TMCC1 cDNA ORF clone, Calidris pugnax(ruff)

The following TMCC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TMCC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCa68529 XM_014946757.1
Latest version!
Calidris pugnax transmembrane and coiled-coil domain family 1 (TMCC1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $258.30
$369.00
OCa68530 XM_014946758.1
Latest version!
Calidris pugnax transmembrane and coiled-coil domain family 1 (TMCC1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $258.30
$369.00
OCa68527 XM_014946754.1
Latest version!
Calidris pugnax transmembrane and coiled-coil domain family 1 (TMCC1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00
OCa68527 XM_014946755.1
Latest version!
Calidris pugnax transmembrane and coiled-coil domain family 1 (TMCC1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00
OCa68527 XM_014946753.1
Latest version!
Calidris pugnax transmembrane and coiled-coil domain family 1 (TMCC1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00
OCa68528 XM_014946756.1
Latest version!
Calidris pugnax transmembrane and coiled-coil domain family 1 (TMCC1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCa68529
    Clone ID Related Accession (Same CDS sequence) XM_014946757.1
    Accession Version XM_014946757.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1425bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product transmembrane and coiled-coil domains protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090880.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGTGCAGC GCTTCAGCCT CCGCCGGCAG CTCTCCAAGA TCGAGCGGTT GGAAGTCAGC 
    AGCCTCGCAC AGACCTCCAG TGCAGTGGCC TCCAGCACCG ATGGCAGCAT CAACGCCGAT
    TCTGTTGATG GGACCCCAGA CCCTCAGCGG ACAAAAGTGG CTATCACACA CCTACAGCAG
    AAGATACTGA AGTTGACCGA GCAGATCAAA ATTGAACAAA CAGCCCGTGA TGACAACGTG
    GCAGAGTACC TGAAACTGGC CAACAACGCA GACAAGCAGC AGAGTGCCCG CATTAAGCAA
    GTGTTTGAGA AGAAAAACCA AAAGTCAGCA CAGACCATCT TGCAGCTGCA GAAGAAACTA
    GAACATTACC ATCGAAAGCT GCGAGAGATT GAGCAGAATG GGATCCCTCG GCAGCCGAAG
    GATGTCTTCA GGGATATGCA CCAGGGTTTG AAAGATGTTG GAGCAAAAGT CACTGGCTTC
    AGTGAGGGAG TAGTCGACAG CGTCAAAGGT GGGCTTTCCA GTTTCTCCCA AGCCACACAC
    TCGGCAGCAG GAGCTGTGGT TTCCAAGCCC CGGGAGATTG CCTCCCTCAT AAGGAACAAG
    TTTGGGAGTG CAGACAACAT TGCTAATCTG AAAGACTCCT TAGAAGAAGG CCAGGAAGAT
    GGGACGGGAG GCAAGGCTCT AGGTGTTATT CAGAACTTTC AGTCGAGTCC AAAATATGGC
    AGTGAAGAGG ATTGCTCCAG TGCCACGTCG GGCTCAGTGG GAGCCAACAG CACAACAGGG
    GGCCCTGTGG GAGCTTCCAG CTCCAAAACA AACACTCTGG ATATGCAGAG CTCAGGGTTT
    GATGCAATAC TGCATGAGAT TCAAGAAATT CGAGAGACAC AGTCAAGACT GGAAGAATCA
    TTTGAGGACC TTAAGGTTCG CTATCAGAAG GATTACTCAT TAATAATGCA GACGCTGCAG
    GAGGAGCGGT ACAGATGCGA GAGACTGGAA GAGCAGCTGA ATGACCTGAC TGAGCTCCAC
    CAGAACGAGA TCCTTAATTT AAAGCAGGAG TTGGCCAGCA TGGAGGAGAA AATCGCCTAC
    CAGTCTTACG AGCGAGCACG GGACATCCAG GAGGCTCTGG AGGCCTGCCA GACCCGCATC
    TCCAAGATGG AGCTGCAGCA GCAGCAGCAG CAGGTGGTGC AGCTGGAGGG GCTGGAGAAT
    GCCACGGCCA GGAACCTGCT GGGGAAGTTC ATCAACATCC TCCTGGCCGT CATGGCTGTC
    CTCCTCGTCT TTGTCTCCAC CGTGGCCAAC TGCGTTGTGC CCCTCATGAA GACTCGCAAT
    AGGACGTTCA GCACTTTATT TATAGTGGTT TTCATTGCCT TTTTGTGGAA GCACTGGGAC
    GCCATCACCG GCTACTTGGA ACGGTTCTTG TCCCCCCCCA GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014802243.1
    CDS90..1514
    Translation

    Target ORF information:

    RefSeq Version XM_014946757.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax transmembrane and coiled-coil domain family 1 (TMCC1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014946757.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGTGCAGC GCTTCAGCCT CCGCCGGCAG CTCTCCAAGA TCGAGCGGTT GGAAGTCAGC 
    AGCCTCGCAC AGACCTCCAG TGCAGTGGCC TCCAGCACCG ATGGCAGCAT CAACGCCGAT
    TCTGTTGATG GGACCCCAGA CCCTCAGCGG ACAAAAGTGG CTATCACACA CCTACAGCAG
    AAGATACTGA AGTTGACCGA GCAGATCAAA ATTGAACAAA CAGCCCGTGA TGACAACGTG
    GCAGAGTACC TGAAACTGGC CAACAACGCA GACAAGCAGC AGAGTGCCCG CATTAAGCAA
    GTGTTTGAGA AGAAAAACCA AAAGTCAGCA CAGACCATCT TGCAGCTGCA GAAGAAACTA
    GAACATTACC ATCGAAAGCT GCGAGAGATT GAGCAGAATG GGATCCCTCG GCAGCCGAAG
    GATGTCTTCA GGGATATGCA CCAGGGTTTG AAAGATGTTG GAGCAAAAGT CACTGGCTTC
    AGTGAGGGAG TAGTCGACAG CGTCAAAGGT GGGCTTTCCA GTTTCTCCCA AGCCACACAC
    TCGGCAGCAG GAGCTGTGGT TTCCAAGCCC CGGGAGATTG CCTCCCTCAT AAGGAACAAG
    TTTGGGAGTG CAGACAACAT TGCTAATCTG AAAGACTCCT TAGAAGAAGG CCAGGAAGAT
    GGGACGGGAG GCAAGGCTCT AGGTGTTATT CAGAACTTTC AGTCGAGTCC AAAATATGGC
    AGTGAAGAGG ATTGCTCCAG TGCCACGTCG GGCTCAGTGG GAGCCAACAG CACAACAGGG
    GGCCCTGTGG GAGCTTCCAG CTCCAAAACA AACACTCTGG ATATGCAGAG CTCAGGGTTT
    GATGCAATAC TGCATGAGAT TCAAGAAATT CGAGAGACAC AGTCAAGACT GGAAGAATCA
    TTTGAGGACC TTAAGGTTCG CTATCAGAAG GATTACTCAT TAATAATGCA GACGCTGCAG
    GAGGAGCGGT ACAGATGCGA GAGACTGGAA GAGCAGCTGA ATGACCTGAC TGAGCTCCAC
    CAGAACGAGA TCCTTAATTT AAAGCAGGAG TTGGCCAGCA TGGAGGAGAA AATCGCCTAC
    CAGTCTTACG AGCGAGCACG GGACATCCAG GAGGCTCTGG AGGCCTGCCA GACCCGCATC
    TCCAAGATGG AGCTGCAGCA GCAGCAGCAG CAGGTGGTGC AGCTGGAGGG GCTGGAGAAT
    GCCACGGCCA GGAACCTGCT GGGGAAGTTC ATCAACATCC TCCTGGCCGT CATGGCTGTC
    CTCCTCGTCT TTGTCTCCAC CGTGGCCAAC TGCGTTGTGC CCCTCATGAA GACTCGCAAT
    AGGACGTTCA GCACTTTATT TATAGTGGTT TTCATTGCCT TTTTGTGGAA GCACTGGGAC
    GCCATCACCG GCTACTTGGA ACGGTTCTTG TCCCCCCCCA GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa68530
    Clone ID Related Accession (Same CDS sequence) XM_014946758.1
    Accession Version XM_014946758.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1416bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product transmembrane and coiled-coil domains protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090880.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGAGGGCT CCCCGCCTGC CGCCCAAACC ATCGAGCGGT TGGAAGTCAG CAGCCTCGCA 
    CAGACCTCCA GTGCAGTGGC CTCCAGCACC GATGGCAGCA TCAACGCCGA TTCTGTTGAT
    GGGACCCCAG ACCCTCAGCG GACAAAAGTG GCTATCACAC ACCTACAGCA GAAGATACTG
    AAGTTGACCG AGCAGATCAA AATTGAACAA ACAGCCCGTG ATGACAACGT GGCAGAGTAC
    CTGAAACTGG CCAACAACGC AGACAAGCAG CAGAGTGCCC GCATTAAGCA AGTGTTTGAG
    AAGAAAAACC AAAAGTCAGC ACAGACCATC TTGCAGCTGC AGAAGAAACT AGAACATTAC
    CATCGAAAGC TGCGAGAGAT TGAGCAGAAT GGGATCCCTC GGCAGCCGAA GGATGTCTTC
    AGGGATATGC ACCAGGGTTT GAAAGATGTT GGAGCAAAAG TCACTGGCTT CAGTGAGGGA
    GTAGTCGACA GCGTCAAAGG TGGGCTTTCC AGTTTCTCCC AAGCCACACA CTCGGCAGCA
    GGAGCTGTGG TTTCCAAGCC CCGGGAGATT GCCTCCCTCA TAAGGAACAA GTTTGGGAGT
    GCAGACAACA TTGCTAATCT GAAAGACTCC TTAGAAGAAG GCCAGGAAGA TGGGACGGGA
    GGCAAGGCTC TAGGTGTTAT TCAGAACTTT CAGTCGAGTC CAAAATATGG CAGTGAAGAG
    GATTGCTCCA GTGCCACGTC GGGCTCAGTG GGAGCCAACA GCACAACAGG GGGCCCTGTG
    GGAGCTTCCA GCTCCAAAAC AAACACTCTG GATATGCAGA GCTCAGGGTT TGATGCAATA
    CTGCATGAGA TTCAAGAAAT TCGAGAGACA CAGTCAAGAC TGGAAGAATC ATTTGAGGAC
    CTTAAGGTTC GCTATCAGAA GGATTACTCA TTAATAATGC AGACGCTGCA GGAGGAGCGG
    TACAGATGCG AGAGACTGGA AGAGCAGCTG AATGACCTGA CTGAGCTCCA CCAGAACGAG
    ATCCTTAATT TAAAGCAGGA GTTGGCCAGC ATGGAGGAGA AAATCGCCTA CCAGTCTTAC
    GAGCGAGCAC GGGACATCCA GGAGGCTCTG GAGGCCTGCC AGACCCGCAT CTCCAAGATG
    GAGCTGCAGC AGCAGCAGCA GCAGGTGGTG CAGCTGGAGG GGCTGGAGAA TGCCACGGCC
    AGGAACCTGC TGGGGAAGTT CATCAACATC CTCCTGGCCG TCATGGCTGT CCTCCTCGTC
    TTTGTCTCCA CCGTGGCCAA CTGCGTTGTG CCCCTCATGA AGACTCGCAA TAGGACGTTC
    AGCACTTTAT TTATAGTGGT TTTCATTGCC TTTTTGTGGA AGCACTGGGA CGCCATCACC
    GGCTACTTGG AACGGTTCTT GTCCCCCCCC AGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014802244.1
    CDS35..1450
    Translation

    Target ORF information:

    RefSeq Version XM_014946758.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax transmembrane and coiled-coil domain family 1 (TMCC1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014946758.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGAGGGCT CCCCGCCTGC CGCCCAAACC ATCGAGCGGT TGGAAGTCAG CAGCCTCGCA 
    CAGACCTCCA GTGCAGTGGC CTCCAGCACC GATGGCAGCA TCAACGCCGA TTCTGTTGAT
    GGGACCCCAG ACCCTCAGCG GACAAAAGTG GCTATCACAC ACCTACAGCA GAAGATACTG
    AAGTTGACCG AGCAGATCAA AATTGAACAA ACAGCCCGTG ATGACAACGT GGCAGAGTAC
    CTGAAACTGG CCAACAACGC AGACAAGCAG CAGAGTGCCC GCATTAAGCA AGTGTTTGAG
    AAGAAAAACC AAAAGTCAGC ACAGACCATC TTGCAGCTGC AGAAGAAACT AGAACATTAC
    CATCGAAAGC TGCGAGAGAT TGAGCAGAAT GGGATCCCTC GGCAGCCGAA GGATGTCTTC
    AGGGATATGC ACCAGGGTTT GAAAGATGTT GGAGCAAAAG TCACTGGCTT CAGTGAGGGA
    GTAGTCGACA GCGTCAAAGG TGGGCTTTCC AGTTTCTCCC AAGCCACACA CTCGGCAGCA
    GGAGCTGTGG TTTCCAAGCC CCGGGAGATT GCCTCCCTCA TAAGGAACAA GTTTGGGAGT
    GCAGACAACA TTGCTAATCT GAAAGACTCC TTAGAAGAAG GCCAGGAAGA TGGGACGGGA
    GGCAAGGCTC TAGGTGTTAT TCAGAACTTT CAGTCGAGTC CAAAATATGG CAGTGAAGAG
    GATTGCTCCA GTGCCACGTC GGGCTCAGTG GGAGCCAACA GCACAACAGG GGGCCCTGTG
    GGAGCTTCCA GCTCCAAAAC AAACACTCTG GATATGCAGA GCTCAGGGTT TGATGCAATA
    CTGCATGAGA TTCAAGAAAT TCGAGAGACA CAGTCAAGAC TGGAAGAATC ATTTGAGGAC
    CTTAAGGTTC GCTATCAGAA GGATTACTCA TTAATAATGC AGACGCTGCA GGAGGAGCGG
    TACAGATGCG AGAGACTGGA AGAGCAGCTG AATGACCTGA CTGAGCTCCA CCAGAACGAG
    ATCCTTAATT TAAAGCAGGA GTTGGCCAGC ATGGAGGAGA AAATCGCCTA CCAGTCTTAC
    GAGCGAGCAC GGGACATCCA GGAGGCTCTG GAGGCCTGCC AGACCCGCAT CTCCAAGATG
    GAGCTGCAGC AGCAGCAGCA GCAGGTGGTG CAGCTGGAGG GGCTGGAGAA TGCCACGGCC
    AGGAACCTGC TGGGGAAGTT CATCAACATC CTCCTGGCCG TCATGGCTGT CCTCCTCGTC
    TTTGTCTCCA CCGTGGCCAA CTGCGTTGTG CCCCTCATGA AGACTCGCAA TAGGACGTTC
    AGCACTTTAT TTATAGTGGT TTTCATTGCC TTTTTGTGGA AGCACTGGGA CGCCATCACC
    GGCTACTTGG AACGGTTCTT GTCCCCCCCC AGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa68527
    Clone ID Related Accession (Same CDS sequence) XM_014946754.1 , XM_014946755.1 , XM_014946753.1
    Accession Version XM_014946754.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1932bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product transmembrane and coiled-coil domains protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090880.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGTCCT CTGGCACTGA ACAGTTGTGT GATGACCCTG ATCCTGGAGG CAAATCCCAA 
    GATCAAGAGA CCAAAAAACA ACATGAATCA GAGCAGAAAT TGTCCAAAAT AACCCATAAT
    GCTTTGGAGA ACATTAATGT GATTGGTCAA GGCTTGAAGC ACCTTTTCCA GCATCAGCGC
    AGGAGGTCAT CAGTCTCTCC GCACGATGTG CAGCAAGCTC AGGCTGATCT GGAGCCTGAC
    GTGGATCTGG AAAGCCAAAG CGTCTGTGCT GAAATTGATG GTGTCTCCAC CCACCCCACA
    GCTCTGAACC GGGTGCTCCA GCAGATAAGA GTGCCACCTA AGATGAAGAG AGGGACCAGC
    CTGCACAGCA GGCGGGGCAA GGCAGACGCC CCGAAGGGGA GCCCGCAGAT CAGCAGGAGG
    TCTGCCCAGG ACATCCAGTC GGGCCGGCCT AGATCCTCAT CGACTACAGA TGCGCCCACA
    AACTTGTCCG TGATGGAGAT TGCTTCTTCT ATCTACGTAG GTGGAGAGGA GGCCACGGCA
    GCAGCAATCG AGCGGTTGGA AGTCAGCAGC CTCGCACAGA CCTCCAGTGC AGTGGCCTCC
    AGCACCGATG GCAGCATCAA CGCCGATTCT GTTGATGGGA CCCCAGACCC TCAGCGGACA
    AAAGTGGCTA TCACACACCT ACAGCAGAAG ATACTGAAGT TGACCGAGCA GATCAAAATT
    GAACAAACAG CCCGTGATGA CAACGTGGCA GAGTACCTGA AACTGGCCAA CAACGCAGAC
    AAGCAGCAGA GTGCCCGCAT TAAGCAAGTG TTTGAGAAGA AAAACCAAAA GTCAGCACAG
    ACCATCTTGC AGCTGCAGAA GAAACTAGAA CATTACCATC GAAAGCTGCG AGAGATTGAG
    CAGAATGGGA TCCCTCGGCA GCCGAAGGAT GTCTTCAGGG ATATGCACCA GGGTTTGAAA
    GATGTTGGAG CAAAAGTCAC TGGCTTCAGT GAGGGAGTAG TCGACAGCGT CAAAGGTGGG
    CTTTCCAGTT TCTCCCAAGC CACACACTCG GCAGCAGGAG CTGTGGTTTC CAAGCCCCGG
    GAGATTGCCT CCCTCATAAG GAACAAGTTT GGGAGTGCAG ACAACATTGC TAATCTGAAA
    GACTCCTTAG AAGAAGGCCA GGAAGATGGG ACGGGAGGCA AGGCTCTAGG TGTTATTCAG
    AACTTTCAGT CGAGTCCAAA ATATGGCAGT GAAGAGGATT GCTCCAGTGC CACGTCGGGC
    TCAGTGGGAG CCAACAGCAC AACAGGGGGC CCTGTGGGAG CTTCCAGCTC CAAAACAAAC
    ACTCTGGATA TGCAGAGCTC AGGGTTTGAT GCAATACTGC ATGAGATTCA AGAAATTCGA
    GAGACACAGT CAAGACTGGA AGAATCATTT GAGGACCTTA AGGTTCGCTA TCAGAAGGAT
    TACTCATTAA TAATGCAGAC GCTGCAGGAG GAGCGGTACA GATGCGAGAG ACTGGAAGAG
    CAGCTGAATG ACCTGACTGA GCTCCACCAG AACGAGATCC TTAATTTAAA GCAGGAGTTG
    GCCAGCATGG AGGAGAAAAT CGCCTACCAG TCTTACGAGC GAGCACGGGA CATCCAGGAG
    GCTCTGGAGG CCTGCCAGAC CCGCATCTCC AAGATGGAGC TGCAGCAGCA GCAGCAGCAG
    GTGGTGCAGC TGGAGGGGCT GGAGAATGCC ACGGCCAGGA ACCTGCTGGG GAAGTTCATC
    AACATCCTCC TGGCCGTCAT GGCTGTCCTC CTCGTCTTTG TCTCCACCGT GGCCAACTGC
    GTTGTGCCCC TCATGAAGAC TCGCAATAGG ACGTTCAGCA CTTTATTTAT AGTGGTTTTC
    ATTGCCTTTT TGTGGAAGCA CTGGGACGCC ATCACCGGCT ACTTGGAACG GTTCTTGTCC
    CCCCCCAGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014802240.1
    CDS1501..3432
    Translation

    Target ORF information:

    RefSeq Version XM_014946754.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax transmembrane and coiled-coil domain family 1 (TMCC1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014946754.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGTCCT CTGGCACTGA ACAGTTGTGT GATGACCCTG ATCCTGGAGG CAAATCCCAA 
    GATCAAGAGA CCAAAAAACA ACATGAATCA GAGCAGAAAT TGTCCAAAAT AACCCATAAT
    GCTTTGGAGA ACATTAATGT GATTGGTCAA GGCTTGAAGC ACCTTTTCCA GCATCAGCGC
    AGGAGGTCAT CAGTCTCTCC GCACGATGTG CAGCAAGCTC AGGCTGATCT GGAGCCTGAC
    GTGGATCTGG AAAGCCAAAG CGTCTGTGCT GAAATTGATG GTGTCTCCAC CCACCCCACA
    GCTCTGAACC GGGTGCTCCA GCAGATAAGA GTGCCACCTA AGATGAAGAG AGGGACCAGC
    CTGCACAGCA GGCGGGGCAA GGCAGACGCC CCGAAGGGGA GCCCGCAGAT CAGCAGGAGG
    TCTGCCCAGG ACATCCAGTC GGGCCGGCCT AGATCCTCAT CGACTACAGA TGCGCCCACA
    AACTTGTCCG TGATGGAGAT TGCTTCTTCT ATCTACGTAG GTGGAGAGGA GGCCACGGCA
    GCAGCAATCG AGCGGTTGGA AGTCAGCAGC CTCGCACAGA CCTCCAGTGC AGTGGCCTCC
    AGCACCGATG GCAGCATCAA CGCCGATTCT GTTGATGGGA CCCCAGACCC TCAGCGGACA
    AAAGTGGCTA TCACACACCT ACAGCAGAAG ATACTGAAGT TGACCGAGCA GATCAAAATT
    GAACAAACAG CCCGTGATGA CAACGTGGCA GAGTACCTGA AACTGGCCAA CAACGCAGAC
    AAGCAGCAGA GTGCCCGCAT TAAGCAAGTG TTTGAGAAGA AAAACCAAAA GTCAGCACAG
    ACCATCTTGC AGCTGCAGAA GAAACTAGAA CATTACCATC GAAAGCTGCG AGAGATTGAG
    CAGAATGGGA TCCCTCGGCA GCCGAAGGAT GTCTTCAGGG ATATGCACCA GGGTTTGAAA
    GATGTTGGAG CAAAAGTCAC TGGCTTCAGT GAGGGAGTAG TCGACAGCGT CAAAGGTGGG
    CTTTCCAGTT TCTCCCAAGC CACACACTCG GCAGCAGGAG CTGTGGTTTC CAAGCCCCGG
    GAGATTGCCT CCCTCATAAG GAACAAGTTT GGGAGTGCAG ACAACATTGC TAATCTGAAA
    GACTCCTTAG AAGAAGGCCA GGAAGATGGG ACGGGAGGCA AGGCTCTAGG TGTTATTCAG
    AACTTTCAGT CGAGTCCAAA ATATGGCAGT GAAGAGGATT GCTCCAGTGC CACGTCGGGC
    TCAGTGGGAG CCAACAGCAC AACAGGGGGC CCTGTGGGAG CTTCCAGCTC CAAAACAAAC
    ACTCTGGATA TGCAGAGCTC AGGGTTTGAT GCAATACTGC ATGAGATTCA AGAAATTCGA
    GAGACACAGT CAAGACTGGA AGAATCATTT GAGGACCTTA AGGTTCGCTA TCAGAAGGAT
    TACTCATTAA TAATGCAGAC GCTGCAGGAG GAGCGGTACA GATGCGAGAG ACTGGAAGAG
    CAGCTGAATG ACCTGACTGA GCTCCACCAG AACGAGATCC TTAATTTAAA GCAGGAGTTG
    GCCAGCATGG AGGAGAAAAT CGCCTACCAG TCTTACGAGC GAGCACGGGA CATCCAGGAG
    GCTCTGGAGG CCTGCCAGAC CCGCATCTCC AAGATGGAGC TGCAGCAGCA GCAGCAGCAG
    GTGGTGCAGC TGGAGGGGCT GGAGAATGCC ACGGCCAGGA ACCTGCTGGG GAAGTTCATC
    AACATCCTCC TGGCCGTCAT GGCTGTCCTC CTCGTCTTTG TCTCCACCGT GGCCAACTGC
    GTTGTGCCCC TCATGAAGAC TCGCAATAGG ACGTTCAGCA CTTTATTTAT AGTGGTTTTC
    ATTGCCTTTT TGTGGAAGCA CTGGGACGCC ATCACCGGCT ACTTGGAACG GTTCTTGTCC
    CCCCCCAGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa68527
    Clone ID Related Accession (Same CDS sequence) XM_014946754.1 , XM_014946755.1 , XM_014946753.1
    Accession Version XM_014946755.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1932bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product transmembrane and coiled-coil domains protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090880.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGTCCT CTGGCACTGA ACAGTTGTGT GATGACCCTG ATCCTGGAGG CAAATCCCAA 
    GATCAAGAGA CCAAAAAACA ACATGAATCA GAGCAGAAAT TGTCCAAAAT AACCCATAAT
    GCTTTGGAGA ACATTAATGT GATTGGTCAA GGCTTGAAGC ACCTTTTCCA GCATCAGCGC
    AGGAGGTCAT CAGTCTCTCC GCACGATGTG CAGCAAGCTC AGGCTGATCT GGAGCCTGAC
    GTGGATCTGG AAAGCCAAAG CGTCTGTGCT GAAATTGATG GTGTCTCCAC CCACCCCACA
    GCTCTGAACC GGGTGCTCCA GCAGATAAGA GTGCCACCTA AGATGAAGAG AGGGACCAGC
    CTGCACAGCA GGCGGGGCAA GGCAGACGCC CCGAAGGGGA GCCCGCAGAT CAGCAGGAGG
    TCTGCCCAGG ACATCCAGTC GGGCCGGCCT AGATCCTCAT CGACTACAGA TGCGCCCACA
    AACTTGTCCG TGATGGAGAT TGCTTCTTCT ATCTACGTAG GTGGAGAGGA GGCCACGGCA
    GCAGCAATCG AGCGGTTGGA AGTCAGCAGC CTCGCACAGA CCTCCAGTGC AGTGGCCTCC
    AGCACCGATG GCAGCATCAA CGCCGATTCT GTTGATGGGA CCCCAGACCC TCAGCGGACA
    AAAGTGGCTA TCACACACCT ACAGCAGAAG ATACTGAAGT TGACCGAGCA GATCAAAATT
    GAACAAACAG CCCGTGATGA CAACGTGGCA GAGTACCTGA AACTGGCCAA CAACGCAGAC
    AAGCAGCAGA GTGCCCGCAT TAAGCAAGTG TTTGAGAAGA AAAACCAAAA GTCAGCACAG
    ACCATCTTGC AGCTGCAGAA GAAACTAGAA CATTACCATC GAAAGCTGCG AGAGATTGAG
    CAGAATGGGA TCCCTCGGCA GCCGAAGGAT GTCTTCAGGG ATATGCACCA GGGTTTGAAA
    GATGTTGGAG CAAAAGTCAC TGGCTTCAGT GAGGGAGTAG TCGACAGCGT CAAAGGTGGG
    CTTTCCAGTT TCTCCCAAGC CACACACTCG GCAGCAGGAG CTGTGGTTTC CAAGCCCCGG
    GAGATTGCCT CCCTCATAAG GAACAAGTTT GGGAGTGCAG ACAACATTGC TAATCTGAAA
    GACTCCTTAG AAGAAGGCCA GGAAGATGGG ACGGGAGGCA AGGCTCTAGG TGTTATTCAG
    AACTTTCAGT CGAGTCCAAA ATATGGCAGT GAAGAGGATT GCTCCAGTGC CACGTCGGGC
    TCAGTGGGAG CCAACAGCAC AACAGGGGGC CCTGTGGGAG CTTCCAGCTC CAAAACAAAC
    ACTCTGGATA TGCAGAGCTC AGGGTTTGAT GCAATACTGC ATGAGATTCA AGAAATTCGA
    GAGACACAGT CAAGACTGGA AGAATCATTT GAGGACCTTA AGGTTCGCTA TCAGAAGGAT
    TACTCATTAA TAATGCAGAC GCTGCAGGAG GAGCGGTACA GATGCGAGAG ACTGGAAGAG
    CAGCTGAATG ACCTGACTGA GCTCCACCAG AACGAGATCC TTAATTTAAA GCAGGAGTTG
    GCCAGCATGG AGGAGAAAAT CGCCTACCAG TCTTACGAGC GAGCACGGGA CATCCAGGAG
    GCTCTGGAGG CCTGCCAGAC CCGCATCTCC AAGATGGAGC TGCAGCAGCA GCAGCAGCAG
    GTGGTGCAGC TGGAGGGGCT GGAGAATGCC ACGGCCAGGA ACCTGCTGGG GAAGTTCATC
    AACATCCTCC TGGCCGTCAT GGCTGTCCTC CTCGTCTTTG TCTCCACCGT GGCCAACTGC
    GTTGTGCCCC TCATGAAGAC TCGCAATAGG ACGTTCAGCA CTTTATTTAT AGTGGTTTTC
    ATTGCCTTTT TGTGGAAGCA CTGGGACGCC ATCACCGGCT ACTTGGAACG GTTCTTGTCC
    CCCCCCAGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014802241.1
    CDS2760..4691
    Translation

    Target ORF information:

    RefSeq Version XM_014946755.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax transmembrane and coiled-coil domain family 1 (TMCC1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014946755.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGTCCT CTGGCACTGA ACAGTTGTGT GATGACCCTG ATCCTGGAGG CAAATCCCAA 
    GATCAAGAGA CCAAAAAACA ACATGAATCA GAGCAGAAAT TGTCCAAAAT AACCCATAAT
    GCTTTGGAGA ACATTAATGT GATTGGTCAA GGCTTGAAGC ACCTTTTCCA GCATCAGCGC
    AGGAGGTCAT CAGTCTCTCC GCACGATGTG CAGCAAGCTC AGGCTGATCT GGAGCCTGAC
    GTGGATCTGG AAAGCCAAAG CGTCTGTGCT GAAATTGATG GTGTCTCCAC CCACCCCACA
    GCTCTGAACC GGGTGCTCCA GCAGATAAGA GTGCCACCTA AGATGAAGAG AGGGACCAGC
    CTGCACAGCA GGCGGGGCAA GGCAGACGCC CCGAAGGGGA GCCCGCAGAT CAGCAGGAGG
    TCTGCCCAGG ACATCCAGTC GGGCCGGCCT AGATCCTCAT CGACTACAGA TGCGCCCACA
    AACTTGTCCG TGATGGAGAT TGCTTCTTCT ATCTACGTAG GTGGAGAGGA GGCCACGGCA
    GCAGCAATCG AGCGGTTGGA AGTCAGCAGC CTCGCACAGA CCTCCAGTGC AGTGGCCTCC
    AGCACCGATG GCAGCATCAA CGCCGATTCT GTTGATGGGA CCCCAGACCC TCAGCGGACA
    AAAGTGGCTA TCACACACCT ACAGCAGAAG ATACTGAAGT TGACCGAGCA GATCAAAATT
    GAACAAACAG CCCGTGATGA CAACGTGGCA GAGTACCTGA AACTGGCCAA CAACGCAGAC
    AAGCAGCAGA GTGCCCGCAT TAAGCAAGTG TTTGAGAAGA AAAACCAAAA GTCAGCACAG
    ACCATCTTGC AGCTGCAGAA GAAACTAGAA CATTACCATC GAAAGCTGCG AGAGATTGAG
    CAGAATGGGA TCCCTCGGCA GCCGAAGGAT GTCTTCAGGG ATATGCACCA GGGTTTGAAA
    GATGTTGGAG CAAAAGTCAC TGGCTTCAGT GAGGGAGTAG TCGACAGCGT CAAAGGTGGG
    CTTTCCAGTT TCTCCCAAGC CACACACTCG GCAGCAGGAG CTGTGGTTTC CAAGCCCCGG
    GAGATTGCCT CCCTCATAAG GAACAAGTTT GGGAGTGCAG ACAACATTGC TAATCTGAAA
    GACTCCTTAG AAGAAGGCCA GGAAGATGGG ACGGGAGGCA AGGCTCTAGG TGTTATTCAG
    AACTTTCAGT CGAGTCCAAA ATATGGCAGT GAAGAGGATT GCTCCAGTGC CACGTCGGGC
    TCAGTGGGAG CCAACAGCAC AACAGGGGGC CCTGTGGGAG CTTCCAGCTC CAAAACAAAC
    ACTCTGGATA TGCAGAGCTC AGGGTTTGAT GCAATACTGC ATGAGATTCA AGAAATTCGA
    GAGACACAGT CAAGACTGGA AGAATCATTT GAGGACCTTA AGGTTCGCTA TCAGAAGGAT
    TACTCATTAA TAATGCAGAC GCTGCAGGAG GAGCGGTACA GATGCGAGAG ACTGGAAGAG
    CAGCTGAATG ACCTGACTGA GCTCCACCAG AACGAGATCC TTAATTTAAA GCAGGAGTTG
    GCCAGCATGG AGGAGAAAAT CGCCTACCAG TCTTACGAGC GAGCACGGGA CATCCAGGAG
    GCTCTGGAGG CCTGCCAGAC CCGCATCTCC AAGATGGAGC TGCAGCAGCA GCAGCAGCAG
    GTGGTGCAGC TGGAGGGGCT GGAGAATGCC ACGGCCAGGA ACCTGCTGGG GAAGTTCATC
    AACATCCTCC TGGCCGTCAT GGCTGTCCTC CTCGTCTTTG TCTCCACCGT GGCCAACTGC
    GTTGTGCCCC TCATGAAGAC TCGCAATAGG ACGTTCAGCA CTTTATTTAT AGTGGTTTTC
    ATTGCCTTTT TGTGGAAGCA CTGGGACGCC ATCACCGGCT ACTTGGAACG GTTCTTGTCC
    CCCCCCAGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa68527
    Clone ID Related Accession (Same CDS sequence) XM_014946754.1 , XM_014946755.1 , XM_014946753.1
    Accession Version XM_014946753.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1932bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product transmembrane and coiled-coil domains protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090880.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGTCCT CTGGCACTGA ACAGTTGTGT GATGACCCTG ATCCTGGAGG CAAATCCCAA 
    GATCAAGAGA CCAAAAAACA ACATGAATCA GAGCAGAAAT TGTCCAAAAT AACCCATAAT
    GCTTTGGAGA ACATTAATGT GATTGGTCAA GGCTTGAAGC ACCTTTTCCA GCATCAGCGC
    AGGAGGTCAT CAGTCTCTCC GCACGATGTG CAGCAAGCTC AGGCTGATCT GGAGCCTGAC
    GTGGATCTGG AAAGCCAAAG CGTCTGTGCT GAAATTGATG GTGTCTCCAC CCACCCCACA
    GCTCTGAACC GGGTGCTCCA GCAGATAAGA GTGCCACCTA AGATGAAGAG AGGGACCAGC
    CTGCACAGCA GGCGGGGCAA GGCAGACGCC CCGAAGGGGA GCCCGCAGAT CAGCAGGAGG
    TCTGCCCAGG ACATCCAGTC GGGCCGGCCT AGATCCTCAT CGACTACAGA TGCGCCCACA
    AACTTGTCCG TGATGGAGAT TGCTTCTTCT ATCTACGTAG GTGGAGAGGA GGCCACGGCA
    GCAGCAATCG AGCGGTTGGA AGTCAGCAGC CTCGCACAGA CCTCCAGTGC AGTGGCCTCC
    AGCACCGATG GCAGCATCAA CGCCGATTCT GTTGATGGGA CCCCAGACCC TCAGCGGACA
    AAAGTGGCTA TCACACACCT ACAGCAGAAG ATACTGAAGT TGACCGAGCA GATCAAAATT
    GAACAAACAG CCCGTGATGA CAACGTGGCA GAGTACCTGA AACTGGCCAA CAACGCAGAC
    AAGCAGCAGA GTGCCCGCAT TAAGCAAGTG TTTGAGAAGA AAAACCAAAA GTCAGCACAG
    ACCATCTTGC AGCTGCAGAA GAAACTAGAA CATTACCATC GAAAGCTGCG AGAGATTGAG
    CAGAATGGGA TCCCTCGGCA GCCGAAGGAT GTCTTCAGGG ATATGCACCA GGGTTTGAAA
    GATGTTGGAG CAAAAGTCAC TGGCTTCAGT GAGGGAGTAG TCGACAGCGT CAAAGGTGGG
    CTTTCCAGTT TCTCCCAAGC CACACACTCG GCAGCAGGAG CTGTGGTTTC CAAGCCCCGG
    GAGATTGCCT CCCTCATAAG GAACAAGTTT GGGAGTGCAG ACAACATTGC TAATCTGAAA
    GACTCCTTAG AAGAAGGCCA GGAAGATGGG ACGGGAGGCA AGGCTCTAGG TGTTATTCAG
    AACTTTCAGT CGAGTCCAAA ATATGGCAGT GAAGAGGATT GCTCCAGTGC CACGTCGGGC
    TCAGTGGGAG CCAACAGCAC AACAGGGGGC CCTGTGGGAG CTTCCAGCTC CAAAACAAAC
    ACTCTGGATA TGCAGAGCTC AGGGTTTGAT GCAATACTGC ATGAGATTCA AGAAATTCGA
    GAGACACAGT CAAGACTGGA AGAATCATTT GAGGACCTTA AGGTTCGCTA TCAGAAGGAT
    TACTCATTAA TAATGCAGAC GCTGCAGGAG GAGCGGTACA GATGCGAGAG ACTGGAAGAG
    CAGCTGAATG ACCTGACTGA GCTCCACCAG AACGAGATCC TTAATTTAAA GCAGGAGTTG
    GCCAGCATGG AGGAGAAAAT CGCCTACCAG TCTTACGAGC GAGCACGGGA CATCCAGGAG
    GCTCTGGAGG CCTGCCAGAC CCGCATCTCC AAGATGGAGC TGCAGCAGCA GCAGCAGCAG
    GTGGTGCAGC TGGAGGGGCT GGAGAATGCC ACGGCCAGGA ACCTGCTGGG GAAGTTCATC
    AACATCCTCC TGGCCGTCAT GGCTGTCCTC CTCGTCTTTG TCTCCACCGT GGCCAACTGC
    GTTGTGCCCC TCATGAAGAC TCGCAATAGG ACGTTCAGCA CTTTATTTAT AGTGGTTTTC
    ATTGCCTTTT TGTGGAAGCA CTGGGACGCC ATCACCGGCT ACTTGGAACG GTTCTTGTCC
    CCCCCCAGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014802239.1
    CDS1738..3669
    Translation

    Target ORF information:

    RefSeq Version XM_014946753.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax transmembrane and coiled-coil domain family 1 (TMCC1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014946753.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGTCCT CTGGCACTGA ACAGTTGTGT GATGACCCTG ATCCTGGAGG CAAATCCCAA 
    GATCAAGAGA CCAAAAAACA ACATGAATCA GAGCAGAAAT TGTCCAAAAT AACCCATAAT
    GCTTTGGAGA ACATTAATGT GATTGGTCAA GGCTTGAAGC ACCTTTTCCA GCATCAGCGC
    AGGAGGTCAT CAGTCTCTCC GCACGATGTG CAGCAAGCTC AGGCTGATCT GGAGCCTGAC
    GTGGATCTGG AAAGCCAAAG CGTCTGTGCT GAAATTGATG GTGTCTCCAC CCACCCCACA
    GCTCTGAACC GGGTGCTCCA GCAGATAAGA GTGCCACCTA AGATGAAGAG AGGGACCAGC
    CTGCACAGCA GGCGGGGCAA GGCAGACGCC CCGAAGGGGA GCCCGCAGAT CAGCAGGAGG
    TCTGCCCAGG ACATCCAGTC GGGCCGGCCT AGATCCTCAT CGACTACAGA TGCGCCCACA
    AACTTGTCCG TGATGGAGAT TGCTTCTTCT ATCTACGTAG GTGGAGAGGA GGCCACGGCA
    GCAGCAATCG AGCGGTTGGA AGTCAGCAGC CTCGCACAGA CCTCCAGTGC AGTGGCCTCC
    AGCACCGATG GCAGCATCAA CGCCGATTCT GTTGATGGGA CCCCAGACCC TCAGCGGACA
    AAAGTGGCTA TCACACACCT ACAGCAGAAG ATACTGAAGT TGACCGAGCA GATCAAAATT
    GAACAAACAG CCCGTGATGA CAACGTGGCA GAGTACCTGA AACTGGCCAA CAACGCAGAC
    AAGCAGCAGA GTGCCCGCAT TAAGCAAGTG TTTGAGAAGA AAAACCAAAA GTCAGCACAG
    ACCATCTTGC AGCTGCAGAA GAAACTAGAA CATTACCATC GAAAGCTGCG AGAGATTGAG
    CAGAATGGGA TCCCTCGGCA GCCGAAGGAT GTCTTCAGGG ATATGCACCA GGGTTTGAAA
    GATGTTGGAG CAAAAGTCAC TGGCTTCAGT GAGGGAGTAG TCGACAGCGT CAAAGGTGGG
    CTTTCCAGTT TCTCCCAAGC CACACACTCG GCAGCAGGAG CTGTGGTTTC CAAGCCCCGG
    GAGATTGCCT CCCTCATAAG GAACAAGTTT GGGAGTGCAG ACAACATTGC TAATCTGAAA
    GACTCCTTAG AAGAAGGCCA GGAAGATGGG ACGGGAGGCA AGGCTCTAGG TGTTATTCAG
    AACTTTCAGT CGAGTCCAAA ATATGGCAGT GAAGAGGATT GCTCCAGTGC CACGTCGGGC
    TCAGTGGGAG CCAACAGCAC AACAGGGGGC CCTGTGGGAG CTTCCAGCTC CAAAACAAAC
    ACTCTGGATA TGCAGAGCTC AGGGTTTGAT GCAATACTGC ATGAGATTCA AGAAATTCGA
    GAGACACAGT CAAGACTGGA AGAATCATTT GAGGACCTTA AGGTTCGCTA TCAGAAGGAT
    TACTCATTAA TAATGCAGAC GCTGCAGGAG GAGCGGTACA GATGCGAGAG ACTGGAAGAG
    CAGCTGAATG ACCTGACTGA GCTCCACCAG AACGAGATCC TTAATTTAAA GCAGGAGTTG
    GCCAGCATGG AGGAGAAAAT CGCCTACCAG TCTTACGAGC GAGCACGGGA CATCCAGGAG
    GCTCTGGAGG CCTGCCAGAC CCGCATCTCC AAGATGGAGC TGCAGCAGCA GCAGCAGCAG
    GTGGTGCAGC TGGAGGGGCT GGAGAATGCC ACGGCCAGGA ACCTGCTGGG GAAGTTCATC
    AACATCCTCC TGGCCGTCAT GGCTGTCCTC CTCGTCTTTG TCTCCACCGT GGCCAACTGC
    GTTGTGCCCC TCATGAAGAC TCGCAATAGG ACGTTCAGCA CTTTATTTAT AGTGGTTTTC
    ATTGCCTTTT TGTGGAAGCA CTGGGACGCC ATCACCGGCT ACTTGGAACG GTTCTTGTCC
    CCCCCCAGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa68528
    Clone ID Related Accession (Same CDS sequence) XM_014946756.1
    Accession Version XM_014946756.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1590bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product transmembrane and coiled-coil domains protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090880.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGAAGAGAG GGACCAGCCT GCACAGCAGG CGGGGCAAGG CAGACGCCCC GAAGGGGAGC 
    CCGCAGATCA GCAGGAGGTC TGCCCAGGAC ATCCAGTCGG GCCGGCCTAG ATCCTCATCG
    ACTACAGATG CGCCCACAAA CTTGTCCGTG ATGGAGATTG CTTCTTCTAT CTACGTAGGT
    GGAGAGGAGG CCACGGCAGC AGCAATCGAG CGGTTGGAAG TCAGCAGCCT CGCACAGACC
    TCCAGTGCAG TGGCCTCCAG CACCGATGGC AGCATCAACG CCGATTCTGT TGATGGGACC
    CCAGACCCTC AGCGGACAAA AGTGGCTATC ACACACCTAC AGCAGAAGAT ACTGAAGTTG
    ACCGAGCAGA TCAAAATTGA ACAAACAGCC CGTGATGACA ACGTGGCAGA GTACCTGAAA
    CTGGCCAACA ACGCAGACAA GCAGCAGAGT GCCCGCATTA AGCAAGTGTT TGAGAAGAAA
    AACCAAAAGT CAGCACAGAC CATCTTGCAG CTGCAGAAGA AACTAGAACA TTACCATCGA
    AAGCTGCGAG AGATTGAGCA GAATGGGATC CCTCGGCAGC CGAAGGATGT CTTCAGGGAT
    ATGCACCAGG GTTTGAAAGA TGTTGGAGCA AAAGTCACTG GCTTCAGTGA GGGAGTAGTC
    GACAGCGTCA AAGGTGGGCT TTCCAGTTTC TCCCAAGCCA CACACTCGGC AGCAGGAGCT
    GTGGTTTCCA AGCCCCGGGA GATTGCCTCC CTCATAAGGA ACAAGTTTGG GAGTGCAGAC
    AACATTGCTA ATCTGAAAGA CTCCTTAGAA GAAGGCCAGG AAGATGGGAC GGGAGGCAAG
    GCTCTAGGTG TTATTCAGAA CTTTCAGTCG AGTCCAAAAT ATGGCAGTGA AGAGGATTGC
    TCCAGTGCCA CGTCGGGCTC AGTGGGAGCC AACAGCACAA CAGGGGGCCC TGTGGGAGCT
    TCCAGCTCCA AAACAAACAC TCTGGATATG CAGAGCTCAG GGTTTGATGC AATACTGCAT
    GAGATTCAAG AAATTCGAGA GACACAGTCA AGACTGGAAG AATCATTTGA GGACCTTAAG
    GTTCGCTATC AGAAGGATTA CTCATTAATA ATGCAGACGC TGCAGGAGGA GCGGTACAGA
    TGCGAGAGAC TGGAAGAGCA GCTGAATGAC CTGACTGAGC TCCACCAGAA CGAGATCCTT
    AATTTAAAGC AGGAGTTGGC CAGCATGGAG GAGAAAATCG CCTACCAGTC TTACGAGCGA
    GCACGGGACA TCCAGGAGGC TCTGGAGGCC TGCCAGACCC GCATCTCCAA GATGGAGCTG
    CAGCAGCAGC AGCAGCAGGT GGTGCAGCTG GAGGGGCTGG AGAATGCCAC GGCCAGGAAC
    CTGCTGGGGA AGTTCATCAA CATCCTCCTG GCCGTCATGG CTGTCCTCCT CGTCTTTGTC
    TCCACCGTGG CCAACTGCGT TGTGCCCCTC ATGAAGACTC GCAATAGGAC GTTCAGCACT
    TTATTTATAG TGGTTTTCAT TGCCTTTTTG TGGAAGCACT GGGACGCCAT CACCGGCTAC
    TTGGAACGGT TCTTGTCCCC CCCCAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014802242.1
    CDS748..2337
    Translation

    Target ORF information:

    RefSeq Version XM_014946756.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax transmembrane and coiled-coil domain family 1 (TMCC1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014946756.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGAAGAGAG GGACCAGCCT GCACAGCAGG CGGGGCAAGG CAGACGCCCC GAAGGGGAGC 
    CCGCAGATCA GCAGGAGGTC TGCCCAGGAC ATCCAGTCGG GCCGGCCTAG ATCCTCATCG
    ACTACAGATG CGCCCACAAA CTTGTCCGTG ATGGAGATTG CTTCTTCTAT CTACGTAGGT
    GGAGAGGAGG CCACGGCAGC AGCAATCGAG CGGTTGGAAG TCAGCAGCCT CGCACAGACC
    TCCAGTGCAG TGGCCTCCAG CACCGATGGC AGCATCAACG CCGATTCTGT TGATGGGACC
    CCAGACCCTC AGCGGACAAA AGTGGCTATC ACACACCTAC AGCAGAAGAT ACTGAAGTTG
    ACCGAGCAGA TCAAAATTGA ACAAACAGCC CGTGATGACA ACGTGGCAGA GTACCTGAAA
    CTGGCCAACA ACGCAGACAA GCAGCAGAGT GCCCGCATTA AGCAAGTGTT TGAGAAGAAA
    AACCAAAAGT CAGCACAGAC CATCTTGCAG CTGCAGAAGA AACTAGAACA TTACCATCGA
    AAGCTGCGAG AGATTGAGCA GAATGGGATC CCTCGGCAGC CGAAGGATGT CTTCAGGGAT
    ATGCACCAGG GTTTGAAAGA TGTTGGAGCA AAAGTCACTG GCTTCAGTGA GGGAGTAGTC
    GACAGCGTCA AAGGTGGGCT TTCCAGTTTC TCCCAAGCCA CACACTCGGC AGCAGGAGCT
    GTGGTTTCCA AGCCCCGGGA GATTGCCTCC CTCATAAGGA ACAAGTTTGG GAGTGCAGAC
    AACATTGCTA ATCTGAAAGA CTCCTTAGAA GAAGGCCAGG AAGATGGGAC GGGAGGCAAG
    GCTCTAGGTG TTATTCAGAA CTTTCAGTCG AGTCCAAAAT ATGGCAGTGA AGAGGATTGC
    TCCAGTGCCA CGTCGGGCTC AGTGGGAGCC AACAGCACAA CAGGGGGCCC TGTGGGAGCT
    TCCAGCTCCA AAACAAACAC TCTGGATATG CAGAGCTCAG GGTTTGATGC AATACTGCAT
    GAGATTCAAG AAATTCGAGA GACACAGTCA AGACTGGAAG AATCATTTGA GGACCTTAAG
    GTTCGCTATC AGAAGGATTA CTCATTAATA ATGCAGACGC TGCAGGAGGA GCGGTACAGA
    TGCGAGAGAC TGGAAGAGCA GCTGAATGAC CTGACTGAGC TCCACCAGAA CGAGATCCTT
    AATTTAAAGC AGGAGTTGGC CAGCATGGAG GAGAAAATCG CCTACCAGTC TTACGAGCGA
    GCACGGGACA TCCAGGAGGC TCTGGAGGCC TGCCAGACCC GCATCTCCAA GATGGAGCTG
    CAGCAGCAGC AGCAGCAGGT GGTGCAGCTG GAGGGGCTGG AGAATGCCAC GGCCAGGAAC
    CTGCTGGGGA AGTTCATCAA CATCCTCCTG GCCGTCATGG CTGTCCTCCT CGTCTTTGTC
    TCCACCGTGG CCAACTGCGT TGTGCCCCTC ATGAAGACTC GCAATAGGAC GTTCAGCACT
    TTATTTATAG TGGTTTTCAT TGCCTTTTTG TGGAAGCACT GGGACGCCAT CACCGGCTAC
    TTGGAACGGT TCTTGTCCCC CCCCAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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