
AATCACCTGC TTTATGGTAA CCGAGCTCTT TGTTTTCTTC GTACTGGACA ATTCAGAAAT 
GCACTTGGTG ATGGAAAGAG AGCTACCATT CTGAAGAACA ATTGGACAAA GGGTCATTAT 
CGTTATTGTG CTGCTCTTTC TATGCTGGGG GAATATGACT GGGCCCTGCA AGCAAACATA 
AAAGCTCAAA AACTCTGTAA AAATGACCCT GAGGGAATCA AGGATCTAAT TCAGCAGCAT 
GTAAAGTTAC AAAAACAAAT AGAAGACCTG CAAGGTCAAA CACCAGCTAG TAATCCAGTC 
AAAGCCTTCT GTGAAAGCAG GGCCTACATA CCTAGTTTAT CAGCACCTGC ATTTAGTACT 
TCACTTAACT TTGTGGAAAC TGAAAAAAAT TTCAGAAAAA CTAACCACGA AATGGCAAAC 
GGTGGTAATC AGAATCCAAA GGTGGTAGAT GAAGCTCTGA AAGTAGATGA TTGTGACTGT 
CATCCTGAAT TTGTACCGCC ATCAAGTCAG CCTCCAAAAT ATAAAGGGAA ACAAAAATCT 
CGAAACAATG AATTGGAGAA GTTCAGCCCT GGTTCACAAG AAACTTTACC AGTAGATTTA 
AAAAACATCT TGGAGAAACA GTTTTCTAAA TCTTCCAGAG CCGCACACCA GGATTTTGCT 
AATATAATGA AAATGTTGAG AAGTTTAATT CAGGATGGCT ACACGGCCTT GCTGGAGCAG 
CGCTGCCGCA GCGCCGCCCA AGCCTTCACA GAGTTGCTCA ACGGTTTAGA TCCTCAAAAA 
ATAAAGCAAT TAAACCTGGC CATGATTAAC TACGTGTTAG TAGTCTACGG ACTTGCCATT 
TCACTCCTTG GAATAGGACA GCCCGAGGAG TTATCTGAAG CTGAAAACCA GTTCAAGAGG 
ATGATTGAAC ACTACCCCAA TGAGGGGCTT GATTGCTTGG CCTACTGTGG AATTGGAAAA 
GTGTATTTGA AAAAAAACAG ATTTTTAGAA GCTCTTAATC ATTTTGAGAA AGCAAGAACC 
TTGATCTGTC GTCTTCCTGG AGTGTTAACT TGGCCCACAA GTAATGTGGT TATTGAGGAG 
ACTCAGCCAG GGAAGATAAA GATGCTGTTA GAGAAATTTG TTGAAGAATG CAGGTTCCCT 
CCAGTGCCGG ATGCCACTTG TTGCTATCAG AAGTGCCATG GATATTCCAA AATCCAGATA 
TATATAACTG ATCCAGACTT TAAGGGTTTT ATACGGATCA GCTGTTGCCA GTATTGTAAA 
ATAGAATTTC ACATGAACTG CTGGAAGAAG TTAAAAACAA CAACCTTCAA TGATAAAATT 
GACAAGGATT TTCTGCAAGG AATCTGTCTT ACTCCTGATT GTGAAGGAAT CATTTCAAAG 
ATTATCATCT TCAGCAGTGG TGGTCAAGTT AAATGTGAAT TTGAACACAA GGTCATTAAA 
GAAAAGGTTC CTCCAAGACC TATTCTGAAA CAGAAATGTT CTAGCCTAGA GAAGTTGAGG 
CTGAAAGAAG ACAAAAAATT GAAGCGAAAG CTCCAAAAAA AGGAAGCAAA AAAATTAGCA 
CAAGAAAGAC TGGAAGAGGA CTTAAGGGAA AGTAACCCAC CAAAAACTGA AGAGCAGAGA 
GAAACTATCA ACCATGTCCA AAGTTGCCAG TTCCTTGATG ACAGAATTCT GCAGTGCATA 
AAGCAGTACG CTGACAAGAT TAAGTCCGGG ATACTGAATA CTTCCAAGCT TCTCAAAGAA 
TTGCTTTCTT GGAAGGTTTT AACCACAGAA GACTACACAA CGTGTTTTTC TAGCAGAAAT 
TTCCTGAATG AAGCAGTGGA CTATGTCATT CGTCATTTGA TCCAAGAAAA GAATAGAGTA 
AAGACAAGGG TATTTCTGCA TGTTTTGAGT GAGCTTAAAG AAGTGGACCC AAAACTAGCC 
ACCTGGATCC AGAAACTGAA TAGCTTTGGC TTAGATGCCA CAGGACCTTT CTTTTCTCGT 
TATGGGGCTC CTCTTAAGGA GCTTGATTTT AGCATCATGA CTTTTCTCTG GAATGAGAAA 
TACGGTCATA AGCTAGGCTC TATAGAAGGA AAGCAACTTG ATTATTTCTA TGAGCCTACA 
TCACTGAAGG AAGCGCGGTG TTTAATATGG CTGCTAGAAG AACACAGAGA CAAGTTCCCA 
GCACTGCATA ATGCCTTGGA CGAGTTCTTT GATATAATGG ACAGCCGCTG CACTGTATTA 
AGGAAACAGG ACAGTGGTGA CGTGCCATTT AATTCTACCA AGATAAAAAA CAAAGGCAAG 
AAAAAGAAGC CAAAAGATTC AAAGCCTATG TTAGTTGGAT CTGGAACACC TTCAGTCACA 
CCAAACAATG AGACTGTCAC TTCAGGTGAA GACCATATCA AACGCAGTTT AAGTTCTGCG 
GGCCCCTTTG TAGTGCCTGA TCACCTTCGG CAGGATATAG AAGAATTTGA AGCTCTGTAT 
GAACAGCATA GTAATGAGTA TGTTGTCCGT AATAAGAAGC TGTGGGACAT AAACCCAAAA 
CAGAAATGTT CAACTCTATA CGATTATTTC TCTCAGTTGT TGGAGGAACA TGGTCCCTTG 
GACATGAGTA ACAAAATGTT TTCTGAAGAA TATGAGTTCT TCCCAGAAGA AACTCAACAG 
ATACTAGAAA AAGCAGGAGG CTTGAAAGCG TTTCTCCTGG GATGTCCTCG TTTTGTTGTG 
ATTGATAACT GTATCGCATT GAAGAAAGTT GCATTACGGC TCAAGAAAAA GAGAAAGAAG 
AAAAACATTA AAACAAAAGT AGAAGAAATT TCAAAAACAG GAGAGTATTT ACGAGTTAAA 
CTACCACTGA ATCCAACTGC TAGGGAATTT AAACCAGATG TAAAGTCCAA ACCAGTGTCT 
GACTTACCTT CAGCACCAGC TTCTGAGGAC ATGAAGCCTC AGCCTGCACC TGCTAATTCT 
CCCCAATCAG CTCGTGACAC TGGGAAGCCA AAACCAGTTT TCGATAATTC TTCCAGATCA 
GTTTCTGAGG ATGAGAAGCC CAAAGGGATG TCTTCTGATT CTCCCAGGCC AGTCTTTGAG 
GATACCGGTC ACAAGCCGGT CTCCTCTAAT TCTCCCAAAC CAGTTCCTGA GGAAGTGAAA 
GCGACCTATT GGCCTCGACC CCAGTTGGTC ACAGGATACT GTACATATCT TCCCTTTCGG 
GGATTTGACA TTACCCAGAC TCCACCAGCC TTCATCAGTG TGTTACCGAC TTTGCCCCAG 
TATAGCATTT ACACCCCTCT GGCCAGTGTT CCTACTGAGT ATCACCTGCA AAGACCAATA 
CCAGTGGTGC CGTCTTTTGT AGCCAGTGAC ATAACGGAAG TGCATTTTGA GGCTCATCAT 
TTCAGTGCTG AGAATGCCCC TGGTACCCAG ATTGCCTCCG AAACACAGAT CCTTGAGGAA 
TCTTTGGAAA CACCAGTGAA GTCAGAGTGC AGCTATGATG GTGCTGATAT AGTCCAGAGT 
GAGGCTAGCA GAAACGATGA CTACTGTGGC GATTCTGCCA AATGGGAAGT AAATCCAGAA 
AGCACCAATG AAGTAACAAA TATTCCATGT ACACAAGTGG TTGCTGTACA GGTGTCTTGG 
AATATAACAC ATCAGGAAGT CAATACTGAG CCATATGGTC CTTTTGAGGC ACAACAGGGG 
GGTATTTCAC AGGTTGAAAA GGAGTACCAG GTTTTACAAG AGCAGCTTAA GGAAGCATGT 
GAAAATTATG AACACAGCAA TGTCAAGGGC TCAGAAGAAA CCAAGGACTT GGAAGAAAAG 
TTGAAAAGGA ATGTTGAAGA AAATAAGATC TCAAAGACAG AACTAGATTG GTTCCTTGAA 
GATTTGAACA AGGAAATTAA AAAATGGGAA CAGGAGAAAA AAGAAATCCA AGAAAGACTA 
AAGGCACTGA AGAAGAAAAT GAAAAAGGTG TTAAATGCCA GCAAAATGTA TACGCAGGAA 
AATGATGGAA AGGAAAAGGC ACTTGAATTA CTTCTGGATC AGTCCCTTGA AATCAGTAAT 
ACATTTACAA ATGAGAAAAT GAAAATAGAA GAGAGTATAA AGAAAGGGAA AGAGCATTAT 
GAAGAGAGTC TTCAAAGGGC TGTGGATGCA GAGGTATCTG TGCTTGAAAA CTGGAAGGAC 
ATGGAAGTGT GTAAGTTACA GAGCATGGAG TCACAAGCCG AAAGGTTTCT CAAGAATCTG 
AAGCTAATGA GCAGTGATTC GGCAGCGTAT TCTGACATGG ACTCAGACAT ACATTCATGG 
GAATCGTTTC TTTCTGATGT TAGAGAAGAA ATTGAGAAAG CAAAGTCTCA GTTTGAAGAA 
CAAATTCAGG CAATTAAATA TGGTTCTCAG CTCAGTGAAC TTCCTAAAGT GCAGATTTCT 
GAGCTTTCAT TTCCTACCTG TAACACGATT CATCCTAAAT TACTTCCTGA GTCTTCAGGC 
CAGGATGGTC AAGGTCCCTC CACTTCTACA AGTCATGGGA CCGGAGACCA GACAGCAGCT 
CCCGAGGAGT CAAGTCTGGG CTCTGCAGCT GACAGCCCAG TGGAGTTGCC CTCCTGCAGT 
CAGAAAGCCC CCCAGCTGCC AGAGCATGAG GGTGCTGGCC AGGCAACTGG GCCAGAACCA 
GGCCCAGTGG CTGATCAGAA GTTACCTGCT GCTCCAGAGC GTGCCACTCA TTCAAGCCAG 
TCTCCAAAGA AGCCGTTCAA TAGCATTATT GAGCACCTGT TGGTGGTGTT CCCGTGTTAC 
AGCAGCACGG AGCTTGCTGG TTTTATTAAA AAAGTTAGAA ACAAAAACAA GACCTCTCTG 
TCTGGATTGA GTATTGACGA AATTGTGCAA AGAGTGACAG AACACGTCCT TGATGAAGAA 
AGAAAGAAAA AGGTGTCCTG CGCAAATTCC TGTGAAATGT GCCGAGAAGT ATTCAAGTCC 
AAACACGTGC GTGAGCTGCA GTGTGGGCAC AGGCTTCACA AGGGGTGCTT CAAGCAGTGG 
CTGAAGAGCC AGCGCACCTG CCCAGCCTGC CTGGGTCACG ACCTCCTGTC AGAGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010799977.2
CDS	2832..8711
Translation	MGRKKAFLMKVKNICGWCTMADFPQGNFADYALLEDCPYVDDCIFAPEFMSSDYVRVTQLYCDGVGRQYKDYAQTEGNLEFDICTLWCSQPISVLQDYCDAIKVHIFWPLLFQRQQSSIITRMHSCVRTSGSHACEISLKKLQQLELMEDIVDLAKKVVNDTFFIGGLLRIGYKIENKILAMEEALNWVKYTGDVTILSKLGAVDNCWRMLSIFFTEYKYHITKVVTENCSLLEEFKTQSCAECLEQGELMKMKGNEEFSKERYDIAIIYYTRAIECRPENHLLYGNRALCFLRTGQFRNALGDGKRATILKNNWTKGHYRYCAALSMLGEYDWALQANIKAQKLCKNDPEGIKDLIQQHVKLQKQIEDLQGQTPASNPVKAFCESRAYIPSLSAPAFSTSLNFVETEKNFRKTNHEMANGGNQNPKVVDEALKVDDCDCHPEFVPPSSQPPKYKGKQKSRNNELEKFSPGSQETLPVDLKNILEKQFSKSSRAAHQDFANIMKMLRSLIQDGYTALLEQRCRSAAQAFTELLNGLDPQKIKQLNLAMINYVLVVYGLAISLLGIGQPEELSEAENQFKRMIEHYPNEGLDCLAYCGIGKVYLKKNRFLEALNHFEKARTLICRLPGVLTWPTSNVVIEETQPGKIKMLLEKFVEECRFPPVPDATCCYQKCHGYSKIQIYITDPDFKGFIRISCCQYCKIEFHMNCWKKLKTTTFNDKIDKDFLQGICLTPDCEGIISKIIIFSSGGQVKCEFEHKVIKEKVPPRPILKQKCSSLEKLRLKEDKKLKRKLQKKEAKKLAQERLEEDLRESNPPKTEEQRETINHVQSCQFLDDRILQCIKQYADKIKSGILNTSKLLKELLSWKVLTTEDYTTCFSSRNFLNEAVDYVIRHLIQEKNRVKTRVFLHVLSELKEVDPKLATWIQKLNSFGLDATGPFFSRYGAPLKELDFSIMTFLWNEKYGHKLGSIEGKQLDYFYEPTSLKEARCLIWLLEEHRDKFPALHNALDEFFDIMDSRCTVLRKQDSGDVPFNSTKIKNKGKKKKPKDSKPMLVGSGTPSVTPNNETVTSGEDHIKRSLSSAGPFVVPDHLRQDIEEFEALYEQHSNEYVVRNKKLWDINPKQKCSTLYDYFSQLLEEHGPLDMSNKMFSEEYEFFPEETQQILEKAGGLKAFLLGCPRFVVIDNCIALKKVALRLKKKRKKKNIKTKVEEISKTGEYLRVKLPLNPTAREFKPDVKSKPVSDLPSAPASEDMKPQPAPANSPQSARDTGKPKPVFDNSSRSVSEDEKPKGMSSDSPRPVFEDTGHKPVSSNSPKPVPEEVKATYWPRPQLVTGYCTYLPFRGFDITQTPPAFISVLPTLPQYSIYTPLASVPTEYHLQRPIPVVPSFVASDITEVHFEAHHFSAENAPGTQIASETQILEESLETPVKSECSYDGADIVQSEASRNDDYCGDSAKWEVNPESTNEVTNIPCTQVVAVQVSWNITHQEVNTEPYGPFEAQQGGISQVEKEYQVLQEQLKEACENYEHSNVKGSEETKDLEEKLKRNVEENKISKTELDWFLEDLNKEIKKWEQEKKEIQERLKALKKKMKKVLNASKMYTQENDGKEKALELLLDQSLEISNTFTNEKMKIEESIKKGKEHYEESLQRAVDAEVSVLENWKDMEVCKLQSMESQAERFLKNLKLMSSDSAAYSDMDSDIHSWESFLSDVREEIEKAKSQFEEQIQAIKYGSQLSELPKVQISELSFPTCNTIHPKLLPESSGQDGQGPSTSTSHGTGDQTAAPEESSLGSAADSPVELPSCSQKAPQLPEHEGAGQATGPEPGPVADQKLPAAPERATHSSQSPKKPFNSIIEHLLVVFPCYSSTELAGFIKKVRNKNKTSLSGLSIDEIVQRVTEHVLDEERKKKVSCANSCEMCREVFKSKHVRELQCGHRLHKGCFKQWLKSQRTCPACLGHDLLSEE

Target ORF information:

RefSeq Version	XM_010801675.2
Organism	Bos taurus(cattle)
Definition	Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010801675.2

ORF Insert Sequence:

ATGGGACGAA AAAAAGCATT CTTGATGAAG GTGAAAAACA TCTGTGGGTG GTGTACCATG 
GCCGATTTTC CCCAGGGAAA TTTTGCAGAT TATGCCTTGT TAGAAGATTG CCCTTATGTG 
GATGATTGTA TCTTTGCCCC TGAGTTTATG TCCAGTGATT ATGTTCGTGT GACTCAGCTT 
TACTGTGATG GAGTGGGTAG ACAGTATAAA GATTATGCCC AAACTGAGGG AAACTTAGAA 
TTTGACATCT GCACTCTGTG GTGTAGTCAA CCTATTTCTG TCCTGCAAGA TTACTGTGAT 
GCCATTAAAG TCCACATCTT CTGGCCACTT CTGTTTCAAC GTCAGCAGAG CTCTATAATA 
ACACGGATGC ACTCCTGTGT AAGGACCAGC GGTTCTCATG CTTGTGAGAT AAGTTTGAAG 
AAATTACAGC AGCTTGAACT GATGGAAGAT ATTGTGGATT TGGCAAAGAA AGTTGTGAAT 
GATACATTCT TTATCGGAGG CTTATTGAGA ATTGGCTATA AAATAGAGAA TAAAATCTTG 
GCAATGGAAG AAGCTTTGAA TTGGGTGAAA TACACAGGCG ATGTAACAAT TTTATCTAAA 
TTAGGAGCAG TTGATAATTG TTGGCGTATG TTAAGTATTT TCTTTACTGA ATACAAGTAT 
CACATAACTA AAGTTGTAAC AGAAAACTGC AGTTTGCTTG AAGAATTTAA AACTCAAAGT 
TGTGCGGAGT GTCTAGAGCA AGGAGAACTA ATGAAAATGA AAGGAAATGA AGAGTTTTCC 
AAAGAAAGAT ATGATATAGC TATTATCTAT TACACCAGAG CCATTGAATG TAGGCCTGAA 
AATCACCTGC TTTATGGTAA CCGAGCTCTT TGTTTTCTTC GTACTGGACA ATTCAGAAAT 
GCACTTGGTG ATGGAAAGAG AGCTACCATT CTGAAGAACA ATTGGACAAA GGGTCATTAT 
CGTTATTGTG CTGCTCTTTC TATGCTGGGG GAATATGACT GGGCCCTGCA AGCAAACATA 
AAAGCTCAAA AACTCTGTAA AAATGACCCT GAGGGAATCA AGGATCTAAT TCAGCAGCAT 
GTAAAGTTAC AAAAACAAAT AGAAGACCTG CAAGGTCAAA CACCAGCTAG TAATCCAGTC 
AAAGCCTTCT GTGAAAGCAG GGCCTACATA CCTAGTTTAT CAGCACCTGC ATTTAGTACT 
TCACTTAACT TTGTGGAAAC TGAAAAAAAT TTCAGAAAAA CTAACCACGA AATGGCAAAC 
GGTGGTAATC AGAATCCAAA GGTGGTAGAT GAAGCTCTGA AAGTAGATGA TTGTGACTGT 
CATCCTGAAT TTGTACCGCC ATCAAGTCAG CCTCCAAAAT ATAAAGGGAA ACAAAAATCT 
CGAAACAATG AATTGGAGAA GTTCAGCCCT GGTTCACAAG AAACTTTACC AGTAGATTTA 
AAAAACATCT TGGAGAAACA GTTTTCTAAA TCTTCCAGAG CCGCACACCA GGATTTTGCT 
AATATAATGA AAATGTTGAG AAGTTTAATT CAGGATGGCT ACACGGCCTT GCTGGAGCAG 
CGCTGCCGCA GCGCCGCCCA AGCCTTCACA GAGTTGCTCA ACGGTTTAGA TCCTCAAAAA 
ATAAAGCAAT TAAACCTGGC CATGATTAAC TACGTGTTAG TAGTCTACGG ACTTGCCATT 
TCACTCCTTG GAATAGGACA GCCCGAGGAG TTATCTGAAG CTGAAAACCA GTTCAAGAGG 
ATGATTGAAC ACTACCCCAA TGAGGGGCTT GATTGCTTGG CCTACTGTGG AATTGGAAAA 
GTGTATTTGA AAAAAAACAG ATTTTTAGAA GCTCTTAATC ATTTTGAGAA AGCAAGAACC 
TTGATCTGTC GTCTTCCTGG AGTGTTAACT TGGCCCACAA GTAATGTGGT TATTGAGGAG 
ACTCAGCCAG GGAAGATAAA GATGCTGTTA GAGAAATTTG TTGAAGAATG CAGGTTCCCT 
CCAGTGCCGG ATGCCACTTG TTGCTATCAG AAGTGCCATG GATATTCCAA AATCCAGATA 
TATATAACTG ATCCAGACTT TAAGGGTTTT ATACGGATCA GCTGTTGCCA GTATTGTAAA 
ATAGAATTTC ACATGAACTG CTGGAAGAAG TTAAAAACAA CAACCTTCAA TGATAAAATT 
GACAAGGATT TTCTGCAAGG AATCTGTCTT ACTCCTGATT GTGAAGGAAT CATTTCAAAG 
ATTATCATCT TCAGCAGTGG TGGTCAAGTT AAATGTGAAT TTGAACACAA GGTCATTAAA 
GAAAAGGTTC CTCCAAGACC TATTCTGAAA CAGAAATGTT CTAGCCTAGA GAAGTTGAGG 
CTGAAAGAAG ACAAAAAATT GAAGCGAAAG CTCCAAAAAA AGGAAGCAAA AAAATTAGCA 
CAAGAAAGAC TGGAAGAGGA CTTAAGGGAA AGTAACCCAC CAAAAACTGA AGAGCAGAGA 
GAAACTATCA ACCATGTCCA AAGTTGCCAG TTCCTTGATG ACAGAATTCT GCAGTGCATA 
AAGCAGTACG CTGACAAGAT TAAGTCCGGG ATACTGAATA CTTCCAAGCT TCTCAAAGAA 
TTGCTTTCTT GGAAGGTTTT AACCACAGAA GACTACACAA CGTGTTTTTC TAGCAGAAAT 
TTCCTGAATG AAGCAGTGGA CTATGTCATT CGTCATTTGA TCCAAGAAAA GAATAGAGTA 
AAGACAAGGG TATTTCTGCA TGTTTTGAGT GAGCTTAAAG AAGTGGACCC AAAACTAGCC 
ACCTGGATCC AGAAACTGAA TAGCTTTGGC TTAGATGCCA CAGGACCTTT CTTTTCTCGT 
TATGGGGCTC CTCTTAAGGA GCTTGATTTT AGCATCATGA CTTTTCTCTG GAATGAGAAA 
TACGGTCATA AGCTAGGCTC TATAGAAGGA AAGCAACTTG ATTATTTCTA TGAGCCTACA 
TCACTGAAGG AAGCGCGGTG TTTAATATGG CTGCTAGAAG AACACAGAGA CAAGTTCCCA 
GCACTGCATA ATGCCTTGGA CGAGTTCTTT GATATAATGG ACAGCCGCTG CACTGTATTA 
AGGAAACAGG ACAGTGGTGA CGTGCCATTT AATTCTACCA AGATAAAAAA CAAAGGCAAG 
AAAAAGAAGC CAAAAGATTC AAAGCCTATG TTAGTTGGAT CTGGAACACC TTCAGTCACA 
CCAAACAATG AGACTGTCAC TTCAGGTGAA GACCATATCA AACGCAGTTT AAGTTCTGCG 
GGCCCCTTTG TAGTGCCTGA TCACCTTCGG CAGGATATAG AAGAATTTGA AGCTCTGTAT 
GAACAGCATA GTAATGAGTA TGTTGTCCGT AATAAGAAGC TGTGGGACAT AAACCCAAAA 
CAGAAATGTT CAACTCTATA CGATTATTTC TCTCAGTTGT TGGAGGAACA TGGTCCCTTG 
GACATGAGTA ACAAAATGTT TTCTGAAGAA TATGAGTTCT TCCCAGAAGA AACTCAACAG 
ATACTAGAAA AAGCAGGAGG CTTGAAAGCG TTTCTCCTGG GATGTCCTCG TTTTGTTGTG 
ATTGATAACT GTATCGCATT GAAGAAAGTT GCATTACGGC TCAAGAAAAA GAGAAAGAAG 
AAAAACATTA AAACAAAAGT AGAAGAAATT TCAAAAACAG GAGAGTATTT ACGAGTTAAA 
CTACCACTGA ATCCAACTGC TAGGGAATTT AAACCAGATG TAAAGTCCAA ACCAGTGTCT 
GACTTACCTT CAGCACCAGC TTCTGAGGAC ATGAAGCCTC AGCCTGCACC TGCTAATTCT 
CCCCAATCAG CTCGTGACAC TGGGAAGCCA AAACCAGTTT TCGATAATTC TTCCAGATCA 
GTTTCTGAGG ATGAGAAGCC CAAAGGGATG TCTTCTGATT CTCCCAGGCC AGTCTTTGAG 
GATACCGGTC ACAAGCCGGT CTCCTCTAAT TCTCCCAAAC CAGTTCCTGA GGAAGTGAAA 
GCGACCTATT GGCCTCGACC CCAGTTGGTC ACAGGATACT GTACATATCT TCCCTTTCGG 
GGATTTGACA TTACCCAGAC TCCACCAGCC TTCATCAGTG TGTTACCGAC TTTGCCCCAG 
TATAGCATTT ACACCCCTCT GGCCAGTGTT CCTACTGAGT ATCACCTGCA AAGACCAATA 
CCAGTGGTGC CGTCTTTTGT AGCCAGTGAC ATAACGGAAG TGCATTTTGA GGCTCATCAT 
TTCAGTGCTG AGAATGCCCC TGGTACCCAG ATTGCCTCCG AAACACAGAT CCTTGAGGAA 
TCTTTGGAAA CACCAGTGAA GTCAGAGTGC AGCTATGATG GTGCTGATAT AGTCCAGAGT 
GAGGCTAGCA GAAACGATGA CTACTGTGGC GATTCTGCCA AATGGGAAGT AAATCCAGAA 
AGCACCAATG AAGTAACAAA TATTCCATGT ACACAAGTGG TTGCTGTACA GGTGTCTTGG 
AATATAACAC ATCAGGAAGT CAATACTGAG CCATATGGTC CTTTTGAGGC ACAACAGGGG 
GGTATTTCAC AGGTTGAAAA GGAGTACCAG GTTTTACAAG AGCAGCTTAA GGAAGCATGT 
GAAAATTATG AACACAGCAA TGTCAAGGGC TCAGAAGAAA CCAAGGACTT GGAAGAAAAG 
TTGAAAAGGA ATGTTGAAGA AAATAAGATC TCAAAGACAG AACTAGATTG GTTCCTTGAA 
GATTTGAACA AGGAAATTAA AAAATGGGAA CAGGAGAAAA AAGAAATCCA AGAAAGACTA 
AAGGCACTGA AGAAGAAAAT GAAAAAGGTG TTAAATGCCA GCAAAATGTA TACGCAGGAA 
AATGATGGAA AGGAAAAGGC ACTTGAATTA CTTCTGGATC AGTCCCTTGA AATCAGTAAT 
ACATTTACAA ATGAGAAAAT GAAAATAGAA GAGAGTATAA AGAAAGGGAA AGAGCATTAT 
GAAGAGAGTC TTCAAAGGGC TGTGGATGCA GAGGTATCTG TGCTTGAAAA CTGGAAGGAC 
ATGGAAGTGT GTAAGTTACA GAGCATGGAG TCACAAGCCG AAAGGTTTCT CAAGAATCTG 
AAGCTAATGA GCAGTGATTC GGCAGCGTAT TCTGACATGG ACTCAGACAT ACATTCATGG 
GAATCGTTTC TTTCTGATGT TAGAGAAGAA ATTGAGAAAG CAAAGTCTCA GTTTGAAGAA 
CAAATTCAGG CAATTAAATA TGGTTCTCAG CTCAGTGAAC TTCCTAAAGT GCAGATTTCT 
GAGCTTTCAT TTCCTACCTG TAACACGATT CATCCTAAAT TACTTCCTGA GTCTTCAGGC 
CAGGATGGTC AAGGTCCCTC CACTTCTACA AGTCATGGGA CCGGAGACCA GACAGCAGCT 
CCCGAGGAGT CAAGTCTGGG CTCTGCAGCT GACAGCCCAG TGGAGTTGCC CTCCTGCAGT 
CAGAAAGCCC CCCAGCTGCC AGAGCATGAG GGTGCTGGCC AGGCAACTGG GCCAGAACCA 
GGCCCAGTGG CTGATCAGAA GTTACCTGCT GCTCCAGAGC GTGCCACTCA TTCAAGCCAG 
TCTCCAAAGA AGCCGTTCAA TAGCATTATT GAGCACCTGT TGGTGGTGTT CCCGTGTTAC 
AGCAGCACGG AGCTTGCTGG TTTTATTAAA AAAGTTAGAA ACAAAAACAA GACCTCTCTG 
TCTGGATTGA GTATTGACGA AATTGTGCAA AGAGTGACAG AACACGTCCT TGATGAAGAA 
AGAAAGAAAA AGGTGTCCTG CGCAAATTCC TGTGAAATGT GCCGAGAAGT ATTCAAGTCC 
AAACACGTGC GTGAGCTGCA GTGTGGGCAC AGGCTTCACA AGGGGTGCTT CAAGCAGTGG 
CTGAAGAGCC AGCGCACCTG CCCAGCCTGC CTGGGTCACG ACCTCCTGTC AGAGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_024999804.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855572.1 Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X7, mRNA.

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CloneID	OBa218656
Clone ID Related Accession (Same CDS sequence)	XM_024999804.1
Accession Version	XM_024999804.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5787bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TTC3 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACGAA AAAAAGCATT CTTGATGAAG GTGAAAAACA TCTGTGGGTG GTGTACCATG 
GCCGATTTTC CCCAGGGAAA TTTTGCAGAT TATGCCTTGT TAGAAGATTG CCCTTATGTG 
GATGATTGTA TCTTTGCCCC TGAGTTTATG TCCAGTGATT ATGTTCGTGT GACTCAGCTT 
TACTGTGATG GAGTGGGTAG ACAGTATAAA GATTATGCCC AAACTGAGGG AAACTTAGAA 
TTTGACATCT GCACTCTGTG GTGTAGTCAA CCTATTTCTG TCCTGCAAGA TTACTGTGAT 
GCCATTAAAG TCCACATCTT CTGGCCACTT CTGTTTCAAC GTCAGCAGAG CTCTATAATA 
ACACGGATGC ACTCCTGTGT AAGGACCAGC GGTTCTCATG CTTGTGAGAT AAGTTTGAAG 
AAATTACAGC AGCTTGAACT GATGGAAGAT ATTGTGGATT TGGCAAAGAA AGTTGTGAAT 
GATACATTCT TTATCGGAGG CTTATTGAGA ATTGGCTATA AAATAGAGAA TAAAATCTTG 
GCAATGGAAG AAGCTTTGAA TTGGGTGAAA TACACAGGCG ATGTAACAAT TTTATCTAAA 
TTAGGAGCAG TTGATAATTG TTGGCGTATG TTAAGTATTT TCTTTACTGA ATACAAGTAT 
CACATAACTA AAGTTGTAAC AGAAAACTGC AGTTTGCTTG AAGAATTTAA AACTCAAAGT 
TGTGCGGAGT GTCTAGAGCA AGGAGAACTA ATGAAAATGA AAGGAAATGA AGAGTTTTCC 
AAAGAAAGAT ATGATATAGC TATTATCTAT TACACCAGAG CCATTGAATG TAGGCCTGAA 
AATCACCTGC TTTATGGTAA CCGAGCTCTT TGTTTTCTTC GTACTGGACA ATTCAGAAAT 
GCACTTGGTG ATGGAAAGAG AGCTACCATT CTGAAGAACA ATTGGACAAA GGGTCATTAT 
CGTTATTGTG CTGCTCTTTC TATGCTGGGG GAATATGACT GGGCCCTGCA AGCAAACATA 
AAAGCTCAAA AACTCTGTAA AAATGACCCT GAGGGAATCA AGGATCTAAT TCAGCAGCAT 
GTAAAGTTAC AAAAACAAAT AGAAGACCTG CAAGGTCAAA CACCAGCTAG TAATCCAGTC 
AAAGCCTTCT GTGAAAGCAG GGCCTACATA CCTAGTTTAT CAGCACCTGC ATTTAGTACT 
TCACTTAACT TTGTGGAAAC TGAAAAAAAT TTCAGAAAAA CTAACCACGA AATGGCAAAC 
GGTGGTAATC AGAATCCAAA GGTGGTAGAT GAAGCTCTGA AAGTAGATGA TTGTGACTGT 
CATCCTGAAT TTGTACCGCC ATCAAGTCAG CCTCCAAAAT ATAAAGGGAA ACAAAAATCT 
CGAAACAATG AATTGGAGAA GTTCAGCCCT GGTTCACAAG AAACTTTACC AGTAGATTTA 
AAAAACATCT TGGAGAAACA GTTTTCTAAA TCTTCCAGAG CCGCACACCA GGATTTTGCT 
AATATAATGA AAATGTTGAG AAGTTTAATT CAGGATGGCT ACACGGCCTT GCTGGAGCAG 
CGCTGCCGCA GCGCCGCCCA AGCCTTCACA GAGTTGCTCA ACGGTTTAGA TCCTCAAAAA 
ATAAAGCAAT TAAACCTGGC CATGATTAAC TACGTGTTAG TAGTCTACGG ACTTGCCATT 
TCACTCCTTG GAATAGGACA GCCCGAGGAG TTATCTGAAG CTGAAAACCA GTTCAAGAGG 
ATGATTGAAC ACTACCCCAA TGAGGGGCTT GATTGCTTGG CCTACTGTGG AATTGGAAAA 
GTGTATTTGA AAAAAAACAG ATTTTTAGAA GCTCTTAATC ATTTTGAGAA AGCAAGAACC 
TTGATCTGTC GTCTTCCTGG AGTGTTAACT TGGCCCACAA GTAATGTGGT TATTGAGGAG 
ACTCAGCCAG GGAAGATAAA GATGCTGTTA GAGAAATTTG TTGAAGAATG CAGGTTCCCT 
CCAGTGCCGG ATGCCACTTG TTGCTATCAG AAGTGCCATG GATATTCCAA AATCCAGATA 
TATATAACTG ATCCAGACTT TAAGGGTTTT ATACGGATCA GCTGTTGCCA GTATTGTAAA 
ATAGAATTTC ACATGAACTG CTGGAAGAAG TTAAAAACAA CAACCTTCAA TGATAAAATT 
GACAAGGATT TTCTGCAAGG AATCTGTCTT ACTCCTGATT GTGAAGGAAT CATTTCAAAG 
ATTATCATCT TCAGCAGTGG TGGTCAAGTT AAATGTGAAT TTGAACACAA GGTCATTAAA 
GAAAAGGTTC CTCCAAGACC TATTCTGAAA CAGAAATGTT CTAGCCTAGA GAAGTTGAGG 
CTGAAAGAAG ACAAAAAATT GAAGCGAAAG CTCCAAAAAA AGGAAGCAAA AAAATTAGCA 
CAAGAAAGAC TGGAAGAGGA CTTAAGGGAA AGTAACCCAC CAAAAACTGA AGAGCAGAGA 
GAAACTATCA ACCATGTCCA AAGTTGCCAG TTCCTTGATG ACAGAATTCT GCAGTGCATA 
AAGCAGTACG CTGACAAGAT TAAGTCCGGG ATACTGAATA CTTCCAAGCT TCTCAAAGAA 
TTGCTTTCTT GGAAGGTTTT AACCACAGAA GACTACACAA CGTGTTTTTC TAGCAGAAAT 
TTCCTGAATG AAGCAGTGGA CTATGTCATT CGTCATTTGA TCCAAGAAAA GAATAGAGTA 
AAGACAAGGG TATTTCTGCA TGTTTTGAGT GAGCTTAAAG AAGTGGACCC AAAACTAGCC 
ACCTGGATCC AGAAACTGAA TAGCTTTGGC TTAGATGCCA CAGGACCTTT CTTTTCTCGT 
TATGGGGCTC CTCTTAAGGA GCTTGATTTT AGCATCATGA CTTTTCTCTG GAATGAGAAA 
TACGGTCATA AGCTAGGCTC TATAGAAGGA AAGCAACTTG ATTATTTCTA TGAGCCTACA 
TCACTGAAGG AAGCGCGGTG TTTAATATGG CTGCTAGAAG AACACAGAGA CAAGTTCCCA 
GCACTGCATA ATGCCTTGGA CGAGTTCTTT GATATAATGG ACAGCCGCTG CACTGTATTA 
AGGAAACAGG ACAGTGGTGA CGTGCCATTT AATTCTACCA AGATAAAAAA CAAAGGCAAG 
AAAAAGAAGC CAAAAGATTC AAAGCCTATG TTAGTTGGAT CTGGAACACC TTCAGTCACA 
CCAAACAATG AGACTGTCAC TTCAGGTGAA GACCATATCA AACGCAGTTT AAGTTCTGCG 
GGCCCCTTTG TAGTGCCTGA TCACCTTCGG CAGGATATAG AAGAATTTGA AGCTCTGTAT 
GAACAGCATA GTAATGAGTA TGTTGTCCGT AATAAGAAGC TGTGGGACAT AAACCCAAAA 
CAGAAATGTT CAACTCTATA CGATTATTTC TCTCAGTTGT TGGAGGAACA TGGTCCCTTG 
GACATGAGTA ACAAAATGTT TTCTGAAGAA TATGAGTTCT TCCCAGAAGA AACTCAACAG 
ATACTAGAAA AAGCAGGAGG CTTGAAAGCG TTTCTCCTGG GATGTCCTCG TTTTGTTGTG 
ATTGATAACT GTATCGCATT GAAGAAAGTT GCATTACGGC TCAAGAAAAA GAGAAAGAAG 
AAAAACATTA AAACAAAAGT AGAAGAAATT TCAAAAACAG GAGAGTATTT ACGAGTTAAA 
CTACCACTGA ATCCAACTGC TAGGGAATTT AAACCAGATG TAAAGTCCAA ACCAGTGTCT 
GACTTACCTT CAGCACCAGC TTCTGAGGAC ATGAAGCCTC AGCCTGCACC TGCTAATTCT 
CCCCAATCAG CTCGTGACAC TGGGAAGCCA AAACCAGTTT TCGATAATTC TTCCAGATCA 
GTTTCTGAGG ATGAGAAGCC CAAAGGGATG TCTTCTGATT CTCCCAGGCC AGTCTTTGAG 
GATACCGGTC ACAAGCCGGT CTCCTCTAAT TCTCCCAAAC CAGTTCCTGA GGAAGTGAAA 
GCGACCTATT GGCCTCGACC CCAGTTGGTC ACAGGATACT GTACATATCT TCCCTTTCGG 
GGATTTGACA TTACCCAGAC TCCACCAGCC TTCATCAGTG TGTTACCGAC TTTGCCCCAG 
TATAGCATTT ACACCCCTCT GGCCAGTGTT CCTACTGAGT ATCACCTGCA AAGACCAATA 
CCAGTGGTGC CGTCTTTTGT AGCCAGTGAC ATAACGGAAG TGCATTTTGA GGCTCATCAT 
TTCAGTGCTG AGAATGCCCC TGGTACCCAG ATTGCCTCCG AAACACAGAT CCTTGAGGAA 
TCTTTGGAAA CACCAGTGAA GTCAGAGTGC AGCTATGATG GTGCTGATAT AGTCCAGAGT 
GAGGCTAGCA GAAACGATGA CTACTGTGGC GATTCTGCCA AATGGGAAGT AAATCCAGAA 
AGCACCAATG AAGTAACAAA TATTCCATGT ACACAAGTGG TTGCTGTACA GGTGTCTTGG 
AATATAACAC ATCAGGAAGT CAATACTGAG CCATATGGTC CTTTTGAGGC ACAACAGGGG 
GGTATTTCAC AGGTTGAAAA GGAGTACCAG GTTTTACAAG AGCAGCTTAA GGAAGCATGT 
GAAAATTATG AACACAGCAA TGTCAAGGGC TCAGAAGAAA CCAAGGACTT GGAAGAAAAG 
TTGAAAAGGA ATGTTGAAGA AAATAAGATC TCAAAGACAG AACTAGATTG GTTCCTTGAA 
GATTTGAACA AGGAAATTAA AAAATGGGAA CAGGAGAAAA AAGAAATCCA AGAAAGACTA 
AAGGCACTGA AGAAGAAAAT GAAAAAGGTG TTAAATGCCA GCAAAATGTA TACGCAGGAA 
AATGATGGAA AGGAAAAGGC ACTTGAATTA CTTCTGGATC AGTCCCTTGA AATCAGTAAT 
ACATTTACAA ATGAGAAAAT GAAAATAGAA GAGAGTATAA AGAAAGGGAA AGAGCATTAT 
GAAGAGAGTC TTCAAAGGGC TGTGGATGCA GAGGTATCTG TGCTTGAAAA CTGGAAGGAC 
ATGGAAGTGT GTAAGTTACA GAGCATGGAG TCACAAGCCG AAAGGTTTCT CAAGAATCTG 
AAGCTAATGA GCAGTGATTC GGCAGCGTAT TCTGACATGG ACTCAGACAT ACATTCATGG 
GAATCGTTTC TTTCTGATGT TAGAGAAGAA ATTGAGAAAG CAAAGTCTCA GTTTGAAGAA 
CAAATTCAGG CAATTAAATA TGGTTCTCAG CTCAGTGAAC TTCCTAAAGT GCAGATTTCT 
GAGCTTTCAT TTCCTACCTG TAACACGATT CATCCTAAAT TACTTCCTGA GTCTTCAGGC 
CAGGATGGTC AAGGTCCCTC CACTTCTACA AGTCATGGGA CCGGAGACCA GACAGCAGCT 
CCCGAGGAGT CAAGTCTGGG CTCTGCAGCT GACAGCCCAG TGGAGTTGCC CTCCTGCAGT 
CAGAAAGCCC CCCAGCTGCC AGAGCATGAG GGTGCTGGCC AGGCAACTGG GCCAGAACCA 
GGCCCAGTGG CTGATCAGAA GTTACCTGCT GCTCCAGAGC GTGCCACTCA TTCAAGCCAG 
TCTCCAAAGA AGCCGTTCAA TAGCATTATT GAGCACCTGT TGGTGGTGTT CCCGTGTTAC 
AGCAGCACGG AGCTTGCTGG TTTTATTAAA AAAGTTAGAA ACAAAAACAA GACCTCTCTG 
TCTGGATTGA GTATTGACGA AATTGTGCAA AGAGTGACAG AACACGTCCT TGATGAAGAA 
AGAAAGAAAA AGTGCTTCAA GCAGTGGCTG AAGAGCCAGC GCACCTGCCC AGCCTGCCTG 
GGTCACGACC TCCTGTCAGA GGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855572.1
CDS	2833..8619
Translation	MGRKKAFLMKVKNICGWCTMADFPQGNFADYALLEDCPYVDDCIFAPEFMSSDYVRVTQLYCDGVGRQYKDYAQTEGNLEFDICTLWCSQPISVLQDYCDAIKVHIFWPLLFQRQQSSIITRMHSCVRTSGSHACEISLKKLQQLELMEDIVDLAKKVVNDTFFIGGLLRIGYKIENKILAMEEALNWVKYTGDVTILSKLGAVDNCWRMLSIFFTEYKYHITKVVTENCSLLEEFKTQSCAECLEQGELMKMKGNEEFSKERYDIAIIYYTRAIECRPENHLLYGNRALCFLRTGQFRNALGDGKRATILKNNWTKGHYRYCAALSMLGEYDWALQANIKAQKLCKNDPEGIKDLIQQHVKLQKQIEDLQGQTPASNPVKAFCESRAYIPSLSAPAFSTSLNFVETEKNFRKTNHEMANGGNQNPKVVDEALKVDDCDCHPEFVPPSSQPPKYKGKQKSRNNELEKFSPGSQETLPVDLKNILEKQFSKSSRAAHQDFANIMKMLRSLIQDGYTALLEQRCRSAAQAFTELLNGLDPQKIKQLNLAMINYVLVVYGLAISLLGIGQPEELSEAENQFKRMIEHYPNEGLDCLAYCGIGKVYLKKNRFLEALNHFEKARTLICRLPGVLTWPTSNVVIEETQPGKIKMLLEKFVEECRFPPVPDATCCYQKCHGYSKIQIYITDPDFKGFIRISCCQYCKIEFHMNCWKKLKTTTFNDKIDKDFLQGICLTPDCEGIISKIIIFSSGGQVKCEFEHKVIKEKVPPRPILKQKCSSLEKLRLKEDKKLKRKLQKKEAKKLAQERLEEDLRESNPPKTEEQRETINHVQSCQFLDDRILQCIKQYADKIKSGILNTSKLLKELLSWKVLTTEDYTTCFSSRNFLNEAVDYVIRHLIQEKNRVKTRVFLHVLSELKEVDPKLATWIQKLNSFGLDATGPFFSRYGAPLKELDFSIMTFLWNEKYGHKLGSIEGKQLDYFYEPTSLKEARCLIWLLEEHRDKFPALHNALDEFFDIMDSRCTVLRKQDSGDVPFNSTKIKNKGKKKKPKDSKPMLVGSGTPSVTPNNETVTSGEDHIKRSLSSAGPFVVPDHLRQDIEEFEALYEQHSNEYVVRNKKLWDINPKQKCSTLYDYFSQLLEEHGPLDMSNKMFSEEYEFFPEETQQILEKAGGLKAFLLGCPRFVVIDNCIALKKVALRLKKKRKKKNIKTKVEEISKTGEYLRVKLPLNPTAREFKPDVKSKPVSDLPSAPASEDMKPQPAPANSPQSARDTGKPKPVFDNSSRSVSEDEKPKGMSSDSPRPVFEDTGHKPVSSNSPKPVPEEVKATYWPRPQLVTGYCTYLPFRGFDITQTPPAFISVLPTLPQYSIYTPLASVPTEYHLQRPIPVVPSFVASDITEVHFEAHHFSAENAPGTQIASETQILEESLETPVKSECSYDGADIVQSEASRNDDYCGDSAKWEVNPESTNEVTNIPCTQVVAVQVSWNITHQEVNTEPYGPFEAQQGGISQVEKEYQVLQEQLKEACENYEHSNVKGSEETKDLEEKLKRNVEENKISKTELDWFLEDLNKEIKKWEQEKKEIQERLKALKKKMKKVLNASKMYTQENDGKEKALELLLDQSLEISNTFTNEKMKIEESIKKGKEHYEESLQRAVDAEVSVLENWKDMEVCKLQSMESQAERFLKNLKLMSSDSAAYSDMDSDIHSWESFLSDVREEIEKAKSQFEEQIQAIKYGSQLSELPKVQISELSFPTCNTIHPKLLPESSGQDGQGPSTSTSHGTGDQTAAPEESSLGSAADSPVELPSCSQKAPQLPEHEGAGQATGPEPGPVADQKLPAAPERATHSSQSPKKPFNSIIEHLLVVFPCYSSTELAGFIKKVRNKNKTSLSGLSIDEIVQRVTEHVLDEERKKKCFKQWLKSQRTCPACLGHDLLSEE

Target ORF information:

RefSeq Version	XM_024999804.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999804.1

ORF Insert Sequence:

ATGGGACGAA AAAAAGCATT CTTGATGAAG GTGAAAAACA TCTGTGGGTG GTGTACCATG 
GCCGATTTTC CCCAGGGAAA TTTTGCAGAT TATGCCTTGT TAGAAGATTG CCCTTATGTG 
GATGATTGTA TCTTTGCCCC TGAGTTTATG TCCAGTGATT ATGTTCGTGT GACTCAGCTT 
TACTGTGATG GAGTGGGTAG ACAGTATAAA GATTATGCCC AAACTGAGGG AAACTTAGAA 
TTTGACATCT GCACTCTGTG GTGTAGTCAA CCTATTTCTG TCCTGCAAGA TTACTGTGAT 
GCCATTAAAG TCCACATCTT CTGGCCACTT CTGTTTCAAC GTCAGCAGAG CTCTATAATA 
ACACGGATGC ACTCCTGTGT AAGGACCAGC GGTTCTCATG CTTGTGAGAT AAGTTTGAAG 
AAATTACAGC AGCTTGAACT GATGGAAGAT ATTGTGGATT TGGCAAAGAA AGTTGTGAAT 
GATACATTCT TTATCGGAGG CTTATTGAGA ATTGGCTATA AAATAGAGAA TAAAATCTTG 
GCAATGGAAG AAGCTTTGAA TTGGGTGAAA TACACAGGCG ATGTAACAAT TTTATCTAAA 
TTAGGAGCAG TTGATAATTG TTGGCGTATG TTAAGTATTT TCTTTACTGA ATACAAGTAT 
CACATAACTA AAGTTGTAAC AGAAAACTGC AGTTTGCTTG AAGAATTTAA AACTCAAAGT 
TGTGCGGAGT GTCTAGAGCA AGGAGAACTA ATGAAAATGA AAGGAAATGA AGAGTTTTCC 
AAAGAAAGAT ATGATATAGC TATTATCTAT TACACCAGAG CCATTGAATG TAGGCCTGAA 
AATCACCTGC TTTATGGTAA CCGAGCTCTT TGTTTTCTTC GTACTGGACA ATTCAGAAAT 
GCACTTGGTG ATGGAAAGAG AGCTACCATT CTGAAGAACA ATTGGACAAA GGGTCATTAT 
CGTTATTGTG CTGCTCTTTC TATGCTGGGG GAATATGACT GGGCCCTGCA AGCAAACATA 
AAAGCTCAAA AACTCTGTAA AAATGACCCT GAGGGAATCA AGGATCTAAT TCAGCAGCAT 
GTAAAGTTAC AAAAACAAAT AGAAGACCTG CAAGGTCAAA CACCAGCTAG TAATCCAGTC 
AAAGCCTTCT GTGAAAGCAG GGCCTACATA CCTAGTTTAT CAGCACCTGC ATTTAGTACT 
TCACTTAACT TTGTGGAAAC TGAAAAAAAT TTCAGAAAAA CTAACCACGA AATGGCAAAC 
GGTGGTAATC AGAATCCAAA GGTGGTAGAT GAAGCTCTGA AAGTAGATGA TTGTGACTGT 
CATCCTGAAT TTGTACCGCC ATCAAGTCAG CCTCCAAAAT ATAAAGGGAA ACAAAAATCT 
CGAAACAATG AATTGGAGAA GTTCAGCCCT GGTTCACAAG AAACTTTACC AGTAGATTTA 
AAAAACATCT TGGAGAAACA GTTTTCTAAA TCTTCCAGAG CCGCACACCA GGATTTTGCT 
AATATAATGA AAATGTTGAG AAGTTTAATT CAGGATGGCT ACACGGCCTT GCTGGAGCAG 
CGCTGCCGCA GCGCCGCCCA AGCCTTCACA GAGTTGCTCA ACGGTTTAGA TCCTCAAAAA 
ATAAAGCAAT TAAACCTGGC CATGATTAAC TACGTGTTAG TAGTCTACGG ACTTGCCATT 
TCACTCCTTG GAATAGGACA GCCCGAGGAG TTATCTGAAG CTGAAAACCA GTTCAAGAGG 
ATGATTGAAC ACTACCCCAA TGAGGGGCTT GATTGCTTGG CCTACTGTGG AATTGGAAAA 
GTGTATTTGA AAAAAAACAG ATTTTTAGAA GCTCTTAATC ATTTTGAGAA AGCAAGAACC 
TTGATCTGTC GTCTTCCTGG AGTGTTAACT TGGCCCACAA GTAATGTGGT TATTGAGGAG 
ACTCAGCCAG GGAAGATAAA GATGCTGTTA GAGAAATTTG TTGAAGAATG CAGGTTCCCT 
CCAGTGCCGG ATGCCACTTG TTGCTATCAG AAGTGCCATG GATATTCCAA AATCCAGATA 
TATATAACTG ATCCAGACTT TAAGGGTTTT ATACGGATCA GCTGTTGCCA GTATTGTAAA 
ATAGAATTTC ACATGAACTG CTGGAAGAAG TTAAAAACAA CAACCTTCAA TGATAAAATT 
GACAAGGATT TTCTGCAAGG AATCTGTCTT ACTCCTGATT GTGAAGGAAT CATTTCAAAG 
ATTATCATCT TCAGCAGTGG TGGTCAAGTT AAATGTGAAT TTGAACACAA GGTCATTAAA 
GAAAAGGTTC CTCCAAGACC TATTCTGAAA CAGAAATGTT CTAGCCTAGA GAAGTTGAGG 
CTGAAAGAAG ACAAAAAATT GAAGCGAAAG CTCCAAAAAA AGGAAGCAAA AAAATTAGCA 
CAAGAAAGAC TGGAAGAGGA CTTAAGGGAA AGTAACCCAC CAAAAACTGA AGAGCAGAGA 
GAAACTATCA ACCATGTCCA AAGTTGCCAG TTCCTTGATG ACAGAATTCT GCAGTGCATA 
AAGCAGTACG CTGACAAGAT TAAGTCCGGG ATACTGAATA CTTCCAAGCT TCTCAAAGAA 
TTGCTTTCTT GGAAGGTTTT AACCACAGAA GACTACACAA CGTGTTTTTC TAGCAGAAAT 
TTCCTGAATG AAGCAGTGGA CTATGTCATT CGTCATTTGA TCCAAGAAAA GAATAGAGTA 
AAGACAAGGG TATTTCTGCA TGTTTTGAGT GAGCTTAAAG AAGTGGACCC AAAACTAGCC 
ACCTGGATCC AGAAACTGAA TAGCTTTGGC TTAGATGCCA CAGGACCTTT CTTTTCTCGT 
TATGGGGCTC CTCTTAAGGA GCTTGATTTT AGCATCATGA CTTTTCTCTG GAATGAGAAA 
TACGGTCATA AGCTAGGCTC TATAGAAGGA AAGCAACTTG ATTATTTCTA TGAGCCTACA 
TCACTGAAGG AAGCGCGGTG TTTAATATGG CTGCTAGAAG AACACAGAGA CAAGTTCCCA 
GCACTGCATA ATGCCTTGGA CGAGTTCTTT GATATAATGG ACAGCCGCTG CACTGTATTA 
AGGAAACAGG ACAGTGGTGA CGTGCCATTT AATTCTACCA AGATAAAAAA CAAAGGCAAG 
AAAAAGAAGC CAAAAGATTC AAAGCCTATG TTAGTTGGAT CTGGAACACC TTCAGTCACA 
CCAAACAATG AGACTGTCAC TTCAGGTGAA GACCATATCA AACGCAGTTT AAGTTCTGCG 
GGCCCCTTTG TAGTGCCTGA TCACCTTCGG CAGGATATAG AAGAATTTGA AGCTCTGTAT 
GAACAGCATA GTAATGAGTA TGTTGTCCGT AATAAGAAGC TGTGGGACAT AAACCCAAAA 
CAGAAATGTT CAACTCTATA CGATTATTTC TCTCAGTTGT TGGAGGAACA TGGTCCCTTG 
GACATGAGTA ACAAAATGTT TTCTGAAGAA TATGAGTTCT TCCCAGAAGA AACTCAACAG 
ATACTAGAAA AAGCAGGAGG CTTGAAAGCG TTTCTCCTGG GATGTCCTCG TTTTGTTGTG 
ATTGATAACT GTATCGCATT GAAGAAAGTT GCATTACGGC TCAAGAAAAA GAGAAAGAAG 
AAAAACATTA AAACAAAAGT AGAAGAAATT TCAAAAACAG GAGAGTATTT ACGAGTTAAA 
CTACCACTGA ATCCAACTGC TAGGGAATTT AAACCAGATG TAAAGTCCAA ACCAGTGTCT 
GACTTACCTT CAGCACCAGC TTCTGAGGAC ATGAAGCCTC AGCCTGCACC TGCTAATTCT 
CCCCAATCAG CTCGTGACAC TGGGAAGCCA AAACCAGTTT TCGATAATTC TTCCAGATCA 
GTTTCTGAGG ATGAGAAGCC CAAAGGGATG TCTTCTGATT CTCCCAGGCC AGTCTTTGAG 
GATACCGGTC ACAAGCCGGT CTCCTCTAAT TCTCCCAAAC CAGTTCCTGA GGAAGTGAAA 
GCGACCTATT GGCCTCGACC CCAGTTGGTC ACAGGATACT GTACATATCT TCCCTTTCGG 
GGATTTGACA TTACCCAGAC TCCACCAGCC TTCATCAGTG TGTTACCGAC TTTGCCCCAG 
TATAGCATTT ACACCCCTCT GGCCAGTGTT CCTACTGAGT ATCACCTGCA AAGACCAATA 
CCAGTGGTGC CGTCTTTTGT AGCCAGTGAC ATAACGGAAG TGCATTTTGA GGCTCATCAT 
TTCAGTGCTG AGAATGCCCC TGGTACCCAG ATTGCCTCCG AAACACAGAT CCTTGAGGAA 
TCTTTGGAAA CACCAGTGAA GTCAGAGTGC AGCTATGATG GTGCTGATAT AGTCCAGAGT 
GAGGCTAGCA GAAACGATGA CTACTGTGGC GATTCTGCCA AATGGGAAGT AAATCCAGAA 
AGCACCAATG AAGTAACAAA TATTCCATGT ACACAAGTGG TTGCTGTACA GGTGTCTTGG 
AATATAACAC ATCAGGAAGT CAATACTGAG CCATATGGTC CTTTTGAGGC ACAACAGGGG 
GGTATTTCAC AGGTTGAAAA GGAGTACCAG GTTTTACAAG AGCAGCTTAA GGAAGCATGT 
GAAAATTATG AACACAGCAA TGTCAAGGGC TCAGAAGAAA CCAAGGACTT GGAAGAAAAG 
TTGAAAAGGA ATGTTGAAGA AAATAAGATC TCAAAGACAG AACTAGATTG GTTCCTTGAA 
GATTTGAACA AGGAAATTAA AAAATGGGAA CAGGAGAAAA AAGAAATCCA AGAAAGACTA 
AAGGCACTGA AGAAGAAAAT GAAAAAGGTG TTAAATGCCA GCAAAATGTA TACGCAGGAA 
AATGATGGAA AGGAAAAGGC ACTTGAATTA CTTCTGGATC AGTCCCTTGA AATCAGTAAT 
ACATTTACAA ATGAGAAAAT GAAAATAGAA GAGAGTATAA AGAAAGGGAA AGAGCATTAT 
GAAGAGAGTC TTCAAAGGGC TGTGGATGCA GAGGTATCTG TGCTTGAAAA CTGGAAGGAC 
ATGGAAGTGT GTAAGTTACA GAGCATGGAG TCACAAGCCG AAAGGTTTCT CAAGAATCTG 
AAGCTAATGA GCAGTGATTC GGCAGCGTAT TCTGACATGG ACTCAGACAT ACATTCATGG 
GAATCGTTTC TTTCTGATGT TAGAGAAGAA ATTGAGAAAG CAAAGTCTCA GTTTGAAGAA 
CAAATTCAGG CAATTAAATA TGGTTCTCAG CTCAGTGAAC TTCCTAAAGT GCAGATTTCT 
GAGCTTTCAT TTCCTACCTG TAACACGATT CATCCTAAAT TACTTCCTGA GTCTTCAGGC 
CAGGATGGTC AAGGTCCCTC CACTTCTACA AGTCATGGGA CCGGAGACCA GACAGCAGCT 
CCCGAGGAGT CAAGTCTGGG CTCTGCAGCT GACAGCCCAG TGGAGTTGCC CTCCTGCAGT 
CAGAAAGCCC CCCAGCTGCC AGAGCATGAG GGTGCTGGCC AGGCAACTGG GCCAGAACCA 
GGCCCAGTGG CTGATCAGAA GTTACCTGCT GCTCCAGAGC GTGCCACTCA TTCAAGCCAG 
TCTCCAAAGA AGCCGTTCAA TAGCATTATT GAGCACCTGT TGGTGGTGTT CCCGTGTTAC 
AGCAGCACGG AGCTTGCTGG TTTTATTAAA AAAGTTAGAA ACAAAAACAA GACCTCTCTG 
TCTGGATTGA GTATTGACGA AATTGTGCAA AGAGTGACAG AACACGTCCT TGATGAAGAA 
AGAAAGAAAA AGTGCTTCAA GCAGTGGCTG AAGAGCCAGC GCACCTGCCC AGCCTGCCTG 
GGTCACGACC TCCTGTCAGA GGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_024999841.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855609.1 Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X8, mRNA.

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CloneID	OBa218657
Clone ID Related Accession (Same CDS sequence)	XM_024999841.1
Accession Version	XM_024999841.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5649bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TTC3 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTCCT GTGTAAGGAC CAGCGGTTCT CATGCTTGTG AGATAAGTTT GAAGAAATTA 
CAGCAGCTTG AACTGATGGA AGATATTGTG GATTTGGCAA AGAAAGTTGT GAATGATACA 
TTCTTTATCG GAGGCTTATT GAGAATTGGC TATAAAATAG AGAATAAAAT CTTGGCAATG 
GAAGAAGCTT TGAATTGGGT GAAATACACA GGCGATGTAA CAATTTTATC TAAATTAGGA 
GCAGTTGATA ATTGTTGGCG TATGTTAAGT ATTTTCTTTA CTGAATACAA GTATCACATA 
ACTAAAGTTG TAACAGAAAA CTGCAGTTTG CTTGAAGAAT TTAAAACTCA AAGTTGTGCG 
GAGTGTCTAG AGCAAGGAGA ACTAATGAAA ATGAAAGGAA ATGAAGAGTT TTCCAAAGAA 
AGATATGATA TAGCTATTAT CTATTACACC AGAGCCATTG AATGTAGGCC TGAAAATCAC 
CTGCTTTATG GTAACCGAGC TCTTTGTTTT CTTCGTACTG GACAATTCAG AAATGCACTT 
GGTGATGGAA AGAGAGCTAC CATTCTGAAG AACAATTGGA CAAAGGGTCA TTATCGTTAT 
TGTGCTGCTC TTTCTATGCT GGGGGAATAT GACTGGGCCC TGCAAGCAAA CATAAAAGCT 
CAAAAACTCT GTAAAAATGA CCCTGAGGGA ATCAAGGATC TAATTCAGCA GCATGTAAAG 
TTACAAAAAC AAATAGAAGA CCTGCAAGGT CAAACACCAG CTAGTAATCC AGTCAAAGCC 
TTCTGTGAAA GCAGGGCCTA CATACCTAGT TTATCAGCAC CTGCATTTAG TACTTCACTT 
AACTTTGTGG AAACTGAAAA AAATTTCAGA AAAACTAACC ACGAAATGGC AAACGGTGGT 
AATCAGAATC CAAAGGTGGT AGATGAAGCT CTGAAAGTAG ATGATTGTGA CTGTCATCCT 
GAATTTGTAC CGCCATCAAG TCAGCCTCCA AAATATAAAG GGAAACAAAA ATCTCGAAAC 
AATGAATTGG AGAAGTTCAG CCCTGGTTCA CAAGAAACTT TACCAGTAGA TTTAAAAAAC 
ATCTTGGAGA AACAGTTTTC TAAATCTTCC AGAGCCGCAC ACCAGGATTT TGCTAATATA 
ATGAAAATGT TGAGAAGTTT AATTCAGGAT GGCTACACGG CCTTGCTGGA GCAGCGCTGC 
CGCAGCGCCG CCCAAGCCTT CACAGAGTTG CTCAACGGTT TAGATCCTCA AAAAATAAAG 
CAATTAAACC TGGCCATGAT TAACTACGTG TTAGTAGTCT ACGGACTTGC CATTTCACTC 
CTTGGAATAG GACAGCCCGA GGAGTTATCT GAAGCTGAAA ACCAGTTCAA GAGGATGATT 
GAACACTACC CCAATGAGGG GCTTGATTGC TTGGCCTACT GTGGAATTGG AAAAGTGTAT 
TTGAAAAAAA ACAGATTTTT AGAAGCTCTT AATCATTTTG AGAAAGCAAG AACCTTGATC 
TGTCGTCTTC CTGGAGTGTT AACTTGGCCC ACAAGTAATG TGGTTATTGA GGAGACTCAG 
CCAGGGAAGA TAAAGATGCT GTTAGAGAAA TTTGTTGAAG AATGCAGGTT CCCTCCAGTG 
CCGGATGCCA CTTGTTGCTA TCAGAAGTGC CATGGATATT CCAAAATCCA GATATATATA 
ACTGATCCAG ACTTTAAGGG TTTTATACGG ATCAGCTGTT GCCAGTATTG TAAAATAGAA 
TTTCACATGA ACTGCTGGAA GAAGTTAAAA ACAACAACCT TCAATGATAA AATTGACAAG 
GATTTTCTGC AAGGAATCTG TCTTACTCCT GATTGTGAAG GAATCATTTC AAAGATTATC 
ATCTTCAGCA GTGGTGGTCA AGTTAAATGT GAATTTGAAC ACAAGGTCAT TAAAGAAAAG 
GTTCCTCCAA GACCTATTCT GAAACAGAAA TGTTCTAGCC TAGAGAAGTT GAGGCTGAAA 
GAAGACAAAA AATTGAAGCG AAAGCTCCAA AAAAAGGAAG CAAAAAAATT AGCACAAGAA 
AGACTGGAAG AGGACTTAAG GGAAAGTAAC CCACCAAAAA CTGAAGAGCA GAGAGAAACT 
ATCAACCATG TCCAAAGTTG CCAGTTCCTT GATGACAGAA TTCTGCAGTG CATAAAGCAG 
TACGCTGACA AGATTAAGTC CGGGATACTG AATACTTCCA AGCTTCTCAA AGAATTGCTT 
TCTTGGAAGG TTTTAACCAC AGAAGACTAC ACAACGTGTT TTTCTAGCAG AAATTTCCTG 
AATGAAGCAG TGGACTATGT CATTCGTCAT TTGATCCAAG AAAAGAATAG AGTAAAGACA 
AGGGTATTTC TGCATGTTTT GAGTGAGCTT AAAGAAGTGG ACCCAAAACT AGCCACCTGG 
ATCCAGAAAC TGAATAGCTT TGGCTTAGAT GCCACAGGAC CTTTCTTTTC TCGTTATGGG 
GCTCCTCTTA AGGAGCTTGA TTTTAGCATC ATGACTTTTC TCTGGAATGA GAAATACGGT 
CATAAGCTAG GCTCTATAGA AGGAAAGCAA CTTGATTATT TCTATGAGCC TACATCACTG 
AAGGAAGCGC GGTGTTTAAT ATGGCTGCTA GAAGAACACA GAGACAAGTT CCCAGCACTG 
CATAATGCCT TGGACGAGTT CTTTGATATA ATGGACAGCC GCTGCACTGT ATTAAGGAAA 
CAGGACAGTG GTGACGTGCC ATTTAATTCT ACCAAGATAA AAAACAAAGG CAAGAAAAAG 
AAGCCAAAAG ATTCAAAGCC TATGTTAGTT GGATCTGGAA CACCTTCAGT CACACCAAAC 
AATGAGACTG TCACTTCAGG TGAAGACCAT ATCAAACGCA GTTTAAGTTC TGCGGGCCCC 
TTTGTAGTGC CTGATCACCT TCGGCAGGAT ATAGAAGAAT TTGAAGCTCT GTATGAACAG 
CATAGTAATG AGTATGTTGT CCGTAATAAG AAGCTGTGGG ACATAAACCC AAAACAGAAA 
TGTTCAACTC TATACGATTA TTTCTCTCAG TTGTTGGAGG AACATGGTCC CTTGGACATG 
AGTAACAAAA TGTTTTCTGA AGAATATGAG TTCTTCCCAG AAGAAACTCA ACAGATACTA 
GAAAAAGCAG GAGGCTTGAA AGCGTTTCTC CTGGGATGTC CTCGTTTTGT TGTGATTGAT 
AACTGTATCG CATTGAAGAA AGTTGCATTA CGGCTCAAGA AAAAGAGAAA GAAGAAAAAC 
ATTAAAACAA AAGTAGAAGA AATTTCAAAA ACAGGAGAGT ATTTACGAGT TAAACTACCA 
CTGAATCCAA CTGCTAGGGA ATTTAAACCA GATGTAAAGT CCAAACCAGT GTCTGACTTA 
CCTTCAGCAC CAGCTTCTGA GGACATGAAG CCTCAGCCTG CACCTGCTAA TTCTCCCCAA 
TCAGCTCGTG ACACTGGGAA GCCAAAACCA GTTTTCGATA ATTCTTCCAG ATCAGTTTCT 
GAGGATGAGA AGCCCAAAGG GATGTCTTCT GATTCTCCCA GGCCAGTCTT TGAGGATACC 
GGTCACAAGC CGGTCTCCTC TAATTCTCCC AAACCAGTTC CTGAGGAAGT GAAAGCGACC 
TATTGGCCTC GACCCCAGTT GGTCACAGGA TACTGTACAT ATCTTCCCTT TCGGGGATTT 
GACATTACCC AGACTCCACC AGCCTTCATC AGTGTGTTAC CGACTTTGCC CCAGTATAGC 
ATTTACACCC CTCTGGCCAG TGTTCCTACT GAGTATCACC TGCAAAGACC AATACCAGTG 
GTGCCGTCTT TTGTAGCCAG TGACATAACG GAAGTGCATT TTGAGGCTCA TCATTTCAGT 
GCTGAGAATG CCCCTGGTAC CCAGATTGCC TCCGAAACAC AGATCCTTGA GGAATCTTTG 
GAAACACCAG TGAAGTCAGA GTGCAGCTAT GATGGTGCTG ATATAGTCCA GAGTGAGGCT 
AGCAGAAACG ATGACTACTG TGGCGATTCT GCCAAATGGG AAGTAAATCC AGAAAGCACC 
AATGAAGTAA CAAATATTCC ATGTACACAA GTGGTTGCTG TACAGGTGTC TTGGAATATA 
ACACATCAGG AAGTCAATAC TGAGCCATAT GGTCCTTTTG AGGCACAACA GGGGGGTATT 
TCACAGGTTG AAAAGGAGTA CCAGGTTTTA CAAGAGCAGC TTAAGGAAGC ATGTGAAAAT 
TATGAACACA GCAATGTCAA GGGCTCAGAA GAAACCAAGG ACTTGGAAGA AAAGTTGAAA 
AGGAATGTTG AAGAAAATAA GATCTCAAAG ACAGAACTAG ATTGGTTCCT TGAAGATTTG 
AACAAGGAAA TTAAAAAATG GGAACAGGAG AAAAAAGAAA TCCAAGAAAG ACTAAAGGCA 
CTGAAGAAGA AAATGAAAAA GGTGTTAAAT GCCAGCAAAA TGTATACGCA GGAAAATGAT 
GGAAAGGAAA AGGCACTTGA ATTACTTCTG GATCAGTCCC TTGAAATCAG TAATACATTT 
ACAAATGAGA AAATGAAAAT AGAAGAGAGT ATAAAGAAAG GGAAAGAGCA TTATGAAGAG 
AGTCTTCAAA GGGCTGTGGA TGCAGAGGTA TCTGTGCTTG AAAACTGGAA GGACATGGAA 
GTGTGTAAGT TACAGAGCAT GGAGTCACAA GCCGAAAGGT TTCTCAAGAA TCTGAAGCTA 
ATGAGCAGTG ATTCGGCAGC GTATTCTGAC ATGGACTCAG ACATACATTC ATGGGAATCG 
TTTCTTTCTG ATGTTAGAGA AGAAATTGAG AAAGCAAAGT CTCAGTTTGA AGAACAAATT 
CAGGCAATTA AATATGGTTC TCAGCTCAGT GAACTTCCTA AAGTGCAGAT TTCTGAGCTT 
TCATTTCCTA CCTGTAACAC GATTCATCCT AAATTACTTC CTGAGTCTTC AGGCCAGGAT 
GGTCAAGGTC CCTCCACTTC TACAAGTCAT GGGACCGGAG ACCAGACAGC AGCTCCCGAG 
GAGTCAAGTC TGGGCTCTGC AGCTGACAGC CCAGTGGAGT TGCCCTCCTG CAGTCAGAAA 
GCCCCCCAGC TGCCAGAGCA TGAGGGTGCT GGCCAGGCAA CTGGGCCAGA ACCAGGCCCA 
GTGGCTGATC AGAAGTTACC TGCTGCTCCA GAGCGTGCCA CTCATTCAAG CCAGTCTCCA 
AAGAAGCCGT TCAATAGCAT TATTGAGCAC CTGTTGGTGG TGTTCCCGTG TTACAGCAGC 
ACGGAGCTTG CTGGTTTTAT TAAAAAAGTT AGAAACAAAA ACAAGACCTC TCTGTCTGGA 
TTGAGTATTG ACGAAATTGT GCAAAGAGTG ACAGAACACG TCCTTGATGA AGAAAGAAAG 
AAAAAGCCAA GTCCAGGAAA GGGCTGGAGG CTGTCTGCAC CCAGCTCTGC TGTTGCTGTG 
CCCAGGGCTG CCCAGGACCC GCCCTCCATG CCTGTCGGAG CATCGCCCGA AACCAGGGGG 
CCGAGGACCG AGGACGTCCC TGTGTCCTGC GCAAATTCCT GTGAAATGTG CCGAGAAGTA 
TTCAAGTCCA AACACGTGCG TGAGCTGCAG TGTGGGCACA GGCTTCACAA GGGGTGCTTC 
AAGCAGTGGC TGAAGAGCCA GCGCACCTGC CCAGCCTGCC TGGGTCACGA CCTCCTGTCA 
GAGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855609.1
CDS	455..6103
Translation	MHSCVRTSGSHACEISLKKLQQLELMEDIVDLAKKVVNDTFFIGGLLRIGYKIENKILAMEEALNWVKYTGDVTILSKLGAVDNCWRMLSIFFTEYKYHITKVVTENCSLLEEFKTQSCAECLEQGELMKMKGNEEFSKERYDIAIIYYTRAIECRPENHLLYGNRALCFLRTGQFRNALGDGKRATILKNNWTKGHYRYCAALSMLGEYDWALQANIKAQKLCKNDPEGIKDLIQQHVKLQKQIEDLQGQTPASNPVKAFCESRAYIPSLSAPAFSTSLNFVETEKNFRKTNHEMANGGNQNPKVVDEALKVDDCDCHPEFVPPSSQPPKYKGKQKSRNNELEKFSPGSQETLPVDLKNILEKQFSKSSRAAHQDFANIMKMLRSLIQDGYTALLEQRCRSAAQAFTELLNGLDPQKIKQLNLAMINYVLVVYGLAISLLGIGQPEELSEAENQFKRMIEHYPNEGLDCLAYCGIGKVYLKKNRFLEALNHFEKARTLICRLPGVLTWPTSNVVIEETQPGKIKMLLEKFVEECRFPPVPDATCCYQKCHGYSKIQIYITDPDFKGFIRISCCQYCKIEFHMNCWKKLKTTTFNDKIDKDFLQGICLTPDCEGIISKIIIFSSGGQVKCEFEHKVIKEKVPPRPILKQKCSSLEKLRLKEDKKLKRKLQKKEAKKLAQERLEEDLRESNPPKTEEQRETINHVQSCQFLDDRILQCIKQYADKIKSGILNTSKLLKELLSWKVLTTEDYTTCFSSRNFLNEAVDYVIRHLIQEKNRVKTRVFLHVLSELKEVDPKLATWIQKLNSFGLDATGPFFSRYGAPLKELDFSIMTFLWNEKYGHKLGSIEGKQLDYFYEPTSLKEARCLIWLLEEHRDKFPALHNALDEFFDIMDSRCTVLRKQDSGDVPFNSTKIKNKGKKKKPKDSKPMLVGSGTPSVTPNNETVTSGEDHIKRSLSSAGPFVVPDHLRQDIEEFEALYEQHSNEYVVRNKKLWDINPKQKCSTLYDYFSQLLEEHGPLDMSNKMFSEEYEFFPEETQQILEKAGGLKAFLLGCPRFVVIDNCIALKKVALRLKKKRKKKNIKTKVEEISKTGEYLRVKLPLNPTAREFKPDVKSKPVSDLPSAPASEDMKPQPAPANSPQSARDTGKPKPVFDNSSRSVSEDEKPKGMSSDSPRPVFEDTGHKPVSSNSPKPVPEEVKATYWPRPQLVTGYCTYLPFRGFDITQTPPAFISVLPTLPQYSIYTPLASVPTEYHLQRPIPVVPSFVASDITEVHFEAHHFSAENAPGTQIASETQILEESLETPVKSECSYDGADIVQSEASRNDDYCGDSAKWEVNPESTNEVTNIPCTQVVAVQVSWNITHQEVNTEPYGPFEAQQGGISQVEKEYQVLQEQLKEACENYEHSNVKGSEETKDLEEKLKRNVEENKISKTELDWFLEDLNKEIKKWEQEKKEIQERLKALKKKMKKVLNASKMYTQENDGKEKALELLLDQSLEISNTFTNEKMKIEESIKKGKEHYEESLQRAVDAEVSVLENWKDMEVCKLQSMESQAERFLKNLKLMSSDSAAYSDMDSDIHSWESFLSDVREEIEKAKSQFEEQIQAIKYGSQLSELPKVQISELSFPTCNTIHPKLLPESSGQDGQGPSTSTSHGTGDQTAAPEESSLGSAADSPVELPSCSQKAPQLPEHEGAGQATGPEPGPVADQKLPAAPERATHSSQSPKKPFNSIIEHLLVVFPCYSSTELAGFIKKVRNKNKTSLSGLSIDEIVQRVTEHVLDEERKKKPSPGKGWRLSAPSSAVAVPRAAQDPPSMPVGASPETRGPRTEDVPVSCANSCEMCREVFKSKHVRELQCGHRLHKGCFKQWLKSQRTCPACLGHDLLSEE

Target ORF information:

RefSeq Version	XM_024999841.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999841.1

ORF Insert Sequence:

ATGCACTCCT GTGTAAGGAC CAGCGGTTCT CATGCTTGTG AGATAAGTTT GAAGAAATTA 
CAGCAGCTTG AACTGATGGA AGATATTGTG GATTTGGCAA AGAAAGTTGT GAATGATACA 
TTCTTTATCG GAGGCTTATT GAGAATTGGC TATAAAATAG AGAATAAAAT CTTGGCAATG 
GAAGAAGCTT TGAATTGGGT GAAATACACA GGCGATGTAA CAATTTTATC TAAATTAGGA 
GCAGTTGATA ATTGTTGGCG TATGTTAAGT ATTTTCTTTA CTGAATACAA GTATCACATA 
ACTAAAGTTG TAACAGAAAA CTGCAGTTTG CTTGAAGAAT TTAAAACTCA AAGTTGTGCG 
GAGTGTCTAG AGCAAGGAGA ACTAATGAAA ATGAAAGGAA ATGAAGAGTT TTCCAAAGAA 
AGATATGATA TAGCTATTAT CTATTACACC AGAGCCATTG AATGTAGGCC TGAAAATCAC 
CTGCTTTATG GTAACCGAGC TCTTTGTTTT CTTCGTACTG GACAATTCAG AAATGCACTT 
GGTGATGGAA AGAGAGCTAC CATTCTGAAG AACAATTGGA CAAAGGGTCA TTATCGTTAT 
TGTGCTGCTC TTTCTATGCT GGGGGAATAT GACTGGGCCC TGCAAGCAAA CATAAAAGCT 
CAAAAACTCT GTAAAAATGA CCCTGAGGGA ATCAAGGATC TAATTCAGCA GCATGTAAAG 
TTACAAAAAC AAATAGAAGA CCTGCAAGGT CAAACACCAG CTAGTAATCC AGTCAAAGCC 
TTCTGTGAAA GCAGGGCCTA CATACCTAGT TTATCAGCAC CTGCATTTAG TACTTCACTT 
AACTTTGTGG AAACTGAAAA AAATTTCAGA AAAACTAACC ACGAAATGGC AAACGGTGGT 
AATCAGAATC CAAAGGTGGT AGATGAAGCT CTGAAAGTAG ATGATTGTGA CTGTCATCCT 
GAATTTGTAC CGCCATCAAG TCAGCCTCCA AAATATAAAG GGAAACAAAA ATCTCGAAAC 
AATGAATTGG AGAAGTTCAG CCCTGGTTCA CAAGAAACTT TACCAGTAGA TTTAAAAAAC 
ATCTTGGAGA AACAGTTTTC TAAATCTTCC AGAGCCGCAC ACCAGGATTT TGCTAATATA 
ATGAAAATGT TGAGAAGTTT AATTCAGGAT GGCTACACGG CCTTGCTGGA GCAGCGCTGC 
CGCAGCGCCG CCCAAGCCTT CACAGAGTTG CTCAACGGTT TAGATCCTCA AAAAATAAAG 
CAATTAAACC TGGCCATGAT TAACTACGTG TTAGTAGTCT ACGGACTTGC CATTTCACTC 
CTTGGAATAG GACAGCCCGA GGAGTTATCT GAAGCTGAAA ACCAGTTCAA GAGGATGATT 
GAACACTACC CCAATGAGGG GCTTGATTGC TTGGCCTACT GTGGAATTGG AAAAGTGTAT 
TTGAAAAAAA ACAGATTTTT AGAAGCTCTT AATCATTTTG AGAAAGCAAG AACCTTGATC 
TGTCGTCTTC CTGGAGTGTT AACTTGGCCC ACAAGTAATG TGGTTATTGA GGAGACTCAG 
CCAGGGAAGA TAAAGATGCT GTTAGAGAAA TTTGTTGAAG AATGCAGGTT CCCTCCAGTG 
CCGGATGCCA CTTGTTGCTA TCAGAAGTGC CATGGATATT CCAAAATCCA GATATATATA 
ACTGATCCAG ACTTTAAGGG TTTTATACGG ATCAGCTGTT GCCAGTATTG TAAAATAGAA 
TTTCACATGA ACTGCTGGAA GAAGTTAAAA ACAACAACCT TCAATGATAA AATTGACAAG 
GATTTTCTGC AAGGAATCTG TCTTACTCCT GATTGTGAAG GAATCATTTC AAAGATTATC 
ATCTTCAGCA GTGGTGGTCA AGTTAAATGT GAATTTGAAC ACAAGGTCAT TAAAGAAAAG 
GTTCCTCCAA GACCTATTCT GAAACAGAAA TGTTCTAGCC TAGAGAAGTT GAGGCTGAAA 
GAAGACAAAA AATTGAAGCG AAAGCTCCAA AAAAAGGAAG CAAAAAAATT AGCACAAGAA 
AGACTGGAAG AGGACTTAAG GGAAAGTAAC CCACCAAAAA CTGAAGAGCA GAGAGAAACT 
ATCAACCATG TCCAAAGTTG CCAGTTCCTT GATGACAGAA TTCTGCAGTG CATAAAGCAG 
TACGCTGACA AGATTAAGTC CGGGATACTG AATACTTCCA AGCTTCTCAA AGAATTGCTT 
TCTTGGAAGG TTTTAACCAC AGAAGACTAC ACAACGTGTT TTTCTAGCAG AAATTTCCTG 
AATGAAGCAG TGGACTATGT CATTCGTCAT TTGATCCAAG AAAAGAATAG AGTAAAGACA 
AGGGTATTTC TGCATGTTTT GAGTGAGCTT AAAGAAGTGG ACCCAAAACT AGCCACCTGG 
ATCCAGAAAC TGAATAGCTT TGGCTTAGAT GCCACAGGAC CTTTCTTTTC TCGTTATGGG 
GCTCCTCTTA AGGAGCTTGA TTTTAGCATC ATGACTTTTC TCTGGAATGA GAAATACGGT 
CATAAGCTAG GCTCTATAGA AGGAAAGCAA CTTGATTATT TCTATGAGCC TACATCACTG 
AAGGAAGCGC GGTGTTTAAT ATGGCTGCTA GAAGAACACA GAGACAAGTT CCCAGCACTG 
CATAATGCCT TGGACGAGTT CTTTGATATA ATGGACAGCC GCTGCACTGT ATTAAGGAAA 
CAGGACAGTG GTGACGTGCC ATTTAATTCT ACCAAGATAA AAAACAAAGG CAAGAAAAAG 
AAGCCAAAAG ATTCAAAGCC TATGTTAGTT GGATCTGGAA CACCTTCAGT CACACCAAAC 
AATGAGACTG TCACTTCAGG TGAAGACCAT ATCAAACGCA GTTTAAGTTC TGCGGGCCCC 
TTTGTAGTGC CTGATCACCT TCGGCAGGAT ATAGAAGAAT TTGAAGCTCT GTATGAACAG 
CATAGTAATG AGTATGTTGT CCGTAATAAG AAGCTGTGGG ACATAAACCC AAAACAGAAA 
TGTTCAACTC TATACGATTA TTTCTCTCAG TTGTTGGAGG AACATGGTCC CTTGGACATG 
AGTAACAAAA TGTTTTCTGA AGAATATGAG TTCTTCCCAG AAGAAACTCA ACAGATACTA 
GAAAAAGCAG GAGGCTTGAA AGCGTTTCTC CTGGGATGTC CTCGTTTTGT TGTGATTGAT 
AACTGTATCG CATTGAAGAA AGTTGCATTA CGGCTCAAGA AAAAGAGAAA GAAGAAAAAC 
ATTAAAACAA AAGTAGAAGA AATTTCAAAA ACAGGAGAGT ATTTACGAGT TAAACTACCA 
CTGAATCCAA CTGCTAGGGA ATTTAAACCA GATGTAAAGT CCAAACCAGT GTCTGACTTA 
CCTTCAGCAC CAGCTTCTGA GGACATGAAG CCTCAGCCTG CACCTGCTAA TTCTCCCCAA 
TCAGCTCGTG ACACTGGGAA GCCAAAACCA GTTTTCGATA ATTCTTCCAG ATCAGTTTCT 
GAGGATGAGA AGCCCAAAGG GATGTCTTCT GATTCTCCCA GGCCAGTCTT TGAGGATACC 
GGTCACAAGC CGGTCTCCTC TAATTCTCCC AAACCAGTTC CTGAGGAAGT GAAAGCGACC 
TATTGGCCTC GACCCCAGTT GGTCACAGGA TACTGTACAT ATCTTCCCTT TCGGGGATTT 
GACATTACCC AGACTCCACC AGCCTTCATC AGTGTGTTAC CGACTTTGCC CCAGTATAGC 
ATTTACACCC CTCTGGCCAG TGTTCCTACT GAGTATCACC TGCAAAGACC AATACCAGTG 
GTGCCGTCTT TTGTAGCCAG TGACATAACG GAAGTGCATT TTGAGGCTCA TCATTTCAGT 
GCTGAGAATG CCCCTGGTAC CCAGATTGCC TCCGAAACAC AGATCCTTGA GGAATCTTTG 
GAAACACCAG TGAAGTCAGA GTGCAGCTAT GATGGTGCTG ATATAGTCCA GAGTGAGGCT 
AGCAGAAACG ATGACTACTG TGGCGATTCT GCCAAATGGG AAGTAAATCC AGAAAGCACC 
AATGAAGTAA CAAATATTCC ATGTACACAA GTGGTTGCTG TACAGGTGTC TTGGAATATA 
ACACATCAGG AAGTCAATAC TGAGCCATAT GGTCCTTTTG AGGCACAACA GGGGGGTATT 
TCACAGGTTG AAAAGGAGTA CCAGGTTTTA CAAGAGCAGC TTAAGGAAGC ATGTGAAAAT 
TATGAACACA GCAATGTCAA GGGCTCAGAA GAAACCAAGG ACTTGGAAGA AAAGTTGAAA 
AGGAATGTTG AAGAAAATAA GATCTCAAAG ACAGAACTAG ATTGGTTCCT TGAAGATTTG 
AACAAGGAAA TTAAAAAATG GGAACAGGAG AAAAAAGAAA TCCAAGAAAG ACTAAAGGCA 
CTGAAGAAGA AAATGAAAAA GGTGTTAAAT GCCAGCAAAA TGTATACGCA GGAAAATGAT 
GGAAAGGAAA AGGCACTTGA ATTACTTCTG GATCAGTCCC TTGAAATCAG TAATACATTT 
ACAAATGAGA AAATGAAAAT AGAAGAGAGT ATAAAGAAAG GGAAAGAGCA TTATGAAGAG 
AGTCTTCAAA GGGCTGTGGA TGCAGAGGTA TCTGTGCTTG AAAACTGGAA GGACATGGAA 
GTGTGTAAGT TACAGAGCAT GGAGTCACAA GCCGAAAGGT TTCTCAAGAA TCTGAAGCTA 
ATGAGCAGTG ATTCGGCAGC GTATTCTGAC ATGGACTCAG ACATACATTC ATGGGAATCG 
TTTCTTTCTG ATGTTAGAGA AGAAATTGAG AAAGCAAAGT CTCAGTTTGA AGAACAAATT 
CAGGCAATTA AATATGGTTC TCAGCTCAGT GAACTTCCTA AAGTGCAGAT TTCTGAGCTT 
TCATTTCCTA CCTGTAACAC GATTCATCCT AAATTACTTC CTGAGTCTTC AGGCCAGGAT 
GGTCAAGGTC CCTCCACTTC TACAAGTCAT GGGACCGGAG ACCAGACAGC AGCTCCCGAG 
GAGTCAAGTC TGGGCTCTGC AGCTGACAGC CCAGTGGAGT TGCCCTCCTG CAGTCAGAAA 
GCCCCCCAGC TGCCAGAGCA TGAGGGTGCT GGCCAGGCAA CTGGGCCAGA ACCAGGCCCA 
GTGGCTGATC AGAAGTTACC TGCTGCTCCA GAGCGTGCCA CTCATTCAAG CCAGTCTCCA 
AAGAAGCCGT TCAATAGCAT TATTGAGCAC CTGTTGGTGG TGTTCCCGTG TTACAGCAGC 
ACGGAGCTTG CTGGTTTTAT TAAAAAAGTT AGAAACAAAA ACAAGACCTC TCTGTCTGGA 
TTGAGTATTG ACGAAATTGT GCAAAGAGTG ACAGAACACG TCCTTGATGA AGAAAGAAAG 
AAAAAGCCAA GTCCAGGAAA GGGCTGGAGG CTGTCTGCAC CCAGCTCTGC TGTTGCTGTG 
CCCAGGGCTG CCCAGGACCC GCCCTCCATG CCTGTCGGAG CATCGCCCGA AACCAGGGGG 
CCGAGGACCG AGGACGTCCC TGTGTCCTGC GCAAATTCCT GTGAAATGTG CCGAGAAGTA 
TTCAAGTCCA AACACGTGCG TGAGCTGCAG TGTGGGCACA GGCTTCACAA GGGGTGCTTC 
AAGCAGTGGC TGAAGAGCCA GCGCACCTGC CCAGCCTGCC TGGGTCACGA CCTCCTGTCA 
GAGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_010801678.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010799980.2 Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X9, mRNA.

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CloneID	OBa218658
Clone ID Related Accession (Same CDS sequence)	XM_010801678.3
Accession Version	XM_010801678.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5271bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TTC3 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010801678.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACGAA AAAAAGCATT CTTGATGAAG GTGAAAAACA TCTGTGGGTG GTGTACCATG 
GCCGATTTTC CCCAGGGAAA TTTTGCAGAT TATGCCTTGT TAGAAGATTG CCCTTATGTG 
GATGATTGTA TCTTTGCCCC TGAGTTTATG TCCAGTGATT ATGTTCGTGT GACTCAGCTT 
TACTGTGATG GAGTGGGTAG ACAGTATAAA GATTATGCCC AAACTGAGGG AAACTTAGAA 
TTTGACATCT GCACTCTGTG GTGTAGTCAA CCTATTTCTG TCCTGCAAGA TTACTGTGAT 
GCCATTAAAG TCCACATCTT CTGGCCACTT CTGTTTCAAC GTCAGCAGAG CTCTATAATA 
ACACGGATGC ACTCCTGTGT AAGGACCAGC GGTTCTCATG CTTGTGAGAT AAGTTTGAAG 
AAATTACAGC AGCTTGAACT GATGGAAGAT ATTGTGGATT TGGCAAAGAA AGTTGTGAAT 
GATACATTCT TTATCGGAGG CTTATTGAGA ATTGGCTATA AAATAGAGAA TAAAATCTTG 
GCAATGGAAG AAGCTTTGAA TTGGGTGAAA TACACAGGCG ATGTAACAAT TTTATCTAAA 
TTAGGAGCAG TTGATAATTG TTGGCGTATG TTAAGTATTT TCTTTACTGA ATACAAGTAT 
CACATAACTA AAGTTGTAAC AGAAAACTGC AGTTTGCTTG AAGAATTTAA AACTCAAAGT 
TGTGCGGAGT GTCTAGAGCA AGGAGAACTA ATGAAAATGA AAGGAAATGA AGAGTTTTCC 
AAAGAAAGAT ATGATATAGC TATTATCTAT TACACCAGAG CCATTGAATG TAGGCCTGAA 
AATCACCTGC TTTATGGTAA CCGAGCTCTT TGTTTTCTTC GTACTGGACA ATTCAGAAAT 
GCACTTGGTG ATGGAAAGAG AGCTACCATT CTGAAGAACA ATTGGACAAA GGGTCATTAT 
CGTTATTGTG CTGCTCTTTC TATGCTGGGG GAATATGACT GGGCCCTGCA AGCAAACATA 
AAAGCTCAAA AACTCTGTAA AAATGACCCT GAGGGAATCA AGGATCTAAT TCAGCAGCAT 
GTAAAGTTAC AAAAACAAAT AGAAGACCTG CAAGGTCAAA CACCAGCTAG TAATCCAGTC 
AAAGCCTTCT GTGAAAGCAG GGCCTACATA CCTAGTTTAT CAGCACCTGC ATTTAGTACT 
TCACTTAACT TTGTGGAAAC TGAAAAAAAT TTCAGAAAAA CTAACCACGA AATGGCAAAC 
GGTGGTAATC AGAATCCAAA GGTGGTAGAT GAAGCTCTGA AAGTAGATGA TTGTGACTGT 
CATCCTGAAT TTGTACCGCC ATCAAGTCAG CCTCCAAAAT ATAAAGGGAA ACAAAAATCT 
CGAAACAATG AATTGGAGAA GTTCAGCCCT GGTTCACAAG AAACTTTACC AGTAGATTTA 
AAAAACATCT TGGAGAAACA GTTTTCTAAA TCTTCCAGAG CCGCACACCA GGATTTTGCT 
AATATAATGA AAATGTTGAG AAGTTTAATT CAGGATGGCT ACACGGCCTT GCTGGAGCAG 
CGCTGCCGCA GCGCCGCCCA AGCCTTCACA GAGTTGCTCA ACGGTTTAGA TCCTCAAAAA 
ATAAAGCAAT TAAACCTGGC CATGATTAAC TACGTGTTAG TAGTCTACGG ACTTGCCATT 
TCACTCCTTG GAATAGGACA GCCCGAGGAG TTATCTGAAG CTGAAAACCA GTTCAAGAGG 
ATGATTGAAC ACTACCCCAA TGAGGGGCTT GATTGCTTGG CCTACTGTGG AATTGGAAAA 
GTGTATTTGA AAAAAAACAG ATTTTTAGAA GCTCTTAATC ATTTTGAGAA AGCAAGAACC 
TTGATCTGTC GTCTTCCTGG AGTGTTAACT TGGCCCACAA GTAATGTGGT TATTGAGGAG 
ACTCAGCCAG GGAAGATAAA GATGCTGTTA GAGAAATTTG TTGAAGAATG CAGGTTCCCT 
CCAGTGCCGG ATGCCACTTG TTGCTATCAG AAGTGCCATG GATATTCCAA AATCCAGATA 
TATATAACTG ATCCAGACTT TAAGGGTTTT ATACGGATCA GCTGTTGCCA GTATTGTAAA 
ATAGAATTTC ACATGAACTG CTGGAAGAAG TTAAAAACAA CAACCTTCAA TGATAAAATT 
GACAAGGATT TTCTGCAAGG AATCTGTCTT ACTCCTGATT GTGAAGGAAT CATTTCAAAG 
ATTATCATCT TCAGCAGTGG TGGTCAAGTT AAATGTGAAT TTGAACACAA GGTCATTAAA 
GAAAAGGTTC CTCCAAGACC TATTCTGAAA CAGAAATGTT CTAGCCTAGA GAAGTTGAGG 
CTGAAAGAAG ACAAAAAATT GAAGCGAAAG CTCCAAAAAA AGGAAGCAAA AAAATTAGCA 
CAAGAAAGAC TGGAAGAGGA CTTAAGGGAA AGTAACCCAC CAAAAACTGA AGAGCAGAGA 
GAAACTATCA ACCATGTCCA AAGTTGCCAG TTCCTTGATG ACAGAATTCT GCAGTGCATA 
AAGCAGTACG CTGACAAGAT TAAGTCCGGG ATACTGAATA CTTCCAAGCT TCTCAAAGAA 
TTGCTTTCTT GGAAGGTTTT AACCACAGAA GACTACACAA CGTGTTTTTC TAGCAGAAAT 
TTCCTGAATG AAGCAGTGGA CTATGTCATT CGTCATTTGA TCCAAGAAAA GAATAGAGTA 
AAGACAAGGG TATTTCTGCA TGTTTTGAGT GAGCTTAAAG AAGTGGACCC AAAACTAGCC 
ACCTGGATCC AGAAACTGAA TAGCTTTGGC TTAGATGCCA CAGGACCTTT CTTTTCTCGT 
TATGGGGCTC CTCTTAAGGA GCTTGATTTT AGCATCATGA CTTTTCTCTG GAATGAGAAA 
TACGGTCATA AGCTAGGCTC TATAGAAGGA AAGCAACTTG ATTATTTCTA TGAGCCTACA 
TCACTGAAGG AAGCGCGGTG TTTAATATGG CTGCTAGAAG AACACAGAGA CAAGTTCCCA 
GCACTGCATA ATGCCTTGGA CGAGTTCTTT GATATAATGG ACAGCCGCTG CACTGTATTA 
AGGAAACAGG ACAGTGGTGA CGTGCCATTT AATTCTACCA AGATAAAAAA CAAAGGCAAG 
AAAAAGAAGC CAAAAGATTC AAAGCCTATG TTAGTTGGAT CTGGAACACC TTCAGTCACA 
CCAAACAATG AGACTGTCAC TTCAGGTGAA GACCATATCA AACGCAGTTT AAGTTCTGCG 
GGCCCCTTTG TAGTGCCTGA TCACCTTCGG CAGGATATAG AAGAATTTGA AGCTCTGTAT 
GAACAGCATA GTAATGAGTA TGTTGTCCGT AATAAGAAGC TGTGGGACAT AAACCCAAAA 
CAGAAATGTT CAACTCTATA CGATTATTTC TCTCAGTTGT TGGAGGAACA TGGTCCCTTG 
GACATGAGTA ACAAAATGTT TTCTGAAGAA TATGAGTTCT TCCCAGAAGA AACTCAACAG 
ATACTAGAAA AAGCAGGAGG CTTGAAAGCG TTTCTCCTGG GATGTCCTCG TTTTGTTGTG 
ATTGATAACT GTATCGCATT GAAGAAAGTT GCATTACGGC TCAAGAAAAA GAGAAAGAAG 
AAAAACATTA AAACAAAAGT AGAAGAAATT TCAAAAACAG GAGAGTATTT ACGAGTTAAA 
CTACCACTGA ATCCAACTGC TAGGGAATTT AAACCAGATG TAAAGTCCAA ACCAGTGTCT 
GACTTACCTT CAGCACCAGC TTCTGAGGAC ATGAAGCCTC AGCCTGCACC TGCTAATTCT 
CCCCAATCAG CTCGTGACAC TGGGAAGCCA AAACCAGTTT TCGATAATTC TTCCAGATCA 
GTTTCTGAGG ATGAGAAGCC CAAAGGGATG TCTTCTGATT CTCCCAGGCC AGTCTTTGAG 
GATACCGGTC ACAAGCCGGT CTCCTCTAAT TCTCCCAAAC CAGTTCCTGA GGAAGTGAAA 
GCGACCTATT GGCCTCGACC CCAGTTGGTC ACAGGATACT GTACATATCT TCCCTTTCGG 
GGATTTGACA TTACCCAGAC TCCACCAGCC TTCATCAGTG TGTTACCGAC TTTGCCCCAG 
TATAGCATTT ACACCCCTCT GGCCAGTGTT CCTACTGAGT ATCACCTGCA AAGACCAATA 
CCAGTGGTGC CGTCTTTTGT AGCCAGTGAC ATAACGGAAG TGCATTTTGA GGCTCATCAT 
TTCAGTGCTG AGAATGCCCC TGGTACCCAG ATTGCCTCCG AAACACAGAT CCTTGAGGAA 
TCTTTGGAAA CACCAGTGAA GTCAGAGTGC AGCTATGATG GTGCTGATAT AGTCCAGAGT 
GAGGCTAGCA GAAACGATGA CTACTGTGGC GATTCTGCCA AATGGGAAGT AAATCCAGAA 
AGCACCAATG AAGTAACAAA TATTCCATGT ACACAAGTGG TTGCTGTACA GGTGTCTTGG 
AATATAACAC ATCAGGAAGT CAATACTGAG CCATATGGTC CTTTTGAGGC ACAACAGGGG 
GGTATTTCAC AGGTTGAAAA GGAGTACCAG GTTTTACAAG AGCAGCTTAA GGAAGCATGT 
GAAAATTATG AACACAGCAA TGTCAAGGGC TCAGAAGAAA CCAAGGACTT GGAAGAAAAG 
TTGAAAAGGA ATGTTGAAGA AAATAAGATC TCAAAGACAG AACTAGATTG GTTCCTTGAA 
GATTTGAACA AGGAAATTAA AAAATGGGAA CAGGAGAAAA AAGAAATCCA AGAAAGACTA 
AAGGCACTGA AGAAGAAAAT GAAAAAGGTG TTAAATGCCA GCAAAATGTA TACGCAGGAA 
AATGATGGAA AGGAAAAGGC ACTTGAATTA CTTCTGGATC AGTCCCTTGA AATCAGTAAT 
ACATTTACAA ATGAGAAAAT GAAAATAGAA GAGAGTATAA AGAAAGGGAA AGAGCATTAT 
GAAGAGAGTC TTCAAAGGGC TGTGGATGCA GAGGTATCTG TGCTTGAAAA CTGGAAGGAC 
ATGGAAGTGT GTAAGTTACA GAGCATGGAG TCACAAGCCG AAAGGTTTCT CAAGAATCTG 
AAGCTAATGA GCAGTGATTC GGCAGCGTAT TCTGACATGG ACTCAGACAT ACATTCATGG 
GAATCGTTTC TTTCTGATGT TAGAGAAGAA ATTGAGAAAG CAAAGTCTCA GTTTGAAGAA 
CAAATTCAGG CAATTAAATA TGGTTCTCAG CTCAGTGAAC TTCCTAAAGT GCAGATTTCT 
GAGCTTTCAT TTCCTACCTG TAACACGCAC GGAGCTTGCT GGTTTTATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010799980.2
CDS	2836..8106
Translation	MGRKKAFLMKVKNICGWCTMADFPQGNFADYALLEDCPYVDDCIFAPEFMSSDYVRVTQLYCDGVGRQYKDYAQTEGNLEFDICTLWCSQPISVLQDYCDAIKVHIFWPLLFQRQQSSIITRMHSCVRTSGSHACEISLKKLQQLELMEDIVDLAKKVVNDTFFIGGLLRIGYKIENKILAMEEALNWVKYTGDVTILSKLGAVDNCWRMLSIFFTEYKYHITKVVTENCSLLEEFKTQSCAECLEQGELMKMKGNEEFSKERYDIAIIYYTRAIECRPENHLLYGNRALCFLRTGQFRNALGDGKRATILKNNWTKGHYRYCAALSMLGEYDWALQANIKAQKLCKNDPEGIKDLIQQHVKLQKQIEDLQGQTPASNPVKAFCESRAYIPSLSAPAFSTSLNFVETEKNFRKTNHEMANGGNQNPKVVDEALKVDDCDCHPEFVPPSSQPPKYKGKQKSRNNELEKFSPGSQETLPVDLKNILEKQFSKSSRAAHQDFANIMKMLRSLIQDGYTALLEQRCRSAAQAFTELLNGLDPQKIKQLNLAMINYVLVVYGLAISLLGIGQPEELSEAENQFKRMIEHYPNEGLDCLAYCGIGKVYLKKNRFLEALNHFEKARTLICRLPGVLTWPTSNVVIEETQPGKIKMLLEKFVEECRFPPVPDATCCYQKCHGYSKIQIYITDPDFKGFIRISCCQYCKIEFHMNCWKKLKTTTFNDKIDKDFLQGICLTPDCEGIISKIIIFSSGGQVKCEFEHKVIKEKVPPRPILKQKCSSLEKLRLKEDKKLKRKLQKKEAKKLAQERLEEDLRESNPPKTEEQRETINHVQSCQFLDDRILQCIKQYADKIKSGILNTSKLLKELLSWKVLTTEDYTTCFSSRNFLNEAVDYVIRHLIQEKNRVKTRVFLHVLSELKEVDPKLATWIQKLNSFGLDATGPFFSRYGAPLKELDFSIMTFLWNEKYGHKLGSIEGKQLDYFYEPTSLKEARCLIWLLEEHRDKFPALHNALDEFFDIMDSRCTVLRKQDSGDVPFNSTKIKNKGKKKKPKDSKPMLVGSGTPSVTPNNETVTSGEDHIKRSLSSAGPFVVPDHLRQDIEEFEALYEQHSNEYVVRNKKLWDINPKQKCSTLYDYFSQLLEEHGPLDMSNKMFSEEYEFFPEETQQILEKAGGLKAFLLGCPRFVVIDNCIALKKVALRLKKKRKKKNIKTKVEEISKTGEYLRVKLPLNPTAREFKPDVKSKPVSDLPSAPASEDMKPQPAPANSPQSARDTGKPKPVFDNSSRSVSEDEKPKGMSSDSPRPVFEDTGHKPVSSNSPKPVPEEVKATYWPRPQLVTGYCTYLPFRGFDITQTPPAFISVLPTLPQYSIYTPLASVPTEYHLQRPIPVVPSFVASDITEVHFEAHHFSAENAPGTQIASETQILEESLETPVKSECSYDGADIVQSEASRNDDYCGDSAKWEVNPESTNEVTNIPCTQVVAVQVSWNITHQEVNTEPYGPFEAQQGGISQVEKEYQVLQEQLKEACENYEHSNVKGSEETKDLEEKLKRNVEENKISKTELDWFLEDLNKEIKKWEQEKKEIQERLKALKKKMKKVLNASKMYTQENDGKEKALELLLDQSLEISNTFTNEKMKIEESIKKGKEHYEESLQRAVDAEVSVLENWKDMEVCKLQSMESQAERFLKNLKLMSSDSAAYSDMDSDIHSWESFLSDVREEIEKAKSQFEEQIQAIKYGSQLSELPKVQISELSFPTCNTHGACWFY

Target ORF information:

RefSeq Version	XM_010801678.3
Organism	Bos taurus(cattle)
Definition	Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010801678.3

ORF Insert Sequence:

ATGGGACGAA AAAAAGCATT CTTGATGAAG GTGAAAAACA TCTGTGGGTG GTGTACCATG 
GCCGATTTTC CCCAGGGAAA TTTTGCAGAT TATGCCTTGT TAGAAGATTG CCCTTATGTG 
GATGATTGTA TCTTTGCCCC TGAGTTTATG TCCAGTGATT ATGTTCGTGT GACTCAGCTT 
TACTGTGATG GAGTGGGTAG ACAGTATAAA GATTATGCCC AAACTGAGGG AAACTTAGAA 
TTTGACATCT GCACTCTGTG GTGTAGTCAA CCTATTTCTG TCCTGCAAGA TTACTGTGAT 
GCCATTAAAG TCCACATCTT CTGGCCACTT CTGTTTCAAC GTCAGCAGAG CTCTATAATA 
ACACGGATGC ACTCCTGTGT AAGGACCAGC GGTTCTCATG CTTGTGAGAT AAGTTTGAAG 
AAATTACAGC AGCTTGAACT GATGGAAGAT ATTGTGGATT TGGCAAAGAA AGTTGTGAAT 
GATACATTCT TTATCGGAGG CTTATTGAGA ATTGGCTATA AAATAGAGAA TAAAATCTTG 
GCAATGGAAG AAGCTTTGAA TTGGGTGAAA TACACAGGCG ATGTAACAAT TTTATCTAAA 
TTAGGAGCAG TTGATAATTG TTGGCGTATG TTAAGTATTT TCTTTACTGA ATACAAGTAT 
CACATAACTA AAGTTGTAAC AGAAAACTGC AGTTTGCTTG AAGAATTTAA AACTCAAAGT 
TGTGCGGAGT GTCTAGAGCA AGGAGAACTA ATGAAAATGA AAGGAAATGA AGAGTTTTCC 
AAAGAAAGAT ATGATATAGC TATTATCTAT TACACCAGAG CCATTGAATG TAGGCCTGAA 
AATCACCTGC TTTATGGTAA CCGAGCTCTT TGTTTTCTTC GTACTGGACA ATTCAGAAAT 
GCACTTGGTG ATGGAAAGAG AGCTACCATT CTGAAGAACA ATTGGACAAA GGGTCATTAT 
CGTTATTGTG CTGCTCTTTC TATGCTGGGG GAATATGACT GGGCCCTGCA AGCAAACATA 
AAAGCTCAAA AACTCTGTAA AAATGACCCT GAGGGAATCA AGGATCTAAT TCAGCAGCAT 
GTAAAGTTAC AAAAACAAAT AGAAGACCTG CAAGGTCAAA CACCAGCTAG TAATCCAGTC 
AAAGCCTTCT GTGAAAGCAG GGCCTACATA CCTAGTTTAT CAGCACCTGC ATTTAGTACT 
TCACTTAACT TTGTGGAAAC TGAAAAAAAT TTCAGAAAAA CTAACCACGA AATGGCAAAC 
GGTGGTAATC AGAATCCAAA GGTGGTAGAT GAAGCTCTGA AAGTAGATGA TTGTGACTGT 
CATCCTGAAT TTGTACCGCC ATCAAGTCAG CCTCCAAAAT ATAAAGGGAA ACAAAAATCT 
CGAAACAATG AATTGGAGAA GTTCAGCCCT GGTTCACAAG AAACTTTACC AGTAGATTTA 
AAAAACATCT TGGAGAAACA GTTTTCTAAA TCTTCCAGAG CCGCACACCA GGATTTTGCT 
AATATAATGA AAATGTTGAG AAGTTTAATT CAGGATGGCT ACACGGCCTT GCTGGAGCAG 
CGCTGCCGCA GCGCCGCCCA AGCCTTCACA GAGTTGCTCA ACGGTTTAGA TCCTCAAAAA 
ATAAAGCAAT TAAACCTGGC CATGATTAAC TACGTGTTAG TAGTCTACGG ACTTGCCATT 
TCACTCCTTG GAATAGGACA GCCCGAGGAG TTATCTGAAG CTGAAAACCA GTTCAAGAGG 
ATGATTGAAC ACTACCCCAA TGAGGGGCTT GATTGCTTGG CCTACTGTGG AATTGGAAAA 
GTGTATTTGA AAAAAAACAG ATTTTTAGAA GCTCTTAATC ATTTTGAGAA AGCAAGAACC 
TTGATCTGTC GTCTTCCTGG AGTGTTAACT TGGCCCACAA GTAATGTGGT TATTGAGGAG 
ACTCAGCCAG GGAAGATAAA GATGCTGTTA GAGAAATTTG TTGAAGAATG CAGGTTCCCT 
CCAGTGCCGG ATGCCACTTG TTGCTATCAG AAGTGCCATG GATATTCCAA AATCCAGATA 
TATATAACTG ATCCAGACTT TAAGGGTTTT ATACGGATCA GCTGTTGCCA GTATTGTAAA 
ATAGAATTTC ACATGAACTG CTGGAAGAAG TTAAAAACAA CAACCTTCAA TGATAAAATT 
GACAAGGATT TTCTGCAAGG AATCTGTCTT ACTCCTGATT GTGAAGGAAT CATTTCAAAG 
ATTATCATCT TCAGCAGTGG TGGTCAAGTT AAATGTGAAT TTGAACACAA GGTCATTAAA 
GAAAAGGTTC CTCCAAGACC TATTCTGAAA CAGAAATGTT CTAGCCTAGA GAAGTTGAGG 
CTGAAAGAAG ACAAAAAATT GAAGCGAAAG CTCCAAAAAA AGGAAGCAAA AAAATTAGCA 
CAAGAAAGAC TGGAAGAGGA CTTAAGGGAA AGTAACCCAC CAAAAACTGA AGAGCAGAGA 
GAAACTATCA ACCATGTCCA AAGTTGCCAG TTCCTTGATG ACAGAATTCT GCAGTGCATA 
AAGCAGTACG CTGACAAGAT TAAGTCCGGG ATACTGAATA CTTCCAAGCT TCTCAAAGAA 
TTGCTTTCTT GGAAGGTTTT AACCACAGAA GACTACACAA CGTGTTTTTC TAGCAGAAAT 
TTCCTGAATG AAGCAGTGGA CTATGTCATT CGTCATTTGA TCCAAGAAAA GAATAGAGTA 
AAGACAAGGG TATTTCTGCA TGTTTTGAGT GAGCTTAAAG AAGTGGACCC AAAACTAGCC 
ACCTGGATCC AGAAACTGAA TAGCTTTGGC TTAGATGCCA CAGGACCTTT CTTTTCTCGT 
TATGGGGCTC CTCTTAAGGA GCTTGATTTT AGCATCATGA CTTTTCTCTG GAATGAGAAA 
TACGGTCATA AGCTAGGCTC TATAGAAGGA AAGCAACTTG ATTATTTCTA TGAGCCTACA 
TCACTGAAGG AAGCGCGGTG TTTAATATGG CTGCTAGAAG AACACAGAGA CAAGTTCCCA 
GCACTGCATA ATGCCTTGGA CGAGTTCTTT GATATAATGG ACAGCCGCTG CACTGTATTA 
AGGAAACAGG ACAGTGGTGA CGTGCCATTT AATTCTACCA AGATAAAAAA CAAAGGCAAG 
AAAAAGAAGC CAAAAGATTC AAAGCCTATG TTAGTTGGAT CTGGAACACC TTCAGTCACA 
CCAAACAATG AGACTGTCAC TTCAGGTGAA GACCATATCA AACGCAGTTT AAGTTCTGCG 
GGCCCCTTTG TAGTGCCTGA TCACCTTCGG CAGGATATAG AAGAATTTGA AGCTCTGTAT 
GAACAGCATA GTAATGAGTA TGTTGTCCGT AATAAGAAGC TGTGGGACAT AAACCCAAAA 
CAGAAATGTT CAACTCTATA CGATTATTTC TCTCAGTTGT TGGAGGAACA TGGTCCCTTG 
GACATGAGTA ACAAAATGTT TTCTGAAGAA TATGAGTTCT TCCCAGAAGA AACTCAACAG 
ATACTAGAAA AAGCAGGAGG CTTGAAAGCG TTTCTCCTGG GATGTCCTCG TTTTGTTGTG 
ATTGATAACT GTATCGCATT GAAGAAAGTT GCATTACGGC TCAAGAAAAA GAGAAAGAAG 
AAAAACATTA AAACAAAAGT AGAAGAAATT TCAAAAACAG GAGAGTATTT ACGAGTTAAA 
CTACCACTGA ATCCAACTGC TAGGGAATTT AAACCAGATG TAAAGTCCAA ACCAGTGTCT 
GACTTACCTT CAGCACCAGC TTCTGAGGAC ATGAAGCCTC AGCCTGCACC TGCTAATTCT 
CCCCAATCAG CTCGTGACAC TGGGAAGCCA AAACCAGTTT TCGATAATTC TTCCAGATCA 
GTTTCTGAGG ATGAGAAGCC CAAAGGGATG TCTTCTGATT CTCCCAGGCC AGTCTTTGAG 
GATACCGGTC ACAAGCCGGT CTCCTCTAAT TCTCCCAAAC CAGTTCCTGA GGAAGTGAAA 
GCGACCTATT GGCCTCGACC CCAGTTGGTC ACAGGATACT GTACATATCT TCCCTTTCGG 
GGATTTGACA TTACCCAGAC TCCACCAGCC TTCATCAGTG TGTTACCGAC TTTGCCCCAG 
TATAGCATTT ACACCCCTCT GGCCAGTGTT CCTACTGAGT ATCACCTGCA AAGACCAATA 
CCAGTGGTGC CGTCTTTTGT AGCCAGTGAC ATAACGGAAG TGCATTTTGA GGCTCATCAT 
TTCAGTGCTG AGAATGCCCC TGGTACCCAG ATTGCCTCCG AAACACAGAT CCTTGAGGAA 
TCTTTGGAAA CACCAGTGAA GTCAGAGTGC AGCTATGATG GTGCTGATAT AGTCCAGAGT 
GAGGCTAGCA GAAACGATGA CTACTGTGGC GATTCTGCCA AATGGGAAGT AAATCCAGAA 
AGCACCAATG AAGTAACAAA TATTCCATGT ACACAAGTGG TTGCTGTACA GGTGTCTTGG 
AATATAACAC ATCAGGAAGT CAATACTGAG CCATATGGTC CTTTTGAGGC ACAACAGGGG 
GGTATTTCAC AGGTTGAAAA GGAGTACCAG GTTTTACAAG AGCAGCTTAA GGAAGCATGT 
GAAAATTATG AACACAGCAA TGTCAAGGGC TCAGAAGAAA CCAAGGACTT GGAAGAAAAG 
TTGAAAAGGA ATGTTGAAGA AAATAAGATC TCAAAGACAG AACTAGATTG GTTCCTTGAA 
GATTTGAACA AGGAAATTAA AAAATGGGAA CAGGAGAAAA AAGAAATCCA AGAAAGACTA 
AAGGCACTGA AGAAGAAAAT GAAAAAGGTG TTAAATGCCA GCAAAATGTA TACGCAGGAA 
AATGATGGAA AGGAAAAGGC ACTTGAATTA CTTCTGGATC AGTCCCTTGA AATCAGTAAT 
ACATTTACAA ATGAGAAAAT GAAAATAGAA GAGAGTATAA AGAAAGGGAA AGAGCATTAT 
GAAGAGAGTC TTCAAAGGGC TGTGGATGCA GAGGTATCTG TGCTTGAAAA CTGGAAGGAC 
ATGGAAGTGT GTAAGTTACA GAGCATGGAG TCACAAGCCG AAAGGTTTCT CAAGAATCTG 
AAGCTAATGA GCAGTGATTC GGCAGCGTAT TCTGACATGG ACTCAGACAT ACATTCATGG 
GAATCGTTTC TTTCTGATGT TAGAGAAGAA ATTGAGAAAG CAAAGTCTCA GTTTGAAGAA 
CAAATTCAGG CAATTAAATA TGGTTCTCAG CTCAGTGAAC TTCCTAAAGT GCAGATTTCT 
GAGCTTTCAT TTCCTACCTG TAACACGCAC GGAGCTTGCT GGTTTTATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_010801679.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010799981.1 Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X10, mRNA.

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CloneID	OBa218659
Clone ID Related Accession (Same CDS sequence)	XM_010801679.3
Accession Version	XM_010801679.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5265bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TTC3 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010801679.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATGA AAGGAAATGA AGAGTTTTCC AAAGAAAGAT ATGATATAGC TATTATCTAT 
TACACCAGAG CCATTGAATG TAGGCCTGAA AATCACCTGC TTTATGGTAA CCGAGCTCTT 
TGTTTTCTTC GTACTGGACA ATTCAGAAAT GCACTTGGTG ATGGAAAGAG AGCTACCATT 
CTGAAGAACA ATTGGACAAA GGGTCATTAT CGTTATTGTG CTGCTCTTTC TATGCTGGGG 
GAATATGACT GGGCCCTGCA AGCAAACATA AAAGCTCAAA AACTCTGTAA AAATGACCCT 
GAGGGAATCA AGGATCTAAT TCAGCAGCAT GTAAAGTTAC AAAAACAAAT AGAAGACCTG 
CAAGGTCAAA CACCAGCTAG TAATCCAGTC AAAGCCTTCT GTGAAAGCAG GGCCTACATA 
CCTAGTTTAT CAGCACCTGC ATTTAGTACT TCACTTAACT TTGTGGAAAC TGAAAAAAAT 
TTCAGAAAAA CTAACCACGA AATGGCAAAC GGTGGTAATC AGAATCCAAA GGTGGTAGAT 
GAAGCTCTGA AAGTAGATGA TTGTGACTGT CATCCTGAAT TTGTACCGCC ATCAAGTCAG 
CCTCCAAAAT ATAAAGGGAA ACAAAAATCT CGAAACAATG AATTGGAGAA GTTCAGCCCT 
GGTTCACAAG AAACTTTACC AGTAGATTTA AAAAACATCT TGGAGAAACA GTTTTCTAAA 
TCTTCCAGAG CCGCACACCA GGATTTTGCT AATATAATGA AAATGTTGAG AAGTTTAATT 
CAGGATGGCT ACACGGCCTT GCTGGAGCAG CGCTGCCGCA GCGCCGCCCA AGCCTTCACA 
GAGTTGCTCA ACGGTTTAGA TCCTCAAAAA ATAAAGCAAT TAAACCTGGC CATGATTAAC 
TACGTGTTAG TAGTCTACGG ACTTGCCATT TCACTCCTTG GAATAGGACA GCCCGAGGAG 
TTATCTGAAG CTGAAAACCA GTTCAAGAGG ATGATTGAAC ACTACCCCAA TGAGGGGCTT 
GATTGCTTGG CCTACTGTGG AATTGGAAAA GTGTATTTGA AAAAAAACAG ATTTTTAGAA 
GCTCTTAATC ATTTTGAGAA AGCAAGAACC TTGATCTGTC GTCTTCCTGG AGTGTTAACT 
TGGCCCACAA GTAATGTGGT TATTGAGGAG ACTCAGCCAG GGAAGATAAA GATGCTGTTA 
GAGAAATTTG TTGAAGAATG CAGGTTCCCT CCAGTGCCGG ATGCCACTTG TTGCTATCAG 
AAGTGCCATG GATATTCCAA AATCCAGATA TATATAACTG ATCCAGACTT TAAGGGTTTT 
ATACGGATCA GCTGTTGCCA GTATTGTAAA ATAGAATTTC ACATGAACTG CTGGAAGAAG 
TTAAAAACAA CAACCTTCAA TGATAAAATT GACAAGGATT TTCTGCAAGG AATCTGTCTT 
ACTCCTGATT GTGAAGGAAT CATTTCAAAG ATTATCATCT TCAGCAGTGG TGGTCAAGTT 
AAATGTGAAT TTGAACACAA GGTCATTAAA GAAAAGGTTC CTCCAAGACC TATTCTGAAA 
CAGAAATGTT CTAGCCTAGA GAAGTTGAGG CTGAAAGAAG ACAAAAAATT GAAGCGAAAG 
CTCCAAAAAA AGGAAGCAAA AAAATTAGCA CAAGAAAGAC TGGAAGAGGA CTTAAGGGAA 
AGTAACCCAC CAAAAACTGA AGAGCAGAGA GAAACTATCA ACCATGTCCA AAGTTGCCAG 
TTCCTTGATG ACAGAATTCT GCAGTGCATA AAGCAGTACG CTGACAAGAT TAAGTCCGGG 
ATACTGAATA CTTCCAAGCT TCTCAAAGAA TTGCTTTCTT GGAAGGTTTT AACCACAGAA 
GACTACACAA CGTGTTTTTC TAGCAGAAAT TTCCTGAATG AAGCAGTGGA CTATGTCATT 
CGTCATTTGA TCCAAGAAAA GAATAGAGTA AAGACAAGGG TATTTCTGCA TGTTTTGAGT 
GAGCTTAAAG AAGTGGACCC AAAACTAGCC ACCTGGATCC AGAAACTGAA TAGCTTTGGC 
TTAGATGCCA CAGGACCTTT CTTTTCTCGT TATGGGGCTC CTCTTAAGGA GCTTGATTTT 
AGCATCATGA CTTTTCTCTG GAATGAGAAA TACGGTCATA AGCTAGGCTC TATAGAAGGA 
AAGCAACTTG ATTATTTCTA TGAGCCTACA TCACTGAAGG AAGCGCGGTG TTTAATATGG 
CTGCTAGAAG AACACAGAGA CAAGTTCCCA GCACTGCATA ATGCCTTGGA CGAGTTCTTT 
GATATAATGG ACAGCCGCTG CACTGTATTA AGGAAACAGG ACAGTGGTGA CGTGCCATTT 
AATTCTACCA AGATAAAAAA CAAAGGCAAG AAAAAGAAGC CAAAAGATTC AAAGCCTATG 
TTAGTTGGAT CTGGAACACC TTCAGTCACA CCAAACAATG AGACTGTCAC TTCAGGTGAA 
GACCATATCA AACGCAGTTT AAGTTCTGCG GGCCCCTTTG TAGTGCCTGA TCACCTTCGG 
CAGGATATAG AAGAATTTGA AGCTCTGTAT GAACAGCATA GTAATGAGTA TGTTGTCCGT 
AATAAGAAGC TGTGGGACAT AAACCCAAAA CAGAAATGTT CAACTCTATA CGATTATTTC 
TCTCAGTTGT TGGAGGAACA TGGTCCCTTG GACATGAGTA ACAAAATGTT TTCTGAAGAA 
TATGAGTTCT TCCCAGAAGA AACTCAACAG ATACTAGAAA AAGCAGGAGG CTTGAAAGCG 
TTTCTCCTGG GATGTCCTCG TTTTGTTGTG ATTGATAACT GTATCGCATT GAAGAAAGTT 
GCATTACGGC TCAAGAAAAA GAGAAAGAAG AAAAACATTA AAACAAAAGT AGAAGAAATT 
TCAAAAACAG GAGAGTATTT ACGAGTTAAA CTACCACTGA ATCCAACTGC TAGGGAATTT 
AAACCAGATG TAAAGTCCAA ACCAGTGTCT GACTTACCTT CAGCACCAGC TTCTGAGGAC 
ATGAAGCCTC AGCCTGCACC TGCTAATTCT CCCCAATCAG CTCGTGACAC TGGGAAGCCA 
AAACCAGTTT TCGATAATTC TTCCAGATCA GTTTCTGAGG ATGAGAAGCC CAAAGGGATG 
TCTTCTGATT CTCCCAGGCC AGTCTTTGAG GATACCGGTC ACAAGCCGGT CTCCTCTAAT 
TCTCCCAAAC CAGTTCCTGA GGAAGTGAAA GCGACCTATT GGCCTCGACC CCAGTTGGTC 
ACAGGATACT GTACATATCT TCCCTTTCGG GGATTTGACA TTACCCAGAC TCCACCAGCC 
TTCATCAGTG TGTTACCGAC TTTGCCCCAG TATAGCATTT ACACCCCTCT GGCCAGTGTT 
CCTACTGAGT ATCACCTGCA AAGACCAATA CCAGTGGTGC CGTCTTTTGT AGCCAGTGAC 
ATAACGGAAG TGCATTTTGA GGCTCATCAT TTCAGTGCTG AGAATGCCCC TGGTACCCAG 
ATTGCCTCCG AAACACAGAT CCTTGAGGAA TCTTTGGAAA CACCAGTGAA GTCAGAGTGC 
AGCTATGATG GTGCTGATAT AGTCCAGAGT GAGGCTAGCA GAAACGATGA CTACTGTGGC 
GATTCTGCCA AATGGGAAGT AAATCCAGAA AGCACCAATG AAGTAACAAA TATTCCATGT 
ACACAAGTGG TTGCTGTACA GGTGTCTTGG AATATAACAC ATCAGGAAGT CAATACTGAG 
CCATATGGTC CTTTTGAGGC ACAACAGGGG GGTATTTCAC AGGTTGAAAA GGAGTACCAG 
GTTTTACAAG AGCAGCTTAA GGAAGCATGT GAAAATTATG AACACAGCAA TGTCAAGGGC 
TCAGAAGAAA CCAAGGACTT GGAAGAAAAG TTGAAAAGGA ATGTTGAAGA AAATAAGATC 
TCAAAGACAG AACTAGATTG GTTCCTTGAA GATTTGAACA AGGAAATTAA AAAATGGGAA 
CAGGAGAAAA AAGAAATCCA AGAAAGACTA AAGGCACTGA AGAAGAAAAT GAAAAAGGTG 
TTAAATGCCA GCAAAATGTA TACGCAGGAA AATGATGGAA AGGAAAAGGC ACTTGAATTA 
CTTCTGGATC AGTCCCTTGA AATCAGTAAT ACATTTACAA ATGAGAAAAT GAAAATAGAA 
GAGAGTATAA AGAAAGGGAA AGAGCATTAT GAAGAGAGTC TTCAAAGGGC TGTGGATGCA 
GAGGTATCTG TGCTTGAAAA CTGGAAGGAC ATGGAAGTGT GTAAGTTACA GAGCATGGAG 
TCACAAGCCG AAAGGTTTCT CAAGAATCTG AAGCTAATGA GCAGTGATTC GGCAGCGTAT 
TCTGACATGG ACTCAGACAT ACATTCATGG GAATCGTTTC TTTCTGATGT TAGAGAAGAA 
ATTGAGAAAG CAAAGTCTCA GTTTGAAGAA CAAATTCAGG CAATTAAATA TGGTTCTCAG 
CTCAGTGAAC TTCCTAAAGT GCAGATTTCT GAGCTTTCAT TTCCTACCTG TAACACGATT 
CATCCTAAAT TACTTCCTGA GTCTTCAGGC CAGGATGGTC AAGGTCCCTC CACTTCTACA 
AGTCATGGGA CCGGAGACCA GACAGCAGCT CCCGAGGAGT CAAGTCTGGG CTCTGCAGCT 
GACAGCCCAG TGGAGTTGCC CTCCTGCAGT CAGAAAGCCC CCCAGCTGCC AGAGCATGAG 
GGTGCTGGCC AGGCAACTGG GCCAGAACCA GGCCCAGTGG CTGATCAGAA GTTACCTGCT 
GCTCCAGAGC GTGCCACTCA TTCAAGCCAG TCTCCAAAGA AGCCGTTCAA TAGCATTATT 
GAGCACCTGT TGGTGGTGTT CCCGTGTTAC AGCAGCACGG AGCTTGCTGG TTTTATTAAA 
AAAGTTAGAA ACAAAAACAA GACCTCTCTG TCTGGATTGA GTATTGACGA AATTGTGCAA 
AGAGTGACAG AACACGTCCT TGATGAAGAA AGAAAGAAAA AGCCAAGTCC AGGAAAGGGC 
TGGAGGCTGT CTGCACCCAG CTCTGCTGTT GCTGTGCCCA GGGCTGCCCA GGACCCGCCC 
TCCATGCCTG TCGGAGCATC GCCCGAAACC AGGGGGCCGA GGACCGAGGA CGTCCCTGTG 
TCCTGCGCAA ATTCCTGTGA AATGTGCCGA GAAGTATTCA AGTCCAAACA CGTGCGTGAG 
CTGCAGTGTG GGCACAGGCT TCACAAGGGG TGCTTCAAGC AGTGGCTGAA GAGCCAGCGC 
ACCTGCCCAG CCTGCCTGGG TCACGACCTC CTGTCAGAGG AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010799981.1
CDS	134..5398
Translation	MKMKGNEEFSKERYDIAIIYYTRAIECRPENHLLYGNRALCFLRTGQFRNALGDGKRATILKNNWTKGHYRYCAALSMLGEYDWALQANIKAQKLCKNDPEGIKDLIQQHVKLQKQIEDLQGQTPASNPVKAFCESRAYIPSLSAPAFSTSLNFVETEKNFRKTNHEMANGGNQNPKVVDEALKVDDCDCHPEFVPPSSQPPKYKGKQKSRNNELEKFSPGSQETLPVDLKNILEKQFSKSSRAAHQDFANIMKMLRSLIQDGYTALLEQRCRSAAQAFTELLNGLDPQKIKQLNLAMINYVLVVYGLAISLLGIGQPEELSEAENQFKRMIEHYPNEGLDCLAYCGIGKVYLKKNRFLEALNHFEKARTLICRLPGVLTWPTSNVVIEETQPGKIKMLLEKFVEECRFPPVPDATCCYQKCHGYSKIQIYITDPDFKGFIRISCCQYCKIEFHMNCWKKLKTTTFNDKIDKDFLQGICLTPDCEGIISKIIIFSSGGQVKCEFEHKVIKEKVPPRPILKQKCSSLEKLRLKEDKKLKRKLQKKEAKKLAQERLEEDLRESNPPKTEEQRETINHVQSCQFLDDRILQCIKQYADKIKSGILNTSKLLKELLSWKVLTTEDYTTCFSSRNFLNEAVDYVIRHLIQEKNRVKTRVFLHVLSELKEVDPKLATWIQKLNSFGLDATGPFFSRYGAPLKELDFSIMTFLWNEKYGHKLGSIEGKQLDYFYEPTSLKEARCLIWLLEEHRDKFPALHNALDEFFDIMDSRCTVLRKQDSGDVPFNSTKIKNKGKKKKPKDSKPMLVGSGTPSVTPNNETVTSGEDHIKRSLSSAGPFVVPDHLRQDIEEFEALYEQHSNEYVVRNKKLWDINPKQKCSTLYDYFSQLLEEHGPLDMSNKMFSEEYEFFPEETQQILEKAGGLKAFLLGCPRFVVIDNCIALKKVALRLKKKRKKKNIKTKVEEISKTGEYLRVKLPLNPTAREFKPDVKSKPVSDLPSAPASEDMKPQPAPANSPQSARDTGKPKPVFDNSSRSVSEDEKPKGMSSDSPRPVFEDTGHKPVSSNSPKPVPEEVKATYWPRPQLVTGYCTYLPFRGFDITQTPPAFISVLPTLPQYSIYTPLASVPTEYHLQRPIPVVPSFVASDITEVHFEAHHFSAENAPGTQIASETQILEESLETPVKSECSYDGADIVQSEASRNDDYCGDSAKWEVNPESTNEVTNIPCTQVVAVQVSWNITHQEVNTEPYGPFEAQQGGISQVEKEYQVLQEQLKEACENYEHSNVKGSEETKDLEEKLKRNVEENKISKTELDWFLEDLNKEIKKWEQEKKEIQERLKALKKKMKKVLNASKMYTQENDGKEKALELLLDQSLEISNTFTNEKMKIEESIKKGKEHYEESLQRAVDAEVSVLENWKDMEVCKLQSMESQAERFLKNLKLMSSDSAAYSDMDSDIHSWESFLSDVREEIEKAKSQFEEQIQAIKYGSQLSELPKVQISELSFPTCNTIHPKLLPESSGQDGQGPSTSTSHGTGDQTAAPEESSLGSAADSPVELPSCSQKAPQLPEHEGAGQATGPEPGPVADQKLPAAPERATHSSQSPKKPFNSIIEHLLVVFPCYSSTELAGFIKKVRNKNKTSLSGLSIDEIVQRVTEHVLDEERKKKPSPGKGWRLSAPSSAVAVPRAAQDPPSMPVGASPETRGPRTEDVPVSCANSCEMCREVFKSKHVRELQCGHRLHKGCFKQWLKSQRTCPACLGHDLLSEE

Target ORF information:

RefSeq Version	XM_010801679.3
Organism	Bos taurus(cattle)
Definition	Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010801679.3

ORF Insert Sequence:

ATGAAAATGA AAGGAAATGA AGAGTTTTCC AAAGAAAGAT ATGATATAGC TATTATCTAT 
TACACCAGAG CCATTGAATG TAGGCCTGAA AATCACCTGC TTTATGGTAA CCGAGCTCTT 
TGTTTTCTTC GTACTGGACA ATTCAGAAAT GCACTTGGTG ATGGAAAGAG AGCTACCATT 
CTGAAGAACA ATTGGACAAA GGGTCATTAT CGTTATTGTG CTGCTCTTTC TATGCTGGGG 
GAATATGACT GGGCCCTGCA AGCAAACATA AAAGCTCAAA AACTCTGTAA AAATGACCCT 
GAGGGAATCA AGGATCTAAT TCAGCAGCAT GTAAAGTTAC AAAAACAAAT AGAAGACCTG 
CAAGGTCAAA CACCAGCTAG TAATCCAGTC AAAGCCTTCT GTGAAAGCAG GGCCTACATA 
CCTAGTTTAT CAGCACCTGC ATTTAGTACT TCACTTAACT TTGTGGAAAC TGAAAAAAAT 
TTCAGAAAAA CTAACCACGA AATGGCAAAC GGTGGTAATC AGAATCCAAA GGTGGTAGAT 
GAAGCTCTGA AAGTAGATGA TTGTGACTGT CATCCTGAAT TTGTACCGCC ATCAAGTCAG 
CCTCCAAAAT ATAAAGGGAA ACAAAAATCT CGAAACAATG AATTGGAGAA GTTCAGCCCT 
GGTTCACAAG AAACTTTACC AGTAGATTTA AAAAACATCT TGGAGAAACA GTTTTCTAAA 
TCTTCCAGAG CCGCACACCA GGATTTTGCT AATATAATGA AAATGTTGAG AAGTTTAATT 
CAGGATGGCT ACACGGCCTT GCTGGAGCAG CGCTGCCGCA GCGCCGCCCA AGCCTTCACA 
GAGTTGCTCA ACGGTTTAGA TCCTCAAAAA ATAAAGCAAT TAAACCTGGC CATGATTAAC 
TACGTGTTAG TAGTCTACGG ACTTGCCATT TCACTCCTTG GAATAGGACA GCCCGAGGAG 
TTATCTGAAG CTGAAAACCA GTTCAAGAGG ATGATTGAAC ACTACCCCAA TGAGGGGCTT 
GATTGCTTGG CCTACTGTGG AATTGGAAAA GTGTATTTGA AAAAAAACAG ATTTTTAGAA 
GCTCTTAATC ATTTTGAGAA AGCAAGAACC TTGATCTGTC GTCTTCCTGG AGTGTTAACT 
TGGCCCACAA GTAATGTGGT TATTGAGGAG ACTCAGCCAG GGAAGATAAA GATGCTGTTA 
GAGAAATTTG TTGAAGAATG CAGGTTCCCT CCAGTGCCGG ATGCCACTTG TTGCTATCAG 
AAGTGCCATG GATATTCCAA AATCCAGATA TATATAACTG ATCCAGACTT TAAGGGTTTT 
ATACGGATCA GCTGTTGCCA GTATTGTAAA ATAGAATTTC ACATGAACTG CTGGAAGAAG 
TTAAAAACAA CAACCTTCAA TGATAAAATT GACAAGGATT TTCTGCAAGG AATCTGTCTT 
ACTCCTGATT GTGAAGGAAT CATTTCAAAG ATTATCATCT TCAGCAGTGG TGGTCAAGTT 
AAATGTGAAT TTGAACACAA GGTCATTAAA GAAAAGGTTC CTCCAAGACC TATTCTGAAA 
CAGAAATGTT CTAGCCTAGA GAAGTTGAGG CTGAAAGAAG ACAAAAAATT GAAGCGAAAG 
CTCCAAAAAA AGGAAGCAAA AAAATTAGCA CAAGAAAGAC TGGAAGAGGA CTTAAGGGAA 
AGTAACCCAC CAAAAACTGA AGAGCAGAGA GAAACTATCA ACCATGTCCA AAGTTGCCAG 
TTCCTTGATG ACAGAATTCT GCAGTGCATA AAGCAGTACG CTGACAAGAT TAAGTCCGGG 
ATACTGAATA CTTCCAAGCT TCTCAAAGAA TTGCTTTCTT GGAAGGTTTT AACCACAGAA 
GACTACACAA CGTGTTTTTC TAGCAGAAAT TTCCTGAATG AAGCAGTGGA CTATGTCATT 
CGTCATTTGA TCCAAGAAAA GAATAGAGTA AAGACAAGGG TATTTCTGCA TGTTTTGAGT 
GAGCTTAAAG AAGTGGACCC AAAACTAGCC ACCTGGATCC AGAAACTGAA TAGCTTTGGC 
TTAGATGCCA CAGGACCTTT CTTTTCTCGT TATGGGGCTC CTCTTAAGGA GCTTGATTTT 
AGCATCATGA CTTTTCTCTG GAATGAGAAA TACGGTCATA AGCTAGGCTC TATAGAAGGA 
AAGCAACTTG ATTATTTCTA TGAGCCTACA TCACTGAAGG AAGCGCGGTG TTTAATATGG 
CTGCTAGAAG AACACAGAGA CAAGTTCCCA GCACTGCATA ATGCCTTGGA CGAGTTCTTT 
GATATAATGG ACAGCCGCTG CACTGTATTA AGGAAACAGG ACAGTGGTGA CGTGCCATTT 
AATTCTACCA AGATAAAAAA CAAAGGCAAG AAAAAGAAGC CAAAAGATTC AAAGCCTATG 
TTAGTTGGAT CTGGAACACC TTCAGTCACA CCAAACAATG AGACTGTCAC TTCAGGTGAA 
GACCATATCA AACGCAGTTT AAGTTCTGCG GGCCCCTTTG TAGTGCCTGA TCACCTTCGG 
CAGGATATAG AAGAATTTGA AGCTCTGTAT GAACAGCATA GTAATGAGTA TGTTGTCCGT 
AATAAGAAGC TGTGGGACAT AAACCCAAAA CAGAAATGTT CAACTCTATA CGATTATTTC 
TCTCAGTTGT TGGAGGAACA TGGTCCCTTG GACATGAGTA ACAAAATGTT TTCTGAAGAA 
TATGAGTTCT TCCCAGAAGA AACTCAACAG ATACTAGAAA AAGCAGGAGG CTTGAAAGCG 
TTTCTCCTGG GATGTCCTCG TTTTGTTGTG ATTGATAACT GTATCGCATT GAAGAAAGTT 
GCATTACGGC TCAAGAAAAA GAGAAAGAAG AAAAACATTA AAACAAAAGT AGAAGAAATT 
TCAAAAACAG GAGAGTATTT ACGAGTTAAA CTACCACTGA ATCCAACTGC TAGGGAATTT 
AAACCAGATG TAAAGTCCAA ACCAGTGTCT GACTTACCTT CAGCACCAGC TTCTGAGGAC 
ATGAAGCCTC AGCCTGCACC TGCTAATTCT CCCCAATCAG CTCGTGACAC TGGGAAGCCA 
AAACCAGTTT TCGATAATTC TTCCAGATCA GTTTCTGAGG ATGAGAAGCC CAAAGGGATG 
TCTTCTGATT CTCCCAGGCC AGTCTTTGAG GATACCGGTC ACAAGCCGGT CTCCTCTAAT 
TCTCCCAAAC CAGTTCCTGA GGAAGTGAAA GCGACCTATT GGCCTCGACC CCAGTTGGTC 
ACAGGATACT GTACATATCT TCCCTTTCGG GGATTTGACA TTACCCAGAC TCCACCAGCC 
TTCATCAGTG TGTTACCGAC TTTGCCCCAG TATAGCATTT ACACCCCTCT GGCCAGTGTT 
CCTACTGAGT ATCACCTGCA AAGACCAATA CCAGTGGTGC CGTCTTTTGT AGCCAGTGAC 
ATAACGGAAG TGCATTTTGA GGCTCATCAT TTCAGTGCTG AGAATGCCCC TGGTACCCAG 
ATTGCCTCCG AAACACAGAT CCTTGAGGAA TCTTTGGAAA CACCAGTGAA GTCAGAGTGC 
AGCTATGATG GTGCTGATAT AGTCCAGAGT GAGGCTAGCA GAAACGATGA CTACTGTGGC 
GATTCTGCCA AATGGGAAGT AAATCCAGAA AGCACCAATG AAGTAACAAA TATTCCATGT 
ACACAAGTGG TTGCTGTACA GGTGTCTTGG AATATAACAC ATCAGGAAGT CAATACTGAG 
CCATATGGTC CTTTTGAGGC ACAACAGGGG GGTATTTCAC AGGTTGAAAA GGAGTACCAG 
GTTTTACAAG AGCAGCTTAA GGAAGCATGT GAAAATTATG AACACAGCAA TGTCAAGGGC 
TCAGAAGAAA CCAAGGACTT GGAAGAAAAG TTGAAAAGGA ATGTTGAAGA AAATAAGATC 
TCAAAGACAG AACTAGATTG GTTCCTTGAA GATTTGAACA AGGAAATTAA AAAATGGGAA 
CAGGAGAAAA AAGAAATCCA AGAAAGACTA AAGGCACTGA AGAAGAAAAT GAAAAAGGTG 
TTAAATGCCA GCAAAATGTA TACGCAGGAA AATGATGGAA AGGAAAAGGC ACTTGAATTA 
CTTCTGGATC AGTCCCTTGA AATCAGTAAT ACATTTACAA ATGAGAAAAT GAAAATAGAA 
GAGAGTATAA AGAAAGGGAA AGAGCATTAT GAAGAGAGTC TTCAAAGGGC TGTGGATGCA 
GAGGTATCTG TGCTTGAAAA CTGGAAGGAC ATGGAAGTGT GTAAGTTACA GAGCATGGAG 
TCACAAGCCG AAAGGTTTCT CAAGAATCTG AAGCTAATGA GCAGTGATTC GGCAGCGTAT 
TCTGACATGG ACTCAGACAT ACATTCATGG GAATCGTTTC TTTCTGATGT TAGAGAAGAA 
ATTGAGAAAG CAAAGTCTCA GTTTGAAGAA CAAATTCAGG CAATTAAATA TGGTTCTCAG 
CTCAGTGAAC TTCCTAAAGT GCAGATTTCT GAGCTTTCAT TTCCTACCTG TAACACGATT 
CATCCTAAAT TACTTCCTGA GTCTTCAGGC CAGGATGGTC AAGGTCCCTC CACTTCTACA 
AGTCATGGGA CCGGAGACCA GACAGCAGCT CCCGAGGAGT CAAGTCTGGG CTCTGCAGCT 
GACAGCCCAG TGGAGTTGCC CTCCTGCAGT CAGAAAGCCC CCCAGCTGCC AGAGCATGAG 
GGTGCTGGCC AGGCAACTGG GCCAGAACCA GGCCCAGTGG CTGATCAGAA GTTACCTGCT 
GCTCCAGAGC GTGCCACTCA TTCAAGCCAG TCTCCAAAGA AGCCGTTCAA TAGCATTATT 
GAGCACCTGT TGGTGGTGTT CCCGTGTTAC AGCAGCACGG AGCTTGCTGG TTTTATTAAA 
AAAGTTAGAA ACAAAAACAA GACCTCTCTG TCTGGATTGA GTATTGACGA AATTGTGCAA 
AGAGTGACAG AACACGTCCT TGATGAAGAA AGAAAGAAAA AGCCAAGTCC AGGAAAGGGC 
TGGAGGCTGT CTGCACCCAG CTCTGCTGTT GCTGTGCCCA GGGCTGCCCA GGACCCGCCC 
TCCATGCCTG TCGGAGCATC GCCCGAAACC AGGGGGCCGA GGACCGAGGA CGTCCCTGTG 
TCCTGCGCAA ATTCCTGTGA AATGTGCCGA GAAGTATTCA AGTCCAAACA CGTGCGTGAG 
CTGCAGTGTG GGCACAGGCT TCACAAGGGG TGCTTCAAGC AGTGGCTGAA GAGCCAGCGC 
ACCTGCCCAG CCTGCCTGGG TCACGACCTC CTGTCAGAGG AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_024999925.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855693.1 Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X11, mRNA.

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CloneID	OBa218660
Clone ID Related Accession (Same CDS sequence)	XM_024999925.1
Accession Version	XM_024999925.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TTC3 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGAAA ATCACCTGCT TTATGGTAAC CGAGCTCTTT GTTTTCTTCG TACTGGACAA 
TTCAGAAATG CACTTGGTGA TGGAAAGAGA GCTACCATTC TGAAGAACAA TTGGACAAAG 
GGTCATTATC GTTATTGTGC TGCTCTTTCT ATGCTGGGGG AATATGACTG GGCCCTGCAA 
GCAAACATAA AAGCTCAAAA ACTCTGTAAA AATGACCCTG AGGGAATCAA GGATCTAATT 
CAGCAGCATG TAAAGTTACA AAAACAAATA GAAGACCTGC AAGGTCAAAC ACCAGCTAGT 
AATCCAGTCA AAGCCTTCTG TGAAAGCAGG GCCTACATAC CTAGTTTATC AGCACCTGCA 
TTTAGTACTT CACTTAACTT TGTGGAAACT GAAAAAAATT TCAGAAAAAC TAACCACGAA 
ATGGCAAACG GTGGTAATCA GAATCCAAAG GTGGTAGATG AAGCTCTGAA AGTAGATGAT 
TGTGACTGTC ATCCTGAATT TGTACCGCCA TCAAGTCAGC CTCCAAAATA TAAAGGGAAA 
CAAAAATCTC GAAACAATGA ATTGGAGAAG TTCAGCCCTG GTTCACAAGA AACTTTACCA 
GTAGATTTAA AAAACATCTT GGAGAAACAG TTTTCTAAAT CTTCCAGAGC CGCACACCAG 
GATTTTGCTA ATATAATGAA AATGTTGAGA AGTTTAATTC AGGATGGCTA CACGGCCTTG 
CTGGAGCAGC GCTGCCGCAG CGCCGCCCAA GCCTTCACAG AGTTGCTCAA CGGTTTAGAT 
CCTCAAAAAA TAAAGCAATT AAACCTGGCC ATGATTAACT ACGTGTTAGT AGTCTACGGA 
CTTGCCATTT CACTCCTTGG AATAGGACAG CCCGAGGAGT TATCTGAAGC TGAAAACCAG 
TTCAAGAGGA TGATTGAACA CTACCCCAAT GAGGGGCTTG ATTGCTTGGC CTACTGTGGA 
ATTGGAAAAG TGTATTTGAA AAAAAACAGA TTTTTAGAAG CTCTTAATCA TTTTGAGAAA 
GCAAGAACCT TGATCTGTCG TCTTCCTGGA GTGTTAACTT GGCCCACAAG TAATGTGGTT 
ATTGAGGAGA CTCAGCCAGG GAAGATAAAG ATGCTGTTAG AGAAATTTGT TGAAGAATGC 
AGGTTCCCTC CAGTGCCGGA TGCCACTTGT TGCTATCAGA AGTGCCATGG ATATTCCAAA 
ATCCAGATAT ATATAACTGA TCCAGACTTT AAGGGTTTTA TACGGATCAG CTGTTGCCAG 
TATTGTAAAA TAGAATTTCA CATGAACTGC TGGAAGAAGT TAAAAACAAC AACCTTCAAT 
GATAAAATTG ACAAGGATTT TCTGCAAGGA ATCTGTCTTA CTCCTGATTG TGAAGGAATC 
ATTTCAAAGA TTATCATCTT CAGCAGTGGT GGTCAAGTTA AATGTGAATT TGAACACAAG 
GTCATTAAAG AAAAGGTTCC TCCAAGACCT ATTCTGAAAC AGAAATGTTC TAGCCTAGAG 
AAGTTGAGGC TGAAAGAAGA CAAAAAATTG AAGCGAAAGC TCCAAAAAAA GGAAGCAAAA 
AAATTAGCAC AAGAAAGACT GGAAGAGGAC TTAAGGGAAA GTAACCCACC AAAAACTGAA 
GAGCAGAGAG AAACTATCAA CCATGTCCAA AGTTGCCAGT TCCTTGATGA CAGAATTCTG 
CAGTGCATAA AGCAGTACGC TGACAAGATT AAGTCCGGGA TACTGAATAC TTCCAAGCTT 
CTCAAAGAAT TGCTTTCTTG GAAGGTTTTA ACCACAGAAG ACTACACAAC GTGTTTTTCT 
AGCAGAAATT TCCTGAATGA AGCAGTGGAC TATGTCATTC GTCATTTGAT CCAAGAAAAG 
AATAGAGTAA AGACAAGGGT ATTTCTGCAT GTTTTGAGTG AGCTTAAAGA AGTGGACCCA 
AAACTAGCCA CCTGGATCCA GAAACTGAAT AGCTTTGGCT TAGATGCCAC AGGACCTTTC 
TTTTCTCGTT ATGGGGCTCC TCTTAAGGAG CTTGATTTTA GCATCATGAC TTTTCTCTGG 
AATGAGAAAT ACGGTCATAA GCTAGGCTCT ATAGAAGGAA AGCAACTTGA TTATTTCTAT 
GAGCCTACAT CACTGAAGGA AGCGCGGTGT TTAATATGGC TGCTAGAAGA ACACAGAGAC 
AAGTTCCCAG CACTGCATAA TGCCTTGGAC GAGTTCTTTG ATATAATGGA CAGCCGCTGC 
ACTGTATTAA GGAAACAGGA CAGTGGTGAC GTGCCATTTA ATTCTACCAA GATAAAAAAC 
AAAGGCAAGA AAAAGAAGCC AAAAGATTCA AAGCCTATGT TAGTTGGATC TGGAACACCT 
TCAGTCACAC CAAACAATGA GACTGTCACT TCAGGTGAAG ACCATATCAA ACGCAGTTTA 
AGTTCTGCGG GCCCCTTTGT AGTGCCTGAT CACCTTCGGC AGGATATAGA AGAATTTGAA 
GCTCTGTATG AACAGCATAG TAATGAGTAT GTTGTCCGTA ATAAGAAGCT GTGGGACATA 
AACCCAAAAC AGAAATGTTC AACTCTATAC GATTATTTCT CTCAGTTGTT GGAGGAACAT 
GGTCCCTTGG ACATGAGTAA CAAAATGTTT TCTGAAGAAT ATGAGTTCTT CCCAGAAGAA 
ACTCAACAGA TACTAGAAAA AGCAGGAGGC TTGAAAGCGT TTCTCCTGGG ATGTCCTCGT 
TTTGTTGTGA TTGATAACTG TATCGCATTG AAGAAAGTTG CATTACGGCT CAAGAAAAAG 
AGAAAGAAGA AAAACATTAA AACAAAAGTA GAAGAAATTT CAAAAACAGG AGAGTATTTA 
CGAGTTAAAC TACCACTGAA TCCAACTGCT AGGGAATTTA AACCAGATGT AAAGTCCAAA 
CCAGTGTCTG ACTTACCTTC AGCACCAGCT TCTGAGGACA TGAAGCCTCA GCCTGCACCT 
GCTAATTCTC CCCAATCAGC TCGTGACACT GGGAAGCCAA AACCAGTTTT CGATAATTCT 
TCCAGATCAG TTTCTGAGGA TGAGAAGCCC AAAGGGATGT CTTCTGATTC TCCCAGGCCA 
GTCTTTGAGG ATACCGGTCA CAAGCCGGTC TCCTCTAATT CTCCCAAACC AGTTCCTGAG 
GAAGTGAAAG CGACCTATTG GCCTCGACCC CAGTTGGTCA CAGGATACTG TACATATCTT 
CCCTTTCGGG GATTTGACAT TACCCAGACT CCACCAGCCT TCATCAGTGT GTTACCGACT 
TTGCCCCAGT ATAGCATTTA CACCCCTCTG GCCAGTGTTC CTACTGAGTA TCACCTGCAA 
AGACCAATAC CAGTGGTGCC GTCTTTTGTA GCCAGTGACA TAACGGAAGT GCATTTTGAG 
GCTCATCATT TCAGTGCTGA GAATGCCCCT GGTACCCAGA TTGCCTCCGA AACACAGATC 
CTTGAGGAAT CTTTGGAAAC ACCAGTGAAG TCAGAGTGCA GCTATGATGG TGCTGATATA 
GTCCAGAGTG AGGCTAGCAG AAACGATGAC TACTGTGGCG ATTCTGCCAA ATGGGAAGTA 
AATCCAGAAA GCACCAATGA AGTAACAAAT ATTCCATGTA CACAAGTGGT TGCTGTACAG 
GTGTCTTGGA ATATAACACA TCAGGAAGTC AATACTGAGC CATATGGTCC TTTTGAGGCA 
CAACAGGGGG GTATTTCACA GGTTGAAAAG GAGTACCAGG TTTTACAAGA GCAGCTTAAG 
GAAGCATGTG AAAATTATGA ACACAGCAAT GTCAAGGGCT CAGAAGAAAC CAAGGACTTG 
GAAGAAAAGT TGAAAAGGAA TGTTGAAGAA AATAAGATCT CAAAGACAGA ACTAGATTGG 
TTCCTTGAAG ATTTGAACAA GGAAATTAAA AAATGGGAAC AGGAGAAAAA AGAAATCCAA 
GAAAGACTAA AGGCACTGAA GAAGAAAATG AAAAAGGTGT TAAATGCCAG CAAAATGTAT 
ACGCAGGAAA ATGATGGAAA GGAAAAGGCA CTTGAATTAC TTCTGGATCA GTCCCTTGAA 
ATCAGTAATA CATTTACAAA TGAGAAAATG AAAATAGAAG AGAGTATAAA GAAAGGGAAA 
GAGCATTATG AAGAGAGTCT TCAAAGGGCT GTGGATGCAG AGGTATCTGT GCTTGAAAAC 
TGGAAGGACA TGGAAGTGTG TAAGTTACAG AGCATGGAGT CACAAGCCGA AAGGTTTCTC 
AAGAATCTGA AGCTAATGAG CAGTGATTCG GCAGCGTATT CTGACATGGA CTCAGACATA 
CATTCATGGG AATCGTTTCT TTCTGATGTT AGAGAAGAAA TTGAGAAAGC AAAGTCTCAG 
TTTGAAGAAC AAATTCAGGC AATTAAATAT GGTTCTCAGC TCAGTGAACT TCCTAAAGTG 
CAGATTTCTG AGCTTTCATT TCCTACCTGT AACACGATTC ATCCTAAATT ACTTCCTGAG 
TCTTCAGGCC AGGATGGTCA AGGTCCCTCC ACTTCTACAA GTCATGGGAC CGGAGACCAG 
ACAGCAGCTC CCGAGGAGTC AAGTCTGGGC TCTGCAGCTG ACAGCCCAGT GGAGTTGCCC 
TCCTGCAGTC AGAAAGCCCC CCAGCTGCCA GAGCATGAGG GTGCTGGCCA GGCAACTGGG 
CCAGAACCAG GCCCAGTGGC TGATCAGAAG TTACCTGCTG CTCCAGAGCG TGCCACTCAT 
TCAAGCCAGT CTCCAAAGAA GCCGTTCAAT AGCATTATTG AGCACCTGTT GGTGGTGTTC 
CCGTGTTACA GCAGCACGGA GCTTGCTGGT TTTATTAAAA AAGTTAGAAA CAAAAACAAG 
ACCTCTCTGT CTGGATTGAG TATTGACGAA ATTGTGCAAA GAGTGACAGA ACACGTCCTT 
GATGAAGAAA GAAAGAAAAA GCCAAGTCCA GGAAAGGGCT GGAGGCTGTC TGCACCCAGC 
TCTGCTGTTG CTGTGCCCAG GGCTGCCCAG GACCCGCCCT CCATGCCTGT CGGAGCATCG 
CCCGAAACCA GGGGGCCGAG GACCGAGGAC GTCCCTGTGT CCTGCGCAAA TTCCTGTGAA 
ATGTGCCGAG AAGTATTCAA GTCCAAACAC GTGCGTGAGC TGCAGTGTGG GCACAGGCTT 
CACAAGGGGT GCTTCAAGCA GTGGCTGAAG AGCCAGCGCA CCTGCCCAGC CTGCCTGGGT 
CACGACCTCC TGTCAGAGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855693.1
CDS	90..5273
Translation	MPENHLLYGNRALCFLRTGQFRNALGDGKRATILKNNWTKGHYRYCAALSMLGEYDWALQANIKAQKLCKNDPEGIKDLIQQHVKLQKQIEDLQGQTPASNPVKAFCESRAYIPSLSAPAFSTSLNFVETEKNFRKTNHEMANGGNQNPKVVDEALKVDDCDCHPEFVPPSSQPPKYKGKQKSRNNELEKFSPGSQETLPVDLKNILEKQFSKSSRAAHQDFANIMKMLRSLIQDGYTALLEQRCRSAAQAFTELLNGLDPQKIKQLNLAMINYVLVVYGLAISLLGIGQPEELSEAENQFKRMIEHYPNEGLDCLAYCGIGKVYLKKNRFLEALNHFEKARTLICRLPGVLTWPTSNVVIEETQPGKIKMLLEKFVEECRFPPVPDATCCYQKCHGYSKIQIYITDPDFKGFIRISCCQYCKIEFHMNCWKKLKTTTFNDKIDKDFLQGICLTPDCEGIISKIIIFSSGGQVKCEFEHKVIKEKVPPRPILKQKCSSLEKLRLKEDKKLKRKLQKKEAKKLAQERLEEDLRESNPPKTEEQRETINHVQSCQFLDDRILQCIKQYADKIKSGILNTSKLLKELLSWKVLTTEDYTTCFSSRNFLNEAVDYVIRHLIQEKNRVKTRVFLHVLSELKEVDPKLATWIQKLNSFGLDATGPFFSRYGAPLKELDFSIMTFLWNEKYGHKLGSIEGKQLDYFYEPTSLKEARCLIWLLEEHRDKFPALHNALDEFFDIMDSRCTVLRKQDSGDVPFNSTKIKNKGKKKKPKDSKPMLVGSGTPSVTPNNETVTSGEDHIKRSLSSAGPFVVPDHLRQDIEEFEALYEQHSNEYVVRNKKLWDINPKQKCSTLYDYFSQLLEEHGPLDMSNKMFSEEYEFFPEETQQILEKAGGLKAFLLGCPRFVVIDNCIALKKVALRLKKKRKKKNIKTKVEEISKTGEYLRVKLPLNPTAREFKPDVKSKPVSDLPSAPASEDMKPQPAPANSPQSARDTGKPKPVFDNSSRSVSEDEKPKGMSSDSPRPVFEDTGHKPVSSNSPKPVPEEVKATYWPRPQLVTGYCTYLPFRGFDITQTPPAFISVLPTLPQYSIYTPLASVPTEYHLQRPIPVVPSFVASDITEVHFEAHHFSAENAPGTQIASETQILEESLETPVKSECSYDGADIVQSEASRNDDYCGDSAKWEVNPESTNEVTNIPCTQVVAVQVSWNITHQEVNTEPYGPFEAQQGGISQVEKEYQVLQEQLKEACENYEHSNVKGSEETKDLEEKLKRNVEENKISKTELDWFLEDLNKEIKKWEQEKKEIQERLKALKKKMKKVLNASKMYTQENDGKEKALELLLDQSLEISNTFTNEKMKIEESIKKGKEHYEESLQRAVDAEVSVLENWKDMEVCKLQSMESQAERFLKNLKLMSSDSAAYSDMDSDIHSWESFLSDVREEIEKAKSQFEEQIQAIKYGSQLSELPKVQISELSFPTCNTIHPKLLPESSGQDGQGPSTSTSHGTGDQTAAPEESSLGSAADSPVELPSCSQKAPQLPEHEGAGQATGPEPGPVADQKLPAAPERATHSSQSPKKPFNSIIEHLLVVFPCYSSTELAGFIKKVRNKNKTSLSGLSIDEIVQRVTEHVLDEERKKKPSPGKGWRLSAPSSAVAVPRAAQDPPSMPVGASPETRGPRTEDVPVSCANSCEMCREVFKSKHVRELQCGHRLHKGCFKQWLKSQRTCPACLGHDLLSEE

Target ORF information:

RefSeq Version	XM_024999925.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999925.1

ORF Insert Sequence:

ATGCCTGAAA ATCACCTGCT TTATGGTAAC CGAGCTCTTT GTTTTCTTCG TACTGGACAA 
TTCAGAAATG CACTTGGTGA TGGAAAGAGA GCTACCATTC TGAAGAACAA TTGGACAAAG 
GGTCATTATC GTTATTGTGC TGCTCTTTCT ATGCTGGGGG AATATGACTG GGCCCTGCAA 
GCAAACATAA AAGCTCAAAA ACTCTGTAAA AATGACCCTG AGGGAATCAA GGATCTAATT 
CAGCAGCATG TAAAGTTACA AAAACAAATA GAAGACCTGC AAGGTCAAAC ACCAGCTAGT 
AATCCAGTCA AAGCCTTCTG TGAAAGCAGG GCCTACATAC CTAGTTTATC AGCACCTGCA 
TTTAGTACTT CACTTAACTT TGTGGAAACT GAAAAAAATT TCAGAAAAAC TAACCACGAA 
ATGGCAAACG GTGGTAATCA GAATCCAAAG GTGGTAGATG AAGCTCTGAA AGTAGATGAT 
TGTGACTGTC ATCCTGAATT TGTACCGCCA TCAAGTCAGC CTCCAAAATA TAAAGGGAAA 
CAAAAATCTC GAAACAATGA ATTGGAGAAG TTCAGCCCTG GTTCACAAGA AACTTTACCA 
GTAGATTTAA AAAACATCTT GGAGAAACAG TTTTCTAAAT CTTCCAGAGC CGCACACCAG 
GATTTTGCTA ATATAATGAA AATGTTGAGA AGTTTAATTC AGGATGGCTA CACGGCCTTG 
CTGGAGCAGC GCTGCCGCAG CGCCGCCCAA GCCTTCACAG AGTTGCTCAA CGGTTTAGAT 
CCTCAAAAAA TAAAGCAATT AAACCTGGCC ATGATTAACT ACGTGTTAGT AGTCTACGGA 
CTTGCCATTT CACTCCTTGG AATAGGACAG CCCGAGGAGT TATCTGAAGC TGAAAACCAG 
TTCAAGAGGA TGATTGAACA CTACCCCAAT GAGGGGCTTG ATTGCTTGGC CTACTGTGGA 
ATTGGAAAAG TGTATTTGAA AAAAAACAGA TTTTTAGAAG CTCTTAATCA TTTTGAGAAA 
GCAAGAACCT TGATCTGTCG TCTTCCTGGA GTGTTAACTT GGCCCACAAG TAATGTGGTT 
ATTGAGGAGA CTCAGCCAGG GAAGATAAAG ATGCTGTTAG AGAAATTTGT TGAAGAATGC 
AGGTTCCCTC CAGTGCCGGA TGCCACTTGT TGCTATCAGA AGTGCCATGG ATATTCCAAA 
ATCCAGATAT ATATAACTGA TCCAGACTTT AAGGGTTTTA TACGGATCAG CTGTTGCCAG 
TATTGTAAAA TAGAATTTCA CATGAACTGC TGGAAGAAGT TAAAAACAAC AACCTTCAAT 
GATAAAATTG ACAAGGATTT TCTGCAAGGA ATCTGTCTTA CTCCTGATTG TGAAGGAATC 
ATTTCAAAGA TTATCATCTT CAGCAGTGGT GGTCAAGTTA AATGTGAATT TGAACACAAG 
GTCATTAAAG AAAAGGTTCC TCCAAGACCT ATTCTGAAAC AGAAATGTTC TAGCCTAGAG 
AAGTTGAGGC TGAAAGAAGA CAAAAAATTG AAGCGAAAGC TCCAAAAAAA GGAAGCAAAA 
AAATTAGCAC AAGAAAGACT GGAAGAGGAC TTAAGGGAAA GTAACCCACC AAAAACTGAA 
GAGCAGAGAG AAACTATCAA CCATGTCCAA AGTTGCCAGT TCCTTGATGA CAGAATTCTG 
CAGTGCATAA AGCAGTACGC TGACAAGATT AAGTCCGGGA TACTGAATAC TTCCAAGCTT 
CTCAAAGAAT TGCTTTCTTG GAAGGTTTTA ACCACAGAAG ACTACACAAC GTGTTTTTCT 
AGCAGAAATT TCCTGAATGA AGCAGTGGAC TATGTCATTC GTCATTTGAT CCAAGAAAAG 
AATAGAGTAA AGACAAGGGT ATTTCTGCAT GTTTTGAGTG AGCTTAAAGA AGTGGACCCA 
AAACTAGCCA CCTGGATCCA GAAACTGAAT AGCTTTGGCT TAGATGCCAC AGGACCTTTC 
TTTTCTCGTT ATGGGGCTCC TCTTAAGGAG CTTGATTTTA GCATCATGAC TTTTCTCTGG 
AATGAGAAAT ACGGTCATAA GCTAGGCTCT ATAGAAGGAA AGCAACTTGA TTATTTCTAT 
GAGCCTACAT CACTGAAGGA AGCGCGGTGT TTAATATGGC TGCTAGAAGA ACACAGAGAC 
AAGTTCCCAG CACTGCATAA TGCCTTGGAC GAGTTCTTTG ATATAATGGA CAGCCGCTGC 
ACTGTATTAA GGAAACAGGA CAGTGGTGAC GTGCCATTTA ATTCTACCAA GATAAAAAAC 
AAAGGCAAGA AAAAGAAGCC AAAAGATTCA AAGCCTATGT TAGTTGGATC TGGAACACCT 
TCAGTCACAC CAAACAATGA GACTGTCACT TCAGGTGAAG ACCATATCAA ACGCAGTTTA 
AGTTCTGCGG GCCCCTTTGT AGTGCCTGAT CACCTTCGGC AGGATATAGA AGAATTTGAA 
GCTCTGTATG AACAGCATAG TAATGAGTAT GTTGTCCGTA ATAAGAAGCT GTGGGACATA 
AACCCAAAAC AGAAATGTTC AACTCTATAC GATTATTTCT CTCAGTTGTT GGAGGAACAT 
GGTCCCTTGG ACATGAGTAA CAAAATGTTT TCTGAAGAAT ATGAGTTCTT CCCAGAAGAA 
ACTCAACAGA TACTAGAAAA AGCAGGAGGC TTGAAAGCGT TTCTCCTGGG ATGTCCTCGT 
TTTGTTGTGA TTGATAACTG TATCGCATTG AAGAAAGTTG CATTACGGCT CAAGAAAAAG 
AGAAAGAAGA AAAACATTAA AACAAAAGTA GAAGAAATTT CAAAAACAGG AGAGTATTTA 
CGAGTTAAAC TACCACTGAA TCCAACTGCT AGGGAATTTA AACCAGATGT AAAGTCCAAA 
CCAGTGTCTG ACTTACCTTC AGCACCAGCT TCTGAGGACA TGAAGCCTCA GCCTGCACCT 
GCTAATTCTC CCCAATCAGC TCGTGACACT GGGAAGCCAA AACCAGTTTT CGATAATTCT 
TCCAGATCAG TTTCTGAGGA TGAGAAGCCC AAAGGGATGT CTTCTGATTC TCCCAGGCCA 
GTCTTTGAGG ATACCGGTCA CAAGCCGGTC TCCTCTAATT CTCCCAAACC AGTTCCTGAG 
GAAGTGAAAG CGACCTATTG GCCTCGACCC CAGTTGGTCA CAGGATACTG TACATATCTT 
CCCTTTCGGG GATTTGACAT TACCCAGACT CCACCAGCCT TCATCAGTGT GTTACCGACT 
TTGCCCCAGT ATAGCATTTA CACCCCTCTG GCCAGTGTTC CTACTGAGTA TCACCTGCAA 
AGACCAATAC CAGTGGTGCC GTCTTTTGTA GCCAGTGACA TAACGGAAGT GCATTTTGAG 
GCTCATCATT TCAGTGCTGA GAATGCCCCT GGTACCCAGA TTGCCTCCGA AACACAGATC 
CTTGAGGAAT CTTTGGAAAC ACCAGTGAAG TCAGAGTGCA GCTATGATGG TGCTGATATA 
GTCCAGAGTG AGGCTAGCAG AAACGATGAC TACTGTGGCG ATTCTGCCAA ATGGGAAGTA 
AATCCAGAAA GCACCAATGA AGTAACAAAT ATTCCATGTA CACAAGTGGT TGCTGTACAG 
GTGTCTTGGA ATATAACACA TCAGGAAGTC AATACTGAGC CATATGGTCC TTTTGAGGCA 
CAACAGGGGG GTATTTCACA GGTTGAAAAG GAGTACCAGG TTTTACAAGA GCAGCTTAAG 
GAAGCATGTG AAAATTATGA ACACAGCAAT GTCAAGGGCT CAGAAGAAAC CAAGGACTTG 
GAAGAAAAGT TGAAAAGGAA TGTTGAAGAA AATAAGATCT CAAAGACAGA ACTAGATTGG 
TTCCTTGAAG ATTTGAACAA GGAAATTAAA AAATGGGAAC AGGAGAAAAA AGAAATCCAA 
GAAAGACTAA AGGCACTGAA GAAGAAAATG AAAAAGGTGT TAAATGCCAG CAAAATGTAT 
ACGCAGGAAA ATGATGGAAA GGAAAAGGCA CTTGAATTAC TTCTGGATCA GTCCCTTGAA 
ATCAGTAATA CATTTACAAA TGAGAAAATG AAAATAGAAG AGAGTATAAA GAAAGGGAAA 
GAGCATTATG AAGAGAGTCT TCAAAGGGCT GTGGATGCAG AGGTATCTGT GCTTGAAAAC 
TGGAAGGACA TGGAAGTGTG TAAGTTACAG AGCATGGAGT CACAAGCCGA AAGGTTTCTC 
AAGAATCTGA AGCTAATGAG CAGTGATTCG GCAGCGTATT CTGACATGGA CTCAGACATA 
CATTCATGGG AATCGTTTCT TTCTGATGTT AGAGAAGAAA TTGAGAAAGC AAAGTCTCAG 
TTTGAAGAAC AAATTCAGGC AATTAAATAT GGTTCTCAGC TCAGTGAACT TCCTAAAGTG 
CAGATTTCTG AGCTTTCATT TCCTACCTGT AACACGATTC ATCCTAAATT ACTTCCTGAG 
TCTTCAGGCC AGGATGGTCA AGGTCCCTCC ACTTCTACAA GTCATGGGAC CGGAGACCAG 
ACAGCAGCTC CCGAGGAGTC AAGTCTGGGC TCTGCAGCTG ACAGCCCAGT GGAGTTGCCC 
TCCTGCAGTC AGAAAGCCCC CCAGCTGCCA GAGCATGAGG GTGCTGGCCA GGCAACTGGG 
CCAGAACCAG GCCCAGTGGC TGATCAGAAG TTACCTGCTG CTCCAGAGCG TGCCACTCAT 
TCAAGCCAGT CTCCAAAGAA GCCGTTCAAT AGCATTATTG AGCACCTGTT GGTGGTGTTC 
CCGTGTTACA GCAGCACGGA GCTTGCTGGT TTTATTAAAA AAGTTAGAAA CAAAAACAAG 
ACCTCTCTGT CTGGATTGAG TATTGACGAA ATTGTGCAAA GAGTGACAGA ACACGTCCTT 
GATGAAGAAA GAAAGAAAAA GCCAAGTCCA GGAAAGGGCT GGAGGCTGTC TGCACCCAGC 
TCTGCTGTTG CTGTGCCCAG GGCTGCCCAG GACCCGCCCT CCATGCCTGT CGGAGCATCG 
CCCGAAACCA GGGGGCCGAG GACCGAGGAC GTCCCTGTGT CCTGCGCAAA TTCCTGTGAA 
ATGTGCCGAG AAGTATTCAA GTCCAAACAC GTGCGTGAGC TGCAGTGTGG GCACAGGCTT 
CACAAGGGGT GCTTCAAGCA GTGGCTGAAG AGCCAGCGCA CCTGCCCAGC CTGCCTGGGT 
CACGACCTCC TGTCAGAGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_005202060.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005202117.1 Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X12, mRNA.

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CloneID	OBa218661
Clone ID Related Accession (Same CDS sequence)	XM_005202060.2 , XM_005202061.4 , XM_024999992.1
Accession Version	XM_005202060.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5034bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TTC3 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005202060.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGGGG AATATGACTG GGCCCTGCAA GCAAACATAA AAGCTCAAAA ACTCTGTAAA 
AATGACCCTG AGGGAATCAA GGATCTAATT CAGCAGCATG TAAAGTTACA AAAACAAATA 
GAAGACCTGC AAGGTCAAAC ACCAGCTAGT AATCCAGTCA AAGCCTTCTG TGAAAGCAGG 
GCCTACATAC CTAGTTTATC AGCACCTGCA TTTAGTACTT CACTTAACTT TGTGGAAACT 
GAAAAAAATT TCAGAAAAAC TAACCACGAA ATGGCAAACG GTGGTAATCA GAATCCAAAG 
GTGGTAGATG AAGCTCTGAA AGTAGATGAT TGTGACTGTC ATCCTGAATT TGTACCGCCA 
TCAAGTCAGC CTCCAAAATA TAAAGGGAAA CAAAAATCTC GAAACAATGA ATTGGAGAAG 
TTCAGCCCTG GTTCACAAGA AACTTTACCA GTAGATTTAA AAAACATCTT GGAGAAACAG 
TTTTCTAAAT CTTCCAGAGC CGCACACCAG GATTTTGCTA ATATAATGAA AATGTTGAGA 
AGTTTAATTC AGGATGGCTA CACGGCCTTG CTGGAGCAGC GCTGCCGCAG CGCCGCCCAA 
GCCTTCACAG AGTTGCTCAA CGGTTTAGAT CCTCAAAAAA TAAAGCAATT AAACCTGGCC 
ATGATTAACT ACGTGTTAGT AGTCTACGGA CTTGCCATTT CACTCCTTGG AATAGGACAG 
CCCGAGGAGT TATCTGAAGC TGAAAACCAG TTCAAGAGGA TGATTGAACA CTACCCCAAT 
GAGGGGCTTG ATTGCTTGGC CTACTGTGGA ATTGGAAAAG TGTATTTGAA AAAAAACAGA 
TTTTTAGAAG CTCTTAATCA TTTTGAGAAA GCAAGAACCT TGATCTGTCG TCTTCCTGGA 
GTGTTAACTT GGCCCACAAG TAATGTGGTT ATTGAGGAGA CTCAGCCAGG GAAGATAAAG 
ATGCTGTTAG AGAAATTTGT TGAAGAATGC AGGTTCCCTC CAGTGCCGGA TGCCACTTGT 
TGCTATCAGA AGTGCCATGG ATATTCCAAA ATCCAGATAT ATATAACTGA TCCAGACTTT 
AAGGGTTTTA TACGGATCAG CTGTTGCCAG TATTGTAAAA TAGAATTTCA CATGAACTGC 
TGGAAGAAGT TAAAAACAAC AACCTTCAAT GATAAAATTG ACAAGGATTT TCTGCAAGGA 
ATCTGTCTTA CTCCTGATTG TGAAGGAATC ATTTCAAAGA TTATCATCTT CAGCAGTGGT 
GGTCAAGTTA AATGTGAATT TGAACACAAG GTCATTAAAG AAAAGGTTCC TCCAAGACCT 
ATTCTGAAAC AGAAATGTTC TAGCCTAGAG AAGTTGAGGC TGAAAGAAGA CAAAAAATTG 
AAGCGAAAGC TCCAAAAAAA GGAAGCAAAA AAATTAGCAC AAGAAAGACT GGAAGAGGAC 
TTAAGGGAAA GTAACCCACC AAAAACTGAA GAGCAGAGAG AAACTATCAA CCATGTCCAA 
AGTTGCCAGT TCCTTGATGA CAGAATTCTG CAGTGCATAA AGCAGTACGC TGACAAGATT 
AAGTCCGGGA TACTGAATAC TTCCAAGCTT CTCAAAGAAT TGCTTTCTTG GAAGGTTTTA 
ACCACAGAAG ACTACACAAC GTGTTTTTCT AGCAGAAATT TCCTGAATGA AGCAGTGGAC 
TATGTCATTC GTCATTTGAT CCAAGAAAAG AATAGAGTAA AGACAAGGGT ATTTCTGCAT 
GTTTTGAGTG AGCTTAAAGA AGTGGACCCA AAACTAGCCA CCTGGATCCA GAAACTGAAT 
AGCTTTGGCT TAGATGCCAC AGGACCTTTC TTTTCTCGTT ATGGGGCTCC TCTTAAGGAG 
CTTGATTTTA GCATCATGAC TTTTCTCTGG AATGAGAAAT ACGGTCATAA GCTAGGCTCT 
ATAGAAGGAA AGCAACTTGA TTATTTCTAT GAGCCTACAT CACTGAAGGA AGCGCGGTGT 
TTAATATGGC TGCTAGAAGA ACACAGAGAC AAGTTCCCAG CACTGCATAA TGCCTTGGAC 
GAGTTCTTTG ATATAATGGA CAGCCGCTGC ACTGTATTAA GGAAACAGGA CAGTGGTGAC 
GTGCCATTTA ATTCTACCAA GATAAAAAAC AAAGGCAAGA AAAAGAAGCC AAAAGATTCA 
AAGCCTATGT TAGTTGGATC TGGAACACCT TCAGTCACAC CAAACAATGA GACTGTCACT 
TCAGGTGAAG ACCATATCAA ACGCAGTTTA AGTTCTGCGG GCCCCTTTGT AGTGCCTGAT 
CACCTTCGGC AGGATATAGA AGAATTTGAA GCTCTGTATG AACAGCATAG TAATGAGTAT 
GTTGTCCGTA ATAAGAAGCT GTGGGACATA AACCCAAAAC AGAAATGTTC AACTCTATAC 
GATTATTTCT CTCAGTTGTT GGAGGAACAT GGTCCCTTGG ACATGAGTAA CAAAATGTTT 
TCTGAAGAAT ATGAGTTCTT CCCAGAAGAA ACTCAACAGA TACTAGAAAA AGCAGGAGGC 
TTGAAAGCGT TTCTCCTGGG ATGTCCTCGT TTTGTTGTGA TTGATAACTG TATCGCATTG 
AAGAAAGTTG CATTACGGCT CAAGAAAAAG AGAAAGAAGA AAAACATTAA AACAAAAGTA 
GAAGAAATTT CAAAAACAGG AGAGTATTTA CGAGTTAAAC TACCACTGAA TCCAACTGCT 
AGGGAATTTA AACCAGATGT AAAGTCCAAA CCAGTGTCTG ACTTACCTTC AGCACCAGCT 
TCTGAGGACA TGAAGCCTCA GCCTGCACCT GCTAATTCTC CCCAATCAGC TCGTGACACT 
GGGAAGCCAA AACCAGTTTT CGATAATTCT TCCAGATCAG TTTCTGAGGA TGAGAAGCCC 
AAAGGGATGT CTTCTGATTC TCCCAGGCCA GTCTTTGAGG ATACCGGTCA CAAGCCGGTC 
TCCTCTAATT CTCCCAAACC AGTTCCTGAG GAAGTGAAAG CGACCTATTG GCCTCGACCC 
CAGTTGGTCA CAGGATACTG TACATATCTT CCCTTTCGGG GATTTGACAT TACCCAGACT 
CCACCAGCCT TCATCAGTGT GTTACCGACT TTGCCCCAGT ATAGCATTTA CACCCCTCTG 
GCCAGTGTTC CTACTGAGTA TCACCTGCAA AGACCAATAC CAGTGGTGCC GTCTTTTGTA 
GCCAGTGACA TAACGGAAGT GCATTTTGAG GCTCATCATT TCAGTGCTGA GAATGCCCCT 
GGTACCCAGA TTGCCTCCGA AACACAGATC CTTGAGGAAT CTTTGGAAAC ACCAGTGAAG 
TCAGAGTGCA GCTATGATGG TGCTGATATA GTCCAGAGTG AGGCTAGCAG AAACGATGAC 
TACTGTGGCG ATTCTGCCAA ATGGGAAGTA AATCCAGAAA GCACCAATGA AGTAACAAAT 
ATTCCATGTA CACAAGTGGT TGCTGTACAG GTGTCTTGGA ATATAACACA TCAGGAAGTC 
AATACTGAGC CATATGGTCC TTTTGAGGCA CAACAGGGGG GTATTTCACA GGTTGAAAAG 
GAGTACCAGG TTTTACAAGA GCAGCTTAAG GAAGCATGTG AAAATTATGA ACACAGCAAT 
GTCAAGGGCT CAGAAGAAAC CAAGGACTTG GAAGAAAAGT TGAAAAGGAA TGTTGAAGAA 
AATAAGATCT CAAAGACAGA ACTAGATTGG TTCCTTGAAG ATTTGAACAA GGAAATTAAA 
AAATGGGAAC AGGAGAAAAA AGAAATCCAA GAAAGACTAA AGGCACTGAA GAAGAAAATG 
AAAAAGGTGT TAAATGCCAG CAAAATGTAT ACGCAGGAAA ATGATGGAAA GGAAAAGGCA 
CTTGAATTAC TTCTGGATCA GTCCCTTGAA ATCAGTAATA CATTTACAAA TGAGAAAATG 
AAAATAGAAG AGAGTATAAA GAAAGGGAAA GAGCATTATG AAGAGAGTCT TCAAAGGGCT 
GTGGATGCAG AGGTATCTGT GCTTGAAAAC TGGAAGGACA TGGAAGTGTG TAAGTTACAG 
AGCATGGAGT CACAAGCCGA AAGGTTTCTC AAGAATCTGA AGCTAATGAG CAGTGATTCG 
GCAGCGTATT CTGACATGGA CTCAGACATA CATTCATGGG AATCGTTTCT TTCTGATGTT 
AGAGAAGAAA TTGAGAAAGC AAAGTCTCAG TTTGAAGAAC AAATTCAGGC AATTAAATAT 
GGTTCTCAGC TCAGTGAACT TCCTAAAGTG CAGATTTCTG AGCTTTCATT TCCTACCTGT 
AACACGATTC ATCCTAAATT ACTTCCTGAG TCTTCAGGCC AGGATGGTCA AGGTCCCTCC 
ACTTCTACAA GTCATGGGAC CGGAGACCAG ACAGCAGCTC CCGAGGAGTC AAGTCTGGGC 
TCTGCAGCTG ACAGCCCAGT GGAGTTGCCC TCCTGCAGTC AGAAAGCCCC CCAGCTGCCA 
GAGCATGAGG GTGCTGGCCA GGCAACTGGG CCAGAACCAG GCCCAGTGGC TGATCAGAAG 
TTACCTGCTG CTCCAGAGCG TGCCACTCAT TCAAGCCAGT CTCCAAAGAA GCCGTTCAAT 
AGCATTATTG AGCACCTGTT GGTGGTGTTC CCGTGTTACA GCAGCACGGA GCTTGCTGGT 
TTTATTAAAA AAGTTAGAAA CAAAAACAAG ACCTCTCTGT CTGGATTGAG TATTGACGAA 
ATTGTGCAAA GAGTGACAGA ACACGTCCTT GATGAAGAAA GAAAGAAAAA GCCAAGTCCA 
GGAAAGGGCT GGAGGCTGTC TGCACCCAGC TCTGCTGTTG CTGTGCCCAG GGCTGCCCAG 
GACCCGCCCT CCATGCCTGT CGGAGCATCG CCCGAAACCA GGGGGCCGAG GACCGAGGAC 
GTCCCTGTGT CCTGCGCAAA TTCCTGTGAA ATGTGCCGAG AAGTATTCAA GTCCAAACAC 
GTGCGTGAGC TGCAGTGTGG GCACAGGCTT CACAAGGGGT GCTTCAAGCA GTGGCTGAAG 
AGCCAGCGCA CCTGCCCAGC CTGCCTGGGT CACGACCTCC TGTCAGAGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005202117.1
CDS	265..5298
Translation	MLGEYDWALQANIKAQKLCKNDPEGIKDLIQQHVKLQKQIEDLQGQTPASNPVKAFCESRAYIPSLSAPAFSTSLNFVETEKNFRKTNHEMANGGNQNPKVVDEALKVDDCDCHPEFVPPSSQPPKYKGKQKSRNNELEKFSPGSQETLPVDLKNILEKQFSKSSRAAHQDFANIMKMLRSLIQDGYTALLEQRCRSAAQAFTELLNGLDPQKIKQLNLAMINYVLVVYGLAISLLGIGQPEELSEAENQFKRMIEHYPNEGLDCLAYCGIGKVYLKKNRFLEALNHFEKARTLICRLPGVLTWPTSNVVIEETQPGKIKMLLEKFVEECRFPPVPDATCCYQKCHGYSKIQIYITDPDFKGFIRISCCQYCKIEFHMNCWKKLKTTTFNDKIDKDFLQGICLTPDCEGIISKIIIFSSGGQVKCEFEHKVIKEKVPPRPILKQKCSSLEKLRLKEDKKLKRKLQKKEAKKLAQERLEEDLRESNPPKTEEQRETINHVQSCQFLDDRILQCIKQYADKIKSGILNTSKLLKELLSWKVLTTEDYTTCFSSRNFLNEAVDYVIRHLIQEKNRVKTRVFLHVLSELKEVDPKLATWIQKLNSFGLDATGPFFSRYGAPLKELDFSIMTFLWNEKYGHKLGSIEGKQLDYFYEPTSLKEARCLIWLLEEHRDKFPALHNALDEFFDIMDSRCTVLRKQDSGDVPFNSTKIKNKGKKKKPKDSKPMLVGSGTPSVTPNNETVTSGEDHIKRSLSSAGPFVVPDHLRQDIEEFEALYEQHSNEYVVRNKKLWDINPKQKCSTLYDYFSQLLEEHGPLDMSNKMFSEEYEFFPEETQQILEKAGGLKAFLLGCPRFVVIDNCIALKKVALRLKKKRKKKNIKTKVEEISKTGEYLRVKLPLNPTAREFKPDVKSKPVSDLPSAPASEDMKPQPAPANSPQSARDTGKPKPVFDNSSRSVSEDEKPKGMSSDSPRPVFEDTGHKPVSSNSPKPVPEEVKATYWPRPQLVTGYCTYLPFRGFDITQTPPAFISVLPTLPQYSIYTPLASVPTEYHLQRPIPVVPSFVASDITEVHFEAHHFSAENAPGTQIASETQILEESLETPVKSECSYDGADIVQSEASRNDDYCGDSAKWEVNPESTNEVTNIPCTQVVAVQVSWNITHQEVNTEPYGPFEAQQGGISQVEKEYQVLQEQLKEACENYEHSNVKGSEETKDLEEKLKRNVEENKISKTELDWFLEDLNKEIKKWEQEKKEIQERLKALKKKMKKVLNASKMYTQENDGKEKALELLLDQSLEISNTFTNEKMKIEESIKKGKEHYEESLQRAVDAEVSVLENWKDMEVCKLQSMESQAERFLKNLKLMSSDSAAYSDMDSDIHSWESFLSDVREEIEKAKSQFEEQIQAIKYGSQLSELPKVQISELSFPTCNTIHPKLLPESSGQDGQGPSTSTSHGTGDQTAAPEESSLGSAADSPVELPSCSQKAPQLPEHEGAGQATGPEPGPVADQKLPAAPERATHSSQSPKKPFNSIIEHLLVVFPCYSSTELAGFIKKVRNKNKTSLSGLSIDEIVQRVTEHVLDEERKKKPSPGKGWRLSAPSSAVAVPRAAQDPPSMPVGASPETRGPRTEDVPVSCANSCEMCREVFKSKHVRELQCGHRLHKGCFKQWLKSQRTCPACLGHDLLSEE

Target ORF information:

RefSeq Version	XM_005202060.2
Organism	Bos taurus(cattle)
Definition	Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005202060.2

ORF Insert Sequence:

ATGCTGGGGG AATATGACTG GGCCCTGCAA GCAAACATAA AAGCTCAAAA ACTCTGTAAA 
AATGACCCTG AGGGAATCAA GGATCTAATT CAGCAGCATG TAAAGTTACA AAAACAAATA 
GAAGACCTGC AAGGTCAAAC ACCAGCTAGT AATCCAGTCA AAGCCTTCTG TGAAAGCAGG 
GCCTACATAC CTAGTTTATC AGCACCTGCA TTTAGTACTT CACTTAACTT TGTGGAAACT 
GAAAAAAATT TCAGAAAAAC TAACCACGAA ATGGCAAACG GTGGTAATCA GAATCCAAAG 
GTGGTAGATG AAGCTCTGAA AGTAGATGAT TGTGACTGTC ATCCTGAATT TGTACCGCCA 
TCAAGTCAGC CTCCAAAATA TAAAGGGAAA CAAAAATCTC GAAACAATGA ATTGGAGAAG 
TTCAGCCCTG GTTCACAAGA AACTTTACCA GTAGATTTAA AAAACATCTT GGAGAAACAG 
TTTTCTAAAT CTTCCAGAGC CGCACACCAG GATTTTGCTA ATATAATGAA AATGTTGAGA 
AGTTTAATTC AGGATGGCTA CACGGCCTTG CTGGAGCAGC GCTGCCGCAG CGCCGCCCAA 
GCCTTCACAG AGTTGCTCAA CGGTTTAGAT CCTCAAAAAA TAAAGCAATT AAACCTGGCC 
ATGATTAACT ACGTGTTAGT AGTCTACGGA CTTGCCATTT CACTCCTTGG AATAGGACAG 
CCCGAGGAGT TATCTGAAGC TGAAAACCAG TTCAAGAGGA TGATTGAACA CTACCCCAAT 
GAGGGGCTTG ATTGCTTGGC CTACTGTGGA ATTGGAAAAG TGTATTTGAA AAAAAACAGA 
TTTTTAGAAG CTCTTAATCA TTTTGAGAAA GCAAGAACCT TGATCTGTCG TCTTCCTGGA 
GTGTTAACTT GGCCCACAAG TAATGTGGTT ATTGAGGAGA CTCAGCCAGG GAAGATAAAG 
ATGCTGTTAG AGAAATTTGT TGAAGAATGC AGGTTCCCTC CAGTGCCGGA TGCCACTTGT 
TGCTATCAGA AGTGCCATGG ATATTCCAAA ATCCAGATAT ATATAACTGA TCCAGACTTT 
AAGGGTTTTA TACGGATCAG CTGTTGCCAG TATTGTAAAA TAGAATTTCA CATGAACTGC 
TGGAAGAAGT TAAAAACAAC AACCTTCAAT GATAAAATTG ACAAGGATTT TCTGCAAGGA 
ATCTGTCTTA CTCCTGATTG TGAAGGAATC ATTTCAAAGA TTATCATCTT CAGCAGTGGT 
GGTCAAGTTA AATGTGAATT TGAACACAAG GTCATTAAAG AAAAGGTTCC TCCAAGACCT 
ATTCTGAAAC AGAAATGTTC TAGCCTAGAG AAGTTGAGGC TGAAAGAAGA CAAAAAATTG 
AAGCGAAAGC TCCAAAAAAA GGAAGCAAAA AAATTAGCAC AAGAAAGACT GGAAGAGGAC 
TTAAGGGAAA GTAACCCACC AAAAACTGAA GAGCAGAGAG AAACTATCAA CCATGTCCAA 
AGTTGCCAGT TCCTTGATGA CAGAATTCTG CAGTGCATAA AGCAGTACGC TGACAAGATT 
AAGTCCGGGA TACTGAATAC TTCCAAGCTT CTCAAAGAAT TGCTTTCTTG GAAGGTTTTA 
ACCACAGAAG ACTACACAAC GTGTTTTTCT AGCAGAAATT TCCTGAATGA AGCAGTGGAC 
TATGTCATTC GTCATTTGAT CCAAGAAAAG AATAGAGTAA AGACAAGGGT ATTTCTGCAT 
GTTTTGAGTG AGCTTAAAGA AGTGGACCCA AAACTAGCCA CCTGGATCCA GAAACTGAAT 
AGCTTTGGCT TAGATGCCAC AGGACCTTTC TTTTCTCGTT ATGGGGCTCC TCTTAAGGAG 
CTTGATTTTA GCATCATGAC TTTTCTCTGG AATGAGAAAT ACGGTCATAA GCTAGGCTCT 
ATAGAAGGAA AGCAACTTGA TTATTTCTAT GAGCCTACAT CACTGAAGGA AGCGCGGTGT 
TTAATATGGC TGCTAGAAGA ACACAGAGAC AAGTTCCCAG CACTGCATAA TGCCTTGGAC 
GAGTTCTTTG ATATAATGGA CAGCCGCTGC ACTGTATTAA GGAAACAGGA CAGTGGTGAC 
GTGCCATTTA ATTCTACCAA GATAAAAAAC AAAGGCAAGA AAAAGAAGCC AAAAGATTCA 
AAGCCTATGT TAGTTGGATC TGGAACACCT TCAGTCACAC CAAACAATGA GACTGTCACT 
TCAGGTGAAG ACCATATCAA ACGCAGTTTA AGTTCTGCGG GCCCCTTTGT AGTGCCTGAT 
CACCTTCGGC AGGATATAGA AGAATTTGAA GCTCTGTATG AACAGCATAG TAATGAGTAT 
GTTGTCCGTA ATAAGAAGCT GTGGGACATA AACCCAAAAC AGAAATGTTC AACTCTATAC 
GATTATTTCT CTCAGTTGTT GGAGGAACAT GGTCCCTTGG ACATGAGTAA CAAAATGTTT 
TCTGAAGAAT ATGAGTTCTT CCCAGAAGAA ACTCAACAGA TACTAGAAAA AGCAGGAGGC 
TTGAAAGCGT TTCTCCTGGG ATGTCCTCGT TTTGTTGTGA TTGATAACTG TATCGCATTG 
AAGAAAGTTG CATTACGGCT CAAGAAAAAG AGAAAGAAGA AAAACATTAA AACAAAAGTA 
GAAGAAATTT CAAAAACAGG AGAGTATTTA CGAGTTAAAC TACCACTGAA TCCAACTGCT 
AGGGAATTTA AACCAGATGT AAAGTCCAAA CCAGTGTCTG ACTTACCTTC AGCACCAGCT 
TCTGAGGACA TGAAGCCTCA GCCTGCACCT GCTAATTCTC CCCAATCAGC TCGTGACACT 
GGGAAGCCAA AACCAGTTTT CGATAATTCT TCCAGATCAG TTTCTGAGGA TGAGAAGCCC 
AAAGGGATGT CTTCTGATTC TCCCAGGCCA GTCTTTGAGG ATACCGGTCA CAAGCCGGTC 
TCCTCTAATT CTCCCAAACC AGTTCCTGAG GAAGTGAAAG CGACCTATTG GCCTCGACCC 
CAGTTGGTCA CAGGATACTG TACATATCTT CCCTTTCGGG GATTTGACAT TACCCAGACT 
CCACCAGCCT TCATCAGTGT GTTACCGACT TTGCCCCAGT ATAGCATTTA CACCCCTCTG 
GCCAGTGTTC CTACTGAGTA TCACCTGCAA AGACCAATAC CAGTGGTGCC GTCTTTTGTA 
GCCAGTGACA TAACGGAAGT GCATTTTGAG GCTCATCATT TCAGTGCTGA GAATGCCCCT 
GGTACCCAGA TTGCCTCCGA AACACAGATC CTTGAGGAAT CTTTGGAAAC ACCAGTGAAG 
TCAGAGTGCA GCTATGATGG TGCTGATATA GTCCAGAGTG AGGCTAGCAG AAACGATGAC 
TACTGTGGCG ATTCTGCCAA ATGGGAAGTA AATCCAGAAA GCACCAATGA AGTAACAAAT 
ATTCCATGTA CACAAGTGGT TGCTGTACAG GTGTCTTGGA ATATAACACA TCAGGAAGTC 
AATACTGAGC CATATGGTCC TTTTGAGGCA CAACAGGGGG GTATTTCACA GGTTGAAAAG 
GAGTACCAGG TTTTACAAGA GCAGCTTAAG GAAGCATGTG AAAATTATGA ACACAGCAAT 
GTCAAGGGCT CAGAAGAAAC CAAGGACTTG GAAGAAAAGT TGAAAAGGAA TGTTGAAGAA 
AATAAGATCT CAAAGACAGA ACTAGATTGG TTCCTTGAAG ATTTGAACAA GGAAATTAAA 
AAATGGGAAC AGGAGAAAAA AGAAATCCAA GAAAGACTAA AGGCACTGAA GAAGAAAATG 
AAAAAGGTGT TAAATGCCAG CAAAATGTAT ACGCAGGAAA ATGATGGAAA GGAAAAGGCA 
CTTGAATTAC TTCTGGATCA GTCCCTTGAA ATCAGTAATA CATTTACAAA TGAGAAAATG 
AAAATAGAAG AGAGTATAAA GAAAGGGAAA GAGCATTATG AAGAGAGTCT TCAAAGGGCT 
GTGGATGCAG AGGTATCTGT GCTTGAAAAC TGGAAGGACA TGGAAGTGTG TAAGTTACAG 
AGCATGGAGT CACAAGCCGA AAGGTTTCTC AAGAATCTGA AGCTAATGAG CAGTGATTCG 
GCAGCGTATT CTGACATGGA CTCAGACATA CATTCATGGG AATCGTTTCT TTCTGATGTT 
AGAGAAGAAA TTGAGAAAGC AAAGTCTCAG TTTGAAGAAC AAATTCAGGC AATTAAATAT 
GGTTCTCAGC TCAGTGAACT TCCTAAAGTG CAGATTTCTG AGCTTTCATT TCCTACCTGT 
AACACGATTC ATCCTAAATT ACTTCCTGAG TCTTCAGGCC AGGATGGTCA AGGTCCCTCC 
ACTTCTACAA GTCATGGGAC CGGAGACCAG ACAGCAGCTC CCGAGGAGTC AAGTCTGGGC 
TCTGCAGCTG ACAGCCCAGT GGAGTTGCCC TCCTGCAGTC AGAAAGCCCC CCAGCTGCCA 
GAGCATGAGG GTGCTGGCCA GGCAACTGGG CCAGAACCAG GCCCAGTGGC TGATCAGAAG 
TTACCTGCTG CTCCAGAGCG TGCCACTCAT TCAAGCCAGT CTCCAAAGAA GCCGTTCAAT 
AGCATTATTG AGCACCTGTT GGTGGTGTTC CCGTGTTACA GCAGCACGGA GCTTGCTGGT 
TTTATTAAAA AAGTTAGAAA CAAAAACAAG ACCTCTCTGT CTGGATTGAG TATTGACGAA 
ATTGTGCAAA GAGTGACAGA ACACGTCCTT GATGAAGAAA GAAAGAAAAA GCCAAGTCCA 
GGAAAGGGCT GGAGGCTGTC TGCACCCAGC TCTGCTGTTG CTGTGCCCAG GGCTGCCCAG 
GACCCGCCCT CCATGCCTGT CGGAGCATCG CCCGAAACCA GGGGGCCGAG GACCGAGGAC 
GTCCCTGTGT CCTGCGCAAA TTCCTGTGAA ATGTGCCGAG AAGTATTCAA GTCCAAACAC 
GTGCGTGAGC TGCAGTGTGG GCACAGGCTT CACAAGGGGT GCTTCAAGCA GTGGCTGAAG 
AGCCAGCGCA CCTGCCCAGC CTGCCTGGGT CACGACCTCC TGTCAGAGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_005202061.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005202118.1 Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X14, mRNA.

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CloneID	OBa218661
Clone ID Related Accession (Same CDS sequence)	XM_005202060.2 , XM_005202061.4 , XM_024999992.1
Accession Version	XM_005202061.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5034bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TTC3 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005202061.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGGGG AATATGACTG GGCCCTGCAA GCAAACATAA AAGCTCAAAA ACTCTGTAAA 
AATGACCCTG AGGGAATCAA GGATCTAATT CAGCAGCATG TAAAGTTACA AAAACAAATA 
GAAGACCTGC AAGGTCAAAC ACCAGCTAGT AATCCAGTCA AAGCCTTCTG TGAAAGCAGG 
GCCTACATAC CTAGTTTATC AGCACCTGCA TTTAGTACTT CACTTAACTT TGTGGAAACT 
GAAAAAAATT TCAGAAAAAC TAACCACGAA ATGGCAAACG GTGGTAATCA GAATCCAAAG 
GTGGTAGATG AAGCTCTGAA AGTAGATGAT TGTGACTGTC ATCCTGAATT TGTACCGCCA 
TCAAGTCAGC CTCCAAAATA TAAAGGGAAA CAAAAATCTC GAAACAATGA ATTGGAGAAG 
TTCAGCCCTG GTTCACAAGA AACTTTACCA GTAGATTTAA AAAACATCTT GGAGAAACAG 
TTTTCTAAAT CTTCCAGAGC CGCACACCAG GATTTTGCTA ATATAATGAA AATGTTGAGA 
AGTTTAATTC AGGATGGCTA CACGGCCTTG CTGGAGCAGC GCTGCCGCAG CGCCGCCCAA 
GCCTTCACAG AGTTGCTCAA CGGTTTAGAT CCTCAAAAAA TAAAGCAATT AAACCTGGCC 
ATGATTAACT ACGTGTTAGT AGTCTACGGA CTTGCCATTT CACTCCTTGG AATAGGACAG 
CCCGAGGAGT TATCTGAAGC TGAAAACCAG TTCAAGAGGA TGATTGAACA CTACCCCAAT 
GAGGGGCTTG ATTGCTTGGC CTACTGTGGA ATTGGAAAAG TGTATTTGAA AAAAAACAGA 
TTTTTAGAAG CTCTTAATCA TTTTGAGAAA GCAAGAACCT TGATCTGTCG TCTTCCTGGA 
GTGTTAACTT GGCCCACAAG TAATGTGGTT ATTGAGGAGA CTCAGCCAGG GAAGATAAAG 
ATGCTGTTAG AGAAATTTGT TGAAGAATGC AGGTTCCCTC CAGTGCCGGA TGCCACTTGT 
TGCTATCAGA AGTGCCATGG ATATTCCAAA ATCCAGATAT ATATAACTGA TCCAGACTTT 
AAGGGTTTTA TACGGATCAG CTGTTGCCAG TATTGTAAAA TAGAATTTCA CATGAACTGC 
TGGAAGAAGT TAAAAACAAC AACCTTCAAT GATAAAATTG ACAAGGATTT TCTGCAAGGA 
ATCTGTCTTA CTCCTGATTG TGAAGGAATC ATTTCAAAGA TTATCATCTT CAGCAGTGGT 
GGTCAAGTTA AATGTGAATT TGAACACAAG GTCATTAAAG AAAAGGTTCC TCCAAGACCT 
ATTCTGAAAC AGAAATGTTC TAGCCTAGAG AAGTTGAGGC TGAAAGAAGA CAAAAAATTG 
AAGCGAAAGC TCCAAAAAAA GGAAGCAAAA AAATTAGCAC AAGAAAGACT GGAAGAGGAC 
TTAAGGGAAA GTAACCCACC AAAAACTGAA GAGCAGAGAG AAACTATCAA CCATGTCCAA 
AGTTGCCAGT TCCTTGATGA CAGAATTCTG CAGTGCATAA AGCAGTACGC TGACAAGATT 
AAGTCCGGGA TACTGAATAC TTCCAAGCTT CTCAAAGAAT TGCTTTCTTG GAAGGTTTTA 
ACCACAGAAG ACTACACAAC GTGTTTTTCT AGCAGAAATT TCCTGAATGA AGCAGTGGAC 
TATGTCATTC GTCATTTGAT CCAAGAAAAG AATAGAGTAA AGACAAGGGT ATTTCTGCAT 
GTTTTGAGTG AGCTTAAAGA AGTGGACCCA AAACTAGCCA CCTGGATCCA GAAACTGAAT 
AGCTTTGGCT TAGATGCCAC AGGACCTTTC TTTTCTCGTT ATGGGGCTCC TCTTAAGGAG 
CTTGATTTTA GCATCATGAC TTTTCTCTGG AATGAGAAAT ACGGTCATAA GCTAGGCTCT 
ATAGAAGGAA AGCAACTTGA TTATTTCTAT GAGCCTACAT CACTGAAGGA AGCGCGGTGT 
TTAATATGGC TGCTAGAAGA ACACAGAGAC AAGTTCCCAG CACTGCATAA TGCCTTGGAC 
GAGTTCTTTG ATATAATGGA CAGCCGCTGC ACTGTATTAA GGAAACAGGA CAGTGGTGAC 
GTGCCATTTA ATTCTACCAA GATAAAAAAC AAAGGCAAGA AAAAGAAGCC AAAAGATTCA 
AAGCCTATGT TAGTTGGATC TGGAACACCT TCAGTCACAC CAAACAATGA GACTGTCACT 
TCAGGTGAAG ACCATATCAA ACGCAGTTTA AGTTCTGCGG GCCCCTTTGT AGTGCCTGAT 
CACCTTCGGC AGGATATAGA AGAATTTGAA GCTCTGTATG AACAGCATAG TAATGAGTAT 
GTTGTCCGTA ATAAGAAGCT GTGGGACATA AACCCAAAAC AGAAATGTTC AACTCTATAC 
GATTATTTCT CTCAGTTGTT GGAGGAACAT GGTCCCTTGG ACATGAGTAA CAAAATGTTT 
TCTGAAGAAT ATGAGTTCTT CCCAGAAGAA ACTCAACAGA TACTAGAAAA AGCAGGAGGC 
TTGAAAGCGT TTCTCCTGGG ATGTCCTCGT TTTGTTGTGA TTGATAACTG TATCGCATTG 
AAGAAAGTTG CATTACGGCT CAAGAAAAAG AGAAAGAAGA AAAACATTAA AACAAAAGTA 
GAAGAAATTT CAAAAACAGG AGAGTATTTA CGAGTTAAAC TACCACTGAA TCCAACTGCT 
AGGGAATTTA AACCAGATGT AAAGTCCAAA CCAGTGTCTG ACTTACCTTC AGCACCAGCT 
TCTGAGGACA TGAAGCCTCA GCCTGCACCT GCTAATTCTC CCCAATCAGC TCGTGACACT 
GGGAAGCCAA AACCAGTTTT CGATAATTCT TCCAGATCAG TTTCTGAGGA TGAGAAGCCC 
AAAGGGATGT CTTCTGATTC TCCCAGGCCA GTCTTTGAGG ATACCGGTCA CAAGCCGGTC 
TCCTCTAATT CTCCCAAACC AGTTCCTGAG GAAGTGAAAG CGACCTATTG GCCTCGACCC 
CAGTTGGTCA CAGGATACTG TACATATCTT CCCTTTCGGG GATTTGACAT TACCCAGACT 
CCACCAGCCT TCATCAGTGT GTTACCGACT TTGCCCCAGT ATAGCATTTA CACCCCTCTG 
GCCAGTGTTC CTACTGAGTA TCACCTGCAA AGACCAATAC CAGTGGTGCC GTCTTTTGTA 
GCCAGTGACA TAACGGAAGT GCATTTTGAG GCTCATCATT TCAGTGCTGA GAATGCCCCT 
GGTACCCAGA TTGCCTCCGA AACACAGATC CTTGAGGAAT CTTTGGAAAC ACCAGTGAAG 
TCAGAGTGCA GCTATGATGG TGCTGATATA GTCCAGAGTG AGGCTAGCAG AAACGATGAC 
TACTGTGGCG ATTCTGCCAA ATGGGAAGTA AATCCAGAAA GCACCAATGA AGTAACAAAT 
ATTCCATGTA CACAAGTGGT TGCTGTACAG GTGTCTTGGA ATATAACACA TCAGGAAGTC 
AATACTGAGC CATATGGTCC TTTTGAGGCA CAACAGGGGG GTATTTCACA GGTTGAAAAG 
GAGTACCAGG TTTTACAAGA GCAGCTTAAG GAAGCATGTG AAAATTATGA ACACAGCAAT 
GTCAAGGGCT CAGAAGAAAC CAAGGACTTG GAAGAAAAGT TGAAAAGGAA TGTTGAAGAA 
AATAAGATCT CAAAGACAGA ACTAGATTGG TTCCTTGAAG ATTTGAACAA GGAAATTAAA 
AAATGGGAAC AGGAGAAAAA AGAAATCCAA GAAAGACTAA AGGCACTGAA GAAGAAAATG 
AAAAAGGTGT TAAATGCCAG CAAAATGTAT ACGCAGGAAA ATGATGGAAA GGAAAAGGCA 
CTTGAATTAC TTCTGGATCA GTCCCTTGAA ATCAGTAATA CATTTACAAA TGAGAAAATG 
AAAATAGAAG AGAGTATAAA GAAAGGGAAA GAGCATTATG AAGAGAGTCT TCAAAGGGCT 
GTGGATGCAG AGGTATCTGT GCTTGAAAAC TGGAAGGACA TGGAAGTGTG TAAGTTACAG 
AGCATGGAGT CACAAGCCGA AAGGTTTCTC AAGAATCTGA AGCTAATGAG CAGTGATTCG 
GCAGCGTATT CTGACATGGA CTCAGACATA CATTCATGGG AATCGTTTCT TTCTGATGTT 
AGAGAAGAAA TTGAGAAAGC AAAGTCTCAG TTTGAAGAAC AAATTCAGGC AATTAAATAT 
GGTTCTCAGC TCAGTGAACT TCCTAAAGTG CAGATTTCTG AGCTTTCATT TCCTACCTGT 
AACACGATTC ATCCTAAATT ACTTCCTGAG TCTTCAGGCC AGGATGGTCA AGGTCCCTCC 
ACTTCTACAA GTCATGGGAC CGGAGACCAG ACAGCAGCTC CCGAGGAGTC AAGTCTGGGC 
TCTGCAGCTG ACAGCCCAGT GGAGTTGCCC TCCTGCAGTC AGAAAGCCCC CCAGCTGCCA 
GAGCATGAGG GTGCTGGCCA GGCAACTGGG CCAGAACCAG GCCCAGTGGC TGATCAGAAG 
TTACCTGCTG CTCCAGAGCG TGCCACTCAT TCAAGCCAGT CTCCAAAGAA GCCGTTCAAT 
AGCATTATTG AGCACCTGTT GGTGGTGTTC CCGTGTTACA GCAGCACGGA GCTTGCTGGT 
TTTATTAAAA AAGTTAGAAA CAAAAACAAG ACCTCTCTGT CTGGATTGAG TATTGACGAA 
ATTGTGCAAA GAGTGACAGA ACACGTCCTT GATGAAGAAA GAAAGAAAAA GCCAAGTCCA 
GGAAAGGGCT GGAGGCTGTC TGCACCCAGC TCTGCTGTTG CTGTGCCCAG GGCTGCCCAG 
GACCCGCCCT CCATGCCTGT CGGAGCATCG CCCGAAACCA GGGGGCCGAG GACCGAGGAC 
GTCCCTGTGT CCTGCGCAAA TTCCTGTGAA ATGTGCCGAG AAGTATTCAA GTCCAAACAC 
GTGCGTGAGC TGCAGTGTGG GCACAGGCTT CACAAGGGGT GCTTCAAGCA GTGGCTGAAG 
AGCCAGCGCA CCTGCCCAGC CTGCCTGGGT CACGACCTCC TGTCAGAGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005202118.1
CDS	416..5449
Translation	MLGEYDWALQANIKAQKLCKNDPEGIKDLIQQHVKLQKQIEDLQGQTPASNPVKAFCESRAYIPSLSAPAFSTSLNFVETEKNFRKTNHEMANGGNQNPKVVDEALKVDDCDCHPEFVPPSSQPPKYKGKQKSRNNELEKFSPGSQETLPVDLKNILEKQFSKSSRAAHQDFANIMKMLRSLIQDGYTALLEQRCRSAAQAFTELLNGLDPQKIKQLNLAMINYVLVVYGLAISLLGIGQPEELSEAENQFKRMIEHYPNEGLDCLAYCGIGKVYLKKNRFLEALNHFEKARTLICRLPGVLTWPTSNVVIEETQPGKIKMLLEKFVEECRFPPVPDATCCYQKCHGYSKIQIYITDPDFKGFIRISCCQYCKIEFHMNCWKKLKTTTFNDKIDKDFLQGICLTPDCEGIISKIIIFSSGGQVKCEFEHKVIKEKVPPRPILKQKCSSLEKLRLKEDKKLKRKLQKKEAKKLAQERLEEDLRESNPPKTEEQRETINHVQSCQFLDDRILQCIKQYADKIKSGILNTSKLLKELLSWKVLTTEDYTTCFSSRNFLNEAVDYVIRHLIQEKNRVKTRVFLHVLSELKEVDPKLATWIQKLNSFGLDATGPFFSRYGAPLKELDFSIMTFLWNEKYGHKLGSIEGKQLDYFYEPTSLKEARCLIWLLEEHRDKFPALHNALDEFFDIMDSRCTVLRKQDSGDVPFNSTKIKNKGKKKKPKDSKPMLVGSGTPSVTPNNETVTSGEDHIKRSLSSAGPFVVPDHLRQDIEEFEALYEQHSNEYVVRNKKLWDINPKQKCSTLYDYFSQLLEEHGPLDMSNKMFSEEYEFFPEETQQILEKAGGLKAFLLGCPRFVVIDNCIALKKVALRLKKKRKKKNIKTKVEEISKTGEYLRVKLPLNPTAREFKPDVKSKPVSDLPSAPASEDMKPQPAPANSPQSARDTGKPKPVFDNSSRSVSEDEKPKGMSSDSPRPVFEDTGHKPVSSNSPKPVPEEVKATYWPRPQLVTGYCTYLPFRGFDITQTPPAFISVLPTLPQYSIYTPLASVPTEYHLQRPIPVVPSFVASDITEVHFEAHHFSAENAPGTQIASETQILEESLETPVKSECSYDGADIVQSEASRNDDYCGDSAKWEVNPESTNEVTNIPCTQVVAVQVSWNITHQEVNTEPYGPFEAQQGGISQVEKEYQVLQEQLKEACENYEHSNVKGSEETKDLEEKLKRNVEENKISKTELDWFLEDLNKEIKKWEQEKKEIQERLKALKKKMKKVLNASKMYTQENDGKEKALELLLDQSLEISNTFTNEKMKIEESIKKGKEHYEESLQRAVDAEVSVLENWKDMEVCKLQSMESQAERFLKNLKLMSSDSAAYSDMDSDIHSWESFLSDVREEIEKAKSQFEEQIQAIKYGSQLSELPKVQISELSFPTCNTIHPKLLPESSGQDGQGPSTSTSHGTGDQTAAPEESSLGSAADSPVELPSCSQKAPQLPEHEGAGQATGPEPGPVADQKLPAAPERATHSSQSPKKPFNSIIEHLLVVFPCYSSTELAGFIKKVRNKNKTSLSGLSIDEIVQRVTEHVLDEERKKKPSPGKGWRLSAPSSAVAVPRAAQDPPSMPVGASPETRGPRTEDVPVSCANSCEMCREVFKSKHVRELQCGHRLHKGCFKQWLKSQRTCPACLGHDLLSEE

Target ORF information:

RefSeq Version	XM_005202061.4
Organism	Bos taurus(cattle)
Definition	Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005202061.4

ORF Insert Sequence:

ATGCTGGGGG AATATGACTG GGCCCTGCAA GCAAACATAA AAGCTCAAAA ACTCTGTAAA 
AATGACCCTG AGGGAATCAA GGATCTAATT CAGCAGCATG TAAAGTTACA AAAACAAATA 
GAAGACCTGC AAGGTCAAAC ACCAGCTAGT AATCCAGTCA AAGCCTTCTG TGAAAGCAGG 
GCCTACATAC CTAGTTTATC AGCACCTGCA TTTAGTACTT CACTTAACTT TGTGGAAACT 
GAAAAAAATT TCAGAAAAAC TAACCACGAA ATGGCAAACG GTGGTAATCA GAATCCAAAG 
GTGGTAGATG AAGCTCTGAA AGTAGATGAT TGTGACTGTC ATCCTGAATT TGTACCGCCA 
TCAAGTCAGC CTCCAAAATA TAAAGGGAAA CAAAAATCTC GAAACAATGA ATTGGAGAAG 
TTCAGCCCTG GTTCACAAGA AACTTTACCA GTAGATTTAA AAAACATCTT GGAGAAACAG 
TTTTCTAAAT CTTCCAGAGC CGCACACCAG GATTTTGCTA ATATAATGAA AATGTTGAGA 
AGTTTAATTC AGGATGGCTA CACGGCCTTG CTGGAGCAGC GCTGCCGCAG CGCCGCCCAA 
GCCTTCACAG AGTTGCTCAA CGGTTTAGAT CCTCAAAAAA TAAAGCAATT AAACCTGGCC 
ATGATTAACT ACGTGTTAGT AGTCTACGGA CTTGCCATTT CACTCCTTGG AATAGGACAG 
CCCGAGGAGT TATCTGAAGC TGAAAACCAG TTCAAGAGGA TGATTGAACA CTACCCCAAT 
GAGGGGCTTG ATTGCTTGGC CTACTGTGGA ATTGGAAAAG TGTATTTGAA AAAAAACAGA 
TTTTTAGAAG CTCTTAATCA TTTTGAGAAA GCAAGAACCT TGATCTGTCG TCTTCCTGGA 
GTGTTAACTT GGCCCACAAG TAATGTGGTT ATTGAGGAGA CTCAGCCAGG GAAGATAAAG 
ATGCTGTTAG AGAAATTTGT TGAAGAATGC AGGTTCCCTC CAGTGCCGGA TGCCACTTGT 
TGCTATCAGA AGTGCCATGG ATATTCCAAA ATCCAGATAT ATATAACTGA TCCAGACTTT 
AAGGGTTTTA TACGGATCAG CTGTTGCCAG TATTGTAAAA TAGAATTTCA CATGAACTGC 
TGGAAGAAGT TAAAAACAAC AACCTTCAAT GATAAAATTG ACAAGGATTT TCTGCAAGGA 
ATCTGTCTTA CTCCTGATTG TGAAGGAATC ATTTCAAAGA TTATCATCTT CAGCAGTGGT 
GGTCAAGTTA AATGTGAATT TGAACACAAG GTCATTAAAG AAAAGGTTCC TCCAAGACCT 
ATTCTGAAAC AGAAATGTTC TAGCCTAGAG AAGTTGAGGC TGAAAGAAGA CAAAAAATTG 
AAGCGAAAGC TCCAAAAAAA GGAAGCAAAA AAATTAGCAC AAGAAAGACT GGAAGAGGAC 
TTAAGGGAAA GTAACCCACC AAAAACTGAA GAGCAGAGAG AAACTATCAA CCATGTCCAA 
AGTTGCCAGT TCCTTGATGA CAGAATTCTG CAGTGCATAA AGCAGTACGC TGACAAGATT 
AAGTCCGGGA TACTGAATAC TTCCAAGCTT CTCAAAGAAT TGCTTTCTTG GAAGGTTTTA 
ACCACAGAAG ACTACACAAC GTGTTTTTCT AGCAGAAATT TCCTGAATGA AGCAGTGGAC 
TATGTCATTC GTCATTTGAT CCAAGAAAAG AATAGAGTAA AGACAAGGGT ATTTCTGCAT 
GTTTTGAGTG AGCTTAAAGA AGTGGACCCA AAACTAGCCA CCTGGATCCA GAAACTGAAT 
AGCTTTGGCT TAGATGCCAC AGGACCTTTC TTTTCTCGTT ATGGGGCTCC TCTTAAGGAG 
CTTGATTTTA GCATCATGAC TTTTCTCTGG AATGAGAAAT ACGGTCATAA GCTAGGCTCT 
ATAGAAGGAA AGCAACTTGA TTATTTCTAT GAGCCTACAT CACTGAAGGA AGCGCGGTGT 
TTAATATGGC TGCTAGAAGA ACACAGAGAC AAGTTCCCAG CACTGCATAA TGCCTTGGAC 
GAGTTCTTTG ATATAATGGA CAGCCGCTGC ACTGTATTAA GGAAACAGGA CAGTGGTGAC 
GTGCCATTTA ATTCTACCAA GATAAAAAAC AAAGGCAAGA AAAAGAAGCC AAAAGATTCA 
AAGCCTATGT TAGTTGGATC TGGAACACCT TCAGTCACAC CAAACAATGA GACTGTCACT 
TCAGGTGAAG ACCATATCAA ACGCAGTTTA AGTTCTGCGG GCCCCTTTGT AGTGCCTGAT 
CACCTTCGGC AGGATATAGA AGAATTTGAA GCTCTGTATG AACAGCATAG TAATGAGTAT 
GTTGTCCGTA ATAAGAAGCT GTGGGACATA AACCCAAAAC AGAAATGTTC AACTCTATAC 
GATTATTTCT CTCAGTTGTT GGAGGAACAT GGTCCCTTGG ACATGAGTAA CAAAATGTTT 
TCTGAAGAAT ATGAGTTCTT CCCAGAAGAA ACTCAACAGA TACTAGAAAA AGCAGGAGGC 
TTGAAAGCGT TTCTCCTGGG ATGTCCTCGT TTTGTTGTGA TTGATAACTG TATCGCATTG 
AAGAAAGTTG CATTACGGCT CAAGAAAAAG AGAAAGAAGA AAAACATTAA AACAAAAGTA 
GAAGAAATTT CAAAAACAGG AGAGTATTTA CGAGTTAAAC TACCACTGAA TCCAACTGCT 
AGGGAATTTA AACCAGATGT AAAGTCCAAA CCAGTGTCTG ACTTACCTTC AGCACCAGCT 
TCTGAGGACA TGAAGCCTCA GCCTGCACCT GCTAATTCTC CCCAATCAGC TCGTGACACT 
GGGAAGCCAA AACCAGTTTT CGATAATTCT TCCAGATCAG TTTCTGAGGA TGAGAAGCCC 
AAAGGGATGT CTTCTGATTC TCCCAGGCCA GTCTTTGAGG ATACCGGTCA CAAGCCGGTC 
TCCTCTAATT CTCCCAAACC AGTTCCTGAG GAAGTGAAAG CGACCTATTG GCCTCGACCC 
CAGTTGGTCA CAGGATACTG TACATATCTT CCCTTTCGGG GATTTGACAT TACCCAGACT 
CCACCAGCCT TCATCAGTGT GTTACCGACT TTGCCCCAGT ATAGCATTTA CACCCCTCTG 
GCCAGTGTTC CTACTGAGTA TCACCTGCAA AGACCAATAC CAGTGGTGCC GTCTTTTGTA 
GCCAGTGACA TAACGGAAGT GCATTTTGAG GCTCATCATT TCAGTGCTGA GAATGCCCCT 
GGTACCCAGA TTGCCTCCGA AACACAGATC CTTGAGGAAT CTTTGGAAAC ACCAGTGAAG 
TCAGAGTGCA GCTATGATGG TGCTGATATA GTCCAGAGTG AGGCTAGCAG AAACGATGAC 
TACTGTGGCG ATTCTGCCAA ATGGGAAGTA AATCCAGAAA GCACCAATGA AGTAACAAAT 
ATTCCATGTA CACAAGTGGT TGCTGTACAG GTGTCTTGGA ATATAACACA TCAGGAAGTC 
AATACTGAGC CATATGGTCC TTTTGAGGCA CAACAGGGGG GTATTTCACA GGTTGAAAAG 
GAGTACCAGG TTTTACAAGA GCAGCTTAAG GAAGCATGTG AAAATTATGA ACACAGCAAT 
GTCAAGGGCT CAGAAGAAAC CAAGGACTTG GAAGAAAAGT TGAAAAGGAA TGTTGAAGAA 
AATAAGATCT CAAAGACAGA ACTAGATTGG TTCCTTGAAG ATTTGAACAA GGAAATTAAA 
AAATGGGAAC AGGAGAAAAA AGAAATCCAA GAAAGACTAA AGGCACTGAA GAAGAAAATG 
AAAAAGGTGT TAAATGCCAG CAAAATGTAT ACGCAGGAAA ATGATGGAAA GGAAAAGGCA 
CTTGAATTAC TTCTGGATCA GTCCCTTGAA ATCAGTAATA CATTTACAAA TGAGAAAATG 
AAAATAGAAG AGAGTATAAA GAAAGGGAAA GAGCATTATG AAGAGAGTCT TCAAAGGGCT 
GTGGATGCAG AGGTATCTGT GCTTGAAAAC TGGAAGGACA TGGAAGTGTG TAAGTTACAG 
AGCATGGAGT CACAAGCCGA AAGGTTTCTC AAGAATCTGA AGCTAATGAG CAGTGATTCG 
GCAGCGTATT CTGACATGGA CTCAGACATA CATTCATGGG AATCGTTTCT TTCTGATGTT 
AGAGAAGAAA TTGAGAAAGC AAAGTCTCAG TTTGAAGAAC AAATTCAGGC AATTAAATAT 
GGTTCTCAGC TCAGTGAACT TCCTAAAGTG CAGATTTCTG AGCTTTCATT TCCTACCTGT 
AACACGATTC ATCCTAAATT ACTTCCTGAG TCTTCAGGCC AGGATGGTCA AGGTCCCTCC 
ACTTCTACAA GTCATGGGAC CGGAGACCAG ACAGCAGCTC CCGAGGAGTC AAGTCTGGGC 
TCTGCAGCTG ACAGCCCAGT GGAGTTGCCC TCCTGCAGTC AGAAAGCCCC CCAGCTGCCA 
GAGCATGAGG GTGCTGGCCA GGCAACTGGG CCAGAACCAG GCCCAGTGGC TGATCAGAAG 
TTACCTGCTG CTCCAGAGCG TGCCACTCAT TCAAGCCAGT CTCCAAAGAA GCCGTTCAAT 
AGCATTATTG AGCACCTGTT GGTGGTGTTC CCGTGTTACA GCAGCACGGA GCTTGCTGGT 
TTTATTAAAA AAGTTAGAAA CAAAAACAAG ACCTCTCTGT CTGGATTGAG TATTGACGAA 
ATTGTGCAAA GAGTGACAGA ACACGTCCTT GATGAAGAAA GAAAGAAAAA GCCAAGTCCA 
GGAAAGGGCT GGAGGCTGTC TGCACCCAGC TCTGCTGTTG CTGTGCCCAG GGCTGCCCAG 
GACCCGCCCT CCATGCCTGT CGGAGCATCG CCCGAAACCA GGGGGCCGAG GACCGAGGAC 
GTCCCTGTGT CCTGCGCAAA TTCCTGTGAA ATGTGCCGAG AAGTATTCAA GTCCAAACAC 
GTGCGTGAGC TGCAGTGTGG GCACAGGCTT CACAAGGGGT GCTTCAAGCA GTGGCTGAAG 
AGCCAGCGCA CCTGCCCAGC CTGCCTGGGT CACGACCTCC TGTCAGAGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_024999992.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855760.1 Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X13, mRNA.

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CloneID	OBa218661
Clone ID Related Accession (Same CDS sequence)	XM_005202060.2 , XM_005202061.4 , XM_024999992.1
Accession Version	XM_024999992.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5034bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TTC3 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGGGG AATATGACTG GGCCCTGCAA GCAAACATAA AAGCTCAAAA ACTCTGTAAA 
AATGACCCTG AGGGAATCAA GGATCTAATT CAGCAGCATG TAAAGTTACA AAAACAAATA 
GAAGACCTGC AAGGTCAAAC ACCAGCTAGT AATCCAGTCA AAGCCTTCTG TGAAAGCAGG 
GCCTACATAC CTAGTTTATC AGCACCTGCA TTTAGTACTT CACTTAACTT TGTGGAAACT 
GAAAAAAATT TCAGAAAAAC TAACCACGAA ATGGCAAACG GTGGTAATCA GAATCCAAAG 
GTGGTAGATG AAGCTCTGAA AGTAGATGAT TGTGACTGTC ATCCTGAATT TGTACCGCCA 
TCAAGTCAGC CTCCAAAATA TAAAGGGAAA CAAAAATCTC GAAACAATGA ATTGGAGAAG 
TTCAGCCCTG GTTCACAAGA AACTTTACCA GTAGATTTAA AAAACATCTT GGAGAAACAG 
TTTTCTAAAT CTTCCAGAGC CGCACACCAG GATTTTGCTA ATATAATGAA AATGTTGAGA 
AGTTTAATTC AGGATGGCTA CACGGCCTTG CTGGAGCAGC GCTGCCGCAG CGCCGCCCAA 
GCCTTCACAG AGTTGCTCAA CGGTTTAGAT CCTCAAAAAA TAAAGCAATT AAACCTGGCC 
ATGATTAACT ACGTGTTAGT AGTCTACGGA CTTGCCATTT CACTCCTTGG AATAGGACAG 
CCCGAGGAGT TATCTGAAGC TGAAAACCAG TTCAAGAGGA TGATTGAACA CTACCCCAAT 
GAGGGGCTTG ATTGCTTGGC CTACTGTGGA ATTGGAAAAG TGTATTTGAA AAAAAACAGA 
TTTTTAGAAG CTCTTAATCA TTTTGAGAAA GCAAGAACCT TGATCTGTCG TCTTCCTGGA 
GTGTTAACTT GGCCCACAAG TAATGTGGTT ATTGAGGAGA CTCAGCCAGG GAAGATAAAG 
ATGCTGTTAG AGAAATTTGT TGAAGAATGC AGGTTCCCTC CAGTGCCGGA TGCCACTTGT 
TGCTATCAGA AGTGCCATGG ATATTCCAAA ATCCAGATAT ATATAACTGA TCCAGACTTT 
AAGGGTTTTA TACGGATCAG CTGTTGCCAG TATTGTAAAA TAGAATTTCA CATGAACTGC 
TGGAAGAAGT TAAAAACAAC AACCTTCAAT GATAAAATTG ACAAGGATTT TCTGCAAGGA 
ATCTGTCTTA CTCCTGATTG TGAAGGAATC ATTTCAAAGA TTATCATCTT CAGCAGTGGT 
GGTCAAGTTA AATGTGAATT TGAACACAAG GTCATTAAAG AAAAGGTTCC TCCAAGACCT 
ATTCTGAAAC AGAAATGTTC TAGCCTAGAG AAGTTGAGGC TGAAAGAAGA CAAAAAATTG 
AAGCGAAAGC TCCAAAAAAA GGAAGCAAAA AAATTAGCAC AAGAAAGACT GGAAGAGGAC 
TTAAGGGAAA GTAACCCACC AAAAACTGAA GAGCAGAGAG AAACTATCAA CCATGTCCAA 
AGTTGCCAGT TCCTTGATGA CAGAATTCTG CAGTGCATAA AGCAGTACGC TGACAAGATT 
AAGTCCGGGA TACTGAATAC TTCCAAGCTT CTCAAAGAAT TGCTTTCTTG GAAGGTTTTA 
ACCACAGAAG ACTACACAAC GTGTTTTTCT AGCAGAAATT TCCTGAATGA AGCAGTGGAC 
TATGTCATTC GTCATTTGAT CCAAGAAAAG AATAGAGTAA AGACAAGGGT ATTTCTGCAT 
GTTTTGAGTG AGCTTAAAGA AGTGGACCCA AAACTAGCCA CCTGGATCCA GAAACTGAAT 
AGCTTTGGCT TAGATGCCAC AGGACCTTTC TTTTCTCGTT ATGGGGCTCC TCTTAAGGAG 
CTTGATTTTA GCATCATGAC TTTTCTCTGG AATGAGAAAT ACGGTCATAA GCTAGGCTCT 
ATAGAAGGAA AGCAACTTGA TTATTTCTAT GAGCCTACAT CACTGAAGGA AGCGCGGTGT 
TTAATATGGC TGCTAGAAGA ACACAGAGAC AAGTTCCCAG CACTGCATAA TGCCTTGGAC 
GAGTTCTTTG ATATAATGGA CAGCCGCTGC ACTGTATTAA GGAAACAGGA CAGTGGTGAC 
GTGCCATTTA ATTCTACCAA GATAAAAAAC AAAGGCAAGA AAAAGAAGCC AAAAGATTCA 
AAGCCTATGT TAGTTGGATC TGGAACACCT TCAGTCACAC CAAACAATGA GACTGTCACT 
TCAGGTGAAG ACCATATCAA ACGCAGTTTA AGTTCTGCGG GCCCCTTTGT AGTGCCTGAT 
CACCTTCGGC AGGATATAGA AGAATTTGAA GCTCTGTATG AACAGCATAG TAATGAGTAT 
GTTGTCCGTA ATAAGAAGCT GTGGGACATA AACCCAAAAC AGAAATGTTC AACTCTATAC 
GATTATTTCT CTCAGTTGTT GGAGGAACAT GGTCCCTTGG ACATGAGTAA CAAAATGTTT 
TCTGAAGAAT ATGAGTTCTT CCCAGAAGAA ACTCAACAGA TACTAGAAAA AGCAGGAGGC 
TTGAAAGCGT TTCTCCTGGG ATGTCCTCGT TTTGTTGTGA TTGATAACTG TATCGCATTG 
AAGAAAGTTG CATTACGGCT CAAGAAAAAG AGAAAGAAGA AAAACATTAA AACAAAAGTA 
GAAGAAATTT CAAAAACAGG AGAGTATTTA CGAGTTAAAC TACCACTGAA TCCAACTGCT 
AGGGAATTTA AACCAGATGT AAAGTCCAAA CCAGTGTCTG ACTTACCTTC AGCACCAGCT 
TCTGAGGACA TGAAGCCTCA GCCTGCACCT GCTAATTCTC CCCAATCAGC TCGTGACACT 
GGGAAGCCAA AACCAGTTTT CGATAATTCT TCCAGATCAG TTTCTGAGGA TGAGAAGCCC 
AAAGGGATGT CTTCTGATTC TCCCAGGCCA GTCTTTGAGG ATACCGGTCA CAAGCCGGTC 
TCCTCTAATT CTCCCAAACC AGTTCCTGAG GAAGTGAAAG CGACCTATTG GCCTCGACCC 
CAGTTGGTCA CAGGATACTG TACATATCTT CCCTTTCGGG GATTTGACAT TACCCAGACT 
CCACCAGCCT TCATCAGTGT GTTACCGACT TTGCCCCAGT ATAGCATTTA CACCCCTCTG 
GCCAGTGTTC CTACTGAGTA TCACCTGCAA AGACCAATAC CAGTGGTGCC GTCTTTTGTA 
GCCAGTGACA TAACGGAAGT GCATTTTGAG GCTCATCATT TCAGTGCTGA GAATGCCCCT 
GGTACCCAGA TTGCCTCCGA AACACAGATC CTTGAGGAAT CTTTGGAAAC ACCAGTGAAG 
TCAGAGTGCA GCTATGATGG TGCTGATATA GTCCAGAGTG AGGCTAGCAG AAACGATGAC 
TACTGTGGCG ATTCTGCCAA ATGGGAAGTA AATCCAGAAA GCACCAATGA AGTAACAAAT 
ATTCCATGTA CACAAGTGGT TGCTGTACAG GTGTCTTGGA ATATAACACA TCAGGAAGTC 
AATACTGAGC CATATGGTCC TTTTGAGGCA CAACAGGGGG GTATTTCACA GGTTGAAAAG 
GAGTACCAGG TTTTACAAGA GCAGCTTAAG GAAGCATGTG AAAATTATGA ACACAGCAAT 
GTCAAGGGCT CAGAAGAAAC CAAGGACTTG GAAGAAAAGT TGAAAAGGAA TGTTGAAGAA 
AATAAGATCT CAAAGACAGA ACTAGATTGG TTCCTTGAAG ATTTGAACAA GGAAATTAAA 
AAATGGGAAC AGGAGAAAAA AGAAATCCAA GAAAGACTAA AGGCACTGAA GAAGAAAATG 
AAAAAGGTGT TAAATGCCAG CAAAATGTAT ACGCAGGAAA ATGATGGAAA GGAAAAGGCA 
CTTGAATTAC TTCTGGATCA GTCCCTTGAA ATCAGTAATA CATTTACAAA TGAGAAAATG 
AAAATAGAAG AGAGTATAAA GAAAGGGAAA GAGCATTATG AAGAGAGTCT TCAAAGGGCT 
GTGGATGCAG AGGTATCTGT GCTTGAAAAC TGGAAGGACA TGGAAGTGTG TAAGTTACAG 
AGCATGGAGT CACAAGCCGA AAGGTTTCTC AAGAATCTGA AGCTAATGAG CAGTGATTCG 
GCAGCGTATT CTGACATGGA CTCAGACATA CATTCATGGG AATCGTTTCT TTCTGATGTT 
AGAGAAGAAA TTGAGAAAGC AAAGTCTCAG TTTGAAGAAC AAATTCAGGC AATTAAATAT 
GGTTCTCAGC TCAGTGAACT TCCTAAAGTG CAGATTTCTG AGCTTTCATT TCCTACCTGT 
AACACGATTC ATCCTAAATT ACTTCCTGAG TCTTCAGGCC AGGATGGTCA AGGTCCCTCC 
ACTTCTACAA GTCATGGGAC CGGAGACCAG ACAGCAGCTC CCGAGGAGTC AAGTCTGGGC 
TCTGCAGCTG ACAGCCCAGT GGAGTTGCCC TCCTGCAGTC AGAAAGCCCC CCAGCTGCCA 
GAGCATGAGG GTGCTGGCCA GGCAACTGGG CCAGAACCAG GCCCAGTGGC TGATCAGAAG 
TTACCTGCTG CTCCAGAGCG TGCCACTCAT TCAAGCCAGT CTCCAAAGAA GCCGTTCAAT 
AGCATTATTG AGCACCTGTT GGTGGTGTTC CCGTGTTACA GCAGCACGGA GCTTGCTGGT 
TTTATTAAAA AAGTTAGAAA CAAAAACAAG ACCTCTCTGT CTGGATTGAG TATTGACGAA 
ATTGTGCAAA GAGTGACAGA ACACGTCCTT GATGAAGAAA GAAAGAAAAA GCCAAGTCCA 
GGAAAGGGCT GGAGGCTGTC TGCACCCAGC TCTGCTGTTG CTGTGCCCAG GGCTGCCCAG 
GACCCGCCCT CCATGCCTGT CGGAGCATCG CCCGAAACCA GGGGGCCGAG GACCGAGGAC 
GTCCCTGTGT CCTGCGCAAA TTCCTGTGAA ATGTGCCGAG AAGTATTCAA GTCCAAACAC 
GTGCGTGAGC TGCAGTGTGG GCACAGGCTT CACAAGGGGT GCTTCAAGCA GTGGCTGAAG 
AGCCAGCGCA CCTGCCCAGC CTGCCTGGGT CACGACCTCC TGTCAGAGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855760.1
CDS	179..5212
Translation	MLGEYDWALQANIKAQKLCKNDPEGIKDLIQQHVKLQKQIEDLQGQTPASNPVKAFCESRAYIPSLSAPAFSTSLNFVETEKNFRKTNHEMANGGNQNPKVVDEALKVDDCDCHPEFVPPSSQPPKYKGKQKSRNNELEKFSPGSQETLPVDLKNILEKQFSKSSRAAHQDFANIMKMLRSLIQDGYTALLEQRCRSAAQAFTELLNGLDPQKIKQLNLAMINYVLVVYGLAISLLGIGQPEELSEAENQFKRMIEHYPNEGLDCLAYCGIGKVYLKKNRFLEALNHFEKARTLICRLPGVLTWPTSNVVIEETQPGKIKMLLEKFVEECRFPPVPDATCCYQKCHGYSKIQIYITDPDFKGFIRISCCQYCKIEFHMNCWKKLKTTTFNDKIDKDFLQGICLTPDCEGIISKIIIFSSGGQVKCEFEHKVIKEKVPPRPILKQKCSSLEKLRLKEDKKLKRKLQKKEAKKLAQERLEEDLRESNPPKTEEQRETINHVQSCQFLDDRILQCIKQYADKIKSGILNTSKLLKELLSWKVLTTEDYTTCFSSRNFLNEAVDYVIRHLIQEKNRVKTRVFLHVLSELKEVDPKLATWIQKLNSFGLDATGPFFSRYGAPLKELDFSIMTFLWNEKYGHKLGSIEGKQLDYFYEPTSLKEARCLIWLLEEHRDKFPALHNALDEFFDIMDSRCTVLRKQDSGDVPFNSTKIKNKGKKKKPKDSKPMLVGSGTPSVTPNNETVTSGEDHIKRSLSSAGPFVVPDHLRQDIEEFEALYEQHSNEYVVRNKKLWDINPKQKCSTLYDYFSQLLEEHGPLDMSNKMFSEEYEFFPEETQQILEKAGGLKAFLLGCPRFVVIDNCIALKKVALRLKKKRKKKNIKTKVEEISKTGEYLRVKLPLNPTAREFKPDVKSKPVSDLPSAPASEDMKPQPAPANSPQSARDTGKPKPVFDNSSRSVSEDEKPKGMSSDSPRPVFEDTGHKPVSSNSPKPVPEEVKATYWPRPQLVTGYCTYLPFRGFDITQTPPAFISVLPTLPQYSIYTPLASVPTEYHLQRPIPVVPSFVASDITEVHFEAHHFSAENAPGTQIASETQILEESLETPVKSECSYDGADIVQSEASRNDDYCGDSAKWEVNPESTNEVTNIPCTQVVAVQVSWNITHQEVNTEPYGPFEAQQGGISQVEKEYQVLQEQLKEACENYEHSNVKGSEETKDLEEKLKRNVEENKISKTELDWFLEDLNKEIKKWEQEKKEIQERLKALKKKMKKVLNASKMYTQENDGKEKALELLLDQSLEISNTFTNEKMKIEESIKKGKEHYEESLQRAVDAEVSVLENWKDMEVCKLQSMESQAERFLKNLKLMSSDSAAYSDMDSDIHSWESFLSDVREEIEKAKSQFEEQIQAIKYGSQLSELPKVQISELSFPTCNTIHPKLLPESSGQDGQGPSTSTSHGTGDQTAAPEESSLGSAADSPVELPSCSQKAPQLPEHEGAGQATGPEPGPVADQKLPAAPERATHSSQSPKKPFNSIIEHLLVVFPCYSSTELAGFIKKVRNKNKTSLSGLSIDEIVQRVTEHVLDEERKKKPSPGKGWRLSAPSSAVAVPRAAQDPPSMPVGASPETRGPRTEDVPVSCANSCEMCREVFKSKHVRELQCGHRLHKGCFKQWLKSQRTCPACLGHDLLSEE

Target ORF information:

RefSeq Version	XM_024999992.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999992.1

ORF Insert Sequence:

ATGCTGGGGG AATATGACTG GGCCCTGCAA GCAAACATAA AAGCTCAAAA ACTCTGTAAA 
AATGACCCTG AGGGAATCAA GGATCTAATT CAGCAGCATG TAAAGTTACA AAAACAAATA 
GAAGACCTGC AAGGTCAAAC ACCAGCTAGT AATCCAGTCA AAGCCTTCTG TGAAAGCAGG 
GCCTACATAC CTAGTTTATC AGCACCTGCA TTTAGTACTT CACTTAACTT TGTGGAAACT 
GAAAAAAATT TCAGAAAAAC TAACCACGAA ATGGCAAACG GTGGTAATCA GAATCCAAAG 
GTGGTAGATG AAGCTCTGAA AGTAGATGAT TGTGACTGTC ATCCTGAATT TGTACCGCCA 
TCAAGTCAGC CTCCAAAATA TAAAGGGAAA CAAAAATCTC GAAACAATGA ATTGGAGAAG 
TTCAGCCCTG GTTCACAAGA AACTTTACCA GTAGATTTAA AAAACATCTT GGAGAAACAG 
TTTTCTAAAT CTTCCAGAGC CGCACACCAG GATTTTGCTA ATATAATGAA AATGTTGAGA 
AGTTTAATTC AGGATGGCTA CACGGCCTTG CTGGAGCAGC GCTGCCGCAG CGCCGCCCAA 
GCCTTCACAG AGTTGCTCAA CGGTTTAGAT CCTCAAAAAA TAAAGCAATT AAACCTGGCC 
ATGATTAACT ACGTGTTAGT AGTCTACGGA CTTGCCATTT CACTCCTTGG AATAGGACAG 
CCCGAGGAGT TATCTGAAGC TGAAAACCAG TTCAAGAGGA TGATTGAACA CTACCCCAAT 
GAGGGGCTTG ATTGCTTGGC CTACTGTGGA ATTGGAAAAG TGTATTTGAA AAAAAACAGA 
TTTTTAGAAG CTCTTAATCA TTTTGAGAAA GCAAGAACCT TGATCTGTCG TCTTCCTGGA 
GTGTTAACTT GGCCCACAAG TAATGTGGTT ATTGAGGAGA CTCAGCCAGG GAAGATAAAG 
ATGCTGTTAG AGAAATTTGT TGAAGAATGC AGGTTCCCTC CAGTGCCGGA TGCCACTTGT 
TGCTATCAGA AGTGCCATGG ATATTCCAAA ATCCAGATAT ATATAACTGA TCCAGACTTT 
AAGGGTTTTA TACGGATCAG CTGTTGCCAG TATTGTAAAA TAGAATTTCA CATGAACTGC 
TGGAAGAAGT TAAAAACAAC AACCTTCAAT GATAAAATTG ACAAGGATTT TCTGCAAGGA 
ATCTGTCTTA CTCCTGATTG TGAAGGAATC ATTTCAAAGA TTATCATCTT CAGCAGTGGT 
GGTCAAGTTA AATGTGAATT TGAACACAAG GTCATTAAAG AAAAGGTTCC TCCAAGACCT 
ATTCTGAAAC AGAAATGTTC TAGCCTAGAG AAGTTGAGGC TGAAAGAAGA CAAAAAATTG 
AAGCGAAAGC TCCAAAAAAA GGAAGCAAAA AAATTAGCAC AAGAAAGACT GGAAGAGGAC 
TTAAGGGAAA GTAACCCACC AAAAACTGAA GAGCAGAGAG AAACTATCAA CCATGTCCAA 
AGTTGCCAGT TCCTTGATGA CAGAATTCTG CAGTGCATAA AGCAGTACGC TGACAAGATT 
AAGTCCGGGA TACTGAATAC TTCCAAGCTT CTCAAAGAAT TGCTTTCTTG GAAGGTTTTA 
ACCACAGAAG ACTACACAAC GTGTTTTTCT AGCAGAAATT TCCTGAATGA AGCAGTGGAC 
TATGTCATTC GTCATTTGAT CCAAGAAAAG AATAGAGTAA AGACAAGGGT ATTTCTGCAT 
GTTTTGAGTG AGCTTAAAGA AGTGGACCCA AAACTAGCCA CCTGGATCCA GAAACTGAAT 
AGCTTTGGCT TAGATGCCAC AGGACCTTTC TTTTCTCGTT ATGGGGCTCC TCTTAAGGAG 
CTTGATTTTA GCATCATGAC TTTTCTCTGG AATGAGAAAT ACGGTCATAA GCTAGGCTCT 
ATAGAAGGAA AGCAACTTGA TTATTTCTAT GAGCCTACAT CACTGAAGGA AGCGCGGTGT 
TTAATATGGC TGCTAGAAGA ACACAGAGAC AAGTTCCCAG CACTGCATAA TGCCTTGGAC 
GAGTTCTTTG ATATAATGGA CAGCCGCTGC ACTGTATTAA GGAAACAGGA CAGTGGTGAC 
GTGCCATTTA ATTCTACCAA GATAAAAAAC AAAGGCAAGA AAAAGAAGCC AAAAGATTCA 
AAGCCTATGT TAGTTGGATC TGGAACACCT TCAGTCACAC CAAACAATGA GACTGTCACT 
TCAGGTGAAG ACCATATCAA ACGCAGTTTA AGTTCTGCGG GCCCCTTTGT AGTGCCTGAT 
CACCTTCGGC AGGATATAGA AGAATTTGAA GCTCTGTATG AACAGCATAG TAATGAGTAT 
GTTGTCCGTA ATAAGAAGCT GTGGGACATA AACCCAAAAC AGAAATGTTC AACTCTATAC 
GATTATTTCT CTCAGTTGTT GGAGGAACAT GGTCCCTTGG ACATGAGTAA CAAAATGTTT 
TCTGAAGAAT ATGAGTTCTT CCCAGAAGAA ACTCAACAGA TACTAGAAAA AGCAGGAGGC 
TTGAAAGCGT TTCTCCTGGG ATGTCCTCGT TTTGTTGTGA TTGATAACTG TATCGCATTG 
AAGAAAGTTG CATTACGGCT CAAGAAAAAG AGAAAGAAGA AAAACATTAA AACAAAAGTA 
GAAGAAATTT CAAAAACAGG AGAGTATTTA CGAGTTAAAC TACCACTGAA TCCAACTGCT 
AGGGAATTTA AACCAGATGT AAAGTCCAAA CCAGTGTCTG ACTTACCTTC AGCACCAGCT 
TCTGAGGACA TGAAGCCTCA GCCTGCACCT GCTAATTCTC CCCAATCAGC TCGTGACACT 
GGGAAGCCAA AACCAGTTTT CGATAATTCT TCCAGATCAG TTTCTGAGGA TGAGAAGCCC 
AAAGGGATGT CTTCTGATTC TCCCAGGCCA GTCTTTGAGG ATACCGGTCA CAAGCCGGTC 
TCCTCTAATT CTCCCAAACC AGTTCCTGAG GAAGTGAAAG CGACCTATTG GCCTCGACCC 
CAGTTGGTCA CAGGATACTG TACATATCTT CCCTTTCGGG GATTTGACAT TACCCAGACT 
CCACCAGCCT TCATCAGTGT GTTACCGACT TTGCCCCAGT ATAGCATTTA CACCCCTCTG 
GCCAGTGTTC CTACTGAGTA TCACCTGCAA AGACCAATAC CAGTGGTGCC GTCTTTTGTA 
GCCAGTGACA TAACGGAAGT GCATTTTGAG GCTCATCATT TCAGTGCTGA GAATGCCCCT 
GGTACCCAGA TTGCCTCCGA AACACAGATC CTTGAGGAAT CTTTGGAAAC ACCAGTGAAG 
TCAGAGTGCA GCTATGATGG TGCTGATATA GTCCAGAGTG AGGCTAGCAG AAACGATGAC 
TACTGTGGCG ATTCTGCCAA ATGGGAAGTA AATCCAGAAA GCACCAATGA AGTAACAAAT 
ATTCCATGTA CACAAGTGGT TGCTGTACAG GTGTCTTGGA ATATAACACA TCAGGAAGTC 
AATACTGAGC CATATGGTCC TTTTGAGGCA CAACAGGGGG GTATTTCACA GGTTGAAAAG 
GAGTACCAGG TTTTACAAGA GCAGCTTAAG GAAGCATGTG AAAATTATGA ACACAGCAAT 
GTCAAGGGCT CAGAAGAAAC CAAGGACTTG GAAGAAAAGT TGAAAAGGAA TGTTGAAGAA 
AATAAGATCT CAAAGACAGA ACTAGATTGG TTCCTTGAAG ATTTGAACAA GGAAATTAAA 
AAATGGGAAC AGGAGAAAAA AGAAATCCAA GAAAGACTAA AGGCACTGAA GAAGAAAATG 
AAAAAGGTGT TAAATGCCAG CAAAATGTAT ACGCAGGAAA ATGATGGAAA GGAAAAGGCA 
CTTGAATTAC TTCTGGATCA GTCCCTTGAA ATCAGTAATA CATTTACAAA TGAGAAAATG 
AAAATAGAAG AGAGTATAAA GAAAGGGAAA GAGCATTATG AAGAGAGTCT TCAAAGGGCT 
GTGGATGCAG AGGTATCTGT GCTTGAAAAC TGGAAGGACA TGGAAGTGTG TAAGTTACAG 
AGCATGGAGT CACAAGCCGA AAGGTTTCTC AAGAATCTGA AGCTAATGAG CAGTGATTCG 
GCAGCGTATT CTGACATGGA CTCAGACATA CATTCATGGG AATCGTTTCT TTCTGATGTT 
AGAGAAGAAA TTGAGAAAGC AAAGTCTCAG TTTGAAGAAC AAATTCAGGC AATTAAATAT 
GGTTCTCAGC TCAGTGAACT TCCTAAAGTG CAGATTTCTG AGCTTTCATT TCCTACCTGT 
AACACGATTC ATCCTAAATT ACTTCCTGAG TCTTCAGGCC AGGATGGTCA AGGTCCCTCC 
ACTTCTACAA GTCATGGGAC CGGAGACCAG ACAGCAGCTC CCGAGGAGTC AAGTCTGGGC 
TCTGCAGCTG ACAGCCCAGT GGAGTTGCCC TCCTGCAGTC AGAAAGCCCC CCAGCTGCCA 
GAGCATGAGG GTGCTGGCCA GGCAACTGGG CCAGAACCAG GCCCAGTGGC TGATCAGAAG 
TTACCTGCTG CTCCAGAGCG TGCCACTCAT TCAAGCCAGT CTCCAAAGAA GCCGTTCAAT 
AGCATTATTG AGCACCTGTT GGTGGTGTTC CCGTGTTACA GCAGCACGGA GCTTGCTGGT 
TTTATTAAAA AAGTTAGAAA CAAAAACAAG ACCTCTCTGT CTGGATTGAG TATTGACGAA 
ATTGTGCAAA GAGTGACAGA ACACGTCCTT GATGAAGAAA GAAAGAAAAA GCCAAGTCCA 
GGAAAGGGCT GGAGGCTGTC TGCACCCAGC TCTGCTGTTG CTGTGCCCAG GGCTGCCCAG 
GACCCGCCCT CCATGCCTGT CGGAGCATCG CCCGAAACCA GGGGGCCGAG GACCGAGGAC 
GTCCCTGTGT CCTGCGCAAA TTCCTGTGAA ATGTGCCGAG AAGTATTCAA GTCCAAACAC 
GTGCGTGAGC TGCAGTGTGG GCACAGGCTT CACAAGGGGT GCTTCAAGCA GTGGCTGAAG 
AGCCAGCGCA CCTGCCCAGC CTGCCTGGGT CACGACCTCC TGTCAGAGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( NM_001109767.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001103237.2 Bos taurus tetratricopeptide repeat domain 3 (TTC3), mRNA.

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CloneID	OBa16127
Clone ID Related Accession (Same CDS sequence)	XM_005202058.3 , XM_005202057.3 , NM_001109767.3 , XM_005202056.3
Accession Version	NM_001109767.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5958bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TTC3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DAAA02003579.1 and DAAA02003580.1. On Oct 1, 2015 this sequence version replaced NM_001109767.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments and orthologs. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

ATGGCCGATT TTCCCCAGGG AAATTTTGCA GATTATGCCT TGTTAGAAGA TTGCCCTTAT 
GTGGATGATT GTATCTTTGC CCCTGAGTTT ATGTCCAGTG ATTATGTTCG TGTGACTCAG 
CTTTACTGTG ATGGAGTGGG TAGACAGTAT AAAGATTATG CCCAAACTGA GGGAAACTTA 
GAATTTGACA TCTGCACTCT GTGGTGTAGT CAACCTATTT CTGTCCTGCA AGATTACTGT 
GATGCCATTA AAGTCCACAT CTTCTGGCCA CTTCTGTTTC AACGTCAGCA GAGCTCTATA 
ATAACACGGA TGCACTCCTG TGTAAGGACC AGCGGTTCTC ATGCTTGTGA GATAAGTTTG 
AAGAAATTAC AGCAGCTTGA ACTGATGGAA GATATTGTGG ATTTGGCAAA GAAAGTTGTG 
AATGATACAT TCTTTATCGG AGGCTTATTG AGAATTGGCT ATAAAATAGA GAATAAAATC 
TTGGCAATGG AAGAAGCTTT GAATTGGGTG AAATACACAG GCGATGTAAC AATTTTATCT 
AAATTAGGAG CAGTTGATAA TTGTTGGCGT ATGTTAAGTA TTTTCTTTAC TGAATACAAG 
TATCACATAA CTAAAGTTGT AACAGAAAAC TGCAGTTTGC TTGAAGAATT TAAAACTCAA 
AGTTGTGCGG AGTGTCTAGA GCAAGGAGAA CTAATGAAAA TGAAAGGAAA TGAAGAGTTT 
TCCAAAGAAA GATATGATAT AGCTATTATC TATTACACCA GAGCCATTGA ATGTAGGCCT 
GAAAATCACC TGCTTTATGG TAACCGAGCT CTTTGTTTTC TTCGTACTGG ACAATTCAGA 
AATGCACTTG GTGATGGAAA GAGAGCTACC ATTCTGAAGA ACAATTGGAC AAAGGGTCAT 
TATCGTTATT GTGCTGCTCT TTCTATGCTG GGGGAATATG ACTGGGCCCT GCAAGCAAAC 
ATAAAAGCTC AAAAACTCTG TAAAAATGAC CCTGAGGGAA TCAAGGATCT AATTCAGCAG 
CATGTAAAGT TACAAAAACA AATAGAAGAC CTGCAAGGTC AAACACCAGC TAGTAATCCA 
GTCAAAGCCT TCTGTGAAAG CAGGGCCTAC ATACCTAGTT TATCAGCACC TGCATTTAGT 
ACTTCACTTA ACTTTGTGGA AACTGAAAAA AATTTCAGAA AAACTAACCA CGAAATGGCA 
AACGGTGGTA ATCAGAATCC AAAGGTGGTA GATGAAGCTC TGAAAGTAGA TGATTGTGAC 
TGTCATCCTG AATTTGTACC GCCATCAAGT CAGCCTCCAA AATATAAAGG GAAACAAAAA 
TCTCGAAACA ATGAATTGGA GAAGTTCAGC CCTGGTTCAC AAGAAACTTT ACCAGTAGAT 
TTAAAAAACA TCTTGGAGAA ACAGTTTTCT AAATCTTCCA GAGCCGCACA CCAGGATTTT 
GCTAATATAA TGAAAATGCT GAGAAGTTTA ATTCAGGATG GCTACACGGC CTTGCTGGAG 
CAGCGCTGCC GCAGCGCCGC CCAAGCCTTC ACAGAGTTGC TCAACGGTTT AGATCCTCAA 
AAAATAAAGC AATTGAACCT GGCCATGATT AACTATGTGT TAGTAGTCTA CGGACTTGCC 
ATTTCACTCC TTGGAATAGG ACAGCCCGAG GAGTTATCTG AAGCTGAAAA CCAGTTCAAG 
AGGATGATTG AACACTACCC CAATGAGGGG CTTGATTGCT TGGCCTACTG TGGAATTGGA 
AAAGTGTATT TGAAAAAAAA CAGATTTTTA GAAGCTCTTA ATCATTTTGA GAAAGCAAGA 
ACCTTGATCT GCCGTCTTCC TGGAGTGTTA ACTTGGCCCA CAAGTAATGT GGTTATTGAG 
GAGACTCAGC CAGGGAAGAT AAAGATGCTG TTAGAGAAAT TTGTTGAAGA ATGCAGGTTC 
CCTCCAGTGC CGGATGCCAC TTGTTGCTAT CAGAAGTGCC ATGGATATTC CAAAATCCAG 
ATATATATAA CTGATCCAGA CTTTAAGGGT TTTATACGGA TCAGCTGTTG CCAGTATTGT 
AAAATAGAAT TTCACATGAA CTGCTGGAAG AAGTTAAAAA CAACAACCTT CAATGATAAA 
ATTGACAAGG ATTTTCTGCA AGGAATCTGT CTTACTCCTG ATTGTGAAGG AATCATTTCA 
AAGATTATCA TCTTCAGCAG TGGTGGTCAA GTTAAATGTG AATTTGAACA CAAGGTCATT 
AAAGAAAAGG TTCCTCCAAG ACCTATTCTG AAACAGAAAT GTTCTAGCCT AGAGAAGTTG 
AGGCTGAAAG AAGACAAAAA ATTGAAGCGA AAGCTCCAAA AAAAGGAAGC AAAAAAATTA 
GCACAAGAAA GACTGGAAGA GGACTTAAGG GAAAGTAACC CACCAAAAAC TGAAGAGCAG 
AGAGAAACTA TCAACCATGT CCAAAGTTGC CAGTTCCTTG ATGACAGAAT TCTGCAGTGC 
ATAAAGCAGT ACGCTGACAA GATTAAGTCC GGGATACTGA ATACTTCCAA GCTTCTCAAA 
GAATTGCTTT CTTGGAAGGT TTTAACCACA GAAGACTACA CAACGTGTTT TTCTAGCAGA 
AATTTCCTGA ATGAAGCAGT GGACTATGTC ATTCGTCATT TGATCCAAGA AAAGAATAGA 
GTAAAGACAA GGGTATTTCT GCATGTTTTG AGTGAGCTTA AAGAAGTGGA CCCAAAACTA 
GCCACCTGGA TCCAGAAACT GAATAGCTTT GGCTTAGATG CCACAGGACC TTTCTTTTCT 
CGTTATGGGG CTCCTCTTAA GGAGCTTGAT TTTAGCATCA TGACTTTTCT CTGGAATGAG 
AAATACGGTC ATAAGCTAGG CTCTATAGAA GGAAAGCAAC TTGATTATTT CTATGAGCCT 
ACATCACTGA AGGAAGCGCG GTGTTTAATA TGGCTGCTAG AAGAACACAG AGACAAGTTC 
CCAGCACTGC ATAATGCCTT GGACGAGTTC TTTGATATAA TGGACAGCCG CTGCACTGTA 
TTAAGGAAAC AGGACAGTGG TGACGTGCCA TTTAATTCTA CCAAGATAAA AAACAAAGGC 
AAGAAAAAGA AGCCAAAAGA TTCAAAGCCT ATGTTAGTTG GATCTGGAAC ACCTTCAGTC 
ACACCAAACA ATGAGACTGT CACTTCAGGT GAAGACCATA TCAAACGCAG TTTAAGTTCT 
GCGGGCCCCT TTGTAGTGCC TGATCACCTT CGGCAGGATA TAGAAGAATT TGAAGCTCTG 
TATGAACAGC ATAGTAATGA GTATGTTGTC CGTAATAAGA AGCTGTGGGA CATAAACCCA 
AAACAGAAAT GTTCAACTCT ATACGATTAT TTCTCTCAGT TGTTGGAGGA ACATGGTCCC 
TTGGACATGA GTAACAAAAT GTTTTCTGAA GAATATGAGT TCTTCCCAGA AGAAACTCAA 
CAGATACTAG AAAAAGCAGG AGGCTTGAAA GCGTTTCTCC TGGGATGTCC TCGTTTTGTT 
GTGATTGATA ACTGTATCGC ATTGAAGAAA GTTGCATTAC GGCTCAAGAA AAAGAGAAAG 
AAGAAAAACA TTAAAACAAA AGTAGAAGAA ATTTCAAAAA CAGGAGAGTA TTTACGAGTT 
AAACTACCAC TGAATCCAAC TGCTAGGGAA TTTAAACCAG ATGTAAAGTC CAAACCAGTG 
TCTGACTTAC CTTCAGCACC AGCTTCTGAG GACATGAAGC CTCAGCCTGC ACCTGCTAAT 
TCTCCCCAAT CAGCTCGTGA CACTGGGAAG CCAAAACCAG TTTTCGATAA TTCTTCCAGA 
TCAGTTTCTG AGGATGAGAA GCCCAAAGGG ATGTCTTCTG ATTCTCCCAG GCCAGTCTTT 
GAGGATACCG GTCACAAGCC GGTCTCCTCT AATTCTCCCA AACCAGTTCC TGAGGAAGTG 
AAAGCGACCT ATTGGCCTCG ACCCCAGTTG GTCACAGGAT ACTGTACATA TCTTCCCTTT 
CGGGGATTTG ACATTACCCA GACTCCACCA GCCTTCATCA GTGTGTTACC GACTTTGCCC 
CAGTATAGCA TTTACACCCC TCTGGCCAGT GTTCCTACTG AGTATCACCT GCAAAGACCA 
ATACCAGTGG TGCCGTCTTT TGTAGCCAGT GACATAACGG AAGTGCATTT TGAGGCTCAT 
CATTTCAGTG CTGAGAATGC CCCTGGTACC CAGATTGCCT CCGAAACACA GATCCTTGAG 
GAATCTTTGG AAACACCAGT GAAGTCAGAG TGCAGCTATG ATGGTGCTGA TATAGTCCAG 
AGTGAGGCTA GCAGAAACGA TGACTACTGT GGCGATTCTG CCAAATGGGA AGTAAATCCA 
GAAAGCACCA ATGAAGTAAC AAATATTCCA TGTACACAAG TGGTTGCTGT ACAGGTGTCT 
TGGAATATAA CACATCAGGA AGTCAATACT GAGCCATATG GTCCTTTTGA GGCACAACAG 
GGGGGTATTT CACAGGTTGA AAAGGAGTAC CAGGTTTTAC AAGAGCAGCT TAAGGAAGCA 
TGTGAAAATT ATGAACACAG CAATGTCAAG GGCTCAGAAG AAACCAAGGA CTTGGAAGAA 
AAGTTGAAAA GGAATGTTGA AGAAAATAAG ATCTCAAAGA CAGAACTAGA TTGGTTCCTT 
GAAGATTTGA ACAAGGAAAT TAAAAAATGG GAACAGGAGA AAAAAGAAAT CCAAGAAAGA 
CTAAAGGCAC TGAAGAAGAA AATGAAAAAG GTGTTAAATG CCAGCAAAAT GTATACGCAG 
GAAAATGATG GAAAGGAAAA GGCACTTGAA TTACTTCTGG ATCAGTCCCT TGAAATCAGT 
AATACATTTA CAAATGAGAA AATGAAAATA GAAGAGAGTA TAAAGAAAGG GAAAGAGCAT 
TATGAAGAGA GTCTTCAAAG GGCTGTGGAT GCAGAGGTAT CTGTGCTTGA AAACTGGAAG 
GACATGGAAG TGTGTAAGTT ACAGAGCATG GAGTCACAAG CCGAAAGGTT TCTCAAGAAT 
CTGAAGCTAA TGAGCAGTGA TTCGGCAGCG TATTCTGACA TGGACTCAGA CATACATTCA 
TGGGAATCGT TTCTTTCTGA TGTTAGAGAA GAAATTGAGA AAGCAAAGTC TCAGTTTGAA 
GAACAAATTC AGGCAATTAA ATATGGTTCT CAGCTCAGTG AACTTCCTAA AGTGCAGATT 
TCTGAGCTTT CATTTCCTAC CTGTAACACG ATTCATCCTA AATTACTTCC TGAGTCTTCA 
GGCCAGGATG GTCAAGGTCC CTCCACTTCT ACAAGTCATG GGACCGGAGA CCAGACAGCA 
GCTCCCGAGG AGTCAAGTCT GGGCTCTGCA GCTGACAGCC CAGTGGAGTT GCCCTCCTGC 
AGTCAGAAAG CCCCCCAGCT GCCAGAGCAT GAGGGTGCTG GCCAGGCAAC TGGGCCAGAA 
CCAGGCCCAG TGGCTGATCA GAAGTTACCT GCTGCTCCAG AGCGTGCCAC TCATTCAAGC 
CAGTCTCCAA AGAAGCCGTT CAATAGCATT ATTGAGCACC TGTTGGTGGT GTTCCCGTGT 
TACAGCAGCA CGGAGCTTGC TGGTTTTATT AAAAAAGTTA GAAACAAAAA CAAGACCTCT 
CTGTCTGGAT TGAGTATTGA CGAAATTGTG CAAAGAGTGA CAGAACACGT CCTTGATGAA 
GAAAGAAAGA AAAAGCCAAG TCCAGGAAAG GGCTGGAGGC TGTCTGCACC CAGCTCTGCT 
GTTGCTGTGC CCAGGGCTGC CCAGGACCCG CCCTCCATGC CTGTCGGAGC ATCGCCCGAA 
ACCAGGGGGC CGAGGACCGA GGACGTCCCT GTGTCCTGCG CAAATTCCTG TGAAATGTGC 
CGAGAAGTAT TCAAGTCCAA ACACGTGCGT GAGCTGCAGT GTGGGCACAG GCTTCACAAG 
GGGTGCTTCA AGCAGTGGCT GAAGAGCCAG CGCACCTGCC CAGCCTGCCT GGGTCACGAC 
CTCCTGTCAG AGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001103237.2
CDS	1..5958
Translation	MADFPQGNFADYALLEDCPYVDDCIFAPEFMSSDYVRVTQLYCDGVGRQYKDYAQTEGNLEFDICTLWCSQPISVLQDYCDAIKVHIFWPLLFQRQQSSIITRMHSCVRTSGSHACEISLKKLQQLELMEDIVDLAKKVVNDTFFIGGLLRIGYKIENKILAMEEALNWVKYTGDVTILSKLGAVDNCWRMLSIFFTEYKYHITKVVTENCSLLEEFKTQSCAECLEQGELMKMKGNEEFSKERYDIAIIYYTRAIECRPENHLLYGNRALCFLRTGQFRNALGDGKRATILKNNWTKGHYRYCAALSMLGEYDWALQANIKAQKLCKNDPEGIKDLIQQHVKLQKQIEDLQGQTPASNPVKAFCESRAYIPSLSAPAFSTSLNFVETEKNFRKTNHEMANGGNQNPKVVDEALKVDDCDCHPEFVPPSSQPPKYKGKQKSRNNELEKFSPGSQETLPVDLKNILEKQFSKSSRAAHQDFANIMKMLRSLIQDGYTALLEQRCRSAAQAFTELLNGLDPQKIKQLNLAMINYVLVVYGLAISLLGIGQPEELSEAENQFKRMIEHYPNEGLDCLAYCGIGKVYLKKNRFLEALNHFEKARTLICRLPGVLTWPTSNVVIEETQPGKIKMLLEKFVEECRFPPVPDATCCYQKCHGYSKIQIYITDPDFKGFIRISCCQYCKIEFHMNCWKKLKTTTFNDKIDKDFLQGICLTPDCEGIISKIIIFSSGGQVKCEFEHKVIKEKVPPRPILKQKCSSLEKLRLKEDKKLKRKLQKKEAKKLAQERLEEDLRESNPPKTEEQRETINHVQSCQFLDDRILQCIKQYADKIKSGILNTSKLLKELLSWKVLTTEDYTTCFSSRNFLNEAVDYVIRHLIQEKNRVKTRVFLHVLSELKEVDPKLATWIQKLNSFGLDATGPFFSRYGAPLKELDFSIMTFLWNEKYGHKLGSIEGKQLDYFYEPTSLKEARCLIWLLEEHRDKFPALHNALDEFFDIMDSRCTVLRKQDSGDVPFNSTKIKNKGKKKKPKDSKPMLVGSGTPSVTPNNETVTSGEDHIKRSLSSAGPFVVPDHLRQDIEEFEALYEQHSNEYVVRNKKLWDINPKQKCSTLYDYFSQLLEEHGPLDMSNKMFSEEYEFFPEETQQILEKAGGLKAFLLGCPRFVVIDNCIALKKVALRLKKKRKKKNIKTKVEEISKTGEYLRVKLPLNPTAREFKPDVKSKPVSDLPSAPASEDMKPQPAPANSPQSARDTGKPKPVFDNSSRSVSEDEKPKGMSSDSPRPVFEDTGHKPVSSNSPKPVPEEVKATYWPRPQLVTGYCTYLPFRGFDITQTPPAFISVLPTLPQYSIYTPLASVPTEYHLQRPIPVVPSFVASDITEVHFEAHHFSAENAPGTQIASETQILEESLETPVKSECSYDGADIVQSEASRNDDYCGDSAKWEVNPESTNEVTNIPCTQVVAVQVSWNITHQEVNTEPYGPFEAQQGGISQVEKEYQVLQEQLKEACENYEHSNVKGSEETKDLEEKLKRNVEENKISKTELDWFLEDLNKEIKKWEQEKKEIQERLKALKKKMKKVLNASKMYTQENDGKEKALELLLDQSLEISNTFTNEKMKIEESIKKGKEHYEESLQRAVDAEVSVLENWKDMEVCKLQSMESQAERFLKNLKLMSSDSAAYSDMDSDIHSWESFLSDVREEIEKAKSQFEEQIQAIKYGSQLSELPKVQISELSFPTCNTIHPKLLPESSGQDGQGPSTSTSHGTGDQTAAPEESSLGSAADSPVELPSCSQKAPQLPEHEGAGQATGPEPGPVADQKLPAAPERATHSSQSPKKPFNSIIEHLLVVFPCYSSTELAGFIKKVRNKNKTSLSGLSIDEIVQRVTEHVLDEERKKKPSPGKGWRLSAPSSAVAVPRAAQDPPSMPVGASPETRGPRTEDVPVSCANSCEMCREVFKSKHVRELQCGHRLHKGCFKQWLKSQRTCPACLGHDLLSEE

Target ORF information:

RefSeq Version	NM_001109767.3
Organism	Bos taurus(cattle)
Definition	Bos taurus tetratricopeptide repeat domain 3 (TTC3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001109767.3

ORF Insert Sequence:

ATGGCCGATT TTCCCCAGGG AAATTTTGCA GATTATGCCT TGTTAGAAGA TTGCCCTTAT 
GTGGATGATT GTATCTTTGC CCCTGAGTTT ATGTCCAGTG ATTATGTTCG TGTGACTCAG 
CTTTACTGTG ATGGAGTGGG TAGACAGTAT AAAGATTATG CCCAAACTGA GGGAAACTTA 
GAATTTGACA TCTGCACTCT GTGGTGTAGT CAACCTATTT CTGTCCTGCA AGATTACTGT 
GATGCCATTA AAGTCCACAT CTTCTGGCCA CTTCTGTTTC AACGTCAGCA GAGCTCTATA 
ATAACACGGA TGCACTCCTG TGTAAGGACC AGCGGTTCTC ATGCTTGTGA GATAAGTTTG 
AAGAAATTAC AGCAGCTTGA ACTGATGGAA GATATTGTGG ATTTGGCAAA GAAAGTTGTG 
AATGATACAT TCTTTATCGG AGGCTTATTG AGAATTGGCT ATAAAATAGA GAATAAAATC 
TTGGCAATGG AAGAAGCTTT GAATTGGGTG AAATACACAG GCGATGTAAC AATTTTATCT 
AAATTAGGAG CAGTTGATAA TTGTTGGCGT ATGTTAAGTA TTTTCTTTAC TGAATACAAG 
TATCACATAA CTAAAGTTGT AACAGAAAAC TGCAGTTTGC TTGAAGAATT TAAAACTCAA 
AGTTGTGCGG AGTGTCTAGA GCAAGGAGAA CTAATGAAAA TGAAAGGAAA TGAAGAGTTT 
TCCAAAGAAA GATATGATAT AGCTATTATC TATTACACCA GAGCCATTGA ATGTAGGCCT 
GAAAATCACC TGCTTTATGG TAACCGAGCT CTTTGTTTTC TTCGTACTGG ACAATTCAGA 
AATGCACTTG GTGATGGAAA GAGAGCTACC ATTCTGAAGA ACAATTGGAC AAAGGGTCAT 
TATCGTTATT GTGCTGCTCT TTCTATGCTG GGGGAATATG ACTGGGCCCT GCAAGCAAAC 
ATAAAAGCTC AAAAACTCTG TAAAAATGAC CCTGAGGGAA TCAAGGATCT AATTCAGCAG 
CATGTAAAGT TACAAAAACA AATAGAAGAC CTGCAAGGTC AAACACCAGC TAGTAATCCA 
GTCAAAGCCT TCTGTGAAAG CAGGGCCTAC ATACCTAGTT TATCAGCACC TGCATTTAGT 
ACTTCACTTA ACTTTGTGGA AACTGAAAAA AATTTCAGAA AAACTAACCA CGAAATGGCA 
AACGGTGGTA ATCAGAATCC AAAGGTGGTA GATGAAGCTC TGAAAGTAGA TGATTGTGAC 
TGTCATCCTG AATTTGTACC GCCATCAAGT CAGCCTCCAA AATATAAAGG GAAACAAAAA 
TCTCGAAACA ATGAATTGGA GAAGTTCAGC CCTGGTTCAC AAGAAACTTT ACCAGTAGAT 
TTAAAAAACA TCTTGGAGAA ACAGTTTTCT AAATCTTCCA GAGCCGCACA CCAGGATTTT 
GCTAATATAA TGAAAATGCT GAGAAGTTTA ATTCAGGATG GCTACACGGC CTTGCTGGAG 
CAGCGCTGCC GCAGCGCCGC CCAAGCCTTC ACAGAGTTGC TCAACGGTTT AGATCCTCAA 
AAAATAAAGC AATTGAACCT GGCCATGATT AACTATGTGT TAGTAGTCTA CGGACTTGCC 
ATTTCACTCC TTGGAATAGG ACAGCCCGAG GAGTTATCTG AAGCTGAAAA CCAGTTCAAG 
AGGATGATTG AACACTACCC CAATGAGGGG CTTGATTGCT TGGCCTACTG TGGAATTGGA 
AAAGTGTATT TGAAAAAAAA CAGATTTTTA GAAGCTCTTA ATCATTTTGA GAAAGCAAGA 
ACCTTGATCT GCCGTCTTCC TGGAGTGTTA ACTTGGCCCA CAAGTAATGT GGTTATTGAG 
GAGACTCAGC CAGGGAAGAT AAAGATGCTG TTAGAGAAAT TTGTTGAAGA ATGCAGGTTC 
CCTCCAGTGC CGGATGCCAC TTGTTGCTAT CAGAAGTGCC ATGGATATTC CAAAATCCAG 
ATATATATAA CTGATCCAGA CTTTAAGGGT TTTATACGGA TCAGCTGTTG CCAGTATTGT 
AAAATAGAAT TTCACATGAA CTGCTGGAAG AAGTTAAAAA CAACAACCTT CAATGATAAA 
ATTGACAAGG ATTTTCTGCA AGGAATCTGT CTTACTCCTG ATTGTGAAGG AATCATTTCA 
AAGATTATCA TCTTCAGCAG TGGTGGTCAA GTTAAATGTG AATTTGAACA CAAGGTCATT 
AAAGAAAAGG TTCCTCCAAG ACCTATTCTG AAACAGAAAT GTTCTAGCCT AGAGAAGTTG 
AGGCTGAAAG AAGACAAAAA ATTGAAGCGA AAGCTCCAAA AAAAGGAAGC AAAAAAATTA 
GCACAAGAAA GACTGGAAGA GGACTTAAGG GAAAGTAACC CACCAAAAAC TGAAGAGCAG 
AGAGAAACTA TCAACCATGT CCAAAGTTGC CAGTTCCTTG ATGACAGAAT TCTGCAGTGC 
ATAAAGCAGT ACGCTGACAA GATTAAGTCC GGGATACTGA ATACTTCCAA GCTTCTCAAA 
GAATTGCTTT CTTGGAAGGT TTTAACCACA GAAGACTACA CAACGTGTTT TTCTAGCAGA 
AATTTCCTGA ATGAAGCAGT GGACTATGTC ATTCGTCATT TGATCCAAGA AAAGAATAGA 
GTAAAGACAA GGGTATTTCT GCATGTTTTG AGTGAGCTTA AAGAAGTGGA CCCAAAACTA 
GCCACCTGGA TCCAGAAACT GAATAGCTTT GGCTTAGATG CCACAGGACC TTTCTTTTCT 
CGTTATGGGG CTCCTCTTAA GGAGCTTGAT TTTAGCATCA TGACTTTTCT CTGGAATGAG 
AAATACGGTC ATAAGCTAGG CTCTATAGAA GGAAAGCAAC TTGATTATTT CTATGAGCCT 
ACATCACTGA AGGAAGCGCG GTGTTTAATA TGGCTGCTAG AAGAACACAG AGACAAGTTC 
CCAGCACTGC ATAATGCCTT GGACGAGTTC TTTGATATAA TGGACAGCCG CTGCACTGTA 
TTAAGGAAAC AGGACAGTGG TGACGTGCCA TTTAATTCTA CCAAGATAAA AAACAAAGGC 
AAGAAAAAGA AGCCAAAAGA TTCAAAGCCT ATGTTAGTTG GATCTGGAAC ACCTTCAGTC 
ACACCAAACA ATGAGACTGT CACTTCAGGT GAAGACCATA TCAAACGCAG TTTAAGTTCT 
GCGGGCCCCT TTGTAGTGCC TGATCACCTT CGGCAGGATA TAGAAGAATT TGAAGCTCTG 
TATGAACAGC ATAGTAATGA GTATGTTGTC CGTAATAAGA AGCTGTGGGA CATAAACCCA 
AAACAGAAAT GTTCAACTCT ATACGATTAT TTCTCTCAGT TGTTGGAGGA ACATGGTCCC 
TTGGACATGA GTAACAAAAT GTTTTCTGAA GAATATGAGT TCTTCCCAGA AGAAACTCAA 
CAGATACTAG AAAAAGCAGG AGGCTTGAAA GCGTTTCTCC TGGGATGTCC TCGTTTTGTT 
GTGATTGATA ACTGTATCGC ATTGAAGAAA GTTGCATTAC GGCTCAAGAA AAAGAGAAAG 
AAGAAAAACA TTAAAACAAA AGTAGAAGAA ATTTCAAAAA CAGGAGAGTA TTTACGAGTT 
AAACTACCAC TGAATCCAAC TGCTAGGGAA TTTAAACCAG ATGTAAAGTC CAAACCAGTG 
TCTGACTTAC CTTCAGCACC AGCTTCTGAG GACATGAAGC CTCAGCCTGC ACCTGCTAAT 
TCTCCCCAAT CAGCTCGTGA CACTGGGAAG CCAAAACCAG TTTTCGATAA TTCTTCCAGA 
TCAGTTTCTG AGGATGAGAA GCCCAAAGGG ATGTCTTCTG ATTCTCCCAG GCCAGTCTTT 
GAGGATACCG GTCACAAGCC GGTCTCCTCT AATTCTCCCA AACCAGTTCC TGAGGAAGTG 
AAAGCGACCT ATTGGCCTCG ACCCCAGTTG GTCACAGGAT ACTGTACATA TCTTCCCTTT 
CGGGGATTTG ACATTACCCA GACTCCACCA GCCTTCATCA GTGTGTTACC GACTTTGCCC 
CAGTATAGCA TTTACACCCC TCTGGCCAGT GTTCCTACTG AGTATCACCT GCAAAGACCA 
ATACCAGTGG TGCCGTCTTT TGTAGCCAGT GACATAACGG AAGTGCATTT TGAGGCTCAT 
CATTTCAGTG CTGAGAATGC CCCTGGTACC CAGATTGCCT CCGAAACACA GATCCTTGAG 
GAATCTTTGG AAACACCAGT GAAGTCAGAG TGCAGCTATG ATGGTGCTGA TATAGTCCAG 
AGTGAGGCTA GCAGAAACGA TGACTACTGT GGCGATTCTG CCAAATGGGA AGTAAATCCA 
GAAAGCACCA ATGAAGTAAC AAATATTCCA TGTACACAAG TGGTTGCTGT ACAGGTGTCT 
TGGAATATAA CACATCAGGA AGTCAATACT GAGCCATATG GTCCTTTTGA GGCACAACAG 
GGGGGTATTT CACAGGTTGA AAAGGAGTAC CAGGTTTTAC AAGAGCAGCT TAAGGAAGCA 
TGTGAAAATT ATGAACACAG CAATGTCAAG GGCTCAGAAG AAACCAAGGA CTTGGAAGAA 
AAGTTGAAAA GGAATGTTGA AGAAAATAAG ATCTCAAAGA CAGAACTAGA TTGGTTCCTT 
GAAGATTTGA ACAAGGAAAT TAAAAAATGG GAACAGGAGA AAAAAGAAAT CCAAGAAAGA 
CTAAAGGCAC TGAAGAAGAA AATGAAAAAG GTGTTAAATG CCAGCAAAAT GTATACGCAG 
GAAAATGATG GAAAGGAAAA GGCACTTGAA TTACTTCTGG ATCAGTCCCT TGAAATCAGT 
AATACATTTA CAAATGAGAA AATGAAAATA GAAGAGAGTA TAAAGAAAGG GAAAGAGCAT 
TATGAAGAGA GTCTTCAAAG GGCTGTGGAT GCAGAGGTAT CTGTGCTTGA AAACTGGAAG 
GACATGGAAG TGTGTAAGTT ACAGAGCATG GAGTCACAAG CCGAAAGGTT TCTCAAGAAT 
CTGAAGCTAA TGAGCAGTGA TTCGGCAGCG TATTCTGACA TGGACTCAGA CATACATTCA 
TGGGAATCGT TTCTTTCTGA TGTTAGAGAA GAAATTGAGA AAGCAAAGTC TCAGTTTGAA 
GAACAAATTC AGGCAATTAA ATATGGTTCT CAGCTCAGTG AACTTCCTAA AGTGCAGATT 
TCTGAGCTTT CATTTCCTAC CTGTAACACG ATTCATCCTA AATTACTTCC TGAGTCTTCA 
GGCCAGGATG GTCAAGGTCC CTCCACTTCT ACAAGTCATG GGACCGGAGA CCAGACAGCA 
GCTCCCGAGG AGTCAAGTCT GGGCTCTGCA GCTGACAGCC CAGTGGAGTT GCCCTCCTGC 
AGTCAGAAAG CCCCCCAGCT GCCAGAGCAT GAGGGTGCTG GCCAGGCAAC TGGGCCAGAA 
CCAGGCCCAG TGGCTGATCA GAAGTTACCT GCTGCTCCAG AGCGTGCCAC TCATTCAAGC 
CAGTCTCCAA AGAAGCCGTT CAATAGCATT ATTGAGCACC TGTTGGTGGT GTTCCCGTGT 
TACAGCAGCA CGGAGCTTGC TGGTTTTATT AAAAAAGTTA GAAACAAAAA CAAGACCTCT 
CTGTCTGGAT TGAGTATTGA CGAAATTGTG CAAAGAGTGA CAGAACACGT CCTTGATGAA 
GAAAGAAAGA AAAAGCCAAG TCCAGGAAAG GGCTGGAGGC TGTCTGCACC CAGCTCTGCT 
GTTGCTGTGC CCAGGGCTGC CCAGGACCCG CCCTCCATGC CTGTCGGAGC ATCGCCCGAA 
ACCAGGGGGC CGAGGACCGA GGACGTCCCT GTGTCCTGCG CAAATTCCTG TGAAATGTGC 
CGAGAAGTAT TCAAGTCCAA ACACGTGCGT GAGCTGCAGT GTGGGCACAG GCTTCACAAG 
GGGTGCTTCA AGCAGTGGCT GAAGAGCCAG CGCACCTGCC CAGCCTGCCT GGGTCACGAC 
CTCCTGTCAG AGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_005202058.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005202115.1 Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa16127
Clone ID Related Accession (Same CDS sequence)	XM_005202058.3 , XM_005202057.3 , NM_001109767.3 , XM_005202056.3
Accession Version	XM_005202058.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5958bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TTC3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103826.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005202058.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGATT TTCCCCAGGG AAATTTTGCA GATTATGCCT TGTTAGAAGA TTGCCCTTAT 
GTGGATGATT GTATCTTTGC CCCTGAGTTT ATGTCCAGTG ATTATGTTCG TGTGACTCAG 
CTTTACTGTG ATGGAGTGGG TAGACAGTAT AAAGATTATG CCCAAACTGA GGGAAACTTA 
GAATTTGACA TCTGCACTCT GTGGTGTAGT CAACCTATTT CTGTCCTGCA AGATTACTGT 
GATGCCATTA AAGTCCACAT CTTCTGGCCA CTTCTGTTTC AACGTCAGCA GAGCTCTATA 
ATAACACGGA TGCACTCCTG TGTAAGGACC AGCGGTTCTC ATGCTTGTGA GATAAGTTTG 
AAGAAATTAC AGCAGCTTGA ACTGATGGAA GATATTGTGG ATTTGGCAAA GAAAGTTGTG 
AATGATACAT TCTTTATCGG AGGCTTATTG AGAATTGGCT ATAAAATAGA GAATAAAATC 
TTGGCAATGG AAGAAGCTTT GAATTGGGTG AAATACACAG GCGATGTAAC AATTTTATCT 
AAATTAGGAG CAGTTGATAA TTGTTGGCGT ATGTTAAGTA TTTTCTTTAC TGAATACAAG 
TATCACATAA CTAAAGTTGT AACAGAAAAC TGCAGTTTGC TTGAAGAATT TAAAACTCAA 
AGTTGTGCGG AGTGTCTAGA GCAAGGAGAA CTAATGAAAA TGAAAGGAAA TGAAGAGTTT 
TCCAAAGAAA GATATGATAT AGCTATTATC TATTACACCA GAGCCATTGA ATGTAGGCCT 
GAAAATCACC TGCTTTATGG TAACCGAGCT CTTTGTTTTC TTCGTACTGG ACAATTCAGA 
AATGCACTTG GTGATGGAAA GAGAGCTACC ATTCTGAAGA ACAATTGGAC AAAGGGTCAT 
TATCGTTATT GTGCTGCTCT TTCTATGCTG GGGGAATATG ACTGGGCCCT GCAAGCAAAC 
ATAAAAGCTC AAAAACTCTG TAAAAATGAC CCTGAGGGAA TCAAGGATCT AATTCAGCAG 
CATGTAAAGT TACAAAAACA AATAGAAGAC CTGCAAGGTC AAACACCAGC TAGTAATCCA 
GTCAAAGCCT TCTGTGAAAG CAGGGCCTAC ATACCTAGTT TATCAGCACC TGCATTTAGT 
ACTTCACTTA ACTTTGTGGA AACTGAAAAA AATTTCAGAA AAACTAACCA CGAAATGGCA 
AACGGTGGTA ATCAGAATCC AAAGGTGGTA GATGAAGCTC TGAAAGTAGA TGATTGTGAC 
TGTCATCCTG AATTTGTACC GCCATCAAGT CAGCCTCCAA AATATAAAGG GAAACAAAAA 
TCTCGAAACA ATGAATTGGA GAAGTTCAGC CCTGGTTCAC AAGAAACTTT ACCAGTAGAT 
TTAAAAAACA TCTTGGAGAA ACAGTTTTCT AAATCTTCCA GAGCCGCACA CCAGGATTTT 
GCTAATATAA TGAAAATGCT GAGAAGTTTA ATTCAGGATG GCTACACGGC CTTGCTGGAG 
CAGCGCTGCC GCAGCGCCGC CCAAGCCTTC ACAGAGTTGC TCAACGGTTT AGATCCTCAA 
AAAATAAAGC AATTGAACCT GGCCATGATT AACTATGTGT TAGTAGTCTA CGGACTTGCC 
ATTTCACTCC TTGGAATAGG ACAGCCCGAG GAGTTATCTG AAGCTGAAAA CCAGTTCAAG 
AGGATGATTG AACACTACCC CAATGAGGGG CTTGATTGCT TGGCCTACTG TGGAATTGGA 
AAAGTGTATT TGAAAAAAAA CAGATTTTTA GAAGCTCTTA ATCATTTTGA GAAAGCAAGA 
ACCTTGATCT GCCGTCTTCC TGGAGTGTTA ACTTGGCCCA CAAGTAATGT GGTTATTGAG 
GAGACTCAGC CAGGGAAGAT AAAGATGCTG TTAGAGAAAT TTGTTGAAGA ATGCAGGTTC 
CCTCCAGTGC CGGATGCCAC TTGTTGCTAT CAGAAGTGCC ATGGATATTC CAAAATCCAG 
ATATATATAA CTGATCCAGA CTTTAAGGGT TTTATACGGA TCAGCTGTTG CCAGTATTGT 
AAAATAGAAT TTCACATGAA CTGCTGGAAG AAGTTAAAAA CAACAACCTT CAATGATAAA 
ATTGACAAGG ATTTTCTGCA AGGAATCTGT CTTACTCCTG ATTGTGAAGG AATCATTTCA 
AAGATTATCA TCTTCAGCAG TGGTGGTCAA GTTAAATGTG AATTTGAACA CAAGGTCATT 
AAAGAAAAGG TTCCTCCAAG ACCTATTCTG AAACAGAAAT GTTCTAGCCT AGAGAAGTTG 
AGGCTGAAAG AAGACAAAAA ATTGAAGCGA AAGCTCCAAA AAAAGGAAGC AAAAAAATTA 
GCACAAGAAA GACTGGAAGA GGACTTAAGG GAAAGTAACC CACCAAAAAC TGAAGAGCAG 
AGAGAAACTA TCAACCATGT CCAAAGTTGC CAGTTCCTTG ATGACAGAAT TCTGCAGTGC 
ATAAAGCAGT ACGCTGACAA GATTAAGTCC GGGATACTGA ATACTTCCAA GCTTCTCAAA 
GAATTGCTTT CTTGGAAGGT TTTAACCACA GAAGACTACA CAACGTGTTT TTCTAGCAGA 
AATTTCCTGA ATGAAGCAGT GGACTATGTC ATTCGTCATT TGATCCAAGA AAAGAATAGA 
GTAAAGACAA GGGTATTTCT GCATGTTTTG AGTGAGCTTA AAGAAGTGGA CCCAAAACTA 
GCCACCTGGA TCCAGAAACT GAATAGCTTT GGCTTAGATG CCACAGGACC TTTCTTTTCT 
CGTTATGGGG CTCCTCTTAA GGAGCTTGAT TTTAGCATCA TGACTTTTCT CTGGAATGAG 
AAATACGGTC ATAAGCTAGG CTCTATAGAA GGAAAGCAAC TTGATTATTT CTATGAGCCT 
ACATCACTGA AGGAAGCGCG GTGTTTAATA TGGCTGCTAG AAGAACACAG AGACAAGTTC 
CCAGCACTGC ATAATGCCTT GGACGAGTTC TTTGATATAA TGGACAGCCG CTGCACTGTA 
TTAAGGAAAC AGGACAGTGG TGACGTGCCA TTTAATTCTA CCAAGATAAA AAACAAAGGC 
AAGAAAAAGA AGCCAAAAGA TTCAAAGCCT ATGTTAGTTG GATCTGGAAC ACCTTCAGTC 
ACACCAAACA ATGAGACTGT CACTTCAGGT GAAGACCATA TCAAACGCAG TTTAAGTTCT 
GCGGGCCCCT TTGTAGTGCC TGATCACCTT CGGCAGGATA TAGAAGAATT TGAAGCTCTG 
TATGAACAGC ATAGTAATGA GTATGTTGTC CGTAATAAGA AGCTGTGGGA CATAAACCCA 
AAACAGAAAT GTTCAACTCT ATACGATTAT TTCTCTCAGT TGTTGGAGGA ACATGGTCCC 
TTGGACATGA GTAACAAAAT GTTTTCTGAA GAATATGAGT TCTTCCCAGA AGAAACTCAA 
CAGATACTAG AAAAAGCAGG AGGCTTGAAA GCGTTTCTCC TGGGATGTCC TCGTTTTGTT 
GTGATTGATA ACTGTATCGC ATTGAAGAAA GTTGCATTAC GGCTCAAGAA AAAGAGAAAG 
AAGAAAAACA TTAAAACAAA AGTAGAAGAA ATTTCAAAAA CAGGAGAGTA TTTACGAGTT 
AAACTACCAC TGAATCCAAC TGCTAGGGAA TTTAAACCAG ATGTAAAGTC CAAACCAGTG 
TCTGACTTAC CTTCAGCACC AGCTTCTGAG GACATGAAGC CTCAGCCTGC ACCTGCTAAT 
TCTCCCCAAT CAGCTCGTGA CACTGGGAAG CCAAAACCAG TTTTCGATAA TTCTTCCAGA 
TCAGTTTCTG AGGATGAGAA GCCCAAAGGG ATGTCTTCTG ATTCTCCCAG GCCAGTCTTT 
GAGGATACCG GTCACAAGCC GGTCTCCTCT AATTCTCCCA AACCAGTTCC TGAGGAAGTG 
AAAGCGACCT ATTGGCCTCG ACCCCAGTTG GTCACAGGAT ACTGTACATA TCTTCCCTTT 
CGGGGATTTG ACATTACCCA GACTCCACCA GCCTTCATCA GTGTGTTACC GACTTTGCCC 
CAGTATAGCA TTTACACCCC TCTGGCCAGT GTTCCTACTG AGTATCACCT GCAAAGACCA 
ATACCAGTGG TGCCGTCTTT TGTAGCCAGT GACATAACGG AAGTGCATTT TGAGGCTCAT 
CATTTCAGTG CTGAGAATGC CCCTGGTACC CAGATTGCCT CCGAAACACA GATCCTTGAG 
GAATCTTTGG AAACACCAGT GAAGTCAGAG TGCAGCTATG ATGGTGCTGA TATAGTCCAG 
AGTGAGGCTA GCAGAAACGA TGACTACTGT GGCGATTCTG CCAAATGGGA AGTAAATCCA 
GAAAGCACCA ATGAAGTAAC AAATATTCCA TGTACACAAG TGGTTGCTGT ACAGGTGTCT 
TGGAATATAA CACATCAGGA AGTCAATACT GAGCCATATG GTCCTTTTGA GGCACAACAG 
GGGGGTATTT CACAGGTTGA AAAGGAGTAC CAGGTTTTAC AAGAGCAGCT TAAGGAAGCA 
TGTGAAAATT ATGAACACAG CAATGTCAAG GGCTCAGAAG AAACCAAGGA CTTGGAAGAA 
AAGTTGAAAA GGAATGTTGA AGAAAATAAG ATCTCAAAGA CAGAACTAGA TTGGTTCCTT 
GAAGATTTGA ACAAGGAAAT TAAAAAATGG GAACAGGAGA AAAAAGAAAT CCAAGAAAGA 
CTAAAGGCAC TGAAGAAGAA AATGAAAAAG GTGTTAAATG CCAGCAAAAT GTATACGCAG 
GAAAATGATG GAAAGGAAAA GGCACTTGAA TTACTTCTGG ATCAGTCCCT TGAAATCAGT 
AATACATTTA CAAATGAGAA AATGAAAATA GAAGAGAGTA TAAAGAAAGG GAAAGAGCAT 
TATGAAGAGA GTCTTCAAAG GGCTGTGGAT GCAGAGGTAT CTGTGCTTGA AAACTGGAAG 
GACATGGAAG TGTGTAAGTT ACAGAGCATG GAGTCACAAG CCGAAAGGTT TCTCAAGAAT 
CTGAAGCTAA TGAGCAGTGA TTCGGCAGCG TATTCTGACA TGGACTCAGA CATACATTCA 
TGGGAATCGT TTCTTTCTGA TGTTAGAGAA GAAATTGAGA AAGCAAAGTC TCAGTTTGAA 
GAACAAATTC AGGCAATTAA ATATGGTTCT CAGCTCAGTG AACTTCCTAA AGTGCAGATT 
TCTGAGCTTT CATTTCCTAC CTGTAACACG ATTCATCCTA AATTACTTCC TGAGTCTTCA 
GGCCAGGATG GTCAAGGTCC CTCCACTTCT ACAAGTCATG GGACCGGAGA CCAGACAGCA 
GCTCCCGAGG AGTCAAGTCT GGGCTCTGCA GCTGACAGCC CAGTGGAGTT GCCCTCCTGC 
AGTCAGAAAG CCCCCCAGCT GCCAGAGCAT GAGGGTGCTG GCCAGGCAAC TGGGCCAGAA 
CCAGGCCCAG TGGCTGATCA GAAGTTACCT GCTGCTCCAG AGCGTGCCAC TCATTCAAGC 
CAGTCTCCAA AGAAGCCGTT CAATAGCATT ATTGAGCACC TGTTGGTGGT GTTCCCGTGT 
TACAGCAGCA CGGAGCTTGC TGGTTTTATT AAAAAAGTTA GAAACAAAAA CAAGACCTCT 
CTGTCTGGAT TGAGTATTGA CGAAATTGTG CAAAGAGTGA CAGAACACGT CCTTGATGAA 
GAAAGAAAGA AAAAGCCAAG TCCAGGAAAG GGCTGGAGGC TGTCTGCACC CAGCTCTGCT 
GTTGCTGTGC CCAGGGCTGC CCAGGACCCG CCCTCCATGC CTGTCGGAGC ATCGCCCGAA 
ACCAGGGGGC CGAGGACCGA GGACGTCCCT GTGTCCTGCG CAAATTCCTG TGAAATGTGC 
CGAGAAGTAT TCAAGTCCAA ACACGTGCGT GAGCTGCAGT GTGGGCACAG GCTTCACAAG 
GGGTGCTTCA AGCAGTGGCT GAAGAGCCAG CGCACCTGCC CAGCCTGCCT GGGTCACGAC 
CTCCTGTCAG AGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005202115.1
CDS	78..6035
Translation	MADFPQGNFADYALLEDCPYVDDCIFAPEFMSSDYVRVTQLYCDGVGRQYKDYAQTEGNLEFDICTLWCSQPISVLQDYCDAIKVHIFWPLLFQRQQSSIITRMHSCVRTSGSHACEISLKKLQQLELMEDIVDLAKKVVNDTFFIGGLLRIGYKIENKILAMEEALNWVKYTGDVTILSKLGAVDNCWRMLSIFFTEYKYHITKVVTENCSLLEEFKTQSCAECLEQGELMKMKGNEEFSKERYDIAIIYYTRAIECRPENHLLYGNRALCFLRTGQFRNALGDGKRATILKNNWTKGHYRYCAALSMLGEYDWALQANIKAQKLCKNDPEGIKDLIQQHVKLQKQIEDLQGQTPASNPVKAFCESRAYIPSLSAPAFSTSLNFVETEKNFRKTNHEMANGGNQNPKVVDEALKVDDCDCHPEFVPPSSQPPKYKGKQKSRNNELEKFSPGSQETLPVDLKNILEKQFSKSSRAAHQDFANIMKMLRSLIQDGYTALLEQRCRSAAQAFTELLNGLDPQKIKQLNLAMINYVLVVYGLAISLLGIGQPEELSEAENQFKRMIEHYPNEGLDCLAYCGIGKVYLKKNRFLEALNHFEKARTLICRLPGVLTWPTSNVVIEETQPGKIKMLLEKFVEECRFPPVPDATCCYQKCHGYSKIQIYITDPDFKGFIRISCCQYCKIEFHMNCWKKLKTTTFNDKIDKDFLQGICLTPDCEGIISKIIIFSSGGQVKCEFEHKVIKEKVPPRPILKQKCSSLEKLRLKEDKKLKRKLQKKEAKKLAQERLEEDLRESNPPKTEEQRETINHVQSCQFLDDRILQCIKQYADKIKSGILNTSKLLKELLSWKVLTTEDYTTCFSSRNFLNEAVDYVIRHLIQEKNRVKTRVFLHVLSELKEVDPKLATWIQKLNSFGLDATGPFFSRYGAPLKELDFSIMTFLWNEKYGHKLGSIEGKQLDYFYEPTSLKEARCLIWLLEEHRDKFPALHNALDEFFDIMDSRCTVLRKQDSGDVPFNSTKIKNKGKKKKPKDSKPMLVGSGTPSVTPNNETVTSGEDHIKRSLSSAGPFVVPDHLRQDIEEFEALYEQHSNEYVVRNKKLWDINPKQKCSTLYDYFSQLLEEHGPLDMSNKMFSEEYEFFPEETQQILEKAGGLKAFLLGCPRFVVIDNCIALKKVALRLKKKRKKKNIKTKVEEISKTGEYLRVKLPLNPTAREFKPDVKSKPVSDLPSAPASEDMKPQPAPANSPQSARDTGKPKPVFDNSSRSVSEDEKPKGMSSDSPRPVFEDTGHKPVSSNSPKPVPEEVKATYWPRPQLVTGYCTYLPFRGFDITQTPPAFISVLPTLPQYSIYTPLASVPTEYHLQRPIPVVPSFVASDITEVHFEAHHFSAENAPGTQIASETQILEESLETPVKSECSYDGADIVQSEASRNDDYCGDSAKWEVNPESTNEVTNIPCTQVVAVQVSWNITHQEVNTEPYGPFEAQQGGISQVEKEYQVLQEQLKEACENYEHSNVKGSEETKDLEEKLKRNVEENKISKTELDWFLEDLNKEIKKWEQEKKEIQERLKALKKKMKKVLNASKMYTQENDGKEKALELLLDQSLEISNTFTNEKMKIEESIKKGKEHYEESLQRAVDAEVSVLENWKDMEVCKLQSMESQAERFLKNLKLMSSDSAAYSDMDSDIHSWESFLSDVREEIEKAKSQFEEQIQAIKYGSQLSELPKVQISELSFPTCNTIHPKLLPESSGQDGQGPSTSTSHGTGDQTAAPEESSLGSAADSPVELPSCSQKAPQLPEHEGAGQATGPEPGPVADQKLPAAPERATHSSQSPKKPFNSIIEHLLVVFPCYSSTELAGFIKKVRNKNKTSLSGLSIDEIVQRVTEHVLDEERKKKPSPGKGWRLSAPSSAVAVPRAAQDPPSMPVGASPETRGPRTEDVPVSCANSCEMCREVFKSKHVRELQCGHRLHKGCFKQWLKSQRTCPACLGHDLLSEE

Target ORF information:

RefSeq Version	XM_005202058.3
Organism	Bos taurus(cattle)
Definition	Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005202058.3

ORF Insert Sequence:

ATGGCCGATT TTCCCCAGGG AAATTTTGCA GATTATGCCT TGTTAGAAGA TTGCCCTTAT 
GTGGATGATT GTATCTTTGC CCCTGAGTTT ATGTCCAGTG ATTATGTTCG TGTGACTCAG 
CTTTACTGTG ATGGAGTGGG TAGACAGTAT AAAGATTATG CCCAAACTGA GGGAAACTTA 
GAATTTGACA TCTGCACTCT GTGGTGTAGT CAACCTATTT CTGTCCTGCA AGATTACTGT 
GATGCCATTA AAGTCCACAT CTTCTGGCCA CTTCTGTTTC AACGTCAGCA GAGCTCTATA 
ATAACACGGA TGCACTCCTG TGTAAGGACC AGCGGTTCTC ATGCTTGTGA GATAAGTTTG 
AAGAAATTAC AGCAGCTTGA ACTGATGGAA GATATTGTGG ATTTGGCAAA GAAAGTTGTG 
AATGATACAT TCTTTATCGG AGGCTTATTG AGAATTGGCT ATAAAATAGA GAATAAAATC 
TTGGCAATGG AAGAAGCTTT GAATTGGGTG AAATACACAG GCGATGTAAC AATTTTATCT 
AAATTAGGAG CAGTTGATAA TTGTTGGCGT ATGTTAAGTA TTTTCTTTAC TGAATACAAG 
TATCACATAA CTAAAGTTGT AACAGAAAAC TGCAGTTTGC TTGAAGAATT TAAAACTCAA 
AGTTGTGCGG AGTGTCTAGA GCAAGGAGAA CTAATGAAAA TGAAAGGAAA TGAAGAGTTT 
TCCAAAGAAA GATATGATAT AGCTATTATC TATTACACCA GAGCCATTGA ATGTAGGCCT 
GAAAATCACC TGCTTTATGG TAACCGAGCT CTTTGTTTTC TTCGTACTGG ACAATTCAGA 
AATGCACTTG GTGATGGAAA GAGAGCTACC ATTCTGAAGA ACAATTGGAC AAAGGGTCAT 
TATCGTTATT GTGCTGCTCT TTCTATGCTG GGGGAATATG ACTGGGCCCT GCAAGCAAAC 
ATAAAAGCTC AAAAACTCTG TAAAAATGAC CCTGAGGGAA TCAAGGATCT AATTCAGCAG 
CATGTAAAGT TACAAAAACA AATAGAAGAC CTGCAAGGTC AAACACCAGC TAGTAATCCA 
GTCAAAGCCT TCTGTGAAAG CAGGGCCTAC ATACCTAGTT TATCAGCACC TGCATTTAGT 
ACTTCACTTA ACTTTGTGGA AACTGAAAAA AATTTCAGAA AAACTAACCA CGAAATGGCA 
AACGGTGGTA ATCAGAATCC AAAGGTGGTA GATGAAGCTC TGAAAGTAGA TGATTGTGAC 
TGTCATCCTG AATTTGTACC GCCATCAAGT CAGCCTCCAA AATATAAAGG GAAACAAAAA 
TCTCGAAACA ATGAATTGGA GAAGTTCAGC CCTGGTTCAC AAGAAACTTT ACCAGTAGAT 
TTAAAAAACA TCTTGGAGAA ACAGTTTTCT AAATCTTCCA GAGCCGCACA CCAGGATTTT 
GCTAATATAA TGAAAATGCT GAGAAGTTTA ATTCAGGATG GCTACACGGC CTTGCTGGAG 
CAGCGCTGCC GCAGCGCCGC CCAAGCCTTC ACAGAGTTGC TCAACGGTTT AGATCCTCAA 
AAAATAAAGC AATTGAACCT GGCCATGATT AACTATGTGT TAGTAGTCTA CGGACTTGCC 
ATTTCACTCC TTGGAATAGG ACAGCCCGAG GAGTTATCTG AAGCTGAAAA CCAGTTCAAG 
AGGATGATTG AACACTACCC CAATGAGGGG CTTGATTGCT TGGCCTACTG TGGAATTGGA 
AAAGTGTATT TGAAAAAAAA CAGATTTTTA GAAGCTCTTA ATCATTTTGA GAAAGCAAGA 
ACCTTGATCT GCCGTCTTCC TGGAGTGTTA ACTTGGCCCA CAAGTAATGT GGTTATTGAG 
GAGACTCAGC CAGGGAAGAT AAAGATGCTG TTAGAGAAAT TTGTTGAAGA ATGCAGGTTC 
CCTCCAGTGC CGGATGCCAC TTGTTGCTAT CAGAAGTGCC ATGGATATTC CAAAATCCAG 
ATATATATAA CTGATCCAGA CTTTAAGGGT TTTATACGGA TCAGCTGTTG CCAGTATTGT 
AAAATAGAAT TTCACATGAA CTGCTGGAAG AAGTTAAAAA CAACAACCTT CAATGATAAA 
ATTGACAAGG ATTTTCTGCA AGGAATCTGT CTTACTCCTG ATTGTGAAGG AATCATTTCA 
AAGATTATCA TCTTCAGCAG TGGTGGTCAA GTTAAATGTG AATTTGAACA CAAGGTCATT 
AAAGAAAAGG TTCCTCCAAG ACCTATTCTG AAACAGAAAT GTTCTAGCCT AGAGAAGTTG 
AGGCTGAAAG AAGACAAAAA ATTGAAGCGA AAGCTCCAAA AAAAGGAAGC AAAAAAATTA 
GCACAAGAAA GACTGGAAGA GGACTTAAGG GAAAGTAACC CACCAAAAAC TGAAGAGCAG 
AGAGAAACTA TCAACCATGT CCAAAGTTGC CAGTTCCTTG ATGACAGAAT TCTGCAGTGC 
ATAAAGCAGT ACGCTGACAA GATTAAGTCC GGGATACTGA ATACTTCCAA GCTTCTCAAA 
GAATTGCTTT CTTGGAAGGT TTTAACCACA GAAGACTACA CAACGTGTTT TTCTAGCAGA 
AATTTCCTGA ATGAAGCAGT GGACTATGTC ATTCGTCATT TGATCCAAGA AAAGAATAGA 
GTAAAGACAA GGGTATTTCT GCATGTTTTG AGTGAGCTTA AAGAAGTGGA CCCAAAACTA 
GCCACCTGGA TCCAGAAACT GAATAGCTTT GGCTTAGATG CCACAGGACC TTTCTTTTCT 
CGTTATGGGG CTCCTCTTAA GGAGCTTGAT TTTAGCATCA TGACTTTTCT CTGGAATGAG 
AAATACGGTC ATAAGCTAGG CTCTATAGAA GGAAAGCAAC TTGATTATTT CTATGAGCCT 
ACATCACTGA AGGAAGCGCG GTGTTTAATA TGGCTGCTAG AAGAACACAG AGACAAGTTC 
CCAGCACTGC ATAATGCCTT GGACGAGTTC TTTGATATAA TGGACAGCCG CTGCACTGTA 
TTAAGGAAAC AGGACAGTGG TGACGTGCCA TTTAATTCTA CCAAGATAAA AAACAAAGGC 
AAGAAAAAGA AGCCAAAAGA TTCAAAGCCT ATGTTAGTTG GATCTGGAAC ACCTTCAGTC 
ACACCAAACA ATGAGACTGT CACTTCAGGT GAAGACCATA TCAAACGCAG TTTAAGTTCT 
GCGGGCCCCT TTGTAGTGCC TGATCACCTT CGGCAGGATA TAGAAGAATT TGAAGCTCTG 
TATGAACAGC ATAGTAATGA GTATGTTGTC CGTAATAAGA AGCTGTGGGA CATAAACCCA 
AAACAGAAAT GTTCAACTCT ATACGATTAT TTCTCTCAGT TGTTGGAGGA ACATGGTCCC 
TTGGACATGA GTAACAAAAT GTTTTCTGAA GAATATGAGT TCTTCCCAGA AGAAACTCAA 
CAGATACTAG AAAAAGCAGG AGGCTTGAAA GCGTTTCTCC TGGGATGTCC TCGTTTTGTT 
GTGATTGATA ACTGTATCGC ATTGAAGAAA GTTGCATTAC GGCTCAAGAA AAAGAGAAAG 
AAGAAAAACA TTAAAACAAA AGTAGAAGAA ATTTCAAAAA CAGGAGAGTA TTTACGAGTT 
AAACTACCAC TGAATCCAAC TGCTAGGGAA TTTAAACCAG ATGTAAAGTC CAAACCAGTG 
TCTGACTTAC CTTCAGCACC AGCTTCTGAG GACATGAAGC CTCAGCCTGC ACCTGCTAAT 
TCTCCCCAAT CAGCTCGTGA CACTGGGAAG CCAAAACCAG TTTTCGATAA TTCTTCCAGA 
TCAGTTTCTG AGGATGAGAA GCCCAAAGGG ATGTCTTCTG ATTCTCCCAG GCCAGTCTTT 
GAGGATACCG GTCACAAGCC GGTCTCCTCT AATTCTCCCA AACCAGTTCC TGAGGAAGTG 
AAAGCGACCT ATTGGCCTCG ACCCCAGTTG GTCACAGGAT ACTGTACATA TCTTCCCTTT 
CGGGGATTTG ACATTACCCA GACTCCACCA GCCTTCATCA GTGTGTTACC GACTTTGCCC 
CAGTATAGCA TTTACACCCC TCTGGCCAGT GTTCCTACTG AGTATCACCT GCAAAGACCA 
ATACCAGTGG TGCCGTCTTT TGTAGCCAGT GACATAACGG AAGTGCATTT TGAGGCTCAT 
CATTTCAGTG CTGAGAATGC CCCTGGTACC CAGATTGCCT CCGAAACACA GATCCTTGAG 
GAATCTTTGG AAACACCAGT GAAGTCAGAG TGCAGCTATG ATGGTGCTGA TATAGTCCAG 
AGTGAGGCTA GCAGAAACGA TGACTACTGT GGCGATTCTG CCAAATGGGA AGTAAATCCA 
GAAAGCACCA ATGAAGTAAC AAATATTCCA TGTACACAAG TGGTTGCTGT ACAGGTGTCT 
TGGAATATAA CACATCAGGA AGTCAATACT GAGCCATATG GTCCTTTTGA GGCACAACAG 
GGGGGTATTT CACAGGTTGA AAAGGAGTAC CAGGTTTTAC AAGAGCAGCT TAAGGAAGCA 
TGTGAAAATT ATGAACACAG CAATGTCAAG GGCTCAGAAG AAACCAAGGA CTTGGAAGAA 
AAGTTGAAAA GGAATGTTGA AGAAAATAAG ATCTCAAAGA CAGAACTAGA TTGGTTCCTT 
GAAGATTTGA ACAAGGAAAT TAAAAAATGG GAACAGGAGA AAAAAGAAAT CCAAGAAAGA 
CTAAAGGCAC TGAAGAAGAA AATGAAAAAG GTGTTAAATG CCAGCAAAAT GTATACGCAG 
GAAAATGATG GAAAGGAAAA GGCACTTGAA TTACTTCTGG ATCAGTCCCT TGAAATCAGT 
AATACATTTA CAAATGAGAA AATGAAAATA GAAGAGAGTA TAAAGAAAGG GAAAGAGCAT 
TATGAAGAGA GTCTTCAAAG GGCTGTGGAT GCAGAGGTAT CTGTGCTTGA AAACTGGAAG 
GACATGGAAG TGTGTAAGTT ACAGAGCATG GAGTCACAAG CCGAAAGGTT TCTCAAGAAT 
CTGAAGCTAA TGAGCAGTGA TTCGGCAGCG TATTCTGACA TGGACTCAGA CATACATTCA 
TGGGAATCGT TTCTTTCTGA TGTTAGAGAA GAAATTGAGA AAGCAAAGTC TCAGTTTGAA 
GAACAAATTC AGGCAATTAA ATATGGTTCT CAGCTCAGTG AACTTCCTAA AGTGCAGATT 
TCTGAGCTTT CATTTCCTAC CTGTAACACG ATTCATCCTA AATTACTTCC TGAGTCTTCA 
GGCCAGGATG GTCAAGGTCC CTCCACTTCT ACAAGTCATG GGACCGGAGA CCAGACAGCA 
GCTCCCGAGG AGTCAAGTCT GGGCTCTGCA GCTGACAGCC CAGTGGAGTT GCCCTCCTGC 
AGTCAGAAAG CCCCCCAGCT GCCAGAGCAT GAGGGTGCTG GCCAGGCAAC TGGGCCAGAA 
CCAGGCCCAG TGGCTGATCA GAAGTTACCT GCTGCTCCAG AGCGTGCCAC TCATTCAAGC 
CAGTCTCCAA AGAAGCCGTT CAATAGCATT ATTGAGCACC TGTTGGTGGT GTTCCCGTGT 
TACAGCAGCA CGGAGCTTGC TGGTTTTATT AAAAAAGTTA GAAACAAAAA CAAGACCTCT 
CTGTCTGGAT TGAGTATTGA CGAAATTGTG CAAAGAGTGA CAGAACACGT CCTTGATGAA 
GAAAGAAAGA AAAAGCCAAG TCCAGGAAAG GGCTGGAGGC TGTCTGCACC CAGCTCTGCT 
GTTGCTGTGC CCAGGGCTGC CCAGGACCCG CCCTCCATGC CTGTCGGAGC ATCGCCCGAA 
ACCAGGGGGC CGAGGACCGA GGACGTCCCT GTGTCCTGCG CAAATTCCTG TGAAATGTGC 
CGAGAAGTAT TCAAGTCCAA ACACGTGCGT GAGCTGCAGT GTGGGCACAG GCTTCACAAG 
GGGTGCTTCA AGCAGTGGCT GAAGAGCCAG CGCACCTGCC CAGCCTGCCT GGGTCACGAC 
CTCCTGTCAG AGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_005202057.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005202114.1 Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X2, mRNA.

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CloneID	OBa16127
Clone ID Related Accession (Same CDS sequence)	XM_005202058.3 , XM_005202057.3 , NM_001109767.3 , XM_005202056.3
Accession Version	XM_005202057.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5958bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TTC3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103826.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005202057.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGATT TTCCCCAGGG AAATTTTGCA GATTATGCCT TGTTAGAAGA TTGCCCTTAT 
GTGGATGATT GTATCTTTGC CCCTGAGTTT ATGTCCAGTG ATTATGTTCG TGTGACTCAG 
CTTTACTGTG ATGGAGTGGG TAGACAGTAT AAAGATTATG CCCAAACTGA GGGAAACTTA 
GAATTTGACA TCTGCACTCT GTGGTGTAGT CAACCTATTT CTGTCCTGCA AGATTACTGT 
GATGCCATTA AAGTCCACAT CTTCTGGCCA CTTCTGTTTC AACGTCAGCA GAGCTCTATA 
ATAACACGGA TGCACTCCTG TGTAAGGACC AGCGGTTCTC ATGCTTGTGA GATAAGTTTG 
AAGAAATTAC AGCAGCTTGA ACTGATGGAA GATATTGTGG ATTTGGCAAA GAAAGTTGTG 
AATGATACAT TCTTTATCGG AGGCTTATTG AGAATTGGCT ATAAAATAGA GAATAAAATC 
TTGGCAATGG AAGAAGCTTT GAATTGGGTG AAATACACAG GCGATGTAAC AATTTTATCT 
AAATTAGGAG CAGTTGATAA TTGTTGGCGT ATGTTAAGTA TTTTCTTTAC TGAATACAAG 
TATCACATAA CTAAAGTTGT AACAGAAAAC TGCAGTTTGC TTGAAGAATT TAAAACTCAA 
AGTTGTGCGG AGTGTCTAGA GCAAGGAGAA CTAATGAAAA TGAAAGGAAA TGAAGAGTTT 
TCCAAAGAAA GATATGATAT AGCTATTATC TATTACACCA GAGCCATTGA ATGTAGGCCT 
GAAAATCACC TGCTTTATGG TAACCGAGCT CTTTGTTTTC TTCGTACTGG ACAATTCAGA 
AATGCACTTG GTGATGGAAA GAGAGCTACC ATTCTGAAGA ACAATTGGAC AAAGGGTCAT 
TATCGTTATT GTGCTGCTCT TTCTATGCTG GGGGAATATG ACTGGGCCCT GCAAGCAAAC 
ATAAAAGCTC AAAAACTCTG TAAAAATGAC CCTGAGGGAA TCAAGGATCT AATTCAGCAG 
CATGTAAAGT TACAAAAACA AATAGAAGAC CTGCAAGGTC AAACACCAGC TAGTAATCCA 
GTCAAAGCCT TCTGTGAAAG CAGGGCCTAC ATACCTAGTT TATCAGCACC TGCATTTAGT 
ACTTCACTTA ACTTTGTGGA AACTGAAAAA AATTTCAGAA AAACTAACCA CGAAATGGCA 
AACGGTGGTA ATCAGAATCC AAAGGTGGTA GATGAAGCTC TGAAAGTAGA TGATTGTGAC 
TGTCATCCTG AATTTGTACC GCCATCAAGT CAGCCTCCAA AATATAAAGG GAAACAAAAA 
TCTCGAAACA ATGAATTGGA GAAGTTCAGC CCTGGTTCAC AAGAAACTTT ACCAGTAGAT 
TTAAAAAACA TCTTGGAGAA ACAGTTTTCT AAATCTTCCA GAGCCGCACA CCAGGATTTT 
GCTAATATAA TGAAAATGCT GAGAAGTTTA ATTCAGGATG GCTACACGGC CTTGCTGGAG 
CAGCGCTGCC GCAGCGCCGC CCAAGCCTTC ACAGAGTTGC TCAACGGTTT AGATCCTCAA 
AAAATAAAGC AATTGAACCT GGCCATGATT AACTATGTGT TAGTAGTCTA CGGACTTGCC 
ATTTCACTCC TTGGAATAGG ACAGCCCGAG GAGTTATCTG AAGCTGAAAA CCAGTTCAAG 
AGGATGATTG AACACTACCC CAATGAGGGG CTTGATTGCT TGGCCTACTG TGGAATTGGA 
AAAGTGTATT TGAAAAAAAA CAGATTTTTA GAAGCTCTTA ATCATTTTGA GAAAGCAAGA 
ACCTTGATCT GCCGTCTTCC TGGAGTGTTA ACTTGGCCCA CAAGTAATGT GGTTATTGAG 
GAGACTCAGC CAGGGAAGAT AAAGATGCTG TTAGAGAAAT TTGTTGAAGA ATGCAGGTTC 
CCTCCAGTGC CGGATGCCAC TTGTTGCTAT CAGAAGTGCC ATGGATATTC CAAAATCCAG 
ATATATATAA CTGATCCAGA CTTTAAGGGT TTTATACGGA TCAGCTGTTG CCAGTATTGT 
AAAATAGAAT TTCACATGAA CTGCTGGAAG AAGTTAAAAA CAACAACCTT CAATGATAAA 
ATTGACAAGG ATTTTCTGCA AGGAATCTGT CTTACTCCTG ATTGTGAAGG AATCATTTCA 
AAGATTATCA TCTTCAGCAG TGGTGGTCAA GTTAAATGTG AATTTGAACA CAAGGTCATT 
AAAGAAAAGG TTCCTCCAAG ACCTATTCTG AAACAGAAAT GTTCTAGCCT AGAGAAGTTG 
AGGCTGAAAG AAGACAAAAA ATTGAAGCGA AAGCTCCAAA AAAAGGAAGC AAAAAAATTA 
GCACAAGAAA GACTGGAAGA GGACTTAAGG GAAAGTAACC CACCAAAAAC TGAAGAGCAG 
AGAGAAACTA TCAACCATGT CCAAAGTTGC CAGTTCCTTG ATGACAGAAT TCTGCAGTGC 
ATAAAGCAGT ACGCTGACAA GATTAAGTCC GGGATACTGA ATACTTCCAA GCTTCTCAAA 
GAATTGCTTT CTTGGAAGGT TTTAACCACA GAAGACTACA CAACGTGTTT TTCTAGCAGA 
AATTTCCTGA ATGAAGCAGT GGACTATGTC ATTCGTCATT TGATCCAAGA AAAGAATAGA 
GTAAAGACAA GGGTATTTCT GCATGTTTTG AGTGAGCTTA AAGAAGTGGA CCCAAAACTA 
GCCACCTGGA TCCAGAAACT GAATAGCTTT GGCTTAGATG CCACAGGACC TTTCTTTTCT 
CGTTATGGGG CTCCTCTTAA GGAGCTTGAT TTTAGCATCA TGACTTTTCT CTGGAATGAG 
AAATACGGTC ATAAGCTAGG CTCTATAGAA GGAAAGCAAC TTGATTATTT CTATGAGCCT 
ACATCACTGA AGGAAGCGCG GTGTTTAATA TGGCTGCTAG AAGAACACAG AGACAAGTTC 
CCAGCACTGC ATAATGCCTT GGACGAGTTC TTTGATATAA TGGACAGCCG CTGCACTGTA 
TTAAGGAAAC AGGACAGTGG TGACGTGCCA TTTAATTCTA CCAAGATAAA AAACAAAGGC 
AAGAAAAAGA AGCCAAAAGA TTCAAAGCCT ATGTTAGTTG GATCTGGAAC ACCTTCAGTC 
ACACCAAACA ATGAGACTGT CACTTCAGGT GAAGACCATA TCAAACGCAG TTTAAGTTCT 
GCGGGCCCCT TTGTAGTGCC TGATCACCTT CGGCAGGATA TAGAAGAATT TGAAGCTCTG 
TATGAACAGC ATAGTAATGA GTATGTTGTC CGTAATAAGA AGCTGTGGGA CATAAACCCA 
AAACAGAAAT GTTCAACTCT ATACGATTAT TTCTCTCAGT TGTTGGAGGA ACATGGTCCC 
TTGGACATGA GTAACAAAAT GTTTTCTGAA GAATATGAGT TCTTCCCAGA AGAAACTCAA 
CAGATACTAG AAAAAGCAGG AGGCTTGAAA GCGTTTCTCC TGGGATGTCC TCGTTTTGTT 
GTGATTGATA ACTGTATCGC ATTGAAGAAA GTTGCATTAC GGCTCAAGAA AAAGAGAAAG 
AAGAAAAACA TTAAAACAAA AGTAGAAGAA ATTTCAAAAA CAGGAGAGTA TTTACGAGTT 
AAACTACCAC TGAATCCAAC TGCTAGGGAA TTTAAACCAG ATGTAAAGTC CAAACCAGTG 
TCTGACTTAC CTTCAGCACC AGCTTCTGAG GACATGAAGC CTCAGCCTGC ACCTGCTAAT 
TCTCCCCAAT CAGCTCGTGA CACTGGGAAG CCAAAACCAG TTTTCGATAA TTCTTCCAGA 
TCAGTTTCTG AGGATGAGAA GCCCAAAGGG ATGTCTTCTG ATTCTCCCAG GCCAGTCTTT 
GAGGATACCG GTCACAAGCC GGTCTCCTCT AATTCTCCCA AACCAGTTCC TGAGGAAGTG 
AAAGCGACCT ATTGGCCTCG ACCCCAGTTG GTCACAGGAT ACTGTACATA TCTTCCCTTT 
CGGGGATTTG ACATTACCCA GACTCCACCA GCCTTCATCA GTGTGTTACC GACTTTGCCC 
CAGTATAGCA TTTACACCCC TCTGGCCAGT GTTCCTACTG AGTATCACCT GCAAAGACCA 
ATACCAGTGG TGCCGTCTTT TGTAGCCAGT GACATAACGG AAGTGCATTT TGAGGCTCAT 
CATTTCAGTG CTGAGAATGC CCCTGGTACC CAGATTGCCT CCGAAACACA GATCCTTGAG 
GAATCTTTGG AAACACCAGT GAAGTCAGAG TGCAGCTATG ATGGTGCTGA TATAGTCCAG 
AGTGAGGCTA GCAGAAACGA TGACTACTGT GGCGATTCTG CCAAATGGGA AGTAAATCCA 
GAAAGCACCA ATGAAGTAAC AAATATTCCA TGTACACAAG TGGTTGCTGT ACAGGTGTCT 
TGGAATATAA CACATCAGGA AGTCAATACT GAGCCATATG GTCCTTTTGA GGCACAACAG 
GGGGGTATTT CACAGGTTGA AAAGGAGTAC CAGGTTTTAC AAGAGCAGCT TAAGGAAGCA 
TGTGAAAATT ATGAACACAG CAATGTCAAG GGCTCAGAAG AAACCAAGGA CTTGGAAGAA 
AAGTTGAAAA GGAATGTTGA AGAAAATAAG ATCTCAAAGA CAGAACTAGA TTGGTTCCTT 
GAAGATTTGA ACAAGGAAAT TAAAAAATGG GAACAGGAGA AAAAAGAAAT CCAAGAAAGA 
CTAAAGGCAC TGAAGAAGAA AATGAAAAAG GTGTTAAATG CCAGCAAAAT GTATACGCAG 
GAAAATGATG GAAAGGAAAA GGCACTTGAA TTACTTCTGG ATCAGTCCCT TGAAATCAGT 
AATACATTTA CAAATGAGAA AATGAAAATA GAAGAGAGTA TAAAGAAAGG GAAAGAGCAT 
TATGAAGAGA GTCTTCAAAG GGCTGTGGAT GCAGAGGTAT CTGTGCTTGA AAACTGGAAG 
GACATGGAAG TGTGTAAGTT ACAGAGCATG GAGTCACAAG CCGAAAGGTT TCTCAAGAAT 
CTGAAGCTAA TGAGCAGTGA TTCGGCAGCG TATTCTGACA TGGACTCAGA CATACATTCA 
TGGGAATCGT TTCTTTCTGA TGTTAGAGAA GAAATTGAGA AAGCAAAGTC TCAGTTTGAA 
GAACAAATTC AGGCAATTAA ATATGGTTCT CAGCTCAGTG AACTTCCTAA AGTGCAGATT 
TCTGAGCTTT CATTTCCTAC CTGTAACACG ATTCATCCTA AATTACTTCC TGAGTCTTCA 
GGCCAGGATG GTCAAGGTCC CTCCACTTCT ACAAGTCATG GGACCGGAGA CCAGACAGCA 
GCTCCCGAGG AGTCAAGTCT GGGCTCTGCA GCTGACAGCC CAGTGGAGTT GCCCTCCTGC 
AGTCAGAAAG CCCCCCAGCT GCCAGAGCAT GAGGGTGCTG GCCAGGCAAC TGGGCCAGAA 
CCAGGCCCAG TGGCTGATCA GAAGTTACCT GCTGCTCCAG AGCGTGCCAC TCATTCAAGC 
CAGTCTCCAA AGAAGCCGTT CAATAGCATT ATTGAGCACC TGTTGGTGGT GTTCCCGTGT 
TACAGCAGCA CGGAGCTTGC TGGTTTTATT AAAAAAGTTA GAAACAAAAA CAAGACCTCT 
CTGTCTGGAT TGAGTATTGA CGAAATTGTG CAAAGAGTGA CAGAACACGT CCTTGATGAA 
GAAAGAAAGA AAAAGCCAAG TCCAGGAAAG GGCTGGAGGC TGTCTGCACC CAGCTCTGCT 
GTTGCTGTGC CCAGGGCTGC CCAGGACCCG CCCTCCATGC CTGTCGGAGC ATCGCCCGAA 
ACCAGGGGGC CGAGGACCGA GGACGTCCCT GTGTCCTGCG CAAATTCCTG TGAAATGTGC 
CGAGAAGTAT TCAAGTCCAA ACACGTGCGT GAGCTGCAGT GTGGGCACAG GCTTCACAAG 
GGGTGCTTCA AGCAGTGGCT GAAGAGCCAG CGCACCTGCC CAGCCTGCCT GGGTCACGAC 
CTCCTGTCAG AGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005202114.1
CDS	3313..9270
Translation	MADFPQGNFADYALLEDCPYVDDCIFAPEFMSSDYVRVTQLYCDGVGRQYKDYAQTEGNLEFDICTLWCSQPISVLQDYCDAIKVHIFWPLLFQRQQSSIITRMHSCVRTSGSHACEISLKKLQQLELMEDIVDLAKKVVNDTFFIGGLLRIGYKIENKILAMEEALNWVKYTGDVTILSKLGAVDNCWRMLSIFFTEYKYHITKVVTENCSLLEEFKTQSCAECLEQGELMKMKGNEEFSKERYDIAIIYYTRAIECRPENHLLYGNRALCFLRTGQFRNALGDGKRATILKNNWTKGHYRYCAALSMLGEYDWALQANIKAQKLCKNDPEGIKDLIQQHVKLQKQIEDLQGQTPASNPVKAFCESRAYIPSLSAPAFSTSLNFVETEKNFRKTNHEMANGGNQNPKVVDEALKVDDCDCHPEFVPPSSQPPKYKGKQKSRNNELEKFSPGSQETLPVDLKNILEKQFSKSSRAAHQDFANIMKMLRSLIQDGYTALLEQRCRSAAQAFTELLNGLDPQKIKQLNLAMINYVLVVYGLAISLLGIGQPEELSEAENQFKRMIEHYPNEGLDCLAYCGIGKVYLKKNRFLEALNHFEKARTLICRLPGVLTWPTSNVVIEETQPGKIKMLLEKFVEECRFPPVPDATCCYQKCHGYSKIQIYITDPDFKGFIRISCCQYCKIEFHMNCWKKLKTTTFNDKIDKDFLQGICLTPDCEGIISKIIIFSSGGQVKCEFEHKVIKEKVPPRPILKQKCSSLEKLRLKEDKKLKRKLQKKEAKKLAQERLEEDLRESNPPKTEEQRETINHVQSCQFLDDRILQCIKQYADKIKSGILNTSKLLKELLSWKVLTTEDYTTCFSSRNFLNEAVDYVIRHLIQEKNRVKTRVFLHVLSELKEVDPKLATWIQKLNSFGLDATGPFFSRYGAPLKELDFSIMTFLWNEKYGHKLGSIEGKQLDYFYEPTSLKEARCLIWLLEEHRDKFPALHNALDEFFDIMDSRCTVLRKQDSGDVPFNSTKIKNKGKKKKPKDSKPMLVGSGTPSVTPNNETVTSGEDHIKRSLSSAGPFVVPDHLRQDIEEFEALYEQHSNEYVVRNKKLWDINPKQKCSTLYDYFSQLLEEHGPLDMSNKMFSEEYEFFPEETQQILEKAGGLKAFLLGCPRFVVIDNCIALKKVALRLKKKRKKKNIKTKVEEISKTGEYLRVKLPLNPTAREFKPDVKSKPVSDLPSAPASEDMKPQPAPANSPQSARDTGKPKPVFDNSSRSVSEDEKPKGMSSDSPRPVFEDTGHKPVSSNSPKPVPEEVKATYWPRPQLVTGYCTYLPFRGFDITQTPPAFISVLPTLPQYSIYTPLASVPTEYHLQRPIPVVPSFVASDITEVHFEAHHFSAENAPGTQIASETQILEESLETPVKSECSYDGADIVQSEASRNDDYCGDSAKWEVNPESTNEVTNIPCTQVVAVQVSWNITHQEVNTEPYGPFEAQQGGISQVEKEYQVLQEQLKEACENYEHSNVKGSEETKDLEEKLKRNVEENKISKTELDWFLEDLNKEIKKWEQEKKEIQERLKALKKKMKKVLNASKMYTQENDGKEKALELLLDQSLEISNTFTNEKMKIEESIKKGKEHYEESLQRAVDAEVSVLENWKDMEVCKLQSMESQAERFLKNLKLMSSDSAAYSDMDSDIHSWESFLSDVREEIEKAKSQFEEQIQAIKYGSQLSELPKVQISELSFPTCNTIHPKLLPESSGQDGQGPSTSTSHGTGDQTAAPEESSLGSAADSPVELPSCSQKAPQLPEHEGAGQATGPEPGPVADQKLPAAPERATHSSQSPKKPFNSIIEHLLVVFPCYSSTELAGFIKKVRNKNKTSLSGLSIDEIVQRVTEHVLDEERKKKPSPGKGWRLSAPSSAVAVPRAAQDPPSMPVGASPETRGPRTEDVPVSCANSCEMCREVFKSKHVRELQCGHRLHKGCFKQWLKSQRTCPACLGHDLLSEE

Target ORF information:

RefSeq Version	XM_005202057.3
Organism	Bos taurus(cattle)
Definition	Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005202057.3

ORF Insert Sequence:

ATGGCCGATT TTCCCCAGGG AAATTTTGCA GATTATGCCT TGTTAGAAGA TTGCCCTTAT 
GTGGATGATT GTATCTTTGC CCCTGAGTTT ATGTCCAGTG ATTATGTTCG TGTGACTCAG 
CTTTACTGTG ATGGAGTGGG TAGACAGTAT AAAGATTATG CCCAAACTGA GGGAAACTTA 
GAATTTGACA TCTGCACTCT GTGGTGTAGT CAACCTATTT CTGTCCTGCA AGATTACTGT 
GATGCCATTA AAGTCCACAT CTTCTGGCCA CTTCTGTTTC AACGTCAGCA GAGCTCTATA 
ATAACACGGA TGCACTCCTG TGTAAGGACC AGCGGTTCTC ATGCTTGTGA GATAAGTTTG 
AAGAAATTAC AGCAGCTTGA ACTGATGGAA GATATTGTGG ATTTGGCAAA GAAAGTTGTG 
AATGATACAT TCTTTATCGG AGGCTTATTG AGAATTGGCT ATAAAATAGA GAATAAAATC 
TTGGCAATGG AAGAAGCTTT GAATTGGGTG AAATACACAG GCGATGTAAC AATTTTATCT 
AAATTAGGAG CAGTTGATAA TTGTTGGCGT ATGTTAAGTA TTTTCTTTAC TGAATACAAG 
TATCACATAA CTAAAGTTGT AACAGAAAAC TGCAGTTTGC TTGAAGAATT TAAAACTCAA 
AGTTGTGCGG AGTGTCTAGA GCAAGGAGAA CTAATGAAAA TGAAAGGAAA TGAAGAGTTT 
TCCAAAGAAA GATATGATAT AGCTATTATC TATTACACCA GAGCCATTGA ATGTAGGCCT 
GAAAATCACC TGCTTTATGG TAACCGAGCT CTTTGTTTTC TTCGTACTGG ACAATTCAGA 
AATGCACTTG GTGATGGAAA GAGAGCTACC ATTCTGAAGA ACAATTGGAC AAAGGGTCAT 
TATCGTTATT GTGCTGCTCT TTCTATGCTG GGGGAATATG ACTGGGCCCT GCAAGCAAAC 
ATAAAAGCTC AAAAACTCTG TAAAAATGAC CCTGAGGGAA TCAAGGATCT AATTCAGCAG 
CATGTAAAGT TACAAAAACA AATAGAAGAC CTGCAAGGTC AAACACCAGC TAGTAATCCA 
GTCAAAGCCT TCTGTGAAAG CAGGGCCTAC ATACCTAGTT TATCAGCACC TGCATTTAGT 
ACTTCACTTA ACTTTGTGGA AACTGAAAAA AATTTCAGAA AAACTAACCA CGAAATGGCA 
AACGGTGGTA ATCAGAATCC AAAGGTGGTA GATGAAGCTC TGAAAGTAGA TGATTGTGAC 
TGTCATCCTG AATTTGTACC GCCATCAAGT CAGCCTCCAA AATATAAAGG GAAACAAAAA 
TCTCGAAACA ATGAATTGGA GAAGTTCAGC CCTGGTTCAC AAGAAACTTT ACCAGTAGAT 
TTAAAAAACA TCTTGGAGAA ACAGTTTTCT AAATCTTCCA GAGCCGCACA CCAGGATTTT 
GCTAATATAA TGAAAATGCT GAGAAGTTTA ATTCAGGATG GCTACACGGC CTTGCTGGAG 
CAGCGCTGCC GCAGCGCCGC CCAAGCCTTC ACAGAGTTGC TCAACGGTTT AGATCCTCAA 
AAAATAAAGC AATTGAACCT GGCCATGATT AACTATGTGT TAGTAGTCTA CGGACTTGCC 
ATTTCACTCC TTGGAATAGG ACAGCCCGAG GAGTTATCTG AAGCTGAAAA CCAGTTCAAG 
AGGATGATTG AACACTACCC CAATGAGGGG CTTGATTGCT TGGCCTACTG TGGAATTGGA 
AAAGTGTATT TGAAAAAAAA CAGATTTTTA GAAGCTCTTA ATCATTTTGA GAAAGCAAGA 
ACCTTGATCT GCCGTCTTCC TGGAGTGTTA ACTTGGCCCA CAAGTAATGT GGTTATTGAG 
GAGACTCAGC CAGGGAAGAT AAAGATGCTG TTAGAGAAAT TTGTTGAAGA ATGCAGGTTC 
CCTCCAGTGC CGGATGCCAC TTGTTGCTAT CAGAAGTGCC ATGGATATTC CAAAATCCAG 
ATATATATAA CTGATCCAGA CTTTAAGGGT TTTATACGGA TCAGCTGTTG CCAGTATTGT 
AAAATAGAAT TTCACATGAA CTGCTGGAAG AAGTTAAAAA CAACAACCTT CAATGATAAA 
ATTGACAAGG ATTTTCTGCA AGGAATCTGT CTTACTCCTG ATTGTGAAGG AATCATTTCA 
AAGATTATCA TCTTCAGCAG TGGTGGTCAA GTTAAATGTG AATTTGAACA CAAGGTCATT 
AAAGAAAAGG TTCCTCCAAG ACCTATTCTG AAACAGAAAT GTTCTAGCCT AGAGAAGTTG 
AGGCTGAAAG AAGACAAAAA ATTGAAGCGA AAGCTCCAAA AAAAGGAAGC AAAAAAATTA 
GCACAAGAAA GACTGGAAGA GGACTTAAGG GAAAGTAACC CACCAAAAAC TGAAGAGCAG 
AGAGAAACTA TCAACCATGT CCAAAGTTGC CAGTTCCTTG ATGACAGAAT TCTGCAGTGC 
ATAAAGCAGT ACGCTGACAA GATTAAGTCC GGGATACTGA ATACTTCCAA GCTTCTCAAA 
GAATTGCTTT CTTGGAAGGT TTTAACCACA GAAGACTACA CAACGTGTTT TTCTAGCAGA 
AATTTCCTGA ATGAAGCAGT GGACTATGTC ATTCGTCATT TGATCCAAGA AAAGAATAGA 
GTAAAGACAA GGGTATTTCT GCATGTTTTG AGTGAGCTTA AAGAAGTGGA CCCAAAACTA 
GCCACCTGGA TCCAGAAACT GAATAGCTTT GGCTTAGATG CCACAGGACC TTTCTTTTCT 
CGTTATGGGG CTCCTCTTAA GGAGCTTGAT TTTAGCATCA TGACTTTTCT CTGGAATGAG 
AAATACGGTC ATAAGCTAGG CTCTATAGAA GGAAAGCAAC TTGATTATTT CTATGAGCCT 
ACATCACTGA AGGAAGCGCG GTGTTTAATA TGGCTGCTAG AAGAACACAG AGACAAGTTC 
CCAGCACTGC ATAATGCCTT GGACGAGTTC TTTGATATAA TGGACAGCCG CTGCACTGTA 
TTAAGGAAAC AGGACAGTGG TGACGTGCCA TTTAATTCTA CCAAGATAAA AAACAAAGGC 
AAGAAAAAGA AGCCAAAAGA TTCAAAGCCT ATGTTAGTTG GATCTGGAAC ACCTTCAGTC 
ACACCAAACA ATGAGACTGT CACTTCAGGT GAAGACCATA TCAAACGCAG TTTAAGTTCT 
GCGGGCCCCT TTGTAGTGCC TGATCACCTT CGGCAGGATA TAGAAGAATT TGAAGCTCTG 
TATGAACAGC ATAGTAATGA GTATGTTGTC CGTAATAAGA AGCTGTGGGA CATAAACCCA 
AAACAGAAAT GTTCAACTCT ATACGATTAT TTCTCTCAGT TGTTGGAGGA ACATGGTCCC 
TTGGACATGA GTAACAAAAT GTTTTCTGAA GAATATGAGT TCTTCCCAGA AGAAACTCAA 
CAGATACTAG AAAAAGCAGG AGGCTTGAAA GCGTTTCTCC TGGGATGTCC TCGTTTTGTT 
GTGATTGATA ACTGTATCGC ATTGAAGAAA GTTGCATTAC GGCTCAAGAA AAAGAGAAAG 
AAGAAAAACA TTAAAACAAA AGTAGAAGAA ATTTCAAAAA CAGGAGAGTA TTTACGAGTT 
AAACTACCAC TGAATCCAAC TGCTAGGGAA TTTAAACCAG ATGTAAAGTC CAAACCAGTG 
TCTGACTTAC CTTCAGCACC AGCTTCTGAG GACATGAAGC CTCAGCCTGC ACCTGCTAAT 
TCTCCCCAAT CAGCTCGTGA CACTGGGAAG CCAAAACCAG TTTTCGATAA TTCTTCCAGA 
TCAGTTTCTG AGGATGAGAA GCCCAAAGGG ATGTCTTCTG ATTCTCCCAG GCCAGTCTTT 
GAGGATACCG GTCACAAGCC GGTCTCCTCT AATTCTCCCA AACCAGTTCC TGAGGAAGTG 
AAAGCGACCT ATTGGCCTCG ACCCCAGTTG GTCACAGGAT ACTGTACATA TCTTCCCTTT 
CGGGGATTTG ACATTACCCA GACTCCACCA GCCTTCATCA GTGTGTTACC GACTTTGCCC 
CAGTATAGCA TTTACACCCC TCTGGCCAGT GTTCCTACTG AGTATCACCT GCAAAGACCA 
ATACCAGTGG TGCCGTCTTT TGTAGCCAGT GACATAACGG AAGTGCATTT TGAGGCTCAT 
CATTTCAGTG CTGAGAATGC CCCTGGTACC CAGATTGCCT CCGAAACACA GATCCTTGAG 
GAATCTTTGG AAACACCAGT GAAGTCAGAG TGCAGCTATG ATGGTGCTGA TATAGTCCAG 
AGTGAGGCTA GCAGAAACGA TGACTACTGT GGCGATTCTG CCAAATGGGA AGTAAATCCA 
GAAAGCACCA ATGAAGTAAC AAATATTCCA TGTACACAAG TGGTTGCTGT ACAGGTGTCT 
TGGAATATAA CACATCAGGA AGTCAATACT GAGCCATATG GTCCTTTTGA GGCACAACAG 
GGGGGTATTT CACAGGTTGA AAAGGAGTAC CAGGTTTTAC AAGAGCAGCT TAAGGAAGCA 
TGTGAAAATT ATGAACACAG CAATGTCAAG GGCTCAGAAG AAACCAAGGA CTTGGAAGAA 
AAGTTGAAAA GGAATGTTGA AGAAAATAAG ATCTCAAAGA CAGAACTAGA TTGGTTCCTT 
GAAGATTTGA ACAAGGAAAT TAAAAAATGG GAACAGGAGA AAAAAGAAAT CCAAGAAAGA 
CTAAAGGCAC TGAAGAAGAA AATGAAAAAG GTGTTAAATG CCAGCAAAAT GTATACGCAG 
GAAAATGATG GAAAGGAAAA GGCACTTGAA TTACTTCTGG ATCAGTCCCT TGAAATCAGT 
AATACATTTA CAAATGAGAA AATGAAAATA GAAGAGAGTA TAAAGAAAGG GAAAGAGCAT 
TATGAAGAGA GTCTTCAAAG GGCTGTGGAT GCAGAGGTAT CTGTGCTTGA AAACTGGAAG 
GACATGGAAG TGTGTAAGTT ACAGAGCATG GAGTCACAAG CCGAAAGGTT TCTCAAGAAT 
CTGAAGCTAA TGAGCAGTGA TTCGGCAGCG TATTCTGACA TGGACTCAGA CATACATTCA 
TGGGAATCGT TTCTTTCTGA TGTTAGAGAA GAAATTGAGA AAGCAAAGTC TCAGTTTGAA 
GAACAAATTC AGGCAATTAA ATATGGTTCT CAGCTCAGTG AACTTCCTAA AGTGCAGATT 
TCTGAGCTTT CATTTCCTAC CTGTAACACG ATTCATCCTA AATTACTTCC TGAGTCTTCA 
GGCCAGGATG GTCAAGGTCC CTCCACTTCT ACAAGTCATG GGACCGGAGA CCAGACAGCA 
GCTCCCGAGG AGTCAAGTCT GGGCTCTGCA GCTGACAGCC CAGTGGAGTT GCCCTCCTGC 
AGTCAGAAAG CCCCCCAGCT GCCAGAGCAT GAGGGTGCTG GCCAGGCAAC TGGGCCAGAA 
CCAGGCCCAG TGGCTGATCA GAAGTTACCT GCTGCTCCAG AGCGTGCCAC TCATTCAAGC 
CAGTCTCCAA AGAAGCCGTT CAATAGCATT ATTGAGCACC TGTTGGTGGT GTTCCCGTGT 
TACAGCAGCA CGGAGCTTGC TGGTTTTATT AAAAAAGTTA GAAACAAAAA CAAGACCTCT 
CTGTCTGGAT TGAGTATTGA CGAAATTGTG CAAAGAGTGA CAGAACACGT CCTTGATGAA 
GAAAGAAAGA AAAAGCCAAG TCCAGGAAAG GGCTGGAGGC TGTCTGCACC CAGCTCTGCT 
GTTGCTGTGC CCAGGGCTGC CCAGGACCCG CCCTCCATGC CTGTCGGAGC ATCGCCCGAA 
ACCAGGGGGC CGAGGACCGA GGACGTCCCT GTGTCCTGCG CAAATTCCTG TGAAATGTGC 
CGAGAAGTAT TCAAGTCCAA ACACGTGCGT GAGCTGCAGT GTGGGCACAG GCTTCACAAG 
GGGTGCTTCA AGCAGTGGCT GAAGAGCCAG CGCACCTGCC CAGCCTGCCT GGGTCACGAC 
CTCCTGTCAG AGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_005202056.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005202113.1 Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X1, mRNA.

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CloneID	OBa16127
Clone ID Related Accession (Same CDS sequence)	XM_005202058.3 , XM_005202057.3 , NM_001109767.3 , XM_005202056.3
Accession Version	XM_005202056.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5958bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TTC3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103826.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005202056.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGATT TTCCCCAGGG AAATTTTGCA GATTATGCCT TGTTAGAAGA TTGCCCTTAT 
GTGGATGATT GTATCTTTGC CCCTGAGTTT ATGTCCAGTG ATTATGTTCG TGTGACTCAG 
CTTTACTGTG ATGGAGTGGG TAGACAGTAT AAAGATTATG CCCAAACTGA GGGAAACTTA 
GAATTTGACA TCTGCACTCT GTGGTGTAGT CAACCTATTT CTGTCCTGCA AGATTACTGT 
GATGCCATTA AAGTCCACAT CTTCTGGCCA CTTCTGTTTC AACGTCAGCA GAGCTCTATA 
ATAACACGGA TGCACTCCTG TGTAAGGACC AGCGGTTCTC ATGCTTGTGA GATAAGTTTG 
AAGAAATTAC AGCAGCTTGA ACTGATGGAA GATATTGTGG ATTTGGCAAA GAAAGTTGTG 
AATGATACAT TCTTTATCGG AGGCTTATTG AGAATTGGCT ATAAAATAGA GAATAAAATC 
TTGGCAATGG AAGAAGCTTT GAATTGGGTG AAATACACAG GCGATGTAAC AATTTTATCT 
AAATTAGGAG CAGTTGATAA TTGTTGGCGT ATGTTAAGTA TTTTCTTTAC TGAATACAAG 
TATCACATAA CTAAAGTTGT AACAGAAAAC TGCAGTTTGC TTGAAGAATT TAAAACTCAA 
AGTTGTGCGG AGTGTCTAGA GCAAGGAGAA CTAATGAAAA TGAAAGGAAA TGAAGAGTTT 
TCCAAAGAAA GATATGATAT AGCTATTATC TATTACACCA GAGCCATTGA ATGTAGGCCT 
GAAAATCACC TGCTTTATGG TAACCGAGCT CTTTGTTTTC TTCGTACTGG ACAATTCAGA 
AATGCACTTG GTGATGGAAA GAGAGCTACC ATTCTGAAGA ACAATTGGAC AAAGGGTCAT 
TATCGTTATT GTGCTGCTCT TTCTATGCTG GGGGAATATG ACTGGGCCCT GCAAGCAAAC 
ATAAAAGCTC AAAAACTCTG TAAAAATGAC CCTGAGGGAA TCAAGGATCT AATTCAGCAG 
CATGTAAAGT TACAAAAACA AATAGAAGAC CTGCAAGGTC AAACACCAGC TAGTAATCCA 
GTCAAAGCCT TCTGTGAAAG CAGGGCCTAC ATACCTAGTT TATCAGCACC TGCATTTAGT 
ACTTCACTTA ACTTTGTGGA AACTGAAAAA AATTTCAGAA AAACTAACCA CGAAATGGCA 
AACGGTGGTA ATCAGAATCC AAAGGTGGTA GATGAAGCTC TGAAAGTAGA TGATTGTGAC 
TGTCATCCTG AATTTGTACC GCCATCAAGT CAGCCTCCAA AATATAAAGG GAAACAAAAA 
TCTCGAAACA ATGAATTGGA GAAGTTCAGC CCTGGTTCAC AAGAAACTTT ACCAGTAGAT 
TTAAAAAACA TCTTGGAGAA ACAGTTTTCT AAATCTTCCA GAGCCGCACA CCAGGATTTT 
GCTAATATAA TGAAAATGCT GAGAAGTTTA ATTCAGGATG GCTACACGGC CTTGCTGGAG 
CAGCGCTGCC GCAGCGCCGC CCAAGCCTTC ACAGAGTTGC TCAACGGTTT AGATCCTCAA 
AAAATAAAGC AATTGAACCT GGCCATGATT AACTATGTGT TAGTAGTCTA CGGACTTGCC 
ATTTCACTCC TTGGAATAGG ACAGCCCGAG GAGTTATCTG AAGCTGAAAA CCAGTTCAAG 
AGGATGATTG AACACTACCC CAATGAGGGG CTTGATTGCT TGGCCTACTG TGGAATTGGA 
AAAGTGTATT TGAAAAAAAA CAGATTTTTA GAAGCTCTTA ATCATTTTGA GAAAGCAAGA 
ACCTTGATCT GCCGTCTTCC TGGAGTGTTA ACTTGGCCCA CAAGTAATGT GGTTATTGAG 
GAGACTCAGC CAGGGAAGAT AAAGATGCTG TTAGAGAAAT TTGTTGAAGA ATGCAGGTTC 
CCTCCAGTGC CGGATGCCAC TTGTTGCTAT CAGAAGTGCC ATGGATATTC CAAAATCCAG 
ATATATATAA CTGATCCAGA CTTTAAGGGT TTTATACGGA TCAGCTGTTG CCAGTATTGT 
AAAATAGAAT TTCACATGAA CTGCTGGAAG AAGTTAAAAA CAACAACCTT CAATGATAAA 
ATTGACAAGG ATTTTCTGCA AGGAATCTGT CTTACTCCTG ATTGTGAAGG AATCATTTCA 
AAGATTATCA TCTTCAGCAG TGGTGGTCAA GTTAAATGTG AATTTGAACA CAAGGTCATT 
AAAGAAAAGG TTCCTCCAAG ACCTATTCTG AAACAGAAAT GTTCTAGCCT AGAGAAGTTG 
AGGCTGAAAG AAGACAAAAA ATTGAAGCGA AAGCTCCAAA AAAAGGAAGC AAAAAAATTA 
GCACAAGAAA GACTGGAAGA GGACTTAAGG GAAAGTAACC CACCAAAAAC TGAAGAGCAG 
AGAGAAACTA TCAACCATGT CCAAAGTTGC CAGTTCCTTG ATGACAGAAT TCTGCAGTGC 
ATAAAGCAGT ACGCTGACAA GATTAAGTCC GGGATACTGA ATACTTCCAA GCTTCTCAAA 
GAATTGCTTT CTTGGAAGGT TTTAACCACA GAAGACTACA CAACGTGTTT TTCTAGCAGA 
AATTTCCTGA ATGAAGCAGT GGACTATGTC ATTCGTCATT TGATCCAAGA AAAGAATAGA 
GTAAAGACAA GGGTATTTCT GCATGTTTTG AGTGAGCTTA AAGAAGTGGA CCCAAAACTA 
GCCACCTGGA TCCAGAAACT GAATAGCTTT GGCTTAGATG CCACAGGACC TTTCTTTTCT 
CGTTATGGGG CTCCTCTTAA GGAGCTTGAT TTTAGCATCA TGACTTTTCT CTGGAATGAG 
AAATACGGTC ATAAGCTAGG CTCTATAGAA GGAAAGCAAC TTGATTATTT CTATGAGCCT 
ACATCACTGA AGGAAGCGCG GTGTTTAATA TGGCTGCTAG AAGAACACAG AGACAAGTTC 
CCAGCACTGC ATAATGCCTT GGACGAGTTC TTTGATATAA TGGACAGCCG CTGCACTGTA 
TTAAGGAAAC AGGACAGTGG TGACGTGCCA TTTAATTCTA CCAAGATAAA AAACAAAGGC 
AAGAAAAAGA AGCCAAAAGA TTCAAAGCCT ATGTTAGTTG GATCTGGAAC ACCTTCAGTC 
ACACCAAACA ATGAGACTGT CACTTCAGGT GAAGACCATA TCAAACGCAG TTTAAGTTCT 
GCGGGCCCCT TTGTAGTGCC TGATCACCTT CGGCAGGATA TAGAAGAATT TGAAGCTCTG 
TATGAACAGC ATAGTAATGA GTATGTTGTC CGTAATAAGA AGCTGTGGGA CATAAACCCA 
AAACAGAAAT GTTCAACTCT ATACGATTAT TTCTCTCAGT TGTTGGAGGA ACATGGTCCC 
TTGGACATGA GTAACAAAAT GTTTTCTGAA GAATATGAGT TCTTCCCAGA AGAAACTCAA 
CAGATACTAG AAAAAGCAGG AGGCTTGAAA GCGTTTCTCC TGGGATGTCC TCGTTTTGTT 
GTGATTGATA ACTGTATCGC ATTGAAGAAA GTTGCATTAC GGCTCAAGAA AAAGAGAAAG 
AAGAAAAACA TTAAAACAAA AGTAGAAGAA ATTTCAAAAA CAGGAGAGTA TTTACGAGTT 
AAACTACCAC TGAATCCAAC TGCTAGGGAA TTTAAACCAG ATGTAAAGTC CAAACCAGTG 
TCTGACTTAC CTTCAGCACC AGCTTCTGAG GACATGAAGC CTCAGCCTGC ACCTGCTAAT 
TCTCCCCAAT CAGCTCGTGA CACTGGGAAG CCAAAACCAG TTTTCGATAA TTCTTCCAGA 
TCAGTTTCTG AGGATGAGAA GCCCAAAGGG ATGTCTTCTG ATTCTCCCAG GCCAGTCTTT 
GAGGATACCG GTCACAAGCC GGTCTCCTCT AATTCTCCCA AACCAGTTCC TGAGGAAGTG 
AAAGCGACCT ATTGGCCTCG ACCCCAGTTG GTCACAGGAT ACTGTACATA TCTTCCCTTT 
CGGGGATTTG ACATTACCCA GACTCCACCA GCCTTCATCA GTGTGTTACC GACTTTGCCC 
CAGTATAGCA TTTACACCCC TCTGGCCAGT GTTCCTACTG AGTATCACCT GCAAAGACCA 
ATACCAGTGG TGCCGTCTTT TGTAGCCAGT GACATAACGG AAGTGCATTT TGAGGCTCAT 
CATTTCAGTG CTGAGAATGC CCCTGGTACC CAGATTGCCT CCGAAACACA GATCCTTGAG 
GAATCTTTGG AAACACCAGT GAAGTCAGAG TGCAGCTATG ATGGTGCTGA TATAGTCCAG 
AGTGAGGCTA GCAGAAACGA TGACTACTGT GGCGATTCTG CCAAATGGGA AGTAAATCCA 
GAAAGCACCA ATGAAGTAAC AAATATTCCA TGTACACAAG TGGTTGCTGT ACAGGTGTCT 
TGGAATATAA CACATCAGGA AGTCAATACT GAGCCATATG GTCCTTTTGA GGCACAACAG 
GGGGGTATTT CACAGGTTGA AAAGGAGTAC CAGGTTTTAC AAGAGCAGCT TAAGGAAGCA 
TGTGAAAATT ATGAACACAG CAATGTCAAG GGCTCAGAAG AAACCAAGGA CTTGGAAGAA 
AAGTTGAAAA GGAATGTTGA AGAAAATAAG ATCTCAAAGA CAGAACTAGA TTGGTTCCTT 
GAAGATTTGA ACAAGGAAAT TAAAAAATGG GAACAGGAGA AAAAAGAAAT CCAAGAAAGA 
CTAAAGGCAC TGAAGAAGAA AATGAAAAAG GTGTTAAATG CCAGCAAAAT GTATACGCAG 
GAAAATGATG GAAAGGAAAA GGCACTTGAA TTACTTCTGG ATCAGTCCCT TGAAATCAGT 
AATACATTTA CAAATGAGAA AATGAAAATA GAAGAGAGTA TAAAGAAAGG GAAAGAGCAT 
TATGAAGAGA GTCTTCAAAG GGCTGTGGAT GCAGAGGTAT CTGTGCTTGA AAACTGGAAG 
GACATGGAAG TGTGTAAGTT ACAGAGCATG GAGTCACAAG CCGAAAGGTT TCTCAAGAAT 
CTGAAGCTAA TGAGCAGTGA TTCGGCAGCG TATTCTGACA TGGACTCAGA CATACATTCA 
TGGGAATCGT TTCTTTCTGA TGTTAGAGAA GAAATTGAGA AAGCAAAGTC TCAGTTTGAA 
GAACAAATTC AGGCAATTAA ATATGGTTCT CAGCTCAGTG AACTTCCTAA AGTGCAGATT 
TCTGAGCTTT CATTTCCTAC CTGTAACACG ATTCATCCTA AATTACTTCC TGAGTCTTCA 
GGCCAGGATG GTCAAGGTCC CTCCACTTCT ACAAGTCATG GGACCGGAGA CCAGACAGCA 
GCTCCCGAGG AGTCAAGTCT GGGCTCTGCA GCTGACAGCC CAGTGGAGTT GCCCTCCTGC 
AGTCAGAAAG CCCCCCAGCT GCCAGAGCAT GAGGGTGCTG GCCAGGCAAC TGGGCCAGAA 
CCAGGCCCAG TGGCTGATCA GAAGTTACCT GCTGCTCCAG AGCGTGCCAC TCATTCAAGC 
CAGTCTCCAA AGAAGCCGTT CAATAGCATT ATTGAGCACC TGTTGGTGGT GTTCCCGTGT 
TACAGCAGCA CGGAGCTTGC TGGTTTTATT AAAAAAGTTA GAAACAAAAA CAAGACCTCT 
CTGTCTGGAT TGAGTATTGA CGAAATTGTG CAAAGAGTGA CAGAACACGT CCTTGATGAA 
GAAAGAAAGA AAAAGCCAAG TCCAGGAAAG GGCTGGAGGC TGTCTGCACC CAGCTCTGCT 
GTTGCTGTGC CCAGGGCTGC CCAGGACCCG CCCTCCATGC CTGTCGGAGC ATCGCCCGAA 
ACCAGGGGGC CGAGGACCGA GGACGTCCCT GTGTCCTGCG CAAATTCCTG TGAAATGTGC 
CGAGAAGTAT TCAAGTCCAA ACACGTGCGT GAGCTGCAGT GTGGGCACAG GCTTCACAAG 
GGGTGCTTCA AGCAGTGGCT GAAGAGCCAG CGCACCTGCC CAGCCTGCCT GGGTCACGAC 
CTCCTGTCAG AGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005202113.1
CDS	115..6072
Translation	MADFPQGNFADYALLEDCPYVDDCIFAPEFMSSDYVRVTQLYCDGVGRQYKDYAQTEGNLEFDICTLWCSQPISVLQDYCDAIKVHIFWPLLFQRQQSSIITRMHSCVRTSGSHACEISLKKLQQLELMEDIVDLAKKVVNDTFFIGGLLRIGYKIENKILAMEEALNWVKYTGDVTILSKLGAVDNCWRMLSIFFTEYKYHITKVVTENCSLLEEFKTQSCAECLEQGELMKMKGNEEFSKERYDIAIIYYTRAIECRPENHLLYGNRALCFLRTGQFRNALGDGKRATILKNNWTKGHYRYCAALSMLGEYDWALQANIKAQKLCKNDPEGIKDLIQQHVKLQKQIEDLQGQTPASNPVKAFCESRAYIPSLSAPAFSTSLNFVETEKNFRKTNHEMANGGNQNPKVVDEALKVDDCDCHPEFVPPSSQPPKYKGKQKSRNNELEKFSPGSQETLPVDLKNILEKQFSKSSRAAHQDFANIMKMLRSLIQDGYTALLEQRCRSAAQAFTELLNGLDPQKIKQLNLAMINYVLVVYGLAISLLGIGQPEELSEAENQFKRMIEHYPNEGLDCLAYCGIGKVYLKKNRFLEALNHFEKARTLICRLPGVLTWPTSNVVIEETQPGKIKMLLEKFVEECRFPPVPDATCCYQKCHGYSKIQIYITDPDFKGFIRISCCQYCKIEFHMNCWKKLKTTTFNDKIDKDFLQGICLTPDCEGIISKIIIFSSGGQVKCEFEHKVIKEKVPPRPILKQKCSSLEKLRLKEDKKLKRKLQKKEAKKLAQERLEEDLRESNPPKTEEQRETINHVQSCQFLDDRILQCIKQYADKIKSGILNTSKLLKELLSWKVLTTEDYTTCFSSRNFLNEAVDYVIRHLIQEKNRVKTRVFLHVLSELKEVDPKLATWIQKLNSFGLDATGPFFSRYGAPLKELDFSIMTFLWNEKYGHKLGSIEGKQLDYFYEPTSLKEARCLIWLLEEHRDKFPALHNALDEFFDIMDSRCTVLRKQDSGDVPFNSTKIKNKGKKKKPKDSKPMLVGSGTPSVTPNNETVTSGEDHIKRSLSSAGPFVVPDHLRQDIEEFEALYEQHSNEYVVRNKKLWDINPKQKCSTLYDYFSQLLEEHGPLDMSNKMFSEEYEFFPEETQQILEKAGGLKAFLLGCPRFVVIDNCIALKKVALRLKKKRKKKNIKTKVEEISKTGEYLRVKLPLNPTAREFKPDVKSKPVSDLPSAPASEDMKPQPAPANSPQSARDTGKPKPVFDNSSRSVSEDEKPKGMSSDSPRPVFEDTGHKPVSSNSPKPVPEEVKATYWPRPQLVTGYCTYLPFRGFDITQTPPAFISVLPTLPQYSIYTPLASVPTEYHLQRPIPVVPSFVASDITEVHFEAHHFSAENAPGTQIASETQILEESLETPVKSECSYDGADIVQSEASRNDDYCGDSAKWEVNPESTNEVTNIPCTQVVAVQVSWNITHQEVNTEPYGPFEAQQGGISQVEKEYQVLQEQLKEACENYEHSNVKGSEETKDLEEKLKRNVEENKISKTELDWFLEDLNKEIKKWEQEKKEIQERLKALKKKMKKVLNASKMYTQENDGKEKALELLLDQSLEISNTFTNEKMKIEESIKKGKEHYEESLQRAVDAEVSVLENWKDMEVCKLQSMESQAERFLKNLKLMSSDSAAYSDMDSDIHSWESFLSDVREEIEKAKSQFEEQIQAIKYGSQLSELPKVQISELSFPTCNTIHPKLLPESSGQDGQGPSTSTSHGTGDQTAAPEESSLGSAADSPVELPSCSQKAPQLPEHEGAGQATGPEPGPVADQKLPAAPERATHSSQSPKKPFNSIIEHLLVVFPCYSSTELAGFIKKVRNKNKTSLSGLSIDEIVQRVTEHVLDEERKKKPSPGKGWRLSAPSSAVAVPRAAQDPPSMPVGASPETRGPRTEDVPVSCANSCEMCREVFKSKHVRELQCGHRLHKGCFKQWLKSQRTCPACLGHDLLSEE

Target ORF information:

RefSeq Version	XM_005202056.3
Organism	Bos taurus(cattle)
Definition	Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005202056.3

ORF Insert Sequence:

ATGGCCGATT TTCCCCAGGG AAATTTTGCA GATTATGCCT TGTTAGAAGA TTGCCCTTAT 
GTGGATGATT GTATCTTTGC CCCTGAGTTT ATGTCCAGTG ATTATGTTCG TGTGACTCAG 
CTTTACTGTG ATGGAGTGGG TAGACAGTAT AAAGATTATG CCCAAACTGA GGGAAACTTA 
GAATTTGACA TCTGCACTCT GTGGTGTAGT CAACCTATTT CTGTCCTGCA AGATTACTGT 
GATGCCATTA AAGTCCACAT CTTCTGGCCA CTTCTGTTTC AACGTCAGCA GAGCTCTATA 
ATAACACGGA TGCACTCCTG TGTAAGGACC AGCGGTTCTC ATGCTTGTGA GATAAGTTTG 
AAGAAATTAC AGCAGCTTGA ACTGATGGAA GATATTGTGG ATTTGGCAAA GAAAGTTGTG 
AATGATACAT TCTTTATCGG AGGCTTATTG AGAATTGGCT ATAAAATAGA GAATAAAATC 
TTGGCAATGG AAGAAGCTTT GAATTGGGTG AAATACACAG GCGATGTAAC AATTTTATCT 
AAATTAGGAG CAGTTGATAA TTGTTGGCGT ATGTTAAGTA TTTTCTTTAC TGAATACAAG 
TATCACATAA CTAAAGTTGT AACAGAAAAC TGCAGTTTGC TTGAAGAATT TAAAACTCAA 
AGTTGTGCGG AGTGTCTAGA GCAAGGAGAA CTAATGAAAA TGAAAGGAAA TGAAGAGTTT 
TCCAAAGAAA GATATGATAT AGCTATTATC TATTACACCA GAGCCATTGA ATGTAGGCCT 
GAAAATCACC TGCTTTATGG TAACCGAGCT CTTTGTTTTC TTCGTACTGG ACAATTCAGA 
AATGCACTTG GTGATGGAAA GAGAGCTACC ATTCTGAAGA ACAATTGGAC AAAGGGTCAT 
TATCGTTATT GTGCTGCTCT TTCTATGCTG GGGGAATATG ACTGGGCCCT GCAAGCAAAC 
ATAAAAGCTC AAAAACTCTG TAAAAATGAC CCTGAGGGAA TCAAGGATCT AATTCAGCAG 
CATGTAAAGT TACAAAAACA AATAGAAGAC CTGCAAGGTC AAACACCAGC TAGTAATCCA 
GTCAAAGCCT TCTGTGAAAG CAGGGCCTAC ATACCTAGTT TATCAGCACC TGCATTTAGT 
ACTTCACTTA ACTTTGTGGA AACTGAAAAA AATTTCAGAA AAACTAACCA CGAAATGGCA 
AACGGTGGTA ATCAGAATCC AAAGGTGGTA GATGAAGCTC TGAAAGTAGA TGATTGTGAC 
TGTCATCCTG AATTTGTACC GCCATCAAGT CAGCCTCCAA AATATAAAGG GAAACAAAAA 
TCTCGAAACA ATGAATTGGA GAAGTTCAGC CCTGGTTCAC AAGAAACTTT ACCAGTAGAT 
TTAAAAAACA TCTTGGAGAA ACAGTTTTCT AAATCTTCCA GAGCCGCACA CCAGGATTTT 
GCTAATATAA TGAAAATGCT GAGAAGTTTA ATTCAGGATG GCTACACGGC CTTGCTGGAG 
CAGCGCTGCC GCAGCGCCGC CCAAGCCTTC ACAGAGTTGC TCAACGGTTT AGATCCTCAA 
AAAATAAAGC AATTGAACCT GGCCATGATT AACTATGTGT TAGTAGTCTA CGGACTTGCC 
ATTTCACTCC TTGGAATAGG ACAGCCCGAG GAGTTATCTG AAGCTGAAAA CCAGTTCAAG 
AGGATGATTG AACACTACCC CAATGAGGGG CTTGATTGCT TGGCCTACTG TGGAATTGGA 
AAAGTGTATT TGAAAAAAAA CAGATTTTTA GAAGCTCTTA ATCATTTTGA GAAAGCAAGA 
ACCTTGATCT GCCGTCTTCC TGGAGTGTTA ACTTGGCCCA CAAGTAATGT GGTTATTGAG 
GAGACTCAGC CAGGGAAGAT AAAGATGCTG TTAGAGAAAT TTGTTGAAGA ATGCAGGTTC 
CCTCCAGTGC CGGATGCCAC TTGTTGCTAT CAGAAGTGCC ATGGATATTC CAAAATCCAG 
ATATATATAA CTGATCCAGA CTTTAAGGGT TTTATACGGA TCAGCTGTTG CCAGTATTGT 
AAAATAGAAT TTCACATGAA CTGCTGGAAG AAGTTAAAAA CAACAACCTT CAATGATAAA 
ATTGACAAGG ATTTTCTGCA AGGAATCTGT CTTACTCCTG ATTGTGAAGG AATCATTTCA 
AAGATTATCA TCTTCAGCAG TGGTGGTCAA GTTAAATGTG AATTTGAACA CAAGGTCATT 
AAAGAAAAGG TTCCTCCAAG ACCTATTCTG AAACAGAAAT GTTCTAGCCT AGAGAAGTTG 
AGGCTGAAAG AAGACAAAAA ATTGAAGCGA AAGCTCCAAA AAAAGGAAGC AAAAAAATTA 
GCACAAGAAA GACTGGAAGA GGACTTAAGG GAAAGTAACC CACCAAAAAC TGAAGAGCAG 
AGAGAAACTA TCAACCATGT CCAAAGTTGC CAGTTCCTTG ATGACAGAAT TCTGCAGTGC 
ATAAAGCAGT ACGCTGACAA GATTAAGTCC GGGATACTGA ATACTTCCAA GCTTCTCAAA 
GAATTGCTTT CTTGGAAGGT TTTAACCACA GAAGACTACA CAACGTGTTT TTCTAGCAGA 
AATTTCCTGA ATGAAGCAGT GGACTATGTC ATTCGTCATT TGATCCAAGA AAAGAATAGA 
GTAAAGACAA GGGTATTTCT GCATGTTTTG AGTGAGCTTA AAGAAGTGGA CCCAAAACTA 
GCCACCTGGA TCCAGAAACT GAATAGCTTT GGCTTAGATG CCACAGGACC TTTCTTTTCT 
CGTTATGGGG CTCCTCTTAA GGAGCTTGAT TTTAGCATCA TGACTTTTCT CTGGAATGAG 
AAATACGGTC ATAAGCTAGG CTCTATAGAA GGAAAGCAAC TTGATTATTT CTATGAGCCT 
ACATCACTGA AGGAAGCGCG GTGTTTAATA TGGCTGCTAG AAGAACACAG AGACAAGTTC 
CCAGCACTGC ATAATGCCTT GGACGAGTTC TTTGATATAA TGGACAGCCG CTGCACTGTA 
TTAAGGAAAC AGGACAGTGG TGACGTGCCA TTTAATTCTA CCAAGATAAA AAACAAAGGC 
AAGAAAAAGA AGCCAAAAGA TTCAAAGCCT ATGTTAGTTG GATCTGGAAC ACCTTCAGTC 
ACACCAAACA ATGAGACTGT CACTTCAGGT GAAGACCATA TCAAACGCAG TTTAAGTTCT 
GCGGGCCCCT TTGTAGTGCC TGATCACCTT CGGCAGGATA TAGAAGAATT TGAAGCTCTG 
TATGAACAGC ATAGTAATGA GTATGTTGTC CGTAATAAGA AGCTGTGGGA CATAAACCCA 
AAACAGAAAT GTTCAACTCT ATACGATTAT TTCTCTCAGT TGTTGGAGGA ACATGGTCCC 
TTGGACATGA GTAACAAAAT GTTTTCTGAA GAATATGAGT TCTTCCCAGA AGAAACTCAA 
CAGATACTAG AAAAAGCAGG AGGCTTGAAA GCGTTTCTCC TGGGATGTCC TCGTTTTGTT 
GTGATTGATA ACTGTATCGC ATTGAAGAAA GTTGCATTAC GGCTCAAGAA AAAGAGAAAG 
AAGAAAAACA TTAAAACAAA AGTAGAAGAA ATTTCAAAAA CAGGAGAGTA TTTACGAGTT 
AAACTACCAC TGAATCCAAC TGCTAGGGAA TTTAAACCAG ATGTAAAGTC CAAACCAGTG 
TCTGACTTAC CTTCAGCACC AGCTTCTGAG GACATGAAGC CTCAGCCTGC ACCTGCTAAT 
TCTCCCCAAT CAGCTCGTGA CACTGGGAAG CCAAAACCAG TTTTCGATAA TTCTTCCAGA 
TCAGTTTCTG AGGATGAGAA GCCCAAAGGG ATGTCTTCTG ATTCTCCCAG GCCAGTCTTT 
GAGGATACCG GTCACAAGCC GGTCTCCTCT AATTCTCCCA AACCAGTTCC TGAGGAAGTG 
AAAGCGACCT ATTGGCCTCG ACCCCAGTTG GTCACAGGAT ACTGTACATA TCTTCCCTTT 
CGGGGATTTG ACATTACCCA GACTCCACCA GCCTTCATCA GTGTGTTACC GACTTTGCCC 
CAGTATAGCA TTTACACCCC TCTGGCCAGT GTTCCTACTG AGTATCACCT GCAAAGACCA 
ATACCAGTGG TGCCGTCTTT TGTAGCCAGT GACATAACGG AAGTGCATTT TGAGGCTCAT 
CATTTCAGTG CTGAGAATGC CCCTGGTACC CAGATTGCCT CCGAAACACA GATCCTTGAG 
GAATCTTTGG AAACACCAGT GAAGTCAGAG TGCAGCTATG ATGGTGCTGA TATAGTCCAG 
AGTGAGGCTA GCAGAAACGA TGACTACTGT GGCGATTCTG CCAAATGGGA AGTAAATCCA 
GAAAGCACCA ATGAAGTAAC AAATATTCCA TGTACACAAG TGGTTGCTGT ACAGGTGTCT 
TGGAATATAA CACATCAGGA AGTCAATACT GAGCCATATG GTCCTTTTGA GGCACAACAG 
GGGGGTATTT CACAGGTTGA AAAGGAGTAC CAGGTTTTAC AAGAGCAGCT TAAGGAAGCA 
TGTGAAAATT ATGAACACAG CAATGTCAAG GGCTCAGAAG AAACCAAGGA CTTGGAAGAA 
AAGTTGAAAA GGAATGTTGA AGAAAATAAG ATCTCAAAGA CAGAACTAGA TTGGTTCCTT 
GAAGATTTGA ACAAGGAAAT TAAAAAATGG GAACAGGAGA AAAAAGAAAT CCAAGAAAGA 
CTAAAGGCAC TGAAGAAGAA AATGAAAAAG GTGTTAAATG CCAGCAAAAT GTATACGCAG 
GAAAATGATG GAAAGGAAAA GGCACTTGAA TTACTTCTGG ATCAGTCCCT TGAAATCAGT 
AATACATTTA CAAATGAGAA AATGAAAATA GAAGAGAGTA TAAAGAAAGG GAAAGAGCAT 
TATGAAGAGA GTCTTCAAAG GGCTGTGGAT GCAGAGGTAT CTGTGCTTGA AAACTGGAAG 
GACATGGAAG TGTGTAAGTT ACAGAGCATG GAGTCACAAG CCGAAAGGTT TCTCAAGAAT 
CTGAAGCTAA TGAGCAGTGA TTCGGCAGCG TATTCTGACA TGGACTCAGA CATACATTCA 
TGGGAATCGT TTCTTTCTGA TGTTAGAGAA GAAATTGAGA AAGCAAAGTC TCAGTTTGAA 
GAACAAATTC AGGCAATTAA ATATGGTTCT CAGCTCAGTG AACTTCCTAA AGTGCAGATT 
TCTGAGCTTT CATTTCCTAC CTGTAACACG ATTCATCCTA AATTACTTCC TGAGTCTTCA 
GGCCAGGATG GTCAAGGTCC CTCCACTTCT ACAAGTCATG GGACCGGAGA CCAGACAGCA 
GCTCCCGAGG AGTCAAGTCT GGGCTCTGCA GCTGACAGCC CAGTGGAGTT GCCCTCCTGC 
AGTCAGAAAG CCCCCCAGCT GCCAGAGCAT GAGGGTGCTG GCCAGGCAAC TGGGCCAGAA 
CCAGGCCCAG TGGCTGATCA GAAGTTACCT GCTGCTCCAG AGCGTGCCAC TCATTCAAGC 
CAGTCTCCAA AGAAGCCGTT CAATAGCATT ATTGAGCACC TGTTGGTGGT GTTCCCGTGT 
TACAGCAGCA CGGAGCTTGC TGGTTTTATT AAAAAAGTTA GAAACAAAAA CAAGACCTCT 
CTGTCTGGAT TGAGTATTGA CGAAATTGTG CAAAGAGTGA CAGAACACGT CCTTGATGAA 
GAAAGAAAGA AAAAGCCAAG TCCAGGAAAG GGCTGGAGGC TGTCTGCACC CAGCTCTGCT 
GTTGCTGTGC CCAGGGCTGC CCAGGACCCG CCCTCCATGC CTGTCGGAGC ATCGCCCGAA 
ACCAGGGGGC CGAGGACCGA GGACGTCCCT GTGTCCTGCG CAAATTCCTG TGAAATGTGC 
CGAGAAGTAT TCAAGTCCAA ACACGTGCGT GAGCTGCAGT GTGGGCACAG GCTTCACAAG 
GGGTGCTTCA AGCAGTGGCT GAAGAGCCAG CGCACCTGCC CAGCCTGCCT GGGTCACGAC 
CTCCTGTCAG AGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_005202061.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005202118.1 Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X10, mRNA.

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CloneID	OBa16129
Clone ID Related Accession (Same CDS sequence)	XM_005202061.3 , XM_005202060.1
Accession Version	XM_005202061.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5034bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TTC3 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103826.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005202061.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGGGG AATATGACTG GGCCCTGCAA GCAAACATAA AAGCTCAAAA ACTCTGTAAA 
AATGACCCTG AGGGAATCAA GGATCTAATT CAGCAGCATG TAAAGTTACA AAAACAAATA 
GAAGACCTGC AAGGTCAAAC ACCAGCTAGT AATCCAGTCA AAGCCTTCTG TGAAAGCAGG 
GCCTACATAC CTAGTTTATC AGCACCTGCA TTTAGTACTT CACTTAACTT TGTGGAAACT 
GAAAAAAATT TCAGAAAAAC TAACCACGAA ATGGCAAACG GTGGTAATCA GAATCCAAAG 
GTGGTAGATG AAGCTCTGAA AGTAGATGAT TGTGACTGTC ATCCTGAATT TGTACCGCCA 
TCAAGTCAGC CTCCAAAATA TAAAGGGAAA CAAAAATCTC GAAACAATGA ATTGGAGAAG 
TTCAGCCCTG GTTCACAAGA AACTTTACCA GTAGATTTAA AAAACATCTT GGAGAAACAG 
TTTTCTAAAT CTTCCAGAGC CGCACACCAG GATTTTGCTA ATATAATGAA AATGCTGAGA 
AGTTTAATTC AGGATGGCTA CACGGCCTTG CTGGAGCAGC GCTGCCGCAG CGCCGCCCAA 
GCCTTCACAG AGTTGCTCAA CGGTTTAGAT CCTCAAAAAA TAAAGCAATT GAACCTGGCC 
ATGATTAACT ATGTGTTAGT AGTCTACGGA CTTGCCATTT CACTCCTTGG AATAGGACAG 
CCCGAGGAGT TATCTGAAGC TGAAAACCAG TTCAAGAGGA TGATTGAACA CTACCCCAAT 
GAGGGGCTTG ATTGCTTGGC CTACTGTGGA ATTGGAAAAG TGTATTTGAA AAAAAACAGA 
TTTTTAGAAG CTCTTAATCA TTTTGAGAAA GCAAGAACCT TGATCTGCCG TCTTCCTGGA 
GTGTTAACTT GGCCCACAAG TAATGTGGTT ATTGAGGAGA CTCAGCCAGG GAAGATAAAG 
ATGCTGTTAG AGAAATTTGT TGAAGAATGC AGGTTCCCTC CAGTGCCGGA TGCCACTTGT 
TGCTATCAGA AGTGCCATGG ATATTCCAAA ATCCAGATAT ATATAACTGA TCCAGACTTT 
AAGGGTTTTA TACGGATCAG CTGTTGCCAG TATTGTAAAA TAGAATTTCA CATGAACTGC 
TGGAAGAAGT TAAAAACAAC AACCTTCAAT GATAAAATTG ACAAGGATTT TCTGCAAGGA 
ATCTGTCTTA CTCCTGATTG TGAAGGAATC ATTTCAAAGA TTATCATCTT CAGCAGTGGT 
GGTCAAGTTA AATGTGAATT TGAACACAAG GTCATTAAAG AAAAGGTTCC TCCAAGACCT 
ATTCTGAAAC AGAAATGTTC TAGCCTAGAG AAGTTGAGGC TGAAAGAAGA CAAAAAATTG 
AAGCGAAAGC TCCAAAAAAA GGAAGCAAAA AAATTAGCAC AAGAAAGACT GGAAGAGGAC 
TTAAGGGAAA GTAACCCACC AAAAACTGAA GAGCAGAGAG AAACTATCAA CCATGTCCAA 
AGTTGCCAGT TCCTTGATGA CAGAATTCTG CAGTGCATAA AGCAGTACGC TGACAAGATT 
AAGTCCGGGA TACTGAATAC TTCCAAGCTT CTCAAAGAAT TGCTTTCTTG GAAGGTTTTA 
ACCACAGAAG ACTACACAAC GTGTTTTTCT AGCAGAAATT TCCTGAATGA AGCAGTGGAC 
TATGTCATTC GTCATTTGAT CCAAGAAAAG AATAGAGTAA AGACAAGGGT ATTTCTGCAT 
GTTTTGAGTG AGCTTAAAGA AGTGGACCCA AAACTAGCCA CCTGGATCCA GAAACTGAAT 
AGCTTTGGCT TAGATGCCAC AGGACCTTTC TTTTCTCGTT ATGGGGCTCC TCTTAAGGAG 
CTTGATTTTA GCATCATGAC TTTTCTCTGG AATGAGAAAT ACGGTCATAA GCTAGGCTCT 
ATAGAAGGAA AGCAACTTGA TTATTTCTAT GAGCCTACAT CACTGAAGGA AGCGCGGTGT 
TTAATATGGC TGCTAGAAGA ACACAGAGAC AAGTTCCCAG CACTGCATAA TGCCTTGGAC 
GAGTTCTTTG ATATAATGGA CAGCCGCTGC ACTGTATTAA GGAAACAGGA CAGTGGTGAC 
GTGCCATTTA ATTCTACCAA GATAAAAAAC AAAGGCAAGA AAAAGAAGCC AAAAGATTCA 
AAGCCTATGT TAGTTGGATC TGGAACACCT TCAGTCACAC CAAACAATGA GACTGTCACT 
TCAGGTGAAG ACCATATCAA ACGCAGTTTA AGTTCTGCGG GCCCCTTTGT AGTGCCTGAT 
CACCTTCGGC AGGATATAGA AGAATTTGAA GCTCTGTATG AACAGCATAG TAATGAGTAT 
GTTGTCCGTA ATAAGAAGCT GTGGGACATA AACCCAAAAC AGAAATGTTC AACTCTATAC 
GATTATTTCT CTCAGTTGTT GGAGGAACAT GGTCCCTTGG ACATGAGTAA CAAAATGTTT 
TCTGAAGAAT ATGAGTTCTT CCCAGAAGAA ACTCAACAGA TACTAGAAAA AGCAGGAGGC 
TTGAAAGCGT TTCTCCTGGG ATGTCCTCGT TTTGTTGTGA TTGATAACTG TATCGCATTG 
AAGAAAGTTG CATTACGGCT CAAGAAAAAG AGAAAGAAGA AAAACATTAA AACAAAAGTA 
GAAGAAATTT CAAAAACAGG AGAGTATTTA CGAGTTAAAC TACCACTGAA TCCAACTGCT 
AGGGAATTTA AACCAGATGT AAAGTCCAAA CCAGTGTCTG ACTTACCTTC AGCACCAGCT 
TCTGAGGACA TGAAGCCTCA GCCTGCACCT GCTAATTCTC CCCAATCAGC TCGTGACACT 
GGGAAGCCAA AACCAGTTTT CGATAATTCT TCCAGATCAG TTTCTGAGGA TGAGAAGCCC 
AAAGGGATGT CTTCTGATTC TCCCAGGCCA GTCTTTGAGG ATACCGGTCA CAAGCCGGTC 
TCCTCTAATT CTCCCAAACC AGTTCCTGAG GAAGTGAAAG CGACCTATTG GCCTCGACCC 
CAGTTGGTCA CAGGATACTG TACATATCTT CCCTTTCGGG GATTTGACAT TACCCAGACT 
CCACCAGCCT TCATCAGTGT GTTACCGACT TTGCCCCAGT ATAGCATTTA CACCCCTCTG 
GCCAGTGTTC CTACTGAGTA TCACCTGCAA AGACCAATAC CAGTGGTGCC GTCTTTTGTA 
GCCAGTGACA TAACGGAAGT GCATTTTGAG GCTCATCATT TCAGTGCTGA GAATGCCCCT 
GGTACCCAGA TTGCCTCCGA AACACAGATC CTTGAGGAAT CTTTGGAAAC ACCAGTGAAG 
TCAGAGTGCA GCTATGATGG TGCTGATATA GTCCAGAGTG AGGCTAGCAG AAACGATGAC 
TACTGTGGCG ATTCTGCCAA ATGGGAAGTA AATCCAGAAA GCACCAATGA AGTAACAAAT 
ATTCCATGTA CACAAGTGGT TGCTGTACAG GTGTCTTGGA ATATAACACA TCAGGAAGTC 
AATACTGAGC CATATGGTCC TTTTGAGGCA CAACAGGGGG GTATTTCACA GGTTGAAAAG 
GAGTACCAGG TTTTACAAGA GCAGCTTAAG GAAGCATGTG AAAATTATGA ACACAGCAAT 
GTCAAGGGCT CAGAAGAAAC CAAGGACTTG GAAGAAAAGT TGAAAAGGAA TGTTGAAGAA 
AATAAGATCT CAAAGACAGA ACTAGATTGG TTCCTTGAAG ATTTGAACAA GGAAATTAAA 
AAATGGGAAC AGGAGAAAAA AGAAATCCAA GAAAGACTAA AGGCACTGAA GAAGAAAATG 
AAAAAGGTGT TAAATGCCAG CAAAATGTAT ACGCAGGAAA ATGATGGAAA GGAAAAGGCA 
CTTGAATTAC TTCTGGATCA GTCCCTTGAA ATCAGTAATA CATTTACAAA TGAGAAAATG 
AAAATAGAAG AGAGTATAAA GAAAGGGAAA GAGCATTATG AAGAGAGTCT TCAAAGGGCT 
GTGGATGCAG AGGTATCTGT GCTTGAAAAC TGGAAGGACA TGGAAGTGTG TAAGTTACAG 
AGCATGGAGT CACAAGCCGA AAGGTTTCTC AAGAATCTGA AGCTAATGAG CAGTGATTCG 
GCAGCGTATT CTGACATGGA CTCAGACATA CATTCATGGG AATCGTTTCT TTCTGATGTT 
AGAGAAGAAA TTGAGAAAGC AAAGTCTCAG TTTGAAGAAC AAATTCAGGC AATTAAATAT 
GGTTCTCAGC TCAGTGAACT TCCTAAAGTG CAGATTTCTG AGCTTTCATT TCCTACCTGT 
AACACGATTC ATCCTAAATT ACTTCCTGAG TCTTCAGGCC AGGATGGTCA AGGTCCCTCC 
ACTTCTACAA GTCATGGGAC CGGAGACCAG ACAGCAGCTC CCGAGGAGTC AAGTCTGGGC 
TCTGCAGCTG ACAGCCCAGT GGAGTTGCCC TCCTGCAGTC AGAAAGCCCC CCAGCTGCCA 
GAGCATGAGG GTGCTGGCCA GGCAACTGGG CCAGAACCAG GCCCAGTGGC TGATCAGAAG 
TTACCTGCTG CTCCAGAGCG TGCCACTCAT TCAAGCCAGT CTCCAAAGAA GCCGTTCAAT 
AGCATTATTG AGCACCTGTT GGTGGTGTTC CCGTGTTACA GCAGCACGGA GCTTGCTGGT 
TTTATTAAAA AAGTTAGAAA CAAAAACAAG ACCTCTCTGT CTGGATTGAG TATTGACGAA 
ATTGTGCAAA GAGTGACAGA ACACGTCCTT GATGAAGAAA GAAAGAAAAA GCCAAGTCCA 
GGAAAGGGCT GGAGGCTGTC TGCACCCAGC TCTGCTGTTG CTGTGCCCAG GGCTGCCCAG 
GACCCGCCCT CCATGCCTGT CGGAGCATCG CCCGAAACCA GGGGGCCGAG GACCGAGGAC 
GTCCCTGTGT CCTGCGCAAA TTCCTGTGAA ATGTGCCGAG AAGTATTCAA GTCCAAACAC 
GTGCGTGAGC TGCAGTGTGG GCACAGGCTT CACAAGGGGT GCTTCAAGCA GTGGCTGAAG 
AGCCAGCGCA CCTGCCCAGC CTGCCTGGGT CACGACCTCC TGTCAGAGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005202118.1
CDS	330..5363
Translation	MLGEYDWALQANIKAQKLCKNDPEGIKDLIQQHVKLQKQIEDLQGQTPASNPVKAFCESRAYIPSLSAPAFSTSLNFVETEKNFRKTNHEMANGGNQNPKVVDEALKVDDCDCHPEFVPPSSQPPKYKGKQKSRNNELEKFSPGSQETLPVDLKNILEKQFSKSSRAAHQDFANIMKMLRSLIQDGYTALLEQRCRSAAQAFTELLNGLDPQKIKQLNLAMINYVLVVYGLAISLLGIGQPEELSEAENQFKRMIEHYPNEGLDCLAYCGIGKVYLKKNRFLEALNHFEKARTLICRLPGVLTWPTSNVVIEETQPGKIKMLLEKFVEECRFPPVPDATCCYQKCHGYSKIQIYITDPDFKGFIRISCCQYCKIEFHMNCWKKLKTTTFNDKIDKDFLQGICLTPDCEGIISKIIIFSSGGQVKCEFEHKVIKEKVPPRPILKQKCSSLEKLRLKEDKKLKRKLQKKEAKKLAQERLEEDLRESNPPKTEEQRETINHVQSCQFLDDRILQCIKQYADKIKSGILNTSKLLKELLSWKVLTTEDYTTCFSSRNFLNEAVDYVIRHLIQEKNRVKTRVFLHVLSELKEVDPKLATWIQKLNSFGLDATGPFFSRYGAPLKELDFSIMTFLWNEKYGHKLGSIEGKQLDYFYEPTSLKEARCLIWLLEEHRDKFPALHNALDEFFDIMDSRCTVLRKQDSGDVPFNSTKIKNKGKKKKPKDSKPMLVGSGTPSVTPNNETVTSGEDHIKRSLSSAGPFVVPDHLRQDIEEFEALYEQHSNEYVVRNKKLWDINPKQKCSTLYDYFSQLLEEHGPLDMSNKMFSEEYEFFPEETQQILEKAGGLKAFLLGCPRFVVIDNCIALKKVALRLKKKRKKKNIKTKVEEISKTGEYLRVKLPLNPTAREFKPDVKSKPVSDLPSAPASEDMKPQPAPANSPQSARDTGKPKPVFDNSSRSVSEDEKPKGMSSDSPRPVFEDTGHKPVSSNSPKPVPEEVKATYWPRPQLVTGYCTYLPFRGFDITQTPPAFISVLPTLPQYSIYTPLASVPTEYHLQRPIPVVPSFVASDITEVHFEAHHFSAENAPGTQIASETQILEESLETPVKSECSYDGADIVQSEASRNDDYCGDSAKWEVNPESTNEVTNIPCTQVVAVQVSWNITHQEVNTEPYGPFEAQQGGISQVEKEYQVLQEQLKEACENYEHSNVKGSEETKDLEEKLKRNVEENKISKTELDWFLEDLNKEIKKWEQEKKEIQERLKALKKKMKKVLNASKMYTQENDGKEKALELLLDQSLEISNTFTNEKMKIEESIKKGKEHYEESLQRAVDAEVSVLENWKDMEVCKLQSMESQAERFLKNLKLMSSDSAAYSDMDSDIHSWESFLSDVREEIEKAKSQFEEQIQAIKYGSQLSELPKVQISELSFPTCNTIHPKLLPESSGQDGQGPSTSTSHGTGDQTAAPEESSLGSAADSPVELPSCSQKAPQLPEHEGAGQATGPEPGPVADQKLPAAPERATHSSQSPKKPFNSIIEHLLVVFPCYSSTELAGFIKKVRNKNKTSLSGLSIDEIVQRVTEHVLDEERKKKPSPGKGWRLSAPSSAVAVPRAAQDPPSMPVGASPETRGPRTEDVPVSCANSCEMCREVFKSKHVRELQCGHRLHKGCFKQWLKSQRTCPACLGHDLLSEE

Target ORF information:

RefSeq Version	XM_005202061.3
Organism	Bos taurus(cattle)
Definition	Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005202061.3

ORF Insert Sequence:

ATGCTGGGGG AATATGACTG GGCCCTGCAA GCAAACATAA AAGCTCAAAA ACTCTGTAAA 
AATGACCCTG AGGGAATCAA GGATCTAATT CAGCAGCATG TAAAGTTACA AAAACAAATA 
GAAGACCTGC AAGGTCAAAC ACCAGCTAGT AATCCAGTCA AAGCCTTCTG TGAAAGCAGG 
GCCTACATAC CTAGTTTATC AGCACCTGCA TTTAGTACTT CACTTAACTT TGTGGAAACT 
GAAAAAAATT TCAGAAAAAC TAACCACGAA ATGGCAAACG GTGGTAATCA GAATCCAAAG 
GTGGTAGATG AAGCTCTGAA AGTAGATGAT TGTGACTGTC ATCCTGAATT TGTACCGCCA 
TCAAGTCAGC CTCCAAAATA TAAAGGGAAA CAAAAATCTC GAAACAATGA ATTGGAGAAG 
TTCAGCCCTG GTTCACAAGA AACTTTACCA GTAGATTTAA AAAACATCTT GGAGAAACAG 
TTTTCTAAAT CTTCCAGAGC CGCACACCAG GATTTTGCTA ATATAATGAA AATGCTGAGA 
AGTTTAATTC AGGATGGCTA CACGGCCTTG CTGGAGCAGC GCTGCCGCAG CGCCGCCCAA 
GCCTTCACAG AGTTGCTCAA CGGTTTAGAT CCTCAAAAAA TAAAGCAATT GAACCTGGCC 
ATGATTAACT ATGTGTTAGT AGTCTACGGA CTTGCCATTT CACTCCTTGG AATAGGACAG 
CCCGAGGAGT TATCTGAAGC TGAAAACCAG TTCAAGAGGA TGATTGAACA CTACCCCAAT 
GAGGGGCTTG ATTGCTTGGC CTACTGTGGA ATTGGAAAAG TGTATTTGAA AAAAAACAGA 
TTTTTAGAAG CTCTTAATCA TTTTGAGAAA GCAAGAACCT TGATCTGCCG TCTTCCTGGA 
GTGTTAACTT GGCCCACAAG TAATGTGGTT ATTGAGGAGA CTCAGCCAGG GAAGATAAAG 
ATGCTGTTAG AGAAATTTGT TGAAGAATGC AGGTTCCCTC CAGTGCCGGA TGCCACTTGT 
TGCTATCAGA AGTGCCATGG ATATTCCAAA ATCCAGATAT ATATAACTGA TCCAGACTTT 
AAGGGTTTTA TACGGATCAG CTGTTGCCAG TATTGTAAAA TAGAATTTCA CATGAACTGC 
TGGAAGAAGT TAAAAACAAC AACCTTCAAT GATAAAATTG ACAAGGATTT TCTGCAAGGA 
ATCTGTCTTA CTCCTGATTG TGAAGGAATC ATTTCAAAGA TTATCATCTT CAGCAGTGGT 
GGTCAAGTTA AATGTGAATT TGAACACAAG GTCATTAAAG AAAAGGTTCC TCCAAGACCT 
ATTCTGAAAC AGAAATGTTC TAGCCTAGAG AAGTTGAGGC TGAAAGAAGA CAAAAAATTG 
AAGCGAAAGC TCCAAAAAAA GGAAGCAAAA AAATTAGCAC AAGAAAGACT GGAAGAGGAC 
TTAAGGGAAA GTAACCCACC AAAAACTGAA GAGCAGAGAG AAACTATCAA CCATGTCCAA 
AGTTGCCAGT TCCTTGATGA CAGAATTCTG CAGTGCATAA AGCAGTACGC TGACAAGATT 
AAGTCCGGGA TACTGAATAC TTCCAAGCTT CTCAAAGAAT TGCTTTCTTG GAAGGTTTTA 
ACCACAGAAG ACTACACAAC GTGTTTTTCT AGCAGAAATT TCCTGAATGA AGCAGTGGAC 
TATGTCATTC GTCATTTGAT CCAAGAAAAG AATAGAGTAA AGACAAGGGT ATTTCTGCAT 
GTTTTGAGTG AGCTTAAAGA AGTGGACCCA AAACTAGCCA CCTGGATCCA GAAACTGAAT 
AGCTTTGGCT TAGATGCCAC AGGACCTTTC TTTTCTCGTT ATGGGGCTCC TCTTAAGGAG 
CTTGATTTTA GCATCATGAC TTTTCTCTGG AATGAGAAAT ACGGTCATAA GCTAGGCTCT 
ATAGAAGGAA AGCAACTTGA TTATTTCTAT GAGCCTACAT CACTGAAGGA AGCGCGGTGT 
TTAATATGGC TGCTAGAAGA ACACAGAGAC AAGTTCCCAG CACTGCATAA TGCCTTGGAC 
GAGTTCTTTG ATATAATGGA CAGCCGCTGC ACTGTATTAA GGAAACAGGA CAGTGGTGAC 
GTGCCATTTA ATTCTACCAA GATAAAAAAC AAAGGCAAGA AAAAGAAGCC AAAAGATTCA 
AAGCCTATGT TAGTTGGATC TGGAACACCT TCAGTCACAC CAAACAATGA GACTGTCACT 
TCAGGTGAAG ACCATATCAA ACGCAGTTTA AGTTCTGCGG GCCCCTTTGT AGTGCCTGAT 
CACCTTCGGC AGGATATAGA AGAATTTGAA GCTCTGTATG AACAGCATAG TAATGAGTAT 
GTTGTCCGTA ATAAGAAGCT GTGGGACATA AACCCAAAAC AGAAATGTTC AACTCTATAC 
GATTATTTCT CTCAGTTGTT GGAGGAACAT GGTCCCTTGG ACATGAGTAA CAAAATGTTT 
TCTGAAGAAT ATGAGTTCTT CCCAGAAGAA ACTCAACAGA TACTAGAAAA AGCAGGAGGC 
TTGAAAGCGT TTCTCCTGGG ATGTCCTCGT TTTGTTGTGA TTGATAACTG TATCGCATTG 
AAGAAAGTTG CATTACGGCT CAAGAAAAAG AGAAAGAAGA AAAACATTAA AACAAAAGTA 
GAAGAAATTT CAAAAACAGG AGAGTATTTA CGAGTTAAAC TACCACTGAA TCCAACTGCT 
AGGGAATTTA AACCAGATGT AAAGTCCAAA CCAGTGTCTG ACTTACCTTC AGCACCAGCT 
TCTGAGGACA TGAAGCCTCA GCCTGCACCT GCTAATTCTC CCCAATCAGC TCGTGACACT 
GGGAAGCCAA AACCAGTTTT CGATAATTCT TCCAGATCAG TTTCTGAGGA TGAGAAGCCC 
AAAGGGATGT CTTCTGATTC TCCCAGGCCA GTCTTTGAGG ATACCGGTCA CAAGCCGGTC 
TCCTCTAATT CTCCCAAACC AGTTCCTGAG GAAGTGAAAG CGACCTATTG GCCTCGACCC 
CAGTTGGTCA CAGGATACTG TACATATCTT CCCTTTCGGG GATTTGACAT TACCCAGACT 
CCACCAGCCT TCATCAGTGT GTTACCGACT TTGCCCCAGT ATAGCATTTA CACCCCTCTG 
GCCAGTGTTC CTACTGAGTA TCACCTGCAA AGACCAATAC CAGTGGTGCC GTCTTTTGTA 
GCCAGTGACA TAACGGAAGT GCATTTTGAG GCTCATCATT TCAGTGCTGA GAATGCCCCT 
GGTACCCAGA TTGCCTCCGA AACACAGATC CTTGAGGAAT CTTTGGAAAC ACCAGTGAAG 
TCAGAGTGCA GCTATGATGG TGCTGATATA GTCCAGAGTG AGGCTAGCAG AAACGATGAC 
TACTGTGGCG ATTCTGCCAA ATGGGAAGTA AATCCAGAAA GCACCAATGA AGTAACAAAT 
ATTCCATGTA CACAAGTGGT TGCTGTACAG GTGTCTTGGA ATATAACACA TCAGGAAGTC 
AATACTGAGC CATATGGTCC TTTTGAGGCA CAACAGGGGG GTATTTCACA GGTTGAAAAG 
GAGTACCAGG TTTTACAAGA GCAGCTTAAG GAAGCATGTG AAAATTATGA ACACAGCAAT 
GTCAAGGGCT CAGAAGAAAC CAAGGACTTG GAAGAAAAGT TGAAAAGGAA TGTTGAAGAA 
AATAAGATCT CAAAGACAGA ACTAGATTGG TTCCTTGAAG ATTTGAACAA GGAAATTAAA 
AAATGGGAAC AGGAGAAAAA AGAAATCCAA GAAAGACTAA AGGCACTGAA GAAGAAAATG 
AAAAAGGTGT TAAATGCCAG CAAAATGTAT ACGCAGGAAA ATGATGGAAA GGAAAAGGCA 
CTTGAATTAC TTCTGGATCA GTCCCTTGAA ATCAGTAATA CATTTACAAA TGAGAAAATG 
AAAATAGAAG AGAGTATAAA GAAAGGGAAA GAGCATTATG AAGAGAGTCT TCAAAGGGCT 
GTGGATGCAG AGGTATCTGT GCTTGAAAAC TGGAAGGACA TGGAAGTGTG TAAGTTACAG 
AGCATGGAGT CACAAGCCGA AAGGTTTCTC AAGAATCTGA AGCTAATGAG CAGTGATTCG 
GCAGCGTATT CTGACATGGA CTCAGACATA CATTCATGGG AATCGTTTCT TTCTGATGTT 
AGAGAAGAAA TTGAGAAAGC AAAGTCTCAG TTTGAAGAAC AAATTCAGGC AATTAAATAT 
GGTTCTCAGC TCAGTGAACT TCCTAAAGTG CAGATTTCTG AGCTTTCATT TCCTACCTGT 
AACACGATTC ATCCTAAATT ACTTCCTGAG TCTTCAGGCC AGGATGGTCA AGGTCCCTCC 
ACTTCTACAA GTCATGGGAC CGGAGACCAG ACAGCAGCTC CCGAGGAGTC AAGTCTGGGC 
TCTGCAGCTG ACAGCCCAGT GGAGTTGCCC TCCTGCAGTC AGAAAGCCCC CCAGCTGCCA 
GAGCATGAGG GTGCTGGCCA GGCAACTGGG CCAGAACCAG GCCCAGTGGC TGATCAGAAG 
TTACCTGCTG CTCCAGAGCG TGCCACTCAT TCAAGCCAGT CTCCAAAGAA GCCGTTCAAT 
AGCATTATTG AGCACCTGTT GGTGGTGTTC CCGTGTTACA GCAGCACGGA GCTTGCTGGT 
TTTATTAAAA AAGTTAGAAA CAAAAACAAG ACCTCTCTGT CTGGATTGAG TATTGACGAA 
ATTGTGCAAA GAGTGACAGA ACACGTCCTT GATGAAGAAA GAAAGAAAAA GCCAAGTCCA 
GGAAAGGGCT GGAGGCTGTC TGCACCCAGC TCTGCTGTTG CTGTGCCCAG GGCTGCCCAG 
GACCCGCCCT CCATGCCTGT CGGAGCATCG CCCGAAACCA GGGGGCCGAG GACCGAGGAC 
GTCCCTGTGT CCTGCGCAAA TTCCTGTGAA ATGTGCCGAG AAGTATTCAA GTCCAAACAC 
GTGCGTGAGC TGCAGTGTGG GCACAGGCTT CACAAGGGGT GCTTCAAGCA GTGGCTGAAG 
AGCCAGCGCA CCTGCCCAGC CTGCCTGGGT CACGACCTCC TGTCAGAGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_005202060.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005202117.1 Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X9, mRNA.

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CloneID	OBa16129
Clone ID Related Accession (Same CDS sequence)	XM_005202061.3 , XM_005202060.1
Accession Version	XM_005202060.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5034bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TTC3 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103826.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGGGG AATATGACTG GGCCCTGCAA GCAAACATAA AAGCTCAAAA ACTCTGTAAA 
AATGACCCTG AGGGAATCAA GGATCTAATT CAGCAGCATG TAAAGTTACA AAAACAAATA 
GAAGACCTGC AAGGTCAAAC ACCAGCTAGT AATCCAGTCA AAGCCTTCTG TGAAAGCAGG 
GCCTACATAC CTAGTTTATC AGCACCTGCA TTTAGTACTT CACTTAACTT TGTGGAAACT 
GAAAAAAATT TCAGAAAAAC TAACCACGAA ATGGCAAACG GTGGTAATCA GAATCCAAAG 
GTGGTAGATG AAGCTCTGAA AGTAGATGAT TGTGACTGTC ATCCTGAATT TGTACCGCCA 
TCAAGTCAGC CTCCAAAATA TAAAGGGAAA CAAAAATCTC GAAACAATGA ATTGGAGAAG 
TTCAGCCCTG GTTCACAAGA AACTTTACCA GTAGATTTAA AAAACATCTT GGAGAAACAG 
TTTTCTAAAT CTTCCAGAGC CGCACACCAG GATTTTGCTA ATATAATGAA AATGCTGAGA 
AGTTTAATTC AGGATGGCTA CACGGCCTTG CTGGAGCAGC GCTGCCGCAG CGCCGCCCAA 
GCCTTCACAG AGTTGCTCAA CGGTTTAGAT CCTCAAAAAA TAAAGCAATT GAACCTGGCC 
ATGATTAACT ATGTGTTAGT AGTCTACGGA CTTGCCATTT CACTCCTTGG AATAGGACAG 
CCCGAGGAGT TATCTGAAGC TGAAAACCAG TTCAAGAGGA TGATTGAACA CTACCCCAAT 
GAGGGGCTTG ATTGCTTGGC CTACTGTGGA ATTGGAAAAG TGTATTTGAA AAAAAACAGA 
TTTTTAGAAG CTCTTAATCA TTTTGAGAAA GCAAGAACCT TGATCTGCCG TCTTCCTGGA 
GTGTTAACTT GGCCCACAAG TAATGTGGTT ATTGAGGAGA CTCAGCCAGG GAAGATAAAG 
ATGCTGTTAG AGAAATTTGT TGAAGAATGC AGGTTCCCTC CAGTGCCGGA TGCCACTTGT 
TGCTATCAGA AGTGCCATGG ATATTCCAAA ATCCAGATAT ATATAACTGA TCCAGACTTT 
AAGGGTTTTA TACGGATCAG CTGTTGCCAG TATTGTAAAA TAGAATTTCA CATGAACTGC 
TGGAAGAAGT TAAAAACAAC AACCTTCAAT GATAAAATTG ACAAGGATTT TCTGCAAGGA 
ATCTGTCTTA CTCCTGATTG TGAAGGAATC ATTTCAAAGA TTATCATCTT CAGCAGTGGT 
GGTCAAGTTA AATGTGAATT TGAACACAAG GTCATTAAAG AAAAGGTTCC TCCAAGACCT 
ATTCTGAAAC AGAAATGTTC TAGCCTAGAG AAGTTGAGGC TGAAAGAAGA CAAAAAATTG 
AAGCGAAAGC TCCAAAAAAA GGAAGCAAAA AAATTAGCAC AAGAAAGACT GGAAGAGGAC 
TTAAGGGAAA GTAACCCACC AAAAACTGAA GAGCAGAGAG AAACTATCAA CCATGTCCAA 
AGTTGCCAGT TCCTTGATGA CAGAATTCTG CAGTGCATAA AGCAGTACGC TGACAAGATT 
AAGTCCGGGA TACTGAATAC TTCCAAGCTT CTCAAAGAAT TGCTTTCTTG GAAGGTTTTA 
ACCACAGAAG ACTACACAAC GTGTTTTTCT AGCAGAAATT TCCTGAATGA AGCAGTGGAC 
TATGTCATTC GTCATTTGAT CCAAGAAAAG AATAGAGTAA AGACAAGGGT ATTTCTGCAT 
GTTTTGAGTG AGCTTAAAGA AGTGGACCCA AAACTAGCCA CCTGGATCCA GAAACTGAAT 
AGCTTTGGCT TAGATGCCAC AGGACCTTTC TTTTCTCGTT ATGGGGCTCC TCTTAAGGAG 
CTTGATTTTA GCATCATGAC TTTTCTCTGG AATGAGAAAT ACGGTCATAA GCTAGGCTCT 
ATAGAAGGAA AGCAACTTGA TTATTTCTAT GAGCCTACAT CACTGAAGGA AGCGCGGTGT 
TTAATATGGC TGCTAGAAGA ACACAGAGAC AAGTTCCCAG CACTGCATAA TGCCTTGGAC 
GAGTTCTTTG ATATAATGGA CAGCCGCTGC ACTGTATTAA GGAAACAGGA CAGTGGTGAC 
GTGCCATTTA ATTCTACCAA GATAAAAAAC AAAGGCAAGA AAAAGAAGCC AAAAGATTCA 
AAGCCTATGT TAGTTGGATC TGGAACACCT TCAGTCACAC CAAACAATGA GACTGTCACT 
TCAGGTGAAG ACCATATCAA ACGCAGTTTA AGTTCTGCGG GCCCCTTTGT AGTGCCTGAT 
CACCTTCGGC AGGATATAGA AGAATTTGAA GCTCTGTATG AACAGCATAG TAATGAGTAT 
GTTGTCCGTA ATAAGAAGCT GTGGGACATA AACCCAAAAC AGAAATGTTC AACTCTATAC 
GATTATTTCT CTCAGTTGTT GGAGGAACAT GGTCCCTTGG ACATGAGTAA CAAAATGTTT 
TCTGAAGAAT ATGAGTTCTT CCCAGAAGAA ACTCAACAGA TACTAGAAAA AGCAGGAGGC 
TTGAAAGCGT TTCTCCTGGG ATGTCCTCGT TTTGTTGTGA TTGATAACTG TATCGCATTG 
AAGAAAGTTG CATTACGGCT CAAGAAAAAG AGAAAGAAGA AAAACATTAA AACAAAAGTA 
GAAGAAATTT CAAAAACAGG AGAGTATTTA CGAGTTAAAC TACCACTGAA TCCAACTGCT 
AGGGAATTTA AACCAGATGT AAAGTCCAAA CCAGTGTCTG ACTTACCTTC AGCACCAGCT 
TCTGAGGACA TGAAGCCTCA GCCTGCACCT GCTAATTCTC CCCAATCAGC TCGTGACACT 
GGGAAGCCAA AACCAGTTTT CGATAATTCT TCCAGATCAG TTTCTGAGGA TGAGAAGCCC 
AAAGGGATGT CTTCTGATTC TCCCAGGCCA GTCTTTGAGG ATACCGGTCA CAAGCCGGTC 
TCCTCTAATT CTCCCAAACC AGTTCCTGAG GAAGTGAAAG CGACCTATTG GCCTCGACCC 
CAGTTGGTCA CAGGATACTG TACATATCTT CCCTTTCGGG GATTTGACAT TACCCAGACT 
CCACCAGCCT TCATCAGTGT GTTACCGACT TTGCCCCAGT ATAGCATTTA CACCCCTCTG 
GCCAGTGTTC CTACTGAGTA TCACCTGCAA AGACCAATAC CAGTGGTGCC GTCTTTTGTA 
GCCAGTGACA TAACGGAAGT GCATTTTGAG GCTCATCATT TCAGTGCTGA GAATGCCCCT 
GGTACCCAGA TTGCCTCCGA AACACAGATC CTTGAGGAAT CTTTGGAAAC ACCAGTGAAG 
TCAGAGTGCA GCTATGATGG TGCTGATATA GTCCAGAGTG AGGCTAGCAG AAACGATGAC 
TACTGTGGCG ATTCTGCCAA ATGGGAAGTA AATCCAGAAA GCACCAATGA AGTAACAAAT 
ATTCCATGTA CACAAGTGGT TGCTGTACAG GTGTCTTGGA ATATAACACA TCAGGAAGTC 
AATACTGAGC CATATGGTCC TTTTGAGGCA CAACAGGGGG GTATTTCACA GGTTGAAAAG 
GAGTACCAGG TTTTACAAGA GCAGCTTAAG GAAGCATGTG AAAATTATGA ACACAGCAAT 
GTCAAGGGCT CAGAAGAAAC CAAGGACTTG GAAGAAAAGT TGAAAAGGAA TGTTGAAGAA 
AATAAGATCT CAAAGACAGA ACTAGATTGG TTCCTTGAAG ATTTGAACAA GGAAATTAAA 
AAATGGGAAC AGGAGAAAAA AGAAATCCAA GAAAGACTAA AGGCACTGAA GAAGAAAATG 
AAAAAGGTGT TAAATGCCAG CAAAATGTAT ACGCAGGAAA ATGATGGAAA GGAAAAGGCA 
CTTGAATTAC TTCTGGATCA GTCCCTTGAA ATCAGTAATA CATTTACAAA TGAGAAAATG 
AAAATAGAAG AGAGTATAAA GAAAGGGAAA GAGCATTATG AAGAGAGTCT TCAAAGGGCT 
GTGGATGCAG AGGTATCTGT GCTTGAAAAC TGGAAGGACA TGGAAGTGTG TAAGTTACAG 
AGCATGGAGT CACAAGCCGA AAGGTTTCTC AAGAATCTGA AGCTAATGAG CAGTGATTCG 
GCAGCGTATT CTGACATGGA CTCAGACATA CATTCATGGG AATCGTTTCT TTCTGATGTT 
AGAGAAGAAA TTGAGAAAGC AAAGTCTCAG TTTGAAGAAC AAATTCAGGC AATTAAATAT 
GGTTCTCAGC TCAGTGAACT TCCTAAAGTG CAGATTTCTG AGCTTTCATT TCCTACCTGT 
AACACGATTC ATCCTAAATT ACTTCCTGAG TCTTCAGGCC AGGATGGTCA AGGTCCCTCC 
ACTTCTACAA GTCATGGGAC CGGAGACCAG ACAGCAGCTC CCGAGGAGTC AAGTCTGGGC 
TCTGCAGCTG ACAGCCCAGT GGAGTTGCCC TCCTGCAGTC AGAAAGCCCC CCAGCTGCCA 
GAGCATGAGG GTGCTGGCCA GGCAACTGGG CCAGAACCAG GCCCAGTGGC TGATCAGAAG 
TTACCTGCTG CTCCAGAGCG TGCCACTCAT TCAAGCCAGT CTCCAAAGAA GCCGTTCAAT 
AGCATTATTG AGCACCTGTT GGTGGTGTTC CCGTGTTACA GCAGCACGGA GCTTGCTGGT 
TTTATTAAAA AAGTTAGAAA CAAAAACAAG ACCTCTCTGT CTGGATTGAG TATTGACGAA 
ATTGTGCAAA GAGTGACAGA ACACGTCCTT GATGAAGAAA GAAAGAAAAA GCCAAGTCCA 
GGAAAGGGCT GGAGGCTGTC TGCACCCAGC TCTGCTGTTG CTGTGCCCAG GGCTGCCCAG 
GACCCGCCCT CCATGCCTGT CGGAGCATCG CCCGAAACCA GGGGGCCGAG GACCGAGGAC 
GTCCCTGTGT CCTGCGCAAA TTCCTGTGAA ATGTGCCGAG AAGTATTCAA GTCCAAACAC 
GTGCGTGAGC TGCAGTGTGG GCACAGGCTT CACAAGGGGT GCTTCAAGCA GTGGCTGAAG 
AGCCAGCGCA CCTGCCCAGC CTGCCTGGGT CACGACCTCC TGTCAGAGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005202117.1
CDS	198..5231
Translation	MLGEYDWALQANIKAQKLCKNDPEGIKDLIQQHVKLQKQIEDLQGQTPASNPVKAFCESRAYIPSLSAPAFSTSLNFVETEKNFRKTNHEMANGGNQNPKVVDEALKVDDCDCHPEFVPPSSQPPKYKGKQKSRNNELEKFSPGSQETLPVDLKNILEKQFSKSSRAAHQDFANIMKMLRSLIQDGYTALLEQRCRSAAQAFTELLNGLDPQKIKQLNLAMINYVLVVYGLAISLLGIGQPEELSEAENQFKRMIEHYPNEGLDCLAYCGIGKVYLKKNRFLEALNHFEKARTLICRLPGVLTWPTSNVVIEETQPGKIKMLLEKFVEECRFPPVPDATCCYQKCHGYSKIQIYITDPDFKGFIRISCCQYCKIEFHMNCWKKLKTTTFNDKIDKDFLQGICLTPDCEGIISKIIIFSSGGQVKCEFEHKVIKEKVPPRPILKQKCSSLEKLRLKEDKKLKRKLQKKEAKKLAQERLEEDLRESNPPKTEEQRETINHVQSCQFLDDRILQCIKQYADKIKSGILNTSKLLKELLSWKVLTTEDYTTCFSSRNFLNEAVDYVIRHLIQEKNRVKTRVFLHVLSELKEVDPKLATWIQKLNSFGLDATGPFFSRYGAPLKELDFSIMTFLWNEKYGHKLGSIEGKQLDYFYEPTSLKEARCLIWLLEEHRDKFPALHNALDEFFDIMDSRCTVLRKQDSGDVPFNSTKIKNKGKKKKPKDSKPMLVGSGTPSVTPNNETVTSGEDHIKRSLSSAGPFVVPDHLRQDIEEFEALYEQHSNEYVVRNKKLWDINPKQKCSTLYDYFSQLLEEHGPLDMSNKMFSEEYEFFPEETQQILEKAGGLKAFLLGCPRFVVIDNCIALKKVALRLKKKRKKKNIKTKVEEISKTGEYLRVKLPLNPTAREFKPDVKSKPVSDLPSAPASEDMKPQPAPANSPQSARDTGKPKPVFDNSSRSVSEDEKPKGMSSDSPRPVFEDTGHKPVSSNSPKPVPEEVKATYWPRPQLVTGYCTYLPFRGFDITQTPPAFISVLPTLPQYSIYTPLASVPTEYHLQRPIPVVPSFVASDITEVHFEAHHFSAENAPGTQIASETQILEESLETPVKSECSYDGADIVQSEASRNDDYCGDSAKWEVNPESTNEVTNIPCTQVVAVQVSWNITHQEVNTEPYGPFEAQQGGISQVEKEYQVLQEQLKEACENYEHSNVKGSEETKDLEEKLKRNVEENKISKTELDWFLEDLNKEIKKWEQEKKEIQERLKALKKKMKKVLNASKMYTQENDGKEKALELLLDQSLEISNTFTNEKMKIEESIKKGKEHYEESLQRAVDAEVSVLENWKDMEVCKLQSMESQAERFLKNLKLMSSDSAAYSDMDSDIHSWESFLSDVREEIEKAKSQFEEQIQAIKYGSQLSELPKVQISELSFPTCNTIHPKLLPESSGQDGQGPSTSTSHGTGDQTAAPEESSLGSAADSPVELPSCSQKAPQLPEHEGAGQATGPEPGPVADQKLPAAPERATHSSQSPKKPFNSIIEHLLVVFPCYSSTELAGFIKKVRNKNKTSLSGLSIDEIVQRVTEHVLDEERKKKPSPGKGWRLSAPSSAVAVPRAAQDPPSMPVGASPETRGPRTEDVPVSCANSCEMCREVFKSKHVRELQCGHRLHKGCFKQWLKSQRTCPACLGHDLLSEE

Target ORF information:

RefSeq Version	XM_005202060.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005202060.1

ORF Insert Sequence:

ATGCTGGGGG AATATGACTG GGCCCTGCAA GCAAACATAA AAGCTCAAAA ACTCTGTAAA 
AATGACCCTG AGGGAATCAA GGATCTAATT CAGCAGCATG TAAAGTTACA AAAACAAATA 
GAAGACCTGC AAGGTCAAAC ACCAGCTAGT AATCCAGTCA AAGCCTTCTG TGAAAGCAGG 
GCCTACATAC CTAGTTTATC AGCACCTGCA TTTAGTACTT CACTTAACTT TGTGGAAACT 
GAAAAAAATT TCAGAAAAAC TAACCACGAA ATGGCAAACG GTGGTAATCA GAATCCAAAG 
GTGGTAGATG AAGCTCTGAA AGTAGATGAT TGTGACTGTC ATCCTGAATT TGTACCGCCA 
TCAAGTCAGC CTCCAAAATA TAAAGGGAAA CAAAAATCTC GAAACAATGA ATTGGAGAAG 
TTCAGCCCTG GTTCACAAGA AACTTTACCA GTAGATTTAA AAAACATCTT GGAGAAACAG 
TTTTCTAAAT CTTCCAGAGC CGCACACCAG GATTTTGCTA ATATAATGAA AATGCTGAGA 
AGTTTAATTC AGGATGGCTA CACGGCCTTG CTGGAGCAGC GCTGCCGCAG CGCCGCCCAA 
GCCTTCACAG AGTTGCTCAA CGGTTTAGAT CCTCAAAAAA TAAAGCAATT GAACCTGGCC 
ATGATTAACT ATGTGTTAGT AGTCTACGGA CTTGCCATTT CACTCCTTGG AATAGGACAG 
CCCGAGGAGT TATCTGAAGC TGAAAACCAG TTCAAGAGGA TGATTGAACA CTACCCCAAT 
GAGGGGCTTG ATTGCTTGGC CTACTGTGGA ATTGGAAAAG TGTATTTGAA AAAAAACAGA 
TTTTTAGAAG CTCTTAATCA TTTTGAGAAA GCAAGAACCT TGATCTGCCG TCTTCCTGGA 
GTGTTAACTT GGCCCACAAG TAATGTGGTT ATTGAGGAGA CTCAGCCAGG GAAGATAAAG 
ATGCTGTTAG AGAAATTTGT TGAAGAATGC AGGTTCCCTC CAGTGCCGGA TGCCACTTGT 
TGCTATCAGA AGTGCCATGG ATATTCCAAA ATCCAGATAT ATATAACTGA TCCAGACTTT 
AAGGGTTTTA TACGGATCAG CTGTTGCCAG TATTGTAAAA TAGAATTTCA CATGAACTGC 
TGGAAGAAGT TAAAAACAAC AACCTTCAAT GATAAAATTG ACAAGGATTT TCTGCAAGGA 
ATCTGTCTTA CTCCTGATTG TGAAGGAATC ATTTCAAAGA TTATCATCTT CAGCAGTGGT 
GGTCAAGTTA AATGTGAATT TGAACACAAG GTCATTAAAG AAAAGGTTCC TCCAAGACCT 
ATTCTGAAAC AGAAATGTTC TAGCCTAGAG AAGTTGAGGC TGAAAGAAGA CAAAAAATTG 
AAGCGAAAGC TCCAAAAAAA GGAAGCAAAA AAATTAGCAC AAGAAAGACT GGAAGAGGAC 
TTAAGGGAAA GTAACCCACC AAAAACTGAA GAGCAGAGAG AAACTATCAA CCATGTCCAA 
AGTTGCCAGT TCCTTGATGA CAGAATTCTG CAGTGCATAA AGCAGTACGC TGACAAGATT 
AAGTCCGGGA TACTGAATAC TTCCAAGCTT CTCAAAGAAT TGCTTTCTTG GAAGGTTTTA 
ACCACAGAAG ACTACACAAC GTGTTTTTCT AGCAGAAATT TCCTGAATGA AGCAGTGGAC 
TATGTCATTC GTCATTTGAT CCAAGAAAAG AATAGAGTAA AGACAAGGGT ATTTCTGCAT 
GTTTTGAGTG AGCTTAAAGA AGTGGACCCA AAACTAGCCA CCTGGATCCA GAAACTGAAT 
AGCTTTGGCT TAGATGCCAC AGGACCTTTC TTTTCTCGTT ATGGGGCTCC TCTTAAGGAG 
CTTGATTTTA GCATCATGAC TTTTCTCTGG AATGAGAAAT ACGGTCATAA GCTAGGCTCT 
ATAGAAGGAA AGCAACTTGA TTATTTCTAT GAGCCTACAT CACTGAAGGA AGCGCGGTGT 
TTAATATGGC TGCTAGAAGA ACACAGAGAC AAGTTCCCAG CACTGCATAA TGCCTTGGAC 
GAGTTCTTTG ATATAATGGA CAGCCGCTGC ACTGTATTAA GGAAACAGGA CAGTGGTGAC 
GTGCCATTTA ATTCTACCAA GATAAAAAAC AAAGGCAAGA AAAAGAAGCC AAAAGATTCA 
AAGCCTATGT TAGTTGGATC TGGAACACCT TCAGTCACAC CAAACAATGA GACTGTCACT 
TCAGGTGAAG ACCATATCAA ACGCAGTTTA AGTTCTGCGG GCCCCTTTGT AGTGCCTGAT 
CACCTTCGGC AGGATATAGA AGAATTTGAA GCTCTGTATG AACAGCATAG TAATGAGTAT 
GTTGTCCGTA ATAAGAAGCT GTGGGACATA AACCCAAAAC AGAAATGTTC AACTCTATAC 
GATTATTTCT CTCAGTTGTT GGAGGAACAT GGTCCCTTGG ACATGAGTAA CAAAATGTTT 
TCTGAAGAAT ATGAGTTCTT CCCAGAAGAA ACTCAACAGA TACTAGAAAA AGCAGGAGGC 
TTGAAAGCGT TTCTCCTGGG ATGTCCTCGT TTTGTTGTGA TTGATAACTG TATCGCATTG 
AAGAAAGTTG CATTACGGCT CAAGAAAAAG AGAAAGAAGA AAAACATTAA AACAAAAGTA 
GAAGAAATTT CAAAAACAGG AGAGTATTTA CGAGTTAAAC TACCACTGAA TCCAACTGCT 
AGGGAATTTA AACCAGATGT AAAGTCCAAA CCAGTGTCTG ACTTACCTTC AGCACCAGCT 
TCTGAGGACA TGAAGCCTCA GCCTGCACCT GCTAATTCTC CCCAATCAGC TCGTGACACT 
GGGAAGCCAA AACCAGTTTT CGATAATTCT TCCAGATCAG TTTCTGAGGA TGAGAAGCCC 
AAAGGGATGT CTTCTGATTC TCCCAGGCCA GTCTTTGAGG ATACCGGTCA CAAGCCGGTC 
TCCTCTAATT CTCCCAAACC AGTTCCTGAG GAAGTGAAAG CGACCTATTG GCCTCGACCC 
CAGTTGGTCA CAGGATACTG TACATATCTT CCCTTTCGGG GATTTGACAT TACCCAGACT 
CCACCAGCCT TCATCAGTGT GTTACCGACT TTGCCCCAGT ATAGCATTTA CACCCCTCTG 
GCCAGTGTTC CTACTGAGTA TCACCTGCAA AGACCAATAC CAGTGGTGCC GTCTTTTGTA 
GCCAGTGACA TAACGGAAGT GCATTTTGAG GCTCATCATT TCAGTGCTGA GAATGCCCCT 
GGTACCCAGA TTGCCTCCGA AACACAGATC CTTGAGGAAT CTTTGGAAAC ACCAGTGAAG 
TCAGAGTGCA GCTATGATGG TGCTGATATA GTCCAGAGTG AGGCTAGCAG AAACGATGAC 
TACTGTGGCG ATTCTGCCAA ATGGGAAGTA AATCCAGAAA GCACCAATGA AGTAACAAAT 
ATTCCATGTA CACAAGTGGT TGCTGTACAG GTGTCTTGGA ATATAACACA TCAGGAAGTC 
AATACTGAGC CATATGGTCC TTTTGAGGCA CAACAGGGGG GTATTTCACA GGTTGAAAAG 
GAGTACCAGG TTTTACAAGA GCAGCTTAAG GAAGCATGTG AAAATTATGA ACACAGCAAT 
GTCAAGGGCT CAGAAGAAAC CAAGGACTTG GAAGAAAAGT TGAAAAGGAA TGTTGAAGAA 
AATAAGATCT CAAAGACAGA ACTAGATTGG TTCCTTGAAG ATTTGAACAA GGAAATTAAA 
AAATGGGAAC AGGAGAAAAA AGAAATCCAA GAAAGACTAA AGGCACTGAA GAAGAAAATG 
AAAAAGGTGT TAAATGCCAG CAAAATGTAT ACGCAGGAAA ATGATGGAAA GGAAAAGGCA 
CTTGAATTAC TTCTGGATCA GTCCCTTGAA ATCAGTAATA CATTTACAAA TGAGAAAATG 
AAAATAGAAG AGAGTATAAA GAAAGGGAAA GAGCATTATG AAGAGAGTCT TCAAAGGGCT 
GTGGATGCAG AGGTATCTGT GCTTGAAAAC TGGAAGGACA TGGAAGTGTG TAAGTTACAG 
AGCATGGAGT CACAAGCCGA AAGGTTTCTC AAGAATCTGA AGCTAATGAG CAGTGATTCG 
GCAGCGTATT CTGACATGGA CTCAGACATA CATTCATGGG AATCGTTTCT TTCTGATGTT 
AGAGAAGAAA TTGAGAAAGC AAAGTCTCAG TTTGAAGAAC AAATTCAGGC AATTAAATAT 
GGTTCTCAGC TCAGTGAACT TCCTAAAGTG CAGATTTCTG AGCTTTCATT TCCTACCTGT 
AACACGATTC ATCCTAAATT ACTTCCTGAG TCTTCAGGCC AGGATGGTCA AGGTCCCTCC 
ACTTCTACAA GTCATGGGAC CGGAGACCAG ACAGCAGCTC CCGAGGAGTC AAGTCTGGGC 
TCTGCAGCTG ACAGCCCAGT GGAGTTGCCC TCCTGCAGTC AGAAAGCCCC CCAGCTGCCA 
GAGCATGAGG GTGCTGGCCA GGCAACTGGG CCAGAACCAG GCCCAGTGGC TGATCAGAAG 
TTACCTGCTG CTCCAGAGCG TGCCACTCAT TCAAGCCAGT CTCCAAAGAA GCCGTTCAAT 
AGCATTATTG AGCACCTGTT GGTGGTGTTC CCGTGTTACA GCAGCACGGA GCTTGCTGGT 
TTTATTAAAA AAGTTAGAAA CAAAAACAAG ACCTCTCTGT CTGGATTGAG TATTGACGAA 
ATTGTGCAAA GAGTGACAGA ACACGTCCTT GATGAAGAAA GAAAGAAAAA GCCAAGTCCA 
GGAAAGGGCT GGAGGCTGTC TGCACCCAGC TCTGCTGTTG CTGTGCCCAG GGCTGCCCAG 
GACCCGCCCT CCATGCCTGT CGGAGCATCG CCCGAAACCA GGGGGCCGAG GACCGAGGAC 
GTCCCTGTGT CCTGCGCAAA TTCCTGTGAA ATGTGCCGAG AAGTATTCAA GTCCAAACAC 
GTGCGTGAGC TGCAGTGTGG GCACAGGCTT CACAAGGGGT GCTTCAAGCA GTGGCTGAAG 
AGCCAGCGCA CCTGCCCAGC CTGCCTGGGT CACGACCTCC TGTCAGAGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_010801674.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010799976.1 Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X4, mRNA.

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CloneID	OBa46601
Clone ID Related Accession (Same CDS sequence)	XM_010801674.1
Accession Version	XM_010801674.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5865bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TTC3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103826.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGATT TTCCCCAGGG AAATTTTGCA GATTATGCCT TGTTAGAAGA TTGCCCTTAT 
GTGGATGATT GTATCTTTGC CCCTGAGTTT ATGTCCAGTG ATTATGTTCG TGTGACTCAG 
CTTTACTGTG ATGGAGTGGG TAGACAGTAT AAAGATTATG CCCAAACTGA GGGAAACTTA 
GAATTTGACA TCTGCACTCT GTGGTGTAGT CAACCTATTT CTGTCCTGCA AGATTACTGT 
GATGCCATTA AAGTCCACAT CTTCTGGCCA CTTCTGTTTC AACGTCAGCA GAGCTCTATA 
ATAACACGGA TGCACTCCTG TGTAAGGACC AGCGGTTCTC ATGCTTGTGA GATAAGTTTG 
AAGAAATTAC AGCAGCTTGA ACTGATGGAA GATATTGTGG ATTTGGCAAA GAAAGTTGTG 
AATGATACAT TCTTTATCGG AGGCTTATTG AGAATTGGCT ATAAAATAGA GAATAAAATC 
TTGGCAATGG AAGAAGCTTT GAATTGGGTG AAATACACAG GCGATGTAAC AATTTTATCT 
AAATTAGGAG CAGTTGATAA TTGTTGGCGT ATGTTAAGTA TTTTCTTTAC TGAATACAAG 
TATCACATAA CTAAAGTTGT AACAGAAAAC TGCAGTTTGC TTGAAGAATT TAAAACTCAA 
AGTTGTGCGG AGTGTCTAGA GCAAGGAGAA CTAATGAAAA TGAAAGGAAA TGAAGAGTTT 
TCCAAAGAAA GATATGATAT AGCTATTATC TATTACACCA GAGCCATTGA ATGTAGGCCT 
GAAAATCACC TGCTTTATGG TAACCGAGCT CTTTGTTTTC TTCGTACTGG ACAATTCAGA 
AATGCACTTG GTGATGGAAA GAGAGCTACC ATTCTGAAGA ACAATTGGAC AAAGGGTCAT 
TATCGTTATT GTGCTGCTCT TTCTATGCTG GGGGAATATG ACTGGGCCCT GCAAGCAAAC 
ATAAAAGCTC AAAAACTCTG TAAAAATGAC CCTGAGGGAA TCAAGGATCT AATTCAGCAG 
CATGTAAAGT TACAAAAACA AATAGAAGAC CTGCAAGGTC AAACACCAGC TAGTAATCCA 
GTCAAAGCCT TCTGTGAAAG CAGGGCCTAC ATACCTAGTT TATCAGCACC TGCATTTAGT 
ACTTCACTTA ACTTTGTGGA AACTGAAAAA AATTTCAGAA AAACTAACCA CGAAATGGCA 
AACGGTGGTA ATCAGAATCC AAAGGTGGTA GATGAAGCTC TGAAAGTAGA TGATTGTGAC 
TGTCATCCTG AATTTGTACC GCCATCAAGT CAGCCTCCAA AATATAAAGG GAAACAAAAA 
TCTCGAAACA ATGAATTGGA GAAGTTCAGC CCTGGTTCAC AAGAAACTTT ACCAGTAGAT 
TTAAAAAACA TCTTGGAGAA ACAGTTTTCT AAATCTTCCA GAGCCGCACA CCAGGATTTT 
GCTAATATAA TGAAAATGCT GAGAAGTTTA ATTCAGGATG GCTACACGGC CTTGCTGGAG 
CAGCGCTGCC GCAGCGCCGC CCAAGCCTTC ACAGAGTTGC TCAACGGTTT AGATCCTCAA 
AAAATAAAGC AATTGAACCT GGCCATGATT AACTATGTGT TAGTAGTCTA CGGACTTGCC 
ATTTCACTCC TTGGAATAGG ACAGCCCGAG GAGTTATCTG AAGCTGAAAA CCAGTTCAAG 
AGGATGATTG AACACTACCC CAATGAGGGG CTTGATTGCT TGGCCTACTG TGGAATTGGA 
AAAGTGTATT TGAAAAAAAA CAGATTTTTA GAAGCTCTTA ATCATTTTGA GAAAGCAAGA 
ACCTTGATCT GCCGTCTTCC TGGAGTGTTA ACTTGGCCCA CAAGTAATGT GGTTATTGAG 
GAGACTCAGC CAGGGAAGAT AAAGATGCTG TTAGAGAAAT TTGTTGAAGA ATGCAGGTTC 
CCTCCAGTGC CGGATGCCAC TTGTTGCTAT CAGAAGTGCC ATGGATATTC CAAAATCCAG 
ATATATATAA CTGATCCAGA CTTTAAGGGT TTTATACGGA TCAGCTGTTG CCAGTATTGT 
AAAATAGAAT TTCACATGAA CTGCTGGAAG AAGTTAAAAA CAACAACCTT CAATGATAAA 
ATTGACAAGG ATTTTCTGCA AGGAATCTGT CTTACTCCTG ATTGTGAAGG AATCATTTCA 
AAGATTATCA TCTTCAGCAG TGGTGGTCAA GTTAAATGTG AATTTGAACA CAAGGTCATT 
AAAGAAAAGG TTCCTCCAAG ACCTATTCTG AAACAGAAAT GTTCTAGCCT AGAGAAGTTG 
AGGCTGAAAG AAGACAAAAA ATTGAAGCGA AAGCTCCAAA AAAAGGAAGC AAAAAAATTA 
GCACAAGAAA GACTGGAAGA GGACTTAAGG GAAAGTAACC CACCAAAAAC TGAAGAGCAG 
AGAGAAACTA TCAACCATGT CCAAAGTTGC CAGTTCCTTG ATGACAGAAT TCTGCAGTGC 
ATAAAGCAGT ACGCTGACAA GATTAAGTCC GGGATACTGA ATACTTCCAA GCTTCTCAAA 
GAATTGCTTT CTTGGAAGGT TTTAACCACA GAAGACTACA CAACGTGTTT TTCTAGCAGA 
AATTTCCTGA ATGAAGCAGT GGACTATGTC ATTCGTCATT TGATCCAAGA AAAGAATAGA 
GTAAAGACAA GGGTATTTCT GCATGTTTTG AGTGAGCTTA AAGAAGTGGA CCCAAAACTA 
GCCACCTGGA TCCAGAAACT GAATAGCTTT GGCTTAGATG CCACAGGACC TTTCTTTTCT 
CGTTATGGGG CTCCTCTTAA GGAGCTTGAT TTTAGCATCA TGACTTTTCT CTGGAATGAG 
AAATACGGTC ATAAGCTAGG CTCTATAGAA GGAAAGCAAC TTGATTATTT CTATGAGCCT 
ACATCACTGA AGGAAGCGCG GTGTTTAATA TGGCTGCTAG AAGAACACAG AGACAAGTTC 
CCAGCACTGC ATAATGCCTT GGACGAGTTC TTTGATATAA TGGACAGCCG CTGCACTGTA 
TTAAGGAAAC AGGACAGTGG TGACGTGCCA TTTAATTCTA CCAAGATAAA AAACAAAGGC 
AAGAAAAAGA AGCCAAAAGA TTCAAAGCCT ATGTTAGTTG GATCTGGAAC ACCTTCAGTC 
ACACCAAACA ATGAGACTGT CACTTCAGGT GAAGACCATA TCAAACGCAG TTTAAGTTCT 
GCGGGCCCCT TTGTAGTGCC TGATCACCTT CGGCAGGATA TAGAAGAATT TGAAGCTCTG 
TATGAACAGC ATAGTAATGA GTATGTTGTC CGTAATAAGA AGCTGTGGGA CATAAACCCA 
AAACAGAAAT GTTCAACTCT ATACGATTAT TTCTCTCAGT TGTTGGAGGA ACATGGTCCC 
TTGGACATGA GTAACAAAAT GTTTTCTGAA GAATATGAGT TCTTCCCAGA AGAAACTCAA 
CAGATACTAG AAAAAGCAGG AGGCTTGAAA GCGTTTCTCC TGGGATGTCC TCGTTTTGTT 
GTGATTGATA ACTGTATCGC ATTGAAGAAA GTTGCATTAC GGCTCAAGAA AAAGAGAAAG 
AAGAAAAACA TTAAAACAAA AGTAGAAGAA ATTTCAAAAA CAGGAGAGTA TTTACGAGTT 
AAACTACCAC TGAATCCAAC TGCTAGGGAA TTTAAACCAG ATGTAAAGTC CAAACCAGTG 
TCTGACTTAC CTTCAGCACC AGCTTCTGAG GACATGAAGC CTCAGCCTGC ACCTGCTAAT 
TCTCCCCAAT CAGCTCGTGA CACTGGGAAG CCAAAACCAG TTTTCGATAA TTCTTCCAGA 
TCAGTTTCTG AGGATGAGAA GCCCAAAGGG ATGTCTTCTG ATTCTCCCAG GCCAGTCTTT 
GAGGATACCG GTCACAAGCC GGTCTCCTCT AATTCTCCCA AACCAGTTCC TGAGGAAGTG 
AAAGCGACCT ATTGGCCTCG ACCCCAGTTG GTCACAGGAT ACTGTACATA TCTTCCCTTT 
CGGGGATTTG ACATTACCCA GACTCCACCA GCCTTCATCA GTGTGTTACC GACTTTGCCC 
CAGTATAGCA TTTACACCCC TCTGGCCAGT GTTCCTACTG AGTATCACCT GCAAAGACCA 
ATACCAGTGG TGCCGTCTTT TGTAGCCAGT GACATAACGG AAGTGCATTT TGAGGCTCAT 
CATTTCAGTG CTGAGAATGC CCCTGGTACC CAGATTGCCT CCGAAACACA GATCCTTGAG 
GAATCTTTGG AAACACCAGT GAAGTCAGAG TGCAGCTATG ATGGTGCTGA TATAGTCCAG 
AGTGAGGCTA GCAGAAACGA TGACTACTGT GGCGATTCTG CCAAATGGGA AGTAAATCCA 
GAAAGCACCA ATGAAGTAAC AAATATTCCA TGTACACAAG TGGTTGCTGT ACAGGTGTCT 
TGGAATATAA CACATCAGGA AGTCAATACT GAGCCATATG GTCCTTTTGA GGCACAACAG 
GGGGGTATTT CACAGGTTGA AAAGGAGTAC CAGGTTTTAC AAGAGCAGCT TAAGGAAGCA 
TGTGAAAATT ATGAACACAG CAATGTCAAG GGCTCAGAAG AAACCAAGGA CTTGGAAGAA 
AAGTTGAAAA GGAATGTTGA AGAAAATAAG ATCTCAAAGA CAGAACTAGA TTGGTTCCTT 
GAAGATTTGA ACAAGGAAAT TAAAAAATGG GAACAGGAGA AAAAAGAAAT CCAAGAAAGA 
CTAAAGGCAC TGAAGAAGAA AATGAAAAAG GTGTTAAATG CCAGCAAAAT GTATACGCAG 
GAAAATGATG GAAAGGAAAA GGCACTTGAA TTACTTCTGG ATCAGTCCCT TGAAATCAGT 
AATACATTTA CAAATGAGAA AATGAAAATA GAAGAGAGTA TAAAGAAAGG GAAAGAGCAT 
TATGAAGAGA GTCTTCAAAG GGCTGTGGAT GCAGAGGTAT CTGTGCTTGA AAACTGGAAG 
GACATGGAAG TGTGTAAGTT ACAGAGCATG GAGTCACAAG CCGAAAGGTT TCTCAAGAAT 
CTGAAGCTAA TGAGCAGTGA TTCGGCAGCG TATTCTGACA TGGACTCAGA CATACATTCA 
TGGGAATCGT TTCTTTCTGA TGTTAGAGAA GAAATTGAGA AAGCAAAGTC TCAGTTTGAA 
GAACAAATTC AGGCAATTAA ATATGGTTCT CAGCTCAGTG AACTTCCTAA AGTGCAGATT 
TCTGAGCTTT CATTTCCTAC CTGTAACACG ATTCATCCTA AATTACTTCC TGAGTCTTCA 
GGCCAGGATG GTCAAGGTCC CTCCACTTCT ACAAGTCATG GGACCGGAGA CCAGACAGCA 
GCTCCCGAGG AGTCAAGTCT GGGCTCTGCA GCTGACAGCC CAGTGGAGTT GCCCTCCTGC 
AGTCAGAAAG CCCCCCAGCT GCCAGAGCAT GAGGGTGCTG GCCAGGCAAC TGGGCCAGAA 
CCAGGCCCAG TGGCTGATCA GAAGTTACCT GCTGCTCCAG AGCGTGCCAC TCATTCAAGC 
CAGTCTCCAA AGAAGCCGTT CAATAGCATT ATTGAGCACC TGTTGGTGGT GTTCCCGTGT 
TACAGCAGCA CGGAGCTTGC TGGTTTTATT AAAAAAGTTA GAAACAAAAA CAAGACCTCT 
CTGTCTGGAT TGAGTATTGA CGAAATTGTG CAAAGAGTGA CAGAACACGT CCTTGATGAA 
GAAAGAAAGA AAAAGCCAAG TCCAGGAAAG GGCTGGAGGC TGTCTGCACC CAGCTCTGCT 
GTTGCTGTGC CCAGGGCTGC CCAGGACCCG CCCTCCATGC CTGTCGGAGC ATCGCCCGAA 
ACCAGGGGGC CGAGGACCGA GGACGTCCCT TGCTTCAAGC AGTGGCTGAA GAGCCAGCGC 
ACCTGCCCAG CCTGCCTGGG TCACGACCTC CTGTCAGAGG AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010799976.1
CDS	12..5876
Translation	MADFPQGNFADYALLEDCPYVDDCIFAPEFMSSDYVRVTQLYCDGVGRQYKDYAQTEGNLEFDICTLWCSQPISVLQDYCDAIKVHIFWPLLFQRQQSSIITRMHSCVRTSGSHACEISLKKLQQLELMEDIVDLAKKVVNDTFFIGGLLRIGYKIENKILAMEEALNWVKYTGDVTILSKLGAVDNCWRMLSIFFTEYKYHITKVVTENCSLLEEFKTQSCAECLEQGELMKMKGNEEFSKERYDIAIIYYTRAIECRPENHLLYGNRALCFLRTGQFRNALGDGKRATILKNNWTKGHYRYCAALSMLGEYDWALQANIKAQKLCKNDPEGIKDLIQQHVKLQKQIEDLQGQTPASNPVKAFCESRAYIPSLSAPAFSTSLNFVETEKNFRKTNHEMANGGNQNPKVVDEALKVDDCDCHPEFVPPSSQPPKYKGKQKSRNNELEKFSPGSQETLPVDLKNILEKQFSKSSRAAHQDFANIMKMLRSLIQDGYTALLEQRCRSAAQAFTELLNGLDPQKIKQLNLAMINYVLVVYGLAISLLGIGQPEELSEAENQFKRMIEHYPNEGLDCLAYCGIGKVYLKKNRFLEALNHFEKARTLICRLPGVLTWPTSNVVIEETQPGKIKMLLEKFVEECRFPPVPDATCCYQKCHGYSKIQIYITDPDFKGFIRISCCQYCKIEFHMNCWKKLKTTTFNDKIDKDFLQGICLTPDCEGIISKIIIFSSGGQVKCEFEHKVIKEKVPPRPILKQKCSSLEKLRLKEDKKLKRKLQKKEAKKLAQERLEEDLRESNPPKTEEQRETINHVQSCQFLDDRILQCIKQYADKIKSGILNTSKLLKELLSWKVLTTEDYTTCFSSRNFLNEAVDYVIRHLIQEKNRVKTRVFLHVLSELKEVDPKLATWIQKLNSFGLDATGPFFSRYGAPLKELDFSIMTFLWNEKYGHKLGSIEGKQLDYFYEPTSLKEARCLIWLLEEHRDKFPALHNALDEFFDIMDSRCTVLRKQDSGDVPFNSTKIKNKGKKKKPKDSKPMLVGSGTPSVTPNNETVTSGEDHIKRSLSSAGPFVVPDHLRQDIEEFEALYEQHSNEYVVRNKKLWDINPKQKCSTLYDYFSQLLEEHGPLDMSNKMFSEEYEFFPEETQQILEKAGGLKAFLLGCPRFVVIDNCIALKKVALRLKKKRKKKNIKTKVEEISKTGEYLRVKLPLNPTAREFKPDVKSKPVSDLPSAPASEDMKPQPAPANSPQSARDTGKPKPVFDNSSRSVSEDEKPKGMSSDSPRPVFEDTGHKPVSSNSPKPVPEEVKATYWPRPQLVTGYCTYLPFRGFDITQTPPAFISVLPTLPQYSIYTPLASVPTEYHLQRPIPVVPSFVASDITEVHFEAHHFSAENAPGTQIASETQILEESLETPVKSECSYDGADIVQSEASRNDDYCGDSAKWEVNPESTNEVTNIPCTQVVAVQVSWNITHQEVNTEPYGPFEAQQGGISQVEKEYQVLQEQLKEACENYEHSNVKGSEETKDLEEKLKRNVEENKISKTELDWFLEDLNKEIKKWEQEKKEIQERLKALKKKMKKVLNASKMYTQENDGKEKALELLLDQSLEISNTFTNEKMKIEESIKKGKEHYEESLQRAVDAEVSVLENWKDMEVCKLQSMESQAERFLKNLKLMSSDSAAYSDMDSDIHSWESFLSDVREEIEKAKSQFEEQIQAIKYGSQLSELPKVQISELSFPTCNTIHPKLLPESSGQDGQGPSTSTSHGTGDQTAAPEESSLGSAADSPVELPSCSQKAPQLPEHEGAGQATGPEPGPVADQKLPAAPERATHSSQSPKKPFNSIIEHLLVVFPCYSSTELAGFIKKVRNKNKTSLSGLSIDEIVQRVTEHVLDEERKKKPSPGKGWRLSAPSSAVAVPRAAQDPPSMPVGASPETRGPRTEDVPCFKQWLKSQRTCPACLGHDLLSEE

Target ORF information:

RefSeq Version	XM_010801674.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010801674.1

ORF Insert Sequence:

ATGGCCGATT TTCCCCAGGG AAATTTTGCA GATTATGCCT TGTTAGAAGA TTGCCCTTAT 
GTGGATGATT GTATCTTTGC CCCTGAGTTT ATGTCCAGTG ATTATGTTCG TGTGACTCAG 
CTTTACTGTG ATGGAGTGGG TAGACAGTAT AAAGATTATG CCCAAACTGA GGGAAACTTA 
GAATTTGACA TCTGCACTCT GTGGTGTAGT CAACCTATTT CTGTCCTGCA AGATTACTGT 
GATGCCATTA AAGTCCACAT CTTCTGGCCA CTTCTGTTTC AACGTCAGCA GAGCTCTATA 
ATAACACGGA TGCACTCCTG TGTAAGGACC AGCGGTTCTC ATGCTTGTGA GATAAGTTTG 
AAGAAATTAC AGCAGCTTGA ACTGATGGAA GATATTGTGG ATTTGGCAAA GAAAGTTGTG 
AATGATACAT TCTTTATCGG AGGCTTATTG AGAATTGGCT ATAAAATAGA GAATAAAATC 
TTGGCAATGG AAGAAGCTTT GAATTGGGTG AAATACACAG GCGATGTAAC AATTTTATCT 
AAATTAGGAG CAGTTGATAA TTGTTGGCGT ATGTTAAGTA TTTTCTTTAC TGAATACAAG 
TATCACATAA CTAAAGTTGT AACAGAAAAC TGCAGTTTGC TTGAAGAATT TAAAACTCAA 
AGTTGTGCGG AGTGTCTAGA GCAAGGAGAA CTAATGAAAA TGAAAGGAAA TGAAGAGTTT 
TCCAAAGAAA GATATGATAT AGCTATTATC TATTACACCA GAGCCATTGA ATGTAGGCCT 
GAAAATCACC TGCTTTATGG TAACCGAGCT CTTTGTTTTC TTCGTACTGG ACAATTCAGA 
AATGCACTTG GTGATGGAAA GAGAGCTACC ATTCTGAAGA ACAATTGGAC AAAGGGTCAT 
TATCGTTATT GTGCTGCTCT TTCTATGCTG GGGGAATATG ACTGGGCCCT GCAAGCAAAC 
ATAAAAGCTC AAAAACTCTG TAAAAATGAC CCTGAGGGAA TCAAGGATCT AATTCAGCAG 
CATGTAAAGT TACAAAAACA AATAGAAGAC CTGCAAGGTC AAACACCAGC TAGTAATCCA 
GTCAAAGCCT TCTGTGAAAG CAGGGCCTAC ATACCTAGTT TATCAGCACC TGCATTTAGT 
ACTTCACTTA ACTTTGTGGA AACTGAAAAA AATTTCAGAA AAACTAACCA CGAAATGGCA 
AACGGTGGTA ATCAGAATCC AAAGGTGGTA GATGAAGCTC TGAAAGTAGA TGATTGTGAC 
TGTCATCCTG AATTTGTACC GCCATCAAGT CAGCCTCCAA AATATAAAGG GAAACAAAAA 
TCTCGAAACA ATGAATTGGA GAAGTTCAGC CCTGGTTCAC AAGAAACTTT ACCAGTAGAT 
TTAAAAAACA TCTTGGAGAA ACAGTTTTCT AAATCTTCCA GAGCCGCACA CCAGGATTTT 
GCTAATATAA TGAAAATGCT GAGAAGTTTA ATTCAGGATG GCTACACGGC CTTGCTGGAG 
CAGCGCTGCC GCAGCGCCGC CCAAGCCTTC ACAGAGTTGC TCAACGGTTT AGATCCTCAA 
AAAATAAAGC AATTGAACCT GGCCATGATT AACTATGTGT TAGTAGTCTA CGGACTTGCC 
ATTTCACTCC TTGGAATAGG ACAGCCCGAG GAGTTATCTG AAGCTGAAAA CCAGTTCAAG 
AGGATGATTG AACACTACCC CAATGAGGGG CTTGATTGCT TGGCCTACTG TGGAATTGGA 
AAAGTGTATT TGAAAAAAAA CAGATTTTTA GAAGCTCTTA ATCATTTTGA GAAAGCAAGA 
ACCTTGATCT GCCGTCTTCC TGGAGTGTTA ACTTGGCCCA CAAGTAATGT GGTTATTGAG 
GAGACTCAGC CAGGGAAGAT AAAGATGCTG TTAGAGAAAT TTGTTGAAGA ATGCAGGTTC 
CCTCCAGTGC CGGATGCCAC TTGTTGCTAT CAGAAGTGCC ATGGATATTC CAAAATCCAG 
ATATATATAA CTGATCCAGA CTTTAAGGGT TTTATACGGA TCAGCTGTTG CCAGTATTGT 
AAAATAGAAT TTCACATGAA CTGCTGGAAG AAGTTAAAAA CAACAACCTT CAATGATAAA 
ATTGACAAGG ATTTTCTGCA AGGAATCTGT CTTACTCCTG ATTGTGAAGG AATCATTTCA 
AAGATTATCA TCTTCAGCAG TGGTGGTCAA GTTAAATGTG AATTTGAACA CAAGGTCATT 
AAAGAAAAGG TTCCTCCAAG ACCTATTCTG AAACAGAAAT GTTCTAGCCT AGAGAAGTTG 
AGGCTGAAAG AAGACAAAAA ATTGAAGCGA AAGCTCCAAA AAAAGGAAGC AAAAAAATTA 
GCACAAGAAA GACTGGAAGA GGACTTAAGG GAAAGTAACC CACCAAAAAC TGAAGAGCAG 
AGAGAAACTA TCAACCATGT CCAAAGTTGC CAGTTCCTTG ATGACAGAAT TCTGCAGTGC 
ATAAAGCAGT ACGCTGACAA GATTAAGTCC GGGATACTGA ATACTTCCAA GCTTCTCAAA 
GAATTGCTTT CTTGGAAGGT TTTAACCACA GAAGACTACA CAACGTGTTT TTCTAGCAGA 
AATTTCCTGA ATGAAGCAGT GGACTATGTC ATTCGTCATT TGATCCAAGA AAAGAATAGA 
GTAAAGACAA GGGTATTTCT GCATGTTTTG AGTGAGCTTA AAGAAGTGGA CCCAAAACTA 
GCCACCTGGA TCCAGAAACT GAATAGCTTT GGCTTAGATG CCACAGGACC TTTCTTTTCT 
CGTTATGGGG CTCCTCTTAA GGAGCTTGAT TTTAGCATCA TGACTTTTCT CTGGAATGAG 
AAATACGGTC ATAAGCTAGG CTCTATAGAA GGAAAGCAAC TTGATTATTT CTATGAGCCT 
ACATCACTGA AGGAAGCGCG GTGTTTAATA TGGCTGCTAG AAGAACACAG AGACAAGTTC 
CCAGCACTGC ATAATGCCTT GGACGAGTTC TTTGATATAA TGGACAGCCG CTGCACTGTA 
TTAAGGAAAC AGGACAGTGG TGACGTGCCA TTTAATTCTA CCAAGATAAA AAACAAAGGC 
AAGAAAAAGA AGCCAAAAGA TTCAAAGCCT ATGTTAGTTG GATCTGGAAC ACCTTCAGTC 
ACACCAAACA ATGAGACTGT CACTTCAGGT GAAGACCATA TCAAACGCAG TTTAAGTTCT 
GCGGGCCCCT TTGTAGTGCC TGATCACCTT CGGCAGGATA TAGAAGAATT TGAAGCTCTG 
TATGAACAGC ATAGTAATGA GTATGTTGTC CGTAATAAGA AGCTGTGGGA CATAAACCCA 
AAACAGAAAT GTTCAACTCT ATACGATTAT TTCTCTCAGT TGTTGGAGGA ACATGGTCCC 
TTGGACATGA GTAACAAAAT GTTTTCTGAA GAATATGAGT TCTTCCCAGA AGAAACTCAA 
CAGATACTAG AAAAAGCAGG AGGCTTGAAA GCGTTTCTCC TGGGATGTCC TCGTTTTGTT 
GTGATTGATA ACTGTATCGC ATTGAAGAAA GTTGCATTAC GGCTCAAGAA AAAGAGAAAG 
AAGAAAAACA TTAAAACAAA AGTAGAAGAA ATTTCAAAAA CAGGAGAGTA TTTACGAGTT 
AAACTACCAC TGAATCCAAC TGCTAGGGAA TTTAAACCAG ATGTAAAGTC CAAACCAGTG 
TCTGACTTAC CTTCAGCACC AGCTTCTGAG GACATGAAGC CTCAGCCTGC ACCTGCTAAT 
TCTCCCCAAT CAGCTCGTGA CACTGGGAAG CCAAAACCAG TTTTCGATAA TTCTTCCAGA 
TCAGTTTCTG AGGATGAGAA GCCCAAAGGG ATGTCTTCTG ATTCTCCCAG GCCAGTCTTT 
GAGGATACCG GTCACAAGCC GGTCTCCTCT AATTCTCCCA AACCAGTTCC TGAGGAAGTG 
AAAGCGACCT ATTGGCCTCG ACCCCAGTTG GTCACAGGAT ACTGTACATA TCTTCCCTTT 
CGGGGATTTG ACATTACCCA GACTCCACCA GCCTTCATCA GTGTGTTACC GACTTTGCCC 
CAGTATAGCA TTTACACCCC TCTGGCCAGT GTTCCTACTG AGTATCACCT GCAAAGACCA 
ATACCAGTGG TGCCGTCTTT TGTAGCCAGT GACATAACGG AAGTGCATTT TGAGGCTCAT 
CATTTCAGTG CTGAGAATGC CCCTGGTACC CAGATTGCCT CCGAAACACA GATCCTTGAG 
GAATCTTTGG AAACACCAGT GAAGTCAGAG TGCAGCTATG ATGGTGCTGA TATAGTCCAG 
AGTGAGGCTA GCAGAAACGA TGACTACTGT GGCGATTCTG CCAAATGGGA AGTAAATCCA 
GAAAGCACCA ATGAAGTAAC AAATATTCCA TGTACACAAG TGGTTGCTGT ACAGGTGTCT 
TGGAATATAA CACATCAGGA AGTCAATACT GAGCCATATG GTCCTTTTGA GGCACAACAG 
GGGGGTATTT CACAGGTTGA AAAGGAGTAC CAGGTTTTAC AAGAGCAGCT TAAGGAAGCA 
TGTGAAAATT ATGAACACAG CAATGTCAAG GGCTCAGAAG AAACCAAGGA CTTGGAAGAA 
AAGTTGAAAA GGAATGTTGA AGAAAATAAG ATCTCAAAGA CAGAACTAGA TTGGTTCCTT 
GAAGATTTGA ACAAGGAAAT TAAAAAATGG GAACAGGAGA AAAAAGAAAT CCAAGAAAGA 
CTAAAGGCAC TGAAGAAGAA AATGAAAAAG GTGTTAAATG CCAGCAAAAT GTATACGCAG 
GAAAATGATG GAAAGGAAAA GGCACTTGAA TTACTTCTGG ATCAGTCCCT TGAAATCAGT 
AATACATTTA CAAATGAGAA AATGAAAATA GAAGAGAGTA TAAAGAAAGG GAAAGAGCAT 
TATGAAGAGA GTCTTCAAAG GGCTGTGGAT GCAGAGGTAT CTGTGCTTGA AAACTGGAAG 
GACATGGAAG TGTGTAAGTT ACAGAGCATG GAGTCACAAG CCGAAAGGTT TCTCAAGAAT 
CTGAAGCTAA TGAGCAGTGA TTCGGCAGCG TATTCTGACA TGGACTCAGA CATACATTCA 
TGGGAATCGT TTCTTTCTGA TGTTAGAGAA GAAATTGAGA AAGCAAAGTC TCAGTTTGAA 
GAACAAATTC AGGCAATTAA ATATGGTTCT CAGCTCAGTG AACTTCCTAA AGTGCAGATT 
TCTGAGCTTT CATTTCCTAC CTGTAACACG ATTCATCCTA AATTACTTCC TGAGTCTTCA 
GGCCAGGATG GTCAAGGTCC CTCCACTTCT ACAAGTCATG GGACCGGAGA CCAGACAGCA 
GCTCCCGAGG AGTCAAGTCT GGGCTCTGCA GCTGACAGCC CAGTGGAGTT GCCCTCCTGC 
AGTCAGAAAG CCCCCCAGCT GCCAGAGCAT GAGGGTGCTG GCCAGGCAAC TGGGCCAGAA 
CCAGGCCCAG TGGCTGATCA GAAGTTACCT GCTGCTCCAG AGCGTGCCAC TCATTCAAGC 
CAGTCTCCAA AGAAGCCGTT CAATAGCATT ATTGAGCACC TGTTGGTGGT GTTCCCGTGT 
TACAGCAGCA CGGAGCTTGC TGGTTTTATT AAAAAAGTTA GAAACAAAAA CAAGACCTCT 
CTGTCTGGAT TGAGTATTGA CGAAATTGTG CAAAGAGTGA CAGAACACGT CCTTGATGAA 
GAAAGAAAGA AAAAGCCAAG TCCAGGAAAG GGCTGGAGGC TGTCTGCACC CAGCTCTGCT 
GTTGCTGTGC CCAGGGCTGC CCAGGACCCG CCCTCCATGC CTGTCGGAGC ATCGCCCGAA 
ACCAGGGGGC CGAGGACCGA GGACGTCCCT TGCTTCAAGC AGTGGCTGAA GAGCCAGCGC 
ACCTGCCCAG CCTGCCTGGG TCACGACCTC CTGTCAGAGG AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_010801675.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010799977.1 Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X5, mRNA.

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CloneID	OBa46602
Clone ID Related Accession (Same CDS sequence)	XM_010801675.1
Accession Version	XM_010801675.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5823bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TTC3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103826.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGATT TTCCCCAGGG AAATTTTGCA GATTATGCCT TGTTAGAAGA TTGCCCTTAT 
GTGGATGATT GTATCTTTGC CCCTGAGTTT ATGTCCAGTG ATTATGTTCG TGTGACTCAG 
CTTTACTGTG ATGGAGTGGG TAGACAGTAT AAAGATTATG CCCAAACTGA GGGAAACTTA 
GAATTTGACA TCTGCACTCT GTGGTGTAGT CAACCTATTT CTGTCCTGCA AGATTACTGT 
GATGCCATTA AAGTCCACAT CTTCTGGCCA CTTCTGTTTC AACGTCAGCA GAGCTCTATA 
ATAACACGGA TGCACTCCTG TGTAAGGACC AGCGGTTCTC ATGCTTGTGA GATAAGTTTG 
AAGAAATTAC AGCAGCTTGA ACTGATGGAA GATATTGTGG ATTTGGCAAA GAAAGTTGTG 
AATGATACAT TCTTTATCGG AGGCTTATTG AGAATTGGCT ATAAAATAGA GAATAAAATC 
TTGGCAATGG AAGAAGCTTT GAATTGGGTG AAATACACAG GCGATGTAAC AATTTTATCT 
AAATTAGGAG CAGTTGATAA TTGTTGGCGT ATGTTAAGTA TTTTCTTTAC TGAATACAAG 
TATCACATAA CTAAAGTTGT AACAGAAAAC TGCAGTTTGC TTGAAGAATT TAAAACTCAA 
AGTTGTGCGG AGTGTCTAGA GCAAGGAGAA CTAATGAAAA TGAAAGGAAA TGAAGAGTTT 
TCCAAAGAAA GATATGATAT AGCTATTATC TATTACACCA GAGCCATTGA ATGTAGGCCT 
GAAAATCACC TGCTTTATGG TAACCGAGCT CTTTGTTTTC TTCGTACTGG ACAATTCAGA 
AATGCACTTG GTGATGGAAA GAGAGCTACC ATTCTGAAGA ACAATTGGAC AAAGGGTCAT 
TATCGTTATT GTGCTGCTCT TTCTATGCTG GGGGAATATG ACTGGGCCCT GCAAGCAAAC 
ATAAAAGCTC AAAAACTCTG TAAAAATGAC CCTGAGGGAA TCAAGGATCT AATTCAGCAG 
CATGTAAAGT TACAAAAACA AATAGAAGAC CTGCAAGGTC AAACACCAGC TAGTAATCCA 
GTCAAAGCCT TCTGTGAAAG CAGGGCCTAC ATACCTAGTT TATCAGCACC TGCATTTAGT 
ACTTCACTTA ACTTTGTGGA AACTGAAAAA AATTTCAGAA AAACTAACCA CGAAATGGCA 
AACGGTGGTA ATCAGAATCC AAAGGTGGTA GATGAAGCTC TGAAAGTAGA TGATTGTGAC 
TGTCATCCTG AATTTGTACC GCCATCAAGT CAGCCTCCAA AATATAAAGG GAAACAAAAA 
TCTCGAAACA ATGAATTGGA GAAGTTCAGC CCTGGTTCAC AAGAAACTTT ACCAGTAGAT 
TTAAAAAACA TCTTGGAGAA ACAGTTTTCT AAATCTTCCA GAGCCGCACA CCAGGATTTT 
GCTAATATAA TGAAAATGCT GAGAAGTTTA ATTCAGGATG GCTACACGGC CTTGCTGGAG 
CAGCGCTGCC GCAGCGCCGC CCAAGCCTTC ACAGAGTTGC TCAACGGTTT AGATCCTCAA 
AAAATAAAGC AATTGAACCT GGCCATGATT AACTATGTGT TAGTAGTCTA CGGACTTGCC 
ATTTCACTCC TTGGAATAGG ACAGCCCGAG GAGTTATCTG AAGCTGAAAA CCAGTTCAAG 
AGGATGATTG AACACTACCC CAATGAGGGG CTTGATTGCT TGGCCTACTG TGGAATTGGA 
AAAGTGTATT TGAAAAAAAA CAGATTTTTA GAAGCTCTTA ATCATTTTGA GAAAGCAAGA 
ACCTTGATCT GCCGTCTTCC TGGAGTGTTA ACTTGGCCCA CAAGTAATGT GGTTATTGAG 
GAGACTCAGC CAGGGAAGAT AAAGATGCTG TTAGAGAAAT TTGTTGAAGA ATGCAGGTTC 
CCTCCAGTGC CGGATGCCAC TTGTTGCTAT CAGAAGTGCC ATGGATATTC CAAAATCCAG 
ATATATATAA CTGATCCAGA CTTTAAGGGT TTTATACGGA TCAGCTGTTG CCAGTATTGT 
AAAATAGAAT TTCACATGAA CTGCTGGAAG AAGTTAAAAA CAACAACCTT CAATGATAAA 
ATTGACAAGG ATTTTCTGCA AGGAATCTGT CTTACTCCTG ATTGTGAAGG AATCATTTCA 
AAGATTATCA TCTTCAGCAG TGGTGGTCAA GTTAAATGTG AATTTGAACA CAAGGTCATT 
AAAGAAAAGG TTCCTCCAAG ACCTATTCTG AAACAGAAAT GTTCTAGCCT AGAGAAGTTG 
AGGCTGAAAG AAGACAAAAA ATTGAAGCGA AAGCTCCAAA AAAAGGAAGC AAAAAAATTA 
GCACAAGAAA GACTGGAAGA GGACTTAAGG GAAAGTAACC CACCAAAAAC TGAAGAGCAG 
AGAGAAACTA TCAACCATGT CCAAAGTTGC CAGTTCCTTG ATGACAGAAT TCTGCAGTGC 
ATAAAGCAGT ACGCTGACAA GATTAAGTCC GGGATACTGA ATACTTCCAA GCTTCTCAAA 
GAATTGCTTT CTTGGAAGGT TTTAACCACA GAAGACTACA CAACGTGTTT TTCTAGCAGA 
AATTTCCTGA ATGAAGCAGT GGACTATGTC ATTCGTCATT TGATCCAAGA AAAGAATAGA 
GTAAAGACAA GGGTATTTCT GCATGTTTTG AGTGAGCTTA AAGAAGTGGA CCCAAAACTA 
GCCACCTGGA TCCAGAAACT GAATAGCTTT GGCTTAGATG CCACAGGACC TTTCTTTTCT 
CGTTATGGGG CTCCTCTTAA GGAGCTTGAT TTTAGCATCA TGACTTTTCT CTGGAATGAG 
AAATACGGTC ATAAGCTAGG CTCTATAGAA GGAAAGCAAC TTGATTATTT CTATGAGCCT 
ACATCACTGA AGGAAGCGCG GTGTTTAATA TGGCTGCTAG AAGAACACAG AGACAAGTTC 
CCAGCACTGC ATAATGCCTT GGACGAGTTC TTTGATATAA TGGACAGCCG CTGCACTGTA 
TTAAGGAAAC AGGACAGTGG TGACGTGCCA TTTAATTCTA CCAAGATAAA AAACAAAGGC 
AAGAAAAAGA AGCCAAAAGA TTCAAAGCCT ATGTTAGTTG GATCTGGAAC ACCTTCAGTC 
ACACCAAACA ATGAGACTGT CACTTCAGGT GAAGACCATA TCAAACGCAG TTTAAGTTCT 
GCGGGCCCCT TTGTAGTGCC TGATCACCTT CGGCAGGATA TAGAAGAATT TGAAGCTCTG 
TATGAACAGC ATAGTAATGA GTATGTTGTC CGTAATAAGA AGCTGTGGGA CATAAACCCA 
AAACAGAAAT GTTCAACTCT ATACGATTAT TTCTCTCAGT TGTTGGAGGA ACATGGTCCC 
TTGGACATGA GTAACAAAAT GTTTTCTGAA GAATATGAGT TCTTCCCAGA AGAAACTCAA 
CAGATACTAG AAAAAGCAGG AGGCTTGAAA GCGTTTCTCC TGGGATGTCC TCGTTTTGTT 
GTGATTGATA ACTGTATCGC ATTGAAGAAA GTTGCATTAC GGCTCAAGAA AAAGAGAAAG 
AAGAAAAACA TTAAAACAAA AGTAGAAGAA ATTTCAAAAA CAGGAGAGTA TTTACGAGTT 
AAACTACCAC TGAATCCAAC TGCTAGGGAA TTTAAACCAG ATGTAAAGTC CAAACCAGTG 
TCTGACTTAC CTTCAGCACC AGCTTCTGAG GACATGAAGC CTCAGCCTGC ACCTGCTAAT 
TCTCCCCAAT CAGCTCGTGA CACTGGGAAG CCAAAACCAG TTTTCGATAA TTCTTCCAGA 
TCAGTTTCTG AGGATGAGAA GCCCAAAGGG ATGTCTTCTG ATTCTCCCAG GCCAGTCTTT 
GAGGATACCG GTCACAAGCC GGTCTCCTCT AATTCTCCCA AACCAGTTCC TGAGGAAGTG 
AAAGCGACCT ATTGGCCTCG ACCCCAGTTG GTCACAGGAT ACTGTACATA TCTTCCCTTT 
CGGGGATTTG ACATTACCCA GACTCCACCA GCCTTCATCA GTGTGTTACC GACTTTGCCC 
CAGTATAGCA TTTACACCCC TCTGGCCAGT GTTCCTACTG AGTATCACCT GCAAAGACCA 
ATACCAGTGG TGCCGTCTTT TGTAGCCAGT GACATAACGG AAGTGCATTT TGAGGCTCAT 
CATTTCAGTG CTGAGAATGC CCCTGGTACC CAGATTGCCT CCGAAACACA GATCCTTGAG 
GAATCTTTGG AAACACCAGT GAAGTCAGAG TGCAGCTATG ATGGTGCTGA TATAGTCCAG 
AGTGAGGCTA GCAGAAACGA TGACTACTGT GGCGATTCTG CCAAATGGGA AGTAAATCCA 
GAAAGCACCA ATGAAGTAAC AAATATTCCA TGTACACAAG TGGTTGCTGT ACAGGTGTCT 
TGGAATATAA CACATCAGGA AGTCAATACT GAGCCATATG GTCCTTTTGA GGCACAACAG 
GGGGGTATTT CACAGGTTGA AAAGGAGTAC CAGGTTTTAC AAGAGCAGCT TAAGGAAGCA 
TGTGAAAATT ATGAACACAG CAATGTCAAG GGCTCAGAAG AAACCAAGGA CTTGGAAGAA 
AAGTTGAAAA GGAATGTTGA AGAAAATAAG ATCTCAAAGA CAGAACTAGA TTGGTTCCTT 
GAAGATTTGA ACAAGGAAAT TAAAAAATGG GAACAGGAGA AAAAAGAAAT CCAAGAAAGA 
CTAAAGGCAC TGAAGAAGAA AATGAAAAAG GTGTTAAATG CCAGCAAAAT GTATACGCAG 
GAAAATGATG GAAAGGAAAA GGCACTTGAA TTACTTCTGG ATCAGTCCCT TGAAATCAGT 
AATACATTTA CAAATGAGAA AATGAAAATA GAAGAGAGTA TAAAGAAAGG GAAAGAGCAT 
TATGAAGAGA GTCTTCAAAG GGCTGTGGAT GCAGAGGTAT CTGTGCTTGA AAACTGGAAG 
GACATGGAAG TGTGTAAGTT ACAGAGCATG GAGTCACAAG CCGAAAGGTT TCTCAAGAAT 
CTGAAGCTAA TGAGCAGTGA TTCGGCAGCG TATTCTGACA TGGACTCAGA CATACATTCA 
TGGGAATCGT TTCTTTCTGA TGTTAGAGAA GAAATTGAGA AAGCAAAGTC TCAGTTTGAA 
GAACAAATTC AGGCAATTAA ATATGGTTCT CAGCTCAGTG AACTTCCTAA AGTGCAGATT 
TCTGAGCTTT CATTTCCTAC CTGTAACACG ATTCATCCTA AATTACTTCC TGAGTCTTCA 
GGCCAGGATG GTCAAGGTCC CTCCACTTCT ACAAGTCATG GGACCGGAGA CCAGACAGCA 
GCTCCCGAGG AGTCAAGTCT GGGCTCTGCA GCTGACAGCC CAGTGGAGTT GCCCTCCTGC 
AGTCAGAAAG CCCCCCAGCT GCCAGAGCAT GAGGGTGCTG GCCAGGCAAC TGGGCCAGAA 
CCAGGCCCAG TGGCTGATCA GAAGTTACCT GCTGCTCCAG AGCGTGCCAC TCATTCAAGC 
CAGTCTCCAA AGAAGCCGTT CAATAGCATT ATTGAGCACC TGTTGGTGGT GTTCCCGTGT 
TACAGCAGCA CGGAGCTTGC TGGTTTTATT AAAAAAGTTA GAAACAAAAA CAAGACCTCT 
CTGTCTGGAT TGAGTATTGA CGAAATTGTG CAAAGAGTGA CAGAACACGT CCTTGATGAA 
GAAAGAAAGA AAAAGGTGTC CTGCGCAAAT TCCTGTGAAA TGTGCCGAGA AGTATTCAAG 
TCCAAACACG TGCGTGAGCT GCAGTGTGGG CACAGGCTTC ACAAGGGGTG CTTCAAGCAG 
TGGCTGAAGA GCCAGCGCAC CTGCCCAGCC TGCCTGGGTC ACGACCTCCT GTCAGAGGAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010799977.1
CDS	12..5834
Translation	MADFPQGNFADYALLEDCPYVDDCIFAPEFMSSDYVRVTQLYCDGVGRQYKDYAQTEGNLEFDICTLWCSQPISVLQDYCDAIKVHIFWPLLFQRQQSSIITRMHSCVRTSGSHACEISLKKLQQLELMEDIVDLAKKVVNDTFFIGGLLRIGYKIENKILAMEEALNWVKYTGDVTILSKLGAVDNCWRMLSIFFTEYKYHITKVVTENCSLLEEFKTQSCAECLEQGELMKMKGNEEFSKERYDIAIIYYTRAIECRPENHLLYGNRALCFLRTGQFRNALGDGKRATILKNNWTKGHYRYCAALSMLGEYDWALQANIKAQKLCKNDPEGIKDLIQQHVKLQKQIEDLQGQTPASNPVKAFCESRAYIPSLSAPAFSTSLNFVETEKNFRKTNHEMANGGNQNPKVVDEALKVDDCDCHPEFVPPSSQPPKYKGKQKSRNNELEKFSPGSQETLPVDLKNILEKQFSKSSRAAHQDFANIMKMLRSLIQDGYTALLEQRCRSAAQAFTELLNGLDPQKIKQLNLAMINYVLVVYGLAISLLGIGQPEELSEAENQFKRMIEHYPNEGLDCLAYCGIGKVYLKKNRFLEALNHFEKARTLICRLPGVLTWPTSNVVIEETQPGKIKMLLEKFVEECRFPPVPDATCCYQKCHGYSKIQIYITDPDFKGFIRISCCQYCKIEFHMNCWKKLKTTTFNDKIDKDFLQGICLTPDCEGIISKIIIFSSGGQVKCEFEHKVIKEKVPPRPILKQKCSSLEKLRLKEDKKLKRKLQKKEAKKLAQERLEEDLRESNPPKTEEQRETINHVQSCQFLDDRILQCIKQYADKIKSGILNTSKLLKELLSWKVLTTEDYTTCFSSRNFLNEAVDYVIRHLIQEKNRVKTRVFLHVLSELKEVDPKLATWIQKLNSFGLDATGPFFSRYGAPLKELDFSIMTFLWNEKYGHKLGSIEGKQLDYFYEPTSLKEARCLIWLLEEHRDKFPALHNALDEFFDIMDSRCTVLRKQDSGDVPFNSTKIKNKGKKKKPKDSKPMLVGSGTPSVTPNNETVTSGEDHIKRSLSSAGPFVVPDHLRQDIEEFEALYEQHSNEYVVRNKKLWDINPKQKCSTLYDYFSQLLEEHGPLDMSNKMFSEEYEFFPEETQQILEKAGGLKAFLLGCPRFVVIDNCIALKKVALRLKKKRKKKNIKTKVEEISKTGEYLRVKLPLNPTAREFKPDVKSKPVSDLPSAPASEDMKPQPAPANSPQSARDTGKPKPVFDNSSRSVSEDEKPKGMSSDSPRPVFEDTGHKPVSSNSPKPVPEEVKATYWPRPQLVTGYCTYLPFRGFDITQTPPAFISVLPTLPQYSIYTPLASVPTEYHLQRPIPVVPSFVASDITEVHFEAHHFSAENAPGTQIASETQILEESLETPVKSECSYDGADIVQSEASRNDDYCGDSAKWEVNPESTNEVTNIPCTQVVAVQVSWNITHQEVNTEPYGPFEAQQGGISQVEKEYQVLQEQLKEACENYEHSNVKGSEETKDLEEKLKRNVEENKISKTELDWFLEDLNKEIKKWEQEKKEIQERLKALKKKMKKVLNASKMYTQENDGKEKALELLLDQSLEISNTFTNEKMKIEESIKKGKEHYEESLQRAVDAEVSVLENWKDMEVCKLQSMESQAERFLKNLKLMSSDSAAYSDMDSDIHSWESFLSDVREEIEKAKSQFEEQIQAIKYGSQLSELPKVQISELSFPTCNTIHPKLLPESSGQDGQGPSTSTSHGTGDQTAAPEESSLGSAADSPVELPSCSQKAPQLPEHEGAGQATGPEPGPVADQKLPAAPERATHSSQSPKKPFNSIIEHLLVVFPCYSSTELAGFIKKVRNKNKTSLSGLSIDEIVQRVTEHVLDEERKKKVSCANSCEMCREVFKSKHVRELQCGHRLHKGCFKQWLKSQRTCPACLGHDLLSEE

Target ORF information:

RefSeq Version	XM_010801675.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010801675.1

ORF Insert Sequence:

ATGGCCGATT TTCCCCAGGG AAATTTTGCA GATTATGCCT TGTTAGAAGA TTGCCCTTAT 
GTGGATGATT GTATCTTTGC CCCTGAGTTT ATGTCCAGTG ATTATGTTCG TGTGACTCAG 
CTTTACTGTG ATGGAGTGGG TAGACAGTAT AAAGATTATG CCCAAACTGA GGGAAACTTA 
GAATTTGACA TCTGCACTCT GTGGTGTAGT CAACCTATTT CTGTCCTGCA AGATTACTGT 
GATGCCATTA AAGTCCACAT CTTCTGGCCA CTTCTGTTTC AACGTCAGCA GAGCTCTATA 
ATAACACGGA TGCACTCCTG TGTAAGGACC AGCGGTTCTC ATGCTTGTGA GATAAGTTTG 
AAGAAATTAC AGCAGCTTGA ACTGATGGAA GATATTGTGG ATTTGGCAAA GAAAGTTGTG 
AATGATACAT TCTTTATCGG AGGCTTATTG AGAATTGGCT ATAAAATAGA GAATAAAATC 
TTGGCAATGG AAGAAGCTTT GAATTGGGTG AAATACACAG GCGATGTAAC AATTTTATCT 
AAATTAGGAG CAGTTGATAA TTGTTGGCGT ATGTTAAGTA TTTTCTTTAC TGAATACAAG 
TATCACATAA CTAAAGTTGT AACAGAAAAC TGCAGTTTGC TTGAAGAATT TAAAACTCAA 
AGTTGTGCGG AGTGTCTAGA GCAAGGAGAA CTAATGAAAA TGAAAGGAAA TGAAGAGTTT 
TCCAAAGAAA GATATGATAT AGCTATTATC TATTACACCA GAGCCATTGA ATGTAGGCCT 
GAAAATCACC TGCTTTATGG TAACCGAGCT CTTTGTTTTC TTCGTACTGG ACAATTCAGA 
AATGCACTTG GTGATGGAAA GAGAGCTACC ATTCTGAAGA ACAATTGGAC AAAGGGTCAT 
TATCGTTATT GTGCTGCTCT TTCTATGCTG GGGGAATATG ACTGGGCCCT GCAAGCAAAC 
ATAAAAGCTC AAAAACTCTG TAAAAATGAC CCTGAGGGAA TCAAGGATCT AATTCAGCAG 
CATGTAAAGT TACAAAAACA AATAGAAGAC CTGCAAGGTC AAACACCAGC TAGTAATCCA 
GTCAAAGCCT TCTGTGAAAG CAGGGCCTAC ATACCTAGTT TATCAGCACC TGCATTTAGT 
ACTTCACTTA ACTTTGTGGA AACTGAAAAA AATTTCAGAA AAACTAACCA CGAAATGGCA 
AACGGTGGTA ATCAGAATCC AAAGGTGGTA GATGAAGCTC TGAAAGTAGA TGATTGTGAC 
TGTCATCCTG AATTTGTACC GCCATCAAGT CAGCCTCCAA AATATAAAGG GAAACAAAAA 
TCTCGAAACA ATGAATTGGA GAAGTTCAGC CCTGGTTCAC AAGAAACTTT ACCAGTAGAT 
TTAAAAAACA TCTTGGAGAA ACAGTTTTCT AAATCTTCCA GAGCCGCACA CCAGGATTTT 
GCTAATATAA TGAAAATGCT GAGAAGTTTA ATTCAGGATG GCTACACGGC CTTGCTGGAG 
CAGCGCTGCC GCAGCGCCGC CCAAGCCTTC ACAGAGTTGC TCAACGGTTT AGATCCTCAA 
AAAATAAAGC AATTGAACCT GGCCATGATT AACTATGTGT TAGTAGTCTA CGGACTTGCC 
ATTTCACTCC TTGGAATAGG ACAGCCCGAG GAGTTATCTG AAGCTGAAAA CCAGTTCAAG 
AGGATGATTG AACACTACCC CAATGAGGGG CTTGATTGCT TGGCCTACTG TGGAATTGGA 
AAAGTGTATT TGAAAAAAAA CAGATTTTTA GAAGCTCTTA ATCATTTTGA GAAAGCAAGA 
ACCTTGATCT GCCGTCTTCC TGGAGTGTTA ACTTGGCCCA CAAGTAATGT GGTTATTGAG 
GAGACTCAGC CAGGGAAGAT AAAGATGCTG TTAGAGAAAT TTGTTGAAGA ATGCAGGTTC 
CCTCCAGTGC CGGATGCCAC TTGTTGCTAT CAGAAGTGCC ATGGATATTC CAAAATCCAG 
ATATATATAA CTGATCCAGA CTTTAAGGGT TTTATACGGA TCAGCTGTTG CCAGTATTGT 
AAAATAGAAT TTCACATGAA CTGCTGGAAG AAGTTAAAAA CAACAACCTT CAATGATAAA 
ATTGACAAGG ATTTTCTGCA AGGAATCTGT CTTACTCCTG ATTGTGAAGG AATCATTTCA 
AAGATTATCA TCTTCAGCAG TGGTGGTCAA GTTAAATGTG AATTTGAACA CAAGGTCATT 
AAAGAAAAGG TTCCTCCAAG ACCTATTCTG AAACAGAAAT GTTCTAGCCT AGAGAAGTTG 
AGGCTGAAAG AAGACAAAAA ATTGAAGCGA AAGCTCCAAA AAAAGGAAGC AAAAAAATTA 
GCACAAGAAA GACTGGAAGA GGACTTAAGG GAAAGTAACC CACCAAAAAC TGAAGAGCAG 
AGAGAAACTA TCAACCATGT CCAAAGTTGC CAGTTCCTTG ATGACAGAAT TCTGCAGTGC 
ATAAAGCAGT ACGCTGACAA GATTAAGTCC GGGATACTGA ATACTTCCAA GCTTCTCAAA 
GAATTGCTTT CTTGGAAGGT TTTAACCACA GAAGACTACA CAACGTGTTT TTCTAGCAGA 
AATTTCCTGA ATGAAGCAGT GGACTATGTC ATTCGTCATT TGATCCAAGA AAAGAATAGA 
GTAAAGACAA GGGTATTTCT GCATGTTTTG AGTGAGCTTA AAGAAGTGGA CCCAAAACTA 
GCCACCTGGA TCCAGAAACT GAATAGCTTT GGCTTAGATG CCACAGGACC TTTCTTTTCT 
CGTTATGGGG CTCCTCTTAA GGAGCTTGAT TTTAGCATCA TGACTTTTCT CTGGAATGAG 
AAATACGGTC ATAAGCTAGG CTCTATAGAA GGAAAGCAAC TTGATTATTT CTATGAGCCT 
ACATCACTGA AGGAAGCGCG GTGTTTAATA TGGCTGCTAG AAGAACACAG AGACAAGTTC 
CCAGCACTGC ATAATGCCTT GGACGAGTTC TTTGATATAA TGGACAGCCG CTGCACTGTA 
TTAAGGAAAC AGGACAGTGG TGACGTGCCA TTTAATTCTA CCAAGATAAA AAACAAAGGC 
AAGAAAAAGA AGCCAAAAGA TTCAAAGCCT ATGTTAGTTG GATCTGGAAC ACCTTCAGTC 
ACACCAAACA ATGAGACTGT CACTTCAGGT GAAGACCATA TCAAACGCAG TTTAAGTTCT 
GCGGGCCCCT TTGTAGTGCC TGATCACCTT CGGCAGGATA TAGAAGAATT TGAAGCTCTG 
TATGAACAGC ATAGTAATGA GTATGTTGTC CGTAATAAGA AGCTGTGGGA CATAAACCCA 
AAACAGAAAT GTTCAACTCT ATACGATTAT TTCTCTCAGT TGTTGGAGGA ACATGGTCCC 
TTGGACATGA GTAACAAAAT GTTTTCTGAA GAATATGAGT TCTTCCCAGA AGAAACTCAA 
CAGATACTAG AAAAAGCAGG AGGCTTGAAA GCGTTTCTCC TGGGATGTCC TCGTTTTGTT 
GTGATTGATA ACTGTATCGC ATTGAAGAAA GTTGCATTAC GGCTCAAGAA AAAGAGAAAG 
AAGAAAAACA TTAAAACAAA AGTAGAAGAA ATTTCAAAAA CAGGAGAGTA TTTACGAGTT 
AAACTACCAC TGAATCCAAC TGCTAGGGAA TTTAAACCAG ATGTAAAGTC CAAACCAGTG 
TCTGACTTAC CTTCAGCACC AGCTTCTGAG GACATGAAGC CTCAGCCTGC ACCTGCTAAT 
TCTCCCCAAT CAGCTCGTGA CACTGGGAAG CCAAAACCAG TTTTCGATAA TTCTTCCAGA 
TCAGTTTCTG AGGATGAGAA GCCCAAAGGG ATGTCTTCTG ATTCTCCCAG GCCAGTCTTT 
GAGGATACCG GTCACAAGCC GGTCTCCTCT AATTCTCCCA AACCAGTTCC TGAGGAAGTG 
AAAGCGACCT ATTGGCCTCG ACCCCAGTTG GTCACAGGAT ACTGTACATA TCTTCCCTTT 
CGGGGATTTG ACATTACCCA GACTCCACCA GCCTTCATCA GTGTGTTACC GACTTTGCCC 
CAGTATAGCA TTTACACCCC TCTGGCCAGT GTTCCTACTG AGTATCACCT GCAAAGACCA 
ATACCAGTGG TGCCGTCTTT TGTAGCCAGT GACATAACGG AAGTGCATTT TGAGGCTCAT 
CATTTCAGTG CTGAGAATGC CCCTGGTACC CAGATTGCCT CCGAAACACA GATCCTTGAG 
GAATCTTTGG AAACACCAGT GAAGTCAGAG TGCAGCTATG ATGGTGCTGA TATAGTCCAG 
AGTGAGGCTA GCAGAAACGA TGACTACTGT GGCGATTCTG CCAAATGGGA AGTAAATCCA 
GAAAGCACCA ATGAAGTAAC AAATATTCCA TGTACACAAG TGGTTGCTGT ACAGGTGTCT 
TGGAATATAA CACATCAGGA AGTCAATACT GAGCCATATG GTCCTTTTGA GGCACAACAG 
GGGGGTATTT CACAGGTTGA AAAGGAGTAC CAGGTTTTAC AAGAGCAGCT TAAGGAAGCA 
TGTGAAAATT ATGAACACAG CAATGTCAAG GGCTCAGAAG AAACCAAGGA CTTGGAAGAA 
AAGTTGAAAA GGAATGTTGA AGAAAATAAG ATCTCAAAGA CAGAACTAGA TTGGTTCCTT 
GAAGATTTGA ACAAGGAAAT TAAAAAATGG GAACAGGAGA AAAAAGAAAT CCAAGAAAGA 
CTAAAGGCAC TGAAGAAGAA AATGAAAAAG GTGTTAAATG CCAGCAAAAT GTATACGCAG 
GAAAATGATG GAAAGGAAAA GGCACTTGAA TTACTTCTGG ATCAGTCCCT TGAAATCAGT 
AATACATTTA CAAATGAGAA AATGAAAATA GAAGAGAGTA TAAAGAAAGG GAAAGAGCAT 
TATGAAGAGA GTCTTCAAAG GGCTGTGGAT GCAGAGGTAT CTGTGCTTGA AAACTGGAAG 
GACATGGAAG TGTGTAAGTT ACAGAGCATG GAGTCACAAG CCGAAAGGTT TCTCAAGAAT 
CTGAAGCTAA TGAGCAGTGA TTCGGCAGCG TATTCTGACA TGGACTCAGA CATACATTCA 
TGGGAATCGT TTCTTTCTGA TGTTAGAGAA GAAATTGAGA AAGCAAAGTC TCAGTTTGAA 
GAACAAATTC AGGCAATTAA ATATGGTTCT CAGCTCAGTG AACTTCCTAA AGTGCAGATT 
TCTGAGCTTT CATTTCCTAC CTGTAACACG ATTCATCCTA AATTACTTCC TGAGTCTTCA 
GGCCAGGATG GTCAAGGTCC CTCCACTTCT ACAAGTCATG GGACCGGAGA CCAGACAGCA 
GCTCCCGAGG AGTCAAGTCT GGGCTCTGCA GCTGACAGCC CAGTGGAGTT GCCCTCCTGC 
AGTCAGAAAG CCCCCCAGCT GCCAGAGCAT GAGGGTGCTG GCCAGGCAAC TGGGCCAGAA 
CCAGGCCCAG TGGCTGATCA GAAGTTACCT GCTGCTCCAG AGCGTGCCAC TCATTCAAGC 
CAGTCTCCAA AGAAGCCGTT CAATAGCATT ATTGAGCACC TGTTGGTGGT GTTCCCGTGT 
TACAGCAGCA CGGAGCTTGC TGGTTTTATT AAAAAAGTTA GAAACAAAAA CAAGACCTCT 
CTGTCTGGAT TGAGTATTGA CGAAATTGTG CAAAGAGTGA CAGAACACGT CCTTGATGAA 
GAAAGAAAGA AAAAGGTGTC CTGCGCAAAT TCCTGTGAAA TGTGCCGAGA AGTATTCAAG 
TCCAAACACG TGCGTGAGCT GCAGTGTGGG CACAGGCTTC ACAAGGGGTG CTTCAAGCAG 
TGGCTGAAGA GCCAGCGCAC CTGCCCAGCC TGCCTGGGTC ACGACCTCCT GTCAGAGGAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_010801677.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010799979.1 Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X6, mRNA.

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CloneID	OBa46604
Clone ID Related Accession (Same CDS sequence)	XM_010801677.2
Accession Version	XM_010801677.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5649bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TTC3 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103826.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010801677.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTCCT GTGTAAGGAC CAGCGGTTCT CATGCTTGTG AGATAAGTTT GAAGAAATTA 
CAGCAGCTTG AACTGATGGA AGATATTGTG GATTTGGCAA AGAAAGTTGT GAATGATACA 
TTCTTTATCG GAGGCTTATT GAGAATTGGC TATAAAATAG AGAATAAAAT CTTGGCAATG 
GAAGAAGCTT TGAATTGGGT GAAATACACA GGCGATGTAA CAATTTTATC TAAATTAGGA 
GCAGTTGATA ATTGTTGGCG TATGTTAAGT ATTTTCTTTA CTGAATACAA GTATCACATA 
ACTAAAGTTG TAACAGAAAA CTGCAGTTTG CTTGAAGAAT TTAAAACTCA AAGTTGTGCG 
GAGTGTCTAG AGCAAGGAGA ACTAATGAAA ATGAAAGGAA ATGAAGAGTT TTCCAAAGAA 
AGATATGATA TAGCTATTAT CTATTACACC AGAGCCATTG AATGTAGGCC TGAAAATCAC 
CTGCTTTATG GTAACCGAGC TCTTTGTTTT CTTCGTACTG GACAATTCAG AAATGCACTT 
GGTGATGGAA AGAGAGCTAC CATTCTGAAG AACAATTGGA CAAAGGGTCA TTATCGTTAT 
TGTGCTGCTC TTTCTATGCT GGGGGAATAT GACTGGGCCC TGCAAGCAAA CATAAAAGCT 
CAAAAACTCT GTAAAAATGA CCCTGAGGGA ATCAAGGATC TAATTCAGCA GCATGTAAAG 
TTACAAAAAC AAATAGAAGA CCTGCAAGGT CAAACACCAG CTAGTAATCC AGTCAAAGCC 
TTCTGTGAAA GCAGGGCCTA CATACCTAGT TTATCAGCAC CTGCATTTAG TACTTCACTT 
AACTTTGTGG AAACTGAAAA AAATTTCAGA AAAACTAACC ACGAAATGGC AAACGGTGGT 
AATCAGAATC CAAAGGTGGT AGATGAAGCT CTGAAAGTAG ATGATTGTGA CTGTCATCCT 
GAATTTGTAC CGCCATCAAG TCAGCCTCCA AAATATAAAG GGAAACAAAA ATCTCGAAAC 
AATGAATTGG AGAAGTTCAG CCCTGGTTCA CAAGAAACTT TACCAGTAGA TTTAAAAAAC 
ATCTTGGAGA AACAGTTTTC TAAATCTTCC AGAGCCGCAC ACCAGGATTT TGCTAATATA 
ATGAAAATGC TGAGAAGTTT AATTCAGGAT GGCTACACGG CCTTGCTGGA GCAGCGCTGC 
CGCAGCGCCG CCCAAGCCTT CACAGAGTTG CTCAACGGTT TAGATCCTCA AAAAATAAAG 
CAATTGAACC TGGCCATGAT TAACTATGTG TTAGTAGTCT ACGGACTTGC CATTTCACTC 
CTTGGAATAG GACAGCCCGA GGAGTTATCT GAAGCTGAAA ACCAGTTCAA GAGGATGATT 
GAACACTACC CCAATGAGGG GCTTGATTGC TTGGCCTACT GTGGAATTGG AAAAGTGTAT 
TTGAAAAAAA ACAGATTTTT AGAAGCTCTT AATCATTTTG AGAAAGCAAG AACCTTGATC 
TGCCGTCTTC CTGGAGTGTT AACTTGGCCC ACAAGTAATG TGGTTATTGA GGAGACTCAG 
CCAGGGAAGA TAAAGATGCT GTTAGAGAAA TTTGTTGAAG AATGCAGGTT CCCTCCAGTG 
CCGGATGCCA CTTGTTGCTA TCAGAAGTGC CATGGATATT CCAAAATCCA GATATATATA 
ACTGATCCAG ACTTTAAGGG TTTTATACGG ATCAGCTGTT GCCAGTATTG TAAAATAGAA 
TTTCACATGA ACTGCTGGAA GAAGTTAAAA ACAACAACCT TCAATGATAA AATTGACAAG 
GATTTTCTGC AAGGAATCTG TCTTACTCCT GATTGTGAAG GAATCATTTC AAAGATTATC 
ATCTTCAGCA GTGGTGGTCA AGTTAAATGT GAATTTGAAC ACAAGGTCAT TAAAGAAAAG 
GTTCCTCCAA GACCTATTCT GAAACAGAAA TGTTCTAGCC TAGAGAAGTT GAGGCTGAAA 
GAAGACAAAA AATTGAAGCG AAAGCTCCAA AAAAAGGAAG CAAAAAAATT AGCACAAGAA 
AGACTGGAAG AGGACTTAAG GGAAAGTAAC CCACCAAAAA CTGAAGAGCA GAGAGAAACT 
ATCAACCATG TCCAAAGTTG CCAGTTCCTT GATGACAGAA TTCTGCAGTG CATAAAGCAG 
TACGCTGACA AGATTAAGTC CGGGATACTG AATACTTCCA AGCTTCTCAA AGAATTGCTT 
TCTTGGAAGG TTTTAACCAC AGAAGACTAC ACAACGTGTT TTTCTAGCAG AAATTTCCTG 
AATGAAGCAG TGGACTATGT CATTCGTCAT TTGATCCAAG AAAAGAATAG AGTAAAGACA 
AGGGTATTTC TGCATGTTTT GAGTGAGCTT AAAGAAGTGG ACCCAAAACT AGCCACCTGG 
ATCCAGAAAC TGAATAGCTT TGGCTTAGAT GCCACAGGAC CTTTCTTTTC TCGTTATGGG 
GCTCCTCTTA AGGAGCTTGA TTTTAGCATC ATGACTTTTC TCTGGAATGA GAAATACGGT 
CATAAGCTAG GCTCTATAGA AGGAAAGCAA CTTGATTATT TCTATGAGCC TACATCACTG 
AAGGAAGCGC GGTGTTTAAT ATGGCTGCTA GAAGAACACA GAGACAAGTT CCCAGCACTG 
CATAATGCCT TGGACGAGTT CTTTGATATA ATGGACAGCC GCTGCACTGT ATTAAGGAAA 
CAGGACAGTG GTGACGTGCC ATTTAATTCT ACCAAGATAA AAAACAAAGG CAAGAAAAAG 
AAGCCAAAAG ATTCAAAGCC TATGTTAGTT GGATCTGGAA CACCTTCAGT CACACCAAAC 
AATGAGACTG TCACTTCAGG TGAAGACCAT ATCAAACGCA GTTTAAGTTC TGCGGGCCCC 
TTTGTAGTGC CTGATCACCT TCGGCAGGAT ATAGAAGAAT TTGAAGCTCT GTATGAACAG 
CATAGTAATG AGTATGTTGT CCGTAATAAG AAGCTGTGGG ACATAAACCC AAAACAGAAA 
TGTTCAACTC TATACGATTA TTTCTCTCAG TTGTTGGAGG AACATGGTCC CTTGGACATG 
AGTAACAAAA TGTTTTCTGA AGAATATGAG TTCTTCCCAG AAGAAACTCA ACAGATACTA 
GAAAAAGCAG GAGGCTTGAA AGCGTTTCTC CTGGGATGTC CTCGTTTTGT TGTGATTGAT 
AACTGTATCG CATTGAAGAA AGTTGCATTA CGGCTCAAGA AAAAGAGAAA GAAGAAAAAC 
ATTAAAACAA AAGTAGAAGA AATTTCAAAA ACAGGAGAGT ATTTACGAGT TAAACTACCA 
CTGAATCCAA CTGCTAGGGA ATTTAAACCA GATGTAAAGT CCAAACCAGT GTCTGACTTA 
CCTTCAGCAC CAGCTTCTGA GGACATGAAG CCTCAGCCTG CACCTGCTAA TTCTCCCCAA 
TCAGCTCGTG ACACTGGGAA GCCAAAACCA GTTTTCGATA ATTCTTCCAG ATCAGTTTCT 
GAGGATGAGA AGCCCAAAGG GATGTCTTCT GATTCTCCCA GGCCAGTCTT TGAGGATACC 
GGTCACAAGC CGGTCTCCTC TAATTCTCCC AAACCAGTTC CTGAGGAAGT GAAAGCGACC 
TATTGGCCTC GACCCCAGTT GGTCACAGGA TACTGTACAT ATCTTCCCTT TCGGGGATTT 
GACATTACCC AGACTCCACC AGCCTTCATC AGTGTGTTAC CGACTTTGCC CCAGTATAGC 
ATTTACACCC CTCTGGCCAG TGTTCCTACT GAGTATCACC TGCAAAGACC AATACCAGTG 
GTGCCGTCTT TTGTAGCCAG TGACATAACG GAAGTGCATT TTGAGGCTCA TCATTTCAGT 
GCTGAGAATG CCCCTGGTAC CCAGATTGCC TCCGAAACAC AGATCCTTGA GGAATCTTTG 
GAAACACCAG TGAAGTCAGA GTGCAGCTAT GATGGTGCTG ATATAGTCCA GAGTGAGGCT 
AGCAGAAACG ATGACTACTG TGGCGATTCT GCCAAATGGG AAGTAAATCC AGAAAGCACC 
AATGAAGTAA CAAATATTCC ATGTACACAA GTGGTTGCTG TACAGGTGTC TTGGAATATA 
ACACATCAGG AAGTCAATAC TGAGCCATAT GGTCCTTTTG AGGCACAACA GGGGGGTATT 
TCACAGGTTG AAAAGGAGTA CCAGGTTTTA CAAGAGCAGC TTAAGGAAGC ATGTGAAAAT 
TATGAACACA GCAATGTCAA GGGCTCAGAA GAAACCAAGG ACTTGGAAGA AAAGTTGAAA 
AGGAATGTTG AAGAAAATAA GATCTCAAAG ACAGAACTAG ATTGGTTCCT TGAAGATTTG 
AACAAGGAAA TTAAAAAATG GGAACAGGAG AAAAAAGAAA TCCAAGAAAG ACTAAAGGCA 
CTGAAGAAGA AAATGAAAAA GGTGTTAAAT GCCAGCAAAA TGTATACGCA GGAAAATGAT 
GGAAAGGAAA AGGCACTTGA ATTACTTCTG GATCAGTCCC TTGAAATCAG TAATACATTT 
ACAAATGAGA AAATGAAAAT AGAAGAGAGT ATAAAGAAAG GGAAAGAGCA TTATGAAGAG 
AGTCTTCAAA GGGCTGTGGA TGCAGAGGTA TCTGTGCTTG AAAACTGGAA GGACATGGAA 
GTGTGTAAGT TACAGAGCAT GGAGTCACAA GCCGAAAGGT TTCTCAAGAA TCTGAAGCTA 
ATGAGCAGTG ATTCGGCAGC GTATTCTGAC ATGGACTCAG ACATACATTC ATGGGAATCG 
TTTCTTTCTG ATGTTAGAGA AGAAATTGAG AAAGCAAAGT CTCAGTTTGA AGAACAAATT 
CAGGCAATTA AATATGGTTC TCAGCTCAGT GAACTTCCTA AAGTGCAGAT TTCTGAGCTT 
TCATTTCCTA CCTGTAACAC GATTCATCCT AAATTACTTC CTGAGTCTTC AGGCCAGGAT 
GGTCAAGGTC CCTCCACTTC TACAAGTCAT GGGACCGGAG ACCAGACAGC AGCTCCCGAG 
GAGTCAAGTC TGGGCTCTGC AGCTGACAGC CCAGTGGAGT TGCCCTCCTG CAGTCAGAAA 
GCCCCCCAGC TGCCAGAGCA TGAGGGTGCT GGCCAGGCAA CTGGGCCAGA ACCAGGCCCA 
GTGGCTGATC AGAAGTTACC TGCTGCTCCA GAGCGTGCCA CTCATTCAAG CCAGTCTCCA 
AAGAAGCCGT TCAATAGCAT TATTGAGCAC CTGTTGGTGG TGTTCCCGTG TTACAGCAGC 
ACGGAGCTTG CTGGTTTTAT TAAAAAAGTT AGAAACAAAA ACAAGACCTC TCTGTCTGGA 
TTGAGTATTG ACGAAATTGT GCAAAGAGTG ACAGAACACG TCCTTGATGA AGAAAGAAAG 
AAAAAGCCAA GTCCAGGAAA GGGCTGGAGG CTGTCTGCAC CCAGCTCTGC TGTTGCTGTG 
CCCAGGGCTG CCCAGGACCC GCCCTCCATG CCTGTCGGAG CATCGCCCGA AACCAGGGGG 
CCGAGGACCG AGGACGTCCC TGTGTCCTGC GCAAATTCCT GTGAAATGTG CCGAGAAGTA 
TTCAAGTCCA AACACGTGCG TGAGCTGCAG TGTGGGCACA GGCTTCACAA GGGGTGCTTC 
AAGCAGTGGC TGAAGAGCCA GCGCACCTGC CCAGCCTGCC TGGGTCACGA CCTCCTGTCA 
GAGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010799979.1
CDS	232..5880
Translation	MHSCVRTSGSHACEISLKKLQQLELMEDIVDLAKKVVNDTFFIGGLLRIGYKIENKILAMEEALNWVKYTGDVTILSKLGAVDNCWRMLSIFFTEYKYHITKVVTENCSLLEEFKTQSCAECLEQGELMKMKGNEEFSKERYDIAIIYYTRAIECRPENHLLYGNRALCFLRTGQFRNALGDGKRATILKNNWTKGHYRYCAALSMLGEYDWALQANIKAQKLCKNDPEGIKDLIQQHVKLQKQIEDLQGQTPASNPVKAFCESRAYIPSLSAPAFSTSLNFVETEKNFRKTNHEMANGGNQNPKVVDEALKVDDCDCHPEFVPPSSQPPKYKGKQKSRNNELEKFSPGSQETLPVDLKNILEKQFSKSSRAAHQDFANIMKMLRSLIQDGYTALLEQRCRSAAQAFTELLNGLDPQKIKQLNLAMINYVLVVYGLAISLLGIGQPEELSEAENQFKRMIEHYPNEGLDCLAYCGIGKVYLKKNRFLEALNHFEKARTLICRLPGVLTWPTSNVVIEETQPGKIKMLLEKFVEECRFPPVPDATCCYQKCHGYSKIQIYITDPDFKGFIRISCCQYCKIEFHMNCWKKLKTTTFNDKIDKDFLQGICLTPDCEGIISKIIIFSSGGQVKCEFEHKVIKEKVPPRPILKQKCSSLEKLRLKEDKKLKRKLQKKEAKKLAQERLEEDLRESNPPKTEEQRETINHVQSCQFLDDRILQCIKQYADKIKSGILNTSKLLKELLSWKVLTTEDYTTCFSSRNFLNEAVDYVIRHLIQEKNRVKTRVFLHVLSELKEVDPKLATWIQKLNSFGLDATGPFFSRYGAPLKELDFSIMTFLWNEKYGHKLGSIEGKQLDYFYEPTSLKEARCLIWLLEEHRDKFPALHNALDEFFDIMDSRCTVLRKQDSGDVPFNSTKIKNKGKKKKPKDSKPMLVGSGTPSVTPNNETVTSGEDHIKRSLSSAGPFVVPDHLRQDIEEFEALYEQHSNEYVVRNKKLWDINPKQKCSTLYDYFSQLLEEHGPLDMSNKMFSEEYEFFPEETQQILEKAGGLKAFLLGCPRFVVIDNCIALKKVALRLKKKRKKKNIKTKVEEISKTGEYLRVKLPLNPTAREFKPDVKSKPVSDLPSAPASEDMKPQPAPANSPQSARDTGKPKPVFDNSSRSVSEDEKPKGMSSDSPRPVFEDTGHKPVSSNSPKPVPEEVKATYWPRPQLVTGYCTYLPFRGFDITQTPPAFISVLPTLPQYSIYTPLASVPTEYHLQRPIPVVPSFVASDITEVHFEAHHFSAENAPGTQIASETQILEESLETPVKSECSYDGADIVQSEASRNDDYCGDSAKWEVNPESTNEVTNIPCTQVVAVQVSWNITHQEVNTEPYGPFEAQQGGISQVEKEYQVLQEQLKEACENYEHSNVKGSEETKDLEEKLKRNVEENKISKTELDWFLEDLNKEIKKWEQEKKEIQERLKALKKKMKKVLNASKMYTQENDGKEKALELLLDQSLEISNTFTNEKMKIEESIKKGKEHYEESLQRAVDAEVSVLENWKDMEVCKLQSMESQAERFLKNLKLMSSDSAAYSDMDSDIHSWESFLSDVREEIEKAKSQFEEQIQAIKYGSQLSELPKVQISELSFPTCNTIHPKLLPESSGQDGQGPSTSTSHGTGDQTAAPEESSLGSAADSPVELPSCSQKAPQLPEHEGAGQATGPEPGPVADQKLPAAPERATHSSQSPKKPFNSIIEHLLVVFPCYSSTELAGFIKKVRNKNKTSLSGLSIDEIVQRVTEHVLDEERKKKPSPGKGWRLSAPSSAVAVPRAAQDPPSMPVGASPETRGPRTEDVPVSCANSCEMCREVFKSKHVRELQCGHRLHKGCFKQWLKSQRTCPACLGHDLLSEE

Target ORF information:

RefSeq Version	XM_010801677.2
Organism	Bos taurus(cattle)
Definition	Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010801677.2

ORF Insert Sequence:

ATGCACTCCT GTGTAAGGAC CAGCGGTTCT CATGCTTGTG AGATAAGTTT GAAGAAATTA 
CAGCAGCTTG AACTGATGGA AGATATTGTG GATTTGGCAA AGAAAGTTGT GAATGATACA 
TTCTTTATCG GAGGCTTATT GAGAATTGGC TATAAAATAG AGAATAAAAT CTTGGCAATG 
GAAGAAGCTT TGAATTGGGT GAAATACACA GGCGATGTAA CAATTTTATC TAAATTAGGA 
GCAGTTGATA ATTGTTGGCG TATGTTAAGT ATTTTCTTTA CTGAATACAA GTATCACATA 
ACTAAAGTTG TAACAGAAAA CTGCAGTTTG CTTGAAGAAT TTAAAACTCA AAGTTGTGCG 
GAGTGTCTAG AGCAAGGAGA ACTAATGAAA ATGAAAGGAA ATGAAGAGTT TTCCAAAGAA 
AGATATGATA TAGCTATTAT CTATTACACC AGAGCCATTG AATGTAGGCC TGAAAATCAC 
CTGCTTTATG GTAACCGAGC TCTTTGTTTT CTTCGTACTG GACAATTCAG AAATGCACTT 
GGTGATGGAA AGAGAGCTAC CATTCTGAAG AACAATTGGA CAAAGGGTCA TTATCGTTAT 
TGTGCTGCTC TTTCTATGCT GGGGGAATAT GACTGGGCCC TGCAAGCAAA CATAAAAGCT 
CAAAAACTCT GTAAAAATGA CCCTGAGGGA ATCAAGGATC TAATTCAGCA GCATGTAAAG 
TTACAAAAAC AAATAGAAGA CCTGCAAGGT CAAACACCAG CTAGTAATCC AGTCAAAGCC 
TTCTGTGAAA GCAGGGCCTA CATACCTAGT TTATCAGCAC CTGCATTTAG TACTTCACTT 
AACTTTGTGG AAACTGAAAA AAATTTCAGA AAAACTAACC ACGAAATGGC AAACGGTGGT 
AATCAGAATC CAAAGGTGGT AGATGAAGCT CTGAAAGTAG ATGATTGTGA CTGTCATCCT 
GAATTTGTAC CGCCATCAAG TCAGCCTCCA AAATATAAAG GGAAACAAAA ATCTCGAAAC 
AATGAATTGG AGAAGTTCAG CCCTGGTTCA CAAGAAACTT TACCAGTAGA TTTAAAAAAC 
ATCTTGGAGA AACAGTTTTC TAAATCTTCC AGAGCCGCAC ACCAGGATTT TGCTAATATA 
ATGAAAATGC TGAGAAGTTT AATTCAGGAT GGCTACACGG CCTTGCTGGA GCAGCGCTGC 
CGCAGCGCCG CCCAAGCCTT CACAGAGTTG CTCAACGGTT TAGATCCTCA AAAAATAAAG 
CAATTGAACC TGGCCATGAT TAACTATGTG TTAGTAGTCT ACGGACTTGC CATTTCACTC 
CTTGGAATAG GACAGCCCGA GGAGTTATCT GAAGCTGAAA ACCAGTTCAA GAGGATGATT 
GAACACTACC CCAATGAGGG GCTTGATTGC TTGGCCTACT GTGGAATTGG AAAAGTGTAT 
TTGAAAAAAA ACAGATTTTT AGAAGCTCTT AATCATTTTG AGAAAGCAAG AACCTTGATC 
TGCCGTCTTC CTGGAGTGTT AACTTGGCCC ACAAGTAATG TGGTTATTGA GGAGACTCAG 
CCAGGGAAGA TAAAGATGCT GTTAGAGAAA TTTGTTGAAG AATGCAGGTT CCCTCCAGTG 
CCGGATGCCA CTTGTTGCTA TCAGAAGTGC CATGGATATT CCAAAATCCA GATATATATA 
ACTGATCCAG ACTTTAAGGG TTTTATACGG ATCAGCTGTT GCCAGTATTG TAAAATAGAA 
TTTCACATGA ACTGCTGGAA GAAGTTAAAA ACAACAACCT TCAATGATAA AATTGACAAG 
GATTTTCTGC AAGGAATCTG TCTTACTCCT GATTGTGAAG GAATCATTTC AAAGATTATC 
ATCTTCAGCA GTGGTGGTCA AGTTAAATGT GAATTTGAAC ACAAGGTCAT TAAAGAAAAG 
GTTCCTCCAA GACCTATTCT GAAACAGAAA TGTTCTAGCC TAGAGAAGTT GAGGCTGAAA 
GAAGACAAAA AATTGAAGCG AAAGCTCCAA AAAAAGGAAG CAAAAAAATT AGCACAAGAA 
AGACTGGAAG AGGACTTAAG GGAAAGTAAC CCACCAAAAA CTGAAGAGCA GAGAGAAACT 
ATCAACCATG TCCAAAGTTG CCAGTTCCTT GATGACAGAA TTCTGCAGTG CATAAAGCAG 
TACGCTGACA AGATTAAGTC CGGGATACTG AATACTTCCA AGCTTCTCAA AGAATTGCTT 
TCTTGGAAGG TTTTAACCAC AGAAGACTAC ACAACGTGTT TTTCTAGCAG AAATTTCCTG 
AATGAAGCAG TGGACTATGT CATTCGTCAT TTGATCCAAG AAAAGAATAG AGTAAAGACA 
AGGGTATTTC TGCATGTTTT GAGTGAGCTT AAAGAAGTGG ACCCAAAACT AGCCACCTGG 
ATCCAGAAAC TGAATAGCTT TGGCTTAGAT GCCACAGGAC CTTTCTTTTC TCGTTATGGG 
GCTCCTCTTA AGGAGCTTGA TTTTAGCATC ATGACTTTTC TCTGGAATGA GAAATACGGT 
CATAAGCTAG GCTCTATAGA AGGAAAGCAA CTTGATTATT TCTATGAGCC TACATCACTG 
AAGGAAGCGC GGTGTTTAAT ATGGCTGCTA GAAGAACACA GAGACAAGTT CCCAGCACTG 
CATAATGCCT TGGACGAGTT CTTTGATATA ATGGACAGCC GCTGCACTGT ATTAAGGAAA 
CAGGACAGTG GTGACGTGCC ATTTAATTCT ACCAAGATAA AAAACAAAGG CAAGAAAAAG 
AAGCCAAAAG ATTCAAAGCC TATGTTAGTT GGATCTGGAA CACCTTCAGT CACACCAAAC 
AATGAGACTG TCACTTCAGG TGAAGACCAT ATCAAACGCA GTTTAAGTTC TGCGGGCCCC 
TTTGTAGTGC CTGATCACCT TCGGCAGGAT ATAGAAGAAT TTGAAGCTCT GTATGAACAG 
CATAGTAATG AGTATGTTGT CCGTAATAAG AAGCTGTGGG ACATAAACCC AAAACAGAAA 
TGTTCAACTC TATACGATTA TTTCTCTCAG TTGTTGGAGG AACATGGTCC CTTGGACATG 
AGTAACAAAA TGTTTTCTGA AGAATATGAG TTCTTCCCAG AAGAAACTCA ACAGATACTA 
GAAAAAGCAG GAGGCTTGAA AGCGTTTCTC CTGGGATGTC CTCGTTTTGT TGTGATTGAT 
AACTGTATCG CATTGAAGAA AGTTGCATTA CGGCTCAAGA AAAAGAGAAA GAAGAAAAAC 
ATTAAAACAA AAGTAGAAGA AATTTCAAAA ACAGGAGAGT ATTTACGAGT TAAACTACCA 
CTGAATCCAA CTGCTAGGGA ATTTAAACCA GATGTAAAGT CCAAACCAGT GTCTGACTTA 
CCTTCAGCAC CAGCTTCTGA GGACATGAAG CCTCAGCCTG CACCTGCTAA TTCTCCCCAA 
TCAGCTCGTG ACACTGGGAA GCCAAAACCA GTTTTCGATA ATTCTTCCAG ATCAGTTTCT 
GAGGATGAGA AGCCCAAAGG GATGTCTTCT GATTCTCCCA GGCCAGTCTT TGAGGATACC 
GGTCACAAGC CGGTCTCCTC TAATTCTCCC AAACCAGTTC CTGAGGAAGT GAAAGCGACC 
TATTGGCCTC GACCCCAGTT GGTCACAGGA TACTGTACAT ATCTTCCCTT TCGGGGATTT 
GACATTACCC AGACTCCACC AGCCTTCATC AGTGTGTTAC CGACTTTGCC CCAGTATAGC 
ATTTACACCC CTCTGGCCAG TGTTCCTACT GAGTATCACC TGCAAAGACC AATACCAGTG 
GTGCCGTCTT TTGTAGCCAG TGACATAACG GAAGTGCATT TTGAGGCTCA TCATTTCAGT 
GCTGAGAATG CCCCTGGTAC CCAGATTGCC TCCGAAACAC AGATCCTTGA GGAATCTTTG 
GAAACACCAG TGAAGTCAGA GTGCAGCTAT GATGGTGCTG ATATAGTCCA GAGTGAGGCT 
AGCAGAAACG ATGACTACTG TGGCGATTCT GCCAAATGGG AAGTAAATCC AGAAAGCACC 
AATGAAGTAA CAAATATTCC ATGTACACAA GTGGTTGCTG TACAGGTGTC TTGGAATATA 
ACACATCAGG AAGTCAATAC TGAGCCATAT GGTCCTTTTG AGGCACAACA GGGGGGTATT 
TCACAGGTTG AAAAGGAGTA CCAGGTTTTA CAAGAGCAGC TTAAGGAAGC ATGTGAAAAT 
TATGAACACA GCAATGTCAA GGGCTCAGAA GAAACCAAGG ACTTGGAAGA AAAGTTGAAA 
AGGAATGTTG AAGAAAATAA GATCTCAAAG ACAGAACTAG ATTGGTTCCT TGAAGATTTG 
AACAAGGAAA TTAAAAAATG GGAACAGGAG AAAAAAGAAA TCCAAGAAAG ACTAAAGGCA 
CTGAAGAAGA AAATGAAAAA GGTGTTAAAT GCCAGCAAAA TGTATACGCA GGAAAATGAT 
GGAAAGGAAA AGGCACTTGA ATTACTTCTG GATCAGTCCC TTGAAATCAG TAATACATTT 
ACAAATGAGA AAATGAAAAT AGAAGAGAGT ATAAAGAAAG GGAAAGAGCA TTATGAAGAG 
AGTCTTCAAA GGGCTGTGGA TGCAGAGGTA TCTGTGCTTG AAAACTGGAA GGACATGGAA 
GTGTGTAAGT TACAGAGCAT GGAGTCACAA GCCGAAAGGT TTCTCAAGAA TCTGAAGCTA 
ATGAGCAGTG ATTCGGCAGC GTATTCTGAC ATGGACTCAG ACATACATTC ATGGGAATCG 
TTTCTTTCTG ATGTTAGAGA AGAAATTGAG AAAGCAAAGT CTCAGTTTGA AGAACAAATT 
CAGGCAATTA AATATGGTTC TCAGCTCAGT GAACTTCCTA AAGTGCAGAT TTCTGAGCTT 
TCATTTCCTA CCTGTAACAC GATTCATCCT AAATTACTTC CTGAGTCTTC AGGCCAGGAT 
GGTCAAGGTC CCTCCACTTC TACAAGTCAT GGGACCGGAG ACCAGACAGC AGCTCCCGAG 
GAGTCAAGTC TGGGCTCTGC AGCTGACAGC CCAGTGGAGT TGCCCTCCTG CAGTCAGAAA 
GCCCCCCAGC TGCCAGAGCA TGAGGGTGCT GGCCAGGCAA CTGGGCCAGA ACCAGGCCCA 
GTGGCTGATC AGAAGTTACC TGCTGCTCCA GAGCGTGCCA CTCATTCAAG CCAGTCTCCA 
AAGAAGCCGT TCAATAGCAT TATTGAGCAC CTGTTGGTGG TGTTCCCGTG TTACAGCAGC 
ACGGAGCTTG CTGGTTTTAT TAAAAAAGTT AGAAACAAAA ACAAGACCTC TCTGTCTGGA 
TTGAGTATTG ACGAAATTGT GCAAAGAGTG ACAGAACACG TCCTTGATGA AGAAAGAAAG 
AAAAAGCCAA GTCCAGGAAA GGGCTGGAGG CTGTCTGCAC CCAGCTCTGC TGTTGCTGTG 
CCCAGGGCTG CCCAGGACCC GCCCTCCATG CCTGTCGGAG CATCGCCCGA AACCAGGGGG 
CCGAGGACCG AGGACGTCCC TGTGTCCTGC GCAAATTCCT GTGAAATGTG CCGAGAAGTA 
TTCAAGTCCA AACACGTGCG TGAGCTGCAG TGTGGGCACA GGCTTCACAA GGGGTGCTTC 
AAGCAGTGGC TGAAGAGCCA GCGCACCTGC CCAGCCTGCC TGGGTCACGA CCTCCTGTCA 
GAGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_010801678.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010799980.1 Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X7, mRNA.

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CloneID	OBa46605
Clone ID Related Accession (Same CDS sequence)	XM_010801678.2
Accession Version	XM_010801678.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5214bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TTC3 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103826.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010801678.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGATT TTCCCCAGGG AAATTTTGCA GATTATGCCT TGTTAGAAGA TTGCCCTTAT 
GTGGATGATT GTATCTTTGC CCCTGAGTTT ATGTCCAGTG ATTATGTTCG TGTGACTCAG 
CTTTACTGTG ATGGAGTGGG TAGACAGTAT AAAGATTATG CCCAAACTGA GGGAAACTTA 
GAATTTGACA TCTGCACTCT GTGGTGTAGT CAACCTATTT CTGTCCTGCA AGATTACTGT 
GATGCCATTA AAGTCCACAT CTTCTGGCCA CTTCTGTTTC AACGTCAGCA GAGCTCTATA 
ATAACACGGA TGCACTCCTG TGTAAGGACC AGCGGTTCTC ATGCTTGTGA GATAAGTTTG 
AAGAAATTAC AGCAGCTTGA ACTGATGGAA GATATTGTGG ATTTGGCAAA GAAAGTTGTG 
AATGATACAT TCTTTATCGG AGGCTTATTG AGAATTGGCT ATAAAATAGA GAATAAAATC 
TTGGCAATGG AAGAAGCTTT GAATTGGGTG AAATACACAG GCGATGTAAC AATTTTATCT 
AAATTAGGAG CAGTTGATAA TTGTTGGCGT ATGTTAAGTA TTTTCTTTAC TGAATACAAG 
TATCACATAA CTAAAGTTGT AACAGAAAAC TGCAGTTTGC TTGAAGAATT TAAAACTCAA 
AGTTGTGCGG AGTGTCTAGA GCAAGGAGAA CTAATGAAAA TGAAAGGAAA TGAAGAGTTT 
TCCAAAGAAA GATATGATAT AGCTATTATC TATTACACCA GAGCCATTGA ATGTAGGCCT 
GAAAATCACC TGCTTTATGG TAACCGAGCT CTTTGTTTTC TTCGTACTGG ACAATTCAGA 
AATGCACTTG GTGATGGAAA GAGAGCTACC ATTCTGAAGA ACAATTGGAC AAAGGGTCAT 
TATCGTTATT GTGCTGCTCT TTCTATGCTG GGGGAATATG ACTGGGCCCT GCAAGCAAAC 
ATAAAAGCTC AAAAACTCTG TAAAAATGAC CCTGAGGGAA TCAAGGATCT AATTCAGCAG 
CATGTAAAGT TACAAAAACA AATAGAAGAC CTGCAAGGTC AAACACCAGC TAGTAATCCA 
GTCAAAGCCT TCTGTGAAAG CAGGGCCTAC ATACCTAGTT TATCAGCACC TGCATTTAGT 
ACTTCACTTA ACTTTGTGGA AACTGAAAAA AATTTCAGAA AAACTAACCA CGAAATGGCA 
AACGGTGGTA ATCAGAATCC AAAGGTGGTA GATGAAGCTC TGAAAGTAGA TGATTGTGAC 
TGTCATCCTG AATTTGTACC GCCATCAAGT CAGCCTCCAA AATATAAAGG GAAACAAAAA 
TCTCGAAACA ATGAATTGGA GAAGTTCAGC CCTGGTTCAC AAGAAACTTT ACCAGTAGAT 
TTAAAAAACA TCTTGGAGAA ACAGTTTTCT AAATCTTCCA GAGCCGCACA CCAGGATTTT 
GCTAATATAA TGAAAATGCT GAGAAGTTTA ATTCAGGATG GCTACACGGC CTTGCTGGAG 
CAGCGCTGCC GCAGCGCCGC CCAAGCCTTC ACAGAGTTGC TCAACGGTTT AGATCCTCAA 
AAAATAAAGC AATTGAACCT GGCCATGATT AACTATGTGT TAGTAGTCTA CGGACTTGCC 
ATTTCACTCC TTGGAATAGG ACAGCCCGAG GAGTTATCTG AAGCTGAAAA CCAGTTCAAG 
AGGATGATTG AACACTACCC CAATGAGGGG CTTGATTGCT TGGCCTACTG TGGAATTGGA 
AAAGTGTATT TGAAAAAAAA CAGATTTTTA GAAGCTCTTA ATCATTTTGA GAAAGCAAGA 
ACCTTGATCT GCCGTCTTCC TGGAGTGTTA ACTTGGCCCA CAAGTAATGT GGTTATTGAG 
GAGACTCAGC CAGGGAAGAT AAAGATGCTG TTAGAGAAAT TTGTTGAAGA ATGCAGGTTC 
CCTCCAGTGC CGGATGCCAC TTGTTGCTAT CAGAAGTGCC ATGGATATTC CAAAATCCAG 
ATATATATAA CTGATCCAGA CTTTAAGGGT TTTATACGGA TCAGCTGTTG CCAGTATTGT 
AAAATAGAAT TTCACATGAA CTGCTGGAAG AAGTTAAAAA CAACAACCTT CAATGATAAA 
ATTGACAAGG ATTTTCTGCA AGGAATCTGT CTTACTCCTG ATTGTGAAGG AATCATTTCA 
AAGATTATCA TCTTCAGCAG TGGTGGTCAA GTTAAATGTG AATTTGAACA CAAGGTCATT 
AAAGAAAAGG TTCCTCCAAG ACCTATTCTG AAACAGAAAT GTTCTAGCCT AGAGAAGTTG 
AGGCTGAAAG AAGACAAAAA ATTGAAGCGA AAGCTCCAAA AAAAGGAAGC AAAAAAATTA 
GCACAAGAAA GACTGGAAGA GGACTTAAGG GAAAGTAACC CACCAAAAAC TGAAGAGCAG 
AGAGAAACTA TCAACCATGT CCAAAGTTGC CAGTTCCTTG ATGACAGAAT TCTGCAGTGC 
ATAAAGCAGT ACGCTGACAA GATTAAGTCC GGGATACTGA ATACTTCCAA GCTTCTCAAA 
GAATTGCTTT CTTGGAAGGT TTTAACCACA GAAGACTACA CAACGTGTTT TTCTAGCAGA 
AATTTCCTGA ATGAAGCAGT GGACTATGTC ATTCGTCATT TGATCCAAGA AAAGAATAGA 
GTAAAGACAA GGGTATTTCT GCATGTTTTG AGTGAGCTTA AAGAAGTGGA CCCAAAACTA 
GCCACCTGGA TCCAGAAACT GAATAGCTTT GGCTTAGATG CCACAGGACC TTTCTTTTCT 
CGTTATGGGG CTCCTCTTAA GGAGCTTGAT TTTAGCATCA TGACTTTTCT CTGGAATGAG 
AAATACGGTC ATAAGCTAGG CTCTATAGAA GGAAAGCAAC TTGATTATTT CTATGAGCCT 
ACATCACTGA AGGAAGCGCG GTGTTTAATA TGGCTGCTAG AAGAACACAG AGACAAGTTC 
CCAGCACTGC ATAATGCCTT GGACGAGTTC TTTGATATAA TGGACAGCCG CTGCACTGTA 
TTAAGGAAAC AGGACAGTGG TGACGTGCCA TTTAATTCTA CCAAGATAAA AAACAAAGGC 
AAGAAAAAGA AGCCAAAAGA TTCAAAGCCT ATGTTAGTTG GATCTGGAAC ACCTTCAGTC 
ACACCAAACA ATGAGACTGT CACTTCAGGT GAAGACCATA TCAAACGCAG TTTAAGTTCT 
GCGGGCCCCT TTGTAGTGCC TGATCACCTT CGGCAGGATA TAGAAGAATT TGAAGCTCTG 
TATGAACAGC ATAGTAATGA GTATGTTGTC CGTAATAAGA AGCTGTGGGA CATAAACCCA 
AAACAGAAAT GTTCAACTCT ATACGATTAT TTCTCTCAGT TGTTGGAGGA ACATGGTCCC 
TTGGACATGA GTAACAAAAT GTTTTCTGAA GAATATGAGT TCTTCCCAGA AGAAACTCAA 
CAGATACTAG AAAAAGCAGG AGGCTTGAAA GCGTTTCTCC TGGGATGTCC TCGTTTTGTT 
GTGATTGATA ACTGTATCGC ATTGAAGAAA GTTGCATTAC GGCTCAAGAA AAAGAGAAAG 
AAGAAAAACA TTAAAACAAA AGTAGAAGAA ATTTCAAAAA CAGGAGAGTA TTTACGAGTT 
AAACTACCAC TGAATCCAAC TGCTAGGGAA TTTAAACCAG ATGTAAAGTC CAAACCAGTG 
TCTGACTTAC CTTCAGCACC AGCTTCTGAG GACATGAAGC CTCAGCCTGC ACCTGCTAAT 
TCTCCCCAAT CAGCTCGTGA CACTGGGAAG CCAAAACCAG TTTTCGATAA TTCTTCCAGA 
TCAGTTTCTG AGGATGAGAA GCCCAAAGGG ATGTCTTCTG ATTCTCCCAG GCCAGTCTTT 
GAGGATACCG GTCACAAGCC GGTCTCCTCT AATTCTCCCA AACCAGTTCC TGAGGAAGTG 
AAAGCGACCT ATTGGCCTCG ACCCCAGTTG GTCACAGGAT ACTGTACATA TCTTCCCTTT 
CGGGGATTTG ACATTACCCA GACTCCACCA GCCTTCATCA GTGTGTTACC GACTTTGCCC 
CAGTATAGCA TTTACACCCC TCTGGCCAGT GTTCCTACTG AGTATCACCT GCAAAGACCA 
ATACCAGTGG TGCCGTCTTT TGTAGCCAGT GACATAACGG AAGTGCATTT TGAGGCTCAT 
CATTTCAGTG CTGAGAATGC CCCTGGTACC CAGATTGCCT CCGAAACACA GATCCTTGAG 
GAATCTTTGG AAACACCAGT GAAGTCAGAG TGCAGCTATG ATGGTGCTGA TATAGTCCAG 
AGTGAGGCTA GCAGAAACGA TGACTACTGT GGCGATTCTG CCAAATGGGA AGTAAATCCA 
GAAAGCACCA ATGAAGTAAC AAATATTCCA TGTACACAAG TGGTTGCTGT ACAGGTGTCT 
TGGAATATAA CACATCAGGA AGTCAATACT GAGCCATATG GTCCTTTTGA GGCACAACAG 
GGGGGTATTT CACAGGTTGA AAAGGAGTAC CAGGTTTTAC AAGAGCAGCT TAAGGAAGCA 
TGTGAAAATT ATGAACACAG CAATGTCAAG GGCTCAGAAG AAACCAAGGA CTTGGAAGAA 
AAGTTGAAAA GGAATGTTGA AGAAAATAAG ATCTCAAAGA CAGAACTAGA TTGGTTCCTT 
GAAGATTTGA ACAAGGAAAT TAAAAAATGG GAACAGGAGA AAAAAGAAAT CCAAGAAAGA 
CTAAAGGCAC TGAAGAAGAA AATGAAAAAG GTGTTAAATG CCAGCAAAAT GTATACGCAG 
GAAAATGATG GAAAGGAAAA GGCACTTGAA TTACTTCTGG ATCAGTCCCT TGAAATCAGT 
AATACATTTA CAAATGAGAA AATGAAAATA GAAGAGAGTA TAAAGAAAGG GAAAGAGCAT 
TATGAAGAGA GTCTTCAAAG GGCTGTGGAT GCAGAGGTAT CTGTGCTTGA AAACTGGAAG 
GACATGGAAG TGTGTAAGTT ACAGAGCATG GAGTCACAAG CCGAAAGGTT TCTCAAGAAT 
CTGAAGCTAA TGAGCAGTGA TTCGGCAGCG TATTCTGACA TGGACTCAGA CATACATTCA 
TGGGAATCGT TTCTTTCTGA TGTTAGAGAA GAAATTGAGA AAGCAAAGTC TCAGTTTGAA 
GAACAAATTC AGGCAATTAA ATATGGTTCT CAGCTCAGTG AACTTCCTAA AGTGCAGATT 
TCTGAGCTTT CATTTCCTAC CTGTAACACG CACGGAGCTT GCTGGTTTTA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010799980.1
CDS	12..5225
Translation	MADFPQGNFADYALLEDCPYVDDCIFAPEFMSSDYVRVTQLYCDGVGRQYKDYAQTEGNLEFDICTLWCSQPISVLQDYCDAIKVHIFWPLLFQRQQSSIITRMHSCVRTSGSHACEISLKKLQQLELMEDIVDLAKKVVNDTFFIGGLLRIGYKIENKILAMEEALNWVKYTGDVTILSKLGAVDNCWRMLSIFFTEYKYHITKVVTENCSLLEEFKTQSCAECLEQGELMKMKGNEEFSKERYDIAIIYYTRAIECRPENHLLYGNRALCFLRTGQFRNALGDGKRATILKNNWTKGHYRYCAALSMLGEYDWALQANIKAQKLCKNDPEGIKDLIQQHVKLQKQIEDLQGQTPASNPVKAFCESRAYIPSLSAPAFSTSLNFVETEKNFRKTNHEMANGGNQNPKVVDEALKVDDCDCHPEFVPPSSQPPKYKGKQKSRNNELEKFSPGSQETLPVDLKNILEKQFSKSSRAAHQDFANIMKMLRSLIQDGYTALLEQRCRSAAQAFTELLNGLDPQKIKQLNLAMINYVLVVYGLAISLLGIGQPEELSEAENQFKRMIEHYPNEGLDCLAYCGIGKVYLKKNRFLEALNHFEKARTLICRLPGVLTWPTSNVVIEETQPGKIKMLLEKFVEECRFPPVPDATCCYQKCHGYSKIQIYITDPDFKGFIRISCCQYCKIEFHMNCWKKLKTTTFNDKIDKDFLQGICLTPDCEGIISKIIIFSSGGQVKCEFEHKVIKEKVPPRPILKQKCSSLEKLRLKEDKKLKRKLQKKEAKKLAQERLEEDLRESNPPKTEEQRETINHVQSCQFLDDRILQCIKQYADKIKSGILNTSKLLKELLSWKVLTTEDYTTCFSSRNFLNEAVDYVIRHLIQEKNRVKTRVFLHVLSELKEVDPKLATWIQKLNSFGLDATGPFFSRYGAPLKELDFSIMTFLWNEKYGHKLGSIEGKQLDYFYEPTSLKEARCLIWLLEEHRDKFPALHNALDEFFDIMDSRCTVLRKQDSGDVPFNSTKIKNKGKKKKPKDSKPMLVGSGTPSVTPNNETVTSGEDHIKRSLSSAGPFVVPDHLRQDIEEFEALYEQHSNEYVVRNKKLWDINPKQKCSTLYDYFSQLLEEHGPLDMSNKMFSEEYEFFPEETQQILEKAGGLKAFLLGCPRFVVIDNCIALKKVALRLKKKRKKKNIKTKVEEISKTGEYLRVKLPLNPTAREFKPDVKSKPVSDLPSAPASEDMKPQPAPANSPQSARDTGKPKPVFDNSSRSVSEDEKPKGMSSDSPRPVFEDTGHKPVSSNSPKPVPEEVKATYWPRPQLVTGYCTYLPFRGFDITQTPPAFISVLPTLPQYSIYTPLASVPTEYHLQRPIPVVPSFVASDITEVHFEAHHFSAENAPGTQIASETQILEESLETPVKSECSYDGADIVQSEASRNDDYCGDSAKWEVNPESTNEVTNIPCTQVVAVQVSWNITHQEVNTEPYGPFEAQQGGISQVEKEYQVLQEQLKEACENYEHSNVKGSEETKDLEEKLKRNVEENKISKTELDWFLEDLNKEIKKWEQEKKEIQERLKALKKKMKKVLNASKMYTQENDGKEKALELLLDQSLEISNTFTNEKMKIEESIKKGKEHYEESLQRAVDAEVSVLENWKDMEVCKLQSMESQAERFLKNLKLMSSDSAAYSDMDSDIHSWESFLSDVREEIEKAKSQFEEQIQAIKYGSQLSELPKVQISELSFPTCNTHGACWFY

Target ORF information:

RefSeq Version	XM_010801678.2
Organism	Bos taurus(cattle)
Definition	Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010801678.2

ORF Insert Sequence:

ATGGCCGATT TTCCCCAGGG AAATTTTGCA GATTATGCCT TGTTAGAAGA TTGCCCTTAT 
GTGGATGATT GTATCTTTGC CCCTGAGTTT ATGTCCAGTG ATTATGTTCG TGTGACTCAG 
CTTTACTGTG ATGGAGTGGG TAGACAGTAT AAAGATTATG CCCAAACTGA GGGAAACTTA 
GAATTTGACA TCTGCACTCT GTGGTGTAGT CAACCTATTT CTGTCCTGCA AGATTACTGT 
GATGCCATTA AAGTCCACAT CTTCTGGCCA CTTCTGTTTC AACGTCAGCA GAGCTCTATA 
ATAACACGGA TGCACTCCTG TGTAAGGACC AGCGGTTCTC ATGCTTGTGA GATAAGTTTG 
AAGAAATTAC AGCAGCTTGA ACTGATGGAA GATATTGTGG ATTTGGCAAA GAAAGTTGTG 
AATGATACAT TCTTTATCGG AGGCTTATTG AGAATTGGCT ATAAAATAGA GAATAAAATC 
TTGGCAATGG AAGAAGCTTT GAATTGGGTG AAATACACAG GCGATGTAAC AATTTTATCT 
AAATTAGGAG CAGTTGATAA TTGTTGGCGT ATGTTAAGTA TTTTCTTTAC TGAATACAAG 
TATCACATAA CTAAAGTTGT AACAGAAAAC TGCAGTTTGC TTGAAGAATT TAAAACTCAA 
AGTTGTGCGG AGTGTCTAGA GCAAGGAGAA CTAATGAAAA TGAAAGGAAA TGAAGAGTTT 
TCCAAAGAAA GATATGATAT AGCTATTATC TATTACACCA GAGCCATTGA ATGTAGGCCT 
GAAAATCACC TGCTTTATGG TAACCGAGCT CTTTGTTTTC TTCGTACTGG ACAATTCAGA 
AATGCACTTG GTGATGGAAA GAGAGCTACC ATTCTGAAGA ACAATTGGAC AAAGGGTCAT 
TATCGTTATT GTGCTGCTCT TTCTATGCTG GGGGAATATG ACTGGGCCCT GCAAGCAAAC 
ATAAAAGCTC AAAAACTCTG TAAAAATGAC CCTGAGGGAA TCAAGGATCT AATTCAGCAG 
CATGTAAAGT TACAAAAACA AATAGAAGAC CTGCAAGGTC AAACACCAGC TAGTAATCCA 
GTCAAAGCCT TCTGTGAAAG CAGGGCCTAC ATACCTAGTT TATCAGCACC TGCATTTAGT 
ACTTCACTTA ACTTTGTGGA AACTGAAAAA AATTTCAGAA AAACTAACCA CGAAATGGCA 
AACGGTGGTA ATCAGAATCC AAAGGTGGTA GATGAAGCTC TGAAAGTAGA TGATTGTGAC 
TGTCATCCTG AATTTGTACC GCCATCAAGT CAGCCTCCAA AATATAAAGG GAAACAAAAA 
TCTCGAAACA ATGAATTGGA GAAGTTCAGC CCTGGTTCAC AAGAAACTTT ACCAGTAGAT 
TTAAAAAACA TCTTGGAGAA ACAGTTTTCT AAATCTTCCA GAGCCGCACA CCAGGATTTT 
GCTAATATAA TGAAAATGCT GAGAAGTTTA ATTCAGGATG GCTACACGGC CTTGCTGGAG 
CAGCGCTGCC GCAGCGCCGC CCAAGCCTTC ACAGAGTTGC TCAACGGTTT AGATCCTCAA 
AAAATAAAGC AATTGAACCT GGCCATGATT AACTATGTGT TAGTAGTCTA CGGACTTGCC 
ATTTCACTCC TTGGAATAGG ACAGCCCGAG GAGTTATCTG AAGCTGAAAA CCAGTTCAAG 
AGGATGATTG AACACTACCC CAATGAGGGG CTTGATTGCT TGGCCTACTG TGGAATTGGA 
AAAGTGTATT TGAAAAAAAA CAGATTTTTA GAAGCTCTTA ATCATTTTGA GAAAGCAAGA 
ACCTTGATCT GCCGTCTTCC TGGAGTGTTA ACTTGGCCCA CAAGTAATGT GGTTATTGAG 
GAGACTCAGC CAGGGAAGAT AAAGATGCTG TTAGAGAAAT TTGTTGAAGA ATGCAGGTTC 
CCTCCAGTGC CGGATGCCAC TTGTTGCTAT CAGAAGTGCC ATGGATATTC CAAAATCCAG 
ATATATATAA CTGATCCAGA CTTTAAGGGT TTTATACGGA TCAGCTGTTG CCAGTATTGT 
AAAATAGAAT TTCACATGAA CTGCTGGAAG AAGTTAAAAA CAACAACCTT CAATGATAAA 
ATTGACAAGG ATTTTCTGCA AGGAATCTGT CTTACTCCTG ATTGTGAAGG AATCATTTCA 
AAGATTATCA TCTTCAGCAG TGGTGGTCAA GTTAAATGTG AATTTGAACA CAAGGTCATT 
AAAGAAAAGG TTCCTCCAAG ACCTATTCTG AAACAGAAAT GTTCTAGCCT AGAGAAGTTG 
AGGCTGAAAG AAGACAAAAA ATTGAAGCGA AAGCTCCAAA AAAAGGAAGC AAAAAAATTA 
GCACAAGAAA GACTGGAAGA GGACTTAAGG GAAAGTAACC CACCAAAAAC TGAAGAGCAG 
AGAGAAACTA TCAACCATGT CCAAAGTTGC CAGTTCCTTG ATGACAGAAT TCTGCAGTGC 
ATAAAGCAGT ACGCTGACAA GATTAAGTCC GGGATACTGA ATACTTCCAA GCTTCTCAAA 
GAATTGCTTT CTTGGAAGGT TTTAACCACA GAAGACTACA CAACGTGTTT TTCTAGCAGA 
AATTTCCTGA ATGAAGCAGT GGACTATGTC ATTCGTCATT TGATCCAAGA AAAGAATAGA 
GTAAAGACAA GGGTATTTCT GCATGTTTTG AGTGAGCTTA AAGAAGTGGA CCCAAAACTA 
GCCACCTGGA TCCAGAAACT GAATAGCTTT GGCTTAGATG CCACAGGACC TTTCTTTTCT 
CGTTATGGGG CTCCTCTTAA GGAGCTTGAT TTTAGCATCA TGACTTTTCT CTGGAATGAG 
AAATACGGTC ATAAGCTAGG CTCTATAGAA GGAAAGCAAC TTGATTATTT CTATGAGCCT 
ACATCACTGA AGGAAGCGCG GTGTTTAATA TGGCTGCTAG AAGAACACAG AGACAAGTTC 
CCAGCACTGC ATAATGCCTT GGACGAGTTC TTTGATATAA TGGACAGCCG CTGCACTGTA 
TTAAGGAAAC AGGACAGTGG TGACGTGCCA TTTAATTCTA CCAAGATAAA AAACAAAGGC 
AAGAAAAAGA AGCCAAAAGA TTCAAAGCCT ATGTTAGTTG GATCTGGAAC ACCTTCAGTC 
ACACCAAACA ATGAGACTGT CACTTCAGGT GAAGACCATA TCAAACGCAG TTTAAGTTCT 
GCGGGCCCCT TTGTAGTGCC TGATCACCTT CGGCAGGATA TAGAAGAATT TGAAGCTCTG 
TATGAACAGC ATAGTAATGA GTATGTTGTC CGTAATAAGA AGCTGTGGGA CATAAACCCA 
AAACAGAAAT GTTCAACTCT ATACGATTAT TTCTCTCAGT TGTTGGAGGA ACATGGTCCC 
TTGGACATGA GTAACAAAAT GTTTTCTGAA GAATATGAGT TCTTCCCAGA AGAAACTCAA 
CAGATACTAG AAAAAGCAGG AGGCTTGAAA GCGTTTCTCC TGGGATGTCC TCGTTTTGTT 
GTGATTGATA ACTGTATCGC ATTGAAGAAA GTTGCATTAC GGCTCAAGAA AAAGAGAAAG 
AAGAAAAACA TTAAAACAAA AGTAGAAGAA ATTTCAAAAA CAGGAGAGTA TTTACGAGTT 
AAACTACCAC TGAATCCAAC TGCTAGGGAA TTTAAACCAG ATGTAAAGTC CAAACCAGTG 
TCTGACTTAC CTTCAGCACC AGCTTCTGAG GACATGAAGC CTCAGCCTGC ACCTGCTAAT 
TCTCCCCAAT CAGCTCGTGA CACTGGGAAG CCAAAACCAG TTTTCGATAA TTCTTCCAGA 
TCAGTTTCTG AGGATGAGAA GCCCAAAGGG ATGTCTTCTG ATTCTCCCAG GCCAGTCTTT 
GAGGATACCG GTCACAAGCC GGTCTCCTCT AATTCTCCCA AACCAGTTCC TGAGGAAGTG 
AAAGCGACCT ATTGGCCTCG ACCCCAGTTG GTCACAGGAT ACTGTACATA TCTTCCCTTT 
CGGGGATTTG ACATTACCCA GACTCCACCA GCCTTCATCA GTGTGTTACC GACTTTGCCC 
CAGTATAGCA TTTACACCCC TCTGGCCAGT GTTCCTACTG AGTATCACCT GCAAAGACCA 
ATACCAGTGG TGCCGTCTTT TGTAGCCAGT GACATAACGG AAGTGCATTT TGAGGCTCAT 
CATTTCAGTG CTGAGAATGC CCCTGGTACC CAGATTGCCT CCGAAACACA GATCCTTGAG 
GAATCTTTGG AAACACCAGT GAAGTCAGAG TGCAGCTATG ATGGTGCTGA TATAGTCCAG 
AGTGAGGCTA GCAGAAACGA TGACTACTGT GGCGATTCTG CCAAATGGGA AGTAAATCCA 
GAAAGCACCA ATGAAGTAAC AAATATTCCA TGTACACAAG TGGTTGCTGT ACAGGTGTCT 
TGGAATATAA CACATCAGGA AGTCAATACT GAGCCATATG GTCCTTTTGA GGCACAACAG 
GGGGGTATTT CACAGGTTGA AAAGGAGTAC CAGGTTTTAC AAGAGCAGCT TAAGGAAGCA 
TGTGAAAATT ATGAACACAG CAATGTCAAG GGCTCAGAAG AAACCAAGGA CTTGGAAGAA 
AAGTTGAAAA GGAATGTTGA AGAAAATAAG ATCTCAAAGA CAGAACTAGA TTGGTTCCTT 
GAAGATTTGA ACAAGGAAAT TAAAAAATGG GAACAGGAGA AAAAAGAAAT CCAAGAAAGA 
CTAAAGGCAC TGAAGAAGAA AATGAAAAAG GTGTTAAATG CCAGCAAAAT GTATACGCAG 
GAAAATGATG GAAAGGAAAA GGCACTTGAA TTACTTCTGG ATCAGTCCCT TGAAATCAGT 
AATACATTTA CAAATGAGAA AATGAAAATA GAAGAGAGTA TAAAGAAAGG GAAAGAGCAT 
TATGAAGAGA GTCTTCAAAG GGCTGTGGAT GCAGAGGTAT CTGTGCTTGA AAACTGGAAG 
GACATGGAAG TGTGTAAGTT ACAGAGCATG GAGTCACAAG CCGAAAGGTT TCTCAAGAAT 
CTGAAGCTAA TGAGCAGTGA TTCGGCAGCG TATTCTGACA TGGACTCAGA CATACATTCA 
TGGGAATCGT TTCTTTCTGA TGTTAGAGAA GAAATTGAGA AAGCAAAGTC TCAGTTTGAA 
GAACAAATTC AGGCAATTAA ATATGGTTCT CAGCTCAGTG AACTTCCTAA AGTGCAGATT 
TCTGAGCTTT CATTTCCTAC CTGTAACACG CACGGAGCTT GCTGGTTTTA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_010801679.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010799981.1 Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X8, mRNA.

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CloneID	OBa46606
Clone ID Related Accession (Same CDS sequence)	XM_010801679.2
Accession Version	XM_010801679.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5265bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TTC3 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103826.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010801679.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATGA AAGGAAATGA AGAGTTTTCC AAAGAAAGAT ATGATATAGC TATTATCTAT 
TACACCAGAG CCATTGAATG TAGGCCTGAA AATCACCTGC TTTATGGTAA CCGAGCTCTT 
TGTTTTCTTC GTACTGGACA ATTCAGAAAT GCACTTGGTG ATGGAAAGAG AGCTACCATT 
CTGAAGAACA ATTGGACAAA GGGTCATTAT CGTTATTGTG CTGCTCTTTC TATGCTGGGG 
GAATATGACT GGGCCCTGCA AGCAAACATA AAAGCTCAAA AACTCTGTAA AAATGACCCT 
GAGGGAATCA AGGATCTAAT TCAGCAGCAT GTAAAGTTAC AAAAACAAAT AGAAGACCTG 
CAAGGTCAAA CACCAGCTAG TAATCCAGTC AAAGCCTTCT GTGAAAGCAG GGCCTACATA 
CCTAGTTTAT CAGCACCTGC ATTTAGTACT TCACTTAACT TTGTGGAAAC TGAAAAAAAT 
TTCAGAAAAA CTAACCACGA AATGGCAAAC GGTGGTAATC AGAATCCAAA GGTGGTAGAT 
GAAGCTCTGA AAGTAGATGA TTGTGACTGT CATCCTGAAT TTGTACCGCC ATCAAGTCAG 
CCTCCAAAAT ATAAAGGGAA ACAAAAATCT CGAAACAATG AATTGGAGAA GTTCAGCCCT 
GGTTCACAAG AAACTTTACC AGTAGATTTA AAAAACATCT TGGAGAAACA GTTTTCTAAA 
TCTTCCAGAG CCGCACACCA GGATTTTGCT AATATAATGA AAATGCTGAG AAGTTTAATT 
CAGGATGGCT ACACGGCCTT GCTGGAGCAG CGCTGCCGCA GCGCCGCCCA AGCCTTCACA 
GAGTTGCTCA ACGGTTTAGA TCCTCAAAAA ATAAAGCAAT TGAACCTGGC CATGATTAAC 
TATGTGTTAG TAGTCTACGG ACTTGCCATT TCACTCCTTG GAATAGGACA GCCCGAGGAG 
TTATCTGAAG CTGAAAACCA GTTCAAGAGG ATGATTGAAC ACTACCCCAA TGAGGGGCTT 
GATTGCTTGG CCTACTGTGG AATTGGAAAA GTGTATTTGA AAAAAAACAG ATTTTTAGAA 
GCTCTTAATC ATTTTGAGAA AGCAAGAACC TTGATCTGCC GTCTTCCTGG AGTGTTAACT 
TGGCCCACAA GTAATGTGGT TATTGAGGAG ACTCAGCCAG GGAAGATAAA GATGCTGTTA 
GAGAAATTTG TTGAAGAATG CAGGTTCCCT CCAGTGCCGG ATGCCACTTG TTGCTATCAG 
AAGTGCCATG GATATTCCAA AATCCAGATA TATATAACTG ATCCAGACTT TAAGGGTTTT 
ATACGGATCA GCTGTTGCCA GTATTGTAAA ATAGAATTTC ACATGAACTG CTGGAAGAAG 
TTAAAAACAA CAACCTTCAA TGATAAAATT GACAAGGATT TTCTGCAAGG AATCTGTCTT 
ACTCCTGATT GTGAAGGAAT CATTTCAAAG ATTATCATCT TCAGCAGTGG TGGTCAAGTT 
AAATGTGAAT TTGAACACAA GGTCATTAAA GAAAAGGTTC CTCCAAGACC TATTCTGAAA 
CAGAAATGTT CTAGCCTAGA GAAGTTGAGG CTGAAAGAAG ACAAAAAATT GAAGCGAAAG 
CTCCAAAAAA AGGAAGCAAA AAAATTAGCA CAAGAAAGAC TGGAAGAGGA CTTAAGGGAA 
AGTAACCCAC CAAAAACTGA AGAGCAGAGA GAAACTATCA ACCATGTCCA AAGTTGCCAG 
TTCCTTGATG ACAGAATTCT GCAGTGCATA AAGCAGTACG CTGACAAGAT TAAGTCCGGG 
ATACTGAATA CTTCCAAGCT TCTCAAAGAA TTGCTTTCTT GGAAGGTTTT AACCACAGAA 
GACTACACAA CGTGTTTTTC TAGCAGAAAT TTCCTGAATG AAGCAGTGGA CTATGTCATT 
CGTCATTTGA TCCAAGAAAA GAATAGAGTA AAGACAAGGG TATTTCTGCA TGTTTTGAGT 
GAGCTTAAAG AAGTGGACCC AAAACTAGCC ACCTGGATCC AGAAACTGAA TAGCTTTGGC 
TTAGATGCCA CAGGACCTTT CTTTTCTCGT TATGGGGCTC CTCTTAAGGA GCTTGATTTT 
AGCATCATGA CTTTTCTCTG GAATGAGAAA TACGGTCATA AGCTAGGCTC TATAGAAGGA 
AAGCAACTTG ATTATTTCTA TGAGCCTACA TCACTGAAGG AAGCGCGGTG TTTAATATGG 
CTGCTAGAAG AACACAGAGA CAAGTTCCCA GCACTGCATA ATGCCTTGGA CGAGTTCTTT 
GATATAATGG ACAGCCGCTG CACTGTATTA AGGAAACAGG ACAGTGGTGA CGTGCCATTT 
AATTCTACCA AGATAAAAAA CAAAGGCAAG AAAAAGAAGC CAAAAGATTC AAAGCCTATG 
TTAGTTGGAT CTGGAACACC TTCAGTCACA CCAAACAATG AGACTGTCAC TTCAGGTGAA 
GACCATATCA AACGCAGTTT AAGTTCTGCG GGCCCCTTTG TAGTGCCTGA TCACCTTCGG 
CAGGATATAG AAGAATTTGA AGCTCTGTAT GAACAGCATA GTAATGAGTA TGTTGTCCGT 
AATAAGAAGC TGTGGGACAT AAACCCAAAA CAGAAATGTT CAACTCTATA CGATTATTTC 
TCTCAGTTGT TGGAGGAACA TGGTCCCTTG GACATGAGTA ACAAAATGTT TTCTGAAGAA 
TATGAGTTCT TCCCAGAAGA AACTCAACAG ATACTAGAAA AAGCAGGAGG CTTGAAAGCG 
TTTCTCCTGG GATGTCCTCG TTTTGTTGTG ATTGATAACT GTATCGCATT GAAGAAAGTT 
GCATTACGGC TCAAGAAAAA GAGAAAGAAG AAAAACATTA AAACAAAAGT AGAAGAAATT 
TCAAAAACAG GAGAGTATTT ACGAGTTAAA CTACCACTGA ATCCAACTGC TAGGGAATTT 
AAACCAGATG TAAAGTCCAA ACCAGTGTCT GACTTACCTT CAGCACCAGC TTCTGAGGAC 
ATGAAGCCTC AGCCTGCACC TGCTAATTCT CCCCAATCAG CTCGTGACAC TGGGAAGCCA 
AAACCAGTTT TCGATAATTC TTCCAGATCA GTTTCTGAGG ATGAGAAGCC CAAAGGGATG 
TCTTCTGATT CTCCCAGGCC AGTCTTTGAG GATACCGGTC ACAAGCCGGT CTCCTCTAAT 
TCTCCCAAAC CAGTTCCTGA GGAAGTGAAA GCGACCTATT GGCCTCGACC CCAGTTGGTC 
ACAGGATACT GTACATATCT TCCCTTTCGG GGATTTGACA TTACCCAGAC TCCACCAGCC 
TTCATCAGTG TGTTACCGAC TTTGCCCCAG TATAGCATTT ACACCCCTCT GGCCAGTGTT 
CCTACTGAGT ATCACCTGCA AAGACCAATA CCAGTGGTGC CGTCTTTTGT AGCCAGTGAC 
ATAACGGAAG TGCATTTTGA GGCTCATCAT TTCAGTGCTG AGAATGCCCC TGGTACCCAG 
ATTGCCTCCG AAACACAGAT CCTTGAGGAA TCTTTGGAAA CACCAGTGAA GTCAGAGTGC 
AGCTATGATG GTGCTGATAT AGTCCAGAGT GAGGCTAGCA GAAACGATGA CTACTGTGGC 
GATTCTGCCA AATGGGAAGT AAATCCAGAA AGCACCAATG AAGTAACAAA TATTCCATGT 
ACACAAGTGG TTGCTGTACA GGTGTCTTGG AATATAACAC ATCAGGAAGT CAATACTGAG 
CCATATGGTC CTTTTGAGGC ACAACAGGGG GGTATTTCAC AGGTTGAAAA GGAGTACCAG 
GTTTTACAAG AGCAGCTTAA GGAAGCATGT GAAAATTATG AACACAGCAA TGTCAAGGGC 
TCAGAAGAAA CCAAGGACTT GGAAGAAAAG TTGAAAAGGA ATGTTGAAGA AAATAAGATC 
TCAAAGACAG AACTAGATTG GTTCCTTGAA GATTTGAACA AGGAAATTAA AAAATGGGAA 
CAGGAGAAAA AAGAAATCCA AGAAAGACTA AAGGCACTGA AGAAGAAAAT GAAAAAGGTG 
TTAAATGCCA GCAAAATGTA TACGCAGGAA AATGATGGAA AGGAAAAGGC ACTTGAATTA 
CTTCTGGATC AGTCCCTTGA AATCAGTAAT ACATTTACAA ATGAGAAAAT GAAAATAGAA 
GAGAGTATAA AGAAAGGGAA AGAGCATTAT GAAGAGAGTC TTCAAAGGGC TGTGGATGCA 
GAGGTATCTG TGCTTGAAAA CTGGAAGGAC ATGGAAGTGT GTAAGTTACA GAGCATGGAG 
TCACAAGCCG AAAGGTTTCT CAAGAATCTG AAGCTAATGA GCAGTGATTC GGCAGCGTAT 
TCTGACATGG ACTCAGACAT ACATTCATGG GAATCGTTTC TTTCTGATGT TAGAGAAGAA 
ATTGAGAAAG CAAAGTCTCA GTTTGAAGAA CAAATTCAGG CAATTAAATA TGGTTCTCAG 
CTCAGTGAAC TTCCTAAAGT GCAGATTTCT GAGCTTTCAT TTCCTACCTG TAACACGATT 
CATCCTAAAT TACTTCCTGA GTCTTCAGGC CAGGATGGTC AAGGTCCCTC CACTTCTACA 
AGTCATGGGA CCGGAGACCA GACAGCAGCT CCCGAGGAGT CAAGTCTGGG CTCTGCAGCT 
GACAGCCCAG TGGAGTTGCC CTCCTGCAGT CAGAAAGCCC CCCAGCTGCC AGAGCATGAG 
GGTGCTGGCC AGGCAACTGG GCCAGAACCA GGCCCAGTGG CTGATCAGAA GTTACCTGCT 
GCTCCAGAGC GTGCCACTCA TTCAAGCCAG TCTCCAAAGA AGCCGTTCAA TAGCATTATT 
GAGCACCTGT TGGTGGTGTT CCCGTGTTAC AGCAGCACGG AGCTTGCTGG TTTTATTAAA 
AAAGTTAGAA ACAAAAACAA GACCTCTCTG TCTGGATTGA GTATTGACGA AATTGTGCAA 
AGAGTGACAG AACACGTCCT TGATGAAGAA AGAAAGAAAA AGCCAAGTCC AGGAAAGGGC 
TGGAGGCTGT CTGCACCCAG CTCTGCTGTT GCTGTGCCCA GGGCTGCCCA GGACCCGCCC 
TCCATGCCTG TCGGAGCATC GCCCGAAACC AGGGGGCCGA GGACCGAGGA CGTCCCTGTG 
TCCTGCGCAA ATTCCTGTGA AATGTGCCGA GAAGTATTCA AGTCCAAACA CGTGCGTGAG 
CTGCAGTGTG GGCACAGGCT TCACAAGGGG TGCTTCAAGC AGTGGCTGAA GAGCCAGCGC 
ACCTGCCCAG CCTGCCTGGG TCACGACCTC CTGTCAGAGG AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010799981.1
CDS	98..5362
Translation	MKMKGNEEFSKERYDIAIIYYTRAIECRPENHLLYGNRALCFLRTGQFRNALGDGKRATILKNNWTKGHYRYCAALSMLGEYDWALQANIKAQKLCKNDPEGIKDLIQQHVKLQKQIEDLQGQTPASNPVKAFCESRAYIPSLSAPAFSTSLNFVETEKNFRKTNHEMANGGNQNPKVVDEALKVDDCDCHPEFVPPSSQPPKYKGKQKSRNNELEKFSPGSQETLPVDLKNILEKQFSKSSRAAHQDFANIMKMLRSLIQDGYTALLEQRCRSAAQAFTELLNGLDPQKIKQLNLAMINYVLVVYGLAISLLGIGQPEELSEAENQFKRMIEHYPNEGLDCLAYCGIGKVYLKKNRFLEALNHFEKARTLICRLPGVLTWPTSNVVIEETQPGKIKMLLEKFVEECRFPPVPDATCCYQKCHGYSKIQIYITDPDFKGFIRISCCQYCKIEFHMNCWKKLKTTTFNDKIDKDFLQGICLTPDCEGIISKIIIFSSGGQVKCEFEHKVIKEKVPPRPILKQKCSSLEKLRLKEDKKLKRKLQKKEAKKLAQERLEEDLRESNPPKTEEQRETINHVQSCQFLDDRILQCIKQYADKIKSGILNTSKLLKELLSWKVLTTEDYTTCFSSRNFLNEAVDYVIRHLIQEKNRVKTRVFLHVLSELKEVDPKLATWIQKLNSFGLDATGPFFSRYGAPLKELDFSIMTFLWNEKYGHKLGSIEGKQLDYFYEPTSLKEARCLIWLLEEHRDKFPALHNALDEFFDIMDSRCTVLRKQDSGDVPFNSTKIKNKGKKKKPKDSKPMLVGSGTPSVTPNNETVTSGEDHIKRSLSSAGPFVVPDHLRQDIEEFEALYEQHSNEYVVRNKKLWDINPKQKCSTLYDYFSQLLEEHGPLDMSNKMFSEEYEFFPEETQQILEKAGGLKAFLLGCPRFVVIDNCIALKKVALRLKKKRKKKNIKTKVEEISKTGEYLRVKLPLNPTAREFKPDVKSKPVSDLPSAPASEDMKPQPAPANSPQSARDTGKPKPVFDNSSRSVSEDEKPKGMSSDSPRPVFEDTGHKPVSSNSPKPVPEEVKATYWPRPQLVTGYCTYLPFRGFDITQTPPAFISVLPTLPQYSIYTPLASVPTEYHLQRPIPVVPSFVASDITEVHFEAHHFSAENAPGTQIASETQILEESLETPVKSECSYDGADIVQSEASRNDDYCGDSAKWEVNPESTNEVTNIPCTQVVAVQVSWNITHQEVNTEPYGPFEAQQGGISQVEKEYQVLQEQLKEACENYEHSNVKGSEETKDLEEKLKRNVEENKISKTELDWFLEDLNKEIKKWEQEKKEIQERLKALKKKMKKVLNASKMYTQENDGKEKALELLLDQSLEISNTFTNEKMKIEESIKKGKEHYEESLQRAVDAEVSVLENWKDMEVCKLQSMESQAERFLKNLKLMSSDSAAYSDMDSDIHSWESFLSDVREEIEKAKSQFEEQIQAIKYGSQLSELPKVQISELSFPTCNTIHPKLLPESSGQDGQGPSTSTSHGTGDQTAAPEESSLGSAADSPVELPSCSQKAPQLPEHEGAGQATGPEPGPVADQKLPAAPERATHSSQSPKKPFNSIIEHLLVVFPCYSSTELAGFIKKVRNKNKTSLSGLSIDEIVQRVTEHVLDEERKKKPSPGKGWRLSAPSSAVAVPRAAQDPPSMPVGASPETRGPRTEDVPVSCANSCEMCREVFKSKHVRELQCGHRLHKGCFKQWLKSQRTCPACLGHDLLSEE

Target ORF information:

RefSeq Version	XM_010801679.2
Organism	Bos taurus(cattle)
Definition	Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010801679.2

ORF Insert Sequence:

ATGAAAATGA AAGGAAATGA AGAGTTTTCC AAAGAAAGAT ATGATATAGC TATTATCTAT 
TACACCAGAG CCATTGAATG TAGGCCTGAA AATCACCTGC TTTATGGTAA CCGAGCTCTT 
TGTTTTCTTC GTACTGGACA ATTCAGAAAT GCACTTGGTG ATGGAAAGAG AGCTACCATT 
CTGAAGAACA ATTGGACAAA GGGTCATTAT CGTTATTGTG CTGCTCTTTC TATGCTGGGG 
GAATATGACT GGGCCCTGCA AGCAAACATA AAAGCTCAAA AACTCTGTAA AAATGACCCT 
GAGGGAATCA AGGATCTAAT TCAGCAGCAT GTAAAGTTAC AAAAACAAAT AGAAGACCTG 
CAAGGTCAAA CACCAGCTAG TAATCCAGTC AAAGCCTTCT GTGAAAGCAG GGCCTACATA 
CCTAGTTTAT CAGCACCTGC ATTTAGTACT TCACTTAACT TTGTGGAAAC TGAAAAAAAT 
TTCAGAAAAA CTAACCACGA AATGGCAAAC GGTGGTAATC AGAATCCAAA GGTGGTAGAT 
GAAGCTCTGA AAGTAGATGA TTGTGACTGT CATCCTGAAT TTGTACCGCC ATCAAGTCAG 
CCTCCAAAAT ATAAAGGGAA ACAAAAATCT CGAAACAATG AATTGGAGAA GTTCAGCCCT 
GGTTCACAAG AAACTTTACC AGTAGATTTA AAAAACATCT TGGAGAAACA GTTTTCTAAA 
TCTTCCAGAG CCGCACACCA GGATTTTGCT AATATAATGA AAATGCTGAG AAGTTTAATT 
CAGGATGGCT ACACGGCCTT GCTGGAGCAG CGCTGCCGCA GCGCCGCCCA AGCCTTCACA 
GAGTTGCTCA ACGGTTTAGA TCCTCAAAAA ATAAAGCAAT TGAACCTGGC CATGATTAAC 
TATGTGTTAG TAGTCTACGG ACTTGCCATT TCACTCCTTG GAATAGGACA GCCCGAGGAG 
TTATCTGAAG CTGAAAACCA GTTCAAGAGG ATGATTGAAC ACTACCCCAA TGAGGGGCTT 
GATTGCTTGG CCTACTGTGG AATTGGAAAA GTGTATTTGA AAAAAAACAG ATTTTTAGAA 
GCTCTTAATC ATTTTGAGAA AGCAAGAACC TTGATCTGCC GTCTTCCTGG AGTGTTAACT 
TGGCCCACAA GTAATGTGGT TATTGAGGAG ACTCAGCCAG GGAAGATAAA GATGCTGTTA 
GAGAAATTTG TTGAAGAATG CAGGTTCCCT CCAGTGCCGG ATGCCACTTG TTGCTATCAG 
AAGTGCCATG GATATTCCAA AATCCAGATA TATATAACTG ATCCAGACTT TAAGGGTTTT 
ATACGGATCA GCTGTTGCCA GTATTGTAAA ATAGAATTTC ACATGAACTG CTGGAAGAAG 
TTAAAAACAA CAACCTTCAA TGATAAAATT GACAAGGATT TTCTGCAAGG AATCTGTCTT 
ACTCCTGATT GTGAAGGAAT CATTTCAAAG ATTATCATCT TCAGCAGTGG TGGTCAAGTT 
AAATGTGAAT TTGAACACAA GGTCATTAAA GAAAAGGTTC CTCCAAGACC TATTCTGAAA 
CAGAAATGTT CTAGCCTAGA GAAGTTGAGG CTGAAAGAAG ACAAAAAATT GAAGCGAAAG 
CTCCAAAAAA AGGAAGCAAA AAAATTAGCA CAAGAAAGAC TGGAAGAGGA CTTAAGGGAA 
AGTAACCCAC CAAAAACTGA AGAGCAGAGA GAAACTATCA ACCATGTCCA AAGTTGCCAG 
TTCCTTGATG ACAGAATTCT GCAGTGCATA AAGCAGTACG CTGACAAGAT TAAGTCCGGG 
ATACTGAATA CTTCCAAGCT TCTCAAAGAA TTGCTTTCTT GGAAGGTTTT AACCACAGAA 
GACTACACAA CGTGTTTTTC TAGCAGAAAT TTCCTGAATG AAGCAGTGGA CTATGTCATT 
CGTCATTTGA TCCAAGAAAA GAATAGAGTA AAGACAAGGG TATTTCTGCA TGTTTTGAGT 
GAGCTTAAAG AAGTGGACCC AAAACTAGCC ACCTGGATCC AGAAACTGAA TAGCTTTGGC 
TTAGATGCCA CAGGACCTTT CTTTTCTCGT TATGGGGCTC CTCTTAAGGA GCTTGATTTT 
AGCATCATGA CTTTTCTCTG GAATGAGAAA TACGGTCATA AGCTAGGCTC TATAGAAGGA 
AAGCAACTTG ATTATTTCTA TGAGCCTACA TCACTGAAGG AAGCGCGGTG TTTAATATGG 
CTGCTAGAAG AACACAGAGA CAAGTTCCCA GCACTGCATA ATGCCTTGGA CGAGTTCTTT 
GATATAATGG ACAGCCGCTG CACTGTATTA AGGAAACAGG ACAGTGGTGA CGTGCCATTT 
AATTCTACCA AGATAAAAAA CAAAGGCAAG AAAAAGAAGC CAAAAGATTC AAAGCCTATG 
TTAGTTGGAT CTGGAACACC TTCAGTCACA CCAAACAATG AGACTGTCAC TTCAGGTGAA 
GACCATATCA AACGCAGTTT AAGTTCTGCG GGCCCCTTTG TAGTGCCTGA TCACCTTCGG 
CAGGATATAG AAGAATTTGA AGCTCTGTAT GAACAGCATA GTAATGAGTA TGTTGTCCGT 
AATAAGAAGC TGTGGGACAT AAACCCAAAA CAGAAATGTT CAACTCTATA CGATTATTTC 
TCTCAGTTGT TGGAGGAACA TGGTCCCTTG GACATGAGTA ACAAAATGTT TTCTGAAGAA 
TATGAGTTCT TCCCAGAAGA AACTCAACAG ATACTAGAAA AAGCAGGAGG CTTGAAAGCG 
TTTCTCCTGG GATGTCCTCG TTTTGTTGTG ATTGATAACT GTATCGCATT GAAGAAAGTT 
GCATTACGGC TCAAGAAAAA GAGAAAGAAG AAAAACATTA AAACAAAAGT AGAAGAAATT 
TCAAAAACAG GAGAGTATTT ACGAGTTAAA CTACCACTGA ATCCAACTGC TAGGGAATTT 
AAACCAGATG TAAAGTCCAA ACCAGTGTCT GACTTACCTT CAGCACCAGC TTCTGAGGAC 
ATGAAGCCTC AGCCTGCACC TGCTAATTCT CCCCAATCAG CTCGTGACAC TGGGAAGCCA 
AAACCAGTTT TCGATAATTC TTCCAGATCA GTTTCTGAGG ATGAGAAGCC CAAAGGGATG 
TCTTCTGATT CTCCCAGGCC AGTCTTTGAG GATACCGGTC ACAAGCCGGT CTCCTCTAAT 
TCTCCCAAAC CAGTTCCTGA GGAAGTGAAA GCGACCTATT GGCCTCGACC CCAGTTGGTC 
ACAGGATACT GTACATATCT TCCCTTTCGG GGATTTGACA TTACCCAGAC TCCACCAGCC 
TTCATCAGTG TGTTACCGAC TTTGCCCCAG TATAGCATTT ACACCCCTCT GGCCAGTGTT 
CCTACTGAGT ATCACCTGCA AAGACCAATA CCAGTGGTGC CGTCTTTTGT AGCCAGTGAC 
ATAACGGAAG TGCATTTTGA GGCTCATCAT TTCAGTGCTG AGAATGCCCC TGGTACCCAG 
ATTGCCTCCG AAACACAGAT CCTTGAGGAA TCTTTGGAAA CACCAGTGAA GTCAGAGTGC 
AGCTATGATG GTGCTGATAT AGTCCAGAGT GAGGCTAGCA GAAACGATGA CTACTGTGGC 
GATTCTGCCA AATGGGAAGT AAATCCAGAA AGCACCAATG AAGTAACAAA TATTCCATGT 
ACACAAGTGG TTGCTGTACA GGTGTCTTGG AATATAACAC ATCAGGAAGT CAATACTGAG 
CCATATGGTC CTTTTGAGGC ACAACAGGGG GGTATTTCAC AGGTTGAAAA GGAGTACCAG 
GTTTTACAAG AGCAGCTTAA GGAAGCATGT GAAAATTATG AACACAGCAA TGTCAAGGGC 
TCAGAAGAAA CCAAGGACTT GGAAGAAAAG TTGAAAAGGA ATGTTGAAGA AAATAAGATC 
TCAAAGACAG AACTAGATTG GTTCCTTGAA GATTTGAACA AGGAAATTAA AAAATGGGAA 
CAGGAGAAAA AAGAAATCCA AGAAAGACTA AAGGCACTGA AGAAGAAAAT GAAAAAGGTG 
TTAAATGCCA GCAAAATGTA TACGCAGGAA AATGATGGAA AGGAAAAGGC ACTTGAATTA 
CTTCTGGATC AGTCCCTTGA AATCAGTAAT ACATTTACAA ATGAGAAAAT GAAAATAGAA 
GAGAGTATAA AGAAAGGGAA AGAGCATTAT GAAGAGAGTC TTCAAAGGGC TGTGGATGCA 
GAGGTATCTG TGCTTGAAAA CTGGAAGGAC ATGGAAGTGT GTAAGTTACA GAGCATGGAG 
TCACAAGCCG AAAGGTTTCT CAAGAATCTG AAGCTAATGA GCAGTGATTC GGCAGCGTAT 
TCTGACATGG ACTCAGACAT ACATTCATGG GAATCGTTTC TTTCTGATGT TAGAGAAGAA 
ATTGAGAAAG CAAAGTCTCA GTTTGAAGAA CAAATTCAGG CAATTAAATA TGGTTCTCAG 
CTCAGTGAAC TTCCTAAAGT GCAGATTTCT GAGCTTTCAT TTCCTACCTG TAACACGATT 
CATCCTAAAT TACTTCCTGA GTCTTCAGGC CAGGATGGTC AAGGTCCCTC CACTTCTACA 
AGTCATGGGA CCGGAGACCA GACAGCAGCT CCCGAGGAGT CAAGTCTGGG CTCTGCAGCT 
GACAGCCCAG TGGAGTTGCC CTCCTGCAGT CAGAAAGCCC CCCAGCTGCC AGAGCATGAG 
GGTGCTGGCC AGGCAACTGG GCCAGAACCA GGCCCAGTGG CTGATCAGAA GTTACCTGCT 
GCTCCAGAGC GTGCCACTCA TTCAAGCCAG TCTCCAAAGA AGCCGTTCAA TAGCATTATT 
GAGCACCTGT TGGTGGTGTT CCCGTGTTAC AGCAGCACGG AGCTTGCTGG TTTTATTAAA 
AAAGTTAGAA ACAAAAACAA GACCTCTCTG TCTGGATTGA GTATTGACGA AATTGTGCAA 
AGAGTGACAG AACACGTCCT TGATGAAGAA AGAAAGAAAA AGCCAAGTCC AGGAAAGGGC 
TGGAGGCTGT CTGCACCCAG CTCTGCTGTT GCTGTGCCCA GGGCTGCCCA GGACCCGCCC 
TCCATGCCTG TCGGAGCATC GCCCGAAACC AGGGGGCCGA GGACCGAGGA CGTCCCTGTG 
TCCTGCGCAA ATTCCTGTGA AATGTGCCGA GAAGTATTCA AGTCCAAACA CGTGCGTGAG 
CTGCAGTGTG GGCACAGGCT TCACAAGGGG TGCTTCAAGC AGTGGCTGAA GAGCCAGCGC 
ACCTGCCCAG CCTGCCTGGG TCACGACCTC CTGTCAGAGG AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_024999711.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855479.1 Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X1, mRNA.

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CloneID	OBa218652
Clone ID Related Accession (Same CDS sequence)	XM_024999711.1
Accession Version	XM_024999711.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6015bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase TTC3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACGAA AAAAAGCATT CTTGATGAAG GTGAAAAACA TCTGTGGGTG GTGTACCATG 
GCCGATTTTC CCCAGGGAAA TTTTGCAGAT TATGCCTTGT TAGAAGATTG CCCTTATGTG 
GATGATTGTA TCTTTGCCCC TGAGTTTATG TCCAGTGATT ATGTTCGTGT GACTCAGCTT 
TACTGTGATG GAGTGGGTAG ACAGTATAAA GATTATGCCC AAACTGAGGG AAACTTAGAA 
TTTGACATCT GCACTCTGTG GTGTAGTCAA CCTATTTCTG TCCTGCAAGA TTACTGTGAT 
GCCATTAAAG TCCACATCTT CTGGCCACTT CTGTTTCAAC GTCAGCAGAG CTCTATAATA 
ACACGGATGC ACTCCTGTGT AAGGACCAGC GGTTCTCATG CTTGTGAGAT AAGTTTGAAG 
AAATTACAGC AGCTTGAACT GATGGAAGAT ATTGTGGATT TGGCAAAGAA AGTTGTGAAT 
GATACATTCT TTATCGGAGG CTTATTGAGA ATTGGCTATA AAATAGAGAA TAAAATCTTG 
GCAATGGAAG AAGCTTTGAA TTGGGTGAAA TACACAGGCG ATGTAACAAT TTTATCTAAA 
TTAGGAGCAG TTGATAATTG TTGGCGTATG TTAAGTATTT TCTTTACTGA ATACAAGTAT 
CACATAACTA AAGTTGTAAC AGAAAACTGC AGTTTGCTTG AAGAATTTAA AACTCAAAGT 
TGTGCGGAGT GTCTAGAGCA AGGAGAACTA ATGAAAATGA AAGGAAATGA AGAGTTTTCC 
AAAGAAAGAT ATGATATAGC TATTATCTAT TACACCAGAG CCATTGAATG TAGGCCTGAA 
AATCACCTGC TTTATGGTAA CCGAGCTCTT TGTTTTCTTC GTACTGGACA ATTCAGAAAT 
GCACTTGGTG ATGGAAAGAG AGCTACCATT CTGAAGAACA ATTGGACAAA GGGTCATTAT 
CGTTATTGTG CTGCTCTTTC TATGCTGGGG GAATATGACT GGGCCCTGCA AGCAAACATA 
AAAGCTCAAA AACTCTGTAA AAATGACCCT GAGGGAATCA AGGATCTAAT TCAGCAGCAT 
GTAAAGTTAC AAAAACAAAT AGAAGACCTG CAAGGTCAAA CACCAGCTAG TAATCCAGTC 
AAAGCCTTCT GTGAAAGCAG GGCCTACATA CCTAGTTTAT CAGCACCTGC ATTTAGTACT 
TCACTTAACT TTGTGGAAAC TGAAAAAAAT TTCAGAAAAA CTAACCACGA AATGGCAAAC 
GGTGGTAATC AGAATCCAAA GGTGGTAGAT GAAGCTCTGA AAGTAGATGA TTGTGACTGT 
CATCCTGAAT TTGTACCGCC ATCAAGTCAG CCTCCAAAAT ATAAAGGGAA ACAAAAATCT 
CGAAACAATG AATTGGAGAA GTTCAGCCCT GGTTCACAAG AAACTTTACC AGTAGATTTA 
AAAAACATCT TGGAGAAACA GTTTTCTAAA TCTTCCAGAG CCGCACACCA GGATTTTGCT 
AATATAATGA AAATGTTGAG AAGTTTAATT CAGGATGGCT ACACGGCCTT GCTGGAGCAG 
CGCTGCCGCA GCGCCGCCCA AGCCTTCACA GAGTTGCTCA ACGGTTTAGA TCCTCAAAAA 
ATAAAGCAAT TAAACCTGGC CATGATTAAC TACGTGTTAG TAGTCTACGG ACTTGCCATT 
TCACTCCTTG GAATAGGACA GCCCGAGGAG TTATCTGAAG CTGAAAACCA GTTCAAGAGG 
ATGATTGAAC ACTACCCCAA TGAGGGGCTT GATTGCTTGG CCTACTGTGG AATTGGAAAA 
GTGTATTTGA AAAAAAACAG ATTTTTAGAA GCTCTTAATC ATTTTGAGAA AGCAAGAACC 
TTGATCTGTC GTCTTCCTGG AGTGTTAACT TGGCCCACAA GTAATGTGGT TATTGAGGAG 
ACTCAGCCAG GGAAGATAAA GATGCTGTTA GAGAAATTTG TTGAAGAATG CAGGTTCCCT 
CCAGTGCCGG ATGCCACTTG TTGCTATCAG AAGTGCCATG GATATTCCAA AATCCAGATA 
TATATAACTG ATCCAGACTT TAAGGGTTTT ATACGGATCA GCTGTTGCCA GTATTGTAAA 
ATAGAATTTC ACATGAACTG CTGGAAGAAG TTAAAAACAA CAACCTTCAA TGATAAAATT 
GACAAGGATT TTCTGCAAGG AATCTGTCTT ACTCCTGATT GTGAAGGAAT CATTTCAAAG 
ATTATCATCT TCAGCAGTGG TGGTCAAGTT AAATGTGAAT TTGAACACAA GGTCATTAAA 
GAAAAGGTTC CTCCAAGACC TATTCTGAAA CAGAAATGTT CTAGCCTAGA GAAGTTGAGG 
CTGAAAGAAG ACAAAAAATT GAAGCGAAAG CTCCAAAAAA AGGAAGCAAA AAAATTAGCA 
CAAGAAAGAC TGGAAGAGGA CTTAAGGGAA AGTAACCCAC CAAAAACTGA AGAGCAGAGA 
GAAACTATCA ACCATGTCCA AAGTTGCCAG TTCCTTGATG ACAGAATTCT GCAGTGCATA 
AAGCAGTACG CTGACAAGAT TAAGTCCGGG ATACTGAATA CTTCCAAGCT TCTCAAAGAA 
TTGCTTTCTT GGAAGGTTTT AACCACAGAA GACTACACAA CGTGTTTTTC TAGCAGAAAT 
TTCCTGAATG AAGCAGTGGA CTATGTCATT CGTCATTTGA TCCAAGAAAA GAATAGAGTA 
AAGACAAGGG TATTTCTGCA TGTTTTGAGT GAGCTTAAAG AAGTGGACCC AAAACTAGCC 
ACCTGGATCC AGAAACTGAA TAGCTTTGGC TTAGATGCCA CAGGACCTTT CTTTTCTCGT 
TATGGGGCTC CTCTTAAGGA GCTTGATTTT AGCATCATGA CTTTTCTCTG GAATGAGAAA 
TACGGTCATA AGCTAGGCTC TATAGAAGGA AAGCAACTTG ATTATTTCTA TGAGCCTACA 
TCACTGAAGG AAGCGCGGTG TTTAATATGG CTGCTAGAAG AACACAGAGA CAAGTTCCCA 
GCACTGCATA ATGCCTTGGA CGAGTTCTTT GATATAATGG ACAGCCGCTG CACTGTATTA 
AGGAAACAGG ACAGTGGTGA CGTGCCATTT AATTCTACCA AGATAAAAAA CAAAGGCAAG 
AAAAAGAAGC CAAAAGATTC AAAGCCTATG TTAGTTGGAT CTGGAACACC TTCAGTCACA 
CCAAACAATG AGACTGTCAC TTCAGGTGAA GACCATATCA AACGCAGTTT AAGTTCTGCG 
GGCCCCTTTG TAGTGCCTGA TCACCTTCGG CAGGATATAG AAGAATTTGA AGCTCTGTAT 
GAACAGCATA GTAATGAGTA TGTTGTCCGT AATAAGAAGC TGTGGGACAT AAACCCAAAA 
CAGAAATGTT CAACTCTATA CGATTATTTC TCTCAGTTGT TGGAGGAACA TGGTCCCTTG 
GACATGAGTA ACAAAATGTT TTCTGAAGAA TATGAGTTCT TCCCAGAAGA AACTCAACAG 
ATACTAGAAA AAGCAGGAGG CTTGAAAGCG TTTCTCCTGG GATGTCCTCG TTTTGTTGTG 
ATTGATAACT GTATCGCATT GAAGAAAGTT GCATTACGGC TCAAGAAAAA GAGAAAGAAG 
AAAAACATTA AAACAAAAGT AGAAGAAATT TCAAAAACAG GAGAGTATTT ACGAGTTAAA 
CTACCACTGA ATCCAACTGC TAGGGAATTT AAACCAGATG TAAAGTCCAA ACCAGTGTCT 
GACTTACCTT CAGCACCAGC TTCTGAGGAC ATGAAGCCTC AGCCTGCACC TGCTAATTCT 
CCCCAATCAG CTCGTGACAC TGGGAAGCCA AAACCAGTTT TCGATAATTC TTCCAGATCA 
GTTTCTGAGG ATGAGAAGCC CAAAGGGATG TCTTCTGATT CTCCCAGGCC AGTCTTTGAG 
GATACCGGTC ACAAGCCGGT CTCCTCTAAT TCTCCCAAAC CAGTTCCTGA GGAAGTGAAA 
GCGACCTATT GGCCTCGACC CCAGTTGGTC ACAGGATACT GTACATATCT TCCCTTTCGG 
GGATTTGACA TTACCCAGAC TCCACCAGCC TTCATCAGTG TGTTACCGAC TTTGCCCCAG 
TATAGCATTT ACACCCCTCT GGCCAGTGTT CCTACTGAGT ATCACCTGCA AAGACCAATA 
CCAGTGGTGC CGTCTTTTGT AGCCAGTGAC ATAACGGAAG TGCATTTTGA GGCTCATCAT 
TTCAGTGCTG AGAATGCCCC TGGTACCCAG ATTGCCTCCG AAACACAGAT CCTTGAGGAA 
TCTTTGGAAA CACCAGTGAA GTCAGAGTGC AGCTATGATG GTGCTGATAT AGTCCAGAGT 
GAGGCTAGCA GAAACGATGA CTACTGTGGC GATTCTGCCA AATGGGAAGT AAATCCAGAA 
AGCACCAATG AAGTAACAAA TATTCCATGT ACACAAGTGG TTGCTGTACA GGTGTCTTGG 
AATATAACAC ATCAGGAAGT CAATACTGAG CCATATGGTC CTTTTGAGGC ACAACAGGGG 
GGTATTTCAC AGGTTGAAAA GGAGTACCAG GTTTTACAAG AGCAGCTTAA GGAAGCATGT 
GAAAATTATG AACACAGCAA TGTCAAGGGC TCAGAAGAAA CCAAGGACTT GGAAGAAAAG 
TTGAAAAGGA ATGTTGAAGA AAATAAGATC TCAAAGACAG AACTAGATTG GTTCCTTGAA 
GATTTGAACA AGGAAATTAA AAAATGGGAA CAGGAGAAAA AAGAAATCCA AGAAAGACTA 
AAGGCACTGA AGAAGAAAAT GAAAAAGGTG TTAAATGCCA GCAAAATGTA TACGCAGGAA 
AATGATGGAA AGGAAAAGGC ACTTGAATTA CTTCTGGATC AGTCCCTTGA AATCAGTAAT 
ACATTTACAA ATGAGAAAAT GAAAATAGAA GAGAGTATAA AGAAAGGGAA AGAGCATTAT 
GAAGAGAGTC TTCAAAGGGC TGTGGATGCA GAGGTATCTG TGCTTGAAAA CTGGAAGGAC 
ATGGAAGTGT GTAAGTTACA GAGCATGGAG TCACAAGCCG AAAGGTTTCT CAAGAATCTG 
AAGCTAATGA GCAGTGATTC GGCAGCGTAT TCTGACATGG ACTCAGACAT ACATTCATGG 
GAATCGTTTC TTTCTGATGT TAGAGAAGAA ATTGAGAAAG CAAAGTCTCA GTTTGAAGAA 
CAAATTCAGG CAATTAAATA TGGTTCTCAG CTCAGTGAAC TTCCTAAAGT GCAGATTTCT 
GAGCTTTCAT TTCCTACCTG TAACACGATT CATCCTAAAT TACTTCCTGA GTCTTCAGGC 
CAGGATGGTC AAGGTCCCTC CACTTCTACA AGTCATGGGA CCGGAGACCA GACAGCAGCT 
CCCGAGGAGT CAAGTCTGGG CTCTGCAGCT GACAGCCCAG TGGAGTTGCC CTCCTGCAGT 
CAGAAAGCCC CCCAGCTGCC AGAGCATGAG GGTGCTGGCC AGGCAACTGG GCCAGAACCA 
GGCCCAGTGG CTGATCAGAA GTTACCTGCT GCTCCAGAGC GTGCCACTCA TTCAAGCCAG 
TCTCCAAAGA AGCCGTTCAA TAGCATTATT GAGCACCTGT TGGTGGTGTT CCCGTGTTAC 
AGCAGCACGG AGCTTGCTGG TTTTATTAAA AAAGTTAGAA ACAAAAACAA GACCTCTCTG 
TCTGGATTGA GTATTGACGA AATTGTGCAA AGAGTGACAG AACACGTCCT TGATGAAGAA 
AGAAAGAAAA AGCCAAGTCC AGGAAAGGGC TGGAGGCTGT CTGCACCCAG CTCTGCTGTT 
GCTGTGCCCA GGGCTGCCCA GGACCCGCCC TCCATGCCTG TCGGAGCATC GCCCGAAACC 
AGGGGGCCGA GGACCGAGGA CGTCCCTGTG TCCTGCGCAA ATTCCTGTGA AATGTGCCGA 
GAAGTATTCA AGTCCAAACA CGTGCGTGAG CTGCAGTGTG GGCACAGGCT TCACAAGGGG 
TGCTTCAAGC AGTGGCTGAA GAGCCAGCGC ACCTGCCCAG CCTGCCTGGG TCACGACCTC 
CTGTCAGAGG AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855479.1
CDS	2831..8845
Translation	MGRKKAFLMKVKNICGWCTMADFPQGNFADYALLEDCPYVDDCIFAPEFMSSDYVRVTQLYCDGVGRQYKDYAQTEGNLEFDICTLWCSQPISVLQDYCDAIKVHIFWPLLFQRQQSSIITRMHSCVRTSGSHACEISLKKLQQLELMEDIVDLAKKVVNDTFFIGGLLRIGYKIENKILAMEEALNWVKYTGDVTILSKLGAVDNCWRMLSIFFTEYKYHITKVVTENCSLLEEFKTQSCAECLEQGELMKMKGNEEFSKERYDIAIIYYTRAIECRPENHLLYGNRALCFLRTGQFRNALGDGKRATILKNNWTKGHYRYCAALSMLGEYDWALQANIKAQKLCKNDPEGIKDLIQQHVKLQKQIEDLQGQTPASNPVKAFCESRAYIPSLSAPAFSTSLNFVETEKNFRKTNHEMANGGNQNPKVVDEALKVDDCDCHPEFVPPSSQPPKYKGKQKSRNNELEKFSPGSQETLPVDLKNILEKQFSKSSRAAHQDFANIMKMLRSLIQDGYTALLEQRCRSAAQAFTELLNGLDPQKIKQLNLAMINYVLVVYGLAISLLGIGQPEELSEAENQFKRMIEHYPNEGLDCLAYCGIGKVYLKKNRFLEALNHFEKARTLICRLPGVLTWPTSNVVIEETQPGKIKMLLEKFVEECRFPPVPDATCCYQKCHGYSKIQIYITDPDFKGFIRISCCQYCKIEFHMNCWKKLKTTTFNDKIDKDFLQGICLTPDCEGIISKIIIFSSGGQVKCEFEHKVIKEKVPPRPILKQKCSSLEKLRLKEDKKLKRKLQKKEAKKLAQERLEEDLRESNPPKTEEQRETINHVQSCQFLDDRILQCIKQYADKIKSGILNTSKLLKELLSWKVLTTEDYTTCFSSRNFLNEAVDYVIRHLIQEKNRVKTRVFLHVLSELKEVDPKLATWIQKLNSFGLDATGPFFSRYGAPLKELDFSIMTFLWNEKYGHKLGSIEGKQLDYFYEPTSLKEARCLIWLLEEHRDKFPALHNALDEFFDIMDSRCTVLRKQDSGDVPFNSTKIKNKGKKKKPKDSKPMLVGSGTPSVTPNNETVTSGEDHIKRSLSSAGPFVVPDHLRQDIEEFEALYEQHSNEYVVRNKKLWDINPKQKCSTLYDYFSQLLEEHGPLDMSNKMFSEEYEFFPEETQQILEKAGGLKAFLLGCPRFVVIDNCIALKKVALRLKKKRKKKNIKTKVEEISKTGEYLRVKLPLNPTAREFKPDVKSKPVSDLPSAPASEDMKPQPAPANSPQSARDTGKPKPVFDNSSRSVSEDEKPKGMSSDSPRPVFEDTGHKPVSSNSPKPVPEEVKATYWPRPQLVTGYCTYLPFRGFDITQTPPAFISVLPTLPQYSIYTPLASVPTEYHLQRPIPVVPSFVASDITEVHFEAHHFSAENAPGTQIASETQILEESLETPVKSECSYDGADIVQSEASRNDDYCGDSAKWEVNPESTNEVTNIPCTQVVAVQVSWNITHQEVNTEPYGPFEAQQGGISQVEKEYQVLQEQLKEACENYEHSNVKGSEETKDLEEKLKRNVEENKISKTELDWFLEDLNKEIKKWEQEKKEIQERLKALKKKMKKVLNASKMYTQENDGKEKALELLLDQSLEISNTFTNEKMKIEESIKKGKEHYEESLQRAVDAEVSVLENWKDMEVCKLQSMESQAERFLKNLKLMSSDSAAYSDMDSDIHSWESFLSDVREEIEKAKSQFEEQIQAIKYGSQLSELPKVQISELSFPTCNTIHPKLLPESSGQDGQGPSTSTSHGTGDQTAAPEESSLGSAADSPVELPSCSQKAPQLPEHEGAGQATGPEPGPVADQKLPAAPERATHSSQSPKKPFNSIIEHLLVVFPCYSSTELAGFIKKVRNKNKTSLSGLSIDEIVQRVTEHVLDEERKKKPSPGKGWRLSAPSSAVAVPRAAQDPPSMPVGASPETRGPRTEDVPVSCANSCEMCREVFKSKHVRELQCGHRLHKGCFKQWLKSQRTCPACLGHDLLSEE

Target ORF information:

RefSeq Version	XM_024999711.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999711.1

ORF Insert Sequence:

ATGGGACGAA AAAAAGCATT CTTGATGAAG GTGAAAAACA TCTGTGGGTG GTGTACCATG 
GCCGATTTTC CCCAGGGAAA TTTTGCAGAT TATGCCTTGT TAGAAGATTG CCCTTATGTG 
GATGATTGTA TCTTTGCCCC TGAGTTTATG TCCAGTGATT ATGTTCGTGT GACTCAGCTT 
TACTGTGATG GAGTGGGTAG ACAGTATAAA GATTATGCCC AAACTGAGGG AAACTTAGAA 
TTTGACATCT GCACTCTGTG GTGTAGTCAA CCTATTTCTG TCCTGCAAGA TTACTGTGAT 
GCCATTAAAG TCCACATCTT CTGGCCACTT CTGTTTCAAC GTCAGCAGAG CTCTATAATA 
ACACGGATGC ACTCCTGTGT AAGGACCAGC GGTTCTCATG CTTGTGAGAT AAGTTTGAAG 
AAATTACAGC AGCTTGAACT GATGGAAGAT ATTGTGGATT TGGCAAAGAA AGTTGTGAAT 
GATACATTCT TTATCGGAGG CTTATTGAGA ATTGGCTATA AAATAGAGAA TAAAATCTTG 
GCAATGGAAG AAGCTTTGAA TTGGGTGAAA TACACAGGCG ATGTAACAAT TTTATCTAAA 
TTAGGAGCAG TTGATAATTG TTGGCGTATG TTAAGTATTT TCTTTACTGA ATACAAGTAT 
CACATAACTA AAGTTGTAAC AGAAAACTGC AGTTTGCTTG AAGAATTTAA AACTCAAAGT 
TGTGCGGAGT GTCTAGAGCA AGGAGAACTA ATGAAAATGA AAGGAAATGA AGAGTTTTCC 
AAAGAAAGAT ATGATATAGC TATTATCTAT TACACCAGAG CCATTGAATG TAGGCCTGAA 
AATCACCTGC TTTATGGTAA CCGAGCTCTT TGTTTTCTTC GTACTGGACA ATTCAGAAAT 
GCACTTGGTG ATGGAAAGAG AGCTACCATT CTGAAGAACA ATTGGACAAA GGGTCATTAT 
CGTTATTGTG CTGCTCTTTC TATGCTGGGG GAATATGACT GGGCCCTGCA AGCAAACATA 
AAAGCTCAAA AACTCTGTAA AAATGACCCT GAGGGAATCA AGGATCTAAT TCAGCAGCAT 
GTAAAGTTAC AAAAACAAAT AGAAGACCTG CAAGGTCAAA CACCAGCTAG TAATCCAGTC 
AAAGCCTTCT GTGAAAGCAG GGCCTACATA CCTAGTTTAT CAGCACCTGC ATTTAGTACT 
TCACTTAACT TTGTGGAAAC TGAAAAAAAT TTCAGAAAAA CTAACCACGA AATGGCAAAC 
GGTGGTAATC AGAATCCAAA GGTGGTAGAT GAAGCTCTGA AAGTAGATGA TTGTGACTGT 
CATCCTGAAT TTGTACCGCC ATCAAGTCAG CCTCCAAAAT ATAAAGGGAA ACAAAAATCT 
CGAAACAATG AATTGGAGAA GTTCAGCCCT GGTTCACAAG AAACTTTACC AGTAGATTTA 
AAAAACATCT TGGAGAAACA GTTTTCTAAA TCTTCCAGAG CCGCACACCA GGATTTTGCT 
AATATAATGA AAATGTTGAG AAGTTTAATT CAGGATGGCT ACACGGCCTT GCTGGAGCAG 
CGCTGCCGCA GCGCCGCCCA AGCCTTCACA GAGTTGCTCA ACGGTTTAGA TCCTCAAAAA 
ATAAAGCAAT TAAACCTGGC CATGATTAAC TACGTGTTAG TAGTCTACGG ACTTGCCATT 
TCACTCCTTG GAATAGGACA GCCCGAGGAG TTATCTGAAG CTGAAAACCA GTTCAAGAGG 
ATGATTGAAC ACTACCCCAA TGAGGGGCTT GATTGCTTGG CCTACTGTGG AATTGGAAAA 
GTGTATTTGA AAAAAAACAG ATTTTTAGAA GCTCTTAATC ATTTTGAGAA AGCAAGAACC 
TTGATCTGTC GTCTTCCTGG AGTGTTAACT TGGCCCACAA GTAATGTGGT TATTGAGGAG 
ACTCAGCCAG GGAAGATAAA GATGCTGTTA GAGAAATTTG TTGAAGAATG CAGGTTCCCT 
CCAGTGCCGG ATGCCACTTG TTGCTATCAG AAGTGCCATG GATATTCCAA AATCCAGATA 
TATATAACTG ATCCAGACTT TAAGGGTTTT ATACGGATCA GCTGTTGCCA GTATTGTAAA 
ATAGAATTTC ACATGAACTG CTGGAAGAAG TTAAAAACAA CAACCTTCAA TGATAAAATT 
GACAAGGATT TTCTGCAAGG AATCTGTCTT ACTCCTGATT GTGAAGGAAT CATTTCAAAG 
ATTATCATCT TCAGCAGTGG TGGTCAAGTT AAATGTGAAT TTGAACACAA GGTCATTAAA 
GAAAAGGTTC CTCCAAGACC TATTCTGAAA CAGAAATGTT CTAGCCTAGA GAAGTTGAGG 
CTGAAAGAAG ACAAAAAATT GAAGCGAAAG CTCCAAAAAA AGGAAGCAAA AAAATTAGCA 
CAAGAAAGAC TGGAAGAGGA CTTAAGGGAA AGTAACCCAC CAAAAACTGA AGAGCAGAGA 
GAAACTATCA ACCATGTCCA AAGTTGCCAG TTCCTTGATG ACAGAATTCT GCAGTGCATA 
AAGCAGTACG CTGACAAGAT TAAGTCCGGG ATACTGAATA CTTCCAAGCT TCTCAAAGAA 
TTGCTTTCTT GGAAGGTTTT AACCACAGAA GACTACACAA CGTGTTTTTC TAGCAGAAAT 
TTCCTGAATG AAGCAGTGGA CTATGTCATT CGTCATTTGA TCCAAGAAAA GAATAGAGTA 
AAGACAAGGG TATTTCTGCA TGTTTTGAGT GAGCTTAAAG AAGTGGACCC AAAACTAGCC 
ACCTGGATCC AGAAACTGAA TAGCTTTGGC TTAGATGCCA CAGGACCTTT CTTTTCTCGT 
TATGGGGCTC CTCTTAAGGA GCTTGATTTT AGCATCATGA CTTTTCTCTG GAATGAGAAA 
TACGGTCATA AGCTAGGCTC TATAGAAGGA AAGCAACTTG ATTATTTCTA TGAGCCTACA 
TCACTGAAGG AAGCGCGGTG TTTAATATGG CTGCTAGAAG AACACAGAGA CAAGTTCCCA 
GCACTGCATA ATGCCTTGGA CGAGTTCTTT GATATAATGG ACAGCCGCTG CACTGTATTA 
AGGAAACAGG ACAGTGGTGA CGTGCCATTT AATTCTACCA AGATAAAAAA CAAAGGCAAG 
AAAAAGAAGC CAAAAGATTC AAAGCCTATG TTAGTTGGAT CTGGAACACC TTCAGTCACA 
CCAAACAATG AGACTGTCAC TTCAGGTGAA GACCATATCA AACGCAGTTT AAGTTCTGCG 
GGCCCCTTTG TAGTGCCTGA TCACCTTCGG CAGGATATAG AAGAATTTGA AGCTCTGTAT 
GAACAGCATA GTAATGAGTA TGTTGTCCGT AATAAGAAGC TGTGGGACAT AAACCCAAAA 
CAGAAATGTT CAACTCTATA CGATTATTTC TCTCAGTTGT TGGAGGAACA TGGTCCCTTG 
GACATGAGTA ACAAAATGTT TTCTGAAGAA TATGAGTTCT TCCCAGAAGA AACTCAACAG 
ATACTAGAAA AAGCAGGAGG CTTGAAAGCG TTTCTCCTGG GATGTCCTCG TTTTGTTGTG 
ATTGATAACT GTATCGCATT GAAGAAAGTT GCATTACGGC TCAAGAAAAA GAGAAAGAAG 
AAAAACATTA AAACAAAAGT AGAAGAAATT TCAAAAACAG GAGAGTATTT ACGAGTTAAA 
CTACCACTGA ATCCAACTGC TAGGGAATTT AAACCAGATG TAAAGTCCAA ACCAGTGTCT 
GACTTACCTT CAGCACCAGC TTCTGAGGAC ATGAAGCCTC AGCCTGCACC TGCTAATTCT 
CCCCAATCAG CTCGTGACAC TGGGAAGCCA AAACCAGTTT TCGATAATTC TTCCAGATCA 
GTTTCTGAGG ATGAGAAGCC CAAAGGGATG TCTTCTGATT CTCCCAGGCC AGTCTTTGAG 
GATACCGGTC ACAAGCCGGT CTCCTCTAAT TCTCCCAAAC CAGTTCCTGA GGAAGTGAAA 
GCGACCTATT GGCCTCGACC CCAGTTGGTC ACAGGATACT GTACATATCT TCCCTTTCGG 
GGATTTGACA TTACCCAGAC TCCACCAGCC TTCATCAGTG TGTTACCGAC TTTGCCCCAG 
TATAGCATTT ACACCCCTCT GGCCAGTGTT CCTACTGAGT ATCACCTGCA AAGACCAATA 
CCAGTGGTGC CGTCTTTTGT AGCCAGTGAC ATAACGGAAG TGCATTTTGA GGCTCATCAT 
TTCAGTGCTG AGAATGCCCC TGGTACCCAG ATTGCCTCCG AAACACAGAT CCTTGAGGAA 
TCTTTGGAAA CACCAGTGAA GTCAGAGTGC AGCTATGATG GTGCTGATAT AGTCCAGAGT 
GAGGCTAGCA GAAACGATGA CTACTGTGGC GATTCTGCCA AATGGGAAGT AAATCCAGAA 
AGCACCAATG AAGTAACAAA TATTCCATGT ACACAAGTGG TTGCTGTACA GGTGTCTTGG 
AATATAACAC ATCAGGAAGT CAATACTGAG CCATATGGTC CTTTTGAGGC ACAACAGGGG 
GGTATTTCAC AGGTTGAAAA GGAGTACCAG GTTTTACAAG AGCAGCTTAA GGAAGCATGT 
GAAAATTATG AACACAGCAA TGTCAAGGGC TCAGAAGAAA CCAAGGACTT GGAAGAAAAG 
TTGAAAAGGA ATGTTGAAGA AAATAAGATC TCAAAGACAG AACTAGATTG GTTCCTTGAA 
GATTTGAACA AGGAAATTAA AAAATGGGAA CAGGAGAAAA AAGAAATCCA AGAAAGACTA 
AAGGCACTGA AGAAGAAAAT GAAAAAGGTG TTAAATGCCA GCAAAATGTA TACGCAGGAA 
AATGATGGAA AGGAAAAGGC ACTTGAATTA CTTCTGGATC AGTCCCTTGA AATCAGTAAT 
ACATTTACAA ATGAGAAAAT GAAAATAGAA GAGAGTATAA AGAAAGGGAA AGAGCATTAT 
GAAGAGAGTC TTCAAAGGGC TGTGGATGCA GAGGTATCTG TGCTTGAAAA CTGGAAGGAC 
ATGGAAGTGT GTAAGTTACA GAGCATGGAG TCACAAGCCG AAAGGTTTCT CAAGAATCTG 
AAGCTAATGA GCAGTGATTC GGCAGCGTAT TCTGACATGG ACTCAGACAT ACATTCATGG 
GAATCGTTTC TTTCTGATGT TAGAGAAGAA ATTGAGAAAG CAAAGTCTCA GTTTGAAGAA 
CAAATTCAGG CAATTAAATA TGGTTCTCAG CTCAGTGAAC TTCCTAAAGT GCAGATTTCT 
GAGCTTTCAT TTCCTACCTG TAACACGATT CATCCTAAAT TACTTCCTGA GTCTTCAGGC 
CAGGATGGTC AAGGTCCCTC CACTTCTACA AGTCATGGGA CCGGAGACCA GACAGCAGCT 
CCCGAGGAGT CAAGTCTGGG CTCTGCAGCT GACAGCCCAG TGGAGTTGCC CTCCTGCAGT 
CAGAAAGCCC CCCAGCTGCC AGAGCATGAG GGTGCTGGCC AGGCAACTGG GCCAGAACCA 
GGCCCAGTGG CTGATCAGAA GTTACCTGCT GCTCCAGAGC GTGCCACTCA TTCAAGCCAG 
TCTCCAAAGA AGCCGTTCAA TAGCATTATT GAGCACCTGT TGGTGGTGTT CCCGTGTTAC 
AGCAGCACGG AGCTTGCTGG TTTTATTAAA AAAGTTAGAA ACAAAAACAA GACCTCTCTG 
TCTGGATTGA GTATTGACGA AATTGTGCAA AGAGTGACAG AACACGTCCT TGATGAAGAA 
AGAAAGAAAA AGCCAAGTCC AGGAAAGGGC TGGAGGCTGT CTGCACCCAG CTCTGCTGTT 
GCTGTGCCCA GGGCTGCCCA GGACCCGCCC TCCATGCCTG TCGGAGCATC GCCCGAAACC 
AGGGGGCCGA GGACCGAGGA CGTCCCTGTG TCCTGCGCAA ATTCCTGTGA AATGTGCCGA 
GAAGTATTCA AGTCCAAACA CGTGCGTGAG CTGCAGTGTG GGCACAGGCT TCACAAGGGG 
TGCTTCAAGC AGTGGCTGAA GAGCCAGCGC ACCTGCCCAG CCTGCCTGGG TCACGACCTC 
CTGTCAGAGG AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	TTC3
Entrez Gene ID	506569
Full Name	tetratricopeptide repeat domain 3
General protein information	Preferred Names tetratricopeptide repeat domain 3 Names E3 ubiquitin-protein ligase TTC3
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Rattus norvegicus (Norway rat)	Ttc3	NP_001101785.1
Bos taurus (cattle)	TTC3	NP_001103237.1
Xenopus tropicalis (tropical clawed frog)	ttc3	XP_002941578.2
	TTC3	XP_001098049.2
Gallus gallus (chicken)	TTC3	XP_416727.4
Danio rerio (zebrafish)	LOC100329450	XP_005172636.1
Pan troglodytes (chimpanzee)	TTC3	XP_001169932.1
Canis lupus familiaris (dog)	TTC3	XP_849567.2
Mus musculus (house mouse)	Ttc3	NP_033467.2
Homo sapiens (human)	TTC3	NP_001001894.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

TTC3 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

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