TMEM116 cDNA ORF clone, Calidris pugnax(ruff)

Gene
Clones
gRNAs

Summary

Gene Symbol	TMEM116
Entrez Gene ID	106899154
Full Name	transmembrane protein 116
Gene Type	protein-coding
Organism	Calidris pugnax(ruff)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014960840.1	XP_014816326.1	transmembrane protein 116

Pathways from BioSystems
Gene Ontology

The following TMEM116 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TMEM116 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCa59730	XM_014960840.1 Latest version!	Calidris pugnax transmembrane protein 116 (TMEM116), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TMEM116 ( XM_014960840.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014816326.1 Calidris pugnax transmembrane protein 116 (TMEM116), mRNA.

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CloneID	OCa59730
Clone ID Related Accession (Same CDS sequence)	XM_014960840.1
Accession Version	XM_014960840.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1089bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	transmembrane protein 116
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090783.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTCC CGGCGGAGGC GGCCCGGCTG GATGGGGGCT GGCAGGAGGT CTACTCGGCT 
CTCCAGTGGA TACAGTTCAC CATGGCCATG CTCAGTGTTA TAGGTTCCAG CTCAATTATT 
GCCTATGCCA TCTTTCAAAA TGTGGTGCGG TCCCCCGAGG TTCGTCCTCT TTTCTACCTG 
AGCCTCTCTG ATCTACTTCT GGGCATGTGC TGGCTTGCTG GAGCGCTTTT CTATAGCACA 
CCGACCTCCA AGCAGGATCT CATCTGCTAT AACCTGCAAG CAACAGGACA AATATTCTAT 
GTGGCCTCTT TCCTATACAC TGCCAACTAC ACCTGGCATC TGTACATGGA CCTCAAGGTG 
AAATACAACC AGAACCTTTT CAGGATGCCC CCCCAGGTTG TAGATTATGC AAGTTGCATT 
GGCCGAATAG CAACGATCTT GTCCAGCCTG ATCCCTTTCC TTCTCATGGT GCCTGTGTTT 
TGCCTGGGCA ACAGCAGTAA TTGCTACCAG AACTTCAGCC AGAAGCACGG ATGTCTCTTG 
ATGCACACGG AAATCGCCAT GCCCACCAAC GAGCCGCAAA CCCTGAGTGG CTCTGTTTGC 
CGTGCGATGC ATTTCTATGG CATTGGGATC TTCCTCATTT CCTTTCTGAT CAGCTTCATA 
GCCATTTTGG TTATACTCAG TCAAGCCCGA GGCTTGTACA AAAGGTTTGT AAACTCCACA 
GGATTCCTGG GGGATCAGCA GTGGGCCATG ATTAAGATGG TAGAACAACG AGTGGTTTTT 
TACCCCATCG CATTCTTCTG CTGCTGGGCC CCAGCTGTCC TCCTTGGAAT ACTGAAGCTG 
ACTGTTTCAA CCAACAGCAA AATCTACATG GCTCTTCTAA TTCTACAGGC TCTGACAGCA 
GCTTCCCAGG GGCTTCTGAA CTGCATCGTC TACGGCTGGA CGCAGCACGT CTTCCTCTCC 
CTCAAACGCA ACGCCTGCCG AGATGTCGAC ACTCAGACTC CTCTTCTACG CTCCCAGAAG 
AAGTTCTACT CCAGCACGCT CCCTGCCAGC CCACCAGATG CAGAGGGCTC CACGTCCACC 
CTGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014816326.1
CDS	51..1139
Translation	MALPAEAARLDGGWQEVYSALQWIQFTMAMLSVIGSSSIIAYAIFQNVVRSPEVRPLFYLSLSDLLLGMCWLAGALFYSTPTSKQDLICYNLQATGQIFYVASFLYTANYTWHLYMDLKVKYNQNLFRMPPQVVDYASCIGRIATILSSLIPFLLMVPVFCLGNSSNCYQNFSQKHGCLLMHTEIAMPTNEPQTLSGSVCRAMHFYGIGIFLISFLISFIAILVILSQARGLYKRFVNSTGFLGDQQWAMIKMVEQRVVFYPIAFFCCWAPAVLLGILKLTVSTNSKIYMALLILQALTAASQGLLNCIVYGWTQHVFLSLKRNACRDVDTQTPLLRSQKKFYSSTLPASPPDAEGSTSTLL

Target ORF information:

RefSeq Version	XM_014960840.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax transmembrane protein 116 (TMEM116), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014960840.1

ORF Insert Sequence:

ATGGCCCTCC CGGCGGAGGC GGCCCGGCTG GATGGGGGCT GGCAGGAGGT CTACTCGGCT 
CTCCAGTGGA TACAGTTCAC CATGGCCATG CTCAGTGTTA TAGGTTCCAG CTCAATTATT 
GCCTATGCCA TCTTTCAAAA TGTGGTGCGG TCCCCCGAGG TTCGTCCTCT TTTCTACCTG 
AGCCTCTCTG ATCTACTTCT GGGCATGTGC TGGCTTGCTG GAGCGCTTTT CTATAGCACA 
CCGACCTCCA AGCAGGATCT CATCTGCTAT AACCTGCAAG CAACAGGACA AATATTCTAT 
GTGGCCTCTT TCCTATACAC TGCCAACTAC ACCTGGCATC TGTACATGGA CCTCAAGGTG 
AAATACAACC AGAACCTTTT CAGGATGCCC CCCCAGGTTG TAGATTATGC AAGTTGCATT 
GGCCGAATAG CAACGATCTT GTCCAGCCTG ATCCCTTTCC TTCTCATGGT GCCTGTGTTT 
TGCCTGGGCA ACAGCAGTAA TTGCTACCAG AACTTCAGCC AGAAGCACGG ATGTCTCTTG 
ATGCACACGG AAATCGCCAT GCCCACCAAC GAGCCGCAAA CCCTGAGTGG CTCTGTTTGC 
CGTGCGATGC ATTTCTATGG CATTGGGATC TTCCTCATTT CCTTTCTGAT CAGCTTCATA 
GCCATTTTGG TTATACTCAG TCAAGCCCGA GGCTTGTACA AAAGGTTTGT AAACTCCACA 
GGATTCCTGG GGGATCAGCA GTGGGCCATG ATTAAGATGG TAGAACAACG AGTGGTTTTT 
TACCCCATCG CATTCTTCTG CTGCTGGGCC CCAGCTGTCC TCCTTGGAAT ACTGAAGCTG 
ACTGTTTCAA CCAACAGCAA AATCTACATG GCTCTTCTAA TTCTACAGGC TCTGACAGCA 
GCTTCCCAGG GGCTTCTGAA CTGCATCGTC TACGGCTGGA CGCAGCACGT CTTCCTCTCC 
CTCAAACGCA ACGCCTGCCG AGATGTCGAC ACTCAGACTC CTCTTCTACG CTCCCAGAAG 
AAGTTCTACT CCAGCACGCT CCCTGCCAGC CCACCAGATG CAGAGGGCTC CACGTCCACC 
CTGCTCTGA