DHX8 cDNA ORF clone, Calidris pugnax(ruff)

Gene
Clones
gRNAs

Summary

Gene Symbol	DHX8
Entrez Gene ID	106898099
Full Name	DEAH-box helicase 8
Gene Type	protein-coding
Organism	Calidris pugnax(ruff)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014959184.1	XP_014814670.1	ATP-dependent RNA helicase DHX8

Pathways from BioSystems
Gene Ontology

The following DHX8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DHX8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCa77640	XM_014959184.1 Latest version!	Calidris pugnax DEAH (Asp-Glu-Ala-His) box polypeptide 8 (DHX8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DHX8 ( XM_014959184.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014814670.1 Calidris pugnax DEAH (Asp-Glu-Ala-His) box polypeptide 8 (DHX8), mRNA.

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CloneID	OCa77640
Clone ID Related Accession (Same CDS sequence)	XM_014959184.1
Accession Version	XM_014959184.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	ATP-dependent RNA helicase DHX8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091149.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGACC TGGGGACGCT GGAGGAGCTC GCGAAGCTCG AGTACCTGTC GCTGGTCTCC 
AAGGTGTGCA CCGAGCTCGA TAATCACCTG GGAATCAACG ACAAGGACCT GGCCGAGTTC 
GTGATAAGTC TTGCTGAGAA AAATACTACA TTTGACACAT TTAAAGCAGT TCTTCTAAAG 
AATGGGGCCG AGTTCACTGA TTCCCTTATT AGTAATTTGT TGCGTCTCAT ACAGACGATG 
CGGCCTCCAC CGAAAGCATC TACGAGCAAA GAGACAGTTG CCAAACCCAA ATCGGAGAAG 
GAAAAGTTGA GGGAATTGTT TCCAGCCCTT TGCAGGCCAG ACAATCCCAA CATCAGGAAT 
ATGTTGGATG AAGATGATGT CAAAGTGGCA GCTGATGCAC TTAAAGAACT CGAAGCTCTG 
ATGCCAAGTG CGGACAGGCA AGGCAAGCAG CGGAGCAGCG ATCACCGGGC GAAGAAAAAG 
AGGAGGAGTC GAAGTCGTAG CAGGGACGGG AGACGCCGGC ACAGATCTCG CTCGAGGTCT 
CGTTCTAGGA CTCGGGATAA GAACAGGGGA AAATCCAGAT ACAGGTCAAG GAGCAGAAGT 
TGGAGTCCCA GTAGGGACCG TAGGGATCGA GAGAAGTATG TTGAGAAGAG CAACGAGAGA 
TGGAGAGACA AACACATTGA CCGTCCACCA CCTGAGGAGC CTTCCATTGG AGACATTTAC 
AACGGCAAAG TCACCAGTAT AATGCAGTTT GGATGCTTTG TGCAGCTGGA AGGACTAAGG 
AAGCGTTGGG AGGGCTTGGT CCACATCTCA GAGCTTCGAA GAGAAGGACG GGTTGCCAAT 
GTTGCTGATG TTGTGAGCAA AGGACAGAGA GTAAAAGTCA AAGTGTTATC CTTTACTGGA 
TCCAAAACCA GCCTGAGCAT GAAGGATGTT GATCAAAGCA CTGGGGAAGA CTTGAACCCA 
AACCGGAGGA GAAACCTGGT TGGAGAAACT AATGAAGAAA TCTCGATGAG GAACCCAGAC 
AGACCCAGTC ACCTGTCCCT GGTGAACGCT CCGGAGGTGG AGGATGACAG CCTGGAACGG 
AAACGCCTTA CCCGAATTTC AGACCCAGAG AAGTGGGAGA TTAAACAGAT GATTGCAGCT 
AATGTGCTTT CCAAAGAAGA GTTTCCTGAC TTTGATGAGG AGACTGGGAT TCTTCCTAAA 
GTTGATGATG AAGAAGATGA GGACCTTGAA ATTGAGTTAG TTGAAGAAGA GCCACCCTTC 
CTTCGAGGTC ATACTAAACA GAGCATGGAT ATGAGTCCCA TCAAAATAGT CAAGAATCCA 
GATGGTTCCT TGTCCCAGGC TGCAATGATG CAGAGTGCTT TAGCTAAAGA AAGACGAGAA 
CTTAAACAAG CCCAGAGAGA GGCAGAGATG GATTCTATCC CCATGGGACT GAACACACAC 
TGGGTGGATC CTCTCCCTGA CGTGGATGGA AGACAAATTG CCGCAAACAT GCGGGGTATT 
GGGATGATGC CCAATGATAT CCCAGAGTGG AAGAAACATG CATTTGGTGG CAACAAAGCT 
TCTTATGGTA AGAAGACCCA GCTTTCCATC ATTGAGCAGA GAGAGAGTCT CCCAATCTTC 
AGACTGAAGG AGCAGCTGAT ACAAGCTGTG CATGACAACC AGATTCTGAT TGTTATTGGA 
GAGACAGGAT CTGGGAAAAC AACACAGATT ACCCAGTACC TGGCTGAGGC AGGTTACACA 
TCGAGAGGAA AGATTGGATG TACTCAGCCT CGCAGAGTGG CTGCAATGTC TGTTGCAAAG 
AGGGTGTCCG AGGAGTTTGG TTGCTGCTTG GGACAGGAGG TTGGCTACAC CATTCGATTT 
GAAGACTGCA CCAGCCCTGA AACTGTTATC AAATACATGA CAGATGGTAT GTTACTGAGG 
GAGTGCTTGA TAGATCCTGA CCTGACTCAA TATGCCATCA TCATGCTGGA TGAAGCCCAT 
GAGAGGACCA TACATACAGA TGTGCTCTTT GGGCTACTGA AAAAGACAGT GCAGAAACGG 
CAGGACATGA AGCTGATAGT GACCTCAGCC ACACTGGATG CTGTGAAATT TTCTCAGTAT 
TTCTACGAAG CACCAATCTT CACAATTCCT GGCAGAACAT ACCCAGTAGA AATTCTGTAC 
ACAAAAGAGC CAGAAACAGA TTATTTGGAT GCCAGTCTCA TTACAGTAAT GCAAATCCAC 
TTAACAGAGC CACCAGGTGA CATTCTGGTG TTTCTGACTG GTCAGGAAGA GATTGACACA 
GCCTGTGAAA TCCTCTATGA GAGGATGAAA TCTCTAGGAC CTGATGTTCC AGAGTTAATT 
ATCCTACCTG TATATTCAGC TTTACCCAGT GAAATGCAAA CCAGGATTTT TGACCCAGCC 
CCACCAGGAA GCAGAAAGGT TGTCATCGCT ACTAACATTG CTGAGACATC TTTGACTATT 
GATGGGATAT ATTACGTAGT GGATCCTGGC TTTGTGAAGC AGAAAGTTTA TAACTCGAAG 
ACTGGAATTG ACCAGCTGGT TGTTACACCA ATTTCACAGG CTCAAGCGAA GCAACGAGCA 
GGAAGGGCTG GGAGAACAGG ACCAGGAAAA TGCTATAGGT TATATACAGA ACGTGCTTAT 
CGAGATGAAA TGCTAACCAC CAATGTGCCT GAAATCCAGA GGACAAATCT GGCCAGTACA 
GTACTTTCCC TGAAGGCCAT GGGCATCAAT GATTTGCTCT CCTTTGACTT TATGGATGCT 
CCCCCGATGG AAACTCTTAT AACAGCCATG GAGCAGCTCT ACACCCTGGG AGCTCTGGAC 
GACGAGGGAC TGCTCACGCG ACTGGGGCGC AGGATGGCGG AATTCCCCTT GGAGCCTATG 
TTGTGTAAGA TGTTGATCAT GTCTGTCCAT CTGGGATGCA GTGAGGAAAT GCTGACAATA 
GTATCCATGC TGTCTGTACA GAATGTGTTC TACAGGCCAA AGGATAAACA AGCACTTGCT 
GATCAAAAGA AAGCCAAGTT CCATCAGACA GAAGGGGACC ACCTCACTCT GCTGGCTGTG 
TACAATTCCT GGAAGAATAA TAAGTTTTCA AATCCCTGGT GCTATGAAAA TTTTATCCAG 
GCCCGATCCT TACGCAGGGC ACAGGACATC CGCAAGCAGA TGCTGGGCAT TATGGACAGG 
CACAAGCTGG ATGTGGTATC CTGTGGGAAG GCAACAGTTC GAGTCCAGAA GGCCATCTGC 
AGTGGTTTCT TCAGAAATGC AGCAAAGAAG GATCCGCAGG AAGGTTATCG GACGCTCATT 
GACCAACAAG TAGTTTATAT CCACCCGTCC AGCGCGCTCT TCAACAGGCA GCCAGAATGG 
GTGGTGTATC ATGAACTGGT GCTGACCACC AAGGAATATA TGCGGGAAGT GACAACTATT 
GATCCTCGCT GGCTGGTTGA ATTTGCACCA GCTTTCTTCA AGGTTTCTGA TCCAACCAAG 
CTGAGCAAAC AGAAGAAGCA GCAGCGGTTG GAGCCCCTGT ACAATCGCTA CGAGGAGCCC 
AACGCCTGGA GGATATCGCG TGCGTTCAGG CGACGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014814670.1
CDS	28..3606
Translation	MADLGTLEELAKLEYLSLVSKVCTELDNHLGINDKDLAEFVISLAEKNTTFDTFKAVLLKNGAEFTDSLISNLLRLIQTMRPPPKASTSKETVAKPKSEKEKLRELFPALCRPDNPNIRNMLDEDDVKVAADALKELEALMPSADRQGKQRSSDHRAKKKRRSRSRSRDGRRRHRSRSRSRSRTRDKNRGKSRYRSRSRSWSPSRDRRDREKYVEKSNERWRDKHIDRPPPEEPSIGDIYNGKVTSIMQFGCFVQLEGLRKRWEGLVHISELRREGRVANVADVVSKGQRVKVKVLSFTGSKTSLSMKDVDQSTGEDLNPNRRRNLVGETNEEISMRNPDRPSHLSLVNAPEVEDDSLERKRLTRISDPEKWEIKQMIAANVLSKEEFPDFDEETGILPKVDDEEDEDLEIELVEEEPPFLRGHTKQSMDMSPIKIVKNPDGSLSQAAMMQSALAKERRELKQAQREAEMDSIPMGLNTHWVDPLPDVDGRQIAANMRGIGMMPNDIPEWKKHAFGGNKASYGKKTQLSIIEQRESLPIFRLKEQLIQAVHDNQILIVIGETGSGKTTQITQYLAEAGYTSRGKIGCTQPRRVAAMSVAKRVSEEFGCCLGQEVGYTIRFEDCTSPETVIKYMTDGMLLRECLIDPDLTQYAIIMLDEAHERTIHTDVLFGLLKKTVQKRQDMKLIVTSATLDAVKFSQYFYEAPIFTIPGRTYPVEILYTKEPETDYLDASLITVMQIHLTEPPGDILVFLTGQEEIDTACEILYERMKSLGPDVPELIILPVYSALPSEMQTRIFDPAPPGSRKVVIATNIAETSLTIDGIYYVVDPGFVKQKVYNSKTGIDQLVVTPISQAQAKQRAGRAGRTGPGKCYRLYTERAYRDEMLTTNVPEIQRTNLASTVLSLKAMGINDLLSFDFMDAPPMETLITAMEQLYTLGALDDEGLLTRLGRRMAEFPLEPMLCKMLIMSVHLGCSEEMLTIVSMLSVQNVFYRPKDKQALADQKKAKFHQTEGDHLTLLAVYNSWKNNKFSNPWCYENFIQARSLRRAQDIRKQMLGIMDRHKLDVVSCGKATVRVQKAICSGFFRNAAKKDPQEGYRTLIDQQVVYIHPSSALFNRQPEWVVYHELVLTTKEYMREVTTIDPRWLVEFAPAFFKVSDPTKLSKQKKQQRLEPLYNRYEEPNAWRISRAFRRR

Target ORF information:

RefSeq Version	XM_014959184.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax DEAH (Asp-Glu-Ala-His) box polypeptide 8 (DHX8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014959184.1

ORF Insert Sequence:

ATGGCGGACC TGGGGACGCT GGAGGAGCTC GCGAAGCTCG AGTACCTGTC GCTGGTCTCC 
AAGGTGTGCA CCGAGCTCGA TAATCACCTG GGAATCAACG ACAAGGACCT GGCCGAGTTC 
GTGATAAGTC TTGCTGAGAA AAATACTACA TTTGACACAT TTAAAGCAGT TCTTCTAAAG 
AATGGGGCCG AGTTCACTGA TTCCCTTATT AGTAATTTGT TGCGTCTCAT ACAGACGATG 
CGGCCTCCAC CGAAAGCATC TACGAGCAAA GAGACAGTTG CCAAACCCAA ATCGGAGAAG 
GAAAAGTTGA GGGAATTGTT TCCAGCCCTT TGCAGGCCAG ACAATCCCAA CATCAGGAAT 
ATGTTGGATG AAGATGATGT CAAAGTGGCA GCTGATGCAC TTAAAGAACT CGAAGCTCTG 
ATGCCAAGTG CGGACAGGCA AGGCAAGCAG CGGAGCAGCG ATCACCGGGC GAAGAAAAAG 
AGGAGGAGTC GAAGTCGTAG CAGGGACGGG AGACGCCGGC ACAGATCTCG CTCGAGGTCT 
CGTTCTAGGA CTCGGGATAA GAACAGGGGA AAATCCAGAT ACAGGTCAAG GAGCAGAAGT 
TGGAGTCCCA GTAGGGACCG TAGGGATCGA GAGAAGTATG TTGAGAAGAG CAACGAGAGA 
TGGAGAGACA AACACATTGA CCGTCCACCA CCTGAGGAGC CTTCCATTGG AGACATTTAC 
AACGGCAAAG TCACCAGTAT AATGCAGTTT GGATGCTTTG TGCAGCTGGA AGGACTAAGG 
AAGCGTTGGG AGGGCTTGGT CCACATCTCA GAGCTTCGAA GAGAAGGACG GGTTGCCAAT 
GTTGCTGATG TTGTGAGCAA AGGACAGAGA GTAAAAGTCA AAGTGTTATC CTTTACTGGA 
TCCAAAACCA GCCTGAGCAT GAAGGATGTT GATCAAAGCA CTGGGGAAGA CTTGAACCCA 
AACCGGAGGA GAAACCTGGT TGGAGAAACT AATGAAGAAA TCTCGATGAG GAACCCAGAC 
AGACCCAGTC ACCTGTCCCT GGTGAACGCT CCGGAGGTGG AGGATGACAG CCTGGAACGG 
AAACGCCTTA CCCGAATTTC AGACCCAGAG AAGTGGGAGA TTAAACAGAT GATTGCAGCT 
AATGTGCTTT CCAAAGAAGA GTTTCCTGAC TTTGATGAGG AGACTGGGAT TCTTCCTAAA 
GTTGATGATG AAGAAGATGA GGACCTTGAA ATTGAGTTAG TTGAAGAAGA GCCACCCTTC 
CTTCGAGGTC ATACTAAACA GAGCATGGAT ATGAGTCCCA TCAAAATAGT CAAGAATCCA 
GATGGTTCCT TGTCCCAGGC TGCAATGATG CAGAGTGCTT TAGCTAAAGA AAGACGAGAA 
CTTAAACAAG CCCAGAGAGA GGCAGAGATG GATTCTATCC CCATGGGACT GAACACACAC 
TGGGTGGATC CTCTCCCTGA CGTGGATGGA AGACAAATTG CCGCAAACAT GCGGGGTATT 
GGGATGATGC CCAATGATAT CCCAGAGTGG AAGAAACATG CATTTGGTGG CAACAAAGCT 
TCTTATGGTA AGAAGACCCA GCTTTCCATC ATTGAGCAGA GAGAGAGTCT CCCAATCTTC 
AGACTGAAGG AGCAGCTGAT ACAAGCTGTG CATGACAACC AGATTCTGAT TGTTATTGGA 
GAGACAGGAT CTGGGAAAAC AACACAGATT ACCCAGTACC TGGCTGAGGC AGGTTACACA 
TCGAGAGGAA AGATTGGATG TACTCAGCCT CGCAGAGTGG CTGCAATGTC TGTTGCAAAG 
AGGGTGTCCG AGGAGTTTGG TTGCTGCTTG GGACAGGAGG TTGGCTACAC CATTCGATTT 
GAAGACTGCA CCAGCCCTGA AACTGTTATC AAATACATGA CAGATGGTAT GTTACTGAGG 
GAGTGCTTGA TAGATCCTGA CCTGACTCAA TATGCCATCA TCATGCTGGA TGAAGCCCAT 
GAGAGGACCA TACATACAGA TGTGCTCTTT GGGCTACTGA AAAAGACAGT GCAGAAACGG 
CAGGACATGA AGCTGATAGT GACCTCAGCC ACACTGGATG CTGTGAAATT TTCTCAGTAT 
TTCTACGAAG CACCAATCTT CACAATTCCT GGCAGAACAT ACCCAGTAGA AATTCTGTAC 
ACAAAAGAGC CAGAAACAGA TTATTTGGAT GCCAGTCTCA TTACAGTAAT GCAAATCCAC 
TTAACAGAGC CACCAGGTGA CATTCTGGTG TTTCTGACTG GTCAGGAAGA GATTGACACA 
GCCTGTGAAA TCCTCTATGA GAGGATGAAA TCTCTAGGAC CTGATGTTCC AGAGTTAATT 
ATCCTACCTG TATATTCAGC TTTACCCAGT GAAATGCAAA CCAGGATTTT TGACCCAGCC 
CCACCAGGAA GCAGAAAGGT TGTCATCGCT ACTAACATTG CTGAGACATC TTTGACTATT 
GATGGGATAT ATTACGTAGT GGATCCTGGC TTTGTGAAGC AGAAAGTTTA TAACTCGAAG 
ACTGGAATTG ACCAGCTGGT TGTTACACCA ATTTCACAGG CTCAAGCGAA GCAACGAGCA 
GGAAGGGCTG GGAGAACAGG ACCAGGAAAA TGCTATAGGT TATATACAGA ACGTGCTTAT 
CGAGATGAAA TGCTAACCAC CAATGTGCCT GAAATCCAGA GGACAAATCT GGCCAGTACA 
GTACTTTCCC TGAAGGCCAT GGGCATCAAT GATTTGCTCT CCTTTGACTT TATGGATGCT 
CCCCCGATGG AAACTCTTAT AACAGCCATG GAGCAGCTCT ACACCCTGGG AGCTCTGGAC 
GACGAGGGAC TGCTCACGCG ACTGGGGCGC AGGATGGCGG AATTCCCCTT GGAGCCTATG 
TTGTGTAAGA TGTTGATCAT GTCTGTCCAT CTGGGATGCA GTGAGGAAAT GCTGACAATA 
GTATCCATGC TGTCTGTACA GAATGTGTTC TACAGGCCAA AGGATAAACA AGCACTTGCT 
GATCAAAAGA AAGCCAAGTT CCATCAGACA GAAGGGGACC ACCTCACTCT GCTGGCTGTG 
TACAATTCCT GGAAGAATAA TAAGTTTTCA AATCCCTGGT GCTATGAAAA TTTTATCCAG 
GCCCGATCCT TACGCAGGGC ACAGGACATC CGCAAGCAGA TGCTGGGCAT TATGGACAGG 
CACAAGCTGG ATGTGGTATC CTGTGGGAAG GCAACAGTTC GAGTCCAGAA GGCCATCTGC 
AGTGGTTTCT TCAGAAATGC AGCAAAGAAG GATCCGCAGG AAGGTTATCG GACGCTCATT 
GACCAACAAG TAGTTTATAT CCACCCGTCC AGCGCGCTCT TCAACAGGCA GCCAGAATGG 
GTGGTGTATC ATGAACTGGT GCTGACCACC AAGGAATATA TGCGGGAAGT GACAACTATT 
GATCCTCGCT GGCTGGTTGA ATTTGCACCA GCTTTCTTCA AGGTTTCTGA TCCAACCAAG 
CTGAGCAAAC AGAAGAAGCA GCAGCGGTTG GAGCCCCTGT ACAATCGCTA CGAGGAGCCC 
AACGCCTGGA GGATATCGCG TGCGTTCAGG CGACGATGA