SCRIB gene cDNA ORF clone, Calidris pugnax(ruff)

mRNA	Protein	Name
XM_014945733.1	XP_014801219.1	protein scribble homolog isoform X19
XM_014945716.1	XP_014801202.1	protein scribble homolog isoform X3
XM_014945722.1	XP_014801208.1	protein scribble homolog isoform X9
XM_014945725.1	XP_014801211.1	protein scribble homolog isoform X11
XM_014945726.1	XP_014801212.1	protein scribble homolog isoform X12
XM_014945717.1	XP_014801203.1	protein scribble homolog isoform X4
XM_014945718.1	XP_014801204.1	protein scribble homolog isoform X5
XM_014945724.1	XP_014801210.1	protein scribble homolog isoform X10
XM_014945729.1	XP_014801215.1	protein scribble homolog isoform X15
XM_014945714.1	XP_014801200.1	protein scribble homolog isoform X1
XM_014945719.1	XP_014801205.1	protein scribble homolog isoform X6
XM_014945720.1	XP_014801206.1	protein scribble homolog isoform X7
XM_014945721.1	XP_014801207.1	protein scribble homolog isoform X8
XM_014945728.1	XP_014801214.1	protein scribble homolog isoform X14
XM_014945731.1	XP_014801217.1	protein scribble homolog isoform X17
XM_014945732.1	XP_014801218.1	protein scribble homolog isoform X18
XM_014945734.1	XP_014801220.1	protein scribble homolog isoform X20
XM_014945715.1	XP_014801201.1	protein scribble homolog isoform X2
XM_014945727.1	XP_014801213.1	protein scribble homolog isoform X13
XM_014945730.1	XP_014801216.1	protein scribble homolog isoform X16

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa67747	XM_014945714.1 Latest version!	Calidris pugnax scribbled planar cell polarity protein (SCRIB), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCa67748	XM_014945715.1 Latest version!	Calidris pugnax scribbled planar cell polarity protein (SCRIB), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCa67749	XM_014945716.1 Latest version!	Calidris pugnax scribbled planar cell polarity protein (SCRIB), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCa67750	XM_014945717.1 Latest version!	Calidris pugnax scribbled planar cell polarity protein (SCRIB), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCa67751	XM_014945718.1 Latest version!	Calidris pugnax scribbled planar cell polarity protein (SCRIB), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCa67752	XM_014945719.1 Latest version!	Calidris pugnax scribbled planar cell polarity protein (SCRIB), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCa67753	XM_014945720.1 Latest version!	Calidris pugnax scribbled planar cell polarity protein (SCRIB), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCa67754	XM_014945721.1 Latest version!	Calidris pugnax scribbled planar cell polarity protein (SCRIB), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCa67755	XM_014945722.1 Latest version!	Calidris pugnax scribbled planar cell polarity protein (SCRIB), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCa67756	XM_014945724.1 Latest version!	Calidris pugnax scribbled planar cell polarity protein (SCRIB), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCa67757	XM_014945725.1 Latest version!	Calidris pugnax scribbled planar cell polarity protein (SCRIB), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCa67758	XM_014945726.1 Latest version!	Calidris pugnax scribbled planar cell polarity protein (SCRIB), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCa67759	XM_014945727.1 Latest version!	Calidris pugnax scribbled planar cell polarity protein (SCRIB), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCa67760	XM_014945728.1 Latest version!	Calidris pugnax scribbled planar cell polarity protein (SCRIB), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCa67761	XM_014945729.1 Latest version!	Calidris pugnax scribbled planar cell polarity protein (SCRIB), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCa67762	XM_014945730.1 Latest version!	Calidris pugnax scribbled planar cell polarity protein (SCRIB), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCa67763	XM_014945731.1 Latest version!	Calidris pugnax scribbled planar cell polarity protein (SCRIB), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCa67764	XM_014945732.1 Latest version!	Calidris pugnax scribbled planar cell polarity protein (SCRIB), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCa67765	XM_014945733.1 Latest version!	Calidris pugnax scribbled planar cell polarity protein (SCRIB), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCa67766	XM_014945734.1 Latest version!	Calidris pugnax scribbled planar cell polarity protein (SCRIB), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00

SCRIB ( XM_014945714.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014801200.1 Calidris pugnax scribbled planar cell polarity protein (SCRIB), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCa67747
Clone ID Related Accession (Same CDS sequence)	XM_014945714.1
Accession Version	XM_014945714.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5961bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	protein scribble homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090871.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCCGC TCTGGCGCTG CAACCGGCAC GTGGAGGCGC TGGACCGGCG GCACTGCTCG 
CTGCAGGCCG TGCCCGAGGA GATCTACCGC TACAGCCGCT CGCTGGAGGA GCTGCTGCTG 
GACGCCAACC AGCTGCGGGA GCTGCCCAAG CCTTTCTTCA GGCTTCTGAA CCTGCGCAAG 
CTGGGCCTGA GTGACAATGA GATCCAAAGA CTTCCCCCTG AGGTGGCCAA CTTCATGCAG 
CTGGTGGAAC TGGACATCTC CCGTAACGAC ATTCCAGAAA TTCCTGAAAG CATCAAATTC 
TGCAAATCCT TGGAAATCGC AGACTTCAGT GGGAATCCTC TCTCCAGGCT TCCCGAGGGC 
TTCACGCAGC TTCGGAGCCT GGGCCATTTG GCCCTGAATG ATGTGTCCCT GCAGAGCCTC 
CCCAGTGACA TTGGGAATTT GGCAAACCTG GTGACTCTGG AGCTACGCGA GAACCTGCTA 
AAGACTCTCC CCACCTCGCT GTCCTTCCTT GTCAAGTTAG AGCAGCTGGA TCTCGGTGGC 
AATGACCTGG AGGTTCTGCC AGACACTCTG GGAGCGCTCC CCAACCTGCG TGAGCTCTGG 
TTAGACCGGA ACCAGCTCTC AGCCCTGCCT CCCGAGCTGG GCAACCTCCG CCGCCTGGTC 
TGCCTGGATG TGTCGGAGAA CAAGCTGGAG CAGTTGCCCA ACGAGGTCAG CGGCCTGGTG 
GCACTGACCG ACTTGCTGCT GTCGCAGAAC CTGTTGGAGT GCATTCCTGA TGGGATTGGT 
CAGCTGAAGC AGCTCTCCAT CCTCAAGGTG GACCAGAATC GACTGACAGA GGTGACAGAG 
TCCATTGGGG ACTGTGAAAA TCTGTCCGAG CTCATCCTGA CAGAGAACAT GCTGACGGCC 
TTGCCGAAAT CCCTTGGGAA GCTGGCCAAG CTGACCAACC TGAACGTGGA TCGGAACCGG 
CTGACATCCC TCCCGGCCGA GATCGGTGGC TGTGCCAACC TCAACGTGCT GTCCTTGCGG 
GACAATCGCC TGACCCTCCT GCCATCGGAG CTGGCCAACA CCACCGAGCT TCATGTTCTG 
GATGTGGCTG GGAACAGGCT GCAGAACTTG CCGTTTGCTC TGACAAACCT CAACCTGAAA 
GCCCTGTGGC TGGCGGAGAA TCAGTCCCAG CCCATGCTGA AATTCCAAAC AGAGGATGAT 
GAGAAGACAG GAGAGAAAGT TCTCACTTGT TACCTGCTTC CTCAGCAGCC CTCTCCCAGC 
CTGGAGAACC TTCTACAGAA CAGCGTGGAC GAGAGCTGGA CGGACACCAA TTTAAACAGA 
GTCAGTGTGA TTCAGTTCCT GGATGAACCC AAAGCAGATG ATGAAGAAGA GTCTGGAGCT 
GAGAGAAGGG GCTTGCAGCG CAGAGCAACC CCCCATCCCA GCGAGCTGAA GGTGATGAAG 
AAGGTGATTG AAGTTCGGCG CAGTGAGGCC CACGCTGCAA AGCCCAATGC AGACCTGAAT 
TCTCCTCACT CAGAAGAGAA GAGGCTGAGC GCCACGTCCA ACCGCAGCGA GGACTCCCAC 
CTTTCCAACA GCACTGCCTC TGCTGACTGT AGGGCGGAGG AGCAGCAGGA GGAGGCAGAG 
AGGAGTCGGC CAAACGGGCA AGTGCCTGAG ATGCAGGAGC TGGAGAAGGA GGCAAAGTCC 
AGAGTGGACG AGGAGCACAA GGAAGCTGCT GGCCATGAAG AGGAAGATGT GGAAGTGGAA 
TACAATGAGC CCACTGTACA CTTTGCTGAA GACACGCTAA TACGGAGTGA GGATGAAGAT 
GAAGATGAAG AGCGACCGTT CCCAGTGGAG AAGCAGCGGC TCATCCGCAA GGACACCCCT 
CACTATAAGA AACACTTTAA GATAACTAAA TTGCCCAAGC CAGAGGCGGT GGTGGCCCTG 
CTGCAGGGGC TGAACAGCGA GGGGGTCCCC AAGGAGGAAG AGGAGTTGGG AGGCTGCAAC 
AACAACACAG AGCCTGCAGA CCAGGAGGAG GTGTGGGATG AGGAGGACCG GAAGAATGGA 
GCTTTGTCTG CCCAGCCATC GGTGAAGGGG GTGTCGTTTG ATCAAGCCAA TAATCTGCTG 
ATTGAACCTG CTCGCATTGA GGAGGAAGAG TTGACTCTGA CTATCGTACG GCAGACGGGG 
GGGCTGGGCA TCAGCATCGC AGGGGGCAAG GGCTCCACCC CCTATAAGGG CGATGATGAG 
GGCATCTTTA TTTCTCGGGT GTCAGAAGAA GGTCCTGCGG CTCGTGCAGG GGTTCGTGTT 
GGGGACAAGC TCCTGGAGGT GAACGGTGTG TCCCTGCACT CCGCTGAGCA CCACGTGGCA 
GTGGAGGCTC TGCGTGGATC GGGAAGTTCC GTCTCCATGA CTGTTCTGCG GGAGCGGATG 
GTGGAGCCGG AGAATGCCAT CACTGTCACC CCGCTGCGCC CCGAGGATGA CTACAGCCCC 
CGTGAGAGGC GAGGTGGCCT GCGCTTCCCG GAGCGACCCG AGGGGACACC TCCCACCGAG 
AGATACTCCA CGTGCCTCAT GCGCAACGAG AAGGGACTGG GCTTCAGTAT CGCTGGTGGC 
AAAGGCTCCA CTCCTTACCG GGCTGGTGAC ATGGGGATCT TTATCTCCCG GATCGCAGAG 
GGTGGTGCAG CCCATCGGGA CGGGATCCTG CACGTAGGAG ATCGGGTCAT CTCGATCAAT 
GGGGTAGACA TGACAGAAGC CAGGCATGAT CAGGCAGTAG CGCTGCTTAC CGCGTCCTCC 
CCCACCATCG TGCTGCTGGT AGAGAGAGAG GGTGCGGAGC AGCCCGGCGA GGGAGACTGG 
CCCGGGGGGC CACGGGCACG CATGCACTCC CCACCGCCAC CCCCCAGCCA TGGGGAGAGC 
CCGCCCGAGG AGACGCCCGC ACCACAGAGG AACCATCTCT CCAAAGGCCT GGAGGATCAG 
TACCCCATTG AGGAAATTCA CCTGGTGAAA GCAGGTGGTC CCCTGGGGCT CAGCATCGTA 
GGGGGCAGTG ACCACTCAAG CCATCCGTTT GGGATTCATG AACCAGGTGT CTTCATTTCG 
AAGGTCATTC CCCGTGGGCT GGCATCCCGC AGTGGGCTGC GAGTAGGGGA CAGGATCCTG 
GAAGTCAATA GCATTGACCT GCGCCACGCT ACCCACCAGG AAGCGGTGAA CGCCCTGCTC 
TCCAACACCC AGGAGCTGAC CATGTTAGTG AGGCGAGACC CACCACCACC TGGTATGCAG 
GAAATATGCA TTGAGAAGGC TCCAGGAGAA AAGCTGGGCA TCAGCATCCG GGGTGGAGCG 
AAAGGCCATG CTGGAAATCC GTTTGATCCC ACTGACGAGG GCATCTTCAT CTCTAAGGTG 
AGCTCCTCGG GGGCGGCAGC CCGGGACGGC CGCCTGAAGG TGGGGATGCG GATCCTAGAG 
GTGAACCACC AGAGCCTGCT GGGGATGACG CACACGGAAG CGGTGCAGAT GCTGCGCGGG 
GTGGGCGACG CGCTCCTGGT GCTGGTGTGT GACGGCTTCG ACCCCAAGGC TGCAGCTGCC 
ATCGAGATGT CCCCGGGAAT TATTGCAAAC CCTTTCGCTG CTGGTATCGG ACGCAAGAAT 
AGCCTGGAAA GTATCTCTTC CATCGACCGG GATTTAAGCC CTGAGGAACT GGAGATCCTG 
CAGAAGGAGA TGGAAATGGT GAGAGAAGCA ACTCAGTGGG AGAGAGAAGA AATGGAGAAA 
GTGGAGCGGA TGCGTAGGGA GCGGGAGGCA GCCACCCGGC AGCTTGAGGA GGAGGCGGAG 
AACGTCACCA GCGAGCCCTT GAGACTGGAC TATCGGACCC TGGCTGCGCT GCCGAGCAGT 
GGTCTCCAGA GAGTCAACCG CACCCTCGTT CAGTATAATA AGCCCAGCTC CAGAATGGAG 
GGCGACTGTG CTCTGCTGTC TCTGCCGGTG GCACGGGACA GCCGGGATTC ATGCCCTGAC 
ACGGCTGCTG GCCCCACCAT CGGAAGCGTG GCCAGACCTC CCCGGGACTC CGCAACACCC 
GACCACTGCA CCAGCGGGGC AGAAACGCCC CGGCCTGACC AGCAGCCTCC CACAGGTGGC 
CCCTTGTCCC AGGACCAGCC GCCAAAGACG CCAGAGCAAG AGTGCATGGT GGACTCCCAG 
CCCATCCTCT TCAGTGAGAA CCCCTTTGTG GTGGCCAACC GCAGGGGGAA GGCGGCCGGC 
CGGGCCTGCC TGGGGGGGCC TCCCCTGGGC TATGGCAGGG GGGGAGTGCT GAAGACCAAC 
CTTTACTCCA AGGCTCCTGC AGGCGAGTCT GGGATGGGCA ATTCCAGGCG TGAAACCCCC 
TATTCTCCTG GTAACCAGCA GCGGCCTGCA ACCCCTCGGC CCGCTCACCT GCCGGGCAGG 
GAGACCCGCT TTGCTTCCAT TAACTTCATT ACATCGCAGG ATGACAGTAA ATCGACCAAG 
CCAGGCGTCA TTCAGCCTCT TTCCCGAGTG CGACAAAATG CTGCCCCGCA CAATTCGGAG 
GAGGGGGACA GCCCCAATCC CTTCCAGCAG CCCGAGCCGT GCTTCAGTGT CCAGAACTCT 
CCAAAGCCAC CCTCACCACC CTCTCCTGAT GAGGTCCCCA TCAATGTCAA GCAAGCGTAC 
AAGACCTTCG CTGCAGTGCC CTTGTCACAC CCCCTGCTTG GGACACAGGA ATTGCCTGGT 
CAGAGCGGCA CAGAGAATCC CAGAAGTGCC CCGGAGGTGG CTGCGCACAG CCCTGGGGGA 
CTGCACAGCC CTGAGCAGAA GTCCTTCCGT GAGAGGCAGA AGTATTTCGA GATTGAGGTG 
AAGCAGCAGC AGATGGACAA GCCCCCCAAG AGAGTCTCCT TGGTGGGAGA GGACGACCTG 
AAGAAGATGA AAGAGGAGGA AGCTCGAAAA CTGCAGCAGA AACGTACCCT TCTGCTGGAG 
GAAGAGGCAG AAGAGGAGGA GATGGCGAAG CAGGCGCCTG AGGTGAGCGT GCAGTCCAGT 
GTCATCATTG AAGGAGTCGA GTACAAGATT GAGAGACTCA ACGGGAGGAG CAGCCAGTCT 
CCGGCCACTC GGCCCTCCGA GGTCAACGAG AACAGCAGCT CGCAGAGGAA TTCACTGGAA 
GAGGGGATCA AGGCTGAGCA GAGAACCAAC TCCATGTCTG GGCTGAGTCC CTTGTACCTT 
GGAGCAGGGG ACGCAGTGGC ACCCGTGCGG ACGGCCAAAG CTGAACGGCG ACACCAGGAG 
CGGTTGCGAA TGCAGAGTCC TGAGCTGCTG AGTGGCCAAG AGAAGGAGCT TTCTCCTGCA 
GAACGTCGTG CTCTGGAGGC GGAGAAACGA GCAATGTGGA GGGCAGCACG ATCCTCAGCT 
CTTGAAGACA GTATTAAACA GTACGAGCAG GACCTGGCCA GGAGGCTGTA CCAGTCCCGA 
CAGCGGGCAC CTGCTCCCGG GGCCGGGCCA GCTCCGATGC CTAGTCCCAA GCAGAGTCAC 
GCCTCGGCAC CCCCTGGGTC AGGAAGCCAT TCCAGGATGA AATCGCTTGA ACAGGATGCT 
CTGAAAGCCC AGATGGTCAT TGCCAAGTCC AAGGAGGGGA AGAAGCGCAG CCCGCTGGAG 
CAGCTGGCAG AGTCCCCCTC GCCAGTTCCT ACACCGTCAC CGACACCACT GGAAGATTGC 
AGTCCCAGGA ACGTCACCTC CCCAGGAAGG CTGTCCATGT CTGAAAAGAA GTTTGACTAC 
CGGGAATTTG CTGCTATTCC TTCCTCCAAA CCCGTTTACG AAATTCAGTC GCCTGATGCT 
GCTGATGACC TCAGATACAT CGAGGACAGG AATAACTCAG TTCCCCAGGA GCAGGATGGG 
CAGCCCGAGG AAGAGGACAC ATTAGGAACT CACGACTCAT CCACACCCAC TCCATCAGCA 
CTTGAAGAAA TGGCCCTGTA CAGCAGCAAA CGCAAGCTCC GACACAGCCG GCGCAGCCTG 
GAGGCTGCCG TCCCTACGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014801200.1
CDS	6..5966
Translation	MPPLWRCNRHVEALDRRHCSLQAVPEEIYRYSRSLEELLLDANQLRELPKPFFRLLNLRKLGLSDNEIQRLPPEVANFMQLVELDISRNDIPEIPESIKFCKSLEIADFSGNPLSRLPEGFTQLRSLGHLALNDVSLQSLPSDIGNLANLVTLELRENLLKTLPTSLSFLVKLEQLDLGGNDLEVLPDTLGALPNLRELWLDRNQLSALPPELGNLRRLVCLDVSENKLEQLPNEVSGLVALTDLLLSQNLLECIPDGIGQLKQLSILKVDQNRLTEVTESIGDCENLSELILTENMLTALPKSLGKLAKLTNLNVDRNRLTSLPAEIGGCANLNVLSLRDNRLTLLPSELANTTELHVLDVAGNRLQNLPFALTNLNLKALWLAENQSQPMLKFQTEDDEKTGEKVLTCYLLPQQPSPSLENLLQNSVDESWTDTNLNRVSVIQFLDEPKADDEEESGAERRGLQRRATPHPSELKVMKKVIEVRRSEAHAAKPNADLNSPHSEEKRLSATSNRSEDSHLSNSTASADCRAEEQQEEAERSRPNGQVPEMQELEKEAKSRVDEEHKEAAGHEEEDVEVEYNEPTVHFAEDTLIRSEDEDEDEERPFPVEKQRLIRKDTPHYKKHFKITKLPKPEAVVALLQGLNSEGVPKEEEELGGCNNNTEPADQEEVWDEEDRKNGALSAQPSVKGVSFDQANNLLIEPARIEEEELTLTIVRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVRVGDKLLEVNGVSLHSAEHHVAVEALRGSGSSVSMTVLRERMVEPENAITVTPLRPEDDYSPRERRGGLRFPERPEGTPPTERYSTCLMRNEKGLGFSIAGGKGSTPYRAGDMGIFISRIAEGGAAHRDGILHVGDRVISINGVDMTEARHDQAVALLTASSPTIVLLVEREGAEQPGEGDWPGGPRARMHSPPPPPSHGESPPEETPAPQRNHLSKGLEDQYPIEEIHLVKAGGPLGLSIVGGSDHSSHPFGIHEPGVFISKVIPRGLASRSGLRVGDRILEVNSIDLRHATHQEAVNALLSNTQELTMLVRRDPPPPGMQEICIEKAPGEKLGISIRGGAKGHAGNPFDPTDEGIFISKVSSSGAAARDGRLKVGMRILEVNHQSLLGMTHTEAVQMLRGVGDALLVLVCDGFDPKAAAAIEMSPGIIANPFAAGIGRKNSLESISSIDRDLSPEELEILQKEMEMVREATQWEREEMEKVERMRREREAATRQLEEEAENVTSEPLRLDYRTLAALPSSGLQRVNRTLVQYNKPSSRMEGDCALLSLPVARDSRDSCPDTAAGPTIGSVARPPRDSATPDHCTSGAETPRPDQQPPTGGPLSQDQPPKTPEQECMVDSQPILFSENPFVVANRRGKAAGRACLGGPPLGYGRGGVLKTNLYSKAPAGESGMGNSRRETPYSPGNQQRPATPRPAHLPGRETRFASINFITSQDDSKSTKPGVIQPLSRVRQNAAPHNSEEGDSPNPFQQPEPCFSVQNSPKPPSPPSPDEVPINVKQAYKTFAAVPLSHPLLGTQELPGQSGTENPRSAPEVAAHSPGGLHSPEQKSFRERQKYFEIEVKQQQMDKPPKRVSLVGEDDLKKMKEEEARKLQQKRTLLLEEEAEEEEMAKQAPEVSVQSSVIIEGVEYKIERLNGRSSQSPATRPSEVNENSSSQRNSLEEGIKAEQRTNSMSGLSPLYLGAGDAVAPVRTAKAERRHQERLRMQSPELLSGQEKELSPAERRALEAEKRAMWRAARSSALEDSIKQYEQDLARRLYQSRQRAPAPGAGPAPMPSPKQSHASAPPGSGSHSRMKSLEQDALKAQMVIAKSKEGKKRSPLEQLAESPSPVPTPSPTPLEDCSPRNVTSPGRLSMSEKKFDYREFAAIPSSKPVYEIQSPDAADDLRYIEDRNNSVPQEQDGQPEEEDTLGTHDSSTPTPSALEEMALYSSKRKLRHSRRSLEAAVPT

Target ORF information:

RefSeq Version	XM_014945714.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax scribbled planar cell polarity protein (SCRIB), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014945714.1

ORF Insert Sequence:

ATGCCGCCGC TCTGGCGCTG CAACCGGCAC GTGGAGGCGC TGGACCGGCG GCACTGCTCG 
CTGCAGGCCG TGCCCGAGGA GATCTACCGC TACAGCCGCT CGCTGGAGGA GCTGCTGCTG 
GACGCCAACC AGCTGCGGGA GCTGCCCAAG CCTTTCTTCA GGCTTCTGAA CCTGCGCAAG 
CTGGGCCTGA GTGACAATGA GATCCAAAGA CTTCCCCCTG AGGTGGCCAA CTTCATGCAG 
CTGGTGGAAC TGGACATCTC CCGTAACGAC ATTCCAGAAA TTCCTGAAAG CATCAAATTC 
TGCAAATCCT TGGAAATCGC AGACTTCAGT GGGAATCCTC TCTCCAGGCT TCCCGAGGGC 
TTCACGCAGC TTCGGAGCCT GGGCCATTTG GCCCTGAATG ATGTGTCCCT GCAGAGCCTC 
CCCAGTGACA TTGGGAATTT GGCAAACCTG GTGACTCTGG AGCTACGCGA GAACCTGCTA 
AAGACTCTCC CCACCTCGCT GTCCTTCCTT GTCAAGTTAG AGCAGCTGGA TCTCGGTGGC 
AATGACCTGG AGGTTCTGCC AGACACTCTG GGAGCGCTCC CCAACCTGCG TGAGCTCTGG 
TTAGACCGGA ACCAGCTCTC AGCCCTGCCT CCCGAGCTGG GCAACCTCCG CCGCCTGGTC 
TGCCTGGATG TGTCGGAGAA CAAGCTGGAG CAGTTGCCCA ACGAGGTCAG CGGCCTGGTG 
GCACTGACCG ACTTGCTGCT GTCGCAGAAC CTGTTGGAGT GCATTCCTGA TGGGATTGGT 
CAGCTGAAGC AGCTCTCCAT CCTCAAGGTG GACCAGAATC GACTGACAGA GGTGACAGAG 
TCCATTGGGG ACTGTGAAAA TCTGTCCGAG CTCATCCTGA CAGAGAACAT GCTGACGGCC 
TTGCCGAAAT CCCTTGGGAA GCTGGCCAAG CTGACCAACC TGAACGTGGA TCGGAACCGG 
CTGACATCCC TCCCGGCCGA GATCGGTGGC TGTGCCAACC TCAACGTGCT GTCCTTGCGG 
GACAATCGCC TGACCCTCCT GCCATCGGAG CTGGCCAACA CCACCGAGCT TCATGTTCTG 
GATGTGGCTG GGAACAGGCT GCAGAACTTG CCGTTTGCTC TGACAAACCT CAACCTGAAA 
GCCCTGTGGC TGGCGGAGAA TCAGTCCCAG CCCATGCTGA AATTCCAAAC AGAGGATGAT 
GAGAAGACAG GAGAGAAAGT TCTCACTTGT TACCTGCTTC CTCAGCAGCC CTCTCCCAGC 
CTGGAGAACC TTCTACAGAA CAGCGTGGAC GAGAGCTGGA CGGACACCAA TTTAAACAGA 
GTCAGTGTGA TTCAGTTCCT GGATGAACCC AAAGCAGATG ATGAAGAAGA GTCTGGAGCT 
GAGAGAAGGG GCTTGCAGCG CAGAGCAACC CCCCATCCCA GCGAGCTGAA GGTGATGAAG 
AAGGTGATTG AAGTTCGGCG CAGTGAGGCC CACGCTGCAA AGCCCAATGC AGACCTGAAT 
TCTCCTCACT CAGAAGAGAA GAGGCTGAGC GCCACGTCCA ACCGCAGCGA GGACTCCCAC 
CTTTCCAACA GCACTGCCTC TGCTGACTGT AGGGCGGAGG AGCAGCAGGA GGAGGCAGAG 
AGGAGTCGGC CAAACGGGCA AGTGCCTGAG ATGCAGGAGC TGGAGAAGGA GGCAAAGTCC 
AGAGTGGACG AGGAGCACAA GGAAGCTGCT GGCCATGAAG AGGAAGATGT GGAAGTGGAA 
TACAATGAGC CCACTGTACA CTTTGCTGAA GACACGCTAA TACGGAGTGA GGATGAAGAT 
GAAGATGAAG AGCGACCGTT CCCAGTGGAG AAGCAGCGGC TCATCCGCAA GGACACCCCT 
CACTATAAGA AACACTTTAA GATAACTAAA TTGCCCAAGC CAGAGGCGGT GGTGGCCCTG 
CTGCAGGGGC TGAACAGCGA GGGGGTCCCC AAGGAGGAAG AGGAGTTGGG AGGCTGCAAC 
AACAACACAG AGCCTGCAGA CCAGGAGGAG GTGTGGGATG AGGAGGACCG GAAGAATGGA 
GCTTTGTCTG CCCAGCCATC GGTGAAGGGG GTGTCGTTTG ATCAAGCCAA TAATCTGCTG 
ATTGAACCTG CTCGCATTGA GGAGGAAGAG TTGACTCTGA CTATCGTACG GCAGACGGGG 
GGGCTGGGCA TCAGCATCGC AGGGGGCAAG GGCTCCACCC CCTATAAGGG CGATGATGAG 
GGCATCTTTA TTTCTCGGGT GTCAGAAGAA GGTCCTGCGG CTCGTGCAGG GGTTCGTGTT 
GGGGACAAGC TCCTGGAGGT GAACGGTGTG TCCCTGCACT CCGCTGAGCA CCACGTGGCA 
GTGGAGGCTC TGCGTGGATC GGGAAGTTCC GTCTCCATGA CTGTTCTGCG GGAGCGGATG 
GTGGAGCCGG AGAATGCCAT CACTGTCACC CCGCTGCGCC CCGAGGATGA CTACAGCCCC 
CGTGAGAGGC GAGGTGGCCT GCGCTTCCCG GAGCGACCCG AGGGGACACC TCCCACCGAG 
AGATACTCCA CGTGCCTCAT GCGCAACGAG AAGGGACTGG GCTTCAGTAT CGCTGGTGGC 
AAAGGCTCCA CTCCTTACCG GGCTGGTGAC ATGGGGATCT TTATCTCCCG GATCGCAGAG 
GGTGGTGCAG CCCATCGGGA CGGGATCCTG CACGTAGGAG ATCGGGTCAT CTCGATCAAT 
GGGGTAGACA TGACAGAAGC CAGGCATGAT CAGGCAGTAG CGCTGCTTAC CGCGTCCTCC 
CCCACCATCG TGCTGCTGGT AGAGAGAGAG GGTGCGGAGC AGCCCGGCGA GGGAGACTGG 
CCCGGGGGGC CACGGGCACG CATGCACTCC CCACCGCCAC CCCCCAGCCA TGGGGAGAGC 
CCGCCCGAGG AGACGCCCGC ACCACAGAGG AACCATCTCT CCAAAGGCCT GGAGGATCAG 
TACCCCATTG AGGAAATTCA CCTGGTGAAA GCAGGTGGTC CCCTGGGGCT CAGCATCGTA 
GGGGGCAGTG ACCACTCAAG CCATCCGTTT GGGATTCATG AACCAGGTGT CTTCATTTCG 
AAGGTCATTC CCCGTGGGCT GGCATCCCGC AGTGGGCTGC GAGTAGGGGA CAGGATCCTG 
GAAGTCAATA GCATTGACCT GCGCCACGCT ACCCACCAGG AAGCGGTGAA CGCCCTGCTC 
TCCAACACCC AGGAGCTGAC CATGTTAGTG AGGCGAGACC CACCACCACC TGGTATGCAG 
GAAATATGCA TTGAGAAGGC TCCAGGAGAA AAGCTGGGCA TCAGCATCCG GGGTGGAGCG 
AAAGGCCATG CTGGAAATCC GTTTGATCCC ACTGACGAGG GCATCTTCAT CTCTAAGGTG 
AGCTCCTCGG GGGCGGCAGC CCGGGACGGC CGCCTGAAGG TGGGGATGCG GATCCTAGAG 
GTGAACCACC AGAGCCTGCT GGGGATGACG CACACGGAAG CGGTGCAGAT GCTGCGCGGG 
GTGGGCGACG CGCTCCTGGT GCTGGTGTGT GACGGCTTCG ACCCCAAGGC TGCAGCTGCC 
ATCGAGATGT CCCCGGGAAT TATTGCAAAC CCTTTCGCTG CTGGTATCGG ACGCAAGAAT 
AGCCTGGAAA GTATCTCTTC CATCGACCGG GATTTAAGCC CTGAGGAACT GGAGATCCTG 
CAGAAGGAGA TGGAAATGGT GAGAGAAGCA ACTCAGTGGG AGAGAGAAGA AATGGAGAAA 
GTGGAGCGGA TGCGTAGGGA GCGGGAGGCA GCCACCCGGC AGCTTGAGGA GGAGGCGGAG 
AACGTCACCA GCGAGCCCTT GAGACTGGAC TATCGGACCC TGGCTGCGCT GCCGAGCAGT 
GGTCTCCAGA GAGTCAACCG CACCCTCGTT CAGTATAATA AGCCCAGCTC CAGAATGGAG 
GGCGACTGTG CTCTGCTGTC TCTGCCGGTG GCACGGGACA GCCGGGATTC ATGCCCTGAC 
ACGGCTGCTG GCCCCACCAT CGGAAGCGTG GCCAGACCTC CCCGGGACTC CGCAACACCC 
GACCACTGCA CCAGCGGGGC AGAAACGCCC CGGCCTGACC AGCAGCCTCC CACAGGTGGC 
CCCTTGTCCC AGGACCAGCC GCCAAAGACG CCAGAGCAAG AGTGCATGGT GGACTCCCAG 
CCCATCCTCT TCAGTGAGAA CCCCTTTGTG GTGGCCAACC GCAGGGGGAA GGCGGCCGGC 
CGGGCCTGCC TGGGGGGGCC TCCCCTGGGC TATGGCAGGG GGGGAGTGCT GAAGACCAAC 
CTTTACTCCA AGGCTCCTGC AGGCGAGTCT GGGATGGGCA ATTCCAGGCG TGAAACCCCC 
TATTCTCCTG GTAACCAGCA GCGGCCTGCA ACCCCTCGGC CCGCTCACCT GCCGGGCAGG 
GAGACCCGCT TTGCTTCCAT TAACTTCATT ACATCGCAGG ATGACAGTAA ATCGACCAAG 
CCAGGCGTCA TTCAGCCTCT TTCCCGAGTG CGACAAAATG CTGCCCCGCA CAATTCGGAG 
GAGGGGGACA GCCCCAATCC CTTCCAGCAG CCCGAGCCGT GCTTCAGTGT CCAGAACTCT 
CCAAAGCCAC CCTCACCACC CTCTCCTGAT GAGGTCCCCA TCAATGTCAA GCAAGCGTAC 
AAGACCTTCG CTGCAGTGCC CTTGTCACAC CCCCTGCTTG GGACACAGGA ATTGCCTGGT 
CAGAGCGGCA CAGAGAATCC CAGAAGTGCC CCGGAGGTGG CTGCGCACAG CCCTGGGGGA 
CTGCACAGCC CTGAGCAGAA GTCCTTCCGT GAGAGGCAGA AGTATTTCGA GATTGAGGTG 
AAGCAGCAGC AGATGGACAA GCCCCCCAAG AGAGTCTCCT TGGTGGGAGA GGACGACCTG 
AAGAAGATGA AAGAGGAGGA AGCTCGAAAA CTGCAGCAGA AACGTACCCT TCTGCTGGAG 
GAAGAGGCAG AAGAGGAGGA GATGGCGAAG CAGGCGCCTG AGGTGAGCGT GCAGTCCAGT 
GTCATCATTG AAGGAGTCGA GTACAAGATT GAGAGACTCA ACGGGAGGAG CAGCCAGTCT 
CCGGCCACTC GGCCCTCCGA GGTCAACGAG AACAGCAGCT CGCAGAGGAA TTCACTGGAA 
GAGGGGATCA AGGCTGAGCA GAGAACCAAC TCCATGTCTG GGCTGAGTCC CTTGTACCTT 
GGAGCAGGGG ACGCAGTGGC ACCCGTGCGG ACGGCCAAAG CTGAACGGCG ACACCAGGAG 
CGGTTGCGAA TGCAGAGTCC TGAGCTGCTG AGTGGCCAAG AGAAGGAGCT TTCTCCTGCA 
GAACGTCGTG CTCTGGAGGC GGAGAAACGA GCAATGTGGA GGGCAGCACG ATCCTCAGCT 
CTTGAAGACA GTATTAAACA GTACGAGCAG GACCTGGCCA GGAGGCTGTA CCAGTCCCGA 
CAGCGGGCAC CTGCTCCCGG GGCCGGGCCA GCTCCGATGC CTAGTCCCAA GCAGAGTCAC 
GCCTCGGCAC CCCCTGGGTC AGGAAGCCAT TCCAGGATGA AATCGCTTGA ACAGGATGCT 
CTGAAAGCCC AGATGGTCAT TGCCAAGTCC AAGGAGGGGA AGAAGCGCAG CCCGCTGGAG 
CAGCTGGCAG AGTCCCCCTC GCCAGTTCCT ACACCGTCAC CGACACCACT GGAAGATTGC 
AGTCCCAGGA ACGTCACCTC CCCAGGAAGG CTGTCCATGT CTGAAAAGAA GTTTGACTAC 
CGGGAATTTG CTGCTATTCC TTCCTCCAAA CCCGTTTACG AAATTCAGTC GCCTGATGCT 
GCTGATGACC TCAGATACAT CGAGGACAGG AATAACTCAG TTCCCCAGGA GCAGGATGGG 
CAGCCCGAGG AAGAGGACAC ATTAGGAACT CACGACTCAT CCACACCCAC TCCATCAGCA 
CTTGAAGAAA TGGCCCTGTA CAGCAGCAAA CGCAAGCTCC GACACAGCCG GCGCAGCCTG 
GAGGCTGCCG TCCCTACGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCRIB ( XM_014945715.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014801201.1 Calidris pugnax scribbled planar cell polarity protein (SCRIB), transcript variant X2, mRNA.

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CloneID	OCa67748
Clone ID Related Accession (Same CDS sequence)	XM_014945715.1
Accession Version	XM_014945715.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5910bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	protein scribble homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090871.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCCGC TCTGGCGCTG CAACCGGCAC GTGGAGGCGC TGGACCGGCG GCACTGCTCG 
CTGCAGGCCG TGCCCGAGGA GATCTACCGC TACAGCCGCT CGCTGGAGGA GCTGCTGCTG 
GACGCCAACC AGCTGCGGGA GCTGCCCAAG CCTTTCTTCA GGCTTCTGAA CCTGCGCAAG 
CTGGGCCTGA GTGACAATGA GATCCAAAGA CTTCCCCCTG AGGTGGCCAA CTTCATGCAG 
CTGGTGGAAC TGGACATCTC CCGTAACGAC ATTCCAGAAA TTCCTGAAAG CATCAAATTC 
TGCAAATCCT TGGAAATCGC AGACTTCAGT GGGAATCCTC TCTCCAGGCT TCCCGAGGGC 
TTCACGCAGC TTCGGAGCCT GGGCCATTTG GCCCTGAATG ATGTGTCCCT GCAGAGCCTC 
CCCAGTGACA TTGGGAATTT GGCAAACCTG GTGACTCTGG AGCTACGCGA GAACCTGCTA 
AAGACTCTCC CCACCTCGCT GTCCTTCCTT GTCAAGTTAG AGCAGCTGGA TCTCGGTGGC 
AATGACCTGG AGGTTCTGCC AGACACTCTG GGAGCGCTCC CCAACCTGCG TGAGCTCTGG 
TTAGACCGGA ACCAGCTCTC AGCCCTGCCT CCCGAGCTGG GCAACCTCCG CCGCCTGGTC 
TGCCTGGATG TGTCGGAGAA CAAGCTGGAG CAGTTGCCCA ACGAGGTCAG CGGCCTGGTG 
GCACTGACCG ACTTGCTGCT GTCGCAGAAC CTGTTGGAGT GCATTCCTGA TGGGATTGGT 
CAGCTGAAGC AGCTCTCCAT CCTCAAGGTG GACCAGAATC GACTGACAGA GGTGACAGAG 
TCCATTGGGG ACTGTGAAAA TCTGTCCGAG CTCATCCTGA CAGAGAACAT GCTGACGGCC 
TTGCCGAAAT CCCTTGGGAA GCTGGCCAAG CTGACCAACC TGAACGTGGA TCGGAACCGG 
CTGACATCCC TCCCGGCCGA GATCGGTGGC TGTGCCAACC TCAACGTGCT GTCCTTGCGG 
GACAATCGCC TGACCCTCCT GCCATCGGAG CTGGCCAACA CCACCGAGCT TCATGTTCTG 
GATGTGGCTG GGAACAGGCT GCAGAACTTG CCGTTTGCTC TGACAAACCT CAACCTGAAA 
GCCCTGTGGC TGGCGGAGAA TCAGTCCCAG CCCATGCTGA AATTCCAAAC AGAGGATGAT 
GAGAAGACAG GAGAGAAAGT TCTCACTTGT TACCTGCTTC CTCAGCAGCC CTCTCCCAGC 
CTGGAGAACC TTCTACAGAA CAGCGTGGAC GAGAGCTGGA CGGACACCAA TTTAAACAGA 
GTCAGTGTGA TTCAGTTCCT GGATGAACCC AAAGCAGATG ATGAAGAAGA GTCTGGAGCT 
GAGAGAAGGG GCTTGCAGCG CAGAGCAACC CCCCATCCCA GCGAGCTGAA GGTGATGAAG 
AAGGTGATTG AAGTTCGGCG CAGTGAGGCC CACGCTGCAA AGCCCAATGC AGACCTGAAT 
TCTCCTCACT CAGAAGAGAA GAGGCTGAGC GCCACGTCCA ACCGCAGCGA GGACTCCCAC 
CTTTCCAACA GCACTGCCTC TGCTGACTGT AGGGCGGAGG AGCAGCAGGA GGAGGCAGAG 
AGGAGTCGGC CAAACGGGCA AGTGCCTGAG ATGCAGGAGC TGGAGAAGGA GGCAAAGTCC 
AGAGTGGACG AGGAGCACAA GGAAGCTGCT GGCCATGAAG AGGAAGATGT GGAAGTGGAA 
TACAATGAGC CCACTGTACA CTTTGCTGAA GACACGCTAA TACGGAGTGA GGATGAAGAT 
GAAGATGAAG AGCGACCGTT CCCAGTGGAG AAGCAGCGGC TCATCCGCAA GGACACCCCT 
CACTATAAGA AACACTTTAA GATAACTAAA TTGCCCAAGC CAGAGGCGGT GGTGGCCCTG 
CTGCAGGGGC TGAACAGCGA GGGGGTCCCC AAGGAGGAAG AGGAGTTGGG AGGCTGCAAC 
AACAACACAG AGCCTGCAGA CCAGGAGGAG GTGTGGGATG AGGAGGACCG GAAGAATGGA 
GCTTTGTCTG CCCAGCCATC GGTGAAGGGG GTGTCGTTTG ATCAAGCCAA TAATCTGCTG 
ATTGAACCTG CTCGCATTGA GGAGGAAGAG TTGACTCTGA CTATCGTACG GCAGACGGGG 
GGGCTGGGCA TCAGCATCGC AGGGGGCAAG GGCTCCACCC CCTATAAGGG CGATGATGAG 
GGCATCTTTA TTTCTCGGGT GTCAGAAGAA GGTCCTGCGG CTCGTGCAGG GGTTCGTGTT 
GGGGACAAGC TCCTGGAGGT GAACGGTGTG TCCCTGCACT CCGCTGAGCA CCACGTGGCA 
GTGGAGGCTC TGCGTGGATC GGGAAGTTCC GTCTCCATGA CTGTTCTGCG GGAGCGGATG 
GTGGAGCCGG AGAATGCCAT CACTGTCACC CCGCTGCGCC CCGAGGATGA CTACAGCCCC 
CGTGAGAGGC GAGGTGGCCT GCGCTTCCCG GAGCGACCCG AGGGGACACC TCCCACCGAG 
AGATACTCCA CGTGCCTCAT GCGCAACGAG AAGGGACTGG GCTTCAGTAT CGCTGGTGGC 
AAAGGCTCCA CTCCTTACCG GGCTGGTGAC ATGGGGATCT TTATCTCCCG GATCGCAGAG 
GGTGGTGCAG CCCATCGGGA CGGGATCCTG CACGTAGGAG ATCGGGTCAT CTCGATCAAT 
GGGGTAGACA TGACAGAAGC CAGGCATGAT CAGGCAGTAG CGCTGCTTAC CGCGTCCTCC 
CCCACCATCG TGCTGCTGGT AGAGAGAGAG GGTGCGGAGC AGCCCGGCGA GGGAGACTGG 
CCCGGGGGGC CACGGGCACG CATGCACTCC CCACCGCCAC CCCCCAGCCA TGGGGAGAGC 
CCGCCCGAGG AGACGCCCGC ACCACAGAGG AACCATCTCT CCAAAGGCCT GGAGGATCAG 
TACCCCATTG AGGAAATTCA CCTGGTGAAA GCAGGTGGTC CCCTGGGGCT CAGCATCGTA 
GGGGGCAGTG ACCACTCAAG CCATCCGTTT GGGATTCATG AACCAGGTGT CTTCATTTCG 
AAGGTCATTC CCCGTGGGCT GGCATCCCGC AGTGGGCTGC GAGTAGGGGA CAGGATCCTG 
GAAGTCAATA GCATTGACCT GCGCCACGCT ACCCACCAGG AAGCGGTGAA CGCCCTGCTC 
TCCAACACCC AGGAGCTGAC CATGTTAGTG AGGCGAGACC CACCACCACC TGGTATGCAG 
GAAATATGCA TTGAGAAGGC TCCAGGAGAA AAGCTGGGCA TCAGCATCCG GGGTGGAGCG 
AAAGGCCATG CTGGAAATCC GTTTGATCCC ACTGACGAGG GCATCTTCAT CTCTAAGGTG 
AGCTCCTCGG GGGCGGCAGC CCGGGACGGC CGCCTGAAGG TGGGGATGCG GATCCTAGAG 
GTGAACCACC AGAGCCTGCT GGGGATGACG CACACGGAAG CGGTGCAGAT GCTGCGCGGG 
GTGGGCGACG CGCTCCTGGT GCTGGTGTGT GACGGCTTCG ACCCCAAGGC TGCAGCTGCC 
ATCGAGATGT CCCCGGGAAT TATTGCAAAC CCTTTCGCTG CTGGTATCGG ACGCAAGAAT 
AGCCTGGAAA GTATCTCTTC CATCGACCGG GATTTAAGCC CTGAGGAACT GGAGATCCTG 
CAGAAGGAGA TGGAAATGGT GAGAGAAGCA ACTCAGTGGG AGAGAGAAGA AATGGAGAAA 
GTGGAGCGGA TGCGTAGGGA GCGGGAGGCA GCCACCCGGC AGCTTGAGGA GGAGGCGGAG 
AACGTCACCA GCGAGCCCTT GAGACTGGAC TATCGGACCC TGGCTGCGCT GCCGAGCAGT 
GGTCTCCAGA GAGTCAACCG CACCCTCGTT CAGTATAATA AGCCCAGCTC CAGAATGGAG 
GGCGACTGTG CTCTGCTGTC TCTGCCGGTG GCACGGGACA GCCGGGATTC ATGCCCTGAC 
ACGGCTGCTG GCCCCACCAT CGGAAGCGTG GCCAGACCTC CCCGGGACTC CGCAACACCC 
GACCACTGCA CCAGCGGGGC AGAAACGCCC CGGCCTGACC AGCAGCCTCC CACAGGTGGC 
CCCTTGTCCC AGGACCAGCC GCCAAAGACG CCAGAGCAAG AGTGCATGGT GGACTCCCAG 
CCCATCCTCT TCAGTGAGAA CCCCTTTGTG GTGGCCAACC GCAGGGGGAA GGCGGCCGGC 
CGGGCCTGCC TGGGGGGGCC TCCCCTGGGC TATGGCAGGG GGGGAGTGCT GAAGACCAAC 
CTTTACTCCA AGGCTCCTGC AGGCGAGTCT GGGATGGGCA ATTCCAGGCG TGAAACCCCC 
TATTCTCCTG GTAACCAGCA GGCTTCCATT AACTTCATTA CATCGCAGGA TGACAGTAAA 
TCGACCAAGC CAGGCGTCAT TCAGCCTCTT TCCCGAGTGC GACAAAATGC TGCCCCGCAC 
AATTCGGAGG AGGGGGACAG CCCCAATCCC TTCCAGCAGC CCGAGCCGTG CTTCAGTGTC 
CAGAACTCTC CAAAGCCACC CTCACCACCC TCTCCTGATG AGGTCCCCAT CAATGTCAAG 
CAAGCGTACA AGACCTTCGC TGCAGTGCCC TTGTCACACC CCCTGCTTGG GACACAGGAA 
TTGCCTGGTC AGAGCGGCAC AGAGAATCCC AGAAGTGCCC CGGAGGTGGC TGCGCACAGC 
CCTGGGGGAC TGCACAGCCC TGAGCAGAAG TCCTTCCGTG AGAGGCAGAA GTATTTCGAG 
ATTGAGGTGA AGCAGCAGCA GATGGACAAG CCCCCCAAGA GAGTCTCCTT GGTGGGAGAG 
GACGACCTGA AGAAGATGAA AGAGGAGGAA GCTCGAAAAC TGCAGCAGAA ACGTACCCTT 
CTGCTGGAGG AAGAGGCAGA AGAGGAGGAG ATGGCGAAGC AGGCGCCTGA GGTGAGCGTG 
CAGTCCAGTG TCATCATTGA AGGAGTCGAG TACAAGATTG AGAGACTCAA CGGGAGGAGC 
AGCCAGTCTC CGGCCACTCG GCCCTCCGAG GTCAACGAGA ACAGCAGCTC GCAGAGGAAT 
TCACTGGAAG AGGGGATCAA GGCTGAGCAG AGAACCAACT CCATGTCTGG GCTGAGTCCC 
TTGTACCTTG GAGCAGGGGA CGCAGTGGCA CCCGTGCGGA CGGCCAAAGC TGAACGGCGA 
CACCAGGAGC GGTTGCGAAT GCAGAGTCCT GAGCTGCTGA GTGGCCAAGA GAAGGAGCTT 
TCTCCTGCAG AACGTCGTGC TCTGGAGGCG GAGAAACGAG CAATGTGGAG GGCAGCACGA 
TCCTCAGCTC TTGAAGACAG TATTAAACAG TACGAGCAGG ACCTGGCCAG GAGGCTGTAC 
CAGTCCCGAC AGCGGGCACC TGCTCCCGGG GCCGGGCCAG CTCCGATGCC TAGTCCCAAG 
CAGAGTCACG CCTCGGCACC CCCTGGGTCA GGAAGCCATT CCAGGATGAA ATCGCTTGAA 
CAGGATGCTC TGAAAGCCCA GATGGTCATT GCCAAGTCCA AGGAGGGGAA GAAGCGCAGC 
CCGCTGGAGC AGCTGGCAGA GTCCCCCTCG CCAGTTCCTA CACCGTCACC GACACCACTG 
GAAGATTGCA GTCCCAGGAA CGTCACCTCC CCAGGAAGGC TGTCCATGTC TGAAAAGAAG 
TTTGACTACC GGGAATTTGC TGCTATTCCT TCCTCCAAAC CCGTTTACGA AATTCAGTCG 
CCTGATGCTG CTGATGACCT CAGATACATC GAGGACAGGA ATAACTCAGT TCCCCAGGAG 
CAGGATGGGC AGCCCGAGGA AGAGGACACA TTAGGAACTC ACGACTCATC CACACCCACT 
CCATCAGCAC TTGAAGAAAT GGCCCTGTAC AGCAGCAAAC GCAAGCTCCG ACACAGCCGG 
CGCAGCCTGG AGGCTGCCGT CCCTACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014801201.1
CDS	6..5915
Translation	MPPLWRCNRHVEALDRRHCSLQAVPEEIYRYSRSLEELLLDANQLRELPKPFFRLLNLRKLGLSDNEIQRLPPEVANFMQLVELDISRNDIPEIPESIKFCKSLEIADFSGNPLSRLPEGFTQLRSLGHLALNDVSLQSLPSDIGNLANLVTLELRENLLKTLPTSLSFLVKLEQLDLGGNDLEVLPDTLGALPNLRELWLDRNQLSALPPELGNLRRLVCLDVSENKLEQLPNEVSGLVALTDLLLSQNLLECIPDGIGQLKQLSILKVDQNRLTEVTESIGDCENLSELILTENMLTALPKSLGKLAKLTNLNVDRNRLTSLPAEIGGCANLNVLSLRDNRLTLLPSELANTTELHVLDVAGNRLQNLPFALTNLNLKALWLAENQSQPMLKFQTEDDEKTGEKVLTCYLLPQQPSPSLENLLQNSVDESWTDTNLNRVSVIQFLDEPKADDEEESGAERRGLQRRATPHPSELKVMKKVIEVRRSEAHAAKPNADLNSPHSEEKRLSATSNRSEDSHLSNSTASADCRAEEQQEEAERSRPNGQVPEMQELEKEAKSRVDEEHKEAAGHEEEDVEVEYNEPTVHFAEDTLIRSEDEDEDEERPFPVEKQRLIRKDTPHYKKHFKITKLPKPEAVVALLQGLNSEGVPKEEEELGGCNNNTEPADQEEVWDEEDRKNGALSAQPSVKGVSFDQANNLLIEPARIEEEELTLTIVRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVRVGDKLLEVNGVSLHSAEHHVAVEALRGSGSSVSMTVLRERMVEPENAITVTPLRPEDDYSPRERRGGLRFPERPEGTPPTERYSTCLMRNEKGLGFSIAGGKGSTPYRAGDMGIFISRIAEGGAAHRDGILHVGDRVISINGVDMTEARHDQAVALLTASSPTIVLLVEREGAEQPGEGDWPGGPRARMHSPPPPPSHGESPPEETPAPQRNHLSKGLEDQYPIEEIHLVKAGGPLGLSIVGGSDHSSHPFGIHEPGVFISKVIPRGLASRSGLRVGDRILEVNSIDLRHATHQEAVNALLSNTQELTMLVRRDPPPPGMQEICIEKAPGEKLGISIRGGAKGHAGNPFDPTDEGIFISKVSSSGAAARDGRLKVGMRILEVNHQSLLGMTHTEAVQMLRGVGDALLVLVCDGFDPKAAAAIEMSPGIIANPFAAGIGRKNSLESISSIDRDLSPEELEILQKEMEMVREATQWEREEMEKVERMRREREAATRQLEEEAENVTSEPLRLDYRTLAALPSSGLQRVNRTLVQYNKPSSRMEGDCALLSLPVARDSRDSCPDTAAGPTIGSVARPPRDSATPDHCTSGAETPRPDQQPPTGGPLSQDQPPKTPEQECMVDSQPILFSENPFVVANRRGKAAGRACLGGPPLGYGRGGVLKTNLYSKAPAGESGMGNSRRETPYSPGNQQASINFITSQDDSKSTKPGVIQPLSRVRQNAAPHNSEEGDSPNPFQQPEPCFSVQNSPKPPSPPSPDEVPINVKQAYKTFAAVPLSHPLLGTQELPGQSGTENPRSAPEVAAHSPGGLHSPEQKSFRERQKYFEIEVKQQQMDKPPKRVSLVGEDDLKKMKEEEARKLQQKRTLLLEEEAEEEEMAKQAPEVSVQSSVIIEGVEYKIERLNGRSSQSPATRPSEVNENSSSQRNSLEEGIKAEQRTNSMSGLSPLYLGAGDAVAPVRTAKAERRHQERLRMQSPELLSGQEKELSPAERRALEAEKRAMWRAARSSALEDSIKQYEQDLARRLYQSRQRAPAPGAGPAPMPSPKQSHASAPPGSGSHSRMKSLEQDALKAQMVIAKSKEGKKRSPLEQLAESPSPVPTPSPTPLEDCSPRNVTSPGRLSMSEKKFDYREFAAIPSSKPVYEIQSPDAADDLRYIEDRNNSVPQEQDGQPEEEDTLGTHDSSTPTPSALEEMALYSSKRKLRHSRRSLEAAVPT

Target ORF information:

RefSeq Version	XM_014945715.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax scribbled planar cell polarity protein (SCRIB), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014945715.1

ORF Insert Sequence:

ATGCCGCCGC TCTGGCGCTG CAACCGGCAC GTGGAGGCGC TGGACCGGCG GCACTGCTCG 
CTGCAGGCCG TGCCCGAGGA GATCTACCGC TACAGCCGCT CGCTGGAGGA GCTGCTGCTG 
GACGCCAACC AGCTGCGGGA GCTGCCCAAG CCTTTCTTCA GGCTTCTGAA CCTGCGCAAG 
CTGGGCCTGA GTGACAATGA GATCCAAAGA CTTCCCCCTG AGGTGGCCAA CTTCATGCAG 
CTGGTGGAAC TGGACATCTC CCGTAACGAC ATTCCAGAAA TTCCTGAAAG CATCAAATTC 
TGCAAATCCT TGGAAATCGC AGACTTCAGT GGGAATCCTC TCTCCAGGCT TCCCGAGGGC 
TTCACGCAGC TTCGGAGCCT GGGCCATTTG GCCCTGAATG ATGTGTCCCT GCAGAGCCTC 
CCCAGTGACA TTGGGAATTT GGCAAACCTG GTGACTCTGG AGCTACGCGA GAACCTGCTA 
AAGACTCTCC CCACCTCGCT GTCCTTCCTT GTCAAGTTAG AGCAGCTGGA TCTCGGTGGC 
AATGACCTGG AGGTTCTGCC AGACACTCTG GGAGCGCTCC CCAACCTGCG TGAGCTCTGG 
TTAGACCGGA ACCAGCTCTC AGCCCTGCCT CCCGAGCTGG GCAACCTCCG CCGCCTGGTC 
TGCCTGGATG TGTCGGAGAA CAAGCTGGAG CAGTTGCCCA ACGAGGTCAG CGGCCTGGTG 
GCACTGACCG ACTTGCTGCT GTCGCAGAAC CTGTTGGAGT GCATTCCTGA TGGGATTGGT 
CAGCTGAAGC AGCTCTCCAT CCTCAAGGTG GACCAGAATC GACTGACAGA GGTGACAGAG 
TCCATTGGGG ACTGTGAAAA TCTGTCCGAG CTCATCCTGA CAGAGAACAT GCTGACGGCC 
TTGCCGAAAT CCCTTGGGAA GCTGGCCAAG CTGACCAACC TGAACGTGGA TCGGAACCGG 
CTGACATCCC TCCCGGCCGA GATCGGTGGC TGTGCCAACC TCAACGTGCT GTCCTTGCGG 
GACAATCGCC TGACCCTCCT GCCATCGGAG CTGGCCAACA CCACCGAGCT TCATGTTCTG 
GATGTGGCTG GGAACAGGCT GCAGAACTTG CCGTTTGCTC TGACAAACCT CAACCTGAAA 
GCCCTGTGGC TGGCGGAGAA TCAGTCCCAG CCCATGCTGA AATTCCAAAC AGAGGATGAT 
GAGAAGACAG GAGAGAAAGT TCTCACTTGT TACCTGCTTC CTCAGCAGCC CTCTCCCAGC 
CTGGAGAACC TTCTACAGAA CAGCGTGGAC GAGAGCTGGA CGGACACCAA TTTAAACAGA 
GTCAGTGTGA TTCAGTTCCT GGATGAACCC AAAGCAGATG ATGAAGAAGA GTCTGGAGCT 
GAGAGAAGGG GCTTGCAGCG CAGAGCAACC CCCCATCCCA GCGAGCTGAA GGTGATGAAG 
AAGGTGATTG AAGTTCGGCG CAGTGAGGCC CACGCTGCAA AGCCCAATGC AGACCTGAAT 
TCTCCTCACT CAGAAGAGAA GAGGCTGAGC GCCACGTCCA ACCGCAGCGA GGACTCCCAC 
CTTTCCAACA GCACTGCCTC TGCTGACTGT AGGGCGGAGG AGCAGCAGGA GGAGGCAGAG 
AGGAGTCGGC CAAACGGGCA AGTGCCTGAG ATGCAGGAGC TGGAGAAGGA GGCAAAGTCC 
AGAGTGGACG AGGAGCACAA GGAAGCTGCT GGCCATGAAG AGGAAGATGT GGAAGTGGAA 
TACAATGAGC CCACTGTACA CTTTGCTGAA GACACGCTAA TACGGAGTGA GGATGAAGAT 
GAAGATGAAG AGCGACCGTT CCCAGTGGAG AAGCAGCGGC TCATCCGCAA GGACACCCCT 
CACTATAAGA AACACTTTAA GATAACTAAA TTGCCCAAGC CAGAGGCGGT GGTGGCCCTG 
CTGCAGGGGC TGAACAGCGA GGGGGTCCCC AAGGAGGAAG AGGAGTTGGG AGGCTGCAAC 
AACAACACAG AGCCTGCAGA CCAGGAGGAG GTGTGGGATG AGGAGGACCG GAAGAATGGA 
GCTTTGTCTG CCCAGCCATC GGTGAAGGGG GTGTCGTTTG ATCAAGCCAA TAATCTGCTG 
ATTGAACCTG CTCGCATTGA GGAGGAAGAG TTGACTCTGA CTATCGTACG GCAGACGGGG 
GGGCTGGGCA TCAGCATCGC AGGGGGCAAG GGCTCCACCC CCTATAAGGG CGATGATGAG 
GGCATCTTTA TTTCTCGGGT GTCAGAAGAA GGTCCTGCGG CTCGTGCAGG GGTTCGTGTT 
GGGGACAAGC TCCTGGAGGT GAACGGTGTG TCCCTGCACT CCGCTGAGCA CCACGTGGCA 
GTGGAGGCTC TGCGTGGATC GGGAAGTTCC GTCTCCATGA CTGTTCTGCG GGAGCGGATG 
GTGGAGCCGG AGAATGCCAT CACTGTCACC CCGCTGCGCC CCGAGGATGA CTACAGCCCC 
CGTGAGAGGC GAGGTGGCCT GCGCTTCCCG GAGCGACCCG AGGGGACACC TCCCACCGAG 
AGATACTCCA CGTGCCTCAT GCGCAACGAG AAGGGACTGG GCTTCAGTAT CGCTGGTGGC 
AAAGGCTCCA CTCCTTACCG GGCTGGTGAC ATGGGGATCT TTATCTCCCG GATCGCAGAG 
GGTGGTGCAG CCCATCGGGA CGGGATCCTG CACGTAGGAG ATCGGGTCAT CTCGATCAAT 
GGGGTAGACA TGACAGAAGC CAGGCATGAT CAGGCAGTAG CGCTGCTTAC CGCGTCCTCC 
CCCACCATCG TGCTGCTGGT AGAGAGAGAG GGTGCGGAGC AGCCCGGCGA GGGAGACTGG 
CCCGGGGGGC CACGGGCACG CATGCACTCC CCACCGCCAC CCCCCAGCCA TGGGGAGAGC 
CCGCCCGAGG AGACGCCCGC ACCACAGAGG AACCATCTCT CCAAAGGCCT GGAGGATCAG 
TACCCCATTG AGGAAATTCA CCTGGTGAAA GCAGGTGGTC CCCTGGGGCT CAGCATCGTA 
GGGGGCAGTG ACCACTCAAG CCATCCGTTT GGGATTCATG AACCAGGTGT CTTCATTTCG 
AAGGTCATTC CCCGTGGGCT GGCATCCCGC AGTGGGCTGC GAGTAGGGGA CAGGATCCTG 
GAAGTCAATA GCATTGACCT GCGCCACGCT ACCCACCAGG AAGCGGTGAA CGCCCTGCTC 
TCCAACACCC AGGAGCTGAC CATGTTAGTG AGGCGAGACC CACCACCACC TGGTATGCAG 
GAAATATGCA TTGAGAAGGC TCCAGGAGAA AAGCTGGGCA TCAGCATCCG GGGTGGAGCG 
AAAGGCCATG CTGGAAATCC GTTTGATCCC ACTGACGAGG GCATCTTCAT CTCTAAGGTG 
AGCTCCTCGG GGGCGGCAGC CCGGGACGGC CGCCTGAAGG TGGGGATGCG GATCCTAGAG 
GTGAACCACC AGAGCCTGCT GGGGATGACG CACACGGAAG CGGTGCAGAT GCTGCGCGGG 
GTGGGCGACG CGCTCCTGGT GCTGGTGTGT GACGGCTTCG ACCCCAAGGC TGCAGCTGCC 
ATCGAGATGT CCCCGGGAAT TATTGCAAAC CCTTTCGCTG CTGGTATCGG ACGCAAGAAT 
AGCCTGGAAA GTATCTCTTC CATCGACCGG GATTTAAGCC CTGAGGAACT GGAGATCCTG 
CAGAAGGAGA TGGAAATGGT GAGAGAAGCA ACTCAGTGGG AGAGAGAAGA AATGGAGAAA 
GTGGAGCGGA TGCGTAGGGA GCGGGAGGCA GCCACCCGGC AGCTTGAGGA GGAGGCGGAG 
AACGTCACCA GCGAGCCCTT GAGACTGGAC TATCGGACCC TGGCTGCGCT GCCGAGCAGT 
GGTCTCCAGA GAGTCAACCG CACCCTCGTT CAGTATAATA AGCCCAGCTC CAGAATGGAG 
GGCGACTGTG CTCTGCTGTC TCTGCCGGTG GCACGGGACA GCCGGGATTC ATGCCCTGAC 
ACGGCTGCTG GCCCCACCAT CGGAAGCGTG GCCAGACCTC CCCGGGACTC CGCAACACCC 
GACCACTGCA CCAGCGGGGC AGAAACGCCC CGGCCTGACC AGCAGCCTCC CACAGGTGGC 
CCCTTGTCCC AGGACCAGCC GCCAAAGACG CCAGAGCAAG AGTGCATGGT GGACTCCCAG 
CCCATCCTCT TCAGTGAGAA CCCCTTTGTG GTGGCCAACC GCAGGGGGAA GGCGGCCGGC 
CGGGCCTGCC TGGGGGGGCC TCCCCTGGGC TATGGCAGGG GGGGAGTGCT GAAGACCAAC 
CTTTACTCCA AGGCTCCTGC AGGCGAGTCT GGGATGGGCA ATTCCAGGCG TGAAACCCCC 
TATTCTCCTG GTAACCAGCA GGCTTCCATT AACTTCATTA CATCGCAGGA TGACAGTAAA 
TCGACCAAGC CAGGCGTCAT TCAGCCTCTT TCCCGAGTGC GACAAAATGC TGCCCCGCAC 
AATTCGGAGG AGGGGGACAG CCCCAATCCC TTCCAGCAGC CCGAGCCGTG CTTCAGTGTC 
CAGAACTCTC CAAAGCCACC CTCACCACCC TCTCCTGATG AGGTCCCCAT CAATGTCAAG 
CAAGCGTACA AGACCTTCGC TGCAGTGCCC TTGTCACACC CCCTGCTTGG GACACAGGAA 
TTGCCTGGTC AGAGCGGCAC AGAGAATCCC AGAAGTGCCC CGGAGGTGGC TGCGCACAGC 
CCTGGGGGAC TGCACAGCCC TGAGCAGAAG TCCTTCCGTG AGAGGCAGAA GTATTTCGAG 
ATTGAGGTGA AGCAGCAGCA GATGGACAAG CCCCCCAAGA GAGTCTCCTT GGTGGGAGAG 
GACGACCTGA AGAAGATGAA AGAGGAGGAA GCTCGAAAAC TGCAGCAGAA ACGTACCCTT 
CTGCTGGAGG AAGAGGCAGA AGAGGAGGAG ATGGCGAAGC AGGCGCCTGA GGTGAGCGTG 
CAGTCCAGTG TCATCATTGA AGGAGTCGAG TACAAGATTG AGAGACTCAA CGGGAGGAGC 
AGCCAGTCTC CGGCCACTCG GCCCTCCGAG GTCAACGAGA ACAGCAGCTC GCAGAGGAAT 
TCACTGGAAG AGGGGATCAA GGCTGAGCAG AGAACCAACT CCATGTCTGG GCTGAGTCCC 
TTGTACCTTG GAGCAGGGGA CGCAGTGGCA CCCGTGCGGA CGGCCAAAGC TGAACGGCGA 
CACCAGGAGC GGTTGCGAAT GCAGAGTCCT GAGCTGCTGA GTGGCCAAGA GAAGGAGCTT 
TCTCCTGCAG AACGTCGTGC TCTGGAGGCG GAGAAACGAG CAATGTGGAG GGCAGCACGA 
TCCTCAGCTC TTGAAGACAG TATTAAACAG TACGAGCAGG ACCTGGCCAG GAGGCTGTAC 
CAGTCCCGAC AGCGGGCACC TGCTCCCGGG GCCGGGCCAG CTCCGATGCC TAGTCCCAAG 
CAGAGTCACG CCTCGGCACC CCCTGGGTCA GGAAGCCATT CCAGGATGAA ATCGCTTGAA 
CAGGATGCTC TGAAAGCCCA GATGGTCATT GCCAAGTCCA AGGAGGGGAA GAAGCGCAGC 
CCGCTGGAGC AGCTGGCAGA GTCCCCCTCG CCAGTTCCTA CACCGTCACC GACACCACTG 
GAAGATTGCA GTCCCAGGAA CGTCACCTCC CCAGGAAGGC TGTCCATGTC TGAAAAGAAG 
TTTGACTACC GGGAATTTGC TGCTATTCCT TCCTCCAAAC CCGTTTACGA AATTCAGTCG 
CCTGATGCTG CTGATGACCT CAGATACATC GAGGACAGGA ATAACTCAGT TCCCCAGGAG 
CAGGATGGGC AGCCCGAGGA AGAGGACACA TTAGGAACTC ACGACTCATC CACACCCACT 
CCATCAGCAC TTGAAGAAAT GGCCCTGTAC AGCAGCAAAC GCAAGCTCCG ACACAGCCGG 
CGCAGCCTGG AGGCTGCCGT CCCTACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCRIB ( XM_014945716.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014801202.1 Calidris pugnax scribbled planar cell polarity protein (SCRIB), transcript variant X3, mRNA.

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CloneID	OCa67749
Clone ID Related Accession (Same CDS sequence)	XM_014945716.1
Accession Version	XM_014945716.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5904bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	protein scribble homolog isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090871.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCCGC TCTGGCGCTG CAACCGGCAC GTGGAGGCGC TGGACCGGCG GCACTGCTCG 
CTGCAGGCCG TGCCCGAGGA GATCTACCGC TACAGCCGCT CGCTGGAGGA GCTGCTGCTG 
GACGCCAACC AGCTGCGGGA GCTGCCCAAG CCTTTCTTCA GGCTTCTGAA CCTGCGCAAG 
CTGGGCCTGA GTGACAATGA GATCCAAAGA CTTCCCCCTG AGGTGGCCAA CTTCATGCAG 
CTGGTGGAAC TGGACATCTC CCGTAACGAC ATTCCAGAAA TTCCTGAAAG CATCAAATTC 
TGCAAATCCT TGGAAATCGC AGACTTCAGT GGGAATCCTC TCTCCAGGCT TCCCGAGGGC 
TTCACGCAGC TTCGGAGCCT GGGCCATTTG GCCCTGAATG ATGTGTCCCT GCAGAGCCTC 
CCCAGTGACA TTGGGAATTT GGCAAACCTG GTGACTCTGG AGCTACGCGA GAACCTGCTA 
AAGACTCTCC CCACCTCGCT GTCCTTCCTT GTCAAGTTAG AGCAGCTGGA TCTCGGTGGC 
AATGACCTGG AGGTTCTGCC AGACACTCTG GGAGCGCTCC CCAACCTGCG TGAGCTCTGG 
TTAGACCGGA ACCAGCTCTC AGCCCTGCCT CCCGAGCTGG GCAACCTCCG CCGCCTGGTC 
TGCCTGGATG TGTCGGAGAA CAAGCTGGAG CAGTTGCCCA ACGAGGTCAG CGGCCTGGTG 
GCACTGACCG ACTTGCTGCT GTCGCAGAAC CTGTTGGAGT GCATTCCTGA TGGGATTGGT 
CAGCTGAAGC AGCTCTCCAT CCTCAAGGTG GACCAGAATC GACTGACAGA GGTGACAGAG 
TCCATTGGGG ACTGTGAAAA TCTGTCCGAG CTCATCCTGA CAGAGAACAT GCTGACGGCC 
TTGCCGAAAT CCCTTGGGAA GCTGGCCAAG CTGACCAACC TGAACGTGGA TCGGAACCGG 
CTGACATCCC TCCCGGCCGA GATCGGTGGC TGTGCCAACC TCAACGTGCT GTCCTTGCGG 
GACAATCGCC TGACCCTCCT GCCATCGGAG CTGGCCAACA CCACCGAGCT TCATGTTCTG 
GATGTGGCTG GGAACAGGCT GCAGAACTTG CCGTTTGCTC TGACAAACCT CAACCTGAAA 
GCCCTGTGGC TGGCGGAGAA TCAGTCCCAG CCCATGCTGA AATTCCAAAC AGAGGATGAT 
GAGAAGACAG GAGAGAAAGT TCTCACTTGT TACCTGCTTC CTCAGCAGCC CTCTCCCAGC 
CTGGAGAACC TTCTACAGAA CAGCGTGGAC GAGAGCTGGA CGGACACCAA TTTAAACAGA 
GTCAGTGTGA TTCAGTTCCT GGATGAACCC AAAGCAGATG ATGAAGAAGA GTCTGGAGCT 
GAGAGAAGGG GCTTGCAGCG CAGAGCAACC CCCCATCCCA GCGAGCTGAA GGTGATGAAG 
AAGGTGATTG AAGTTCGGCG CAGTGAGGCC CACGCTGCAA AGCCCAATGC AGACCTGAAT 
TCTCCTCACT CAGAAGAGAA GAGGCTGAGC GCCACGTCCA ACCGCAGCGA GGACTCCCAC 
CTTTCCAACA GCACTGCCTC TGCTGACTGT AGGGCGGAGG AGCAGCAGGA GGAGGCAGAG 
AGGAGTCGGC CAAACGGGCA AGTGCCTGAG ATGCAGGAGC TGGAGAAGGA GGCAAAGTCC 
AGAGTGGACG AGGAGCACAA GGAAGCTGCT GGCCATGAAG AGGAAGATGT GGAAGTGGAA 
TACAATGAGC CCACTGTACA CTTTGCTGAA GACACGCTAA TACGGAGTGA GGATGAAGAT 
GAAGATGAAG AGCGACCGTT CCCAGTGGAG AAGCAGCGGC TCATCCGCAA GGACACCCCT 
CACTATAAGA AACACTTTAA GATAACTAAA TTGCCCAAGC CAGAGGCGGT GGTGGCCCTG 
CTGCAGGGGC TGAACAGCGA GGGGGTCCCC AAGGAGGAAG AGGAGTTGGG AGGCTGCAAC 
AACAACACAG AGCCTGCAGA CCAGGAGGAG GTGTGGGATG AGGAGGACCG GAAGAATGGA 
GCTTTGTCTG CCCAGCCATC GGTGAAGGGG GTGTCGTTTG ATCAAGCCAA TAATCTGCTG 
ATTGAACCTG CTCGCATTGA GGAGGAAGAG TTGACTCTGA CTATCGTACG GCAGACGGGG 
GGGCTGGGCA TCAGCATCGC AGGGGGCAAG GGCTCCACCC CCTATAAGGG CGATGATGAG 
GGCATCTTTA TTTCTCGGGT GTCAGAAGAA GGTCCTGCGG CTCGTGCAGG GGTTCGTGTT 
GGGGACAAGC TCCTGGAGGT GAACGGTGTG TCCCTGCACT CCGCTGAGCA CCACGTGGCA 
GTGGAGGCTC TGCGTGGATC GGGAAGTTCC GTCTCCATGA CTGTTCTGCG GGAGCGGATG 
GTGGAGCCGG AGAATGCCAT CACTGTCACC CCGCTGCGCC CCGAGGATGA CTACAGCCCC 
CGTGAGAGGC GAGGTGGCCT GCGCTTCCCG GAGCGACCCG AGGGGACACC TCCCACCGAG 
AGATACTCCA CGTGCCTCAT GCGCAACGAG AAGGGACTGG GCTTCAGTAT CGCTGGTGGC 
AAAGGCTCCA CTCCTTACCG GGCTGGTGAC ATGGGGATCT TTATCTCCCG GATCGCAGAG 
GGTGGTGCAG CCCATCGGGA CGGGATCCTG CACGTAGGAG ATCGGGTCAT CTCGATCAAT 
GGGGTAGACA TGACAGAAGC CAGGCATGAT CAGGCAGTAG CGCTGCTTAC CGCGTCCTCC 
CCCACCATCG TGCTGCTGGT AGAGAGAGAG GGTGCGGAGC AGCCCGGCGA GGGAGACTGG 
CCCGGGGGGC CACGGGCACG CATGCACTCC CCACCGCCAC CCCCCAGCCA TGGGGAGAGC 
CCGCCCGAGG AGACGCCCGC ACCACAGAGG AACCATCTCT CCAAAGGCCT GGAGGATCAG 
TACCCCATTG AGGAAATTCA CCTGGTGAAA GCAGGTGGTC CCCTGGGGCT CAGCATCGTA 
GGGGGCAGTG ACCACTCAAG CCATCCGTTT GGGATTCATG AACCAGGTGT CTTCATTTCG 
AAGGTCATTC CCCGTGGGCT GGCATCCCGC AGTGGGCTGC GAGTAGGGGA CAGGATCCTG 
GAAGTCAATA GCATTGACCT GCGCCACGCT ACCCACCAGG AAGCGGTGAA CGCCCTGCTC 
TCCAACACCC AGGAGCTGAC CATGTTAGTG AGGCGAGACC CACCACCACC TGGTATGCAG 
GAAATATGCA TTGAGAAGGC TCCAGGAGAA AAGCTGGGCA TCAGCATCCG GGGTGGAGCG 
AAAGGCCATG CTGGAAATCC GTTTGATCCC ACTGACGAGG GCATCTTCAT CTCTAAGGTG 
AGCTCCTCGG GGGCGGCAGC CCGGGACGGC CGCCTGAAGG TGGGGATGCG GATCCTAGAG 
GTGAACCACC AGAGCCTGCT GGGGATGACG CACACGGAAG CGGTGCAGAT GCTGCGCGGG 
GTGGGCGACG CGCTCCTGGT GCTGGTGTGT GACGGCTTCG ACCCCAAGGC TGCAGCTGCC 
ATCGAGATGT CCCCGGGAAT TATTGCAAAC CCTTTCGCTG CTGGTATCGG ACGCAAGAAT 
AGCCTGGAAA GTATCTCTTC CATCGACCGG GATTTAAGCC CTGAGGAACT GGAGATCCTG 
CAGAAGGAGA TGGAAATGGT GAGAGAAGCA ACTCAGTGGG AGAGAGAAGA AATGGAGAAA 
GTGAACGTCA CCAGCGAGCC CTTGAGACTG GACTATCGGA CCCTGGCTGC GCTGCCGAGC 
AGTGGTCTCC AGAGAGTCAA CCGCACCCTC GTTCAGTATA ATAAGCCCAG CTCCAGAATG 
GAGGGCGACT GTGCTCTGCT GTCTCTGCCG GTGGCACGGG ACAGCCGGGA TTCATGCCCT 
GACACGGCTG CTGGCCCCAC CATCGGAAGC GTGGCCAGAC CTCCCCGGGA CTCCGCAACA 
CCCGACCACT GCACCAGCGG GGCAGAAACG CCCCGGCCTG ACCAGCAGCC TCCCACAGGT 
GGCCCCTTGT CCCAGGACCA GCCGCCAAAG ACGCCAGAGC AAGAGTGCAT GGTGGACTCC 
CAGCCCATCC TCTTCAGTGA GAACCCCTTT GTGGTGGCCA ACCGCAGGGG GAAGGCGGCC 
GGCCGGGCCT GCCTGGGGGG GCCTCCCCTG GGCTATGGCA GGGGGGGAGT GCTGAAGACC 
AACCTTTACT CCAAGGCTCC TGCAGGCGAG TCTGGGATGG GCAATTCCAG GCGTGAAACC 
CCCTATTCTC CTGGTAACCA GCAGCGGCCT GCAACCCCTC GGCCCGCTCA CCTGCCGGGC 
AGGGAGACCC GCTTTGCTTC CATTAACTTC ATTACATCGC AGGATGACAG TAAATCGACC 
AAGCCAGGCG TCATTCAGCC TCTTTCCCGA GTGCGACAAA ATGCTGCCCC GCACAATTCG 
GAGGAGGGGG ACAGCCCCAA TCCCTTCCAG CAGCCCGAGC CGTGCTTCAG TGTCCAGAAC 
TCTCCAAAGC CACCCTCACC ACCCTCTCCT GATGAGGTCC CCATCAATGT CAAGCAAGCG 
TACAAGACCT TCGCTGCAGT GCCCTTGTCA CACCCCCTGC TTGGGACACA GGAATTGCCT 
GGTCAGAGCG GCACAGAGAA TCCCAGAAGT GCCCCGGAGG TGGCTGCGCA CAGCCCTGGG 
GGACTGCACA GCCCTGAGCA GAAGTCCTTC CGTGAGAGGC AGAAGTATTT CGAGATTGAG 
GTGAAGCAGC AGCAGATGGA CAAGCCCCCC AAGAGAGTCT CCTTGGTGGG AGAGGACGAC 
CTGAAGAAGA TGAAAGAGGA GGAAGCTCGA AAACTGCAGC AGAAACGTAC CCTTCTGCTG 
GAGGAAGAGG CAGAAGAGGA GGAGATGGCG AAGCAGGCGC CTGAGGTGAG CGTGCAGTCC 
AGTGTCATCA TTGAAGGAGT CGAGTACAAG ATTGAGAGAC TCAACGGGAG GAGCAGCCAG 
TCTCCGGCCA CTCGGCCCTC CGAGGTCAAC GAGAACAGCA GCTCGCAGAG GAATTCACTG 
GAAGAGGGGA TCAAGGCTGA GCAGAGAACC AACTCCATGT CTGGGCTGAG TCCCTTGTAC 
CTTGGAGCAG GGGACGCAGT GGCACCCGTG CGGACGGCCA AAGCTGAACG GCGACACCAG 
GAGCGGTTGC GAATGCAGAG TCCTGAGCTG CTGAGTGGCC AAGAGAAGGA GCTTTCTCCT 
GCAGAACGTC GTGCTCTGGA GGCGGAGAAA CGAGCAATGT GGAGGGCAGC ACGATCCTCA 
GCTCTTGAAG ACAGTATTAA ACAGTACGAG CAGGACCTGG CCAGGAGGCT GTACCAGTCC 
CGACAGCGGG CACCTGCTCC CGGGGCCGGG CCAGCTCCGA TGCCTAGTCC CAAGCAGAGT 
CACGCCTCGG CACCCCCTGG GTCAGGAAGC CATTCCAGGA TGAAATCGCT TGAACAGGAT 
GCTCTGAAAG CCCAGATGGT CATTGCCAAG TCCAAGGAGG GGAAGAAGCG CAGCCCGCTG 
GAGCAGCTGG CAGAGTCCCC CTCGCCAGTT CCTACACCGT CACCGACACC ACTGGAAGAT 
TGCAGTCCCA GGAACGTCAC CTCCCCAGGA AGGCTGTCCA TGTCTGAAAA GAAGTTTGAC 
TACCGGGAAT TTGCTGCTAT TCCTTCCTCC AAACCCGTTT ACGAAATTCA GTCGCCTGAT 
GCTGCTGATG ACCTCAGATA CATCGAGGAC AGGAATAACT CAGTTCCCCA GGAGCAGGAT 
GGGCAGCCCG AGGAAGAGGA CACATTAGGA ACTCACGACT CATCCACACC CACTCCATCA 
GCACTTGAAG AAATGGCCCT GTACAGCAGC AAACGCAAGC TCCGACACAG CCGGCGCAGC 
CTGGAGGCTG CCGTCCCTAC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014801202.1
CDS	6..5909
Translation	MPPLWRCNRHVEALDRRHCSLQAVPEEIYRYSRSLEELLLDANQLRELPKPFFRLLNLRKLGLSDNEIQRLPPEVANFMQLVELDISRNDIPEIPESIKFCKSLEIADFSGNPLSRLPEGFTQLRSLGHLALNDVSLQSLPSDIGNLANLVTLELRENLLKTLPTSLSFLVKLEQLDLGGNDLEVLPDTLGALPNLRELWLDRNQLSALPPELGNLRRLVCLDVSENKLEQLPNEVSGLVALTDLLLSQNLLECIPDGIGQLKQLSILKVDQNRLTEVTESIGDCENLSELILTENMLTALPKSLGKLAKLTNLNVDRNRLTSLPAEIGGCANLNVLSLRDNRLTLLPSELANTTELHVLDVAGNRLQNLPFALTNLNLKALWLAENQSQPMLKFQTEDDEKTGEKVLTCYLLPQQPSPSLENLLQNSVDESWTDTNLNRVSVIQFLDEPKADDEEESGAERRGLQRRATPHPSELKVMKKVIEVRRSEAHAAKPNADLNSPHSEEKRLSATSNRSEDSHLSNSTASADCRAEEQQEEAERSRPNGQVPEMQELEKEAKSRVDEEHKEAAGHEEEDVEVEYNEPTVHFAEDTLIRSEDEDEDEERPFPVEKQRLIRKDTPHYKKHFKITKLPKPEAVVALLQGLNSEGVPKEEEELGGCNNNTEPADQEEVWDEEDRKNGALSAQPSVKGVSFDQANNLLIEPARIEEEELTLTIVRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVRVGDKLLEVNGVSLHSAEHHVAVEALRGSGSSVSMTVLRERMVEPENAITVTPLRPEDDYSPRERRGGLRFPERPEGTPPTERYSTCLMRNEKGLGFSIAGGKGSTPYRAGDMGIFISRIAEGGAAHRDGILHVGDRVISINGVDMTEARHDQAVALLTASSPTIVLLVEREGAEQPGEGDWPGGPRARMHSPPPPPSHGESPPEETPAPQRNHLSKGLEDQYPIEEIHLVKAGGPLGLSIVGGSDHSSHPFGIHEPGVFISKVIPRGLASRSGLRVGDRILEVNSIDLRHATHQEAVNALLSNTQELTMLVRRDPPPPGMQEICIEKAPGEKLGISIRGGAKGHAGNPFDPTDEGIFISKVSSSGAAARDGRLKVGMRILEVNHQSLLGMTHTEAVQMLRGVGDALLVLVCDGFDPKAAAAIEMSPGIIANPFAAGIGRKNSLESISSIDRDLSPEELEILQKEMEMVREATQWEREEMEKVNVTSEPLRLDYRTLAALPSSGLQRVNRTLVQYNKPSSRMEGDCALLSLPVARDSRDSCPDTAAGPTIGSVARPPRDSATPDHCTSGAETPRPDQQPPTGGPLSQDQPPKTPEQECMVDSQPILFSENPFVVANRRGKAAGRACLGGPPLGYGRGGVLKTNLYSKAPAGESGMGNSRRETPYSPGNQQRPATPRPAHLPGRETRFASINFITSQDDSKSTKPGVIQPLSRVRQNAAPHNSEEGDSPNPFQQPEPCFSVQNSPKPPSPPSPDEVPINVKQAYKTFAAVPLSHPLLGTQELPGQSGTENPRSAPEVAAHSPGGLHSPEQKSFRERQKYFEIEVKQQQMDKPPKRVSLVGEDDLKKMKEEEARKLQQKRTLLLEEEAEEEEMAKQAPEVSVQSSVIIEGVEYKIERLNGRSSQSPATRPSEVNENSSSQRNSLEEGIKAEQRTNSMSGLSPLYLGAGDAVAPVRTAKAERRHQERLRMQSPELLSGQEKELSPAERRALEAEKRAMWRAARSSALEDSIKQYEQDLARRLYQSRQRAPAPGAGPAPMPSPKQSHASAPPGSGSHSRMKSLEQDALKAQMVIAKSKEGKKRSPLEQLAESPSPVPTPSPTPLEDCSPRNVTSPGRLSMSEKKFDYREFAAIPSSKPVYEIQSPDAADDLRYIEDRNNSVPQEQDGQPEEEDTLGTHDSSTPTPSALEEMALYSSKRKLRHSRRSLEAAVPT

Target ORF information:

RefSeq Version	XM_014945716.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax scribbled planar cell polarity protein (SCRIB), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014945716.1

ORF Insert Sequence:

ATGCCGCCGC TCTGGCGCTG CAACCGGCAC GTGGAGGCGC TGGACCGGCG GCACTGCTCG 
CTGCAGGCCG TGCCCGAGGA GATCTACCGC TACAGCCGCT CGCTGGAGGA GCTGCTGCTG 
GACGCCAACC AGCTGCGGGA GCTGCCCAAG CCTTTCTTCA GGCTTCTGAA CCTGCGCAAG 
CTGGGCCTGA GTGACAATGA GATCCAAAGA CTTCCCCCTG AGGTGGCCAA CTTCATGCAG 
CTGGTGGAAC TGGACATCTC CCGTAACGAC ATTCCAGAAA TTCCTGAAAG CATCAAATTC 
TGCAAATCCT TGGAAATCGC AGACTTCAGT GGGAATCCTC TCTCCAGGCT TCCCGAGGGC 
TTCACGCAGC TTCGGAGCCT GGGCCATTTG GCCCTGAATG ATGTGTCCCT GCAGAGCCTC 
CCCAGTGACA TTGGGAATTT GGCAAACCTG GTGACTCTGG AGCTACGCGA GAACCTGCTA 
AAGACTCTCC CCACCTCGCT GTCCTTCCTT GTCAAGTTAG AGCAGCTGGA TCTCGGTGGC 
AATGACCTGG AGGTTCTGCC AGACACTCTG GGAGCGCTCC CCAACCTGCG TGAGCTCTGG 
TTAGACCGGA ACCAGCTCTC AGCCCTGCCT CCCGAGCTGG GCAACCTCCG CCGCCTGGTC 
TGCCTGGATG TGTCGGAGAA CAAGCTGGAG CAGTTGCCCA ACGAGGTCAG CGGCCTGGTG 
GCACTGACCG ACTTGCTGCT GTCGCAGAAC CTGTTGGAGT GCATTCCTGA TGGGATTGGT 
CAGCTGAAGC AGCTCTCCAT CCTCAAGGTG GACCAGAATC GACTGACAGA GGTGACAGAG 
TCCATTGGGG ACTGTGAAAA TCTGTCCGAG CTCATCCTGA CAGAGAACAT GCTGACGGCC 
TTGCCGAAAT CCCTTGGGAA GCTGGCCAAG CTGACCAACC TGAACGTGGA TCGGAACCGG 
CTGACATCCC TCCCGGCCGA GATCGGTGGC TGTGCCAACC TCAACGTGCT GTCCTTGCGG 
GACAATCGCC TGACCCTCCT GCCATCGGAG CTGGCCAACA CCACCGAGCT TCATGTTCTG 
GATGTGGCTG GGAACAGGCT GCAGAACTTG CCGTTTGCTC TGACAAACCT CAACCTGAAA 
GCCCTGTGGC TGGCGGAGAA TCAGTCCCAG CCCATGCTGA AATTCCAAAC AGAGGATGAT 
GAGAAGACAG GAGAGAAAGT TCTCACTTGT TACCTGCTTC CTCAGCAGCC CTCTCCCAGC 
CTGGAGAACC TTCTACAGAA CAGCGTGGAC GAGAGCTGGA CGGACACCAA TTTAAACAGA 
GTCAGTGTGA TTCAGTTCCT GGATGAACCC AAAGCAGATG ATGAAGAAGA GTCTGGAGCT 
GAGAGAAGGG GCTTGCAGCG CAGAGCAACC CCCCATCCCA GCGAGCTGAA GGTGATGAAG 
AAGGTGATTG AAGTTCGGCG CAGTGAGGCC CACGCTGCAA AGCCCAATGC AGACCTGAAT 
TCTCCTCACT CAGAAGAGAA GAGGCTGAGC GCCACGTCCA ACCGCAGCGA GGACTCCCAC 
CTTTCCAACA GCACTGCCTC TGCTGACTGT AGGGCGGAGG AGCAGCAGGA GGAGGCAGAG 
AGGAGTCGGC CAAACGGGCA AGTGCCTGAG ATGCAGGAGC TGGAGAAGGA GGCAAAGTCC 
AGAGTGGACG AGGAGCACAA GGAAGCTGCT GGCCATGAAG AGGAAGATGT GGAAGTGGAA 
TACAATGAGC CCACTGTACA CTTTGCTGAA GACACGCTAA TACGGAGTGA GGATGAAGAT 
GAAGATGAAG AGCGACCGTT CCCAGTGGAG AAGCAGCGGC TCATCCGCAA GGACACCCCT 
CACTATAAGA AACACTTTAA GATAACTAAA TTGCCCAAGC CAGAGGCGGT GGTGGCCCTG 
CTGCAGGGGC TGAACAGCGA GGGGGTCCCC AAGGAGGAAG AGGAGTTGGG AGGCTGCAAC 
AACAACACAG AGCCTGCAGA CCAGGAGGAG GTGTGGGATG AGGAGGACCG GAAGAATGGA 
GCTTTGTCTG CCCAGCCATC GGTGAAGGGG GTGTCGTTTG ATCAAGCCAA TAATCTGCTG 
ATTGAACCTG CTCGCATTGA GGAGGAAGAG TTGACTCTGA CTATCGTACG GCAGACGGGG 
GGGCTGGGCA TCAGCATCGC AGGGGGCAAG GGCTCCACCC CCTATAAGGG CGATGATGAG 
GGCATCTTTA TTTCTCGGGT GTCAGAAGAA GGTCCTGCGG CTCGTGCAGG GGTTCGTGTT 
GGGGACAAGC TCCTGGAGGT GAACGGTGTG TCCCTGCACT CCGCTGAGCA CCACGTGGCA 
GTGGAGGCTC TGCGTGGATC GGGAAGTTCC GTCTCCATGA CTGTTCTGCG GGAGCGGATG 
GTGGAGCCGG AGAATGCCAT CACTGTCACC CCGCTGCGCC CCGAGGATGA CTACAGCCCC 
CGTGAGAGGC GAGGTGGCCT GCGCTTCCCG GAGCGACCCG AGGGGACACC TCCCACCGAG 
AGATACTCCA CGTGCCTCAT GCGCAACGAG AAGGGACTGG GCTTCAGTAT CGCTGGTGGC 
AAAGGCTCCA CTCCTTACCG GGCTGGTGAC ATGGGGATCT TTATCTCCCG GATCGCAGAG 
GGTGGTGCAG CCCATCGGGA CGGGATCCTG CACGTAGGAG ATCGGGTCAT CTCGATCAAT 
GGGGTAGACA TGACAGAAGC CAGGCATGAT CAGGCAGTAG CGCTGCTTAC CGCGTCCTCC 
CCCACCATCG TGCTGCTGGT AGAGAGAGAG GGTGCGGAGC AGCCCGGCGA GGGAGACTGG 
CCCGGGGGGC CACGGGCACG CATGCACTCC CCACCGCCAC CCCCCAGCCA TGGGGAGAGC 
CCGCCCGAGG AGACGCCCGC ACCACAGAGG AACCATCTCT CCAAAGGCCT GGAGGATCAG 
TACCCCATTG AGGAAATTCA CCTGGTGAAA GCAGGTGGTC CCCTGGGGCT CAGCATCGTA 
GGGGGCAGTG ACCACTCAAG CCATCCGTTT GGGATTCATG AACCAGGTGT CTTCATTTCG 
AAGGTCATTC CCCGTGGGCT GGCATCCCGC AGTGGGCTGC GAGTAGGGGA CAGGATCCTG 
GAAGTCAATA GCATTGACCT GCGCCACGCT ACCCACCAGG AAGCGGTGAA CGCCCTGCTC 
TCCAACACCC AGGAGCTGAC CATGTTAGTG AGGCGAGACC CACCACCACC TGGTATGCAG 
GAAATATGCA TTGAGAAGGC TCCAGGAGAA AAGCTGGGCA TCAGCATCCG GGGTGGAGCG 
AAAGGCCATG CTGGAAATCC GTTTGATCCC ACTGACGAGG GCATCTTCAT CTCTAAGGTG 
AGCTCCTCGG GGGCGGCAGC CCGGGACGGC CGCCTGAAGG TGGGGATGCG GATCCTAGAG 
GTGAACCACC AGAGCCTGCT GGGGATGACG CACACGGAAG CGGTGCAGAT GCTGCGCGGG 
GTGGGCGACG CGCTCCTGGT GCTGGTGTGT GACGGCTTCG ACCCCAAGGC TGCAGCTGCC 
ATCGAGATGT CCCCGGGAAT TATTGCAAAC CCTTTCGCTG CTGGTATCGG ACGCAAGAAT 
AGCCTGGAAA GTATCTCTTC CATCGACCGG GATTTAAGCC CTGAGGAACT GGAGATCCTG 
CAGAAGGAGA TGGAAATGGT GAGAGAAGCA ACTCAGTGGG AGAGAGAAGA AATGGAGAAA 
GTGAACGTCA CCAGCGAGCC CTTGAGACTG GACTATCGGA CCCTGGCTGC GCTGCCGAGC 
AGTGGTCTCC AGAGAGTCAA CCGCACCCTC GTTCAGTATA ATAAGCCCAG CTCCAGAATG 
GAGGGCGACT GTGCTCTGCT GTCTCTGCCG GTGGCACGGG ACAGCCGGGA TTCATGCCCT 
GACACGGCTG CTGGCCCCAC CATCGGAAGC GTGGCCAGAC CTCCCCGGGA CTCCGCAACA 
CCCGACCACT GCACCAGCGG GGCAGAAACG CCCCGGCCTG ACCAGCAGCC TCCCACAGGT 
GGCCCCTTGT CCCAGGACCA GCCGCCAAAG ACGCCAGAGC AAGAGTGCAT GGTGGACTCC 
CAGCCCATCC TCTTCAGTGA GAACCCCTTT GTGGTGGCCA ACCGCAGGGG GAAGGCGGCC 
GGCCGGGCCT GCCTGGGGGG GCCTCCCCTG GGCTATGGCA GGGGGGGAGT GCTGAAGACC 
AACCTTTACT CCAAGGCTCC TGCAGGCGAG TCTGGGATGG GCAATTCCAG GCGTGAAACC 
CCCTATTCTC CTGGTAACCA GCAGCGGCCT GCAACCCCTC GGCCCGCTCA CCTGCCGGGC 
AGGGAGACCC GCTTTGCTTC CATTAACTTC ATTACATCGC AGGATGACAG TAAATCGACC 
AAGCCAGGCG TCATTCAGCC TCTTTCCCGA GTGCGACAAA ATGCTGCCCC GCACAATTCG 
GAGGAGGGGG ACAGCCCCAA TCCCTTCCAG CAGCCCGAGC CGTGCTTCAG TGTCCAGAAC 
TCTCCAAAGC CACCCTCACC ACCCTCTCCT GATGAGGTCC CCATCAATGT CAAGCAAGCG 
TACAAGACCT TCGCTGCAGT GCCCTTGTCA CACCCCCTGC TTGGGACACA GGAATTGCCT 
GGTCAGAGCG GCACAGAGAA TCCCAGAAGT GCCCCGGAGG TGGCTGCGCA CAGCCCTGGG 
GGACTGCACA GCCCTGAGCA GAAGTCCTTC CGTGAGAGGC AGAAGTATTT CGAGATTGAG 
GTGAAGCAGC AGCAGATGGA CAAGCCCCCC AAGAGAGTCT CCTTGGTGGG AGAGGACGAC 
CTGAAGAAGA TGAAAGAGGA GGAAGCTCGA AAACTGCAGC AGAAACGTAC CCTTCTGCTG 
GAGGAAGAGG CAGAAGAGGA GGAGATGGCG AAGCAGGCGC CTGAGGTGAG CGTGCAGTCC 
AGTGTCATCA TTGAAGGAGT CGAGTACAAG ATTGAGAGAC TCAACGGGAG GAGCAGCCAG 
TCTCCGGCCA CTCGGCCCTC CGAGGTCAAC GAGAACAGCA GCTCGCAGAG GAATTCACTG 
GAAGAGGGGA TCAAGGCTGA GCAGAGAACC AACTCCATGT CTGGGCTGAG TCCCTTGTAC 
CTTGGAGCAG GGGACGCAGT GGCACCCGTG CGGACGGCCA AAGCTGAACG GCGACACCAG 
GAGCGGTTGC GAATGCAGAG TCCTGAGCTG CTGAGTGGCC AAGAGAAGGA GCTTTCTCCT 
GCAGAACGTC GTGCTCTGGA GGCGGAGAAA CGAGCAATGT GGAGGGCAGC ACGATCCTCA 
GCTCTTGAAG ACAGTATTAA ACAGTACGAG CAGGACCTGG CCAGGAGGCT GTACCAGTCC 
CGACAGCGGG CACCTGCTCC CGGGGCCGGG CCAGCTCCGA TGCCTAGTCC CAAGCAGAGT 
CACGCCTCGG CACCCCCTGG GTCAGGAAGC CATTCCAGGA TGAAATCGCT TGAACAGGAT 
GCTCTGAAAG CCCAGATGGT CATTGCCAAG TCCAAGGAGG GGAAGAAGCG CAGCCCGCTG 
GAGCAGCTGG CAGAGTCCCC CTCGCCAGTT CCTACACCGT CACCGACACC ACTGGAAGAT 
TGCAGTCCCA GGAACGTCAC CTCCCCAGGA AGGCTGTCCA TGTCTGAAAA GAAGTTTGAC 
TACCGGGAAT TTGCTGCTAT TCCTTCCTCC AAACCCGTTT ACGAAATTCA GTCGCCTGAT 
GCTGCTGATG ACCTCAGATA CATCGAGGAC AGGAATAACT CAGTTCCCCA GGAGCAGGAT 
GGGCAGCCCG AGGAAGAGGA CACATTAGGA ACTCACGACT CATCCACACC CACTCCATCA 
GCACTTGAAG AAATGGCCCT GTACAGCAGC AAACGCAAGC TCCGACACAG CCGGCGCAGC 
CTGGAGGCTG CCGTCCCTAC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCRIB ( XM_014945717.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014801203.1 Calidris pugnax scribbled planar cell polarity protein (SCRIB), transcript variant X4, mRNA.

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CloneID	OCa67750
Clone ID Related Accession (Same CDS sequence)	XM_014945717.1
Accession Version	XM_014945717.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5898bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	protein scribble homolog isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090871.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCCGC TCTGGCGCTG CAACCGGCAC GTGGAGGCGC TGGACCGGCG GCACTGCTCG 
CTGCAGGCCG TGCCCGAGGA GATCTACCGC TACAGCCGCT CGCTGGAGGA GCTGCTGCTG 
GACGCCAACC AGCTGCGGGA GCTGCCCAAG CCTTTCTTCA GGCTTCTGAA CCTGCGCAAG 
CTGGGCCTGA GTGACAATGA GATCCAAAGA CTTCCCCCTG AGGTGGCCAA CTTCATGCAG 
CTGGTGGAAC TGGACATCTC CCGTAACGAC ATTCCAGAAA TTCCTGAAAG CATCAAATTC 
TGCAAATCCT TGGAAATCGC AGACTTCAGT GGGAATCCTC TCTCCAGGCT TCCCGAGGGC 
TTCACGCAGC TTCGGAGCCT GGGCCATTTG GCCCTGAATG ATGTGTCCCT GCAGAGCCTC 
CCCAGTGACA TTGGGAATTT GGCAAACCTG GTGACTCTGG AGCTACGCGA GAACCTGCTA 
AAGACTCTCC CCACCTCGCT GTCCTTCCTT GTCAAGTTAG AGCAGCTGGA TCTCGGTGGC 
AATGACCTGG AGGTTCTGCC AGACACTCTG GGAGCGCTCC CCAACCTGCG TGAGCTCTGG 
TTAGACCGGA ACCAGCTCTC AGCCCTGCCT CCCGAGCTGG GCAACCTCCG CCGCCTGGTC 
TGCCTGGATG TGTCGGAGAA CAAGCTGGAG CAGTTGCCCA ACGAGGTCAG CGGCCTGGTG 
GCACTGACCG ACTTGCTGCT GTCGCAGAAC CTGTTGGAGT GCATTCCTGA TGGGATTGGT 
CAGCTGAAGC AGCTCTCCAT CCTCAAGGTG GACCAGAATC GACTGACAGA GGTGACAGAG 
TCCATTGGGG ACTGTGAAAA TCTGTCCGAG CTCATCCTGA CAGAGAACAT GCTGACGGCC 
TTGCCGAAAT CCCTTGGGAA GCTGGCCAAG CTGACCAACC TGAACGTGGA TCGGAACCGG 
CTGACATCCC TCCCGGCCGA GATCGGTGGC TGTGCCAACC TCAACGTGCT GTCCTTGCGG 
GACAATCGCC TGACCCTCCT GCCATCGGAG CTGGCCAACA CCACCGAGCT TCATGTTCTG 
GATGTGGCTG GGAACAGGCT GCAGAACTTG CCGTTTGCTC TGACAAACCT CAACCTGAAA 
GCCCTGTGGC TGGCGGAGAA TCAGTCCCAG CCCATGCTGA AATTCCAAAC AGAGGATGAT 
GAGAAGACAG GAGAGAAAGT TCTCACTTGT TACCTGCTTC CTCAGCAGCC CTCTCCCAGC 
CTGGAGAACC TTCTACAGAA CAGCGTGGAC GAGAGCTGGA CGGACACCAA TTTAAACAGA 
GTCAGTGTGA TTCAGTTCCT GGATGAACCC AAAGCAGATG ATGAAGAAGA GTCTGGAGCT 
GAGAGAAGGG GCTTGCAGCG CAGAGCAACC CCCCATCCCA GCGAGCTGAA GGTGATGAAG 
AAGGTGATTG AAGTTCGGCG CAGTGAGGCC CACGCTGCAA AGCCCAATGC AGACCTGAAT 
TCTCCTCACT CAGAAGAGAA GAGGCTGAGC GCCACGTCCA ACCGCAGCGA GGACTCCCAC 
CTTTCCAACA GCACTGCCTC TGCTGACTGT AGGGCGGAGG AGCAGCAGGA GGAGGCAGAG 
AGGAGTCGGC CAAACGGGCA AGTGCCTGAG ATGCAGGAGC TGGAGAAGGA GGCAAAGTCC 
AGAGTGGACG AGGAGCACAA GGAAGCTGCT GGCCATGAAG AGGAAGATGT GGAAGTGGAA 
TACAATGAGC CCACTGTACA CTTTGCTGAA GACACGCTAA TACGGAGTGA GGATGAAGAT 
GAAGATGAAG AGCGACCGTT CCCAGTGGAG AAGCAGCGGC TCATCCGCAA GGACACCCCT 
CACTATAAGA AACACTTTAA GATAACTAAA TTGCCCAAGC CAGAGGCGGT GGTGGCCCTG 
CTGCAGGGGC TGAACAGCGA GGGGGTCCCC AAGGAGGAAG AGGAGTTGGG AGGCTGCAAC 
AACAACACAG AGCCTGCAGA CCAGGAGGAG GTGTGGGATG AGGAGGACCG GAAGAATGGA 
GCTTTGTCTG CCCAGCCATC GGTGAAGTTG ACTCTGACTA TCGTACGGCA GACGGGGGGG 
CTGGGCATCA GCATCGCAGG GGGCAAGGGC TCCACCCCCT ATAAGGGCGA TGATGAGGGC 
ATCTTTATTT CTCGGGTGTC AGAAGAAGGT CCTGCGGCTC GTGCAGGGGT TCGTGTTGGG 
GACAAGCTCC TGGAGGTGAA CGGTGTGTCC CTGCACTCCG CTGAGCACCA CGTGGCAGTG 
GAGGCTCTGC GTGGATCGGG AAGTTCCGTC TCCATGACTG TTCTGCGGGA GCGGATGGTG 
GAGCCGGAGA ATGCCATCAC TGTCACCCCG CTGCGCCCCG AGGATGACTA CAGCCCCCGT 
GAGAGGCGAG GTGGCCTGCG CTTCCCGGAG CGACCCGAGG GGACACCTCC CACCGAGAGA 
TACTCCACGT GCCTCATGCG CAACGAGAAG GGACTGGGCT TCAGTATCGC TGGTGGCAAA 
GGCTCCACTC CTTACCGGGC TGGTGACATG GGGATCTTTA TCTCCCGGAT CGCAGAGGGT 
GGTGCAGCCC ATCGGGACGG GATCCTGCAC GTAGGAGATC GGGTCATCTC GATCAATGGG 
GTAGACATGA CAGAAGCCAG GCATGATCAG GCAGTAGCGC TGCTTACCGC GTCCTCCCCC 
ACCATCGTGC TGCTGGTAGA GAGAGAGGGT GCGGAGCAGC CCGGCGAGGG AGACTGGCCC 
GGGGGGCCAC GGGCACGCAT GCACTCCCCA CCGCCACCCC CCAGCCATGG GGAGAGCCCG 
CCCGAGGAGA CGCCCGCACC ACAGAGGAAC CATCTCTCCA AAGGCCTGGA GGATCAGTAC 
CCCATTGAGG AAATTCACCT GGTGAAAGCA GGTGGTCCCC TGGGGCTCAG CATCGTAGGG 
GGCAGTGACC ACTCAAGCCA TCCGTTTGGG ATTCATGAAC CAGGTGTCTT CATTTCGAAG 
GTCATTCCCC GTGGGCTGGC ATCCCGCAGT GGGCTGCGAG TAGGGGACAG GATCCTGGAA 
GTCAATAGCA TTGACCTGCG CCACGCTACC CACCAGGAAG CGGTGAACGC CCTGCTCTCC 
AACACCCAGG AGCTGACCAT GTTAGTGAGG CGAGACCCAC CACCACCTGG TATGCAGGAA 
ATATGCATTG AGAAGGCTCC AGGAGAAAAG CTGGGCATCA GCATCCGGGG TGGAGCGAAA 
GGCCATGCTG GAAATCCGTT TGATCCCACT GACGAGGGCA TCTTCATCTC TAAGGTGAGC 
TCCTCGGGGG CGGCAGCCCG GGACGGCCGC CTGAAGGTGG GGATGCGGAT CCTAGAGGTG 
AACCACCAGA GCCTGCTGGG GATGACGCAC ACGGAAGCGG TGCAGATGCT GCGCGGGGTG 
GGCGACGCGC TCCTGGTGCT GGTGTGTGAC GGCTTCGACC CCAAGGCTGC AGCTGCCATC 
GAGATGTCCC CGGGAATTAT TGCAAACCCT TTCGCTGCTG GTATCGGACG CAAGAATAGC 
CTGGAAAGTA TCTCTTCCAT CGACCGGGAT TTAAGCCCTG AGGAACTGGA GATCCTGCAG 
AAGGAGATGG AAATGGTGAG AGAAGCAACT CAGTGGGAGA GAGAAGAAAT GGAGAAAGTG 
GAGCGGATGC GTAGGGAGCG GGAGGCAGCC ACCCGGCAGC TTGAGGAGGA GGCGGAGAAC 
GTCACCAGCG AGCCCTTGAG ACTGGACTAT CGGACCCTGG CTGCGCTGCC GAGCAGTGGT 
CTCCAGAGAG TCAACCGCAC CCTCGTTCAG TATAATAAGC CCAGCTCCAG AATGGAGGGC 
GACTGTGCTC TGCTGTCTCT GCCGGTGGCA CGGGACAGCC GGGATTCATG CCCTGACACG 
GCTGCTGGCC CCACCATCGG AAGCGTGGCC AGACCTCCCC GGGACTCCGC AACACCCGAC 
CACTGCACCA GCGGGGCAGA AACGCCCCGG CCTGACCAGC AGCCTCCCAC AGGTGGCCCC 
TTGTCCCAGG ACCAGCCGCC AAAGACGCCA GAGCAAGAGT GCATGGTGGA CTCCCAGCCC 
ATCCTCTTCA GTGAGAACCC CTTTGTGGTG GCCAACCGCA GGGGGAAGGC GGCCGGCCGG 
GCCTGCCTGG GGGGGCCTCC CCTGGGCTAT GGCAGGGGGG GAGTGCTGAA GACCAACCTT 
TACTCCAAGG CTCCTGCAGG CGAGTCTGGG ATGGGCAATT CCAGGCGTGA AACCCCCTAT 
TCTCCTGGTA ACCAGCAGCG GCCTGCAACC CCTCGGCCCG CTCACCTGCC GGGCAGGGAG 
ACCCGCTTTG CTTCCATTAA CTTCATTACA TCGCAGGATG ACAGTAAATC GACCAAGCCA 
GGCGTCATTC AGCCTCTTTC CCGAGTGCGA CAAAATGCTG CCCCGCACAA TTCGGAGGAG 
GGGGACAGCC CCAATCCCTT CCAGCAGCCC GAGCCGTGCT TCAGTGTCCA GAACTCTCCA 
AAGCCACCCT CACCACCCTC TCCTGATGAG GTCCCCATCA ATGTCAAGCA AGCGTACAAG 
ACCTTCGCTG CAGTGCCCTT GTCACACCCC CTGCTTGGGA CACAGGAATT GCCTGGTCAG 
AGCGGCACAG AGAATCCCAG AAGTGCCCCG GAGGTGGCTG CGCACAGCCC TGGGGGACTG 
CACAGCCCTG AGCAGAAGTC CTTCCGTGAG AGGCAGAAGT ATTTCGAGAT TGAGGTGAAG 
CAGCAGCAGA TGGACAAGCC CCCCAAGAGA GTCTCCTTGG TGGGAGAGGA CGACCTGAAG 
AAGATGAAAG AGGAGGAAGC TCGAAAACTG CAGCAGAAAC GTACCCTTCT GCTGGAGGAA 
GAGGCAGAAG AGGAGGAGAT GGCGAAGCAG GCGCCTGAGG TGAGCGTGCA GTCCAGTGTC 
ATCATTGAAG GAGTCGAGTA CAAGATTGAG AGACTCAACG GGAGGAGCAG CCAGTCTCCG 
GCCACTCGGC CCTCCGAGGT CAACGAGAAC AGCAGCTCGC AGAGGAATTC ACTGGAAGAG 
GGGATCAAGG CTGAGCAGAG AACCAACTCC ATGTCTGGGC TGAGTCCCTT GTACCTTGGA 
GCAGGGGACG CAGTGGCACC CGTGCGGACG GCCAAAGCTG AACGGCGACA CCAGGAGCGG 
TTGCGAATGC AGAGTCCTGA GCTGCTGAGT GGCCAAGAGA AGGAGCTTTC TCCTGCAGAA 
CGTCGTGCTC TGGAGGCGGA GAAACGAGCA ATGTGGAGGG CAGCACGATC CTCAGCTCTT 
GAAGACAGTA TTAAACAGTA CGAGCAGGAC CTGGCCAGGA GGCTGTACCA GTCCCGACAG 
CGGGCACCTG CTCCCGGGGC CGGGCCAGCT CCGATGCCTA GTCCCAAGCA GAGTCACGCC 
TCGGCACCCC CTGGGTCAGG AAGCCATTCC AGGATGAAAT CGCTTGAACA GGATGCTCTG 
AAAGCCCAGA TGGTCATTGC CAAGTCCAAG GAGGGGAAGA AGCGCAGCCC GCTGGAGCAG 
CTGGCAGAGT CCCCCTCGCC AGTTCCTACA CCGTCACCGA CACCACTGGA AGATTGCAGT 
CCCAGGAACG TCACCTCCCC AGGAAGGCTG TCCATGTCTG AAAAGAAGTT TGACTACCGG 
GAATTTGCTG CTATTCCTTC CTCCAAACCC GTTTACGAAA TTCAGTCGCC TGATGCTGCT 
GATGACCTCA GATACATCGA GGACAGGAAT AACTCAGTTC CCCAGGAGCA GGATGGGCAG 
CCCGAGGAAG AGGACACATT AGGAACTCAC GACTCATCCA CACCCACTCC ATCAGCACTT 
GAAGAAATGG CCCTGTACAG CAGCAAACGC AAGCTCCGAC ACAGCCGGCG CAGCCTGGAG 
GCTGCCGTCC CTACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014801203.1
CDS	6..5903
Translation	MPPLWRCNRHVEALDRRHCSLQAVPEEIYRYSRSLEELLLDANQLRELPKPFFRLLNLRKLGLSDNEIQRLPPEVANFMQLVELDISRNDIPEIPESIKFCKSLEIADFSGNPLSRLPEGFTQLRSLGHLALNDVSLQSLPSDIGNLANLVTLELRENLLKTLPTSLSFLVKLEQLDLGGNDLEVLPDTLGALPNLRELWLDRNQLSALPPELGNLRRLVCLDVSENKLEQLPNEVSGLVALTDLLLSQNLLECIPDGIGQLKQLSILKVDQNRLTEVTESIGDCENLSELILTENMLTALPKSLGKLAKLTNLNVDRNRLTSLPAEIGGCANLNVLSLRDNRLTLLPSELANTTELHVLDVAGNRLQNLPFALTNLNLKALWLAENQSQPMLKFQTEDDEKTGEKVLTCYLLPQQPSPSLENLLQNSVDESWTDTNLNRVSVIQFLDEPKADDEEESGAERRGLQRRATPHPSELKVMKKVIEVRRSEAHAAKPNADLNSPHSEEKRLSATSNRSEDSHLSNSTASADCRAEEQQEEAERSRPNGQVPEMQELEKEAKSRVDEEHKEAAGHEEEDVEVEYNEPTVHFAEDTLIRSEDEDEDEERPFPVEKQRLIRKDTPHYKKHFKITKLPKPEAVVALLQGLNSEGVPKEEEELGGCNNNTEPADQEEVWDEEDRKNGALSAQPSVKLTLTIVRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVRVGDKLLEVNGVSLHSAEHHVAVEALRGSGSSVSMTVLRERMVEPENAITVTPLRPEDDYSPRERRGGLRFPERPEGTPPTERYSTCLMRNEKGLGFSIAGGKGSTPYRAGDMGIFISRIAEGGAAHRDGILHVGDRVISINGVDMTEARHDQAVALLTASSPTIVLLVEREGAEQPGEGDWPGGPRARMHSPPPPPSHGESPPEETPAPQRNHLSKGLEDQYPIEEIHLVKAGGPLGLSIVGGSDHSSHPFGIHEPGVFISKVIPRGLASRSGLRVGDRILEVNSIDLRHATHQEAVNALLSNTQELTMLVRRDPPPPGMQEICIEKAPGEKLGISIRGGAKGHAGNPFDPTDEGIFISKVSSSGAAARDGRLKVGMRILEVNHQSLLGMTHTEAVQMLRGVGDALLVLVCDGFDPKAAAAIEMSPGIIANPFAAGIGRKNSLESISSIDRDLSPEELEILQKEMEMVREATQWEREEMEKVERMRREREAATRQLEEEAENVTSEPLRLDYRTLAALPSSGLQRVNRTLVQYNKPSSRMEGDCALLSLPVARDSRDSCPDTAAGPTIGSVARPPRDSATPDHCTSGAETPRPDQQPPTGGPLSQDQPPKTPEQECMVDSQPILFSENPFVVANRRGKAAGRACLGGPPLGYGRGGVLKTNLYSKAPAGESGMGNSRRETPYSPGNQQRPATPRPAHLPGRETRFASINFITSQDDSKSTKPGVIQPLSRVRQNAAPHNSEEGDSPNPFQQPEPCFSVQNSPKPPSPPSPDEVPINVKQAYKTFAAVPLSHPLLGTQELPGQSGTENPRSAPEVAAHSPGGLHSPEQKSFRERQKYFEIEVKQQQMDKPPKRVSLVGEDDLKKMKEEEARKLQQKRTLLLEEEAEEEEMAKQAPEVSVQSSVIIEGVEYKIERLNGRSSQSPATRPSEVNENSSSQRNSLEEGIKAEQRTNSMSGLSPLYLGAGDAVAPVRTAKAERRHQERLRMQSPELLSGQEKELSPAERRALEAEKRAMWRAARSSALEDSIKQYEQDLARRLYQSRQRAPAPGAGPAPMPSPKQSHASAPPGSGSHSRMKSLEQDALKAQMVIAKSKEGKKRSPLEQLAESPSPVPTPSPTPLEDCSPRNVTSPGRLSMSEKKFDYREFAAIPSSKPVYEIQSPDAADDLRYIEDRNNSVPQEQDGQPEEEDTLGTHDSSTPTPSALEEMALYSSKRKLRHSRRSLEAAVPT

Target ORF information:

RefSeq Version	XM_014945717.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax scribbled planar cell polarity protein (SCRIB), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014945717.1

ORF Insert Sequence:

ATGCCGCCGC TCTGGCGCTG CAACCGGCAC GTGGAGGCGC TGGACCGGCG GCACTGCTCG 
CTGCAGGCCG TGCCCGAGGA GATCTACCGC TACAGCCGCT CGCTGGAGGA GCTGCTGCTG 
GACGCCAACC AGCTGCGGGA GCTGCCCAAG CCTTTCTTCA GGCTTCTGAA CCTGCGCAAG 
CTGGGCCTGA GTGACAATGA GATCCAAAGA CTTCCCCCTG AGGTGGCCAA CTTCATGCAG 
CTGGTGGAAC TGGACATCTC CCGTAACGAC ATTCCAGAAA TTCCTGAAAG CATCAAATTC 
TGCAAATCCT TGGAAATCGC AGACTTCAGT GGGAATCCTC TCTCCAGGCT TCCCGAGGGC 
TTCACGCAGC TTCGGAGCCT GGGCCATTTG GCCCTGAATG ATGTGTCCCT GCAGAGCCTC 
CCCAGTGACA TTGGGAATTT GGCAAACCTG GTGACTCTGG AGCTACGCGA GAACCTGCTA 
AAGACTCTCC CCACCTCGCT GTCCTTCCTT GTCAAGTTAG AGCAGCTGGA TCTCGGTGGC 
AATGACCTGG AGGTTCTGCC AGACACTCTG GGAGCGCTCC CCAACCTGCG TGAGCTCTGG 
TTAGACCGGA ACCAGCTCTC AGCCCTGCCT CCCGAGCTGG GCAACCTCCG CCGCCTGGTC 
TGCCTGGATG TGTCGGAGAA CAAGCTGGAG CAGTTGCCCA ACGAGGTCAG CGGCCTGGTG 
GCACTGACCG ACTTGCTGCT GTCGCAGAAC CTGTTGGAGT GCATTCCTGA TGGGATTGGT 
CAGCTGAAGC AGCTCTCCAT CCTCAAGGTG GACCAGAATC GACTGACAGA GGTGACAGAG 
TCCATTGGGG ACTGTGAAAA TCTGTCCGAG CTCATCCTGA CAGAGAACAT GCTGACGGCC 
TTGCCGAAAT CCCTTGGGAA GCTGGCCAAG CTGACCAACC TGAACGTGGA TCGGAACCGG 
CTGACATCCC TCCCGGCCGA GATCGGTGGC TGTGCCAACC TCAACGTGCT GTCCTTGCGG 
GACAATCGCC TGACCCTCCT GCCATCGGAG CTGGCCAACA CCACCGAGCT TCATGTTCTG 
GATGTGGCTG GGAACAGGCT GCAGAACTTG CCGTTTGCTC TGACAAACCT CAACCTGAAA 
GCCCTGTGGC TGGCGGAGAA TCAGTCCCAG CCCATGCTGA AATTCCAAAC AGAGGATGAT 
GAGAAGACAG GAGAGAAAGT TCTCACTTGT TACCTGCTTC CTCAGCAGCC CTCTCCCAGC 
CTGGAGAACC TTCTACAGAA CAGCGTGGAC GAGAGCTGGA CGGACACCAA TTTAAACAGA 
GTCAGTGTGA TTCAGTTCCT GGATGAACCC AAAGCAGATG ATGAAGAAGA GTCTGGAGCT 
GAGAGAAGGG GCTTGCAGCG CAGAGCAACC CCCCATCCCA GCGAGCTGAA GGTGATGAAG 
AAGGTGATTG AAGTTCGGCG CAGTGAGGCC CACGCTGCAA AGCCCAATGC AGACCTGAAT 
TCTCCTCACT CAGAAGAGAA GAGGCTGAGC GCCACGTCCA ACCGCAGCGA GGACTCCCAC 
CTTTCCAACA GCACTGCCTC TGCTGACTGT AGGGCGGAGG AGCAGCAGGA GGAGGCAGAG 
AGGAGTCGGC CAAACGGGCA AGTGCCTGAG ATGCAGGAGC TGGAGAAGGA GGCAAAGTCC 
AGAGTGGACG AGGAGCACAA GGAAGCTGCT GGCCATGAAG AGGAAGATGT GGAAGTGGAA 
TACAATGAGC CCACTGTACA CTTTGCTGAA GACACGCTAA TACGGAGTGA GGATGAAGAT 
GAAGATGAAG AGCGACCGTT CCCAGTGGAG AAGCAGCGGC TCATCCGCAA GGACACCCCT 
CACTATAAGA AACACTTTAA GATAACTAAA TTGCCCAAGC CAGAGGCGGT GGTGGCCCTG 
CTGCAGGGGC TGAACAGCGA GGGGGTCCCC AAGGAGGAAG AGGAGTTGGG AGGCTGCAAC 
AACAACACAG AGCCTGCAGA CCAGGAGGAG GTGTGGGATG AGGAGGACCG GAAGAATGGA 
GCTTTGTCTG CCCAGCCATC GGTGAAGTTG ACTCTGACTA TCGTACGGCA GACGGGGGGG 
CTGGGCATCA GCATCGCAGG GGGCAAGGGC TCCACCCCCT ATAAGGGCGA TGATGAGGGC 
ATCTTTATTT CTCGGGTGTC AGAAGAAGGT CCTGCGGCTC GTGCAGGGGT TCGTGTTGGG 
GACAAGCTCC TGGAGGTGAA CGGTGTGTCC CTGCACTCCG CTGAGCACCA CGTGGCAGTG 
GAGGCTCTGC GTGGATCGGG AAGTTCCGTC TCCATGACTG TTCTGCGGGA GCGGATGGTG 
GAGCCGGAGA ATGCCATCAC TGTCACCCCG CTGCGCCCCG AGGATGACTA CAGCCCCCGT 
GAGAGGCGAG GTGGCCTGCG CTTCCCGGAG CGACCCGAGG GGACACCTCC CACCGAGAGA 
TACTCCACGT GCCTCATGCG CAACGAGAAG GGACTGGGCT TCAGTATCGC TGGTGGCAAA 
GGCTCCACTC CTTACCGGGC TGGTGACATG GGGATCTTTA TCTCCCGGAT CGCAGAGGGT 
GGTGCAGCCC ATCGGGACGG GATCCTGCAC GTAGGAGATC GGGTCATCTC GATCAATGGG 
GTAGACATGA CAGAAGCCAG GCATGATCAG GCAGTAGCGC TGCTTACCGC GTCCTCCCCC 
ACCATCGTGC TGCTGGTAGA GAGAGAGGGT GCGGAGCAGC CCGGCGAGGG AGACTGGCCC 
GGGGGGCCAC GGGCACGCAT GCACTCCCCA CCGCCACCCC CCAGCCATGG GGAGAGCCCG 
CCCGAGGAGA CGCCCGCACC ACAGAGGAAC CATCTCTCCA AAGGCCTGGA GGATCAGTAC 
CCCATTGAGG AAATTCACCT GGTGAAAGCA GGTGGTCCCC TGGGGCTCAG CATCGTAGGG 
GGCAGTGACC ACTCAAGCCA TCCGTTTGGG ATTCATGAAC CAGGTGTCTT CATTTCGAAG 
GTCATTCCCC GTGGGCTGGC ATCCCGCAGT GGGCTGCGAG TAGGGGACAG GATCCTGGAA 
GTCAATAGCA TTGACCTGCG CCACGCTACC CACCAGGAAG CGGTGAACGC CCTGCTCTCC 
AACACCCAGG AGCTGACCAT GTTAGTGAGG CGAGACCCAC CACCACCTGG TATGCAGGAA 
ATATGCATTG AGAAGGCTCC AGGAGAAAAG CTGGGCATCA GCATCCGGGG TGGAGCGAAA 
GGCCATGCTG GAAATCCGTT TGATCCCACT GACGAGGGCA TCTTCATCTC TAAGGTGAGC 
TCCTCGGGGG CGGCAGCCCG GGACGGCCGC CTGAAGGTGG GGATGCGGAT CCTAGAGGTG 
AACCACCAGA GCCTGCTGGG GATGACGCAC ACGGAAGCGG TGCAGATGCT GCGCGGGGTG 
GGCGACGCGC TCCTGGTGCT GGTGTGTGAC GGCTTCGACC CCAAGGCTGC AGCTGCCATC 
GAGATGTCCC CGGGAATTAT TGCAAACCCT TTCGCTGCTG GTATCGGACG CAAGAATAGC 
CTGGAAAGTA TCTCTTCCAT CGACCGGGAT TTAAGCCCTG AGGAACTGGA GATCCTGCAG 
AAGGAGATGG AAATGGTGAG AGAAGCAACT CAGTGGGAGA GAGAAGAAAT GGAGAAAGTG 
GAGCGGATGC GTAGGGAGCG GGAGGCAGCC ACCCGGCAGC TTGAGGAGGA GGCGGAGAAC 
GTCACCAGCG AGCCCTTGAG ACTGGACTAT CGGACCCTGG CTGCGCTGCC GAGCAGTGGT 
CTCCAGAGAG TCAACCGCAC CCTCGTTCAG TATAATAAGC CCAGCTCCAG AATGGAGGGC 
GACTGTGCTC TGCTGTCTCT GCCGGTGGCA CGGGACAGCC GGGATTCATG CCCTGACACG 
GCTGCTGGCC CCACCATCGG AAGCGTGGCC AGACCTCCCC GGGACTCCGC AACACCCGAC 
CACTGCACCA GCGGGGCAGA AACGCCCCGG CCTGACCAGC AGCCTCCCAC AGGTGGCCCC 
TTGTCCCAGG ACCAGCCGCC AAAGACGCCA GAGCAAGAGT GCATGGTGGA CTCCCAGCCC 
ATCCTCTTCA GTGAGAACCC CTTTGTGGTG GCCAACCGCA GGGGGAAGGC GGCCGGCCGG 
GCCTGCCTGG GGGGGCCTCC CCTGGGCTAT GGCAGGGGGG GAGTGCTGAA GACCAACCTT 
TACTCCAAGG CTCCTGCAGG CGAGTCTGGG ATGGGCAATT CCAGGCGTGA AACCCCCTAT 
TCTCCTGGTA ACCAGCAGCG GCCTGCAACC CCTCGGCCCG CTCACCTGCC GGGCAGGGAG 
ACCCGCTTTG CTTCCATTAA CTTCATTACA TCGCAGGATG ACAGTAAATC GACCAAGCCA 
GGCGTCATTC AGCCTCTTTC CCGAGTGCGA CAAAATGCTG CCCCGCACAA TTCGGAGGAG 
GGGGACAGCC CCAATCCCTT CCAGCAGCCC GAGCCGTGCT TCAGTGTCCA GAACTCTCCA 
AAGCCACCCT CACCACCCTC TCCTGATGAG GTCCCCATCA ATGTCAAGCA AGCGTACAAG 
ACCTTCGCTG CAGTGCCCTT GTCACACCCC CTGCTTGGGA CACAGGAATT GCCTGGTCAG 
AGCGGCACAG AGAATCCCAG AAGTGCCCCG GAGGTGGCTG CGCACAGCCC TGGGGGACTG 
CACAGCCCTG AGCAGAAGTC CTTCCGTGAG AGGCAGAAGT ATTTCGAGAT TGAGGTGAAG 
CAGCAGCAGA TGGACAAGCC CCCCAAGAGA GTCTCCTTGG TGGGAGAGGA CGACCTGAAG 
AAGATGAAAG AGGAGGAAGC TCGAAAACTG CAGCAGAAAC GTACCCTTCT GCTGGAGGAA 
GAGGCAGAAG AGGAGGAGAT GGCGAAGCAG GCGCCTGAGG TGAGCGTGCA GTCCAGTGTC 
ATCATTGAAG GAGTCGAGTA CAAGATTGAG AGACTCAACG GGAGGAGCAG CCAGTCTCCG 
GCCACTCGGC CCTCCGAGGT CAACGAGAAC AGCAGCTCGC AGAGGAATTC ACTGGAAGAG 
GGGATCAAGG CTGAGCAGAG AACCAACTCC ATGTCTGGGC TGAGTCCCTT GTACCTTGGA 
GCAGGGGACG CAGTGGCACC CGTGCGGACG GCCAAAGCTG AACGGCGACA CCAGGAGCGG 
TTGCGAATGC AGAGTCCTGA GCTGCTGAGT GGCCAAGAGA AGGAGCTTTC TCCTGCAGAA 
CGTCGTGCTC TGGAGGCGGA GAAACGAGCA ATGTGGAGGG CAGCACGATC CTCAGCTCTT 
GAAGACAGTA TTAAACAGTA CGAGCAGGAC CTGGCCAGGA GGCTGTACCA GTCCCGACAG 
CGGGCACCTG CTCCCGGGGC CGGGCCAGCT CCGATGCCTA GTCCCAAGCA GAGTCACGCC 
TCGGCACCCC CTGGGTCAGG AAGCCATTCC AGGATGAAAT CGCTTGAACA GGATGCTCTG 
AAAGCCCAGA TGGTCATTGC CAAGTCCAAG GAGGGGAAGA AGCGCAGCCC GCTGGAGCAG 
CTGGCAGAGT CCCCCTCGCC AGTTCCTACA CCGTCACCGA CACCACTGGA AGATTGCAGT 
CCCAGGAACG TCACCTCCCC AGGAAGGCTG TCCATGTCTG AAAAGAAGTT TGACTACCGG 
GAATTTGCTG CTATTCCTTC CTCCAAACCC GTTTACGAAA TTCAGTCGCC TGATGCTGCT 
GATGACCTCA GATACATCGA GGACAGGAAT AACTCAGTTC CCCAGGAGCA GGATGGGCAG 
CCCGAGGAAG AGGACACATT AGGAACTCAC GACTCATCCA CACCCACTCC ATCAGCACTT 
GAAGAAATGG CCCTGTACAG CAGCAAACGC AAGCTCCGAC ACAGCCGGCG CAGCCTGGAG 
GCTGCCGTCC CTACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCRIB ( XM_014945718.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014801204.1 Calidris pugnax scribbled planar cell polarity protein (SCRIB), transcript variant X5, mRNA.

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CloneID	OCa67751
Clone ID Related Accession (Same CDS sequence)	XM_014945718.1
Accession Version	XM_014945718.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5886bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	protein scribble homolog isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090871.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCCGC TCTGGCGCTG CAACCGGCAC GTGGAGGCGC TGGACCGGCG GCACTGCTCG 
CTGCAGGCCG TGCCCGAGGA GATCTACCGC TACAGCCGCT CGCTGGAGGA GCTGCTGCTG 
GACGCCAACC AGCTGCGGGA GCTGCCCAAG CCTTTCTTCA GGCTTCTGAA CCTGCGCAAG 
CTGGGCCTGA GTGACAATGA GATCCAAAGA CTTCCCCCTG AGGTGGCCAA CTTCATGCAG 
CTGGTGGAAC TGGACATCTC CCGTAACGAC ATTCCAGAAA TTCCTGAAAG CATCAAATTC 
TGCAAATCCT TGGAAATCGC AGACTTCAGT GGGAATCCTC TCTCCAGGCT TCCCGAGGGC 
TTCACGCAGC TTCGGAGCCT GGGCCATTTG GCCCTGAATG ATGTGTCCCT GCAGAGCCTC 
CCCAGTGACA TTGGGAATTT GGCAAACCTG GTGACTCTGG AGCTACGCGA GAACCTGCTA 
AAGACTCTCC CCACCTCGCT GTCCTTCCTT GTCAAGTTAG AGCAGCTGGA TCTCGGTGGC 
AATGACCTGG AGGTTCTGCC AGACACTCTG GGAGCGCTCC CCAACCTGCG TGAGCTCTGG 
TTAGACCGGA ACCAGCTCTC AGCCCTGCCT CCCGAGCTGG GCAACCTCCG CCGCCTGGTC 
TGCCTGGATG TGTCGGAGAA CAAGCTGGAG CAGTTGCCCA ACGAGGTCAG CGGCCTGGTG 
GCACTGACCG ACTTGCTGCT GTCGCAGAAC CTGTTGGAGT GCATTCCTGA TGGGATTGGT 
CAGCTGAAGC AGCTCTCCAT CCTCAAGGTG GACCAGAATC GACTGACAGA GGTGACAGAG 
TCCATTGGGG ACTGTGAAAA TCTGTCCGAG CTCATCCTGA CAGAGAACAT GCTGACGGCC 
TTGCCGAAAT CCCTTGGGAA GCTGGCCAAG CTGACCAACC TGAACGTGGA TCGGAACCGG 
CTGACATCCC TCCCGGCCGA GATCGGTGGC TGTGCCAACC TCAACGTGCT GTCCTTGCGG 
GACAATCGCC TGACCCTCCT GCCATCGGAG CTGGCCAACA CCACCGAGCT TCATGTTCTG 
GATGTGGCTG GGAACAGGCT GCAGAACTTG CCGTTTGCTC TGACAAACCT CAACCTGAAA 
GCCCTGTGGC TGGCGGAGAA TCAGTCCCAG CCCATGCTGA AATTCCAAAC AGAGGATGAT 
GAGAAGACAG GAGAGAAAGT TCTCACTTGT TACCTGCTTC CTCAGCAGCC CTCTCCCAGC 
CTGGAGAACC TTCTACAGAA CAGCGTGGAC GAGAGCTGGA CGGACACCAA TTTAAACAGA 
GTCAGTGTGA TTCAGTTCCT GGATGAACCC AAAGCAGATG ATGAAGAAGA GTCTGGAGCT 
GAGAGAAGGG GCTTGCAGCG CAGAGCAACC CCCCATCCCA GCGAGCTGAA GGTGATGAAG 
AAGGTGATTG AAGTTCGGCG CAGTGAGGCC CACGCTGCAA AGCCCAATGC AGACCTGAAT 
TCTCCTCACT CAGAAGAGAA GAGGCTGAGC GCCACGTCCA ACCGCAGCGA GGACTCCCAC 
CTTTCCAACA GCACTGCCTC TGCTGACTGT AGGGCGGAGG AGCAGCAGGA GGAGGCAGAG 
AGGAGTCGGC CAAACGGGCA AGTGCCTGAG ATGCAGGAGC TGGAGAAGGA GGCAAAGTCC 
AGAGTGGACG AGGAGCACAA GGAAGCTGCT GGCCATGAAG AGGAAGATGT GGAAGTGGAA 
TACAATGAGC CCACTGTACA CTTTGCTGAA GACACGCTAA TACGGAGTGA GGATGAAGAT 
GAAGATGAAG AGCGACCGTT CCCAGTGGAG AAGCAGCGGC TCATCCGCAA GGACACCCCT 
CACTATAAGA AACACTTTAA GATAACTAAA TTGCCCAAGC CAGAGGCGGT GGTGGCCCTG 
CTGCAGGGGC TGAACAGCGA GGGGGTCCCC AAGGAGGAAG AGGAGTTGGG AGGCTGCAAC 
AACAACACAG AGCCTGCAGA CCAGGAGGAG GTGTGGGATG AGGAGGACCG GAAGAATGGA 
GCTTTGTCTG CCCAGCCATC GGTGAAGGGG GTGTCGTTTG ATCAAGCCAA TAATCTGCTG 
ATTGAACCTG CTCGCATTGA GGAGGAAGAG TTGACTCTGA CTATCGTACG GCAGACGGGG 
GGGCTGGGCA TCAGCATCGC AGGGGGCAAG GGCTCCACCC CCTATAAGGG CGATGATGAG 
GGCATCTTTA TTTCTCGGGT GTCAGAAGAA GGTCCTGCGG CTCGTGCAGG GGTTCGTGTT 
GGGGACAAGC TCCTGGAGGT GAACGGTGTG TCCCTGCACT CCGCTGAGCA CCACGTGGCA 
GTGGAGGCTC TGCGTGGATC GGGAAGTTCC GTCTCCATGA CTGTTCTGCG GGAGCGGATG 
GTGGAGCCGG AGAATGCCAT CACTGTCACC CCGCTGCGCC CCGAGGATGA CTACAGCCCC 
CGTGAGAGGC GAGGTGGCCT GCGCTTCCCG GAGCGACCCG AGGGGACACC TCCCACCGAG 
AGATACTCCA CGTGCCTCAT GCGCAACGAG AAGGGACTGG GCTTCAGTAT CGCTGGTGGC 
AAAGGCTCCA CTCCTTACCG GGCTGGTGAC ATGGGGATCT TTATCTCCCG GATCGCAGAG 
GGTGGTGCAG CCCATCGGGA CGGGATCCTG CACGTAGGAG ATCGGGTCAT CTCGATCAAT 
GGGGTAGACA TGACAGAAGC CAGGCATGAT CAGGCAGTAG CGCTGCTTAC CGCGTCCTCC 
CCCACCATCG TGCTGCTGGT AGAGAGAGAG GGTGCGGAGC AGCCCGGCGA GGGAGACTGG 
CCCGGGGGGC CACGGGCACG CATGCACTCC CCACCGCCAC CCCCCAGCCA TGGGGAGAGC 
CCGCCCGAGG AGACGCCCGC ACCACAGAGG AACCATCTCT CCAAAGGCCT GGAGGATCAG 
TACCCCATTG AGGAAATTCA CCTGGTGAAA GCAGGTGGTC CCCTGGGGCT CAGCATCGTA 
GGGGGCAGTG ACCACTCAAG CCATCCGTTT GGGATTCATG AACCAGGTGT CTTCATTTCG 
AAGGTCATTC CCCGTGGGCT GGCATCCCGC AGTGGGCTGC GAGTAGGGGA CAGGATCCTG 
GAAGTCAATA GCATTGACCT GCGCCACGCT ACCCACCAGG AAGCGGTGAA CGCCCTGCTC 
TCCAACACCC AGGAGCTGAC CATGTTAGTG AGGCGAGACC CACCACCACC TGGTATGCAG 
GAAATATGCA TTGAGAAGGC TCCAGGAGAA AAGCTGGGCA TCAGCATCCG GGGTGGAGCG 
AAAGGCCATG CTGGAAATCC GTTTGATCCC ACTGACGAGG GCATCTTCAT CTCTAAGGTG 
AGCTCCTCGG GGGCGGCAGC CCGGGACGGC CGCCTGAAGG TGGGGATGCG GATCCTAGAG 
GTGAACCACC AGAGCCTGCT GGGGATGACG CACACGGAAG CGGTGCAGAT GCTGCGCGGG 
GTGGGCGACG CGCTCCTGGT GCTGGTGTGT GACGGCTTCG ACCCCAAGGC TGCAGCTGCC 
ATCGAGATGT CCCCGGGAAT TATTGCAAAC CCTTTCGCTG CTGGTATCGG ACGCAAGAAT 
AGCCTGGAAA GTATCTCTTC CATCGACCGG GATTTAAGCC CTGAGGAACT GGAGATCCTG 
CAGAAGGAGA TGGAAATGGT GAGAGAAGCA ACTCAGTGGG AGAGAGAAGA AATGGAGAAA 
GTGGAGCGGA TGCGTAGGGA GCGGGAGGCA GCCACCCGGC AGCTTGAGGA GGAGGCGGAG 
AACGTCACCA GCGAGCCCTT GAGACTGGAC TATCGGACCC TGGCTGCGCT GCCGAGCAGT 
GGTCTCCAGA GAGTCAACCG CACCCTCGTT CAGTATAATA AGCCCAGCTC CAGAATGGAG 
GGCGACTGTG CTCTGCTGTC TCTGCCGGTG GCACGGGACA GCCGGGATTC ATGCCCTGAC 
ACGGCTGCTG GCCCCACCAT CGGAAGCGTG GCCAGACCTC CCCGGGACTC CGCAACACCC 
GACCACTGCA CCAGCGGGGC AGAAACGCCC CGGCCTGACC AGCAGCCTCC CACAGGTGGC 
CCCTTGTCCC AGGACCAGCC GCCAAAGACG CCAGAGCAAG AGTGCATGGT GGACTCCCAG 
CCCATCCTCT TCAGTGAGAA CCCCTTTGTG GTGGCCAACC GCAGGGGGAA GGCGGCCGGC 
CGGGCCTGCC TGGGGGGGCC TCCCCTGGGC TATGGCAGGG GGGGAGTGCT GAAGACCAAC 
CTTTACTCCA AGGCTCCTGC AGGCGAGTCT GGGATGGGCA ATTCCAGGCG TGAAACCCCC 
TATTCTCCTG GTAACCAGCA GCGGCCTGCA ACCCCTCGGC CCGCTCACCT GCCGGGCAGG 
GAGACCCGCT TTGCTTCCAT TAACTTCATT ACATCGCAGG ATGACAGTAA ATCGACCAAG 
CCAGGCGTCA TTCAGCCTCT TTCCCGAGTG CGACAAAATG CTGCCCCGCA CAATTCGGAG 
GAGGGGGACA GCCCCAATCC CTTCCAGCAG CCCGAGCCGT GCTTCAGTGT CCAGAACTCT 
CCAAAGCCAC CCTCACCACC CTCTCCTGAT GAGGTCCCCA TCAATGTCAA GCAAGCGTAC 
AAGACCTTCG CTGCAGTGCC CTTGTCACAC CCCCTGCTTG GGACACAGGA ATTGCCTGGT 
CAGAGCGGCA CAGAGAATCC CAGAAGTGCC CCGGAGGTGG CTGCGCACAG CCCTGGGGGA 
CTGCACAGCC CTGAGCAGAA GTCCTTCCGT GAGAGGCAGA AGTATTTCGA GATTGAGGTG 
AAGCAGCAGC AGATGGACAA GCCCCCCAAG AGAGTCTCCT TGGTGGGAGA GGACGACCTG 
AAGAAGATGA AAGAGGAGGA AGCTCGAAAA CTGCAGCAGA AACGTACCCT TCTGCTGGAG 
GAAGAGGCAG AAGAGGAGGA GATGGCGAAG CAGGCGCCTG AGGTGAGCGT GCAGTCCAGT 
GTCATCATTG AAGGAGTCGA GTACAAGATT GAGAGACTCA ACGGGAGGAG CAGCCAGTCT 
CCGGCCACTC GGCCCTCCGA GGTCAACGAG AACAGCAGCT CGCAGAGGAA TTCACTGGAA 
GAGGGGATCA AGGCTGAGCA GAGAACCAAC TCCATGTCTG GGCTGAGTCC CTTGTACCTT 
GGAGCAGGGG ACGCAGTGGC ACCCGTGCGG ACGGCCAAAG CTGAACGGCG ACACCAGGAG 
CGGTTGCGAA TGCAGAGTCC TGAGCTGCTG AGTGGCCAAG AGAAGGAGCT TTCTCCTGCA 
GAACGTCGTG CTCTGGAGGC GGAGAAACGA GCAATGTGGA GGGCAGCACG ATCCTCAGCT 
CTTGAAGACA GTATTAAACA GTACGAGCAG GACCTGGCCA GGAGGCTGTA CCAGTCCCGA 
CAGCGGGCAC CTGCTCCCGG GGCCGGGCCA GCTCCGATGC CTAGTCCCAA GCAGAGTCAC 
GCCTCGGCAC CCCCTGGGTC AGGAAGCCAT TCCAGGATGA AATCGCTTGA ACAGGATGCT 
CTGAAAGCCC AGATGGTCAT TGCCAAGTCC AAGGAGGGGA AGAAGCGCAG CCCGCTGGAG 
CAGCTGGCAG AGTCCCCCTC GCCAGTTCCT ACACCGTCAC CGACACCACT GGAAGATTGC 
AGTCCCAGGA ACGTCACCTC CCCAGGAAGG CTGTCGCCTG ATGCTGCTGA TGACCTCAGA 
TACATCGAGG ACAGGAATAA CTCAGTTCCC CAGGAGCAGG ATGGGCAGCC CGAGGAAGAG 
GACACATTAG GAACTCACGA CTCATCCACA CCCACTCCAT CAGCACTTGA AGAAATGGCC 
CTGTACAGCA GCAAACGCAA GCTCCGACAC AGCCGGCGCA GCCTGGAGGC TGCCGTCCCT 
ACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014801204.1
CDS	6..5891
Translation	MPPLWRCNRHVEALDRRHCSLQAVPEEIYRYSRSLEELLLDANQLRELPKPFFRLLNLRKLGLSDNEIQRLPPEVANFMQLVELDISRNDIPEIPESIKFCKSLEIADFSGNPLSRLPEGFTQLRSLGHLALNDVSLQSLPSDIGNLANLVTLELRENLLKTLPTSLSFLVKLEQLDLGGNDLEVLPDTLGALPNLRELWLDRNQLSALPPELGNLRRLVCLDVSENKLEQLPNEVSGLVALTDLLLSQNLLECIPDGIGQLKQLSILKVDQNRLTEVTESIGDCENLSELILTENMLTALPKSLGKLAKLTNLNVDRNRLTSLPAEIGGCANLNVLSLRDNRLTLLPSELANTTELHVLDVAGNRLQNLPFALTNLNLKALWLAENQSQPMLKFQTEDDEKTGEKVLTCYLLPQQPSPSLENLLQNSVDESWTDTNLNRVSVIQFLDEPKADDEEESGAERRGLQRRATPHPSELKVMKKVIEVRRSEAHAAKPNADLNSPHSEEKRLSATSNRSEDSHLSNSTASADCRAEEQQEEAERSRPNGQVPEMQELEKEAKSRVDEEHKEAAGHEEEDVEVEYNEPTVHFAEDTLIRSEDEDEDEERPFPVEKQRLIRKDTPHYKKHFKITKLPKPEAVVALLQGLNSEGVPKEEEELGGCNNNTEPADQEEVWDEEDRKNGALSAQPSVKGVSFDQANNLLIEPARIEEEELTLTIVRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVRVGDKLLEVNGVSLHSAEHHVAVEALRGSGSSVSMTVLRERMVEPENAITVTPLRPEDDYSPRERRGGLRFPERPEGTPPTERYSTCLMRNEKGLGFSIAGGKGSTPYRAGDMGIFISRIAEGGAAHRDGILHVGDRVISINGVDMTEARHDQAVALLTASSPTIVLLVEREGAEQPGEGDWPGGPRARMHSPPPPPSHGESPPEETPAPQRNHLSKGLEDQYPIEEIHLVKAGGPLGLSIVGGSDHSSHPFGIHEPGVFISKVIPRGLASRSGLRVGDRILEVNSIDLRHATHQEAVNALLSNTQELTMLVRRDPPPPGMQEICIEKAPGEKLGISIRGGAKGHAGNPFDPTDEGIFISKVSSSGAAARDGRLKVGMRILEVNHQSLLGMTHTEAVQMLRGVGDALLVLVCDGFDPKAAAAIEMSPGIIANPFAAGIGRKNSLESISSIDRDLSPEELEILQKEMEMVREATQWEREEMEKVERMRREREAATRQLEEEAENVTSEPLRLDYRTLAALPSSGLQRVNRTLVQYNKPSSRMEGDCALLSLPVARDSRDSCPDTAAGPTIGSVARPPRDSATPDHCTSGAETPRPDQQPPTGGPLSQDQPPKTPEQECMVDSQPILFSENPFVVANRRGKAAGRACLGGPPLGYGRGGVLKTNLYSKAPAGESGMGNSRRETPYSPGNQQRPATPRPAHLPGRETRFASINFITSQDDSKSTKPGVIQPLSRVRQNAAPHNSEEGDSPNPFQQPEPCFSVQNSPKPPSPPSPDEVPINVKQAYKTFAAVPLSHPLLGTQELPGQSGTENPRSAPEVAAHSPGGLHSPEQKSFRERQKYFEIEVKQQQMDKPPKRVSLVGEDDLKKMKEEEARKLQQKRTLLLEEEAEEEEMAKQAPEVSVQSSVIIEGVEYKIERLNGRSSQSPATRPSEVNENSSSQRNSLEEGIKAEQRTNSMSGLSPLYLGAGDAVAPVRTAKAERRHQERLRMQSPELLSGQEKELSPAERRALEAEKRAMWRAARSSALEDSIKQYEQDLARRLYQSRQRAPAPGAGPAPMPSPKQSHASAPPGSGSHSRMKSLEQDALKAQMVIAKSKEGKKRSPLEQLAESPSPVPTPSPTPLEDCSPRNVTSPGRLSPDAADDLRYIEDRNNSVPQEQDGQPEEEDTLGTHDSSTPTPSALEEMALYSSKRKLRHSRRSLEAAVPT

Target ORF information:

RefSeq Version	XM_014945718.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax scribbled planar cell polarity protein (SCRIB), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014945718.1

ORF Insert Sequence:

ATGCCGCCGC TCTGGCGCTG CAACCGGCAC GTGGAGGCGC TGGACCGGCG GCACTGCTCG 
CTGCAGGCCG TGCCCGAGGA GATCTACCGC TACAGCCGCT CGCTGGAGGA GCTGCTGCTG 
GACGCCAACC AGCTGCGGGA GCTGCCCAAG CCTTTCTTCA GGCTTCTGAA CCTGCGCAAG 
CTGGGCCTGA GTGACAATGA GATCCAAAGA CTTCCCCCTG AGGTGGCCAA CTTCATGCAG 
CTGGTGGAAC TGGACATCTC CCGTAACGAC ATTCCAGAAA TTCCTGAAAG CATCAAATTC 
TGCAAATCCT TGGAAATCGC AGACTTCAGT GGGAATCCTC TCTCCAGGCT TCCCGAGGGC 
TTCACGCAGC TTCGGAGCCT GGGCCATTTG GCCCTGAATG ATGTGTCCCT GCAGAGCCTC 
CCCAGTGACA TTGGGAATTT GGCAAACCTG GTGACTCTGG AGCTACGCGA GAACCTGCTA 
AAGACTCTCC CCACCTCGCT GTCCTTCCTT GTCAAGTTAG AGCAGCTGGA TCTCGGTGGC 
AATGACCTGG AGGTTCTGCC AGACACTCTG GGAGCGCTCC CCAACCTGCG TGAGCTCTGG 
TTAGACCGGA ACCAGCTCTC AGCCCTGCCT CCCGAGCTGG GCAACCTCCG CCGCCTGGTC 
TGCCTGGATG TGTCGGAGAA CAAGCTGGAG CAGTTGCCCA ACGAGGTCAG CGGCCTGGTG 
GCACTGACCG ACTTGCTGCT GTCGCAGAAC CTGTTGGAGT GCATTCCTGA TGGGATTGGT 
CAGCTGAAGC AGCTCTCCAT CCTCAAGGTG GACCAGAATC GACTGACAGA GGTGACAGAG 
TCCATTGGGG ACTGTGAAAA TCTGTCCGAG CTCATCCTGA CAGAGAACAT GCTGACGGCC 
TTGCCGAAAT CCCTTGGGAA GCTGGCCAAG CTGACCAACC TGAACGTGGA TCGGAACCGG 
CTGACATCCC TCCCGGCCGA GATCGGTGGC TGTGCCAACC TCAACGTGCT GTCCTTGCGG 
GACAATCGCC TGACCCTCCT GCCATCGGAG CTGGCCAACA CCACCGAGCT TCATGTTCTG 
GATGTGGCTG GGAACAGGCT GCAGAACTTG CCGTTTGCTC TGACAAACCT CAACCTGAAA 
GCCCTGTGGC TGGCGGAGAA TCAGTCCCAG CCCATGCTGA AATTCCAAAC AGAGGATGAT 
GAGAAGACAG GAGAGAAAGT TCTCACTTGT TACCTGCTTC CTCAGCAGCC CTCTCCCAGC 
CTGGAGAACC TTCTACAGAA CAGCGTGGAC GAGAGCTGGA CGGACACCAA TTTAAACAGA 
GTCAGTGTGA TTCAGTTCCT GGATGAACCC AAAGCAGATG ATGAAGAAGA GTCTGGAGCT 
GAGAGAAGGG GCTTGCAGCG CAGAGCAACC CCCCATCCCA GCGAGCTGAA GGTGATGAAG 
AAGGTGATTG AAGTTCGGCG CAGTGAGGCC CACGCTGCAA AGCCCAATGC AGACCTGAAT 
TCTCCTCACT CAGAAGAGAA GAGGCTGAGC GCCACGTCCA ACCGCAGCGA GGACTCCCAC 
CTTTCCAACA GCACTGCCTC TGCTGACTGT AGGGCGGAGG AGCAGCAGGA GGAGGCAGAG 
AGGAGTCGGC CAAACGGGCA AGTGCCTGAG ATGCAGGAGC TGGAGAAGGA GGCAAAGTCC 
AGAGTGGACG AGGAGCACAA GGAAGCTGCT GGCCATGAAG AGGAAGATGT GGAAGTGGAA 
TACAATGAGC CCACTGTACA CTTTGCTGAA GACACGCTAA TACGGAGTGA GGATGAAGAT 
GAAGATGAAG AGCGACCGTT CCCAGTGGAG AAGCAGCGGC TCATCCGCAA GGACACCCCT 
CACTATAAGA AACACTTTAA GATAACTAAA TTGCCCAAGC CAGAGGCGGT GGTGGCCCTG 
CTGCAGGGGC TGAACAGCGA GGGGGTCCCC AAGGAGGAAG AGGAGTTGGG AGGCTGCAAC 
AACAACACAG AGCCTGCAGA CCAGGAGGAG GTGTGGGATG AGGAGGACCG GAAGAATGGA 
GCTTTGTCTG CCCAGCCATC GGTGAAGGGG GTGTCGTTTG ATCAAGCCAA TAATCTGCTG 
ATTGAACCTG CTCGCATTGA GGAGGAAGAG TTGACTCTGA CTATCGTACG GCAGACGGGG 
GGGCTGGGCA TCAGCATCGC AGGGGGCAAG GGCTCCACCC CCTATAAGGG CGATGATGAG 
GGCATCTTTA TTTCTCGGGT GTCAGAAGAA GGTCCTGCGG CTCGTGCAGG GGTTCGTGTT 
GGGGACAAGC TCCTGGAGGT GAACGGTGTG TCCCTGCACT CCGCTGAGCA CCACGTGGCA 
GTGGAGGCTC TGCGTGGATC GGGAAGTTCC GTCTCCATGA CTGTTCTGCG GGAGCGGATG 
GTGGAGCCGG AGAATGCCAT CACTGTCACC CCGCTGCGCC CCGAGGATGA CTACAGCCCC 
CGTGAGAGGC GAGGTGGCCT GCGCTTCCCG GAGCGACCCG AGGGGACACC TCCCACCGAG 
AGATACTCCA CGTGCCTCAT GCGCAACGAG AAGGGACTGG GCTTCAGTAT CGCTGGTGGC 
AAAGGCTCCA CTCCTTACCG GGCTGGTGAC ATGGGGATCT TTATCTCCCG GATCGCAGAG 
GGTGGTGCAG CCCATCGGGA CGGGATCCTG CACGTAGGAG ATCGGGTCAT CTCGATCAAT 
GGGGTAGACA TGACAGAAGC CAGGCATGAT CAGGCAGTAG CGCTGCTTAC CGCGTCCTCC 
CCCACCATCG TGCTGCTGGT AGAGAGAGAG GGTGCGGAGC AGCCCGGCGA GGGAGACTGG 
CCCGGGGGGC CACGGGCACG CATGCACTCC CCACCGCCAC CCCCCAGCCA TGGGGAGAGC 
CCGCCCGAGG AGACGCCCGC ACCACAGAGG AACCATCTCT CCAAAGGCCT GGAGGATCAG 
TACCCCATTG AGGAAATTCA CCTGGTGAAA GCAGGTGGTC CCCTGGGGCT CAGCATCGTA 
GGGGGCAGTG ACCACTCAAG CCATCCGTTT GGGATTCATG AACCAGGTGT CTTCATTTCG 
AAGGTCATTC CCCGTGGGCT GGCATCCCGC AGTGGGCTGC GAGTAGGGGA CAGGATCCTG 
GAAGTCAATA GCATTGACCT GCGCCACGCT ACCCACCAGG AAGCGGTGAA CGCCCTGCTC 
TCCAACACCC AGGAGCTGAC CATGTTAGTG AGGCGAGACC CACCACCACC TGGTATGCAG 
GAAATATGCA TTGAGAAGGC TCCAGGAGAA AAGCTGGGCA TCAGCATCCG GGGTGGAGCG 
AAAGGCCATG CTGGAAATCC GTTTGATCCC ACTGACGAGG GCATCTTCAT CTCTAAGGTG 
AGCTCCTCGG GGGCGGCAGC CCGGGACGGC CGCCTGAAGG TGGGGATGCG GATCCTAGAG 
GTGAACCACC AGAGCCTGCT GGGGATGACG CACACGGAAG CGGTGCAGAT GCTGCGCGGG 
GTGGGCGACG CGCTCCTGGT GCTGGTGTGT GACGGCTTCG ACCCCAAGGC TGCAGCTGCC 
ATCGAGATGT CCCCGGGAAT TATTGCAAAC CCTTTCGCTG CTGGTATCGG ACGCAAGAAT 
AGCCTGGAAA GTATCTCTTC CATCGACCGG GATTTAAGCC CTGAGGAACT GGAGATCCTG 
CAGAAGGAGA TGGAAATGGT GAGAGAAGCA ACTCAGTGGG AGAGAGAAGA AATGGAGAAA 
GTGGAGCGGA TGCGTAGGGA GCGGGAGGCA GCCACCCGGC AGCTTGAGGA GGAGGCGGAG 
AACGTCACCA GCGAGCCCTT GAGACTGGAC TATCGGACCC TGGCTGCGCT GCCGAGCAGT 
GGTCTCCAGA GAGTCAACCG CACCCTCGTT CAGTATAATA AGCCCAGCTC CAGAATGGAG 
GGCGACTGTG CTCTGCTGTC TCTGCCGGTG GCACGGGACA GCCGGGATTC ATGCCCTGAC 
ACGGCTGCTG GCCCCACCAT CGGAAGCGTG GCCAGACCTC CCCGGGACTC CGCAACACCC 
GACCACTGCA CCAGCGGGGC AGAAACGCCC CGGCCTGACC AGCAGCCTCC CACAGGTGGC 
CCCTTGTCCC AGGACCAGCC GCCAAAGACG CCAGAGCAAG AGTGCATGGT GGACTCCCAG 
CCCATCCTCT TCAGTGAGAA CCCCTTTGTG GTGGCCAACC GCAGGGGGAA GGCGGCCGGC 
CGGGCCTGCC TGGGGGGGCC TCCCCTGGGC TATGGCAGGG GGGGAGTGCT GAAGACCAAC 
CTTTACTCCA AGGCTCCTGC AGGCGAGTCT GGGATGGGCA ATTCCAGGCG TGAAACCCCC 
TATTCTCCTG GTAACCAGCA GCGGCCTGCA ACCCCTCGGC CCGCTCACCT GCCGGGCAGG 
GAGACCCGCT TTGCTTCCAT TAACTTCATT ACATCGCAGG ATGACAGTAA ATCGACCAAG 
CCAGGCGTCA TTCAGCCTCT TTCCCGAGTG CGACAAAATG CTGCCCCGCA CAATTCGGAG 
GAGGGGGACA GCCCCAATCC CTTCCAGCAG CCCGAGCCGT GCTTCAGTGT CCAGAACTCT 
CCAAAGCCAC CCTCACCACC CTCTCCTGAT GAGGTCCCCA TCAATGTCAA GCAAGCGTAC 
AAGACCTTCG CTGCAGTGCC CTTGTCACAC CCCCTGCTTG GGACACAGGA ATTGCCTGGT 
CAGAGCGGCA CAGAGAATCC CAGAAGTGCC CCGGAGGTGG CTGCGCACAG CCCTGGGGGA 
CTGCACAGCC CTGAGCAGAA GTCCTTCCGT GAGAGGCAGA AGTATTTCGA GATTGAGGTG 
AAGCAGCAGC AGATGGACAA GCCCCCCAAG AGAGTCTCCT TGGTGGGAGA GGACGACCTG 
AAGAAGATGA AAGAGGAGGA AGCTCGAAAA CTGCAGCAGA AACGTACCCT TCTGCTGGAG 
GAAGAGGCAG AAGAGGAGGA GATGGCGAAG CAGGCGCCTG AGGTGAGCGT GCAGTCCAGT 
GTCATCATTG AAGGAGTCGA GTACAAGATT GAGAGACTCA ACGGGAGGAG CAGCCAGTCT 
CCGGCCACTC GGCCCTCCGA GGTCAACGAG AACAGCAGCT CGCAGAGGAA TTCACTGGAA 
GAGGGGATCA AGGCTGAGCA GAGAACCAAC TCCATGTCTG GGCTGAGTCC CTTGTACCTT 
GGAGCAGGGG ACGCAGTGGC ACCCGTGCGG ACGGCCAAAG CTGAACGGCG ACACCAGGAG 
CGGTTGCGAA TGCAGAGTCC TGAGCTGCTG AGTGGCCAAG AGAAGGAGCT TTCTCCTGCA 
GAACGTCGTG CTCTGGAGGC GGAGAAACGA GCAATGTGGA GGGCAGCACG ATCCTCAGCT 
CTTGAAGACA GTATTAAACA GTACGAGCAG GACCTGGCCA GGAGGCTGTA CCAGTCCCGA 
CAGCGGGCAC CTGCTCCCGG GGCCGGGCCA GCTCCGATGC CTAGTCCCAA GCAGAGTCAC 
GCCTCGGCAC CCCCTGGGTC AGGAAGCCAT TCCAGGATGA AATCGCTTGA ACAGGATGCT 
CTGAAAGCCC AGATGGTCAT TGCCAAGTCC AAGGAGGGGA AGAAGCGCAG CCCGCTGGAG 
CAGCTGGCAG AGTCCCCCTC GCCAGTTCCT ACACCGTCAC CGACACCACT GGAAGATTGC 
AGTCCCAGGA ACGTCACCTC CCCAGGAAGG CTGTCGCCTG ATGCTGCTGA TGACCTCAGA 
TACATCGAGG ACAGGAATAA CTCAGTTCCC CAGGAGCAGG ATGGGCAGCC CGAGGAAGAG 
GACACATTAG GAACTCACGA CTCATCCACA CCCACTCCAT CAGCACTTGA AGAAATGGCC 
CTGTACAGCA GCAAACGCAA GCTCCGACAC AGCCGGCGCA GCCTGGAGGC TGCCGTCCCT 
ACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCRIB ( XM_014945719.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014801205.1 Calidris pugnax scribbled planar cell polarity protein (SCRIB), transcript variant X6, mRNA.

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CloneID	OCa67752
Clone ID Related Accession (Same CDS sequence)	XM_014945719.1
Accession Version	XM_014945719.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5847bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	protein scribble homolog isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090871.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCCGC TCTGGCGCTG CAACCGGCAC GTGGAGGCGC TGGACCGGCG GCACTGCTCG 
CTGCAGGCCG TGCCCGAGGA GATCTACCGC TACAGCCGCT CGCTGGAGGA GCTGCTGCTG 
GACGCCAACC AGCTGCGGGA GCTGCCCAAG CCTTTCTTCA GGCTTCTGAA CCTGCGCAAG 
CTGGGCCTGA GTGACAATGA GATCCAAAGA CTTCCCCCTG AGGTGGCCAA CTTCATGCAG 
CTGGTGGAAC TGGACATCTC CCGTAACGAC ATTCCAGAAA TTCCTGAAAG CATCAAATTC 
TGCAAATCCT TGGAAATCGC AGACTTCAGT GGGAATCCTC TCTCCAGGCT TCCCGAGGGC 
TTCACGCAGC TTCGGAGCCT GGGCCATTTG GCCCTGAATG ATGTGTCCCT GCAGAGCCTC 
CCCAGTGACA TTGGGAATTT GGCAAACCTG GTGACTCTGG AGCTACGCGA GAACCTGCTA 
AAGACTCTCC CCACCTCGCT GTCCTTCCTT GTCAAGTTAG AGCAGCTGGA TCTCGGTGGC 
AATGACCTGG AGGTTCTGCC AGACACTCTG GGAGCGCTCC CCAACCTGCG TGAGCTCTGG 
TTAGACCGGA ACCAGCTCTC AGCCCTGCCT CCCGAGCTGG GCAACCTCCG CCGCCTGGTC 
TGCCTGGATG TGTCGGAGAA CAAGCTGGAG CAGTTGCCCA ACGAGGTCAG CGGCCTGGTG 
GCACTGACCG ACTTGCTGCT GTCGCAGAAC CTGTTGGAGT GCATTCCTGA TGGGATTGGT 
CAGCTGAAGC AGCTCTCCAT CCTCAAGGTG GACCAGAATC GACTGACAGA GGTGACAGAG 
TCCATTGGGG ACTGTGAAAA TCTGTCCGAG CTCATCCTGA CAGAGAACAT GCTGACGGCC 
TTGCCGAAAT CCCTTGGGAA GCTGGCCAAG CTGACCAACC TGAACGTGGA TCGGAACCGG 
CTGACATCCC TCCCGGCCGA GATCGGTGGC TGTGCCAACC TCAACGTGCT GTCCTTGCGG 
GACAATCGCC TGACCCTCCT GCCATCGGAG CTGGCCAACA CCACCGAGCT TCATGTTCTG 
GATGTGGCTG GGAACAGGCT GCAGAACTTG CCGTTTGCTC TGACAAACCT CAACCTGAAA 
GCCCTGTGGC TGGCGGAGAA TCAGTCCCAG CCCATGCTGA AATTCCAAAC AGAGGATGAT 
GAGAAGACAG GAGAGAAAGT TCTCACTTGT TACCTGCTTC CTCAGCAGCC CTCTCCCAGC 
CTGGAGAACC TTCTACAGAA CAGCGTGGAC GAGAGCTGGA CGGACACCAA TTTAAACAGA 
GTCAGTGTGA TTCAGTTCCT GGATGAACCC AAAGCAGATG ATGAAGAAGA GTCTGGAGCT 
GAGAGAAGGG GCTTGCAGCG CAGAGCAACC CCCCATCCCA GCGAGCTGAA GGTGATGAAG 
AAGGTGATTG AAGTTCGGCG CAGTGAGGCC CACGCTGCAA AGCCCAATGC AGACCTGAAT 
TCTCCTCACT CAGAAGAGAA GAGGCTGAGC GCCACGTCCA ACCGCAGCGA GGACTCCCAC 
CTTTCCAACA GCACTGCCTC TGCTGACTGT AGGGCGGAGG AGCAGCAGGA GGAGGCAGAG 
AGGAGTCGGC CAAACGGGCA AGTGCCTGAG ATGCAGGAGC TGGAGAAGGA GGCAAAGTCC 
AGAGTGGACG AGGAGCACAA GGAAGCTGCT GGCCATGAAG AGGAAGATGT GGAAGTGGAA 
TACAATGAGC CCACTGTACA CTTTGCTGAA GACACGCTAA TACGGAGTGA GGATGAAGAT 
GAAGATGAAG AGCGACCGTT CCCAGTGGAG AAGCAGCGGC TCATCCGCAA GGACACCCCT 
CACTATAAGA AACACTTTAA GATAACTAAA TTGCCCAAGC CAGAGGCGGT GGTGGCCCTG 
CTGCAGGGGC TGAACAGCGA GGGGGTCCCC AAGGAGGAAG AGGAGTTGGG AGGCTGCAAC 
AACAACACAG AGCCTGCAGA CCAGGAGGAG GTGTGGGATG AGGAGGACCG GAAGAATGGA 
GCTTTGTCTG CCCAGCCATC GGTGAAGGGG GTGTCGTTTG ATCAAGCCAA TAATCTGCTG 
ATTGAACCTG CTCGCATTGA GGAGGAAGAG TTGACTCTGA CTATCGTACG GCAGACGGGG 
GGGCTGGGCA TCAGCATCGC AGGGGGCAAG GGCTCCACCC CCTATAAGGG CGATGATGAG 
GGCATCTTTA TTTCTCGGGT GTCAGAAGAA GGTCCTGCGG CTCGTGCAGG GGTTCGTGTT 
GGGGACAAGC TCCTGGAGGT GAACGGTGTG TCCCTGCACT CCGCTGAGCA CCACGTGGCA 
GTGGAGGCTC TGCGTGGATC GGGAAGTTCC GTCTCCATGA CTGTTCTGCG GGAGCGGATG 
GTGGAGCCGG AGAATGCCAT CACTGTCACC CCGCTGCGCC CCGAGGATGA CTACAGCCCC 
CGTGAGAGGC GAGGTGGCCT GCGCTTCCCG GAGCGACCCG AGGGGACACC TCCCACCGAG 
AGATACTCCA CGTGCCTCAT GCGCAACGAG AAGGGACTGG GCTTCAGTAT CGCTGGTGGC 
AAAGGCTCCA CTCCTTACCG GGCTGGTGAC ATGGGGATCT TTATCTCCCG GATCGCAGAG 
GGTGGTGCAG CCCATCGGGA CGGGATCCTG CACGTAGGAG ATCGGGTCAT CTCGATCAAT 
GGGGTAGACA TGACAGAAGC CAGGCATGAT CAGGCAGTAG CGCTGCTTAC CGCGTCCTCC 
CCCACCATCG TGCTGCTGGT AGAGAGAGAG GGTGCGGAGC AGCCCGGCGA GGGAGACTGG 
CCCGGGGGGC CACGGGCACG CATGCACTCC CCACCGCCAC CCCCCAGCCA TGGGGAGAGC 
CCGCCCGAGG AGACGCCCGC ACCACAGAGG AACCATCTCT CCAAAGGCCT GGAGGATCAG 
TACCCCATTG AGGAAATTCA CCTGGTGAAA GCAGGTGGTC CCCTGGGGCT CAGCATCGTA 
GGGGGCAGTG ACCACTCAAG CCATCCGTTT GGGATTCATG AACCAGGTGT CTTCATTTCG 
AAGGTCATTC CCCGTGGGCT GGCATCCCGC AGTGGGCTGC GAGTAGGGGA CAGGATCCTG 
GAAGTCAATA GCATTGACCT GCGCCACGCT ACCCACCAGG AAGCGGTGAA CGCCCTGCTC 
TCCAACACCC AGGAGCTGAC CATGTTAGTG AGGCGAGACC CACCACCACC TGGTATGCAG 
GAAATATGCA TTGAGAAGGC TCCAGGAGAA AAGCTGGGCA TCAGCATCCG GGGTGGAGCG 
AAAGGCCATG CTGGAAATCC GTTTGATCCC ACTGACGAGG GCATCTTCAT CTCTAAGGTG 
AGCTCCTCGG GGGCGGCAGC CCGGGACGGC CGCCTGAAGG TGGGGATGCG GATCCTAGAG 
GTGAACCACC AGAGCCTGCT GGGGATGACG CACACGGAAG CGGTGCAGAT GCTGCGCGGG 
GTGGGCGACG CGCTCCTGGT GCTGGTGTGT GACGGCTTCG ACCCCAAGGC TGCAGCTGCC 
ATCGAGATGT CCCCGGGAAT TATTGCAAAC CCTTTCGCTG CTGGTATCGG ACGCAAGAAT 
AGCCTGGAAA GTATCTCTTC CATCGACCGG GATTTAAGCC CTGAGGAACT GGAGATCCTG 
CAGAAGAACG TCACCAGCGA GCCCTTGAGA CTGGACTATC GGACCCTGGC TGCGCTGCCG 
AGCAGTGGTC TCCAGAGAGT CAACCGCACC CTCGTTCAGT ATAATAAGCC CAGCTCCAGA 
ATGGAGGGCG ACTGTGCTCT GCTGTCTCTG CCGGTGGCAC GGGACAGCCG GGATTCATGC 
CCTGACACGG CTGCTGGCCC CACCATCGGA AGCGTGGCCA GACCTCCCCG GGACTCCGCA 
ACACCCGACC ACTGCACCAG CGGGGCAGAA ACGCCCCGGC CTGACCAGCA GCCTCCCACA 
GGTGGCCCCT TGTCCCAGGA CCAGCCGCCA AAGACGCCAG AGCAAGAGTG CATGGTGGAC 
TCCCAGCCCA TCCTCTTCAG TGAGAACCCC TTTGTGGTGG CCAACCGCAG GGGGAAGGCG 
GCCGGCCGGG CCTGCCTGGG GGGGCCTCCC CTGGGCTATG GCAGGGGGGG AGTGCTGAAG 
ACCAACCTTT ACTCCAAGGC TCCTGCAGGC GAGTCTGGGA TGGGCAATTC CAGGCGTGAA 
ACCCCCTATT CTCCTGGTAA CCAGCAGCGG CCTGCAACCC CTCGGCCCGC TCACCTGCCG 
GGCAGGGAGA CCCGCTTTGC TTCCATTAAC TTCATTACAT CGCAGGATGA CAGTAAATCG 
ACCAAGCCAG GCGTCATTCA GCCTCTTTCC CGAGTGCGAC AAAATGCTGC CCCGCACAAT 
TCGGAGGAGG GGGACAGCCC CAATCCCTTC CAGCAGCCCG AGCCGTGCTT CAGTGTCCAG 
AACTCTCCAA AGCCACCCTC ACCACCCTCT CCTGATGAGG TCCCCATCAA TGTCAAGCAA 
GCGTACAAGA CCTTCGCTGC AGTGCCCTTG TCACACCCCC TGCTTGGGAC ACAGGAATTG 
CCTGGTCAGA GCGGCACAGA GAATCCCAGA AGTGCCCCGG AGGTGGCTGC GCACAGCCCT 
GGGGGACTGC ACAGCCCTGA GCAGAAGTCC TTCCGTGAGA GGCAGAAGTA TTTCGAGATT 
GAGGTGAAGC AGCAGCAGAT GGACAAGCCC CCCAAGAGAG TCTCCTTGGT GGGAGAGGAC 
GACCTGAAGA AGATGAAAGA GGAGGAAGCT CGAAAACTGC AGCAGAAACG TACCCTTCTG 
CTGGAGGAAG AGGCAGAAGA GGAGGAGATG GCGAAGCAGG CGCCTGAGGT GAGCGTGCAG 
TCCAGTGTCA TCATTGAAGG AGTCGAGTAC AAGATTGAGA GACTCAACGG GAGGAGCAGC 
CAGTCTCCGG CCACTCGGCC CTCCGAGGTC AACGAGAACA GCAGCTCGCA GAGGAATTCA 
CTGGAAGAGG GGATCAAGGC TGAGCAGAGA ACCAACTCCA TGTCTGGGCT GAGTCCCTTG 
TACCTTGGAG CAGGGGACGC AGTGGCACCC GTGCGGACGG CCAAAGCTGA ACGGCGACAC 
CAGGAGCGGT TGCGAATGCA GAGTCCTGAG CTGCTGAGTG GCCAAGAGAA GGAGCTTTCT 
CCTGCAGAAC GTCGTGCTCT GGAGGCGGAG AAACGAGCAA TGTGGAGGGC AGCACGATCC 
TCAGCTCTTG AAGACAGTAT TAAACAGTAC GAGCAGGACC TGGCCAGGAG GCTGTACCAG 
TCCCGACAGC GGGCACCTGC TCCCGGGGCC GGGCCAGCTC CGATGCCTAG TCCCAAGCAG 
AGTCACGCCT CGGCACCCCC TGGGTCAGGA AGCCATTCCA GGATGAAATC GCTTGAACAG 
GATGCTCTGA AAGCCCAGAT GGTCATTGCC AAGTCCAAGG AGGGGAAGAA GCGCAGCCCG 
CTGGAGCAGC TGGCAGAGTC CCCCTCGCCA GTTCCTACAC CGTCACCGAC ACCACTGGAA 
GATTGCAGTC CCAGGAACGT CACCTCCCCA GGAAGGCTGT CCATGTCTGA AAAGAAGTTT 
GACTACCGGG AATTTGCTGC TATTCCTTCC TCCAAACCCG TTTACGAAAT TCAGTCGCCT 
GATGCTGCTG ATGACCTCAG ATACATCGAG GACAGGAATA ACTCAGTTCC CCAGGAGCAG 
GATGGGCAGC CCGAGGAAGA GGACACATTA GGAACTCACG ACTCATCCAC ACCCACTCCA 
TCAGCACTTG AAGAAATGGC CCTGTACAGC AGCAAACGCA AGCTCCGACA CAGCCGGCGC 
AGCCTGGAGG CTGCCGTCCC TACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014801205.1
CDS	6..5852
Translation	MPPLWRCNRHVEALDRRHCSLQAVPEEIYRYSRSLEELLLDANQLRELPKPFFRLLNLRKLGLSDNEIQRLPPEVANFMQLVELDISRNDIPEIPESIKFCKSLEIADFSGNPLSRLPEGFTQLRSLGHLALNDVSLQSLPSDIGNLANLVTLELRENLLKTLPTSLSFLVKLEQLDLGGNDLEVLPDTLGALPNLRELWLDRNQLSALPPELGNLRRLVCLDVSENKLEQLPNEVSGLVALTDLLLSQNLLECIPDGIGQLKQLSILKVDQNRLTEVTESIGDCENLSELILTENMLTALPKSLGKLAKLTNLNVDRNRLTSLPAEIGGCANLNVLSLRDNRLTLLPSELANTTELHVLDVAGNRLQNLPFALTNLNLKALWLAENQSQPMLKFQTEDDEKTGEKVLTCYLLPQQPSPSLENLLQNSVDESWTDTNLNRVSVIQFLDEPKADDEEESGAERRGLQRRATPHPSELKVMKKVIEVRRSEAHAAKPNADLNSPHSEEKRLSATSNRSEDSHLSNSTASADCRAEEQQEEAERSRPNGQVPEMQELEKEAKSRVDEEHKEAAGHEEEDVEVEYNEPTVHFAEDTLIRSEDEDEDEERPFPVEKQRLIRKDTPHYKKHFKITKLPKPEAVVALLQGLNSEGVPKEEEELGGCNNNTEPADQEEVWDEEDRKNGALSAQPSVKGVSFDQANNLLIEPARIEEEELTLTIVRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVRVGDKLLEVNGVSLHSAEHHVAVEALRGSGSSVSMTVLRERMVEPENAITVTPLRPEDDYSPRERRGGLRFPERPEGTPPTERYSTCLMRNEKGLGFSIAGGKGSTPYRAGDMGIFISRIAEGGAAHRDGILHVGDRVISINGVDMTEARHDQAVALLTASSPTIVLLVEREGAEQPGEGDWPGGPRARMHSPPPPPSHGESPPEETPAPQRNHLSKGLEDQYPIEEIHLVKAGGPLGLSIVGGSDHSSHPFGIHEPGVFISKVIPRGLASRSGLRVGDRILEVNSIDLRHATHQEAVNALLSNTQELTMLVRRDPPPPGMQEICIEKAPGEKLGISIRGGAKGHAGNPFDPTDEGIFISKVSSSGAAARDGRLKVGMRILEVNHQSLLGMTHTEAVQMLRGVGDALLVLVCDGFDPKAAAAIEMSPGIIANPFAAGIGRKNSLESISSIDRDLSPEELEILQKNVTSEPLRLDYRTLAALPSSGLQRVNRTLVQYNKPSSRMEGDCALLSLPVARDSRDSCPDTAAGPTIGSVARPPRDSATPDHCTSGAETPRPDQQPPTGGPLSQDQPPKTPEQECMVDSQPILFSENPFVVANRRGKAAGRACLGGPPLGYGRGGVLKTNLYSKAPAGESGMGNSRRETPYSPGNQQRPATPRPAHLPGRETRFASINFITSQDDSKSTKPGVIQPLSRVRQNAAPHNSEEGDSPNPFQQPEPCFSVQNSPKPPSPPSPDEVPINVKQAYKTFAAVPLSHPLLGTQELPGQSGTENPRSAPEVAAHSPGGLHSPEQKSFRERQKYFEIEVKQQQMDKPPKRVSLVGEDDLKKMKEEEARKLQQKRTLLLEEEAEEEEMAKQAPEVSVQSSVIIEGVEYKIERLNGRSSQSPATRPSEVNENSSSQRNSLEEGIKAEQRTNSMSGLSPLYLGAGDAVAPVRTAKAERRHQERLRMQSPELLSGQEKELSPAERRALEAEKRAMWRAARSSALEDSIKQYEQDLARRLYQSRQRAPAPGAGPAPMPSPKQSHASAPPGSGSHSRMKSLEQDALKAQMVIAKSKEGKKRSPLEQLAESPSPVPTPSPTPLEDCSPRNVTSPGRLSMSEKKFDYREFAAIPSSKPVYEIQSPDAADDLRYIEDRNNSVPQEQDGQPEEEDTLGTHDSSTPTPSALEEMALYSSKRKLRHSRRSLEAAVPT

Target ORF information:

RefSeq Version	XM_014945719.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax scribbled planar cell polarity protein (SCRIB), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014945719.1

ORF Insert Sequence:

ATGCCGCCGC TCTGGCGCTG CAACCGGCAC GTGGAGGCGC TGGACCGGCG GCACTGCTCG 
CTGCAGGCCG TGCCCGAGGA GATCTACCGC TACAGCCGCT CGCTGGAGGA GCTGCTGCTG 
GACGCCAACC AGCTGCGGGA GCTGCCCAAG CCTTTCTTCA GGCTTCTGAA CCTGCGCAAG 
CTGGGCCTGA GTGACAATGA GATCCAAAGA CTTCCCCCTG AGGTGGCCAA CTTCATGCAG 
CTGGTGGAAC TGGACATCTC CCGTAACGAC ATTCCAGAAA TTCCTGAAAG CATCAAATTC 
TGCAAATCCT TGGAAATCGC AGACTTCAGT GGGAATCCTC TCTCCAGGCT TCCCGAGGGC 
TTCACGCAGC TTCGGAGCCT GGGCCATTTG GCCCTGAATG ATGTGTCCCT GCAGAGCCTC 
CCCAGTGACA TTGGGAATTT GGCAAACCTG GTGACTCTGG AGCTACGCGA GAACCTGCTA 
AAGACTCTCC CCACCTCGCT GTCCTTCCTT GTCAAGTTAG AGCAGCTGGA TCTCGGTGGC 
AATGACCTGG AGGTTCTGCC AGACACTCTG GGAGCGCTCC CCAACCTGCG TGAGCTCTGG 
TTAGACCGGA ACCAGCTCTC AGCCCTGCCT CCCGAGCTGG GCAACCTCCG CCGCCTGGTC 
TGCCTGGATG TGTCGGAGAA CAAGCTGGAG CAGTTGCCCA ACGAGGTCAG CGGCCTGGTG 
GCACTGACCG ACTTGCTGCT GTCGCAGAAC CTGTTGGAGT GCATTCCTGA TGGGATTGGT 
CAGCTGAAGC AGCTCTCCAT CCTCAAGGTG GACCAGAATC GACTGACAGA GGTGACAGAG 
TCCATTGGGG ACTGTGAAAA TCTGTCCGAG CTCATCCTGA CAGAGAACAT GCTGACGGCC 
TTGCCGAAAT CCCTTGGGAA GCTGGCCAAG CTGACCAACC TGAACGTGGA TCGGAACCGG 
CTGACATCCC TCCCGGCCGA GATCGGTGGC TGTGCCAACC TCAACGTGCT GTCCTTGCGG 
GACAATCGCC TGACCCTCCT GCCATCGGAG CTGGCCAACA CCACCGAGCT TCATGTTCTG 
GATGTGGCTG GGAACAGGCT GCAGAACTTG CCGTTTGCTC TGACAAACCT CAACCTGAAA 
GCCCTGTGGC TGGCGGAGAA TCAGTCCCAG CCCATGCTGA AATTCCAAAC AGAGGATGAT 
GAGAAGACAG GAGAGAAAGT TCTCACTTGT TACCTGCTTC CTCAGCAGCC CTCTCCCAGC 
CTGGAGAACC TTCTACAGAA CAGCGTGGAC GAGAGCTGGA CGGACACCAA TTTAAACAGA 
GTCAGTGTGA TTCAGTTCCT GGATGAACCC AAAGCAGATG ATGAAGAAGA GTCTGGAGCT 
GAGAGAAGGG GCTTGCAGCG CAGAGCAACC CCCCATCCCA GCGAGCTGAA GGTGATGAAG 
AAGGTGATTG AAGTTCGGCG CAGTGAGGCC CACGCTGCAA AGCCCAATGC AGACCTGAAT 
TCTCCTCACT CAGAAGAGAA GAGGCTGAGC GCCACGTCCA ACCGCAGCGA GGACTCCCAC 
CTTTCCAACA GCACTGCCTC TGCTGACTGT AGGGCGGAGG AGCAGCAGGA GGAGGCAGAG 
AGGAGTCGGC CAAACGGGCA AGTGCCTGAG ATGCAGGAGC TGGAGAAGGA GGCAAAGTCC 
AGAGTGGACG AGGAGCACAA GGAAGCTGCT GGCCATGAAG AGGAAGATGT GGAAGTGGAA 
TACAATGAGC CCACTGTACA CTTTGCTGAA GACACGCTAA TACGGAGTGA GGATGAAGAT 
GAAGATGAAG AGCGACCGTT CCCAGTGGAG AAGCAGCGGC TCATCCGCAA GGACACCCCT 
CACTATAAGA AACACTTTAA GATAACTAAA TTGCCCAAGC CAGAGGCGGT GGTGGCCCTG 
CTGCAGGGGC TGAACAGCGA GGGGGTCCCC AAGGAGGAAG AGGAGTTGGG AGGCTGCAAC 
AACAACACAG AGCCTGCAGA CCAGGAGGAG GTGTGGGATG AGGAGGACCG GAAGAATGGA 
GCTTTGTCTG CCCAGCCATC GGTGAAGGGG GTGTCGTTTG ATCAAGCCAA TAATCTGCTG 
ATTGAACCTG CTCGCATTGA GGAGGAAGAG TTGACTCTGA CTATCGTACG GCAGACGGGG 
GGGCTGGGCA TCAGCATCGC AGGGGGCAAG GGCTCCACCC CCTATAAGGG CGATGATGAG 
GGCATCTTTA TTTCTCGGGT GTCAGAAGAA GGTCCTGCGG CTCGTGCAGG GGTTCGTGTT 
GGGGACAAGC TCCTGGAGGT GAACGGTGTG TCCCTGCACT CCGCTGAGCA CCACGTGGCA 
GTGGAGGCTC TGCGTGGATC GGGAAGTTCC GTCTCCATGA CTGTTCTGCG GGAGCGGATG 
GTGGAGCCGG AGAATGCCAT CACTGTCACC CCGCTGCGCC CCGAGGATGA CTACAGCCCC 
CGTGAGAGGC GAGGTGGCCT GCGCTTCCCG GAGCGACCCG AGGGGACACC TCCCACCGAG 
AGATACTCCA CGTGCCTCAT GCGCAACGAG AAGGGACTGG GCTTCAGTAT CGCTGGTGGC 
AAAGGCTCCA CTCCTTACCG GGCTGGTGAC ATGGGGATCT TTATCTCCCG GATCGCAGAG 
GGTGGTGCAG CCCATCGGGA CGGGATCCTG CACGTAGGAG ATCGGGTCAT CTCGATCAAT 
GGGGTAGACA TGACAGAAGC CAGGCATGAT CAGGCAGTAG CGCTGCTTAC CGCGTCCTCC 
CCCACCATCG TGCTGCTGGT AGAGAGAGAG GGTGCGGAGC AGCCCGGCGA GGGAGACTGG 
CCCGGGGGGC CACGGGCACG CATGCACTCC CCACCGCCAC CCCCCAGCCA TGGGGAGAGC 
CCGCCCGAGG AGACGCCCGC ACCACAGAGG AACCATCTCT CCAAAGGCCT GGAGGATCAG 
TACCCCATTG AGGAAATTCA CCTGGTGAAA GCAGGTGGTC CCCTGGGGCT CAGCATCGTA 
GGGGGCAGTG ACCACTCAAG CCATCCGTTT GGGATTCATG AACCAGGTGT CTTCATTTCG 
AAGGTCATTC CCCGTGGGCT GGCATCCCGC AGTGGGCTGC GAGTAGGGGA CAGGATCCTG 
GAAGTCAATA GCATTGACCT GCGCCACGCT ACCCACCAGG AAGCGGTGAA CGCCCTGCTC 
TCCAACACCC AGGAGCTGAC CATGTTAGTG AGGCGAGACC CACCACCACC TGGTATGCAG 
GAAATATGCA TTGAGAAGGC TCCAGGAGAA AAGCTGGGCA TCAGCATCCG GGGTGGAGCG 
AAAGGCCATG CTGGAAATCC GTTTGATCCC ACTGACGAGG GCATCTTCAT CTCTAAGGTG 
AGCTCCTCGG GGGCGGCAGC CCGGGACGGC CGCCTGAAGG TGGGGATGCG GATCCTAGAG 
GTGAACCACC AGAGCCTGCT GGGGATGACG CACACGGAAG CGGTGCAGAT GCTGCGCGGG 
GTGGGCGACG CGCTCCTGGT GCTGGTGTGT GACGGCTTCG ACCCCAAGGC TGCAGCTGCC 
ATCGAGATGT CCCCGGGAAT TATTGCAAAC CCTTTCGCTG CTGGTATCGG ACGCAAGAAT 
AGCCTGGAAA GTATCTCTTC CATCGACCGG GATTTAAGCC CTGAGGAACT GGAGATCCTG 
CAGAAGAACG TCACCAGCGA GCCCTTGAGA CTGGACTATC GGACCCTGGC TGCGCTGCCG 
AGCAGTGGTC TCCAGAGAGT CAACCGCACC CTCGTTCAGT ATAATAAGCC CAGCTCCAGA 
ATGGAGGGCG ACTGTGCTCT GCTGTCTCTG CCGGTGGCAC GGGACAGCCG GGATTCATGC 
CCTGACACGG CTGCTGGCCC CACCATCGGA AGCGTGGCCA GACCTCCCCG GGACTCCGCA 
ACACCCGACC ACTGCACCAG CGGGGCAGAA ACGCCCCGGC CTGACCAGCA GCCTCCCACA 
GGTGGCCCCT TGTCCCAGGA CCAGCCGCCA AAGACGCCAG AGCAAGAGTG CATGGTGGAC 
TCCCAGCCCA TCCTCTTCAG TGAGAACCCC TTTGTGGTGG CCAACCGCAG GGGGAAGGCG 
GCCGGCCGGG CCTGCCTGGG GGGGCCTCCC CTGGGCTATG GCAGGGGGGG AGTGCTGAAG 
ACCAACCTTT ACTCCAAGGC TCCTGCAGGC GAGTCTGGGA TGGGCAATTC CAGGCGTGAA 
ACCCCCTATT CTCCTGGTAA CCAGCAGCGG CCTGCAACCC CTCGGCCCGC TCACCTGCCG 
GGCAGGGAGA CCCGCTTTGC TTCCATTAAC TTCATTACAT CGCAGGATGA CAGTAAATCG 
ACCAAGCCAG GCGTCATTCA GCCTCTTTCC CGAGTGCGAC AAAATGCTGC CCCGCACAAT 
TCGGAGGAGG GGGACAGCCC CAATCCCTTC CAGCAGCCCG AGCCGTGCTT CAGTGTCCAG 
AACTCTCCAA AGCCACCCTC ACCACCCTCT CCTGATGAGG TCCCCATCAA TGTCAAGCAA 
GCGTACAAGA CCTTCGCTGC AGTGCCCTTG TCACACCCCC TGCTTGGGAC ACAGGAATTG 
CCTGGTCAGA GCGGCACAGA GAATCCCAGA AGTGCCCCGG AGGTGGCTGC GCACAGCCCT 
GGGGGACTGC ACAGCCCTGA GCAGAAGTCC TTCCGTGAGA GGCAGAAGTA TTTCGAGATT 
GAGGTGAAGC AGCAGCAGAT GGACAAGCCC CCCAAGAGAG TCTCCTTGGT GGGAGAGGAC 
GACCTGAAGA AGATGAAAGA GGAGGAAGCT CGAAAACTGC AGCAGAAACG TACCCTTCTG 
CTGGAGGAAG AGGCAGAAGA GGAGGAGATG GCGAAGCAGG CGCCTGAGGT GAGCGTGCAG 
TCCAGTGTCA TCATTGAAGG AGTCGAGTAC AAGATTGAGA GACTCAACGG GAGGAGCAGC 
CAGTCTCCGG CCACTCGGCC CTCCGAGGTC AACGAGAACA GCAGCTCGCA GAGGAATTCA 
CTGGAAGAGG GGATCAAGGC TGAGCAGAGA ACCAACTCCA TGTCTGGGCT GAGTCCCTTG 
TACCTTGGAG CAGGGGACGC AGTGGCACCC GTGCGGACGG CCAAAGCTGA ACGGCGACAC 
CAGGAGCGGT TGCGAATGCA GAGTCCTGAG CTGCTGAGTG GCCAAGAGAA GGAGCTTTCT 
CCTGCAGAAC GTCGTGCTCT GGAGGCGGAG AAACGAGCAA TGTGGAGGGC AGCACGATCC 
TCAGCTCTTG AAGACAGTAT TAAACAGTAC GAGCAGGACC TGGCCAGGAG GCTGTACCAG 
TCCCGACAGC GGGCACCTGC TCCCGGGGCC GGGCCAGCTC CGATGCCTAG TCCCAAGCAG 
AGTCACGCCT CGGCACCCCC TGGGTCAGGA AGCCATTCCA GGATGAAATC GCTTGAACAG 
GATGCTCTGA AAGCCCAGAT GGTCATTGCC AAGTCCAAGG AGGGGAAGAA GCGCAGCCCG 
CTGGAGCAGC TGGCAGAGTC CCCCTCGCCA GTTCCTACAC CGTCACCGAC ACCACTGGAA 
GATTGCAGTC CCAGGAACGT CACCTCCCCA GGAAGGCTGT CCATGTCTGA AAAGAAGTTT 
GACTACCGGG AATTTGCTGC TATTCCTTCC TCCAAACCCG TTTACGAAAT TCAGTCGCCT 
GATGCTGCTG ATGACCTCAG ATACATCGAG GACAGGAATA ACTCAGTTCC CCAGGAGCAG 
GATGGGCAGC CCGAGGAAGA GGACACATTA GGAACTCACG ACTCATCCAC ACCCACTCCA 
TCAGCACTTG AAGAAATGGC CCTGTACAGC AGCAAACGCA AGCTCCGACA CAGCCGGCGC 
AGCCTGGAGG CTGCCGTCCC TACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCRIB ( XM_014945720.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014801206.1 Calidris pugnax scribbled planar cell polarity protein (SCRIB), transcript variant X7, mRNA.

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CloneID	OCa67753
Clone ID Related Accession (Same CDS sequence)	XM_014945720.1
Accession Version	XM_014945720.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5835bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	protein scribble homolog isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090871.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCCGC TCTGGCGCTG CAACCGGCAC GTGGAGGCGC TGGACCGGCG GCACTGCTCG 
CTGCAGGCCG TGCCCGAGGA GATCTACCGC TACAGCCGCT CGCTGGAGGA GCTGCTGCTG 
GACGCCAACC AGCTGCGGGA GCTGCCCAAG CCTTTCTTCA GGCTTCTGAA CCTGCGCAAG 
CTGGGCCTGA GTGACAATGA GATCCAAAGA CTTCCCCCTG AGGTGGCCAA CTTCATGCAG 
CTGGTGGAAC TGGACATCTC CCGTAACGAC ATTCCAGAAA TTCCTGAAAG CATCAAATTC 
TGCAAATCCT TGGAAATCGC AGACTTCAGT GGGAATCCTC TCTCCAGGCT TCCCGAGGGC 
TTCACGCAGC TTCGGAGCCT GGGCCATTTG GCCCTGAATG ATGTGTCCCT GCAGAGCCTC 
CCCAGTGACA TTGGGAATTT GGCAAACCTG GTGACTCTGG AGCTACGCGA GAACCTGCTA 
AAGACTCTCC CCACCTCGCT GTCCTTCCTT GTCAAGTTAG AGCAGCTGGA TCTCGGTGGC 
AATGACCTGG AGGTTCTGCC AGACACTCTG GGAGCGCTCC CCAACCTGCG TGAGCTCTGG 
TTAGACCGGA ACCAGCTCTC AGCCCTGCCT CCCGAGCTGG GCAACCTCCG CCGCCTGGTC 
TGCCTGGATG TGTCGGAGAA CAAGCTGGAG CAGTTGCCCA ACGAGGTCAG CGGCCTGGTG 
GCACTGACCG ACTTGCTGCT GTCGCAGAAC CTGTTGGAGT GCATTCCTGA TGGGATTGGT 
CAGCTGAAGC AGCTCTCCAT CCTCAAGGTG GACCAGAATC GACTGACAGA GGTGACAGAG 
TCCATTGGGG ACTGTGAAAA TCTGTCCGAG CTCATCCTGA CAGAGAACAT GCTGACGGCC 
TTGCCGAAAT CCCTTGGGAA GCTGGCCAAG CTGACCAACC TGAACGTGGA TCGGAACCGG 
CTGACATCCC TCCCGGCCGA GATCGGTGGC TGTGCCAACC TCAACGTGCT GTCCTTGCGG 
GACAATCGCC TGACCCTCCT GCCATCGGAG CTGGCCAACA CCACCGAGCT TCATGTTCTG 
GATGTGGCTG GGAACAGGCT GCAGAACTTG CCGTTTGCTC TGACAAACCT CAACCTGAAA 
GCCCTGTGGC TGGCGGAGAA TCAGTCCCAG CCCATGCTGA AATTCCAAAC AGAGGATGAT 
GAGAAGACAG GAGAGAAAGT TCTCACTTGT TACCTGCTTC CTCAGCAGCC CTCTCCCAGC 
CTGGAGAACC TTCTACAGAA CAGCGTGGAC GAGAGCTGGA CGGACACCAA TTTAAACAGA 
GTCAGTGTGA TTCAGTTCCT GGATGAACCC AAAGCAGATG ATGAAGAAGA GTCTGGAGCT 
GAGAGAAGGG GCTTGCAGCG CAGAGCAACC CCCCATCCCA GCGAGCTGAA GGTGATGAAG 
AAGGTGATTG AAGTTCGGCG CAGTGAGGCC CACGCTGCAA AGCCCAATGC AGACCTGAAT 
TCTCCTCACT CAGAAGAGAA GAGGCTGAGC GCCACGTCCA ACCGCAGCGA GGACTCCCAC 
CTTTCCAACA GCACTGCCTC TGCTGACTGT AGGGCGGAGG AGCAGCAGGA GGAGGCAGAG 
AGGAGTCGGC CAAACGGGCA AGTGCCTGAG ATGCAGGAGC TGGAGAAGGA GGCAAAGTCC 
AGAGTGGACG AGGAGCACAA GGAAGCTGCT GGCCATGAAG AGGAAGATGT GGAAGTGGAA 
TACAATGAGC CCACTGTACA CTTTGCTGAA GACACGCTAA TACGGAGTGA GGATGAAGAT 
GAAGATGAAG AGCGACCGTT CCCAGTGGAG AAGCAGCGGC TCATCCGCAA GGACACCCCT 
CACTATAAGA AACACTTTAA GATAACTAAA TTGCCCAAGC CAGAGGCGGT GGTGGCCCTG 
CTGCAGGGGC TGAACAGCGA GGGGGTCCCC AAGGAGGAAG AGGAGTTGGG AGGCTGCAAC 
AACAACACAG AGCCTGCAGA CCAGGAGGAG GTGTGGGATG AGGAGGACCG GAAGAATGGA 
GCTTTGTCTG CCCAGCCATC GGTGAAGGGG GTGTCGTTTG ATCAAGCCAA TAATCTGCTG 
ATTGAACCTG CTCGCATTGA GGAGGAAGAG TTGACTCTGA CTATCGTACG GCAGACGGGG 
GGGCTGGGCA TCAGCATCGC AGGGGGCAAG GGCTCCACCC CCTATAAGGG CGATGATGAG 
GGCATCTTTA TTTCTCGGGT GTCAGAAGAA GGTCCTGCGG CTCGTGCAGG GGTTCGTGTT 
GGGGACAAGC TCCTGGAGGT GAACGGTGTG TCCCTGCACT CCGCTGAGCA CCACGTGGCA 
GTGGAGGCTC TGCGTGGATC GGGAAGTTCC GTCTCCATGA CTGTTCTGCG GGAGCGGATG 
GTGGAGCCGG AGAATGCCAT CACTGTCACC CCGCTGCGCC CCGAGGATGA CTACAGCCCC 
CGTGAGAGGC GAGGTGGCCT GCGCTTCCCG GAGCGACCCG AGGGGACACC TCCCACCGAG 
AGATACTCCA CGTGCCTCAT GCGCAACGAG AAGGGACTGG GCTTCAGTAT CGCTGGTGGC 
AAAGGCTCCA CTCCTTACCG GGCTGGTGAC ATGGGGATCT TTATCTCCCG GATCGCAGAG 
GGTGGTGCAG CCCATCGGGA CGGGATCCTG CACGTAGGAG ATCGGGTCAT CTCGATCAAT 
GGGGTAGACA TGACAGAAGC CAGGCATGAT CAGGCAGTAG CGCTGCTTAC CGCGTCCTCC 
CCCACCATCG TGCTGCTGGT AGAGAGAGAG GGTGCGGAGC AGCCCGGCGA GGGAGACTGG 
CCCGGGGGGC CACGGGCACG CATGCACTCC CCACCGCCAC CCCCCAGCCA TGGGGAGAGC 
CCGCCCGAGG AGACGCCCGC ACCACAGAGG AACCATCTCT CCAAAGGCCT GGAGGATCAG 
TACCCCATTG AGGAAATTCA CCTGGTGAAA GCAGGTGGTC CCCTGGGGCT CAGCATCGTA 
GGGGGCAGTG ACCACTCAAG CCATCCGTTT GGGATTCATG AACCAGGTGT CTTCATTTCG 
AAGGTCATTC CCCGTGGGCT GGCATCCCGC AGTGGGCTGC GAGTAGGGGA CAGGATCCTG 
GAAGTCAATA GCATTGACCT GCGCCACGCT ACCCACCAGG AAGCGGTGAA CGCCCTGCTC 
TCCAACACCC AGGAGCTGAC CATGTTAGTG AGGCGAGACC CACCACCACC TGGTATGCAG 
GAAATATGCA TTGAGAAGGC TCCAGGAGAA AAGCTGGGCA TCAGCATCCG GGGTGGAGCG 
AAAGGCCATG CTGGAAATCC GTTTGATCCC ACTGACGAGG GCATCTTCAT CTCTAAGGTG 
AGCTCCTCGG GGGCGGCAGC CCGGGACGGC CGCCTGAAGG TGGGGATGCG GATCCTAGAG 
GTGAACCACC AGAGCCTGCT GGGGATGACG CACACGGAAG CGGTGCAGAT GCTGCGCGGG 
GTGGGCGACG CGCTCCTGGT GCTGGTGTGT GACGGCTTCG ACCCCAAGGC TGCAGCTGCC 
ATCGAGATGT CCCCGGGAAT TATTGCAAAC CCTTTCGCTG CTGGTATCGG ACGCAAGAAT 
AGCCTGGAAA GTATCTCTTC CATCGACCGG GATTTAAGCC CTGAGGAACT GGAGATCCTG 
CAGAAGGAGA TGGAAATGGT GAGAGAAGCA ACTCAGTGGG AGAGAGAAGA AATGGAGAAA 
GTGGAGCGGA TGCGTAGGGA GCGGGAGGCA GCCACCCGGC AGCTTGAGGA GGAGGCGGAG 
AACGTCACCA GCGAGCCCTT GAGACTGGAC TATCGGACCC TGGCTGCGCT GCCGAGCAGT 
GGTCTCCAGA GAGTCAACCG CACCCTCGTT CAGTATAATA AGCCCAGCTC CAGAATGGAG 
GGCGACTGTG CTCTGCTGTC TCTGCCGGTG GCACGGGACA GCCGGGATTC ATGCCCTGAC 
ACGGCTGCTG GCCCCACCAT CGGAAGCGTG GCCAGACCTC CCCGGGACTC CGCAACACCC 
GACCACTGCA CCAGCGGGGC AGAAACGCCC CGGCCTGACC AGCAGCCTCC CACAGGTGGC 
CCCTTGTCCC AGGACCAGCC GCCAAAGACG CCAGAGCAAG AGTGCATGGT GGACTCCCAG 
CCCATCCTCT TCAGTGAGAA CCCCTTTGTG GTGGCCAACC GCAGGGGGAA GGCGGCCGGC 
CGGGCCTGCC TGGGGGGGCC TCCCCTGGGC TATGGCAGGG GGGGAGTGCT GAAGACCAAC 
CTTTACTCCA AGGCTCCTGC AGGCGAGTCT GGGATGGGCA ATTCCAGGCG TGAAACCCCC 
TATTCTCCTG GTAACCAGCA GCGGCCTGCA ACCCCTCGGC CCGCTCACCT GCCGGGCAGG 
GAGACCCGCT TTGCTTCCAT TAACTTCATT ACATCGCAGG ATGACAGTAA ATCGCCAAAG 
CCACCCTCAC CACCCTCTCC TGATGAGGTC CCCATCAATG TCAAGCAAGC GTACAAGACC 
TTCGCTGCAG TGCCCTTGTC ACACCCCCTG CTTGGGACAC AGGAATTGCC TGGTCAGAGC 
GGCACAGAGA ATCCCAGAAG TGCCCCGGAG GTGGCTGCGC ACAGCCCTGG GGGACTGCAC 
AGCCCTGAGC AGAAGTCCTT CCGTGAGAGG CAGAAGTATT TCGAGATTGA GGTGAAGCAG 
CAGCAGATGG ACAAGCCCCC CAAGAGAGTC TCCTTGGTGG GAGAGGACGA CCTGAAGAAG 
ATGAAAGAGG AGGAAGCTCG AAAACTGCAG CAGAAACGTA CCCTTCTGCT GGAGGAAGAG 
GCAGAAGAGG AGGAGATGGC GAAGCAGGCG CCTGAGGTGA GCGTGCAGTC CAGTGTCATC 
ATTGAAGGAG TCGAGTACAA GATTGAGAGA CTCAACGGGA GGAGCAGCCA GTCTCCGGCC 
ACTCGGCCCT CCGAGGTCAA CGAGAACAGC AGCTCGCAGA GGAATTCACT GGAAGAGGGG 
ATCAAGGCTG AGCAGAGAAC CAACTCCATG TCTGGGCTGA GTCCCTTGTA CCTTGGAGCA 
GGGGACGCAG TGGCACCCGT GCGGACGGCC AAAGCTGAAC GGCGACACCA GGAGCGGTTG 
CGAATGCAGA GTCCTGAGCT GCTGAGTGGC CAAGAGAAGG AGCTTTCTCC TGCAGAACGT 
CGTGCTCTGG AGGCGGAGAA ACGAGCAATG TGGAGGGCAG CACGATCCTC AGCTCTTGAA 
GACAGTATTA AACAGTACGA GCAGGACCTG GCCAGGAGGC TGTACCAGTC CCGACAGCGG 
GCACCTGCTC CCGGGGCCGG GCCAGCTCCG ATGCCTAGTC CCAAGCAGAG TCACGCCTCG 
GCACCCCCTG GGTCAGGAAG CCATTCCAGG ATGAAATCGC TTGAACAGGA TGCTCTGAAA 
GCCCAGATGG TCATTGCCAA GTCCAAGGAG GGGAAGAAGC GCAGCCCGCT GGAGCAGCTG 
GCAGAGTCCC CCTCGCCAGT TCCTACACCG TCACCGACAC CACTGGAAGA TTGCAGTCCC 
AGGAACGTCA CCTCCCCAGG AAGGCTGTCC ATGTCTGAAA AGAAGTTTGA CTACCGGGAA 
TTTGCTGCTA TTCCTTCCTC CAAACCCGTT TACGAAATTC AGTCGCCTGA TGCTGCTGAT 
GACCTCAGAT ACATCGAGGA CAGGAATAAC TCAGTTCCCC AGGAGCAGGA TGGGCAGCCC 
GAGGAAGAGG ACACATTAGG AACTCACGAC TCATCCACAC CCACTCCATC AGCACTTGAA 
GAAATGGCCC TGTACAGCAG CAAACGCAAG CTCCGACACA GCCGGCGCAG CCTGGAGGCT 
GCCGTCCCTA CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014801206.1
CDS	6..5840
Translation	MPPLWRCNRHVEALDRRHCSLQAVPEEIYRYSRSLEELLLDANQLRELPKPFFRLLNLRKLGLSDNEIQRLPPEVANFMQLVELDISRNDIPEIPESIKFCKSLEIADFSGNPLSRLPEGFTQLRSLGHLALNDVSLQSLPSDIGNLANLVTLELRENLLKTLPTSLSFLVKLEQLDLGGNDLEVLPDTLGALPNLRELWLDRNQLSALPPELGNLRRLVCLDVSENKLEQLPNEVSGLVALTDLLLSQNLLECIPDGIGQLKQLSILKVDQNRLTEVTESIGDCENLSELILTENMLTALPKSLGKLAKLTNLNVDRNRLTSLPAEIGGCANLNVLSLRDNRLTLLPSELANTTELHVLDVAGNRLQNLPFALTNLNLKALWLAENQSQPMLKFQTEDDEKTGEKVLTCYLLPQQPSPSLENLLQNSVDESWTDTNLNRVSVIQFLDEPKADDEEESGAERRGLQRRATPHPSELKVMKKVIEVRRSEAHAAKPNADLNSPHSEEKRLSATSNRSEDSHLSNSTASADCRAEEQQEEAERSRPNGQVPEMQELEKEAKSRVDEEHKEAAGHEEEDVEVEYNEPTVHFAEDTLIRSEDEDEDEERPFPVEKQRLIRKDTPHYKKHFKITKLPKPEAVVALLQGLNSEGVPKEEEELGGCNNNTEPADQEEVWDEEDRKNGALSAQPSVKGVSFDQANNLLIEPARIEEEELTLTIVRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVRVGDKLLEVNGVSLHSAEHHVAVEALRGSGSSVSMTVLRERMVEPENAITVTPLRPEDDYSPRERRGGLRFPERPEGTPPTERYSTCLMRNEKGLGFSIAGGKGSTPYRAGDMGIFISRIAEGGAAHRDGILHVGDRVISINGVDMTEARHDQAVALLTASSPTIVLLVEREGAEQPGEGDWPGGPRARMHSPPPPPSHGESPPEETPAPQRNHLSKGLEDQYPIEEIHLVKAGGPLGLSIVGGSDHSSHPFGIHEPGVFISKVIPRGLASRSGLRVGDRILEVNSIDLRHATHQEAVNALLSNTQELTMLVRRDPPPPGMQEICIEKAPGEKLGISIRGGAKGHAGNPFDPTDEGIFISKVSSSGAAARDGRLKVGMRILEVNHQSLLGMTHTEAVQMLRGVGDALLVLVCDGFDPKAAAAIEMSPGIIANPFAAGIGRKNSLESISSIDRDLSPEELEILQKEMEMVREATQWEREEMEKVERMRREREAATRQLEEEAENVTSEPLRLDYRTLAALPSSGLQRVNRTLVQYNKPSSRMEGDCALLSLPVARDSRDSCPDTAAGPTIGSVARPPRDSATPDHCTSGAETPRPDQQPPTGGPLSQDQPPKTPEQECMVDSQPILFSENPFVVANRRGKAAGRACLGGPPLGYGRGGVLKTNLYSKAPAGESGMGNSRRETPYSPGNQQRPATPRPAHLPGRETRFASINFITSQDDSKSPKPPSPPSPDEVPINVKQAYKTFAAVPLSHPLLGTQELPGQSGTENPRSAPEVAAHSPGGLHSPEQKSFRERQKYFEIEVKQQQMDKPPKRVSLVGEDDLKKMKEEEARKLQQKRTLLLEEEAEEEEMAKQAPEVSVQSSVIIEGVEYKIERLNGRSSQSPATRPSEVNENSSSQRNSLEEGIKAEQRTNSMSGLSPLYLGAGDAVAPVRTAKAERRHQERLRMQSPELLSGQEKELSPAERRALEAEKRAMWRAARSSALEDSIKQYEQDLARRLYQSRQRAPAPGAGPAPMPSPKQSHASAPPGSGSHSRMKSLEQDALKAQMVIAKSKEGKKRSPLEQLAESPSPVPTPSPTPLEDCSPRNVTSPGRLSMSEKKFDYREFAAIPSSKPVYEIQSPDAADDLRYIEDRNNSVPQEQDGQPEEEDTLGTHDSSTPTPSALEEMALYSSKRKLRHSRRSLEAAVPT

Target ORF information:

RefSeq Version	XM_014945720.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax scribbled planar cell polarity protein (SCRIB), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014945720.1

ORF Insert Sequence:

ATGCCGCCGC TCTGGCGCTG CAACCGGCAC GTGGAGGCGC TGGACCGGCG GCACTGCTCG 
CTGCAGGCCG TGCCCGAGGA GATCTACCGC TACAGCCGCT CGCTGGAGGA GCTGCTGCTG 
GACGCCAACC AGCTGCGGGA GCTGCCCAAG CCTTTCTTCA GGCTTCTGAA CCTGCGCAAG 
CTGGGCCTGA GTGACAATGA GATCCAAAGA CTTCCCCCTG AGGTGGCCAA CTTCATGCAG 
CTGGTGGAAC TGGACATCTC CCGTAACGAC ATTCCAGAAA TTCCTGAAAG CATCAAATTC 
TGCAAATCCT TGGAAATCGC AGACTTCAGT GGGAATCCTC TCTCCAGGCT TCCCGAGGGC 
TTCACGCAGC TTCGGAGCCT GGGCCATTTG GCCCTGAATG ATGTGTCCCT GCAGAGCCTC 
CCCAGTGACA TTGGGAATTT GGCAAACCTG GTGACTCTGG AGCTACGCGA GAACCTGCTA 
AAGACTCTCC CCACCTCGCT GTCCTTCCTT GTCAAGTTAG AGCAGCTGGA TCTCGGTGGC 
AATGACCTGG AGGTTCTGCC AGACACTCTG GGAGCGCTCC CCAACCTGCG TGAGCTCTGG 
TTAGACCGGA ACCAGCTCTC AGCCCTGCCT CCCGAGCTGG GCAACCTCCG CCGCCTGGTC 
TGCCTGGATG TGTCGGAGAA CAAGCTGGAG CAGTTGCCCA ACGAGGTCAG CGGCCTGGTG 
GCACTGACCG ACTTGCTGCT GTCGCAGAAC CTGTTGGAGT GCATTCCTGA TGGGATTGGT 
CAGCTGAAGC AGCTCTCCAT CCTCAAGGTG GACCAGAATC GACTGACAGA GGTGACAGAG 
TCCATTGGGG ACTGTGAAAA TCTGTCCGAG CTCATCCTGA CAGAGAACAT GCTGACGGCC 
TTGCCGAAAT CCCTTGGGAA GCTGGCCAAG CTGACCAACC TGAACGTGGA TCGGAACCGG 
CTGACATCCC TCCCGGCCGA GATCGGTGGC TGTGCCAACC TCAACGTGCT GTCCTTGCGG 
GACAATCGCC TGACCCTCCT GCCATCGGAG CTGGCCAACA CCACCGAGCT TCATGTTCTG 
GATGTGGCTG GGAACAGGCT GCAGAACTTG CCGTTTGCTC TGACAAACCT CAACCTGAAA 
GCCCTGTGGC TGGCGGAGAA TCAGTCCCAG CCCATGCTGA AATTCCAAAC AGAGGATGAT 
GAGAAGACAG GAGAGAAAGT TCTCACTTGT TACCTGCTTC CTCAGCAGCC CTCTCCCAGC 
CTGGAGAACC TTCTACAGAA CAGCGTGGAC GAGAGCTGGA CGGACACCAA TTTAAACAGA 
GTCAGTGTGA TTCAGTTCCT GGATGAACCC AAAGCAGATG ATGAAGAAGA GTCTGGAGCT 
GAGAGAAGGG GCTTGCAGCG CAGAGCAACC CCCCATCCCA GCGAGCTGAA GGTGATGAAG 
AAGGTGATTG AAGTTCGGCG CAGTGAGGCC CACGCTGCAA AGCCCAATGC AGACCTGAAT 
TCTCCTCACT CAGAAGAGAA GAGGCTGAGC GCCACGTCCA ACCGCAGCGA GGACTCCCAC 
CTTTCCAACA GCACTGCCTC TGCTGACTGT AGGGCGGAGG AGCAGCAGGA GGAGGCAGAG 
AGGAGTCGGC CAAACGGGCA AGTGCCTGAG ATGCAGGAGC TGGAGAAGGA GGCAAAGTCC 
AGAGTGGACG AGGAGCACAA GGAAGCTGCT GGCCATGAAG AGGAAGATGT GGAAGTGGAA 
TACAATGAGC CCACTGTACA CTTTGCTGAA GACACGCTAA TACGGAGTGA GGATGAAGAT 
GAAGATGAAG AGCGACCGTT CCCAGTGGAG AAGCAGCGGC TCATCCGCAA GGACACCCCT 
CACTATAAGA AACACTTTAA GATAACTAAA TTGCCCAAGC CAGAGGCGGT GGTGGCCCTG 
CTGCAGGGGC TGAACAGCGA GGGGGTCCCC AAGGAGGAAG AGGAGTTGGG AGGCTGCAAC 
AACAACACAG AGCCTGCAGA CCAGGAGGAG GTGTGGGATG AGGAGGACCG GAAGAATGGA 
GCTTTGTCTG CCCAGCCATC GGTGAAGGGG GTGTCGTTTG ATCAAGCCAA TAATCTGCTG 
ATTGAACCTG CTCGCATTGA GGAGGAAGAG TTGACTCTGA CTATCGTACG GCAGACGGGG 
GGGCTGGGCA TCAGCATCGC AGGGGGCAAG GGCTCCACCC CCTATAAGGG CGATGATGAG 
GGCATCTTTA TTTCTCGGGT GTCAGAAGAA GGTCCTGCGG CTCGTGCAGG GGTTCGTGTT 
GGGGACAAGC TCCTGGAGGT GAACGGTGTG TCCCTGCACT CCGCTGAGCA CCACGTGGCA 
GTGGAGGCTC TGCGTGGATC GGGAAGTTCC GTCTCCATGA CTGTTCTGCG GGAGCGGATG 
GTGGAGCCGG AGAATGCCAT CACTGTCACC CCGCTGCGCC CCGAGGATGA CTACAGCCCC 
CGTGAGAGGC GAGGTGGCCT GCGCTTCCCG GAGCGACCCG AGGGGACACC TCCCACCGAG 
AGATACTCCA CGTGCCTCAT GCGCAACGAG AAGGGACTGG GCTTCAGTAT CGCTGGTGGC 
AAAGGCTCCA CTCCTTACCG GGCTGGTGAC ATGGGGATCT TTATCTCCCG GATCGCAGAG 
GGTGGTGCAG CCCATCGGGA CGGGATCCTG CACGTAGGAG ATCGGGTCAT CTCGATCAAT 
GGGGTAGACA TGACAGAAGC CAGGCATGAT CAGGCAGTAG CGCTGCTTAC CGCGTCCTCC 
CCCACCATCG TGCTGCTGGT AGAGAGAGAG GGTGCGGAGC AGCCCGGCGA GGGAGACTGG 
CCCGGGGGGC CACGGGCACG CATGCACTCC CCACCGCCAC CCCCCAGCCA TGGGGAGAGC 
CCGCCCGAGG AGACGCCCGC ACCACAGAGG AACCATCTCT CCAAAGGCCT GGAGGATCAG 
TACCCCATTG AGGAAATTCA CCTGGTGAAA GCAGGTGGTC CCCTGGGGCT CAGCATCGTA 
GGGGGCAGTG ACCACTCAAG CCATCCGTTT GGGATTCATG AACCAGGTGT CTTCATTTCG 
AAGGTCATTC CCCGTGGGCT GGCATCCCGC AGTGGGCTGC GAGTAGGGGA CAGGATCCTG 
GAAGTCAATA GCATTGACCT GCGCCACGCT ACCCACCAGG AAGCGGTGAA CGCCCTGCTC 
TCCAACACCC AGGAGCTGAC CATGTTAGTG AGGCGAGACC CACCACCACC TGGTATGCAG 
GAAATATGCA TTGAGAAGGC TCCAGGAGAA AAGCTGGGCA TCAGCATCCG GGGTGGAGCG 
AAAGGCCATG CTGGAAATCC GTTTGATCCC ACTGACGAGG GCATCTTCAT CTCTAAGGTG 
AGCTCCTCGG GGGCGGCAGC CCGGGACGGC CGCCTGAAGG TGGGGATGCG GATCCTAGAG 
GTGAACCACC AGAGCCTGCT GGGGATGACG CACACGGAAG CGGTGCAGAT GCTGCGCGGG 
GTGGGCGACG CGCTCCTGGT GCTGGTGTGT GACGGCTTCG ACCCCAAGGC TGCAGCTGCC 
ATCGAGATGT CCCCGGGAAT TATTGCAAAC CCTTTCGCTG CTGGTATCGG ACGCAAGAAT 
AGCCTGGAAA GTATCTCTTC CATCGACCGG GATTTAAGCC CTGAGGAACT GGAGATCCTG 
CAGAAGGAGA TGGAAATGGT GAGAGAAGCA ACTCAGTGGG AGAGAGAAGA AATGGAGAAA 
GTGGAGCGGA TGCGTAGGGA GCGGGAGGCA GCCACCCGGC AGCTTGAGGA GGAGGCGGAG 
AACGTCACCA GCGAGCCCTT GAGACTGGAC TATCGGACCC TGGCTGCGCT GCCGAGCAGT 
GGTCTCCAGA GAGTCAACCG CACCCTCGTT CAGTATAATA AGCCCAGCTC CAGAATGGAG 
GGCGACTGTG CTCTGCTGTC TCTGCCGGTG GCACGGGACA GCCGGGATTC ATGCCCTGAC 
ACGGCTGCTG GCCCCACCAT CGGAAGCGTG GCCAGACCTC CCCGGGACTC CGCAACACCC 
GACCACTGCA CCAGCGGGGC AGAAACGCCC CGGCCTGACC AGCAGCCTCC CACAGGTGGC 
CCCTTGTCCC AGGACCAGCC GCCAAAGACG CCAGAGCAAG AGTGCATGGT GGACTCCCAG 
CCCATCCTCT TCAGTGAGAA CCCCTTTGTG GTGGCCAACC GCAGGGGGAA GGCGGCCGGC 
CGGGCCTGCC TGGGGGGGCC TCCCCTGGGC TATGGCAGGG GGGGAGTGCT GAAGACCAAC 
CTTTACTCCA AGGCTCCTGC AGGCGAGTCT GGGATGGGCA ATTCCAGGCG TGAAACCCCC 
TATTCTCCTG GTAACCAGCA GCGGCCTGCA ACCCCTCGGC CCGCTCACCT GCCGGGCAGG 
GAGACCCGCT TTGCTTCCAT TAACTTCATT ACATCGCAGG ATGACAGTAA ATCGCCAAAG 
CCACCCTCAC CACCCTCTCC TGATGAGGTC CCCATCAATG TCAAGCAAGC GTACAAGACC 
TTCGCTGCAG TGCCCTTGTC ACACCCCCTG CTTGGGACAC AGGAATTGCC TGGTCAGAGC 
GGCACAGAGA ATCCCAGAAG TGCCCCGGAG GTGGCTGCGC ACAGCCCTGG GGGACTGCAC 
AGCCCTGAGC AGAAGTCCTT CCGTGAGAGG CAGAAGTATT TCGAGATTGA GGTGAAGCAG 
CAGCAGATGG ACAAGCCCCC CAAGAGAGTC TCCTTGGTGG GAGAGGACGA CCTGAAGAAG 
ATGAAAGAGG AGGAAGCTCG AAAACTGCAG CAGAAACGTA CCCTTCTGCT GGAGGAAGAG 
GCAGAAGAGG AGGAGATGGC GAAGCAGGCG CCTGAGGTGA GCGTGCAGTC CAGTGTCATC 
ATTGAAGGAG TCGAGTACAA GATTGAGAGA CTCAACGGGA GGAGCAGCCA GTCTCCGGCC 
ACTCGGCCCT CCGAGGTCAA CGAGAACAGC AGCTCGCAGA GGAATTCACT GGAAGAGGGG 
ATCAAGGCTG AGCAGAGAAC CAACTCCATG TCTGGGCTGA GTCCCTTGTA CCTTGGAGCA 
GGGGACGCAG TGGCACCCGT GCGGACGGCC AAAGCTGAAC GGCGACACCA GGAGCGGTTG 
CGAATGCAGA GTCCTGAGCT GCTGAGTGGC CAAGAGAAGG AGCTTTCTCC TGCAGAACGT 
CGTGCTCTGG AGGCGGAGAA ACGAGCAATG TGGAGGGCAG CACGATCCTC AGCTCTTGAA 
GACAGTATTA AACAGTACGA GCAGGACCTG GCCAGGAGGC TGTACCAGTC CCGACAGCGG 
GCACCTGCTC CCGGGGCCGG GCCAGCTCCG ATGCCTAGTC CCAAGCAGAG TCACGCCTCG 
GCACCCCCTG GGTCAGGAAG CCATTCCAGG ATGAAATCGC TTGAACAGGA TGCTCTGAAA 
GCCCAGATGG TCATTGCCAA GTCCAAGGAG GGGAAGAAGC GCAGCCCGCT GGAGCAGCTG 
GCAGAGTCCC CCTCGCCAGT TCCTACACCG TCACCGACAC CACTGGAAGA TTGCAGTCCC 
AGGAACGTCA CCTCCCCAGG AAGGCTGTCC ATGTCTGAAA AGAAGTTTGA CTACCGGGAA 
TTTGCTGCTA TTCCTTCCTC CAAACCCGTT TACGAAATTC AGTCGCCTGA TGCTGCTGAT 
GACCTCAGAT ACATCGAGGA CAGGAATAAC TCAGTTCCCC AGGAGCAGGA TGGGCAGCCC 
GAGGAAGAGG ACACATTAGG AACTCACGAC TCATCCACAC CCACTCCATC AGCACTTGAA 
GAAATGGCCC TGTACAGCAG CAAACGCAAG CTCCGACACA GCCGGCGCAG CCTGGAGGCT 
GCCGTCCCTA CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCRIB ( XM_014945721.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014801207.1 Calidris pugnax scribbled planar cell polarity protein (SCRIB), transcript variant X8, mRNA.

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CloneID	OCa67754
Clone ID Related Accession (Same CDS sequence)	XM_014945721.1
Accession Version	XM_014945721.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5805bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	protein scribble homolog isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090871.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCCGC TCTGGCGCTG CAACCGGCAC GTGGAGGCGC TGGACCGGCG GCACTGCTCG 
CTGCAGGCCG TGCCCGAGGA GATCTACCGC TACAGCCGCT CGCTGGAGGA GCTGCTGCTG 
GACGCCAACC AGCTGCGGGA GCTGCCCAAG CCTTTCTTCA GGCTTCTGAA CCTGCGCAAG 
CTGGGCCTGA GTGACAATGA GATCCAAAGA CTTCCCCCTG AGGTGGCCAA CTTCATGCAG 
CTGGTGGAAC TGGACATCTC CCGTAACGAC ATTCCAGAAA TTCCTGAAAG CATCAAATTC 
TGCAAATCCT TGGAAATCGC AGACTTCAGT GGGAATCCTC TCTCCAGGCT TCCCGAGGGC 
TTCACGCAGC TTCGGAGCCT GGGCCATTTG GCCCTGAATG ATGTGTCCCT GCAGAGCCTC 
CCCAGTGACA TTGGGAATTT GGCAAACCTG GTGACTCTGG AGCTACGCGA GAACCTGCTA 
AAGACTCTCC CCACCTCGCT GTCCTTCCTT GTCAAGTTAG AGCAGCTGGA TCTCGGTGGC 
AATGACCTGG AGGTTCTGCC AGACACTCTG GGAGCGCTCC CCAACCTGCG TGAGCTCTGG 
TTAGACCGGA ACCAGCTCTC AGCCCTGCCT CCCGAGCTGG GCAACCTCCG CCGCCTGGTC 
TGCCTGGATG TGTCGGAGAA CAAGCTGGAG CAGTTGCCCA ACGAGGTCAG CGGCCTGGTG 
GCACTGACCG ACTTGCTGCT GTCGCAGAAC CTGTTGGAGT GCATTCCTGA TGGGATTGGT 
CAGCTGAAGC AGCTCTCCAT CCTCAAGGTG GACCAGAATC GACTGACAGA GGTGACAGAG 
TCCATTGGGG ACTGTGAAAA TCTGTCCGAG CTCATCCTGA CAGAGAACAT GCTGACGGCC 
TTGCCGAAAT CCCTTGGGAA GCTGGCCAAG CTGACCAACC TGAACGTGGA TCGGAACCGG 
CTGACATCCC TCCCGGCCGA GATCGGTGGC TGTGCCAACC TCAACGTGCT GTCCTTGCGG 
GACAATCGCC TGACCCTCCT GCCATCGGAG CTGGCCAACA CCACCGAGCT TCATGTTCTG 
GATGTGGCTG GGAACAGGCT GCAGAACTTG CCGTTTGCTC TGACAAACCT CAACCTGAAA 
GCCCTGTGGC TGGCGGAGAA TCAGTCCCAG CCCATGCTGA AATTCCAAAC AGAGGATGAT 
GAGAAGACAG GAGAGAAAGT TCTCACTTGT TACCTGCTTC CTCAGCAGCC CTCTCCCAGC 
CTGGAGAACC TTCTACAGAA CAGCGTGGAC GAGAGCTGGA CGGACACCAA TTTAAACAGA 
GTCAGTGTGA TTCAGTTCCT GGATGAACCC AAAGCAGATG ATGAAGAAGA GTCTGGAGCT 
GAGAGAAGGG GCTTGCAGCG CAGAGCAACC CCCCATCCCA GCGAGCTGAA GGTGATGAAG 
AAGGTGATTG AAGTTCGGCG CAGTGAGGCC CACGCTGCAA AGCCCAATGC AGACCTGAAT 
TCTCCTCACT CAGAAGAGAA GAGGCTGAGC GCCACGTCCA ACCGCAGCGA GGACTCCCAC 
CTTTCCAACA GCACTGCCTC TGCTGACTGT AGGGCGGAGG AGCAGCAGGA GGAGGCAGAG 
AGGAGTCGGC CAAACGGGCA AGTGCCTGAG ATGCAGGAGC TGGAGAAGGA GGCAAAGTCC 
AGAGTGGACG AGGAGCACAA GGAAGCTGCT GGCCATGAAG AGGAAGATGT GGAAGTGGAA 
TACAATGAGC CCACTGTACA CTTTGCTGAA GACACGCTAA TACGGAGTGA GGATGAAGAT 
GAAGATGAAG AGCGACCGTT CCCAGTGGAG AAGCAGCGGC TCATCCGCAA GGACACCCCT 
CACTATAAGA AACACTTTAA GATAACTAAA TTGCCCAAGC CAGAGGCGGT GGTGGCCCTG 
CTGCAGGGGC TGAACAGCGA GGGGGTCCCC AAGGAGGAAG AGGAGTTGGG AGGCTGCAAC 
AACAACACAG AGCCTGCAGA CCAGGAGGAG GTGTGGGATG AGGAGGACCG GAAGAATGGA 
GCTTTGTCTG CCCAGCCATC GGTGAAGGGG GTGTCGTTTG ATCAAGCCAA TAATCTGCTG 
ATTGAACCTG CTCGCATTGA GGAGGAAGAG TTGACTCTGA CTATCGTACG GCAGACGGGG 
GGGCTGGGCA TCAGCATCGC AGGGGGCAAG GGCTCCACCC CCTATAAGGG CGATGATGAG 
GGCATCTTTA TTTCTCGGGT GTCAGAAGAA GGTCCTGCGG CTCGTGCAGG GGTTCGTGTT 
GGGGACAAGC TCCTGGAGGT GAACGGTGTG TCCCTGCACT CCGCTGAGCA CCACGTGGCA 
GTGGAGGCTC TGCGTGGATC GGGAAGTTCC GTCTCCATGA CTGTTCTGCG GGAGCGGATG 
GTGGAGCCGG AGAATGCCAT CACTGTCACC CCGCTGCGCC CCGAGGATGA CTACAGCCCC 
CGTGAGAGGC GAGGTGGCCT GCGCTTCCCG GAGCGACCCG AGGGGACACC TCCCACCGAG 
AGATACTCCA CGTGCCTCAT GCGCAACGAG AAGGGACTGG GCTTCAGTAT CGCTGGTGGC 
AAAGGCTCCA CTCCTTACCG GGCTGGTGAC ATGGGGATCT TTATCTCCCG GATCGCAGAG 
GGTGGTGCAG CCCATCGGGA CGGGATCCTG CACGTAGGAG ATCGGGTCAT CTCGATCAAT 
GGGGTAGACA TGACAGAAGC CAGGCATGAT CAGGCAGTAG CGCTGCTTAC CGCGTCCTCC 
CCCACCATCG TGCTGCTGGT AGAGAGAGAG GGTGCGGAGC AGCCCGGCGA GGGAGACTGG 
CCCGGGGGGC CACGGGCACG CATGCACTCC CCACCGCCAC CCCCCAGCCA TGGGGAGAGC 
CCGCCCGAGG AGACGCCCGC ACCACAGAGG AACCATCTCT CCAAAGGCCT GGAGGATCAG 
TACCCCATTG AGGAAATTCA CCTGGTGAAA GCAGGTGGTC CCCTGGGGCT CAGCATCGTA 
GGGGGCAGTG ACCACTCAAG CCATCCGTTT GGGATTCATG AACCAGGTGT CTTCATTTCG 
AAGGTCATTC CCCGTGGGCT GGCATCCCGC AGTGGGCTGC GAGTAGGGGA CAGGATCCTG 
GAAGTCAATA GCATTGACCT GCGCCACGCT ACCCACCAGG AAGCGGTGAA CGCCCTGCTC 
TCCAACACCC AGGAGCTGAC CATGTTAGTG AGGCGAGACC CACCACCACC TGGTATGCAG 
GAAATATGCA TTGAGAAGGC TCCAGGAGAA AAGCTGGGCA TCAGCATCCG GGGTGGAGCG 
AAAGGCCATG CTGGAAATCC GTTTGATCCC ACTGACGAGG GCATCTTCAT CTCTAAGGTG 
AGCTCCTCGG GGGCGGCAGC CCGGGACGGC CGCCTGAAGG TGGGGATGCG GATCCTAGAG 
GTGAACCACC AGAGCCTGCT GGGGATGACG CACACGGAAG CGGTGCAGAT GCTGCGCGGG 
GTGGGCGACG CGCTCCTGGT GCTGGTGTGT GACGGCTTCG ACCCCAAGGC TGCAGCTGCC 
ATCGAGATGT CCCCGGGAAT TATTGCAAAC CCTTTCGCTG CTGGTATCGG ACGCAAGAAT 
AGCCTGGAAA GTATCTCTTC CATCGACCGG GATTTAAGCC CTGAGGAACT GGAGATCCTG 
CAGAAGGAGA TGGAAATGGT GAGAGAAGCA ACTCAGTGGG AGAGAGAAGA AATGGAGAAA 
GTGGAGCGGA TGCGTAGGGA GCGGGAGGCA GCCACCCGGC AGCTTGAGGA GGAGGCGGAG 
AACGTCACCA GCGAGCCCTT GAGACTGGAC TATCGGACCC TGGCTGCGCT GCCGAGCAGT 
GGTCTCCAGA GAGTCAACCG CACCCTCGTT CAGTATAATA AGCCCAGCTC CAGAATGGAG 
GGCGACTGTG CTCTGCTGTC TCTGCCGGTG GCACGGGACA GCCGGGATTC ATGCCCTGAC 
ACGGCTGCTG GCCCCACCAT CGGAAGCGTG GCCAGACCTC CCCGGGACTC CGCAACACCC 
GACCACTGCA CCAGCGGGGC AGAAACGCCC CGGCCTGACC AGCAGCCTCC CACAGGTGGC 
CCCTTGTCCC AGGACCAGCC GCCAAAGACG CCAGAGCAAG AGTGCATGGT GGACTCCCAG 
CCCATCCTCT TCAGTGAGAA CCCCTTTGTG GTGGCCAACC GCAGGGGGAA GGCGGCCGGC 
CGGGCCTGCC TGGGGGGGCC TCCCCTGGGC TATGGCAGGG GGGGAGTGCT GAAGACCAAC 
CTTTACTCCA AGGCTCCTGC AGGCGAGTCT GGGATGGGCA ATTCCAGGCG TGAAACCCCC 
TATTCTCCTG GTAACCAGCA GCGGCCTGCA ACCCCTCGGC CCGCTCACCT GCCGGGCAGG 
GAGACCCGCT TTGCTTCCAT TAACTTCATT ACATCGCAGG ATGACAGTAA ATCGACCAAG 
CCAGGCGTCA TTCAGCCTCT TTCCCGAGTG CGACAAAATG CTGCCCCGCA CAATTCGGAG 
GAGGGGGACA GCCCCAATCC CTTCCAGCAG CCCGAGCCGT GCTTCAGTGT CCAGAACTCT 
CCAAAGCCAC CCTCACCACC CTCTCCTGAT GAGGTCCCCA TCAATGTCAA GCAAGCGTAC 
AAGACCTTCG CTGCAGTGCC CTTGTCACAC CCCCTGCTTG GGACACAGGA ATTGCCTGGT 
CAGAGCGGCA CAGAGAATCC CAGAAGTGCC CCGGAGGTGG CTGCGCACAG CCCTGGGGGA 
CTGCACAGCC CTGAGCAGAA GTCCTTCCGT GAGAGGCAGA AGTATTTCGA GATTGAGGTG 
AAGCAGCAGC AGATGGACAA GCCCCCCAAG AGAGTCTCCT TGGTGGGAGA GGACGACCTG 
AAGAAGATGA AAGAGGAGGA AGCTCGAAAA CTGCAGCAGA AACGTACCCT TCTGCTGGAG 
GAAGAGGCAG AAGAGGAGGA GATGGCGAAG CAGGCGCCTG AGGTGAGCGT GCAGTCCAGT 
GTCATCATTG AAGGAGTCGA GTACAAGATT GAGAGACTCA ACGGGAGGAG CAGCCAGTCT 
CCGGCCACTC GGCCCTCCGA GGTCAACGAG AACAGCAGCT CGCAGAGGAA TTCACTGGAA 
GAGGGGATCA AGGCTGAGCA GAGAACCAAC TCCATGTCTG GGCTGAGTCC CTTGTACCTT 
GGAGCAGGGG ACGCAGTGGC ACCCGTGCGG ACGGCCAAAG CTGAACGGCG ACACCAGGAG 
CGGTTGCGAA TGCAGAGTCC TGAGCTGCTG AGTGGCCAAG AGAAGGAGCT TTCTCCTGCA 
GAACGTCGTG CTCTGGAGGC GGAGAAACGA GCAATGTGGA GGGCAGCACG ATCCTCAGCT 
CTTGAAGACA GTATTAAACA GTACGAGCAG GACCTGGCCA GGAGGCTGTA CCAGTCCCGA 
CAGCGGGCAC CTGCTCCCGG GGCCGGGCCA GCTCCGATGC CTAGTCCCAA GCAGAGTCAC 
GCCTCGGCAC CCCCTGGGTC AGGAAGCCAT TCCAGGATGA AATCGCTTGA ACAGGATGCT 
CTGAAAGCCC AGATGGTCAT TGCCAAGTCC AAGGAGGGGA AGAAGCGCAG CCCGCTGGAG 
CAGCTGGCAG AGTCCCCCTC GCCAGTTCCT ACACCGTCAC CGACACCACT GGAAGATTGC 
AGTCCCAGGA ACGTCACCTC CCCAGGAAGG CTGTCCATGT CTGAAAAGAA GTTTGACTAC 
CGGGAATTTG CTGCTATTCC TTCCTCCAAA CCCGTTTACG AAATTCAGTC GCCTGATGCT 
GCTGATGACC TCAGATACAT CGAGGACAGG AATAACTCAG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014801207.1
CDS	6..5810
Translation	MPPLWRCNRHVEALDRRHCSLQAVPEEIYRYSRSLEELLLDANQLRELPKPFFRLLNLRKLGLSDNEIQRLPPEVANFMQLVELDISRNDIPEIPESIKFCKSLEIADFSGNPLSRLPEGFTQLRSLGHLALNDVSLQSLPSDIGNLANLVTLELRENLLKTLPTSLSFLVKLEQLDLGGNDLEVLPDTLGALPNLRELWLDRNQLSALPPELGNLRRLVCLDVSENKLEQLPNEVSGLVALTDLLLSQNLLECIPDGIGQLKQLSILKVDQNRLTEVTESIGDCENLSELILTENMLTALPKSLGKLAKLTNLNVDRNRLTSLPAEIGGCANLNVLSLRDNRLTLLPSELANTTELHVLDVAGNRLQNLPFALTNLNLKALWLAENQSQPMLKFQTEDDEKTGEKVLTCYLLPQQPSPSLENLLQNSVDESWTDTNLNRVSVIQFLDEPKADDEEESGAERRGLQRRATPHPSELKVMKKVIEVRRSEAHAAKPNADLNSPHSEEKRLSATSNRSEDSHLSNSTASADCRAEEQQEEAERSRPNGQVPEMQELEKEAKSRVDEEHKEAAGHEEEDVEVEYNEPTVHFAEDTLIRSEDEDEDEERPFPVEKQRLIRKDTPHYKKHFKITKLPKPEAVVALLQGLNSEGVPKEEEELGGCNNNTEPADQEEVWDEEDRKNGALSAQPSVKGVSFDQANNLLIEPARIEEEELTLTIVRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVRVGDKLLEVNGVSLHSAEHHVAVEALRGSGSSVSMTVLRERMVEPENAITVTPLRPEDDYSPRERRGGLRFPERPEGTPPTERYSTCLMRNEKGLGFSIAGGKGSTPYRAGDMGIFISRIAEGGAAHRDGILHVGDRVISINGVDMTEARHDQAVALLTASSPTIVLLVEREGAEQPGEGDWPGGPRARMHSPPPPPSHGESPPEETPAPQRNHLSKGLEDQYPIEEIHLVKAGGPLGLSIVGGSDHSSHPFGIHEPGVFISKVIPRGLASRSGLRVGDRILEVNSIDLRHATHQEAVNALLSNTQELTMLVRRDPPPPGMQEICIEKAPGEKLGISIRGGAKGHAGNPFDPTDEGIFISKVSSSGAAARDGRLKVGMRILEVNHQSLLGMTHTEAVQMLRGVGDALLVLVCDGFDPKAAAAIEMSPGIIANPFAAGIGRKNSLESISSIDRDLSPEELEILQKEMEMVREATQWEREEMEKVERMRREREAATRQLEEEAENVTSEPLRLDYRTLAALPSSGLQRVNRTLVQYNKPSSRMEGDCALLSLPVARDSRDSCPDTAAGPTIGSVARPPRDSATPDHCTSGAETPRPDQQPPTGGPLSQDQPPKTPEQECMVDSQPILFSENPFVVANRRGKAAGRACLGGPPLGYGRGGVLKTNLYSKAPAGESGMGNSRRETPYSPGNQQRPATPRPAHLPGRETRFASINFITSQDDSKSTKPGVIQPLSRVRQNAAPHNSEEGDSPNPFQQPEPCFSVQNSPKPPSPPSPDEVPINVKQAYKTFAAVPLSHPLLGTQELPGQSGTENPRSAPEVAAHSPGGLHSPEQKSFRERQKYFEIEVKQQQMDKPPKRVSLVGEDDLKKMKEEEARKLQQKRTLLLEEEAEEEEMAKQAPEVSVQSSVIIEGVEYKIERLNGRSSQSPATRPSEVNENSSSQRNSLEEGIKAEQRTNSMSGLSPLYLGAGDAVAPVRTAKAERRHQERLRMQSPELLSGQEKELSPAERRALEAEKRAMWRAARSSALEDSIKQYEQDLARRLYQSRQRAPAPGAGPAPMPSPKQSHASAPPGSGSHSRMKSLEQDALKAQMVIAKSKEGKKRSPLEQLAESPSPVPTPSPTPLEDCSPRNVTSPGRLSMSEKKFDYREFAAIPSSKPVYEIQSPDAADDLRYIEDRNNSE

Target ORF information:

RefSeq Version	XM_014945721.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax scribbled planar cell polarity protein (SCRIB), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014945721.1

ORF Insert Sequence:

ATGCCGCCGC TCTGGCGCTG CAACCGGCAC GTGGAGGCGC TGGACCGGCG GCACTGCTCG 
CTGCAGGCCG TGCCCGAGGA GATCTACCGC TACAGCCGCT CGCTGGAGGA GCTGCTGCTG 
GACGCCAACC AGCTGCGGGA GCTGCCCAAG CCTTTCTTCA GGCTTCTGAA CCTGCGCAAG 
CTGGGCCTGA GTGACAATGA GATCCAAAGA CTTCCCCCTG AGGTGGCCAA CTTCATGCAG 
CTGGTGGAAC TGGACATCTC CCGTAACGAC ATTCCAGAAA TTCCTGAAAG CATCAAATTC 
TGCAAATCCT TGGAAATCGC AGACTTCAGT GGGAATCCTC TCTCCAGGCT TCCCGAGGGC 
TTCACGCAGC TTCGGAGCCT GGGCCATTTG GCCCTGAATG ATGTGTCCCT GCAGAGCCTC 
CCCAGTGACA TTGGGAATTT GGCAAACCTG GTGACTCTGG AGCTACGCGA GAACCTGCTA 
AAGACTCTCC CCACCTCGCT GTCCTTCCTT GTCAAGTTAG AGCAGCTGGA TCTCGGTGGC 
AATGACCTGG AGGTTCTGCC AGACACTCTG GGAGCGCTCC CCAACCTGCG TGAGCTCTGG 
TTAGACCGGA ACCAGCTCTC AGCCCTGCCT CCCGAGCTGG GCAACCTCCG CCGCCTGGTC 
TGCCTGGATG TGTCGGAGAA CAAGCTGGAG CAGTTGCCCA ACGAGGTCAG CGGCCTGGTG 
GCACTGACCG ACTTGCTGCT GTCGCAGAAC CTGTTGGAGT GCATTCCTGA TGGGATTGGT 
CAGCTGAAGC AGCTCTCCAT CCTCAAGGTG GACCAGAATC GACTGACAGA GGTGACAGAG 
TCCATTGGGG ACTGTGAAAA TCTGTCCGAG CTCATCCTGA CAGAGAACAT GCTGACGGCC 
TTGCCGAAAT CCCTTGGGAA GCTGGCCAAG CTGACCAACC TGAACGTGGA TCGGAACCGG 
CTGACATCCC TCCCGGCCGA GATCGGTGGC TGTGCCAACC TCAACGTGCT GTCCTTGCGG 
GACAATCGCC TGACCCTCCT GCCATCGGAG CTGGCCAACA CCACCGAGCT TCATGTTCTG 
GATGTGGCTG GGAACAGGCT GCAGAACTTG CCGTTTGCTC TGACAAACCT CAACCTGAAA 
GCCCTGTGGC TGGCGGAGAA TCAGTCCCAG CCCATGCTGA AATTCCAAAC AGAGGATGAT 
GAGAAGACAG GAGAGAAAGT TCTCACTTGT TACCTGCTTC CTCAGCAGCC CTCTCCCAGC 
CTGGAGAACC TTCTACAGAA CAGCGTGGAC GAGAGCTGGA CGGACACCAA TTTAAACAGA 
GTCAGTGTGA TTCAGTTCCT GGATGAACCC AAAGCAGATG ATGAAGAAGA GTCTGGAGCT 
GAGAGAAGGG GCTTGCAGCG CAGAGCAACC CCCCATCCCA GCGAGCTGAA GGTGATGAAG 
AAGGTGATTG AAGTTCGGCG CAGTGAGGCC CACGCTGCAA AGCCCAATGC AGACCTGAAT 
TCTCCTCACT CAGAAGAGAA GAGGCTGAGC GCCACGTCCA ACCGCAGCGA GGACTCCCAC 
CTTTCCAACA GCACTGCCTC TGCTGACTGT AGGGCGGAGG AGCAGCAGGA GGAGGCAGAG 
AGGAGTCGGC CAAACGGGCA AGTGCCTGAG ATGCAGGAGC TGGAGAAGGA GGCAAAGTCC 
AGAGTGGACG AGGAGCACAA GGAAGCTGCT GGCCATGAAG AGGAAGATGT GGAAGTGGAA 
TACAATGAGC CCACTGTACA CTTTGCTGAA GACACGCTAA TACGGAGTGA GGATGAAGAT 
GAAGATGAAG AGCGACCGTT CCCAGTGGAG AAGCAGCGGC TCATCCGCAA GGACACCCCT 
CACTATAAGA AACACTTTAA GATAACTAAA TTGCCCAAGC CAGAGGCGGT GGTGGCCCTG 
CTGCAGGGGC TGAACAGCGA GGGGGTCCCC AAGGAGGAAG AGGAGTTGGG AGGCTGCAAC 
AACAACACAG AGCCTGCAGA CCAGGAGGAG GTGTGGGATG AGGAGGACCG GAAGAATGGA 
GCTTTGTCTG CCCAGCCATC GGTGAAGGGG GTGTCGTTTG ATCAAGCCAA TAATCTGCTG 
ATTGAACCTG CTCGCATTGA GGAGGAAGAG TTGACTCTGA CTATCGTACG GCAGACGGGG 
GGGCTGGGCA TCAGCATCGC AGGGGGCAAG GGCTCCACCC CCTATAAGGG CGATGATGAG 
GGCATCTTTA TTTCTCGGGT GTCAGAAGAA GGTCCTGCGG CTCGTGCAGG GGTTCGTGTT 
GGGGACAAGC TCCTGGAGGT GAACGGTGTG TCCCTGCACT CCGCTGAGCA CCACGTGGCA 
GTGGAGGCTC TGCGTGGATC GGGAAGTTCC GTCTCCATGA CTGTTCTGCG GGAGCGGATG 
GTGGAGCCGG AGAATGCCAT CACTGTCACC CCGCTGCGCC CCGAGGATGA CTACAGCCCC 
CGTGAGAGGC GAGGTGGCCT GCGCTTCCCG GAGCGACCCG AGGGGACACC TCCCACCGAG 
AGATACTCCA CGTGCCTCAT GCGCAACGAG AAGGGACTGG GCTTCAGTAT CGCTGGTGGC 
AAAGGCTCCA CTCCTTACCG GGCTGGTGAC ATGGGGATCT TTATCTCCCG GATCGCAGAG 
GGTGGTGCAG CCCATCGGGA CGGGATCCTG CACGTAGGAG ATCGGGTCAT CTCGATCAAT 
GGGGTAGACA TGACAGAAGC CAGGCATGAT CAGGCAGTAG CGCTGCTTAC CGCGTCCTCC 
CCCACCATCG TGCTGCTGGT AGAGAGAGAG GGTGCGGAGC AGCCCGGCGA GGGAGACTGG 
CCCGGGGGGC CACGGGCACG CATGCACTCC CCACCGCCAC CCCCCAGCCA TGGGGAGAGC 
CCGCCCGAGG AGACGCCCGC ACCACAGAGG AACCATCTCT CCAAAGGCCT GGAGGATCAG 
TACCCCATTG AGGAAATTCA CCTGGTGAAA GCAGGTGGTC CCCTGGGGCT CAGCATCGTA 
GGGGGCAGTG ACCACTCAAG CCATCCGTTT GGGATTCATG AACCAGGTGT CTTCATTTCG 
AAGGTCATTC CCCGTGGGCT GGCATCCCGC AGTGGGCTGC GAGTAGGGGA CAGGATCCTG 
GAAGTCAATA GCATTGACCT GCGCCACGCT ACCCACCAGG AAGCGGTGAA CGCCCTGCTC 
TCCAACACCC AGGAGCTGAC CATGTTAGTG AGGCGAGACC CACCACCACC TGGTATGCAG 
GAAATATGCA TTGAGAAGGC TCCAGGAGAA AAGCTGGGCA TCAGCATCCG GGGTGGAGCG 
AAAGGCCATG CTGGAAATCC GTTTGATCCC ACTGACGAGG GCATCTTCAT CTCTAAGGTG 
AGCTCCTCGG GGGCGGCAGC CCGGGACGGC CGCCTGAAGG TGGGGATGCG GATCCTAGAG 
GTGAACCACC AGAGCCTGCT GGGGATGACG CACACGGAAG CGGTGCAGAT GCTGCGCGGG 
GTGGGCGACG CGCTCCTGGT GCTGGTGTGT GACGGCTTCG ACCCCAAGGC TGCAGCTGCC 
ATCGAGATGT CCCCGGGAAT TATTGCAAAC CCTTTCGCTG CTGGTATCGG ACGCAAGAAT 
AGCCTGGAAA GTATCTCTTC CATCGACCGG GATTTAAGCC CTGAGGAACT GGAGATCCTG 
CAGAAGGAGA TGGAAATGGT GAGAGAAGCA ACTCAGTGGG AGAGAGAAGA AATGGAGAAA 
GTGGAGCGGA TGCGTAGGGA GCGGGAGGCA GCCACCCGGC AGCTTGAGGA GGAGGCGGAG 
AACGTCACCA GCGAGCCCTT GAGACTGGAC TATCGGACCC TGGCTGCGCT GCCGAGCAGT 
GGTCTCCAGA GAGTCAACCG CACCCTCGTT CAGTATAATA AGCCCAGCTC CAGAATGGAG 
GGCGACTGTG CTCTGCTGTC TCTGCCGGTG GCACGGGACA GCCGGGATTC ATGCCCTGAC 
ACGGCTGCTG GCCCCACCAT CGGAAGCGTG GCCAGACCTC CCCGGGACTC CGCAACACCC 
GACCACTGCA CCAGCGGGGC AGAAACGCCC CGGCCTGACC AGCAGCCTCC CACAGGTGGC 
CCCTTGTCCC AGGACCAGCC GCCAAAGACG CCAGAGCAAG AGTGCATGGT GGACTCCCAG 
CCCATCCTCT TCAGTGAGAA CCCCTTTGTG GTGGCCAACC GCAGGGGGAA GGCGGCCGGC 
CGGGCCTGCC TGGGGGGGCC TCCCCTGGGC TATGGCAGGG GGGGAGTGCT GAAGACCAAC 
CTTTACTCCA AGGCTCCTGC AGGCGAGTCT GGGATGGGCA ATTCCAGGCG TGAAACCCCC 
TATTCTCCTG GTAACCAGCA GCGGCCTGCA ACCCCTCGGC CCGCTCACCT GCCGGGCAGG 
GAGACCCGCT TTGCTTCCAT TAACTTCATT ACATCGCAGG ATGACAGTAA ATCGACCAAG 
CCAGGCGTCA TTCAGCCTCT TTCCCGAGTG CGACAAAATG CTGCCCCGCA CAATTCGGAG 
GAGGGGGACA GCCCCAATCC CTTCCAGCAG CCCGAGCCGT GCTTCAGTGT CCAGAACTCT 
CCAAAGCCAC CCTCACCACC CTCTCCTGAT GAGGTCCCCA TCAATGTCAA GCAAGCGTAC 
AAGACCTTCG CTGCAGTGCC CTTGTCACAC CCCCTGCTTG GGACACAGGA ATTGCCTGGT 
CAGAGCGGCA CAGAGAATCC CAGAAGTGCC CCGGAGGTGG CTGCGCACAG CCCTGGGGGA 
CTGCACAGCC CTGAGCAGAA GTCCTTCCGT GAGAGGCAGA AGTATTTCGA GATTGAGGTG 
AAGCAGCAGC AGATGGACAA GCCCCCCAAG AGAGTCTCCT TGGTGGGAGA GGACGACCTG 
AAGAAGATGA AAGAGGAGGA AGCTCGAAAA CTGCAGCAGA AACGTACCCT TCTGCTGGAG 
GAAGAGGCAG AAGAGGAGGA GATGGCGAAG CAGGCGCCTG AGGTGAGCGT GCAGTCCAGT 
GTCATCATTG AAGGAGTCGA GTACAAGATT GAGAGACTCA ACGGGAGGAG CAGCCAGTCT 
CCGGCCACTC GGCCCTCCGA GGTCAACGAG AACAGCAGCT CGCAGAGGAA TTCACTGGAA 
GAGGGGATCA AGGCTGAGCA GAGAACCAAC TCCATGTCTG GGCTGAGTCC CTTGTACCTT 
GGAGCAGGGG ACGCAGTGGC ACCCGTGCGG ACGGCCAAAG CTGAACGGCG ACACCAGGAG 
CGGTTGCGAA TGCAGAGTCC TGAGCTGCTG AGTGGCCAAG AGAAGGAGCT TTCTCCTGCA 
GAACGTCGTG CTCTGGAGGC GGAGAAACGA GCAATGTGGA GGGCAGCACG ATCCTCAGCT 
CTTGAAGACA GTATTAAACA GTACGAGCAG GACCTGGCCA GGAGGCTGTA CCAGTCCCGA 
CAGCGGGCAC CTGCTCCCGG GGCCGGGCCA GCTCCGATGC CTAGTCCCAA GCAGAGTCAC 
GCCTCGGCAC CCCCTGGGTC AGGAAGCCAT TCCAGGATGA AATCGCTTGA ACAGGATGCT 
CTGAAAGCCC AGATGGTCAT TGCCAAGTCC AAGGAGGGGA AGAAGCGCAG CCCGCTGGAG 
CAGCTGGCAG AGTCCCCCTC GCCAGTTCCT ACACCGTCAC CGACACCACT GGAAGATTGC 
AGTCCCAGGA ACGTCACCTC CCCAGGAAGG CTGTCCATGT CTGAAAAGAA GTTTGACTAC 
CGGGAATTTG CTGCTATTCC TTCCTCCAAA CCCGTTTACG AAATTCAGTC GCCTGATGCT 
GCTGATGACC TCAGATACAT CGAGGACAGG AATAACTCAG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCRIB ( XM_014945722.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014801208.1 Calidris pugnax scribbled planar cell polarity protein (SCRIB), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OCa67755
Clone ID Related Accession (Same CDS sequence)	XM_014945722.1
Accession Version	XM_014945722.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5796bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	protein scribble homolog isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090871.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCCGC TCTGGCGCTG CAACCGGCAC GTGGAGGCGC TGGACCGGCG GCACTGCTCG 
CTGCAGGCCG TGCCCGAGGA GATCTACCGC TACAGCCGCT CGCTGGAGGA GCTGCTGCTG 
GACGCCAACC AGCTGCGGGA GCTGCCCAAG CCTTTCTTCA GGCTTCTGAA CCTGCGCAAG 
CTGGGCCTGA GTGACAATGA GATCCAAAGA CTTCCCCCTG AGGTGGCCAA CTTCATGCAG 
CTGGTGGAAC TGGACATCTC CCGTAACGAC ATTCCAGAAA TTCCTGAAAG CATCAAATTC 
TGCAAATCCT TGGAAATCGC AGACTTCAGT GGGAATCCTC TCTCCAGGCT TCCCGAGGGC 
TTCACGCAGC TTCGGAGCCT GGGCCATTTG GCCCTGAATG ATGTGTCCCT GCAGAGCCTC 
CCCAGTGACA TTGGGAATTT GGCAAACCTG GTGACTCTGG AGCTACGCGA GAACCTGCTA 
AAGACTCTCC CCACCTCGCT GTCCTTCCTT GTCAAGTTAG AGCAGCTGGA TCTCGGTGGC 
AATGACCTGG AGGTTCTGCC AGACACTCTG GGAGCGCTCC CCAACCTGCG TGAGCTCTGG 
TTAGACCGGA ACCAGCTCTC AGCCCTGCCT CCCGAGCTGG GCAACCTCCG CCGCCTGGTC 
TGCCTGGATG TGTCGGAGAA CAAGCTGGAG CAGTTGCCCA ACGAGGTCAG CGGCCTGGTG 
GCACTGACCG ACTTGCTGCT GTCGCAGAAC CTGTTGGAGT GCATTCCTGA TGGGATTGGT 
CAGCTGAAGC AGCTCTCCAT CCTCAAGGTG GACCAGAATC GACTGACAGA GGTGACAGAG 
TCCATTGGGG ACTGTGAAAA TCTGTCCGAG CTCATCCTGA CAGAGAACAT GCTGACGGCC 
TTGCCGAAAT CCCTTGGGAA GCTGGCCAAG CTGACCAACC TGAACGTGGA TCGGAACCGG 
CTGACATCCC TCCCGGCCGA GATCGGTGGC TGTGCCAACC TCAACGTGCT GTCCTTGCGG 
GACAATCGCC TGACCCTCCT GCCATCGGAG CTGGCCAACA CCACCGAGCT TCATGTTCTG 
GATGTGGCTG GGAACAGGCT GCAGAACTTG CCGTTTGCTC TGACAAACCT CAACCTGAAA 
GCCCTGTGGC TGGCGGAGAA TCAGTCCCAG CCCATGCTGA AATTCCAAAC AGAGGATGAT 
GAGAAGACAG GAGAGAAAGT TCTCACTTGT TACCTGCTTC CTCAGCAGCC CTCTCCCAGC 
CTGGAGAACC TTCTACAGAA CAGCGTGGAC GAGAGCTGGA CGGACACCAA TTTAAACAGA 
GTCAGTGTGA TTCAGTTCCT GGATGAACCC AAAGCAGATG ATGAAGAAGA GTCTGGAGCT 
GAGAGAAGGG GCTTGCAGCG CAGAGCAACC CCCCATCCCA GCGAGCTGAA GGTGATGAAG 
AAGGTGATTG AAGTTCGGCG CAGTGAGGCC CACGCTGCAA AGCCCAATGC AGACCTGAAT 
TCTCCTCACT CAGAAGAGAA GAGGCTGAGC GCCACGTCCA ACCGCAGCGA GGACTCCCAC 
CTTTCCAACA GCACTGCCTC TGCTGACTGT AGGGCGGAGG AGCAGCAGGA GGAGGCAGAG 
AGGAGTCGGC CAAACGGGCA AGTGCCTGAG ATGCAGGAGC TGGAGAAGGA GGCAAAGTCC 
AGAGTGGACG AGGAGCACAA GGAAGCTGCT GGCCATGAAG AGGAAGATGT GGAAGTGGAA 
TACAATGAGC CCACTGTACA CTTTGCTGAA GACACGCTAA TACGGAGTGA GGATGAAGAT 
GAAGATGAAG AGCGACCGTT CCCAGTGGAG AAGCAGCGGC TCATCCGCAA GGACACCCCT 
CACTATAAGA AACACTTTAA GATAACTAAA TTGCCCAAGC CAGAGGCGGT GGTGGCCCTG 
CTGCAGGGGC TGAACAGCGA GGGGGTCCCC AAGGAGGAAG AGGAGTTGGG AGGCTGCAAC 
AACAACACAG AGCCTGCAGA CCAGGAGGAG GTGTGGGATG AGGAGGACCG GAAGAATGGA 
GCTTTGTCTG CCCAGCCATC GGTGAAGGGG GTGTCGTTTG ATCAAGCCAA TAATCTGCTG 
ATTGAACCTG CTCGCATTGA GGAGGAAGAG TTGACTCTGA CTATCGTACG GCAGACGGGG 
GGGCTGGGCA TCAGCATCGC AGGGGGCAAG GGCTCCACCC CCTATAAGGG CGATGATGAG 
GGCATCTTTA TTTCTCGGGT GTCAGAAGAA GGTCCTGCGG CTCGTGCAGG GGTTCGTGTT 
GGGGACAAGC TCCTGGAGGT GAACGGTGTG TCCCTGCACT CCGCTGAGCA CCACGTGGCA 
GTGGAGGCTC TGCGTGGATC GGGAAGTTCC GTCTCCATGA CTGTTCTGCG GGAGCGGATG 
GTGGAGCCGG AGAATGCCAT CACTGTCACC CCGCTGCGCC CCGAGGATGA CTACAGCCCC 
CGTGAGAGGC GAGGTGGCCT GCGCTTCCCG GAGCGACCCG AGGGGACACC TCCCACCGAG 
AGATACTCCA CGTGCCTCAT GCGCAACGAG AAGGGACTGG GCTTCAGTAT CGCTGGTGGC 
AAAGGCTCCA CTCCTTACCG GGCTGGTGAC ATGGGGATCT TTATCTCCCG GATCGCAGAG 
GGTGGTGCAG CCCATCGGGA CGGGATCCTG CACGTAGGAG ATCGGGTCAT CTCGATCAAT 
GGGGTAGACA TGACAGAAGC CAGGCATGAT CAGGCAGTAG CGCTGCTTAC CGCGTCCTCC 
CCCACCATCG TGCTGCTGGT AGAGAGAGAG GGTGCGGAGC AGCCCGGCGA GGGAGACTGG 
CCCGGGGGGC CACGGGCACG CATGCACTCC CCACCGCCAC CCCCCAGCCA TGGGGAGAGC 
CCGCCCGAGG AGACGCCCGC ACCACAGAGG AACCATCTCT CCAAAGGCCT GGAGGATCAG 
TACCCCATTG AGGAAATTCA CCTGGTGAAA GCAGGTGGTC CCCTGGGGCT CAGCATCGTA 
GGGGGCAGTG ACCACTCAAG CCATCCGTTT GGGATTCATG AACCAGGTGT CTTCATTTCG 
AAGGTCATTC CCCGTGGGCT GGCATCCCGC AGTGGGCTGC GAGTAGGGGA CAGGATCCTG 
GAAGTCAATA GCATTGACCT GCGCCACGCT ACCCACCAGG AAGCGGTGAA CGCCCTGCTC 
TCCAACACCC AGGAGCTGAC CATGTTAGTG AGGCGAGACC CACCACCACC TGGTATGCAG 
GAAATATGCA TTGAGAAGGC TCCAGGAGAA AAGCTGGGCA TCAGCATCCG GGGTGGAGCG 
AAAGGCCATG CTGGAAATCC GTTTGATCCC ACTGACGAGG GCATCTTCAT CTCTAAGGTG 
AGCTCCTCGG GGGCGGCAGC CCGGGACGGC CGCCTGAAGG TGGGGATGCG GATCCTAGAG 
GTGAACCACC AGAGCCTGCT GGGGATGACG CACACGGAAG CGGTGCAGAT GCTGCGCGGG 
GTGGGCGACG CGCTCCTGGT GCTGGTGTGT GACGGCTTCG ACCCCAAGGC TGCAGCTGCC 
ATCGAGATGT CCCCGGGAAT TATTGCAAAC CCTTTCGCTG CTGGTATCGG ACGCAAGAAT 
AGCCTGGAAA GTATCTCTTC CATCGACCGG GATTTAAGCC CTGAGGAACT GGAGATCCTG 
CAGAAGGAGA TGGAAATGGT GAGAGAAGCA ACTCAGTGGG AGAGAGAAGA AATGGAGAAA 
GTGGAGCGGA TGCGTAGGGA GCGGGAGGCA GCCACCCGGC AGCTTGAGGA GGAGGCGGAG 
AACGTCACCA GCGAGCCCTT GAGACTGGAC TATCGGACCC TGGCTGCGCT GCCGAGCAGT 
GGTCTCCAGA GAGTCAACCG CACCCTCGTT CAGTATAATA AGCCCAGCTC CAGAATGGAG 
GGCGACTGTG CTCTGCTGTC TCTGCCGGTG GCACGGGACA GCCGGGATTC ATGCCCTGAC 
ACGGCTGCTG GCCCCACCAT CGGAAGCGTG GCCAGACCTC CCCGGGACTC CGCAACACCC 
GACCACTGCA CCAGCGGGGC AGAAACGCCC CGGCCTGACC AGCAGCCTCC CACAGGTGGC 
CCCTTGTCCC AGGACCAGCC GCCAAAGACG CCAGAGCAAG AGTGCATGGT GGACTCCCAG 
CCCATCCTCT TCAGTGAGAA CCCCTTTGTG GTGGCCAACC GCAGGGGGAA GGCGGCCGGC 
CGGGCCTGCC TGGGGGGGCC TCCCCTGGGC TATGGCAGGG GGGGAGTGCT GAAGACCAAC 
CTTTACTCCA AGGCTCCTGC AGGCGAGTCT GGGATGGGCA ATTCCAGGCG TGAAACCCCC 
TATTCTCCTG GTAACCAGCA GCGGCCTGCA ACCCCTCGGC CCGCTCACCT GCCGGGCAGG 
GAGACCCGCT TTGCTTCCAT TAACTTCATT ACATCGCAGG ATGACAGTAA ATCGACCAAG 
CCAGGCGTCA TTCAGCCTCT TTCCCGAGTG CGACAAAATG CTGCCCCGCA CAATTCGGAG 
GAGGGGGACA GCCCCAATCC CTTCCAGCAG CCCGAGCCGT GCTTCAGTGT CCAGAACTCT 
CCAAAGCCAC CCTCACCACC CTCTCCTGAT GAGGTCCCCA TCAATGTCAA GCAAGCGTAC 
AAGACCTTCG CTGCAGTGCC CTTGTCACAC CCCCTGCTTG GGACACAGGA ATTGCCTGGT 
CAGAGCGGCA CAGAGAATCC CAGAAGTGCC CCGGAGGTGG CTGCGCACAG CCCTGGGGGA 
CTGCACAGCC CTGAGCAGAA GTCCTTCCGT GAGAGGCAGA AGTATTTCGA GATTGAGGTG 
AAGCAGCAGC AGATGGACAA GCCCCCCAAG AGAGTCTCCT TGGTGGGAGA GGACGACCTG 
AAGAAGATGA AAGAGGAGGA AGCTCGAAAA CTGCAGCAGA AACGTACCCT TCTGCTGGAG 
GAAGAGGCAG AAGAGGAGGA GATGGCGAAG CAGGCGCCTG AGGTGAGCGT GCAGTCCAGT 
GTCATCATTG AAGGAGTCGA GTACAAGATT GAGAGACTCA ACGGGAGGAG CAGCCAGTCT 
CCGGCCACTC GGCCCTCCGA GGTCAACGAG AACAGCAGCT CGCAGAGGAA TTCACTGGAA 
GAGGGGATCA AGGCTGAGCA GAGAACCAAC TCCATGTCTG GGCTGAGTCC CTTGTACCTT 
GGAGCAGGGG ACGCAGTGGC ACCCGTGCGG ACGGCCAAAG CTGAACGGCG ACACCAGGAG 
CGGTTGCGAA TGCAGAGTCC TGAGCTGCTG AGTGGCCAAG AGAAGGAGCT TTCTCCTGCA 
GAACGTCGTG CTCTGGAGGC GGAGAAACGA GCAATGTGGA GGGCAGCACG GATGAAATCG 
CTTGAACAGG ATGCTCTGAA AGCCCAGATG GTCATTGCCA AGTCCAAGGA GGGGAAGAAG 
CGCAGCCCGC TGGAGCAGCT GGCAGAGTCC CCCTCGCCAG TTCCTACACC GTCACCGACA 
CCACTGGAAG ATTGCAGTCC CAGGAACGTC ACCTCCCCAG GAAGGCTGTC CATGTCTGAA 
AAGAAGTTTG ACTACCGGGA ATTTGCTGCT ATTCCTTCCT CCAAACCCGT TTACGAAATT 
CAGTCGCCTG ATGCTGCTGA TGACCTCAGA TACATCGAGG ACAGGAATAA CTCAGTTCCC 
CAGGAGCAGG ATGGGCAGCC CGAGGAAGAG GACACATTAG GAACTCACGA CTCATCCACA 
CCCACTCCAT CAGCACTTGA AGAAATGGCC CTGTACAGCA GCAAACGCAA GCTCCGACAC 
AGCCGGCGCA GCCTGGAGGC TGCCGTCCCT ACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014801208.1
CDS	6..5801
Translation	MPPLWRCNRHVEALDRRHCSLQAVPEEIYRYSRSLEELLLDANQLRELPKPFFRLLNLRKLGLSDNEIQRLPPEVANFMQLVELDISRNDIPEIPESIKFCKSLEIADFSGNPLSRLPEGFTQLRSLGHLALNDVSLQSLPSDIGNLANLVTLELRENLLKTLPTSLSFLVKLEQLDLGGNDLEVLPDTLGALPNLRELWLDRNQLSALPPELGNLRRLVCLDVSENKLEQLPNEVSGLVALTDLLLSQNLLECIPDGIGQLKQLSILKVDQNRLTEVTESIGDCENLSELILTENMLTALPKSLGKLAKLTNLNVDRNRLTSLPAEIGGCANLNVLSLRDNRLTLLPSELANTTELHVLDVAGNRLQNLPFALTNLNLKALWLAENQSQPMLKFQTEDDEKTGEKVLTCYLLPQQPSPSLENLLQNSVDESWTDTNLNRVSVIQFLDEPKADDEEESGAERRGLQRRATPHPSELKVMKKVIEVRRSEAHAAKPNADLNSPHSEEKRLSATSNRSEDSHLSNSTASADCRAEEQQEEAERSRPNGQVPEMQELEKEAKSRVDEEHKEAAGHEEEDVEVEYNEPTVHFAEDTLIRSEDEDEDEERPFPVEKQRLIRKDTPHYKKHFKITKLPKPEAVVALLQGLNSEGVPKEEEELGGCNNNTEPADQEEVWDEEDRKNGALSAQPSVKGVSFDQANNLLIEPARIEEEELTLTIVRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVRVGDKLLEVNGVSLHSAEHHVAVEALRGSGSSVSMTVLRERMVEPENAITVTPLRPEDDYSPRERRGGLRFPERPEGTPPTERYSTCLMRNEKGLGFSIAGGKGSTPYRAGDMGIFISRIAEGGAAHRDGILHVGDRVISINGVDMTEARHDQAVALLTASSPTIVLLVEREGAEQPGEGDWPGGPRARMHSPPPPPSHGESPPEETPAPQRNHLSKGLEDQYPIEEIHLVKAGGPLGLSIVGGSDHSSHPFGIHEPGVFISKVIPRGLASRSGLRVGDRILEVNSIDLRHATHQEAVNALLSNTQELTMLVRRDPPPPGMQEICIEKAPGEKLGISIRGGAKGHAGNPFDPTDEGIFISKVSSSGAAARDGRLKVGMRILEVNHQSLLGMTHTEAVQMLRGVGDALLVLVCDGFDPKAAAAIEMSPGIIANPFAAGIGRKNSLESISSIDRDLSPEELEILQKEMEMVREATQWEREEMEKVERMRREREAATRQLEEEAENVTSEPLRLDYRTLAALPSSGLQRVNRTLVQYNKPSSRMEGDCALLSLPVARDSRDSCPDTAAGPTIGSVARPPRDSATPDHCTSGAETPRPDQQPPTGGPLSQDQPPKTPEQECMVDSQPILFSENPFVVANRRGKAAGRACLGGPPLGYGRGGVLKTNLYSKAPAGESGMGNSRRETPYSPGNQQRPATPRPAHLPGRETRFASINFITSQDDSKSTKPGVIQPLSRVRQNAAPHNSEEGDSPNPFQQPEPCFSVQNSPKPPSPPSPDEVPINVKQAYKTFAAVPLSHPLLGTQELPGQSGTENPRSAPEVAAHSPGGLHSPEQKSFRERQKYFEIEVKQQQMDKPPKRVSLVGEDDLKKMKEEEARKLQQKRTLLLEEEAEEEEMAKQAPEVSVQSSVIIEGVEYKIERLNGRSSQSPATRPSEVNENSSSQRNSLEEGIKAEQRTNSMSGLSPLYLGAGDAVAPVRTAKAERRHQERLRMQSPELLSGQEKELSPAERRALEAEKRAMWRAARMKSLEQDALKAQMVIAKSKEGKKRSPLEQLAESPSPVPTPSPTPLEDCSPRNVTSPGRLSMSEKKFDYREFAAIPSSKPVYEIQSPDAADDLRYIEDRNNSVPQEQDGQPEEEDTLGTHDSSTPTPSALEEMALYSSKRKLRHSRRSLEAAVPT

Target ORF information:

RefSeq Version	XM_014945722.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax scribbled planar cell polarity protein (SCRIB), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014945722.1

ORF Insert Sequence:

ATGCCGCCGC TCTGGCGCTG CAACCGGCAC GTGGAGGCGC TGGACCGGCG GCACTGCTCG 
CTGCAGGCCG TGCCCGAGGA GATCTACCGC TACAGCCGCT CGCTGGAGGA GCTGCTGCTG 
GACGCCAACC AGCTGCGGGA GCTGCCCAAG CCTTTCTTCA GGCTTCTGAA CCTGCGCAAG 
CTGGGCCTGA GTGACAATGA GATCCAAAGA CTTCCCCCTG AGGTGGCCAA CTTCATGCAG 
CTGGTGGAAC TGGACATCTC CCGTAACGAC ATTCCAGAAA TTCCTGAAAG CATCAAATTC 
TGCAAATCCT TGGAAATCGC AGACTTCAGT GGGAATCCTC TCTCCAGGCT TCCCGAGGGC 
TTCACGCAGC TTCGGAGCCT GGGCCATTTG GCCCTGAATG ATGTGTCCCT GCAGAGCCTC 
CCCAGTGACA TTGGGAATTT GGCAAACCTG GTGACTCTGG AGCTACGCGA GAACCTGCTA 
AAGACTCTCC CCACCTCGCT GTCCTTCCTT GTCAAGTTAG AGCAGCTGGA TCTCGGTGGC 
AATGACCTGG AGGTTCTGCC AGACACTCTG GGAGCGCTCC CCAACCTGCG TGAGCTCTGG 
TTAGACCGGA ACCAGCTCTC AGCCCTGCCT CCCGAGCTGG GCAACCTCCG CCGCCTGGTC 
TGCCTGGATG TGTCGGAGAA CAAGCTGGAG CAGTTGCCCA ACGAGGTCAG CGGCCTGGTG 
GCACTGACCG ACTTGCTGCT GTCGCAGAAC CTGTTGGAGT GCATTCCTGA TGGGATTGGT 
CAGCTGAAGC AGCTCTCCAT CCTCAAGGTG GACCAGAATC GACTGACAGA GGTGACAGAG 
TCCATTGGGG ACTGTGAAAA TCTGTCCGAG CTCATCCTGA CAGAGAACAT GCTGACGGCC 
TTGCCGAAAT CCCTTGGGAA GCTGGCCAAG CTGACCAACC TGAACGTGGA TCGGAACCGG 
CTGACATCCC TCCCGGCCGA GATCGGTGGC TGTGCCAACC TCAACGTGCT GTCCTTGCGG 
GACAATCGCC TGACCCTCCT GCCATCGGAG CTGGCCAACA CCACCGAGCT TCATGTTCTG 
GATGTGGCTG GGAACAGGCT GCAGAACTTG CCGTTTGCTC TGACAAACCT CAACCTGAAA 
GCCCTGTGGC TGGCGGAGAA TCAGTCCCAG CCCATGCTGA AATTCCAAAC AGAGGATGAT 
GAGAAGACAG GAGAGAAAGT TCTCACTTGT TACCTGCTTC CTCAGCAGCC CTCTCCCAGC 
CTGGAGAACC TTCTACAGAA CAGCGTGGAC GAGAGCTGGA CGGACACCAA TTTAAACAGA 
GTCAGTGTGA TTCAGTTCCT GGATGAACCC AAAGCAGATG ATGAAGAAGA GTCTGGAGCT 
GAGAGAAGGG GCTTGCAGCG CAGAGCAACC CCCCATCCCA GCGAGCTGAA GGTGATGAAG 
AAGGTGATTG AAGTTCGGCG CAGTGAGGCC CACGCTGCAA AGCCCAATGC AGACCTGAAT 
TCTCCTCACT CAGAAGAGAA GAGGCTGAGC GCCACGTCCA ACCGCAGCGA GGACTCCCAC 
CTTTCCAACA GCACTGCCTC TGCTGACTGT AGGGCGGAGG AGCAGCAGGA GGAGGCAGAG 
AGGAGTCGGC CAAACGGGCA AGTGCCTGAG ATGCAGGAGC TGGAGAAGGA GGCAAAGTCC 
AGAGTGGACG AGGAGCACAA GGAAGCTGCT GGCCATGAAG AGGAAGATGT GGAAGTGGAA 
TACAATGAGC CCACTGTACA CTTTGCTGAA GACACGCTAA TACGGAGTGA GGATGAAGAT 
GAAGATGAAG AGCGACCGTT CCCAGTGGAG AAGCAGCGGC TCATCCGCAA GGACACCCCT 
CACTATAAGA AACACTTTAA GATAACTAAA TTGCCCAAGC CAGAGGCGGT GGTGGCCCTG 
CTGCAGGGGC TGAACAGCGA GGGGGTCCCC AAGGAGGAAG AGGAGTTGGG AGGCTGCAAC 
AACAACACAG AGCCTGCAGA CCAGGAGGAG GTGTGGGATG AGGAGGACCG GAAGAATGGA 
GCTTTGTCTG CCCAGCCATC GGTGAAGGGG GTGTCGTTTG ATCAAGCCAA TAATCTGCTG 
ATTGAACCTG CTCGCATTGA GGAGGAAGAG TTGACTCTGA CTATCGTACG GCAGACGGGG 
GGGCTGGGCA TCAGCATCGC AGGGGGCAAG GGCTCCACCC CCTATAAGGG CGATGATGAG 
GGCATCTTTA TTTCTCGGGT GTCAGAAGAA GGTCCTGCGG CTCGTGCAGG GGTTCGTGTT 
GGGGACAAGC TCCTGGAGGT GAACGGTGTG TCCCTGCACT CCGCTGAGCA CCACGTGGCA 
GTGGAGGCTC TGCGTGGATC GGGAAGTTCC GTCTCCATGA CTGTTCTGCG GGAGCGGATG 
GTGGAGCCGG AGAATGCCAT CACTGTCACC CCGCTGCGCC CCGAGGATGA CTACAGCCCC 
CGTGAGAGGC GAGGTGGCCT GCGCTTCCCG GAGCGACCCG AGGGGACACC TCCCACCGAG 
AGATACTCCA CGTGCCTCAT GCGCAACGAG AAGGGACTGG GCTTCAGTAT CGCTGGTGGC 
AAAGGCTCCA CTCCTTACCG GGCTGGTGAC ATGGGGATCT TTATCTCCCG GATCGCAGAG 
GGTGGTGCAG CCCATCGGGA CGGGATCCTG CACGTAGGAG ATCGGGTCAT CTCGATCAAT 
GGGGTAGACA TGACAGAAGC CAGGCATGAT CAGGCAGTAG CGCTGCTTAC CGCGTCCTCC 
CCCACCATCG TGCTGCTGGT AGAGAGAGAG GGTGCGGAGC AGCCCGGCGA GGGAGACTGG 
CCCGGGGGGC CACGGGCACG CATGCACTCC CCACCGCCAC CCCCCAGCCA TGGGGAGAGC 
CCGCCCGAGG AGACGCCCGC ACCACAGAGG AACCATCTCT CCAAAGGCCT GGAGGATCAG 
TACCCCATTG AGGAAATTCA CCTGGTGAAA GCAGGTGGTC CCCTGGGGCT CAGCATCGTA 
GGGGGCAGTG ACCACTCAAG CCATCCGTTT GGGATTCATG AACCAGGTGT CTTCATTTCG 
AAGGTCATTC CCCGTGGGCT GGCATCCCGC AGTGGGCTGC GAGTAGGGGA CAGGATCCTG 
GAAGTCAATA GCATTGACCT GCGCCACGCT ACCCACCAGG AAGCGGTGAA CGCCCTGCTC 
TCCAACACCC AGGAGCTGAC CATGTTAGTG AGGCGAGACC CACCACCACC TGGTATGCAG 
GAAATATGCA TTGAGAAGGC TCCAGGAGAA AAGCTGGGCA TCAGCATCCG GGGTGGAGCG 
AAAGGCCATG CTGGAAATCC GTTTGATCCC ACTGACGAGG GCATCTTCAT CTCTAAGGTG 
AGCTCCTCGG GGGCGGCAGC CCGGGACGGC CGCCTGAAGG TGGGGATGCG GATCCTAGAG 
GTGAACCACC AGAGCCTGCT GGGGATGACG CACACGGAAG CGGTGCAGAT GCTGCGCGGG 
GTGGGCGACG CGCTCCTGGT GCTGGTGTGT GACGGCTTCG ACCCCAAGGC TGCAGCTGCC 
ATCGAGATGT CCCCGGGAAT TATTGCAAAC CCTTTCGCTG CTGGTATCGG ACGCAAGAAT 
AGCCTGGAAA GTATCTCTTC CATCGACCGG GATTTAAGCC CTGAGGAACT GGAGATCCTG 
CAGAAGGAGA TGGAAATGGT GAGAGAAGCA ACTCAGTGGG AGAGAGAAGA AATGGAGAAA 
GTGGAGCGGA TGCGTAGGGA GCGGGAGGCA GCCACCCGGC AGCTTGAGGA GGAGGCGGAG 
AACGTCACCA GCGAGCCCTT GAGACTGGAC TATCGGACCC TGGCTGCGCT GCCGAGCAGT 
GGTCTCCAGA GAGTCAACCG CACCCTCGTT CAGTATAATA AGCCCAGCTC CAGAATGGAG 
GGCGACTGTG CTCTGCTGTC TCTGCCGGTG GCACGGGACA GCCGGGATTC ATGCCCTGAC 
ACGGCTGCTG GCCCCACCAT CGGAAGCGTG GCCAGACCTC CCCGGGACTC CGCAACACCC 
GACCACTGCA CCAGCGGGGC AGAAACGCCC CGGCCTGACC AGCAGCCTCC CACAGGTGGC 
CCCTTGTCCC AGGACCAGCC GCCAAAGACG CCAGAGCAAG AGTGCATGGT GGACTCCCAG 
CCCATCCTCT TCAGTGAGAA CCCCTTTGTG GTGGCCAACC GCAGGGGGAA GGCGGCCGGC 
CGGGCCTGCC TGGGGGGGCC TCCCCTGGGC TATGGCAGGG GGGGAGTGCT GAAGACCAAC 
CTTTACTCCA AGGCTCCTGC AGGCGAGTCT GGGATGGGCA ATTCCAGGCG TGAAACCCCC 
TATTCTCCTG GTAACCAGCA GCGGCCTGCA ACCCCTCGGC CCGCTCACCT GCCGGGCAGG 
GAGACCCGCT TTGCTTCCAT TAACTTCATT ACATCGCAGG ATGACAGTAA ATCGACCAAG 
CCAGGCGTCA TTCAGCCTCT TTCCCGAGTG CGACAAAATG CTGCCCCGCA CAATTCGGAG 
GAGGGGGACA GCCCCAATCC CTTCCAGCAG CCCGAGCCGT GCTTCAGTGT CCAGAACTCT 
CCAAAGCCAC CCTCACCACC CTCTCCTGAT GAGGTCCCCA TCAATGTCAA GCAAGCGTAC 
AAGACCTTCG CTGCAGTGCC CTTGTCACAC CCCCTGCTTG GGACACAGGA ATTGCCTGGT 
CAGAGCGGCA CAGAGAATCC CAGAAGTGCC CCGGAGGTGG CTGCGCACAG CCCTGGGGGA 
CTGCACAGCC CTGAGCAGAA GTCCTTCCGT GAGAGGCAGA AGTATTTCGA GATTGAGGTG 
AAGCAGCAGC AGATGGACAA GCCCCCCAAG AGAGTCTCCT TGGTGGGAGA GGACGACCTG 
AAGAAGATGA AAGAGGAGGA AGCTCGAAAA CTGCAGCAGA AACGTACCCT TCTGCTGGAG 
GAAGAGGCAG AAGAGGAGGA GATGGCGAAG CAGGCGCCTG AGGTGAGCGT GCAGTCCAGT 
GTCATCATTG AAGGAGTCGA GTACAAGATT GAGAGACTCA ACGGGAGGAG CAGCCAGTCT 
CCGGCCACTC GGCCCTCCGA GGTCAACGAG AACAGCAGCT CGCAGAGGAA TTCACTGGAA 
GAGGGGATCA AGGCTGAGCA GAGAACCAAC TCCATGTCTG GGCTGAGTCC CTTGTACCTT 
GGAGCAGGGG ACGCAGTGGC ACCCGTGCGG ACGGCCAAAG CTGAACGGCG ACACCAGGAG 
CGGTTGCGAA TGCAGAGTCC TGAGCTGCTG AGTGGCCAAG AGAAGGAGCT TTCTCCTGCA 
GAACGTCGTG CTCTGGAGGC GGAGAAACGA GCAATGTGGA GGGCAGCACG GATGAAATCG 
CTTGAACAGG ATGCTCTGAA AGCCCAGATG GTCATTGCCA AGTCCAAGGA GGGGAAGAAG 
CGCAGCCCGC TGGAGCAGCT GGCAGAGTCC CCCTCGCCAG TTCCTACACC GTCACCGACA 
CCACTGGAAG ATTGCAGTCC CAGGAACGTC ACCTCCCCAG GAAGGCTGTC CATGTCTGAA 
AAGAAGTTTG ACTACCGGGA ATTTGCTGCT ATTCCTTCCT CCAAACCCGT TTACGAAATT 
CAGTCGCCTG ATGCTGCTGA TGACCTCAGA TACATCGAGG ACAGGAATAA CTCAGTTCCC 
CAGGAGCAGG ATGGGCAGCC CGAGGAAGAG GACACATTAG GAACTCACGA CTCATCCACA 
CCCACTCCAT CAGCACTTGA AGAAATGGCC CTGTACAGCA GCAAACGCAA GCTCCGACAC 
AGCCGGCGCA GCCTGGAGGC TGCCGTCCCT ACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCRIB ( XM_014945724.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014801210.1 Calidris pugnax scribbled planar cell polarity protein (SCRIB), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OCa67756
Clone ID Related Accession (Same CDS sequence)	XM_014945724.1
Accession Version	XM_014945724.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5784bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	protein scribble homolog isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090871.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCCGC TCTGGCGCTG CAACCGGCAC GTGGAGGCGC TGGACCGGCG GCACTGCTCG 
CTGCAGGCCG TGCCCGAGGA GATCTACCGC TACAGCCGCT CGCTGGAGGA GCTGCTGCTG 
GACGCCAACC AGCTGCGGGA GCTGCCCAAG CCTTTCTTCA GGCTTCTGAA CCTGCGCAAG 
CTGGGCCTGA GTGACAATGA GATCCAAAGA CTTCCCCCTG AGGTGGCCAA CTTCATGCAG 
CTGGTGGAAC TGGACATCTC CCGTAACGAC ATTCCAGAAA TTCCTGAAAG CATCAAATTC 
TGCAAATCCT TGGAAATCGC AGACTTCAGT GGGAATCCTC TCTCCAGGCT TCCCGAGGGC 
TTCACGCAGC TTCGGAGCCT GGGCCATTTG GCCCTGAATG ATGTGTCCCT GCAGAGCCTC 
CCCAGTGACA TTGGGAATTT GGCAAACCTG GTGACTCTGG AGCTACGCGA GAACCTGCTA 
AAGACTCTCC CCACCTCGCT GTCCTTCCTT GTCAAGTTAG AGCAGCTGGA TCTCGGTGGC 
AATGACCTGG AGGTTCTGCC AGACACTCTG GGAGCGCTCC CCAACCTGCG TGAGCTCTGG 
TTAGACCGGA ACCAGCTCTC AGCCCTGCCT CCCGAGCTGG GCAACCTCCG CCGCCTGGTC 
TGCCTGGATG TGTCGGAGAA CAAGCTGGAG CAGTTGCCCA ACGAGGTCAG CGGCCTGGTG 
GCACTGACCG ACTTGCTGCT GTCGCAGAAC CTGTTGGAGT GCATTCCTGA TGGGATTGGT 
CAGCTGAAGC AGCTCTCCAT CCTCAAGGTG GACCAGAATC GACTGACAGA GGTGACAGAG 
TCCATTGGGG ACTGTGAAAA TCTGTCCGAG CTCATCCTGA CAGAGAACAT GCTGACGGCC 
TTGCCGAAAT CCCTTGGGAA GCTGGCCAAG CTGACCAACC TGAACGTGGA TCGGAACCGG 
CTGACATCCC TCCCGGCCGA GATCGGTGGC TGTGCCAACC TCAACGTGCT GTCCTTGCGG 
GACAATCGCC TGACCCTCCT GCCATCGGAG CTGGCCAACA CCACCGAGCT TCATGTTCTG 
GATGTGGCTG GGAACAGGCT GCAGAACTTG CCGTTTGCTC TGACAAACCT CAACCTGAAA 
GCCCTGTGGC TGGCGGAGAA TCAGTCCCAG CCCATGCTGA AATTCCAAAC AGAGGATGAT 
GAGAAGACAG GAGAGAAAGT TCTCACTTGT TACCTGCTTC CTCAGCAGCC CTCTCCCAGC 
CTGGAGAACC TTCTACAGAA CAGCGTGGAC GAGAGCTGGA CGGACACCAA TTTAAACAGA 
GTCAGTGTGA TTCAGTTCCT GGATGAACCC AAAGCAGATG ATGAAGAAGA GTCTGGAGCT 
GAGAGAAGGG GCTTGCAGCG CAGAGCAACC CCCCATCCCA GCGAGCTGAA GGTGATGAAG 
AAGGTGATTG AAGTTCGGCG CAGTGAGGCC CACGCTGCAA AGCCCAATGC AGACCTGAAT 
TCTCCTCACT CAGAAGAGAA GAGGCTGAGC GCCACGTCCA ACCGCAGCGA GGACTCCCAC 
CTTTCCAACA GCACTGCCTC TGCTGACTGT AGGGCGGAGG AGCAGCAGGA GGAGGCAGAG 
AGGAGTCGGC CAAACGGGCA AGTGCCTGAG ATGCAGGAGC TGGAGAAGGA GGCAAAGTCC 
AGAGTGGACG AGGAGCACAA GGAAGCTGCT GGCCATGAAG AGGAAGATGT GGAAGTGGAA 
TACAATGAGC CCACTGTACA CTTTGCTGAA GACACGCTAA TACGGAGTGA GGATGAAGAT 
GAAGATGAAG AGCGACCGTT CCCAGTGGAG AAGCAGCGGC TCATCCGCAA GGACACCCCT 
CACTATAAGA AACACTTTAA GATAACTAAA TTGCCCAAGC CAGAGGCGGT GGTGGCCCTG 
CTGCAGGGGC TGAACAGCGA GGGGGTCCCC AAGGAGGAAG AGGAGTTGGG AGGCTGCAAC 
AACAACACAG AGCCTGCAGA CCAGGAGGAG GTGTGGGATG AGGAGGACCG GAAGAATGGA 
GCTTTGTCTG CCCAGCCATC GGTGAAGGGG GTGTCGTTTG ATCAAGCCAA TAATCTGCTG 
ATTGAACCTG CTCGCATTGA GGAGGAAGAG TTGACTCTGA CTATCGTACG GCAGACGGGG 
GGGCTGGGCA TCAGCATCGC AGGGGGCAAG GGCTCCACCC CCTATAAGGG CGATGATGAG 
GGCATCTTTA TTTCTCGGGT GTCAGAAGAA GGTCCTGCGG CTCGTGCAGG GGTTCGTGTT 
GGGGACAAGC TCCTGGAGGT GAACGGTGTG TCCCTGCACT CCGCTGAGCA CCACGTGGCA 
GTGGAGGCTC TGCGTGGATC GGGAAGTTCC GTCTCCATGA CTGTTCTGCG GGAGCGGATG 
GTGGAGCCGG AGAATGCCAT CACTGTCACC CCGCTGCGCC CCGAGGATGA CTACAGCCCC 
CGTGAGAGGC GAGGTGGCCT GCGCTTCCCG GAGCGACCCG AGGGGACACC TCCCACCGAG 
AGATACTCCA CGTGCCTCAT GCGCAACGAG AAGGGACTGG GCTTCAGTAT CGCTGGTGGC 
AAAGGCTCCA CTCCTTACCG GGCTGGTGAC ATGGGGATCT TTATCTCCCG GATCGCAGAG 
GGTGGTGCAG CCCATCGGGA CGGGATCCTG CACGTAGGAG ATCGGGTCAT CTCGATCAAT 
GGGGTAGACA TGACAGAAGC CAGGCATGAT CAGGCAGTAG CGCTGCTTAC CGCGTCCTCC 
CCCACCATCG TGCTGCTGGT AGAGAGAGAG GGTGCGGAGC AGCCCGGCGA GGGAGACTGG 
CCCGGGGGGC CACGGGCACG CATGCACTCC CCACCGCCAC CCCCCAGCCA TGGGGAGAGC 
CCGCCCGAGG AGACGCCCGC ACCACAGAGG AACCATCTCT CCAAAGGCCT GGAGGATCAG 
TACCCCATTG AGGAAATTCA CCTGGTGAAA GCAGGTGGTC CCCTGGGGCT CAGCATCGTA 
GGGGGCAGTG ACCACTCAAG CCATCCGTTT GGGATTCATG AACCAGGTGT CTTCATTTCG 
AAGGTCATTC CCCGTGGGCT GGCATCCCGC AGTGGGCTGC GAGTAGGGGA CAGGATCCTG 
GAAGTCAATA GCATTGACCT GCGCCACGCT ACCCACCAGG AAGCGGTGAA CGCCCTGCTC 
TCCAACACCC AGGAGCTGAC CATGTTAGTG AGGCGAGACC CACCACCACC TGGTATGCAG 
GAAATATGCA TTGAGAAGGC TCCAGGAGAA AAGCTGGGCA TCAGCATCCG GGGTGGAGCG 
AAAGGCCATG CTGGAAATCC GTTTGATCCC ACTGACGAGG GCATCTTCAT CTCTAAGGTG 
AGCTCCTCGG GGGCGGCAGC CCGGGACGGC CGCCTGAAGG TGGGGATGCG GATCCTAGAG 
GTGAACCACC AGAGCCTGCT GGGGATGACG CACACGGAAG CGGTGCAGAT GCTGCGCGGG 
GTGGGCGACG CGCTCCTGGT GCTGGTGTGT GACGGCTTCG ACCCCAAGGC TGCAGCTGCC 
ATCGAGATGT CCCCGGGAAT TATTGCAAAC CCTTTCGCTG CTGGTATCGG ACGCAAGAAT 
AGCCTGGAAA GTATCTCTTC CATCGACCGG GATTTAAGCC CTGAGGAACT GGAGATCCTG 
CAGAAGGAGA TGGAAATGGT GAGAGAAGCA ACTCAGTGGG AGAGAGAAGA AATGGAGAAA 
GTGGAGCGGA TGCGTAGGGA GCGGGAGGCA GCCACCCGGC AGCTTGAGGA GGAGGCGGAG 
AACGTCACCA GCGAGCCCTT GAGACTGGAC TATCGGACCC TGGCTGCGCT GCCGAGCAGT 
GGTCTCCAGA GAGTCAACCG CACCCTCGTT CAGTATAATA AGCCCAGCTC CAGAATGGAG 
GGCGACTGTG CTCTGCTGTC TCTGCCGGTG GCACGGGACA GCCGGGATTC ATGCCCTGAC 
ACGGCTGCTG GCCCCACCAT CGGAAGCGTG GCCAGACCTC CCCGGGACTC CGCAACACCC 
GACCACTGCA CCAGCGGGGC AGAAACGCCC CGGCCTGACC AGCAGCCTCC CACAGGTGGC 
CCCTTGTCCC AGGACCAGCC GCCAAAGACG CCAGAGCAAG AGTGCATGGT GGACTCCCAG 
CCCATCCTCT TCAGTGAGAA CCCCTTTGTG GTGGCCAACC GCAGGGGGAA GGCGGCCGGC 
CGGGCCTGCC TGGGGGGGCC TCCCCTGGGC TATGGCAGGG GGGGAGTGCT GAAGACCAAC 
CTTTACTCCA AGGCTCCTGC AGGCGAGTCT GGGATGGGCA ATTCCAGGCG TGAAACCCCC 
TATTCTCCTG GTAACCAGCA GGCTTCCATT AACTTCATTA CATCGCAGGA TGACAGTAAA 
TCGCCAAAGC CACCCTCACC ACCCTCTCCT GATGAGGTCC CCATCAATGT CAAGCAAGCG 
TACAAGACCT TCGCTGCAGT GCCCTTGTCA CACCCCCTGC TTGGGACACA GGAATTGCCT 
GGTCAGAGCG GCACAGAGAA TCCCAGAAGT GCCCCGGAGG TGGCTGCGCA CAGCCCTGGG 
GGACTGCACA GCCCTGAGCA GAAGTCCTTC CGTGAGAGGC AGAAGTATTT CGAGATTGAG 
GTGAAGCAGC AGCAGATGGA CAAGCCCCCC AAGAGAGTCT CCTTGGTGGG AGAGGACGAC 
CTGAAGAAGA TGAAAGAGGA GGAAGCTCGA AAACTGCAGC AGAAACGTAC CCTTCTGCTG 
GAGGAAGAGG CAGAAGAGGA GGAGATGGCG AAGCAGGCGC CTGAGGTGAG CGTGCAGTCC 
AGTGTCATCA TTGAAGGAGT CGAGTACAAG ATTGAGAGAC TCAACGGGAG GAGCAGCCAG 
TCTCCGGCCA CTCGGCCCTC CGAGGTCAAC GAGAACAGCA GCTCGCAGAG GAATTCACTG 
GAAGAGGGGA TCAAGGCTGA GCAGAGAACC AACTCCATGT CTGGGCTGAG TCCCTTGTAC 
CTTGGAGCAG GGGACGCAGT GGCACCCGTG CGGACGGCCA AAGCTGAACG GCGACACCAG 
GAGCGGTTGC GAATGCAGAG TCCTGAGCTG CTGAGTGGCC AAGAGAAGGA GCTTTCTCCT 
GCAGAACGTC GTGCTCTGGA GGCGGAGAAA CGAGCAATGT GGAGGGCAGC ACGATCCTCA 
GCTCTTGAAG ACAGTATTAA ACAGTACGAG CAGGACCTGG CCAGGAGGCT GTACCAGTCC 
CGACAGCGGG CACCTGCTCC CGGGGCCGGG CCAGCTCCGA TGCCTAGTCC CAAGCAGAGT 
CACGCCTCGG CACCCCCTGG GTCAGGAAGC CATTCCAGGA TGAAATCGCT TGAACAGGAT 
GCTCTGAAAG CCCAGATGGT CATTGCCAAG TCCAAGGAGG GGAAGAAGCG CAGCCCGCTG 
GAGCAGCTGG CAGAGTCCCC CTCGCCAGTT CCTACACCGT CACCGACACC ACTGGAAGAT 
TGCAGTCCCA GGAACGTCAC CTCCCCAGGA AGGCTGTCCA TGTCTGAAAA GAAGTTTGAC 
TACCGGGAAT TTGCTGCTAT TCCTTCCTCC AAACCCGTTT ACGAAATTCA GTCGCCTGAT 
GCTGCTGATG ACCTCAGATA CATCGAGGAC AGGAATAACT CAGTTCCCCA GGAGCAGGAT 
GGGCAGCCCG AGGAAGAGGA CACATTAGGA ACTCACGACT CATCCACACC CACTCCATCA 
GCACTTGAAG AAATGGCCCT GTACAGCAGC AAACGCAAGC TCCGACACAG CCGGCGCAGC 
CTGGAGGCTG CCGTCCCTAC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014801210.1
CDS	6..5789
Translation	MPPLWRCNRHVEALDRRHCSLQAVPEEIYRYSRSLEELLLDANQLRELPKPFFRLLNLRKLGLSDNEIQRLPPEVANFMQLVELDISRNDIPEIPESIKFCKSLEIADFSGNPLSRLPEGFTQLRSLGHLALNDVSLQSLPSDIGNLANLVTLELRENLLKTLPTSLSFLVKLEQLDLGGNDLEVLPDTLGALPNLRELWLDRNQLSALPPELGNLRRLVCLDVSENKLEQLPNEVSGLVALTDLLLSQNLLECIPDGIGQLKQLSILKVDQNRLTEVTESIGDCENLSELILTENMLTALPKSLGKLAKLTNLNVDRNRLTSLPAEIGGCANLNVLSLRDNRLTLLPSELANTTELHVLDVAGNRLQNLPFALTNLNLKALWLAENQSQPMLKFQTEDDEKTGEKVLTCYLLPQQPSPSLENLLQNSVDESWTDTNLNRVSVIQFLDEPKADDEEESGAERRGLQRRATPHPSELKVMKKVIEVRRSEAHAAKPNADLNSPHSEEKRLSATSNRSEDSHLSNSTASADCRAEEQQEEAERSRPNGQVPEMQELEKEAKSRVDEEHKEAAGHEEEDVEVEYNEPTVHFAEDTLIRSEDEDEDEERPFPVEKQRLIRKDTPHYKKHFKITKLPKPEAVVALLQGLNSEGVPKEEEELGGCNNNTEPADQEEVWDEEDRKNGALSAQPSVKGVSFDQANNLLIEPARIEEEELTLTIVRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVRVGDKLLEVNGVSLHSAEHHVAVEALRGSGSSVSMTVLRERMVEPENAITVTPLRPEDDYSPRERRGGLRFPERPEGTPPTERYSTCLMRNEKGLGFSIAGGKGSTPYRAGDMGIFISRIAEGGAAHRDGILHVGDRVISINGVDMTEARHDQAVALLTASSPTIVLLVEREGAEQPGEGDWPGGPRARMHSPPPPPSHGESPPEETPAPQRNHLSKGLEDQYPIEEIHLVKAGGPLGLSIVGGSDHSSHPFGIHEPGVFISKVIPRGLASRSGLRVGDRILEVNSIDLRHATHQEAVNALLSNTQELTMLVRRDPPPPGMQEICIEKAPGEKLGISIRGGAKGHAGNPFDPTDEGIFISKVSSSGAAARDGRLKVGMRILEVNHQSLLGMTHTEAVQMLRGVGDALLVLVCDGFDPKAAAAIEMSPGIIANPFAAGIGRKNSLESISSIDRDLSPEELEILQKEMEMVREATQWEREEMEKVERMRREREAATRQLEEEAENVTSEPLRLDYRTLAALPSSGLQRVNRTLVQYNKPSSRMEGDCALLSLPVARDSRDSCPDTAAGPTIGSVARPPRDSATPDHCTSGAETPRPDQQPPTGGPLSQDQPPKTPEQECMVDSQPILFSENPFVVANRRGKAAGRACLGGPPLGYGRGGVLKTNLYSKAPAGESGMGNSRRETPYSPGNQQASINFITSQDDSKSPKPPSPPSPDEVPINVKQAYKTFAAVPLSHPLLGTQELPGQSGTENPRSAPEVAAHSPGGLHSPEQKSFRERQKYFEIEVKQQQMDKPPKRVSLVGEDDLKKMKEEEARKLQQKRTLLLEEEAEEEEMAKQAPEVSVQSSVIIEGVEYKIERLNGRSSQSPATRPSEVNENSSSQRNSLEEGIKAEQRTNSMSGLSPLYLGAGDAVAPVRTAKAERRHQERLRMQSPELLSGQEKELSPAERRALEAEKRAMWRAARSSALEDSIKQYEQDLARRLYQSRQRAPAPGAGPAPMPSPKQSHASAPPGSGSHSRMKSLEQDALKAQMVIAKSKEGKKRSPLEQLAESPSPVPTPSPTPLEDCSPRNVTSPGRLSMSEKKFDYREFAAIPSSKPVYEIQSPDAADDLRYIEDRNNSVPQEQDGQPEEEDTLGTHDSSTPTPSALEEMALYSSKRKLRHSRRSLEAAVPT

Target ORF information:

RefSeq Version	XM_014945724.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax scribbled planar cell polarity protein (SCRIB), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014945724.1

ORF Insert Sequence:

ATGCCGCCGC TCTGGCGCTG CAACCGGCAC GTGGAGGCGC TGGACCGGCG GCACTGCTCG 
CTGCAGGCCG TGCCCGAGGA GATCTACCGC TACAGCCGCT CGCTGGAGGA GCTGCTGCTG 
GACGCCAACC AGCTGCGGGA GCTGCCCAAG CCTTTCTTCA GGCTTCTGAA CCTGCGCAAG 
CTGGGCCTGA GTGACAATGA GATCCAAAGA CTTCCCCCTG AGGTGGCCAA CTTCATGCAG 
CTGGTGGAAC TGGACATCTC CCGTAACGAC ATTCCAGAAA TTCCTGAAAG CATCAAATTC 
TGCAAATCCT TGGAAATCGC AGACTTCAGT GGGAATCCTC TCTCCAGGCT TCCCGAGGGC 
TTCACGCAGC TTCGGAGCCT GGGCCATTTG GCCCTGAATG ATGTGTCCCT GCAGAGCCTC 
CCCAGTGACA TTGGGAATTT GGCAAACCTG GTGACTCTGG AGCTACGCGA GAACCTGCTA 
AAGACTCTCC CCACCTCGCT GTCCTTCCTT GTCAAGTTAG AGCAGCTGGA TCTCGGTGGC 
AATGACCTGG AGGTTCTGCC AGACACTCTG GGAGCGCTCC CCAACCTGCG TGAGCTCTGG 
TTAGACCGGA ACCAGCTCTC AGCCCTGCCT CCCGAGCTGG GCAACCTCCG CCGCCTGGTC 
TGCCTGGATG TGTCGGAGAA CAAGCTGGAG CAGTTGCCCA ACGAGGTCAG CGGCCTGGTG 
GCACTGACCG ACTTGCTGCT GTCGCAGAAC CTGTTGGAGT GCATTCCTGA TGGGATTGGT 
CAGCTGAAGC AGCTCTCCAT CCTCAAGGTG GACCAGAATC GACTGACAGA GGTGACAGAG 
TCCATTGGGG ACTGTGAAAA TCTGTCCGAG CTCATCCTGA CAGAGAACAT GCTGACGGCC 
TTGCCGAAAT CCCTTGGGAA GCTGGCCAAG CTGACCAACC TGAACGTGGA TCGGAACCGG 
CTGACATCCC TCCCGGCCGA GATCGGTGGC TGTGCCAACC TCAACGTGCT GTCCTTGCGG 
GACAATCGCC TGACCCTCCT GCCATCGGAG CTGGCCAACA CCACCGAGCT TCATGTTCTG 
GATGTGGCTG GGAACAGGCT GCAGAACTTG CCGTTTGCTC TGACAAACCT CAACCTGAAA 
GCCCTGTGGC TGGCGGAGAA TCAGTCCCAG CCCATGCTGA AATTCCAAAC AGAGGATGAT 
GAGAAGACAG GAGAGAAAGT TCTCACTTGT TACCTGCTTC CTCAGCAGCC CTCTCCCAGC 
CTGGAGAACC TTCTACAGAA CAGCGTGGAC GAGAGCTGGA CGGACACCAA TTTAAACAGA 
GTCAGTGTGA TTCAGTTCCT GGATGAACCC AAAGCAGATG ATGAAGAAGA GTCTGGAGCT 
GAGAGAAGGG GCTTGCAGCG CAGAGCAACC CCCCATCCCA GCGAGCTGAA GGTGATGAAG 
AAGGTGATTG AAGTTCGGCG CAGTGAGGCC CACGCTGCAA AGCCCAATGC AGACCTGAAT 
TCTCCTCACT CAGAAGAGAA GAGGCTGAGC GCCACGTCCA ACCGCAGCGA GGACTCCCAC 
CTTTCCAACA GCACTGCCTC TGCTGACTGT AGGGCGGAGG AGCAGCAGGA GGAGGCAGAG 
AGGAGTCGGC CAAACGGGCA AGTGCCTGAG ATGCAGGAGC TGGAGAAGGA GGCAAAGTCC 
AGAGTGGACG AGGAGCACAA GGAAGCTGCT GGCCATGAAG AGGAAGATGT GGAAGTGGAA 
TACAATGAGC CCACTGTACA CTTTGCTGAA GACACGCTAA TACGGAGTGA GGATGAAGAT 
GAAGATGAAG AGCGACCGTT CCCAGTGGAG AAGCAGCGGC TCATCCGCAA GGACACCCCT 
CACTATAAGA AACACTTTAA GATAACTAAA TTGCCCAAGC CAGAGGCGGT GGTGGCCCTG 
CTGCAGGGGC TGAACAGCGA GGGGGTCCCC AAGGAGGAAG AGGAGTTGGG AGGCTGCAAC 
AACAACACAG AGCCTGCAGA CCAGGAGGAG GTGTGGGATG AGGAGGACCG GAAGAATGGA 
GCTTTGTCTG CCCAGCCATC GGTGAAGGGG GTGTCGTTTG ATCAAGCCAA TAATCTGCTG 
ATTGAACCTG CTCGCATTGA GGAGGAAGAG TTGACTCTGA CTATCGTACG GCAGACGGGG 
GGGCTGGGCA TCAGCATCGC AGGGGGCAAG GGCTCCACCC CCTATAAGGG CGATGATGAG 
GGCATCTTTA TTTCTCGGGT GTCAGAAGAA GGTCCTGCGG CTCGTGCAGG GGTTCGTGTT 
GGGGACAAGC TCCTGGAGGT GAACGGTGTG TCCCTGCACT CCGCTGAGCA CCACGTGGCA 
GTGGAGGCTC TGCGTGGATC GGGAAGTTCC GTCTCCATGA CTGTTCTGCG GGAGCGGATG 
GTGGAGCCGG AGAATGCCAT CACTGTCACC CCGCTGCGCC CCGAGGATGA CTACAGCCCC 
CGTGAGAGGC GAGGTGGCCT GCGCTTCCCG GAGCGACCCG AGGGGACACC TCCCACCGAG 
AGATACTCCA CGTGCCTCAT GCGCAACGAG AAGGGACTGG GCTTCAGTAT CGCTGGTGGC 
AAAGGCTCCA CTCCTTACCG GGCTGGTGAC ATGGGGATCT TTATCTCCCG GATCGCAGAG 
GGTGGTGCAG CCCATCGGGA CGGGATCCTG CACGTAGGAG ATCGGGTCAT CTCGATCAAT 
GGGGTAGACA TGACAGAAGC CAGGCATGAT CAGGCAGTAG CGCTGCTTAC CGCGTCCTCC 
CCCACCATCG TGCTGCTGGT AGAGAGAGAG GGTGCGGAGC AGCCCGGCGA GGGAGACTGG 
CCCGGGGGGC CACGGGCACG CATGCACTCC CCACCGCCAC CCCCCAGCCA TGGGGAGAGC 
CCGCCCGAGG AGACGCCCGC ACCACAGAGG AACCATCTCT CCAAAGGCCT GGAGGATCAG 
TACCCCATTG AGGAAATTCA CCTGGTGAAA GCAGGTGGTC CCCTGGGGCT CAGCATCGTA 
GGGGGCAGTG ACCACTCAAG CCATCCGTTT GGGATTCATG AACCAGGTGT CTTCATTTCG 
AAGGTCATTC CCCGTGGGCT GGCATCCCGC AGTGGGCTGC GAGTAGGGGA CAGGATCCTG 
GAAGTCAATA GCATTGACCT GCGCCACGCT ACCCACCAGG AAGCGGTGAA CGCCCTGCTC 
TCCAACACCC AGGAGCTGAC CATGTTAGTG AGGCGAGACC CACCACCACC TGGTATGCAG 
GAAATATGCA TTGAGAAGGC TCCAGGAGAA AAGCTGGGCA TCAGCATCCG GGGTGGAGCG 
AAAGGCCATG CTGGAAATCC GTTTGATCCC ACTGACGAGG GCATCTTCAT CTCTAAGGTG 
AGCTCCTCGG GGGCGGCAGC CCGGGACGGC CGCCTGAAGG TGGGGATGCG GATCCTAGAG 
GTGAACCACC AGAGCCTGCT GGGGATGACG CACACGGAAG CGGTGCAGAT GCTGCGCGGG 
GTGGGCGACG CGCTCCTGGT GCTGGTGTGT GACGGCTTCG ACCCCAAGGC TGCAGCTGCC 
ATCGAGATGT CCCCGGGAAT TATTGCAAAC CCTTTCGCTG CTGGTATCGG ACGCAAGAAT 
AGCCTGGAAA GTATCTCTTC CATCGACCGG GATTTAAGCC CTGAGGAACT GGAGATCCTG 
CAGAAGGAGA TGGAAATGGT GAGAGAAGCA ACTCAGTGGG AGAGAGAAGA AATGGAGAAA 
GTGGAGCGGA TGCGTAGGGA GCGGGAGGCA GCCACCCGGC AGCTTGAGGA GGAGGCGGAG 
AACGTCACCA GCGAGCCCTT GAGACTGGAC TATCGGACCC TGGCTGCGCT GCCGAGCAGT 
GGTCTCCAGA GAGTCAACCG CACCCTCGTT CAGTATAATA AGCCCAGCTC CAGAATGGAG 
GGCGACTGTG CTCTGCTGTC TCTGCCGGTG GCACGGGACA GCCGGGATTC ATGCCCTGAC 
ACGGCTGCTG GCCCCACCAT CGGAAGCGTG GCCAGACCTC CCCGGGACTC CGCAACACCC 
GACCACTGCA CCAGCGGGGC AGAAACGCCC CGGCCTGACC AGCAGCCTCC CACAGGTGGC 
CCCTTGTCCC AGGACCAGCC GCCAAAGACG CCAGAGCAAG AGTGCATGGT GGACTCCCAG 
CCCATCCTCT TCAGTGAGAA CCCCTTTGTG GTGGCCAACC GCAGGGGGAA GGCGGCCGGC 
CGGGCCTGCC TGGGGGGGCC TCCCCTGGGC TATGGCAGGG GGGGAGTGCT GAAGACCAAC 
CTTTACTCCA AGGCTCCTGC AGGCGAGTCT GGGATGGGCA ATTCCAGGCG TGAAACCCCC 
TATTCTCCTG GTAACCAGCA GGCTTCCATT AACTTCATTA CATCGCAGGA TGACAGTAAA 
TCGCCAAAGC CACCCTCACC ACCCTCTCCT GATGAGGTCC CCATCAATGT CAAGCAAGCG 
TACAAGACCT TCGCTGCAGT GCCCTTGTCA CACCCCCTGC TTGGGACACA GGAATTGCCT 
GGTCAGAGCG GCACAGAGAA TCCCAGAAGT GCCCCGGAGG TGGCTGCGCA CAGCCCTGGG 
GGACTGCACA GCCCTGAGCA GAAGTCCTTC CGTGAGAGGC AGAAGTATTT CGAGATTGAG 
GTGAAGCAGC AGCAGATGGA CAAGCCCCCC AAGAGAGTCT CCTTGGTGGG AGAGGACGAC 
CTGAAGAAGA TGAAAGAGGA GGAAGCTCGA AAACTGCAGC AGAAACGTAC CCTTCTGCTG 
GAGGAAGAGG CAGAAGAGGA GGAGATGGCG AAGCAGGCGC CTGAGGTGAG CGTGCAGTCC 
AGTGTCATCA TTGAAGGAGT CGAGTACAAG ATTGAGAGAC TCAACGGGAG GAGCAGCCAG 
TCTCCGGCCA CTCGGCCCTC CGAGGTCAAC GAGAACAGCA GCTCGCAGAG GAATTCACTG 
GAAGAGGGGA TCAAGGCTGA GCAGAGAACC AACTCCATGT CTGGGCTGAG TCCCTTGTAC 
CTTGGAGCAG GGGACGCAGT GGCACCCGTG CGGACGGCCA AAGCTGAACG GCGACACCAG 
GAGCGGTTGC GAATGCAGAG TCCTGAGCTG CTGAGTGGCC AAGAGAAGGA GCTTTCTCCT 
GCAGAACGTC GTGCTCTGGA GGCGGAGAAA CGAGCAATGT GGAGGGCAGC ACGATCCTCA 
GCTCTTGAAG ACAGTATTAA ACAGTACGAG CAGGACCTGG CCAGGAGGCT GTACCAGTCC 
CGACAGCGGG CACCTGCTCC CGGGGCCGGG CCAGCTCCGA TGCCTAGTCC CAAGCAGAGT 
CACGCCTCGG CACCCCCTGG GTCAGGAAGC CATTCCAGGA TGAAATCGCT TGAACAGGAT 
GCTCTGAAAG CCCAGATGGT CATTGCCAAG TCCAAGGAGG GGAAGAAGCG CAGCCCGCTG 
GAGCAGCTGG CAGAGTCCCC CTCGCCAGTT CCTACACCGT CACCGACACC ACTGGAAGAT 
TGCAGTCCCA GGAACGTCAC CTCCCCAGGA AGGCTGTCCA TGTCTGAAAA GAAGTTTGAC 
TACCGGGAAT TTGCTGCTAT TCCTTCCTCC AAACCCGTTT ACGAAATTCA GTCGCCTGAT 
GCTGCTGATG ACCTCAGATA CATCGAGGAC AGGAATAACT CAGTTCCCCA GGAGCAGGAT 
GGGCAGCCCG AGGAAGAGGA CACATTAGGA ACTCACGACT CATCCACACC CACTCCATCA 
GCACTTGAAG AAATGGCCCT GTACAGCAGC AAACGCAAGC TCCGACACAG CCGGCGCAGC 
CTGGAGGCTG CCGTCCCTAC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCRIB ( XM_014945725.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014801211.1 Calidris pugnax scribbled planar cell polarity protein (SCRIB), transcript variant X11, mRNA.

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CloneID	OCa67757
Clone ID Related Accession (Same CDS sequence)	XM_014945725.1
Accession Version	XM_014945725.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	protein scribble homolog isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090871.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCCGC TCTGGCGCTG CAACCGGCAC GTGGAGGCGC TGGACCGGCG GCACTGCTCG 
CTGCAGGCCG TGCCCGAGGA GATCTACCGC TACAGCCGCT CGCTGGAGGA GCTGCTGCTG 
GACGCCAACC AGCTGCGGGA GCTGCCCAAG CCTTTCTTCA GGCTTCTGAA CCTGCGCAAG 
CTGGGCCTGA GTGACAATGA GATCCAAAGA CTTCCCCCTG AGGTGGCCAA CTTCATGCAG 
CTGGTGGAAC TGGACATCTC CCGTAACGAC ATTCCAGAAA TTCCTGAAAG CATCAAATTC 
TGCAAATCCT TGGAAATCGC AGACTTCAGT GGGAATCCTC TCTCCAGGCT TCCCGAGGGC 
TTCACGCAGC TTCGGAGCCT GGGCCATTTG GCCCTGAATG ATGTGTCCCT GCAGAGCCTC 
CCCAGTGACA TTGGGAATTT GGCAAACCTG GTGACTCTGG AGCTACGCGA GAACCTGCTA 
AAGACTCTCC CCACCTCGCT GTCCTTCCTT GTCAAGTTAG AGCAGCTGGA TCTCGGTGGC 
AATGACCTGG AGGTTCTGCC AGACACTCTG GGAGCGCTCC CCAACCTGCG TGAGCTCTGG 
TTAGACCGGA ACCAGCTCTC AGCCCTGCCT CCCGAGCTGG GCAACCTCCG CCGCCTGGTC 
TGCCTGGATG TGTCGGAGAA CAAGCTGGAG CAGTTGCCCA ACGAGGTCAG CGGCCTGGTG 
GCACTGACCG ACTTGCTGCT GTCGCAGAAC CTGTTGGAGT GCATTCCTGA TGGGATTGGT 
CAGCTGAAGC AGCTCTCCAT CCTCAAGGTG GACCAGAATC GACTGACAGA GGTGACAGAG 
TCCATTGGGG ACTGTGAAAA TCTGTCCGAG CTCATCCTGA CAGAGAACAT GCTGACGGCC 
TTGCCGAAAT CCCTTGGGAA GCTGGCCAAG CTGACCAACC TGAACGTGGA TCGGAACCGG 
CTGACATCCC TCCCGGCCGA GATCGGTGGC TGTGCCAACC TCAACGTGCT GTCCTTGCGG 
GACAATCGCC TGACCCTCCT GCCATCGGAG CTGGCCAACA CCACCGAGCT TCATGTTCTG 
GATGTGGCTG GGAACAGGCT GCAGAACTTG CCGTTTGCTC TGACAAACCT CAACCTGAAA 
GCCCTGTGGC TGGCGGAGAA TCAGTCCCAG CCCATGCTGA AATTCCAAAC AGAGGATGAT 
GAGAAGACAG GAGAGAAAGT TCTCACTTGT TACCTGCTTC CTCAGCAGCC CTCTCCCAGC 
CTGGAGAACC TTCTACAGAA CAGCGTGGAC GAGAGCTGGA CGGACACCAA TTTAAACAGA 
GTCAGTGTGA TTCAGTTCCT GGATGAACCC AAAGCAGATG ATGAAGAAGA GTCTGGAGCT 
GAGAGAAGGG GCTTGCAGCG CAGAGCAACC CCCCATCCCA GCGAGCTGAA GGTGATGAAG 
AAGGTGATTG AAGTTCGGCG CAGTGAGGCC CACGCTGCAA AGCCCAATGC AGACCTGAAT 
TCTCCTCACT CAGAAGAGAA GAGGCTGAGC GCCACGTCCA ACCGCAGCGA GGACTCCCAC 
CTTTCCAACA GCACTGCCTC TGCTGACTGT AGGGCGGAGG AGCAGCAGGA GGAGGCAGAG 
AGGAGTCGGC CAAACGGGCA AGTGCCTGAG ATGCAGGAGC TGGAGAAGGA GGCAAAGTCC 
AGAGTGGACG AGGAGCACAA GGAAGCTGCT GGCCATGAAG AGGAAGATGT GGAAGTGGAA 
TACAATGAGC CCACTGTACA CTTTGCTGAA GACACGCTAA TACGGAGTGA GGATGAAGAT 
GAAGATGAAG AGCGACCGTT CCCAGTGGAG AAGCAGCGGC TCATCCGCAA GGACACCCCT 
CACTATAAGA AACACTTTAA GATAACTAAA TTGCCCAAGC CAGAGGCGGT GGTGGCCCTG 
CTGCAGGGGC TGAACAGCGA GGGGGTCCCC AAGGAGGAAG AGGAGTTGGG AGGCTGCAAC 
AACAACACAG AGCCTGCAGA CCAGGAGGAG GTGTGGGATG AGGAGGACCG GAAGAATGGA 
GCTTTGTCTG CCCAGCCATC GGTGAAGGGG GTGTCGTTTG ATCAAGCCAA TAATCTGCTG 
ATTGAACCTG CTCGCATTGA GGAGGAAGAG TTGACTCTGA CTATCGTACG GCAGACGGGG 
GGGCTGGGCA TCAGCATCGC AGGGGGCAAG GGCTCCACCC CCTATAAGGG CGATGATGAG 
GGCATCTTTA TTTCTCGGGT GTCAGAAGAA GGTCCTGCGG CTCGTGCAGG GGTTCGTGTT 
GGGGACAAGC TCCTGGAGGT GAACGGTGTG TCCCTGCACT CCGCTGAGCA CCACGTGGCA 
GTGGAGGCTC TGCGTGGATC GGGAAGTTCC GTCTCCATGA CTGTTCTGCG GGAGCGGATG 
GTGGAGCCGG AGAATGCCAT CACTGTCACC CCGCTGCGCC CCGAGGATGA CTACAGCCCC 
CGTGAGAGGC GAGGTGGCCT GCGCTTCCCG GAGCGACCCG AGGGGACACC TCCCACCGAG 
AGATACTCCA CGTGCCTCAT GCGCAACGAG AAGGGACTGG GCTTCAGTAT CGCTGGTGGC 
AAAGGCTCCA CTCCTTACCG GGCTGGTGAC ATGGGGATCT TTATCTCCCG GATCGCAGAG 
GGTGGTGCAG CCCATCGGGA CGGGATCCTG CACGTAGGAG ATCGGGTCAT CTCGATCAAT 
GGGGTAGACA TGACAGAAGC CAGGCATGAT CAGGCAGTAG CGCTGCTTAC CGCGTCCTCC 
CCCACCATCG TGCTGCTGGT AGAGAGAGAG GGTGCGGAGC AGCCCGGCGA GGGAGACTGG 
CCCGGGGGGC CACGGGCACG CATGCACTCC CCACCGCCAC CCCCCAGCCA TGGGGAGAGC 
CCGCCCGAGG AGACGCCCGC ACCACAGAGG AACCATCTCT CCAAAGGCCT GGAGGATCAG 
TACCCCATTG AGGAAATTCA CCTGGTGAAA GCAGGTGGTC CCCTGGGGCT CAGCATCGTA 
GGGGGCAGTG ACCACTCAAG CCATCCGTTT GGGATTCATG AACCAGGTGT CTTCATTTCG 
AAGGTCATTC CCCGTGGGCT GGCATCCCGC AGTGGGCTGC GAGTAGGGGA CAGGATCCTG 
GAAGTCAATA GCATTGACCT GCGCCACGCT ACCCACCAGG AAGCGGTGAA CGCCCTGCTC 
TCCAACACCC AGGAGCTGAC CATGTTAGTG AGGCGAGACC CACCACCACC TGGTATGCAG 
GAAATATGCA TTGAGAAGGC TCCAGGAGAA AAGCTGGGCA TCAGCATCCG GGGTGGAGCG 
AAAGGCCATG CTGGAAATCC GTTTGATCCC ACTGACGAGG GCATCTTCAT CTCTAAGGTG 
AGCTCCTCGG GGGCGGCAGC CCGGGACGGC CGCCTGAAGG TGGGGATGCG GATCCTAGAG 
GTGAACCACC AGAGCCTGCT GGGGATGACG CACACGGAAG CGGTGCAGAT GCTGCGCGGG 
GTGGGCGACG CGCTCCTGGT GCTGGTGTGT GACGGCTTCG ACCCCAAGGC TGCAGCTGCC 
ATCGAGATGT CCCCGGGAAT TATTGCAAAC CCTTTCGCTG CTGGTATCGG ACGCAAGAAT 
AGCCTGGAAA GTATCTCTTC CATCGACCGG GATTTAAGCC CTGAGGAACT GGAGATCCTG 
CAGAAGGAGA TGGAAATGGT GAGAGAAGCA ACTCAGTGGG AGAGAGAAGA AATGGAGAAA 
GTGGAGCGGA TGCGTAGGGA GCGGGAGGCA GCCACCCGGC AGCTTGAGGA GGAGGCGGAG 
AACGTCACCA GCGAGCCCTT GAGACTGGAC TATCGGACCC TGGCTGCGCT GCCGAGCAGT 
GGTCTCCAGA GAGTCAACCG CACCCTCGTT CAGTATAATA AGCCCAGCTC CAGAATGGAG 
GGCGACTGTG CTCTGCTGTC TCTGCCGGTG GCACGGGACA GCCGGGATTC ATGCCCTGAC 
ACGGCTGCTG GCCCCACCAT CGGAAGCGTG GCCAGACCTC CCCGGGACTC CGCAACACCC 
GACCACTGCA CCAGCGGGGC AGAAACGCCC CGGCCTGACC AGCAGCCTCC CACAGGTGGC 
CCCTTGTCCC AGGACCAGCC GCCAAAGACG CCAGAGCAAG AGTGCATGGT GGACTCCCAG 
CCCATCCTCT TCAGTGAGAA CCCCTTTGTG GTGGCCAACC GCAGGGGGAA GGCGGCCGGC 
CGGGCCTGCC TGGGGGGGCC TCCCCTGGGC TATGGCAGGG GGGGAGTGCT GAAGACCAAC 
CTTTACTCCA AGGCTCCTGC AGGCGAGTCT GGGATGGGCA ATTCCAGGCG TGAAACCCCC 
TATTCTCCTG GTAACCAGCA GCGGCCTGCA ACCCCTCGGC CCGCTCACCT GCCGGGCAGG 
GAGACCCGCT TTGCTTCCAT TAACTTCATT ACATCGCAGG ATGACAGTAA ATCGACCAAG 
CCAGGCGTCA TTCAGCCTCT TTCCCGAGTG CGACAAAATG CTGCCCCGCA CAATTCGGAG 
GAGGGGGACA GCCCCAATCC CTTCCAGCAG CCCGAGCCGT GCTTCAGTGT CCAGAACTCT 
CCAAAGCCAC CCTCACCACC CTCTCCTGAT GAGGTCCCCA TCAATGTCAA GCAAGCGTAC 
AAGACCTTCG CTGCAGTGCC CTTGTCACAC CCCCTGCTTG GGACACAGGA ATTGCCTGGT 
CAGAGCGGCA CAGAGAATCC CAGAAGTGCC CCGGAGGTGG CTGCGCACAG CCCTGGGGGA 
CTGCACAGCC CTGAGCAGAA GTCCTTCCGT GAGAGGCAGA AGTATTTCGA GATTGAGGTG 
AAGCAGCAGC AGATGGACAA GCCCCCCAAG AGAGTCTCCT TGGTGGGAGA GGACGACCTG 
AAGAAGATGA AAGAGGAGGA AGCTCGAAAA CTGCAGCAGA AACGTACCCT TCTGCTGGAG 
GAAGAGGCAG AAGAGGAGGA GATGGCGAAG CAGGCGCCTG AGGTGAGCGT GCAGTCCAGT 
GTCATCATTG AAGGAGTCGA GTACAAGATT GAGAGACTCA ACGGGAGGAG CAGCCAGTCT 
CCGGCCACTC GGCCCTCCGA GGTCAACGAG AACAGCAGCT CGCAGAGGAA TTCACTGGAA 
GAGGGGATCA AGGCTGAGCA GAGAACCAAC TCCATGTCTG GGCTGAGTCC CTTGTACCTT 
GGAGCAGGGG ACGCAGTGGC ACCCGTGCGG ACGGCCAAAG CTGAACGGCG ACACCAGGAG 
CGGTTGCGAA TGCAGAGTCC TGAGCTGCTG AGTGGCCAAG AGAAGGAGCT TTCTCCTGCA 
GAACGTCGTG CTCTGGAGGC GGAGAAACGA GCAATGTGGA GGGCAGCACG GATGAAATCG 
CTTGAACAGG ATGCTCTGAA AGCCCAGATG GTCATTGCCA AGTCCAAGGA GGGGAAGAAG 
CGCAGCCCGC TGGAGCAGCT GGCAGAGTCC CCCTCGCCAG TTCCTACACC GTCACCGACA 
CCACTGGAAG ATTGCAGTCC CAGGAACGTC ACCTCCCCAG GAAGGCTGTC GCCTGATGCT 
GCTGATGACC TCAGATACAT CGAGGACAGG AATAACTCAG TTCCCCAGGA GCAGGATGGG 
CAGCCCGAGG AAGAGGACAC ATTAGGAACT CACGACTCAT CCACACCCAC TCCATCAGCA 
CTTGAAGAAA TGGCCCTGTA CAGCAGCAAA CGCAAGCTCC GACACAGCCG GCGCAGCCTG 
GAGGCTGCCG TCCCTACGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014801211.1
CDS	6..5726
Translation	MPPLWRCNRHVEALDRRHCSLQAVPEEIYRYSRSLEELLLDANQLRELPKPFFRLLNLRKLGLSDNEIQRLPPEVANFMQLVELDISRNDIPEIPESIKFCKSLEIADFSGNPLSRLPEGFTQLRSLGHLALNDVSLQSLPSDIGNLANLVTLELRENLLKTLPTSLSFLVKLEQLDLGGNDLEVLPDTLGALPNLRELWLDRNQLSALPPELGNLRRLVCLDVSENKLEQLPNEVSGLVALTDLLLSQNLLECIPDGIGQLKQLSILKVDQNRLTEVTESIGDCENLSELILTENMLTALPKSLGKLAKLTNLNVDRNRLTSLPAEIGGCANLNVLSLRDNRLTLLPSELANTTELHVLDVAGNRLQNLPFALTNLNLKALWLAENQSQPMLKFQTEDDEKTGEKVLTCYLLPQQPSPSLENLLQNSVDESWTDTNLNRVSVIQFLDEPKADDEEESGAERRGLQRRATPHPSELKVMKKVIEVRRSEAHAAKPNADLNSPHSEEKRLSATSNRSEDSHLSNSTASADCRAEEQQEEAERSRPNGQVPEMQELEKEAKSRVDEEHKEAAGHEEEDVEVEYNEPTVHFAEDTLIRSEDEDEDEERPFPVEKQRLIRKDTPHYKKHFKITKLPKPEAVVALLQGLNSEGVPKEEEELGGCNNNTEPADQEEVWDEEDRKNGALSAQPSVKGVSFDQANNLLIEPARIEEEELTLTIVRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVRVGDKLLEVNGVSLHSAEHHVAVEALRGSGSSVSMTVLRERMVEPENAITVTPLRPEDDYSPRERRGGLRFPERPEGTPPTERYSTCLMRNEKGLGFSIAGGKGSTPYRAGDMGIFISRIAEGGAAHRDGILHVGDRVISINGVDMTEARHDQAVALLTASSPTIVLLVEREGAEQPGEGDWPGGPRARMHSPPPPPSHGESPPEETPAPQRNHLSKGLEDQYPIEEIHLVKAGGPLGLSIVGGSDHSSHPFGIHEPGVFISKVIPRGLASRSGLRVGDRILEVNSIDLRHATHQEAVNALLSNTQELTMLVRRDPPPPGMQEICIEKAPGEKLGISIRGGAKGHAGNPFDPTDEGIFISKVSSSGAAARDGRLKVGMRILEVNHQSLLGMTHTEAVQMLRGVGDALLVLVCDGFDPKAAAAIEMSPGIIANPFAAGIGRKNSLESISSIDRDLSPEELEILQKEMEMVREATQWEREEMEKVERMRREREAATRQLEEEAENVTSEPLRLDYRTLAALPSSGLQRVNRTLVQYNKPSSRMEGDCALLSLPVARDSRDSCPDTAAGPTIGSVARPPRDSATPDHCTSGAETPRPDQQPPTGGPLSQDQPPKTPEQECMVDSQPILFSENPFVVANRRGKAAGRACLGGPPLGYGRGGVLKTNLYSKAPAGESGMGNSRRETPYSPGNQQRPATPRPAHLPGRETRFASINFITSQDDSKSTKPGVIQPLSRVRQNAAPHNSEEGDSPNPFQQPEPCFSVQNSPKPPSPPSPDEVPINVKQAYKTFAAVPLSHPLLGTQELPGQSGTENPRSAPEVAAHSPGGLHSPEQKSFRERQKYFEIEVKQQQMDKPPKRVSLVGEDDLKKMKEEEARKLQQKRTLLLEEEAEEEEMAKQAPEVSVQSSVIIEGVEYKIERLNGRSSQSPATRPSEVNENSSSQRNSLEEGIKAEQRTNSMSGLSPLYLGAGDAVAPVRTAKAERRHQERLRMQSPELLSGQEKELSPAERRALEAEKRAMWRAARMKSLEQDALKAQMVIAKSKEGKKRSPLEQLAESPSPVPTPSPTPLEDCSPRNVTSPGRLSPDAADDLRYIEDRNNSVPQEQDGQPEEEDTLGTHDSSTPTPSALEEMALYSSKRKLRHSRRSLEAAVPT

Target ORF information:

RefSeq Version	XM_014945725.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax scribbled planar cell polarity protein (SCRIB), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014945725.1

ORF Insert Sequence:

ATGCCGCCGC TCTGGCGCTG CAACCGGCAC GTGGAGGCGC TGGACCGGCG GCACTGCTCG 
CTGCAGGCCG TGCCCGAGGA GATCTACCGC TACAGCCGCT CGCTGGAGGA GCTGCTGCTG 
GACGCCAACC AGCTGCGGGA GCTGCCCAAG CCTTTCTTCA GGCTTCTGAA CCTGCGCAAG 
CTGGGCCTGA GTGACAATGA GATCCAAAGA CTTCCCCCTG AGGTGGCCAA CTTCATGCAG 
CTGGTGGAAC TGGACATCTC CCGTAACGAC ATTCCAGAAA TTCCTGAAAG CATCAAATTC 
TGCAAATCCT TGGAAATCGC AGACTTCAGT GGGAATCCTC TCTCCAGGCT TCCCGAGGGC 
TTCACGCAGC TTCGGAGCCT GGGCCATTTG GCCCTGAATG ATGTGTCCCT GCAGAGCCTC 
CCCAGTGACA TTGGGAATTT GGCAAACCTG GTGACTCTGG AGCTACGCGA GAACCTGCTA 
AAGACTCTCC CCACCTCGCT GTCCTTCCTT GTCAAGTTAG AGCAGCTGGA TCTCGGTGGC 
AATGACCTGG AGGTTCTGCC AGACACTCTG GGAGCGCTCC CCAACCTGCG TGAGCTCTGG 
TTAGACCGGA ACCAGCTCTC AGCCCTGCCT CCCGAGCTGG GCAACCTCCG CCGCCTGGTC 
TGCCTGGATG TGTCGGAGAA CAAGCTGGAG CAGTTGCCCA ACGAGGTCAG CGGCCTGGTG 
GCACTGACCG ACTTGCTGCT GTCGCAGAAC CTGTTGGAGT GCATTCCTGA TGGGATTGGT 
CAGCTGAAGC AGCTCTCCAT CCTCAAGGTG GACCAGAATC GACTGACAGA GGTGACAGAG 
TCCATTGGGG ACTGTGAAAA TCTGTCCGAG CTCATCCTGA CAGAGAACAT GCTGACGGCC 
TTGCCGAAAT CCCTTGGGAA GCTGGCCAAG CTGACCAACC TGAACGTGGA TCGGAACCGG 
CTGACATCCC TCCCGGCCGA GATCGGTGGC TGTGCCAACC TCAACGTGCT GTCCTTGCGG 
GACAATCGCC TGACCCTCCT GCCATCGGAG CTGGCCAACA CCACCGAGCT TCATGTTCTG 
GATGTGGCTG GGAACAGGCT GCAGAACTTG CCGTTTGCTC TGACAAACCT CAACCTGAAA 
GCCCTGTGGC TGGCGGAGAA TCAGTCCCAG CCCATGCTGA AATTCCAAAC AGAGGATGAT 
GAGAAGACAG GAGAGAAAGT TCTCACTTGT TACCTGCTTC CTCAGCAGCC CTCTCCCAGC 
CTGGAGAACC TTCTACAGAA CAGCGTGGAC GAGAGCTGGA CGGACACCAA TTTAAACAGA 
GTCAGTGTGA TTCAGTTCCT GGATGAACCC AAAGCAGATG ATGAAGAAGA GTCTGGAGCT 
GAGAGAAGGG GCTTGCAGCG CAGAGCAACC CCCCATCCCA GCGAGCTGAA GGTGATGAAG 
AAGGTGATTG AAGTTCGGCG CAGTGAGGCC CACGCTGCAA AGCCCAATGC AGACCTGAAT 
TCTCCTCACT CAGAAGAGAA GAGGCTGAGC GCCACGTCCA ACCGCAGCGA GGACTCCCAC 
CTTTCCAACA GCACTGCCTC TGCTGACTGT AGGGCGGAGG AGCAGCAGGA GGAGGCAGAG 
AGGAGTCGGC CAAACGGGCA AGTGCCTGAG ATGCAGGAGC TGGAGAAGGA GGCAAAGTCC 
AGAGTGGACG AGGAGCACAA GGAAGCTGCT GGCCATGAAG AGGAAGATGT GGAAGTGGAA 
TACAATGAGC CCACTGTACA CTTTGCTGAA GACACGCTAA TACGGAGTGA GGATGAAGAT 
GAAGATGAAG AGCGACCGTT CCCAGTGGAG AAGCAGCGGC TCATCCGCAA GGACACCCCT 
CACTATAAGA AACACTTTAA GATAACTAAA TTGCCCAAGC CAGAGGCGGT GGTGGCCCTG 
CTGCAGGGGC TGAACAGCGA GGGGGTCCCC AAGGAGGAAG AGGAGTTGGG AGGCTGCAAC 
AACAACACAG AGCCTGCAGA CCAGGAGGAG GTGTGGGATG AGGAGGACCG GAAGAATGGA 
GCTTTGTCTG CCCAGCCATC GGTGAAGGGG GTGTCGTTTG ATCAAGCCAA TAATCTGCTG 
ATTGAACCTG CTCGCATTGA GGAGGAAGAG TTGACTCTGA CTATCGTACG GCAGACGGGG 
GGGCTGGGCA TCAGCATCGC AGGGGGCAAG GGCTCCACCC CCTATAAGGG CGATGATGAG 
GGCATCTTTA TTTCTCGGGT GTCAGAAGAA GGTCCTGCGG CTCGTGCAGG GGTTCGTGTT 
GGGGACAAGC TCCTGGAGGT GAACGGTGTG TCCCTGCACT CCGCTGAGCA CCACGTGGCA 
GTGGAGGCTC TGCGTGGATC GGGAAGTTCC GTCTCCATGA CTGTTCTGCG GGAGCGGATG 
GTGGAGCCGG AGAATGCCAT CACTGTCACC CCGCTGCGCC CCGAGGATGA CTACAGCCCC 
CGTGAGAGGC GAGGTGGCCT GCGCTTCCCG GAGCGACCCG AGGGGACACC TCCCACCGAG 
AGATACTCCA CGTGCCTCAT GCGCAACGAG AAGGGACTGG GCTTCAGTAT CGCTGGTGGC 
AAAGGCTCCA CTCCTTACCG GGCTGGTGAC ATGGGGATCT TTATCTCCCG GATCGCAGAG 
GGTGGTGCAG CCCATCGGGA CGGGATCCTG CACGTAGGAG ATCGGGTCAT CTCGATCAAT 
GGGGTAGACA TGACAGAAGC CAGGCATGAT CAGGCAGTAG CGCTGCTTAC CGCGTCCTCC 
CCCACCATCG TGCTGCTGGT AGAGAGAGAG GGTGCGGAGC AGCCCGGCGA GGGAGACTGG 
CCCGGGGGGC CACGGGCACG CATGCACTCC CCACCGCCAC CCCCCAGCCA TGGGGAGAGC 
CCGCCCGAGG AGACGCCCGC ACCACAGAGG AACCATCTCT CCAAAGGCCT GGAGGATCAG 
TACCCCATTG AGGAAATTCA CCTGGTGAAA GCAGGTGGTC CCCTGGGGCT CAGCATCGTA 
GGGGGCAGTG ACCACTCAAG CCATCCGTTT GGGATTCATG AACCAGGTGT CTTCATTTCG 
AAGGTCATTC CCCGTGGGCT GGCATCCCGC AGTGGGCTGC GAGTAGGGGA CAGGATCCTG 
GAAGTCAATA GCATTGACCT GCGCCACGCT ACCCACCAGG AAGCGGTGAA CGCCCTGCTC 
TCCAACACCC AGGAGCTGAC CATGTTAGTG AGGCGAGACC CACCACCACC TGGTATGCAG 
GAAATATGCA TTGAGAAGGC TCCAGGAGAA AAGCTGGGCA TCAGCATCCG GGGTGGAGCG 
AAAGGCCATG CTGGAAATCC GTTTGATCCC ACTGACGAGG GCATCTTCAT CTCTAAGGTG 
AGCTCCTCGG GGGCGGCAGC CCGGGACGGC CGCCTGAAGG TGGGGATGCG GATCCTAGAG 
GTGAACCACC AGAGCCTGCT GGGGATGACG CACACGGAAG CGGTGCAGAT GCTGCGCGGG 
GTGGGCGACG CGCTCCTGGT GCTGGTGTGT GACGGCTTCG ACCCCAAGGC TGCAGCTGCC 
ATCGAGATGT CCCCGGGAAT TATTGCAAAC CCTTTCGCTG CTGGTATCGG ACGCAAGAAT 
AGCCTGGAAA GTATCTCTTC CATCGACCGG GATTTAAGCC CTGAGGAACT GGAGATCCTG 
CAGAAGGAGA TGGAAATGGT GAGAGAAGCA ACTCAGTGGG AGAGAGAAGA AATGGAGAAA 
GTGGAGCGGA TGCGTAGGGA GCGGGAGGCA GCCACCCGGC AGCTTGAGGA GGAGGCGGAG 
AACGTCACCA GCGAGCCCTT GAGACTGGAC TATCGGACCC TGGCTGCGCT GCCGAGCAGT 
GGTCTCCAGA GAGTCAACCG CACCCTCGTT CAGTATAATA AGCCCAGCTC CAGAATGGAG 
GGCGACTGTG CTCTGCTGTC TCTGCCGGTG GCACGGGACA GCCGGGATTC ATGCCCTGAC 
ACGGCTGCTG GCCCCACCAT CGGAAGCGTG GCCAGACCTC CCCGGGACTC CGCAACACCC 
GACCACTGCA CCAGCGGGGC AGAAACGCCC CGGCCTGACC AGCAGCCTCC CACAGGTGGC 
CCCTTGTCCC AGGACCAGCC GCCAAAGACG CCAGAGCAAG AGTGCATGGT GGACTCCCAG 
CCCATCCTCT TCAGTGAGAA CCCCTTTGTG GTGGCCAACC GCAGGGGGAA GGCGGCCGGC 
CGGGCCTGCC TGGGGGGGCC TCCCCTGGGC TATGGCAGGG GGGGAGTGCT GAAGACCAAC 
CTTTACTCCA AGGCTCCTGC AGGCGAGTCT GGGATGGGCA ATTCCAGGCG TGAAACCCCC 
TATTCTCCTG GTAACCAGCA GCGGCCTGCA ACCCCTCGGC CCGCTCACCT GCCGGGCAGG 
GAGACCCGCT TTGCTTCCAT TAACTTCATT ACATCGCAGG ATGACAGTAA ATCGACCAAG 
CCAGGCGTCA TTCAGCCTCT TTCCCGAGTG CGACAAAATG CTGCCCCGCA CAATTCGGAG 
GAGGGGGACA GCCCCAATCC CTTCCAGCAG CCCGAGCCGT GCTTCAGTGT CCAGAACTCT 
CCAAAGCCAC CCTCACCACC CTCTCCTGAT GAGGTCCCCA TCAATGTCAA GCAAGCGTAC 
AAGACCTTCG CTGCAGTGCC CTTGTCACAC CCCCTGCTTG GGACACAGGA ATTGCCTGGT 
CAGAGCGGCA CAGAGAATCC CAGAAGTGCC CCGGAGGTGG CTGCGCACAG CCCTGGGGGA 
CTGCACAGCC CTGAGCAGAA GTCCTTCCGT GAGAGGCAGA AGTATTTCGA GATTGAGGTG 
AAGCAGCAGC AGATGGACAA GCCCCCCAAG AGAGTCTCCT TGGTGGGAGA GGACGACCTG 
AAGAAGATGA AAGAGGAGGA AGCTCGAAAA CTGCAGCAGA AACGTACCCT TCTGCTGGAG 
GAAGAGGCAG AAGAGGAGGA GATGGCGAAG CAGGCGCCTG AGGTGAGCGT GCAGTCCAGT 
GTCATCATTG AAGGAGTCGA GTACAAGATT GAGAGACTCA ACGGGAGGAG CAGCCAGTCT 
CCGGCCACTC GGCCCTCCGA GGTCAACGAG AACAGCAGCT CGCAGAGGAA TTCACTGGAA 
GAGGGGATCA AGGCTGAGCA GAGAACCAAC TCCATGTCTG GGCTGAGTCC CTTGTACCTT 
GGAGCAGGGG ACGCAGTGGC ACCCGTGCGG ACGGCCAAAG CTGAACGGCG ACACCAGGAG 
CGGTTGCGAA TGCAGAGTCC TGAGCTGCTG AGTGGCCAAG AGAAGGAGCT TTCTCCTGCA 
GAACGTCGTG CTCTGGAGGC GGAGAAACGA GCAATGTGGA GGGCAGCACG GATGAAATCG 
CTTGAACAGG ATGCTCTGAA AGCCCAGATG GTCATTGCCA AGTCCAAGGA GGGGAAGAAG 
CGCAGCCCGC TGGAGCAGCT GGCAGAGTCC CCCTCGCCAG TTCCTACACC GTCACCGACA 
CCACTGGAAG ATTGCAGTCC CAGGAACGTC ACCTCCCCAG GAAGGCTGTC GCCTGATGCT 
GCTGATGACC TCAGATACAT CGAGGACAGG AATAACTCAG TTCCCCAGGA GCAGGATGGG 
CAGCCCGAGG AAGAGGACAC ATTAGGAACT CACGACTCAT CCACACCCAC TCCATCAGCA 
CTTGAAGAAA TGGCCCTGTA CAGCAGCAAA CGCAAGCTCC GACACAGCCG GCGCAGCCTG 
GAGGCTGCCG TCCCTACGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCRIB ( XM_014945726.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014801212.1 Calidris pugnax scribbled planar cell polarity protein (SCRIB), transcript variant X12, mRNA.

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CloneID	OCa67758
Clone ID Related Accession (Same CDS sequence)	XM_014945726.1
Accession Version	XM_014945726.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5553bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	protein scribble homolog isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090871.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCCGC TCTGGCGCTG CAACCGGCAC GTGGAGGCGC TGGACCGGCG GCACTGCTCG 
CTGCAGGCCG TGCCCGAGGA GATCTACCGC TACAGCCGCT CGCTGGAGGA GCTGCTGCTG 
GACGCCAACC AGCTGCGGGA GCTGCCCAAG CCTTTCTTCA GGCTTCTGAA CCTGCGCAAG 
CTGGGCCTGA GTGACAATGA GATCCAAAGA CTTCCCCCTG AGGTGGCCAA CTTCATGCAG 
CTGGTGGAAC TGGACATCTC CCGTAACGAC ATTCCAGAAA TTCCTGAAAG CATCAAATTC 
TGCAAATCCT TGGAAATCGC AGACTTCAGT GGGAATCCTC TCTCCAGGCT TCCCGAGGGC 
TTCACGCAGC TTCGGAGCCT GGGCCATTTG GCCCTGAATG ATGTGTCCCT GCAGAGCCTC 
CCCAGTGACA TTGGGAATTT GGCAAACCTG GTGACTCTGG AGCTACGCGA GAACCTGCTA 
AAGACTCTCC CCACCTCGCT GTCCTTCCTT GTCAAGTTAG AGCAGCTGGA TCTCGGTGGC 
AATGACCTGG AGGTTCTGCC AGACACTCTG GGAGCGCTCC CCAACCTGCG TGAGCTCTGG 
TTAGACCGGA ACCAGCTCTC AGCCCTGCCT CCCGAGCTGG GCAACCTCCG CCGCCTGGTC 
TGCCTGGATG TGTCGGAGAA CAAGCTGGAG CAGTTGCCCA ACGAGGTCAG CGGCCTGGTG 
GCACTGACCG ACTTGCTGCT GTCGCAGAAC CTGTTGGAGT GCATTCCTGA TGGGATTGGT 
CAGCTGAAGC AGCTCTCCAT CCTCAAGGTG GACCAGAATC GACTGACAGA GGTGACAGAG 
TCCATTGGGG ACTGTGAAAA TCTGTCCGAG CTCATCCTGA CAGAGAACAT GCTGACGGCC 
TTGCCGAAAT CCCTTGGGAA GCTGGCCAAG CTGACCAACC TGAACGTGGA TCGGAACCGG 
CTGACATCCC TCCCGGCCGA GATCGGTGGC TGTGCCAACC TCAACGTGCT GTCCTTGCGG 
GACAATCGCC TGACCCTCCT GCCATCGGAG CTGGCCAACA CCACCGAGCT TCATGTTCTG 
GATGTGGCTG GGAACAGGCT GCAGAACTTG CCGTTTGCTC TGACAAACCT CAACCTGAAA 
GCCCTGTGGC TGGCGGAGAA TCAGTCCCAG CCCATGCTGA AATTCCAAAC AGAGGATGAT 
GAGAAGACAG GAGAGAAAGT TCTCACTTGT TACCTGCTTC CTCAGCAGCC CTCTCCCAGC 
CTGGAGAACC TTCTACAGAA CAGCGTGGAC GAGAGCTGGA CGGACACCAA TTTAAACAGA 
GTCAGTGTGA TTCAGTTCCT GGATGAACCC AAAGCAGATG ATGAAGAAGA GTCTGGAGCT 
GAGAGAAGGG GCTTGCAGCG CAGAGCAACC CCCCATCCCA GCGAGCTGAA GGTGATGAAG 
AAGGTGATTG AAGTTCGGCG CAGTGAGGCC CACGCTGCAA AGCCCAATGC AGACCTGAAT 
TCTCCTCACT CAGAAGAGAA GAGGCTGAGC GCCACGTCCA ACCGCAGCGA GGACTCCCAC 
CTTTCCAACA GCACTGCCTC TGCTGACTGT AGGGCGGAGG AGCAGCAGGA GGAGGCAGAG 
AGGAGTCGGC CAAACGGGCA AGTGCCTGAG ATGCAGGAGC TGGAGAAGGA GGCAAAGTCC 
AGAGTGGACG AGGAGCACAA GGAAGCTGCT GGCCATGAAG AGGAAGATGT GGAAGTGGAA 
TACAATGAGC CCACTGTACA CTTTGCTGAA GACACGCTAA TACGGAGTGA GGATGAAGAT 
GAAGATGAAG AGCGACCGTT CCCAGTGGAG AAGCAGCGGC TCATCCGCAA GGACACCCCT 
CACTATAAGA AACACTTTAA GATAACTAAA TTGCCCAAGC CAGAGGCGGT GGTGGCCCTG 
CTGCAGGGGC TGAACAGCGA GGGGGTCCCC AAGGAGGAAG AGGAGTTGGG AGGCTGCAAC 
AACAACACAG AGCCTGCAGA CCAGGAGGAG GTGTGGGATG AGGAGGACCG GAAGAATGGA 
GCTTTGTCTG CCCAGCCATC GGTGAAGGGG GTGTCGTTTG ATCAAGCCAA TAATCTGCTG 
ATTGAACCTG CTCGCATTGA GGAGGAAGAG TTGACTCTGA CTATCGTACG GCAGACGGGG 
GGGCTGGGCA TCAGCATCGC AGGGGGCAAG GGCTCCACCC CCTATAAGGG CGATGATGAG 
GGCATCTTTA TTTCTCGGGT GTCAGAAGAA GGTCCTGCGG CTCGTGCAGG GGTTCGTGTT 
GGGGACAAGC TCCTGGAGGT GAACGGTGTG TCCCTGCACT CCGCTGAGCA CCACGTGGCA 
GTGGAGGCTC TGCGTGGATC GGGAAGTTCC GTCTCCATGA CTGTTCTGCG GGAGCGGATG 
GTGGAGCCGG AGAATGCCAT CACTGTCACC CCGCTGCGCC CCGAGGATGA CTACAGCCCC 
CGTGAGAGGC GAGGTGGCCT GCGCTTCCCG GAGCGACCCG AGGGGACACC TCCCACCGAG 
AGATACTCCA CGTGCCTCAT GCGCAACGAG AAGGGACTGG GCTTCAGTAT CGCTGGTGGC 
AAAGGCTCCA CTCCTTACCG GGCTGGTGAC ATGGGGATCT TTATCTCCCG GATCGCAGAG 
GGTGGTGCAG CCCATCGGGA CGGGATCCTG CACGTAGGAG ATCGGGTCAT CTCGATCAAT 
GGGGTAGACA TGACAGAAGC CAGGCATGAT CAGGCAGTAG CGCTGCTTAC CGCGTCCTCC 
CCCACCATCG TGCTGCTGGT AGAGAGAGAG GGTGCGGAGC AGCCCGGCGA GGGAGACTGG 
CCCGGGGGGC CACGGGCACG CATGCACTCC CCACCGCCAC CCCCCAGCCA TGGGGAGAGC 
CCGCCCGAGG AGACGCCCGC ACCACAGAGG AACCATCTCT CCAAAGGCCT GGAGGATCAG 
TACCCCATTG AGGAAATTCA CCTGGTGAAA GCAGGTGGTC CCCTGGGGCT CAGCATCGTA 
GGGGGCAGTG ACCACTCAAG CCATCCGTTT GGGATTCATG AACCAGGTGT CTTCATTTCG 
AAGGTCATTC CCCGTGGGCT GGCATCCCGC AGTGGGCTGC GAGTAGGGGA CAGGATCCTG 
GAAGTCAATA GCATTGACCT GCGCCACGCT ACCCACCAGG AAGCGGTGAA CGCCCTGCTC 
TCCAACACCC AGGAGCTGAC CATGTTAGTG AGGCGAGACC CACCACCACC TGGTATGCAG 
GAAATATGCA TTGAGAAGGC TCCAGGAGAA AAGCTGGGCA TCAGCATCCG GGGTGGAGCG 
AAAGGCCATG CTGGAAATCC GTTTGATCCC ACTGACGAGG GCATCTTCAT CTCTAAGGTG 
AGCTCCTCGG GGGCGGCAGC CCGGGACGGC CGCCTGAAGG TGGGGATGCG GATCCTAGAG 
GTGAACCACC AGAGCCTGCT GGGGATGACG CACACGGAAG CGGTGCAGAT GCTGCGCGGG 
GTGGGCGACG CGCTCCTGGT GCTGGTGTGT GACGGCTTCG ACCCCAAGGC TGCAGCTGCC 
ATCGAGATGT CCCCGGGAAT TATTGCAAAC CCTTTCGCTG CTGGTATCGG ACGCAAGAAT 
AGCCTGGAAA GTATCTCTTC CATCGACCGG GATTTAAGCC CTGAGGAACT GGAGATCCTG 
CAGAAGGAGA TGGAAATGGT GAGAGAAGCA ACTCAGTGGG AGAGAGAAGA AATGGAGAAA 
GTGGAGCGGA TGCGTAGGGA GCGGGAGGCA GCCACCCGGC AGCTTGAGGA GGAGGCGGAG 
AACGTCACCA GCGAGCCCTT GAGACTGGAC TATCGGACCC TGGCTGCGCT GCCGAGCAGT 
GGTCTCCAGA GAGTCAACCG CACCGCTCCT GCAGGCGAGT CTGGGATGGG CAATTCCAGG 
CGTGAAACCC CCTATTCTCC TGGTAACCAG CAGCGGCCTG CAACCCCTCG GCCCGCTCAC 
CTGCCGGGCA GGGAGACCCG CTTTGCTTCC ATTAACTTCA TTACATCGCA GGATGACAGT 
AAATCGACCA AGCCAGGCGT CATTCAGCCT CTTTCCCGAG TGCGACAAAA TGCTGCCCCG 
CACAATTCGG AGGAGGGGGA CAGCCCCAAT CCCTTCCAGC AGCCCGAGCC GTGCTTCAGT 
GTCCAGAACT CTCCAAAGCC ACCCTCACCA CCCTCTCCTG ATGAGGTCCC CATCAATGTC 
AAGCAAGCGT ACAAGACCTT CGCTGCAGTG CCCTTGTCAC ACCCCCTGCT TGGGACACAG 
GAATTGCCTG GTCAGAGCGG CACAGAGAAT CCCAGAAGTG CCCCGGAGGT GGCTGCGCAC 
AGCCCTGGGG GACTGCACAG CCCTGAGCAG AAGTCCTTCC GTGAGAGGCA GAAGTATTTC 
GAGATTGAGG TGAAGCAGCA GCAGATGGAC AAGCCCCCCA AGAGAGTCTC CTTGGTGGGA 
GAGGACGACC TGAAGAAGAT GAAAGAGGAG GAAGCTCGAA AACTGCAGCA GAAACGTACC 
CTTCTGCTGG AGGAAGAGGC AGAAGAGGAG GAGATGGCGA AGCAGGCGCC TGAGGTGAGC 
GTGCAGTCCA GTGTCATCAT TGAAGGAGTC GAGTACAAGA TTGAGAGACT CAACGGGAGG 
AGCAGCCAGT CTCCGGCCAC TCGGCCCTCC GAGGTCAACG AGAACAGCAG CTCGCAGAGG 
AATTCACTGG AAGAGGGGAT CAAGGCTGAG CAGAGAACCA ACTCCATGTC TGGGCTGAGT 
CCCTTGTACC TTGGAGCAGG GGACGCAGTG GCACCCGTGC GGACGGCCAA AGCTGAACGG 
CGACACCAGG AGCGGTTGCG AATGCAGAGT CCTGAGCTGC TGAGTGGCCA AGAGAAGGAG 
CTTTCTCCTG CAGAACGTCG TGCTCTGGAG GCGGAGAAAC GAGCAATGTG GAGGGCAGCA 
CGATCCTCAG CTCTTGAAGA CAGTATTAAA CAGTACGAGC AGGACCTGGC CAGGAGGCTG 
TACCAGTCCC GACAGCGGGC ACCTGCTCCC GGGGCCGGGC CAGCTCCGAT GCCTAGTCCC 
AAGCAGAGTC ACGCCTCGGC ACCCCCTGGG TCAGGAAGCC ATTCCAGGAT GAAATCGCTT 
GAACAGGATG CTCTGAAAGC CCAGATGGTC ATTGCCAAGT CCAAGGAGGG GAAGAAGCGC 
AGCCCGCTGG AGCAGCTGGC AGAGTCCCCC TCGCCAGTTC CTACACCGTC ACCGACACCA 
CTGGAAGATT GCAGTCCCAG GAACGTCACC TCCCCAGGAA GGCTGTCCAT GTCTGAAAAG 
AAGTTTGACT ACCGGGAATT TGCTGCTATT CCTTCCTCCA AACCCGTTTA CGAAATTCAG 
TCGCCTGATG CTGCTGATGA CCTCAGATAC ATCGAGGACA GGAATAACTC AGTTCCCCAG 
GAGCAGGATG GGCAGCCCGA GGAAGAGGAC ACATTAGGAA CTCACGACTC ATCCACACCC 
ACTCCATCAG CACTTGAAGA AATGGCCCTG TACAGCAGCA AACGCAAGCT CCGACACAGC 
CGGCGCAGCC TGGAGGCTGC CGTCCCTACG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014801212.1
CDS	6..5558
Translation	MPPLWRCNRHVEALDRRHCSLQAVPEEIYRYSRSLEELLLDANQLRELPKPFFRLLNLRKLGLSDNEIQRLPPEVANFMQLVELDISRNDIPEIPESIKFCKSLEIADFSGNPLSRLPEGFTQLRSLGHLALNDVSLQSLPSDIGNLANLVTLELRENLLKTLPTSLSFLVKLEQLDLGGNDLEVLPDTLGALPNLRELWLDRNQLSALPPELGNLRRLVCLDVSENKLEQLPNEVSGLVALTDLLLSQNLLECIPDGIGQLKQLSILKVDQNRLTEVTESIGDCENLSELILTENMLTALPKSLGKLAKLTNLNVDRNRLTSLPAEIGGCANLNVLSLRDNRLTLLPSELANTTELHVLDVAGNRLQNLPFALTNLNLKALWLAENQSQPMLKFQTEDDEKTGEKVLTCYLLPQQPSPSLENLLQNSVDESWTDTNLNRVSVIQFLDEPKADDEEESGAERRGLQRRATPHPSELKVMKKVIEVRRSEAHAAKPNADLNSPHSEEKRLSATSNRSEDSHLSNSTASADCRAEEQQEEAERSRPNGQVPEMQELEKEAKSRVDEEHKEAAGHEEEDVEVEYNEPTVHFAEDTLIRSEDEDEDEERPFPVEKQRLIRKDTPHYKKHFKITKLPKPEAVVALLQGLNSEGVPKEEEELGGCNNNTEPADQEEVWDEEDRKNGALSAQPSVKGVSFDQANNLLIEPARIEEEELTLTIVRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVRVGDKLLEVNGVSLHSAEHHVAVEALRGSGSSVSMTVLRERMVEPENAITVTPLRPEDDYSPRERRGGLRFPERPEGTPPTERYSTCLMRNEKGLGFSIAGGKGSTPYRAGDMGIFISRIAEGGAAHRDGILHVGDRVISINGVDMTEARHDQAVALLTASSPTIVLLVEREGAEQPGEGDWPGGPRARMHSPPPPPSHGESPPEETPAPQRNHLSKGLEDQYPIEEIHLVKAGGPLGLSIVGGSDHSSHPFGIHEPGVFISKVIPRGLASRSGLRVGDRILEVNSIDLRHATHQEAVNALLSNTQELTMLVRRDPPPPGMQEICIEKAPGEKLGISIRGGAKGHAGNPFDPTDEGIFISKVSSSGAAARDGRLKVGMRILEVNHQSLLGMTHTEAVQMLRGVGDALLVLVCDGFDPKAAAAIEMSPGIIANPFAAGIGRKNSLESISSIDRDLSPEELEILQKEMEMVREATQWEREEMEKVERMRREREAATRQLEEEAENVTSEPLRLDYRTLAALPSSGLQRVNRTAPAGESGMGNSRRETPYSPGNQQRPATPRPAHLPGRETRFASINFITSQDDSKSTKPGVIQPLSRVRQNAAPHNSEEGDSPNPFQQPEPCFSVQNSPKPPSPPSPDEVPINVKQAYKTFAAVPLSHPLLGTQELPGQSGTENPRSAPEVAAHSPGGLHSPEQKSFRERQKYFEIEVKQQQMDKPPKRVSLVGEDDLKKMKEEEARKLQQKRTLLLEEEAEEEEMAKQAPEVSVQSSVIIEGVEYKIERLNGRSSQSPATRPSEVNENSSSQRNSLEEGIKAEQRTNSMSGLSPLYLGAGDAVAPVRTAKAERRHQERLRMQSPELLSGQEKELSPAERRALEAEKRAMWRAARSSALEDSIKQYEQDLARRLYQSRQRAPAPGAGPAPMPSPKQSHASAPPGSGSHSRMKSLEQDALKAQMVIAKSKEGKKRSPLEQLAESPSPVPTPSPTPLEDCSPRNVTSPGRLSMSEKKFDYREFAAIPSSKPVYEIQSPDAADDLRYIEDRNNSVPQEQDGQPEEEDTLGTHDSSTPTPSALEEMALYSSKRKLRHSRRSLEAAVPT

Target ORF information:

RefSeq Version	XM_014945726.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax scribbled planar cell polarity protein (SCRIB), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014945726.1

ORF Insert Sequence:

ATGCCGCCGC TCTGGCGCTG CAACCGGCAC GTGGAGGCGC TGGACCGGCG GCACTGCTCG 
CTGCAGGCCG TGCCCGAGGA GATCTACCGC TACAGCCGCT CGCTGGAGGA GCTGCTGCTG 
GACGCCAACC AGCTGCGGGA GCTGCCCAAG CCTTTCTTCA GGCTTCTGAA CCTGCGCAAG 
CTGGGCCTGA GTGACAATGA GATCCAAAGA CTTCCCCCTG AGGTGGCCAA CTTCATGCAG 
CTGGTGGAAC TGGACATCTC CCGTAACGAC ATTCCAGAAA TTCCTGAAAG CATCAAATTC 
TGCAAATCCT TGGAAATCGC AGACTTCAGT GGGAATCCTC TCTCCAGGCT TCCCGAGGGC 
TTCACGCAGC TTCGGAGCCT GGGCCATTTG GCCCTGAATG ATGTGTCCCT GCAGAGCCTC 
CCCAGTGACA TTGGGAATTT GGCAAACCTG GTGACTCTGG AGCTACGCGA GAACCTGCTA 
AAGACTCTCC CCACCTCGCT GTCCTTCCTT GTCAAGTTAG AGCAGCTGGA TCTCGGTGGC 
AATGACCTGG AGGTTCTGCC AGACACTCTG GGAGCGCTCC CCAACCTGCG TGAGCTCTGG 
TTAGACCGGA ACCAGCTCTC AGCCCTGCCT CCCGAGCTGG GCAACCTCCG CCGCCTGGTC 
TGCCTGGATG TGTCGGAGAA CAAGCTGGAG CAGTTGCCCA ACGAGGTCAG CGGCCTGGTG 
GCACTGACCG ACTTGCTGCT GTCGCAGAAC CTGTTGGAGT GCATTCCTGA TGGGATTGGT 
CAGCTGAAGC AGCTCTCCAT CCTCAAGGTG GACCAGAATC GACTGACAGA GGTGACAGAG 
TCCATTGGGG ACTGTGAAAA TCTGTCCGAG CTCATCCTGA CAGAGAACAT GCTGACGGCC 
TTGCCGAAAT CCCTTGGGAA GCTGGCCAAG CTGACCAACC TGAACGTGGA TCGGAACCGG 
CTGACATCCC TCCCGGCCGA GATCGGTGGC TGTGCCAACC TCAACGTGCT GTCCTTGCGG 
GACAATCGCC TGACCCTCCT GCCATCGGAG CTGGCCAACA CCACCGAGCT TCATGTTCTG 
GATGTGGCTG GGAACAGGCT GCAGAACTTG CCGTTTGCTC TGACAAACCT CAACCTGAAA 
GCCCTGTGGC TGGCGGAGAA TCAGTCCCAG CCCATGCTGA AATTCCAAAC AGAGGATGAT 
GAGAAGACAG GAGAGAAAGT TCTCACTTGT TACCTGCTTC CTCAGCAGCC CTCTCCCAGC 
CTGGAGAACC TTCTACAGAA CAGCGTGGAC GAGAGCTGGA CGGACACCAA TTTAAACAGA 
GTCAGTGTGA TTCAGTTCCT GGATGAACCC AAAGCAGATG ATGAAGAAGA GTCTGGAGCT 
GAGAGAAGGG GCTTGCAGCG CAGAGCAACC CCCCATCCCA GCGAGCTGAA GGTGATGAAG 
AAGGTGATTG AAGTTCGGCG CAGTGAGGCC CACGCTGCAA AGCCCAATGC AGACCTGAAT 
TCTCCTCACT CAGAAGAGAA GAGGCTGAGC GCCACGTCCA ACCGCAGCGA GGACTCCCAC 
CTTTCCAACA GCACTGCCTC TGCTGACTGT AGGGCGGAGG AGCAGCAGGA GGAGGCAGAG 
AGGAGTCGGC CAAACGGGCA AGTGCCTGAG ATGCAGGAGC TGGAGAAGGA GGCAAAGTCC 
AGAGTGGACG AGGAGCACAA GGAAGCTGCT GGCCATGAAG AGGAAGATGT GGAAGTGGAA 
TACAATGAGC CCACTGTACA CTTTGCTGAA GACACGCTAA TACGGAGTGA GGATGAAGAT 
GAAGATGAAG AGCGACCGTT CCCAGTGGAG AAGCAGCGGC TCATCCGCAA GGACACCCCT 
CACTATAAGA AACACTTTAA GATAACTAAA TTGCCCAAGC CAGAGGCGGT GGTGGCCCTG 
CTGCAGGGGC TGAACAGCGA GGGGGTCCCC AAGGAGGAAG AGGAGTTGGG AGGCTGCAAC 
AACAACACAG AGCCTGCAGA CCAGGAGGAG GTGTGGGATG AGGAGGACCG GAAGAATGGA 
GCTTTGTCTG CCCAGCCATC GGTGAAGGGG GTGTCGTTTG ATCAAGCCAA TAATCTGCTG 
ATTGAACCTG CTCGCATTGA GGAGGAAGAG TTGACTCTGA CTATCGTACG GCAGACGGGG 
GGGCTGGGCA TCAGCATCGC AGGGGGCAAG GGCTCCACCC CCTATAAGGG CGATGATGAG 
GGCATCTTTA TTTCTCGGGT GTCAGAAGAA GGTCCTGCGG CTCGTGCAGG GGTTCGTGTT 
GGGGACAAGC TCCTGGAGGT GAACGGTGTG TCCCTGCACT CCGCTGAGCA CCACGTGGCA 
GTGGAGGCTC TGCGTGGATC GGGAAGTTCC GTCTCCATGA CTGTTCTGCG GGAGCGGATG 
GTGGAGCCGG AGAATGCCAT CACTGTCACC CCGCTGCGCC CCGAGGATGA CTACAGCCCC 
CGTGAGAGGC GAGGTGGCCT GCGCTTCCCG GAGCGACCCG AGGGGACACC TCCCACCGAG 
AGATACTCCA CGTGCCTCAT GCGCAACGAG AAGGGACTGG GCTTCAGTAT CGCTGGTGGC 
AAAGGCTCCA CTCCTTACCG GGCTGGTGAC ATGGGGATCT TTATCTCCCG GATCGCAGAG 
GGTGGTGCAG CCCATCGGGA CGGGATCCTG CACGTAGGAG ATCGGGTCAT CTCGATCAAT 
GGGGTAGACA TGACAGAAGC CAGGCATGAT CAGGCAGTAG CGCTGCTTAC CGCGTCCTCC 
CCCACCATCG TGCTGCTGGT AGAGAGAGAG GGTGCGGAGC AGCCCGGCGA GGGAGACTGG 
CCCGGGGGGC CACGGGCACG CATGCACTCC CCACCGCCAC CCCCCAGCCA TGGGGAGAGC 
CCGCCCGAGG AGACGCCCGC ACCACAGAGG AACCATCTCT CCAAAGGCCT GGAGGATCAG 
TACCCCATTG AGGAAATTCA CCTGGTGAAA GCAGGTGGTC CCCTGGGGCT CAGCATCGTA 
GGGGGCAGTG ACCACTCAAG CCATCCGTTT GGGATTCATG AACCAGGTGT CTTCATTTCG 
AAGGTCATTC CCCGTGGGCT GGCATCCCGC AGTGGGCTGC GAGTAGGGGA CAGGATCCTG 
GAAGTCAATA GCATTGACCT GCGCCACGCT ACCCACCAGG AAGCGGTGAA CGCCCTGCTC 
TCCAACACCC AGGAGCTGAC CATGTTAGTG AGGCGAGACC CACCACCACC TGGTATGCAG 
GAAATATGCA TTGAGAAGGC TCCAGGAGAA AAGCTGGGCA TCAGCATCCG GGGTGGAGCG 
AAAGGCCATG CTGGAAATCC GTTTGATCCC ACTGACGAGG GCATCTTCAT CTCTAAGGTG 
AGCTCCTCGG GGGCGGCAGC CCGGGACGGC CGCCTGAAGG TGGGGATGCG GATCCTAGAG 
GTGAACCACC AGAGCCTGCT GGGGATGACG CACACGGAAG CGGTGCAGAT GCTGCGCGGG 
GTGGGCGACG CGCTCCTGGT GCTGGTGTGT GACGGCTTCG ACCCCAAGGC TGCAGCTGCC 
ATCGAGATGT CCCCGGGAAT TATTGCAAAC CCTTTCGCTG CTGGTATCGG ACGCAAGAAT 
AGCCTGGAAA GTATCTCTTC CATCGACCGG GATTTAAGCC CTGAGGAACT GGAGATCCTG 
CAGAAGGAGA TGGAAATGGT GAGAGAAGCA ACTCAGTGGG AGAGAGAAGA AATGGAGAAA 
GTGGAGCGGA TGCGTAGGGA GCGGGAGGCA GCCACCCGGC AGCTTGAGGA GGAGGCGGAG 
AACGTCACCA GCGAGCCCTT GAGACTGGAC TATCGGACCC TGGCTGCGCT GCCGAGCAGT 
GGTCTCCAGA GAGTCAACCG CACCGCTCCT GCAGGCGAGT CTGGGATGGG CAATTCCAGG 
CGTGAAACCC CCTATTCTCC TGGTAACCAG CAGCGGCCTG CAACCCCTCG GCCCGCTCAC 
CTGCCGGGCA GGGAGACCCG CTTTGCTTCC ATTAACTTCA TTACATCGCA GGATGACAGT 
AAATCGACCA AGCCAGGCGT CATTCAGCCT CTTTCCCGAG TGCGACAAAA TGCTGCCCCG 
CACAATTCGG AGGAGGGGGA CAGCCCCAAT CCCTTCCAGC AGCCCGAGCC GTGCTTCAGT 
GTCCAGAACT CTCCAAAGCC ACCCTCACCA CCCTCTCCTG ATGAGGTCCC CATCAATGTC 
AAGCAAGCGT ACAAGACCTT CGCTGCAGTG CCCTTGTCAC ACCCCCTGCT TGGGACACAG 
GAATTGCCTG GTCAGAGCGG CACAGAGAAT CCCAGAAGTG CCCCGGAGGT GGCTGCGCAC 
AGCCCTGGGG GACTGCACAG CCCTGAGCAG AAGTCCTTCC GTGAGAGGCA GAAGTATTTC 
GAGATTGAGG TGAAGCAGCA GCAGATGGAC AAGCCCCCCA AGAGAGTCTC CTTGGTGGGA 
GAGGACGACC TGAAGAAGAT GAAAGAGGAG GAAGCTCGAA AACTGCAGCA GAAACGTACC 
CTTCTGCTGG AGGAAGAGGC AGAAGAGGAG GAGATGGCGA AGCAGGCGCC TGAGGTGAGC 
GTGCAGTCCA GTGTCATCAT TGAAGGAGTC GAGTACAAGA TTGAGAGACT CAACGGGAGG 
AGCAGCCAGT CTCCGGCCAC TCGGCCCTCC GAGGTCAACG AGAACAGCAG CTCGCAGAGG 
AATTCACTGG AAGAGGGGAT CAAGGCTGAG CAGAGAACCA ACTCCATGTC TGGGCTGAGT 
CCCTTGTACC TTGGAGCAGG GGACGCAGTG GCACCCGTGC GGACGGCCAA AGCTGAACGG 
CGACACCAGG AGCGGTTGCG AATGCAGAGT CCTGAGCTGC TGAGTGGCCA AGAGAAGGAG 
CTTTCTCCTG CAGAACGTCG TGCTCTGGAG GCGGAGAAAC GAGCAATGTG GAGGGCAGCA 
CGATCCTCAG CTCTTGAAGA CAGTATTAAA CAGTACGAGC AGGACCTGGC CAGGAGGCTG 
TACCAGTCCC GACAGCGGGC ACCTGCTCCC GGGGCCGGGC CAGCTCCGAT GCCTAGTCCC 
AAGCAGAGTC ACGCCTCGGC ACCCCCTGGG TCAGGAAGCC ATTCCAGGAT GAAATCGCTT 
GAACAGGATG CTCTGAAAGC CCAGATGGTC ATTGCCAAGT CCAAGGAGGG GAAGAAGCGC 
AGCCCGCTGG AGCAGCTGGC AGAGTCCCCC TCGCCAGTTC CTACACCGTC ACCGACACCA 
CTGGAAGATT GCAGTCCCAG GAACGTCACC TCCCCAGGAA GGCTGTCCAT GTCTGAAAAG 
AAGTTTGACT ACCGGGAATT TGCTGCTATT CCTTCCTCCA AACCCGTTTA CGAAATTCAG 
TCGCCTGATG CTGCTGATGA CCTCAGATAC ATCGAGGACA GGAATAACTC AGTTCCCCAG 
GAGCAGGATG GGCAGCCCGA GGAAGAGGAC ACATTAGGAA CTCACGACTC ATCCACACCC 
ACTCCATCAG CACTTGAAGA AATGGCCCTG TACAGCAGCA AACGCAAGCT CCGACACAGC 
CGGCGCAGCC TGGAGGCTGC CGTCCCTACG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCRIB ( XM_014945727.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014801213.1 Calidris pugnax scribbled planar cell polarity protein (SCRIB), transcript variant X13, mRNA.

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CloneID	OCa67759
Clone ID Related Accession (Same CDS sequence)	XM_014945727.1
Accession Version	XM_014945727.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5502bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	protein scribble homolog isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090871.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCCGC TCTGGCGCTG CAACCGGCAC GTGGAGGCGC TGGACCGGCG GCACTGCTCG 
CTGCAGGCCG TGCCCGAGGA GATCTACCGC TACAGCCGCT CGCTGGAGGA GCTGCTGCTG 
GACGCCAACC AGCTGCGGGA GCTGCCCAAG CCTTTCTTCA GGCTTCTGAA CCTGCGCAAG 
CTGGGCCTGA GTGACAATGA GATCCAAAGA CTTCCCCCTG AGGTGGCCAA CTTCATGCAG 
CTGGTGGAAC TGGACATCTC CCGTAACGAC ATTCCAGAAA TTCCTGAAAG CATCAAATTC 
TGCAAATCCT TGGAAATCGC AGACTTCAGT GGGAATCCTC TCTCCAGGCT TCCCGAGGGC 
TTCACGCAGC TTCGGAGCCT GGGCCATTTG GCCCTGAATG ATGTGTCCCT GCAGAGCCTC 
CCCAGTGACA TTGGGAATTT GGCAAACCTG GTGACTCTGG AGCTACGCGA GAACCTGCTA 
AAGACTCTCC CCACCTCGCT GTCCTTCCTT GTCAAGTTAG AGCAGCTGGA TCTCGGTGGC 
AATGACCTGG AGGTTCTGCC AGACACTCTG GGAGCGCTCC CCAACCTGCG TGAGCTCTGG 
TTAGACCGGA ACCAGCTCTC AGCCCTGCCT CCCGAGCTGG GCAACCTCCG CCGCCTGGTC 
TGCCTGGATG TGTCGGAGAA CAAGCTGGAG CAGTTGCCCA ACGAGGTCAG CGGCCTGGTG 
GCACTGACCG ACTTGCTGCT GTCGCAGAAC CTGTTGGAGT GCATTCCTGA TGGGATTGGT 
CAGCTGAAGC AGCTCTCCAT CCTCAAGGTG GACCAGAATC GACTGACAGA GGTGACAGAG 
TCCATTGGGG ACTGTGAAAA TCTGTCCGAG CTCATCCTGA CAGAGAACAT GCTGACGGCC 
TTGCCGAAAT CCCTTGGGAA GCTGGCCAAG CTGACCAACC TGAACGTGGA TCGGAACCGG 
CTGACATCCC TCCCGGCCGA GATCGGTGGC TGTGCCAACC TCAACGTGCT GTCCTTGCGG 
GACAATCGCC TGACCCTCCT GCCATCGGAG CTGGCCAACA CCACCGAGCT TCATGTTCTG 
GATGTGGCTG GGAACAGGCT GCAGAACTTG CCGTTTGCTC TGACAAACCT CAACCTGAAA 
GCCCTGTGGC TGGCGGAGAA TCAGTCCCAG CCCATGCTGA AATTCCAAAC AGAGGATGAT 
GAGAAGACAG GAGAGAAAGT TCTCACTTGT TACCTGCTTC CTCAGCAGCC CTCTCCCAGC 
CTGGAGAACC TTCTACAGAA CAGCGTGGAC GAGAGCTGGA CGGACACCAA TTTAAACAGA 
GTCAGTGTGA TTCAGTTCCT GGATGAACCC AAAGCAGATG ATGAAGAAGA GTCTGGAGCT 
GAGAGAAGGG GCTTGCAGCG CAGAGCAACC CCCCATCCCA GCGAGCTGAA GGTGATGAAG 
AAGGTGATTG AAGTTCGGCG CAGTGAGGCC CACGCTGCAA AGCCCAATGC AGACCTGAAT 
TCTCCTCACT CAGAAGAGAA GAGGCTGAGC GCCACGTCCA ACCGCAGCGA GGACTCCCAC 
CTTTCCAACA GCACTGCCTC TGCTGACTGT AGGGCGGAGG AGCAGCAGGA GGAGGCAGAG 
AGGAGTCGGC CAAACGGGCA AGTGCCTGAG ATGCAGGAGC TGGAGAAGGA GGCAAAGTCC 
AGAGTGGACG AGGAGCACAA GGAAGCTGCT GGCCATGAAG AGGAAGATGT GGAAGTGGAA 
TACAATGAGC CCACTGTACA CTTTGCTGAA GACACGCTAA TACGGAGTGA GGATGAAGAT 
GAAGATGAAG AGCGACCGTT CCCAGTGGAG AAGCAGCGGC TCATCCGCAA GGACACCCCT 
CACTATAAGA AACACTTTAA GATAACTAAA TTGCCCAAGC CAGAGGCGGT GGTGGCCCTG 
CTGCAGGGGC TGAACAGCGA GGGGGTCCCC AAGGAGGAAG AGGAGTTGGG AGGCTGCAAC 
AACAACACAG AGCCTGCAGA CCAGGAGGAG GTGTGGGATG AGGAGGACCG GAAGAATGGA 
GCTTTGTCTG CCCAGCCATC GGTGAAGGGG GTGTCGTTTG ATCAAGCCAA TAATCTGCTG 
ATTGAACCTG CTCGCATTGA GGAGGAAGAG TTGACTCTGA CTATCGTACG GCAGACGGGG 
GGGCTGGGCA TCAGCATCGC AGGGGGCAAG GGCTCCACCC CCTATAAGGG CGATGATGAG 
GGCATCTTTA TTTCTCGGGT GTCAGAAGAA GGTCCTGCGG CTCGTGCAGG GGTTCGTGTT 
GGGGACAAGC TCCTGGAGGT GAACGGTGTG TCCCTGCACT CCGCTGAGCA CCACGTGGCA 
GTGGAGGCTC TGCGTGGATC GGGAAGTTCC GTCTCCATGA CTGTTCTGCG GGAGCGGATG 
GTGGAGCCGG AGAATGCCAT CACTGTCACC CCGCTGCGCC CCGAGGATGA CTACAGCCCC 
CGTGAGAGGC GAGGTGGCCT GCGCTTCCCG GAGCGACCCG AGGGGACACC TCCCACCGAG 
AGATACTCCA CGTGCCTCAT GCGCAACGAG AAGGGACTGG GCTTCAGTAT CGCTGGTGGC 
AAAGGCTCCA CTCCTTACCG GGCTGGTGAC ATGGGGATCT TTATCTCCCG GATCGCAGAG 
GGTGGTGCAG CCCATCGGGA CGGGATCCTG CACGTAGGAG ATCGGGTCAT CTCGATCAAT 
GGGGTAGACA TGACAGAAGC CAGGCATGAT CAGGCAGTAG CGCTGCTTAC CGCGTCCTCC 
CCCACCATCG TGCTGCTGGT AGAGAGAGAG GGTGCGGAGC AGCCCGGCGA GGGAGACTGG 
CCCGGGGGGC CACGGGCACG CATGCACTCC CCACCGCCAC CCCCCAGCCA TGGGGAGAGC 
CCGCCCGAGG AGACGCCCGC ACCACAGAGG AACCATCTCT CCAAAGGCCT GGAGGATCAG 
TACCCCATTG AGGAAATTCA CCTGGTGAAA GCAGGTGGTC CCCTGGGGCT CAGCATCGTA 
GGGGGCAGTG ACCACTCAAG CCATCCGTTT GGGATTCATG AACCAGGTGT CTTCATTTCG 
AAGGTCATTC CCCGTGGGCT GGCATCCCGC AGTGGGCTGC GAGTAGGGGA CAGGATCCTG 
GAAGTCAATA GCATTGACCT GCGCCACGCT ACCCACCAGG AAGCGGTGAA CGCCCTGCTC 
TCCAACACCC AGGAGCTGAC CATGTTAGTG AGGCGAGACC CACCACCACC TGGTATGCAG 
GAAATATGCA TTGAGAAGGC TCCAGGAGAA AAGCTGGGCA TCAGCATCCG GGGTGGAGCG 
AAAGGCCATG CTGGAAATCC GTTTGATCCC ACTGACGAGG GCATCTTCAT CTCTAAGGTG 
AGCTCCTCGG GGGCGGCAGC CCGGGACGGC CGCCTGAAGG TGGGGATGCG GATCCTAGAG 
GTGAACCACC AGAGCCTGCT GGGGATGACG CACACGGAAG CGGTGCAGAT GCTGCGCGGG 
GTGGGCGACG CGCTCCTGGT GCTGGTGTGT GACGGCTTCG ACCCCAAGGC TGCAGCTGCC 
ATCGAGATGT CCCCGGGAAT TATTGCAAAC CCTTTCGCTG CTGGTATCGG ACGCAAGAAT 
AGCCTGGAAA GTATCTCTTC CATCGACCGG GATTTAAGCC CTGAGGAACT GGAGATCCTG 
CAGAAGGAGA TGGAAATGGT GAGAGAAGCA ACTCAGTGGG AGAGAGAAGA AATGGAGAAA 
GTGGAGCGGA TGCGTAGGGA GCGGGAGGCA GCCACCCGGC AGCTTGAGGA GGAGGCGGAG 
AACGTCACCA GCGAGCCCTT GAGACTGGAC TATCGGACCC TGGCTGCGCT GCCGAGCAGT 
GGTCTCCAGA GAGTCAACCG CACCGCTCCT GCAGGCGAGT CTGGGATGGG CAATTCCAGG 
CGTGAAACCC CCTATTCTCC TGGTAACCAG CAGGCTTCCA TTAACTTCAT TACATCGCAG 
GATGACAGTA AATCGACCAA GCCAGGCGTC ATTCAGCCTC TTTCCCGAGT GCGACAAAAT 
GCTGCCCCGC ACAATTCGGA GGAGGGGGAC AGCCCCAATC CCTTCCAGCA GCCCGAGCCG 
TGCTTCAGTG TCCAGAACTC TCCAAAGCCA CCCTCACCAC CCTCTCCTGA TGAGGTCCCC 
ATCAATGTCA AGCAAGCGTA CAAGACCTTC GCTGCAGTGC CCTTGTCACA CCCCCTGCTT 
GGGACACAGG AATTGCCTGG TCAGAGCGGC ACAGAGAATC CCAGAAGTGC CCCGGAGGTG 
GCTGCGCACA GCCCTGGGGG ACTGCACAGC CCTGAGCAGA AGTCCTTCCG TGAGAGGCAG 
AAGTATTTCG AGATTGAGGT GAAGCAGCAG CAGATGGACA AGCCCCCCAA GAGAGTCTCC 
TTGGTGGGAG AGGACGACCT GAAGAAGATG AAAGAGGAGG AAGCTCGAAA ACTGCAGCAG 
AAACGTACCC TTCTGCTGGA GGAAGAGGCA GAAGAGGAGG AGATGGCGAA GCAGGCGCCT 
GAGGTGAGCG TGCAGTCCAG TGTCATCATT GAAGGAGTCG AGTACAAGAT TGAGAGACTC 
AACGGGAGGA GCAGCCAGTC TCCGGCCACT CGGCCCTCCG AGGTCAACGA GAACAGCAGC 
TCGCAGAGGA ATTCACTGGA AGAGGGGATC AAGGCTGAGC AGAGAACCAA CTCCATGTCT 
GGGCTGAGTC CCTTGTACCT TGGAGCAGGG GACGCAGTGG CACCCGTGCG GACGGCCAAA 
GCTGAACGGC GACACCAGGA GCGGTTGCGA ATGCAGAGTC CTGAGCTGCT GAGTGGCCAA 
GAGAAGGAGC TTTCTCCTGC AGAACGTCGT GCTCTGGAGG CGGAGAAACG AGCAATGTGG 
AGGGCAGCAC GATCCTCAGC TCTTGAAGAC AGTATTAAAC AGTACGAGCA GGACCTGGCC 
AGGAGGCTGT ACCAGTCCCG ACAGCGGGCA CCTGCTCCCG GGGCCGGGCC AGCTCCGATG 
CCTAGTCCCA AGCAGAGTCA CGCCTCGGCA CCCCCTGGGT CAGGAAGCCA TTCCAGGATG 
AAATCGCTTG AACAGGATGC TCTGAAAGCC CAGATGGTCA TTGCCAAGTC CAAGGAGGGG 
AAGAAGCGCA GCCCGCTGGA GCAGCTGGCA GAGTCCCCCT CGCCAGTTCC TACACCGTCA 
CCGACACCAC TGGAAGATTG CAGTCCCAGG AACGTCACCT CCCCAGGAAG GCTGTCCATG 
TCTGAAAAGA AGTTTGACTA CCGGGAATTT GCTGCTATTC CTTCCTCCAA ACCCGTTTAC 
GAAATTCAGT CGCCTGATGC TGCTGATGAC CTCAGATACA TCGAGGACAG GAATAACTCA 
GTTCCCCAGG AGCAGGATGG GCAGCCCGAG GAAGAGGACA CATTAGGAAC TCACGACTCA 
TCCACACCCA CTCCATCAGC ACTTGAAGAA ATGGCCCTGT ACAGCAGCAA ACGCAAGCTC 
CGACACAGCC GGCGCAGCCT GGAGGCTGCC GTCCCTACGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014801213.1
CDS	6..5507
Translation	MPPLWRCNRHVEALDRRHCSLQAVPEEIYRYSRSLEELLLDANQLRELPKPFFRLLNLRKLGLSDNEIQRLPPEVANFMQLVELDISRNDIPEIPESIKFCKSLEIADFSGNPLSRLPEGFTQLRSLGHLALNDVSLQSLPSDIGNLANLVTLELRENLLKTLPTSLSFLVKLEQLDLGGNDLEVLPDTLGALPNLRELWLDRNQLSALPPELGNLRRLVCLDVSENKLEQLPNEVSGLVALTDLLLSQNLLECIPDGIGQLKQLSILKVDQNRLTEVTESIGDCENLSELILTENMLTALPKSLGKLAKLTNLNVDRNRLTSLPAEIGGCANLNVLSLRDNRLTLLPSELANTTELHVLDVAGNRLQNLPFALTNLNLKALWLAENQSQPMLKFQTEDDEKTGEKVLTCYLLPQQPSPSLENLLQNSVDESWTDTNLNRVSVIQFLDEPKADDEEESGAERRGLQRRATPHPSELKVMKKVIEVRRSEAHAAKPNADLNSPHSEEKRLSATSNRSEDSHLSNSTASADCRAEEQQEEAERSRPNGQVPEMQELEKEAKSRVDEEHKEAAGHEEEDVEVEYNEPTVHFAEDTLIRSEDEDEDEERPFPVEKQRLIRKDTPHYKKHFKITKLPKPEAVVALLQGLNSEGVPKEEEELGGCNNNTEPADQEEVWDEEDRKNGALSAQPSVKGVSFDQANNLLIEPARIEEEELTLTIVRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVRVGDKLLEVNGVSLHSAEHHVAVEALRGSGSSVSMTVLRERMVEPENAITVTPLRPEDDYSPRERRGGLRFPERPEGTPPTERYSTCLMRNEKGLGFSIAGGKGSTPYRAGDMGIFISRIAEGGAAHRDGILHVGDRVISINGVDMTEARHDQAVALLTASSPTIVLLVEREGAEQPGEGDWPGGPRARMHSPPPPPSHGESPPEETPAPQRNHLSKGLEDQYPIEEIHLVKAGGPLGLSIVGGSDHSSHPFGIHEPGVFISKVIPRGLASRSGLRVGDRILEVNSIDLRHATHQEAVNALLSNTQELTMLVRRDPPPPGMQEICIEKAPGEKLGISIRGGAKGHAGNPFDPTDEGIFISKVSSSGAAARDGRLKVGMRILEVNHQSLLGMTHTEAVQMLRGVGDALLVLVCDGFDPKAAAAIEMSPGIIANPFAAGIGRKNSLESISSIDRDLSPEELEILQKEMEMVREATQWEREEMEKVERMRREREAATRQLEEEAENVTSEPLRLDYRTLAALPSSGLQRVNRTAPAGESGMGNSRRETPYSPGNQQASINFITSQDDSKSTKPGVIQPLSRVRQNAAPHNSEEGDSPNPFQQPEPCFSVQNSPKPPSPPSPDEVPINVKQAYKTFAAVPLSHPLLGTQELPGQSGTENPRSAPEVAAHSPGGLHSPEQKSFRERQKYFEIEVKQQQMDKPPKRVSLVGEDDLKKMKEEEARKLQQKRTLLLEEEAEEEEMAKQAPEVSVQSSVIIEGVEYKIERLNGRSSQSPATRPSEVNENSSSQRNSLEEGIKAEQRTNSMSGLSPLYLGAGDAVAPVRTAKAERRHQERLRMQSPELLSGQEKELSPAERRALEAEKRAMWRAARSSALEDSIKQYEQDLARRLYQSRQRAPAPGAGPAPMPSPKQSHASAPPGSGSHSRMKSLEQDALKAQMVIAKSKEGKKRSPLEQLAESPSPVPTPSPTPLEDCSPRNVTSPGRLSMSEKKFDYREFAAIPSSKPVYEIQSPDAADDLRYIEDRNNSVPQEQDGQPEEEDTLGTHDSSTPTPSALEEMALYSSKRKLRHSRRSLEAAVPT

Target ORF information:

RefSeq Version	XM_014945727.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax scribbled planar cell polarity protein (SCRIB), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014945727.1

ORF Insert Sequence:

ATGCCGCCGC TCTGGCGCTG CAACCGGCAC GTGGAGGCGC TGGACCGGCG GCACTGCTCG 
CTGCAGGCCG TGCCCGAGGA GATCTACCGC TACAGCCGCT CGCTGGAGGA GCTGCTGCTG 
GACGCCAACC AGCTGCGGGA GCTGCCCAAG CCTTTCTTCA GGCTTCTGAA CCTGCGCAAG 
CTGGGCCTGA GTGACAATGA GATCCAAAGA CTTCCCCCTG AGGTGGCCAA CTTCATGCAG 
CTGGTGGAAC TGGACATCTC CCGTAACGAC ATTCCAGAAA TTCCTGAAAG CATCAAATTC 
TGCAAATCCT TGGAAATCGC AGACTTCAGT GGGAATCCTC TCTCCAGGCT TCCCGAGGGC 
TTCACGCAGC TTCGGAGCCT GGGCCATTTG GCCCTGAATG ATGTGTCCCT GCAGAGCCTC 
CCCAGTGACA TTGGGAATTT GGCAAACCTG GTGACTCTGG AGCTACGCGA GAACCTGCTA 
AAGACTCTCC CCACCTCGCT GTCCTTCCTT GTCAAGTTAG AGCAGCTGGA TCTCGGTGGC 
AATGACCTGG AGGTTCTGCC AGACACTCTG GGAGCGCTCC CCAACCTGCG TGAGCTCTGG 
TTAGACCGGA ACCAGCTCTC AGCCCTGCCT CCCGAGCTGG GCAACCTCCG CCGCCTGGTC 
TGCCTGGATG TGTCGGAGAA CAAGCTGGAG CAGTTGCCCA ACGAGGTCAG CGGCCTGGTG 
GCACTGACCG ACTTGCTGCT GTCGCAGAAC CTGTTGGAGT GCATTCCTGA TGGGATTGGT 
CAGCTGAAGC AGCTCTCCAT CCTCAAGGTG GACCAGAATC GACTGACAGA GGTGACAGAG 
TCCATTGGGG ACTGTGAAAA TCTGTCCGAG CTCATCCTGA CAGAGAACAT GCTGACGGCC 
TTGCCGAAAT CCCTTGGGAA GCTGGCCAAG CTGACCAACC TGAACGTGGA TCGGAACCGG 
CTGACATCCC TCCCGGCCGA GATCGGTGGC TGTGCCAACC TCAACGTGCT GTCCTTGCGG 
GACAATCGCC TGACCCTCCT GCCATCGGAG CTGGCCAACA CCACCGAGCT TCATGTTCTG 
GATGTGGCTG GGAACAGGCT GCAGAACTTG CCGTTTGCTC TGACAAACCT CAACCTGAAA 
GCCCTGTGGC TGGCGGAGAA TCAGTCCCAG CCCATGCTGA AATTCCAAAC AGAGGATGAT 
GAGAAGACAG GAGAGAAAGT TCTCACTTGT TACCTGCTTC CTCAGCAGCC CTCTCCCAGC 
CTGGAGAACC TTCTACAGAA CAGCGTGGAC GAGAGCTGGA CGGACACCAA TTTAAACAGA 
GTCAGTGTGA TTCAGTTCCT GGATGAACCC AAAGCAGATG ATGAAGAAGA GTCTGGAGCT 
GAGAGAAGGG GCTTGCAGCG CAGAGCAACC CCCCATCCCA GCGAGCTGAA GGTGATGAAG 
AAGGTGATTG AAGTTCGGCG CAGTGAGGCC CACGCTGCAA AGCCCAATGC AGACCTGAAT 
TCTCCTCACT CAGAAGAGAA GAGGCTGAGC GCCACGTCCA ACCGCAGCGA GGACTCCCAC 
CTTTCCAACA GCACTGCCTC TGCTGACTGT AGGGCGGAGG AGCAGCAGGA GGAGGCAGAG 
AGGAGTCGGC CAAACGGGCA AGTGCCTGAG ATGCAGGAGC TGGAGAAGGA GGCAAAGTCC 
AGAGTGGACG AGGAGCACAA GGAAGCTGCT GGCCATGAAG AGGAAGATGT GGAAGTGGAA 
TACAATGAGC CCACTGTACA CTTTGCTGAA GACACGCTAA TACGGAGTGA GGATGAAGAT 
GAAGATGAAG AGCGACCGTT CCCAGTGGAG AAGCAGCGGC TCATCCGCAA GGACACCCCT 
CACTATAAGA AACACTTTAA GATAACTAAA TTGCCCAAGC CAGAGGCGGT GGTGGCCCTG 
CTGCAGGGGC TGAACAGCGA GGGGGTCCCC AAGGAGGAAG AGGAGTTGGG AGGCTGCAAC 
AACAACACAG AGCCTGCAGA CCAGGAGGAG GTGTGGGATG AGGAGGACCG GAAGAATGGA 
GCTTTGTCTG CCCAGCCATC GGTGAAGGGG GTGTCGTTTG ATCAAGCCAA TAATCTGCTG 
ATTGAACCTG CTCGCATTGA GGAGGAAGAG TTGACTCTGA CTATCGTACG GCAGACGGGG 
GGGCTGGGCA TCAGCATCGC AGGGGGCAAG GGCTCCACCC CCTATAAGGG CGATGATGAG 
GGCATCTTTA TTTCTCGGGT GTCAGAAGAA GGTCCTGCGG CTCGTGCAGG GGTTCGTGTT 
GGGGACAAGC TCCTGGAGGT GAACGGTGTG TCCCTGCACT CCGCTGAGCA CCACGTGGCA 
GTGGAGGCTC TGCGTGGATC GGGAAGTTCC GTCTCCATGA CTGTTCTGCG GGAGCGGATG 
GTGGAGCCGG AGAATGCCAT CACTGTCACC CCGCTGCGCC CCGAGGATGA CTACAGCCCC 
CGTGAGAGGC GAGGTGGCCT GCGCTTCCCG GAGCGACCCG AGGGGACACC TCCCACCGAG 
AGATACTCCA CGTGCCTCAT GCGCAACGAG AAGGGACTGG GCTTCAGTAT CGCTGGTGGC 
AAAGGCTCCA CTCCTTACCG GGCTGGTGAC ATGGGGATCT TTATCTCCCG GATCGCAGAG 
GGTGGTGCAG CCCATCGGGA CGGGATCCTG CACGTAGGAG ATCGGGTCAT CTCGATCAAT 
GGGGTAGACA TGACAGAAGC CAGGCATGAT CAGGCAGTAG CGCTGCTTAC CGCGTCCTCC 
CCCACCATCG TGCTGCTGGT AGAGAGAGAG GGTGCGGAGC AGCCCGGCGA GGGAGACTGG 
CCCGGGGGGC CACGGGCACG CATGCACTCC CCACCGCCAC CCCCCAGCCA TGGGGAGAGC 
CCGCCCGAGG AGACGCCCGC ACCACAGAGG AACCATCTCT CCAAAGGCCT GGAGGATCAG 
TACCCCATTG AGGAAATTCA CCTGGTGAAA GCAGGTGGTC CCCTGGGGCT CAGCATCGTA 
GGGGGCAGTG ACCACTCAAG CCATCCGTTT GGGATTCATG AACCAGGTGT CTTCATTTCG 
AAGGTCATTC CCCGTGGGCT GGCATCCCGC AGTGGGCTGC GAGTAGGGGA CAGGATCCTG 
GAAGTCAATA GCATTGACCT GCGCCACGCT ACCCACCAGG AAGCGGTGAA CGCCCTGCTC 
TCCAACACCC AGGAGCTGAC CATGTTAGTG AGGCGAGACC CACCACCACC TGGTATGCAG 
GAAATATGCA TTGAGAAGGC TCCAGGAGAA AAGCTGGGCA TCAGCATCCG GGGTGGAGCG 
AAAGGCCATG CTGGAAATCC GTTTGATCCC ACTGACGAGG GCATCTTCAT CTCTAAGGTG 
AGCTCCTCGG GGGCGGCAGC CCGGGACGGC CGCCTGAAGG TGGGGATGCG GATCCTAGAG 
GTGAACCACC AGAGCCTGCT GGGGATGACG CACACGGAAG CGGTGCAGAT GCTGCGCGGG 
GTGGGCGACG CGCTCCTGGT GCTGGTGTGT GACGGCTTCG ACCCCAAGGC TGCAGCTGCC 
ATCGAGATGT CCCCGGGAAT TATTGCAAAC CCTTTCGCTG CTGGTATCGG ACGCAAGAAT 
AGCCTGGAAA GTATCTCTTC CATCGACCGG GATTTAAGCC CTGAGGAACT GGAGATCCTG 
CAGAAGGAGA TGGAAATGGT GAGAGAAGCA ACTCAGTGGG AGAGAGAAGA AATGGAGAAA 
GTGGAGCGGA TGCGTAGGGA GCGGGAGGCA GCCACCCGGC AGCTTGAGGA GGAGGCGGAG 
AACGTCACCA GCGAGCCCTT GAGACTGGAC TATCGGACCC TGGCTGCGCT GCCGAGCAGT 
GGTCTCCAGA GAGTCAACCG CACCGCTCCT GCAGGCGAGT CTGGGATGGG CAATTCCAGG 
CGTGAAACCC CCTATTCTCC TGGTAACCAG CAGGCTTCCA TTAACTTCAT TACATCGCAG 
GATGACAGTA AATCGACCAA GCCAGGCGTC ATTCAGCCTC TTTCCCGAGT GCGACAAAAT 
GCTGCCCCGC ACAATTCGGA GGAGGGGGAC AGCCCCAATC CCTTCCAGCA GCCCGAGCCG 
TGCTTCAGTG TCCAGAACTC TCCAAAGCCA CCCTCACCAC CCTCTCCTGA TGAGGTCCCC 
ATCAATGTCA AGCAAGCGTA CAAGACCTTC GCTGCAGTGC CCTTGTCACA CCCCCTGCTT 
GGGACACAGG AATTGCCTGG TCAGAGCGGC ACAGAGAATC CCAGAAGTGC CCCGGAGGTG 
GCTGCGCACA GCCCTGGGGG ACTGCACAGC CCTGAGCAGA AGTCCTTCCG TGAGAGGCAG 
AAGTATTTCG AGATTGAGGT GAAGCAGCAG CAGATGGACA AGCCCCCCAA GAGAGTCTCC 
TTGGTGGGAG AGGACGACCT GAAGAAGATG AAAGAGGAGG AAGCTCGAAA ACTGCAGCAG 
AAACGTACCC TTCTGCTGGA GGAAGAGGCA GAAGAGGAGG AGATGGCGAA GCAGGCGCCT 
GAGGTGAGCG TGCAGTCCAG TGTCATCATT GAAGGAGTCG AGTACAAGAT TGAGAGACTC 
AACGGGAGGA GCAGCCAGTC TCCGGCCACT CGGCCCTCCG AGGTCAACGA GAACAGCAGC 
TCGCAGAGGA ATTCACTGGA AGAGGGGATC AAGGCTGAGC AGAGAACCAA CTCCATGTCT 
GGGCTGAGTC CCTTGTACCT TGGAGCAGGG GACGCAGTGG CACCCGTGCG GACGGCCAAA 
GCTGAACGGC GACACCAGGA GCGGTTGCGA ATGCAGAGTC CTGAGCTGCT GAGTGGCCAA 
GAGAAGGAGC TTTCTCCTGC AGAACGTCGT GCTCTGGAGG CGGAGAAACG AGCAATGTGG 
AGGGCAGCAC GATCCTCAGC TCTTGAAGAC AGTATTAAAC AGTACGAGCA GGACCTGGCC 
AGGAGGCTGT ACCAGTCCCG ACAGCGGGCA CCTGCTCCCG GGGCCGGGCC AGCTCCGATG 
CCTAGTCCCA AGCAGAGTCA CGCCTCGGCA CCCCCTGGGT CAGGAAGCCA TTCCAGGATG 
AAATCGCTTG AACAGGATGC TCTGAAAGCC CAGATGGTCA TTGCCAAGTC CAAGGAGGGG 
AAGAAGCGCA GCCCGCTGGA GCAGCTGGCA GAGTCCCCCT CGCCAGTTCC TACACCGTCA 
CCGACACCAC TGGAAGATTG CAGTCCCAGG AACGTCACCT CCCCAGGAAG GCTGTCCATG 
TCTGAAAAGA AGTTTGACTA CCGGGAATTT GCTGCTATTC CTTCCTCCAA ACCCGTTTAC 
GAAATTCAGT CGCCTGATGC TGCTGATGAC CTCAGATACA TCGAGGACAG GAATAACTCA 
GTTCCCCAGG AGCAGGATGG GCAGCCCGAG GAAGAGGACA CATTAGGAAC TCACGACTCA 
TCCACACCCA CTCCATCAGC ACTTGAAGAA ATGGCCCTGT ACAGCAGCAA ACGCAAGCTC 
CGACACAGCC GGCGCAGCCT GGAGGCTGCC GTCCCTACGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCRIB ( XM_014945728.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014801214.1 Calidris pugnax scribbled planar cell polarity protein (SCRIB), transcript variant X14, mRNA.

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CloneID	OCa67760
Clone ID Related Accession (Same CDS sequence)	XM_014945728.1
Accession Version	XM_014945728.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5496bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	protein scribble homolog isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090871.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCCGC TCTGGCGCTG CAACCGGCAC GTGGAGGCGC TGGACCGGCG GCACTGCTCG 
CTGCAGGCCG TGCCCGAGGA GATCTACCGC TACAGCCGCT CGCTGGAGGA GCTGCTGCTG 
GACGCCAACC AGCTGCGGGA GCTGCCCAAG CCTTTCTTCA GGCTTCTGAA CCTGCGCAAG 
CTGGGCCTGA GTGACAATGA GATCCAAAGA CTTCCCCCTG AGGTGGCCAA CTTCATGCAG 
CTGGTGGAAC TGGACATCTC CCGTAACGAC ATTCCAGAAA TTCCTGAAAG CATCAAATTC 
TGCAAATCCT TGGAAATCGC AGACTTCAGT GGGAATCCTC TCTCCAGGCT TCCCGAGGGC 
TTCACGCAGC TTCGGAGCCT GGGCCATTTG GCCCTGAATG ATGTGTCCCT GCAGAGCCTC 
CCCAGTGACA TTGGGAATTT GGCAAACCTG GTGACTCTGG AGCTACGCGA GAACCTGCTA 
AAGACTCTCC CCACCTCGCT GTCCTTCCTT GTCAAGTTAG AGCAGCTGGA TCTCGGTGGC 
AATGACCTGG AGGTTCTGCC AGACACTCTG GGAGCGCTCC CCAACCTGCG TGAGCTCTGG 
TTAGACCGGA ACCAGCTCTC AGCCCTGCCT CCCGAGCTGG GCAACCTCCG CCGCCTGGTC 
TGCCTGGATG TGTCGGAGAA CAAGCTGGAG CAGTTGCCCA ACGAGGTCAG CGGCCTGGTG 
GCACTGACCG ACTTGCTGCT GTCGCAGAAC CTGTTGGAGT GCATTCCTGA TGGGATTGGT 
CAGCTGAAGC AGCTCTCCAT CCTCAAGGTG GACCAGAATC GACTGACAGA GGTGACAGAG 
TCCATTGGGG ACTGTGAAAA TCTGTCCGAG CTCATCCTGA CAGAGAACAT GCTGACGGCC 
TTGCCGAAAT CCCTTGGGAA GCTGGCCAAG CTGACCAACC TGAACGTGGA TCGGAACCGG 
CTGACATCCC TCCCGGCCGA GATCGGTGGC TGTGCCAACC TCAACGTGCT GTCCTTGCGG 
GACAATCGCC TGACCCTCCT GCCATCGGAG CTGGCCAACA CCACCGAGCT TCATGTTCTG 
GATGTGGCTG GGAACAGGCT GCAGAACTTG CCGTTTGCTC TGACAAACCT CAACCTGAAA 
GCCCTGTGGC TGGCGGAGAA TCAGTCCCAG CCCATGCTGA AATTCCAAAC AGAGGATGAT 
GAGAAGACAG GAGAGAAAGT TCTCACTTGT TACCTGCTTC CTCAGCAGCC CTCTCCCAGC 
CTGGAGAACC TTCTACAGAA CAGCGTGGAC GAGAGCTGGA CGGACACCAA TTTAAACAGA 
GTCAGTGTGA TTCAGTTCCT GGATGAACCC AAAGCAGATG ATGAAGAAGA GTCTGGAGCT 
GAGAGAAGGG GCTTGCAGCG CAGAGCAACC CCCCATCCCA GCGAGCTGAA GGTGATGAAG 
AAGGTGATTG AAGTTCGGCG CAGTGAGGCC CACGCTGCAA AGCCCAATGC AGACCTGAAT 
TCTCCTCACT CAGAAGAGAA GAGGCTGAGC GCCACGTCCA ACCGCAGCGA GGACTCCCAC 
CTTTCCAACA GCACTGCCTC TGCTGACTGT AGGGCGGAGG AGCAGCAGGA GGAGGCAGAG 
AGGAGTCGGC CAAACGGGCA AGTGCCTGAG ATGCAGGAGC TGGAGAAGGA GGCAAAGTCC 
AGAGTGGACG AGGAGCACAA GGAAGCTGCT GGCCATGAAG AGGAAGATGT GGAAGTGGAA 
TACAATGAGC CCACTGTACA CTTTGCTGAA GACACGCTAA TACGGAGTGA GGATGAAGAT 
GAAGATGAAG AGCGACCGTT CCCAGTGGAG AAGCAGCGGC TCATCCGCAA GGACACCCCT 
CACTATAAGA AACACTTTAA GATAACTAAA TTGCCCAAGC CAGAGGCGGT GGTGGCCCTG 
CTGCAGGGGC TGAACAGCGA GGGGGTCCCC AAGGAGGAAG AGGAGTTGGG AGGCTGCAAC 
AACAACACAG AGCCTGCAGA CCAGGAGGAG GTGTGGGATG AGGAGGACCG GAAGAATGGA 
GCTTTGTCTG CCCAGCCATC GGTGAAGGGG GTGTCGTTTG ATCAAGCCAA TAATCTGCTG 
ATTGAACCTG CTCGCATTGA GGAGGAAGAG TTGACTCTGA CTATCGTACG GCAGACGGGG 
GGGCTGGGCA TCAGCATCGC AGGGGGCAAG GGCTCCACCC CCTATAAGGG CGATGATGAG 
GGCATCTTTA TTTCTCGGGT GTCAGAAGAA GGTCCTGCGG CTCGTGCAGG GGTTCGTGTT 
GGGGACAAGC TCCTGGAGGT GAACGGTGTG TCCCTGCACT CCGCTGAGCA CCACGTGGCA 
GTGGAGGCTC TGCGTGGATC GGGAAGTTCC GTCTCCATGA CTGTTCTGCG GGAGCGGATG 
GTGGAGCCGG AGAATGCCAT CACTGTCACC CCGCTGCGCC CCGAGGATGA CTACAGCCCC 
CGTGAGAGGC GAGGTGGCCT GCGCTTCCCG GAGCGACCCG AGGGGACACC TCCCACCGAG 
AGATACTCCA CGTGCCTCAT GCGCAACGAG AAGGGACTGG GCTTCAGTAT CGCTGGTGGC 
AAAGGCTCCA CTCCTTACCG GGCTGGTGAC ATGGGGATCT TTATCTCCCG GATCGCAGAG 
GGTGGTGCAG CCCATCGGGA CGGGATCCTG CACGTAGGAG ATCGGGTCAT CTCGATCAAT 
GGGGTAGACA TGACAGAAGC CAGGCATGAT CAGGCAGTAG CGCTGCTTAC CGCGTCCTCC 
CCCACCATCG TGCTGCTGGT AGAGAGAGAG GGTGCGGAGC AGCCCGGCGA GGGAGACTGG 
CCCGGGGGGC CACGGGCACG CATGCACTCC CCACCGCCAC CCCCCAGCCA TGGGGAGAGC 
CCGCCCGAGG AGACGCCCGC ACCACAGAGG AACCATCTCT CCAAAGGCCT GGAGGATCAG 
TACCCCATTG AGGAAATTCA CCTGGTGAAA GCAGGTGGTC CCCTGGGGCT CAGCATCGTA 
GGGGGCAGTG ACCACTCAAG CCATCCGTTT GGGATTCATG AACCAGGTGT CTTCATTTCG 
AAGGTCATTC CCCGTGGGCT GGCATCCCGC AGTGGGCTGC GAGTAGGGGA CAGGATCCTG 
GAAGTCAATA GCATTGACCT GCGCCACGCT ACCCACCAGG AAGCGGTGAA CGCCCTGCTC 
TCCAACACCC AGGAGCTGAC CATGTTAGTG AGGCGAGACC CACCACCACC TGGTATGCAG 
GAAATATGCA TTGAGAAGGC TCCAGGAGAA AAGCTGGGCA TCAGCATCCG GGGTGGAGCG 
AAAGGCCATG CTGGAAATCC GTTTGATCCC ACTGACGAGG GCATCTTCAT CTCTAAGGTG 
AGCTCCTCGG GGGCGGCAGC CCGGGACGGC CGCCTGAAGG TGGGGATGCG GATCCTAGAG 
GTGAACCACC AGAGCCTGCT GGGGATGACG CACACGGAAG CGGTGCAGAT GCTGCGCGGG 
GTGGGCGACG CGCTCCTGGT GCTGGTGTGT GACGGCTTCG ACCCCAAGGC TGCAGCTGCC 
ATCGAGATGT CCCCGGGAAT TATTGCAAAC CCTTTCGCTG CTGGTATCGG ACGCAAGAAT 
AGCCTGGAAA GTATCTCTTC CATCGACCGG GATTTAAGCC CTGAGGAACT GGAGATCCTG 
CAGAAGGAGA TGGAAATGGT GAGAGAAGCA ACTCAGTGGG AGAGAGAAGA AATGGAGAAA 
GTGAACGTCA CCAGCGAGCC CTTGAGACTG GACTATCGGA CCCTGGCTGC GCTGCCGAGC 
AGTGGTCTCC AGAGAGTCAA CCGCACCGCT CCTGCAGGCG AGTCTGGGAT GGGCAATTCC 
AGGCGTGAAA CCCCCTATTC TCCTGGTAAC CAGCAGCGGC CTGCAACCCC TCGGCCCGCT 
CACCTGCCGG GCAGGGAGAC CCGCTTTGCT TCCATTAACT TCATTACATC GCAGGATGAC 
AGTAAATCGA CCAAGCCAGG CGTCATTCAG CCTCTTTCCC GAGTGCGACA AAATGCTGCC 
CCGCACAATT CGGAGGAGGG GGACAGCCCC AATCCCTTCC AGCAGCCCGA GCCGTGCTTC 
AGTGTCCAGA ACTCTCCAAA GCCACCCTCA CCACCCTCTC CTGATGAGGT CCCCATCAAT 
GTCAAGCAAG CGTACAAGAC CTTCGCTGCA GTGCCCTTGT CACACCCCCT GCTTGGGACA 
CAGGAATTGC CTGGTCAGAG CGGCACAGAG AATCCCAGAA GTGCCCCGGA GGTGGCTGCG 
CACAGCCCTG GGGGACTGCA CAGCCCTGAG CAGAAGTCCT TCCGTGAGAG GCAGAAGTAT 
TTCGAGATTG AGGTGAAGCA GCAGCAGATG GACAAGCCCC CCAAGAGAGT CTCCTTGGTG 
GGAGAGGACG ACCTGAAGAA GATGAAAGAG GAGGAAGCTC GAAAACTGCA GCAGAAACGT 
ACCCTTCTGC TGGAGGAAGA GGCAGAAGAG GAGGAGATGG CGAAGCAGGC GCCTGAGGTG 
AGCGTGCAGT CCAGTGTCAT CATTGAAGGA GTCGAGTACA AGATTGAGAG ACTCAACGGG 
AGGAGCAGCC AGTCTCCGGC CACTCGGCCC TCCGAGGTCA ACGAGAACAG CAGCTCGCAG 
AGGAATTCAC TGGAAGAGGG GATCAAGGCT GAGCAGAGAA CCAACTCCAT GTCTGGGCTG 
AGTCCCTTGT ACCTTGGAGC AGGGGACGCA GTGGCACCCG TGCGGACGGC CAAAGCTGAA 
CGGCGACACC AGGAGCGGTT GCGAATGCAG AGTCCTGAGC TGCTGAGTGG CCAAGAGAAG 
GAGCTTTCTC CTGCAGAACG TCGTGCTCTG GAGGCGGAGA AACGAGCAAT GTGGAGGGCA 
GCACGATCCT CAGCTCTTGA AGACAGTATT AAACAGTACG AGCAGGACCT GGCCAGGAGG 
CTGTACCAGT CCCGACAGCG GGCACCTGCT CCCGGGGCCG GGCCAGCTCC GATGCCTAGT 
CCCAAGCAGA GTCACGCCTC GGCACCCCCT GGGTCAGGAA GCCATTCCAG GATGAAATCG 
CTTGAACAGG ATGCTCTGAA AGCCCAGATG GTCATTGCCA AGTCCAAGGA GGGGAAGAAG 
CGCAGCCCGC TGGAGCAGCT GGCAGAGTCC CCCTCGCCAG TTCCTACACC GTCACCGACA 
CCACTGGAAG ATTGCAGTCC CAGGAACGTC ACCTCCCCAG GAAGGCTGTC CATGTCTGAA 
AAGAAGTTTG ACTACCGGGA ATTTGCTGCT ATTCCTTCCT CCAAACCCGT TTACGAAATT 
CAGTCGCCTG ATGCTGCTGA TGACCTCAGA TACATCGAGG ACAGGAATAA CTCAGTTCCC 
CAGGAGCAGG ATGGGCAGCC CGAGGAAGAG GACACATTAG GAACTCACGA CTCATCCACA 
CCCACTCCAT CAGCACTTGA AGAAATGGCC CTGTACAGCA GCAAACGCAA GCTCCGACAC 
AGCCGGCGCA GCCTGGAGGC TGCCGTCCCT ACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014801214.1
CDS	6..5501
Translation	MPPLWRCNRHVEALDRRHCSLQAVPEEIYRYSRSLEELLLDANQLRELPKPFFRLLNLRKLGLSDNEIQRLPPEVANFMQLVELDISRNDIPEIPESIKFCKSLEIADFSGNPLSRLPEGFTQLRSLGHLALNDVSLQSLPSDIGNLANLVTLELRENLLKTLPTSLSFLVKLEQLDLGGNDLEVLPDTLGALPNLRELWLDRNQLSALPPELGNLRRLVCLDVSENKLEQLPNEVSGLVALTDLLLSQNLLECIPDGIGQLKQLSILKVDQNRLTEVTESIGDCENLSELILTENMLTALPKSLGKLAKLTNLNVDRNRLTSLPAEIGGCANLNVLSLRDNRLTLLPSELANTTELHVLDVAGNRLQNLPFALTNLNLKALWLAENQSQPMLKFQTEDDEKTGEKVLTCYLLPQQPSPSLENLLQNSVDESWTDTNLNRVSVIQFLDEPKADDEEESGAERRGLQRRATPHPSELKVMKKVIEVRRSEAHAAKPNADLNSPHSEEKRLSATSNRSEDSHLSNSTASADCRAEEQQEEAERSRPNGQVPEMQELEKEAKSRVDEEHKEAAGHEEEDVEVEYNEPTVHFAEDTLIRSEDEDEDEERPFPVEKQRLIRKDTPHYKKHFKITKLPKPEAVVALLQGLNSEGVPKEEEELGGCNNNTEPADQEEVWDEEDRKNGALSAQPSVKGVSFDQANNLLIEPARIEEEELTLTIVRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVRVGDKLLEVNGVSLHSAEHHVAVEALRGSGSSVSMTVLRERMVEPENAITVTPLRPEDDYSPRERRGGLRFPERPEGTPPTERYSTCLMRNEKGLGFSIAGGKGSTPYRAGDMGIFISRIAEGGAAHRDGILHVGDRVISINGVDMTEARHDQAVALLTASSPTIVLLVEREGAEQPGEGDWPGGPRARMHSPPPPPSHGESPPEETPAPQRNHLSKGLEDQYPIEEIHLVKAGGPLGLSIVGGSDHSSHPFGIHEPGVFISKVIPRGLASRSGLRVGDRILEVNSIDLRHATHQEAVNALLSNTQELTMLVRRDPPPPGMQEICIEKAPGEKLGISIRGGAKGHAGNPFDPTDEGIFISKVSSSGAAARDGRLKVGMRILEVNHQSLLGMTHTEAVQMLRGVGDALLVLVCDGFDPKAAAAIEMSPGIIANPFAAGIGRKNSLESISSIDRDLSPEELEILQKEMEMVREATQWEREEMEKVNVTSEPLRLDYRTLAALPSSGLQRVNRTAPAGESGMGNSRRETPYSPGNQQRPATPRPAHLPGRETRFASINFITSQDDSKSTKPGVIQPLSRVRQNAAPHNSEEGDSPNPFQQPEPCFSVQNSPKPPSPPSPDEVPINVKQAYKTFAAVPLSHPLLGTQELPGQSGTENPRSAPEVAAHSPGGLHSPEQKSFRERQKYFEIEVKQQQMDKPPKRVSLVGEDDLKKMKEEEARKLQQKRTLLLEEEAEEEEMAKQAPEVSVQSSVIIEGVEYKIERLNGRSSQSPATRPSEVNENSSSQRNSLEEGIKAEQRTNSMSGLSPLYLGAGDAVAPVRTAKAERRHQERLRMQSPELLSGQEKELSPAERRALEAEKRAMWRAARSSALEDSIKQYEQDLARRLYQSRQRAPAPGAGPAPMPSPKQSHASAPPGSGSHSRMKSLEQDALKAQMVIAKSKEGKKRSPLEQLAESPSPVPTPSPTPLEDCSPRNVTSPGRLSMSEKKFDYREFAAIPSSKPVYEIQSPDAADDLRYIEDRNNSVPQEQDGQPEEEDTLGTHDSSTPTPSALEEMALYSSKRKLRHSRRSLEAAVPT

Target ORF information:

RefSeq Version	XM_014945728.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax scribbled planar cell polarity protein (SCRIB), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014945728.1

ORF Insert Sequence:

ATGCCGCCGC TCTGGCGCTG CAACCGGCAC GTGGAGGCGC TGGACCGGCG GCACTGCTCG 
CTGCAGGCCG TGCCCGAGGA GATCTACCGC TACAGCCGCT CGCTGGAGGA GCTGCTGCTG 
GACGCCAACC AGCTGCGGGA GCTGCCCAAG CCTTTCTTCA GGCTTCTGAA CCTGCGCAAG 
CTGGGCCTGA GTGACAATGA GATCCAAAGA CTTCCCCCTG AGGTGGCCAA CTTCATGCAG 
CTGGTGGAAC TGGACATCTC CCGTAACGAC ATTCCAGAAA TTCCTGAAAG CATCAAATTC 
TGCAAATCCT TGGAAATCGC AGACTTCAGT GGGAATCCTC TCTCCAGGCT TCCCGAGGGC 
TTCACGCAGC TTCGGAGCCT GGGCCATTTG GCCCTGAATG ATGTGTCCCT GCAGAGCCTC 
CCCAGTGACA TTGGGAATTT GGCAAACCTG GTGACTCTGG AGCTACGCGA GAACCTGCTA 
AAGACTCTCC CCACCTCGCT GTCCTTCCTT GTCAAGTTAG AGCAGCTGGA TCTCGGTGGC 
AATGACCTGG AGGTTCTGCC AGACACTCTG GGAGCGCTCC CCAACCTGCG TGAGCTCTGG 
TTAGACCGGA ACCAGCTCTC AGCCCTGCCT CCCGAGCTGG GCAACCTCCG CCGCCTGGTC 
TGCCTGGATG TGTCGGAGAA CAAGCTGGAG CAGTTGCCCA ACGAGGTCAG CGGCCTGGTG 
GCACTGACCG ACTTGCTGCT GTCGCAGAAC CTGTTGGAGT GCATTCCTGA TGGGATTGGT 
CAGCTGAAGC AGCTCTCCAT CCTCAAGGTG GACCAGAATC GACTGACAGA GGTGACAGAG 
TCCATTGGGG ACTGTGAAAA TCTGTCCGAG CTCATCCTGA CAGAGAACAT GCTGACGGCC 
TTGCCGAAAT CCCTTGGGAA GCTGGCCAAG CTGACCAACC TGAACGTGGA TCGGAACCGG 
CTGACATCCC TCCCGGCCGA GATCGGTGGC TGTGCCAACC TCAACGTGCT GTCCTTGCGG 
GACAATCGCC TGACCCTCCT GCCATCGGAG CTGGCCAACA CCACCGAGCT TCATGTTCTG 
GATGTGGCTG GGAACAGGCT GCAGAACTTG CCGTTTGCTC TGACAAACCT CAACCTGAAA 
GCCCTGTGGC TGGCGGAGAA TCAGTCCCAG CCCATGCTGA AATTCCAAAC AGAGGATGAT 
GAGAAGACAG GAGAGAAAGT TCTCACTTGT TACCTGCTTC CTCAGCAGCC CTCTCCCAGC 
CTGGAGAACC TTCTACAGAA CAGCGTGGAC GAGAGCTGGA CGGACACCAA TTTAAACAGA 
GTCAGTGTGA TTCAGTTCCT GGATGAACCC AAAGCAGATG ATGAAGAAGA GTCTGGAGCT 
GAGAGAAGGG GCTTGCAGCG CAGAGCAACC CCCCATCCCA GCGAGCTGAA GGTGATGAAG 
AAGGTGATTG AAGTTCGGCG CAGTGAGGCC CACGCTGCAA AGCCCAATGC AGACCTGAAT 
TCTCCTCACT CAGAAGAGAA GAGGCTGAGC GCCACGTCCA ACCGCAGCGA GGACTCCCAC 
CTTTCCAACA GCACTGCCTC TGCTGACTGT AGGGCGGAGG AGCAGCAGGA GGAGGCAGAG 
AGGAGTCGGC CAAACGGGCA AGTGCCTGAG ATGCAGGAGC TGGAGAAGGA GGCAAAGTCC 
AGAGTGGACG AGGAGCACAA GGAAGCTGCT GGCCATGAAG AGGAAGATGT GGAAGTGGAA 
TACAATGAGC CCACTGTACA CTTTGCTGAA GACACGCTAA TACGGAGTGA GGATGAAGAT 
GAAGATGAAG AGCGACCGTT CCCAGTGGAG AAGCAGCGGC TCATCCGCAA GGACACCCCT 
CACTATAAGA AACACTTTAA GATAACTAAA TTGCCCAAGC CAGAGGCGGT GGTGGCCCTG 
CTGCAGGGGC TGAACAGCGA GGGGGTCCCC AAGGAGGAAG AGGAGTTGGG AGGCTGCAAC 
AACAACACAG AGCCTGCAGA CCAGGAGGAG GTGTGGGATG AGGAGGACCG GAAGAATGGA 
GCTTTGTCTG CCCAGCCATC GGTGAAGGGG GTGTCGTTTG ATCAAGCCAA TAATCTGCTG 
ATTGAACCTG CTCGCATTGA GGAGGAAGAG TTGACTCTGA CTATCGTACG GCAGACGGGG 
GGGCTGGGCA TCAGCATCGC AGGGGGCAAG GGCTCCACCC CCTATAAGGG CGATGATGAG 
GGCATCTTTA TTTCTCGGGT GTCAGAAGAA GGTCCTGCGG CTCGTGCAGG GGTTCGTGTT 
GGGGACAAGC TCCTGGAGGT GAACGGTGTG TCCCTGCACT CCGCTGAGCA CCACGTGGCA 
GTGGAGGCTC TGCGTGGATC GGGAAGTTCC GTCTCCATGA CTGTTCTGCG GGAGCGGATG 
GTGGAGCCGG AGAATGCCAT CACTGTCACC CCGCTGCGCC CCGAGGATGA CTACAGCCCC 
CGTGAGAGGC GAGGTGGCCT GCGCTTCCCG GAGCGACCCG AGGGGACACC TCCCACCGAG 
AGATACTCCA CGTGCCTCAT GCGCAACGAG AAGGGACTGG GCTTCAGTAT CGCTGGTGGC 
AAAGGCTCCA CTCCTTACCG GGCTGGTGAC ATGGGGATCT TTATCTCCCG GATCGCAGAG 
GGTGGTGCAG CCCATCGGGA CGGGATCCTG CACGTAGGAG ATCGGGTCAT CTCGATCAAT 
GGGGTAGACA TGACAGAAGC CAGGCATGAT CAGGCAGTAG CGCTGCTTAC CGCGTCCTCC 
CCCACCATCG TGCTGCTGGT AGAGAGAGAG GGTGCGGAGC AGCCCGGCGA GGGAGACTGG 
CCCGGGGGGC CACGGGCACG CATGCACTCC CCACCGCCAC CCCCCAGCCA TGGGGAGAGC 
CCGCCCGAGG AGACGCCCGC ACCACAGAGG AACCATCTCT CCAAAGGCCT GGAGGATCAG 
TACCCCATTG AGGAAATTCA CCTGGTGAAA GCAGGTGGTC CCCTGGGGCT CAGCATCGTA 
GGGGGCAGTG ACCACTCAAG CCATCCGTTT GGGATTCATG AACCAGGTGT CTTCATTTCG 
AAGGTCATTC CCCGTGGGCT GGCATCCCGC AGTGGGCTGC GAGTAGGGGA CAGGATCCTG 
GAAGTCAATA GCATTGACCT GCGCCACGCT ACCCACCAGG AAGCGGTGAA CGCCCTGCTC 
TCCAACACCC AGGAGCTGAC CATGTTAGTG AGGCGAGACC CACCACCACC TGGTATGCAG 
GAAATATGCA TTGAGAAGGC TCCAGGAGAA AAGCTGGGCA TCAGCATCCG GGGTGGAGCG 
AAAGGCCATG CTGGAAATCC GTTTGATCCC ACTGACGAGG GCATCTTCAT CTCTAAGGTG 
AGCTCCTCGG GGGCGGCAGC CCGGGACGGC CGCCTGAAGG TGGGGATGCG GATCCTAGAG 
GTGAACCACC AGAGCCTGCT GGGGATGACG CACACGGAAG CGGTGCAGAT GCTGCGCGGG 
GTGGGCGACG CGCTCCTGGT GCTGGTGTGT GACGGCTTCG ACCCCAAGGC TGCAGCTGCC 
ATCGAGATGT CCCCGGGAAT TATTGCAAAC CCTTTCGCTG CTGGTATCGG ACGCAAGAAT 
AGCCTGGAAA GTATCTCTTC CATCGACCGG GATTTAAGCC CTGAGGAACT GGAGATCCTG 
CAGAAGGAGA TGGAAATGGT GAGAGAAGCA ACTCAGTGGG AGAGAGAAGA AATGGAGAAA 
GTGAACGTCA CCAGCGAGCC CTTGAGACTG GACTATCGGA CCCTGGCTGC GCTGCCGAGC 
AGTGGTCTCC AGAGAGTCAA CCGCACCGCT CCTGCAGGCG AGTCTGGGAT GGGCAATTCC 
AGGCGTGAAA CCCCCTATTC TCCTGGTAAC CAGCAGCGGC CTGCAACCCC TCGGCCCGCT 
CACCTGCCGG GCAGGGAGAC CCGCTTTGCT TCCATTAACT TCATTACATC GCAGGATGAC 
AGTAAATCGA CCAAGCCAGG CGTCATTCAG CCTCTTTCCC GAGTGCGACA AAATGCTGCC 
CCGCACAATT CGGAGGAGGG GGACAGCCCC AATCCCTTCC AGCAGCCCGA GCCGTGCTTC 
AGTGTCCAGA ACTCTCCAAA GCCACCCTCA CCACCCTCTC CTGATGAGGT CCCCATCAAT 
GTCAAGCAAG CGTACAAGAC CTTCGCTGCA GTGCCCTTGT CACACCCCCT GCTTGGGACA 
CAGGAATTGC CTGGTCAGAG CGGCACAGAG AATCCCAGAA GTGCCCCGGA GGTGGCTGCG 
CACAGCCCTG GGGGACTGCA CAGCCCTGAG CAGAAGTCCT TCCGTGAGAG GCAGAAGTAT 
TTCGAGATTG AGGTGAAGCA GCAGCAGATG GACAAGCCCC CCAAGAGAGT CTCCTTGGTG 
GGAGAGGACG ACCTGAAGAA GATGAAAGAG GAGGAAGCTC GAAAACTGCA GCAGAAACGT 
ACCCTTCTGC TGGAGGAAGA GGCAGAAGAG GAGGAGATGG CGAAGCAGGC GCCTGAGGTG 
AGCGTGCAGT CCAGTGTCAT CATTGAAGGA GTCGAGTACA AGATTGAGAG ACTCAACGGG 
AGGAGCAGCC AGTCTCCGGC CACTCGGCCC TCCGAGGTCA ACGAGAACAG CAGCTCGCAG 
AGGAATTCAC TGGAAGAGGG GATCAAGGCT GAGCAGAGAA CCAACTCCAT GTCTGGGCTG 
AGTCCCTTGT ACCTTGGAGC AGGGGACGCA GTGGCACCCG TGCGGACGGC CAAAGCTGAA 
CGGCGACACC AGGAGCGGTT GCGAATGCAG AGTCCTGAGC TGCTGAGTGG CCAAGAGAAG 
GAGCTTTCTC CTGCAGAACG TCGTGCTCTG GAGGCGGAGA AACGAGCAAT GTGGAGGGCA 
GCACGATCCT CAGCTCTTGA AGACAGTATT AAACAGTACG AGCAGGACCT GGCCAGGAGG 
CTGTACCAGT CCCGACAGCG GGCACCTGCT CCCGGGGCCG GGCCAGCTCC GATGCCTAGT 
CCCAAGCAGA GTCACGCCTC GGCACCCCCT GGGTCAGGAA GCCATTCCAG GATGAAATCG 
CTTGAACAGG ATGCTCTGAA AGCCCAGATG GTCATTGCCA AGTCCAAGGA GGGGAAGAAG 
CGCAGCCCGC TGGAGCAGCT GGCAGAGTCC CCCTCGCCAG TTCCTACACC GTCACCGACA 
CCACTGGAAG ATTGCAGTCC CAGGAACGTC ACCTCCCCAG GAAGGCTGTC CATGTCTGAA 
AAGAAGTTTG ACTACCGGGA ATTTGCTGCT ATTCCTTCCT CCAAACCCGT TTACGAAATT 
CAGTCGCCTG ATGCTGCTGA TGACCTCAGA TACATCGAGG ACAGGAATAA CTCAGTTCCC 
CAGGAGCAGG ATGGGCAGCC CGAGGAAGAG GACACATTAG GAACTCACGA CTCATCCACA 
CCCACTCCAT CAGCACTTGA AGAAATGGCC CTGTACAGCA GCAAACGCAA GCTCCGACAC 
AGCCGGCGCA GCCTGGAGGC TGCCGTCCCT ACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCRIB ( XM_014945729.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014801215.1 Calidris pugnax scribbled planar cell polarity protein (SCRIB), transcript variant X15, mRNA.

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CloneID	OCa67761
Clone ID Related Accession (Same CDS sequence)	XM_014945729.1
Accession Version	XM_014945729.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5439bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	protein scribble homolog isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090871.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCCGC TCTGGCGCTG CAACCGGCAC GTGGAGGCGC TGGACCGGCG GCACTGCTCG 
CTGCAGGCCG TGCCCGAGGA GATCTACCGC TACAGCCGCT CGCTGGAGGA GCTGCTGCTG 
GACGCCAACC AGCTGCGGGA GCTGCCCAAG CCTTTCTTCA GGCTTCTGAA CCTGCGCAAG 
CTGGGCCTGA GTGACAATGA GATCCAAAGA CTTCCCCCTG AGGTGGCCAA CTTCATGCAG 
CTGGTGGAAC TGGACATCTC CCGTAACGAC ATTCCAGAAA TTCCTGAAAG CATCAAATTC 
TGCAAATCCT TGGAAATCGC AGACTTCAGT GGGAATCCTC TCTCCAGGCT TCCCGAGGGC 
TTCACGCAGC TTCGGAGCCT GGGCCATTTG GCCCTGAATG ATGTGTCCCT GCAGAGCCTC 
CCCAGTGACA TTGGGAATTT GGCAAACCTG GTGACTCTGG AGCTACGCGA GAACCTGCTA 
AAGACTCTCC CCACCTCGCT GTCCTTCCTT GTCAAGTTAG AGCAGCTGGA TCTCGGTGGC 
AATGACCTGG AGGTTCTGCC AGACACTCTG GGAGCGCTCC CCAACCTGCG TGAGCTCTGG 
TTAGACCGGA ACCAGCTCTC AGCCCTGCCT CCCGAGCTGG GCAACCTCCG CCGCCTGGTC 
TGCCTGGATG TGTCGGAGAA CAAGCTGGAG CAGTTGCCCA ACGAGGTCAG CGGCCTGGTG 
GCACTGACCG ACTTGCTGCT GTCGCAGAAC CTGTTGGAGT GCATTCCTGA TGGGATTGGT 
CAGCTGAAGC AGCTCTCCAT CCTCAAGGTG GACCAGAATC GACTGACAGA GGTGACAGAG 
TCCATTGGGG ACTGTGAAAA TCTGTCCGAG CTCATCCTGA CAGAGAACAT GCTGACGGCC 
TTGCCGAAAT CCCTTGGGAA GCTGGCCAAG CTGACCAACC TGAACGTGGA TCGGAACCGG 
CTGACATCCC TCCCGGCCGA GATCGGTGGC TGTGCCAACC TCAACGTGCT GTCCTTGCGG 
GACAATCGCC TGACCCTCCT GCCATCGGAG CTGGCCAACA CCACCGAGCT TCATGTTCTG 
GATGTGGCTG GGAACAGGCT GCAGAACTTG CCGTTTGCTC TGACAAACCT CAACCTGAAA 
GCCCTGTGGC TGGCGGAGAA TCAGTCCCAG CCCATGCTGA AATTCCAAAC AGAGGATGAT 
GAGAAGACAG GAGAGAAAGT TCTCACTTGT TACCTGCTTC CTCAGCAGCC CTCTCCCAGC 
CTGGAGAACC TTCTACAGAA CAGCGTGGAC GAGAGCTGGA CGGACACCAA TTTAAACAGA 
GTCAGTGTGA TTCAGTTCCT GGATGAACCC AAAGCAGATG ATGAAGAAGA GTCTGGAGCT 
GAGAGAAGGG GCTTGCAGCG CAGAGCAACC CCCCATCCCA GCGAGCTGAA GGTGATGAAG 
AAGGTGATTG AAGTTCGGCG CAGTGAGGCC CACGCTGCAA AGCCCAATGC AGACCTGAAT 
TCTCCTCACT CAGAAGAGAA GAGGCTGAGC GCCACGTCCA ACCGCAGCGA GGACTCCCAC 
CTTTCCAACA GCACTGCCTC TGCTGACTGT AGGGCGGAGG AGCAGCAGGA GGAGGCAGAG 
AGGAGTCGGC CAAACGGGCA AGTGCCTGAG ATGCAGGAGC TGGAGAAGGA GGCAAAGTCC 
AGAGTGGACG AGGAGCACAA GGAAGCTGCT GGCCATGAAG AGGAAGATGT GGAAGTGGAA 
TACAATGAGC CCACTGTACA CTTTGCTGAA GACACGCTAA TACGGAGTGA GGATGAAGAT 
GAAGATGAAG AGCGACCGTT CCCAGTGGAG AAGCAGCGGC TCATCCGCAA GGACACCCCT 
CACTATAAGA AACACTTTAA GATAACTAAA TTGCCCAAGC CAGAGGCGGT GGTGGCCCTG 
CTGCAGGGGC TGAACAGCGA GGGGGTCCCC AAGGAGGAAG AGGAGTTGGG AGGCTGCAAC 
AACAACACAG AGCCTGCAGA CCAGGAGGAG GTGTGGGATG AGGAGGACCG GAAGAATGGA 
GCTTTGTCTG CCCAGCCATC GGTGAAGGGG GTGTCGTTTG ATCAAGCCAA TAATCTGCTG 
ATTGAACCTG CTCGCATTGA GGAGGAAGAG TTGACTCTGA CTATCGTACG GCAGACGGGG 
GGGCTGGGCA TCAGCATCGC AGGGGGCAAG GGCTCCACCC CCTATAAGGG CGATGATGAG 
GGCATCTTTA TTTCTCGGGT GTCAGAAGAA GGTCCTGCGG CTCGTGCAGG GGTTCGTGTT 
GGGGACAAGC TCCTGGAGGT GAACGGTGTG TCCCTGCACT CCGCTGAGCA CCACGTGGCA 
GTGGAGGCTC TGCGTGGATC GGGAAGTTCC GTCTCCATGA CTGTTCTGCG GGAGCGGATG 
GTGGAGCCGG AGAATGCCAT CACTGTCACC CCGCTGCGCC CCGAGGATGA CTACAGCCCC 
CGTGAGAGGC GAGGTGGCCT GCGCTTCCCG GAGCGACCCG AGGGGACACC TCCCACCGAG 
AGATACTCCA CGTGCCTCAT GCGCAACGAG AAGGGACTGG GCTTCAGTAT CGCTGGTGGC 
AAAGGCTCCA CTCCTTACCG GGCTGGTGAC ATGGGGATCT TTATCTCCCG GATCGCAGAG 
GGTGGTGCAG CCCATCGGGA CGGGATCCTG CACGTAGGAG ATCGGGTCAT CTCGATCAAT 
GGGGTAGACA TGACAGAAGC CAGGCATGAT CAGGCAGTAG CGCTGCTTAC CGCGTCCTCC 
CCCACCATCG TGCTGCTGGT AGAGAGAGAG GGTGCGGAGC AGCCCGGCGA GGGAGACTGG 
CCCGGGGGGC CACGGGCACG CATGCACTCC CCACCGCCAC CCCCCAGCCA TGGGGAGAGC 
CCGCCCGAGG AGACGCCCGC ACCACAGAGG AACCATCTCT CCAAAGGCCT GGAGGATCAG 
TACCCCATTG AGGAAATTCA CCTGGTGAAA GCAGGTGGTC CCCTGGGGCT CAGCATCGTA 
GGGGGCAGTG ACCACTCAAG CCATCCGTTT GGGATTCATG AACCAGGTGT CTTCATTTCG 
AAGGTCATTC CCCGTGGGCT GGCATCCCGC AGTGGGCTGC GAGTAGGGGA CAGGATCCTG 
GAAGTCAATA GCATTGACCT GCGCCACGCT ACCCACCAGG AAGCGGTGAA CGCCCTGCTC 
TCCAACACCC AGGAGCTGAC CATGTTAGTG AGGCGAGACC CACCACCACC TGGTATGCAG 
GAAATATGCA TTGAGAAGGC TCCAGGAGAA AAGCTGGGCA TCAGCATCCG GGGTGGAGCG 
AAAGGCCATG CTGGAAATCC GTTTGATCCC ACTGACGAGG GCATCTTCAT CTCTAAGGTG 
AGCTCCTCGG GGGCGGCAGC CCGGGACGGC CGCCTGAAGG TGGGGATGCG GATCCTAGAG 
GTGAACCACC AGAGCCTGCT GGGGATGACG CACACGGAAG CGGTGCAGAT GCTGCGCGGG 
GTGGGCGACG CGCTCCTGGT GCTGGTGTGT GACGGCTTCG ACCCCAAGGC TGCAGCTGCC 
ATCGAGATGT CCCCGGGAAT TATTGCAAAC CCTTTCGCTG CTGGTATCGG ACGCAAGAAT 
AGCCTGGAAA GTATCTCTTC CATCGACCGG GATTTAAGCC CTGAGGAACT GGAGATCCTG 
CAGAAGAACG TCACCAGCGA GCCCTTGAGA CTGGACTATC GGACCCTGGC TGCGCTGCCG 
AGCAGTGGTC TCCAGAGAGT CAACCGCACC GCTCCTGCAG GCGAGTCTGG GATGGGCAAT 
TCCAGGCGTG AAACCCCCTA TTCTCCTGGT AACCAGCAGC GGCCTGCAAC CCCTCGGCCC 
GCTCACCTGC CGGGCAGGGA GACCCGCTTT GCTTCCATTA ACTTCATTAC ATCGCAGGAT 
GACAGTAAAT CGACCAAGCC AGGCGTCATT CAGCCTCTTT CCCGAGTGCG ACAAAATGCT 
GCCCCGCACA ATTCGGAGGA GGGGGACAGC CCCAATCCCT TCCAGCAGCC CGAGCCGTGC 
TTCAGTGTCC AGAACTCTCC AAAGCCACCC TCACCACCCT CTCCTGATGA GGTCCCCATC 
AATGTCAAGC AAGCGTACAA GACCTTCGCT GCAGTGCCCT TGTCACACCC CCTGCTTGGG 
ACACAGGAAT TGCCTGGTCA GAGCGGCACA GAGAATCCCA GAAGTGCCCC GGAGGTGGCT 
GCGCACAGCC CTGGGGGACT GCACAGCCCT GAGCAGAAGT CCTTCCGTGA GAGGCAGAAG 
TATTTCGAGA TTGAGGTGAA GCAGCAGCAG ATGGACAAGC CCCCCAAGAG AGTCTCCTTG 
GTGGGAGAGG ACGACCTGAA GAAGATGAAA GAGGAGGAAG CTCGAAAACT GCAGCAGAAA 
CGTACCCTTC TGCTGGAGGA AGAGGCAGAA GAGGAGGAGA TGGCGAAGCA GGCGCCTGAG 
GTGAGCGTGC AGTCCAGTGT CATCATTGAA GGAGTCGAGT ACAAGATTGA GAGACTCAAC 
GGGAGGAGCA GCCAGTCTCC GGCCACTCGG CCCTCCGAGG TCAACGAGAA CAGCAGCTCG 
CAGAGGAATT CACTGGAAGA GGGGATCAAG GCTGAGCAGA GAACCAACTC CATGTCTGGG 
CTGAGTCCCT TGTACCTTGG AGCAGGGGAC GCAGTGGCAC CCGTGCGGAC GGCCAAAGCT 
GAACGGCGAC ACCAGGAGCG GTTGCGAATG CAGAGTCCTG AGCTGCTGAG TGGCCAAGAG 
AAGGAGCTTT CTCCTGCAGA ACGTCGTGCT CTGGAGGCGG AGAAACGAGC AATGTGGAGG 
GCAGCACGAT CCTCAGCTCT TGAAGACAGT ATTAAACAGT ACGAGCAGGA CCTGGCCAGG 
AGGCTGTACC AGTCCCGACA GCGGGCACCT GCTCCCGGGG CCGGGCCAGC TCCGATGCCT 
AGTCCCAAGC AGAGTCACGC CTCGGCACCC CCTGGGTCAG GAAGCCATTC CAGGATGAAA 
TCGCTTGAAC AGGATGCTCT GAAAGCCCAG ATGGTCATTG CCAAGTCCAA GGAGGGGAAG 
AAGCGCAGCC CGCTGGAGCA GCTGGCAGAG TCCCCCTCGC CAGTTCCTAC ACCGTCACCG 
ACACCACTGG AAGATTGCAG TCCCAGGAAC GTCACCTCCC CAGGAAGGCT GTCCATGTCT 
GAAAAGAAGT TTGACTACCG GGAATTTGCT GCTATTCCTT CCTCCAAACC CGTTTACGAA 
ATTCAGTCGC CTGATGCTGC TGATGACCTC AGATACATCG AGGACAGGAA TAACTCAGTT 
CCCCAGGAGC AGGATGGGCA GCCCGAGGAA GAGGACACAT TAGGAACTCA CGACTCATCC 
ACACCCACTC CATCAGCACT TGAAGAAATG GCCCTGTACA GCAGCAAACG CAAGCTCCGA 
CACAGCCGGC GCAGCCTGGA GGCTGCCGTC CCTACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014801215.1
CDS	6..5444
Translation	MPPLWRCNRHVEALDRRHCSLQAVPEEIYRYSRSLEELLLDANQLRELPKPFFRLLNLRKLGLSDNEIQRLPPEVANFMQLVELDISRNDIPEIPESIKFCKSLEIADFSGNPLSRLPEGFTQLRSLGHLALNDVSLQSLPSDIGNLANLVTLELRENLLKTLPTSLSFLVKLEQLDLGGNDLEVLPDTLGALPNLRELWLDRNQLSALPPELGNLRRLVCLDVSENKLEQLPNEVSGLVALTDLLLSQNLLECIPDGIGQLKQLSILKVDQNRLTEVTESIGDCENLSELILTENMLTALPKSLGKLAKLTNLNVDRNRLTSLPAEIGGCANLNVLSLRDNRLTLLPSELANTTELHVLDVAGNRLQNLPFALTNLNLKALWLAENQSQPMLKFQTEDDEKTGEKVLTCYLLPQQPSPSLENLLQNSVDESWTDTNLNRVSVIQFLDEPKADDEEESGAERRGLQRRATPHPSELKVMKKVIEVRRSEAHAAKPNADLNSPHSEEKRLSATSNRSEDSHLSNSTASADCRAEEQQEEAERSRPNGQVPEMQELEKEAKSRVDEEHKEAAGHEEEDVEVEYNEPTVHFAEDTLIRSEDEDEDEERPFPVEKQRLIRKDTPHYKKHFKITKLPKPEAVVALLQGLNSEGVPKEEEELGGCNNNTEPADQEEVWDEEDRKNGALSAQPSVKGVSFDQANNLLIEPARIEEEELTLTIVRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVRVGDKLLEVNGVSLHSAEHHVAVEALRGSGSSVSMTVLRERMVEPENAITVTPLRPEDDYSPRERRGGLRFPERPEGTPPTERYSTCLMRNEKGLGFSIAGGKGSTPYRAGDMGIFISRIAEGGAAHRDGILHVGDRVISINGVDMTEARHDQAVALLTASSPTIVLLVEREGAEQPGEGDWPGGPRARMHSPPPPPSHGESPPEETPAPQRNHLSKGLEDQYPIEEIHLVKAGGPLGLSIVGGSDHSSHPFGIHEPGVFISKVIPRGLASRSGLRVGDRILEVNSIDLRHATHQEAVNALLSNTQELTMLVRRDPPPPGMQEICIEKAPGEKLGISIRGGAKGHAGNPFDPTDEGIFISKVSSSGAAARDGRLKVGMRILEVNHQSLLGMTHTEAVQMLRGVGDALLVLVCDGFDPKAAAAIEMSPGIIANPFAAGIGRKNSLESISSIDRDLSPEELEILQKNVTSEPLRLDYRTLAALPSSGLQRVNRTAPAGESGMGNSRRETPYSPGNQQRPATPRPAHLPGRETRFASINFITSQDDSKSTKPGVIQPLSRVRQNAAPHNSEEGDSPNPFQQPEPCFSVQNSPKPPSPPSPDEVPINVKQAYKTFAAVPLSHPLLGTQELPGQSGTENPRSAPEVAAHSPGGLHSPEQKSFRERQKYFEIEVKQQQMDKPPKRVSLVGEDDLKKMKEEEARKLQQKRTLLLEEEAEEEEMAKQAPEVSVQSSVIIEGVEYKIERLNGRSSQSPATRPSEVNENSSSQRNSLEEGIKAEQRTNSMSGLSPLYLGAGDAVAPVRTAKAERRHQERLRMQSPELLSGQEKELSPAERRALEAEKRAMWRAARSSALEDSIKQYEQDLARRLYQSRQRAPAPGAGPAPMPSPKQSHASAPPGSGSHSRMKSLEQDALKAQMVIAKSKEGKKRSPLEQLAESPSPVPTPSPTPLEDCSPRNVTSPGRLSMSEKKFDYREFAAIPSSKPVYEIQSPDAADDLRYIEDRNNSVPQEQDGQPEEEDTLGTHDSSTPTPSALEEMALYSSKRKLRHSRRSLEAAVPT

Target ORF information:

RefSeq Version	XM_014945729.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax scribbled planar cell polarity protein (SCRIB), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014945729.1

ORF Insert Sequence:

ATGCCGCCGC TCTGGCGCTG CAACCGGCAC GTGGAGGCGC TGGACCGGCG GCACTGCTCG 
CTGCAGGCCG TGCCCGAGGA GATCTACCGC TACAGCCGCT CGCTGGAGGA GCTGCTGCTG 
GACGCCAACC AGCTGCGGGA GCTGCCCAAG CCTTTCTTCA GGCTTCTGAA CCTGCGCAAG 
CTGGGCCTGA GTGACAATGA GATCCAAAGA CTTCCCCCTG AGGTGGCCAA CTTCATGCAG 
CTGGTGGAAC TGGACATCTC CCGTAACGAC ATTCCAGAAA TTCCTGAAAG CATCAAATTC 
TGCAAATCCT TGGAAATCGC AGACTTCAGT GGGAATCCTC TCTCCAGGCT TCCCGAGGGC 
TTCACGCAGC TTCGGAGCCT GGGCCATTTG GCCCTGAATG ATGTGTCCCT GCAGAGCCTC 
CCCAGTGACA TTGGGAATTT GGCAAACCTG GTGACTCTGG AGCTACGCGA GAACCTGCTA 
AAGACTCTCC CCACCTCGCT GTCCTTCCTT GTCAAGTTAG AGCAGCTGGA TCTCGGTGGC 
AATGACCTGG AGGTTCTGCC AGACACTCTG GGAGCGCTCC CCAACCTGCG TGAGCTCTGG 
TTAGACCGGA ACCAGCTCTC AGCCCTGCCT CCCGAGCTGG GCAACCTCCG CCGCCTGGTC 
TGCCTGGATG TGTCGGAGAA CAAGCTGGAG CAGTTGCCCA ACGAGGTCAG CGGCCTGGTG 
GCACTGACCG ACTTGCTGCT GTCGCAGAAC CTGTTGGAGT GCATTCCTGA TGGGATTGGT 
CAGCTGAAGC AGCTCTCCAT CCTCAAGGTG GACCAGAATC GACTGACAGA GGTGACAGAG 
TCCATTGGGG ACTGTGAAAA TCTGTCCGAG CTCATCCTGA CAGAGAACAT GCTGACGGCC 
TTGCCGAAAT CCCTTGGGAA GCTGGCCAAG CTGACCAACC TGAACGTGGA TCGGAACCGG 
CTGACATCCC TCCCGGCCGA GATCGGTGGC TGTGCCAACC TCAACGTGCT GTCCTTGCGG 
GACAATCGCC TGACCCTCCT GCCATCGGAG CTGGCCAACA CCACCGAGCT TCATGTTCTG 
GATGTGGCTG GGAACAGGCT GCAGAACTTG CCGTTTGCTC TGACAAACCT CAACCTGAAA 
GCCCTGTGGC TGGCGGAGAA TCAGTCCCAG CCCATGCTGA AATTCCAAAC AGAGGATGAT 
GAGAAGACAG GAGAGAAAGT TCTCACTTGT TACCTGCTTC CTCAGCAGCC CTCTCCCAGC 
CTGGAGAACC TTCTACAGAA CAGCGTGGAC GAGAGCTGGA CGGACACCAA TTTAAACAGA 
GTCAGTGTGA TTCAGTTCCT GGATGAACCC AAAGCAGATG ATGAAGAAGA GTCTGGAGCT 
GAGAGAAGGG GCTTGCAGCG CAGAGCAACC CCCCATCCCA GCGAGCTGAA GGTGATGAAG 
AAGGTGATTG AAGTTCGGCG CAGTGAGGCC CACGCTGCAA AGCCCAATGC AGACCTGAAT 
TCTCCTCACT CAGAAGAGAA GAGGCTGAGC GCCACGTCCA ACCGCAGCGA GGACTCCCAC 
CTTTCCAACA GCACTGCCTC TGCTGACTGT AGGGCGGAGG AGCAGCAGGA GGAGGCAGAG 
AGGAGTCGGC CAAACGGGCA AGTGCCTGAG ATGCAGGAGC TGGAGAAGGA GGCAAAGTCC 
AGAGTGGACG AGGAGCACAA GGAAGCTGCT GGCCATGAAG AGGAAGATGT GGAAGTGGAA 
TACAATGAGC CCACTGTACA CTTTGCTGAA GACACGCTAA TACGGAGTGA GGATGAAGAT 
GAAGATGAAG AGCGACCGTT CCCAGTGGAG AAGCAGCGGC TCATCCGCAA GGACACCCCT 
CACTATAAGA AACACTTTAA GATAACTAAA TTGCCCAAGC CAGAGGCGGT GGTGGCCCTG 
CTGCAGGGGC TGAACAGCGA GGGGGTCCCC AAGGAGGAAG AGGAGTTGGG AGGCTGCAAC 
AACAACACAG AGCCTGCAGA CCAGGAGGAG GTGTGGGATG AGGAGGACCG GAAGAATGGA 
GCTTTGTCTG CCCAGCCATC GGTGAAGGGG GTGTCGTTTG ATCAAGCCAA TAATCTGCTG 
ATTGAACCTG CTCGCATTGA GGAGGAAGAG TTGACTCTGA CTATCGTACG GCAGACGGGG 
GGGCTGGGCA TCAGCATCGC AGGGGGCAAG GGCTCCACCC CCTATAAGGG CGATGATGAG 
GGCATCTTTA TTTCTCGGGT GTCAGAAGAA GGTCCTGCGG CTCGTGCAGG GGTTCGTGTT 
GGGGACAAGC TCCTGGAGGT GAACGGTGTG TCCCTGCACT CCGCTGAGCA CCACGTGGCA 
GTGGAGGCTC TGCGTGGATC GGGAAGTTCC GTCTCCATGA CTGTTCTGCG GGAGCGGATG 
GTGGAGCCGG AGAATGCCAT CACTGTCACC CCGCTGCGCC CCGAGGATGA CTACAGCCCC 
CGTGAGAGGC GAGGTGGCCT GCGCTTCCCG GAGCGACCCG AGGGGACACC TCCCACCGAG 
AGATACTCCA CGTGCCTCAT GCGCAACGAG AAGGGACTGG GCTTCAGTAT CGCTGGTGGC 
AAAGGCTCCA CTCCTTACCG GGCTGGTGAC ATGGGGATCT TTATCTCCCG GATCGCAGAG 
GGTGGTGCAG CCCATCGGGA CGGGATCCTG CACGTAGGAG ATCGGGTCAT CTCGATCAAT 
GGGGTAGACA TGACAGAAGC CAGGCATGAT CAGGCAGTAG CGCTGCTTAC CGCGTCCTCC 
CCCACCATCG TGCTGCTGGT AGAGAGAGAG GGTGCGGAGC AGCCCGGCGA GGGAGACTGG 
CCCGGGGGGC CACGGGCACG CATGCACTCC CCACCGCCAC CCCCCAGCCA TGGGGAGAGC 
CCGCCCGAGG AGACGCCCGC ACCACAGAGG AACCATCTCT CCAAAGGCCT GGAGGATCAG 
TACCCCATTG AGGAAATTCA CCTGGTGAAA GCAGGTGGTC CCCTGGGGCT CAGCATCGTA 
GGGGGCAGTG ACCACTCAAG CCATCCGTTT GGGATTCATG AACCAGGTGT CTTCATTTCG 
AAGGTCATTC CCCGTGGGCT GGCATCCCGC AGTGGGCTGC GAGTAGGGGA CAGGATCCTG 
GAAGTCAATA GCATTGACCT GCGCCACGCT ACCCACCAGG AAGCGGTGAA CGCCCTGCTC 
TCCAACACCC AGGAGCTGAC CATGTTAGTG AGGCGAGACC CACCACCACC TGGTATGCAG 
GAAATATGCA TTGAGAAGGC TCCAGGAGAA AAGCTGGGCA TCAGCATCCG GGGTGGAGCG 
AAAGGCCATG CTGGAAATCC GTTTGATCCC ACTGACGAGG GCATCTTCAT CTCTAAGGTG 
AGCTCCTCGG GGGCGGCAGC CCGGGACGGC CGCCTGAAGG TGGGGATGCG GATCCTAGAG 
GTGAACCACC AGAGCCTGCT GGGGATGACG CACACGGAAG CGGTGCAGAT GCTGCGCGGG 
GTGGGCGACG CGCTCCTGGT GCTGGTGTGT GACGGCTTCG ACCCCAAGGC TGCAGCTGCC 
ATCGAGATGT CCCCGGGAAT TATTGCAAAC CCTTTCGCTG CTGGTATCGG ACGCAAGAAT 
AGCCTGGAAA GTATCTCTTC CATCGACCGG GATTTAAGCC CTGAGGAACT GGAGATCCTG 
CAGAAGAACG TCACCAGCGA GCCCTTGAGA CTGGACTATC GGACCCTGGC TGCGCTGCCG 
AGCAGTGGTC TCCAGAGAGT CAACCGCACC GCTCCTGCAG GCGAGTCTGG GATGGGCAAT 
TCCAGGCGTG AAACCCCCTA TTCTCCTGGT AACCAGCAGC GGCCTGCAAC CCCTCGGCCC 
GCTCACCTGC CGGGCAGGGA GACCCGCTTT GCTTCCATTA ACTTCATTAC ATCGCAGGAT 
GACAGTAAAT CGACCAAGCC AGGCGTCATT CAGCCTCTTT CCCGAGTGCG ACAAAATGCT 
GCCCCGCACA ATTCGGAGGA GGGGGACAGC CCCAATCCCT TCCAGCAGCC CGAGCCGTGC 
TTCAGTGTCC AGAACTCTCC AAAGCCACCC TCACCACCCT CTCCTGATGA GGTCCCCATC 
AATGTCAAGC AAGCGTACAA GACCTTCGCT GCAGTGCCCT TGTCACACCC CCTGCTTGGG 
ACACAGGAAT TGCCTGGTCA GAGCGGCACA GAGAATCCCA GAAGTGCCCC GGAGGTGGCT 
GCGCACAGCC CTGGGGGACT GCACAGCCCT GAGCAGAAGT CCTTCCGTGA GAGGCAGAAG 
TATTTCGAGA TTGAGGTGAA GCAGCAGCAG ATGGACAAGC CCCCCAAGAG AGTCTCCTTG 
GTGGGAGAGG ACGACCTGAA GAAGATGAAA GAGGAGGAAG CTCGAAAACT GCAGCAGAAA 
CGTACCCTTC TGCTGGAGGA AGAGGCAGAA GAGGAGGAGA TGGCGAAGCA GGCGCCTGAG 
GTGAGCGTGC AGTCCAGTGT CATCATTGAA GGAGTCGAGT ACAAGATTGA GAGACTCAAC 
GGGAGGAGCA GCCAGTCTCC GGCCACTCGG CCCTCCGAGG TCAACGAGAA CAGCAGCTCG 
CAGAGGAATT CACTGGAAGA GGGGATCAAG GCTGAGCAGA GAACCAACTC CATGTCTGGG 
CTGAGTCCCT TGTACCTTGG AGCAGGGGAC GCAGTGGCAC CCGTGCGGAC GGCCAAAGCT 
GAACGGCGAC ACCAGGAGCG GTTGCGAATG CAGAGTCCTG AGCTGCTGAG TGGCCAAGAG 
AAGGAGCTTT CTCCTGCAGA ACGTCGTGCT CTGGAGGCGG AGAAACGAGC AATGTGGAGG 
GCAGCACGAT CCTCAGCTCT TGAAGACAGT ATTAAACAGT ACGAGCAGGA CCTGGCCAGG 
AGGCTGTACC AGTCCCGACA GCGGGCACCT GCTCCCGGGG CCGGGCCAGC TCCGATGCCT 
AGTCCCAAGC AGAGTCACGC CTCGGCACCC CCTGGGTCAG GAAGCCATTC CAGGATGAAA 
TCGCTTGAAC AGGATGCTCT GAAAGCCCAG ATGGTCATTG CCAAGTCCAA GGAGGGGAAG 
AAGCGCAGCC CGCTGGAGCA GCTGGCAGAG TCCCCCTCGC CAGTTCCTAC ACCGTCACCG 
ACACCACTGG AAGATTGCAG TCCCAGGAAC GTCACCTCCC CAGGAAGGCT GTCCATGTCT 
GAAAAGAAGT TTGACTACCG GGAATTTGCT GCTATTCCTT CCTCCAAACC CGTTTACGAA 
ATTCAGTCGC CTGATGCTGC TGATGACCTC AGATACATCG AGGACAGGAA TAACTCAGTT 
CCCCAGGAGC AGGATGGGCA GCCCGAGGAA GAGGACACAT TAGGAACTCA CGACTCATCC 
ACACCCACTC CATCAGCACT TGAAGAAATG GCCCTGTACA GCAGCAAACG CAAGCTCCGA 
CACAGCCGGC GCAGCCTGGA GGCTGCCGTC CCTACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCRIB ( XM_014945730.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014801216.1 Calidris pugnax scribbled planar cell polarity protein (SCRIB), transcript variant X16, mRNA.

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CloneID	OCa67762
Clone ID Related Accession (Same CDS sequence)	XM_014945730.1
Accession Version	XM_014945730.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5376bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	protein scribble homolog isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090871.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCCGC TCTGGCGCTG CAACCGGCAC GTGGAGGCGC TGGACCGGCG GCACTGCTCG 
CTGCAGGCCG TGCCCGAGGA GATCTACCGC TACAGCCGCT CGCTGGAGGA GCTGCTGCTG 
GACGCCAACC AGCTGCGGGA GCTGCCCAAG CCTTTCTTCA GGCTTCTGAA CCTGCGCAAG 
CTGGGCCTGA GTGACAATGA GATCCAAAGA CTTCCCCCTG AGGTGGCCAA CTTCATGCAG 
CTGGTGGAAC TGGACATCTC CCGTAACGAC ATTCCAGAAA TTCCTGAAAG CATCAAATTC 
TGCAAATCCT TGGAAATCGC AGACTTCAGT GGGAATCCTC TCTCCAGGCT TCCCGAGGGC 
TTCACGCAGC TTCGGAGCCT GGGCCATTTG GCCCTGAATG ATGTGTCCCT GCAGAGCCTC 
CCCAGTGACA TTGGGAATTT GGCAAACCTG GTGACTCTGG AGCTACGCGA GAACCTGCTA 
AAGACTCTCC CCACCTCGCT GTCCTTCCTT GTCAAGTTAG AGCAGCTGGA TCTCGGTGGC 
AATGACCTGG AGGTTCTGCC AGACACTCTG GGAGCGCTCC CCAACCTGCG TGAGCTCTGG 
TTAGACCGGA ACCAGCTCTC AGCCCTGCCT CCCGAGCTGG GCAACCTCCG CCGCCTGGTC 
TGCCTGGATG TGTCGGAGAA CAAGCTGGAG CAGTTGCCCA ACGAGGTCAG CGGCCTGGTG 
GCACTGACCG ACTTGCTGCT GTCGCAGAAC CTGTTGGAGT GCATTCCTGA TGGGATTGGT 
CAGCTGAAGC AGCTCTCCAT CCTCAAGGTG GACCAGAATC GACTGACAGA GGTGACAGAG 
TCCATTGGGG ACTGTGAAAA TCTGTCCGAG CTCATCCTGA CAGAGAACAT GCTGACGGCC 
TTGCCGAAAT CCCTTGGGAA GCTGGCCAAG CTGACCAACC TGAACGTGGA TCGGAACCGG 
CTGACATCCC TCCCGGCCGA GATCGGTGGC TGTGCCAACC TCAACGTGCT GTCCTTGCGG 
GACAATCGCC TGACCCTCCT GCCATCGGAG CTGGCCAACA CCACCGAGCT TCATGTTCTG 
GATGTGGCTG GGAACAGGCT GCAGAACTTG CCGTTTGCTC TGACAAACCT CAACCTGAAA 
GCCCTGTGGC TGGCGGAGAA TCAGTCCCAG CCCATGCTGA AATTCCAAAC AGAGGATGAT 
GAGAAGACAG GAGAGAAAGT TCTCACTTGT TACCTGCTTC CTCAGCAGCC CTCTCCCAGC 
CTGGAGAACC TTCTACAGAA CAGCGTGGAC GAGAGCTGGA CGGACACCAA TTTAAACAGA 
GTCAGTGTGA TTCAGTTCCT GGATGAACCC AAAGCAGATG ATGAAGAAGA GTCTGGAGCT 
GAGAGAAGGG GCTTGCAGCG CAGAGCAACC CCCCATCCCA GCGAGCTGAA GGTGATGAAG 
AAGGTGATTG AAGTTCGGCG CAGTGAGGCC CACGCTGCAA AGCCCAATGC AGACCTGAAT 
TCTCCTCACT CAGAAGAGAA GAGGCTGAGC GCCACGTCCA ACCGCAGCGA GGACTCCCAC 
CTTTCCAACA GCACTGCCTC TGCTGACTGT AGGGCGGAGG AGCAGCAGGA GGAGGCAGAG 
AGGAGTCGGC CAAACGGGCA AGTGCCTGAG ATGCAGGAGC TGGAGAAGGA GGCAAAGTCC 
AGAGTGGACG AGGAGCACAA GGAAGCTGCT GGCCATGAAG AGGAAGATGT GGAAGTGGAA 
TACAATGAGC CCACTGTACA CTTTGCTGAA GACACGCTAA TACGGAGTGA GGATGAAGAT 
GAAGATGAAG AGCGACCGTT CCCAGTGGAG AAGCAGCGGC TCATCCGCAA GGACACCCCT 
CACTATAAGA AACACTTTAA GATAACTAAA TTGCCCAAGC CAGAGGCGGT GGTGGCCCTG 
CTGCAGGGGC TGAACAGCGA GGGGGTCCCC AAGGAGGAAG AGGAGTTGGG AGGCTGCAAC 
AACAACACAG AGCCTGCAGA CCAGGAGGAG GTGTGGGATG AGGAGGACCG GAAGAATGGA 
GCTTTGTCTG CCCAGCCATC GGTGAAGGGG GTGTCGTTTG ATCAAGCCAA TAATCTGCTG 
ATTGAACCTG CTCGCATTGA GGAGGAAGAG TTGACTCTGA CTATCGTACG GCAGACGGGG 
GGGCTGGGCA TCAGCATCGC AGGGGGCAAG GGCTCCACCC CCTATAAGGG CGATGATGAG 
GGCATCTTTA TTTCTCGGGT GTCAGAAGAA GGTCCTGCGG CTCGTGCAGG GGTTCGTGTT 
GGGGACAAGC TCCTGGAGGT GAACGGTGTG TCCCTGCACT CCGCTGAGCA CCACGTGGCA 
GTGGAGGCTC TGCGTGGATC GGGAAGTTCC GTCTCCATGA CTGTTCTGCG GGAGCGGATG 
GTGGAGCCGG AGAATGCCAT CACTGTCACC CCGCTGCGCC CCGAGGATGA CTACAGCCCC 
CGTGAGAGGC GAGGTGGCCT GCGCTTCCCG GAGCGACCCG AGGGGACACC TCCCACCGAG 
AGATACTCCA CGTGCCTCAT GCGCAACGAG AAGGGACTGG GCTTCAGTAT CGCTGGTGGC 
AAAGGCTCCA CTCCTTACCG GGCTGGTGAC ATGGGGATCT TTATCTCCCG GATCGCAGAG 
GGTGGTGCAG CCCATCGGGA CGGGATCCTG CACGTAGGAG ATCGGGTCAT CTCGATCAAT 
GGGGTAGACA TGACAGAAGC CAGGCATGAT CAGGCAGTAG CGCTGCTTAC CGCGTCCTCC 
CCCACCATCG TGCTGCTGGT AGAGAGAGAG GGTGCGGAGC AGCCCGGCGA GGGAGACTGG 
CCCGGGGGGC CACGGGCACG CATGCACTCC CCACCGCCAC CCCCCAGCCA TGGGGAGAGC 
CCGCCCGAGG AGACGCCCGC ACCACAGAGG AACCATCTCT CCAAAGGCCT GGAGGATCAG 
TACCCCATTG AGGAAATTCA CCTGGTGAAA GCAGGTGGTC CCCTGGGGCT CAGCATCGTA 
GGGGGCAGTG ACCACTCAAG CCATCCGTTT GGGATTCATG AACCAGGTGT CTTCATTTCG 
AAGGTCATTC CCCGTGGGCT GGCATCCCGC AGTGGGCTGC GAGTAGGGGA CAGGATCCTG 
GAAGTCAATA GCATTGACCT GCGCCACGCT ACCCACCAGG AAGCGGTGAA CGCCCTGCTC 
TCCAACACCC AGGAGCTGAC CATGTTAGTG AGGCGAGACC CACCACCACC TGGTATGCAG 
GAAATATGCA TTGAGAAGGC TCCAGGAGAA AAGCTGGGCA TCAGCATCCG GGGTGGAGCG 
AAAGGCCATG CTGGAAATCC GTTTGATCCC ACTGACGAGG GCATCTTCAT CTCTAAGGTG 
AGCTCCTCGG GGGCGGCAGC CCGGGACGGC CGCCTGAAGG TGGGGATGCG GATCCTAGAG 
GTGAACCACC AGAGCCTGCT GGGGATGACG CACACGGAAG CGGTGCAGAT GCTGCGCGGG 
GTGGGCGACG CGCTCCTGGT GCTGGTGTGT GACGGCTTCG ACCCCAAGGC TGCAGCTGCC 
ATCGAGATGT CCCCGGGAAT TATTGCAAAC CCTTTCGCTG CTGGTATCGG ACGCAAGAAT 
AGCCTGGAAA GTATCTCTTC CATCGACCGG GATTTAAGCC CTGAGGAACT GGAGATCCTG 
CAGAAGGAGA TGGAAATGGT GAGAGAAGCA ACTCAGTGGG AGAGAGAAGA AATGGAGAAA 
GTGGAGCGGA TGCGTAGGGA GCGGGAGGCA GCCACCCGGC AGCTTGAGGA GGAGGCGGAG 
AACGTCACCA GCGAGCCCTT GAGACTGGAC TATCGGACCC TGGCTGCGCT GCCGAGCAGT 
GGTCTCCAGA GAGTCAACCG CACCGCTCCT GCAGGCGAGT CTGGGATGGG CAATTCCAGG 
CGTGAAACCC CCTATTCTCC TGGTAACCAG CAGGCTTCCA TTAACTTCAT TACATCGCAG 
GATGACAGTA AATCGCCAAA GCCACCCTCA CCACCCTCTC CTGATGAGGT CCCCATCAAT 
GTCAAGCAAG CGTACAAGAC CTTCGCTGCA GTGCCCTTGT CACACCCCCT GCTTGGGACA 
CAGGAATTGC CTGGTCAGAG CGGCACAGAG AATCCCAGAA GTGCCCCGGA GGTGGCTGCG 
CACAGCCCTG GGGGACTGCA CAGCCCTGAG CAGAAGTCCT TCCGTGAGAG GCAGAAGTAT 
TTCGAGATTG AGGTGAAGCA GCAGCAGATG GACAAGCCCC CCAAGAGAGT CTCCTTGGTG 
GGAGAGGACG ACCTGAAGAA GATGAAAGAG GAGGAAGCTC GAAAACTGCA GCAGAAACGT 
ACCCTTCTGC TGGAGGAAGA GGCAGAAGAG GAGGAGATGG CGAAGCAGGC GCCTGAGGTG 
AGCGTGCAGT CCAGTGTCAT CATTGAAGGA GTCGAGTACA AGATTGAGAG ACTCAACGGG 
AGGAGCAGCC AGTCTCCGGC CACTCGGCCC TCCGAGGTCA ACGAGAACAG CAGCTCGCAG 
AGGAATTCAC TGGAAGAGGG GATCAAGGCT GAGCAGAGAA CCAACTCCAT GTCTGGGCTG 
AGTCCCTTGT ACCTTGGAGC AGGGGACGCA GTGGCACCCG TGCGGACGGC CAAAGCTGAA 
CGGCGACACC AGGAGCGGTT GCGAATGCAG AGTCCTGAGC TGCTGAGTGG CCAAGAGAAG 
GAGCTTTCTC CTGCAGAACG TCGTGCTCTG GAGGCGGAGA AACGAGCAAT GTGGAGGGCA 
GCACGATCCT CAGCTCTTGA AGACAGTATT AAACAGTACG AGCAGGACCT GGCCAGGAGG 
CTGTACCAGT CCCGACAGCG GGCACCTGCT CCCGGGGCCG GGCCAGCTCC GATGCCTAGT 
CCCAAGCAGA GTCACGCCTC GGCACCCCCT GGGTCAGGAA GCCATTCCAG GATGAAATCG 
CTTGAACAGG ATGCTCTGAA AGCCCAGATG GTCATTGCCA AGTCCAAGGA GGGGAAGAAG 
CGCAGCCCGC TGGAGCAGCT GGCAGAGTCC CCCTCGCCAG TTCCTACACC GTCACCGACA 
CCACTGGAAG ATTGCAGTCC CAGGAACGTC ACCTCCCCAG GAAGGCTGTC CATGTCTGAA 
AAGAAGTTTG ACTACCGGGA ATTTGCTGCT ATTCCTTCCT CCAAACCCGT TTACGAAATT 
CAGTCGCCTG ATGCTGCTGA TGACCTCAGA TACATCGAGG ACAGGAATAA CTCAGTTCCC 
CAGGAGCAGG ATGGGCAGCC CGAGGAAGAG GACACATTAG GAACTCACGA CTCATCCACA 
CCCACTCCAT CAGCACTTGA AGAAATGGCC CTGTACAGCA GCAAACGCAA GCTCCGACAC 
AGCCGGCGCA GCCTGGAGGC TGCCGTCCCT ACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014801216.1
CDS	6..5381
Translation	MPPLWRCNRHVEALDRRHCSLQAVPEEIYRYSRSLEELLLDANQLRELPKPFFRLLNLRKLGLSDNEIQRLPPEVANFMQLVELDISRNDIPEIPESIKFCKSLEIADFSGNPLSRLPEGFTQLRSLGHLALNDVSLQSLPSDIGNLANLVTLELRENLLKTLPTSLSFLVKLEQLDLGGNDLEVLPDTLGALPNLRELWLDRNQLSALPPELGNLRRLVCLDVSENKLEQLPNEVSGLVALTDLLLSQNLLECIPDGIGQLKQLSILKVDQNRLTEVTESIGDCENLSELILTENMLTALPKSLGKLAKLTNLNVDRNRLTSLPAEIGGCANLNVLSLRDNRLTLLPSELANTTELHVLDVAGNRLQNLPFALTNLNLKALWLAENQSQPMLKFQTEDDEKTGEKVLTCYLLPQQPSPSLENLLQNSVDESWTDTNLNRVSVIQFLDEPKADDEEESGAERRGLQRRATPHPSELKVMKKVIEVRRSEAHAAKPNADLNSPHSEEKRLSATSNRSEDSHLSNSTASADCRAEEQQEEAERSRPNGQVPEMQELEKEAKSRVDEEHKEAAGHEEEDVEVEYNEPTVHFAEDTLIRSEDEDEDEERPFPVEKQRLIRKDTPHYKKHFKITKLPKPEAVVALLQGLNSEGVPKEEEELGGCNNNTEPADQEEVWDEEDRKNGALSAQPSVKGVSFDQANNLLIEPARIEEEELTLTIVRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVRVGDKLLEVNGVSLHSAEHHVAVEALRGSGSSVSMTVLRERMVEPENAITVTPLRPEDDYSPRERRGGLRFPERPEGTPPTERYSTCLMRNEKGLGFSIAGGKGSTPYRAGDMGIFISRIAEGGAAHRDGILHVGDRVISINGVDMTEARHDQAVALLTASSPTIVLLVEREGAEQPGEGDWPGGPRARMHSPPPPPSHGESPPEETPAPQRNHLSKGLEDQYPIEEIHLVKAGGPLGLSIVGGSDHSSHPFGIHEPGVFISKVIPRGLASRSGLRVGDRILEVNSIDLRHATHQEAVNALLSNTQELTMLVRRDPPPPGMQEICIEKAPGEKLGISIRGGAKGHAGNPFDPTDEGIFISKVSSSGAAARDGRLKVGMRILEVNHQSLLGMTHTEAVQMLRGVGDALLVLVCDGFDPKAAAAIEMSPGIIANPFAAGIGRKNSLESISSIDRDLSPEELEILQKEMEMVREATQWEREEMEKVERMRREREAATRQLEEEAENVTSEPLRLDYRTLAALPSSGLQRVNRTAPAGESGMGNSRRETPYSPGNQQASINFITSQDDSKSPKPPSPPSPDEVPINVKQAYKTFAAVPLSHPLLGTQELPGQSGTENPRSAPEVAAHSPGGLHSPEQKSFRERQKYFEIEVKQQQMDKPPKRVSLVGEDDLKKMKEEEARKLQQKRTLLLEEEAEEEEMAKQAPEVSVQSSVIIEGVEYKIERLNGRSSQSPATRPSEVNENSSSQRNSLEEGIKAEQRTNSMSGLSPLYLGAGDAVAPVRTAKAERRHQERLRMQSPELLSGQEKELSPAERRALEAEKRAMWRAARSSALEDSIKQYEQDLARRLYQSRQRAPAPGAGPAPMPSPKQSHASAPPGSGSHSRMKSLEQDALKAQMVIAKSKEGKKRSPLEQLAESPSPVPTPSPTPLEDCSPRNVTSPGRLSMSEKKFDYREFAAIPSSKPVYEIQSPDAADDLRYIEDRNNSVPQEQDGQPEEEDTLGTHDSSTPTPSALEEMALYSSKRKLRHSRRSLEAAVPT

Target ORF information:

RefSeq Version	XM_014945730.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax scribbled planar cell polarity protein (SCRIB), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014945730.1

ORF Insert Sequence:

ATGCCGCCGC TCTGGCGCTG CAACCGGCAC GTGGAGGCGC TGGACCGGCG GCACTGCTCG 
CTGCAGGCCG TGCCCGAGGA GATCTACCGC TACAGCCGCT CGCTGGAGGA GCTGCTGCTG 
GACGCCAACC AGCTGCGGGA GCTGCCCAAG CCTTTCTTCA GGCTTCTGAA CCTGCGCAAG 
CTGGGCCTGA GTGACAATGA GATCCAAAGA CTTCCCCCTG AGGTGGCCAA CTTCATGCAG 
CTGGTGGAAC TGGACATCTC CCGTAACGAC ATTCCAGAAA TTCCTGAAAG CATCAAATTC 
TGCAAATCCT TGGAAATCGC AGACTTCAGT GGGAATCCTC TCTCCAGGCT TCCCGAGGGC 
TTCACGCAGC TTCGGAGCCT GGGCCATTTG GCCCTGAATG ATGTGTCCCT GCAGAGCCTC 
CCCAGTGACA TTGGGAATTT GGCAAACCTG GTGACTCTGG AGCTACGCGA GAACCTGCTA 
AAGACTCTCC CCACCTCGCT GTCCTTCCTT GTCAAGTTAG AGCAGCTGGA TCTCGGTGGC 
AATGACCTGG AGGTTCTGCC AGACACTCTG GGAGCGCTCC CCAACCTGCG TGAGCTCTGG 
TTAGACCGGA ACCAGCTCTC AGCCCTGCCT CCCGAGCTGG GCAACCTCCG CCGCCTGGTC 
TGCCTGGATG TGTCGGAGAA CAAGCTGGAG CAGTTGCCCA ACGAGGTCAG CGGCCTGGTG 
GCACTGACCG ACTTGCTGCT GTCGCAGAAC CTGTTGGAGT GCATTCCTGA TGGGATTGGT 
CAGCTGAAGC AGCTCTCCAT CCTCAAGGTG GACCAGAATC GACTGACAGA GGTGACAGAG 
TCCATTGGGG ACTGTGAAAA TCTGTCCGAG CTCATCCTGA CAGAGAACAT GCTGACGGCC 
TTGCCGAAAT CCCTTGGGAA GCTGGCCAAG CTGACCAACC TGAACGTGGA TCGGAACCGG 
CTGACATCCC TCCCGGCCGA GATCGGTGGC TGTGCCAACC TCAACGTGCT GTCCTTGCGG 
GACAATCGCC TGACCCTCCT GCCATCGGAG CTGGCCAACA CCACCGAGCT TCATGTTCTG 
GATGTGGCTG GGAACAGGCT GCAGAACTTG CCGTTTGCTC TGACAAACCT CAACCTGAAA 
GCCCTGTGGC TGGCGGAGAA TCAGTCCCAG CCCATGCTGA AATTCCAAAC AGAGGATGAT 
GAGAAGACAG GAGAGAAAGT TCTCACTTGT TACCTGCTTC CTCAGCAGCC CTCTCCCAGC 
CTGGAGAACC TTCTACAGAA CAGCGTGGAC GAGAGCTGGA CGGACACCAA TTTAAACAGA 
GTCAGTGTGA TTCAGTTCCT GGATGAACCC AAAGCAGATG ATGAAGAAGA GTCTGGAGCT 
GAGAGAAGGG GCTTGCAGCG CAGAGCAACC CCCCATCCCA GCGAGCTGAA GGTGATGAAG 
AAGGTGATTG AAGTTCGGCG CAGTGAGGCC CACGCTGCAA AGCCCAATGC AGACCTGAAT 
TCTCCTCACT CAGAAGAGAA GAGGCTGAGC GCCACGTCCA ACCGCAGCGA GGACTCCCAC 
CTTTCCAACA GCACTGCCTC TGCTGACTGT AGGGCGGAGG AGCAGCAGGA GGAGGCAGAG 
AGGAGTCGGC CAAACGGGCA AGTGCCTGAG ATGCAGGAGC TGGAGAAGGA GGCAAAGTCC 
AGAGTGGACG AGGAGCACAA GGAAGCTGCT GGCCATGAAG AGGAAGATGT GGAAGTGGAA 
TACAATGAGC CCACTGTACA CTTTGCTGAA GACACGCTAA TACGGAGTGA GGATGAAGAT 
GAAGATGAAG AGCGACCGTT CCCAGTGGAG AAGCAGCGGC TCATCCGCAA GGACACCCCT 
CACTATAAGA AACACTTTAA GATAACTAAA TTGCCCAAGC CAGAGGCGGT GGTGGCCCTG 
CTGCAGGGGC TGAACAGCGA GGGGGTCCCC AAGGAGGAAG AGGAGTTGGG AGGCTGCAAC 
AACAACACAG AGCCTGCAGA CCAGGAGGAG GTGTGGGATG AGGAGGACCG GAAGAATGGA 
GCTTTGTCTG CCCAGCCATC GGTGAAGGGG GTGTCGTTTG ATCAAGCCAA TAATCTGCTG 
ATTGAACCTG CTCGCATTGA GGAGGAAGAG TTGACTCTGA CTATCGTACG GCAGACGGGG 
GGGCTGGGCA TCAGCATCGC AGGGGGCAAG GGCTCCACCC CCTATAAGGG CGATGATGAG 
GGCATCTTTA TTTCTCGGGT GTCAGAAGAA GGTCCTGCGG CTCGTGCAGG GGTTCGTGTT 
GGGGACAAGC TCCTGGAGGT GAACGGTGTG TCCCTGCACT CCGCTGAGCA CCACGTGGCA 
GTGGAGGCTC TGCGTGGATC GGGAAGTTCC GTCTCCATGA CTGTTCTGCG GGAGCGGATG 
GTGGAGCCGG AGAATGCCAT CACTGTCACC CCGCTGCGCC CCGAGGATGA CTACAGCCCC 
CGTGAGAGGC GAGGTGGCCT GCGCTTCCCG GAGCGACCCG AGGGGACACC TCCCACCGAG 
AGATACTCCA CGTGCCTCAT GCGCAACGAG AAGGGACTGG GCTTCAGTAT CGCTGGTGGC 
AAAGGCTCCA CTCCTTACCG GGCTGGTGAC ATGGGGATCT TTATCTCCCG GATCGCAGAG 
GGTGGTGCAG CCCATCGGGA CGGGATCCTG CACGTAGGAG ATCGGGTCAT CTCGATCAAT 
GGGGTAGACA TGACAGAAGC CAGGCATGAT CAGGCAGTAG CGCTGCTTAC CGCGTCCTCC 
CCCACCATCG TGCTGCTGGT AGAGAGAGAG GGTGCGGAGC AGCCCGGCGA GGGAGACTGG 
CCCGGGGGGC CACGGGCACG CATGCACTCC CCACCGCCAC CCCCCAGCCA TGGGGAGAGC 
CCGCCCGAGG AGACGCCCGC ACCACAGAGG AACCATCTCT CCAAAGGCCT GGAGGATCAG 
TACCCCATTG AGGAAATTCA CCTGGTGAAA GCAGGTGGTC CCCTGGGGCT CAGCATCGTA 
GGGGGCAGTG ACCACTCAAG CCATCCGTTT GGGATTCATG AACCAGGTGT CTTCATTTCG 
AAGGTCATTC CCCGTGGGCT GGCATCCCGC AGTGGGCTGC GAGTAGGGGA CAGGATCCTG 
GAAGTCAATA GCATTGACCT GCGCCACGCT ACCCACCAGG AAGCGGTGAA CGCCCTGCTC 
TCCAACACCC AGGAGCTGAC CATGTTAGTG AGGCGAGACC CACCACCACC TGGTATGCAG 
GAAATATGCA TTGAGAAGGC TCCAGGAGAA AAGCTGGGCA TCAGCATCCG GGGTGGAGCG 
AAAGGCCATG CTGGAAATCC GTTTGATCCC ACTGACGAGG GCATCTTCAT CTCTAAGGTG 
AGCTCCTCGG GGGCGGCAGC CCGGGACGGC CGCCTGAAGG TGGGGATGCG GATCCTAGAG 
GTGAACCACC AGAGCCTGCT GGGGATGACG CACACGGAAG CGGTGCAGAT GCTGCGCGGG 
GTGGGCGACG CGCTCCTGGT GCTGGTGTGT GACGGCTTCG ACCCCAAGGC TGCAGCTGCC 
ATCGAGATGT CCCCGGGAAT TATTGCAAAC CCTTTCGCTG CTGGTATCGG ACGCAAGAAT 
AGCCTGGAAA GTATCTCTTC CATCGACCGG GATTTAAGCC CTGAGGAACT GGAGATCCTG 
CAGAAGGAGA TGGAAATGGT GAGAGAAGCA ACTCAGTGGG AGAGAGAAGA AATGGAGAAA 
GTGGAGCGGA TGCGTAGGGA GCGGGAGGCA GCCACCCGGC AGCTTGAGGA GGAGGCGGAG 
AACGTCACCA GCGAGCCCTT GAGACTGGAC TATCGGACCC TGGCTGCGCT GCCGAGCAGT 
GGTCTCCAGA GAGTCAACCG CACCGCTCCT GCAGGCGAGT CTGGGATGGG CAATTCCAGG 
CGTGAAACCC CCTATTCTCC TGGTAACCAG CAGGCTTCCA TTAACTTCAT TACATCGCAG 
GATGACAGTA AATCGCCAAA GCCACCCTCA CCACCCTCTC CTGATGAGGT CCCCATCAAT 
GTCAAGCAAG CGTACAAGAC CTTCGCTGCA GTGCCCTTGT CACACCCCCT GCTTGGGACA 
CAGGAATTGC CTGGTCAGAG CGGCACAGAG AATCCCAGAA GTGCCCCGGA GGTGGCTGCG 
CACAGCCCTG GGGGACTGCA CAGCCCTGAG CAGAAGTCCT TCCGTGAGAG GCAGAAGTAT 
TTCGAGATTG AGGTGAAGCA GCAGCAGATG GACAAGCCCC CCAAGAGAGT CTCCTTGGTG 
GGAGAGGACG ACCTGAAGAA GATGAAAGAG GAGGAAGCTC GAAAACTGCA GCAGAAACGT 
ACCCTTCTGC TGGAGGAAGA GGCAGAAGAG GAGGAGATGG CGAAGCAGGC GCCTGAGGTG 
AGCGTGCAGT CCAGTGTCAT CATTGAAGGA GTCGAGTACA AGATTGAGAG ACTCAACGGG 
AGGAGCAGCC AGTCTCCGGC CACTCGGCCC TCCGAGGTCA ACGAGAACAG CAGCTCGCAG 
AGGAATTCAC TGGAAGAGGG GATCAAGGCT GAGCAGAGAA CCAACTCCAT GTCTGGGCTG 
AGTCCCTTGT ACCTTGGAGC AGGGGACGCA GTGGCACCCG TGCGGACGGC CAAAGCTGAA 
CGGCGACACC AGGAGCGGTT GCGAATGCAG AGTCCTGAGC TGCTGAGTGG CCAAGAGAAG 
GAGCTTTCTC CTGCAGAACG TCGTGCTCTG GAGGCGGAGA AACGAGCAAT GTGGAGGGCA 
GCACGATCCT CAGCTCTTGA AGACAGTATT AAACAGTACG AGCAGGACCT GGCCAGGAGG 
CTGTACCAGT CCCGACAGCG GGCACCTGCT CCCGGGGCCG GGCCAGCTCC GATGCCTAGT 
CCCAAGCAGA GTCACGCCTC GGCACCCCCT GGGTCAGGAA GCCATTCCAG GATGAAATCG 
CTTGAACAGG ATGCTCTGAA AGCCCAGATG GTCATTGCCA AGTCCAAGGA GGGGAAGAAG 
CGCAGCCCGC TGGAGCAGCT GGCAGAGTCC CCCTCGCCAG TTCCTACACC GTCACCGACA 
CCACTGGAAG ATTGCAGTCC CAGGAACGTC ACCTCCCCAG GAAGGCTGTC CATGTCTGAA 
AAGAAGTTTG ACTACCGGGA ATTTGCTGCT ATTCCTTCCT CCAAACCCGT TTACGAAATT 
CAGTCGCCTG ATGCTGCTGA TGACCTCAGA TACATCGAGG ACAGGAATAA CTCAGTTCCC 
CAGGAGCAGG ATGGGCAGCC CGAGGAAGAG GACACATTAG GAACTCACGA CTCATCCACA 
CCCACTCCAT CAGCACTTGA AGAAATGGCC CTGTACAGCA GCAAACGCAA GCTCCGACAC 
AGCCGGCGCA GCCTGGAGGC TGCCGTCCCT ACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCRIB ( XM_014945731.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014801217.1 Calidris pugnax scribbled planar cell polarity protein (SCRIB), transcript variant X17, mRNA.

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CloneID	OCa67763
Clone ID Related Accession (Same CDS sequence)	XM_014945731.1
Accession Version	XM_014945731.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5361bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	protein scribble homolog isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090871.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCCGC TCTGGCGCTG CAACCGGCAC GTGGAGGCGC TGGACCGGCG GCACTGCTCG 
CTGCAGGCCG TGCCCGAGGA GATCTACCGC TACAGCCGCT CGCTGGAGGA GCTGCTGCTG 
GACGCCAACC AGCTGCGGGA GCTGCCCAAG CCTTTCTTCA GGCTTCTGAA CCTGCGCAAG 
CTGGGCCTGA GTGACAATGA GATCCAAAGA CTTCCCCCTG AGGTGGCCAA CTTCATGCAG 
CTGGTGGAAC TGGACATCTC CCGTAACGAC ATTCCAGAAA TTCCTGAAAG CATCAAATTC 
TGCAAATCCT TGGAAATCGC AGACTTCAGT GGGAATCCTC TCTCCAGGCT TCCCGAGGGC 
TTCACGCAGC TTCGGAGCCT GGGCCATTTG GCCCTGAATG ATGTGTCCCT GCAGAGCCTC 
CCCAGTGACA TTGGGAATTT GGCAAACCTG GTGACTCTGG AGCTACGCGA GAACCTGCTA 
AAGACTCTCC CCACCTCGCT GTCCTTCCTT GTCAAGTTAG AGCAGCTGGA TCTCGGTGGC 
AATGACCTGG AGGTTCTGCC AGACACTCTG GGAGCGCTCC CCAACCTGCG TGAGCTCTGG 
TTAGACCGGA ACCAGCTCTC AGCCCTGCCT CCCGAGCTGG GCAACCTCCG CCGCCTGGTC 
TGCCTGGATG TGTCGGAGAA CAAGCTGGAG CAGTTGCCCA ACGAGGTCAG CGGCCTGGTG 
GCACTGACCG ACTTGCTGCT GTCGCAGAAC CTGTTGGAGT GCATTCCTGA TGGGATTGGT 
CAGCTGAAGC AGCTCTCCAT CCTCAAGGTG GACCAGAATC GACTGACAGA GGTGACAGAG 
TCCATTGGGG ACTGTGAAAA TCTGTCCGAG CTCATCCTGA CAGAGAACAT GCTGACGGCC 
TTGCCGAAAT CCCTTGGGAA GCTGGCCAAG CTGACCAACC TGAACGTGGA TCGGAACCGG 
CTGACATCCC TCCCGGCCGA GATCGGTGGC TGTGCCAACC TCAACGTGCT GTCCTTGCGG 
GACAATCGCC TGACCCTCCT GCCATCGGAG CTGGCCAACA CCACCGAGCT TCATGTTCTG 
GATGTGGCTG GGAACAGGCT GCAGAACTTG CCGTTTGCTC TGACAAACCT CAACCTGAAA 
GCCCTGTGGC TGGCGGAGAA TCAGTCCCAG CCCATGCTGA AATTCCAAAC AGAGGATGAT 
GAGAAGACAG GAGAGAAAGT TCTCACTTGT TACCTGCTTC CTCAGCAGCC CTCTCCCAGC 
CTGGAGAACC TTCTACAGAA CAGCGTGGAC GAGAGCTGGA CGGACACCAA TTTAAACAGA 
GTCAGTGTGA TTCAGTTCCT GGATGAACCC AAAGCAGATG ATGAAGAAGA GTCTGGAGCT 
GAGAGAAGGG GCTTGCAGCG CAGAGCAACC CCCCATCCCA GCGAGCTGAA GGTGATGAAG 
AAGGTGATTG AAGTTCGGCG CAGTGAGGCC CACGCTGCAA AGCCCAATGC AGACCTGAAT 
TCTCCTCACT CAGAAGAGAA GAGGCTGAGC GCCACGTCCA ACCGCAGCGA GGACTCCCAC 
CTTTCCAACA GCACTGCCTC TGCTGACTGT AGGGCGGAGG AGCAGCAGGA GGAGGCAGAG 
AGGAGTCGGC CAAACGGGCA AGTGCCTGAG ATGCAGGAGC TGGAGAAGGA GGCAAAGTCC 
AGAGTGGACG AGGAGCACAA GGAAGCTGCT GGCCATGAAG AGGAAGATGT GGAAGTGGAA 
TACAATGAGC CCACTGTACA CTTTGCTGAA GACACGCTAA TACGGAGTGA GGATGAAGAT 
GAAGATGAAG AGCGACCGTT CCCAGTGGAG AAGCAGCGGC TCATCCGCAA GGACACCCCT 
CACTATAAGA AACACTTTAA GATAACTAAA TTGCCCAAGC CAGAGGCGGT GGTGGCCCTG 
CTGCAGGGGC TGAACAGCGA GGGGGTCCCC AAGGAGGAAG AGGAGTTGGG AGGCTGCAAC 
AACAACACAG AGCCTGCAGA CCAGGAGGAG GTGTGGGATG AGGAGGACCG GAAGAATGGA 
GCTTTGTCTG CCCAGCCATC GGTGAAGGGG GTGTCGTTTG ATCAAGCCAA TAATCTGCTG 
ATTGAACCTG CTCGCATTGA GGAGGAAGAG TTGACTCTGA CTATCGTACG GCAGACGGGG 
GGGCTGGGCA TCAGCATCGC AGGGGGCAAG GGCTCCACCC CCTATAAGGG CGATGATGAG 
GGCATCTTTA TTTCTCGGGT GTCAGAAGAA GGTCCTGCGG CTCGTGCAGG GGTTCGTGTT 
GGGGACAAGC TCCTGGAGGT GAACGGTGTG TCCCTGCACT CCGCTGAGCA CCACGTGGCA 
GTGGAGGCTC TGCGTGGATC GGGAAGTTCC GTCTCCATGA CTGTTCTGCG GGAGCGGATG 
GTGGAGCCGG AGAATGCCAT CACTGTCACC CCGCTGCGCC CCGAGGATGA CTACAGCCCC 
CGTGAGAGGC GAGGTGGCCT GCGCTTCCCG GAGCGACCCG AGGGGACACC TCCCACCGAG 
AGATACTCCA CGTGCCTCAT GCGCAACGAG AAGGGACTGG GCTTCAGTAT CGCTGGTGGC 
AAAGGCTCCA CTCCTTACCG GGCTGGTGAC ATGGGGATCT TTATCTCCCG GATCGCAGAG 
GGTGGTGCAG CCCATCGGGA CGGGATCCTG CACGTAGGAG ATCGGGTCAT CTCGATCAAT 
GGGGTAGACA TGACAGAAGC CAGGCATGAT CAGGCAGTAG CGCTGCTTAC CGCGTCCTCC 
CCCACCATCG TGCTGCTGGT AGAGAGAGAG GGTGCGGAGC AGCCCGGCGA GGGAGACTGG 
CCCGGGGGGC CACGGGCACG CATGCACTCC CCACCGCCAC CCCCCAGCCA TGGGGAGAGC 
CCGCCCGAGG AGACGCCCGC ACCACAGAGG AACCATCTCT CCAAAGGCCT GGAGGATCAG 
TACCCCATTG AGGAAATTCA CCTGGTGAAA GCAGGTGGTC CCCTGGGGCT CAGCATCGTA 
GGGGGCAGTG ACCACTCAAG CCATCCGTTT GGGATTCATG AACCAGGTGT CTTCATTTCG 
AAGGTCATTC CCCGTGGGCT GGCATCCCGC AGTGGGCTGC GAGTAGGGGA CAGGATCCTG 
GAAGTCAATA GCATTGACCT GCGCCACGCT ACCCACCAGG AAGCGGTGAA CGCCCTGCTC 
TCCAACACCC AGGAGCTGAC CATGTTAGTG AGGCGAGACC CACCACCACC TGGTATGCAG 
GAAATATGCA TTGAGAAGGC TCCAGGAGAA AAGCTGGGCA TCAGCATCCG GGGTGGAGCG 
AAAGGCCATG CTGGAAATCC GTTTGATCCC ACTGACGAGG GCATCTTCAT CTCTAAGGTG 
AGCTCCTCGG GGGCGGCAGC CCGGGACGGC CGCCTGAAGG TGGGGATGCG GATCCTAGAG 
GTGAACCACC AGAGCCTGCT GGGGATGACG CACACGGAAG CGGTGCAGAT GCTGCGCGGG 
GTGGGCGACG CGCTCCTGGT GCTGGTGTGT GACGGCTTCG ACCCCAAGGC TGCAGCTGCC 
ATCGAGATGT CCCCGGGAAT TATTGCAAAC CCTTTCGCTG CTGGTATCGG ACGCAAGAAT 
AGCCTGGAAA GTATCTCTTC CATCGACCGG GATTTAAGCC CTGAGGAACT GGAGATCCTG 
CAGAAGGAGA TGGAAATGGT GAGAGAAGCA ACTCAGTGGG AGAGAGAAGA AATGGAGAAA 
GTGGCTCCTG CAGGCGAGTC TGGGATGGGC AATTCCAGGC GTGAAACCCC CTATTCTCCT 
GGTAACCAGC AGGCTTCCAT TAACTTCATT ACATCGCAGG ATGACAGTAA ATCGACCAAG 
CCAGGCGTCA TTCAGCCTCT TTCCCGAGTG CGACAAAATG CTGCCCCGCA CAATTCGGAG 
GAGGGGGACA GCCCCAATCC CTTCCAGCAG CCCGAGCCGT GCTTCAGTGT CCAGAACTCT 
CCAAAGCCAC CCTCACCACC CTCTCCTGAT GAGGTCCCCA TCAATGTCAA GCAAGCGTAC 
AAGACCTTCG CTGCAGTGCC CTTGTCACAC CCCCTGCTTG GGACACAGGA ATTGCCTGGT 
CAGAGCGGCA CAGAGAATCC CAGAAGTGCC CCGGAGGTGG CTGCGCACAG CCCTGGGGGA 
CTGCACAGCC CTGAGCAGAA GTCCTTCCGT GAGAGGCAGA AGTATTTCGA GATTGAGGTG 
AAGCAGCAGC AGATGGACAA GCCCCCCAAG AGAGTCTCCT TGGTGGGAGA GGACGACCTG 
AAGAAGATGA AAGAGGAGGA AGCTCGAAAA CTGCAGCAGA AACGTACCCT TCTGCTGGAG 
GAAGAGGCAG AAGAGGAGGA GATGGCGAAG CAGGCGCCTG AGGTGAGCGT GCAGTCCAGT 
GTCATCATTG AAGGAGTCGA GTACAAGATT GAGAGACTCA ACGGGAGGAG CAGCCAGTCT 
CCGGCCACTC GGCCCTCCGA GGTCAACGAG AACAGCAGCT CGCAGAGGAA TTCACTGGAA 
GAGGGGATCA AGGCTGAGCA GAGAACCAAC TCCATGTCTG GGCTGAGTCC CTTGTACCTT 
GGAGCAGGGG ACGCAGTGGC ACCCGTGCGG ACGGCCAAAG CTGAACGGCG ACACCAGGAG 
CGGTTGCGAA TGCAGAGTCC TGAGCTGCTG AGTGGCCAAG AGAAGGAGCT TTCTCCTGCA 
GAACGTCGTG CTCTGGAGGC GGAGAAACGA GCAATGTGGA GGGCAGCACG ATCCTCAGCT 
CTTGAAGACA GTATTAAACA GTACGAGCAG GACCTGGCCA GGAGGCTGTA CCAGTCCCGA 
CAGCGGGCAC CTGCTCCCGG GGCCGGGCCA GCTCCGATGC CTAGTCCCAA GCAGAGTCAC 
GCCTCGGCAC CCCCTGGGTC AGGAAGCCAT TCCAGGATGA AATCGCTTGA ACAGGATGCT 
CTGAAAGCCC AGATGGTCAT TGCCAAGTCC AAGGAGGGGA AGAAGCGCAG CCCGCTGGAG 
CAGCTGGCAG AGTCCCCCTC GCCAGTTCCT ACACCGTCAC CGACACCACT GGAAGATTGC 
AGTCCCAGGA ACGTCACCTC CCCAGGAAGG CTGTCCATGT CTGAAAAGAA GTTTGACTAC 
CGGGAATTTG CTGCTATTCC TTCCTCCAAA CCCGTTTACG AAATTCAGTC GCCTGATGCT 
GCTGATGACC TCAGATACAT CGAGGACAGG AATAACTCAG TTCCCCAGGA GCAGGATGGG 
CAGCCCGAGG AAGAGGACAC ATTAGGAACT CACGACTCAT CCACACCCAC TCCATCAGCA 
CTTGAAGAAA TGGCCCTGTA CAGCAGCAAA CGCAAGCTCC GACACAGCCG GCGCAGCCTG 
GAGGCTGCCG TCCCTACGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014801217.1
CDS	6..5366
Translation	MPPLWRCNRHVEALDRRHCSLQAVPEEIYRYSRSLEELLLDANQLRELPKPFFRLLNLRKLGLSDNEIQRLPPEVANFMQLVELDISRNDIPEIPESIKFCKSLEIADFSGNPLSRLPEGFTQLRSLGHLALNDVSLQSLPSDIGNLANLVTLELRENLLKTLPTSLSFLVKLEQLDLGGNDLEVLPDTLGALPNLRELWLDRNQLSALPPELGNLRRLVCLDVSENKLEQLPNEVSGLVALTDLLLSQNLLECIPDGIGQLKQLSILKVDQNRLTEVTESIGDCENLSELILTENMLTALPKSLGKLAKLTNLNVDRNRLTSLPAEIGGCANLNVLSLRDNRLTLLPSELANTTELHVLDVAGNRLQNLPFALTNLNLKALWLAENQSQPMLKFQTEDDEKTGEKVLTCYLLPQQPSPSLENLLQNSVDESWTDTNLNRVSVIQFLDEPKADDEEESGAERRGLQRRATPHPSELKVMKKVIEVRRSEAHAAKPNADLNSPHSEEKRLSATSNRSEDSHLSNSTASADCRAEEQQEEAERSRPNGQVPEMQELEKEAKSRVDEEHKEAAGHEEEDVEVEYNEPTVHFAEDTLIRSEDEDEDEERPFPVEKQRLIRKDTPHYKKHFKITKLPKPEAVVALLQGLNSEGVPKEEEELGGCNNNTEPADQEEVWDEEDRKNGALSAQPSVKGVSFDQANNLLIEPARIEEEELTLTIVRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVRVGDKLLEVNGVSLHSAEHHVAVEALRGSGSSVSMTVLRERMVEPENAITVTPLRPEDDYSPRERRGGLRFPERPEGTPPTERYSTCLMRNEKGLGFSIAGGKGSTPYRAGDMGIFISRIAEGGAAHRDGILHVGDRVISINGVDMTEARHDQAVALLTASSPTIVLLVEREGAEQPGEGDWPGGPRARMHSPPPPPSHGESPPEETPAPQRNHLSKGLEDQYPIEEIHLVKAGGPLGLSIVGGSDHSSHPFGIHEPGVFISKVIPRGLASRSGLRVGDRILEVNSIDLRHATHQEAVNALLSNTQELTMLVRRDPPPPGMQEICIEKAPGEKLGISIRGGAKGHAGNPFDPTDEGIFISKVSSSGAAARDGRLKVGMRILEVNHQSLLGMTHTEAVQMLRGVGDALLVLVCDGFDPKAAAAIEMSPGIIANPFAAGIGRKNSLESISSIDRDLSPEELEILQKEMEMVREATQWEREEMEKVAPAGESGMGNSRRETPYSPGNQQASINFITSQDDSKSTKPGVIQPLSRVRQNAAPHNSEEGDSPNPFQQPEPCFSVQNSPKPPSPPSPDEVPINVKQAYKTFAAVPLSHPLLGTQELPGQSGTENPRSAPEVAAHSPGGLHSPEQKSFRERQKYFEIEVKQQQMDKPPKRVSLVGEDDLKKMKEEEARKLQQKRTLLLEEEAEEEEMAKQAPEVSVQSSVIIEGVEYKIERLNGRSSQSPATRPSEVNENSSSQRNSLEEGIKAEQRTNSMSGLSPLYLGAGDAVAPVRTAKAERRHQERLRMQSPELLSGQEKELSPAERRALEAEKRAMWRAARSSALEDSIKQYEQDLARRLYQSRQRAPAPGAGPAPMPSPKQSHASAPPGSGSHSRMKSLEQDALKAQMVIAKSKEGKKRSPLEQLAESPSPVPTPSPTPLEDCSPRNVTSPGRLSMSEKKFDYREFAAIPSSKPVYEIQSPDAADDLRYIEDRNNSVPQEQDGQPEEEDTLGTHDSSTPTPSALEEMALYSSKRKLRHSRRSLEAAVPT

Target ORF information:

RefSeq Version	XM_014945731.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax scribbled planar cell polarity protein (SCRIB), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014945731.1

ORF Insert Sequence:

ATGCCGCCGC TCTGGCGCTG CAACCGGCAC GTGGAGGCGC TGGACCGGCG GCACTGCTCG 
CTGCAGGCCG TGCCCGAGGA GATCTACCGC TACAGCCGCT CGCTGGAGGA GCTGCTGCTG 
GACGCCAACC AGCTGCGGGA GCTGCCCAAG CCTTTCTTCA GGCTTCTGAA CCTGCGCAAG 
CTGGGCCTGA GTGACAATGA GATCCAAAGA CTTCCCCCTG AGGTGGCCAA CTTCATGCAG 
CTGGTGGAAC TGGACATCTC CCGTAACGAC ATTCCAGAAA TTCCTGAAAG CATCAAATTC 
TGCAAATCCT TGGAAATCGC AGACTTCAGT GGGAATCCTC TCTCCAGGCT TCCCGAGGGC 
TTCACGCAGC TTCGGAGCCT GGGCCATTTG GCCCTGAATG ATGTGTCCCT GCAGAGCCTC 
CCCAGTGACA TTGGGAATTT GGCAAACCTG GTGACTCTGG AGCTACGCGA GAACCTGCTA 
AAGACTCTCC CCACCTCGCT GTCCTTCCTT GTCAAGTTAG AGCAGCTGGA TCTCGGTGGC 
AATGACCTGG AGGTTCTGCC AGACACTCTG GGAGCGCTCC CCAACCTGCG TGAGCTCTGG 
TTAGACCGGA ACCAGCTCTC AGCCCTGCCT CCCGAGCTGG GCAACCTCCG CCGCCTGGTC 
TGCCTGGATG TGTCGGAGAA CAAGCTGGAG CAGTTGCCCA ACGAGGTCAG CGGCCTGGTG 
GCACTGACCG ACTTGCTGCT GTCGCAGAAC CTGTTGGAGT GCATTCCTGA TGGGATTGGT 
CAGCTGAAGC AGCTCTCCAT CCTCAAGGTG GACCAGAATC GACTGACAGA GGTGACAGAG 
TCCATTGGGG ACTGTGAAAA TCTGTCCGAG CTCATCCTGA CAGAGAACAT GCTGACGGCC 
TTGCCGAAAT CCCTTGGGAA GCTGGCCAAG CTGACCAACC TGAACGTGGA TCGGAACCGG 
CTGACATCCC TCCCGGCCGA GATCGGTGGC TGTGCCAACC TCAACGTGCT GTCCTTGCGG 
GACAATCGCC TGACCCTCCT GCCATCGGAG CTGGCCAACA CCACCGAGCT TCATGTTCTG 
GATGTGGCTG GGAACAGGCT GCAGAACTTG CCGTTTGCTC TGACAAACCT CAACCTGAAA 
GCCCTGTGGC TGGCGGAGAA TCAGTCCCAG CCCATGCTGA AATTCCAAAC AGAGGATGAT 
GAGAAGACAG GAGAGAAAGT TCTCACTTGT TACCTGCTTC CTCAGCAGCC CTCTCCCAGC 
CTGGAGAACC TTCTACAGAA CAGCGTGGAC GAGAGCTGGA CGGACACCAA TTTAAACAGA 
GTCAGTGTGA TTCAGTTCCT GGATGAACCC AAAGCAGATG ATGAAGAAGA GTCTGGAGCT 
GAGAGAAGGG GCTTGCAGCG CAGAGCAACC CCCCATCCCA GCGAGCTGAA GGTGATGAAG 
AAGGTGATTG AAGTTCGGCG CAGTGAGGCC CACGCTGCAA AGCCCAATGC AGACCTGAAT 
TCTCCTCACT CAGAAGAGAA GAGGCTGAGC GCCACGTCCA ACCGCAGCGA GGACTCCCAC 
CTTTCCAACA GCACTGCCTC TGCTGACTGT AGGGCGGAGG AGCAGCAGGA GGAGGCAGAG 
AGGAGTCGGC CAAACGGGCA AGTGCCTGAG ATGCAGGAGC TGGAGAAGGA GGCAAAGTCC 
AGAGTGGACG AGGAGCACAA GGAAGCTGCT GGCCATGAAG AGGAAGATGT GGAAGTGGAA 
TACAATGAGC CCACTGTACA CTTTGCTGAA GACACGCTAA TACGGAGTGA GGATGAAGAT 
GAAGATGAAG AGCGACCGTT CCCAGTGGAG AAGCAGCGGC TCATCCGCAA GGACACCCCT 
CACTATAAGA AACACTTTAA GATAACTAAA TTGCCCAAGC CAGAGGCGGT GGTGGCCCTG 
CTGCAGGGGC TGAACAGCGA GGGGGTCCCC AAGGAGGAAG AGGAGTTGGG AGGCTGCAAC 
AACAACACAG AGCCTGCAGA CCAGGAGGAG GTGTGGGATG AGGAGGACCG GAAGAATGGA 
GCTTTGTCTG CCCAGCCATC GGTGAAGGGG GTGTCGTTTG ATCAAGCCAA TAATCTGCTG 
ATTGAACCTG CTCGCATTGA GGAGGAAGAG TTGACTCTGA CTATCGTACG GCAGACGGGG 
GGGCTGGGCA TCAGCATCGC AGGGGGCAAG GGCTCCACCC CCTATAAGGG CGATGATGAG 
GGCATCTTTA TTTCTCGGGT GTCAGAAGAA GGTCCTGCGG CTCGTGCAGG GGTTCGTGTT 
GGGGACAAGC TCCTGGAGGT GAACGGTGTG TCCCTGCACT CCGCTGAGCA CCACGTGGCA 
GTGGAGGCTC TGCGTGGATC GGGAAGTTCC GTCTCCATGA CTGTTCTGCG GGAGCGGATG 
GTGGAGCCGG AGAATGCCAT CACTGTCACC CCGCTGCGCC CCGAGGATGA CTACAGCCCC 
CGTGAGAGGC GAGGTGGCCT GCGCTTCCCG GAGCGACCCG AGGGGACACC TCCCACCGAG 
AGATACTCCA CGTGCCTCAT GCGCAACGAG AAGGGACTGG GCTTCAGTAT CGCTGGTGGC 
AAAGGCTCCA CTCCTTACCG GGCTGGTGAC ATGGGGATCT TTATCTCCCG GATCGCAGAG 
GGTGGTGCAG CCCATCGGGA CGGGATCCTG CACGTAGGAG ATCGGGTCAT CTCGATCAAT 
GGGGTAGACA TGACAGAAGC CAGGCATGAT CAGGCAGTAG CGCTGCTTAC CGCGTCCTCC 
CCCACCATCG TGCTGCTGGT AGAGAGAGAG GGTGCGGAGC AGCCCGGCGA GGGAGACTGG 
CCCGGGGGGC CACGGGCACG CATGCACTCC CCACCGCCAC CCCCCAGCCA TGGGGAGAGC 
CCGCCCGAGG AGACGCCCGC ACCACAGAGG AACCATCTCT CCAAAGGCCT GGAGGATCAG 
TACCCCATTG AGGAAATTCA CCTGGTGAAA GCAGGTGGTC CCCTGGGGCT CAGCATCGTA 
GGGGGCAGTG ACCACTCAAG CCATCCGTTT GGGATTCATG AACCAGGTGT CTTCATTTCG 
AAGGTCATTC CCCGTGGGCT GGCATCCCGC AGTGGGCTGC GAGTAGGGGA CAGGATCCTG 
GAAGTCAATA GCATTGACCT GCGCCACGCT ACCCACCAGG AAGCGGTGAA CGCCCTGCTC 
TCCAACACCC AGGAGCTGAC CATGTTAGTG AGGCGAGACC CACCACCACC TGGTATGCAG 
GAAATATGCA TTGAGAAGGC TCCAGGAGAA AAGCTGGGCA TCAGCATCCG GGGTGGAGCG 
AAAGGCCATG CTGGAAATCC GTTTGATCCC ACTGACGAGG GCATCTTCAT CTCTAAGGTG 
AGCTCCTCGG GGGCGGCAGC CCGGGACGGC CGCCTGAAGG TGGGGATGCG GATCCTAGAG 
GTGAACCACC AGAGCCTGCT GGGGATGACG CACACGGAAG CGGTGCAGAT GCTGCGCGGG 
GTGGGCGACG CGCTCCTGGT GCTGGTGTGT GACGGCTTCG ACCCCAAGGC TGCAGCTGCC 
ATCGAGATGT CCCCGGGAAT TATTGCAAAC CCTTTCGCTG CTGGTATCGG ACGCAAGAAT 
AGCCTGGAAA GTATCTCTTC CATCGACCGG GATTTAAGCC CTGAGGAACT GGAGATCCTG 
CAGAAGGAGA TGGAAATGGT GAGAGAAGCA ACTCAGTGGG AGAGAGAAGA AATGGAGAAA 
GTGGCTCCTG CAGGCGAGTC TGGGATGGGC AATTCCAGGC GTGAAACCCC CTATTCTCCT 
GGTAACCAGC AGGCTTCCAT TAACTTCATT ACATCGCAGG ATGACAGTAA ATCGACCAAG 
CCAGGCGTCA TTCAGCCTCT TTCCCGAGTG CGACAAAATG CTGCCCCGCA CAATTCGGAG 
GAGGGGGACA GCCCCAATCC CTTCCAGCAG CCCGAGCCGT GCTTCAGTGT CCAGAACTCT 
CCAAAGCCAC CCTCACCACC CTCTCCTGAT GAGGTCCCCA TCAATGTCAA GCAAGCGTAC 
AAGACCTTCG CTGCAGTGCC CTTGTCACAC CCCCTGCTTG GGACACAGGA ATTGCCTGGT 
CAGAGCGGCA CAGAGAATCC CAGAAGTGCC CCGGAGGTGG CTGCGCACAG CCCTGGGGGA 
CTGCACAGCC CTGAGCAGAA GTCCTTCCGT GAGAGGCAGA AGTATTTCGA GATTGAGGTG 
AAGCAGCAGC AGATGGACAA GCCCCCCAAG AGAGTCTCCT TGGTGGGAGA GGACGACCTG 
AAGAAGATGA AAGAGGAGGA AGCTCGAAAA CTGCAGCAGA AACGTACCCT TCTGCTGGAG 
GAAGAGGCAG AAGAGGAGGA GATGGCGAAG CAGGCGCCTG AGGTGAGCGT GCAGTCCAGT 
GTCATCATTG AAGGAGTCGA GTACAAGATT GAGAGACTCA ACGGGAGGAG CAGCCAGTCT 
CCGGCCACTC GGCCCTCCGA GGTCAACGAG AACAGCAGCT CGCAGAGGAA TTCACTGGAA 
GAGGGGATCA AGGCTGAGCA GAGAACCAAC TCCATGTCTG GGCTGAGTCC CTTGTACCTT 
GGAGCAGGGG ACGCAGTGGC ACCCGTGCGG ACGGCCAAAG CTGAACGGCG ACACCAGGAG 
CGGTTGCGAA TGCAGAGTCC TGAGCTGCTG AGTGGCCAAG AGAAGGAGCT TTCTCCTGCA 
GAACGTCGTG CTCTGGAGGC GGAGAAACGA GCAATGTGGA GGGCAGCACG ATCCTCAGCT 
CTTGAAGACA GTATTAAACA GTACGAGCAG GACCTGGCCA GGAGGCTGTA CCAGTCCCGA 
CAGCGGGCAC CTGCTCCCGG GGCCGGGCCA GCTCCGATGC CTAGTCCCAA GCAGAGTCAC 
GCCTCGGCAC CCCCTGGGTC AGGAAGCCAT TCCAGGATGA AATCGCTTGA ACAGGATGCT 
CTGAAAGCCC AGATGGTCAT TGCCAAGTCC AAGGAGGGGA AGAAGCGCAG CCCGCTGGAG 
CAGCTGGCAG AGTCCCCCTC GCCAGTTCCT ACACCGTCAC CGACACCACT GGAAGATTGC 
AGTCCCAGGA ACGTCACCTC CCCAGGAAGG CTGTCCATGT CTGAAAAGAA GTTTGACTAC 
CGGGAATTTG CTGCTATTCC TTCCTCCAAA CCCGTTTACG AAATTCAGTC GCCTGATGCT 
GCTGATGACC TCAGATACAT CGAGGACAGG AATAACTCAG TTCCCCAGGA GCAGGATGGG 
CAGCCCGAGG AAGAGGACAC ATTAGGAACT CACGACTCAT CCACACCCAC TCCATCAGCA 
CTTGAAGAAA TGGCCCTGTA CAGCAGCAAA CGCAAGCTCC GACACAGCCG GCGCAGCCTG 
GAGGCTGCCG TCCCTACGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCRIB ( XM_014945732.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014801218.1 Calidris pugnax scribbled planar cell polarity protein (SCRIB), transcript variant X18, mRNA.

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CloneID	OCa67764
Clone ID Related Accession (Same CDS sequence)	XM_014945732.1
Accession Version	XM_014945732.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5355bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	protein scribble homolog isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090871.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCCGC TCTGGCGCTG CAACCGGCAC GTGGAGGCGC TGGACCGGCG GCACTGCTCG 
CTGCAGGCCG TGCCCGAGGA GATCTACCGC TACAGCCGCT CGCTGGAGGA GCTGCTGCTG 
GACGCCAACC AGCTGCGGGA GCTGCCCAAG CCTTTCTTCA GGCTTCTGAA CCTGCGCAAG 
CTGGGCCTGA GTGACAATGA GATCCAAAGA CTTCCCCCTG AGGTGGCCAA CTTCATGCAG 
CTGGTGGAAC TGGACATCTC CCGTAACGAC ATTCCAGAAA TTCCTGAAAG CATCAAATTC 
TGCAAATCCT TGGAAATCGC AGACTTCAGT GGGAATCCTC TCTCCAGGCT TCCCGAGGGC 
TTCACGCAGC TTCGGAGCCT GGGCCATTTG GCCCTGAATG ATGTGTCCCT GCAGAGCCTC 
CCCAGTGACA TTGGGAATTT GGCAAACCTG GTGACTCTGG AGCTACGCGA GAACCTGCTA 
AAGACTCTCC CCACCTCGCT GTCCTTCCTT GTCAAGTTAG AGCAGCTGGA TCTCGGTGGC 
AATGACCTGG AGGTTCTGCC AGACACTCTG GGAGCGCTCC CCAACCTGCG TGAGCTCTGG 
TTAGACCGGA ACCAGCTCTC AGCCCTGCCT CCCGAGCTGG GCAACCTCCG CCGCCTGGTC 
TGCCTGGATG TGTCGGAGAA CAAGCTGGAG CAGTTGCCCA ACGAGGTCAG CGGCCTGGTG 
GCACTGACCG ACTTGCTGCT GTCGCAGAAC CTGTTGGAGT GCATTCCTGA TGGGATTGGT 
CAGCTGAAGC AGCTCTCCAT CCTCAAGGTG GACCAGAATC GACTGACAGA GGTGACAGAG 
TCCATTGGGG ACTGTGAAAA TCTGTCCGAG CTCATCCTGA CAGAGAACAT GCTGACGGCC 
TTGCCGAAAT CCCTTGGGAA GCTGGCCAAG CTGACCAACC TGAACGTGGA TCGGAACCGG 
CTGACATCCC TCCCGGCCGA GATCGGTGGC TGTGCCAACC TCAACGTGCT GTCCTTGCGG 
GACAATCGCC TGACCCTCCT GCCATCGGAG CTGGCCAACA CCACCGAGCT TCATGTTCTG 
GATGTGGCTG GGAACAGGCT GCAGAACTTG CCGTTTGCTC TGACAAACCT CAACCTGAAA 
GCCCTGTGGC TGGCGGAGAA TCAGTCCCAG CCCATGCTGA AATTCCAAAC AGAGGATGAT 
GAGAAGACAG GAGAGAAAGT TCTCACTTGT TACCTGCTTC CTCAGCAGCC CTCTCCCAGC 
CTGGAGAACC TTCTACAGAA CAGCGTGGAC GAGAGCTGGA CGGACACCAA TTTAAACAGA 
GTCAGTGTGA TTCAGTTCCT GGATGAACCC AAAGCAGATG ATGAAGAAGA GTCTGGAGCT 
GAGAGAAGGG GCTTGCAGCG CAGAGCAACC CCCCATCCCA GCGAGCTGAA GGTGATGAAG 
AAGGTGATTG AAGTTCGGCG CAGTGAGGCC CACGCTGCAA AGCCCAATGC AGACCTGAAT 
TCTCCTCACT CAGAAGAGAA GAGGCTGAGC GCCACGTCCA ACCGCAGCGA GGACTCCCAC 
CTTTCCAACA GCACTGCCTC TGCTGACTGT AGGGCGGAGG AGCAGCAGGA GGAGGCAGAG 
AGGAGTCGGC CAAACGGGCA AGTGCCTGAG ATGCAGGAGC TGGAGAAGGA GGCAAAGTCC 
AGAGTGGACG AGGAGCACAA GGAAGCTGCT GGCCATGAAG AGGAAGATGT GGAAGTGGAA 
TACAATGAGC CCACTGTACA CTTTGCTGAA GACACGCTAA TACGGAGTGA GGATGAAGAT 
GAAGATGAAG AGCGACCGTT CCCAGTGGAG AAGCAGCGGC TCATCCGCAA GGACACCCCT 
CACTATAAGA AACACTTTAA GATAACTAAA TTGCCCAAGC CAGAGGCGGT GGTGGCCCTG 
CTGCAGGGGC TGAACAGCGA GGGGGTCCCC AAGGAGGAAG AGGAGTTGGG AGGCTGCAAC 
AACAACACAG AGCCTGCAGA CCAGGAGGAG GTGTGGGATG AGGAGGACCG GAAGAATGGA 
GCTTTGTCTG CCCAGCCATC GGTGAAGGGG GTGTCGTTTG ATCAAGCCAA TAATCTGCTG 
ATTGAACCTG CTCGCATTGA GGAGGAAGAG TTGACTCTGA CTATCGTACG GCAGACGGGG 
GGGCTGGGCA TCAGCATCGC AGGGGGCAAG GGCTCCACCC CCTATAAGGG CGATGATGAG 
GGCATCTTTA TTTCTCGGGT GTCAGAAGAA GGTCCTGCGG CTCGTGCAGG GGTTCGTGTT 
GGGGACAAGC TCCTGGAGGT GAACGGTGTG TCCCTGCACT CCGCTGAGCA CCACGTGGCA 
GTGGAGGCTC TGCGTGGATC GGGAAGTTCC GTCTCCATGA CTGTTCTGCG GGAGCGGATG 
GTGGAGCCGG AGAATGCCAT CACTGTCACC CCGCTGCGCC CCGAGGATGA CTACAGCCCC 
CGTGAGAGGC GAGGTGGCCT GCGCTTCCCG GAGCGACCCG AGGGGACACC TCCCACCGAG 
AGATACTCCA CGTGCCTCAT GCGCAACGAG AAGGGACTGG GCTTCAGTAT CGCTGGTGGC 
AAAGGCTCCA CTCCTTACCG GGCTGGTGAC ATGGGGATCT TTATCTCCCG GATCGCAGAG 
GGTGGTGCAG CCCATCGGGA CGGGATCCTG CACGTAGGAG ATCGGGTCAT CTCGATCAAT 
GGGGTAGACA TGACAGAAGC CAGGCATGAT CAGGCAGTAG CGCTGCTTAC CGCGTCCTCC 
CCCACCATCG TGCTGCTGGT AGAGAGAGAG GGTGCGGAGC AGCCCGGCGA GGGAGACTGG 
CCCGGGGGGC CACGGGCACG CATGCACTCC CCACCGCCAC CCCCCAGCCA TGGGGAGAGC 
CCGCCCGAGG AGACGCCCGC ACCACAGAGG AACCATCTCT CCAAAGGCCT GGAGGATCAG 
TACCCCATTG AGGAAATTCA CCTGGTGAAA GCAGGTGGTC CCCTGGGGCT CAGCATCGTA 
GGGGGCAGTG ACCACTCAAG CCATCCGTTT GGGATTCATG AACCAGGTGT CTTCATTTCG 
AAGGTCATTC CCCGTGGGCT GGCATCCCGC AGTGGGCTGC GAGTAGGGGA CAGGATCCTG 
GAAGTCAATA GCATTGACCT GCGCCACGCT ACCCACCAGG AAGCGGTGAA CGCCCTGCTC 
TCCAACACCC AGGAGCTGAC CATGTTAGTG AGGCGAGACC CACCACCACC TGGTATGCAG 
GAAATATGCA TTGAGAAGGC TCCAGGAGAA AAGCTGGGCA TCAGCATCCG GGGTGGAGCG 
AAAGGCCATG CTGGAAATCC GTTTGATCCC ACTGACGAGG GCATCTTCAT CTCTAAGGTG 
AGCTCCTCGG GGGCGGCAGC CCGGGACGGC CGCCTGAAGG TGGGGATGCG GATCCTAGAG 
GTGAACCACC AGAGCCTGCT GGGGATGACG CACACGGAAG CGGTGCAGAT GCTGCGCGGG 
GTGGGCGACG CGCTCCTGGT GCTGGTGTGT GACGGCTTCG ACCCCAAGGC TGCAGCTGCC 
ATCGAGATGT CCCCGGGAAT TATTGCAAAC CCTTTCGCTG CTGGTATCGG ACGCAAGAAT 
AGCCTGGAAA GTATCTCTTC CATCGACCGG GATTTAAGCC CTGAGGAACT GGAGATCCTG 
CAGAAGGCTC CTGCAGGCGA GTCTGGGATG GGCAATTCCA GGCGTGAAAC CCCCTATTCT 
CCTGGTAACC AGCAGCGGCC TGCAACCCCT CGGCCCGCTC ACCTGCCGGG CAGGGAGACC 
CGCTTTGCTT CCATTAACTT CATTACATCG CAGGATGACA GTAAATCGAC CAAGCCAGGC 
GTCATTCAGC CTCTTTCCCG AGTGCGACAA AATGCTGCCC CGCACAATTC GGAGGAGGGG 
GACAGCCCCA ATCCCTTCCA GCAGCCCGAG CCGTGCTTCA GTGTCCAGAA CTCTCCAAAG 
CCACCCTCAC CACCCTCTCC TGATGAGGTC CCCATCAATG TCAAGCAAGC GTACAAGACC 
TTCGCTGCAG TGCCCTTGTC ACACCCCCTG CTTGGGACAC AGGAATTGCC TGGTCAGAGC 
GGCACAGAGA ATCCCAGAAG TGCCCCGGAG GTGGCTGCGC ACAGCCCTGG GGGACTGCAC 
AGCCCTGAGC AGAAGTCCTT CCGTGAGAGG CAGAAGTATT TCGAGATTGA GGTGAAGCAG 
CAGCAGATGG ACAAGCCCCC CAAGAGAGTC TCCTTGGTGG GAGAGGACGA CCTGAAGAAG 
ATGAAAGAGG AGGAAGCTCG AAAACTGCAG CAGAAACGTA CCCTTCTGCT GGAGGAAGAG 
GCAGAAGAGG AGGAGATGGC GAAGCAGGCG CCTGAGGTGA GCGTGCAGTC CAGTGTCATC 
ATTGAAGGAG TCGAGTACAA GATTGAGAGA CTCAACGGGA GGAGCAGCCA GTCTCCGGCC 
ACTCGGCCCT CCGAGGTCAA CGAGAACAGC AGCTCGCAGA GGAATTCACT GGAAGAGGGG 
ATCAAGGCTG AGCAGAGAAC CAACTCCATG TCTGGGCTGA GTCCCTTGTA CCTTGGAGCA 
GGGGACGCAG TGGCACCCGT GCGGACGGCC AAAGCTGAAC GGCGACACCA GGAGCGGTTG 
CGAATGCAGA GTCCTGAGCT GCTGAGTGGC CAAGAGAAGG AGCTTTCTCC TGCAGAACGT 
CGTGCTCTGG AGGCGGAGAA ACGAGCAATG TGGAGGGCAG CACGATCCTC AGCTCTTGAA 
GACAGTATTA AACAGTACGA GCAGGACCTG GCCAGGAGGC TGTACCAGTC CCGACAGCGG 
GCACCTGCTC CCGGGGCCGG GCCAGCTCCG ATGCCTAGTC CCAAGCAGAG TCACGCCTCG 
GCACCCCCTG GGTCAGGAAG CCATTCCAGG ATGAAATCGC TTGAACAGGA TGCTCTGAAA 
GCCCAGATGG TCATTGCCAA GTCCAAGGAG GGGAAGAAGC GCAGCCCGCT GGAGCAGCTG 
GCAGAGTCCC CCTCGCCAGT TCCTACACCG TCACCGACAC CACTGGAAGA TTGCAGTCCC 
AGGAACGTCA CCTCCCCAGG AAGGCTGTCC ATGTCTGAAA AGAAGTTTGA CTACCGGGAA 
TTTGCTGCTA TTCCTTCCTC CAAACCCGTT TACGAAATTC AGTCGCCTGA TGCTGCTGAT 
GACCTCAGAT ACATCGAGGA CAGGAATAAC TCAGTTCCCC AGGAGCAGGA TGGGCAGCCC 
GAGGAAGAGG ACACATTAGG AACTCACGAC TCATCCACAC CCACTCCATC AGCACTTGAA 
GAAATGGCCC TGTACAGCAG CAAACGCAAG CTCCGACACA GCCGGCGCAG CCTGGAGGCT 
GCCGTCCCTA CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014801218.1
CDS	6..5360
Translation	MPPLWRCNRHVEALDRRHCSLQAVPEEIYRYSRSLEELLLDANQLRELPKPFFRLLNLRKLGLSDNEIQRLPPEVANFMQLVELDISRNDIPEIPESIKFCKSLEIADFSGNPLSRLPEGFTQLRSLGHLALNDVSLQSLPSDIGNLANLVTLELRENLLKTLPTSLSFLVKLEQLDLGGNDLEVLPDTLGALPNLRELWLDRNQLSALPPELGNLRRLVCLDVSENKLEQLPNEVSGLVALTDLLLSQNLLECIPDGIGQLKQLSILKVDQNRLTEVTESIGDCENLSELILTENMLTALPKSLGKLAKLTNLNVDRNRLTSLPAEIGGCANLNVLSLRDNRLTLLPSELANTTELHVLDVAGNRLQNLPFALTNLNLKALWLAENQSQPMLKFQTEDDEKTGEKVLTCYLLPQQPSPSLENLLQNSVDESWTDTNLNRVSVIQFLDEPKADDEEESGAERRGLQRRATPHPSELKVMKKVIEVRRSEAHAAKPNADLNSPHSEEKRLSATSNRSEDSHLSNSTASADCRAEEQQEEAERSRPNGQVPEMQELEKEAKSRVDEEHKEAAGHEEEDVEVEYNEPTVHFAEDTLIRSEDEDEDEERPFPVEKQRLIRKDTPHYKKHFKITKLPKPEAVVALLQGLNSEGVPKEEEELGGCNNNTEPADQEEVWDEEDRKNGALSAQPSVKGVSFDQANNLLIEPARIEEEELTLTIVRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVRVGDKLLEVNGVSLHSAEHHVAVEALRGSGSSVSMTVLRERMVEPENAITVTPLRPEDDYSPRERRGGLRFPERPEGTPPTERYSTCLMRNEKGLGFSIAGGKGSTPYRAGDMGIFISRIAEGGAAHRDGILHVGDRVISINGVDMTEARHDQAVALLTASSPTIVLLVEREGAEQPGEGDWPGGPRARMHSPPPPPSHGESPPEETPAPQRNHLSKGLEDQYPIEEIHLVKAGGPLGLSIVGGSDHSSHPFGIHEPGVFISKVIPRGLASRSGLRVGDRILEVNSIDLRHATHQEAVNALLSNTQELTMLVRRDPPPPGMQEICIEKAPGEKLGISIRGGAKGHAGNPFDPTDEGIFISKVSSSGAAARDGRLKVGMRILEVNHQSLLGMTHTEAVQMLRGVGDALLVLVCDGFDPKAAAAIEMSPGIIANPFAAGIGRKNSLESISSIDRDLSPEELEILQKAPAGESGMGNSRRETPYSPGNQQRPATPRPAHLPGRETRFASINFITSQDDSKSTKPGVIQPLSRVRQNAAPHNSEEGDSPNPFQQPEPCFSVQNSPKPPSPPSPDEVPINVKQAYKTFAAVPLSHPLLGTQELPGQSGTENPRSAPEVAAHSPGGLHSPEQKSFRERQKYFEIEVKQQQMDKPPKRVSLVGEDDLKKMKEEEARKLQQKRTLLLEEEAEEEEMAKQAPEVSVQSSVIIEGVEYKIERLNGRSSQSPATRPSEVNENSSSQRNSLEEGIKAEQRTNSMSGLSPLYLGAGDAVAPVRTAKAERRHQERLRMQSPELLSGQEKELSPAERRALEAEKRAMWRAARSSALEDSIKQYEQDLARRLYQSRQRAPAPGAGPAPMPSPKQSHASAPPGSGSHSRMKSLEQDALKAQMVIAKSKEGKKRSPLEQLAESPSPVPTPSPTPLEDCSPRNVTSPGRLSMSEKKFDYREFAAIPSSKPVYEIQSPDAADDLRYIEDRNNSVPQEQDGQPEEEDTLGTHDSSTPTPSALEEMALYSSKRKLRHSRRSLEAAVPT

Target ORF information:

RefSeq Version	XM_014945732.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax scribbled planar cell polarity protein (SCRIB), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014945732.1

ORF Insert Sequence:

ATGCCGCCGC TCTGGCGCTG CAACCGGCAC GTGGAGGCGC TGGACCGGCG GCACTGCTCG 
CTGCAGGCCG TGCCCGAGGA GATCTACCGC TACAGCCGCT CGCTGGAGGA GCTGCTGCTG 
GACGCCAACC AGCTGCGGGA GCTGCCCAAG CCTTTCTTCA GGCTTCTGAA CCTGCGCAAG 
CTGGGCCTGA GTGACAATGA GATCCAAAGA CTTCCCCCTG AGGTGGCCAA CTTCATGCAG 
CTGGTGGAAC TGGACATCTC CCGTAACGAC ATTCCAGAAA TTCCTGAAAG CATCAAATTC 
TGCAAATCCT TGGAAATCGC AGACTTCAGT GGGAATCCTC TCTCCAGGCT TCCCGAGGGC 
TTCACGCAGC TTCGGAGCCT GGGCCATTTG GCCCTGAATG ATGTGTCCCT GCAGAGCCTC 
CCCAGTGACA TTGGGAATTT GGCAAACCTG GTGACTCTGG AGCTACGCGA GAACCTGCTA 
AAGACTCTCC CCACCTCGCT GTCCTTCCTT GTCAAGTTAG AGCAGCTGGA TCTCGGTGGC 
AATGACCTGG AGGTTCTGCC AGACACTCTG GGAGCGCTCC CCAACCTGCG TGAGCTCTGG 
TTAGACCGGA ACCAGCTCTC AGCCCTGCCT CCCGAGCTGG GCAACCTCCG CCGCCTGGTC 
TGCCTGGATG TGTCGGAGAA CAAGCTGGAG CAGTTGCCCA ACGAGGTCAG CGGCCTGGTG 
GCACTGACCG ACTTGCTGCT GTCGCAGAAC CTGTTGGAGT GCATTCCTGA TGGGATTGGT 
CAGCTGAAGC AGCTCTCCAT CCTCAAGGTG GACCAGAATC GACTGACAGA GGTGACAGAG 
TCCATTGGGG ACTGTGAAAA TCTGTCCGAG CTCATCCTGA CAGAGAACAT GCTGACGGCC 
TTGCCGAAAT CCCTTGGGAA GCTGGCCAAG CTGACCAACC TGAACGTGGA TCGGAACCGG 
CTGACATCCC TCCCGGCCGA GATCGGTGGC TGTGCCAACC TCAACGTGCT GTCCTTGCGG 
GACAATCGCC TGACCCTCCT GCCATCGGAG CTGGCCAACA CCACCGAGCT TCATGTTCTG 
GATGTGGCTG GGAACAGGCT GCAGAACTTG CCGTTTGCTC TGACAAACCT CAACCTGAAA 
GCCCTGTGGC TGGCGGAGAA TCAGTCCCAG CCCATGCTGA AATTCCAAAC AGAGGATGAT 
GAGAAGACAG GAGAGAAAGT TCTCACTTGT TACCTGCTTC CTCAGCAGCC CTCTCCCAGC 
CTGGAGAACC TTCTACAGAA CAGCGTGGAC GAGAGCTGGA CGGACACCAA TTTAAACAGA 
GTCAGTGTGA TTCAGTTCCT GGATGAACCC AAAGCAGATG ATGAAGAAGA GTCTGGAGCT 
GAGAGAAGGG GCTTGCAGCG CAGAGCAACC CCCCATCCCA GCGAGCTGAA GGTGATGAAG 
AAGGTGATTG AAGTTCGGCG CAGTGAGGCC CACGCTGCAA AGCCCAATGC AGACCTGAAT 
TCTCCTCACT CAGAAGAGAA GAGGCTGAGC GCCACGTCCA ACCGCAGCGA GGACTCCCAC 
CTTTCCAACA GCACTGCCTC TGCTGACTGT AGGGCGGAGG AGCAGCAGGA GGAGGCAGAG 
AGGAGTCGGC CAAACGGGCA AGTGCCTGAG ATGCAGGAGC TGGAGAAGGA GGCAAAGTCC 
AGAGTGGACG AGGAGCACAA GGAAGCTGCT GGCCATGAAG AGGAAGATGT GGAAGTGGAA 
TACAATGAGC CCACTGTACA CTTTGCTGAA GACACGCTAA TACGGAGTGA GGATGAAGAT 
GAAGATGAAG AGCGACCGTT CCCAGTGGAG AAGCAGCGGC TCATCCGCAA GGACACCCCT 
CACTATAAGA AACACTTTAA GATAACTAAA TTGCCCAAGC CAGAGGCGGT GGTGGCCCTG 
CTGCAGGGGC TGAACAGCGA GGGGGTCCCC AAGGAGGAAG AGGAGTTGGG AGGCTGCAAC 
AACAACACAG AGCCTGCAGA CCAGGAGGAG GTGTGGGATG AGGAGGACCG GAAGAATGGA 
GCTTTGTCTG CCCAGCCATC GGTGAAGGGG GTGTCGTTTG ATCAAGCCAA TAATCTGCTG 
ATTGAACCTG CTCGCATTGA GGAGGAAGAG TTGACTCTGA CTATCGTACG GCAGACGGGG 
GGGCTGGGCA TCAGCATCGC AGGGGGCAAG GGCTCCACCC CCTATAAGGG CGATGATGAG 
GGCATCTTTA TTTCTCGGGT GTCAGAAGAA GGTCCTGCGG CTCGTGCAGG GGTTCGTGTT 
GGGGACAAGC TCCTGGAGGT GAACGGTGTG TCCCTGCACT CCGCTGAGCA CCACGTGGCA 
GTGGAGGCTC TGCGTGGATC GGGAAGTTCC GTCTCCATGA CTGTTCTGCG GGAGCGGATG 
GTGGAGCCGG AGAATGCCAT CACTGTCACC CCGCTGCGCC CCGAGGATGA CTACAGCCCC 
CGTGAGAGGC GAGGTGGCCT GCGCTTCCCG GAGCGACCCG AGGGGACACC TCCCACCGAG 
AGATACTCCA CGTGCCTCAT GCGCAACGAG AAGGGACTGG GCTTCAGTAT CGCTGGTGGC 
AAAGGCTCCA CTCCTTACCG GGCTGGTGAC ATGGGGATCT TTATCTCCCG GATCGCAGAG 
GGTGGTGCAG CCCATCGGGA CGGGATCCTG CACGTAGGAG ATCGGGTCAT CTCGATCAAT 
GGGGTAGACA TGACAGAAGC CAGGCATGAT CAGGCAGTAG CGCTGCTTAC CGCGTCCTCC 
CCCACCATCG TGCTGCTGGT AGAGAGAGAG GGTGCGGAGC AGCCCGGCGA GGGAGACTGG 
CCCGGGGGGC CACGGGCACG CATGCACTCC CCACCGCCAC CCCCCAGCCA TGGGGAGAGC 
CCGCCCGAGG AGACGCCCGC ACCACAGAGG AACCATCTCT CCAAAGGCCT GGAGGATCAG 
TACCCCATTG AGGAAATTCA CCTGGTGAAA GCAGGTGGTC CCCTGGGGCT CAGCATCGTA 
GGGGGCAGTG ACCACTCAAG CCATCCGTTT GGGATTCATG AACCAGGTGT CTTCATTTCG 
AAGGTCATTC CCCGTGGGCT GGCATCCCGC AGTGGGCTGC GAGTAGGGGA CAGGATCCTG 
GAAGTCAATA GCATTGACCT GCGCCACGCT ACCCACCAGG AAGCGGTGAA CGCCCTGCTC 
TCCAACACCC AGGAGCTGAC CATGTTAGTG AGGCGAGACC CACCACCACC TGGTATGCAG 
GAAATATGCA TTGAGAAGGC TCCAGGAGAA AAGCTGGGCA TCAGCATCCG GGGTGGAGCG 
AAAGGCCATG CTGGAAATCC GTTTGATCCC ACTGACGAGG GCATCTTCAT CTCTAAGGTG 
AGCTCCTCGG GGGCGGCAGC CCGGGACGGC CGCCTGAAGG TGGGGATGCG GATCCTAGAG 
GTGAACCACC AGAGCCTGCT GGGGATGACG CACACGGAAG CGGTGCAGAT GCTGCGCGGG 
GTGGGCGACG CGCTCCTGGT GCTGGTGTGT GACGGCTTCG ACCCCAAGGC TGCAGCTGCC 
ATCGAGATGT CCCCGGGAAT TATTGCAAAC CCTTTCGCTG CTGGTATCGG ACGCAAGAAT 
AGCCTGGAAA GTATCTCTTC CATCGACCGG GATTTAAGCC CTGAGGAACT GGAGATCCTG 
CAGAAGGCTC CTGCAGGCGA GTCTGGGATG GGCAATTCCA GGCGTGAAAC CCCCTATTCT 
CCTGGTAACC AGCAGCGGCC TGCAACCCCT CGGCCCGCTC ACCTGCCGGG CAGGGAGACC 
CGCTTTGCTT CCATTAACTT CATTACATCG CAGGATGACA GTAAATCGAC CAAGCCAGGC 
GTCATTCAGC CTCTTTCCCG AGTGCGACAA AATGCTGCCC CGCACAATTC GGAGGAGGGG 
GACAGCCCCA ATCCCTTCCA GCAGCCCGAG CCGTGCTTCA GTGTCCAGAA CTCTCCAAAG 
CCACCCTCAC CACCCTCTCC TGATGAGGTC CCCATCAATG TCAAGCAAGC GTACAAGACC 
TTCGCTGCAG TGCCCTTGTC ACACCCCCTG CTTGGGACAC AGGAATTGCC TGGTCAGAGC 
GGCACAGAGA ATCCCAGAAG TGCCCCGGAG GTGGCTGCGC ACAGCCCTGG GGGACTGCAC 
AGCCCTGAGC AGAAGTCCTT CCGTGAGAGG CAGAAGTATT TCGAGATTGA GGTGAAGCAG 
CAGCAGATGG ACAAGCCCCC CAAGAGAGTC TCCTTGGTGG GAGAGGACGA CCTGAAGAAG 
ATGAAAGAGG AGGAAGCTCG AAAACTGCAG CAGAAACGTA CCCTTCTGCT GGAGGAAGAG 
GCAGAAGAGG AGGAGATGGC GAAGCAGGCG CCTGAGGTGA GCGTGCAGTC CAGTGTCATC 
ATTGAAGGAG TCGAGTACAA GATTGAGAGA CTCAACGGGA GGAGCAGCCA GTCTCCGGCC 
ACTCGGCCCT CCGAGGTCAA CGAGAACAGC AGCTCGCAGA GGAATTCACT GGAAGAGGGG 
ATCAAGGCTG AGCAGAGAAC CAACTCCATG TCTGGGCTGA GTCCCTTGTA CCTTGGAGCA 
GGGGACGCAG TGGCACCCGT GCGGACGGCC AAAGCTGAAC GGCGACACCA GGAGCGGTTG 
CGAATGCAGA GTCCTGAGCT GCTGAGTGGC CAAGAGAAGG AGCTTTCTCC TGCAGAACGT 
CGTGCTCTGG AGGCGGAGAA ACGAGCAATG TGGAGGGCAG CACGATCCTC AGCTCTTGAA 
GACAGTATTA AACAGTACGA GCAGGACCTG GCCAGGAGGC TGTACCAGTC CCGACAGCGG 
GCACCTGCTC CCGGGGCCGG GCCAGCTCCG ATGCCTAGTC CCAAGCAGAG TCACGCCTCG 
GCACCCCCTG GGTCAGGAAG CCATTCCAGG ATGAAATCGC TTGAACAGGA TGCTCTGAAA 
GCCCAGATGG TCATTGCCAA GTCCAAGGAG GGGAAGAAGC GCAGCCCGCT GGAGCAGCTG 
GCAGAGTCCC CCTCGCCAGT TCCTACACCG TCACCGACAC CACTGGAAGA TTGCAGTCCC 
AGGAACGTCA CCTCCCCAGG AAGGCTGTCC ATGTCTGAAA AGAAGTTTGA CTACCGGGAA 
TTTGCTGCTA TTCCTTCCTC CAAACCCGTT TACGAAATTC AGTCGCCTGA TGCTGCTGAT 
GACCTCAGAT ACATCGAGGA CAGGAATAAC TCAGTTCCCC AGGAGCAGGA TGGGCAGCCC 
GAGGAAGAGG ACACATTAGG AACTCACGAC TCATCCACAC CCACTCCATC AGCACTTGAA 
GAAATGGCCC TGTACAGCAG CAAACGCAAG CTCCGACACA GCCGGCGCAG CCTGGAGGCT 
GCCGTCCCTA CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCRIB ( XM_014945733.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014801219.1 Calidris pugnax scribbled planar cell polarity protein (SCRIB), transcript variant X19, mRNA.

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CloneID	OCa67765
Clone ID Related Accession (Same CDS sequence)	XM_014945733.1
Accession Version	XM_014945733.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	protein scribble homolog isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090871.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCCGC TCTGGCGCTG CAACCGGCAC GTGGAGGCGC TGGACCGGCG GCACTGCTCG 
CTGCAGGCCG TGCCCGAGGA GATCTACCGC TACAGCCGCT CGCTGGAGGA GCTGCTGCTG 
GACGCCAACC AGCTGCGGGA GCTGCCCAAG CCTTTCTTCA GGCTTCTGAA CCTGCGCAAG 
CTGGGCCTGA GTGACAATGA GATCCAAAGA CTTCCCCCTG AGGTGGCCAA CTTCATGCAG 
CTGGTGGAAC TGGACATCTC CCGTAACGAC ATTCCAGAAA TTCCTGAAAG CATCAAATTC 
TGCAAATCCT TGGAAATCGC AGACTTCAGT GGGAATCCTC TCTCCAGGCT TCCCGAGGGC 
TTCACGCAGC TTCGGAGCCT GGGCCATTTG GCCCTGAATG ATGTGTCCCT GCAGAGCCTC 
CCCAGTGACA TTGGGAATTT GGCAAACCTG GTGACTCTGG AGCTACGCGA GAACCTGCTA 
AAGACTCTCC CCACCTCGCT GTCCTTCCTT GTCAAGTTAG AGCAGCTGGA TCTCGGTGGC 
AATGACCTGG AGGTTCTGCC AGACACTCTG GGAGCGCTCC CCAACCTGCG TGAGCTCTGG 
TTAGACCGGA ACCAGCTCTC AGCCCTGCCT CCCGAGCTGG GCAACCTCCG CCGCCTGGTC 
TGCCTGGATG TGTCGGAGAA CAAGCTGGAG CAGTTGCCCA ACGAGGTCAG CGGCCTGGTG 
GCACTGACCG ACTTGCTGCT GTCGCAGAAC CTGTTGGAGT GCATTCCTGA TGGGATTGGT 
CAGCTGAAGC AGCTCTCCAT CCTCAAGGTG GACCAGAATC GACTGACAGA GGTGACAGAG 
TCCATTGGGG ACTGTGAAAA TCTGTCCGAG CTCATCCTGA CAGAGAACAT GCTGACGGCC 
TTGCCGAAAT CCCTTGGGAA GCTGGCCAAG CTGACCAACC TGAACGTGGA TCGGAACCGG 
CTGACATCCC TCCCGGCCGA GATCGGTGGC TGTGCCAACC TCAACGTGCT GTCCTTGCGG 
GACAATCGCC TGACCCTCCT GCCATCGGAG CTGGCCAACA CCACCGAGCT TCATGTTCTG 
GATGTGGCTG GGAACAGGCT GCAGAACTTG CCGTTTGCTC TGACAAACCT CAACCTGAAA 
GCCCTGTGGC TGGCGGAGAA TCAGTCCCAG CCCATGCTGA AATTCCAAAC AGAGGATGAT 
GAGAAGACAG GAGAGAAAGT TCTCACTTGT TACCTGCTTC CTCAGCAGCC CTCTCCCAGC 
CTGGAGAACC TTCTACAGAA CAGCGTGGAC GAGAGCTGGA CGGACACCAA TTTAAACAGA 
GTCAGTGTGA TTCAGTTCCT GGATGAACCC AAAGCAGATG ATGAAGAAGA GTCTGGAGCT 
GAGAGAAGGG GCTTGCAGCG CAGAGCAACC CCCCATCCCA GCGAGCTGAA GGTGATGAAG 
AAGGTGATTG AAGTTCGGCG CAGTGAGGCC CACGCTGCAA AGCCCAATGC AGACCTGAAT 
TCTCCTCACT CAGAAGAGAA GAGGCTGAGC GCCACGTCCA ACCGCAGCGA GGACTCCCAC 
CTTTCCAACA GCACTGCCTC TGCTGACTGT AGGGCGGAGG AGCAGCAGGA GGAGGCAGAG 
AGGAGTCGGC CAAACGGGCA AGTGCCTGAG ATGCAGGAGC TGGAGAAGGA GGCAAAGTCC 
AGAGTGGACG AGGAGCACAA GGAAGCTGCT GGCCATGAAG AGGAAGATGT GGAAGTGGAA 
TACAATGAGC CCACTGTACA CTTTGCTGAA GACACGCTAA TACGGAGTGA GGATGAAGAT 
GAAGATGAAG AGCGACCGTT CCCAGTGGAG AAGCAGCGGC TCATCCGCAA GGACACCCCT 
CACTATAAGA AACACTTTAA GATAACTAAA TTGCCCAAGC CAGAGGCGGT GGTGGCCCTG 
CTGCAGGGGC TGAACAGCGA GGGGGTCCCC AAGGAGGAAG AGGAGTTGGG AGGCTGCAAC 
AACAACACAG AGCCTGCAGA CCAGGAGGAG GTGTGGGATG AGGAGGACCG GAAGAATGGA 
GCTTTGTCTG CCCAGCCATC GGTGAAGGGG GTGTCGTTTG ATCAAGCCAA TAATCTGCTG 
ATTGAACCTG CTCGCATTGA GGAGGAAGAG TTGACTCTGA CTATCGTACG GCAGACGGGG 
GGGCTGGGCA TCAGCATCGC AGGGGGCAAG GGCTCCACCC CCTATAAGGG CGATGATGAG 
GGCATCTTTA TTTCTCGGGT GTCAGAAGAA GGTCCTGCGG CTCGTGCAGG GGTTCGTGTT 
GGGGACAAGC TCCTGGAGGT GAACGGTGTG TCCCTGCACT CCGCTGAGCA CCACGTGGCA 
GTGGAGGCTC TGCGTGGATC GGGAAGTTCC GTCTCCATGA CTGTTCTGCG GGAGCGGATG 
GTGGAGCCGG AGAATGCCAT CACTGTCACC CCGCTGCGCC CCGAGGATGA CTACAGCCCC 
CGTGAGAGGC GAGGTGGCCT GCGCTTCCCG GAGCGACCCG AGGGGACACC TCCCACCGAG 
AGATACTCCA CGTGCCTCAT GCGCAACGAG AAGGGACTGG GCTTCAGTAT CGCTGGTGGC 
AAAGGCTCCA CTCCTTACCG GGCTGGTGAC ATGGGGATCT TTATCTCCCG GATCGCAGAG 
GGTGGTGCAG CCCATCGGGA CGGGATCCTG CACGTAGGAG ATCGGGTCAT CTCGATCAAT 
GGGGTAGACA TGACAGAAGC CAGGCATGAT CAGGCAGTAG CGCTGCTTAC CGCGTCCTCC 
CCCACCATCG TGCTGCTGGT AGAGAGAGAG GGTGCGGAGC AGCCCGGCGA GGGAGACTGG 
CCCGGGGGGC CACGGGCACG CATGCACTCC CCACCGCCAC CCCCCAGCCA TGGGGAGAGC 
CCGCCCGAGG AGACGCCCGC ACCACAGAGG AACCATCTCT CCAAAGGCCT GGAGGATCAG 
TACCCCATTG AGGAAATTCA CCTGGTGAAA GCAGGTGGTC CCCTGGGGCT CAGCATCGTA 
GGGGGCAGTG ACCACTCAAG CCATCCGTTT GGGATTCATG AACCAGGTGT CTTCATTTCG 
AAGGTCATTC CCCGTGGGCT GGCATCCCGC AGTGGGCTGC GAGTAGGGGA CAGGATCCTG 
GAAGTCAATA GCATTGACCT GCGCCACGCT ACCCACCAGG AAGCGGTGAA CGCCCTGCTC 
TCCAACACCC AGGAGCTGAC CATGTTAGTG AGGCGAGACC CACCACCACC TGGTATGCAG 
GAAATATGCA TTGAGAAGGC TCCAGGAGAA AAGCTGGGCA TCAGCATCCG GGGTGGAGCG 
AAAGGCCATG CTGGAAATCC GTTTGATCCC ACTGACGAGG GCATCTTCAT CTCTAAGGTG 
AGCTCCTCGG GGGCGGCAGC CCGGGACGGC CGCCTGAAGG TGGGGATGCG GATCCTAGAG 
GTGAACCACC AGAGCCTGCT GGGGATGACG CACACGGAAG CGGTGCAGAT GCTGCGCGGG 
GTGGGCGACG CGCTCCTGGT GCTGGTGTGT GACGGCTTCG ACCCCAAGGC TGCAGCTGCC 
ATCGAGATGT CCCCGGGAAT TATTGCAAAC CCTTTCGCTG CTGGTATCGG ACGCAAGAAT 
AGCCTGGAAA GTATCTCTTC CATCGACCGG GATTTAAGCC CTGAGGAACT GGAGATCCTG 
CAGAAGGCTC CTGCAGGCGA GTCTGGGATG GGCAATTCCA GGCGTGAAAC CCCCTATTCT 
CCTGGTAACC AGCAGGCTTC CATTAACTTC ATTACATCGC AGGATGACAG TAAATCGACC 
AAGCCAGGCG TCATTCAGCC TCTTTCCCGA GTGCGACAAA ATGCTGCCCC GCACAATTCG 
GAGGAGGGGG ACAGCCCCAA TCCCTTCCAG CAGCCCGAGC CGTGCTTCAG TGTCCAGAAC 
TCTCCAAAGC CACCCTCACC ACCCTCTCCT GATGAGGTCC CCATCAATGT CAAGCAAGCG 
TACAAGACCT TCGCTGCAGT GCCCTTGTCA CACCCCCTGC TTGGGACACA GGAATTGCCT 
GGTCAGAGCG GCACAGAGAA TCCCAGAAGT GCCCCGGAGG TGGCTGCGCA CAGCCCTGGG 
GGACTGCACA GCCCTGAGCA GAAGTCCTTC CGTGAGAGGC AGAAGTATTT CGAGATTGAG 
GTGAAGCAGC AGCAGATGGA CAAGCCCCCC AAGAGAGTCT CCTTGGTGGG AGAGGACGAC 
CTGAAGAAGA TGAAAGAGGA GGAAGCTCGA AAACTGCAGC AGAAACGTAC CCTTCTGCTG 
GAGGAAGAGG CAGAAGAGGA GGAGATGGCG AAGCAGGCGC CTGAGGTGAG CGTGCAGTCC 
AGTGTCATCA TTGAAGGAGT CGAGTACAAG ATTGAGAGAC TCAACGGGAG GAGCAGCCAG 
TCTCCGGCCA CTCGGCCCTC CGAGGTCAAC GAGAACAGCA GCTCGCAGAG GAATTCACTG 
GAAGAGGGGA TCAAGGCTGA GCAGAGAACC AACTCCATGT CTGGGCTGAG TCCCTTGTAC 
CTTGGAGCAG GGGACGCAGT GGCACCCGTG CGGACGGCCA AAGCTGAACG GCGACACCAG 
GAGCGGTTGC GAATGCAGAG TCCTGAGCTG CTGAGTGGCC AAGAGAAGGA GCTTTCTCCT 
GCAGAACGTC GTGCTCTGGA GGCGGAGAAA CGAGCAATGT GGAGGGCAGC ACGATCCTCA 
GCTCTTGAAG ACAGTATTAA ACAGTACGAG CAGGACCTGG CCAGGAGGCT GTACCAGTCC 
CGACAGCGGG CACCTGCTCC CGGGGCCGGG CCAGCTCCGA TGCCTAGTCC CAAGCAGAGT 
CACGCCTCGG CACCCCCTGG GTCAGGAAGC CATTCCAGGA TGAAATCGCT TGAACAGGAT 
GCTCTGAAAG CCCAGATGGT CATTGCCAAG TCCAAGGAGG GGAAGAAGCG CAGCCCGCTG 
GAGCAGCTGG CAGAGTCCCC CTCGCCAGTT CCTACACCGT CACCGACACC ACTGGAAGAT 
TGCAGTCCCA GGAACGTCAC CTCCCCAGGA AGGCTGTCCA TGTCTGAAAA GAAGTTTGAC 
TACCGGGAAT TTGCTGCTAT TCCTTCCTCC AAACCCGTTT ACGAAATTCA GTCGCCTGAT 
GCTGCTGATG ACCTCAGATA CATCGAGGAC AGGAATAACT CAGTTCCCCA GGAGCAGGAT 
GGGCAGCCCG AGGAAGAGGA CACATTAGGA ACTCACGACT CATCCACACC CACTCCATCA 
GCACTTGAAG AAATGGCCCT GTACAGCAGC AAACGCAAGC TCCGACACAG CCGGCGCAGC 
CTGGAGGCTG CCGTCCCTAC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014801219.1
CDS	6..5309
Translation	MPPLWRCNRHVEALDRRHCSLQAVPEEIYRYSRSLEELLLDANQLRELPKPFFRLLNLRKLGLSDNEIQRLPPEVANFMQLVELDISRNDIPEIPESIKFCKSLEIADFSGNPLSRLPEGFTQLRSLGHLALNDVSLQSLPSDIGNLANLVTLELRENLLKTLPTSLSFLVKLEQLDLGGNDLEVLPDTLGALPNLRELWLDRNQLSALPPELGNLRRLVCLDVSENKLEQLPNEVSGLVALTDLLLSQNLLECIPDGIGQLKQLSILKVDQNRLTEVTESIGDCENLSELILTENMLTALPKSLGKLAKLTNLNVDRNRLTSLPAEIGGCANLNVLSLRDNRLTLLPSELANTTELHVLDVAGNRLQNLPFALTNLNLKALWLAENQSQPMLKFQTEDDEKTGEKVLTCYLLPQQPSPSLENLLQNSVDESWTDTNLNRVSVIQFLDEPKADDEEESGAERRGLQRRATPHPSELKVMKKVIEVRRSEAHAAKPNADLNSPHSEEKRLSATSNRSEDSHLSNSTASADCRAEEQQEEAERSRPNGQVPEMQELEKEAKSRVDEEHKEAAGHEEEDVEVEYNEPTVHFAEDTLIRSEDEDEDEERPFPVEKQRLIRKDTPHYKKHFKITKLPKPEAVVALLQGLNSEGVPKEEEELGGCNNNTEPADQEEVWDEEDRKNGALSAQPSVKGVSFDQANNLLIEPARIEEEELTLTIVRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVRVGDKLLEVNGVSLHSAEHHVAVEALRGSGSSVSMTVLRERMVEPENAITVTPLRPEDDYSPRERRGGLRFPERPEGTPPTERYSTCLMRNEKGLGFSIAGGKGSTPYRAGDMGIFISRIAEGGAAHRDGILHVGDRVISINGVDMTEARHDQAVALLTASSPTIVLLVEREGAEQPGEGDWPGGPRARMHSPPPPPSHGESPPEETPAPQRNHLSKGLEDQYPIEEIHLVKAGGPLGLSIVGGSDHSSHPFGIHEPGVFISKVIPRGLASRSGLRVGDRILEVNSIDLRHATHQEAVNALLSNTQELTMLVRRDPPPPGMQEICIEKAPGEKLGISIRGGAKGHAGNPFDPTDEGIFISKVSSSGAAARDGRLKVGMRILEVNHQSLLGMTHTEAVQMLRGVGDALLVLVCDGFDPKAAAAIEMSPGIIANPFAAGIGRKNSLESISSIDRDLSPEELEILQKAPAGESGMGNSRRETPYSPGNQQASINFITSQDDSKSTKPGVIQPLSRVRQNAAPHNSEEGDSPNPFQQPEPCFSVQNSPKPPSPPSPDEVPINVKQAYKTFAAVPLSHPLLGTQELPGQSGTENPRSAPEVAAHSPGGLHSPEQKSFRERQKYFEIEVKQQQMDKPPKRVSLVGEDDLKKMKEEEARKLQQKRTLLLEEEAEEEEMAKQAPEVSVQSSVIIEGVEYKIERLNGRSSQSPATRPSEVNENSSSQRNSLEEGIKAEQRTNSMSGLSPLYLGAGDAVAPVRTAKAERRHQERLRMQSPELLSGQEKELSPAERRALEAEKRAMWRAARSSALEDSIKQYEQDLARRLYQSRQRAPAPGAGPAPMPSPKQSHASAPPGSGSHSRMKSLEQDALKAQMVIAKSKEGKKRSPLEQLAESPSPVPTPSPTPLEDCSPRNVTSPGRLSMSEKKFDYREFAAIPSSKPVYEIQSPDAADDLRYIEDRNNSVPQEQDGQPEEEDTLGTHDSSTPTPSALEEMALYSSKRKLRHSRRSLEAAVPT

Target ORF information:

RefSeq Version	XM_014945733.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax scribbled planar cell polarity protein (SCRIB), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014945733.1

ORF Insert Sequence:

ATGCCGCCGC TCTGGCGCTG CAACCGGCAC GTGGAGGCGC TGGACCGGCG GCACTGCTCG 
CTGCAGGCCG TGCCCGAGGA GATCTACCGC TACAGCCGCT CGCTGGAGGA GCTGCTGCTG 
GACGCCAACC AGCTGCGGGA GCTGCCCAAG CCTTTCTTCA GGCTTCTGAA CCTGCGCAAG 
CTGGGCCTGA GTGACAATGA GATCCAAAGA CTTCCCCCTG AGGTGGCCAA CTTCATGCAG 
CTGGTGGAAC TGGACATCTC CCGTAACGAC ATTCCAGAAA TTCCTGAAAG CATCAAATTC 
TGCAAATCCT TGGAAATCGC AGACTTCAGT GGGAATCCTC TCTCCAGGCT TCCCGAGGGC 
TTCACGCAGC TTCGGAGCCT GGGCCATTTG GCCCTGAATG ATGTGTCCCT GCAGAGCCTC 
CCCAGTGACA TTGGGAATTT GGCAAACCTG GTGACTCTGG AGCTACGCGA GAACCTGCTA 
AAGACTCTCC CCACCTCGCT GTCCTTCCTT GTCAAGTTAG AGCAGCTGGA TCTCGGTGGC 
AATGACCTGG AGGTTCTGCC AGACACTCTG GGAGCGCTCC CCAACCTGCG TGAGCTCTGG 
TTAGACCGGA ACCAGCTCTC AGCCCTGCCT CCCGAGCTGG GCAACCTCCG CCGCCTGGTC 
TGCCTGGATG TGTCGGAGAA CAAGCTGGAG CAGTTGCCCA ACGAGGTCAG CGGCCTGGTG 
GCACTGACCG ACTTGCTGCT GTCGCAGAAC CTGTTGGAGT GCATTCCTGA TGGGATTGGT 
CAGCTGAAGC AGCTCTCCAT CCTCAAGGTG GACCAGAATC GACTGACAGA GGTGACAGAG 
TCCATTGGGG ACTGTGAAAA TCTGTCCGAG CTCATCCTGA CAGAGAACAT GCTGACGGCC 
TTGCCGAAAT CCCTTGGGAA GCTGGCCAAG CTGACCAACC TGAACGTGGA TCGGAACCGG 
CTGACATCCC TCCCGGCCGA GATCGGTGGC TGTGCCAACC TCAACGTGCT GTCCTTGCGG 
GACAATCGCC TGACCCTCCT GCCATCGGAG CTGGCCAACA CCACCGAGCT TCATGTTCTG 
GATGTGGCTG GGAACAGGCT GCAGAACTTG CCGTTTGCTC TGACAAACCT CAACCTGAAA 
GCCCTGTGGC TGGCGGAGAA TCAGTCCCAG CCCATGCTGA AATTCCAAAC AGAGGATGAT 
GAGAAGACAG GAGAGAAAGT TCTCACTTGT TACCTGCTTC CTCAGCAGCC CTCTCCCAGC 
CTGGAGAACC TTCTACAGAA CAGCGTGGAC GAGAGCTGGA CGGACACCAA TTTAAACAGA 
GTCAGTGTGA TTCAGTTCCT GGATGAACCC AAAGCAGATG ATGAAGAAGA GTCTGGAGCT 
GAGAGAAGGG GCTTGCAGCG CAGAGCAACC CCCCATCCCA GCGAGCTGAA GGTGATGAAG 
AAGGTGATTG AAGTTCGGCG CAGTGAGGCC CACGCTGCAA AGCCCAATGC AGACCTGAAT 
TCTCCTCACT CAGAAGAGAA GAGGCTGAGC GCCACGTCCA ACCGCAGCGA GGACTCCCAC 
CTTTCCAACA GCACTGCCTC TGCTGACTGT AGGGCGGAGG AGCAGCAGGA GGAGGCAGAG 
AGGAGTCGGC CAAACGGGCA AGTGCCTGAG ATGCAGGAGC TGGAGAAGGA GGCAAAGTCC 
AGAGTGGACG AGGAGCACAA GGAAGCTGCT GGCCATGAAG AGGAAGATGT GGAAGTGGAA 
TACAATGAGC CCACTGTACA CTTTGCTGAA GACACGCTAA TACGGAGTGA GGATGAAGAT 
GAAGATGAAG AGCGACCGTT CCCAGTGGAG AAGCAGCGGC TCATCCGCAA GGACACCCCT 
CACTATAAGA AACACTTTAA GATAACTAAA TTGCCCAAGC CAGAGGCGGT GGTGGCCCTG 
CTGCAGGGGC TGAACAGCGA GGGGGTCCCC AAGGAGGAAG AGGAGTTGGG AGGCTGCAAC 
AACAACACAG AGCCTGCAGA CCAGGAGGAG GTGTGGGATG AGGAGGACCG GAAGAATGGA 
GCTTTGTCTG CCCAGCCATC GGTGAAGGGG GTGTCGTTTG ATCAAGCCAA TAATCTGCTG 
ATTGAACCTG CTCGCATTGA GGAGGAAGAG TTGACTCTGA CTATCGTACG GCAGACGGGG 
GGGCTGGGCA TCAGCATCGC AGGGGGCAAG GGCTCCACCC CCTATAAGGG CGATGATGAG 
GGCATCTTTA TTTCTCGGGT GTCAGAAGAA GGTCCTGCGG CTCGTGCAGG GGTTCGTGTT 
GGGGACAAGC TCCTGGAGGT GAACGGTGTG TCCCTGCACT CCGCTGAGCA CCACGTGGCA 
GTGGAGGCTC TGCGTGGATC GGGAAGTTCC GTCTCCATGA CTGTTCTGCG GGAGCGGATG 
GTGGAGCCGG AGAATGCCAT CACTGTCACC CCGCTGCGCC CCGAGGATGA CTACAGCCCC 
CGTGAGAGGC GAGGTGGCCT GCGCTTCCCG GAGCGACCCG AGGGGACACC TCCCACCGAG 
AGATACTCCA CGTGCCTCAT GCGCAACGAG AAGGGACTGG GCTTCAGTAT CGCTGGTGGC 
AAAGGCTCCA CTCCTTACCG GGCTGGTGAC ATGGGGATCT TTATCTCCCG GATCGCAGAG 
GGTGGTGCAG CCCATCGGGA CGGGATCCTG CACGTAGGAG ATCGGGTCAT CTCGATCAAT 
GGGGTAGACA TGACAGAAGC CAGGCATGAT CAGGCAGTAG CGCTGCTTAC CGCGTCCTCC 
CCCACCATCG TGCTGCTGGT AGAGAGAGAG GGTGCGGAGC AGCCCGGCGA GGGAGACTGG 
CCCGGGGGGC CACGGGCACG CATGCACTCC CCACCGCCAC CCCCCAGCCA TGGGGAGAGC 
CCGCCCGAGG AGACGCCCGC ACCACAGAGG AACCATCTCT CCAAAGGCCT GGAGGATCAG 
TACCCCATTG AGGAAATTCA CCTGGTGAAA GCAGGTGGTC CCCTGGGGCT CAGCATCGTA 
GGGGGCAGTG ACCACTCAAG CCATCCGTTT GGGATTCATG AACCAGGTGT CTTCATTTCG 
AAGGTCATTC CCCGTGGGCT GGCATCCCGC AGTGGGCTGC GAGTAGGGGA CAGGATCCTG 
GAAGTCAATA GCATTGACCT GCGCCACGCT ACCCACCAGG AAGCGGTGAA CGCCCTGCTC 
TCCAACACCC AGGAGCTGAC CATGTTAGTG AGGCGAGACC CACCACCACC TGGTATGCAG 
GAAATATGCA TTGAGAAGGC TCCAGGAGAA AAGCTGGGCA TCAGCATCCG GGGTGGAGCG 
AAAGGCCATG CTGGAAATCC GTTTGATCCC ACTGACGAGG GCATCTTCAT CTCTAAGGTG 
AGCTCCTCGG GGGCGGCAGC CCGGGACGGC CGCCTGAAGG TGGGGATGCG GATCCTAGAG 
GTGAACCACC AGAGCCTGCT GGGGATGACG CACACGGAAG CGGTGCAGAT GCTGCGCGGG 
GTGGGCGACG CGCTCCTGGT GCTGGTGTGT GACGGCTTCG ACCCCAAGGC TGCAGCTGCC 
ATCGAGATGT CCCCGGGAAT TATTGCAAAC CCTTTCGCTG CTGGTATCGG ACGCAAGAAT 
AGCCTGGAAA GTATCTCTTC CATCGACCGG GATTTAAGCC CTGAGGAACT GGAGATCCTG 
CAGAAGGCTC CTGCAGGCGA GTCTGGGATG GGCAATTCCA GGCGTGAAAC CCCCTATTCT 
CCTGGTAACC AGCAGGCTTC CATTAACTTC ATTACATCGC AGGATGACAG TAAATCGACC 
AAGCCAGGCG TCATTCAGCC TCTTTCCCGA GTGCGACAAA ATGCTGCCCC GCACAATTCG 
GAGGAGGGGG ACAGCCCCAA TCCCTTCCAG CAGCCCGAGC CGTGCTTCAG TGTCCAGAAC 
TCTCCAAAGC CACCCTCACC ACCCTCTCCT GATGAGGTCC CCATCAATGT CAAGCAAGCG 
TACAAGACCT TCGCTGCAGT GCCCTTGTCA CACCCCCTGC TTGGGACACA GGAATTGCCT 
GGTCAGAGCG GCACAGAGAA TCCCAGAAGT GCCCCGGAGG TGGCTGCGCA CAGCCCTGGG 
GGACTGCACA GCCCTGAGCA GAAGTCCTTC CGTGAGAGGC AGAAGTATTT CGAGATTGAG 
GTGAAGCAGC AGCAGATGGA CAAGCCCCCC AAGAGAGTCT CCTTGGTGGG AGAGGACGAC 
CTGAAGAAGA TGAAAGAGGA GGAAGCTCGA AAACTGCAGC AGAAACGTAC CCTTCTGCTG 
GAGGAAGAGG CAGAAGAGGA GGAGATGGCG AAGCAGGCGC CTGAGGTGAG CGTGCAGTCC 
AGTGTCATCA TTGAAGGAGT CGAGTACAAG ATTGAGAGAC TCAACGGGAG GAGCAGCCAG 
TCTCCGGCCA CTCGGCCCTC CGAGGTCAAC GAGAACAGCA GCTCGCAGAG GAATTCACTG 
GAAGAGGGGA TCAAGGCTGA GCAGAGAACC AACTCCATGT CTGGGCTGAG TCCCTTGTAC 
CTTGGAGCAG GGGACGCAGT GGCACCCGTG CGGACGGCCA AAGCTGAACG GCGACACCAG 
GAGCGGTTGC GAATGCAGAG TCCTGAGCTG CTGAGTGGCC AAGAGAAGGA GCTTTCTCCT 
GCAGAACGTC GTGCTCTGGA GGCGGAGAAA CGAGCAATGT GGAGGGCAGC ACGATCCTCA 
GCTCTTGAAG ACAGTATTAA ACAGTACGAG CAGGACCTGG CCAGGAGGCT GTACCAGTCC 
CGACAGCGGG CACCTGCTCC CGGGGCCGGG CCAGCTCCGA TGCCTAGTCC CAAGCAGAGT 
CACGCCTCGG CACCCCCTGG GTCAGGAAGC CATTCCAGGA TGAAATCGCT TGAACAGGAT 
GCTCTGAAAG CCCAGATGGT CATTGCCAAG TCCAAGGAGG GGAAGAAGCG CAGCCCGCTG 
GAGCAGCTGG CAGAGTCCCC CTCGCCAGTT CCTACACCGT CACCGACACC ACTGGAAGAT 
TGCAGTCCCA GGAACGTCAC CTCCCCAGGA AGGCTGTCCA TGTCTGAAAA GAAGTTTGAC 
TACCGGGAAT TTGCTGCTAT TCCTTCCTCC AAACCCGTTT ACGAAATTCA GTCGCCTGAT 
GCTGCTGATG ACCTCAGATA CATCGAGGAC AGGAATAACT CAGTTCCCCA GGAGCAGGAT 
GGGCAGCCCG AGGAAGAGGA CACATTAGGA ACTCACGACT CATCCACACC CACTCCATCA 
GCACTTGAAG AAATGGCCCT GTACAGCAGC AAACGCAAGC TCCGACACAG CCGGCGCAGC 
CTGGAGGCTG CCGTCCCTAC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCRIB ( XM_014945734.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014801220.1 Calidris pugnax scribbled planar cell polarity protein (SCRIB), transcript variant X20, mRNA.

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CloneID	OCa67766
Clone ID Related Accession (Same CDS sequence)	XM_014945734.1
Accession Version	XM_014945734.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5664bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	protein scribble homolog isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090871.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCCGC TCTGGCGCTG CAACCGGCAC GTGGAGGCGC TGGACCGGCG GCACTGCTCG 
CTGCAGGCCG TGCCCGAGGA GATCTACCGC TACAGCCGCT CGCTGGAGGA GCTGCTGCTG 
GACGCCAACC AGCTGCGGGA GCTGCCCAAG CCTTTCTTCA GGCTTCTGAA CCTGCGCAAG 
CTGGGCCTGA GTGACAATGA GATCCAAAGA CTTCCCCCTG AGGTGGCCAA CTTCATGCAG 
CTGGTGGAAC TGGACATCTC CCGTAACGAC ATTCCAGAAA TTCCTGAAAG CATCAAATTC 
TGCAAATCCT TGGAAATCGC AGACTTCAGT GGGAATCCTC TCTCCAGGCT TCCCGAGGGC 
TTCACGCAGC TTCGGAGCCT GGGCCATTTG GCCCTGAATG ATGTGTCCCT GCAGAGCCTC 
CCCAGTGACA TTGGGAATTT GGCAAACCTG GTGACTCTGG AGCTACGCGA GAACCTGCTA 
AAGACTCTCC CCACCTCGCT GTCCTTCCTT GTCAAGTTAG AGCAGCTGGA TCTCGGTGGC 
AATGACCTGG AGGTTCTGCC AGACACTCTG GGAGCGCTCC CCAACCTGCG TGAGCTCTGG 
TTAGACCGGA ACCAGCTCTC AGCCCTGCCT CCCGAGCTGG GCAACCTCCG CCGCCTGGTC 
TGCCTGGATG TGTCGGAGAA CAAGCTGGAG CAGTTGCCCA ACGAGGTCAG CGGCCTGGTG 
GCACTGACCG ACTTGCTGCT GTCGCAGAAC CTGTTGGAGT GCATTCCTGA TGGGATTGGT 
CAGCTGAAGC AGCTCTCCAT CCTCAAGGTG GACCAGAATC GACTGACAGA GGTGACAGAG 
TCCATTGGGG ACTGTGAAAA TCTGTCCGAG CTCATCCTGA CAGAGAACAT GCTGACGGCC 
TTGCCGAAAT CCCTTGGGAA GCTGGCCAAG CTGACCAACC TGAACGTGGA TCGGAACCGG 
CTGACATCCC TCCCGGCCGA GATCGGTGGC TGTGCCAACC TCAACGTGCT GTCCTTGCGG 
GACAATCGCC TGACCCTCCT GCCATCGGAG CTGGCCAACA CCACCGAGCT TCATGTTCTG 
GATGTGGCTG GGAACAGGCT GCAGAACTTG CCGTTTGCTC TGACAAACCT CAACCTGAAA 
GCCCTGTGGC TGGCGGAGAA TCAGTCCCAG CCCATGCTGA AATTCCAAAC AGAGGATGAT 
GAGAAGACAG GAGAGAAAGT TCTCACTTGT TACCTGCTTC CTCAGCAGCC CTCTCCCAGC 
CTGGAGAACC TTCTACAGAA CAGCGTGGAC GAGAGCTGGA CGGACACCAA TTTAAACAGA 
GTCAGTGTGA TTCAGTTCCT GGATGAACCC AAAGCAGATG ATGAAGAAGA GTCTGGAGCT 
GAGAGAAGGG GCTTGCAGCG CAGAGCAACC CCCCATCCCA GCGAGCTGAA GGTGATGAAG 
AAGGTGATTG AAGTTCGGCG CAGTGAGGCC CACGCTGCAA AGCCCAATGC AGACCTGAAT 
TCTCCTCACT CAGAAGAGAA GAGGCTGAGC GCCACGTCCA ACCGCAGCGA GGACTCCCAC 
CTTTCCAACA GCACTGCCTC TGCTGACTGT AGGGCGGAGG AGCAGCAGGA GGAGGCAGAG 
AGGAGTCGGC CAAACGGGCA AGTGCCTGAG ATGCAGGAGC TGGAGAAGGA GGCAAAGTCC 
AGAGTGGACG AGGAGCACAA GGAAGCTGCT GGCCATGAAG AGGAAGATGT GGAAGTGGAA 
TACAATGAGC CCACTGTACA CTTTGCTGAA GACACGCTAA TACGGAGTGA GGATGAAGAT 
GAAGATGAAG AGCGACCGTT CCCAGTGGAG AAGCAGCGGC TCATCCGCAA GGACACCCCT 
CACTATAAGA AACACTTTAA GATAACTAAA TTGCCCAAGC CAGAGGCGGT GGTGGCCCTG 
CTGCAGGGGC TGAACAGCGA GGGGGTCCCC AAGGAGGAAG AGGAGTTGGG AGGCTGCAAC 
AACAACACAG AGCCTGCAGA CCAGGAGGAG GTGTGGGATG AGGAGGACCG GAAGAATGGA 
GCTTTGTCTG CCCAGCCATC GGTGAAGGGG GTGTCGTTTG ATCAAGCCAA TAATCTGCTG 
ATTGAACCTG CTCGCATTGA GGAGGAAGAG TTGACTCTGA CTATCGTACG GCAGACGGGG 
GGGCTGGGCA TCAGCATCGC AGGGGGCAAG GGCTCCACCC CCTATAAGGG CGATGATGAG 
GGCATCTTTA TTTCTCGGGT GTCAGAAGAA GGTCCTGCGG CTCGTGCAGG GGTTCGTGTT 
GGGGACAAGC TCCTGGAGGT GAACGGTGTG TCCCTGCACT CCGCTGAGCA CCACGTGGCA 
GTGGAGGCTC TGCGTGGATC GGGAAGTTCC GTCTCCATGA CTGTTCTGCG GGAGCGGATG 
GTGGAGCCGG AGAATGCCAT CACTGTCACC CCGCTGCGCC CCGAGGATGA CTACAGCCCC 
CGTGAGAGGC GAGGTGGCCT GCGCTTCCCG GAGCGACCCG AGGGGACACC TCCCACCGAG 
AGATACTCCA CGTGCCTCAT GCGCAACGAG AAGGGACTGG GCTTCAGTAT CGCTGGTGGC 
AAAGGCTCCA CTCCTTACCG GGCTGGTGAC ATGGGGATCT TTATCTCCCG GATCGCAGAG 
GGTGGTGCAG CCCATCGGGA CGGGATCCTG CACGTAGGAG ATCGGGTCAT CTCGATCAAT 
GGGGTAGACA TGACAGAAGC CAGGCATGAT CAGGCAGTAG CGCTGCTTAC CGCGTCCTCC 
CCCACCATCG TGCTGCTGGT AGAGAGAGAG GGTGCGGAGC AGCCCGGCGA GGGAGACTGG 
CCCGGGGGGC CACGGGCACG CATGCACTCC CCACCGCCAC CCCCCAGCCA TGGGGAGAGC 
CCGCCCGAGG AGACGCCCGC ACCACAGAGG AACCATCTCT CCAAAGGCCT GGAGGATCAG 
TACCCCATTG AGGAAATTCA CCTGGTGAAA GCAGGTGGTC CCCTGGGGCT CAGCATCGTA 
GGGGGCAGTG ACCACTCAAG CCATCCGTTT GGGATTCATG AACCAGGTGT CTTCATTTCG 
AAGGTCATTC CCCGTGGGCT GGCATCCCGC AGTGGGCTGC GAGTAGGGGA CAGGATCCTG 
GAAGTCAATA GCATTGACCT GCGCCACGCT ACCCACCAGG AAGCGGTGAA CGCCCTGCTC 
TCCAACACCC AGGAGCTGAC CATGTTAGTG AGGCGAGACC CACCACCACC TGGTATGCAG 
GAAATATGCA TTGAGAAGGC TCCAGGAGAA AAGCTGGGCA TCAGCATCCG GGGTGGAGCG 
AAAGGCCATG CTGGAAATCC GTTTGATCCC ACTGACGAGG GCATCTTCAT CTCTAAGGTG 
AGCTCCTCGG GGGCGGCAGC CCGGGACGGC CGCCTGAAGG TGGGGATGCG GATCCTAGAG 
GTGAACCACC AGAGCCTGCT GGGGATGACG CACACGGAAG CGGTGCAGAT GCTGCGCGGG 
GTGGGCGACG CGCTCCTGGT GCTGGTGTGT GACGGCTTCG ACCCCAAGGC TGCAGCTGCC 
ATCGAGATGT CCCCGGGAAT TATTGCAAAC CCTTTCGCTG CTGGTATCGG ACGCAAGAAT 
AGCCTGGAAA GTATCTCTTC CATCGACCGG GATTTAAGCC CTGAGGAACT GGAGATCCTG 
CAGAAGGAGA TGGAAATGGT GAGAGAAGCA ACTCAGTGGG AGAGAGAAGA AATGGAGAAA 
GTGAACGTCA CCAGCGAGCC CTTGAGACTG GACTATCGGA CCCTGGCTGC GCTGCCGAGC 
AGTGGTCTCC AGAGAGTCAA CCGCACCCTC GTTCAGTATA ATAAGCCCAG CTCCAGAATG 
GAGGGCGACT GTGCTCTGCT GTCTCTGCCG GTGGCACGGG ACAGCCGGGA TTCATGCCCT 
GACACGGCTG CTGGCCCCAC CATCGGAAGC GTGGCCAGAC CTCCCCGGGA CTCCGCAACA 
CCCGACCACT GCACCAGCGG GGCAGAAACG CCCCGGCCTG ACCAGCAGCC TCCCACAGGT 
GGCCCCTTGT CCCAGGACCA GCCGCCAAAG ACGCCAGAGC AAGAGTGCAT GGTGGACTCC 
CAGCCCATCC TCTTCAGTGA GAACCCCTTT GTGGTGGCCA ACCGCAGGGG GAAGGCGGCC 
GGCCGGGCCT GCCTGGGGGG GCCTCCCCTG GGCTATGGCA GGGGGGGAGT GCTGAAGACC 
AACCTTTACT CCAAGGCTCC TGCAGGCGAG TCTGGGATGG GCAATTCCAG GCGTGAAACC 
CCCTATTCTC CTGGTAACCA GCAGCGGCCT GCAACCCCTC GGCCCGCTCA CCTGCCGGGC 
AGGGAGACCC GCTTTGCTTC CATTAACTTC ATTACATCGC AGGATGACAG TAAATCGACC 
AAGCCAGGCG TCATTCAGCC TCTTTCCCGA GTGCGACAAA ATGCTGCCCC GCACAATTCG 
GAGGAGGGGG ACAGCCCCAA TCCCTTCCAG CAGCCCGAGC CGTGCTTCAG TGTCCAGAAC 
TCTCCAAAGC CACCCTCACC ACCCTCTCCT GATGAGGTCC CCATCAATGT CAAGCAAGCG 
TACAAGACCT TCGCTGCAGT GCCCTTGTCA CACCCCCTGC TTGGGACACA GGAATTGCCT 
GGTCAGAGCG GCACAGAGAA TCCCAGAAGT GCCCCGGAGG TGGCTGCGCA CAGCCCTGGG 
GGACTGCACA GCCCTGAGCA GAAGTCCTTC CGTGAGAGGC AGAAGTATTT CGAGATTGAG 
GTGAAGCAGC AGCAGATGGA CAAGCCCCCC AAGAGAGTCT CCTTGGTGGG AGAGGACGAC 
CTGAAGAAGA TGAAAGAGGA GGAAGCTCGA AAACTGCAGC AGAAACGTAC CCTTCTGCTG 
GAGGAAGAGG CAGAAGAGGA GGAGATGGCG AAGCAGGCGC CTGAGGTGAG CGTGCAGTCC 
AGTGTCATCA TTGAAGGAGT CGAGTACAAG ATTGAGAGAC TCAACGGGAG GAGCAGCCAG 
TCTCCGGCCA CTCGGCCCTC CGAGGTCAAC GAGAACAGCA GCTCGCAGAG GAATTCACTG 
GAAGAGGGGA TCAAGGCTGA GCAGAGAACC AACTCCATGT CTGGGCTGAG TCCCTTGTAC 
CTTGGAGCAG GGGACGCAGT GGCACCCGTG CGGACGGCCA AAGCTGAACG GCGACACCAG 
GAGCGGTTGC GAATGCAGAG TCCTGAGCTG CTGAGTGGCC AAGAGAAGGA GCTTTCTCCT 
GCAGAACGTC GTGCTCTGGA GGCGGAGAAA CGAGCAATGT GGAGGGCAGC ACGGATGAAA 
TCGCTTGAAC AGGATGCTCT GAAAGCCCAG ATGGTCATTG CCAAGTCCAA GGAGGGGAAG 
AAGCGCAGCC CGCTGGAGCA GCTGGCAGAG TCCCCCTCGC CAGTTCCTAC ACCGTCACCG 
ACACCACTGG AAGATTGCAG TCCCAGGAAC GTCACCTCCC CAGGAAGGCT GTCGCCTGAT 
GCTGCTGATG ACCTCAGATA CATCGAGGAC AGGAATAACT CAGTTCCCCA GGAGCAGGAT 
GGGCAGCCCG AGGAAGAGGA CACATTAGGA ACTCACGACT CATCCACACC CACTCCATCA 
GCACTTGAAG AAATGGCCCT GTACAGCAGC AAACGCAAGC TCCGACACAG CCGGCGCAGC 
CTGGAGGCTG CCGTCCCTAC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014801220.1
CDS	6..5669
Translation	MPPLWRCNRHVEALDRRHCSLQAVPEEIYRYSRSLEELLLDANQLRELPKPFFRLLNLRKLGLSDNEIQRLPPEVANFMQLVELDISRNDIPEIPESIKFCKSLEIADFSGNPLSRLPEGFTQLRSLGHLALNDVSLQSLPSDIGNLANLVTLELRENLLKTLPTSLSFLVKLEQLDLGGNDLEVLPDTLGALPNLRELWLDRNQLSALPPELGNLRRLVCLDVSENKLEQLPNEVSGLVALTDLLLSQNLLECIPDGIGQLKQLSILKVDQNRLTEVTESIGDCENLSELILTENMLTALPKSLGKLAKLTNLNVDRNRLTSLPAEIGGCANLNVLSLRDNRLTLLPSELANTTELHVLDVAGNRLQNLPFALTNLNLKALWLAENQSQPMLKFQTEDDEKTGEKVLTCYLLPQQPSPSLENLLQNSVDESWTDTNLNRVSVIQFLDEPKADDEEESGAERRGLQRRATPHPSELKVMKKVIEVRRSEAHAAKPNADLNSPHSEEKRLSATSNRSEDSHLSNSTASADCRAEEQQEEAERSRPNGQVPEMQELEKEAKSRVDEEHKEAAGHEEEDVEVEYNEPTVHFAEDTLIRSEDEDEDEERPFPVEKQRLIRKDTPHYKKHFKITKLPKPEAVVALLQGLNSEGVPKEEEELGGCNNNTEPADQEEVWDEEDRKNGALSAQPSVKGVSFDQANNLLIEPARIEEEELTLTIVRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVRVGDKLLEVNGVSLHSAEHHVAVEALRGSGSSVSMTVLRERMVEPENAITVTPLRPEDDYSPRERRGGLRFPERPEGTPPTERYSTCLMRNEKGLGFSIAGGKGSTPYRAGDMGIFISRIAEGGAAHRDGILHVGDRVISINGVDMTEARHDQAVALLTASSPTIVLLVEREGAEQPGEGDWPGGPRARMHSPPPPPSHGESPPEETPAPQRNHLSKGLEDQYPIEEIHLVKAGGPLGLSIVGGSDHSSHPFGIHEPGVFISKVIPRGLASRSGLRVGDRILEVNSIDLRHATHQEAVNALLSNTQELTMLVRRDPPPPGMQEICIEKAPGEKLGISIRGGAKGHAGNPFDPTDEGIFISKVSSSGAAARDGRLKVGMRILEVNHQSLLGMTHTEAVQMLRGVGDALLVLVCDGFDPKAAAAIEMSPGIIANPFAAGIGRKNSLESISSIDRDLSPEELEILQKEMEMVREATQWEREEMEKVNVTSEPLRLDYRTLAALPSSGLQRVNRTLVQYNKPSSRMEGDCALLSLPVARDSRDSCPDTAAGPTIGSVARPPRDSATPDHCTSGAETPRPDQQPPTGGPLSQDQPPKTPEQECMVDSQPILFSENPFVVANRRGKAAGRACLGGPPLGYGRGGVLKTNLYSKAPAGESGMGNSRRETPYSPGNQQRPATPRPAHLPGRETRFASINFITSQDDSKSTKPGVIQPLSRVRQNAAPHNSEEGDSPNPFQQPEPCFSVQNSPKPPSPPSPDEVPINVKQAYKTFAAVPLSHPLLGTQELPGQSGTENPRSAPEVAAHSPGGLHSPEQKSFRERQKYFEIEVKQQQMDKPPKRVSLVGEDDLKKMKEEEARKLQQKRTLLLEEEAEEEEMAKQAPEVSVQSSVIIEGVEYKIERLNGRSSQSPATRPSEVNENSSSQRNSLEEGIKAEQRTNSMSGLSPLYLGAGDAVAPVRTAKAERRHQERLRMQSPELLSGQEKELSPAERRALEAEKRAMWRAARMKSLEQDALKAQMVIAKSKEGKKRSPLEQLAESPSPVPTPSPTPLEDCSPRNVTSPGRLSPDAADDLRYIEDRNNSVPQEQDGQPEEEDTLGTHDSSTPTPSALEEMALYSSKRKLRHSRRSLEAAVPT

Target ORF information:

RefSeq Version	XM_014945734.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax scribbled planar cell polarity protein (SCRIB), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014945734.1

ORF Insert Sequence:

ATGCCGCCGC TCTGGCGCTG CAACCGGCAC GTGGAGGCGC TGGACCGGCG GCACTGCTCG 
CTGCAGGCCG TGCCCGAGGA GATCTACCGC TACAGCCGCT CGCTGGAGGA GCTGCTGCTG 
GACGCCAACC AGCTGCGGGA GCTGCCCAAG CCTTTCTTCA GGCTTCTGAA CCTGCGCAAG 
CTGGGCCTGA GTGACAATGA GATCCAAAGA CTTCCCCCTG AGGTGGCCAA CTTCATGCAG 
CTGGTGGAAC TGGACATCTC CCGTAACGAC ATTCCAGAAA TTCCTGAAAG CATCAAATTC 
TGCAAATCCT TGGAAATCGC AGACTTCAGT GGGAATCCTC TCTCCAGGCT TCCCGAGGGC 
TTCACGCAGC TTCGGAGCCT GGGCCATTTG GCCCTGAATG ATGTGTCCCT GCAGAGCCTC 
CCCAGTGACA TTGGGAATTT GGCAAACCTG GTGACTCTGG AGCTACGCGA GAACCTGCTA 
AAGACTCTCC CCACCTCGCT GTCCTTCCTT GTCAAGTTAG AGCAGCTGGA TCTCGGTGGC 
AATGACCTGG AGGTTCTGCC AGACACTCTG GGAGCGCTCC CCAACCTGCG TGAGCTCTGG 
TTAGACCGGA ACCAGCTCTC AGCCCTGCCT CCCGAGCTGG GCAACCTCCG CCGCCTGGTC 
TGCCTGGATG TGTCGGAGAA CAAGCTGGAG CAGTTGCCCA ACGAGGTCAG CGGCCTGGTG 
GCACTGACCG ACTTGCTGCT GTCGCAGAAC CTGTTGGAGT GCATTCCTGA TGGGATTGGT 
CAGCTGAAGC AGCTCTCCAT CCTCAAGGTG GACCAGAATC GACTGACAGA GGTGACAGAG 
TCCATTGGGG ACTGTGAAAA TCTGTCCGAG CTCATCCTGA CAGAGAACAT GCTGACGGCC 
TTGCCGAAAT CCCTTGGGAA GCTGGCCAAG CTGACCAACC TGAACGTGGA TCGGAACCGG 
CTGACATCCC TCCCGGCCGA GATCGGTGGC TGTGCCAACC TCAACGTGCT GTCCTTGCGG 
GACAATCGCC TGACCCTCCT GCCATCGGAG CTGGCCAACA CCACCGAGCT TCATGTTCTG 
GATGTGGCTG GGAACAGGCT GCAGAACTTG CCGTTTGCTC TGACAAACCT CAACCTGAAA 
GCCCTGTGGC TGGCGGAGAA TCAGTCCCAG CCCATGCTGA AATTCCAAAC AGAGGATGAT 
GAGAAGACAG GAGAGAAAGT TCTCACTTGT TACCTGCTTC CTCAGCAGCC CTCTCCCAGC 
CTGGAGAACC TTCTACAGAA CAGCGTGGAC GAGAGCTGGA CGGACACCAA TTTAAACAGA 
GTCAGTGTGA TTCAGTTCCT GGATGAACCC AAAGCAGATG ATGAAGAAGA GTCTGGAGCT 
GAGAGAAGGG GCTTGCAGCG CAGAGCAACC CCCCATCCCA GCGAGCTGAA GGTGATGAAG 
AAGGTGATTG AAGTTCGGCG CAGTGAGGCC CACGCTGCAA AGCCCAATGC AGACCTGAAT 
TCTCCTCACT CAGAAGAGAA GAGGCTGAGC GCCACGTCCA ACCGCAGCGA GGACTCCCAC 
CTTTCCAACA GCACTGCCTC TGCTGACTGT AGGGCGGAGG AGCAGCAGGA GGAGGCAGAG 
AGGAGTCGGC CAAACGGGCA AGTGCCTGAG ATGCAGGAGC TGGAGAAGGA GGCAAAGTCC 
AGAGTGGACG AGGAGCACAA GGAAGCTGCT GGCCATGAAG AGGAAGATGT GGAAGTGGAA 
TACAATGAGC CCACTGTACA CTTTGCTGAA GACACGCTAA TACGGAGTGA GGATGAAGAT 
GAAGATGAAG AGCGACCGTT CCCAGTGGAG AAGCAGCGGC TCATCCGCAA GGACACCCCT 
CACTATAAGA AACACTTTAA GATAACTAAA TTGCCCAAGC CAGAGGCGGT GGTGGCCCTG 
CTGCAGGGGC TGAACAGCGA GGGGGTCCCC AAGGAGGAAG AGGAGTTGGG AGGCTGCAAC 
AACAACACAG AGCCTGCAGA CCAGGAGGAG GTGTGGGATG AGGAGGACCG GAAGAATGGA 
GCTTTGTCTG CCCAGCCATC GGTGAAGGGG GTGTCGTTTG ATCAAGCCAA TAATCTGCTG 
ATTGAACCTG CTCGCATTGA GGAGGAAGAG TTGACTCTGA CTATCGTACG GCAGACGGGG 
GGGCTGGGCA TCAGCATCGC AGGGGGCAAG GGCTCCACCC CCTATAAGGG CGATGATGAG 
GGCATCTTTA TTTCTCGGGT GTCAGAAGAA GGTCCTGCGG CTCGTGCAGG GGTTCGTGTT 
GGGGACAAGC TCCTGGAGGT GAACGGTGTG TCCCTGCACT CCGCTGAGCA CCACGTGGCA 
GTGGAGGCTC TGCGTGGATC GGGAAGTTCC GTCTCCATGA CTGTTCTGCG GGAGCGGATG 
GTGGAGCCGG AGAATGCCAT CACTGTCACC CCGCTGCGCC CCGAGGATGA CTACAGCCCC 
CGTGAGAGGC GAGGTGGCCT GCGCTTCCCG GAGCGACCCG AGGGGACACC TCCCACCGAG 
AGATACTCCA CGTGCCTCAT GCGCAACGAG AAGGGACTGG GCTTCAGTAT CGCTGGTGGC 
AAAGGCTCCA CTCCTTACCG GGCTGGTGAC ATGGGGATCT TTATCTCCCG GATCGCAGAG 
GGTGGTGCAG CCCATCGGGA CGGGATCCTG CACGTAGGAG ATCGGGTCAT CTCGATCAAT 
GGGGTAGACA TGACAGAAGC CAGGCATGAT CAGGCAGTAG CGCTGCTTAC CGCGTCCTCC 
CCCACCATCG TGCTGCTGGT AGAGAGAGAG GGTGCGGAGC AGCCCGGCGA GGGAGACTGG 
CCCGGGGGGC CACGGGCACG CATGCACTCC CCACCGCCAC CCCCCAGCCA TGGGGAGAGC 
CCGCCCGAGG AGACGCCCGC ACCACAGAGG AACCATCTCT CCAAAGGCCT GGAGGATCAG 
TACCCCATTG AGGAAATTCA CCTGGTGAAA GCAGGTGGTC CCCTGGGGCT CAGCATCGTA 
GGGGGCAGTG ACCACTCAAG CCATCCGTTT GGGATTCATG AACCAGGTGT CTTCATTTCG 
AAGGTCATTC CCCGTGGGCT GGCATCCCGC AGTGGGCTGC GAGTAGGGGA CAGGATCCTG 
GAAGTCAATA GCATTGACCT GCGCCACGCT ACCCACCAGG AAGCGGTGAA CGCCCTGCTC 
TCCAACACCC AGGAGCTGAC CATGTTAGTG AGGCGAGACC CACCACCACC TGGTATGCAG 
GAAATATGCA TTGAGAAGGC TCCAGGAGAA AAGCTGGGCA TCAGCATCCG GGGTGGAGCG 
AAAGGCCATG CTGGAAATCC GTTTGATCCC ACTGACGAGG GCATCTTCAT CTCTAAGGTG 
AGCTCCTCGG GGGCGGCAGC CCGGGACGGC CGCCTGAAGG TGGGGATGCG GATCCTAGAG 
GTGAACCACC AGAGCCTGCT GGGGATGACG CACACGGAAG CGGTGCAGAT GCTGCGCGGG 
GTGGGCGACG CGCTCCTGGT GCTGGTGTGT GACGGCTTCG ACCCCAAGGC TGCAGCTGCC 
ATCGAGATGT CCCCGGGAAT TATTGCAAAC CCTTTCGCTG CTGGTATCGG ACGCAAGAAT 
AGCCTGGAAA GTATCTCTTC CATCGACCGG GATTTAAGCC CTGAGGAACT GGAGATCCTG 
CAGAAGGAGA TGGAAATGGT GAGAGAAGCA ACTCAGTGGG AGAGAGAAGA AATGGAGAAA 
GTGAACGTCA CCAGCGAGCC CTTGAGACTG GACTATCGGA CCCTGGCTGC GCTGCCGAGC 
AGTGGTCTCC AGAGAGTCAA CCGCACCCTC GTTCAGTATA ATAAGCCCAG CTCCAGAATG 
GAGGGCGACT GTGCTCTGCT GTCTCTGCCG GTGGCACGGG ACAGCCGGGA TTCATGCCCT 
GACACGGCTG CTGGCCCCAC CATCGGAAGC GTGGCCAGAC CTCCCCGGGA CTCCGCAACA 
CCCGACCACT GCACCAGCGG GGCAGAAACG CCCCGGCCTG ACCAGCAGCC TCCCACAGGT 
GGCCCCTTGT CCCAGGACCA GCCGCCAAAG ACGCCAGAGC AAGAGTGCAT GGTGGACTCC 
CAGCCCATCC TCTTCAGTGA GAACCCCTTT GTGGTGGCCA ACCGCAGGGG GAAGGCGGCC 
GGCCGGGCCT GCCTGGGGGG GCCTCCCCTG GGCTATGGCA GGGGGGGAGT GCTGAAGACC 
AACCTTTACT CCAAGGCTCC TGCAGGCGAG TCTGGGATGG GCAATTCCAG GCGTGAAACC 
CCCTATTCTC CTGGTAACCA GCAGCGGCCT GCAACCCCTC GGCCCGCTCA CCTGCCGGGC 
AGGGAGACCC GCTTTGCTTC CATTAACTTC ATTACATCGC AGGATGACAG TAAATCGACC 
AAGCCAGGCG TCATTCAGCC TCTTTCCCGA GTGCGACAAA ATGCTGCCCC GCACAATTCG 
GAGGAGGGGG ACAGCCCCAA TCCCTTCCAG CAGCCCGAGC CGTGCTTCAG TGTCCAGAAC 
TCTCCAAAGC CACCCTCACC ACCCTCTCCT GATGAGGTCC CCATCAATGT CAAGCAAGCG 
TACAAGACCT TCGCTGCAGT GCCCTTGTCA CACCCCCTGC TTGGGACACA GGAATTGCCT 
GGTCAGAGCG GCACAGAGAA TCCCAGAAGT GCCCCGGAGG TGGCTGCGCA CAGCCCTGGG 
GGACTGCACA GCCCTGAGCA GAAGTCCTTC CGTGAGAGGC AGAAGTATTT CGAGATTGAG 
GTGAAGCAGC AGCAGATGGA CAAGCCCCCC AAGAGAGTCT CCTTGGTGGG AGAGGACGAC 
CTGAAGAAGA TGAAAGAGGA GGAAGCTCGA AAACTGCAGC AGAAACGTAC CCTTCTGCTG 
GAGGAAGAGG CAGAAGAGGA GGAGATGGCG AAGCAGGCGC CTGAGGTGAG CGTGCAGTCC 
AGTGTCATCA TTGAAGGAGT CGAGTACAAG ATTGAGAGAC TCAACGGGAG GAGCAGCCAG 
TCTCCGGCCA CTCGGCCCTC CGAGGTCAAC GAGAACAGCA GCTCGCAGAG GAATTCACTG 
GAAGAGGGGA TCAAGGCTGA GCAGAGAACC AACTCCATGT CTGGGCTGAG TCCCTTGTAC 
CTTGGAGCAG GGGACGCAGT GGCACCCGTG CGGACGGCCA AAGCTGAACG GCGACACCAG 
GAGCGGTTGC GAATGCAGAG TCCTGAGCTG CTGAGTGGCC AAGAGAAGGA GCTTTCTCCT 
GCAGAACGTC GTGCTCTGGA GGCGGAGAAA CGAGCAATGT GGAGGGCAGC ACGGATGAAA 
TCGCTTGAAC AGGATGCTCT GAAAGCCCAG ATGGTCATTG CCAAGTCCAA GGAGGGGAAG 
AAGCGCAGCC CGCTGGAGCA GCTGGCAGAG TCCCCCTCGC CAGTTCCTAC ACCGTCACCG 
ACACCACTGG AAGATTGCAG TCCCAGGAAC GTCACCTCCC CAGGAAGGCT GTCGCCTGAT 
GCTGCTGATG ACCTCAGATA CATCGAGGAC AGGAATAACT CAGTTCCCCA GGAGCAGGAT 
GGGCAGCCCG AGGAAGAGGA CACATTAGGA ACTCACGACT CATCCACACC CACTCCATCA 
GCACTTGAAG AAATGGCCCT GTACAGCAGC AAACGCAAGC TCCGACACAG CCGGCGCAGC 
CTGGAGGCTG CCGTCCCTAC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	SCRIB
Entrez Gene ID	106890125
Full Name	scribbled planar cell polarity protein
Gene Type	protein-coding
Organism	Calidris pugnax(ruff)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

SCRIB cDNA ORF clone, Calidris pugnax(ruff)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

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Target ORF information:

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