DGCR8 cDNA ORF clone, Calidris pugnax(ruff)

Gene
Clones
gRNAs

Summary

Gene Symbol	DGCR8
Entrez Gene ID	106899801
Full Name	DGCR8, microprocessor complex subunit
Gene Type	protein-coding
Organism	Calidris pugnax(ruff)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014961725.1	XP_014817211.1	microprocessor complex subunit DGCR8

Pathways from BioSystems
Gene Ontology

The following DGCR8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DGCR8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCa59789	XM_014961725.1 Latest version!	Calidris pugnax DGCR8 microprocessor complex subunit (DGCR8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DGCR8 ( XM_014961725.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014817211.1 Calidris pugnax DGCR8 microprocessor complex subunit (DGCR8), mRNA.

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CloneID	OCa59789
Clone ID Related Accession (Same CDS sequence)	XM_014961725.1
Accession Version	XM_014961725.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	microprocessor complex subunit DGCR8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090783.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAAT ATGAAAATGT ACCACCCCTC CCTAAAGAAC CTGCAAGAGA AATGAATGTG 
GAGAATCATA CTTGTCCCCC ACCTCTGCCG CCTAACGAAC AGCCTCCACC ACCTCCCCTG 
CAAACGTCCA GTGACGCAGA GGTAATGGAC GTTGGCTCTG GTGGTGATGG ACAGTCAGAT 
ACCCCTGCTG GGGACACGCA CAGTATCTGC AGAACACAGC TTCTCACAAA GGGATCTGCC 
TGCTACAAAA GTCGTCTTAT AATAGATCCT AACAATAGTG ACCAAAGCCC AAGAACTGCT 
CGTCATGCAC CTTCAGTTCG CAAGTTCACC CCAGATCTTA AGTTACTTAA AGATGTAAAG 
ATAAGTGTTA GCTTTACAGA AAGCTGCAAA AGTAAAGATA GGAAAGTTCT GTACACTGGA 
GTGGAGCAGG ATTATAAGGC AGATACAGAT TTTGGTATTA ATAATGTAAA CGGGGATTTG 
CATGTTTGTC CTTTTGGTGG TAGTAATGGT AAAGCTGTAG GAGTAGGGGG TGAAAATGAT 
GACAAGAAGG ATGATGAAAA TGATATTGAT CAGGAAAAGA GAGTGGAATA TGCTGTTCTG 
GATGAGCTAG AAGACTTTAC CGACAATTTG ATGGAAATAG ATGAAGAAGG AGGAGGGTTC 
ACATCTAAAG CAATCGTTCA AAGAGATAAA GTGGATGAAG AAACCTTGAA TTACTCATAT 
GAGGATGACT TTGATAATGA TGTGGATGCT CTGCTGGAAG AGGGTCTTCG AGCACCTAAA 
ACGAGGAGAC TAGAAAATGA GAAATATGGT GGTGAAAGTG ATCACCAGTC TGATGGAGAA 
ACAAGTGTGC AGCCAATGAT CACCAAAATT AAAACTGTAC TTAAAAGTCG TGGCCGCCCT 
CCTACAGAAC CTTTGCCAGA TGGATGGATC ATGACATTCC ATAACTCAGG CATTCCTGTT 
TATCTGCACA GAGAATCTAG AGTTGTTACC TGGTCTAGAC CTTACTTCTT GGGAACAGGA 
AGCATAAGGA AACATGATCC TCCCCTCAGT AGCATTCCCT GCTTGCATTA CAAAAAAATG 
AAGGAAAATG AGGAAAGGGA ACAAAACAAT GACATAACCC CAAATGGGGA AGTGTCACCT 
GTAAAGCATC TAGATAAATC TTCCGAGCTG GATTGCCAAA CAGAGGAACC GGACTCTACT 
GCTGCTGATT CTGGGCCTTT AGATGACAAA GACCCCTCAG GGGGAGATGC AGCACAAGGA 
GCCTTAGGAC AAGTTAAGGC CAAAGTTGAA GTATGTAAAG ATGAATCTGT AGATCTGGAA 
GATTTTAGAT GCTATCTTGA AAAGCGTTTT GATTTTGAAC AAGTTACCGT AAAGAAATTC 
AGAACCTGGG CTGAGAGACG GCAGTTCAAT CGTGAGATGA AGCGGAAGCA GGCAGAGTCG 
GAGCGGCCAA TTCTGCCTGC CAATCAGAAA CTCATTACCT TGTCAGTGCA AGATGCACCT 
ACAAAGAAAG AATTTGTCAT TAATCCCAAT GGGAAATCTG AAGTTTGCAT ACTGCATGAA 
TATATGCAAC GAGTCCTAAA GGTTCGCCCT GTTTACAATT TCTTTGAATG TGAGAACCCA 
AGTGAACCTT TTGGAGCCTC GGTGATAATT GATGGAGTAA CATATGGGGC AGGAACTGCC 
AGCAGCAAAA AACTTGCCAA GAATAAAGCT GCTCGTGCTA CACTGGAAAT TCTTATTCCC 
GACTTTGTTA AACAGACCTC TGAGGAGAAG CCCAAAGACA GTGAAGAACT TGAGTATTTC 
AACCATATCA GTATTGAGGA CTCACGGGTA TATGAACTCA CCAGTAAAGC TGGACTCTTG 
TCTCCATATC AGATTCTCCA CGAGTGCCTT AAAAGAAACC ATGGAATGGG TGATACATCC 
ATAAAGTTTG AAGTGATTCC TGGTAAAAAT CAGAAGAGCG AATACGTCAT GACTTGTGGG 
AAGCACACGG TGCGAGGCTG GTGCAAAAAT AAGAGAGTGG GGAAACAATT AGCTTCTCAG 
AAAATCCTGC AGCTACTGCA TCCACATGTG AAAAACTGGG GCTCTCTTTT GCGTATGTAT 
GGTAGAGAGA GTAGCAAGAT GGTTAAACAG GAAACCTCCG ATAAAAGTGT GATAGAGCTT 
CAGCAGTATG CCAAAAAGAA CAAGCCAAAT CTGCACATCC TGAACAAGTT ACAGGAAGAA 
ATGAAAAAAC TGGCACAAGA AAGAGAGGAA ACCCGAAAGA AGCCCAAGAT GACCATAGTG 
GAGTCGGCTC AGCCCGGCAG CGAGCCTCTG TGCACCGTCG ACGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014817211.1
CDS	397..2724
Translation	MEKYENVPPLPKEPAREMNVENHTCPPPLPPNEQPPPPPLQTSSDAEVMDVGSGGDGQSDTPAGDTHSICRTQLLTKGSACYKSRLIIDPNNSDQSPRTARHAPSVRKFTPDLKLLKDVKISVSFTESCKSKDRKVLYTGVEQDYKADTDFGINNVNGDLHVCPFGGSNGKAVGVGGENDDKKDDENDIDQEKRVEYAVLDELEDFTDNLMEIDEEGGGFTSKAIVQRDKVDEETLNYSYEDDFDNDVDALLEEGLRAPKTRRLENEKYGGESDHQSDGETSVQPMITKIKTVLKSRGRPPTEPLPDGWIMTFHNSGIPVYLHRESRVVTWSRPYFLGTGSIRKHDPPLSSIPCLHYKKMKENEEREQNNDITPNGEVSPVKHLDKSSELDCQTEEPDSTAADSGPLDDKDPSGGDAAQGALGQVKAKVEVCKDESVDLEDFRCYLEKRFDFEQVTVKKFRTWAERRQFNREMKRKQAESERPILPANQKLITLSVQDAPTKKEFVINPNGKSEVCILHEYMQRVLKVRPVYNFFECENPSEPFGASVIIDGVTYGAGTASSKKLAKNKAARATLEILIPDFVKQTSEEKPKDSEELEYFNHISIEDSRVYELTSKAGLLSPYQILHECLKRNHGMGDTSIKFEVIPGKNQKSEYVMTCGKHTVRGWCKNKRVGKQLASQKILQLLHPHVKNWGSLLRMYGRESSKMVKQETSDKSVIELQQYAKKNKPNLHILNKLQEEMKKLAQEREETRKKPKMTIVESAQPGSEPLCTVDV

Target ORF information:

RefSeq Version	XM_014961725.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax DGCR8 microprocessor complex subunit (DGCR8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014961725.1

ORF Insert Sequence:

ATGGAGAAAT ATGAAAATGT ACCACCCCTC CCTAAAGAAC CTGCAAGAGA AATGAATGTG 
GAGAATCATA CTTGTCCCCC ACCTCTGCCG CCTAACGAAC AGCCTCCACC ACCTCCCCTG 
CAAACGTCCA GTGACGCAGA GGTAATGGAC GTTGGCTCTG GTGGTGATGG ACAGTCAGAT 
ACCCCTGCTG GGGACACGCA CAGTATCTGC AGAACACAGC TTCTCACAAA GGGATCTGCC 
TGCTACAAAA GTCGTCTTAT AATAGATCCT AACAATAGTG ACCAAAGCCC AAGAACTGCT 
CGTCATGCAC CTTCAGTTCG CAAGTTCACC CCAGATCTTA AGTTACTTAA AGATGTAAAG 
ATAAGTGTTA GCTTTACAGA AAGCTGCAAA AGTAAAGATA GGAAAGTTCT GTACACTGGA 
GTGGAGCAGG ATTATAAGGC AGATACAGAT TTTGGTATTA ATAATGTAAA CGGGGATTTG 
CATGTTTGTC CTTTTGGTGG TAGTAATGGT AAAGCTGTAG GAGTAGGGGG TGAAAATGAT 
GACAAGAAGG ATGATGAAAA TGATATTGAT CAGGAAAAGA GAGTGGAATA TGCTGTTCTG 
GATGAGCTAG AAGACTTTAC CGACAATTTG ATGGAAATAG ATGAAGAAGG AGGAGGGTTC 
ACATCTAAAG CAATCGTTCA AAGAGATAAA GTGGATGAAG AAACCTTGAA TTACTCATAT 
GAGGATGACT TTGATAATGA TGTGGATGCT CTGCTGGAAG AGGGTCTTCG AGCACCTAAA 
ACGAGGAGAC TAGAAAATGA GAAATATGGT GGTGAAAGTG ATCACCAGTC TGATGGAGAA 
ACAAGTGTGC AGCCAATGAT CACCAAAATT AAAACTGTAC TTAAAAGTCG TGGCCGCCCT 
CCTACAGAAC CTTTGCCAGA TGGATGGATC ATGACATTCC ATAACTCAGG CATTCCTGTT 
TATCTGCACA GAGAATCTAG AGTTGTTACC TGGTCTAGAC CTTACTTCTT GGGAACAGGA 
AGCATAAGGA AACATGATCC TCCCCTCAGT AGCATTCCCT GCTTGCATTA CAAAAAAATG 
AAGGAAAATG AGGAAAGGGA ACAAAACAAT GACATAACCC CAAATGGGGA AGTGTCACCT 
GTAAAGCATC TAGATAAATC TTCCGAGCTG GATTGCCAAA CAGAGGAACC GGACTCTACT 
GCTGCTGATT CTGGGCCTTT AGATGACAAA GACCCCTCAG GGGGAGATGC AGCACAAGGA 
GCCTTAGGAC AAGTTAAGGC CAAAGTTGAA GTATGTAAAG ATGAATCTGT AGATCTGGAA 
GATTTTAGAT GCTATCTTGA AAAGCGTTTT GATTTTGAAC AAGTTACCGT AAAGAAATTC 
AGAACCTGGG CTGAGAGACG GCAGTTCAAT CGTGAGATGA AGCGGAAGCA GGCAGAGTCG 
GAGCGGCCAA TTCTGCCTGC CAATCAGAAA CTCATTACCT TGTCAGTGCA AGATGCACCT 
ACAAAGAAAG AATTTGTCAT TAATCCCAAT GGGAAATCTG AAGTTTGCAT ACTGCATGAA 
TATATGCAAC GAGTCCTAAA GGTTCGCCCT GTTTACAATT TCTTTGAATG TGAGAACCCA 
AGTGAACCTT TTGGAGCCTC GGTGATAATT GATGGAGTAA CATATGGGGC AGGAACTGCC 
AGCAGCAAAA AACTTGCCAA GAATAAAGCT GCTCGTGCTA CACTGGAAAT TCTTATTCCC 
GACTTTGTTA AACAGACCTC TGAGGAGAAG CCCAAAGACA GTGAAGAACT TGAGTATTTC 
AACCATATCA GTATTGAGGA CTCACGGGTA TATGAACTCA CCAGTAAAGC TGGACTCTTG 
TCTCCATATC AGATTCTCCA CGAGTGCCTT AAAAGAAACC ATGGAATGGG TGATACATCC 
ATAAAGTTTG AAGTGATTCC TGGTAAAAAT CAGAAGAGCG AATACGTCAT GACTTGTGGG 
AAGCACACGG TGCGAGGCTG GTGCAAAAAT AAGAGAGTGG GGAAACAATT AGCTTCTCAG 
AAAATCCTGC AGCTACTGCA TCCACATGTG AAAAACTGGG GCTCTCTTTT GCGTATGTAT 
GGTAGAGAGA GTAGCAAGAT GGTTAAACAG GAAACCTCCG ATAAAAGTGT GATAGAGCTT 
CAGCAGTATG CCAAAAAGAA CAAGCCAAAT CTGCACATCC TGAACAAGTT ACAGGAAGAA 
ATGAAAAAAC TGGCACAAGA AAGAGAGGAA ACCCGAAAGA AGCCCAAGAT GACCATAGTG 
GAGTCGGCTC AGCCCGGCAG CGAGCCTCTG TGCACCGTCG ACGTGTAA