PHLDA2 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Gene Symbol	PHLDA2
Entrez Gene ID	618810
Full Name	pleckstrin homology like domain family A member 2
General protein information	Preferred Names pleckstrin homology like domain family A member 2 Names pleckstrin homology-like domain family A member 2 imprinted in placenta and liver protein
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA Protein Name

NM_001076521.2 NP_001069989.1 pleckstrin homology-like domain family A member 2
Pathways from BioSystems

mRNA	Protein	Name
NM_001076521.2	NP_001069989.1	pleckstrin homology-like domain family A member 2

Homologies

Danio rerio (zebrafish)	phlda2	NP_001018432.1
Xenopus tropicalis (tropical clawed frog)	phlda2	NP_001072825.1
Homo sapiens (human)	PHLDA2	NP_003302.1
	PHLDA2	XP_001094620.1
Gallus gallus (chicken)	PHLDA2	NP_001186524.1
Rattus norvegicus (Norway rat)	Phlda2	NP_001093991.1
Pan troglodytes (chimpanzee)	PHLDA2	XP_001155149.1
Bos taurus (cattle)	PHLDA2	NP_001069989.1
Mus musculus (house mouse)	Phlda2	NP_033460.1

Gene Ontology
Bibliography

Related articles in PubMed

Nuclear transfer alters placental gene expression and associated histone modifications of the placental-specific imprinted gene pleckstrin homology-like domain, family A, member 2 (PHLDA2) in cattle.
Arnold DR, Gaspar RC, da Rocha CV, Sangalli JR, de Bem THC, Corr?a CAP, Penteado JCT, Meirelles FV, Lopes FL
Reproduction, fertility, and development29(3)458-467(2017 Mar)

Biallelic expression of Tssc4, Nap1l4, Phlda2 and Osbpl5 in adult cattle.
Wang M, Li D, Zhang M, Yang W, Wu G, Cui Y, Li S
Journal of genetics94(3)391-5(2015 Sep)

Knockdown of CDKN1C (p57(kip2)) and PHLDA2 results in developmental changes in bovine pre-implantation embryos.
Driver AM, Huang W, Kropp J, Pe?agaricano F, Khatib H
PloS one8(7)e69490(2013)

PHLDA2 is an imprinted gene in cattle.
Sikora KM, Magee DA, Berkowicz EW, Lonergan P, Evans AC, Carter F, Comte A, Waters SM, MacHugh DE, Spillane C
Animal genetics43(5)587-90(2012 Oct)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

imprinting status of four cattle genes (Tssc4,Nap1l4, Phlda2 and Osbpl5) in seven types of tissues, were assessed in cattle.
Title: Biallelic expression of Tssc4, Nap1l4, Phlda2 and Osbpl5 in adult cattle.

proper expression levels of the imprinted genes CDKN1C and PHLDA2 are critical for embryo development
Title: Knockdown of CDKN1C (p57(kip2)) and PHLDA2 results in developmental changes in bovine pre-implantation embryos.

Pleckstrin homology-like domain family A member 2 (PHLDA2) gene is expressed across a range of cattle fetal tissues and stages. It provides the first evidence that PHLDA2 is a monoallelically expressed imprinted gene in cattle fetal tissues and placenta.
Title: PHLDA2 is an imprinted gene in cattle.

The following PHLDA2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PHLDA2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa01309	NM_001076521.2 Latest version!	Bos taurus pleckstrin homology like domain family A member 2 (PHLDA2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$104.30 $149.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PHLDA2 ( NM_001076521.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001069989.1 Bos taurus pleckstrin homology like domain family A member 2 (PHLDA2), mRNA.

Price & Availability↑

CloneID	OBa01309
Clone ID Related Accession (Same CDS sequence)	NM_001076521.2
Accession Version	NM_001076521.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Bos taurus(cattle)
Product	pleckstrin homology-like domain family A member 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC120298.1. On Aug 30, 2012 this sequence version replaced NM_001076521.1. ##Evidence-Data-START## Transcript exon combination :: BC120298.1, EE253159.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145418 [ECO:0000348] ##Evidence-Data-END##

ATGAGGAGCC CGGGAGAGGT GCTGAGAGAG GGCGAGCTGG AGAAGCGAAG TGACAGCCTG 
CTCCAGGTGT GGAAGAAGAA GCGCGGCGTG CTCACCACCG ACCGCCTGAG GCTGTTCCCC 
ACCGGCCCCG GCGCACGCCC CAAGGAGCTG CGTTTCCACT CCATCCTCAA GGTGGACTGC 
GTGGAGCGCA CCGGCAAGTA CGTCTACTTC ACCATCGTCA CCACCGACCG CAAGGAGATC 
GACTTCCGCT GCGCGGGCGA GAGCTACTGG AACGCGTCCA TCACGCTGGC GCTCATCGAC 
TTTCAGAACC GCCGCGCCAT GCAGGACTTC CGCAGCCGCC GCGAGCGCGC CGCGGCCGCC 
GCGGCTGCAG CGGCCGCCGC GGCCGCCGCG GAGCAGGAGC CCGAGGCCGG GCCGGGCGGC 
CAGCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001069989.1
CDS	63..491
Translation	MRSPGEVLREGELEKRSDSLLQVWKKKRGVLTTDRLRLFPTGPGARPKELRFHSILKVDCVERTGKYVYFTIVTTDRKEIDFRCAGESYWNASITLALIDFQNRRAMQDFRSRRERAAAAAAAAAAAAAAEQEPEAGPGGQP

Target ORF information:

RefSeq Version	NM_001076521.2
Organism	Bos taurus(cattle)
Definition	Bos taurus pleckstrin homology like domain family A member 2 (PHLDA2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001076521.2

ORF Insert Sequence:

ATGAGGAGCC CGGGAGAGGT GCTGAGAGAG GGCGAGCTGG AGAAGCGAAG TGACAGCCTG 
CTCCAGGTGT GGAAGAAGAA GCGCGGCGTG CTCACCACCG ACCGCCTGAG GCTGTTCCCC 
ACCGGCCCCG GCGCACGCCC CAAGGAGCTG CGTTTCCACT CCATCCTCAA GGTGGACTGC 
GTGGAGCGCA CCGGCAAGTA CGTCTACTTC ACCATCGTCA CCACCGACCG CAAGGAGATC 
GACTTCCGCT GCGCGGGCGA GAGCTACTGG AACGCGTCCA TCACGCTGGC GCTCATCGAC 
TTTCAGAACC GCCGCGCCAT GCAGGACTTC CGCAGCCGCC GCGAGCGCGC CGCGGCCGCC 
GCGGCTGCAG CGGCCGCCGC GGCCGCCGCG GAGCAGGAGC CCGAGGCCGG GCCGGGCGGC 
CAGCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Nuclear transfer alters placental gene expression and associated histone modifications of the placental-specific imprinted gene pleckstrin homology-like domain, family A, member 2 (PHLDA2) in cattle.
Reproduction, fertility, and development29(3)458-467(2017 Mar)
Arnold DR,Gaspar RC,da Rocha CV,Sangalli JR,de Bem THC,Corr?a CAP,Penteado JCT,Meirelles FV,Lopes FL

Biallelic expression of Tssc4, Nap1l4, Phlda2 and Osbpl5 in adult cattle.
Journal of genetics94(3)391-5(2015 Sep)
Wang M,Li D,Zhang M,Yang W,Wu G,Cui Y,Li S

Knockdown of CDKN1C (p57(kip2)) and PHLDA2 results in developmental changes in bovine pre-implantation embryos.
PloS one8(7)e69490(2013)
Driver AM,Huang W,Kropp J,Pe?agaricano F,Khatib H

PHLDA2 is an imprinted gene in cattle.
Animal genetics43(5)587-90(2012 Oct)
Sikora KM,Magee DA,Berkowicz EW,Lonergan P,Evans AC,Carter F,Comte A,Waters SM,MacHugh DE,Spillane C

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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PHLDA2 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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