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KDM6A cDNA ORF clone, Canis lupus familiaris(dog)

Summary

Gene Symbol KDM6A
Entrez Gene ID 491845
Full Name lysine demethylase 6A
Synonyms UTX
Gene Type protein-coding
Organism Canis lupus familiaris(dog)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_005641005.3 XP_005641062.1 lysine-specific demethylase 6A isoform X2
XM_005641004.2 XP_005641061.1 lysine-specific demethylase 6A isoform X1
XM_005641007.2 XP_005641064.1 lysine-specific demethylase 6A isoform X4
NM_001197185.1 NP_001184114.1 lysine-specific demethylase 6A isoform 1
XM_005641004.3 XP_005641061.1 lysine-specific demethylase 6A isoform X1
XM_005641008.2 XP_005641065.1 lysine-specific demethylase 6A isoform X5
XM_005641005.2 XP_005641062.1 lysine-specific demethylase 6A isoform X2
XM_005641006.2 XP_005641063.1 lysine-specific demethylase 6A isoform X3
XM_005641006.3 XP_005641063.1 lysine-specific demethylase 6A isoform X3
NM_001197186.1 NP_001184115.1 lysine-specific demethylase 6A isoform 2

Pathways from BioSystems


Homologies


Homo sapiens (human) KDM6A NP_066963.2
Danio rerio (zebrafish) kdm6a XP_697746.2
Anopheles gambiae (African malaria mosquito) AgaP_AGAP008509 XP_316935.4
Xenopus tropicalis (tropical clawed frog) kdm6a NP_001123807.1
Bos taurus (cattle) KDM6A NP_001193504.1
Drosophila melanogaster (fruit fly) Utx NP_001188773.1
Pan troglodytes (chimpanzee) KDM6A XP_003954022.1
Canis lupus familiaris (dog) KDM6A NP_001184114.1
Caenorhabditis elegans (roundworm) utx-1 NP_509450.1
Rattus norvegicus (Norway rat) Kdm6a XP_006256701.1
KDM6A XP_002806253.1
Mus musculus (house mouse) Kdm6a NP_033509.1
Gallus gallus (chicken) KDM6A XP_416762.4

Gene Ontology

The following KDM6A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KDM6A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe31953 XM_005641008.2
Latest version!		 Canis lupus familiaris lysine (K)-specific demethylase 6A (KDM6A), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $713.30
$1019.00
OCe34168 NM_001197185.1
Latest version!		 Canis lupus familiaris lysine demethylase 6A (KDM6A), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $797.30
$1139.00
OCe34172 NM_001197186.1
Latest version!		 Canis lupus familiaris lysine demethylase 6A (KDM6A), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $797.30
$1139.00
OCe31949 XM_005641004.3
Latest version!		 Canis lupus familiaris lysine demethylase 6A (KDM6A), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $797.30
$1139.00
OCe31950 XM_005641005.3
Latest version!		 Canis lupus familiaris lysine demethylase 6A (KDM6A), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $797.30
$1139.00
OCe31951 XM_005641006.3
Latest version!		 Canis lupus familiaris lysine demethylase 6A (KDM6A), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $797.30
$1139.00
OCe31952 XM_005641007.2
Latest version!		 Canis lupus familiaris lysine (K)-specific demethylase 6A (KDM6A), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
OCe31949 XM_005641004.2 Canis lupus familiaris lysine (K)-specific demethylase 6A (KDM6A), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $797.30
$1139.00
OCe31950 XM_005641005.2 Canis lupus familiaris lysine (K)-specific demethylase 6A (KDM6A), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $797.30
$1139.00
OCe31951 XM_005641006.2 Canis lupus familiaris lysine (K)-specific demethylase 6A (KDM6A), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $797.30
$1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OCe31953
Clone ID Related Accession (Same CDS sequence) XM_005641008.2
Accession Version XM_005641008.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3969bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2015-09-16
Organism Canis lupus familiaris(Dog)
Product lysine-specific demethylase 6A isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005641008.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
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181
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361
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481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTATT CGGGTTCGTG 
AGGTTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAC 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAAT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTACA ATGCATTTCA CTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAT 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG CAATCCATGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAG 
TATCATTCTG CAAAGGAAGC TTATGAACAA CTTTTGCAGA CGGAAAACCT TTCTGCACAA 
GTAAAGGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTTCTGGGA 
GATAAAGCTA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTT GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGCGTGCT ATATCAGCAG CAAAATCAGC CTATGGATGC TTTACAGGCT 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACA 
CTCTACGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACCAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGAATACT 
TCTGATAATT GGAGTAGTGG CCATGCAGTG TCACATCCTC CAGTACAGCA ACAAGCTCAT 
TCGTGGTGTT TGACACCACA GAAATTACAG ATGAGACAAA CAGGAGTTGC ACAGGTACGA 
TCTACTGGAA TTCCTAATGG GCCAACAGCT GACTCATCAC TGCCTACAAA CTCAGTCTCT 
GGCCAGCAGC CACAGCTTGC TCTGACCAGA GTGCCTAGCG TCTCTCAGCC TGGAGTCCGC 
CCTGCCTGCC CTGGGCAGCC TTTGGCCAAC GGACCCTTTT CTGCAGGCCA TGTTCCCTGT 
AGCACATCAA GAACGCTGGG GAGTACAGAC ACTATATTGA TAGGCAATAA TCACATAACA 
GGAAGTGGAA GTAATGGAAA CGTGCCTTAC CTGCAGCGAA ACGCACTCAC TCTACCTCAT 
AACCGCACAA ACCTGACCAG CAGCGCAGAG GAGCCGTGGA AAAACCAACT ATCTAACTCC 
ACTCAGGGGC TTCACAAAGG TCAGAGTTCA CATTTGGCAG GTCCTAATGG TGAACGACCT 
CTCTCTTCCA CTGGGCCTTC CCAGCATCTC CAGGCAGCTG GCTCTGGTAT TCAGAATCAG 
AATGGACATC CCACCCTGCC TAGCAATTCA GTAACACAGG GGGCTGCTCT CAATCACCTC 
TCCTCTCACA CTGCTACCTC AGGTGGACAA CAAGGCATTA CCTTAACCAA AGAGAGCAAG 
CCTTCAGGAA ACACATCGAT GGTGCCTGAA ACAAGCAGGC ACGCTGGAGA GACACCTAAC 
AGCACTGCCA GTGTCGAGGG ACTTCCTAAT CATGTCCATC AGGTGACGGC AGATGCTGTT 
TGCAGTCCTA GCCATGGAGA TTCTAAGTCA CCAGGTTTAC TAAGTTCAGA CAATCCTCAG 
CTCTCTGCCT TGTTGATGGG AAAAGCCAAT AACAATGTGG GTACTGGAAC CTGTGACAAA 
GTCAATAACA TCCACCCAGC TGTTCATACA AAGACTGATA ATTCTGTTGC CTCTTCACCA 
TCTTCAGCCA TTTCCACAGC AACACCTTCT CCAAAATCCA CTGAGCAGAC AACCACAAAC 
AGTGTTACCA GCCTTAATAG CCCTCACAGT GGGCTACACA CAATTAATGG AGAAGGAATG 
GAGGAATCTC AGAGCCCCAT GAAAACAGAT GTGCTTCTGA TTAGCCACAA ACCTAATCCT 
CAGATCATAC CATCAATGTC TGTATCCATA TACCCCAGCT CAGCAGAAGT TCTGAAGGCA 
TGCAGGAATC TAGGTAAAAA TGGCTTATCT AACAGTAGCA TTTTATTGGA TAAATGTCCG 
CCTCCAAGAC CACCATCTTC ACCATACCCT CCCTTGCCAA AGGACAAGTT GAATCCACCT 
ACACCTAGTA TTTATTTGGA AAATAAACGT GATGCTTTCT TTCCTCCATT ACACCAATTT 
TGTACAAATC CAAACAACCC TGTTACGGTA ATACGTGGCC TTGCTGGAGC TCTTAAGTTG 
GACCTGGGAC TTTTCTCTAC CAAAACTTTG GTGGAAGCTA ACAATGAACA TATGGTGGAA 
GTGAGGACAC AGTTGTTGCA ACCAGCAGAT GAAAACTGGG ATCCTACTGG AACAAAGAAA 
ATCTGGCATT GTGAAAGTAA TCGATCTCAT ACTACAATTG CTAAATATGC ACAGTACCAG 
GCCTCCTCCT TCCAGGAATC ATTGAGGGAA GAAAATGAGA AAAGAAGTCA CCATAAAGAC 
CATTCAGATA GTGAATCTAC ATCTTCAGAT AATTCTGGGA GGAGGAGGAA AGGACCCTTT 
AAAACCATAA AGTTTGGGAC CAACATTGAC CTGTCTGATG ACAAAAAATG GAAGTTGCAG 
CTACATGAGC TGACTAAACT TCCTGCTTTT GTGCGTGTCG TATCAGCAGG AAATCTTCTA 
AGCCACGTTG GTCATACCAT ACTGGGCATG AACACAGTTC AACTGTACAT GAAAGTTCCA 
GGAAGCAGAA CACCAGGTCA TCAAGAAAAT AACAACTTCT GTTCGGTTAA TATAAATATT 
GGTCCAGGTG ACTGTGAATG GTTTGTTGTC CCTGAAGGTT ACTGGGGTGT TCTGAATGAC 
TTCTGTGAAA AAAATAATTT GAATTTCCTA ATGGGTTCTT GGTGGCCCAA CCTTGAAGAT 
CTTTATGAAG CAAATGTTCC AGTGTATAGG TTTATTCAGC GACCTGGAGA TTTGGTCTGG 
ATAAATGCAG GCACTGTTCA TTGGGTTCAG GCTATTGGCT GGTGCAACAA CATTGCTTGG 
AATGTTGGTC CACTTACAGC CTGCCAGTAT AAATTGGCAG TGGAACGGTA CGAATGGAAC 
AAATTGCAAA GTGTGAAGTC AATAGTACCC ATGGTTCATC TTTCCTGGAA TATGGCACGA 
AATATCAAGG TCTCAGATCC AAAGCTTTTT GAAATGATTA AGTATTGTCT TCTGAGAACT 
CTGAAGCAAT GTCAGACATT GAGGGAAGCT CTCATTGCTG CAGGAAAAGA GATTATATGG 
CATGGGCGGA CAAAAGAAGA ACCAGCGCAT TACTGTAGCA TTTGTGAGGT GGAGGTTTTT 
GATCTGCTTT TTGTCACAAA TGAGAGCAAT TCTCGAAAGA CCTACATAGT GCATTGCCAA 
GACTGTGCAC GAAAAACAAG TGGAAACCTG GAAAATTTTG TGGTGCTAGA ACAGTATAAA 
ATGGAGGACC TGATGCAAGT CTATGACCAA TTTACATTAG CTCCTCCATT ACCATCCTCC 
TCATCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005641065.1
CDS380..4348
Translation

Target ORF information:

RefSeq Version XM_005641008.2
Organism Canis lupus familiaris(Dog)
Definition Canis lupus familiaris lysine (K)-specific demethylase 6A (KDM6A), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005641008.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTATT CGGGTTCGTG 
AGGTTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAC 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAAT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTACA ATGCATTTCA CTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAT 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG CAATCCATGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAG 
TATCATTCTG CAAAGGAAGC TTATGAACAA CTTTTGCAGA CGGAAAACCT TTCTGCACAA 
GTAAAGGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTTCTGGGA 
GATAAAGCTA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTT GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGCGTGCT ATATCAGCAG CAAAATCAGC CTATGGATGC TTTACAGGCT 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACA 
CTCTACGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACCAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGAATACT 
TCTGATAATT GGAGTAGTGG CCATGCAGTG TCACATCCTC CAGTACAGCA ACAAGCTCAT 
TCGTGGTGTT TGACACCACA GAAATTACAG ATGAGACAAA CAGGAGTTGC ACAGGTACGA 
TCTACTGGAA TTCCTAATGG GCCAACAGCT GACTCATCAC TGCCTACAAA CTCAGTCTCT 
GGCCAGCAGC CACAGCTTGC TCTGACCAGA GTGCCTAGCG TCTCTCAGCC TGGAGTCCGC 
CCTGCCTGCC CTGGGCAGCC TTTGGCCAAC GGACCCTTTT CTGCAGGCCA TGTTCCCTGT 
AGCACATCAA GAACGCTGGG GAGTACAGAC ACTATATTGA TAGGCAATAA TCACATAACA 
GGAAGTGGAA GTAATGGAAA CGTGCCTTAC CTGCAGCGAA ACGCACTCAC TCTACCTCAT 
AACCGCACAA ACCTGACCAG CAGCGCAGAG GAGCCGTGGA AAAACCAACT ATCTAACTCC 
ACTCAGGGGC TTCACAAAGG TCAGAGTTCA CATTTGGCAG GTCCTAATGG TGAACGACCT 
CTCTCTTCCA CTGGGCCTTC CCAGCATCTC CAGGCAGCTG GCTCTGGTAT TCAGAATCAG 
AATGGACATC CCACCCTGCC TAGCAATTCA GTAACACAGG GGGCTGCTCT CAATCACCTC 
TCCTCTCACA CTGCTACCTC AGGTGGACAA CAAGGCATTA CCTTAACCAA AGAGAGCAAG 
CCTTCAGGAA ACACATCGAT GGTGCCTGAA ACAAGCAGGC ACGCTGGAGA GACACCTAAC 
AGCACTGCCA GTGTCGAGGG ACTTCCTAAT CATGTCCATC AGGTGACGGC AGATGCTGTT 
TGCAGTCCTA GCCATGGAGA TTCTAAGTCA CCAGGTTTAC TAAGTTCAGA CAATCCTCAG 
CTCTCTGCCT TGTTGATGGG AAAAGCCAAT AACAATGTGG GTACTGGAAC CTGTGACAAA 
GTCAATAACA TCCACCCAGC TGTTCATACA AAGACTGATA ATTCTGTTGC CTCTTCACCA 
TCTTCAGCCA TTTCCACAGC AACACCTTCT CCAAAATCCA CTGAGCAGAC AACCACAAAC 
AGTGTTACCA GCCTTAATAG CCCTCACAGT GGGCTACACA CAATTAATGG AGAAGGAATG 
GAGGAATCTC AGAGCCCCAT GAAAACAGAT GTGCTTCTGA TTAGCCACAA ACCTAATCCT 
CAGATCATAC CATCAATGTC TGTATCCATA TACCCCAGCT CAGCAGAAGT TCTGAAGGCA 
TGCAGGAATC TAGGTAAAAA TGGCTTATCT AACAGTAGCA TTTTATTGGA TAAATGTCCG 
CCTCCAAGAC CACCATCTTC ACCATACCCT CCCTTGCCAA AGGACAAGTT GAATCCACCT 
ACACCTAGTA TTTATTTGGA AAATAAACGT GATGCTTTCT TTCCTCCATT ACACCAATTT 
TGTACAAATC CAAACAACCC TGTTACGGTA ATACGTGGCC TTGCTGGAGC TCTTAAGTTG 
GACCTGGGAC TTTTCTCTAC CAAAACTTTG GTGGAAGCTA ACAATGAACA TATGGTGGAA 
GTGAGGACAC AGTTGTTGCA ACCAGCAGAT GAAAACTGGG ATCCTACTGG AACAAAGAAA 
ATCTGGCATT GTGAAAGTAA TCGATCTCAT ACTACAATTG CTAAATATGC ACAGTACCAG 
GCCTCCTCCT TCCAGGAATC ATTGAGGGAA GAAAATGAGA AAAGAAGTCA CCATAAAGAC 
CATTCAGATA GTGAATCTAC ATCTTCAGAT AATTCTGGGA GGAGGAGGAA AGGACCCTTT 
AAAACCATAA AGTTTGGGAC CAACATTGAC CTGTCTGATG ACAAAAAATG GAAGTTGCAG 
CTACATGAGC TGACTAAACT TCCTGCTTTT GTGCGTGTCG TATCAGCAGG AAATCTTCTA 
AGCCACGTTG GTCATACCAT ACTGGGCATG AACACAGTTC AACTGTACAT GAAAGTTCCA 
GGAAGCAGAA CACCAGGTCA TCAAGAAAAT AACAACTTCT GTTCGGTTAA TATAAATATT 
GGTCCAGGTG ACTGTGAATG GTTTGTTGTC CCTGAAGGTT ACTGGGGTGT TCTGAATGAC 
TTCTGTGAAA AAAATAATTT GAATTTCCTA ATGGGTTCTT GGTGGCCCAA CCTTGAAGAT 
CTTTATGAAG CAAATGTTCC AGTGTATAGG TTTATTCAGC GACCTGGAGA TTTGGTCTGG 
ATAAATGCAG GCACTGTTCA TTGGGTTCAG GCTATTGGCT GGTGCAACAA CATTGCTTGG 
AATGTTGGTC CACTTACAGC CTGCCAGTAT AAATTGGCAG TGGAACGGTA CGAATGGAAC 
AAATTGCAAA GTGTGAAGTC AATAGTACCC ATGGTTCATC TTTCCTGGAA TATGGCACGA 
AATATCAAGG TCTCAGATCC AAAGCTTTTT GAAATGATTA AGTATTGTCT TCTGAGAACT 
CTGAAGCAAT GTCAGACATT GAGGGAAGCT CTCATTGCTG CAGGAAAAGA GATTATATGG 
CATGGGCGGA CAAAAGAAGA ACCAGCGCAT TACTGTAGCA TTTGTGAGGT GGAGGTTTTT 
GATCTGCTTT TTGTCACAAA TGAGAGCAAT TCTCGAAAGA CCTACATAGT GCATTGCCAA 
GACTGTGCAC GAAAAACAAG TGGAAACCTG GAAAATTTTG TGGTGCTAGA ACAGTATAAA 
ATGGAGGACC TGATGCAAGT CTATGACCAA TTTACATTAG CTCCTCCATT ACCATCCTCC 
TCATCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe34168
Clone ID Related Accession (Same CDS sequence) NM_001197185.1
Accession Version NM_001197185.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 4260bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-04-14
Organism Canis lupus familiaris(dog)
Product lysine-specific demethylase 6A isoform 1
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAEX03026306.1, AY530157.1, AY530158.1 and CF412223.1. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments, including orthologous human transcript (NM_021140.2). ##Evidence-Data-START## Transcript exon combination :: AY530157.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA103936001, SAMEA103936002 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTATT CGGGTTCGTG 
AGGTTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAC 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAAT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTACA ATGCATTTCA CTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAT 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG CAATCCATGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAG 
TATCATTCTG CAAAGGAAGC TTATGAACAA CTTTTGCAGA CGGAAAACCT TTCTGCACAA 
GTAAAGGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTTCTGGGA 
GATAAAGCTA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTT GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGCGTGCT ATATCAGCAG CAAAATCAGC CTATGGATGC TTTACAGGCT 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACA 
CTCTACGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACCAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG 
TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG 
GAGGCGTGGA GCCTACCAAT CCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA 
GCACAGCAGG CATGTAAACC TCATCATCCA AATACTGAAC CTGTATTAGG CCTCAGTCAA 
ACACCAATTT CACAGCAGTC CTTGCCACTA CACATGATTC CTTCTAGCCA AGTAGATGAC 
CTGTCCAGTC CTGCCAAGAG GAAAAGAACA TCTAGTCCAA CAAAGAATAC TTCTGATAAT 
TGGAGTAGTG GCCATGCAGT GTCACATCCT CCAGTACAGC AACAAGCTCA TTCGTGGTGT 
TTGACACCAC AGAAATTACA GATGAGACAA ACAGGAGTTG CACAGGTACG ATCTACTGGA 
ATTCCTAATG GGCCAACAGC TGACTCATCA CTGCCTACAA ACTCAGTCTC TGGCCAGCAG 
CCACAGCTTG CTCTGACCAG AGTGCCTAGC GTCTCTCAGC CTGGAGTCCG CCCTGCCTGC 
CCTGGGCAGC CTTTGGCCAA CGGACCCTTT TCTGCAGGCC ATGTTCCCTG TAGCACATCA 
AGAACGCTGG GGAGTACAGA CACTATATTG ATAGGCAATA ATCACATAAC AGGAAGTGGA 
AGTAATGGAA ACGTGCCTTA CCTGCAGCGA AACGCACTCA CTCTACCTCA TAACCGCACA 
AACCTGACCA GCAGCGCAGA GGAGCCGTGG AAAAACCAAC TATCTAACTC CACTCAGGGG 
CTTCACAAAG GTCAGAGTTC ACATTTGGCA GGTCCTAATG GTGAACGACC TCTCTCTTCC 
ACTGGGCCTT CCCAGCATCT CCAGGCAGCT GGCTCTGGTA TTCAGAATCA GAATGGACAT 
CCCACCCTGC CTAGCAATTC AGTAACACAG GGGGCTGCTC TCAATCACCT CTCCTCTCAC 
ACTGCTACCT CAGGTGGACA ACAAGGCATT ACCTTAACCA AAGAGAGCAA GCCTTCAGGA 
AACACATCGA TGGTGCCTGA AACAAGCAGG CACGCTGGAG AGACACCTAA CAGCACTGCC 
AGTGTCGAGG GACTTCCTAA TCATGTCCAT CAGGTGACGG CAGATGCTGT TTGCAGTCCT 
AGCCATGGAG ATTCTAAGTC ACCAGGTTTA CTAAGTTCAG ACAATCCTCA GCTCTCTGCC 
TTGTTGATGG GAAAAGCCAA TAACAATGTG GGTACTGGAA CCTGTGACAA AGTCAATAAC 
ATCCACCCAG CTGTTCATAC AAAGACTGAT AATTCTGTTG CCTCTTCACC ATCTTCAGCC 
ATTTCCACAG CAACACCTTC TCCAAAATCC ACTGAGCAGA CAACCACAAA CAGTGTTACC 
AGCCTTAATA GCCCTCACAG TGGGCTACAC ACAATTAATG GAGAAGGAAT GGAGGAATCT 
CAGAGCCCCA TGAAAACAGA TGTGCTTCTG ATTAGCCACA AACCTAATCC TCAGATCATA 
CCATCAATGT CTGTATCCAT ATACCCCAGC TCAGCAGAAG TTCTGAAGGC ATGCAGGAAT 
CTAGGTAAAA ATGGCTTATC TAACAGTAGC ATTTTATTGG ATAAATGTCC GCCTCCAAGA 
CCACCATCTT CACCATACCC TCCCTTGCCA AAGGACAAGT TGAATCCACC TACACCTAGT 
ATTTATTTGG AAAATAAACG TGATGCTTTC TTTCCTCCAT TACACCAATT TTGTACAAAT 
CCAAACAACC CTGTTACGGT AATACGTGGC CTTGCTGGAG CTCTTAAGTT GGACCTGGGA 
CTTTTCTCTA CCAAAACTTT GGTGGAAGCT AACAATGAAC ATATGGTGGA AGTGAGGACA 
CAGTTGTTGC AACCAGCAGA TGAAAACTGG GATCCTACTG GAACAAAGAA AATCTGGCAT 
TGTGAAAGTA ATCGATCTCA TACTACAATT GCTAAATATG CACAGTACCA GGCCTCCTCC 
TTCCAGGAAT CATTGAGGGA AGAAAATGAG AAAAGAAGTC ACCATAAAGA CCATTCAGAT 
AGTGAATCTA CATCTTCAGA TAATTCTGGG AGGAGGAGGA AAGGACCCTT TAAAACCATA 
AAGTTTGGGA CCAACATTGA CCTGTCTGAT GACAAAAAAT GGAAGTTGCA GCTACATGAG 
CTGACTAAAC TTCCTGCTTT TGTGCGTGTC GTATCAGCAG GAAATCTTCT AAGCCACGTT 
GGTCATACCA TACTGGGCAT GAACACAGTT CAACTGTACA TGAAAGTTCC AGGAAGCAGA 
ACACCAGGTC ATCAAGAAAA TAACAACTTC TGTTCGGTTA ATATAAATAT TGGTCCAGGT 
GACTGTGAAT GGTTTGTTGT CCCTGAAGGT TACTGGGGTG TTCTGAATGA CTTCTGTGAA 
AAAAATAATT TGAATTTCCT AATGGGTTCT TGGTGGCCCA ACCTTGAAGA TCTTTATGAA 
GCAAATGTTC CAGTGTATAG GTTTATTCAG CGACCTGGAG ATTTGGTCTG GATAAATGCA 
GGCACTGTTC ATTGGGTTCA GGCTATTGGC TGGTGCAACA ACATTGCTTG GAATGTTGGT 
CCACTTACAG CCTGCCAGTA TAAATTGGCA GTGGAACGGT ACGAATGGAA CAAATTGCAA 
AGTGTGAAGT CAATAGTACC CATGGTTCAT CTTTCCTGGA ATATGGCACG AAATATCAAG 
GTCTCAGATC CAAAGCTTTT TGAAATGATT AAGTATTGTC TTCTGAGAAC TCTGAAGCAA 
TGTCAGACAT TGAGGGAAGC TCTCATTGCT GCAGGAAAAG AGATTATATG GCATGGGCGG 
ACAAAAGAAG AACCAGCGCA TTACTGTAGC ATTTGTGAGG TGGAGGTTTT TGATCTGCTT 
TTTGTCACAA ATGAGAGCAA TTCTCGAAAG ACCTACATAG TGCATTGCCA AGACTGTGCA 
CGAAAAACAA GTGGAAACCT GGAAAATTTT GTGGTGCTAG AACAGTATAA AATGGAGGAC 
CTGATGCAAG TCTATGACCA ATTTACATTA GCTCCTCCAT TACCATCCTC CTCATCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001184114.1
CDS371..4630
Translation

Target ORF information:

RefSeq Version NM_001197185.1
Organism Canis lupus familiaris(dog)
Definition Canis lupus familiaris lysine demethylase 6A (KDM6A), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001197185.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTATT CGGGTTCGTG 
AGGTTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAC 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAAT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTACA ATGCATTTCA CTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAT 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG CAATCCATGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAG 
TATCATTCTG CAAAGGAAGC TTATGAACAA CTTTTGCAGA CGGAAAACCT TTCTGCACAA 
GTAAAGGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTTCTGGGA 
GATAAAGCTA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTT GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGCGTGCT ATATCAGCAG CAAAATCAGC CTATGGATGC TTTACAGGCT 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACA 
CTCTACGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACCAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG 
TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG 
GAGGCGTGGA GCCTACCAAT CCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA 
GCACAGCAGG CATGTAAACC TCATCATCCA AATACTGAAC CTGTATTAGG CCTCAGTCAA 
ACACCAATTT CACAGCAGTC CTTGCCACTA CACATGATTC CTTCTAGCCA AGTAGATGAC 
CTGTCCAGTC CTGCCAAGAG GAAAAGAACA TCTAGTCCAA CAAAGAATAC TTCTGATAAT 
TGGAGTAGTG GCCATGCAGT GTCACATCCT CCAGTACAGC AACAAGCTCA TTCGTGGTGT 
TTGACACCAC AGAAATTACA GATGAGACAA ACAGGAGTTG CACAGGTACG ATCTACTGGA 
ATTCCTAATG GGCCAACAGC TGACTCATCA CTGCCTACAA ACTCAGTCTC TGGCCAGCAG 
CCACAGCTTG CTCTGACCAG AGTGCCTAGC GTCTCTCAGC CTGGAGTCCG CCCTGCCTGC 
CCTGGGCAGC CTTTGGCCAA CGGACCCTTT TCTGCAGGCC ATGTTCCCTG TAGCACATCA 
AGAACGCTGG GGAGTACAGA CACTATATTG ATAGGCAATA ATCACATAAC AGGAAGTGGA 
AGTAATGGAA ACGTGCCTTA CCTGCAGCGA AACGCACTCA CTCTACCTCA TAACCGCACA 
AACCTGACCA GCAGCGCAGA GGAGCCGTGG AAAAACCAAC TATCTAACTC CACTCAGGGG 
CTTCACAAAG GTCAGAGTTC ACATTTGGCA GGTCCTAATG GTGAACGACC TCTCTCTTCC 
ACTGGGCCTT CCCAGCATCT CCAGGCAGCT GGCTCTGGTA TTCAGAATCA GAATGGACAT 
CCCACCCTGC CTAGCAATTC AGTAACACAG GGGGCTGCTC TCAATCACCT CTCCTCTCAC 
ACTGCTACCT CAGGTGGACA ACAAGGCATT ACCTTAACCA AAGAGAGCAA GCCTTCAGGA 
AACACATCGA TGGTGCCTGA AACAAGCAGG CACGCTGGAG AGACACCTAA CAGCACTGCC 
AGTGTCGAGG GACTTCCTAA TCATGTCCAT CAGGTGACGG CAGATGCTGT TTGCAGTCCT 
AGCCATGGAG ATTCTAAGTC ACCAGGTTTA CTAAGTTCAG ACAATCCTCA GCTCTCTGCC 
TTGTTGATGG GAAAAGCCAA TAACAATGTG GGTACTGGAA CCTGTGACAA AGTCAATAAC 
ATCCACCCAG CTGTTCATAC AAAGACTGAT AATTCTGTTG CCTCTTCACC ATCTTCAGCC 
ATTTCCACAG CAACACCTTC TCCAAAATCC ACTGAGCAGA CAACCACAAA CAGTGTTACC 
AGCCTTAATA GCCCTCACAG TGGGCTACAC ACAATTAATG GAGAAGGAAT GGAGGAATCT 
CAGAGCCCCA TGAAAACAGA TGTGCTTCTG ATTAGCCACA AACCTAATCC TCAGATCATA 
CCATCAATGT CTGTATCCAT ATACCCCAGC TCAGCAGAAG TTCTGAAGGC ATGCAGGAAT 
CTAGGTAAAA ATGGCTTATC TAACAGTAGC ATTTTATTGG ATAAATGTCC GCCTCCAAGA 
CCACCATCTT CACCATACCC TCCCTTGCCA AAGGACAAGT TGAATCCACC TACACCTAGT 
ATTTATTTGG AAAATAAACG TGATGCTTTC TTTCCTCCAT TACACCAATT TTGTACAAAT 
CCAAACAACC CTGTTACGGT AATACGTGGC CTTGCTGGAG CTCTTAAGTT GGACCTGGGA 
CTTTTCTCTA CCAAAACTTT GGTGGAAGCT AACAATGAAC ATATGGTGGA AGTGAGGACA 
CAGTTGTTGC AACCAGCAGA TGAAAACTGG GATCCTACTG GAACAAAGAA AATCTGGCAT 
TGTGAAAGTA ATCGATCTCA TACTACAATT GCTAAATATG CACAGTACCA GGCCTCCTCC 
TTCCAGGAAT CATTGAGGGA AGAAAATGAG AAAAGAAGTC ACCATAAAGA CCATTCAGAT 
AGTGAATCTA CATCTTCAGA TAATTCTGGG AGGAGGAGGA AAGGACCCTT TAAAACCATA 
AAGTTTGGGA CCAACATTGA CCTGTCTGAT GACAAAAAAT GGAAGTTGCA GCTACATGAG 
CTGACTAAAC TTCCTGCTTT TGTGCGTGTC GTATCAGCAG GAAATCTTCT AAGCCACGTT 
GGTCATACCA TACTGGGCAT GAACACAGTT CAACTGTACA TGAAAGTTCC AGGAAGCAGA 
ACACCAGGTC ATCAAGAAAA TAACAACTTC TGTTCGGTTA ATATAAATAT TGGTCCAGGT 
GACTGTGAAT GGTTTGTTGT CCCTGAAGGT TACTGGGGTG TTCTGAATGA CTTCTGTGAA 
AAAAATAATT TGAATTTCCT AATGGGTTCT TGGTGGCCCA ACCTTGAAGA TCTTTATGAA 
GCAAATGTTC CAGTGTATAG GTTTATTCAG CGACCTGGAG ATTTGGTCTG GATAAATGCA 
GGCACTGTTC ATTGGGTTCA GGCTATTGGC TGGTGCAACA ACATTGCTTG GAATGTTGGT 
CCACTTACAG CCTGCCAGTA TAAATTGGCA GTGGAACGGT ACGAATGGAA CAAATTGCAA 
AGTGTGAAGT CAATAGTACC CATGGTTCAT CTTTCCTGGA ATATGGCACG AAATATCAAG 
GTCTCAGATC CAAAGCTTTT TGAAATGATT AAGTATTGTC TTCTGAGAAC TCTGAAGCAA 
TGTCAGACAT TGAGGGAAGC TCTCATTGCT GCAGGAAAAG AGATTATATG GCATGGGCGG 
ACAAAAGAAG AACCAGCGCA TTACTGTAGC ATTTGTGAGG TGGAGGTTTT TGATCTGCTT 
TTTGTCACAA ATGAGAGCAA TTCTCGAAAG ACCTACATAG TGCATTGCCA AGACTGTGCA 
CGAAAAACAA GTGGAAACCT GGAAAATTTT GTGGTGCTAG AACAGTATAA AATGGAGGAC 
CTGATGCAAG TCTATGACCA ATTTACATTA GCTCCTCCAT TACCATCCTC CTCATCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe34172
Clone ID Related Accession (Same CDS sequence) NM_001197186.1
Accession Version NM_001197186.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 4104bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-04-14
Organism Canis lupus familiaris(dog)
Product lysine-specific demethylase 6A isoform 2
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAEX03026306.1, AY530157.1, AY530158.1 and CF412223.1. On or before Oct 10, 2010 this sequence version replaced XM_857068.1, XM_857099.1, XM_548964.2, XM_857146.1, XM_845565.1, XM_856930.1, XM_856959.1, XM_856992.1, XM_857020.1, XM_857045.1. Transcript Variant: This variant (2) is missing an in-frame coding exon compared to variant 1, resulting in a shorter isoform (2) lacking an internal protein segment compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments, including orthologous human transcript (NM_021140.2). ##Evidence-Data-START## Transcript exon combination :: AY530158.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA103936001, SAMEA103936002 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTATT CGGGTTCGTG 
AGGTTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAC 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAAT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTACA ATGCATTTCA CTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAT 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG CAATCCATGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAG 
TATCATTCTG CAAAGGAAGC TTATGAACAA CTTTTGCAGA CGGAAAACCT TTCTGCACAA 
GTAAAGGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTTCTGGGA 
GATAAAGCTA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTT GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGCGTGCT ATATCAGCAG CAAAATCAGC CTATGGATGC TTTACAGGCT 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACA 
CTCTACGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACCAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG 
TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG 
GAGGCGTGGA GCCTACCAAT CCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA 
GCACAGCAGA ATACTTCTGA TAATTGGAGT AGTGGCCATG CAGTGTCACA TCCTCCAGTA 
CAGCAACAAG CTCATTCGTG GTGTTTGACA CCACAGAAAT TACAGATGAG ACAAACAGGA 
GTTGCACAGG TACGATCTAC TGGAATTCCT AATGGGCCAA CAGCTGACTC ATCACTGCCT 
ACAAACTCAG TCTCTGGCCA GCAGCCACAG CTTGCTCTGA CCAGAGTGCC TAGCGTCTCT 
CAGCCTGGAG TCCGCCCTGC CTGCCCTGGG CAGCCTTTGG CCAACGGACC CTTTTCTGCA 
GGCCATGTTC CCTGTAGCAC ATCAAGAACG CTGGGGAGTA CAGACACTAT ATTGATAGGC 
AATAATCACA TAACAGGAAG TGGAAGTAAT GGAAACGTGC CTTACCTGCA GCGAAACGCA 
CTCACTCTAC CTCATAACCG CACAAACCTG ACCAGCAGCG CAGAGGAGCC GTGGAAAAAC 
CAACTATCTA ACTCCACTCA GGGGCTTCAC AAAGGTCAGA GTTCACATTT GGCAGGTCCT 
AATGGTGAAC GACCTCTCTC TTCCACTGGG CCTTCCCAGC ATCTCCAGGC AGCTGGCTCT 
GGTATTCAGA ATCAGAATGG ACATCCCACC CTGCCTAGCA ATTCAGTAAC ACAGGGGGCT 
GCTCTCAATC ACCTCTCCTC TCACACTGCT ACCTCAGGTG GACAACAAGG CATTACCTTA 
ACCAAAGAGA GCAAGCCTTC AGGAAACACA TCGATGGTGC CTGAAACAAG CAGGCACGCT 
GGAGAGACAC CTAACAGCAC TGCCAGTGTC GAGGGACTTC CTAATCATGT CCATCAGGTG 
ACGGCAGATG CTGTTTGCAG TCCTAGCCAT GGAGATTCTA AGTCACCAGG TTTACTAAGT 
TCAGACAATC CTCAGCTCTC TGCCTTGTTG ATGGGAAAAG CCAATAACAA TGTGGGTACT 
GGAACCTGTG ACAAAGTCAA TAACATCCAC CCAGCTGTTC ATACAAAGAC TGATAATTCT 
GTTGCCTCTT CACCATCTTC AGCCATTTCC ACAGCAACAC CTTCTCCAAA ATCCACTGAG 
CAGACAACCA CAAACAGTGT TACCAGCCTT AATAGCCCTC ACAGTGGGCT ACACACAATT 
AATGGAGAAG GAATGGAGGA ATCTCAGAGC CCCATGAAAA CAGATGTGCT TCTGATTAGC 
CACAAACCTA ATCCTCAGAT CATACCATCA ATGTCTGTAT CCATATACCC CAGCTCAGCA 
GAAGTTCTGA AGGCATGCAG GAATCTAGGT AAAAATGGCT TATCTAACAG TAGCATTTTA 
TTGGATAAAT GTCCGCCTCC AAGACCACCA TCTTCACCAT ACCCTCCCTT GCCAAAGGAC 
AAGTTGAATC CACCTACACC TAGTATTTAT TTGGAAAATA AACGTGATGC TTTCTTTCCT 
CCATTACACC AATTTTGTAC AAATCCAAAC AACCCTGTTA CGGTAATACG TGGCCTTGCT 
GGAGCTCTTA AGTTGGACCT GGGACTTTTC TCTACCAAAA CTTTGGTGGA AGCTAACAAT 
GAACATATGG TGGAAGTGAG GACACAGTTG TTGCAACCAG CAGATGAAAA CTGGGATCCT 
ACTGGAACAA AGAAAATCTG GCATTGTGAA AGTAATCGAT CTCATACTAC AATTGCTAAA 
TATGCACAGT ACCAGGCCTC CTCCTTCCAG GAATCATTGA GGGAAGAAAA TGAGAAAAGA 
AGTCACCATA AAGACCATTC AGATAGTGAA TCTACATCTT CAGATAATTC TGGGAGGAGG 
AGGAAAGGAC CCTTTAAAAC CATAAAGTTT GGGACCAACA TTGACCTGTC TGATGACAAA 
AAATGGAAGT TGCAGCTACA TGAGCTGACT AAACTTCCTG CTTTTGTGCG TGTCGTATCA 
GCAGGAAATC TTCTAAGCCA CGTTGGTCAT ACCATACTGG GCATGAACAC AGTTCAACTG 
TACATGAAAG TTCCAGGAAG CAGAACACCA GGTCATCAAG AAAATAACAA CTTCTGTTCG 
GTTAATATAA ATATTGGTCC AGGTGACTGT GAATGGTTTG TTGTCCCTGA AGGTTACTGG 
GGTGTTCTGA ATGACTTCTG TGAAAAAAAT AATTTGAATT TCCTAATGGG TTCTTGGTGG 
CCCAACCTTG AAGATCTTTA TGAAGCAAAT GTTCCAGTGT ATAGGTTTAT TCAGCGACCT 
GGAGATTTGG TCTGGATAAA TGCAGGCACT GTTCATTGGG TTCAGGCTAT TGGCTGGTGC 
AACAACATTG CTTGGAATGT TGGTCCACTT ACAGCCTGCC AGTATAAATT GGCAGTGGAA 
CGGTACGAAT GGAACAAATT GCAAAGTGTG AAGTCAATAG TACCCATGGT TCATCTTTCC 
TGGAATATGG CACGAAATAT CAAGGTCTCA GATCCAAAGC TTTTTGAAAT GATTAAGTAT 
TGTCTTCTGA GAACTCTGAA GCAATGTCAG ACATTGAGGG AAGCTCTCAT TGCTGCAGGA 
AAAGAGATTA TATGGCATGG GCGGACAAAA GAAGAACCAG CGCATTACTG TAGCATTTGT 
GAGGTGGAGG TTTTTGATCT GCTTTTTGTC ACAAATGAGA GCAATTCTCG AAAGACCTAC 
ATAGTGCATT GCCAAGACTG TGCACGAAAA ACAAGTGGAA ACCTGGAAAA TTTTGTGGTG 
CTAGAACAGT ATAAAATGGA GGACCTGATG CAAGTCTATG ACCAATTTAC ATTAGCTCCT 
CCATTACCAT CCTCCTCATC TTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001184115.1
CDS371..4474
Translation

Target ORF information:

RefSeq Version NM_001197186.1
Organism Canis lupus familiaris(dog)
Definition Canis lupus familiaris lysine demethylase 6A (KDM6A), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001197186.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTATT CGGGTTCGTG 
AGGTTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAC 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAAT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTACA ATGCATTTCA CTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAT 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG CAATCCATGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAG 
TATCATTCTG CAAAGGAAGC TTATGAACAA CTTTTGCAGA CGGAAAACCT TTCTGCACAA 
GTAAAGGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTTCTGGGA 
GATAAAGCTA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTT GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGCGTGCT ATATCAGCAG CAAAATCAGC CTATGGATGC TTTACAGGCT 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACA 
CTCTACGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACCAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG 
TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG 
GAGGCGTGGA GCCTACCAAT CCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA 
GCACAGCAGA ATACTTCTGA TAATTGGAGT AGTGGCCATG CAGTGTCACA TCCTCCAGTA 
CAGCAACAAG CTCATTCGTG GTGTTTGACA CCACAGAAAT TACAGATGAG ACAAACAGGA 
GTTGCACAGG TACGATCTAC TGGAATTCCT AATGGGCCAA CAGCTGACTC ATCACTGCCT 
ACAAACTCAG TCTCTGGCCA GCAGCCACAG CTTGCTCTGA CCAGAGTGCC TAGCGTCTCT 
CAGCCTGGAG TCCGCCCTGC CTGCCCTGGG CAGCCTTTGG CCAACGGACC CTTTTCTGCA 
GGCCATGTTC CCTGTAGCAC ATCAAGAACG CTGGGGAGTA CAGACACTAT ATTGATAGGC 
AATAATCACA TAACAGGAAG TGGAAGTAAT GGAAACGTGC CTTACCTGCA GCGAAACGCA 
CTCACTCTAC CTCATAACCG CACAAACCTG ACCAGCAGCG CAGAGGAGCC GTGGAAAAAC 
CAACTATCTA ACTCCACTCA GGGGCTTCAC AAAGGTCAGA GTTCACATTT GGCAGGTCCT 
AATGGTGAAC GACCTCTCTC TTCCACTGGG CCTTCCCAGC ATCTCCAGGC AGCTGGCTCT 
GGTATTCAGA ATCAGAATGG ACATCCCACC CTGCCTAGCA ATTCAGTAAC ACAGGGGGCT 
GCTCTCAATC ACCTCTCCTC TCACACTGCT ACCTCAGGTG GACAACAAGG CATTACCTTA 
ACCAAAGAGA GCAAGCCTTC AGGAAACACA TCGATGGTGC CTGAAACAAG CAGGCACGCT 
GGAGAGACAC CTAACAGCAC TGCCAGTGTC GAGGGACTTC CTAATCATGT CCATCAGGTG 
ACGGCAGATG CTGTTTGCAG TCCTAGCCAT GGAGATTCTA AGTCACCAGG TTTACTAAGT 
TCAGACAATC CTCAGCTCTC TGCCTTGTTG ATGGGAAAAG CCAATAACAA TGTGGGTACT 
GGAACCTGTG ACAAAGTCAA TAACATCCAC CCAGCTGTTC ATACAAAGAC TGATAATTCT 
GTTGCCTCTT CACCATCTTC AGCCATTTCC ACAGCAACAC CTTCTCCAAA ATCCACTGAG 
CAGACAACCA CAAACAGTGT TACCAGCCTT AATAGCCCTC ACAGTGGGCT ACACACAATT 
AATGGAGAAG GAATGGAGGA ATCTCAGAGC CCCATGAAAA CAGATGTGCT TCTGATTAGC 
CACAAACCTA ATCCTCAGAT CATACCATCA ATGTCTGTAT CCATATACCC CAGCTCAGCA 
GAAGTTCTGA AGGCATGCAG GAATCTAGGT AAAAATGGCT TATCTAACAG TAGCATTTTA 
TTGGATAAAT GTCCGCCTCC AAGACCACCA TCTTCACCAT ACCCTCCCTT GCCAAAGGAC 
AAGTTGAATC CACCTACACC TAGTATTTAT TTGGAAAATA AACGTGATGC TTTCTTTCCT 
CCATTACACC AATTTTGTAC AAATCCAAAC AACCCTGTTA CGGTAATACG TGGCCTTGCT 
GGAGCTCTTA AGTTGGACCT GGGACTTTTC TCTACCAAAA CTTTGGTGGA AGCTAACAAT 
GAACATATGG TGGAAGTGAG GACACAGTTG TTGCAACCAG CAGATGAAAA CTGGGATCCT 
ACTGGAACAA AGAAAATCTG GCATTGTGAA AGTAATCGAT CTCATACTAC AATTGCTAAA 
TATGCACAGT ACCAGGCCTC CTCCTTCCAG GAATCATTGA GGGAAGAAAA TGAGAAAAGA 
AGTCACCATA AAGACCATTC AGATAGTGAA TCTACATCTT CAGATAATTC TGGGAGGAGG 
AGGAAAGGAC CCTTTAAAAC CATAAAGTTT GGGACCAACA TTGACCTGTC TGATGACAAA 
AAATGGAAGT TGCAGCTACA TGAGCTGACT AAACTTCCTG CTTTTGTGCG TGTCGTATCA 
GCAGGAAATC TTCTAAGCCA CGTTGGTCAT ACCATACTGG GCATGAACAC AGTTCAACTG 
TACATGAAAG TTCCAGGAAG CAGAACACCA GGTCATCAAG AAAATAACAA CTTCTGTTCG 
GTTAATATAA ATATTGGTCC AGGTGACTGT GAATGGTTTG TTGTCCCTGA AGGTTACTGG 
GGTGTTCTGA ATGACTTCTG TGAAAAAAAT AATTTGAATT TCCTAATGGG TTCTTGGTGG 
CCCAACCTTG AAGATCTTTA TGAAGCAAAT GTTCCAGTGT ATAGGTTTAT TCAGCGACCT 
GGAGATTTGG TCTGGATAAA TGCAGGCACT GTTCATTGGG TTCAGGCTAT TGGCTGGTGC 
AACAACATTG CTTGGAATGT TGGTCCACTT ACAGCCTGCC AGTATAAATT GGCAGTGGAA 
CGGTACGAAT GGAACAAATT GCAAAGTGTG AAGTCAATAG TACCCATGGT TCATCTTTCC 
TGGAATATGG CACGAAATAT CAAGGTCTCA GATCCAAAGC TTTTTGAAAT GATTAAGTAT 
TGTCTTCTGA GAACTCTGAA GCAATGTCAG ACATTGAGGG AAGCTCTCAT TGCTGCAGGA 
AAAGAGATTA TATGGCATGG GCGGACAAAA GAAGAACCAG CGCATTACTG TAGCATTTGT 
GAGGTGGAGG TTTTTGATCT GCTTTTTGTC ACAAATGAGA GCAATTCTCG AAAGACCTAC 
ATAGTGCATT GCCAAGACTG TGCACGAAAA ACAAGTGGAA ACCTGGAAAA TTTTGTGGTG 
CTAGAACAGT ATAAAATGGA GGACCTGATG CAAGTCTATG ACCAATTTAC ATTAGCTCCT 
CCATTACCAT CCTCCTCATC TTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe31949
Clone ID Related Accession (Same CDS sequence) XM_005641004.2 , XM_005641004.3
Accession Version XM_005641004.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 4362bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2015-09-16
Organism Canis lupus familiaris(Dog)
Product lysine-specific demethylase 6A isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005641004.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTATT CGGGTTCGTG 
AGGTTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAC 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAAT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTACA ATGCATTTCA CTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAT 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG CAATCCATGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAG 
TATCATTCTG CAAAGGAAGC TTATGAACAA CTTTTGCAGA CGGAAAACCT TTCTGCACAA 
GTAAAGGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTTCTGGGA 
GATAAAGCTA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTT GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGCGTGCT ATATCAGCAG CAAAATCAGC CTATGGATGC TTTACAGGCT 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACA 
CTCTACGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACCAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG 
TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG 
GAGGCGTGGA GCCTACCAAT CCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA 
GCACAGCAGG CATGTAAACC TCATCATCCA AATACTGAAC CTGTATTAGG CCTCAGTCAA 
ACACCAATTT CACAGCAGTC CTTGCCACTA CACATGATTC CTTCTAGCCA AGTAGATGAC 
CTGTCCAGTC CTGCCAAGAG GAAAAGAACA TCTAGTCCAA CAAAGAATAC TTCTGATAAT 
TGGAGTAGTG GCCATGCAGT GTCACATCCT CCAGTACAGC AACAAGCTCA TTCGTGGTGT 
TTGACACCAC AGAAATTACA GCACTTGGAA CAGCTCCGTG CAAATAGAAA TAACTTAAAT 
CCAGCACAGA AACTGATGCT GGAACAGCTG GAAAGTCAGT TTGTCTTAAT GCAACAACAC 
CAAATGAGAC AAACAGGAGT TGCACAGGTA CGATCTACTG GAATTCCTAA TGGGCCAACA 
GCTGACTCAT CACTGCCTAC AAACTCAGTC TCTGGCCAGC AGCCACAGCT TGCTCTGACC 
AGAGTGCCTA GCGTCTCTCA GCCTGGAGTC CGCCCTGCCT GCCCTGGGCA GCCTTTGGCC 
AACGGACCCT TTTCTGCAGG CCATGTTCCC TGTAGCACAT CAAGAACGCT GGGGAGTACA 
GACACTATAT TGATAGGCAA TAATCACATA ACAGGAAGTG GAAGTAATGG AAACGTGCCT 
TACCTGCAGC GAAACGCACT CACTCTACCT CATAACCGCA CAAACCTGAC CAGCAGCGCA 
GAGGAGCCGT GGAAAAACCA ACTATCTAAC TCCACTCAGG GGCTTCACAA AGGTCAGAGT 
TCACATTTGG CAGGTCCTAA TGGTGAACGA CCTCTCTCTT CCACTGGGCC TTCCCAGCAT 
CTCCAGGCAG CTGGCTCTGG TATTCAGAAT CAGAATGGAC ATCCCACCCT GCCTAGCAAT 
TCAGTAACAC AGGGGGCTGC TCTCAATCAC CTCTCCTCTC ACACTGCTAC CTCAGGTGGA 
CAACAAGGCA TTACCTTAAC CAAAGAGAGC AAGCCTTCAG GAAACACATC GATGGTGCCT 
GAAACAAGCA GGCACGCTGG AGAGACACCT AACAGCACTG CCAGTGTCGA GGGACTTCCT 
AATCATGTCC ATCAGGTGAC GGCAGATGCT GTTTGCAGTC CTAGCCATGG AGATTCTAAG 
TCACCAGGTT TACTAAGTTC AGACAATCCT CAGCTCTCTG CCTTGTTGAT GGGAAAAGCC 
AATAACAATG TGGGTACTGG AACCTGTGAC AAAGTCAATA ACATCCACCC AGCTGTTCAT 
ACAAAGACTG ATAATTCTGT TGCCTCTTCA CCATCTTCAG CCATTTCCAC AGCAACACCT 
TCTCCAAAAT CCACTGAGCA GACAACCACA AACAGTGTTA CCAGCCTTAA TAGCCCTCAC 
AGTGGGCTAC ACACAATTAA TGGAGAAGGA ATGGAGGAAT CTCAGAGCCC CATGAAAACA 
GATGTGCTTC TGATTAGCCA CAAACCTAAT CCTCAGATCA TACCATCAAT GTCTGTATCC 
ATATACCCCA GCTCAGCAGA AGTTCTGAAG GCATGCAGGA ATCTAGGTAA AAATGGCTTA 
TCTAACAGTA GCATTTTATT GGATAAATGT CCGCCTCCAA GACCACCATC TTCACCATAC 
CCTCCCTTGC CAAAGGACAA GTTGAATCCA CCTACACCTA GTATTTATTT GGAAAATAAA 
CGTGATGCTT TCTTTCCTCC ATTACACCAA TTTTGTACAA ATCCAAACAA CCCTGTTACG 
GTAATACGTG GCCTTGCTGG AGCTCTTAAG TTGGACCTGG GACTTTTCTC TACCAAAACT 
TTGGTGGAAG CTAACAATGA ACATATGGTG GAAGTGAGGA CACAGTTGTT GCAACCAGCA 
GATGAAAACT GGGATCCTAC TGGAACAAAG AAAATCTGGC ATTGTGAAAG TAATCGATCT 
CATACTACAA TTGCTAAATA TGCACAGTAC CAGGCCTCCT CCTTCCAGGA ATCATTGAGG 
GAAGAAAATG AGAAAAGAAG TCACCATAAA GACCATTCAG ATAGTGAATC TACATCTTCA 
GATAATTCTG GGAGGAGGAG GAAAGGACCC TTTAAAACCA TAAAGTTTGG GACCAACATT 
GACCTGTCTG ATGACAAAAA ATGGAAGTTG CAGCTACATG AGCTGACTAA ACTTCCTGCT 
TTTGTGCGTG TCGTATCAGC AGGAAATCTT CTAAGCCACG TTGGTCATAC CATACTGGGC 
ATGAACACAG TTCAACTGTA CATGAAAGTT CCAGGAAGCA GAACACCAGG TCATCAAGAA 
AATAACAACT TCTGTTCGGT TAATATAAAT ATTGGTCCAG GTGACTGTGA ATGGTTTGTT 
GTCCCTGAAG GTTACTGGGG TGTTCTGAAT GACTTCTGTG AAAAAAATAA TTTGAATTTC 
CTAATGGGTT CTTGGTGGCC CAACCTTGAA GATCTTTATG AAGCAAATGT TCCAGTGTAT 
AGGTTTATTC AGCGACCTGG AGATTTGGTC TGGATAAATG CAGGCACTGT TCATTGGGTT 
CAGGCTATTG GCTGGTGCAA CAACATTGCT TGGAATGTTG GTCCACTTAC AGCCTGCCAG 
TATAAATTGG CAGTGGAACG GTACGAATGG AACAAATTGC AAAGTGTGAA GTCAATAGTA 
CCCATGGTTC ATCTTTCCTG GAATATGGCA CGAAATATCA AGGTCTCAGA TCCAAAGCTT 
TTTGAAATGA TTAAGTATTG TCTTCTGAGA ACTCTGAAGC AATGTCAGAC ATTGAGGGAA 
GCTCTCATTG CTGCAGGAAA AGAGATTATA TGGCATGGGC GGACAAAAGA AGAACCAGCG 
CATTACTGTA GCATTTGTGA GGTGGAGGTT TTTGATCTGC TTTTTGTCAC AAATGAGAGC 
AATTCTCGAA AGACCTACAT AGTGCATTGC CAAGACTGTG CACGAAAAAC AAGTGGAAAC 
CTGGAAAATT TTGTGGTGCT AGAACAGTAT AAAATGGAGG ACCTGATGCA AGTCTATGAC 
CAATTTACAT TAGCTCCTCC ATTACCATCC TCCTCATCTT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005641061.1
CDS381..4742
Translation

Target ORF information:

RefSeq Version XM_005641004.2
Organism Canis lupus familiaris(Dog)
Definition Canis lupus familiaris lysine (K)-specific demethylase 6A (KDM6A), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005641004.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTATT CGGGTTCGTG 
AGGTTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAC 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAAT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTACA ATGCATTTCA CTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAT 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG CAATCCATGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAG 
TATCATTCTG CAAAGGAAGC TTATGAACAA CTTTTGCAGA CGGAAAACCT TTCTGCACAA 
GTAAAGGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTTCTGGGA 
GATAAAGCTA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTT GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGCGTGCT ATATCAGCAG CAAAATCAGC CTATGGATGC TTTACAGGCT 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACA 
CTCTACGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACCAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG 
TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG 
GAGGCGTGGA GCCTACCAAT CCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA 
GCACAGCAGG CATGTAAACC TCATCATCCA AATACTGAAC CTGTATTAGG CCTCAGTCAA 
ACACCAATTT CACAGCAGTC CTTGCCACTA CACATGATTC CTTCTAGCCA AGTAGATGAC 
CTGTCCAGTC CTGCCAAGAG GAAAAGAACA TCTAGTCCAA CAAAGAATAC TTCTGATAAT 
TGGAGTAGTG GCCATGCAGT GTCACATCCT CCAGTACAGC AACAAGCTCA TTCGTGGTGT 
TTGACACCAC AGAAATTACA GCACTTGGAA CAGCTCCGTG CAAATAGAAA TAACTTAAAT 
CCAGCACAGA AACTGATGCT GGAACAGCTG GAAAGTCAGT TTGTCTTAAT GCAACAACAC 
CAAATGAGAC AAACAGGAGT TGCACAGGTA CGATCTACTG GAATTCCTAA TGGGCCAACA 
GCTGACTCAT CACTGCCTAC AAACTCAGTC TCTGGCCAGC AGCCACAGCT TGCTCTGACC 
AGAGTGCCTA GCGTCTCTCA GCCTGGAGTC CGCCCTGCCT GCCCTGGGCA GCCTTTGGCC 
AACGGACCCT TTTCTGCAGG CCATGTTCCC TGTAGCACAT CAAGAACGCT GGGGAGTACA 
GACACTATAT TGATAGGCAA TAATCACATA ACAGGAAGTG GAAGTAATGG AAACGTGCCT 
TACCTGCAGC GAAACGCACT CACTCTACCT CATAACCGCA CAAACCTGAC CAGCAGCGCA 
GAGGAGCCGT GGAAAAACCA ACTATCTAAC TCCACTCAGG GGCTTCACAA AGGTCAGAGT 
TCACATTTGG CAGGTCCTAA TGGTGAACGA CCTCTCTCTT CCACTGGGCC TTCCCAGCAT 
CTCCAGGCAG CTGGCTCTGG TATTCAGAAT CAGAATGGAC ATCCCACCCT GCCTAGCAAT 
TCAGTAACAC AGGGGGCTGC TCTCAATCAC CTCTCCTCTC ACACTGCTAC CTCAGGTGGA 
CAACAAGGCA TTACCTTAAC CAAAGAGAGC AAGCCTTCAG GAAACACATC GATGGTGCCT 
GAAACAAGCA GGCACGCTGG AGAGACACCT AACAGCACTG CCAGTGTCGA GGGACTTCCT 
AATCATGTCC ATCAGGTGAC GGCAGATGCT GTTTGCAGTC CTAGCCATGG AGATTCTAAG 
TCACCAGGTT TACTAAGTTC AGACAATCCT CAGCTCTCTG CCTTGTTGAT GGGAAAAGCC 
AATAACAATG TGGGTACTGG AACCTGTGAC AAAGTCAATA ACATCCACCC AGCTGTTCAT 
ACAAAGACTG ATAATTCTGT TGCCTCTTCA CCATCTTCAG CCATTTCCAC AGCAACACCT 
TCTCCAAAAT CCACTGAGCA GACAACCACA AACAGTGTTA CCAGCCTTAA TAGCCCTCAC 
AGTGGGCTAC ACACAATTAA TGGAGAAGGA ATGGAGGAAT CTCAGAGCCC CATGAAAACA 
GATGTGCTTC TGATTAGCCA CAAACCTAAT CCTCAGATCA TACCATCAAT GTCTGTATCC 
ATATACCCCA GCTCAGCAGA AGTTCTGAAG GCATGCAGGA ATCTAGGTAA AAATGGCTTA 
TCTAACAGTA GCATTTTATT GGATAAATGT CCGCCTCCAA GACCACCATC TTCACCATAC 
CCTCCCTTGC CAAAGGACAA GTTGAATCCA CCTACACCTA GTATTTATTT GGAAAATAAA 
CGTGATGCTT TCTTTCCTCC ATTACACCAA TTTTGTACAA ATCCAAACAA CCCTGTTACG 
GTAATACGTG GCCTTGCTGG AGCTCTTAAG TTGGACCTGG GACTTTTCTC TACCAAAACT 
TTGGTGGAAG CTAACAATGA ACATATGGTG GAAGTGAGGA CACAGTTGTT GCAACCAGCA 
GATGAAAACT GGGATCCTAC TGGAACAAAG AAAATCTGGC ATTGTGAAAG TAATCGATCT 
CATACTACAA TTGCTAAATA TGCACAGTAC CAGGCCTCCT CCTTCCAGGA ATCATTGAGG 
GAAGAAAATG AGAAAAGAAG TCACCATAAA GACCATTCAG ATAGTGAATC TACATCTTCA 
GATAATTCTG GGAGGAGGAG GAAAGGACCC TTTAAAACCA TAAAGTTTGG GACCAACATT 
GACCTGTCTG ATGACAAAAA ATGGAAGTTG CAGCTACATG AGCTGACTAA ACTTCCTGCT 
TTTGTGCGTG TCGTATCAGC AGGAAATCTT CTAAGCCACG TTGGTCATAC CATACTGGGC 
ATGAACACAG TTCAACTGTA CATGAAAGTT CCAGGAAGCA GAACACCAGG TCATCAAGAA 
AATAACAACT TCTGTTCGGT TAATATAAAT ATTGGTCCAG GTGACTGTGA ATGGTTTGTT 
GTCCCTGAAG GTTACTGGGG TGTTCTGAAT GACTTCTGTG AAAAAAATAA TTTGAATTTC 
CTAATGGGTT CTTGGTGGCC CAACCTTGAA GATCTTTATG AAGCAAATGT TCCAGTGTAT 
AGGTTTATTC AGCGACCTGG AGATTTGGTC TGGATAAATG CAGGCACTGT TCATTGGGTT 
CAGGCTATTG GCTGGTGCAA CAACATTGCT TGGAATGTTG GTCCACTTAC AGCCTGCCAG 
TATAAATTGG CAGTGGAACG GTACGAATGG AACAAATTGC AAAGTGTGAA GTCAATAGTA 
CCCATGGTTC ATCTTTCCTG GAATATGGCA CGAAATATCA AGGTCTCAGA TCCAAAGCTT 
TTTGAAATGA TTAAGTATTG TCTTCTGAGA ACTCTGAAGC AATGTCAGAC ATTGAGGGAA 
GCTCTCATTG CTGCAGGAAA AGAGATTATA TGGCATGGGC GGACAAAAGA AGAACCAGCG 
CATTACTGTA GCATTTGTGA GGTGGAGGTT TTTGATCTGC TTTTTGTCAC AAATGAGAGC 
AATTCTCGAA AGACCTACAT AGTGCATTGC CAAGACTGTG CACGAAAAAC AAGTGGAAAC 
CTGGAAAATT TTGTGGTGCT AGAACAGTAT AAAATGGAGG ACCTGATGCA AGTCTATGAC 
CAATTTACAT TAGCTCCTCC ATTACCATCC TCCTCATCTT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe31949
Clone ID Related Accession (Same CDS sequence) XM_005641004.2 , XM_005641004.3
Accession Version XM_005641004.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 4362bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-09-04
Organism Canis lupus familiaris(dog)
Product lysine-specific demethylase 6A isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005641004.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTATT CGGGTTCGTG 
AGGTTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAC 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAAT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTACA ATGCATTTCA CTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAT 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG CAATCCATGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAG 
TATCATTCTG CAAAGGAAGC TTATGAACAA CTTTTGCAGA CGGAAAACCT TTCTGCACAA 
GTAAAGGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTTCTGGGA 
GATAAAGCTA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTT GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGCGTGCT ATATCAGCAG CAAAATCAGC CTATGGATGC TTTACAGGCT 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACA 
CTCTACGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACCAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG 
TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG 
GAGGCGTGGA GCCTACCAAT CCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA 
GCACAGCAGG CATGTAAACC TCATCATCCA AATACTGAAC CTGTATTAGG CCTCAGTCAA 
ACACCAATTT CACAGCAGTC CTTGCCACTA CACATGATTC CTTCTAGCCA AGTAGATGAC 
CTGTCCAGTC CTGCCAAGAG GAAAAGAACA TCTAGTCCAA CAAAGAATAC TTCTGATAAT 
TGGAGTAGTG GCCATGCAGT GTCACATCCT CCAGTACAGC AACAAGCTCA TTCGTGGTGT 
TTGACACCAC AGAAATTACA GCACTTGGAA CAGCTCCGTG CAAATAGAAA TAACTTAAAT 
CCAGCACAGA AACTGATGCT GGAACAGCTG GAAAGTCAGT TTGTCTTAAT GCAACAACAC 
CAAATGAGAC AAACAGGAGT TGCACAGGTA CGATCTACTG GAATTCCTAA TGGGCCAACA 
GCTGACTCAT CACTGCCTAC AAACTCAGTC TCTGGCCAGC AGCCACAGCT TGCTCTGACC 
AGAGTGCCTA GCGTCTCTCA GCCTGGAGTC CGCCCTGCCT GCCCTGGGCA GCCTTTGGCC 
AACGGACCCT TTTCTGCAGG CCATGTTCCC TGTAGCACAT CAAGAACGCT GGGGAGTACA 
GACACTATAT TGATAGGCAA TAATCACATA ACAGGAAGTG GAAGTAATGG AAACGTGCCT 
TACCTGCAGC GAAACGCACT CACTCTACCT CATAACCGCA CAAACCTGAC CAGCAGCGCA 
GAGGAGCCGT GGAAAAACCA ACTATCTAAC TCCACTCAGG GGCTTCACAA AGGTCAGAGT 
TCACATTTGG CAGGTCCTAA TGGTGAACGA CCTCTCTCTT CCACTGGGCC TTCCCAGCAT 
CTCCAGGCAG CTGGCTCTGG TATTCAGAAT CAGAATGGAC ATCCCACCCT GCCTAGCAAT 
TCAGTAACAC AGGGGGCTGC TCTCAATCAC CTCTCCTCTC ACACTGCTAC CTCAGGTGGA 
CAACAAGGCA TTACCTTAAC CAAAGAGAGC AAGCCTTCAG GAAACACATC GATGGTGCCT 
GAAACAAGCA GGCACGCTGG AGAGACACCT AACAGCACTG CCAGTGTCGA GGGACTTCCT 
AATCATGTCC ATCAGGTGAC GGCAGATGCT GTTTGCAGTC CTAGCCATGG AGATTCTAAG 
TCACCAGGTT TACTAAGTTC AGACAATCCT CAGCTCTCTG CCTTGTTGAT GGGAAAAGCC 
AATAACAATG TGGGTACTGG AACCTGTGAC AAAGTCAATA ACATCCACCC AGCTGTTCAT 
ACAAAGACTG ATAATTCTGT TGCCTCTTCA CCATCTTCAG CCATTTCCAC AGCAACACCT 
TCTCCAAAAT CCACTGAGCA GACAACCACA AACAGTGTTA CCAGCCTTAA TAGCCCTCAC 
AGTGGGCTAC ACACAATTAA TGGAGAAGGA ATGGAGGAAT CTCAGAGCCC CATGAAAACA 
GATGTGCTTC TGATTAGCCA CAAACCTAAT CCTCAGATCA TACCATCAAT GTCTGTATCC 
ATATACCCCA GCTCAGCAGA AGTTCTGAAG GCATGCAGGA ATCTAGGTAA AAATGGCTTA 
TCTAACAGTA GCATTTTATT GGATAAATGT CCGCCTCCAA GACCACCATC TTCACCATAC 
CCTCCCTTGC CAAAGGACAA GTTGAATCCA CCTACACCTA GTATTTATTT GGAAAATAAA 
CGTGATGCTT TCTTTCCTCC ATTACACCAA TTTTGTACAA ATCCAAACAA CCCTGTTACG 
GTAATACGTG GCCTTGCTGG AGCTCTTAAG TTGGACCTGG GACTTTTCTC TACCAAAACT 
TTGGTGGAAG CTAACAATGA ACATATGGTG GAAGTGAGGA CACAGTTGTT GCAACCAGCA 
GATGAAAACT GGGATCCTAC TGGAACAAAG AAAATCTGGC ATTGTGAAAG TAATCGATCT 
CATACTACAA TTGCTAAATA TGCACAGTAC CAGGCCTCCT CCTTCCAGGA ATCATTGAGG 
GAAGAAAATG AGAAAAGAAG TCACCATAAA GACCATTCAG ATAGTGAATC TACATCTTCA 
GATAATTCTG GGAGGAGGAG GAAAGGACCC TTTAAAACCA TAAAGTTTGG GACCAACATT 
GACCTGTCTG ATGACAAAAA ATGGAAGTTG CAGCTACATG AGCTGACTAA ACTTCCTGCT 
TTTGTGCGTG TCGTATCAGC AGGAAATCTT CTAAGCCACG TTGGTCATAC CATACTGGGC 
ATGAACACAG TTCAACTGTA CATGAAAGTT CCAGGAAGCA GAACACCAGG TCATCAAGAA 
AATAACAACT TCTGTTCGGT TAATATAAAT ATTGGTCCAG GTGACTGTGA ATGGTTTGTT 
GTCCCTGAAG GTTACTGGGG TGTTCTGAAT GACTTCTGTG AAAAAAATAA TTTGAATTTC 
CTAATGGGTT CTTGGTGGCC CAACCTTGAA GATCTTTATG AAGCAAATGT TCCAGTGTAT 
AGGTTTATTC AGCGACCTGG AGATTTGGTC TGGATAAATG CAGGCACTGT TCATTGGGTT 
CAGGCTATTG GCTGGTGCAA CAACATTGCT TGGAATGTTG GTCCACTTAC AGCCTGCCAG 
TATAAATTGG CAGTGGAACG GTACGAATGG AACAAATTGC AAAGTGTGAA GTCAATAGTA 
CCCATGGTTC ATCTTTCCTG GAATATGGCA CGAAATATCA AGGTCTCAGA TCCAAAGCTT 
TTTGAAATGA TTAAGTATTG TCTTCTGAGA ACTCTGAAGC AATGTCAGAC ATTGAGGGAA 
GCTCTCATTG CTGCAGGAAA AGAGATTATA TGGCATGGGC GGACAAAAGA AGAACCAGCG 
CATTACTGTA GCATTTGTGA GGTGGAGGTT TTTGATCTGC TTTTTGTCAC AAATGAGAGC 
AATTCTCGAA AGACCTACAT AGTGCATTGC CAAGACTGTG CACGAAAAAC AAGTGGAAAC 
CTGGAAAATT TTGTGGTGCT AGAACAGTAT AAAATGGAGG ACCTGATGCA AGTCTATGAC 
CAATTTACAT TAGCTCCTCC ATTACCATCC TCCTCATCTT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005641061.1
CDS200..4561
Translation

Target ORF information:

RefSeq Version XM_005641004.3
Organism Canis lupus familiaris(dog)
Definition Canis lupus familiaris lysine demethylase 6A (KDM6A), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005641004.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTATT CGGGTTCGTG 
AGGTTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAC 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAAT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTACA ATGCATTTCA CTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAT 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG CAATCCATGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAG 
TATCATTCTG CAAAGGAAGC TTATGAACAA CTTTTGCAGA CGGAAAACCT TTCTGCACAA 
GTAAAGGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTTCTGGGA 
GATAAAGCTA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTT GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGCGTGCT ATATCAGCAG CAAAATCAGC CTATGGATGC TTTACAGGCT 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACA 
CTCTACGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACCAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG 
TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG 
GAGGCGTGGA GCCTACCAAT CCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA 
GCACAGCAGG CATGTAAACC TCATCATCCA AATACTGAAC CTGTATTAGG CCTCAGTCAA 
ACACCAATTT CACAGCAGTC CTTGCCACTA CACATGATTC CTTCTAGCCA AGTAGATGAC 
CTGTCCAGTC CTGCCAAGAG GAAAAGAACA TCTAGTCCAA CAAAGAATAC TTCTGATAAT 
TGGAGTAGTG GCCATGCAGT GTCACATCCT CCAGTACAGC AACAAGCTCA TTCGTGGTGT 
TTGACACCAC AGAAATTACA GCACTTGGAA CAGCTCCGTG CAAATAGAAA TAACTTAAAT 
CCAGCACAGA AACTGATGCT GGAACAGCTG GAAAGTCAGT TTGTCTTAAT GCAACAACAC 
CAAATGAGAC AAACAGGAGT TGCACAGGTA CGATCTACTG GAATTCCTAA TGGGCCAACA 
GCTGACTCAT CACTGCCTAC AAACTCAGTC TCTGGCCAGC AGCCACAGCT TGCTCTGACC 
AGAGTGCCTA GCGTCTCTCA GCCTGGAGTC CGCCCTGCCT GCCCTGGGCA GCCTTTGGCC 
AACGGACCCT TTTCTGCAGG CCATGTTCCC TGTAGCACAT CAAGAACGCT GGGGAGTACA 
GACACTATAT TGATAGGCAA TAATCACATA ACAGGAAGTG GAAGTAATGG AAACGTGCCT 
TACCTGCAGC GAAACGCACT CACTCTACCT CATAACCGCA CAAACCTGAC CAGCAGCGCA 
GAGGAGCCGT GGAAAAACCA ACTATCTAAC TCCACTCAGG GGCTTCACAA AGGTCAGAGT 
TCACATTTGG CAGGTCCTAA TGGTGAACGA CCTCTCTCTT CCACTGGGCC TTCCCAGCAT 
CTCCAGGCAG CTGGCTCTGG TATTCAGAAT CAGAATGGAC ATCCCACCCT GCCTAGCAAT 
TCAGTAACAC AGGGGGCTGC TCTCAATCAC CTCTCCTCTC ACACTGCTAC CTCAGGTGGA 
CAACAAGGCA TTACCTTAAC CAAAGAGAGC AAGCCTTCAG GAAACACATC GATGGTGCCT 
GAAACAAGCA GGCACGCTGG AGAGACACCT AACAGCACTG CCAGTGTCGA GGGACTTCCT 
AATCATGTCC ATCAGGTGAC GGCAGATGCT GTTTGCAGTC CTAGCCATGG AGATTCTAAG 
TCACCAGGTT TACTAAGTTC AGACAATCCT CAGCTCTCTG CCTTGTTGAT GGGAAAAGCC 
AATAACAATG TGGGTACTGG AACCTGTGAC AAAGTCAATA ACATCCACCC AGCTGTTCAT 
ACAAAGACTG ATAATTCTGT TGCCTCTTCA CCATCTTCAG CCATTTCCAC AGCAACACCT 
TCTCCAAAAT CCACTGAGCA GACAACCACA AACAGTGTTA CCAGCCTTAA TAGCCCTCAC 
AGTGGGCTAC ACACAATTAA TGGAGAAGGA ATGGAGGAAT CTCAGAGCCC CATGAAAACA 
GATGTGCTTC TGATTAGCCA CAAACCTAAT CCTCAGATCA TACCATCAAT GTCTGTATCC 
ATATACCCCA GCTCAGCAGA AGTTCTGAAG GCATGCAGGA ATCTAGGTAA AAATGGCTTA 
TCTAACAGTA GCATTTTATT GGATAAATGT CCGCCTCCAA GACCACCATC TTCACCATAC 
CCTCCCTTGC CAAAGGACAA GTTGAATCCA CCTACACCTA GTATTTATTT GGAAAATAAA 
CGTGATGCTT TCTTTCCTCC ATTACACCAA TTTTGTACAA ATCCAAACAA CCCTGTTACG 
GTAATACGTG GCCTTGCTGG AGCTCTTAAG TTGGACCTGG GACTTTTCTC TACCAAAACT 
TTGGTGGAAG CTAACAATGA ACATATGGTG GAAGTGAGGA CACAGTTGTT GCAACCAGCA 
GATGAAAACT GGGATCCTAC TGGAACAAAG AAAATCTGGC ATTGTGAAAG TAATCGATCT 
CATACTACAA TTGCTAAATA TGCACAGTAC CAGGCCTCCT CCTTCCAGGA ATCATTGAGG 
GAAGAAAATG AGAAAAGAAG TCACCATAAA GACCATTCAG ATAGTGAATC TACATCTTCA 
GATAATTCTG GGAGGAGGAG GAAAGGACCC TTTAAAACCA TAAAGTTTGG GACCAACATT 
GACCTGTCTG ATGACAAAAA ATGGAAGTTG CAGCTACATG AGCTGACTAA ACTTCCTGCT 
TTTGTGCGTG TCGTATCAGC AGGAAATCTT CTAAGCCACG TTGGTCATAC CATACTGGGC 
ATGAACACAG TTCAACTGTA CATGAAAGTT CCAGGAAGCA GAACACCAGG TCATCAAGAA 
AATAACAACT TCTGTTCGGT TAATATAAAT ATTGGTCCAG GTGACTGTGA ATGGTTTGTT 
GTCCCTGAAG GTTACTGGGG TGTTCTGAAT GACTTCTGTG AAAAAAATAA TTTGAATTTC 
CTAATGGGTT CTTGGTGGCC CAACCTTGAA GATCTTTATG AAGCAAATGT TCCAGTGTAT 
AGGTTTATTC AGCGACCTGG AGATTTGGTC TGGATAAATG CAGGCACTGT TCATTGGGTT 
CAGGCTATTG GCTGGTGCAA CAACATTGCT TGGAATGTTG GTCCACTTAC AGCCTGCCAG 
TATAAATTGG CAGTGGAACG GTACGAATGG AACAAATTGC AAAGTGTGAA GTCAATAGTA 
CCCATGGTTC ATCTTTCCTG GAATATGGCA CGAAATATCA AGGTCTCAGA TCCAAAGCTT 
TTTGAAATGA TTAAGTATTG TCTTCTGAGA ACTCTGAAGC AATGTCAGAC ATTGAGGGAA 
GCTCTCATTG CTGCAGGAAA AGAGATTATA TGGCATGGGC GGACAAAAGA AGAACCAGCG 
CATTACTGTA GCATTTGTGA GGTGGAGGTT TTTGATCTGC TTTTTGTCAC AAATGAGAGC 
AATTCTCGAA AGACCTACAT AGTGCATTGC CAAGACTGTG CACGAAAAAC AAGTGGAAAC 
CTGGAAAATT TTGTGGTGCT AGAACAGTAT AAAATGGAGG ACCTGATGCA AGTCTATGAC 
CAATTTACAT TAGCTCCTCC ATTACCATCC TCCTCATCTT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe31950
Clone ID Related Accession (Same CDS sequence) XM_005641005.3 , XM_005641005.2
Accession Version XM_005641005.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 4227bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-09-04
Organism Canis lupus familiaris(dog)
Product lysine-specific demethylase 6A isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005641005.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTATT CGGGTTCGTG 
AGGTTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAC 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAAT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTACA ATGCATTTCA CTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAT 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG CAATCCATGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAG 
TATCATTCTG CAAAGGAAGC TTATGAACAA CTTTTGCAGA CGGAAAACCT TTCTGCACAA 
GTAAAGGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTTCTGGGA 
GATAAAGCTA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTT GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGCGTGCT ATATCAGCAG CAAAATCAGC CTATGGATGC TTTACAGGCT 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACA 
CTCTACGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACCAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCATGT 
AAACCTCATC ATCCAAATAC TGAACCTGTA TTAGGCCTCA GTCAAACACC AATTTCACAG 
CAGTCCTTGC CACTACACAT GATTCCTTCT AGCCAAGTAG ATGACCTGTC CAGTCCTGCC 
AAGAGGAAAA GAACATCTAG TCCAACAAAG AATACTTCTG ATAATTGGAG TAGTGGCCAT 
GCAGTGTCAC ATCCTCCAGT ACAGCAACAA GCTCATTCGT GGTGTTTGAC ACCACAGAAA 
TTACAGCACT TGGAACAGCT CCGTGCAAAT AGAAATAACT TAAATCCAGC ACAGAAACTG 
ATGCTGGAAC AGCTGGAAAG TCAGTTTGTC TTAATGCAAC AACACCAAAT GAGACAAACA 
GGAGTTGCAC AGGTACGATC TACTGGAATT CCTAATGGGC CAACAGCTGA CTCATCACTG 
CCTACAAACT CAGTCTCTGG CCAGCAGCCA CAGCTTGCTC TGACCAGAGT GCCTAGCGTC 
TCTCAGCCTG GAGTCCGCCC TGCCTGCCCT GGGCAGCCTT TGGCCAACGG ACCCTTTTCT 
GCAGGCCATG TTCCCTGTAG CACATCAAGA ACGCTGGGGA GTACAGACAC TATATTGATA 
GGCAATAATC ACATAACAGG AAGTGGAAGT AATGGAAACG TGCCTTACCT GCAGCGAAAC 
GCACTCACTC TACCTCATAA CCGCACAAAC CTGACCAGCA GCGCAGAGGA GCCGTGGAAA 
AACCAACTAT CTAACTCCAC TCAGGGGCTT CACAAAGGTC AGAGTTCACA TTTGGCAGGT 
CCTAATGGTG AACGACCTCT CTCTTCCACT GGGCCTTCCC AGCATCTCCA GGCAGCTGGC 
TCTGGTATTC AGAATCAGAA TGGACATCCC ACCCTGCCTA GCAATTCAGT AACACAGGGG 
GCTGCTCTCA ATCACCTCTC CTCTCACACT GCTACCTCAG GTGGACAACA AGGCATTACC 
TTAACCAAAG AGAGCAAGCC TTCAGGAAAC ACATCGATGG TGCCTGAAAC AAGCAGGCAC 
GCTGGAGAGA CACCTAACAG CACTGCCAGT GTCGAGGGAC TTCCTAATCA TGTCCATCAG 
GTGACGGCAG ATGCTGTTTG CAGTCCTAGC CATGGAGATT CTAAGTCACC AGGTTTACTA 
AGTTCAGACA ATCCTCAGCT CTCTGCCTTG TTGATGGGAA AAGCCAATAA CAATGTGGGT 
ACTGGAACCT GTGACAAAGT CAATAACATC CACCCAGCTG TTCATACAAA GACTGATAAT 
TCTGTTGCCT CTTCACCATC TTCAGCCATT TCCACAGCAA CACCTTCTCC AAAATCCACT 
GAGCAGACAA CCACAAACAG TGTTACCAGC CTTAATAGCC CTCACAGTGG GCTACACACA 
ATTAATGGAG AAGGAATGGA GGAATCTCAG AGCCCCATGA AAACAGATGT GCTTCTGATT 
AGCCACAAAC CTAATCCTCA GATCATACCA TCAATGTCTG TATCCATATA CCCCAGCTCA 
GCAGAAGTTC TGAAGGCATG CAGGAATCTA GGTAAAAATG GCTTATCTAA CAGTAGCATT 
TTATTGGATA AATGTCCGCC TCCAAGACCA CCATCTTCAC CATACCCTCC CTTGCCAAAG 
GACAAGTTGA ATCCACCTAC ACCTAGTATT TATTTGGAAA ATAAACGTGA TGCTTTCTTT 
CCTCCATTAC ACCAATTTTG TACAAATCCA AACAACCCTG TTACGGTAAT ACGTGGCCTT 
GCTGGAGCTC TTAAGTTGGA CCTGGGACTT TTCTCTACCA AAACTTTGGT GGAAGCTAAC 
AATGAACATA TGGTGGAAGT GAGGACACAG TTGTTGCAAC CAGCAGATGA AAACTGGGAT 
CCTACTGGAA CAAAGAAAAT CTGGCATTGT GAAAGTAATC GATCTCATAC TACAATTGCT 
AAATATGCAC AGTACCAGGC CTCCTCCTTC CAGGAATCAT TGAGGGAAGA AAATGAGAAA 
AGAAGTCACC ATAAAGACCA TTCAGATAGT GAATCTACAT CTTCAGATAA TTCTGGGAGG 
AGGAGGAAAG GACCCTTTAA AACCATAAAG TTTGGGACCA ACATTGACCT GTCTGATGAC 
AAAAAATGGA AGTTGCAGCT ACATGAGCTG ACTAAACTTC CTGCTTTTGT GCGTGTCGTA 
TCAGCAGGAA ATCTTCTAAG CCACGTTGGT CATACCATAC TGGGCATGAA CACAGTTCAA 
CTGTACATGA AAGTTCCAGG AAGCAGAACA CCAGGTCATC AAGAAAATAA CAACTTCTGT 
TCGGTTAATA TAAATATTGG TCCAGGTGAC TGTGAATGGT TTGTTGTCCC TGAAGGTTAC 
TGGGGTGTTC TGAATGACTT CTGTGAAAAA AATAATTTGA ATTTCCTAAT GGGTTCTTGG 
TGGCCCAACC TTGAAGATCT TTATGAAGCA AATGTTCCAG TGTATAGGTT TATTCAGCGA 
CCTGGAGATT TGGTCTGGAT AAATGCAGGC ACTGTTCATT GGGTTCAGGC TATTGGCTGG 
TGCAACAACA TTGCTTGGAA TGTTGGTCCA CTTACAGCCT GCCAGTATAA ATTGGCAGTG 
GAACGGTACG AATGGAACAA ATTGCAAAGT GTGAAGTCAA TAGTACCCAT GGTTCATCTT 
TCCTGGAATA TGGCACGAAA TATCAAGGTC TCAGATCCAA AGCTTTTTGA AATGATTAAG 
TATTGTCTTC TGAGAACTCT GAAGCAATGT CAGACATTGA GGGAAGCTCT CATTGCTGCA 
GGAAAAGAGA TTATATGGCA TGGGCGGACA AAAGAAGAAC CAGCGCATTA CTGTAGCATT 
TGTGAGGTGG AGGTTTTTGA TCTGCTTTTT GTCACAAATG AGAGCAATTC TCGAAAGACC 
TACATAGTGC ATTGCCAAGA CTGTGCACGA AAAACAAGTG GAAACCTGGA AAATTTTGTG 
GTGCTAGAAC AGTATAAAAT GGAGGACCTG ATGCAAGTCT ATGACCAATT TACATTAGCT 
CCTCCATTAC CATCCTCCTC ATCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005641062.1
CDS200..4426
Translation

Target ORF information:

RefSeq Version XM_005641005.3
Organism Canis lupus familiaris(dog)
Definition Canis lupus familiaris lysine demethylase 6A (KDM6A), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005641005.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTATT CGGGTTCGTG 
AGGTTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAC 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAAT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTACA ATGCATTTCA CTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAT 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG CAATCCATGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAG 
TATCATTCTG CAAAGGAAGC TTATGAACAA CTTTTGCAGA CGGAAAACCT TTCTGCACAA 
GTAAAGGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTTCTGGGA 
GATAAAGCTA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTT GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGCGTGCT ATATCAGCAG CAAAATCAGC CTATGGATGC TTTACAGGCT 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACA 
CTCTACGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACCAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCATGT 
AAACCTCATC ATCCAAATAC TGAACCTGTA TTAGGCCTCA GTCAAACACC AATTTCACAG 
CAGTCCTTGC CACTACACAT GATTCCTTCT AGCCAAGTAG ATGACCTGTC CAGTCCTGCC 
AAGAGGAAAA GAACATCTAG TCCAACAAAG AATACTTCTG ATAATTGGAG TAGTGGCCAT 
GCAGTGTCAC ATCCTCCAGT ACAGCAACAA GCTCATTCGT GGTGTTTGAC ACCACAGAAA 
TTACAGCACT TGGAACAGCT CCGTGCAAAT AGAAATAACT TAAATCCAGC ACAGAAACTG 
ATGCTGGAAC AGCTGGAAAG TCAGTTTGTC TTAATGCAAC AACACCAAAT GAGACAAACA 
GGAGTTGCAC AGGTACGATC TACTGGAATT CCTAATGGGC CAACAGCTGA CTCATCACTG 
CCTACAAACT CAGTCTCTGG CCAGCAGCCA CAGCTTGCTC TGACCAGAGT GCCTAGCGTC 
TCTCAGCCTG GAGTCCGCCC TGCCTGCCCT GGGCAGCCTT TGGCCAACGG ACCCTTTTCT 
GCAGGCCATG TTCCCTGTAG CACATCAAGA ACGCTGGGGA GTACAGACAC TATATTGATA 
GGCAATAATC ACATAACAGG AAGTGGAAGT AATGGAAACG TGCCTTACCT GCAGCGAAAC 
GCACTCACTC TACCTCATAA CCGCACAAAC CTGACCAGCA GCGCAGAGGA GCCGTGGAAA 
AACCAACTAT CTAACTCCAC TCAGGGGCTT CACAAAGGTC AGAGTTCACA TTTGGCAGGT 
CCTAATGGTG AACGACCTCT CTCTTCCACT GGGCCTTCCC AGCATCTCCA GGCAGCTGGC 
TCTGGTATTC AGAATCAGAA TGGACATCCC ACCCTGCCTA GCAATTCAGT AACACAGGGG 
GCTGCTCTCA ATCACCTCTC CTCTCACACT GCTACCTCAG GTGGACAACA AGGCATTACC 
TTAACCAAAG AGAGCAAGCC TTCAGGAAAC ACATCGATGG TGCCTGAAAC AAGCAGGCAC 
GCTGGAGAGA CACCTAACAG CACTGCCAGT GTCGAGGGAC TTCCTAATCA TGTCCATCAG 
GTGACGGCAG ATGCTGTTTG CAGTCCTAGC CATGGAGATT CTAAGTCACC AGGTTTACTA 
AGTTCAGACA ATCCTCAGCT CTCTGCCTTG TTGATGGGAA AAGCCAATAA CAATGTGGGT 
ACTGGAACCT GTGACAAAGT CAATAACATC CACCCAGCTG TTCATACAAA GACTGATAAT 
TCTGTTGCCT CTTCACCATC TTCAGCCATT TCCACAGCAA CACCTTCTCC AAAATCCACT 
GAGCAGACAA CCACAAACAG TGTTACCAGC CTTAATAGCC CTCACAGTGG GCTACACACA 
ATTAATGGAG AAGGAATGGA GGAATCTCAG AGCCCCATGA AAACAGATGT GCTTCTGATT 
AGCCACAAAC CTAATCCTCA GATCATACCA TCAATGTCTG TATCCATATA CCCCAGCTCA 
GCAGAAGTTC TGAAGGCATG CAGGAATCTA GGTAAAAATG GCTTATCTAA CAGTAGCATT 
TTATTGGATA AATGTCCGCC TCCAAGACCA CCATCTTCAC CATACCCTCC CTTGCCAAAG 
GACAAGTTGA ATCCACCTAC ACCTAGTATT TATTTGGAAA ATAAACGTGA TGCTTTCTTT 
CCTCCATTAC ACCAATTTTG TACAAATCCA AACAACCCTG TTACGGTAAT ACGTGGCCTT 
GCTGGAGCTC TTAAGTTGGA CCTGGGACTT TTCTCTACCA AAACTTTGGT GGAAGCTAAC 
AATGAACATA TGGTGGAAGT GAGGACACAG TTGTTGCAAC CAGCAGATGA AAACTGGGAT 
CCTACTGGAA CAAAGAAAAT CTGGCATTGT GAAAGTAATC GATCTCATAC TACAATTGCT 
AAATATGCAC AGTACCAGGC CTCCTCCTTC CAGGAATCAT TGAGGGAAGA AAATGAGAAA 
AGAAGTCACC ATAAAGACCA TTCAGATAGT GAATCTACAT CTTCAGATAA TTCTGGGAGG 
AGGAGGAAAG GACCCTTTAA AACCATAAAG TTTGGGACCA ACATTGACCT GTCTGATGAC 
AAAAAATGGA AGTTGCAGCT ACATGAGCTG ACTAAACTTC CTGCTTTTGT GCGTGTCGTA 
TCAGCAGGAA ATCTTCTAAG CCACGTTGGT CATACCATAC TGGGCATGAA CACAGTTCAA 
CTGTACATGA AAGTTCCAGG AAGCAGAACA CCAGGTCATC AAGAAAATAA CAACTTCTGT 
TCGGTTAATA TAAATATTGG TCCAGGTGAC TGTGAATGGT TTGTTGTCCC TGAAGGTTAC 
TGGGGTGTTC TGAATGACTT CTGTGAAAAA AATAATTTGA ATTTCCTAAT GGGTTCTTGG 
TGGCCCAACC TTGAAGATCT TTATGAAGCA AATGTTCCAG TGTATAGGTT TATTCAGCGA 
CCTGGAGATT TGGTCTGGAT AAATGCAGGC ACTGTTCATT GGGTTCAGGC TATTGGCTGG 
TGCAACAACA TTGCTTGGAA TGTTGGTCCA CTTACAGCCT GCCAGTATAA ATTGGCAGTG 
GAACGGTACG AATGGAACAA ATTGCAAAGT GTGAAGTCAA TAGTACCCAT GGTTCATCTT 
TCCTGGAATA TGGCACGAAA TATCAAGGTC TCAGATCCAA AGCTTTTTGA AATGATTAAG 
TATTGTCTTC TGAGAACTCT GAAGCAATGT CAGACATTGA GGGAAGCTCT CATTGCTGCA 
GGAAAAGAGA TTATATGGCA TGGGCGGACA AAAGAAGAAC CAGCGCATTA CTGTAGCATT 
TGTGAGGTGG AGGTTTTTGA TCTGCTTTTT GTCACAAATG AGAGCAATTC TCGAAAGACC 
TACATAGTGC ATTGCCAAGA CTGTGCACGA AAAACAAGTG GAAACCTGGA AAATTTTGTG 
GTGCTAGAAC AGTATAAAAT GGAGGACCTG ATGCAAGTCT ATGACCAATT TACATTAGCT 
CCTCCATTAC CATCCTCCTC ATCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe31950
Clone ID Related Accession (Same CDS sequence) XM_005641005.3 , XM_005641005.2
Accession Version XM_005641005.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 4227bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2015-09-16
Organism Canis lupus familiaris(Dog)
Product lysine-specific demethylase 6A isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005641005.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTATT CGGGTTCGTG 
AGGTTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAC 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAAT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTACA ATGCATTTCA CTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAT 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG CAATCCATGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAG 
TATCATTCTG CAAAGGAAGC TTATGAACAA CTTTTGCAGA CGGAAAACCT TTCTGCACAA 
GTAAAGGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTTCTGGGA 
GATAAAGCTA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTT GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGCGTGCT ATATCAGCAG CAAAATCAGC CTATGGATGC TTTACAGGCT 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACA 
CTCTACGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACCAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCATGT 
AAACCTCATC ATCCAAATAC TGAACCTGTA TTAGGCCTCA GTCAAACACC AATTTCACAG 
CAGTCCTTGC CACTACACAT GATTCCTTCT AGCCAAGTAG ATGACCTGTC CAGTCCTGCC 
AAGAGGAAAA GAACATCTAG TCCAACAAAG AATACTTCTG ATAATTGGAG TAGTGGCCAT 
GCAGTGTCAC ATCCTCCAGT ACAGCAACAA GCTCATTCGT GGTGTTTGAC ACCACAGAAA 
TTACAGCACT TGGAACAGCT CCGTGCAAAT AGAAATAACT TAAATCCAGC ACAGAAACTG 
ATGCTGGAAC AGCTGGAAAG TCAGTTTGTC TTAATGCAAC AACACCAAAT GAGACAAACA 
GGAGTTGCAC AGGTACGATC TACTGGAATT CCTAATGGGC CAACAGCTGA CTCATCACTG 
CCTACAAACT CAGTCTCTGG CCAGCAGCCA CAGCTTGCTC TGACCAGAGT GCCTAGCGTC 
TCTCAGCCTG GAGTCCGCCC TGCCTGCCCT GGGCAGCCTT TGGCCAACGG ACCCTTTTCT 
GCAGGCCATG TTCCCTGTAG CACATCAAGA ACGCTGGGGA GTACAGACAC TATATTGATA 
GGCAATAATC ACATAACAGG AAGTGGAAGT AATGGAAACG TGCCTTACCT GCAGCGAAAC 
GCACTCACTC TACCTCATAA CCGCACAAAC CTGACCAGCA GCGCAGAGGA GCCGTGGAAA 
AACCAACTAT CTAACTCCAC TCAGGGGCTT CACAAAGGTC AGAGTTCACA TTTGGCAGGT 
CCTAATGGTG AACGACCTCT CTCTTCCACT GGGCCTTCCC AGCATCTCCA GGCAGCTGGC 
TCTGGTATTC AGAATCAGAA TGGACATCCC ACCCTGCCTA GCAATTCAGT AACACAGGGG 
GCTGCTCTCA ATCACCTCTC CTCTCACACT GCTACCTCAG GTGGACAACA AGGCATTACC 
TTAACCAAAG AGAGCAAGCC TTCAGGAAAC ACATCGATGG TGCCTGAAAC AAGCAGGCAC 
GCTGGAGAGA CACCTAACAG CACTGCCAGT GTCGAGGGAC TTCCTAATCA TGTCCATCAG 
GTGACGGCAG ATGCTGTTTG CAGTCCTAGC CATGGAGATT CTAAGTCACC AGGTTTACTA 
AGTTCAGACA ATCCTCAGCT CTCTGCCTTG TTGATGGGAA AAGCCAATAA CAATGTGGGT 
ACTGGAACCT GTGACAAAGT CAATAACATC CACCCAGCTG TTCATACAAA GACTGATAAT 
TCTGTTGCCT CTTCACCATC TTCAGCCATT TCCACAGCAA CACCTTCTCC AAAATCCACT 
GAGCAGACAA CCACAAACAG TGTTACCAGC CTTAATAGCC CTCACAGTGG GCTACACACA 
ATTAATGGAG AAGGAATGGA GGAATCTCAG AGCCCCATGA AAACAGATGT GCTTCTGATT 
AGCCACAAAC CTAATCCTCA GATCATACCA TCAATGTCTG TATCCATATA CCCCAGCTCA 
GCAGAAGTTC TGAAGGCATG CAGGAATCTA GGTAAAAATG GCTTATCTAA CAGTAGCATT 
TTATTGGATA AATGTCCGCC TCCAAGACCA CCATCTTCAC CATACCCTCC CTTGCCAAAG 
GACAAGTTGA ATCCACCTAC ACCTAGTATT TATTTGGAAA ATAAACGTGA TGCTTTCTTT 
CCTCCATTAC ACCAATTTTG TACAAATCCA AACAACCCTG TTACGGTAAT ACGTGGCCTT 
GCTGGAGCTC TTAAGTTGGA CCTGGGACTT TTCTCTACCA AAACTTTGGT GGAAGCTAAC 
AATGAACATA TGGTGGAAGT GAGGACACAG TTGTTGCAAC CAGCAGATGA AAACTGGGAT 
CCTACTGGAA CAAAGAAAAT CTGGCATTGT GAAAGTAATC GATCTCATAC TACAATTGCT 
AAATATGCAC AGTACCAGGC CTCCTCCTTC CAGGAATCAT TGAGGGAAGA AAATGAGAAA 
AGAAGTCACC ATAAAGACCA TTCAGATAGT GAATCTACAT CTTCAGATAA TTCTGGGAGG 
AGGAGGAAAG GACCCTTTAA AACCATAAAG TTTGGGACCA ACATTGACCT GTCTGATGAC 
AAAAAATGGA AGTTGCAGCT ACATGAGCTG ACTAAACTTC CTGCTTTTGT GCGTGTCGTA 
TCAGCAGGAA ATCTTCTAAG CCACGTTGGT CATACCATAC TGGGCATGAA CACAGTTCAA 
CTGTACATGA AAGTTCCAGG AAGCAGAACA CCAGGTCATC AAGAAAATAA CAACTTCTGT 
TCGGTTAATA TAAATATTGG TCCAGGTGAC TGTGAATGGT TTGTTGTCCC TGAAGGTTAC 
TGGGGTGTTC TGAATGACTT CTGTGAAAAA AATAATTTGA ATTTCCTAAT GGGTTCTTGG 
TGGCCCAACC TTGAAGATCT TTATGAAGCA AATGTTCCAG TGTATAGGTT TATTCAGCGA 
CCTGGAGATT TGGTCTGGAT AAATGCAGGC ACTGTTCATT GGGTTCAGGC TATTGGCTGG 
TGCAACAACA TTGCTTGGAA TGTTGGTCCA CTTACAGCCT GCCAGTATAA ATTGGCAGTG 
GAACGGTACG AATGGAACAA ATTGCAAAGT GTGAAGTCAA TAGTACCCAT GGTTCATCTT 
TCCTGGAATA TGGCACGAAA TATCAAGGTC TCAGATCCAA AGCTTTTTGA AATGATTAAG 
TATTGTCTTC TGAGAACTCT GAAGCAATGT CAGACATTGA GGGAAGCTCT CATTGCTGCA 
GGAAAAGAGA TTATATGGCA TGGGCGGACA AAAGAAGAAC CAGCGCATTA CTGTAGCATT 
TGTGAGGTGG AGGTTTTTGA TCTGCTTTTT GTCACAAATG AGAGCAATTC TCGAAAGACC 
TACATAGTGC ATTGCCAAGA CTGTGCACGA AAAACAAGTG GAAACCTGGA AAATTTTGTG 
GTGCTAGAAC AGTATAAAAT GGAGGACCTG ATGCAAGTCT ATGACCAATT TACATTAGCT 
CCTCCATTAC CATCCTCCTC ATCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005641062.1
CDS380..4606
Translation

Target ORF information:

RefSeq Version XM_005641005.2
Organism Canis lupus familiaris(Dog)
Definition Canis lupus familiaris lysine (K)-specific demethylase 6A (KDM6A), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005641005.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTATT CGGGTTCGTG 
AGGTTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAC 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAAT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTACA ATGCATTTCA CTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAT 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG CAATCCATGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAG 
TATCATTCTG CAAAGGAAGC TTATGAACAA CTTTTGCAGA CGGAAAACCT TTCTGCACAA 
GTAAAGGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTTCTGGGA 
GATAAAGCTA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTT GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGCGTGCT ATATCAGCAG CAAAATCAGC CTATGGATGC TTTACAGGCT 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACA 
CTCTACGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACCAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCATGT 
AAACCTCATC ATCCAAATAC TGAACCTGTA TTAGGCCTCA GTCAAACACC AATTTCACAG 
CAGTCCTTGC CACTACACAT GATTCCTTCT AGCCAAGTAG ATGACCTGTC CAGTCCTGCC 
AAGAGGAAAA GAACATCTAG TCCAACAAAG AATACTTCTG ATAATTGGAG TAGTGGCCAT 
GCAGTGTCAC ATCCTCCAGT ACAGCAACAA GCTCATTCGT GGTGTTTGAC ACCACAGAAA 
TTACAGCACT TGGAACAGCT CCGTGCAAAT AGAAATAACT TAAATCCAGC ACAGAAACTG 
ATGCTGGAAC AGCTGGAAAG TCAGTTTGTC TTAATGCAAC AACACCAAAT GAGACAAACA 
GGAGTTGCAC AGGTACGATC TACTGGAATT CCTAATGGGC CAACAGCTGA CTCATCACTG 
CCTACAAACT CAGTCTCTGG CCAGCAGCCA CAGCTTGCTC TGACCAGAGT GCCTAGCGTC 
TCTCAGCCTG GAGTCCGCCC TGCCTGCCCT GGGCAGCCTT TGGCCAACGG ACCCTTTTCT 
GCAGGCCATG TTCCCTGTAG CACATCAAGA ACGCTGGGGA GTACAGACAC TATATTGATA 
GGCAATAATC ACATAACAGG AAGTGGAAGT AATGGAAACG TGCCTTACCT GCAGCGAAAC 
GCACTCACTC TACCTCATAA CCGCACAAAC CTGACCAGCA GCGCAGAGGA GCCGTGGAAA 
AACCAACTAT CTAACTCCAC TCAGGGGCTT CACAAAGGTC AGAGTTCACA TTTGGCAGGT 
CCTAATGGTG AACGACCTCT CTCTTCCACT GGGCCTTCCC AGCATCTCCA GGCAGCTGGC 
TCTGGTATTC AGAATCAGAA TGGACATCCC ACCCTGCCTA GCAATTCAGT AACACAGGGG 
GCTGCTCTCA ATCACCTCTC CTCTCACACT GCTACCTCAG GTGGACAACA AGGCATTACC 
TTAACCAAAG AGAGCAAGCC TTCAGGAAAC ACATCGATGG TGCCTGAAAC AAGCAGGCAC 
GCTGGAGAGA CACCTAACAG CACTGCCAGT GTCGAGGGAC TTCCTAATCA TGTCCATCAG 
GTGACGGCAG ATGCTGTTTG CAGTCCTAGC CATGGAGATT CTAAGTCACC AGGTTTACTA 
AGTTCAGACA ATCCTCAGCT CTCTGCCTTG TTGATGGGAA AAGCCAATAA CAATGTGGGT 
ACTGGAACCT GTGACAAAGT CAATAACATC CACCCAGCTG TTCATACAAA GACTGATAAT 
TCTGTTGCCT CTTCACCATC TTCAGCCATT TCCACAGCAA CACCTTCTCC AAAATCCACT 
GAGCAGACAA CCACAAACAG TGTTACCAGC CTTAATAGCC CTCACAGTGG GCTACACACA 
ATTAATGGAG AAGGAATGGA GGAATCTCAG AGCCCCATGA AAACAGATGT GCTTCTGATT 
AGCCACAAAC CTAATCCTCA GATCATACCA TCAATGTCTG TATCCATATA CCCCAGCTCA 
GCAGAAGTTC TGAAGGCATG CAGGAATCTA GGTAAAAATG GCTTATCTAA CAGTAGCATT 
TTATTGGATA AATGTCCGCC TCCAAGACCA CCATCTTCAC CATACCCTCC CTTGCCAAAG 
GACAAGTTGA ATCCACCTAC ACCTAGTATT TATTTGGAAA ATAAACGTGA TGCTTTCTTT 
CCTCCATTAC ACCAATTTTG TACAAATCCA AACAACCCTG TTACGGTAAT ACGTGGCCTT 
GCTGGAGCTC TTAAGTTGGA CCTGGGACTT TTCTCTACCA AAACTTTGGT GGAAGCTAAC 
AATGAACATA TGGTGGAAGT GAGGACACAG TTGTTGCAAC CAGCAGATGA AAACTGGGAT 
CCTACTGGAA CAAAGAAAAT CTGGCATTGT GAAAGTAATC GATCTCATAC TACAATTGCT 
AAATATGCAC AGTACCAGGC CTCCTCCTTC CAGGAATCAT TGAGGGAAGA AAATGAGAAA 
AGAAGTCACC ATAAAGACCA TTCAGATAGT GAATCTACAT CTTCAGATAA TTCTGGGAGG 
AGGAGGAAAG GACCCTTTAA AACCATAAAG TTTGGGACCA ACATTGACCT GTCTGATGAC 
AAAAAATGGA AGTTGCAGCT ACATGAGCTG ACTAAACTTC CTGCTTTTGT GCGTGTCGTA 
TCAGCAGGAA ATCTTCTAAG CCACGTTGGT CATACCATAC TGGGCATGAA CACAGTTCAA 
CTGTACATGA AAGTTCCAGG AAGCAGAACA CCAGGTCATC AAGAAAATAA CAACTTCTGT 
TCGGTTAATA TAAATATTGG TCCAGGTGAC TGTGAATGGT TTGTTGTCCC TGAAGGTTAC 
TGGGGTGTTC TGAATGACTT CTGTGAAAAA AATAATTTGA ATTTCCTAAT GGGTTCTTGG 
TGGCCCAACC TTGAAGATCT TTATGAAGCA AATGTTCCAG TGTATAGGTT TATTCAGCGA 
CCTGGAGATT TGGTCTGGAT AAATGCAGGC ACTGTTCATT GGGTTCAGGC TATTGGCTGG 
TGCAACAACA TTGCTTGGAA TGTTGGTCCA CTTACAGCCT GCCAGTATAA ATTGGCAGTG 
GAACGGTACG AATGGAACAA ATTGCAAAGT GTGAAGTCAA TAGTACCCAT GGTTCATCTT 
TCCTGGAATA TGGCACGAAA TATCAAGGTC TCAGATCCAA AGCTTTTTGA AATGATTAAG 
TATTGTCTTC TGAGAACTCT GAAGCAATGT CAGACATTGA GGGAAGCTCT CATTGCTGCA 
GGAAAAGAGA TTATATGGCA TGGGCGGACA AAAGAAGAAC CAGCGCATTA CTGTAGCATT 
TGTGAGGTGG AGGTTTTTGA TCTGCTTTTT GTCACAAATG AGAGCAATTC TCGAAAGACC 
TACATAGTGC ATTGCCAAGA CTGTGCACGA AAAACAAGTG GAAACCTGGA AAATTTTGTG 
GTGCTAGAAC AGTATAAAAT GGAGGACCTG ATGCAAGTCT ATGACCAATT TACATTAGCT 
CCTCCATTAC CATCCTCCTC ATCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe31951
Clone ID Related Accession (Same CDS sequence) XM_005641006.2 , XM_005641006.3
Accession Version XM_005641006.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 4206bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2015-09-16
Organism Canis lupus familiaris(Dog)
Product lysine-specific demethylase 6A isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005641006.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTATT CGGGTTCGTG 
AGGTTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAC 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAAT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTACA ATGCATTTCA CTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAT 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG CAATCCATGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAG 
TATCATTCTG CAAAGGAAGC TTATGAACAA CTTTTGCAGA CGGAAAACCT TTCTGCACAA 
GTAAAGGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTTCTGGGA 
GATAAAGCTA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTT GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGCGTGCT ATATCAGCAG CAAAATCAGC CTATGGATGC TTTACAGGCT 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACA 
CTCTACGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACCAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG 
TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG 
GAGGCGTGGA GCCTACCAAT CCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA 
GCACAGCAGA ATACTTCTGA TAATTGGAGT AGTGGCCATG CAGTGTCACA TCCTCCAGTA 
CAGCAACAAG CTCATTCGTG GTGTTTGACA CCACAGAAAT TACAGCACTT GGAACAGCTC 
CGTGCAAATA GAAATAACTT AAATCCAGCA CAGAAACTGA TGCTGGAACA GCTGGAAAGT 
CAGTTTGTCT TAATGCAACA ACACCAAATG AGACAAACAG GAGTTGCACA GGTACGATCT 
ACTGGAATTC CTAATGGGCC AACAGCTGAC TCATCACTGC CTACAAACTC AGTCTCTGGC 
CAGCAGCCAC AGCTTGCTCT GACCAGAGTG CCTAGCGTCT CTCAGCCTGG AGTCCGCCCT 
GCCTGCCCTG GGCAGCCTTT GGCCAACGGA CCCTTTTCTG CAGGCCATGT TCCCTGTAGC 
ACATCAAGAA CGCTGGGGAG TACAGACACT ATATTGATAG GCAATAATCA CATAACAGGA 
AGTGGAAGTA ATGGAAACGT GCCTTACCTG CAGCGAAACG CACTCACTCT ACCTCATAAC 
CGCACAAACC TGACCAGCAG CGCAGAGGAG CCGTGGAAAA ACCAACTATC TAACTCCACT 
CAGGGGCTTC ACAAAGGTCA GAGTTCACAT TTGGCAGGTC CTAATGGTGA ACGACCTCTC 
TCTTCCACTG GGCCTTCCCA GCATCTCCAG GCAGCTGGCT CTGGTATTCA GAATCAGAAT 
GGACATCCCA CCCTGCCTAG CAATTCAGTA ACACAGGGGG CTGCTCTCAA TCACCTCTCC 
TCTCACACTG CTACCTCAGG TGGACAACAA GGCATTACCT TAACCAAAGA GAGCAAGCCT 
TCAGGAAACA CATCGATGGT GCCTGAAACA AGCAGGCACG CTGGAGAGAC ACCTAACAGC 
ACTGCCAGTG TCGAGGGACT TCCTAATCAT GTCCATCAGG TGACGGCAGA TGCTGTTTGC 
AGTCCTAGCC ATGGAGATTC TAAGTCACCA GGTTTACTAA GTTCAGACAA TCCTCAGCTC 
TCTGCCTTGT TGATGGGAAA AGCCAATAAC AATGTGGGTA CTGGAACCTG TGACAAAGTC 
AATAACATCC ACCCAGCTGT TCATACAAAG ACTGATAATT CTGTTGCCTC TTCACCATCT 
TCAGCCATTT CCACAGCAAC ACCTTCTCCA AAATCCACTG AGCAGACAAC CACAAACAGT 
GTTACCAGCC TTAATAGCCC TCACAGTGGG CTACACACAA TTAATGGAGA AGGAATGGAG 
GAATCTCAGA GCCCCATGAA AACAGATGTG CTTCTGATTA GCCACAAACC TAATCCTCAG 
ATCATACCAT CAATGTCTGT ATCCATATAC CCCAGCTCAG CAGAAGTTCT GAAGGCATGC 
AGGAATCTAG GTAAAAATGG CTTATCTAAC AGTAGCATTT TATTGGATAA ATGTCCGCCT 
CCAAGACCAC CATCTTCACC ATACCCTCCC TTGCCAAAGG ACAAGTTGAA TCCACCTACA 
CCTAGTATTT ATTTGGAAAA TAAACGTGAT GCTTTCTTTC CTCCATTACA CCAATTTTGT 
ACAAATCCAA ACAACCCTGT TACGGTAATA CGTGGCCTTG CTGGAGCTCT TAAGTTGGAC 
CTGGGACTTT TCTCTACCAA AACTTTGGTG GAAGCTAACA ATGAACATAT GGTGGAAGTG 
AGGACACAGT TGTTGCAACC AGCAGATGAA AACTGGGATC CTACTGGAAC AAAGAAAATC 
TGGCATTGTG AAAGTAATCG ATCTCATACT ACAATTGCTA AATATGCACA GTACCAGGCC 
TCCTCCTTCC AGGAATCATT GAGGGAAGAA AATGAGAAAA GAAGTCACCA TAAAGACCAT 
TCAGATAGTG AATCTACATC TTCAGATAAT TCTGGGAGGA GGAGGAAAGG ACCCTTTAAA 
ACCATAAAGT TTGGGACCAA CATTGACCTG TCTGATGACA AAAAATGGAA GTTGCAGCTA 
CATGAGCTGA CTAAACTTCC TGCTTTTGTG CGTGTCGTAT CAGCAGGAAA TCTTCTAAGC 
CACGTTGGTC ATACCATACT GGGCATGAAC ACAGTTCAAC TGTACATGAA AGTTCCAGGA 
AGCAGAACAC CAGGTCATCA AGAAAATAAC AACTTCTGTT CGGTTAATAT AAATATTGGT 
CCAGGTGACT GTGAATGGTT TGTTGTCCCT GAAGGTTACT GGGGTGTTCT GAATGACTTC 
TGTGAAAAAA ATAATTTGAA TTTCCTAATG GGTTCTTGGT GGCCCAACCT TGAAGATCTT 
TATGAAGCAA ATGTTCCAGT GTATAGGTTT ATTCAGCGAC CTGGAGATTT GGTCTGGATA 
AATGCAGGCA CTGTTCATTG GGTTCAGGCT ATTGGCTGGT GCAACAACAT TGCTTGGAAT 
GTTGGTCCAC TTACAGCCTG CCAGTATAAA TTGGCAGTGG AACGGTACGA ATGGAACAAA 
TTGCAAAGTG TGAAGTCAAT AGTACCCATG GTTCATCTTT CCTGGAATAT GGCACGAAAT 
ATCAAGGTCT CAGATCCAAA GCTTTTTGAA ATGATTAAGT ATTGTCTTCT GAGAACTCTG 
AAGCAATGTC AGACATTGAG GGAAGCTCTC ATTGCTGCAG GAAAAGAGAT TATATGGCAT 
GGGCGGACAA AAGAAGAACC AGCGCATTAC TGTAGCATTT GTGAGGTGGA GGTTTTTGAT 
CTGCTTTTTG TCACAAATGA GAGCAATTCT CGAAAGACCT ACATAGTGCA TTGCCAAGAC 
TGTGCACGAA AAACAAGTGG AAACCTGGAA AATTTTGTGG TGCTAGAACA GTATAAAATG 
GAGGACCTGA TGCAAGTCTA TGACCAATTT ACATTAGCTC CTCCATTACC ATCCTCCTCA 
TCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005641063.1
CDS380..4585
Translation

Target ORF information:

RefSeq Version XM_005641006.2
Organism Canis lupus familiaris(Dog)
Definition Canis lupus familiaris lysine (K)-specific demethylase 6A (KDM6A), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005641006.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTATT CGGGTTCGTG 
AGGTTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAC 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAAT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTACA ATGCATTTCA CTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAT 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG CAATCCATGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAG 
TATCATTCTG CAAAGGAAGC TTATGAACAA CTTTTGCAGA CGGAAAACCT TTCTGCACAA 
GTAAAGGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTTCTGGGA 
GATAAAGCTA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTT GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGCGTGCT ATATCAGCAG CAAAATCAGC CTATGGATGC TTTACAGGCT 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACA 
CTCTACGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACCAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG 
TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG 
GAGGCGTGGA GCCTACCAAT CCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA 
GCACAGCAGA ATACTTCTGA TAATTGGAGT AGTGGCCATG CAGTGTCACA TCCTCCAGTA 
CAGCAACAAG CTCATTCGTG GTGTTTGACA CCACAGAAAT TACAGCACTT GGAACAGCTC 
CGTGCAAATA GAAATAACTT AAATCCAGCA CAGAAACTGA TGCTGGAACA GCTGGAAAGT 
CAGTTTGTCT TAATGCAACA ACACCAAATG AGACAAACAG GAGTTGCACA GGTACGATCT 
ACTGGAATTC CTAATGGGCC AACAGCTGAC TCATCACTGC CTACAAACTC AGTCTCTGGC 
CAGCAGCCAC AGCTTGCTCT GACCAGAGTG CCTAGCGTCT CTCAGCCTGG AGTCCGCCCT 
GCCTGCCCTG GGCAGCCTTT GGCCAACGGA CCCTTTTCTG CAGGCCATGT TCCCTGTAGC 
ACATCAAGAA CGCTGGGGAG TACAGACACT ATATTGATAG GCAATAATCA CATAACAGGA 
AGTGGAAGTA ATGGAAACGT GCCTTACCTG CAGCGAAACG CACTCACTCT ACCTCATAAC 
CGCACAAACC TGACCAGCAG CGCAGAGGAG CCGTGGAAAA ACCAACTATC TAACTCCACT 
CAGGGGCTTC ACAAAGGTCA GAGTTCACAT TTGGCAGGTC CTAATGGTGA ACGACCTCTC 
TCTTCCACTG GGCCTTCCCA GCATCTCCAG GCAGCTGGCT CTGGTATTCA GAATCAGAAT 
GGACATCCCA CCCTGCCTAG CAATTCAGTA ACACAGGGGG CTGCTCTCAA TCACCTCTCC 
TCTCACACTG CTACCTCAGG TGGACAACAA GGCATTACCT TAACCAAAGA GAGCAAGCCT 
TCAGGAAACA CATCGATGGT GCCTGAAACA AGCAGGCACG CTGGAGAGAC ACCTAACAGC 
ACTGCCAGTG TCGAGGGACT TCCTAATCAT GTCCATCAGG TGACGGCAGA TGCTGTTTGC 
AGTCCTAGCC ATGGAGATTC TAAGTCACCA GGTTTACTAA GTTCAGACAA TCCTCAGCTC 
TCTGCCTTGT TGATGGGAAA AGCCAATAAC AATGTGGGTA CTGGAACCTG TGACAAAGTC 
AATAACATCC ACCCAGCTGT TCATACAAAG ACTGATAATT CTGTTGCCTC TTCACCATCT 
TCAGCCATTT CCACAGCAAC ACCTTCTCCA AAATCCACTG AGCAGACAAC CACAAACAGT 
GTTACCAGCC TTAATAGCCC TCACAGTGGG CTACACACAA TTAATGGAGA AGGAATGGAG 
GAATCTCAGA GCCCCATGAA AACAGATGTG CTTCTGATTA GCCACAAACC TAATCCTCAG 
ATCATACCAT CAATGTCTGT ATCCATATAC CCCAGCTCAG CAGAAGTTCT GAAGGCATGC 
AGGAATCTAG GTAAAAATGG CTTATCTAAC AGTAGCATTT TATTGGATAA ATGTCCGCCT 
CCAAGACCAC CATCTTCACC ATACCCTCCC TTGCCAAAGG ACAAGTTGAA TCCACCTACA 
CCTAGTATTT ATTTGGAAAA TAAACGTGAT GCTTTCTTTC CTCCATTACA CCAATTTTGT 
ACAAATCCAA ACAACCCTGT TACGGTAATA CGTGGCCTTG CTGGAGCTCT TAAGTTGGAC 
CTGGGACTTT TCTCTACCAA AACTTTGGTG GAAGCTAACA ATGAACATAT GGTGGAAGTG 
AGGACACAGT TGTTGCAACC AGCAGATGAA AACTGGGATC CTACTGGAAC AAAGAAAATC 
TGGCATTGTG AAAGTAATCG ATCTCATACT ACAATTGCTA AATATGCACA GTACCAGGCC 
TCCTCCTTCC AGGAATCATT GAGGGAAGAA AATGAGAAAA GAAGTCACCA TAAAGACCAT 
TCAGATAGTG AATCTACATC TTCAGATAAT TCTGGGAGGA GGAGGAAAGG ACCCTTTAAA 
ACCATAAAGT TTGGGACCAA CATTGACCTG TCTGATGACA AAAAATGGAA GTTGCAGCTA 
CATGAGCTGA CTAAACTTCC TGCTTTTGTG CGTGTCGTAT CAGCAGGAAA TCTTCTAAGC 
CACGTTGGTC ATACCATACT GGGCATGAAC ACAGTTCAAC TGTACATGAA AGTTCCAGGA 
AGCAGAACAC CAGGTCATCA AGAAAATAAC AACTTCTGTT CGGTTAATAT AAATATTGGT 
CCAGGTGACT GTGAATGGTT TGTTGTCCCT GAAGGTTACT GGGGTGTTCT GAATGACTTC 
TGTGAAAAAA ATAATTTGAA TTTCCTAATG GGTTCTTGGT GGCCCAACCT TGAAGATCTT 
TATGAAGCAA ATGTTCCAGT GTATAGGTTT ATTCAGCGAC CTGGAGATTT GGTCTGGATA 
AATGCAGGCA CTGTTCATTG GGTTCAGGCT ATTGGCTGGT GCAACAACAT TGCTTGGAAT 
GTTGGTCCAC TTACAGCCTG CCAGTATAAA TTGGCAGTGG AACGGTACGA ATGGAACAAA 
TTGCAAAGTG TGAAGTCAAT AGTACCCATG GTTCATCTTT CCTGGAATAT GGCACGAAAT 
ATCAAGGTCT CAGATCCAAA GCTTTTTGAA ATGATTAAGT ATTGTCTTCT GAGAACTCTG 
AAGCAATGTC AGACATTGAG GGAAGCTCTC ATTGCTGCAG GAAAAGAGAT TATATGGCAT 
GGGCGGACAA AAGAAGAACC AGCGCATTAC TGTAGCATTT GTGAGGTGGA GGTTTTTGAT 
CTGCTTTTTG TCACAAATGA GAGCAATTCT CGAAAGACCT ACATAGTGCA TTGCCAAGAC 
TGTGCACGAA AAACAAGTGG AAACCTGGAA AATTTTGTGG TGCTAGAACA GTATAAAATG 
GAGGACCTGA TGCAAGTCTA TGACCAATTT ACATTAGCTC CTCCATTACC ATCCTCCTCA 
TCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe31951
Clone ID Related Accession (Same CDS sequence) XM_005641006.2 , XM_005641006.3
Accession Version XM_005641006.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 4206bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-09-04
Organism Canis lupus familiaris(dog)
Product lysine-specific demethylase 6A isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005641006.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTATT CGGGTTCGTG 
AGGTTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAC 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAAT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTACA ATGCATTTCA CTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAT 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG CAATCCATGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAG 
TATCATTCTG CAAAGGAAGC TTATGAACAA CTTTTGCAGA CGGAAAACCT TTCTGCACAA 
GTAAAGGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTTCTGGGA 
GATAAAGCTA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTT GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGCGTGCT ATATCAGCAG CAAAATCAGC CTATGGATGC TTTACAGGCT 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACA 
CTCTACGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACCAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG 
TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG 
GAGGCGTGGA GCCTACCAAT CCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA 
GCACAGCAGA ATACTTCTGA TAATTGGAGT AGTGGCCATG CAGTGTCACA TCCTCCAGTA 
CAGCAACAAG CTCATTCGTG GTGTTTGACA CCACAGAAAT TACAGCACTT GGAACAGCTC 
CGTGCAAATA GAAATAACTT AAATCCAGCA CAGAAACTGA TGCTGGAACA GCTGGAAAGT 
CAGTTTGTCT TAATGCAACA ACACCAAATG AGACAAACAG GAGTTGCACA GGTACGATCT 
ACTGGAATTC CTAATGGGCC AACAGCTGAC TCATCACTGC CTACAAACTC AGTCTCTGGC 
CAGCAGCCAC AGCTTGCTCT GACCAGAGTG CCTAGCGTCT CTCAGCCTGG AGTCCGCCCT 
GCCTGCCCTG GGCAGCCTTT GGCCAACGGA CCCTTTTCTG CAGGCCATGT TCCCTGTAGC 
ACATCAAGAA CGCTGGGGAG TACAGACACT ATATTGATAG GCAATAATCA CATAACAGGA 
AGTGGAAGTA ATGGAAACGT GCCTTACCTG CAGCGAAACG CACTCACTCT ACCTCATAAC 
CGCACAAACC TGACCAGCAG CGCAGAGGAG CCGTGGAAAA ACCAACTATC TAACTCCACT 
CAGGGGCTTC ACAAAGGTCA GAGTTCACAT TTGGCAGGTC CTAATGGTGA ACGACCTCTC 
TCTTCCACTG GGCCTTCCCA GCATCTCCAG GCAGCTGGCT CTGGTATTCA GAATCAGAAT 
GGACATCCCA CCCTGCCTAG CAATTCAGTA ACACAGGGGG CTGCTCTCAA TCACCTCTCC 
TCTCACACTG CTACCTCAGG TGGACAACAA GGCATTACCT TAACCAAAGA GAGCAAGCCT 
TCAGGAAACA CATCGATGGT GCCTGAAACA AGCAGGCACG CTGGAGAGAC ACCTAACAGC 
ACTGCCAGTG TCGAGGGACT TCCTAATCAT GTCCATCAGG TGACGGCAGA TGCTGTTTGC 
AGTCCTAGCC ATGGAGATTC TAAGTCACCA GGTTTACTAA GTTCAGACAA TCCTCAGCTC 
TCTGCCTTGT TGATGGGAAA AGCCAATAAC AATGTGGGTA CTGGAACCTG TGACAAAGTC 
AATAACATCC ACCCAGCTGT TCATACAAAG ACTGATAATT CTGTTGCCTC TTCACCATCT 
TCAGCCATTT CCACAGCAAC ACCTTCTCCA AAATCCACTG AGCAGACAAC CACAAACAGT 
GTTACCAGCC TTAATAGCCC TCACAGTGGG CTACACACAA TTAATGGAGA AGGAATGGAG 
GAATCTCAGA GCCCCATGAA AACAGATGTG CTTCTGATTA GCCACAAACC TAATCCTCAG 
ATCATACCAT CAATGTCTGT ATCCATATAC CCCAGCTCAG CAGAAGTTCT GAAGGCATGC 
AGGAATCTAG GTAAAAATGG CTTATCTAAC AGTAGCATTT TATTGGATAA ATGTCCGCCT 
CCAAGACCAC CATCTTCACC ATACCCTCCC TTGCCAAAGG ACAAGTTGAA TCCACCTACA 
CCTAGTATTT ATTTGGAAAA TAAACGTGAT GCTTTCTTTC CTCCATTACA CCAATTTTGT 
ACAAATCCAA ACAACCCTGT TACGGTAATA CGTGGCCTTG CTGGAGCTCT TAAGTTGGAC 
CTGGGACTTT TCTCTACCAA AACTTTGGTG GAAGCTAACA ATGAACATAT GGTGGAAGTG 
AGGACACAGT TGTTGCAACC AGCAGATGAA AACTGGGATC CTACTGGAAC AAAGAAAATC 
TGGCATTGTG AAAGTAATCG ATCTCATACT ACAATTGCTA AATATGCACA GTACCAGGCC 
TCCTCCTTCC AGGAATCATT GAGGGAAGAA AATGAGAAAA GAAGTCACCA TAAAGACCAT 
TCAGATAGTG AATCTACATC TTCAGATAAT TCTGGGAGGA GGAGGAAAGG ACCCTTTAAA 
ACCATAAAGT TTGGGACCAA CATTGACCTG TCTGATGACA AAAAATGGAA GTTGCAGCTA 
CATGAGCTGA CTAAACTTCC TGCTTTTGTG CGTGTCGTAT CAGCAGGAAA TCTTCTAAGC 
CACGTTGGTC ATACCATACT GGGCATGAAC ACAGTTCAAC TGTACATGAA AGTTCCAGGA 
AGCAGAACAC CAGGTCATCA AGAAAATAAC AACTTCTGTT CGGTTAATAT AAATATTGGT 
CCAGGTGACT GTGAATGGTT TGTTGTCCCT GAAGGTTACT GGGGTGTTCT GAATGACTTC 
TGTGAAAAAA ATAATTTGAA TTTCCTAATG GGTTCTTGGT GGCCCAACCT TGAAGATCTT 
TATGAAGCAA ATGTTCCAGT GTATAGGTTT ATTCAGCGAC CTGGAGATTT GGTCTGGATA 
AATGCAGGCA CTGTTCATTG GGTTCAGGCT ATTGGCTGGT GCAACAACAT TGCTTGGAAT 
GTTGGTCCAC TTACAGCCTG CCAGTATAAA TTGGCAGTGG AACGGTACGA ATGGAACAAA 
TTGCAAAGTG TGAAGTCAAT AGTACCCATG GTTCATCTTT CCTGGAATAT GGCACGAAAT 
ATCAAGGTCT CAGATCCAAA GCTTTTTGAA ATGATTAAGT ATTGTCTTCT GAGAACTCTG 
AAGCAATGTC AGACATTGAG GGAAGCTCTC ATTGCTGCAG GAAAAGAGAT TATATGGCAT 
GGGCGGACAA AAGAAGAACC AGCGCATTAC TGTAGCATTT GTGAGGTGGA GGTTTTTGAT 
CTGCTTTTTG TCACAAATGA GAGCAATTCT CGAAAGACCT ACATAGTGCA TTGCCAAGAC 
TGTGCACGAA AAACAAGTGG AAACCTGGAA AATTTTGTGG TGCTAGAACA GTATAAAATG 
GAGGACCTGA TGCAAGTCTA TGACCAATTT ACATTAGCTC CTCCATTACC ATCCTCCTCA 
TCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005641063.1
CDS200..4405
Translation

Target ORF information:

RefSeq Version XM_005641006.3
Organism Canis lupus familiaris(dog)
Definition Canis lupus familiaris lysine demethylase 6A (KDM6A), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005641006.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTATT CGGGTTCGTG 
AGGTTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAC 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAAT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTACA ATGCATTTCA CTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAT 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG CAATCCATGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAG 
TATCATTCTG CAAAGGAAGC TTATGAACAA CTTTTGCAGA CGGAAAACCT TTCTGCACAA 
GTAAAGGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTTCTGGGA 
GATAAAGCTA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTT GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGCGTGCT ATATCAGCAG CAAAATCAGC CTATGGATGC TTTACAGGCT 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACA 
CTCTACGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACCAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG 
TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG 
GAGGCGTGGA GCCTACCAAT CCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA 
GCACAGCAGA ATACTTCTGA TAATTGGAGT AGTGGCCATG CAGTGTCACA TCCTCCAGTA 
CAGCAACAAG CTCATTCGTG GTGTTTGACA CCACAGAAAT TACAGCACTT GGAACAGCTC 
CGTGCAAATA GAAATAACTT AAATCCAGCA CAGAAACTGA TGCTGGAACA GCTGGAAAGT 
CAGTTTGTCT TAATGCAACA ACACCAAATG AGACAAACAG GAGTTGCACA GGTACGATCT 
ACTGGAATTC CTAATGGGCC AACAGCTGAC TCATCACTGC CTACAAACTC AGTCTCTGGC 
CAGCAGCCAC AGCTTGCTCT GACCAGAGTG CCTAGCGTCT CTCAGCCTGG AGTCCGCCCT 
GCCTGCCCTG GGCAGCCTTT GGCCAACGGA CCCTTTTCTG CAGGCCATGT TCCCTGTAGC 
ACATCAAGAA CGCTGGGGAG TACAGACACT ATATTGATAG GCAATAATCA CATAACAGGA 
AGTGGAAGTA ATGGAAACGT GCCTTACCTG CAGCGAAACG CACTCACTCT ACCTCATAAC 
CGCACAAACC TGACCAGCAG CGCAGAGGAG CCGTGGAAAA ACCAACTATC TAACTCCACT 
CAGGGGCTTC ACAAAGGTCA GAGTTCACAT TTGGCAGGTC CTAATGGTGA ACGACCTCTC 
TCTTCCACTG GGCCTTCCCA GCATCTCCAG GCAGCTGGCT CTGGTATTCA GAATCAGAAT 
GGACATCCCA CCCTGCCTAG CAATTCAGTA ACACAGGGGG CTGCTCTCAA TCACCTCTCC 
TCTCACACTG CTACCTCAGG TGGACAACAA GGCATTACCT TAACCAAAGA GAGCAAGCCT 
TCAGGAAACA CATCGATGGT GCCTGAAACA AGCAGGCACG CTGGAGAGAC ACCTAACAGC 
ACTGCCAGTG TCGAGGGACT TCCTAATCAT GTCCATCAGG TGACGGCAGA TGCTGTTTGC 
AGTCCTAGCC ATGGAGATTC TAAGTCACCA GGTTTACTAA GTTCAGACAA TCCTCAGCTC 
TCTGCCTTGT TGATGGGAAA AGCCAATAAC AATGTGGGTA CTGGAACCTG TGACAAAGTC 
AATAACATCC ACCCAGCTGT TCATACAAAG ACTGATAATT CTGTTGCCTC TTCACCATCT 
TCAGCCATTT CCACAGCAAC ACCTTCTCCA AAATCCACTG AGCAGACAAC CACAAACAGT 
GTTACCAGCC TTAATAGCCC TCACAGTGGG CTACACACAA TTAATGGAGA AGGAATGGAG 
GAATCTCAGA GCCCCATGAA AACAGATGTG CTTCTGATTA GCCACAAACC TAATCCTCAG 
ATCATACCAT CAATGTCTGT ATCCATATAC CCCAGCTCAG CAGAAGTTCT GAAGGCATGC 
AGGAATCTAG GTAAAAATGG CTTATCTAAC AGTAGCATTT TATTGGATAA ATGTCCGCCT 
CCAAGACCAC CATCTTCACC ATACCCTCCC TTGCCAAAGG ACAAGTTGAA TCCACCTACA 
CCTAGTATTT ATTTGGAAAA TAAACGTGAT GCTTTCTTTC CTCCATTACA CCAATTTTGT 
ACAAATCCAA ACAACCCTGT TACGGTAATA CGTGGCCTTG CTGGAGCTCT TAAGTTGGAC 
CTGGGACTTT TCTCTACCAA AACTTTGGTG GAAGCTAACA ATGAACATAT GGTGGAAGTG 
AGGACACAGT TGTTGCAACC AGCAGATGAA AACTGGGATC CTACTGGAAC AAAGAAAATC 
TGGCATTGTG AAAGTAATCG ATCTCATACT ACAATTGCTA AATATGCACA GTACCAGGCC 
TCCTCCTTCC AGGAATCATT GAGGGAAGAA AATGAGAAAA GAAGTCACCA TAAAGACCAT 
TCAGATAGTG AATCTACATC TTCAGATAAT TCTGGGAGGA GGAGGAAAGG ACCCTTTAAA 
ACCATAAAGT TTGGGACCAA CATTGACCTG TCTGATGACA AAAAATGGAA GTTGCAGCTA 
CATGAGCTGA CTAAACTTCC TGCTTTTGTG CGTGTCGTAT CAGCAGGAAA TCTTCTAAGC 
CACGTTGGTC ATACCATACT GGGCATGAAC ACAGTTCAAC TGTACATGAA AGTTCCAGGA 
AGCAGAACAC CAGGTCATCA AGAAAATAAC AACTTCTGTT CGGTTAATAT AAATATTGGT 
CCAGGTGACT GTGAATGGTT TGTTGTCCCT GAAGGTTACT GGGGTGTTCT GAATGACTTC 
TGTGAAAAAA ATAATTTGAA TTTCCTAATG GGTTCTTGGT GGCCCAACCT TGAAGATCTT 
TATGAAGCAA ATGTTCCAGT GTATAGGTTT ATTCAGCGAC CTGGAGATTT GGTCTGGATA 
AATGCAGGCA CTGTTCATTG GGTTCAGGCT ATTGGCTGGT GCAACAACAT TGCTTGGAAT 
GTTGGTCCAC TTACAGCCTG CCAGTATAAA TTGGCAGTGG AACGGTACGA ATGGAACAAA 
TTGCAAAGTG TGAAGTCAAT AGTACCCATG GTTCATCTTT CCTGGAATAT GGCACGAAAT 
ATCAAGGTCT CAGATCCAAA GCTTTTTGAA ATGATTAAGT ATTGTCTTCT GAGAACTCTG 
AAGCAATGTC AGACATTGAG GGAAGCTCTC ATTGCTGCAG GAAAAGAGAT TATATGGCAT 
GGGCGGACAA AAGAAGAACC AGCGCATTAC TGTAGCATTT GTGAGGTGGA GGTTTTTGAT 
CTGCTTTTTG TCACAAATGA GAGCAATTCT CGAAAGACCT ACATAGTGCA TTGCCAAGAC 
TGTGCACGAA AAACAAGTGG AAACCTGGAA AATTTTGTGG TGCTAGAACA GTATAAAATG 
GAGGACCTGA TGCAAGTCTA TGACCAATTT ACATTAGCTC CTCCATTACC ATCCTCCTCA 
TCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe31952
Clone ID Related Accession (Same CDS sequence) XM_005641007.2
Accession Version XM_005641007.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 4071bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2015-09-16
Organism Canis lupus familiaris(Dog)
Product lysine-specific demethylase 6A isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005641007.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTATT CGGGTTCGTG 
AGGTTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAC 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAAT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTACA ATGCATTTCA CTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAT 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG CAATCCATGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAG 
TATCATTCTG CAAAGGAAGC TTATGAACAA CTTTTGCAGA CGGAAAACCT TTCTGCACAA 
GTAAAGGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTTCTGGGA 
GATAAAGCTA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTT GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGCGTGCT ATATCAGCAG CAAAATCAGC CTATGGATGC TTTACAGGCT 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACA 
CTCTACGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACCAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGAATACT 
TCTGATAATT GGAGTAGTGG CCATGCAGTG TCACATCCTC CAGTACAGCA ACAAGCTCAT 
TCGTGGTGTT TGACACCACA GAAATTACAG CACTTGGAAC AGCTCCGTGC AAATAGAAAT 
AACTTAAATC CAGCACAGAA ACTGATGCTG GAACAGCTGG AAAGTCAGTT TGTCTTAATG 
CAACAACACC AAATGAGACA AACAGGAGTT GCACAGGTAC GATCTACTGG AATTCCTAAT 
GGGCCAACAG CTGACTCATC ACTGCCTACA AACTCAGTCT CTGGCCAGCA GCCACAGCTT 
GCTCTGACCA GAGTGCCTAG CGTCTCTCAG CCTGGAGTCC GCCCTGCCTG CCCTGGGCAG 
CCTTTGGCCA ACGGACCCTT TTCTGCAGGC CATGTTCCCT GTAGCACATC AAGAACGCTG 
GGGAGTACAG ACACTATATT GATAGGCAAT AATCACATAA CAGGAAGTGG AAGTAATGGA 
AACGTGCCTT ACCTGCAGCG AAACGCACTC ACTCTACCTC ATAACCGCAC AAACCTGACC 
AGCAGCGCAG AGGAGCCGTG GAAAAACCAA CTATCTAACT CCACTCAGGG GCTTCACAAA 
GGTCAGAGTT CACATTTGGC AGGTCCTAAT GGTGAACGAC CTCTCTCTTC CACTGGGCCT 
TCCCAGCATC TCCAGGCAGC TGGCTCTGGT ATTCAGAATC AGAATGGACA TCCCACCCTG 
CCTAGCAATT CAGTAACACA GGGGGCTGCT CTCAATCACC TCTCCTCTCA CACTGCTACC 
TCAGGTGGAC AACAAGGCAT TACCTTAACC AAAGAGAGCA AGCCTTCAGG AAACACATCG 
ATGGTGCCTG AAACAAGCAG GCACGCTGGA GAGACACCTA ACAGCACTGC CAGTGTCGAG 
GGACTTCCTA ATCATGTCCA TCAGGTGACG GCAGATGCTG TTTGCAGTCC TAGCCATGGA 
GATTCTAAGT CACCAGGTTT ACTAAGTTCA GACAATCCTC AGCTCTCTGC CTTGTTGATG 
GGAAAAGCCA ATAACAATGT GGGTACTGGA ACCTGTGACA AAGTCAATAA CATCCACCCA 
GCTGTTCATA CAAAGACTGA TAATTCTGTT GCCTCTTCAC CATCTTCAGC CATTTCCACA 
GCAACACCTT CTCCAAAATC CACTGAGCAG ACAACCACAA ACAGTGTTAC CAGCCTTAAT 
AGCCCTCACA GTGGGCTACA CACAATTAAT GGAGAAGGAA TGGAGGAATC TCAGAGCCCC 
ATGAAAACAG ATGTGCTTCT GATTAGCCAC AAACCTAATC CTCAGATCAT ACCATCAATG 
TCTGTATCCA TATACCCCAG CTCAGCAGAA GTTCTGAAGG CATGCAGGAA TCTAGGTAAA 
AATGGCTTAT CTAACAGTAG CATTTTATTG GATAAATGTC CGCCTCCAAG ACCACCATCT 
TCACCATACC CTCCCTTGCC AAAGGACAAG TTGAATCCAC CTACACCTAG TATTTATTTG 
GAAAATAAAC GTGATGCTTT CTTTCCTCCA TTACACCAAT TTTGTACAAA TCCAAACAAC 
CCTGTTACGG TAATACGTGG CCTTGCTGGA GCTCTTAAGT TGGACCTGGG ACTTTTCTCT 
ACCAAAACTT TGGTGGAAGC TAACAATGAA CATATGGTGG AAGTGAGGAC ACAGTTGTTG 
CAACCAGCAG ATGAAAACTG GGATCCTACT GGAACAAAGA AAATCTGGCA TTGTGAAAGT 
AATCGATCTC ATACTACAAT TGCTAAATAT GCACAGTACC AGGCCTCCTC CTTCCAGGAA 
TCATTGAGGG AAGAAAATGA GAAAAGAAGT CACCATAAAG ACCATTCAGA TAGTGAATCT 
ACATCTTCAG ATAATTCTGG GAGGAGGAGG AAAGGACCCT TTAAAACCAT AAAGTTTGGG 
ACCAACATTG ACCTGTCTGA TGACAAAAAA TGGAAGTTGC AGCTACATGA GCTGACTAAA 
CTTCCTGCTT TTGTGCGTGT CGTATCAGCA GGAAATCTTC TAAGCCACGT TGGTCATACC 
ATACTGGGCA TGAACACAGT TCAACTGTAC ATGAAAGTTC CAGGAAGCAG AACACCAGGT 
CATCAAGAAA ATAACAACTT CTGTTCGGTT AATATAAATA TTGGTCCAGG TGACTGTGAA 
TGGTTTGTTG TCCCTGAAGG TTACTGGGGT GTTCTGAATG ACTTCTGTGA AAAAAATAAT 
TTGAATTTCC TAATGGGTTC TTGGTGGCCC AACCTTGAAG ATCTTTATGA AGCAAATGTT 
CCAGTGTATA GGTTTATTCA GCGACCTGGA GATTTGGTCT GGATAAATGC AGGCACTGTT 
CATTGGGTTC AGGCTATTGG CTGGTGCAAC AACATTGCTT GGAATGTTGG TCCACTTACA 
GCCTGCCAGT ATAAATTGGC AGTGGAACGG TACGAATGGA ACAAATTGCA AAGTGTGAAG 
TCAATAGTAC CCATGGTTCA TCTTTCCTGG AATATGGCAC GAAATATCAA GGTCTCAGAT 
CCAAAGCTTT TTGAAATGAT TAAGTATTGT CTTCTGAGAA CTCTGAAGCA ATGTCAGACA 
TTGAGGGAAG CTCTCATTGC TGCAGGAAAA GAGATTATAT GGCATGGGCG GACAAAAGAA 
GAACCAGCGC ATTACTGTAG CATTTGTGAG GTGGAGGTTT TTGATCTGCT TTTTGTCACA 
AATGAGAGCA ATTCTCGAAA GACCTACATA GTGCATTGCC AAGACTGTGC ACGAAAAACA 
AGTGGAAACC TGGAAAATTT TGTGGTGCTA GAACAGTATA AAATGGAGGA CCTGATGCAA 
GTCTATGACC AATTTACATT AGCTCCTCCA TTACCATCCT CCTCATCTTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005641064.1
CDS380..4450
Translation

Target ORF information:

RefSeq Version XM_005641007.2
Organism Canis lupus familiaris(Dog)
Definition Canis lupus familiaris lysine (K)-specific demethylase 6A (KDM6A), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005641007.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTATT CGGGTTCGTG 
AGGTTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAC 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAAT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTACA ATGCATTTCA CTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAT 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG CAATCCATGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAG 
TATCATTCTG CAAAGGAAGC TTATGAACAA CTTTTGCAGA CGGAAAACCT TTCTGCACAA 
GTAAAGGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTTCTGGGA 
GATAAAGCTA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTT GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGCGTGCT ATATCAGCAG CAAAATCAGC CTATGGATGC TTTACAGGCT 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACA 
CTCTACGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACCAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGAATACT 
TCTGATAATT GGAGTAGTGG CCATGCAGTG TCACATCCTC CAGTACAGCA ACAAGCTCAT 
TCGTGGTGTT TGACACCACA GAAATTACAG CACTTGGAAC AGCTCCGTGC AAATAGAAAT 
AACTTAAATC CAGCACAGAA ACTGATGCTG GAACAGCTGG AAAGTCAGTT TGTCTTAATG 
CAACAACACC AAATGAGACA AACAGGAGTT GCACAGGTAC GATCTACTGG AATTCCTAAT 
GGGCCAACAG CTGACTCATC ACTGCCTACA AACTCAGTCT CTGGCCAGCA GCCACAGCTT 
GCTCTGACCA GAGTGCCTAG CGTCTCTCAG CCTGGAGTCC GCCCTGCCTG CCCTGGGCAG 
CCTTTGGCCA ACGGACCCTT TTCTGCAGGC CATGTTCCCT GTAGCACATC AAGAACGCTG 
GGGAGTACAG ACACTATATT GATAGGCAAT AATCACATAA CAGGAAGTGG AAGTAATGGA 
AACGTGCCTT ACCTGCAGCG AAACGCACTC ACTCTACCTC ATAACCGCAC AAACCTGACC 
AGCAGCGCAG AGGAGCCGTG GAAAAACCAA CTATCTAACT CCACTCAGGG GCTTCACAAA 
GGTCAGAGTT CACATTTGGC AGGTCCTAAT GGTGAACGAC CTCTCTCTTC CACTGGGCCT 
TCCCAGCATC TCCAGGCAGC TGGCTCTGGT ATTCAGAATC AGAATGGACA TCCCACCCTG 
CCTAGCAATT CAGTAACACA GGGGGCTGCT CTCAATCACC TCTCCTCTCA CACTGCTACC 
TCAGGTGGAC AACAAGGCAT TACCTTAACC AAAGAGAGCA AGCCTTCAGG AAACACATCG 
ATGGTGCCTG AAACAAGCAG GCACGCTGGA GAGACACCTA ACAGCACTGC CAGTGTCGAG 
GGACTTCCTA ATCATGTCCA TCAGGTGACG GCAGATGCTG TTTGCAGTCC TAGCCATGGA 
GATTCTAAGT CACCAGGTTT ACTAAGTTCA GACAATCCTC AGCTCTCTGC CTTGTTGATG 
GGAAAAGCCA ATAACAATGT GGGTACTGGA ACCTGTGACA AAGTCAATAA CATCCACCCA 
GCTGTTCATA CAAAGACTGA TAATTCTGTT GCCTCTTCAC CATCTTCAGC CATTTCCACA 
GCAACACCTT CTCCAAAATC CACTGAGCAG ACAACCACAA ACAGTGTTAC CAGCCTTAAT 
AGCCCTCACA GTGGGCTACA CACAATTAAT GGAGAAGGAA TGGAGGAATC TCAGAGCCCC 
ATGAAAACAG ATGTGCTTCT GATTAGCCAC AAACCTAATC CTCAGATCAT ACCATCAATG 
TCTGTATCCA TATACCCCAG CTCAGCAGAA GTTCTGAAGG CATGCAGGAA TCTAGGTAAA 
AATGGCTTAT CTAACAGTAG CATTTTATTG GATAAATGTC CGCCTCCAAG ACCACCATCT 
TCACCATACC CTCCCTTGCC AAAGGACAAG TTGAATCCAC CTACACCTAG TATTTATTTG 
GAAAATAAAC GTGATGCTTT CTTTCCTCCA TTACACCAAT TTTGTACAAA TCCAAACAAC 
CCTGTTACGG TAATACGTGG CCTTGCTGGA GCTCTTAAGT TGGACCTGGG ACTTTTCTCT 
ACCAAAACTT TGGTGGAAGC TAACAATGAA CATATGGTGG AAGTGAGGAC ACAGTTGTTG 
CAACCAGCAG ATGAAAACTG GGATCCTACT GGAACAAAGA AAATCTGGCA TTGTGAAAGT 
AATCGATCTC ATACTACAAT TGCTAAATAT GCACAGTACC AGGCCTCCTC CTTCCAGGAA 
TCATTGAGGG AAGAAAATGA GAAAAGAAGT CACCATAAAG ACCATTCAGA TAGTGAATCT 
ACATCTTCAG ATAATTCTGG GAGGAGGAGG AAAGGACCCT TTAAAACCAT AAAGTTTGGG 
ACCAACATTG ACCTGTCTGA TGACAAAAAA TGGAAGTTGC AGCTACATGA GCTGACTAAA 
CTTCCTGCTT TTGTGCGTGT CGTATCAGCA GGAAATCTTC TAAGCCACGT TGGTCATACC 
ATACTGGGCA TGAACACAGT TCAACTGTAC ATGAAAGTTC CAGGAAGCAG AACACCAGGT 
CATCAAGAAA ATAACAACTT CTGTTCGGTT AATATAAATA TTGGTCCAGG TGACTGTGAA 
TGGTTTGTTG TCCCTGAAGG TTACTGGGGT GTTCTGAATG ACTTCTGTGA AAAAAATAAT 
TTGAATTTCC TAATGGGTTC TTGGTGGCCC AACCTTGAAG ATCTTTATGA AGCAAATGTT 
CCAGTGTATA GGTTTATTCA GCGACCTGGA GATTTGGTCT GGATAAATGC AGGCACTGTT 
CATTGGGTTC AGGCTATTGG CTGGTGCAAC AACATTGCTT GGAATGTTGG TCCACTTACA 
GCCTGCCAGT ATAAATTGGC AGTGGAACGG TACGAATGGA ACAAATTGCA AAGTGTGAAG 
TCAATAGTAC CCATGGTTCA TCTTTCCTGG AATATGGCAC GAAATATCAA GGTCTCAGAT 
CCAAAGCTTT TTGAAATGAT TAAGTATTGT CTTCTGAGAA CTCTGAAGCA ATGTCAGACA 
TTGAGGGAAG CTCTCATTGC TGCAGGAAAA GAGATTATAT GGCATGGGCG GACAAAAGAA 
GAACCAGCGC ATTACTGTAG CATTTGTGAG GTGGAGGTTT TTGATCTGCT TTTTGTCACA 
AATGAGAGCA ATTCTCGAAA GACCTACATA GTGCATTGCC AAGACTGTGC ACGAAAAACA 
AGTGGAAACC TGGAAAATTT TGTGGTGCTA GAACAGTATA AAATGGAGGA CCTGATGCAA 
GTCTATGACC AATTTACATT AGCTCCTCCA TTACCATCCT CCTCATCTTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed