TMEM132C cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	TMEM132C
Entrez Gene ID	403528
Full Name	transmembrane protein 132C
General protein information	Preferred Names transmembrane protein 132C Names transmembrane protein 132C
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001197119.1	NP_001184048.1	transmembrane protein 132C
XM_014107711.1	XP_013963186.1	transmembrane protein 132C isoform X1
XM_022410148.1	XP_022265856.1	transmembrane protein 132C isoform X1

Pathways from BioSystems

Homologies

	TMEM132C	XP_001106655.2
Rattus norvegicus (Norway rat)	Tmem132c	XP_002728014.2
Homo sapiens (human)	TMEM132C	NP_001129575.2
Bos taurus (cattle)	TMEM132C	XP_002694485.1
Pan troglodytes (chimpanzee)	TMEM132C	XP_522557.3
Gallus gallus (chicken)	TMEM132C	XP_004945578.1
Danio rerio (zebrafish)	LOC556622	XP_684556.5
Xenopus tropicalis (tropical clawed frog)	tmem132c	XP_002935335.2
Canis lupus familiaris (dog)	TMEM132C	NP_001184048.1
Mus musculus (house mouse)	Tmem132c	NP_780641.2

Gene Ontology
Bibliography

Related articles in PubMed

A method for the large-scale cloning of nuclear proteins and nuclear targeting sequences on a functional basis.
Pichon B, Mercan D, Pouillon V, Christophe-Hobertus C, Christophe D
Analytical biochemistry284(2)231-9(2000 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following TMEM132C gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TMEM132C cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe33750	NM_001197119.1 Latest version!	Canis lupus familiaris transmembrane protein 132C (TMEM132C), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OCe297367	XM_022410148.1 Latest version!	Canis lupus familiaris transmembrane protein 132C (TMEM132C), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OCe43183	XM_014107711.1 Latest version!	Canis lupus familiaris transmembrane protein 132C (TMEM132C), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TMEM132C ( NM_001197119.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001184048.1 Canis lupus familiaris transmembrane protein 132C (TMEM132C), mRNA.

Price & Availability↑

CloneID	OCe33750
Clone ID Related Accession (Same CDS sequence)	NM_001197119.1
Accession Version	NM_001197119.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-22
Organism	Canis lupus familiaris(dog)
Product	transmembrane protein 132C
Comment	Comment: INFERRED REFSEQ: This record is predicted by genome sequence analysis and is not yet supported by experimental evidence. The reference sequence was derived from AAEX03014613.1, AAEX03014612.1, AAEX03014611.1 and AAEX03014610.1. On Oct 8, 2010 this sequence version replaced XM_543360.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA103936001, SAMEA103936002 [ECO:0000350] ##Evidence-Data-END##

ATGGCCACCA TGCTATTCGC CAAGCTCAAC TTCAGGAACT TGATCGAGGG CCGGGGGATC 
ACAGATAATG TGCCACGGTT CTCTTCTCTG CCACCCTTCC TGCCTGTGAG CTACCACATC 
CTTGGAGCAG AGACCTCCTT CTTCCTGAAG GAGGCCAACC AGGACGTCCT GCGCAACTCC 
AGCCTGCACA CCAGGGTGGA GTCCTTCTTC ACCTACAAGG CCAACCGGCC CCCAGTGCTC 
AACGCCAGCT ACGGGCCCTT CTCCATCGAG CAGGTGGTGC CTCAGGACCT CCTGCTGCCC 
TCCAGCCCCT TCGGAGCCAC CAGCAAGCTC TCCCTCAACT GGAGGCTGAG GGCGCACATC 
CTGCGGGACA AGGTGTACCT GAGCCGGCCC CGGGTGCAGG TGCTCTTCCA CCTGCTGGGC 
CGGGACTGGG CGGCACAGAG CCCTGGCGAG CGGCTGCCCT GCCTGCGGCT GTTCGCCTTC 
CGGGAGACCC GGGAGGTGCG GGCCGGCTGC CGGCTGCAGG GCGCCCTGGG GCTGTGCGTG 
GCCGAACTGG AGCTGCTGGC CGCCTGGTTC GGGCCCCCCA CCGTGGTGGC CGGGAGGAAG 
CGGGCGCCTG GGCCGCCCGA GGGGAGCCCC GTGGAGCTCT ACTACTCCGT GCAGCCGGGG 
GATGCGCGCG GGGACTGTGC GGGCGGCGGC GGCGACGTCA GGAAGGGCAA CGCCATCCGG 
CCTGGGAAGG ACGGGCTGGA TGAGGCCGTG CCCCACCTGC AGAGGATCGG CGCCGTCAGC 
CTCTACCGGG CCCAGGACAG CACCCAGCTC AGCGAGCTGC GTTTGGACAG CAACGTGGTC 
ATCTGGCTGC CCTCCCGGCC CGTCAAGCAA GGAGATGTGG TCACCGCCTA CGTCACCATC 
GCCAGCAACT CCACTGTGGA CCTTTTCATC CTGAGAGCCA AGGTGAAGAA GGGGGTGAAC 
ATCCTGGGCA CTCAGACCAG CGAGCCCCGG CAGTGGGATG TCAAACAGGA GATGGGGAAT 
GGCGGAAAGC ATGCTACCAC CACCGTGCTG TGCCAGCGCC TGGGGTCCAG CACACGTAAC 
AGAAGCAGCA GCCTGTTCAG TGAGGTTATG CAGATGAACT TTGAAATAGC CAGCTTCAGT 
AGCCTCTCGG GGACTCAGCC CATCGCCTGG CAGGTGGAAT ATCCACGGAG AGCGACCACC 
GACACCGCTG TGTCCGAGAT CTTCATCAGC CAGAAGGACC TGGCTGGCAT CGTTCCTCTA 
GCCACGGACA CGGAAATTCT GAACACTGCC ATCCTCACGG GAAAGACAGT GGTCCTGCCC 
ATCAAGGTGG TCTCTGTGGA GGAGAACAGT GCCGTGATGG ATATCTCCGA GTCGGTGGAG 
TGCAAGTCCA CAGACGAGGA TGTCATCAAG GTGTCTGAAA GATGTGACTA CGTCTTTGTC 
AATGGCAAAG AGATGAAGGG CAAGGTGGAT GCGGTGGTGA ACTTCACCTA CCAGCACCTG 
AGTGCCTCCC TGCACATCAC CGTGTGGGTG CCCCGGCTAC CCCTACAGAT TGAGGTCTCT 
GACACAGAGC TGAGCCAGAT CAAAGGCTGG AGGGTCCCCA TTGTGTCCAG TAAGAGGCCC 
ACTCGAGAGA GCGAGGATGA AGACGAGGAG GAGCGGCGGG GCCGAGGCTG TGCCCTGCAG 
TACCAGCACG CCACGGTGCG GGTCCTCACC CAGTTCGTGT CGGAGGGCGC TGGCCCGTGG 
GGCCAGCCGA GCCACCTTCT CAGTCCCGAC TGGCAAGTCG ACATCACCCA CCTGGTTGCA 
GACTTCATGA AGCTGGAGGA ACCTCACGTG GCCACACTCC AGGACAGCAG GATCCTGGTC 
GGGCGGGAGG TTGGGATGAC CACCATCCAG GTGTTGTCTC CACTCTCTGA CTCCATCCTG 
GCAGAGAAGA CGGTGACCGT GTTAGATGAC AAGGTATCGG TGACAGACTT GGCCATCCAG 
CTCGTGGCTG GGCTGTCTGT CACCCTCCAC CCCAGCACGG AGAACAGCAA GGCCATCACT 
GCTGTGGCCA CAGCTGAGGA GCTGCTGCGG ACCCCCAAAC AGGAGGCCGT GGTCAGCACT 
TGGCTCCAGC TCAGCGACGG CTCCGCCACC CCCCTGGACA TCTACGACAC CAAGGACTTC 
ACCCTGACCG CCACCTCCCT GAACGAGGCC GTCGTGTCCA CCCCCCAGGC CCGCTCTCCC 
AGATGGCCGG TGGTGATGGC CGAAGGGGAA GGCCAGGGGC CGCTGGTGCG AGTGGACATG 
TCCATCGCCG AGGCCTGCCA GAAGTCCAAG CGCAAGAGCG TCCTGGCCGT GGGTGTGGGC 
AGCGTCAGGG TCAAGTTTGG CCAGGGCAAC GCCGACTCCA GCCGCGGCGC GGACGGCGAC 
AGTGGCGAGA TCAAGAACCA CGCCAGCGAC CGCCGGCAGA AGAGCCAGGA GCCCGAGGGG 
CACCTCCAAG GCAGCCCGGG GGAGCGCGAG GACGGCGCCC TGCAGAGAGG CGACAGCACG 
GCCAGGCCGC TCCTGGACAA CAGGGTGGTG AAGAGCGGCC GGCCGGACGC GGGCAGGCCG 
TCCGGGGGGG ACCAGCTGCA GAACATCCCC CTGGACTTCG CCAACTTCCC GGCGCAGGTG 
GAGCTGCCCC GGGCGGGGGG CGGCCTGGGG GCCAGCGACC TGGTGCAGAC GCCCCGGGGC 
CTGAGCGACC TGGAGATCGG CATGTACGCC CTGCTCGGCG TCTTCTGCTT GGCCATCCTT 
GTCTTCCTCA TCAACTGCGC CACCTTCGCC CTCAGGTACC GCCACAAGCA GGTGCCCCTG 
GAAGGCCAGG CCTCTGTGAC CCACTCGCAC GACTGGGTGT GGCTGGGCAA CGAGGCGGAG 
CTCCTGGAGC ACGCGGGCGA GGGGTCGCCA CCGCAGGACG AGCACACGAC TGTCCTGGAC 
CGCGGGCCGG GCGGCAGCGA CGACGGCAGC CGGCTGCTGC TCAACGGCGG CGCCCGGCAG 
CACGTGCAGG GCCAGGTGCA CCGGGCGGGC TCGGCGGGCA GGCCGGCCAG GGACCCGAAG 
CTCGAGCCCC TGCATTCGCC CACCTCCAAA AGGAAGAAAG TGAAGTTCAC CACCTTCACC 
ACCATCCCGC CCGACGACGG CTGCCCCACC GTGAACTCCA TCCTGGGGGG CGGCGGCGGC 
GAGGACATCA AGTGGGTGTG CCAGGACGTG TCCCCGGGCG CCCCCAAGGA GCTCAGAAAC 
TACCTGGAGA AATTCAAGGA CCCGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001184048.1
CDS	1..3270
Translation	MATMLFAKLNFRNLIEGRGITDNVPRFSSLPPFLPVSYHILGAETSFFLKEANQDVLRNSSLHTRVESFFTYKANRPPVLNASYGPFSIEQVVPQDLLLPSSPFGATSKLSLNWRLRAHILRDKVYLSRPRVQVLFHLLGRDWAAQSPGERLPCLRLFAFRETREVRAGCRLQGALGLCVAELELLAAWFGPPTVVAGRKRAPGPPEGSPVELYYSVQPGDARGDCAGGGGDVRKGNAIRPGKDGLDEAVPHLQRIGAVSLYRAQDSTQLSELRLDSNVVIWLPSRPVKQGDVVTAYVTIASNSTVDLFILRAKVKKGVNILGTQTSEPRQWDVKQEMGNGGKHATTTVLCQRLGSSTRNRSSSLFSEVMQMNFEIASFSSLSGTQPIAWQVEYPRRATTDTAVSEIFISQKDLAGIVPLATDTEILNTAILTGKTVVLPIKVVSVEENSAVMDISESVECKSTDEDVIKVSERCDYVFVNGKEMKGKVDAVVNFTYQHLSASLHITVWVPRLPLQIEVSDTELSQIKGWRVPIVSSKRPTRESEDEDEEERRGRGCALQYQHATVRVLTQFVSEGAGPWGQPSHLLSPDWQVDITHLVADFMKLEEPHVATLQDSRILVGREVGMTTIQVLSPLSDSILAEKTVTVLDDKVSVTDLAIQLVAGLSVTLHPSTENSKAITAVATAEELLRTPKQEAVVSTWLQLSDGSATPLDIYDTKDFTLTATSLNEAVVSTPQARSPRWPVVMAEGEGQGPLVRVDMSIAEACQKSKRKSVLAVGVGSVRVKFGQGNADSSRGADGDSGEIKNHASDRRQKSQEPEGHLQGSPGEREDGALQRGDSTARPLLDNRVVKSGRPDAGRPSGGDQLQNIPLDFANFPAQVELPRAGGGLGASDLVQTPRGLSDLEIGMYALLGVFCLAILVFLINCATFALRYRHKQVPLEGQASVTHSHDWVWLGNEAELLEHAGEGSPPQDEHTTVLDRGPGGSDDGSRLLLNGGARQHVQGQVHRAGSAGRPARDPKLEPLHSPTSKRKKVKFTTFTTIPPDDGCPTVNSILGGGGGEDIKWVCQDVSPGAPKELRNYLEKFKDPA

Target ORF information:

RefSeq Version	NM_001197119.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris transmembrane protein 132C (TMEM132C), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001197119.1

ORF Insert Sequence:

ATGGCCACCA TGCTATTCGC CAAGCTCAAC TTCAGGAACT TGATCGAGGG CCGGGGGATC 
ACAGATAATG TGCCACGGTT CTCTTCTCTG CCACCCTTCC TGCCTGTGAG CTACCACATC 
CTTGGAGCAG AGACCTCCTT CTTCCTGAAG GAGGCCAACC AGGACGTCCT GCGCAACTCC 
AGCCTGCACA CCAGGGTGGA GTCCTTCTTC ACCTACAAGG CCAACCGGCC CCCAGTGCTC 
AACGCCAGCT ACGGGCCCTT CTCCATCGAG CAGGTGGTGC CTCAGGACCT CCTGCTGCCC 
TCCAGCCCCT TCGGAGCCAC CAGCAAGCTC TCCCTCAACT GGAGGCTGAG GGCGCACATC 
CTGCGGGACA AGGTGTACCT GAGCCGGCCC CGGGTGCAGG TGCTCTTCCA CCTGCTGGGC 
CGGGACTGGG CGGCACAGAG CCCTGGCGAG CGGCTGCCCT GCCTGCGGCT GTTCGCCTTC 
CGGGAGACCC GGGAGGTGCG GGCCGGCTGC CGGCTGCAGG GCGCCCTGGG GCTGTGCGTG 
GCCGAACTGG AGCTGCTGGC CGCCTGGTTC GGGCCCCCCA CCGTGGTGGC CGGGAGGAAG 
CGGGCGCCTG GGCCGCCCGA GGGGAGCCCC GTGGAGCTCT ACTACTCCGT GCAGCCGGGG 
GATGCGCGCG GGGACTGTGC GGGCGGCGGC GGCGACGTCA GGAAGGGCAA CGCCATCCGG 
CCTGGGAAGG ACGGGCTGGA TGAGGCCGTG CCCCACCTGC AGAGGATCGG CGCCGTCAGC 
CTCTACCGGG CCCAGGACAG CACCCAGCTC AGCGAGCTGC GTTTGGACAG CAACGTGGTC 
ATCTGGCTGC CCTCCCGGCC CGTCAAGCAA GGAGATGTGG TCACCGCCTA CGTCACCATC 
GCCAGCAACT CCACTGTGGA CCTTTTCATC CTGAGAGCCA AGGTGAAGAA GGGGGTGAAC 
ATCCTGGGCA CTCAGACCAG CGAGCCCCGG CAGTGGGATG TCAAACAGGA GATGGGGAAT 
GGCGGAAAGC ATGCTACCAC CACCGTGCTG TGCCAGCGCC TGGGGTCCAG CACACGTAAC 
AGAAGCAGCA GCCTGTTCAG TGAGGTTATG CAGATGAACT TTGAAATAGC CAGCTTCAGT 
AGCCTCTCGG GGACTCAGCC CATCGCCTGG CAGGTGGAAT ATCCACGGAG AGCGACCACC 
GACACCGCTG TGTCCGAGAT CTTCATCAGC CAGAAGGACC TGGCTGGCAT CGTTCCTCTA 
GCCACGGACA CGGAAATTCT GAACACTGCC ATCCTCACGG GAAAGACAGT GGTCCTGCCC 
ATCAAGGTGG TCTCTGTGGA GGAGAACAGT GCCGTGATGG ATATCTCCGA GTCGGTGGAG 
TGCAAGTCCA CAGACGAGGA TGTCATCAAG GTGTCTGAAA GATGTGACTA CGTCTTTGTC 
AATGGCAAAG AGATGAAGGG CAAGGTGGAT GCGGTGGTGA ACTTCACCTA CCAGCACCTG 
AGTGCCTCCC TGCACATCAC CGTGTGGGTG CCCCGGCTAC CCCTACAGAT TGAGGTCTCT 
GACACAGAGC TGAGCCAGAT CAAAGGCTGG AGGGTCCCCA TTGTGTCCAG TAAGAGGCCC 
ACTCGAGAGA GCGAGGATGA AGACGAGGAG GAGCGGCGGG GCCGAGGCTG TGCCCTGCAG 
TACCAGCACG CCACGGTGCG GGTCCTCACC CAGTTCGTGT CGGAGGGCGC TGGCCCGTGG 
GGCCAGCCGA GCCACCTTCT CAGTCCCGAC TGGCAAGTCG ACATCACCCA CCTGGTTGCA 
GACTTCATGA AGCTGGAGGA ACCTCACGTG GCCACACTCC AGGACAGCAG GATCCTGGTC 
GGGCGGGAGG TTGGGATGAC CACCATCCAG GTGTTGTCTC CACTCTCTGA CTCCATCCTG 
GCAGAGAAGA CGGTGACCGT GTTAGATGAC AAGGTATCGG TGACAGACTT GGCCATCCAG 
CTCGTGGCTG GGCTGTCTGT CACCCTCCAC CCCAGCACGG AGAACAGCAA GGCCATCACT 
GCTGTGGCCA CAGCTGAGGA GCTGCTGCGG ACCCCCAAAC AGGAGGCCGT GGTCAGCACT 
TGGCTCCAGC TCAGCGACGG CTCCGCCACC CCCCTGGACA TCTACGACAC CAAGGACTTC 
ACCCTGACCG CCACCTCCCT GAACGAGGCC GTCGTGTCCA CCCCCCAGGC CCGCTCTCCC 
AGATGGCCGG TGGTGATGGC CGAAGGGGAA GGCCAGGGGC CGCTGGTGCG AGTGGACATG 
TCCATCGCCG AGGCCTGCCA GAAGTCCAAG CGCAAGAGCG TCCTGGCCGT GGGTGTGGGC 
AGCGTCAGGG TCAAGTTTGG CCAGGGCAAC GCCGACTCCA GCCGCGGCGC GGACGGCGAC 
AGTGGCGAGA TCAAGAACCA CGCCAGCGAC CGCCGGCAGA AGAGCCAGGA GCCCGAGGGG 
CACCTCCAAG GCAGCCCGGG GGAGCGCGAG GACGGCGCCC TGCAGAGAGG CGACAGCACG 
GCCAGGCCGC TCCTGGACAA CAGGGTGGTG AAGAGCGGCC GGCCGGACGC GGGCAGGCCG 
TCCGGGGGGG ACCAGCTGCA GAACATCCCC CTGGACTTCG CCAACTTCCC GGCGCAGGTG 
GAGCTGCCCC GGGCGGGGGG CGGCCTGGGG GCCAGCGACC TGGTGCAGAC GCCCCGGGGC 
CTGAGCGACC TGGAGATCGG CATGTACGCC CTGCTCGGCG TCTTCTGCTT GGCCATCCTT 
GTCTTCCTCA TCAACTGCGC CACCTTCGCC CTCAGGTACC GCCACAAGCA GGTGCCCCTG 
GAAGGCCAGG CCTCTGTGAC CCACTCGCAC GACTGGGTGT GGCTGGGCAA CGAGGCGGAG 
CTCCTGGAGC ACGCGGGCGA GGGGTCGCCA CCGCAGGACG AGCACACGAC TGTCCTGGAC 
CGCGGGCCGG GCGGCAGCGA CGACGGCAGC CGGCTGCTGC TCAACGGCGG CGCCCGGCAG 
CACGTGCAGG GCCAGGTGCA CCGGGCGGGC TCGGCGGGCA GGCCGGCCAG GGACCCGAAG 
CTCGAGCCCC TGCATTCGCC CACCTCCAAA AGGAAGAAAG TGAAGTTCAC CACCTTCACC 
ACCATCCCGC CCGACGACGG CTGCCCCACC GTGAACTCCA TCCTGGGGGG CGGCGGCGGC 
GAGGACATCA AGTGGGTGTG CCAGGACGTG TCCCCGGGCG CCCCCAAGGA GCTCAGAAAC 
TACCTGGAGA AATTCAAGGA CCCGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM132C ( XM_022410148.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022265856.1 Canis lupus familiaris transmembrane protein 132C (TMEM132C), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe297367
Clone ID Related Accession (Same CDS sequence)	XM_022410148.1
Accession Version	XM_022410148.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	transmembrane protein 132C isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006608.3) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGACCGTGA TCGAGGGCCG GGGGATCACA GATAATGTGC CACGGTTCTC TTCTCTGCCA 
CCCTTCCTGC CTGTGAGCTA CCACATCCTT GGAGCAGAGA CCTCCTTCTT CCTGAAGGAG 
GCCAACCAGG ACGTCCTGCG CAACTCCAGC CTGCACACCA GGGTGGAGTC CTTCTTCACC 
TACAAGGCCA ACCGGCCCCC AGTGCTCAAC GCCAGCTACG GGCCCTTCTC CATCGAGCAG 
GTGGTGCCTC AGGACCTCCT GCTGCCCTCC AGCCCCTTCG GAGCCACCAG CAAGCTCTCC 
CTCAACTGGA GGCTGAGGGC GCACATCCTG CGGGACAAGG TGTACCTGAG CCGGCCCCGG 
GTGCAGGTGC TCTTCCACCT GCTGGGCCGG GACTGGGCGG CACAGAGCCC TGGCGAGCGG 
CTGCCCTGCC TGCGGCTGTT CGCCTTCCGG GAGACCCGGG AGGTGCGGGC CGGCTGCCGG 
CTGCAGGGCG CCCTGGGGCT GTGCGTGGCC GAACTGGAGC TGCTGGCCGC CTGGTTCGGG 
CCCCCCACCG TGGTGGCCGG GAGGAAGCGG GCGCCTGGGC CGCCCGAGGG GAGCCCCGTG 
GAGCTCTACT ACTCCGTGCA GCCGGGGGAT GCGCGCGGGG ACTGTGCGGG CGGCGGCGGC 
GACGTCAGGA AGGGCAACGC CATCCGGCCT GGGAAGGACG GGCTGGATGA GGCCGTGCCC 
CACCTGCAGA GGATCGGCGC CGTCAGCCTC TACCGGGCCC AGGACAGCAC CCAGCTCAGC 
GAGCTGCGTT TGGACAGCAA CGTGGTCATC TGGCTGCCCT CCCGGCCCGT CAAGCAAGGA 
GATGTGGTCA CCGCCTACGT CACCATCGCC AGCAACTCCA CTGTGGACCT TTTCATCCTG 
AGAGCCAAGG TGAAGAAGGG GGTGAACATC CTGGGCACTC AGACCAGCGA GCCCCGGCAG 
TGGGATGTCA AACAGGAGAT GGGGAATGGC GGAAAGCATG CTACCACCAC CGTGCTGTGC 
CAGCGCCTGG GGTCCAGCAC ACGTAACAGA AGCAGCAGCC TGTTCAGTGA GGTTATGCAG 
ATGAACTTTG AAATAGCCAG CTTCAGTAGC CTCTCGGGGA CTCAGCCCAT CGCCTGGCAG 
GTGGAATATC CACGGAGAGC GACCACCGAC ACCGCTGTGT CCGAGATCTT CATCAGCCAG 
AAGGACCTGG CTGGCATCGT TCCTCTAGCC ACGGACACGG AAATTCTGAA CACTGCCATC 
CTCACGGGAA AGACAGTGGT CCTGCCCATC AAGGTGGTCT CTGTGGAGGA GAACAGTGCC 
GTGATGGATA TCTCCGAGTC GGTGGAGTGC AAGTCCACAG ACGAGGATGT CATCAAGGTG 
TCTGAAAGAT GTGACTACGT CTTTGTCAAT GGCAAAGAGA TGAAGGGCAA GGTGGATGCG 
GTGGTGAACT TCACCTACCA GCACCTGAGT GCCTCCCTGC ACATCACCGT GTGGGTGCCC 
CGGCTACCCC TACAGATTGA GGTCTCTGAC ACAGAGCTGA GCCAGATCAA AGGCTGGAGG 
GTCCCCATTG TGTCCAGTAA GAGGCCCACT CGAGAGAGCG AGGATGAAGA CGAGGAGGAG 
CGGCGGGGCC GAGGCTGTGC CCTGCAGTAC CAGCACGCCA CGGTGCGGGT CCTCACCCAG 
TTCGTGTCGG AGGGCGCTGG CCCGTGGGGC CAGCCGAGCC ACCTTCTCAG TCCCGACTGG 
CAAGTCGACA TCACCCACCT GGTTGCAGAC TTCATGAAGC TGGAGGAACC TCACGTGGCC 
ACACTCCAGG ACAGCAGGAT CCTGGTCGGG CGGGAGGTTG GGATGACCAC CATCCAGGTG 
TTGTCTCCAC TCTCTGACTC CATCCTGGCA GAGAAGACGG TGACCGTGTT AGATGACAAG 
GTATCGGTGA CAGACTTGGC CATCCAGCTC GTGGCTGGGC TGTCTGTCAC CCTCCACCCC 
AGCACGGAGA ACAGCAAGGC CATCACTGCT GTGGCCACAG CTGAGGAGCT GCTGCGGACC 
CCCAAACAGG AGGCCGTGGT CAGCACTTGG CTCCAGCTCA GCGACGGCTC CGCCACCCCC 
CTGGACATCT ACGACACCAA GGACTTCACC CTGACCGCCA CCTCCCTGAA CGAGGCCGTC 
GTGTCCACCC CCCAGGCCCG CTCTCCCAGA TGGCCGGTGG TGATGGCCGA AGGGGAAGGC 
CAGGGGCCGC TGGTGCGAGT GGACATGTCC ATCGCCGAGG CCTGCCAGAA GTCCAAGCGC 
AAGAGCGTCC TGGCCGTGGG TGTGGGCAGC GTCAGGGTCA AGTTTGGCCA GGGCAACGCC 
GACTCCAGCC GCGGCGCGGA CGGCGACAGT GGCGAGATCA AGAACCACGC CAGCGACCGC 
CGGCAGAAGA GCCAGGAGCC CGAGGGGCAC CTCCAAGGCA GCCCGGGGGA GCGCGAGGAC 
GGCGCCCTGC AGAGAGGCGA CAGCACGGCC AGGCCGCTCC TGGACAACAG GGTGGTGAAG 
AGCGGCCGGC CGGACGCGGG CAGGCCGTCC GGGGGGGACC AGCTGCAGAA CATCCCCCTG 
GACTTCGCCA ACTTCCCGGC GCAGGTGGAG CTGCCCCGGG CGGGGGGCGG CCTGGGGGCC 
AGCGACCTGG TGCAGACGCC CCGGGGCCTG AGCGACCTGG AGATCGGCAT GTACGCCCTG 
CTCGGCGTCT TCTGCTTGGC CATCCTTGTC TTCCTCATCA ACTGCGCCAC CTTCGCCCTC 
AGGTACCGCC ACAAGCAGGT GCCCCTGGAA GGCCAGGCCT CTGTGACCCA CTCGCACGAC 
TGGGTGTGGC TGGGCAACGA GGCGGAGCTC CTGGAGCACG CGGGCGAGGG GTCGCCACCG 
CAGGACGAGC ACACGACTGT CCTGGACCGC GGGCCGGGCG GCAGCGACGA CGGCAGCCGG 
CTGCTGCTCA ACGGCGGCGC CCGGCAGCAC GTGCAGGGCC AGGTGCACCG GGCGGGCTCG 
GCGGGCAGGC CGGCCAGGGA CCCGAAGCTC GAGCCCCTGC ATTCGCCCAC CTCCAAAAGG 
AAGAAAGTGA AGTTCACCAC CTTCACCACC ATCCCGCCCG ACGACGGCTG CCCCACCGTG 
AACTCCATCC TGGGGGGCGG CGGCGGCGAG GACATCAAGT GGGTGTGCCA GGACGTGTCC 
CCGGGCGCCC CCAAGGAGCT CAGAAACTAC CTGGAGAAAT TCAAGGACCC GGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022265856.1
CDS	1..3237
Translation	MTVIEGRGITDNVPRFSSLPPFLPVSYHILGAETSFFLKEANQDVLRNSSLHTRVESFFTYKANRPPVLNASYGPFSIEQVVPQDLLLPSSPFGATSKLSLNWRLRAHILRDKVYLSRPRVQVLFHLLGRDWAAQSPGERLPCLRLFAFRETREVRAGCRLQGALGLCVAELELLAAWFGPPTVVAGRKRAPGPPEGSPVELYYSVQPGDARGDCAGGGGDVRKGNAIRPGKDGLDEAVPHLQRIGAVSLYRAQDSTQLSELRLDSNVVIWLPSRPVKQGDVVTAYVTIASNSTVDLFILRAKVKKGVNILGTQTSEPRQWDVKQEMGNGGKHATTTVLCQRLGSSTRNRSSSLFSEVMQMNFEIASFSSLSGTQPIAWQVEYPRRATTDTAVSEIFISQKDLAGIVPLATDTEILNTAILTGKTVVLPIKVVSVEENSAVMDISESVECKSTDEDVIKVSERCDYVFVNGKEMKGKVDAVVNFTYQHLSASLHITVWVPRLPLQIEVSDTELSQIKGWRVPIVSSKRPTRESEDEDEEERRGRGCALQYQHATVRVLTQFVSEGAGPWGQPSHLLSPDWQVDITHLVADFMKLEEPHVATLQDSRILVGREVGMTTIQVLSPLSDSILAEKTVTVLDDKVSVTDLAIQLVAGLSVTLHPSTENSKAITAVATAEELLRTPKQEAVVSTWLQLSDGSATPLDIYDTKDFTLTATSLNEAVVSTPQARSPRWPVVMAEGEGQGPLVRVDMSIAEACQKSKRKSVLAVGVGSVRVKFGQGNADSSRGADGDSGEIKNHASDRRQKSQEPEGHLQGSPGEREDGALQRGDSTARPLLDNRVVKSGRPDAGRPSGGDQLQNIPLDFANFPAQVELPRAGGGLGASDLVQTPRGLSDLEIGMYALLGVFCLAILVFLINCATFALRYRHKQVPLEGQASVTHSHDWVWLGNEAELLEHAGEGSPPQDEHTTVLDRGPGGSDDGSRLLLNGGARQHVQGQVHRAGSAGRPARDPKLEPLHSPTSKRKKVKFTTFTTIPPDDGCPTVNSILGGGGGEDIKWVCQDVSPGAPKELRNYLEKFKDPA

Target ORF information:

RefSeq Version	XM_022410148.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris transmembrane protein 132C (TMEM132C), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022410148.1

ORF Insert Sequence:

ATGACCGTGA TCGAGGGCCG GGGGATCACA GATAATGTGC CACGGTTCTC TTCTCTGCCA 
CCCTTCCTGC CTGTGAGCTA CCACATCCTT GGAGCAGAGA CCTCCTTCTT CCTGAAGGAG 
GCCAACCAGG ACGTCCTGCG CAACTCCAGC CTGCACACCA GGGTGGAGTC CTTCTTCACC 
TACAAGGCCA ACCGGCCCCC AGTGCTCAAC GCCAGCTACG GGCCCTTCTC CATCGAGCAG 
GTGGTGCCTC AGGACCTCCT GCTGCCCTCC AGCCCCTTCG GAGCCACCAG CAAGCTCTCC 
CTCAACTGGA GGCTGAGGGC GCACATCCTG CGGGACAAGG TGTACCTGAG CCGGCCCCGG 
GTGCAGGTGC TCTTCCACCT GCTGGGCCGG GACTGGGCGG CACAGAGCCC TGGCGAGCGG 
CTGCCCTGCC TGCGGCTGTT CGCCTTCCGG GAGACCCGGG AGGTGCGGGC CGGCTGCCGG 
CTGCAGGGCG CCCTGGGGCT GTGCGTGGCC GAACTGGAGC TGCTGGCCGC CTGGTTCGGG 
CCCCCCACCG TGGTGGCCGG GAGGAAGCGG GCGCCTGGGC CGCCCGAGGG GAGCCCCGTG 
GAGCTCTACT ACTCCGTGCA GCCGGGGGAT GCGCGCGGGG ACTGTGCGGG CGGCGGCGGC 
GACGTCAGGA AGGGCAACGC CATCCGGCCT GGGAAGGACG GGCTGGATGA GGCCGTGCCC 
CACCTGCAGA GGATCGGCGC CGTCAGCCTC TACCGGGCCC AGGACAGCAC CCAGCTCAGC 
GAGCTGCGTT TGGACAGCAA CGTGGTCATC TGGCTGCCCT CCCGGCCCGT CAAGCAAGGA 
GATGTGGTCA CCGCCTACGT CACCATCGCC AGCAACTCCA CTGTGGACCT TTTCATCCTG 
AGAGCCAAGG TGAAGAAGGG GGTGAACATC CTGGGCACTC AGACCAGCGA GCCCCGGCAG 
TGGGATGTCA AACAGGAGAT GGGGAATGGC GGAAAGCATG CTACCACCAC CGTGCTGTGC 
CAGCGCCTGG GGTCCAGCAC ACGTAACAGA AGCAGCAGCC TGTTCAGTGA GGTTATGCAG 
ATGAACTTTG AAATAGCCAG CTTCAGTAGC CTCTCGGGGA CTCAGCCCAT CGCCTGGCAG 
GTGGAATATC CACGGAGAGC GACCACCGAC ACCGCTGTGT CCGAGATCTT CATCAGCCAG 
AAGGACCTGG CTGGCATCGT TCCTCTAGCC ACGGACACGG AAATTCTGAA CACTGCCATC 
CTCACGGGAA AGACAGTGGT CCTGCCCATC AAGGTGGTCT CTGTGGAGGA GAACAGTGCC 
GTGATGGATA TCTCCGAGTC GGTGGAGTGC AAGTCCACAG ACGAGGATGT CATCAAGGTG 
TCTGAAAGAT GTGACTACGT CTTTGTCAAT GGCAAAGAGA TGAAGGGCAA GGTGGATGCG 
GTGGTGAACT TCACCTACCA GCACCTGAGT GCCTCCCTGC ACATCACCGT GTGGGTGCCC 
CGGCTACCCC TACAGATTGA GGTCTCTGAC ACAGAGCTGA GCCAGATCAA AGGCTGGAGG 
GTCCCCATTG TGTCCAGTAA GAGGCCCACT CGAGAGAGCG AGGATGAAGA CGAGGAGGAG 
CGGCGGGGCC GAGGCTGTGC CCTGCAGTAC CAGCACGCCA CGGTGCGGGT CCTCACCCAG 
TTCGTGTCGG AGGGCGCTGG CCCGTGGGGC CAGCCGAGCC ACCTTCTCAG TCCCGACTGG 
CAAGTCGACA TCACCCACCT GGTTGCAGAC TTCATGAAGC TGGAGGAACC TCACGTGGCC 
ACACTCCAGG ACAGCAGGAT CCTGGTCGGG CGGGAGGTTG GGATGACCAC CATCCAGGTG 
TTGTCTCCAC TCTCTGACTC CATCCTGGCA GAGAAGACGG TGACCGTGTT AGATGACAAG 
GTATCGGTGA CAGACTTGGC CATCCAGCTC GTGGCTGGGC TGTCTGTCAC CCTCCACCCC 
AGCACGGAGA ACAGCAAGGC CATCACTGCT GTGGCCACAG CTGAGGAGCT GCTGCGGACC 
CCCAAACAGG AGGCCGTGGT CAGCACTTGG CTCCAGCTCA GCGACGGCTC CGCCACCCCC 
CTGGACATCT ACGACACCAA GGACTTCACC CTGACCGCCA CCTCCCTGAA CGAGGCCGTC 
GTGTCCACCC CCCAGGCCCG CTCTCCCAGA TGGCCGGTGG TGATGGCCGA AGGGGAAGGC 
CAGGGGCCGC TGGTGCGAGT GGACATGTCC ATCGCCGAGG CCTGCCAGAA GTCCAAGCGC 
AAGAGCGTCC TGGCCGTGGG TGTGGGCAGC GTCAGGGTCA AGTTTGGCCA GGGCAACGCC 
GACTCCAGCC GCGGCGCGGA CGGCGACAGT GGCGAGATCA AGAACCACGC CAGCGACCGC 
CGGCAGAAGA GCCAGGAGCC CGAGGGGCAC CTCCAAGGCA GCCCGGGGGA GCGCGAGGAC 
GGCGCCCTGC AGAGAGGCGA CAGCACGGCC AGGCCGCTCC TGGACAACAG GGTGGTGAAG 
AGCGGCCGGC CGGACGCGGG CAGGCCGTCC GGGGGGGACC AGCTGCAGAA CATCCCCCTG 
GACTTCGCCA ACTTCCCGGC GCAGGTGGAG CTGCCCCGGG CGGGGGGCGG CCTGGGGGCC 
AGCGACCTGG TGCAGACGCC CCGGGGCCTG AGCGACCTGG AGATCGGCAT GTACGCCCTG 
CTCGGCGTCT TCTGCTTGGC CATCCTTGTC TTCCTCATCA ACTGCGCCAC CTTCGCCCTC 
AGGTACCGCC ACAAGCAGGT GCCCCTGGAA GGCCAGGCCT CTGTGACCCA CTCGCACGAC 
TGGGTGTGGC TGGGCAACGA GGCGGAGCTC CTGGAGCACG CGGGCGAGGG GTCGCCACCG 
CAGGACGAGC ACACGACTGT CCTGGACCGC GGGCCGGGCG GCAGCGACGA CGGCAGCCGG 
CTGCTGCTCA ACGGCGGCGC CCGGCAGCAC GTGCAGGGCC AGGTGCACCG GGCGGGCTCG 
GCGGGCAGGC CGGCCAGGGA CCCGAAGCTC GAGCCCCTGC ATTCGCCCAC CTCCAAAAGG 
AAGAAAGTGA AGTTCACCAC CTTCACCACC ATCCCGCCCG ACGACGGCTG CCCCACCGTG 
AACTCCATCC TGGGGGGCGG CGGCGGCGAG GACATCAAGT GGGTGTGCCA GGACGTGTCC 
CCGGGCGCCC CCAAGGAGCT CAGAAACTAC CTGGAGAAAT TCAAGGACCC GGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM132C ( XM_014107711.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963186.1 Canis lupus familiaris transmembrane protein 132C (TMEM132C), transcript variant X1, mRNA.

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CloneID	OCe43183
Clone ID Related Accession (Same CDS sequence)	XM_014107711.1
Accession Version	XM_014107711.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3267bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	transmembrane protein 132C isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726106.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGTTCTTAG TGGGATCTGG AGCTCATGAT CTTGAAGTGA TCGAGGGCCG GGGGATCACA 
GATAATGTGC CACGGTTCTC TTCTCTGCCA CCCTTCCTGC CTGTGAGCTA CCACATCCTT 
GGAGCAGAGA CCTCCTTCTT CCTGAAGGAG GCCAACCAGG ACGTCCTGCG CAACTCCAGC 
CTGCACACCA GGGTGGAGTC CTTCTTCACC TACAAGGCCA ACCGGCCCCC AGTGCTCAAC 
GCCAGCTACG GGCCCTTCTC CATCGAGCAG GTGGTGCCTC AGGACCTCCT GCTGCCCTCC 
AGCCCCTTCG GAGCCACCAG CAAGCTCTCC CTCAACTGGA GGCTGAGGGC GCACATCCTG 
CGGGACAAGG TGTACCTGAG CCGGCCCCGG GTGCAGGTGC TCTTCCACCT GCTGGGCCGG 
GACTGGGCGG CACAGAGCCC TGGCGAGCGG CTGCCCTGCC TGCGGCTGTT CGCCTTCCGG 
GAGACCCGGG AGGTGCGGGC CGGCTGCCGG CTGCAGGGCG CCCTGGGGCT GTGCGTGGCC 
GAACTGGAGC TGCTGGCCGC CTGGTTCGGG CCCCCCACCG TGGTGGCCGG GAGGAAGCGG 
GCGCCTGGGC CGCCCGAGGG GAGCCCCGTG GAGCTCTACT ACTCCGTGCA GCCGGGGGAT 
GCGCGCGGGG ACTGTGCGGG CGGCGGCGGC GACGTCAGGA AGGGCAACGC CATCCGGCCT 
GGGAAGGACG GGCTGGATGA GGCCGTGCCC CACCTGCAGA GGATCGGCGC CGTCAGCCTC 
TACCGGGCCC AGGACAGCAC CCAGCTCAGC GAGCTGCGTT TGGACAGCAA CGTGGTCATC 
TGGCTGCCCT CCCGGCCCGT CAAGCAAGGA GATGTGGTCA CCGCCTACGT CACCATCGCC 
AGCAACTCCA CTGTGGACCT TTTCATCCTG AGAGCCAAGG TGAAGAAGGG GGTGAACATC 
CTGGGCACTC AGACCAGCGA GCCCCGGCAG TGGGATGTCA AACAGGAGAT GGGGAATGGC 
GGAAAGCATG CTACCACCAC CGTGCTGTGC CAGCGCCTGG GGTCCAGCAC ACGTAACAGA 
AGCAGCAGCC TGTTCAGTGA GGTTATGCAG ATGAACTTTG AAATAGCCAG CTTCAGTAGC 
CTCTCGGGGA CTCAGCCCAT CGCCTGGCAG GTGGAATATC CACGGAGAGC GACCACCGAC 
ACCGCTGTGT CCGAGATCTT CATCAGCCAG AAGGACCTGG CTGGCATCGT TCCTCTAGCC 
ACGGACACGG AAATTCTGAA CACTGCCATC CTCACGGGAA AGACAGTGGT CCTGCCCATC 
AAGGTGGTCT CTGTGGAGGA GAACAGTGCC GTGATGGATA TCTCCGAGTC GGTGGAGTGC 
AAGTCCACAG ACGAGGATGT CATCAAGGTG TCTGAAAGAT GTGACTACGT CTTTGTCAAT 
GGCAAAGAGA TGAAGGGCAA GGTGGATGCG GTGGTGAACT TCACCTACCA GCACCTGAGT 
GCCTCCCTGC ACATCACCGT GTGGGTGCCC CGGCTACCCC TACAGATTGA GGTCTCTGAC 
ACAGAGCTGA GCCAGATCAA AGGCTGGAGG GTCCCCATTG TGTCCAGTAA GAGGCCCACT 
CGAGAGAGCG AGGATGAAGA CGAGGAGGAG CGGCGGGGCC GAGGCTGTGC CCTGCAGTAC 
CAGCACGCCA CGGTGCGGGT CCTCACCCAG TTCGTGTCGG AGGGCGCTGG CCCGTGGGGC 
CAGCCGAGCC ACCTTCTCAG TCCCGACTGG CAAGTCGACA TCACCCACCT GGTTGCAGAC 
TTCATGAAGC TGGAGGAACC TCACGTGGCC ACACTCCAGG ACAGCAGGAT CCTGGTCGGG 
CGGGAGGTTG GGATGACCAC CATCCAGGTG TTGTCTCCAC TCTCTGACTC CATCCTGGCA 
GAGAAGACGG TGACCGTGTT AGATGACAAG GTATCGGTGA CAGACTTGGC CATCCAGCTC 
GTGGCTGGGC TGTCTGTCAC CCTCCACCCC AGCACGGAGA ACAGCAAGGC CATCACTGCT 
GTGGCCACAG CTGAGGAGCT GCTGCGGACC CCCAAACAGG AGGCCGTGGT CAGCACTTGG 
CTCCAGCTCA GCGACGGCTC CGCCACCCCC CTGGACATCT ACGACACCAA GGACTTCACC 
CTGACCGCCA CCTCCCTGAA CGAGGCCGTC GTGTCCACCC CCCAGGCCCG CTCTCCCAGA 
TGGCCGGTGG TGATGGCCGA AGGGGAAGGC CAGGGGCCGC TGGTGCGAGT GGACATGTCC 
ATCGCCGAGG CCTGCCAGAA GTCCAAGCGC AAGAGCGTCC TGGCCGTGGG TGTGGGCAGC 
GTCAGGGTCA AGTTTGGCCA GGGCAACGCC GACTCCAGCC GCGGCGCGGA CGGCGACAGT 
GGCGAGATCA AGAACCACGC CAGCGACCGC CGGCAGAAGA GCCAGGAGCC CGAGGGGCAC 
CTCCAAGGCA GCCCGGGGGA GCGCGAGGAC GGCGCCCTGC AGAGAGGCGA CAGCACGGCC 
AGGCCGCTCC TGGACAACAG GGTGGTGAAG AGCGGCCGGC CGGACGCGGG CAGGCCGTCC 
GGGGGGGACC AGCTGCAGAA CATCCCCCTG GACTTCGCCA ACTTCCCGGC GCAGGTGGAG 
CTGCCCCGGG CGGGGGGCGG CCTGGGGGCC AGCGACCTGG TGCAGACGCC CCGGGGCCTG 
AGCGACCTGG AGATCGGCAT GTACGCCCTG CTCGGCGTCT TCTGCTTGGC CATCCTTGTC 
TTCCTCATCA ACTGCGCCAC CTTCGCCCTC AGGTACCGCC ACAAGCAGGT GCCCCTGGAA 
GGCCAGGCCT CTGTGACCCA CTCGCACGAC TGGGTGTGGC TGGGCAACGA GGCGGAGCTC 
CTGGAGCACG CGGGCGAGGG GTCGCCACCG CAGGACGAGC ACACGACTGT CCTGGACCGC 
GGGCCGGGCG GCAGCGACGA CGGCAGCCGG CTGCTGCTCA ACGGCGGCGC CCGGCAGCAC 
GTGCAGGGCC AGGTGCACCG GGCGGGCTCG GCGGGCAGGC CGGCCAGGGA CCCGAAGCTC 
GAGCCCCTGC ATTCGCCCAC CTCCAAAAGG AAGAAAGTGA AGTTCACCAC CTTCACCACC 
ATCCCGCCCG ACGACGGCTG CCCCACCGTG AACTCCATCC TGGGGGGCGG CGGCGGCGAG 
GACATCAAGT GGGTGTGCCA GGACGTGTCC CCGGGCGCCC CCAAGGAGCT CAGAAACTAC 
CTGGAGAAAT TCAAGGACCC GGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963186.1
CDS	1..3267
Translation	MFLVGSGAHDLEVIEGRGITDNVPRFSSLPPFLPVSYHILGAETSFFLKEANQDVLRNSSLHTRVESFFTYKANRPPVLNASYGPFSIEQVVPQDLLLPSSPFGATSKLSLNWRLRAHILRDKVYLSRPRVQVLFHLLGRDWAAQSPGERLPCLRLFAFRETREVRAGCRLQGALGLCVAELELLAAWFGPPTVVAGRKRAPGPPEGSPVELYYSVQPGDARGDCAGGGGDVRKGNAIRPGKDGLDEAVPHLQRIGAVSLYRAQDSTQLSELRLDSNVVIWLPSRPVKQGDVVTAYVTIASNSTVDLFILRAKVKKGVNILGTQTSEPRQWDVKQEMGNGGKHATTTVLCQRLGSSTRNRSSSLFSEVMQMNFEIASFSSLSGTQPIAWQVEYPRRATTDTAVSEIFISQKDLAGIVPLATDTEILNTAILTGKTVVLPIKVVSVEENSAVMDISESVECKSTDEDVIKVSERCDYVFVNGKEMKGKVDAVVNFTYQHLSASLHITVWVPRLPLQIEVSDTELSQIKGWRVPIVSSKRPTRESEDEDEEERRGRGCALQYQHATVRVLTQFVSEGAGPWGQPSHLLSPDWQVDITHLVADFMKLEEPHVATLQDSRILVGREVGMTTIQVLSPLSDSILAEKTVTVLDDKVSVTDLAIQLVAGLSVTLHPSTENSKAITAVATAEELLRTPKQEAVVSTWLQLSDGSATPLDIYDTKDFTLTATSLNEAVVSTPQARSPRWPVVMAEGEGQGPLVRVDMSIAEACQKSKRKSVLAVGVGSVRVKFGQGNADSSRGADGDSGEIKNHASDRRQKSQEPEGHLQGSPGEREDGALQRGDSTARPLLDNRVVKSGRPDAGRPSGGDQLQNIPLDFANFPAQVELPRAGGGLGASDLVQTPRGLSDLEIGMYALLGVFCLAILVFLINCATFALRYRHKQVPLEGQASVTHSHDWVWLGNEAELLEHAGEGSPPQDEHTTVLDRGPGGSDDGSRLLLNGGARQHVQGQVHRAGSAGRPARDPKLEPLHSPTSKRKKVKFTTFTTIPPDDGCPTVNSILGGGGGEDIKWVCQDVSPGAPKELRNYLEKFKDPA

Target ORF information:

RefSeq Version	XM_014107711.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris transmembrane protein 132C (TMEM132C), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107711.1

ORF Insert Sequence:

ATGTTCTTAG TGGGATCTGG AGCTCATGAT CTTGAAGTGA TCGAGGGCCG GGGGATCACA 
GATAATGTGC CACGGTTCTC TTCTCTGCCA CCCTTCCTGC CTGTGAGCTA CCACATCCTT 
GGAGCAGAGA CCTCCTTCTT CCTGAAGGAG GCCAACCAGG ACGTCCTGCG CAACTCCAGC 
CTGCACACCA GGGTGGAGTC CTTCTTCACC TACAAGGCCA ACCGGCCCCC AGTGCTCAAC 
GCCAGCTACG GGCCCTTCTC CATCGAGCAG GTGGTGCCTC AGGACCTCCT GCTGCCCTCC 
AGCCCCTTCG GAGCCACCAG CAAGCTCTCC CTCAACTGGA GGCTGAGGGC GCACATCCTG 
CGGGACAAGG TGTACCTGAG CCGGCCCCGG GTGCAGGTGC TCTTCCACCT GCTGGGCCGG 
GACTGGGCGG CACAGAGCCC TGGCGAGCGG CTGCCCTGCC TGCGGCTGTT CGCCTTCCGG 
GAGACCCGGG AGGTGCGGGC CGGCTGCCGG CTGCAGGGCG CCCTGGGGCT GTGCGTGGCC 
GAACTGGAGC TGCTGGCCGC CTGGTTCGGG CCCCCCACCG TGGTGGCCGG GAGGAAGCGG 
GCGCCTGGGC CGCCCGAGGG GAGCCCCGTG GAGCTCTACT ACTCCGTGCA GCCGGGGGAT 
GCGCGCGGGG ACTGTGCGGG CGGCGGCGGC GACGTCAGGA AGGGCAACGC CATCCGGCCT 
GGGAAGGACG GGCTGGATGA GGCCGTGCCC CACCTGCAGA GGATCGGCGC CGTCAGCCTC 
TACCGGGCCC AGGACAGCAC CCAGCTCAGC GAGCTGCGTT TGGACAGCAA CGTGGTCATC 
TGGCTGCCCT CCCGGCCCGT CAAGCAAGGA GATGTGGTCA CCGCCTACGT CACCATCGCC 
AGCAACTCCA CTGTGGACCT TTTCATCCTG AGAGCCAAGG TGAAGAAGGG GGTGAACATC 
CTGGGCACTC AGACCAGCGA GCCCCGGCAG TGGGATGTCA AACAGGAGAT GGGGAATGGC 
GGAAAGCATG CTACCACCAC CGTGCTGTGC CAGCGCCTGG GGTCCAGCAC ACGTAACAGA 
AGCAGCAGCC TGTTCAGTGA GGTTATGCAG ATGAACTTTG AAATAGCCAG CTTCAGTAGC 
CTCTCGGGGA CTCAGCCCAT CGCCTGGCAG GTGGAATATC CACGGAGAGC GACCACCGAC 
ACCGCTGTGT CCGAGATCTT CATCAGCCAG AAGGACCTGG CTGGCATCGT TCCTCTAGCC 
ACGGACACGG AAATTCTGAA CACTGCCATC CTCACGGGAA AGACAGTGGT CCTGCCCATC 
AAGGTGGTCT CTGTGGAGGA GAACAGTGCC GTGATGGATA TCTCCGAGTC GGTGGAGTGC 
AAGTCCACAG ACGAGGATGT CATCAAGGTG TCTGAAAGAT GTGACTACGT CTTTGTCAAT 
GGCAAAGAGA TGAAGGGCAA GGTGGATGCG GTGGTGAACT TCACCTACCA GCACCTGAGT 
GCCTCCCTGC ACATCACCGT GTGGGTGCCC CGGCTACCCC TACAGATTGA GGTCTCTGAC 
ACAGAGCTGA GCCAGATCAA AGGCTGGAGG GTCCCCATTG TGTCCAGTAA GAGGCCCACT 
CGAGAGAGCG AGGATGAAGA CGAGGAGGAG CGGCGGGGCC GAGGCTGTGC CCTGCAGTAC 
CAGCACGCCA CGGTGCGGGT CCTCACCCAG TTCGTGTCGG AGGGCGCTGG CCCGTGGGGC 
CAGCCGAGCC ACCTTCTCAG TCCCGACTGG CAAGTCGACA TCACCCACCT GGTTGCAGAC 
TTCATGAAGC TGGAGGAACC TCACGTGGCC ACACTCCAGG ACAGCAGGAT CCTGGTCGGG 
CGGGAGGTTG GGATGACCAC CATCCAGGTG TTGTCTCCAC TCTCTGACTC CATCCTGGCA 
GAGAAGACGG TGACCGTGTT AGATGACAAG GTATCGGTGA CAGACTTGGC CATCCAGCTC 
GTGGCTGGGC TGTCTGTCAC CCTCCACCCC AGCACGGAGA ACAGCAAGGC CATCACTGCT 
GTGGCCACAG CTGAGGAGCT GCTGCGGACC CCCAAACAGG AGGCCGTGGT CAGCACTTGG 
CTCCAGCTCA GCGACGGCTC CGCCACCCCC CTGGACATCT ACGACACCAA GGACTTCACC 
CTGACCGCCA CCTCCCTGAA CGAGGCCGTC GTGTCCACCC CCCAGGCCCG CTCTCCCAGA 
TGGCCGGTGG TGATGGCCGA AGGGGAAGGC CAGGGGCCGC TGGTGCGAGT GGACATGTCC 
ATCGCCGAGG CCTGCCAGAA GTCCAAGCGC AAGAGCGTCC TGGCCGTGGG TGTGGGCAGC 
GTCAGGGTCA AGTTTGGCCA GGGCAACGCC GACTCCAGCC GCGGCGCGGA CGGCGACAGT 
GGCGAGATCA AGAACCACGC CAGCGACCGC CGGCAGAAGA GCCAGGAGCC CGAGGGGCAC 
CTCCAAGGCA GCCCGGGGGA GCGCGAGGAC GGCGCCCTGC AGAGAGGCGA CAGCACGGCC 
AGGCCGCTCC TGGACAACAG GGTGGTGAAG AGCGGCCGGC CGGACGCGGG CAGGCCGTCC 
GGGGGGGACC AGCTGCAGAA CATCCCCCTG GACTTCGCCA ACTTCCCGGC GCAGGTGGAG 
CTGCCCCGGG CGGGGGGCGG CCTGGGGGCC AGCGACCTGG TGCAGACGCC CCGGGGCCTG 
AGCGACCTGG AGATCGGCAT GTACGCCCTG CTCGGCGTCT TCTGCTTGGC CATCCTTGTC 
TTCCTCATCA ACTGCGCCAC CTTCGCCCTC AGGTACCGCC ACAAGCAGGT GCCCCTGGAA 
GGCCAGGCCT CTGTGACCCA CTCGCACGAC TGGGTGTGGC TGGGCAACGA GGCGGAGCTC 
CTGGAGCACG CGGGCGAGGG GTCGCCACCG CAGGACGAGC ACACGACTGT CCTGGACCGC 
GGGCCGGGCG GCAGCGACGA CGGCAGCCGG CTGCTGCTCA ACGGCGGCGC CCGGCAGCAC 
GTGCAGGGCC AGGTGCACCG GGCGGGCTCG GCGGGCAGGC CGGCCAGGGA CCCGAAGCTC 
GAGCCCCTGC ATTCGCCCAC CTCCAAAAGG AAGAAAGTGA AGTTCACCAC CTTCACCACC 
ATCCCGCCCG ACGACGGCTG CCCCACCGTG AACTCCATCC TGGGGGGCGG CGGCGGCGAG 
GACATCAAGT GGGTGTGCCA GGACGTGTCC CCGGGCGCCC CCAAGGAGCT CAGAAACTAC 
CTGGAGAAAT TCAAGGACCC GGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A method for the large-scale cloning of nuclear proteins and nuclear targeting sequences on a functional basis.
Analytical biochemistry284(2)231-9(2000 Sep)
Pichon B,Mercan D,Pouillon V,Christophe-Hobertus C,Christophe D

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TMEM132C cDNA ORF clone, Canis lupus familiaris(dog)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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