PPA1 cDNA ORF clone, Calidris pugnax(ruff)

Gene
Clones
gRNAs

Summary

Gene Symbol	PPA1
Entrez Gene ID	106886989
Full Name	pyrophosphatase (inorganic) 1
Gene Type	protein-coding
Organism	Calidris pugnax(ruff)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014940326.1	XP_014795812.1	inorganic pyrophosphatase

Pathways from BioSystems
Gene Ontology

The following PPA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PPA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCa63830	XM_014940326.1 Latest version!	Calidris pugnax pyrophosphatase (inorganic) 1 (PPA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PPA1 ( XM_014940326.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014795812.1 Calidris pugnax pyrophosphatase (inorganic) 1 (PPA1), mRNA.

Price & Availability↑

CloneID	OCa63830
Clone ID Related Accession (Same CDS sequence)	XM_014940326.1
Accession Version	XM_014940326.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	inorganic pyrophosphatase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090828.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGTGG AAGTACCTCG ATGGACAAAC GCGAAGATGG AGATTGCAAC AAAGGATCCC 
TTAAACCCAA TTAAGCAAGA CGTTAAGAAA GGAAAACTGC GCTACGTAGC GAACGTGTTT 
CCCCATAAAG GTTATATCTG GAATTACGGT GCTATCCCAC AGACTTGGGA AGATCCAGCT 
CACAAAGATG AAAACACTGG CTGCTGTGGA GATAACGATC CGATTGACGT GTGTGAAATT 
GGAAGCAAGG TCTGCTCTCG AGGAGAAATC ATCAAGGTGA AGGTGCTGGG CACGCTGGCT 
CTGATTGATG AGGGGGAGAC AGACTGGAAG ATAATTGCTA TCAATGTTGA AGACCCTGAA 
GCAGGCAACT TTAATGATAT CAACGATGTC CGAAGGCTGA AACCTGGATA CCTGGAAGCT 
ACAGTGGACT GGTTCAGAAG ATACAAAGTA CCTGATGGAA AGCCAGAAAA CCAGTTTGCT 
TTTAATGGTG AATTTAAAGA CAAGGATTTT GCGGTGAATG TCATCAAAAA CACTCATGAA 
CACTGGAAAG CTTTAATAGC AAAGAAGACT GACGGTGGGG AGATCAACTG CACAAACCTG 
ACGGTGTCCG ACAGCCCTTT CTGCTGTAGC CAAGAGTGTG CAAAAGCTAC TGTGGATGCA 
GCACCGCCGA GTAAAGCTGC CAACCCCATC CCGCCCGAAG TTGACAAGTG GTTCTACTAC 
CAGAAGAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014795812.1
CDS	31..762
Translation	MVVEVPRWTNAKMEIATKDPLNPIKQDVKKGKLRYVANVFPHKGYIWNYGAIPQTWEDPAHKDENTGCCGDNDPIDVCEIGSKVCSRGEIIKVKVLGTLALIDEGETDWKIIAINVEDPEAGNFNDINDVRRLKPGYLEATVDWFRRYKVPDGKPENQFAFNGEFKDKDFAVNVIKNTHEHWKALIAKKTDGGEINCTNLTVSDSPFCCSQECAKATVDAAPPSKAANPIPPEVDKWFYYQKN

Target ORF information:

RefSeq Version	XM_014940326.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax pyrophosphatase (inorganic) 1 (PPA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014940326.1

ORF Insert Sequence:

ATGGTGGTGG AAGTACCTCG ATGGACAAAC GCGAAGATGG AGATTGCAAC AAAGGATCCC 
TTAAACCCAA TTAAGCAAGA CGTTAAGAAA GGAAAACTGC GCTACGTAGC GAACGTGTTT 
CCCCATAAAG GTTATATCTG GAATTACGGT GCTATCCCAC AGACTTGGGA AGATCCAGCT 
CACAAAGATG AAAACACTGG CTGCTGTGGA GATAACGATC CGATTGACGT GTGTGAAATT 
GGAAGCAAGG TCTGCTCTCG AGGAGAAATC ATCAAGGTGA AGGTGCTGGG CACGCTGGCT 
CTGATTGATG AGGGGGAGAC AGACTGGAAG ATAATTGCTA TCAATGTTGA AGACCCTGAA 
GCAGGCAACT TTAATGATAT CAACGATGTC CGAAGGCTGA AACCTGGATA CCTGGAAGCT 
ACAGTGGACT GGTTCAGAAG ATACAAAGTA CCTGATGGAA AGCCAGAAAA CCAGTTTGCT 
TTTAATGGTG AATTTAAAGA CAAGGATTTT GCGGTGAATG TCATCAAAAA CACTCATGAA 
CACTGGAAAG CTTTAATAGC AAAGAAGACT GACGGTGGGG AGATCAACTG CACAAACCTG 
ACGGTGTCCG ACAGCCCTTT CTGCTGTAGC CAAGAGTGTG CAAAAGCTAC TGTGGATGCA 
GCACCGCCGA GTAAAGCTGC CAACCCCATC CCGCCCGAAG TTGACAAGTG GTTCTACTAC 
CAGAAGAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.