RASGRF2 cDNA ORF clone, Calidris pugnax(ruff)

Gene
Clones
gRNAs

Summary

Gene Symbol	RASGRF2
Entrez Gene ID	106898931
Full Name	Ras protein specific guanine nucleotide releasing factor 2
Gene Type	protein-coding
Organism	Calidris pugnax(ruff)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014960533.1	XP_014816019.1	ras-specific guanine nucleotide-releasing factor 2 isoform X1
XM_014960534.1	XP_014816020.1	ras-specific guanine nucleotide-releasing factor 2 isoform X2

Pathways from BioSystems
Gene Ontology

The following RASGRF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RASGRF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCa78608	XM_014960533.1 Latest version!	Calidris pugnax Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCa78609	XM_014960534.1 Latest version!	Calidris pugnax Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RASGRF2 ( XM_014960533.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014816019.1 Calidris pugnax Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCa78608
Clone ID Related Accession (Same CDS sequence)	XM_014960533.1
Accession Version	XM_014960533.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3720bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	ras-specific guanine nucleotide-releasing factor 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091265.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAGA GCGTGCGGTA TAACGAGGGG CACGCCCTGT ACCTGGCCCT CTTGGCCCGC 
AAGGAAGGCA CCAAGCGGGG CTACCTGAGC AAGAAGACGG CGGAGACCAA CCGCTGGCAC 
GAAAAGTGGT TCGCCCTCTA CCAAAACGTC CTCTTCTACT TCGAAGGGGA GCAGAGCGCC 
CGCCCCGCCG GCATGTACAT GCTGGAGGGC TGCAACTGCG AGAGGGTGCC GGCTCCCAAA 
AGCTCCGCCG CCGGCAGCGC CAAGGATGCT GCGCTGGAGA AGCAGCATTA CTTTACTGTT 
CTTTTTGGCC ACGAAGGGCA GAAGCCACTG GAGCTCCGTT GTGAGGATGA AGCTGATGGA 
GATGAATGGG TAGAAGCTAT TCACCAAGCT AGTTATTCAG ACATTTTGAT TGAAAGGGAG 
GTGTTAATGC AGAAGTACAT TCATCTCGTC CAGATTGTAG AGACTGAAAA GATTGCAGCC 
AATCAGCTTC GACATCAGCT TGAAGATCAA GACACAGAAA TTGAGAGACT TAAATCTGAG 
ATTATAGCTC TCAATAAAAC AAAAGAAAGA ATGCGACCTT ATCAAGGCAA CCAGGAAGAT 
GAGGATCCAG ATATTAAAAA AATTAAAAAG GTCCAGAGCT TTATGCGGGG ATGGTTATGT 
AGAAGAAAAT GGAAAACTAT CGTCCAAGAT TATATTTGTT CTCCCCATGC AGAAAGCATG 
AGAAAAAGGA ATCAGATAGT TTTTAACATG GTGGAGGCTG AATCTGAATA TGTGCATCAA 
CTTTATGTTC TTGTGAACTG TTTTCTGAGG CCCTTAAGAA TGGCTGCAAG TTCAAAGAAG 
CCACCTATCA GTCATGATGA TGTTAGTAGC ATTTTCCTCA ACAGTGAAAC AATCATGTTT 
CTTCATGAGA TATTTCACCA AGGCTTAAAA GCCAGAATAG CTAATTGGCC TACCTTAATC 
CTAGCTGACT TATTTGACAT TCTGCTGCCA ATGCTGAACA TATATCAAGA ATTTGTTCGG 
AATCATCAAT ATAGTCTACA AGTACTGGCA AACTGTAAGC AAAACAGAGA TTTTGACAAA 
CTCTTGAAGC AATATGAAGC CAACCCAGCA TGTGAAGGGC GAATGCTGGA AACTTTCCTT 
ACTTACCCCA TGTTTCAGAT TCCTAGATAT ATTATAACAC TTCATGAACT TCTGGCTCAC 
ACACCACATG AGCATGTCGA GCGAAAAAGC CTTGAATTTG CTAAATCTAA ACTAGAAGAA 
CTTTCAAGGG TGATGCACGA TGAAGTGAGT GACACAGAAA ACATCCGGAA GAATCTAGCT 
ATAGAAAGAA CAATAGTAGA AGGCTGTGAT ATATTACTAG ATACCAGCCA AACTTTTATA 
CGCCAAGGTT CCCTTATACA AGTCCCTTCA GTTGAGAGGG GAAAACTTAG CAAAGTTCGC 
CTGGGATCAT TATCTTTGAA GAAAGAAGGA GAAAGGCAGT GCTTCTTATT TACAAAACAT 
TTTTTAATTT GTACAAGAAG TTCAGGAGGA AAACTGCATC TTCTCAAGAC AGGAGGTGTT 
CTGTCTTTGA TAGAGTGCAC ACTGATTGAG GAGACAGACG TGAGTGATGA TGATTCAAAA 
GGTTCCGGAC AAGTGTTTGG ACACCTTGAT TTCAAGCTTG TAGTGGAACC CACGGATGCT 
CCTCCTTTTA CTGTTGTCCT TTTAGCGCCA TCACGACAGG AGAAAGCAGC ATGGACAAGT 
GATATAAGTC AGTGCATTGA TAATATAAGG TGCAATGGTT TAATGACCAT AGTGTTTGAA 
GAAAACTCTA AAGTCACGGT GCCACATATG ATCAAATCTG ATGCTCGTCT TCATAGAGAT 
GACATTGATA TCTGCTTCAG CAAAACACTG AATTCCTGCA AAGTACCCCA AATCCGTTAC 
GCGAGCGTCG AGCGCCTTCT GGAGCGGCTG ACGGACTTAC GATTCCTAAG TATTGATTTC 
CTGAATACTT TCTTACATAC TTACCGCATA TTTACTACTG CAGCTGTTGT TCTGGAAAAG 
CTCTCAGACA TATACAGACG GCCCTTCACT TCCATTCCCG TCAGGTCTTT GGAGTTATTC 
TTTGCTACCA GTCAAAACAA CAGAGGAGAG TACCTGGCTG ATGGGAAGTC ACCACACCTC 
TGCCGCAAGT TCTCTTCTCC TCCTCCATTG TCACTCTCCA GGACTTCCTC ACCTGTAAGA 
ACCCGAAAAC TGTCATTGAC CTCACCACTG AATTCAAGGA TTGGTGCCCT TGACCTTTCT 
ACTTCATCTG TGGCAAGCAG TCCTACCTCG ACCTACAGCC CAGCCACCTC CCCACCGCCA 
ACGGGTAGCA AAGTACCACT CGACCTTAGC AAGGGGCCCT CCTCTCCTGA GCAAAGCCCA 
GGCTCCATAG AGGACAGCTT GGAGAACCCT CGCGTTGACC TCTGTAACAA GCTGAGAAGA 
AGTATTCGAA GAGCAGCAGT TCTAGAATCT GTGTCAGTGG ACCGGACAAT TCCTGAAAGC 
ACCTCAGCAG TGGATACTAC AGAGCTTTCC CCATGTAGAT CCCCCTCAAC ACCACGTCAT 
CTCCGCTATC GTCAACCTGG AGTACAAACT GCTGACAACA GCCACTGTTC CGTTTCTCCT 
GCCTCTGCTT TTGCTATTGC TACCGCCGCA GCAGGACACG GGAGCCCACC AGGATTTAAC 
AACTCTGAAC GCATGTGTGA CAAAGAGTTC ATAATACGCA GAGCAGCCAC GAATCGTGTC 
CTAAATGTCT TGCGCCACTG GGTCTCCAAG CATTCTCAGG ATTTTGAGCT GAACAATGAG 
CTGAAAATGA ATGTGTTAAA TTTGCTGGAA GAAGTTTTGA GAGACCCCGA CCTTCTGCCA 
CAAGAAAGGA AAGCTACTGC AAATATACTG AGGGCTCTTT CTCAGGATGA TCAAGATGAT 
ACCCATTTAA AAATAGAGGA TATCATTCAG ATGTCAGACT GTCCGAAACC AGAGTGCTTT 
GAGACACTGT CGGCCATGGA GCTTGCAGAG CAAATAACTC TTTTAGATCA CGTAGTTTTC 
CGAAGCATAC CCTACGAAGA GTTTCTTGGG CAGGGATGGA TGAAAGTGGA TAAAAATGAG 
AGAACACCCT ATATTATGAA AACTAGTCAG CATTTTAATG ATATGAGTAA CCTTGTTGCC 
TCCCAAATCA TGAATTATGC GGATGTCAGC TCCCGAGCCA ACTCGATTGA GAAGTGGGTA 
GCAGTAGCTG ATATTTGTCG ATGTATGCAC AATTATAATG GTGTGTTAGA AATCACGTCA 
GCCTTGAACA GAAGTGCAAT CTACAGACTT AAGAAAACTT GGGGTAAAGT ATCCAAACAG 
ACAAAAGCTC TCATGGACAA GCTTCAGAAG ACTGTGTCAT CTGAAGGAAG ATTTAAAAAC 
CTTAGAGAAA CACTCAAAAA TTGTAACCCA CCTGCTGTTC CCTACCTTGG AATGTACCTG 
ACGGACCTGG CGTTTATTGA GGAAGGGACA CCAAACTTCA CTGAGGAAGG CCTTGTCAAT 
TTTTCCAAAA TGAGAATGAT CTCACATATT ATCAGGGAAA TACGCCAGTT CCAGCAGACC 
TCCTACCGCA TAGAGCATCA GCAGAAGGTC ACTCACTATT TACTTGACAA GACACTCATC 
ATAGACGAAG ACACTTTGTA TGAGCTTTCC CTAAAGATTG AACCCAGACT CCCTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014816019.1
CDS	258..3977
Translation	MQKSVRYNEGHALYLALLARKEGTKRGYLSKKTAETNRWHEKWFALYQNVLFYFEGEQSARPAGMYMLEGCNCERVPAPKSSAAGSAKDAALEKQHYFTVLFGHEGQKPLELRCEDEADGDEWVEAIHQASYSDILIEREVLMQKYIHLVQIVETEKIAANQLRHQLEDQDTEIERLKSEIIALNKTKERMRPYQGNQEDEDPDIKKIKKVQSFMRGWLCRRKWKTIVQDYICSPHAESMRKRNQIVFNMVEAESEYVHQLYVLVNCFLRPLRMAASSKKPPISHDDVSSIFLNSETIMFLHEIFHQGLKARIANWPTLILADLFDILLPMLNIYQEFVRNHQYSLQVLANCKQNRDFDKLLKQYEANPACEGRMLETFLTYPMFQIPRYIITLHELLAHTPHEHVERKSLEFAKSKLEELSRVMHDEVSDTENIRKNLAIERTIVEGCDILLDTSQTFIRQGSLIQVPSVERGKLSKVRLGSLSLKKEGERQCFLFTKHFLICTRSSGGKLHLLKTGGVLSLIECTLIEETDVSDDDSKGSGQVFGHLDFKLVVEPTDAPPFTVVLLAPSRQEKAAWTSDISQCIDNIRCNGLMTIVFEENSKVTVPHMIKSDARLHRDDIDICFSKTLNSCKVPQIRYASVERLLERLTDLRFLSIDFLNTFLHTYRIFTTAAVVLEKLSDIYRRPFTSIPVRSLELFFATSQNNRGEYLADGKSPHLCRKFSSPPPLSLSRTSSPVRTRKLSLTSPLNSRIGALDLSTSSVASSPTSTYSPATSPPPTGSKVPLDLSKGPSSPEQSPGSIEDSLENPRVDLCNKLRRSIRRAAVLESVSVDRTIPESTSAVDTTELSPCRSPSTPRHLRYRQPGVQTADNSHCSVSPASAFAIATAAAGHGSPPGFNNSERMCDKEFIIRRAATNRVLNVLRHWVSKHSQDFELNNELKMNVLNLLEEVLRDPDLLPQERKATANILRALSQDDQDDTHLKIEDIIQMSDCPKPECFETLSAMELAEQITLLDHVVFRSIPYEEFLGQGWMKVDKNERTPYIMKTSQHFNDMSNLVASQIMNYADVSSRANSIEKWVAVADICRCMHNYNGVLEITSALNRSAIYRLKKTWGKVSKQTKALMDKLQKTVSSEGRFKNLRETLKNCNPPAVPYLGMYLTDLAFIEEGTPNFTEEGLVNFSKMRMISHIIREIRQFQQTSYRIEHQQKVTHYLLDKTLIIDEDTLYELSLKIEPRLPA

Target ORF information:

RefSeq Version	XM_014960533.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014960533.1

ORF Insert Sequence:

ATGCAGAAGA GCGTGCGGTA TAACGAGGGG CACGCCCTGT ACCTGGCCCT CTTGGCCCGC 
AAGGAAGGCA CCAAGCGGGG CTACCTGAGC AAGAAGACGG CGGAGACCAA CCGCTGGCAC 
GAAAAGTGGT TCGCCCTCTA CCAAAACGTC CTCTTCTACT TCGAAGGGGA GCAGAGCGCC 
CGCCCCGCCG GCATGTACAT GCTGGAGGGC TGCAACTGCG AGAGGGTGCC GGCTCCCAAA 
AGCTCCGCCG CCGGCAGCGC CAAGGATGCT GCGCTGGAGA AGCAGCATTA CTTTACTGTT 
CTTTTTGGCC ACGAAGGGCA GAAGCCACTG GAGCTCCGTT GTGAGGATGA AGCTGATGGA 
GATGAATGGG TAGAAGCTAT TCACCAAGCT AGTTATTCAG ACATTTTGAT TGAAAGGGAG 
GTGTTAATGC AGAAGTACAT TCATCTCGTC CAGATTGTAG AGACTGAAAA GATTGCAGCC 
AATCAGCTTC GACATCAGCT TGAAGATCAA GACACAGAAA TTGAGAGACT TAAATCTGAG 
ATTATAGCTC TCAATAAAAC AAAAGAAAGA ATGCGACCTT ATCAAGGCAA CCAGGAAGAT 
GAGGATCCAG ATATTAAAAA AATTAAAAAG GTCCAGAGCT TTATGCGGGG ATGGTTATGT 
AGAAGAAAAT GGAAAACTAT CGTCCAAGAT TATATTTGTT CTCCCCATGC AGAAAGCATG 
AGAAAAAGGA ATCAGATAGT TTTTAACATG GTGGAGGCTG AATCTGAATA TGTGCATCAA 
CTTTATGTTC TTGTGAACTG TTTTCTGAGG CCCTTAAGAA TGGCTGCAAG TTCAAAGAAG 
CCACCTATCA GTCATGATGA TGTTAGTAGC ATTTTCCTCA ACAGTGAAAC AATCATGTTT 
CTTCATGAGA TATTTCACCA AGGCTTAAAA GCCAGAATAG CTAATTGGCC TACCTTAATC 
CTAGCTGACT TATTTGACAT TCTGCTGCCA ATGCTGAACA TATATCAAGA ATTTGTTCGG 
AATCATCAAT ATAGTCTACA AGTACTGGCA AACTGTAAGC AAAACAGAGA TTTTGACAAA 
CTCTTGAAGC AATATGAAGC CAACCCAGCA TGTGAAGGGC GAATGCTGGA AACTTTCCTT 
ACTTACCCCA TGTTTCAGAT TCCTAGATAT ATTATAACAC TTCATGAACT TCTGGCTCAC 
ACACCACATG AGCATGTCGA GCGAAAAAGC CTTGAATTTG CTAAATCTAA ACTAGAAGAA 
CTTTCAAGGG TGATGCACGA TGAAGTGAGT GACACAGAAA ACATCCGGAA GAATCTAGCT 
ATAGAAAGAA CAATAGTAGA AGGCTGTGAT ATATTACTAG ATACCAGCCA AACTTTTATA 
CGCCAAGGTT CCCTTATACA AGTCCCTTCA GTTGAGAGGG GAAAACTTAG CAAAGTTCGC 
CTGGGATCAT TATCTTTGAA GAAAGAAGGA GAAAGGCAGT GCTTCTTATT TACAAAACAT 
TTTTTAATTT GTACAAGAAG TTCAGGAGGA AAACTGCATC TTCTCAAGAC AGGAGGTGTT 
CTGTCTTTGA TAGAGTGCAC ACTGATTGAG GAGACAGACG TGAGTGATGA TGATTCAAAA 
GGTTCCGGAC AAGTGTTTGG ACACCTTGAT TTCAAGCTTG TAGTGGAACC CACGGATGCT 
CCTCCTTTTA CTGTTGTCCT TTTAGCGCCA TCACGACAGG AGAAAGCAGC ATGGACAAGT 
GATATAAGTC AGTGCATTGA TAATATAAGG TGCAATGGTT TAATGACCAT AGTGTTTGAA 
GAAAACTCTA AAGTCACGGT GCCACATATG ATCAAATCTG ATGCTCGTCT TCATAGAGAT 
GACATTGATA TCTGCTTCAG CAAAACACTG AATTCCTGCA AAGTACCCCA AATCCGTTAC 
GCGAGCGTCG AGCGCCTTCT GGAGCGGCTG ACGGACTTAC GATTCCTAAG TATTGATTTC 
CTGAATACTT TCTTACATAC TTACCGCATA TTTACTACTG CAGCTGTTGT TCTGGAAAAG 
CTCTCAGACA TATACAGACG GCCCTTCACT TCCATTCCCG TCAGGTCTTT GGAGTTATTC 
TTTGCTACCA GTCAAAACAA CAGAGGAGAG TACCTGGCTG ATGGGAAGTC ACCACACCTC 
TGCCGCAAGT TCTCTTCTCC TCCTCCATTG TCACTCTCCA GGACTTCCTC ACCTGTAAGA 
ACCCGAAAAC TGTCATTGAC CTCACCACTG AATTCAAGGA TTGGTGCCCT TGACCTTTCT 
ACTTCATCTG TGGCAAGCAG TCCTACCTCG ACCTACAGCC CAGCCACCTC CCCACCGCCA 
ACGGGTAGCA AAGTACCACT CGACCTTAGC AAGGGGCCCT CCTCTCCTGA GCAAAGCCCA 
GGCTCCATAG AGGACAGCTT GGAGAACCCT CGCGTTGACC TCTGTAACAA GCTGAGAAGA 
AGTATTCGAA GAGCAGCAGT TCTAGAATCT GTGTCAGTGG ACCGGACAAT TCCTGAAAGC 
ACCTCAGCAG TGGATACTAC AGAGCTTTCC CCATGTAGAT CCCCCTCAAC ACCACGTCAT 
CTCCGCTATC GTCAACCTGG AGTACAAACT GCTGACAACA GCCACTGTTC CGTTTCTCCT 
GCCTCTGCTT TTGCTATTGC TACCGCCGCA GCAGGACACG GGAGCCCACC AGGATTTAAC 
AACTCTGAAC GCATGTGTGA CAAAGAGTTC ATAATACGCA GAGCAGCCAC GAATCGTGTC 
CTAAATGTCT TGCGCCACTG GGTCTCCAAG CATTCTCAGG ATTTTGAGCT GAACAATGAG 
CTGAAAATGA ATGTGTTAAA TTTGCTGGAA GAAGTTTTGA GAGACCCCGA CCTTCTGCCA 
CAAGAAAGGA AAGCTACTGC AAATATACTG AGGGCTCTTT CTCAGGATGA TCAAGATGAT 
ACCCATTTAA AAATAGAGGA TATCATTCAG ATGTCAGACT GTCCGAAACC AGAGTGCTTT 
GAGACACTGT CGGCCATGGA GCTTGCAGAG CAAATAACTC TTTTAGATCA CGTAGTTTTC 
CGAAGCATAC CCTACGAAGA GTTTCTTGGG CAGGGATGGA TGAAAGTGGA TAAAAATGAG 
AGAACACCCT ATATTATGAA AACTAGTCAG CATTTTAATG ATATGAGTAA CCTTGTTGCC 
TCCCAAATCA TGAATTATGC GGATGTCAGC TCCCGAGCCA ACTCGATTGA GAAGTGGGTA 
GCAGTAGCTG ATATTTGTCG ATGTATGCAC AATTATAATG GTGTGTTAGA AATCACGTCA 
GCCTTGAACA GAAGTGCAAT CTACAGACTT AAGAAAACTT GGGGTAAAGT ATCCAAACAG 
ACAAAAGCTC TCATGGACAA GCTTCAGAAG ACTGTGTCAT CTGAAGGAAG ATTTAAAAAC 
CTTAGAGAAA CACTCAAAAA TTGTAACCCA CCTGCTGTTC CCTACCTTGG AATGTACCTG 
ACGGACCTGG CGTTTATTGA GGAAGGGACA CCAAACTTCA CTGAGGAAGG CCTTGTCAAT 
TTTTCCAAAA TGAGAATGAT CTCACATATT ATCAGGGAAA TACGCCAGTT CCAGCAGACC 
TCCTACCGCA TAGAGCATCA GCAGAAGGTC ACTCACTATT TACTTGACAA GACACTCATC 
ATAGACGAAG ACACTTTGTA TGAGCTTTCC CTAAAGATTG AACCCAGACT CCCTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGRF2 ( XM_014960534.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014816020.1 Calidris pugnax Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCa78609
Clone ID Related Accession (Same CDS sequence)	XM_014960534.1
Accession Version	XM_014960534.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	ras-specific guanine nucleotide-releasing factor 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091265.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAGA GCGTGCGGTA TAACGAGGGG CACGCCCTGT ACCTGGCCCT CTTGGCCCGC 
AAGGAAGGCA CCAAGCGGGG CTACCTGAGC AAGAAGACGG CGGAGACCAA CCGCTGGCAC 
GAAAAGTGGT TCGCCCTCTA CCAAAACGTC CTCTTCTACT TCGAAGGGGA GCAGAGCGCC 
CGCCCCGCCG GCATGTACAT GCTGGAGGGC TGCAACTGCG AGAGGGTGCC GGCTCCCAAA 
AGCTCCGCCG CCGGCAGCGC CAAGGATGCT GCGCTGGAGA AGCAGCATTA CTTTACTGTT 
CTTTTTGGCC ACGAAGGGCA GAAGCCACTG GAGCTCCGTT GTGAGGATGA AGCTGATGGA 
GATGAATGGG TAGAAGCTAT TCACCAAGCT AGTTATTCAG ACATTTTGAT TGAAAGGGAG 
GTGTTAATGC AGAAGTACAT TCATCTCGTC CAGATTGTAG AGACTGAAAA GATTGCAGCC 
AATCAGCTTC GACATCAGCT TGAAGATCAA GACACAGAAA TTGAGAGACT TAAATCTGAG 
ATTATAGCTC TCAATAAAAC AAAAGAAAGA ATGCGACCTT ATCAAGGCAA CCAGGAAGAT 
GAGGATCCAG ATATTAAAAA AATTAAAAAG GTCCAGAGCT TTATGCGGGG ATGGTTATGT 
AGAAGAAAAT GGAAAACTAT CGTCCAAGAT TATATTTGTT CTCCCCATGC AGAAAGCATG 
AGAAAAAGGA ATCAGATAGT TTTTAACATG GTGGAGGCTG AATCTGAATA TGTGCATCAA 
CTTTATGTTC TTGTGAACTG TTTTCTGAGG CCCTTAAGAA TGGCTGCAAG TTCAAAGAAG 
CCACCTATCA GTCATGATGA TGTTAGTAGC ATTTTCCTCA ACAGTGAAAC AATCATGTTT 
CTTCATGAGA TATTTCACCA AGGCTTAAAA GCCAGAATAG CTAATTGGCC TACCTTAATC 
CTAGCTGACT TATTTGACAT TCTGCTGCCA ATGCTGAACA TATATCAAGA ATTTGTTCGG 
AATCATCAAT ATAGTCTACA AGTACTGGCA AACTGTAAGC AAAACAGAGA TTTTGACAAA 
CTCTTGAAGC AATATGAAGC CAACCCAGCA TGTGAAGGGC GAATGCTGGA AACTTTCCTT 
ACTTACCCCA TGTTTCAGAT TCCTAGATAT ATTATAACAC TTCATGAACT TCTGGCTCAC 
ACACCACATG AGCATGTCGA GCGAAAAAGC CTTGAATTTG CTAAATCTAA ACTAGAAGAA 
CTTTCAAGGG TGATGCACGA TGAAGTGAGT GACACAGAAA ACATCCGGAA GAATCTAGCT 
ATAGAAAGAA CAATAGTAGA AGGCTGTGAT ATATTACTAG ATACCAGCCA AACTTTTATA 
CGCCAAGGTT CCCTTATACA AGTCCCTTCA GTTGAGAGGG GAAAACTTAG CAAAGTTCGC 
CTGGGATCAT TATCTTTGAA GAAAGAAGGA GAAAGGCAGT GCTTCTTATT TACAAAACAT 
TTTTTAATTT GTACAAGAAG TTCAGGAGGA AAACTGCATC TTCTCAAGAC AGGAGGTGTT 
CTGTCTTTGA TAGAGTGCAC ACTGATTGAG GAGACAGACG TGAGTGATGA TGATTCAAAA 
GGTTCCGGAC AAGTGTTTGG ACACCTTGAT TTCAAGCTTG TAGTGGAACC CACGGATGCT 
CCTCCTTTTA CTGTTGTCCT TTTAGCGCCA TCACGACAGG AGAAAGCAGC ATGGACAAGT 
GATATAAGTC AGTGCATTGA TAATATAAGG TGCAATGGTT TAATGACCAT AGTGTTTGAA 
GAAAACTCTA AAGTCACGGT GCCACATATG ATCAAATCTG ATGCTCGTCT TCATAGAGAT 
GACATTGATA TCTGCTTCAG CAAAACACTG AATTCCTGCA AAGTACCCCA AATCCGTTAC 
GCGAGCGTCG AGCGCCTTCT GGAGCGGCTG ACGGACTTAC GATTCCTAAG TATTGATTTC 
CTGAATACTT TCTTACATAC TTACCGCATA TTTACTACTG CAGCTGTTGT TCTGGAAAAG 
CTCTCAGACA TATACAGACG GCCCTTCACT TCCATTCCCG TCAGGTCTTT GGAGTTATTC 
TTTGCTACCA GTCAAAACAA CAGAGGAGAG TACCTGGCTG ATGGGAAGTC ACCACACCTC 
TGCCGCAAGT TCTCTTCTCC TCCTCCATTG TCACTCTCCA GGACTTCCTC ACCTGTAAGA 
ACCCGAAAAC TGTCATTGAC CTCACCACTG AATTCAAGGA TTGGTGCCCT TGACCTTTCT 
ACTTCATCTG TGGCAAGCAG TCCTACCTCG ACCTACAGCC CAGCCACCTC CCCACCGCCA 
ACGGGTAGCA AAGTACCACT CGACCTTAGC AAGGGGCCCT CCTCTCCTGA GCAAAGCCCA 
GGCTCCATAG AGGACAGCTT GGAGAACCCT CGCGTTGACC TCTGTAACAA GCTGAGAAGA 
AGTATTCGAA GAGCAGTTCT AGAATCTGTG TCAGTGGACC GGACAATTCC TGAAAGCACC 
TCAGCAGTGG ATACTACAGA GCTTTCCCCA TGTAGATCCC CCTCAACACC ACGTCATCTC 
CGCTATCGTC AACCTGGAGT ACAAACTGCT GACAACAGCC ACTGTTCCGT TTCTCCTGCC 
TCTGCTTTTG CTATTGCTAC CGCCGCAGCA GGACACGGGA GCCCACCAGG ATTTAACAAC 
TCTGAACGCA TGTGTGACAA AGAGTTCATA ATACGCAGAG CAGCCACGAA TCGTGTCCTA 
AATGTCTTGC GCCACTGGGT CTCCAAGCAT TCTCAGGATT TTGAGCTGAA CAATGAGCTG 
AAAATGAATG TGTTAAATTT GCTGGAAGAA GTTTTGAGAG ACCCCGACCT TCTGCCACAA 
GAAAGGAAAG CTACTGCAAA TATACTGAGG GCTCTTTCTC AGGATGATCA AGATGATACC 
CATTTAAAAA TAGAGGATAT CATTCAGATG TCAGACTGTC CGAAACCAGA GTGCTTTGAG 
ACACTGTCGG CCATGGAGCT TGCAGAGCAA ATAACTCTTT TAGATCACGT AGTTTTCCGA 
AGCATACCCT ACGAAGAGTT TCTTGGGCAG GGATGGATGA AAGTGGATAA AAATGAGAGA 
ACACCCTATA TTATGAAAAC TAGTCAGCAT TTTAATGATA TGAGTAACCT TGTTGCCTCC 
CAAATCATGA ATTATGCGGA TGTCAGCTCC CGAGCCAACT CGATTGAGAA GTGGGTAGCA 
GTAGCTGATA TTTGTCGATG TATGCACAAT TATAATGGTG TGTTAGAAAT CACGTCAGCC 
TTGAACAGAA GTGCAATCTA CAGACTTAAG AAAACTTGGG GTAAAGTATC CAAACAGACA 
AAAGCTCTCA TGGACAAGCT TCAGAAGACT GTGTCATCTG AAGGAAGATT TAAAAACCTT 
AGAGAAACAC TCAAAAATTG TAACCCACCT GCTGTTCCCT ACCTTGGAAT GTACCTGACG 
GACCTGGCGT TTATTGAGGA AGGGACACCA AACTTCACTG AGGAAGGCCT TGTCAATTTT 
TCCAAAATGA GAATGATCTC ACATATTATC AGGGAAATAC GCCAGTTCCA GCAGACCTCC 
TACCGCATAG AGCATCAGCA GAAGGTCACT CACTATTTAC TTGACAAGAC ACTCATCATA 
GACGAAGACA CTTTGTATGA GCTTTCCCTA AAGATTGAAC CCAGACTCCC TGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014816020.1
CDS	258..3974
Translation	MQKSVRYNEGHALYLALLARKEGTKRGYLSKKTAETNRWHEKWFALYQNVLFYFEGEQSARPAGMYMLEGCNCERVPAPKSSAAGSAKDAALEKQHYFTVLFGHEGQKPLELRCEDEADGDEWVEAIHQASYSDILIEREVLMQKYIHLVQIVETEKIAANQLRHQLEDQDTEIERLKSEIIALNKTKERMRPYQGNQEDEDPDIKKIKKVQSFMRGWLCRRKWKTIVQDYICSPHAESMRKRNQIVFNMVEAESEYVHQLYVLVNCFLRPLRMAASSKKPPISHDDVSSIFLNSETIMFLHEIFHQGLKARIANWPTLILADLFDILLPMLNIYQEFVRNHQYSLQVLANCKQNRDFDKLLKQYEANPACEGRMLETFLTYPMFQIPRYIITLHELLAHTPHEHVERKSLEFAKSKLEELSRVMHDEVSDTENIRKNLAIERTIVEGCDILLDTSQTFIRQGSLIQVPSVERGKLSKVRLGSLSLKKEGERQCFLFTKHFLICTRSSGGKLHLLKTGGVLSLIECTLIEETDVSDDDSKGSGQVFGHLDFKLVVEPTDAPPFTVVLLAPSRQEKAAWTSDISQCIDNIRCNGLMTIVFEENSKVTVPHMIKSDARLHRDDIDICFSKTLNSCKVPQIRYASVERLLERLTDLRFLSIDFLNTFLHTYRIFTTAAVVLEKLSDIYRRPFTSIPVRSLELFFATSQNNRGEYLADGKSPHLCRKFSSPPPLSLSRTSSPVRTRKLSLTSPLNSRIGALDLSTSSVASSPTSTYSPATSPPPTGSKVPLDLSKGPSSPEQSPGSIEDSLENPRVDLCNKLRRSIRRAVLESVSVDRTIPESTSAVDTTELSPCRSPSTPRHLRYRQPGVQTADNSHCSVSPASAFAIATAAAGHGSPPGFNNSERMCDKEFIIRRAATNRVLNVLRHWVSKHSQDFELNNELKMNVLNLLEEVLRDPDLLPQERKATANILRALSQDDQDDTHLKIEDIIQMSDCPKPECFETLSAMELAEQITLLDHVVFRSIPYEEFLGQGWMKVDKNERTPYIMKTSQHFNDMSNLVASQIMNYADVSSRANSIEKWVAVADICRCMHNYNGVLEITSALNRSAIYRLKKTWGKVSKQTKALMDKLQKTVSSEGRFKNLRETLKNCNPPAVPYLGMYLTDLAFIEEGTPNFTEEGLVNFSKMRMISHIIREIRQFQQTSYRIEHQQKVTHYLLDKTLIIDEDTLYELSLKIEPRLPA

Target ORF information:

RefSeq Version	XM_014960534.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014960534.1

ORF Insert Sequence:

ATGCAGAAGA GCGTGCGGTA TAACGAGGGG CACGCCCTGT ACCTGGCCCT CTTGGCCCGC 
AAGGAAGGCA CCAAGCGGGG CTACCTGAGC AAGAAGACGG CGGAGACCAA CCGCTGGCAC 
GAAAAGTGGT TCGCCCTCTA CCAAAACGTC CTCTTCTACT TCGAAGGGGA GCAGAGCGCC 
CGCCCCGCCG GCATGTACAT GCTGGAGGGC TGCAACTGCG AGAGGGTGCC GGCTCCCAAA 
AGCTCCGCCG CCGGCAGCGC CAAGGATGCT GCGCTGGAGA AGCAGCATTA CTTTACTGTT 
CTTTTTGGCC ACGAAGGGCA GAAGCCACTG GAGCTCCGTT GTGAGGATGA AGCTGATGGA 
GATGAATGGG TAGAAGCTAT TCACCAAGCT AGTTATTCAG ACATTTTGAT TGAAAGGGAG 
GTGTTAATGC AGAAGTACAT TCATCTCGTC CAGATTGTAG AGACTGAAAA GATTGCAGCC 
AATCAGCTTC GACATCAGCT TGAAGATCAA GACACAGAAA TTGAGAGACT TAAATCTGAG 
ATTATAGCTC TCAATAAAAC AAAAGAAAGA ATGCGACCTT ATCAAGGCAA CCAGGAAGAT 
GAGGATCCAG ATATTAAAAA AATTAAAAAG GTCCAGAGCT TTATGCGGGG ATGGTTATGT 
AGAAGAAAAT GGAAAACTAT CGTCCAAGAT TATATTTGTT CTCCCCATGC AGAAAGCATG 
AGAAAAAGGA ATCAGATAGT TTTTAACATG GTGGAGGCTG AATCTGAATA TGTGCATCAA 
CTTTATGTTC TTGTGAACTG TTTTCTGAGG CCCTTAAGAA TGGCTGCAAG TTCAAAGAAG 
CCACCTATCA GTCATGATGA TGTTAGTAGC ATTTTCCTCA ACAGTGAAAC AATCATGTTT 
CTTCATGAGA TATTTCACCA AGGCTTAAAA GCCAGAATAG CTAATTGGCC TACCTTAATC 
CTAGCTGACT TATTTGACAT TCTGCTGCCA ATGCTGAACA TATATCAAGA ATTTGTTCGG 
AATCATCAAT ATAGTCTACA AGTACTGGCA AACTGTAAGC AAAACAGAGA TTTTGACAAA 
CTCTTGAAGC AATATGAAGC CAACCCAGCA TGTGAAGGGC GAATGCTGGA AACTTTCCTT 
ACTTACCCCA TGTTTCAGAT TCCTAGATAT ATTATAACAC TTCATGAACT TCTGGCTCAC 
ACACCACATG AGCATGTCGA GCGAAAAAGC CTTGAATTTG CTAAATCTAA ACTAGAAGAA 
CTTTCAAGGG TGATGCACGA TGAAGTGAGT GACACAGAAA ACATCCGGAA GAATCTAGCT 
ATAGAAAGAA CAATAGTAGA AGGCTGTGAT ATATTACTAG ATACCAGCCA AACTTTTATA 
CGCCAAGGTT CCCTTATACA AGTCCCTTCA GTTGAGAGGG GAAAACTTAG CAAAGTTCGC 
CTGGGATCAT TATCTTTGAA GAAAGAAGGA GAAAGGCAGT GCTTCTTATT TACAAAACAT 
TTTTTAATTT GTACAAGAAG TTCAGGAGGA AAACTGCATC TTCTCAAGAC AGGAGGTGTT 
CTGTCTTTGA TAGAGTGCAC ACTGATTGAG GAGACAGACG TGAGTGATGA TGATTCAAAA 
GGTTCCGGAC AAGTGTTTGG ACACCTTGAT TTCAAGCTTG TAGTGGAACC CACGGATGCT 
CCTCCTTTTA CTGTTGTCCT TTTAGCGCCA TCACGACAGG AGAAAGCAGC ATGGACAAGT 
GATATAAGTC AGTGCATTGA TAATATAAGG TGCAATGGTT TAATGACCAT AGTGTTTGAA 
GAAAACTCTA AAGTCACGGT GCCACATATG ATCAAATCTG ATGCTCGTCT TCATAGAGAT 
GACATTGATA TCTGCTTCAG CAAAACACTG AATTCCTGCA AAGTACCCCA AATCCGTTAC 
GCGAGCGTCG AGCGCCTTCT GGAGCGGCTG ACGGACTTAC GATTCCTAAG TATTGATTTC 
CTGAATACTT TCTTACATAC TTACCGCATA TTTACTACTG CAGCTGTTGT TCTGGAAAAG 
CTCTCAGACA TATACAGACG GCCCTTCACT TCCATTCCCG TCAGGTCTTT GGAGTTATTC 
TTTGCTACCA GTCAAAACAA CAGAGGAGAG TACCTGGCTG ATGGGAAGTC ACCACACCTC 
TGCCGCAAGT TCTCTTCTCC TCCTCCATTG TCACTCTCCA GGACTTCCTC ACCTGTAAGA 
ACCCGAAAAC TGTCATTGAC CTCACCACTG AATTCAAGGA TTGGTGCCCT TGACCTTTCT 
ACTTCATCTG TGGCAAGCAG TCCTACCTCG ACCTACAGCC CAGCCACCTC CCCACCGCCA 
ACGGGTAGCA AAGTACCACT CGACCTTAGC AAGGGGCCCT CCTCTCCTGA GCAAAGCCCA 
GGCTCCATAG AGGACAGCTT GGAGAACCCT CGCGTTGACC TCTGTAACAA GCTGAGAAGA 
AGTATTCGAA GAGCAGTTCT AGAATCTGTG TCAGTGGACC GGACAATTCC TGAAAGCACC 
TCAGCAGTGG ATACTACAGA GCTTTCCCCA TGTAGATCCC CCTCAACACC ACGTCATCTC 
CGCTATCGTC AACCTGGAGT ACAAACTGCT GACAACAGCC ACTGTTCCGT TTCTCCTGCC 
TCTGCTTTTG CTATTGCTAC CGCCGCAGCA GGACACGGGA GCCCACCAGG ATTTAACAAC 
TCTGAACGCA TGTGTGACAA AGAGTTCATA ATACGCAGAG CAGCCACGAA TCGTGTCCTA 
AATGTCTTGC GCCACTGGGT CTCCAAGCAT TCTCAGGATT TTGAGCTGAA CAATGAGCTG 
AAAATGAATG TGTTAAATTT GCTGGAAGAA GTTTTGAGAG ACCCCGACCT TCTGCCACAA 
GAAAGGAAAG CTACTGCAAA TATACTGAGG GCTCTTTCTC AGGATGATCA AGATGATACC 
CATTTAAAAA TAGAGGATAT CATTCAGATG TCAGACTGTC CGAAACCAGA GTGCTTTGAG 
ACACTGTCGG CCATGGAGCT TGCAGAGCAA ATAACTCTTT TAGATCACGT AGTTTTCCGA 
AGCATACCCT ACGAAGAGTT TCTTGGGCAG GGATGGATGA AAGTGGATAA AAATGAGAGA 
ACACCCTATA TTATGAAAAC TAGTCAGCAT TTTAATGATA TGAGTAACCT TGTTGCCTCC 
CAAATCATGA ATTATGCGGA TGTCAGCTCC CGAGCCAACT CGATTGAGAA GTGGGTAGCA 
GTAGCTGATA TTTGTCGATG TATGCACAAT TATAATGGTG TGTTAGAAAT CACGTCAGCC 
TTGAACAGAA GTGCAATCTA CAGACTTAAG AAAACTTGGG GTAAAGTATC CAAACAGACA 
AAAGCTCTCA TGGACAAGCT TCAGAAGACT GTGTCATCTG AAGGAAGATT TAAAAACCTT 
AGAGAAACAC TCAAAAATTG TAACCCACCT GCTGTTCCCT ACCTTGGAAT GTACCTGACG 
GACCTGGCGT TTATTGAGGA AGGGACACCA AACTTCACTG AGGAAGGCCT TGTCAATTTT 
TCCAAAATGA GAATGATCTC ACATATTATC AGGGAAATAC GCCAGTTCCA GCAGACCTCC 
TACCGCATAG AGCATCAGCA GAAGGTCACT CACTATTTAC TTGACAAGAC ACTCATCATA 
GACGAAGACA CTTTGTATGA GCTTTCCCTA AAGATTGAAC CCAGACTCCC TGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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RASGRF2 cDNA ORF clone, Calidris pugnax(ruff)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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