Cul7 cDNA ORF clone, Castor canadensis(American beaver)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cul7
Entrez Gene ID	109682146
Full Name	cullin 7
Gene Type	protein-coding
Organism	Castor canadensis(American beaver)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020157243.1	XP_020012832.1	cullin-7 isoform X2
XM_020157244.1	XP_020012833.1	cullin-7 isoform X3
XM_020157242.1	XP_020012831.1	cullin-7 isoform X1

Pathways from BioSystems
Gene Ontology

The following Cul7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cul7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa275836	XM_020157242.1 Latest version!	Castor canadensis cullin 7 (Cul7), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCa275837	XM_020157243.1 Latest version!	Castor canadensis cullin 7 (Cul7), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCa275838	XM_020157244.1 Latest version!	Castor canadensis cullin 7 (Cul7), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cul7 ( XM_020157242.1 ) cDNA ORF clone, Castor canadensis(American beaver) -> XP_020012831.1 Castor canadensis cullin 7 (Cul7), transcript variant X1, mRNA.

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CloneID	OCa275836
Clone ID Related Accession (Same CDS sequence)	XM_020157242.1
Accession Version	XM_020157242.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5199bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-14
Organism	Castor canadensis(American beaver)
Product	cullin-7 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017869957.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Castor canadensis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGGAG AACTCCGCTA CAGGGAATTC AGGGTGCCCC TGGGGCCTGG CTTGCATGCC 
TATCCTGAGG AGCTGATCCG CCAGCGGGTG GGCCATGACG GGCATCCTGA GTACCAGATC 
CGCTGGCTCA TCCTCAAGCG CGGAGATGAT GGGGAAGGGG GATCTGGTCA AGTGGACTGC 
AAGGCTGAGC ACATCCTGCT GTGGATGTCT GACGACGAGA TCTATGCCAA CTGCCACAAG 
ATGCTAGGCG AGGACGGCCA AGTCATCGGG CCCTCCCAGG AATCTGCAGC AGAGGCCGGC 
GCTCTGGACA AAGCCGTGCT GGGGGAGATG GAAACTGAAG TGAAGTCCCT GATTCAGAGG 
GCCCTTCGGC AGCTGGAGGA GTGTATGGGA ACTGTCCCTC CTGCTCCTCT GCTTCACACT 
GTCCATGTGC TCAGTGCCTA TGCCAGCATC GAGCCCCTCA CTGGGGTCTT CAAAGACCCA 
AGAGTCCTGG ACCTGCTCAT GCACATGCTG AGTAGTCCTG ACTACCAGAT TCGCTGGAGT 
GCAGGCCGGA TGATACAAGC CCTGTCCTCC CATGATGCTG GTGAGGGGCA GCTTGGGGAG 
GAAGGGAGAG CAGGAGAGGG GCTGGGTCGG CTCAGGGACT CACAGGACAC TGTGGCAGGA 
GCCTCTGACC TCATCAGCAC CCGGACCCAG ATCCTTCTGT CATTGAGCCA ACAAGAGGCC 
ATTGAGAAAC ACCTGGATTT TGACAGCCGC TGTGCGCTGC TAGCACTATT TGCACAGGCC 
ACTCTCTCTG AACATCCCAT GTCTTTCGAG GGTATTCAAC TGCCTCAGGT CCCAGGAAGG 
CTGCTCTTCT CCCTTGTGAA ACGCTATTTG CATGTCACCT CCCTGCTGGA TCAACTGAAT 
GACAGTGCTG GGGAGCCAGG AGCCCAGAAC AACTCTGCTC CTGAGGAGTT GAGTGTGGAG 
AGGGGCCGGT TGGAGCTGGA ATTCAGTATG ACCATGGGCA CCCTGATCTC CGAGCTGGTG 
CAGGCCATGC GCTGGGACTG GGCTTCGACC AGACCAGGGA GCTCGGCTCG GTCCCCCTGT 
TCCATCTTCC AGCCTCAGCC TGCAGATAGC AGCCCACGGC TCCCACCCTC CCAGCCCCAG 
CGTTCCTTCA AGAGGTCAAG ACGGTTTCGC CCTCGCACTG AGTTCGCAAG CGGCAACACC 
TATGCCTTAT ACGTGCGGGA CATGCTGCAG CCAGGGATGC GAGTACGAAT GCTGGATGAC 
TATGAGGAGA TCAGTAGAGG GGATGAGGGC GAGTTCCGGC AGAGCAATAG TGGAGTGCCC 
CCCGTGCAGG TGTTGTGGGA GTCGACAGGC CGCACCTACT GGGTACACTG GCACATGCTG 
GAGATCCTGG GCTTTGAGGA AGACATTGAG GATGCGGTTG AGGCTGATGA GTACCAGGGG 
GCAGTGGTCA GTGGAGCTCT GGGCAGAGCC TTGCCCTCCT GGCGCTGGAA GCCCATGACT 
GAGCTCTATG CTGTGCCTTA TGTGCTGCCT GAGGATGAGG ACACTGAGGA GAGTGAACAC 
CTGACCCAAG CCGAGTGGTG GGAGCTCCTC TTTTTCATCA AGAAGTTGGA TGGACCTGAC 
CGTCAGGGGG TTCTCCAGCT CCTACAGGAG AACCTGGATG GGGAGCACAT TTTGGACGAG 
GAGATCCTGG CTGAGCTGAC TGTGCCCATT GAGCTGGTCC AGGATCTGCT ATTGACTCTG 
CCACAGCAAC TTGATGACAG TGCCCTCAGG GACCTGCTCA ACTGCCATGT CTACAGAAAG 
TATGGGCCCG AGGCCCTGGC AGGGCAGCCA GCTCATCCAA CCCTTCTAGA AGCCCAGCAA 
GATGTGCTCC TGCCGAGAGC CCAGGGCCAG CCCCAGGAAT CAGAAGATGC AGCCAGAGTG 
GAAGCAAAAG AACCCCCCTC TCAGAGCCCC AGTGCTGCCC TGAAGTCTCT GGTGGAGGGT 
TTGGGTCCTG CTGGGAAACT CCTTGTAGAT TTGGAGCAAG CCCTTGGCTC TGGCTCAGAG 
GGGCCCCTGG AGAGCCAGGT CAAGTCCTGC CTGCTGCAGC TCCAGCGGCA GCCCCAGCCT 
TTCCTCGAAT TGATGCAGAG CCTTGACACT CCAGCCACCA ATAAGACCCT GCACCTGACT 
GTGCTGAGAA TTCTGATGAA GCTGGTAGAC TTCCCCGAGG CGCTGCTGCT CCCCTGGCAC 
GAGGCCATGG ATGCCTGCAT GGCCTGCCTG CGGTCCCCCA ACACTGACCG AGAGGTGCTC 
CAAGAACTGA TCTTCTTCCT GCACCGCCTG ACCTCAGTGA GCAGGGACTA TGCTGTGGTG 
CTCAATCAGC TGGGAGCCCG AGATGCCATC TCCAAGGCCC TGGAAAAGCA CCTGGGAAAG 
CTGGAGCTGG CTCAGGAGCT GAGGGACATG GTGTTCAAGT GTGAGAAGCA TGCCCACCTC 
TACAGGAAGC TCACCACCAA CATCCTGGGC GGCTGCATCC AGATGGTGCT GGGCCAGATC 
GAAGACCACA GACGAACCCA CCGGCCCATC AATATCCCCT TCTTTGATGT CCTCCTCAGA 
TACCTGTGCC AGGGCTCCAG CGTGGAAGTG AAGGAGGACA AGTGCTGGCA GAAGGTGGAG 
GTGTCTTCCA ACCCGCACCG GGCCTGCAGA CTGACGGACC GCAATCCCAA GACCTACTGG 
GAGTCCAAGG GTAGTGCAGG CTGTCACTCC ATCACCCTGC ACATGCGCCA GGGTGTCCAC 
ATCAGGCAGC TGACTCTGCT GGTGGCCGGG GAGGACTCAA GCTACATGCC GGCTCGGGTG 
GTGGTGTGCG GGGGCGACAG CACCAGCTCT CTTAACACAG AACTCAACTC GGTAAATGTG 
ATGCCTTGTG CCAGCCGGGT GACACTCCTG GAGAACCTGA CTCGCTTCTG GCCCATCATC 
CAGATCCGCA TAAAACGCTG CCAGCAGGGT GGCATTGACA CTCGAATTCG GGGGCTGGAA 
GTCTTGGGTC CCAAGCCCAC TTTCTGGCCA GTGTTCCGGG AGCAACTGTG TCGTCACACA 
CGCCTCTTCT ACATGGTTCG GGCGCAGGCC TGGAGCCAGG ACATAGCAGA GGACCGTAGG 
AGTCTCCTGC ACCTGAGCTC TAGGCTCAAT GGGGCTCTGC GTCAGGAGCA GAATTTTGCT 
GATCGTTTCC TCCCTGACGA CGAGGCTGCC CAAGCACTGG GCAAGACCTG CTGGGAGGCC 
CTGGTCAGCC CCCTCGTGCA GAACATCACC TGTCCCGATG AGGACGGCAC CAGCCCTCTG 
GGCTGGCTGC TGGACCAGTA CCTGGAGTGT CGGGAGGCTG CTCAGAACCC CCAGAGTCGT 
GCGGCAGCTT TCTCCTCACG GGTGCGCCGC CTCACCCACC TGCTGGTGCA CGTGGAGCCC 
TGTGAGGCAG CCCCTCCGGT GGTGGCCACC CCTCAGCCCA ACGGCAGAAC CAGAAGCCAT 
GACTGGAGGT CCTTGTCCAT CCGGGGACTT CCAAGCAGTA TCATGAGAAA CCTGACCCGC 
TGTTGGCAGG CCGTGGTGGA GGAGCAGGTG AATAACTTTC TGACCTCCTC CTGGCGGGAT 
GACGACTTTG TGCCCCGCTA CTGCGAGCAA TTTTATAACC TGCAGAAGTC AAGTTCTGAG 
CTGTTTGGGC CAAGGGCAGC CTTCTTACAG GCGCTGCAGA ATGGCTGTGC TGGTGCCTTG 
CTGAAGCTCC CTTTTCTCAG AGCTGCTCAT GTGAGTGAGC AGTTTGCTCG GCACATTGAC 
CAGCGGATCC AGGGCAGCCG GATCGGGGGA GCTCGAGGAA TGGAGATGCT GGCACAATTG 
CAACGCTGCC TGGAAACTGT CCTAAATTTC TCTGGTCTGG AGATAGCTAC CACCTTTGAG 
CATTACTACC AGCACTACAT GGCAGACCGT CTCCTGAGCT TGGGCTCCAG CTGGCTGGAG 
GGGGCCGTGC TGGAGCAGAT CGGCCTCTGT TTCCCCAACC GCCTCCCCCA GCTCATGTTG 
CAGAGCCTCA GCACCTCAGA GGAGCTGCAG CGCCAGTTCC ATGTCTATCA GCTCCAGCAG 
CTCGACCAGG AACTGCTGAA GCTGGAGGAC ACAGAGAAGA AAATTCAGGT GGACCATGAG 
GCCAGTGGCA AGGAGCATAA GAGTGAGAAG GAAGAGGATG CTGGAGCAAC AGCAGCCATG 
GCCATGGCAG AGGGAGAGGA AGAAGAGGAG AATGAGGACC TCTACTATGA AGGGGCAATG 
CCAGAAGTAT CTGTGCTCGT CCTGTCCCCA CGCTACTGGC CTGTGGCCTC CATCTGCCAC 
ACACTGAACC CCAGAACCTG CCTGCCTTCC TACCTGAGGG GCACTTTGAA TCGATATTCC 
AAGTTCTACA ACAAGAGTCA GAGCCACCCT GCCGTAACGC AAGACCTGCA GAAGGGACTG 
CAGTGGACAT GGCTGGGCCG GGCTGAGCTG CAGTTTGGGG ACCAGACCCT GCATGTGTCC 
ACAGTGCAGA TGTGGCTGCT GCTATATCTC AATGACCTGA AGGTGTTGTC TGTTGAGAGC 
CTTCGGGAGC TCTCAGGGCT TCCTCTGGAC ATGCTGAATC AAGCAATTGG GCCTCTCACC 
TCTTCAAGAG GCCCTTTGGA TCTCCAGGAG CAGAAGGATG TACCAGGAGG GGTGCTGAAG 
ATTCGAGATT GCAGCGAGGA ACCCAGGCCA AGGAGGGGCA ACGTGTGGCT CGTCCCACCT 
CAGACATACC TGAAAGCTGA GGGTGAAGAG GGCCGGAACT TGGAGAAGAG ACGGAACCTT 
CTGAACTGCC TCGTTGTCCG AATCCTCAAG GCCCATGGGG ATGAGGGCCT GCACATTGAC 
CAGCTTGTCT GTCTGGTGCT GGAGGCTTGG CAGAAGGGCC CGTGTCCTCC CAGGGGTTTA 
GTCAGCAGCC TTGGCATGGG GGCTGCATGC TGCAGCACTG ACGTCCTCTC CTGCATCCTG 
CACCTCCTGG GCAAAGGCAC ACTGAGACGC CATAATGACC GGCCCCAAAT GCTGTCCTAT 
GCAGTCCCTG TGACCGTGAT GGAGCCCCAC ACTGAGTCCT TGAACCCAGG CTCCTCAGGG 
CCCAACCCAC CCCTCACCTT CCACACCCTG CAGATTCGCT CCCGGGGTGT GCCCTATGCC 
TCCTGCACTG GCACCCAGAG TTTCTCCACC TTCCGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020012831.1
CDS	406..5604
Translation	MVGELRYREFRVPLGPGLHAYPEELIRQRVGHDGHPEYQIRWLILKRGDDGEGGSGQVDCKAEHILLWMSDDEIYANCHKMLGEDGQVIGPSQESAAEAGALDKAVLGEMETEVKSLIQRALRQLEECMGTVPPAPLLHTVHVLSAYASIEPLTGVFKDPRVLDLLMHMLSSPDYQIRWSAGRMIQALSSHDAGEGQLGEEGRAGEGLGRLRDSQDTVAGASDLISTRTQILLSLSQQEAIEKHLDFDSRCALLALFAQATLSEHPMSFEGIQLPQVPGRLLFSLVKRYLHVTSLLDQLNDSAGEPGAQNNSAPEELSVERGRLELEFSMTMGTLISELVQAMRWDWASTRPGSSARSPCSIFQPQPADSSPRLPPSQPQRSFKRSRRFRPRTEFASGNTYALYVRDMLQPGMRVRMLDDYEEISRGDEGEFRQSNSGVPPVQVLWESTGRTYWVHWHMLEILGFEEDIEDAVEADEYQGAVVSGALGRALPSWRWKPMTELYAVPYVLPEDEDTEESEHLTQAEWWELLFFIKKLDGPDRQGVLQLLQENLDGEHILDEEILAELTVPIELVQDLLLTLPQQLDDSALRDLLNCHVYRKYGPEALAGQPAHPTLLEAQQDVLLPRAQGQPQESEDAARVEAKEPPSQSPSAALKSLVEGLGPAGKLLVDLEQALGSGSEGPLESQVKSCLLQLQRQPQPFLELMQSLDTPATNKTLHLTVLRILMKLVDFPEALLLPWHEAMDACMACLRSPNTDREVLQELIFFLHRLTSVSRDYAVVLNQLGARDAISKALEKHLGKLELAQELRDMVFKCEKHAHLYRKLTTNILGGCIQMVLGQIEDHRRTHRPINIPFFDVLLRYLCQGSSVEVKEDKCWQKVEVSSNPHRACRLTDRNPKTYWESKGSAGCHSITLHMRQGVHIRQLTLLVAGEDSSYMPARVVVCGGDSTSSLNTELNSVNVMPCASRVTLLENLTRFWPIIQIRIKRCQQGGIDTRIRGLEVLGPKPTFWPVFREQLCRHTRLFYMVRAQAWSQDIAEDRRSLLHLSSRLNGALRQEQNFADRFLPDDEAAQALGKTCWEALVSPLVQNITCPDEDGTSPLGWLLDQYLECREAAQNPQSRAAAFSSRVRRLTHLLVHVEPCEAAPPVVATPQPNGRTRSHDWRSLSIRGLPSSIMRNLTRCWQAVVEEQVNNFLTSSWRDDDFVPRYCEQFYNLQKSSSELFGPRAAFLQALQNGCAGALLKLPFLRAAHVSEQFARHIDQRIQGSRIGGARGMEMLAQLQRCLETVLNFSGLEIATTFEHYYQHYMADRLLSLGSSWLEGAVLEQIGLCFPNRLPQLMLQSLSTSEELQRQFHVYQLQQLDQELLKLEDTEKKIQVDHEASGKEHKSEKEEDAGATAAMAMAEGEEEEENEDLYYEGAMPEVSVLVLSPRYWPVASICHTLNPRTCLPSYLRGTLNRYSKFYNKSQSHPAVTQDLQKGLQWTWLGRAELQFGDQTLHVSTVQMWLLLYLNDLKVLSVESLRELSGLPLDMLNQAIGPLTSSRGPLDLQEQKDVPGGVLKIRDCSEEPRPRRGNVWLVPPQTYLKAEGEEGRNLEKRRNLLNCLVVRILKAHGDEGLHIDQLVCLVLEAWQKGPCPPRGLVSSLGMGAACCSTDVLSCILHLLGKGTLRRHNDRPQMLSYAVPVTVMEPHTESLNPGSSGPNPPLTFHTLQIRSRGVPYASCTGTQSFSTFR

Target ORF information:

RefSeq Version	XM_020157242.1
Organism	Castor canadensis(American beaver)
Definition	Castor canadensis cullin 7 (Cul7), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020157242.1

ORF Insert Sequence:

ATGGTGGGAG AACTCCGCTA CAGGGAATTC AGGGTGCCCC TGGGGCCTGG CTTGCATGCC 
TATCCTGAGG AGCTGATCCG CCAGCGGGTG GGCCATGACG GGCATCCTGA GTACCAGATC 
CGCTGGCTCA TCCTCAAGCG CGGAGATGAT GGGGAAGGGG GATCTGGTCA AGTGGACTGC 
AAGGCTGAGC ACATCCTGCT GTGGATGTCT GACGACGAGA TCTATGCCAA CTGCCACAAG 
ATGCTAGGCG AGGACGGCCA AGTCATCGGG CCCTCCCAGG AATCTGCAGC AGAGGCCGGC 
GCTCTGGACA AAGCCGTGCT GGGGGAGATG GAAACTGAAG TGAAGTCCCT GATTCAGAGG 
GCCCTTCGGC AGCTGGAGGA GTGTATGGGA ACTGTCCCTC CTGCTCCTCT GCTTCACACT 
GTCCATGTGC TCAGTGCCTA TGCCAGCATC GAGCCCCTCA CTGGGGTCTT CAAAGACCCA 
AGAGTCCTGG ACCTGCTCAT GCACATGCTG AGTAGTCCTG ACTACCAGAT TCGCTGGAGT 
GCAGGCCGGA TGATACAAGC CCTGTCCTCC CATGATGCTG GTGAGGGGCA GCTTGGGGAG 
GAAGGGAGAG CAGGAGAGGG GCTGGGTCGG CTCAGGGACT CACAGGACAC TGTGGCAGGA 
GCCTCTGACC TCATCAGCAC CCGGACCCAG ATCCTTCTGT CATTGAGCCA ACAAGAGGCC 
ATTGAGAAAC ACCTGGATTT TGACAGCCGC TGTGCGCTGC TAGCACTATT TGCACAGGCC 
ACTCTCTCTG AACATCCCAT GTCTTTCGAG GGTATTCAAC TGCCTCAGGT CCCAGGAAGG 
CTGCTCTTCT CCCTTGTGAA ACGCTATTTG CATGTCACCT CCCTGCTGGA TCAACTGAAT 
GACAGTGCTG GGGAGCCAGG AGCCCAGAAC AACTCTGCTC CTGAGGAGTT GAGTGTGGAG 
AGGGGCCGGT TGGAGCTGGA ATTCAGTATG ACCATGGGCA CCCTGATCTC CGAGCTGGTG 
CAGGCCATGC GCTGGGACTG GGCTTCGACC AGACCAGGGA GCTCGGCTCG GTCCCCCTGT 
TCCATCTTCC AGCCTCAGCC TGCAGATAGC AGCCCACGGC TCCCACCCTC CCAGCCCCAG 
CGTTCCTTCA AGAGGTCAAG ACGGTTTCGC CCTCGCACTG AGTTCGCAAG CGGCAACACC 
TATGCCTTAT ACGTGCGGGA CATGCTGCAG CCAGGGATGC GAGTACGAAT GCTGGATGAC 
TATGAGGAGA TCAGTAGAGG GGATGAGGGC GAGTTCCGGC AGAGCAATAG TGGAGTGCCC 
CCCGTGCAGG TGTTGTGGGA GTCGACAGGC CGCACCTACT GGGTACACTG GCACATGCTG 
GAGATCCTGG GCTTTGAGGA AGACATTGAG GATGCGGTTG AGGCTGATGA GTACCAGGGG 
GCAGTGGTCA GTGGAGCTCT GGGCAGAGCC TTGCCCTCCT GGCGCTGGAA GCCCATGACT 
GAGCTCTATG CTGTGCCTTA TGTGCTGCCT GAGGATGAGG ACACTGAGGA GAGTGAACAC 
CTGACCCAAG CCGAGTGGTG GGAGCTCCTC TTTTTCATCA AGAAGTTGGA TGGACCTGAC 
CGTCAGGGGG TTCTCCAGCT CCTACAGGAG AACCTGGATG GGGAGCACAT TTTGGACGAG 
GAGATCCTGG CTGAGCTGAC TGTGCCCATT GAGCTGGTCC AGGATCTGCT ATTGACTCTG 
CCACAGCAAC TTGATGACAG TGCCCTCAGG GACCTGCTCA ACTGCCATGT CTACAGAAAG 
TATGGGCCCG AGGCCCTGGC AGGGCAGCCA GCTCATCCAA CCCTTCTAGA AGCCCAGCAA 
GATGTGCTCC TGCCGAGAGC CCAGGGCCAG CCCCAGGAAT CAGAAGATGC AGCCAGAGTG 
GAAGCAAAAG AACCCCCCTC TCAGAGCCCC AGTGCTGCCC TGAAGTCTCT GGTGGAGGGT 
TTGGGTCCTG CTGGGAAACT CCTTGTAGAT TTGGAGCAAG CCCTTGGCTC TGGCTCAGAG 
GGGCCCCTGG AGAGCCAGGT CAAGTCCTGC CTGCTGCAGC TCCAGCGGCA GCCCCAGCCT 
TTCCTCGAAT TGATGCAGAG CCTTGACACT CCAGCCACCA ATAAGACCCT GCACCTGACT 
GTGCTGAGAA TTCTGATGAA GCTGGTAGAC TTCCCCGAGG CGCTGCTGCT CCCCTGGCAC 
GAGGCCATGG ATGCCTGCAT GGCCTGCCTG CGGTCCCCCA ACACTGACCG AGAGGTGCTC 
CAAGAACTGA TCTTCTTCCT GCACCGCCTG ACCTCAGTGA GCAGGGACTA TGCTGTGGTG 
CTCAATCAGC TGGGAGCCCG AGATGCCATC TCCAAGGCCC TGGAAAAGCA CCTGGGAAAG 
CTGGAGCTGG CTCAGGAGCT GAGGGACATG GTGTTCAAGT GTGAGAAGCA TGCCCACCTC 
TACAGGAAGC TCACCACCAA CATCCTGGGC GGCTGCATCC AGATGGTGCT GGGCCAGATC 
GAAGACCACA GACGAACCCA CCGGCCCATC AATATCCCCT TCTTTGATGT CCTCCTCAGA 
TACCTGTGCC AGGGCTCCAG CGTGGAAGTG AAGGAGGACA AGTGCTGGCA GAAGGTGGAG 
GTGTCTTCCA ACCCGCACCG GGCCTGCAGA CTGACGGACC GCAATCCCAA GACCTACTGG 
GAGTCCAAGG GTAGTGCAGG CTGTCACTCC ATCACCCTGC ACATGCGCCA GGGTGTCCAC 
ATCAGGCAGC TGACTCTGCT GGTGGCCGGG GAGGACTCAA GCTACATGCC GGCTCGGGTG 
GTGGTGTGCG GGGGCGACAG CACCAGCTCT CTTAACACAG AACTCAACTC GGTAAATGTG 
ATGCCTTGTG CCAGCCGGGT GACACTCCTG GAGAACCTGA CTCGCTTCTG GCCCATCATC 
CAGATCCGCA TAAAACGCTG CCAGCAGGGT GGCATTGACA CTCGAATTCG GGGGCTGGAA 
GTCTTGGGTC CCAAGCCCAC TTTCTGGCCA GTGTTCCGGG AGCAACTGTG TCGTCACACA 
CGCCTCTTCT ACATGGTTCG GGCGCAGGCC TGGAGCCAGG ACATAGCAGA GGACCGTAGG 
AGTCTCCTGC ACCTGAGCTC TAGGCTCAAT GGGGCTCTGC GTCAGGAGCA GAATTTTGCT 
GATCGTTTCC TCCCTGACGA CGAGGCTGCC CAAGCACTGG GCAAGACCTG CTGGGAGGCC 
CTGGTCAGCC CCCTCGTGCA GAACATCACC TGTCCCGATG AGGACGGCAC CAGCCCTCTG 
GGCTGGCTGC TGGACCAGTA CCTGGAGTGT CGGGAGGCTG CTCAGAACCC CCAGAGTCGT 
GCGGCAGCTT TCTCCTCACG GGTGCGCCGC CTCACCCACC TGCTGGTGCA CGTGGAGCCC 
TGTGAGGCAG CCCCTCCGGT GGTGGCCACC CCTCAGCCCA ACGGCAGAAC CAGAAGCCAT 
GACTGGAGGT CCTTGTCCAT CCGGGGACTT CCAAGCAGTA TCATGAGAAA CCTGACCCGC 
TGTTGGCAGG CCGTGGTGGA GGAGCAGGTG AATAACTTTC TGACCTCCTC CTGGCGGGAT 
GACGACTTTG TGCCCCGCTA CTGCGAGCAA TTTTATAACC TGCAGAAGTC AAGTTCTGAG 
CTGTTTGGGC CAAGGGCAGC CTTCTTACAG GCGCTGCAGA ATGGCTGTGC TGGTGCCTTG 
CTGAAGCTCC CTTTTCTCAG AGCTGCTCAT GTGAGTGAGC AGTTTGCTCG GCACATTGAC 
CAGCGGATCC AGGGCAGCCG GATCGGGGGA GCTCGAGGAA TGGAGATGCT GGCACAATTG 
CAACGCTGCC TGGAAACTGT CCTAAATTTC TCTGGTCTGG AGATAGCTAC CACCTTTGAG 
CATTACTACC AGCACTACAT GGCAGACCGT CTCCTGAGCT TGGGCTCCAG CTGGCTGGAG 
GGGGCCGTGC TGGAGCAGAT CGGCCTCTGT TTCCCCAACC GCCTCCCCCA GCTCATGTTG 
CAGAGCCTCA GCACCTCAGA GGAGCTGCAG CGCCAGTTCC ATGTCTATCA GCTCCAGCAG 
CTCGACCAGG AACTGCTGAA GCTGGAGGAC ACAGAGAAGA AAATTCAGGT GGACCATGAG 
GCCAGTGGCA AGGAGCATAA GAGTGAGAAG GAAGAGGATG CTGGAGCAAC AGCAGCCATG 
GCCATGGCAG AGGGAGAGGA AGAAGAGGAG AATGAGGACC TCTACTATGA AGGGGCAATG 
CCAGAAGTAT CTGTGCTCGT CCTGTCCCCA CGCTACTGGC CTGTGGCCTC CATCTGCCAC 
ACACTGAACC CCAGAACCTG CCTGCCTTCC TACCTGAGGG GCACTTTGAA TCGATATTCC 
AAGTTCTACA ACAAGAGTCA GAGCCACCCT GCCGTAACGC AAGACCTGCA GAAGGGACTG 
CAGTGGACAT GGCTGGGCCG GGCTGAGCTG CAGTTTGGGG ACCAGACCCT GCATGTGTCC 
ACAGTGCAGA TGTGGCTGCT GCTATATCTC AATGACCTGA AGGTGTTGTC TGTTGAGAGC 
CTTCGGGAGC TCTCAGGGCT TCCTCTGGAC ATGCTGAATC AAGCAATTGG GCCTCTCACC 
TCTTCAAGAG GCCCTTTGGA TCTCCAGGAG CAGAAGGATG TACCAGGAGG GGTGCTGAAG 
ATTCGAGATT GCAGCGAGGA ACCCAGGCCA AGGAGGGGCA ACGTGTGGCT CGTCCCACCT 
CAGACATACC TGAAAGCTGA GGGTGAAGAG GGCCGGAACT TGGAGAAGAG ACGGAACCTT 
CTGAACTGCC TCGTTGTCCG AATCCTCAAG GCCCATGGGG ATGAGGGCCT GCACATTGAC 
CAGCTTGTCT GTCTGGTGCT GGAGGCTTGG CAGAAGGGCC CGTGTCCTCC CAGGGGTTTA 
GTCAGCAGCC TTGGCATGGG GGCTGCATGC TGCAGCACTG ACGTCCTCTC CTGCATCCTG 
CACCTCCTGG GCAAAGGCAC ACTGAGACGC CATAATGACC GGCCCCAAAT GCTGTCCTAT 
GCAGTCCCTG TGACCGTGAT GGAGCCCCAC ACTGAGTCCT TGAACCCAGG CTCCTCAGGG 
CCCAACCCAC CCCTCACCTT CCACACCCTG CAGATTCGCT CCCGGGGTGT GCCCTATGCC 
TCCTGCACTG GCACCCAGAG TTTCTCCACC TTCCGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cul7 ( XM_020157243.1 ) cDNA ORF clone, Castor canadensis(American beaver) -> XP_020012832.1 Castor canadensis cullin 7 (Cul7), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCa275837
Clone ID Related Accession (Same CDS sequence)	XM_020157243.1
Accession Version	XM_020157243.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-14
Organism	Castor canadensis(American beaver)
Product	cullin-7 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017869957.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Castor canadensis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGGAG AACTCCGCTA CAGGGAATTC AGGGTGCCCC TGGGGCCTGG CTTGCATGCC 
TATCCTGAGG AGCTGATCCG CCAGCGGGTG GGCCATGACG GGCATCCTGA GTACCAGATC 
CGCTGGCTCA TCCTCAAGCG CGGAGATGAT GGGGAAGGGG GATCTGGTCA AGTGGACTGC 
AAGGCTGAGC ACATCCTGCT GTGGATGTCT GACGACGAGA TCTATGCCAA CTGCCACAAG 
ATGCTAGGCG AGGACGGCCA AGTCATCGGG CCCTCCCAGG AATCTGCAGC AGAGGCCGGC 
GCTCTGGACA AAGCCGTGCT GGGGGAGATG GAAACTGAAG TGAAGTCCCT GATTCAGAGG 
GCCCTTCGGC AGCTGGAGGA GTGTATGGGA ACTGTCCCTC CTGCTCCTCT GCTTCACACT 
GTCCATGTGC TCAGTGCCTA TGCCAGCATC GAGCCCCTCA CTGGGGTCTT CAAAGACCCA 
AGAGTCCTGG ACCTGCTCAT GCACATGCTG AGTAGTCCTG ACTACCAGAT TCGCTGGAGT 
GCAGGCCGGA TGATACAAGC CCTGTCCTCC CATGATGCTG GCACCCGGAC CCAGATCCTT 
CTGTCATTGA GCCAACAAGA GGCCATTGAG AAACACCTGG ATTTTGACAG CCGCTGTGCG 
CTGCTAGCAC TATTTGCACA GGCCACTCTC TCTGAACATC CCATGTCTTT CGAGGGTATT 
CAACTGCCTC AGGTCCCAGG AAGGCTGCTC TTCTCCCTTG TGAAACGCTA TTTGCATGTC 
ACCTCCCTGC TGGATCAACT GAATGACAGT GCTGGGGAGC CAGGAGCCCA GAACAACTCT 
GCTCCTGAGG AGTTGAGTGT GGAGAGGGGC CGGTTGGAGC TGGAATTCAG TATGACCATG 
GGCACCCTGA TCTCCGAGCT GGTGCAGGCC ATGCGCTGGG ACTGGGCTTC GACCAGACCA 
GGGAGCTCGG CTCGGTCCCC CTGTTCCATC TTCCAGCCTC AGCCTGCAGA TAGCAGCCCA 
CGGCTCCCAC CCTCCCAGCC CCAGCGTTCC TTCAAGAGGT CAAGACGGTT TCGCCCTCGC 
ACTGAGTTCG CAAGCGGCAA CACCTATGCC TTATACGTGC GGGACATGCT GCAGCCAGGG 
ATGCGAGTAC GAATGCTGGA TGACTATGAG GAGATCAGTA GAGGGGATGA GGGCGAGTTC 
CGGCAGAGCA ATAGTGGAGT GCCCCCCGTG CAGGTGTTGT GGGAGTCGAC AGGCCGCACC 
TACTGGGTAC ACTGGCACAT GCTGGAGATC CTGGGCTTTG AGGAAGACAT TGAGGATGCG 
GTTGAGGCTG ATGAGTACCA GGGGGCAGTG GTCAGTGGAG CTCTGGGCAG AGCCTTGCCC 
TCCTGGCGCT GGAAGCCCAT GACTGAGCTC TATGCTGTGC CTTATGTGCT GCCTGAGGAT 
GAGGACACTG AGGAGAGTGA ACACCTGACC CAAGCCGAGT GGTGGGAGCT CCTCTTTTTC 
ATCAAGAAGT TGGATGGACC TGACCGTCAG GGGGTTCTCC AGCTCCTACA GGAGAACCTG 
GATGGGGAGC ACATTTTGGA CGAGGAGATC CTGGCTGAGC TGACTGTGCC CATTGAGCTG 
GTCCAGGATC TGCTATTGAC TCTGCCACAG CAACTTGATG ACAGTGCCCT CAGGGACCTG 
CTCAACTGCC ATGTCTACAG AAAGTATGGG CCCGAGGCCC TGGCAGGGCA GCCAGCTCAT 
CCAACCCTTC TAGAAGCCCA GCAAGATGTG CTCCTGCCGA GAGCCCAGGG CCAGCCCCAG 
GAATCAGAAG ATGCAGCCAG AGTGGAAGCA AAAGAACCCC CCTCTCAGAG CCCCAGTGCT 
GCCCTGAAGT CTCTGGTGGA GGGTTTGGGT CCTGCTGGGA AACTCCTTGT AGATTTGGAG 
CAAGCCCTTG GCTCTGGCTC AGAGGGGCCC CTGGAGAGCC AGGTCAAGTC CTGCCTGCTG 
CAGCTCCAGC GGCAGCCCCA GCCTTTCCTC GAATTGATGC AGAGCCTTGA CACTCCAGCC 
ACCAATAAGA CCCTGCACCT GACTGTGCTG AGAATTCTGA TGAAGCTGGT AGACTTCCCC 
GAGGCGCTGC TGCTCCCCTG GCACGAGGCC ATGGATGCCT GCATGGCCTG CCTGCGGTCC 
CCCAACACTG ACCGAGAGGT GCTCCAAGAA CTGATCTTCT TCCTGCACCG CCTGACCTCA 
GTGAGCAGGG ACTATGCTGT GGTGCTCAAT CAGCTGGGAG CCCGAGATGC CATCTCCAAG 
GCCCTGGAAA AGCACCTGGG AAAGCTGGAG CTGGCTCAGG AGCTGAGGGA CATGGTGTTC 
AAGTGTGAGA AGCATGCCCA CCTCTACAGG AAGCTCACCA CCAACATCCT GGGCGGCTGC 
ATCCAGATGG TGCTGGGCCA GATCGAAGAC CACAGACGAA CCCACCGGCC CATCAATATC 
CCCTTCTTTG ATGTCCTCCT CAGATACCTG TGCCAGGGCT CCAGCGTGGA AGTGAAGGAG 
GACAAGTGCT GGCAGAAGGT GGAGGTGTCT TCCAACCCGC ACCGGGCCTG CAGACTGACG 
GACCGCAATC CCAAGACCTA CTGGGAGTCC AAGGGTAGTG CAGGCTGTCA CTCCATCACC 
CTGCACATGC GCCAGGGTGT CCACATCAGG CAGCTGACTC TGCTGGTGGC CGGGGAGGAC 
TCAAGCTACA TGCCGGCTCG GGTGGTGGTG TGCGGGGGCG ACAGCACCAG CTCTCTTAAC 
ACAGAACTCA ACTCGGTAAA TGTGATGCCT TGTGCCAGCC GGGTGACACT CCTGGAGAAC 
CTGACTCGCT TCTGGCCCAT CATCCAGATC CGCATAAAAC GCTGCCAGCA GGGTGGCATT 
GACACTCGAA TTCGGGGGCT GGAAGTCTTG GGTCCCAAGC CCACTTTCTG GCCAGTGTTC 
CGGGAGCAAC TGTGTCGTCA CACACGCCTC TTCTACATGG TTCGGGCGCA GGCCTGGAGC 
CAGGACATAG CAGAGGACCG TAGGAGTCTC CTGCACCTGA GCTCTAGGCT CAATGGGGCT 
CTGCGTCAGG AGCAGAATTT TGCTGATCGT TTCCTCCCTG ACGACGAGGC TGCCCAAGCA 
CTGGGCAAGA CCTGCTGGGA GGCCCTGGTC AGCCCCCTCG TGCAGAACAT CACCTGTCCC 
GATGAGGACG GCACCAGCCC TCTGGGCTGG CTGCTGGACC AGTACCTGGA GTGTCGGGAG 
GCTGCTCAGA ACCCCCAGAG TCGTGCGGCA GCTTTCTCCT CACGGGTGCG CCGCCTCACC 
CACCTGCTGG TGCACGTGGA GCCCTGTGAG GCAGCCCCTC CGGTGGTGGC CACCCCTCAG 
CCCAACGGCA GAACCAGAAG CCATGACTGG AGGTCCTTGT CCATCCGGGG ACTTCCAAGC 
AGTATCATGA GAAACCTGAC CCGCTGTTGG CAGGCCGTGG TGGAGGAGCA GGTGAATAAC 
TTTCTGACCT CCTCCTGGCG GGATGACGAC TTTGTGCCCC GCTACTGCGA GCAATTTTAT 
AACCTGCAGA AGTCAAGTTC TGAGCTGTTT GGGCCAAGGG CAGCCTTCTT ACAGGCGCTG 
CAGAATGGCT GTGCTGGTGC CTTGCTGAAG CTCCCTTTTC TCAGAGCTGC TCATGTGAGT 
GAGCAGTTTG CTCGGCACAT TGACCAGCGG ATCCAGGGCA GCCGGATCGG GGGAGCTCGA 
GGAATGGAGA TGCTGGCACA ATTGCAACGC TGCCTGGAAA CTGTCCTAAA TTTCTCTGGT 
CTGGAGATAG CTACCACCTT TGAGCATTAC TACCAGCACT ACATGGCAGA CCGTCTCCTG 
AGCTTGGGCT CCAGCTGGCT GGAGGGGGCC GTGCTGGAGC AGATCGGCCT CTGTTTCCCC 
AACCGCCTCC CCCAGCTCAT GTTGCAGAGC CTCAGCACCT CAGAGGAGCT GCAGCGCCAG 
TTCCATGTCT ATCAGCTCCA GCAGCTCGAC CAGGAACTGC TGAAGCTGGA GGACACAGAG 
AAGAAAATTC AGGTGGACCA TGAGGCCAGT GGCAAGGAGC ATAAGAGTGA GAAGGAAGAG 
GATGCTGGAG CAACAGCAGC CATGGCCATG GCAGAGGGAG AGGAAGAAGA GGAGAATGAG 
GACCTCTACT ATGAAGGGGC AATGCCAGAA GTATCTGTGC TCGTCCTGTC CCCACGCTAC 
TGGCCTGTGG CCTCCATCTG CCACACACTG AACCCCAGAA CCTGCCTGCC TTCCTACCTG 
AGGGGCACTT TGAATCGATA TTCCAAGTTC TACAACAAGA GTCAGAGCCA CCCTGCCGTA 
ACGCAAGACC TGCAGAAGGG ACTGCAGTGG ACATGGCTGG GCCGGGCTGA GCTGCAGTTT 
GGGGACCAGA CCCTGCATGT GTCCACAGTG CAGATGTGGC TGCTGCTATA TCTCAATGAC 
CTGAAGGTGT TGTCTGTTGA GAGCCTTCGG GAGCTCTCAG GGCTTCCTCT GGACATGCTG 
AATCAAGCAA TTGGGCCTCT CACCTCTTCA AGAGGCCCTT TGGATCTCCA GGAGCAGAAG 
GATGTACCAG GAGGGGTGCT GAAGATTCGA GATTGCAGCG AGGAACCCAG GCCAAGGAGG 
GGCAACGTGT GGCTCGTCCC ACCTCAGACA TACCTGAAAG CTGAGGGTGA AGAGGGCCGG 
AACTTGGAGA AGAGACGGAA CCTTCTGAAC TGCCTCGTTG TCCGAATCCT CAAGGCCCAT 
GGGGATGAGG GCCTGCACAT TGACCAGCTT GTCTGTCTGG TGCTGGAGGC TTGGCAGAAG 
GGCCCGTGTC CTCCCAGGGG TTTAGTCAGC AGCCTTGGCA TGGGGGCTGC ATGCTGCAGC 
ACTGACGTCC TCTCCTGCAT CCTGCACCTC CTGGGCAAAG GCACACTGAG ACGCCATAAT 
GACCGGCCCC AAATGCTGTC CTATGCAGTC CCTGTGACCG TGATGGAGCC CCACACTGAG 
TCCTTGAACC CAGGCTCCTC AGGGCCCAAC CCACCCCTCA CCTTCCACAC CCTGCAGATT 
CGCTCCCGGG GTGTGCCCTA TGCCTCCTGC ACTGGCACCC AGAGTTTCTC CACCTTCCGA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020012832.1
CDS	406..5508
Translation	MVGELRYREFRVPLGPGLHAYPEELIRQRVGHDGHPEYQIRWLILKRGDDGEGGSGQVDCKAEHILLWMSDDEIYANCHKMLGEDGQVIGPSQESAAEAGALDKAVLGEMETEVKSLIQRALRQLEECMGTVPPAPLLHTVHVLSAYASIEPLTGVFKDPRVLDLLMHMLSSPDYQIRWSAGRMIQALSSHDAGTRTQILLSLSQQEAIEKHLDFDSRCALLALFAQATLSEHPMSFEGIQLPQVPGRLLFSLVKRYLHVTSLLDQLNDSAGEPGAQNNSAPEELSVERGRLELEFSMTMGTLISELVQAMRWDWASTRPGSSARSPCSIFQPQPADSSPRLPPSQPQRSFKRSRRFRPRTEFASGNTYALYVRDMLQPGMRVRMLDDYEEISRGDEGEFRQSNSGVPPVQVLWESTGRTYWVHWHMLEILGFEEDIEDAVEADEYQGAVVSGALGRALPSWRWKPMTELYAVPYVLPEDEDTEESEHLTQAEWWELLFFIKKLDGPDRQGVLQLLQENLDGEHILDEEILAELTVPIELVQDLLLTLPQQLDDSALRDLLNCHVYRKYGPEALAGQPAHPTLLEAQQDVLLPRAQGQPQESEDAARVEAKEPPSQSPSAALKSLVEGLGPAGKLLVDLEQALGSGSEGPLESQVKSCLLQLQRQPQPFLELMQSLDTPATNKTLHLTVLRILMKLVDFPEALLLPWHEAMDACMACLRSPNTDREVLQELIFFLHRLTSVSRDYAVVLNQLGARDAISKALEKHLGKLELAQELRDMVFKCEKHAHLYRKLTTNILGGCIQMVLGQIEDHRRTHRPINIPFFDVLLRYLCQGSSVEVKEDKCWQKVEVSSNPHRACRLTDRNPKTYWESKGSAGCHSITLHMRQGVHIRQLTLLVAGEDSSYMPARVVVCGGDSTSSLNTELNSVNVMPCASRVTLLENLTRFWPIIQIRIKRCQQGGIDTRIRGLEVLGPKPTFWPVFREQLCRHTRLFYMVRAQAWSQDIAEDRRSLLHLSSRLNGALRQEQNFADRFLPDDEAAQALGKTCWEALVSPLVQNITCPDEDGTSPLGWLLDQYLECREAAQNPQSRAAAFSSRVRRLTHLLVHVEPCEAAPPVVATPQPNGRTRSHDWRSLSIRGLPSSIMRNLTRCWQAVVEEQVNNFLTSSWRDDDFVPRYCEQFYNLQKSSSELFGPRAAFLQALQNGCAGALLKLPFLRAAHVSEQFARHIDQRIQGSRIGGARGMEMLAQLQRCLETVLNFSGLEIATTFEHYYQHYMADRLLSLGSSWLEGAVLEQIGLCFPNRLPQLMLQSLSTSEELQRQFHVYQLQQLDQELLKLEDTEKKIQVDHEASGKEHKSEKEEDAGATAAMAMAEGEEEEENEDLYYEGAMPEVSVLVLSPRYWPVASICHTLNPRTCLPSYLRGTLNRYSKFYNKSQSHPAVTQDLQKGLQWTWLGRAELQFGDQTLHVSTVQMWLLLYLNDLKVLSVESLRELSGLPLDMLNQAIGPLTSSRGPLDLQEQKDVPGGVLKIRDCSEEPRPRRGNVWLVPPQTYLKAEGEEGRNLEKRRNLLNCLVVRILKAHGDEGLHIDQLVCLVLEAWQKGPCPPRGLVSSLGMGAACCSTDVLSCILHLLGKGTLRRHNDRPQMLSYAVPVTVMEPHTESLNPGSSGPNPPLTFHTLQIRSRGVPYASCTGTQSFSTFR

Target ORF information:

RefSeq Version	XM_020157243.1
Organism	Castor canadensis(American beaver)
Definition	Castor canadensis cullin 7 (Cul7), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020157243.1

ORF Insert Sequence:

ATGGTGGGAG AACTCCGCTA CAGGGAATTC AGGGTGCCCC TGGGGCCTGG CTTGCATGCC 
TATCCTGAGG AGCTGATCCG CCAGCGGGTG GGCCATGACG GGCATCCTGA GTACCAGATC 
CGCTGGCTCA TCCTCAAGCG CGGAGATGAT GGGGAAGGGG GATCTGGTCA AGTGGACTGC 
AAGGCTGAGC ACATCCTGCT GTGGATGTCT GACGACGAGA TCTATGCCAA CTGCCACAAG 
ATGCTAGGCG AGGACGGCCA AGTCATCGGG CCCTCCCAGG AATCTGCAGC AGAGGCCGGC 
GCTCTGGACA AAGCCGTGCT GGGGGAGATG GAAACTGAAG TGAAGTCCCT GATTCAGAGG 
GCCCTTCGGC AGCTGGAGGA GTGTATGGGA ACTGTCCCTC CTGCTCCTCT GCTTCACACT 
GTCCATGTGC TCAGTGCCTA TGCCAGCATC GAGCCCCTCA CTGGGGTCTT CAAAGACCCA 
AGAGTCCTGG ACCTGCTCAT GCACATGCTG AGTAGTCCTG ACTACCAGAT TCGCTGGAGT 
GCAGGCCGGA TGATACAAGC CCTGTCCTCC CATGATGCTG GCACCCGGAC CCAGATCCTT 
CTGTCATTGA GCCAACAAGA GGCCATTGAG AAACACCTGG ATTTTGACAG CCGCTGTGCG 
CTGCTAGCAC TATTTGCACA GGCCACTCTC TCTGAACATC CCATGTCTTT CGAGGGTATT 
CAACTGCCTC AGGTCCCAGG AAGGCTGCTC TTCTCCCTTG TGAAACGCTA TTTGCATGTC 
ACCTCCCTGC TGGATCAACT GAATGACAGT GCTGGGGAGC CAGGAGCCCA GAACAACTCT 
GCTCCTGAGG AGTTGAGTGT GGAGAGGGGC CGGTTGGAGC TGGAATTCAG TATGACCATG 
GGCACCCTGA TCTCCGAGCT GGTGCAGGCC ATGCGCTGGG ACTGGGCTTC GACCAGACCA 
GGGAGCTCGG CTCGGTCCCC CTGTTCCATC TTCCAGCCTC AGCCTGCAGA TAGCAGCCCA 
CGGCTCCCAC CCTCCCAGCC CCAGCGTTCC TTCAAGAGGT CAAGACGGTT TCGCCCTCGC 
ACTGAGTTCG CAAGCGGCAA CACCTATGCC TTATACGTGC GGGACATGCT GCAGCCAGGG 
ATGCGAGTAC GAATGCTGGA TGACTATGAG GAGATCAGTA GAGGGGATGA GGGCGAGTTC 
CGGCAGAGCA ATAGTGGAGT GCCCCCCGTG CAGGTGTTGT GGGAGTCGAC AGGCCGCACC 
TACTGGGTAC ACTGGCACAT GCTGGAGATC CTGGGCTTTG AGGAAGACAT TGAGGATGCG 
GTTGAGGCTG ATGAGTACCA GGGGGCAGTG GTCAGTGGAG CTCTGGGCAG AGCCTTGCCC 
TCCTGGCGCT GGAAGCCCAT GACTGAGCTC TATGCTGTGC CTTATGTGCT GCCTGAGGAT 
GAGGACACTG AGGAGAGTGA ACACCTGACC CAAGCCGAGT GGTGGGAGCT CCTCTTTTTC 
ATCAAGAAGT TGGATGGACC TGACCGTCAG GGGGTTCTCC AGCTCCTACA GGAGAACCTG 
GATGGGGAGC ACATTTTGGA CGAGGAGATC CTGGCTGAGC TGACTGTGCC CATTGAGCTG 
GTCCAGGATC TGCTATTGAC TCTGCCACAG CAACTTGATG ACAGTGCCCT CAGGGACCTG 
CTCAACTGCC ATGTCTACAG AAAGTATGGG CCCGAGGCCC TGGCAGGGCA GCCAGCTCAT 
CCAACCCTTC TAGAAGCCCA GCAAGATGTG CTCCTGCCGA GAGCCCAGGG CCAGCCCCAG 
GAATCAGAAG ATGCAGCCAG AGTGGAAGCA AAAGAACCCC CCTCTCAGAG CCCCAGTGCT 
GCCCTGAAGT CTCTGGTGGA GGGTTTGGGT CCTGCTGGGA AACTCCTTGT AGATTTGGAG 
CAAGCCCTTG GCTCTGGCTC AGAGGGGCCC CTGGAGAGCC AGGTCAAGTC CTGCCTGCTG 
CAGCTCCAGC GGCAGCCCCA GCCTTTCCTC GAATTGATGC AGAGCCTTGA CACTCCAGCC 
ACCAATAAGA CCCTGCACCT GACTGTGCTG AGAATTCTGA TGAAGCTGGT AGACTTCCCC 
GAGGCGCTGC TGCTCCCCTG GCACGAGGCC ATGGATGCCT GCATGGCCTG CCTGCGGTCC 
CCCAACACTG ACCGAGAGGT GCTCCAAGAA CTGATCTTCT TCCTGCACCG CCTGACCTCA 
GTGAGCAGGG ACTATGCTGT GGTGCTCAAT CAGCTGGGAG CCCGAGATGC CATCTCCAAG 
GCCCTGGAAA AGCACCTGGG AAAGCTGGAG CTGGCTCAGG AGCTGAGGGA CATGGTGTTC 
AAGTGTGAGA AGCATGCCCA CCTCTACAGG AAGCTCACCA CCAACATCCT GGGCGGCTGC 
ATCCAGATGG TGCTGGGCCA GATCGAAGAC CACAGACGAA CCCACCGGCC CATCAATATC 
CCCTTCTTTG ATGTCCTCCT CAGATACCTG TGCCAGGGCT CCAGCGTGGA AGTGAAGGAG 
GACAAGTGCT GGCAGAAGGT GGAGGTGTCT TCCAACCCGC ACCGGGCCTG CAGACTGACG 
GACCGCAATC CCAAGACCTA CTGGGAGTCC AAGGGTAGTG CAGGCTGTCA CTCCATCACC 
CTGCACATGC GCCAGGGTGT CCACATCAGG CAGCTGACTC TGCTGGTGGC CGGGGAGGAC 
TCAAGCTACA TGCCGGCTCG GGTGGTGGTG TGCGGGGGCG ACAGCACCAG CTCTCTTAAC 
ACAGAACTCA ACTCGGTAAA TGTGATGCCT TGTGCCAGCC GGGTGACACT CCTGGAGAAC 
CTGACTCGCT TCTGGCCCAT CATCCAGATC CGCATAAAAC GCTGCCAGCA GGGTGGCATT 
GACACTCGAA TTCGGGGGCT GGAAGTCTTG GGTCCCAAGC CCACTTTCTG GCCAGTGTTC 
CGGGAGCAAC TGTGTCGTCA CACACGCCTC TTCTACATGG TTCGGGCGCA GGCCTGGAGC 
CAGGACATAG CAGAGGACCG TAGGAGTCTC CTGCACCTGA GCTCTAGGCT CAATGGGGCT 
CTGCGTCAGG AGCAGAATTT TGCTGATCGT TTCCTCCCTG ACGACGAGGC TGCCCAAGCA 
CTGGGCAAGA CCTGCTGGGA GGCCCTGGTC AGCCCCCTCG TGCAGAACAT CACCTGTCCC 
GATGAGGACG GCACCAGCCC TCTGGGCTGG CTGCTGGACC AGTACCTGGA GTGTCGGGAG 
GCTGCTCAGA ACCCCCAGAG TCGTGCGGCA GCTTTCTCCT CACGGGTGCG CCGCCTCACC 
CACCTGCTGG TGCACGTGGA GCCCTGTGAG GCAGCCCCTC CGGTGGTGGC CACCCCTCAG 
CCCAACGGCA GAACCAGAAG CCATGACTGG AGGTCCTTGT CCATCCGGGG ACTTCCAAGC 
AGTATCATGA GAAACCTGAC CCGCTGTTGG CAGGCCGTGG TGGAGGAGCA GGTGAATAAC 
TTTCTGACCT CCTCCTGGCG GGATGACGAC TTTGTGCCCC GCTACTGCGA GCAATTTTAT 
AACCTGCAGA AGTCAAGTTC TGAGCTGTTT GGGCCAAGGG CAGCCTTCTT ACAGGCGCTG 
CAGAATGGCT GTGCTGGTGC CTTGCTGAAG CTCCCTTTTC TCAGAGCTGC TCATGTGAGT 
GAGCAGTTTG CTCGGCACAT TGACCAGCGG ATCCAGGGCA GCCGGATCGG GGGAGCTCGA 
GGAATGGAGA TGCTGGCACA ATTGCAACGC TGCCTGGAAA CTGTCCTAAA TTTCTCTGGT 
CTGGAGATAG CTACCACCTT TGAGCATTAC TACCAGCACT ACATGGCAGA CCGTCTCCTG 
AGCTTGGGCT CCAGCTGGCT GGAGGGGGCC GTGCTGGAGC AGATCGGCCT CTGTTTCCCC 
AACCGCCTCC CCCAGCTCAT GTTGCAGAGC CTCAGCACCT CAGAGGAGCT GCAGCGCCAG 
TTCCATGTCT ATCAGCTCCA GCAGCTCGAC CAGGAACTGC TGAAGCTGGA GGACACAGAG 
AAGAAAATTC AGGTGGACCA TGAGGCCAGT GGCAAGGAGC ATAAGAGTGA GAAGGAAGAG 
GATGCTGGAG CAACAGCAGC CATGGCCATG GCAGAGGGAG AGGAAGAAGA GGAGAATGAG 
GACCTCTACT ATGAAGGGGC AATGCCAGAA GTATCTGTGC TCGTCCTGTC CCCACGCTAC 
TGGCCTGTGG CCTCCATCTG CCACACACTG AACCCCAGAA CCTGCCTGCC TTCCTACCTG 
AGGGGCACTT TGAATCGATA TTCCAAGTTC TACAACAAGA GTCAGAGCCA CCCTGCCGTA 
ACGCAAGACC TGCAGAAGGG ACTGCAGTGG ACATGGCTGG GCCGGGCTGA GCTGCAGTTT 
GGGGACCAGA CCCTGCATGT GTCCACAGTG CAGATGTGGC TGCTGCTATA TCTCAATGAC 
CTGAAGGTGT TGTCTGTTGA GAGCCTTCGG GAGCTCTCAG GGCTTCCTCT GGACATGCTG 
AATCAAGCAA TTGGGCCTCT CACCTCTTCA AGAGGCCCTT TGGATCTCCA GGAGCAGAAG 
GATGTACCAG GAGGGGTGCT GAAGATTCGA GATTGCAGCG AGGAACCCAG GCCAAGGAGG 
GGCAACGTGT GGCTCGTCCC ACCTCAGACA TACCTGAAAG CTGAGGGTGA AGAGGGCCGG 
AACTTGGAGA AGAGACGGAA CCTTCTGAAC TGCCTCGTTG TCCGAATCCT CAAGGCCCAT 
GGGGATGAGG GCCTGCACAT TGACCAGCTT GTCTGTCTGG TGCTGGAGGC TTGGCAGAAG 
GGCCCGTGTC CTCCCAGGGG TTTAGTCAGC AGCCTTGGCA TGGGGGCTGC ATGCTGCAGC 
ACTGACGTCC TCTCCTGCAT CCTGCACCTC CTGGGCAAAG GCACACTGAG ACGCCATAAT 
GACCGGCCCC AAATGCTGTC CTATGCAGTC CCTGTGACCG TGATGGAGCC CCACACTGAG 
TCCTTGAACC CAGGCTCCTC AGGGCCCAAC CCACCCCTCA CCTTCCACAC CCTGCAGATT 
CGCTCCCGGG GTGTGCCCTA TGCCTCCTGC ACTGGCACCC AGAGTTTCTC CACCTTCCGA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cul7 ( XM_020157244.1 ) cDNA ORF clone, Castor canadensis(American beaver) -> XP_020012833.1 Castor canadensis cullin 7 (Cul7), transcript variant X3, mRNA.

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CloneID	OCa275838
Clone ID Related Accession (Same CDS sequence)	XM_020157244.1
Accession Version	XM_020157244.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5445bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-14
Organism	Castor canadensis(American beaver)
Product	cullin-7 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017869957.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Castor canadensis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCGTG GCTCTGGGTG GGCGGCAGAC ACCTCACACA CACACCCAAA AGCTGGGCGC 
ACGGTGCCGC CCGCTGGGAC CAGATCCCGC CCTGCCCCAG CGGAGCTGAC GCGCAAGGGC 
GCTGAGGACA GCGGCCCGGC CCCTAGGTCC CCGCGCGCCA CACCCCTTGC CCGCCTGTCT 
GTCCAGCAGA GACACGCCCC CTCCCGCCTA TACGCCTCGA CCCCCTCGGG GGGCCCAGGT 
GCCAGGATGG TGGGAGAACT CCGCTACAGG GAATTCAGGG TGCCCCTGGG GCCTGGCTTG 
CATGCCTATC CTGAGGAGCT GATCCGCCAG CGGGTGGGCC ATGACGGGCA TCCTGAGTAC 
CAGATCCGCT GGCTCATCCT CAAGCGCGGA GATGATGGGG AAGGGGGATC TGGTCAAGTG 
GACTGCAAGG CTGAGCACAT CCTGCTGTGG ATGTCTGACG ACGAGATCTA TGCCAACTGC 
CACAAGATGC TAGGCGAGGA CGGCCAAGTC ATCGGGCCCT CCCAGGAATC TGCAGCAGAG 
GCCGGCGCTC TGGACAAAGC CGTGCTGGGG GAGATGGAAA CTGAAGTGAA GTCCCTGATT 
CAGAGGGCCC TTCGGCAGCT GGAGGAGTGT ATGGGAACTG TCCCTCCTGC TCCTCTGCTT 
CACACTGTCC ATGTGCTCAG TGCCTATGCC AGCATCGAGC CCCTCACTGG GGTCTTCAAA 
GACCCAAGAG TCCTGGACCT GCTCATGCAC ATGCTGAGTA GTCCTGACTA CCAGATTCGC 
TGGAGTGCAG GCCGGATGAT ACAAGCCCTG TCCTCCCATG ATGCTGGTGA GGGGCAGCTT 
GGGGAGGAAG GGAGAGCAGG AGAGGGGCTG GGTCGGCTCA GGGACTCACA GGACACTGTG 
GCAGGAGCCT CTGACCTCAT CAGCACCCGG ACCCAGATCC TTCTGTCATT GAGCCAACAA 
GAGGCCATTG AGAAACACCT GGATTTTGAC AGCCGCTGTG CGCTGCTAGC ACTATTTGCA 
CAGGCCACTC TCTCTGAACA TCCCATGTCT TTCGAGGGTA TTCAACTGCC TCAGGTCCCA 
GGAAGGCTGC TCTTCTCCCT TGTGAAACGC TATTTGCATG TCACCTCCCT GCTGGATCAA 
CTGAATGACA GTGCTGGGGA GCCAGGAGCC CAGAACAACT CTGCTCCTGA GGAGTTGAGT 
GTGGAGAGGG GCCGGTTGGA GCTGGAATTC AGTATGACCA TGGGCACCCT GATCTCCGAG 
CTGGTGCAGG CCATGCGCTG GGACTGGGCT TCGACCAGAC CAGGGAGCTC GGCTCGGTCC 
CCCTGTTCCA TCTTCCAGCC TCAGCCTGCA GATAGCAGCC CACGGCTCCC ACCCTCCCAG 
CCCCAGCGTT CCTTCAAGAG GTCAAGACGG TTTCGCCCTC GCACTGAGTT CGCAAGCGGC 
AACACCTATG CCTTATACGT GCGGGACATG CTGCAGCCAG GGATGCGAGT ACGAATGCTG 
GATGACTATG AGGAGATCAG TAGAGGGGAT GAGGGCGAGT TCCGGCAGAG CAATAGTGGA 
GTGCCCCCCG TGCAGGTGTT GTGGGAGTCG ACAGGCCGCA CCTACTGGGT ACACTGGCAC 
ATGCTGGAGA TCCTGGGCTT TGAGGAAGAC ATTGAGGATG CGGTTGAGGC TGATGAGTAC 
CAGGGGGCAG TGGTCAGTGG AGCTCTGGGC AGAGCCTTGC CCTCCTGGCG CTGGAAGCCC 
ATGACTGAGC TCTATGCTGT GCCTTATGTG CTGCCTGAGG ATGAGGACAC TGAGGAGAGT 
GAACACCTGA CCCAAGCCGA GTGGTGGGAG CTCCTCTTTT TCATCAAGAA GTTGGATGGA 
CCTGACCGTC AGGGGGTTCT CCAGCTCCTA CAGGAGAACC TGGATGGGGA GCACATTTTG 
GACGAGGAGA TCCTGGCTGA GCTGACTGTG CCCATTGAGC TGGTCCAGGA TCTGCTATTG 
ACTCTGCCAC AGCAACTTGA TGACAGTGCC CTCAGGGACC TGCTCAACTG CCATGTCTAC 
AGAAAGTATG GGCCCGAGGC CCTGGCAGGG CAGCCAGCTC ATCCAACCCT TCTAGAAGCC 
CAGCAAGATG TGCTCCTGCC GAGAGCCCAG GGCCAGCCCC AGGAATCAGA AGATGCAGCC 
AGAGTGGAAG CAAAAGAACC CCCCTCTCAG AGCCCCAGTG CTGCCCTGAA GTCTCTGGTG 
GAGGGTTTGG GTCCTGCTGG GAAACTCCTT GTAGATTTGG AGCAAGCCCT TGGCTCTGGC 
TCAGAGGGGC CCCTGGAGAG CCAGGTCAAG TCCTGCCTGC TGCAGCTCCA GCGGCAGCCC 
CAGCCTTTCC TCGAATTGAT GCAGAGCCTT GACACTCCAG CCACCAATAA GACCCTGCAC 
CTGACTGTGC TGAGAATTCT GATGAAGCTG GTAGACTTCC CCGAGGCGCT GCTGCTCCCC 
TGGCACGAGG CCATGGATGC CTGCATGGCC TGCCTGCGGT CCCCCAACAC TGACCGAGAG 
GTGCTCCAAG AACTGATCTT CTTCCTGCAC CGCCTGACCT CAGTGAGCAG GGACTATGCT 
GTGGTGCTCA ATCAGCTGGG AGCCCGAGAT GCCATCTCCA AGGCCCTGGA AAAGCACCTG 
GGAAAGCTGG AGCTGGCTCA GGAGCTGAGG GACATGGTGT TCAAGTGTGA GAAGCATGCC 
CACCTCTACA GGAAGCTCAC CACCAACATC CTGGGCGGCT GCATCCAGAT GGTGCTGGGC 
CAGATCGAAG ACCACAGACG AACCCACCGG CCCATCAATA TCCCCTTCTT TGATGTCCTC 
CTCAGATACC TGTGCCAGGG CTCCAGCGTG GAAGTGAAGG AGGACAAGTG CTGGCAGAAG 
GTGGAGGTGT CTTCCAACCC GCACCGGGCC TGCAGACTGA CGGACCGCAA TCCCAAGACC 
TACTGGGAGT CCAAGGGTAG TGCAGGCTGT CACTCCATCA CCCTGCACAT GCGCCAGGGT 
GTCCACATCA GGCAGCTGAC TCTGCTGGTG GCCGGGGAGG ACTCAAGCTA CATGCCGGCT 
CGGGTGGTGG TGTGCGGGGG CGACAGCACC AGCTCTCTTA ACACAGAACT CAACTCGGTA 
AATGTGATGC CTTGTGCCAG CCGGGTGACA CTCCTGGAGA ACCTGACTCG CTTCTGGCCC 
ATCATCCAGA TCCGCATAAA ACGCTGCCAG CAGGGTGGCA TTGACACTCG AATTCGGGGG 
CTGGAAGTCT TGGGTCCCAA GCCCACTTTC TGGCCAGTGT TCCGGGAGCA ACTGTGTCGT 
CACACACGCC TCTTCTACAT GGTTCGGGCG CAGGCCTGGA GCCAGGACAT AGCAGAGGAC 
CGTAGGAGTC TCCTGCACCT GAGCTCTAGG CTCAATGGGG CTCTGCGTCA GGAGCAGAAT 
TTTGCTGATC GTTTCCTCCC TGACGACGAG GCTGCCCAAG CACTGGGCAA GACCTGCTGG 
GAGGCCCTGG TCAGCCCCCT CGTGCAGAAC ATCACCTGTC CCGATGAGGA CGGCACCAGC 
CCTCTGGGCT GGCTGCTGGA CCAGTACCTG GAGTGTCGGG AGGCTGCTCA GAACCCCCAG 
AGTCGTGCGG CAGCTTTCTC CTCACGGGTG CGCCGCCTCA CCCACCTGCT GGTGCACGTG 
GAGCCCTGTG AGGCAGCCCC TCCGGTGGTG GCCACCCCTC AGCCCAACGG CAGAACCAGA 
AGCCATGACT GGAGGTCCTT GTCCATCCGG GGACTTCCAA GCAGTATCAT GAGAAACCTG 
ACCCGCTGTT GGCAGGCCGT GGTGGAGGAG CAGGTGAATA ACTTTCTGAC CTCCTCCTGG 
CGGGATGACG ACTTTGTGCC CCGCTACTGC GAGCAATTTT ATAACCTGCA GAAGTCAAGT 
TCTGAGCTGT TTGGGCCAAG GGCAGCCTTC TTACAGGCGC TGCAGAATGG CTGTGCTGGT 
GCCTTGCTGA AGCTCCCTTT TCTCAGAGCT GCTCATGTGA GTGAGCAGTT TGCTCGGCAC 
ATTGACCAGC GGATCCAGGG CAGCCGGATC GGGGGAGCTC GAGGAATGGA GATGCTGGCA 
CAATTGCAAC GCTGCCTGGA AACTGTCCTA AATTTCTCTG GTCTGGAGAT AGCTACCACC 
TTTGAGCATT ACTACCAGCA CTACATGGCA GACCGTCTCC TGAGCTTGGG CTCCAGCTGG 
CTGGAGGGGG CCGTGCTGGA GCAGATCGGC CTCTGTTTCC CCAACCGCCT CCCCCAGCTC 
ATGTTGCAGA GCCTCAGCAC CTCAGAGGAG CTGCAGCGCC AGTTCCATGT CTATCAGCTC 
CAGCAGCTCG ACCAGGAACT GCTGAAGCTG GAGGACACAG AGAAGAAAAT TCAGGTGGAC 
CATGAGGCCA GTGGCAAGGA GCATAAGAGT GAGAAGGAAG AGGATGCTGG AGCAACAGCA 
GCCATGGCCA TGGCAGAGGG AGAGGAAGAA GAGGAGAATG AGGACCTCTA CTATGAAGGG 
GCAATGCCAG AAGTATCTGT GCTCGTCCTG TCCCCACGCT ACTGGCCTGT GGCCTCCATC 
TGCCACACAC TGAACCCCAG AACCTGCCTG CCTTCCTACC TGAGGGGCAC TTTGAATCGA 
TATTCCAAGT TCTACAACAA GAGTCAGAGC CACCCTGCCG TAACGCAAGA CCTGCAGAAG 
GGACTGCAGT GGACATGGCT GGGCCGGGCT GAGCTGCAGT TTGGGGACCA GACCCTGCAT 
GTGTCCACAG TGCAGATGTG GCTGCTGCTA TATCTCAATG ACCTGAAGGT GTTGTCTGTT 
GAGAGCCTTC GGGAGCTCTC AGGGCTTCCT CTGGACATGC TGAATCAAGC AATTGGGCCT 
CTCACCTCTT CAAGAGGCCC TTTGGATCTC CAGGAGCAGA AGGATGTACC AGGAGGGGTG 
CTGAAGATTC GAGATTGCAG CGAGGAACCC AGGCCAAGGA GGGGCAACGT GTGGCTCGTC 
CCACCTCAGA CATACCTGAA AGCTGAGGGT GAAGAGGGCC GGAACTTGGA GAAGAGACGG 
AACCTTCTGA ACTGCCTCGT TGTCCGAATC CTCAAGGCCC ATGGGGATGA GGGCCTGCAC 
ATTGACCAGC TTGTCTGTCT GGTGCTGGAG GCTTGGCAGA AGGGCCCGTG TCCTCCCAGG 
GGTTTAGTCA GCAGCCTTGG CATGGGGGCT GCATGCTGCA GCACTGACGT CCTCTCCTGC 
ATCCTGCACC TCCTGGGCAA AGGCACACTG AGACGCCATA ATGACCGGCC CCAAATGCTG 
TCCTATGCAG TCCCTGTGAC CGTGATGGAG CCCCACACTG AGTCCTTGAA CCCAGGCTCC 
TCAGGGCCCA ACCCACCCCT CACCTTCCAC ACCCTGCAGA TTCGCTCCCG GGGTGTGCCC 
TATGCCTCCT GCACTGGCAC CCAGAGTTTC TCCACCTTCC GATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020012833.1
CDS	53..5497
Translation	MSRGSGWAADTSHTHPKAGRTVPPAGTRSRPAPAELTRKGAEDSGPAPRSPRATPLARLSVQQRHAPSRLYASTPSGGPGARMVGELRYREFRVPLGPGLHAYPEELIRQRVGHDGHPEYQIRWLILKRGDDGEGGSGQVDCKAEHILLWMSDDEIYANCHKMLGEDGQVIGPSQESAAEAGALDKAVLGEMETEVKSLIQRALRQLEECMGTVPPAPLLHTVHVLSAYASIEPLTGVFKDPRVLDLLMHMLSSPDYQIRWSAGRMIQALSSHDAGEGQLGEEGRAGEGLGRLRDSQDTVAGASDLISTRTQILLSLSQQEAIEKHLDFDSRCALLALFAQATLSEHPMSFEGIQLPQVPGRLLFSLVKRYLHVTSLLDQLNDSAGEPGAQNNSAPEELSVERGRLELEFSMTMGTLISELVQAMRWDWASTRPGSSARSPCSIFQPQPADSSPRLPPSQPQRSFKRSRRFRPRTEFASGNTYALYVRDMLQPGMRVRMLDDYEEISRGDEGEFRQSNSGVPPVQVLWESTGRTYWVHWHMLEILGFEEDIEDAVEADEYQGAVVSGALGRALPSWRWKPMTELYAVPYVLPEDEDTEESEHLTQAEWWELLFFIKKLDGPDRQGVLQLLQENLDGEHILDEEILAELTVPIELVQDLLLTLPQQLDDSALRDLLNCHVYRKYGPEALAGQPAHPTLLEAQQDVLLPRAQGQPQESEDAARVEAKEPPSQSPSAALKSLVEGLGPAGKLLVDLEQALGSGSEGPLESQVKSCLLQLQRQPQPFLELMQSLDTPATNKTLHLTVLRILMKLVDFPEALLLPWHEAMDACMACLRSPNTDREVLQELIFFLHRLTSVSRDYAVVLNQLGARDAISKALEKHLGKLELAQELRDMVFKCEKHAHLYRKLTTNILGGCIQMVLGQIEDHRRTHRPINIPFFDVLLRYLCQGSSVEVKEDKCWQKVEVSSNPHRACRLTDRNPKTYWESKGSAGCHSITLHMRQGVHIRQLTLLVAGEDSSYMPARVVVCGGDSTSSLNTELNSVNVMPCASRVTLLENLTRFWPIIQIRIKRCQQGGIDTRIRGLEVLGPKPTFWPVFREQLCRHTRLFYMVRAQAWSQDIAEDRRSLLHLSSRLNGALRQEQNFADRFLPDDEAAQALGKTCWEALVSPLVQNITCPDEDGTSPLGWLLDQYLECREAAQNPQSRAAAFSSRVRRLTHLLVHVEPCEAAPPVVATPQPNGRTRSHDWRSLSIRGLPSSIMRNLTRCWQAVVEEQVNNFLTSSWRDDDFVPRYCEQFYNLQKSSSELFGPRAAFLQALQNGCAGALLKLPFLRAAHVSEQFARHIDQRIQGSRIGGARGMEMLAQLQRCLETVLNFSGLEIATTFEHYYQHYMADRLLSLGSSWLEGAVLEQIGLCFPNRLPQLMLQSLSTSEELQRQFHVYQLQQLDQELLKLEDTEKKIQVDHEASGKEHKSEKEEDAGATAAMAMAEGEEEEENEDLYYEGAMPEVSVLVLSPRYWPVASICHTLNPRTCLPSYLRGTLNRYSKFYNKSQSHPAVTQDLQKGLQWTWLGRAELQFGDQTLHVSTVQMWLLLYLNDLKVLSVESLRELSGLPLDMLNQAIGPLTSSRGPLDLQEQKDVPGGVLKIRDCSEEPRPRRGNVWLVPPQTYLKAEGEEGRNLEKRRNLLNCLVVRILKAHGDEGLHIDQLVCLVLEAWQKGPCPPRGLVSSLGMGAACCSTDVLSCILHLLGKGTLRRHNDRPQMLSYAVPVTVMEPHTESLNPGSSGPNPPLTFHTLQIRSRGVPYASCTGTQSFSTFR

Target ORF information:

RefSeq Version	XM_020157244.1
Organism	Castor canadensis(American beaver)
Definition	Castor canadensis cullin 7 (Cul7), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020157244.1

ORF Insert Sequence:

ATGAGCCGTG GCTCTGGGTG GGCGGCAGAC ACCTCACACA CACACCCAAA AGCTGGGCGC 
ACGGTGCCGC CCGCTGGGAC CAGATCCCGC CCTGCCCCAG CGGAGCTGAC GCGCAAGGGC 
GCTGAGGACA GCGGCCCGGC CCCTAGGTCC CCGCGCGCCA CACCCCTTGC CCGCCTGTCT 
GTCCAGCAGA GACACGCCCC CTCCCGCCTA TACGCCTCGA CCCCCTCGGG GGGCCCAGGT 
GCCAGGATGG TGGGAGAACT CCGCTACAGG GAATTCAGGG TGCCCCTGGG GCCTGGCTTG 
CATGCCTATC CTGAGGAGCT GATCCGCCAG CGGGTGGGCC ATGACGGGCA TCCTGAGTAC 
CAGATCCGCT GGCTCATCCT CAAGCGCGGA GATGATGGGG AAGGGGGATC TGGTCAAGTG 
GACTGCAAGG CTGAGCACAT CCTGCTGTGG ATGTCTGACG ACGAGATCTA TGCCAACTGC 
CACAAGATGC TAGGCGAGGA CGGCCAAGTC ATCGGGCCCT CCCAGGAATC TGCAGCAGAG 
GCCGGCGCTC TGGACAAAGC CGTGCTGGGG GAGATGGAAA CTGAAGTGAA GTCCCTGATT 
CAGAGGGCCC TTCGGCAGCT GGAGGAGTGT ATGGGAACTG TCCCTCCTGC TCCTCTGCTT 
CACACTGTCC ATGTGCTCAG TGCCTATGCC AGCATCGAGC CCCTCACTGG GGTCTTCAAA 
GACCCAAGAG TCCTGGACCT GCTCATGCAC ATGCTGAGTA GTCCTGACTA CCAGATTCGC 
TGGAGTGCAG GCCGGATGAT ACAAGCCCTG TCCTCCCATG ATGCTGGTGA GGGGCAGCTT 
GGGGAGGAAG GGAGAGCAGG AGAGGGGCTG GGTCGGCTCA GGGACTCACA GGACACTGTG 
GCAGGAGCCT CTGACCTCAT CAGCACCCGG ACCCAGATCC TTCTGTCATT GAGCCAACAA 
GAGGCCATTG AGAAACACCT GGATTTTGAC AGCCGCTGTG CGCTGCTAGC ACTATTTGCA 
CAGGCCACTC TCTCTGAACA TCCCATGTCT TTCGAGGGTA TTCAACTGCC TCAGGTCCCA 
GGAAGGCTGC TCTTCTCCCT TGTGAAACGC TATTTGCATG TCACCTCCCT GCTGGATCAA 
CTGAATGACA GTGCTGGGGA GCCAGGAGCC CAGAACAACT CTGCTCCTGA GGAGTTGAGT 
GTGGAGAGGG GCCGGTTGGA GCTGGAATTC AGTATGACCA TGGGCACCCT GATCTCCGAG 
CTGGTGCAGG CCATGCGCTG GGACTGGGCT TCGACCAGAC CAGGGAGCTC GGCTCGGTCC 
CCCTGTTCCA TCTTCCAGCC TCAGCCTGCA GATAGCAGCC CACGGCTCCC ACCCTCCCAG 
CCCCAGCGTT CCTTCAAGAG GTCAAGACGG TTTCGCCCTC GCACTGAGTT CGCAAGCGGC 
AACACCTATG CCTTATACGT GCGGGACATG CTGCAGCCAG GGATGCGAGT ACGAATGCTG 
GATGACTATG AGGAGATCAG TAGAGGGGAT GAGGGCGAGT TCCGGCAGAG CAATAGTGGA 
GTGCCCCCCG TGCAGGTGTT GTGGGAGTCG ACAGGCCGCA CCTACTGGGT ACACTGGCAC 
ATGCTGGAGA TCCTGGGCTT TGAGGAAGAC ATTGAGGATG CGGTTGAGGC TGATGAGTAC 
CAGGGGGCAG TGGTCAGTGG AGCTCTGGGC AGAGCCTTGC CCTCCTGGCG CTGGAAGCCC 
ATGACTGAGC TCTATGCTGT GCCTTATGTG CTGCCTGAGG ATGAGGACAC TGAGGAGAGT 
GAACACCTGA CCCAAGCCGA GTGGTGGGAG CTCCTCTTTT TCATCAAGAA GTTGGATGGA 
CCTGACCGTC AGGGGGTTCT CCAGCTCCTA CAGGAGAACC TGGATGGGGA GCACATTTTG 
GACGAGGAGA TCCTGGCTGA GCTGACTGTG CCCATTGAGC TGGTCCAGGA TCTGCTATTG 
ACTCTGCCAC AGCAACTTGA TGACAGTGCC CTCAGGGACC TGCTCAACTG CCATGTCTAC 
AGAAAGTATG GGCCCGAGGC CCTGGCAGGG CAGCCAGCTC ATCCAACCCT TCTAGAAGCC 
CAGCAAGATG TGCTCCTGCC GAGAGCCCAG GGCCAGCCCC AGGAATCAGA AGATGCAGCC 
AGAGTGGAAG CAAAAGAACC CCCCTCTCAG AGCCCCAGTG CTGCCCTGAA GTCTCTGGTG 
GAGGGTTTGG GTCCTGCTGG GAAACTCCTT GTAGATTTGG AGCAAGCCCT TGGCTCTGGC 
TCAGAGGGGC CCCTGGAGAG CCAGGTCAAG TCCTGCCTGC TGCAGCTCCA GCGGCAGCCC 
CAGCCTTTCC TCGAATTGAT GCAGAGCCTT GACACTCCAG CCACCAATAA GACCCTGCAC 
CTGACTGTGC TGAGAATTCT GATGAAGCTG GTAGACTTCC CCGAGGCGCT GCTGCTCCCC 
TGGCACGAGG CCATGGATGC CTGCATGGCC TGCCTGCGGT CCCCCAACAC TGACCGAGAG 
GTGCTCCAAG AACTGATCTT CTTCCTGCAC CGCCTGACCT CAGTGAGCAG GGACTATGCT 
GTGGTGCTCA ATCAGCTGGG AGCCCGAGAT GCCATCTCCA AGGCCCTGGA AAAGCACCTG 
GGAAAGCTGG AGCTGGCTCA GGAGCTGAGG GACATGGTGT TCAAGTGTGA GAAGCATGCC 
CACCTCTACA GGAAGCTCAC CACCAACATC CTGGGCGGCT GCATCCAGAT GGTGCTGGGC 
CAGATCGAAG ACCACAGACG AACCCACCGG CCCATCAATA TCCCCTTCTT TGATGTCCTC 
CTCAGATACC TGTGCCAGGG CTCCAGCGTG GAAGTGAAGG AGGACAAGTG CTGGCAGAAG 
GTGGAGGTGT CTTCCAACCC GCACCGGGCC TGCAGACTGA CGGACCGCAA TCCCAAGACC 
TACTGGGAGT CCAAGGGTAG TGCAGGCTGT CACTCCATCA CCCTGCACAT GCGCCAGGGT 
GTCCACATCA GGCAGCTGAC TCTGCTGGTG GCCGGGGAGG ACTCAAGCTA CATGCCGGCT 
CGGGTGGTGG TGTGCGGGGG CGACAGCACC AGCTCTCTTA ACACAGAACT CAACTCGGTA 
AATGTGATGC CTTGTGCCAG CCGGGTGACA CTCCTGGAGA ACCTGACTCG CTTCTGGCCC 
ATCATCCAGA TCCGCATAAA ACGCTGCCAG CAGGGTGGCA TTGACACTCG AATTCGGGGG 
CTGGAAGTCT TGGGTCCCAA GCCCACTTTC TGGCCAGTGT TCCGGGAGCA ACTGTGTCGT 
CACACACGCC TCTTCTACAT GGTTCGGGCG CAGGCCTGGA GCCAGGACAT AGCAGAGGAC 
CGTAGGAGTC TCCTGCACCT GAGCTCTAGG CTCAATGGGG CTCTGCGTCA GGAGCAGAAT 
TTTGCTGATC GTTTCCTCCC TGACGACGAG GCTGCCCAAG CACTGGGCAA GACCTGCTGG 
GAGGCCCTGG TCAGCCCCCT CGTGCAGAAC ATCACCTGTC CCGATGAGGA CGGCACCAGC 
CCTCTGGGCT GGCTGCTGGA CCAGTACCTG GAGTGTCGGG AGGCTGCTCA GAACCCCCAG 
AGTCGTGCGG CAGCTTTCTC CTCACGGGTG CGCCGCCTCA CCCACCTGCT GGTGCACGTG 
GAGCCCTGTG AGGCAGCCCC TCCGGTGGTG GCCACCCCTC AGCCCAACGG CAGAACCAGA 
AGCCATGACT GGAGGTCCTT GTCCATCCGG GGACTTCCAA GCAGTATCAT GAGAAACCTG 
ACCCGCTGTT GGCAGGCCGT GGTGGAGGAG CAGGTGAATA ACTTTCTGAC CTCCTCCTGG 
CGGGATGACG ACTTTGTGCC CCGCTACTGC GAGCAATTTT ATAACCTGCA GAAGTCAAGT 
TCTGAGCTGT TTGGGCCAAG GGCAGCCTTC TTACAGGCGC TGCAGAATGG CTGTGCTGGT 
GCCTTGCTGA AGCTCCCTTT TCTCAGAGCT GCTCATGTGA GTGAGCAGTT TGCTCGGCAC 
ATTGACCAGC GGATCCAGGG CAGCCGGATC GGGGGAGCTC GAGGAATGGA GATGCTGGCA 
CAATTGCAAC GCTGCCTGGA AACTGTCCTA AATTTCTCTG GTCTGGAGAT AGCTACCACC 
TTTGAGCATT ACTACCAGCA CTACATGGCA GACCGTCTCC TGAGCTTGGG CTCCAGCTGG 
CTGGAGGGGG CCGTGCTGGA GCAGATCGGC CTCTGTTTCC CCAACCGCCT CCCCCAGCTC 
ATGTTGCAGA GCCTCAGCAC CTCAGAGGAG CTGCAGCGCC AGTTCCATGT CTATCAGCTC 
CAGCAGCTCG ACCAGGAACT GCTGAAGCTG GAGGACACAG AGAAGAAAAT TCAGGTGGAC 
CATGAGGCCA GTGGCAAGGA GCATAAGAGT GAGAAGGAAG AGGATGCTGG AGCAACAGCA 
GCCATGGCCA TGGCAGAGGG AGAGGAAGAA GAGGAGAATG AGGACCTCTA CTATGAAGGG 
GCAATGCCAG AAGTATCTGT GCTCGTCCTG TCCCCACGCT ACTGGCCTGT GGCCTCCATC 
TGCCACACAC TGAACCCCAG AACCTGCCTG CCTTCCTACC TGAGGGGCAC TTTGAATCGA 
TATTCCAAGT TCTACAACAA GAGTCAGAGC CACCCTGCCG TAACGCAAGA CCTGCAGAAG 
GGACTGCAGT GGACATGGCT GGGCCGGGCT GAGCTGCAGT TTGGGGACCA GACCCTGCAT 
GTGTCCACAG TGCAGATGTG GCTGCTGCTA TATCTCAATG ACCTGAAGGT GTTGTCTGTT 
GAGAGCCTTC GGGAGCTCTC AGGGCTTCCT CTGGACATGC TGAATCAAGC AATTGGGCCT 
CTCACCTCTT CAAGAGGCCC TTTGGATCTC CAGGAGCAGA AGGATGTACC AGGAGGGGTG 
CTGAAGATTC GAGATTGCAG CGAGGAACCC AGGCCAAGGA GGGGCAACGT GTGGCTCGTC 
CCACCTCAGA CATACCTGAA AGCTGAGGGT GAAGAGGGCC GGAACTTGGA GAAGAGACGG 
AACCTTCTGA ACTGCCTCGT TGTCCGAATC CTCAAGGCCC ATGGGGATGA GGGCCTGCAC 
ATTGACCAGC TTGTCTGTCT GGTGCTGGAG GCTTGGCAGA AGGGCCCGTG TCCTCCCAGG 
GGTTTAGTCA GCAGCCTTGG CATGGGGGCT GCATGCTGCA GCACTGACGT CCTCTCCTGC 
ATCCTGCACC TCCTGGGCAA AGGCACACTG AGACGCCATA ATGACCGGCC CCAAATGCTG 
TCCTATGCAG TCCCTGTGAC CGTGATGGAG CCCCACACTG AGTCCTTGAA CCCAGGCTCC 
TCAGGGCCCA ACCCACCCCT CACCTTCCAC ACCCTGCAGA TTCGCTCCCG GGGTGTGCCC 
TATGCCTCCT GCACTGGCAC CCAGAGTTTC TCCACCTTCC GATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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1-877-436-7274

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1-732-210-0262

Cul7 cDNA ORF clone, Castor canadensis(American beaver)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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PHONE

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