LOC101503412 cDNA ORF clone, Cicer arietinum(chickpea)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101503412
Entrez Gene ID	101503412
Full Name	histone-lysine N-methyltransferase ASHH2
Gene Type	protein-coding
Organism	Cicer arietinum(chickpea)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004502484.2	XP_004502541.1	histone-lysine N-methyltransferase ASHH2 isoform X2
XM_004502484.3	XP_004502541.1	histone-lysine N-methyltransferase ASHH2 isoform X2
XM_004502483.2	XP_004502540.1	histone-lysine N-methyltransferase ASHH2 isoform X1
XM_004502482.2	XP_004502539.1	histone-lysine N-methyltransferase ASHH2 isoform X1
XM_004502482.3	XP_004502539.1	histone-lysine N-methyltransferase ASHH2 isoform X1
XM_004502483.3	XP_004502540.1	histone-lysine N-methyltransferase ASHH2 isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC101503412 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101503412 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCi113901	XM_004502483.3 Latest version!	Cicer arietinum histone-lysine N-methyltransferase ASHH2 (LOC101503412), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCi113901	XM_004502482.3 Latest version!	Cicer arietinum histone-lysine N-methyltransferase ASHH2 (LOC101503412), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCi113902	XM_004502484.3 Latest version!	Cicer arietinum histone-lysine N-methyltransferase ASHH2 (LOC101503412), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
View Old Accession Versions >>
OCi113902	XM_004502484.2	Cicer arietinum histone-lysine N-methyltransferase ASHH2 (LOC101503412), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCi113901	XM_004502482.2	Cicer arietinum histone-lysine N-methyltransferase ASHH2 (LOC101503412), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCi113901	XM_004502483.2	Cicer arietinum histone-lysine N-methyltransferase ASHH2 (LOC101503412), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101503412 ( XM_004502483.2 ) cDNA ORF clone, Cicer arietinum(chickpea) -> XP_004502540.1 Cicer arietinum histone-lysine N-methyltransferase ASHH2 (LOC101503412), transcript variant X2, mRNA.

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CloneID	OCi113901
Clone ID Related Accession (Same CDS sequence)	XM_004502483.2 , XM_004502482.2 , XM_004502482.3 , XM_004502483.3
Accession Version	XM_004502483.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5940bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-06-07
Organism	Cicer arietinum(chickpea)
Product	histone-lysine N-methyltransferase ASHH2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004515485.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 8, 2015 this sequence version replaced XM_004502483.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cicer arietinum Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTCGT GTGGTGAACC GGTAATCGTT GGTGATCCGG CACAATTGGA GCAGCCTTTG 
TGCTCGGGTT TTCCGGCACA GTTAGTTTCT GTGCAGGAGT CATGTTCAGA AGAACCTTGC 
GATGTTTTAG ACGTATCTGG TATGGCGGAT GGTTTTTTGG GAGAGGGTGA AGTGGGTTGT 
TCTGCAAGCT ATCTTGATGT TATGGAGGGG TACGGAGATG CTTTGACAAA TGAATGTGAA 
AATGATGATA TGTCACAATT GGAGAAAACT GCTGAAGATG GTTGTTGGAA TAGTTTGGGG 
ATTTGTTCTG AGGACATTGA AGTTTCCGGT CTTAAAAGTG GTGGATTGTG CTTAGAAGGA 
AATTTTAAAG ATGAAGGAAA CCTTGACCTG CCGTTGGAGT CCATAACCTC AAATGATTTG 
CAGAAGCATT GGGCTGAACA GGGTGAGCAG AAGAATGGCA AGAACATTAT GGTATTCTCG 
GTAGGAGATG ATTTGACAGT CGTGGAAGGC AAAAACGATG GTGCTGATTT ACTAGCTGGT 
GCTGTCAACT GTGTGCTTGA TGTTGAGCAT TCTGAAGTGC CTTTGGAATC AGATAGCTGG 
CCAAAGGCTG TGGAAAATTG TGATGCTTTA GATGGGATAG AAGCGAATTC TTGCAGGCAG 
ATATCTTCTT CACTAAATTT TGAAGTGCCG TCAGGTGTCT TGTTTATAGA TGCAAAAGTA 
GAGAGCACAA GTGATAAGCT ACACGATCTA AAAAATGGCG AGGATTGTGA CAGTACTTGT 
GATGAAAAGA TAAGAGCCTT TGTTGATAAA GAAATCACTG TTAATTCATA TGTACAAGCG 
TCGTCCTCAC CAGATACAAA AGAAGAGAGC ACAAGTGATG AGCTACATGA CCAGAAAAAT 
GGTCAGGATT ATAAAAGTAG TTGTGAAGAA AAGTTAAGAG CCTTTGTTGA TAAAGAATCA 
ACTGTTAATT CGTATTTACA AGCATCGTCC TTGCCAGATT TCCATAGGAC CCTGAGGACT 
TCACCTGTAA TTGATTCACA ATGTCAGCCT ACCTTGATGG ATCCAGGGGG TGAATTGAAG 
AATGGCATAC TGCAAACAGA AGATAATTTC TGTACATTGA AGGATTGTTC TGCAGATGGA 
AATGCCAACT CTACCATTAG AAAACAATTT TATCCAGAAT CAGGTCAGTC CTCTTTTGTT 
GTTCTCACCA ATAGCTTTCC AAAGGATGCG CCGGATCTCC TTTCCAAGGG CGATGGTGCT 
TCCATCAACA ATGATTGTGC TGTAGACAAT CCTGGACAAA CAAATAATGA TGGAAAAGAA 
GATGTTGAAG TTGATCATAT TACTGAAAAC ATACTGTTAC CTTCTCAAAG GAGTCAAAGA 
ACCAAATTTG GTAGTAAGAC ACAAACCAGG AAGGCTTCTA GAAAATCCAA GAACAAAGCC 
AGTATGACAC ATCGAGGTGG AGGTATGAAT ATGAACTTAG AGGCTGCTAG AAAGAAAAGA 
AGTTGTTTAT GCAAACCGGC TCGTTCTTCT GTCTGGGGAT CGCTTGGGAA TATTGAACAG 
TTTTTTCAGC ACGAAAACGA GCTGGAGGTT AGTGAAGCCA TGTGCCAGAA TGTGGTGAAA 
GCTAGAAGCA AGTTTCAAGG TGGGGAAATG ATTAAGAATG CTACAAGTTC TAGCTCATTA 
AGTTCAGTTC AAAAGGATAA TGTTCCAACT TCAACTACTC GTTTTCGTCT GAAGATCAAG 
TTTGGAAAAG AAAATGCTTT ACGTTGCTCA AACGTCTTGA TTCCGGAGTC TGTTGGTGGC 
TTGGCTTCTG CTTCCTACTT GGAGTCGGAC CCAGGTTCTC GGAAAGTAGC CAGCAATTCT 
GCAGATAAAT TTTCTGAAGC ATTGGCTTTA AGCAATTTAG AATCATTCAG AAATGACCTG 
GACAAGGATT TTGTTGTTCT AAATGGACAA ATTGTAAATA GCCAGTTAGA AAATTCTAAA 
ATAACGGAGA AGTCAGATGA GGATGCAGTG GAACCATGTC ATGCAGGTCC TCCAGAGAAA 
GTGGTTGAAG CATTAGTTAA GCCAATTAAT AATTCAGTTA TAGATCCCGG AACGTCACCT 
GATTCAGAAG TCATTGATTC AATTCCTGAC GTCCAGGTTG GAGAAAGACA TGAAGATGTA 
CATTTTTCTG TGTTAGGTTC TTCTAAGGAG TTAAATTCTC ATATGGACGT TACCTTCAGG 
AATAGAGGGA AGAAGAAAGA CAAGCTTATT TATTCAGGCA ATTGCATCAC TGAAGATGGA 
TCACAAGGTC AACCAGGGAA TAATAGAGCT AAGCATTCGA AGAATCACAG ACGTAAGAGA 
ACCTCCAGTG ATGTGGTTAG CTCTTTAGAA TTGCCTATTT CCACTGAGCT AAGCAAATCT 
GTAAAGAGTA AAGAATTGTC TGCAGAATCA TCGCCCCTTT CTGGAGATAT TGTACTTGGA 
GGCCCTATGG AGTCTTTGAA AGTTAAAAGT CATGCGATAG TCAAGACAGC TGGCAAATCA 
TCTGCTGACC ATGGTTTTAC TGAACTCCAG GGTTCAAAAA ATTTACCGTC TTCTACAAGA 
CCTTGGGGTC GTAAACTACC TAAAAGTCTT GAACCTAGAA AAGTTAGCAA GGGTAAATCC 
AAAGCTTCTG ACTCGACAAG CAGGAAAAAG CCTACTTCTA GGCCCAAGGA GAGACAGAAA 
GAGCCAATTA ACAGAAGTGA AGTAAAGGGA AAAGATGTCT CTCTCAAAGT TACATGTGAA 
GTGGAAGATC ACCCACATCC AGCAGATATA GTTGGAAATC ATAAGTTGGC TGGTGTTGGA 
AAAATTAATA CCGGTGACAA CAGAGTACCA GTCAGTGTGT CTAATTTGGA CGTGATGCCT 
GGTTTTGGTT TGGAAGAACA GCAGCAGTCA CCTCGTAATG CATGGGTGAG TTGTGATGAC 
TGTCACAAGT GGCGGCGCAT TCCAGCTTTG CTTGCAGACC AGATTGATGA AACTAACTGC 
ACCTGGACTT GCAAGGACAG CAGTGATAAA GCCTATGCTG ATTGTGCTAT CCCTCAAGAA 
AAGTCAAATG CAGAGATTAA TGCAGAGTTA GGATTATCAG ACGCCTCTGG TGAAGAAGAT 
GCATATGGAA ACTCCAAAAC TCATAAGGAG TTGGAATATC AACTGCCTTT GGTTTCCCAA 
GAGTCAACTT TTACCCGCAT TTTCACAAAT GAATTTCTGC ATCGTAACCC AAGAACTCAG 
ACTATTGATG AGGTAATGGT TTGCCATTGC AAGCCACCTC GAGAAGGCAA GATGGGATGT 
GGGGATGAAT GTCTAAACCG GATGCTTAAC ATCGAATGTG TGCAAGGAAC TTGCCCATGT 
GGGGACCGTT GTTCCAATCA GCAGTTCCAA AAACGCAATT ATTCCAGACT GAAGTGGTTT 
AAATGCGGGA AAAAAGGTTA TGGACTGAAG GCACTTGAGC GTGTAGCTGA AGGCCAGTTT 
ATTATTGAAT ACGTTGGAGA GGTGCTTGAC GTGCATGCAT ACGAGGCACG CCAGAGAGAG 
TATGCTTTGA AGGGTCATAG ACATTTCTAT TTTATGACCT TGAACGGCAG TGAGGTGATA 
GATGCAAGTG CAAAAGGAAA TTTGGGGCGT TTCATTAATC ATAGTTGTGA TCCTAATTGT 
CGGACAGAAA AGTGGATGGT GAATGGAGAA ATCTGTATTG GACTGTTTGC ATTGAGGAAT 
ATCAAGCAGG ATGAAGAATT GACATTTGAT TACAACTATG TAAGGGTTTT TGGTGCTGCT 
GCCAAAAAGT GTTATTGTGG TTCACTTCAC TGTCAAGGAT ATATAGGCGG TGCGGATCCA 
AATAACGGTG AGCTTATAGT TCAAGGTGAA TCAGATGATG AATTTCCAGA ACCTATGATG 
CTTTCTGAAA ATGGTGAAAT TGACGACAGC GTACTCATGC CCAAATGCAT TGATAGTGTT 
AATACAAAGT CTTCTAGGCA TTTAATAACA GACAGGGATG TATTGGACAA GTGTACAACT 
GCTATATGTG CAGATGGTTC TCCAGAGGAA GACAGTTCTA CAAACCCTGC CTCTGCTGTT 
TCCCTGTTGC ACAGTTCAGT TGAAGTGGAA GACTCAAAAA GCAATTTACC ATCTTCTGAT 
CGAATAGAAG AAATTTCTCA ACAAATAGAG GATACAACAA GCAAACCCAT GCCTGCTGAC 
AGCAAAGAAT TGCCGAATTC TACTGATAGT AATAGAGAAT CCAAATCTGA AATGGTTGAA 
GTCGGGAATG ATTTTTCACA ATCACATCTG CTTGTGAAGA CTCCTCGACT AAATGCTTCT 
GTTAAAAAGG GTAAGGTAAG GGCTAATGCT GCAAATGCCC TTACAGCCGA GGTGGCGGCC 
CCTCGATTGC CAGTATCATC TATCAAAAAC AAAAAAGTAG AAGGTTCTTC CAATGGACGG 
TTTGAAGCAG TTCAGGGGAA ACTTAATGAG TTGCTGGATG GAAATGGAGG AATAAGCAAA 
AGAAAAGATG CCACCAAAGG CTACTTGAAA CTTTTGCTTC TCACCGTGGC ATCAGGCGAT 
AGAAGCAATG GTGAAGCAAT TCAGAGTAAC CGAGATCTTT CCATGATCCT TGATGCTCTT 
CTGAAAACAA AATCGAGAGC CGTGCTAAAT GATATTATTA GTAAAAATGG TTTACAGATG 
TTACATAAGA TCATGAAGCA GTATAGGCAA GACTTCAAAA AAATTCCAAT ACTACGAAAG 
CTTCTTAAGG TCTTAGAGTA CCTGGCAGCA GGCAAGATTT TGACTCCTGA ACATATCAAT 
GGTGGTCCTC CTTGTCATGG AATGGAGAGG TTTAGGGATT CAATGCTGTC TCTGACAGAG 
CATGATGATA AACAGGTCCA TCAAATTGCT CGAAGCTTCC GAGACAGATG GATTCCAAGA 
CATGGCAGAA AACATGGCTA TATGGACAGG GATGATAACA GGATGGAATC TCACAGAGGT 
TTCAACTCCA ACAGATTTTC AGTATCACAC AGTCATAGGC ACGAGCAGGG ATTAAGACCT 
AAGGAAGCAA CTGATTGTGG TCAGCAGCCA ATGCTTGTGG CAACAGTAGA CGCGCGTGCC 
CAGGAGGGCT GCTCTACACC ATCTTTGGAT GGAGTTGAGA TCAACGGGGC AAAAAAACGT 
AAGCGCAAAA GCCGATGGGA TCAACCAGCA GAAACAAATT CATATTCTGA TGCTATCATT 
AGTTCTATTA ATGAAAGCCA GAACGTTCAT GAAGAAGTTC CACCCGGATT CTCATGTCCA 
ATACGTTCAT TAAACAGTGC ACTAAACTCT GGTACTCCTG CTTTGCAAAA TGCAAGCCAT 
TCTGGATGCC CCCCAAGTTT AGTTATTGGT CAGCCAAAAG AGAAATTCAA TTCTCGTTTG 
CCTGTCTCAT ATGGATTGCC ATGGTCTGTT GCGCAACAAT ATGGAACTCC TCATGCTGAA 
ATCACAGGGT GTTGGATCAC TGCTCCTGGC ATGCCTTTCA ACCCGTTTCC TCCATTACCC 
CCATATCCAC GGGATAACAA AGACTGTCAA CCTTCCAGTA TGGAAATTGA TCAGCCTGCT 
GAAGTTAAGC AATCGGACGC CACTGGTCCG GTTAATTGCT GCTCAGATGA TATGATTCCC 
AGCACAACTG GTGCTAACTC TGAAGACACG AACCTTCAAT GTGAGGATGC TAAACATGAC 
GCTAAGCGAT TGAAGGGTGA TGATTCTGAT GATTTGGGAA AGAAGTACTT TAGACAGCAG 
AAATGGAATA ATTCAAAAAT ACATCGAACA TGGTTCAAAA GGGATGCATG GAAATGTAAT 
GGGAACAGCT CTAGTGGTGA TATTTGCAGT ATAGATGTAG GAGATGTGTC AAAAGAATCA 
AAAGTTACTA GTTATTCAGA GGAGGATGAA ATCTGTAGAG AGGAGAAAGG TGGAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004502540.1
CDS	426..6365
Translation	MGSCGEPVIVGDPAQLEQPLCSGFPAQLVSVQESCSEEPCDVLDVSGMADGFLGEGEVGCSASYLDVMEGYGDALTNECENDDMSQLEKTAEDGCWNSLGICSEDIEVSGLKSGGLCLEGNFKDEGNLDLPLESITSNDLQKHWAEQGEQKNGKNIMVFSVGDDLTVVEGKNDGADLLAGAVNCVLDVEHSEVPLESDSWPKAVENCDALDGIEANSCRQISSSLNFEVPSGVLFIDAKVESTSDKLHDLKNGEDCDSTCDEKIRAFVDKEITVNSYVQASSSPDTKEESTSDELHDQKNGQDYKSSCEEKLRAFVDKESTVNSYLQASSLPDFHRTLRTSPVIDSQCQPTLMDPGGELKNGILQTEDNFCTLKDCSADGNANSTIRKQFYPESGQSSFVVLTNSFPKDAPDLLSKGDGASINNDCAVDNPGQTNNDGKEDVEVDHITENILLPSQRSQRTKFGSKTQTRKASRKSKNKASMTHRGGGMNMNLEAARKKRSCLCKPARSSVWGSLGNIEQFFQHENELEVSEAMCQNVVKARSKFQGGEMIKNATSSSSLSSVQKDNVPTSTTRFRLKIKFGKENALRCSNVLIPESVGGLASASYLESDPGSRKVASNSADKFSEALALSNLESFRNDLDKDFVVLNGQIVNSQLENSKITEKSDEDAVEPCHAGPPEKVVEALVKPINNSVIDPGTSPDSEVIDSIPDVQVGERHEDVHFSVLGSSKELNSHMDVTFRNRGKKKDKLIYSGNCITEDGSQGQPGNNRAKHSKNHRRKRTSSDVVSSLELPISTELSKSVKSKELSAESSPLSGDIVLGGPMESLKVKSHAIVKTAGKSSADHGFTELQGSKNLPSSTRPWGRKLPKSLEPRKVSKGKSKASDSTSRKKPTSRPKERQKEPINRSEVKGKDVSLKVTCEVEDHPHPADIVGNHKLAGVGKINTGDNRVPVSVSNLDVMPGFGLEEQQQSPRNAWVSCDDCHKWRRIPALLADQIDETNCTWTCKDSSDKAYADCAIPQEKSNAEINAELGLSDASGEEDAYGNSKTHKELEYQLPLVSQESTFTRIFTNEFLHRNPRTQTIDEVMVCHCKPPREGKMGCGDECLNRMLNIECVQGTCPCGDRCSNQQFQKRNYSRLKWFKCGKKGYGLKALERVAEGQFIIEYVGEVLDVHAYEARQREYALKGHRHFYFMTLNGSEVIDASAKGNLGRFINHSCDPNCRTEKWMVNGEICIGLFALRNIKQDEELTFDYNYVRVFGAAAKKCYCGSLHCQGYIGGADPNNGELIVQGESDDEFPEPMMLSENGEIDDSVLMPKCIDSVNTKSSRHLITDRDVLDKCTTAICADGSPEEDSSTNPASAVSLLHSSVEVEDSKSNLPSSDRIEEISQQIEDTTSKPMPADSKELPNSTDSNRESKSEMVEVGNDFSQSHLLVKTPRLNASVKKGKVRANAANALTAEVAAPRLPVSSIKNKKVEGSSNGRFEAVQGKLNELLDGNGGISKRKDATKGYLKLLLLTVASGDRSNGEAIQSNRDLSMILDALLKTKSRAVLNDIISKNGLQMLHKIMKQYRQDFKKIPILRKLLKVLEYLAAGKILTPEHINGGPPCHGMERFRDSMLSLTEHDDKQVHQIARSFRDRWIPRHGRKHGYMDRDDNRMESHRGFNSNRFSVSHSHRHEQGLRPKEATDCGQQPMLVATVDARAQEGCSTPSLDGVEINGAKKRKRKSRWDQPAETNSYSDAIISSINESQNVHEEVPPGFSCPIRSLNSALNSGTPALQNASHSGCPPSLVIGQPKEKFNSRLPVSYGLPWSVAQQYGTPHAEITGCWITAPGMPFNPFPPLPPYPRDNKDCQPSSMEIDQPAEVKQSDATGPVNCCSDDMIPSTTGANSEDTNLQCEDAKHDAKRLKGDDSDDLGKKYFRQQKWNNSKIHRTWFKRDAWKCNGNSSSGDICSIDVGDVSKESKVTSYSEEDEICREEKGGK

Target ORF information:

RefSeq Version	XM_004502483.2
Organism	Cicer arietinum(chickpea)
Definition	Cicer arietinum histone-lysine N-methyltransferase ASHH2 (LOC101503412), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004502483.2

ORF Insert Sequence:

ATGGGCTCGT GTGGTGAACC GGTAATCGTT GGTGATCCGG CACAATTGGA GCAGCCTTTG 
TGCTCGGGTT TTCCGGCACA GTTAGTTTCT GTGCAGGAGT CATGTTCAGA AGAACCTTGC 
GATGTTTTAG ACGTATCTGG TATGGCGGAT GGTTTTTTGG GAGAGGGTGA AGTGGGTTGT 
TCTGCAAGCT ATCTTGATGT TATGGAGGGG TACGGAGATG CTTTGACAAA TGAATGTGAA 
AATGATGATA TGTCACAATT GGAGAAAACT GCTGAAGATG GTTGTTGGAA TAGTTTGGGG 
ATTTGTTCTG AGGACATTGA AGTTTCCGGT CTTAAAAGTG GTGGATTGTG CTTAGAAGGA 
AATTTTAAAG ATGAAGGAAA CCTTGACCTG CCGTTGGAGT CCATAACCTC AAATGATTTG 
CAGAAGCATT GGGCTGAACA GGGTGAGCAG AAGAATGGCA AGAACATTAT GGTATTCTCG 
GTAGGAGATG ATTTGACAGT CGTGGAAGGC AAAAACGATG GTGCTGATTT ACTAGCTGGT 
GCTGTCAACT GTGTGCTTGA TGTTGAGCAT TCTGAAGTGC CTTTGGAATC AGATAGCTGG 
CCAAAGGCTG TGGAAAATTG TGATGCTTTA GATGGGATAG AAGCGAATTC TTGCAGGCAG 
ATATCTTCTT CACTAAATTT TGAAGTGCCG TCAGGTGTCT TGTTTATAGA TGCAAAAGTA 
GAGAGCACAA GTGATAAGCT ACACGATCTA AAAAATGGCG AGGATTGTGA CAGTACTTGT 
GATGAAAAGA TAAGAGCCTT TGTTGATAAA GAAATCACTG TTAATTCATA TGTACAAGCG 
TCGTCCTCAC CAGATACAAA AGAAGAGAGC ACAAGTGATG AGCTACATGA CCAGAAAAAT 
GGTCAGGATT ATAAAAGTAG TTGTGAAGAA AAGTTAAGAG CCTTTGTTGA TAAAGAATCA 
ACTGTTAATT CGTATTTACA AGCATCGTCC TTGCCAGATT TCCATAGGAC CCTGAGGACT 
TCACCTGTAA TTGATTCACA ATGTCAGCCT ACCTTGATGG ATCCAGGGGG TGAATTGAAG 
AATGGCATAC TGCAAACAGA AGATAATTTC TGTACATTGA AGGATTGTTC TGCAGATGGA 
AATGCCAACT CTACCATTAG AAAACAATTT TATCCAGAAT CAGGTCAGTC CTCTTTTGTT 
GTTCTCACCA ATAGCTTTCC AAAGGATGCG CCGGATCTCC TTTCCAAGGG CGATGGTGCT 
TCCATCAACA ATGATTGTGC TGTAGACAAT CCTGGACAAA CAAATAATGA TGGAAAAGAA 
GATGTTGAAG TTGATCATAT TACTGAAAAC ATACTGTTAC CTTCTCAAAG GAGTCAAAGA 
ACCAAATTTG GTAGTAAGAC ACAAACCAGG AAGGCTTCTA GAAAATCCAA GAACAAAGCC 
AGTATGACAC ATCGAGGTGG AGGTATGAAT ATGAACTTAG AGGCTGCTAG AAAGAAAAGA 
AGTTGTTTAT GCAAACCGGC TCGTTCTTCT GTCTGGGGAT CGCTTGGGAA TATTGAACAG 
TTTTTTCAGC ACGAAAACGA GCTGGAGGTT AGTGAAGCCA TGTGCCAGAA TGTGGTGAAA 
GCTAGAAGCA AGTTTCAAGG TGGGGAAATG ATTAAGAATG CTACAAGTTC TAGCTCATTA 
AGTTCAGTTC AAAAGGATAA TGTTCCAACT TCAACTACTC GTTTTCGTCT GAAGATCAAG 
TTTGGAAAAG AAAATGCTTT ACGTTGCTCA AACGTCTTGA TTCCGGAGTC TGTTGGTGGC 
TTGGCTTCTG CTTCCTACTT GGAGTCGGAC CCAGGTTCTC GGAAAGTAGC CAGCAATTCT 
GCAGATAAAT TTTCTGAAGC ATTGGCTTTA AGCAATTTAG AATCATTCAG AAATGACCTG 
GACAAGGATT TTGTTGTTCT AAATGGACAA ATTGTAAATA GCCAGTTAGA AAATTCTAAA 
ATAACGGAGA AGTCAGATGA GGATGCAGTG GAACCATGTC ATGCAGGTCC TCCAGAGAAA 
GTGGTTGAAG CATTAGTTAA GCCAATTAAT AATTCAGTTA TAGATCCCGG AACGTCACCT 
GATTCAGAAG TCATTGATTC AATTCCTGAC GTCCAGGTTG GAGAAAGACA TGAAGATGTA 
CATTTTTCTG TGTTAGGTTC TTCTAAGGAG TTAAATTCTC ATATGGACGT TACCTTCAGG 
AATAGAGGGA AGAAGAAAGA CAAGCTTATT TATTCAGGCA ATTGCATCAC TGAAGATGGA 
TCACAAGGTC AACCAGGGAA TAATAGAGCT AAGCATTCGA AGAATCACAG ACGTAAGAGA 
ACCTCCAGTG ATGTGGTTAG CTCTTTAGAA TTGCCTATTT CCACTGAGCT AAGCAAATCT 
GTAAAGAGTA AAGAATTGTC TGCAGAATCA TCGCCCCTTT CTGGAGATAT TGTACTTGGA 
GGCCCTATGG AGTCTTTGAA AGTTAAAAGT CATGCGATAG TCAAGACAGC TGGCAAATCA 
TCTGCTGACC ATGGTTTTAC TGAACTCCAG GGTTCAAAAA ATTTACCGTC TTCTACAAGA 
CCTTGGGGTC GTAAACTACC TAAAAGTCTT GAACCTAGAA AAGTTAGCAA GGGTAAATCC 
AAAGCTTCTG ACTCGACAAG CAGGAAAAAG CCTACTTCTA GGCCCAAGGA GAGACAGAAA 
GAGCCAATTA ACAGAAGTGA AGTAAAGGGA AAAGATGTCT CTCTCAAAGT TACATGTGAA 
GTGGAAGATC ACCCACATCC AGCAGATATA GTTGGAAATC ATAAGTTGGC TGGTGTTGGA 
AAAATTAATA CCGGTGACAA CAGAGTACCA GTCAGTGTGT CTAATTTGGA CGTGATGCCT 
GGTTTTGGTT TGGAAGAACA GCAGCAGTCA CCTCGTAATG CATGGGTGAG TTGTGATGAC 
TGTCACAAGT GGCGGCGCAT TCCAGCTTTG CTTGCAGACC AGATTGATGA AACTAACTGC 
ACCTGGACTT GCAAGGACAG CAGTGATAAA GCCTATGCTG ATTGTGCTAT CCCTCAAGAA 
AAGTCAAATG CAGAGATTAA TGCAGAGTTA GGATTATCAG ACGCCTCTGG TGAAGAAGAT 
GCATATGGAA ACTCCAAAAC TCATAAGGAG TTGGAATATC AACTGCCTTT GGTTTCCCAA 
GAGTCAACTT TTACCCGCAT TTTCACAAAT GAATTTCTGC ATCGTAACCC AAGAACTCAG 
ACTATTGATG AGGTAATGGT TTGCCATTGC AAGCCACCTC GAGAAGGCAA GATGGGATGT 
GGGGATGAAT GTCTAAACCG GATGCTTAAC ATCGAATGTG TGCAAGGAAC TTGCCCATGT 
GGGGACCGTT GTTCCAATCA GCAGTTCCAA AAACGCAATT ATTCCAGACT GAAGTGGTTT 
AAATGCGGGA AAAAAGGTTA TGGACTGAAG GCACTTGAGC GTGTAGCTGA AGGCCAGTTT 
ATTATTGAAT ACGTTGGAGA GGTGCTTGAC GTGCATGCAT ACGAGGCACG CCAGAGAGAG 
TATGCTTTGA AGGGTCATAG ACATTTCTAT TTTATGACCT TGAACGGCAG TGAGGTGATA 
GATGCAAGTG CAAAAGGAAA TTTGGGGCGT TTCATTAATC ATAGTTGTGA TCCTAATTGT 
CGGACAGAAA AGTGGATGGT GAATGGAGAA ATCTGTATTG GACTGTTTGC ATTGAGGAAT 
ATCAAGCAGG ATGAAGAATT GACATTTGAT TACAACTATG TAAGGGTTTT TGGTGCTGCT 
GCCAAAAAGT GTTATTGTGG TTCACTTCAC TGTCAAGGAT ATATAGGCGG TGCGGATCCA 
AATAACGGTG AGCTTATAGT TCAAGGTGAA TCAGATGATG AATTTCCAGA ACCTATGATG 
CTTTCTGAAA ATGGTGAAAT TGACGACAGC GTACTCATGC CCAAATGCAT TGATAGTGTT 
AATACAAAGT CTTCTAGGCA TTTAATAACA GACAGGGATG TATTGGACAA GTGTACAACT 
GCTATATGTG CAGATGGTTC TCCAGAGGAA GACAGTTCTA CAAACCCTGC CTCTGCTGTT 
TCCCTGTTGC ACAGTTCAGT TGAAGTGGAA GACTCAAAAA GCAATTTACC ATCTTCTGAT 
CGAATAGAAG AAATTTCTCA ACAAATAGAG GATACAACAA GCAAACCCAT GCCTGCTGAC 
AGCAAAGAAT TGCCGAATTC TACTGATAGT AATAGAGAAT CCAAATCTGA AATGGTTGAA 
GTCGGGAATG ATTTTTCACA ATCACATCTG CTTGTGAAGA CTCCTCGACT AAATGCTTCT 
GTTAAAAAGG GTAAGGTAAG GGCTAATGCT GCAAATGCCC TTACAGCCGA GGTGGCGGCC 
CCTCGATTGC CAGTATCATC TATCAAAAAC AAAAAAGTAG AAGGTTCTTC CAATGGACGG 
TTTGAAGCAG TTCAGGGGAA ACTTAATGAG TTGCTGGATG GAAATGGAGG AATAAGCAAA 
AGAAAAGATG CCACCAAAGG CTACTTGAAA CTTTTGCTTC TCACCGTGGC ATCAGGCGAT 
AGAAGCAATG GTGAAGCAAT TCAGAGTAAC CGAGATCTTT CCATGATCCT TGATGCTCTT 
CTGAAAACAA AATCGAGAGC CGTGCTAAAT GATATTATTA GTAAAAATGG TTTACAGATG 
TTACATAAGA TCATGAAGCA GTATAGGCAA GACTTCAAAA AAATTCCAAT ACTACGAAAG 
CTTCTTAAGG TCTTAGAGTA CCTGGCAGCA GGCAAGATTT TGACTCCTGA ACATATCAAT 
GGTGGTCCTC CTTGTCATGG AATGGAGAGG TTTAGGGATT CAATGCTGTC TCTGACAGAG 
CATGATGATA AACAGGTCCA TCAAATTGCT CGAAGCTTCC GAGACAGATG GATTCCAAGA 
CATGGCAGAA AACATGGCTA TATGGACAGG GATGATAACA GGATGGAATC TCACAGAGGT 
TTCAACTCCA ACAGATTTTC AGTATCACAC AGTCATAGGC ACGAGCAGGG ATTAAGACCT 
AAGGAAGCAA CTGATTGTGG TCAGCAGCCA ATGCTTGTGG CAACAGTAGA CGCGCGTGCC 
CAGGAGGGCT GCTCTACACC ATCTTTGGAT GGAGTTGAGA TCAACGGGGC AAAAAAACGT 
AAGCGCAAAA GCCGATGGGA TCAACCAGCA GAAACAAATT CATATTCTGA TGCTATCATT 
AGTTCTATTA ATGAAAGCCA GAACGTTCAT GAAGAAGTTC CACCCGGATT CTCATGTCCA 
ATACGTTCAT TAAACAGTGC ACTAAACTCT GGTACTCCTG CTTTGCAAAA TGCAAGCCAT 
TCTGGATGCC CCCCAAGTTT AGTTATTGGT CAGCCAAAAG AGAAATTCAA TTCTCGTTTG 
CCTGTCTCAT ATGGATTGCC ATGGTCTGTT GCGCAACAAT ATGGAACTCC TCATGCTGAA 
ATCACAGGGT GTTGGATCAC TGCTCCTGGC ATGCCTTTCA ACCCGTTTCC TCCATTACCC 
CCATATCCAC GGGATAACAA AGACTGTCAA CCTTCCAGTA TGGAAATTGA TCAGCCTGCT 
GAAGTTAAGC AATCGGACGC CACTGGTCCG GTTAATTGCT GCTCAGATGA TATGATTCCC 
AGCACAACTG GTGCTAACTC TGAAGACACG AACCTTCAAT GTGAGGATGC TAAACATGAC 
GCTAAGCGAT TGAAGGGTGA TGATTCTGAT GATTTGGGAA AGAAGTACTT TAGACAGCAG 
AAATGGAATA ATTCAAAAAT ACATCGAACA TGGTTCAAAA GGGATGCATG GAAATGTAAT 
GGGAACAGCT CTAGTGGTGA TATTTGCAGT ATAGATGTAG GAGATGTGTC AAAAGAATCA 
AAAGTTACTA GTTATTCAGA GGAGGATGAA ATCTGTAGAG AGGAGAAAGG TGGAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101503412 ( XM_004502482.2 ) cDNA ORF clone, Cicer arietinum(chickpea) -> XP_004502539.1 Cicer arietinum histone-lysine N-methyltransferase ASHH2 (LOC101503412), transcript variant X1, mRNA.

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CloneID	OCi113901
Clone ID Related Accession (Same CDS sequence)	XM_004502483.2 , XM_004502482.2 , XM_004502482.3 , XM_004502483.3
Accession Version	XM_004502482.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5940bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-06-07
Organism	Cicer arietinum(chickpea)
Product	histone-lysine N-methyltransferase ASHH2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004515485.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 8, 2015 this sequence version replaced XM_004502482.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cicer arietinum Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTCGT GTGGTGAACC GGTAATCGTT GGTGATCCGG CACAATTGGA GCAGCCTTTG 
TGCTCGGGTT TTCCGGCACA GTTAGTTTCT GTGCAGGAGT CATGTTCAGA AGAACCTTGC 
GATGTTTTAG ACGTATCTGG TATGGCGGAT GGTTTTTTGG GAGAGGGTGA AGTGGGTTGT 
TCTGCAAGCT ATCTTGATGT TATGGAGGGG TACGGAGATG CTTTGACAAA TGAATGTGAA 
AATGATGATA TGTCACAATT GGAGAAAACT GCTGAAGATG GTTGTTGGAA TAGTTTGGGG 
ATTTGTTCTG AGGACATTGA AGTTTCCGGT CTTAAAAGTG GTGGATTGTG CTTAGAAGGA 
AATTTTAAAG ATGAAGGAAA CCTTGACCTG CCGTTGGAGT CCATAACCTC AAATGATTTG 
CAGAAGCATT GGGCTGAACA GGGTGAGCAG AAGAATGGCA AGAACATTAT GGTATTCTCG 
GTAGGAGATG ATTTGACAGT CGTGGAAGGC AAAAACGATG GTGCTGATTT ACTAGCTGGT 
GCTGTCAACT GTGTGCTTGA TGTTGAGCAT TCTGAAGTGC CTTTGGAATC AGATAGCTGG 
CCAAAGGCTG TGGAAAATTG TGATGCTTTA GATGGGATAG AAGCGAATTC TTGCAGGCAG 
ATATCTTCTT CACTAAATTT TGAAGTGCCG TCAGGTGTCT TGTTTATAGA TGCAAAAGTA 
GAGAGCACAA GTGATAAGCT ACACGATCTA AAAAATGGCG AGGATTGTGA CAGTACTTGT 
GATGAAAAGA TAAGAGCCTT TGTTGATAAA GAAATCACTG TTAATTCATA TGTACAAGCG 
TCGTCCTCAC CAGATACAAA AGAAGAGAGC ACAAGTGATG AGCTACATGA CCAGAAAAAT 
GGTCAGGATT ATAAAAGTAG TTGTGAAGAA AAGTTAAGAG CCTTTGTTGA TAAAGAATCA 
ACTGTTAATT CGTATTTACA AGCATCGTCC TTGCCAGATT TCCATAGGAC CCTGAGGACT 
TCACCTGTAA TTGATTCACA ATGTCAGCCT ACCTTGATGG ATCCAGGGGG TGAATTGAAG 
AATGGCATAC TGCAAACAGA AGATAATTTC TGTACATTGA AGGATTGTTC TGCAGATGGA 
AATGCCAACT CTACCATTAG AAAACAATTT TATCCAGAAT CAGGTCAGTC CTCTTTTGTT 
GTTCTCACCA ATAGCTTTCC AAAGGATGCG CCGGATCTCC TTTCCAAGGG CGATGGTGCT 
TCCATCAACA ATGATTGTGC TGTAGACAAT CCTGGACAAA CAAATAATGA TGGAAAAGAA 
GATGTTGAAG TTGATCATAT TACTGAAAAC ATACTGTTAC CTTCTCAAAG GAGTCAAAGA 
ACCAAATTTG GTAGTAAGAC ACAAACCAGG AAGGCTTCTA GAAAATCCAA GAACAAAGCC 
AGTATGACAC ATCGAGGTGG AGGTATGAAT ATGAACTTAG AGGCTGCTAG AAAGAAAAGA 
AGTTGTTTAT GCAAACCGGC TCGTTCTTCT GTCTGGGGAT CGCTTGGGAA TATTGAACAG 
TTTTTTCAGC ACGAAAACGA GCTGGAGGTT AGTGAAGCCA TGTGCCAGAA TGTGGTGAAA 
GCTAGAAGCA AGTTTCAAGG TGGGGAAATG ATTAAGAATG CTACAAGTTC TAGCTCATTA 
AGTTCAGTTC AAAAGGATAA TGTTCCAACT TCAACTACTC GTTTTCGTCT GAAGATCAAG 
TTTGGAAAAG AAAATGCTTT ACGTTGCTCA AACGTCTTGA TTCCGGAGTC TGTTGGTGGC 
TTGGCTTCTG CTTCCTACTT GGAGTCGGAC CCAGGTTCTC GGAAAGTAGC CAGCAATTCT 
GCAGATAAAT TTTCTGAAGC ATTGGCTTTA AGCAATTTAG AATCATTCAG AAATGACCTG 
GACAAGGATT TTGTTGTTCT AAATGGACAA ATTGTAAATA GCCAGTTAGA AAATTCTAAA 
ATAACGGAGA AGTCAGATGA GGATGCAGTG GAACCATGTC ATGCAGGTCC TCCAGAGAAA 
GTGGTTGAAG CATTAGTTAA GCCAATTAAT AATTCAGTTA TAGATCCCGG AACGTCACCT 
GATTCAGAAG TCATTGATTC AATTCCTGAC GTCCAGGTTG GAGAAAGACA TGAAGATGTA 
CATTTTTCTG TGTTAGGTTC TTCTAAGGAG TTAAATTCTC ATATGGACGT TACCTTCAGG 
AATAGAGGGA AGAAGAAAGA CAAGCTTATT TATTCAGGCA ATTGCATCAC TGAAGATGGA 
TCACAAGGTC AACCAGGGAA TAATAGAGCT AAGCATTCGA AGAATCACAG ACGTAAGAGA 
ACCTCCAGTG ATGTGGTTAG CTCTTTAGAA TTGCCTATTT CCACTGAGCT AAGCAAATCT 
GTAAAGAGTA AAGAATTGTC TGCAGAATCA TCGCCCCTTT CTGGAGATAT TGTACTTGGA 
GGCCCTATGG AGTCTTTGAA AGTTAAAAGT CATGCGATAG TCAAGACAGC TGGCAAATCA 
TCTGCTGACC ATGGTTTTAC TGAACTCCAG GGTTCAAAAA ATTTACCGTC TTCTACAAGA 
CCTTGGGGTC GTAAACTACC TAAAAGTCTT GAACCTAGAA AAGTTAGCAA GGGTAAATCC 
AAAGCTTCTG ACTCGACAAG CAGGAAAAAG CCTACTTCTA GGCCCAAGGA GAGACAGAAA 
GAGCCAATTA ACAGAAGTGA AGTAAAGGGA AAAGATGTCT CTCTCAAAGT TACATGTGAA 
GTGGAAGATC ACCCACATCC AGCAGATATA GTTGGAAATC ATAAGTTGGC TGGTGTTGGA 
AAAATTAATA CCGGTGACAA CAGAGTACCA GTCAGTGTGT CTAATTTGGA CGTGATGCCT 
GGTTTTGGTT TGGAAGAACA GCAGCAGTCA CCTCGTAATG CATGGGTGAG TTGTGATGAC 
TGTCACAAGT GGCGGCGCAT TCCAGCTTTG CTTGCAGACC AGATTGATGA AACTAACTGC 
ACCTGGACTT GCAAGGACAG CAGTGATAAA GCCTATGCTG ATTGTGCTAT CCCTCAAGAA 
AAGTCAAATG CAGAGATTAA TGCAGAGTTA GGATTATCAG ACGCCTCTGG TGAAGAAGAT 
GCATATGGAA ACTCCAAAAC TCATAAGGAG TTGGAATATC AACTGCCTTT GGTTTCCCAA 
GAGTCAACTT TTACCCGCAT TTTCACAAAT GAATTTCTGC ATCGTAACCC AAGAACTCAG 
ACTATTGATG AGGTAATGGT TTGCCATTGC AAGCCACCTC GAGAAGGCAA GATGGGATGT 
GGGGATGAAT GTCTAAACCG GATGCTTAAC ATCGAATGTG TGCAAGGAAC TTGCCCATGT 
GGGGACCGTT GTTCCAATCA GCAGTTCCAA AAACGCAATT ATTCCAGACT GAAGTGGTTT 
AAATGCGGGA AAAAAGGTTA TGGACTGAAG GCACTTGAGC GTGTAGCTGA AGGCCAGTTT 
ATTATTGAAT ACGTTGGAGA GGTGCTTGAC GTGCATGCAT ACGAGGCACG CCAGAGAGAG 
TATGCTTTGA AGGGTCATAG ACATTTCTAT TTTATGACCT TGAACGGCAG TGAGGTGATA 
GATGCAAGTG CAAAAGGAAA TTTGGGGCGT TTCATTAATC ATAGTTGTGA TCCTAATTGT 
CGGACAGAAA AGTGGATGGT GAATGGAGAA ATCTGTATTG GACTGTTTGC ATTGAGGAAT 
ATCAAGCAGG ATGAAGAATT GACATTTGAT TACAACTATG TAAGGGTTTT TGGTGCTGCT 
GCCAAAAAGT GTTATTGTGG TTCACTTCAC TGTCAAGGAT ATATAGGCGG TGCGGATCCA 
AATAACGGTG AGCTTATAGT TCAAGGTGAA TCAGATGATG AATTTCCAGA ACCTATGATG 
CTTTCTGAAA ATGGTGAAAT TGACGACAGC GTACTCATGC CCAAATGCAT TGATAGTGTT 
AATACAAAGT CTTCTAGGCA TTTAATAACA GACAGGGATG TATTGGACAA GTGTACAACT 
GCTATATGTG CAGATGGTTC TCCAGAGGAA GACAGTTCTA CAAACCCTGC CTCTGCTGTT 
TCCCTGTTGC ACAGTTCAGT TGAAGTGGAA GACTCAAAAA GCAATTTACC ATCTTCTGAT 
CGAATAGAAG AAATTTCTCA ACAAATAGAG GATACAACAA GCAAACCCAT GCCTGCTGAC 
AGCAAAGAAT TGCCGAATTC TACTGATAGT AATAGAGAAT CCAAATCTGA AATGGTTGAA 
GTCGGGAATG ATTTTTCACA ATCACATCTG CTTGTGAAGA CTCCTCGACT AAATGCTTCT 
GTTAAAAAGG GTAAGGTAAG GGCTAATGCT GCAAATGCCC TTACAGCCGA GGTGGCGGCC 
CCTCGATTGC CAGTATCATC TATCAAAAAC AAAAAAGTAG AAGGTTCTTC CAATGGACGG 
TTTGAAGCAG TTCAGGGGAA ACTTAATGAG TTGCTGGATG GAAATGGAGG AATAAGCAAA 
AGAAAAGATG CCACCAAAGG CTACTTGAAA CTTTTGCTTC TCACCGTGGC ATCAGGCGAT 
AGAAGCAATG GTGAAGCAAT TCAGAGTAAC CGAGATCTTT CCATGATCCT TGATGCTCTT 
CTGAAAACAA AATCGAGAGC CGTGCTAAAT GATATTATTA GTAAAAATGG TTTACAGATG 
TTACATAAGA TCATGAAGCA GTATAGGCAA GACTTCAAAA AAATTCCAAT ACTACGAAAG 
CTTCTTAAGG TCTTAGAGTA CCTGGCAGCA GGCAAGATTT TGACTCCTGA ACATATCAAT 
GGTGGTCCTC CTTGTCATGG AATGGAGAGG TTTAGGGATT CAATGCTGTC TCTGACAGAG 
CATGATGATA AACAGGTCCA TCAAATTGCT CGAAGCTTCC GAGACAGATG GATTCCAAGA 
CATGGCAGAA AACATGGCTA TATGGACAGG GATGATAACA GGATGGAATC TCACAGAGGT 
TTCAACTCCA ACAGATTTTC AGTATCACAC AGTCATAGGC ACGAGCAGGG ATTAAGACCT 
AAGGAAGCAA CTGATTGTGG TCAGCAGCCA ATGCTTGTGG CAACAGTAGA CGCGCGTGCC 
CAGGAGGGCT GCTCTACACC ATCTTTGGAT GGAGTTGAGA TCAACGGGGC AAAAAAACGT 
AAGCGCAAAA GCCGATGGGA TCAACCAGCA GAAACAAATT CATATTCTGA TGCTATCATT 
AGTTCTATTA ATGAAAGCCA GAACGTTCAT GAAGAAGTTC CACCCGGATT CTCATGTCCA 
ATACGTTCAT TAAACAGTGC ACTAAACTCT GGTACTCCTG CTTTGCAAAA TGCAAGCCAT 
TCTGGATGCC CCCCAAGTTT AGTTATTGGT CAGCCAAAAG AGAAATTCAA TTCTCGTTTG 
CCTGTCTCAT ATGGATTGCC ATGGTCTGTT GCGCAACAAT ATGGAACTCC TCATGCTGAA 
ATCACAGGGT GTTGGATCAC TGCTCCTGGC ATGCCTTTCA ACCCGTTTCC TCCATTACCC 
CCATATCCAC GGGATAACAA AGACTGTCAA CCTTCCAGTA TGGAAATTGA TCAGCCTGCT 
GAAGTTAAGC AATCGGACGC CACTGGTCCG GTTAATTGCT GCTCAGATGA TATGATTCCC 
AGCACAACTG GTGCTAACTC TGAAGACACG AACCTTCAAT GTGAGGATGC TAAACATGAC 
GCTAAGCGAT TGAAGGGTGA TGATTCTGAT GATTTGGGAA AGAAGTACTT TAGACAGCAG 
AAATGGAATA ATTCAAAAAT ACATCGAACA TGGTTCAAAA GGGATGCATG GAAATGTAAT 
GGGAACAGCT CTAGTGGTGA TATTTGCAGT ATAGATGTAG GAGATGTGTC AAAAGAATCA 
AAAGTTACTA GTTATTCAGA GGAGGATGAA ATCTGTAGAG AGGAGAAAGG TGGAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004502539.1
CDS	364..6303
Translation	MGSCGEPVIVGDPAQLEQPLCSGFPAQLVSVQESCSEEPCDVLDVSGMADGFLGEGEVGCSASYLDVMEGYGDALTNECENDDMSQLEKTAEDGCWNSLGICSEDIEVSGLKSGGLCLEGNFKDEGNLDLPLESITSNDLQKHWAEQGEQKNGKNIMVFSVGDDLTVVEGKNDGADLLAGAVNCVLDVEHSEVPLESDSWPKAVENCDALDGIEANSCRQISSSLNFEVPSGVLFIDAKVESTSDKLHDLKNGEDCDSTCDEKIRAFVDKEITVNSYVQASSSPDTKEESTSDELHDQKNGQDYKSSCEEKLRAFVDKESTVNSYLQASSLPDFHRTLRTSPVIDSQCQPTLMDPGGELKNGILQTEDNFCTLKDCSADGNANSTIRKQFYPESGQSSFVVLTNSFPKDAPDLLSKGDGASINNDCAVDNPGQTNNDGKEDVEVDHITENILLPSQRSQRTKFGSKTQTRKASRKSKNKASMTHRGGGMNMNLEAARKKRSCLCKPARSSVWGSLGNIEQFFQHENELEVSEAMCQNVVKARSKFQGGEMIKNATSSSSLSSVQKDNVPTSTTRFRLKIKFGKENALRCSNVLIPESVGGLASASYLESDPGSRKVASNSADKFSEALALSNLESFRNDLDKDFVVLNGQIVNSQLENSKITEKSDEDAVEPCHAGPPEKVVEALVKPINNSVIDPGTSPDSEVIDSIPDVQVGERHEDVHFSVLGSSKELNSHMDVTFRNRGKKKDKLIYSGNCITEDGSQGQPGNNRAKHSKNHRRKRTSSDVVSSLELPISTELSKSVKSKELSAESSPLSGDIVLGGPMESLKVKSHAIVKTAGKSSADHGFTELQGSKNLPSSTRPWGRKLPKSLEPRKVSKGKSKASDSTSRKKPTSRPKERQKEPINRSEVKGKDVSLKVTCEVEDHPHPADIVGNHKLAGVGKINTGDNRVPVSVSNLDVMPGFGLEEQQQSPRNAWVSCDDCHKWRRIPALLADQIDETNCTWTCKDSSDKAYADCAIPQEKSNAEINAELGLSDASGEEDAYGNSKTHKELEYQLPLVSQESTFTRIFTNEFLHRNPRTQTIDEVMVCHCKPPREGKMGCGDECLNRMLNIECVQGTCPCGDRCSNQQFQKRNYSRLKWFKCGKKGYGLKALERVAEGQFIIEYVGEVLDVHAYEARQREYALKGHRHFYFMTLNGSEVIDASAKGNLGRFINHSCDPNCRTEKWMVNGEICIGLFALRNIKQDEELTFDYNYVRVFGAAAKKCYCGSLHCQGYIGGADPNNGELIVQGESDDEFPEPMMLSENGEIDDSVLMPKCIDSVNTKSSRHLITDRDVLDKCTTAICADGSPEEDSSTNPASAVSLLHSSVEVEDSKSNLPSSDRIEEISQQIEDTTSKPMPADSKELPNSTDSNRESKSEMVEVGNDFSQSHLLVKTPRLNASVKKGKVRANAANALTAEVAAPRLPVSSIKNKKVEGSSNGRFEAVQGKLNELLDGNGGISKRKDATKGYLKLLLLTVASGDRSNGEAIQSNRDLSMILDALLKTKSRAVLNDIISKNGLQMLHKIMKQYRQDFKKIPILRKLLKVLEYLAAGKILTPEHINGGPPCHGMERFRDSMLSLTEHDDKQVHQIARSFRDRWIPRHGRKHGYMDRDDNRMESHRGFNSNRFSVSHSHRHEQGLRPKEATDCGQQPMLVATVDARAQEGCSTPSLDGVEINGAKKRKRKSRWDQPAETNSYSDAIISSINESQNVHEEVPPGFSCPIRSLNSALNSGTPALQNASHSGCPPSLVIGQPKEKFNSRLPVSYGLPWSVAQQYGTPHAEITGCWITAPGMPFNPFPPLPPYPRDNKDCQPSSMEIDQPAEVKQSDATGPVNCCSDDMIPSTTGANSEDTNLQCEDAKHDAKRLKGDDSDDLGKKYFRQQKWNNSKIHRTWFKRDAWKCNGNSSSGDICSIDVGDVSKESKVTSYSEEDEICREEKGGK

Target ORF information:

RefSeq Version	XM_004502482.2
Organism	Cicer arietinum(chickpea)
Definition	Cicer arietinum histone-lysine N-methyltransferase ASHH2 (LOC101503412), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004502482.2

ORF Insert Sequence:

ATGGGCTCGT GTGGTGAACC GGTAATCGTT GGTGATCCGG CACAATTGGA GCAGCCTTTG 
TGCTCGGGTT TTCCGGCACA GTTAGTTTCT GTGCAGGAGT CATGTTCAGA AGAACCTTGC 
GATGTTTTAG ACGTATCTGG TATGGCGGAT GGTTTTTTGG GAGAGGGTGA AGTGGGTTGT 
TCTGCAAGCT ATCTTGATGT TATGGAGGGG TACGGAGATG CTTTGACAAA TGAATGTGAA 
AATGATGATA TGTCACAATT GGAGAAAACT GCTGAAGATG GTTGTTGGAA TAGTTTGGGG 
ATTTGTTCTG AGGACATTGA AGTTTCCGGT CTTAAAAGTG GTGGATTGTG CTTAGAAGGA 
AATTTTAAAG ATGAAGGAAA CCTTGACCTG CCGTTGGAGT CCATAACCTC AAATGATTTG 
CAGAAGCATT GGGCTGAACA GGGTGAGCAG AAGAATGGCA AGAACATTAT GGTATTCTCG 
GTAGGAGATG ATTTGACAGT CGTGGAAGGC AAAAACGATG GTGCTGATTT ACTAGCTGGT 
GCTGTCAACT GTGTGCTTGA TGTTGAGCAT TCTGAAGTGC CTTTGGAATC AGATAGCTGG 
CCAAAGGCTG TGGAAAATTG TGATGCTTTA GATGGGATAG AAGCGAATTC TTGCAGGCAG 
ATATCTTCTT CACTAAATTT TGAAGTGCCG TCAGGTGTCT TGTTTATAGA TGCAAAAGTA 
GAGAGCACAA GTGATAAGCT ACACGATCTA AAAAATGGCG AGGATTGTGA CAGTACTTGT 
GATGAAAAGA TAAGAGCCTT TGTTGATAAA GAAATCACTG TTAATTCATA TGTACAAGCG 
TCGTCCTCAC CAGATACAAA AGAAGAGAGC ACAAGTGATG AGCTACATGA CCAGAAAAAT 
GGTCAGGATT ATAAAAGTAG TTGTGAAGAA AAGTTAAGAG CCTTTGTTGA TAAAGAATCA 
ACTGTTAATT CGTATTTACA AGCATCGTCC TTGCCAGATT TCCATAGGAC CCTGAGGACT 
TCACCTGTAA TTGATTCACA ATGTCAGCCT ACCTTGATGG ATCCAGGGGG TGAATTGAAG 
AATGGCATAC TGCAAACAGA AGATAATTTC TGTACATTGA AGGATTGTTC TGCAGATGGA 
AATGCCAACT CTACCATTAG AAAACAATTT TATCCAGAAT CAGGTCAGTC CTCTTTTGTT 
GTTCTCACCA ATAGCTTTCC AAAGGATGCG CCGGATCTCC TTTCCAAGGG CGATGGTGCT 
TCCATCAACA ATGATTGTGC TGTAGACAAT CCTGGACAAA CAAATAATGA TGGAAAAGAA 
GATGTTGAAG TTGATCATAT TACTGAAAAC ATACTGTTAC CTTCTCAAAG GAGTCAAAGA 
ACCAAATTTG GTAGTAAGAC ACAAACCAGG AAGGCTTCTA GAAAATCCAA GAACAAAGCC 
AGTATGACAC ATCGAGGTGG AGGTATGAAT ATGAACTTAG AGGCTGCTAG AAAGAAAAGA 
AGTTGTTTAT GCAAACCGGC TCGTTCTTCT GTCTGGGGAT CGCTTGGGAA TATTGAACAG 
TTTTTTCAGC ACGAAAACGA GCTGGAGGTT AGTGAAGCCA TGTGCCAGAA TGTGGTGAAA 
GCTAGAAGCA AGTTTCAAGG TGGGGAAATG ATTAAGAATG CTACAAGTTC TAGCTCATTA 
AGTTCAGTTC AAAAGGATAA TGTTCCAACT TCAACTACTC GTTTTCGTCT GAAGATCAAG 
TTTGGAAAAG AAAATGCTTT ACGTTGCTCA AACGTCTTGA TTCCGGAGTC TGTTGGTGGC 
TTGGCTTCTG CTTCCTACTT GGAGTCGGAC CCAGGTTCTC GGAAAGTAGC CAGCAATTCT 
GCAGATAAAT TTTCTGAAGC ATTGGCTTTA AGCAATTTAG AATCATTCAG AAATGACCTG 
GACAAGGATT TTGTTGTTCT AAATGGACAA ATTGTAAATA GCCAGTTAGA AAATTCTAAA 
ATAACGGAGA AGTCAGATGA GGATGCAGTG GAACCATGTC ATGCAGGTCC TCCAGAGAAA 
GTGGTTGAAG CATTAGTTAA GCCAATTAAT AATTCAGTTA TAGATCCCGG AACGTCACCT 
GATTCAGAAG TCATTGATTC AATTCCTGAC GTCCAGGTTG GAGAAAGACA TGAAGATGTA 
CATTTTTCTG TGTTAGGTTC TTCTAAGGAG TTAAATTCTC ATATGGACGT TACCTTCAGG 
AATAGAGGGA AGAAGAAAGA CAAGCTTATT TATTCAGGCA ATTGCATCAC TGAAGATGGA 
TCACAAGGTC AACCAGGGAA TAATAGAGCT AAGCATTCGA AGAATCACAG ACGTAAGAGA 
ACCTCCAGTG ATGTGGTTAG CTCTTTAGAA TTGCCTATTT CCACTGAGCT AAGCAAATCT 
GTAAAGAGTA AAGAATTGTC TGCAGAATCA TCGCCCCTTT CTGGAGATAT TGTACTTGGA 
GGCCCTATGG AGTCTTTGAA AGTTAAAAGT CATGCGATAG TCAAGACAGC TGGCAAATCA 
TCTGCTGACC ATGGTTTTAC TGAACTCCAG GGTTCAAAAA ATTTACCGTC TTCTACAAGA 
CCTTGGGGTC GTAAACTACC TAAAAGTCTT GAACCTAGAA AAGTTAGCAA GGGTAAATCC 
AAAGCTTCTG ACTCGACAAG CAGGAAAAAG CCTACTTCTA GGCCCAAGGA GAGACAGAAA 
GAGCCAATTA ACAGAAGTGA AGTAAAGGGA AAAGATGTCT CTCTCAAAGT TACATGTGAA 
GTGGAAGATC ACCCACATCC AGCAGATATA GTTGGAAATC ATAAGTTGGC TGGTGTTGGA 
AAAATTAATA CCGGTGACAA CAGAGTACCA GTCAGTGTGT CTAATTTGGA CGTGATGCCT 
GGTTTTGGTT TGGAAGAACA GCAGCAGTCA CCTCGTAATG CATGGGTGAG TTGTGATGAC 
TGTCACAAGT GGCGGCGCAT TCCAGCTTTG CTTGCAGACC AGATTGATGA AACTAACTGC 
ACCTGGACTT GCAAGGACAG CAGTGATAAA GCCTATGCTG ATTGTGCTAT CCCTCAAGAA 
AAGTCAAATG CAGAGATTAA TGCAGAGTTA GGATTATCAG ACGCCTCTGG TGAAGAAGAT 
GCATATGGAA ACTCCAAAAC TCATAAGGAG TTGGAATATC AACTGCCTTT GGTTTCCCAA 
GAGTCAACTT TTACCCGCAT TTTCACAAAT GAATTTCTGC ATCGTAACCC AAGAACTCAG 
ACTATTGATG AGGTAATGGT TTGCCATTGC AAGCCACCTC GAGAAGGCAA GATGGGATGT 
GGGGATGAAT GTCTAAACCG GATGCTTAAC ATCGAATGTG TGCAAGGAAC TTGCCCATGT 
GGGGACCGTT GTTCCAATCA GCAGTTCCAA AAACGCAATT ATTCCAGACT GAAGTGGTTT 
AAATGCGGGA AAAAAGGTTA TGGACTGAAG GCACTTGAGC GTGTAGCTGA AGGCCAGTTT 
ATTATTGAAT ACGTTGGAGA GGTGCTTGAC GTGCATGCAT ACGAGGCACG CCAGAGAGAG 
TATGCTTTGA AGGGTCATAG ACATTTCTAT TTTATGACCT TGAACGGCAG TGAGGTGATA 
GATGCAAGTG CAAAAGGAAA TTTGGGGCGT TTCATTAATC ATAGTTGTGA TCCTAATTGT 
CGGACAGAAA AGTGGATGGT GAATGGAGAA ATCTGTATTG GACTGTTTGC ATTGAGGAAT 
ATCAAGCAGG ATGAAGAATT GACATTTGAT TACAACTATG TAAGGGTTTT TGGTGCTGCT 
GCCAAAAAGT GTTATTGTGG TTCACTTCAC TGTCAAGGAT ATATAGGCGG TGCGGATCCA 
AATAACGGTG AGCTTATAGT TCAAGGTGAA TCAGATGATG AATTTCCAGA ACCTATGATG 
CTTTCTGAAA ATGGTGAAAT TGACGACAGC GTACTCATGC CCAAATGCAT TGATAGTGTT 
AATACAAAGT CTTCTAGGCA TTTAATAACA GACAGGGATG TATTGGACAA GTGTACAACT 
GCTATATGTG CAGATGGTTC TCCAGAGGAA GACAGTTCTA CAAACCCTGC CTCTGCTGTT 
TCCCTGTTGC ACAGTTCAGT TGAAGTGGAA GACTCAAAAA GCAATTTACC ATCTTCTGAT 
CGAATAGAAG AAATTTCTCA ACAAATAGAG GATACAACAA GCAAACCCAT GCCTGCTGAC 
AGCAAAGAAT TGCCGAATTC TACTGATAGT AATAGAGAAT CCAAATCTGA AATGGTTGAA 
GTCGGGAATG ATTTTTCACA ATCACATCTG CTTGTGAAGA CTCCTCGACT AAATGCTTCT 
GTTAAAAAGG GTAAGGTAAG GGCTAATGCT GCAAATGCCC TTACAGCCGA GGTGGCGGCC 
CCTCGATTGC CAGTATCATC TATCAAAAAC AAAAAAGTAG AAGGTTCTTC CAATGGACGG 
TTTGAAGCAG TTCAGGGGAA ACTTAATGAG TTGCTGGATG GAAATGGAGG AATAAGCAAA 
AGAAAAGATG CCACCAAAGG CTACTTGAAA CTTTTGCTTC TCACCGTGGC ATCAGGCGAT 
AGAAGCAATG GTGAAGCAAT TCAGAGTAAC CGAGATCTTT CCATGATCCT TGATGCTCTT 
CTGAAAACAA AATCGAGAGC CGTGCTAAAT GATATTATTA GTAAAAATGG TTTACAGATG 
TTACATAAGA TCATGAAGCA GTATAGGCAA GACTTCAAAA AAATTCCAAT ACTACGAAAG 
CTTCTTAAGG TCTTAGAGTA CCTGGCAGCA GGCAAGATTT TGACTCCTGA ACATATCAAT 
GGTGGTCCTC CTTGTCATGG AATGGAGAGG TTTAGGGATT CAATGCTGTC TCTGACAGAG 
CATGATGATA AACAGGTCCA TCAAATTGCT CGAAGCTTCC GAGACAGATG GATTCCAAGA 
CATGGCAGAA AACATGGCTA TATGGACAGG GATGATAACA GGATGGAATC TCACAGAGGT 
TTCAACTCCA ACAGATTTTC AGTATCACAC AGTCATAGGC ACGAGCAGGG ATTAAGACCT 
AAGGAAGCAA CTGATTGTGG TCAGCAGCCA ATGCTTGTGG CAACAGTAGA CGCGCGTGCC 
CAGGAGGGCT GCTCTACACC ATCTTTGGAT GGAGTTGAGA TCAACGGGGC AAAAAAACGT 
AAGCGCAAAA GCCGATGGGA TCAACCAGCA GAAACAAATT CATATTCTGA TGCTATCATT 
AGTTCTATTA ATGAAAGCCA GAACGTTCAT GAAGAAGTTC CACCCGGATT CTCATGTCCA 
ATACGTTCAT TAAACAGTGC ACTAAACTCT GGTACTCCTG CTTTGCAAAA TGCAAGCCAT 
TCTGGATGCC CCCCAAGTTT AGTTATTGGT CAGCCAAAAG AGAAATTCAA TTCTCGTTTG 
CCTGTCTCAT ATGGATTGCC ATGGTCTGTT GCGCAACAAT ATGGAACTCC TCATGCTGAA 
ATCACAGGGT GTTGGATCAC TGCTCCTGGC ATGCCTTTCA ACCCGTTTCC TCCATTACCC 
CCATATCCAC GGGATAACAA AGACTGTCAA CCTTCCAGTA TGGAAATTGA TCAGCCTGCT 
GAAGTTAAGC AATCGGACGC CACTGGTCCG GTTAATTGCT GCTCAGATGA TATGATTCCC 
AGCACAACTG GTGCTAACTC TGAAGACACG AACCTTCAAT GTGAGGATGC TAAACATGAC 
GCTAAGCGAT TGAAGGGTGA TGATTCTGAT GATTTGGGAA AGAAGTACTT TAGACAGCAG 
AAATGGAATA ATTCAAAAAT ACATCGAACA TGGTTCAAAA GGGATGCATG GAAATGTAAT 
GGGAACAGCT CTAGTGGTGA TATTTGCAGT ATAGATGTAG GAGATGTGTC AAAAGAATCA 
AAAGTTACTA GTTATTCAGA GGAGGATGAA ATCTGTAGAG AGGAGAAAGG TGGAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101503412 ( XM_004502482.3 ) cDNA ORF clone, Cicer arietinum(chickpea) -> XP_004502539.1 Cicer arietinum histone-lysine N-methyltransferase ASHH2 (LOC101503412), transcript variant X1, mRNA.

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CloneID	OCi113901
Clone ID Related Accession (Same CDS sequence)	XM_004502483.2 , XM_004502482.2 , XM_004502482.3 , XM_004502483.3
Accession Version	XM_004502482.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5940bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-13
Organism	Cicer arietinum(chickpea)
Product	histone-lysine N-methyltransferase ASHH2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_021164.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 14, 2018 this sequence version replaced XM_004502482.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cicer arietinum Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTCGT GTGGTGAACC GGTAATCGTT GGTGATCCGG CACAATTGGA GCAGCCTTTG 
TGCTCGGGTT TTCCGGCACA GTTAGTTTCT GTGCAGGAGT CATGTTCAGA AGAACCTTGC 
GATGTTTTAG ACGTATCTGG TATGGCGGAT GGTTTTTTGG GAGAGGGTGA AGTGGGTTGT 
TCTGCAAGCT ATCTTGATGT TATGGAGGGG TACGGAGATG CTTTGACAAA TGAATGTGAA 
AATGATGATA TGTCACAATT GGAGAAAACT GCTGAAGATG GTTGTTGGAA TAGTTTGGGG 
ATTTGTTCTG AGGACATTGA AGTTTCCGGT CTTAAAAGTG GTGGATTGTG CTTAGAAGGA 
AATTTTAAAG ATGAAGGAAA CCTTGACCTG CCGTTGGAGT CCATAACCTC AAATGATTTG 
CAGAAGCATT GGGCTGAACA GGGTGAGCAG AAGAATGGCA AGAACATTAT GGTATTCTCG 
GTAGGAGATG ATTTGACAGT CGTGGAAGGC AAAAACGATG GTGCTGATTT ACTAGCTGGT 
GCTGTCAACT GTGTGCTTGA TGTTGAGCAT TCTGAAGTGC CTTTGGAATC AGATAGCTGG 
CCAAAGGCTG TGGAAAATTG TGATGCTTTA GATGGGATAG AAGCGAATTC TTGCAGGCAG 
ATATCTTCTT CACTAAATTT TGAAGTGCCG TCAGGTGTCT TGTTTATAGA TGCAAAAGTA 
GAGAGCACAA GTGATAAGCT ACACGATCTA AAAAATGGCG AGGATTGTGA CAGTACTTGT 
GATGAAAAGA TAAGAGCCTT TGTTGATAAA GAAATCACTG TTAATTCATA TGTACAAGCG 
TCGTCCTCAC CAGATACAAA AGAAGAGAGC ACAAGTGATG AGCTACATGA CCAGAAAAAT 
GGTCAGGATT ATAAAAGTAG TTGTGAAGAA AAGTTAAGAG CCTTTGTTGA TAAAGAATCA 
ACTGTTAATT CGTATTTACA AGCATCGTCC TTGCCAGATT TCCATAGGAC CCTGAGGACT 
TCACCTGTAA TTGATTCACA ATGTCAGCCT ACCTTGATGG ATCCAGGGGG TGAATTGAAG 
AATGGCATAC TGCAAACAGA AGATAATTTC TGTACATTGA AGGATTGTTC TGCAGATGGA 
AATGCCAACT CTACCATTAG AAAACAATTT TATCCAGAAT CAGGTCAGTC CTCTTTTGTT 
GTTCTCACCA ATAGCTTTCC AAAGGATGCG CCGGATCTCC TTTCCAAGGG CGATGGTGCT 
TCCATCAACA ATGATTGTGC TGTAGACAAT CCTGGACAAA CAAATAATGA TGGAAAAGAA 
GATGTTGAAG TTGATCATAT TACTGAAAAC ATACTGTTAC CTTCTCAAAG GAGTCAAAGA 
ACCAAATTTG GTAGTAAGAC ACAAACCAGG AAGGCTTCTA GAAAATCCAA GAACAAAGCC 
AGTATGACAC ATCGAGGTGG AGGTATGAAT ATGAACTTAG AGGCTGCTAG AAAGAAAAGA 
AGTTGTTTAT GCAAACCGGC TCGTTCTTCT GTCTGGGGAT CGCTTGGGAA TATTGAACAG 
TTTTTTCAGC ACGAAAACGA GCTGGAGGTT AGTGAAGCCA TGTGCCAGAA TGTGGTGAAA 
GCTAGAAGCA AGTTTCAAGG TGGGGAAATG ATTAAGAATG CTACAAGTTC TAGCTCATTA 
AGTTCAGTTC AAAAGGATAA TGTTCCAACT TCAACTACTC GTTTTCGTCT GAAGATCAAG 
TTTGGAAAAG AAAATGCTTT ACGTTGCTCA AACGTCTTGA TTCCGGAGTC TGTTGGTGGC 
TTGGCTTCTG CTTCCTACTT GGAGTCGGAC CCAGGTTCTC GGAAAGTAGC CAGCAATTCT 
GCAGATAAAT TTTCTGAAGC ATTGGCTTTA AGCAATTTAG AATCATTCAG AAATGACCTG 
GACAAGGATT TTGTTGTTCT AAATGGACAA ATTGTAAATA GCCAGTTAGA AAATTCTAAA 
ATAACGGAGA AGTCAGATGA GGATGCAGTG GAACCATGTC ATGCAGGTCC TCCAGAGAAA 
GTGGTTGAAG CATTAGTTAA GCCAATTAAT AATTCAGTTA TAGATCCCGG AACGTCACCT 
GATTCAGAAG TCATTGATTC AATTCCTGAC GTCCAGGTTG GAGAAAGACA TGAAGATGTA 
CATTTTTCTG TGTTAGGTTC TTCTAAGGAG TTAAATTCTC ATATGGACGT TACCTTCAGG 
AATAGAGGGA AGAAGAAAGA CAAGCTTATT TATTCAGGCA ATTGCATCAC TGAAGATGGA 
TCACAAGGTC AACCAGGGAA TAATAGAGCT AAGCATTCGA AGAATCACAG ACGTAAGAGA 
ACCTCCAGTG ATGTGGTTAG CTCTTTAGAA TTGCCTATTT CCACTGAGCT AAGCAAATCT 
GTAAAGAGTA AAGAATTGTC TGCAGAATCA TCGCCCCTTT CTGGAGATAT TGTACTTGGA 
GGCCCTATGG AGTCTTTGAA AGTTAAAAGT CATGCGATAG TCAAGACAGC TGGCAAATCA 
TCTGCTGACC ATGGTTTTAC TGAACTCCAG GGTTCAAAAA ATTTACCGTC TTCTACAAGA 
CCTTGGGGTC GTAAACTACC TAAAAGTCTT GAACCTAGAA AAGTTAGCAA GGGTAAATCC 
AAAGCTTCTG ACTCGACAAG CAGGAAAAAG CCTACTTCTA GGCCCAAGGA GAGACAGAAA 
GAGCCAATTA ACAGAAGTGA AGTAAAGGGA AAAGATGTCT CTCTCAAAGT TACATGTGAA 
GTGGAAGATC ACCCACATCC AGCAGATATA GTTGGAAATC ATAAGTTGGC TGGTGTTGGA 
AAAATTAATA CCGGTGACAA CAGAGTACCA GTCAGTGTGT CTAATTTGGA CGTGATGCCT 
GGTTTTGGTT TGGAAGAACA GCAGCAGTCA CCTCGTAATG CATGGGTGAG TTGTGATGAC 
TGTCACAAGT GGCGGCGCAT TCCAGCTTTG CTTGCAGACC AGATTGATGA AACTAACTGC 
ACCTGGACTT GCAAGGACAG CAGTGATAAA GCCTATGCTG ATTGTGCTAT CCCTCAAGAA 
AAGTCAAATG CAGAGATTAA TGCAGAGTTA GGATTATCAG ACGCCTCTGG TGAAGAAGAT 
GCATATGGAA ACTCCAAAAC TCATAAGGAG TTGGAATATC AACTGCCTTT GGTTTCCCAA 
GAGTCAACTT TTACCCGCAT TTTCACAAAT GAATTTCTGC ATCGTAACCC AAGAACTCAG 
ACTATTGATG AGGTAATGGT TTGCCATTGC AAGCCACCTC GAGAAGGCAA GATGGGATGT 
GGGGATGAAT GTCTAAACCG GATGCTTAAC ATCGAATGTG TGCAAGGAAC TTGCCCATGT 
GGGGACCGTT GTTCCAATCA GCAGTTCCAA AAACGCAATT ATTCCAGACT GAAGTGGTTT 
AAATGCGGGA AAAAAGGTTA TGGACTGAAG GCACTTGAGC GTGTAGCTGA AGGCCAGTTT 
ATTATTGAAT ACGTTGGAGA GGTGCTTGAC GTGCATGCAT ACGAGGCACG CCAGAGAGAG 
TATGCTTTGA AGGGTCATAG ACATTTCTAT TTTATGACCT TGAACGGCAG TGAGGTGATA 
GATGCAAGTG CAAAAGGAAA TTTGGGGCGT TTCATTAATC ATAGTTGTGA TCCTAATTGT 
CGGACAGAAA AGTGGATGGT GAATGGAGAA ATCTGTATTG GACTGTTTGC ATTGAGGAAT 
ATCAAGCAGG ATGAAGAATT GACATTTGAT TACAACTATG TAAGGGTTTT TGGTGCTGCT 
GCCAAAAAGT GTTATTGTGG TTCACTTCAC TGTCAAGGAT ATATAGGCGG TGCGGATCCA 
AATAACGGTG AGCTTATAGT TCAAGGTGAA TCAGATGATG AATTTCCAGA ACCTATGATG 
CTTTCTGAAA ATGGTGAAAT TGACGACAGC GTACTCATGC CCAAATGCAT TGATAGTGTT 
AATACAAAGT CTTCTAGGCA TTTAATAACA GACAGGGATG TATTGGACAA GTGTACAACT 
GCTATATGTG CAGATGGTTC TCCAGAGGAA GACAGTTCTA CAAACCCTGC CTCTGCTGTT 
TCCCTGTTGC ACAGTTCAGT TGAAGTGGAA GACTCAAAAA GCAATTTACC ATCTTCTGAT 
CGAATAGAAG AAATTTCTCA ACAAATAGAG GATACAACAA GCAAACCCAT GCCTGCTGAC 
AGCAAAGAAT TGCCGAATTC TACTGATAGT AATAGAGAAT CCAAATCTGA AATGGTTGAA 
GTCGGGAATG ATTTTTCACA ATCACATCTG CTTGTGAAGA CTCCTCGACT AAATGCTTCT 
GTTAAAAAGG GTAAGGTAAG GGCTAATGCT GCAAATGCCC TTACAGCCGA GGTGGCGGCC 
CCTCGATTGC CAGTATCATC TATCAAAAAC AAAAAAGTAG AAGGTTCTTC CAATGGACGG 
TTTGAAGCAG TTCAGGGGAA ACTTAATGAG TTGCTGGATG GAAATGGAGG AATAAGCAAA 
AGAAAAGATG CCACCAAAGG CTACTTGAAA CTTTTGCTTC TCACCGTGGC ATCAGGCGAT 
AGAAGCAATG GTGAAGCAAT TCAGAGTAAC CGAGATCTTT CCATGATCCT TGATGCTCTT 
CTGAAAACAA AATCGAGAGC CGTGCTAAAT GATATTATTA GTAAAAATGG TTTACAGATG 
TTACATAAGA TCATGAAGCA GTATAGGCAA GACTTCAAAA AAATTCCAAT ACTACGAAAG 
CTTCTTAAGG TCTTAGAGTA CCTGGCAGCA GGCAAGATTT TGACTCCTGA ACATATCAAT 
GGTGGTCCTC CTTGTCATGG AATGGAGAGG TTTAGGGATT CAATGCTGTC TCTGACAGAG 
CATGATGATA AACAGGTCCA TCAAATTGCT CGAAGCTTCC GAGACAGATG GATTCCAAGA 
CATGGCAGAA AACATGGCTA TATGGACAGG GATGATAACA GGATGGAATC TCACAGAGGT 
TTCAACTCCA ACAGATTTTC AGTATCACAC AGTCATAGGC ACGAGCAGGG ATTAAGACCT 
AAGGAAGCAA CTGATTGTGG TCAGCAGCCA ATGCTTGTGG CAACAGTAGA CGCGCGTGCC 
CAGGAGGGCT GCTCTACACC ATCTTTGGAT GGAGTTGAGA TCAACGGGGC AAAAAAACGT 
AAGCGCAAAA GCCGATGGGA TCAACCAGCA GAAACAAATT CATATTCTGA TGCTATCATT 
AGTTCTATTA ATGAAAGCCA GAACGTTCAT GAAGAAGTTC CACCCGGATT CTCATGTCCA 
ATACGTTCAT TAAACAGTGC ACTAAACTCT GGTACTCCTG CTTTGCAAAA TGCAAGCCAT 
TCTGGATGCC CCCCAAGTTT AGTTATTGGT CAGCCAAAAG AGAAATTCAA TTCTCGTTTG 
CCTGTCTCAT ATGGATTGCC ATGGTCTGTT GCGCAACAAT ATGGAACTCC TCATGCTGAA 
ATCACAGGGT GTTGGATCAC TGCTCCTGGC ATGCCTTTCA ACCCGTTTCC TCCATTACCC 
CCATATCCAC GGGATAACAA AGACTGTCAA CCTTCCAGTA TGGAAATTGA TCAGCCTGCT 
GAAGTTAAGC AATCGGACGC CACTGGTCCG GTTAATTGCT GCTCAGATGA TATGATTCCC 
AGCACAACTG GTGCTAACTC TGAAGACACG AACCTTCAAT GTGAGGATGC TAAACATGAC 
GCTAAGCGAT TGAAGGGTGA TGATTCTGAT GATTTGGGAA AGAAGTACTT TAGACAGCAG 
AAATGGAATA ATTCAAAAAT ACATCGAACA TGGTTCAAAA GGGATGCATG GAAATGTAAT 
GGGAACAGCT CTAGTGGTGA TATTTGCAGT ATAGATGTAG GAGATGTGTC AAAAGAATCA 
AAAGTTACTA GTTATTCAGA GGAGGATGAA ATCTGTAGAG AGGAGAAAGG TGGAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004502539.1
CDS	372..6311
Translation	MGSCGEPVIVGDPAQLEQPLCSGFPAQLVSVQESCSEEPCDVLDVSGMADGFLGEGEVGCSASYLDVMEGYGDALTNECENDDMSQLEKTAEDGCWNSLGICSEDIEVSGLKSGGLCLEGNFKDEGNLDLPLESITSNDLQKHWAEQGEQKNGKNIMVFSVGDDLTVVEGKNDGADLLAGAVNCVLDVEHSEVPLESDSWPKAVENCDALDGIEANSCRQISSSLNFEVPSGVLFIDAKVESTSDKLHDLKNGEDCDSTCDEKIRAFVDKEITVNSYVQASSSPDTKEESTSDELHDQKNGQDYKSSCEEKLRAFVDKESTVNSYLQASSLPDFHRTLRTSPVIDSQCQPTLMDPGGELKNGILQTEDNFCTLKDCSADGNANSTIRKQFYPESGQSSFVVLTNSFPKDAPDLLSKGDGASINNDCAVDNPGQTNNDGKEDVEVDHITENILLPSQRSQRTKFGSKTQTRKASRKSKNKASMTHRGGGMNMNLEAARKKRSCLCKPARSSVWGSLGNIEQFFQHENELEVSEAMCQNVVKARSKFQGGEMIKNATSSSSLSSVQKDNVPTSTTRFRLKIKFGKENALRCSNVLIPESVGGLASASYLESDPGSRKVASNSADKFSEALALSNLESFRNDLDKDFVVLNGQIVNSQLENSKITEKSDEDAVEPCHAGPPEKVVEALVKPINNSVIDPGTSPDSEVIDSIPDVQVGERHEDVHFSVLGSSKELNSHMDVTFRNRGKKKDKLIYSGNCITEDGSQGQPGNNRAKHSKNHRRKRTSSDVVSSLELPISTELSKSVKSKELSAESSPLSGDIVLGGPMESLKVKSHAIVKTAGKSSADHGFTELQGSKNLPSSTRPWGRKLPKSLEPRKVSKGKSKASDSTSRKKPTSRPKERQKEPINRSEVKGKDVSLKVTCEVEDHPHPADIVGNHKLAGVGKINTGDNRVPVSVSNLDVMPGFGLEEQQQSPRNAWVSCDDCHKWRRIPALLADQIDETNCTWTCKDSSDKAYADCAIPQEKSNAEINAELGLSDASGEEDAYGNSKTHKELEYQLPLVSQESTFTRIFTNEFLHRNPRTQTIDEVMVCHCKPPREGKMGCGDECLNRMLNIECVQGTCPCGDRCSNQQFQKRNYSRLKWFKCGKKGYGLKALERVAEGQFIIEYVGEVLDVHAYEARQREYALKGHRHFYFMTLNGSEVIDASAKGNLGRFINHSCDPNCRTEKWMVNGEICIGLFALRNIKQDEELTFDYNYVRVFGAAAKKCYCGSLHCQGYIGGADPNNGELIVQGESDDEFPEPMMLSENGEIDDSVLMPKCIDSVNTKSSRHLITDRDVLDKCTTAICADGSPEEDSSTNPASAVSLLHSSVEVEDSKSNLPSSDRIEEISQQIEDTTSKPMPADSKELPNSTDSNRESKSEMVEVGNDFSQSHLLVKTPRLNASVKKGKVRANAANALTAEVAAPRLPVSSIKNKKVEGSSNGRFEAVQGKLNELLDGNGGISKRKDATKGYLKLLLLTVASGDRSNGEAIQSNRDLSMILDALLKTKSRAVLNDIISKNGLQMLHKIMKQYRQDFKKIPILRKLLKVLEYLAAGKILTPEHINGGPPCHGMERFRDSMLSLTEHDDKQVHQIARSFRDRWIPRHGRKHGYMDRDDNRMESHRGFNSNRFSVSHSHRHEQGLRPKEATDCGQQPMLVATVDARAQEGCSTPSLDGVEINGAKKRKRKSRWDQPAETNSYSDAIISSINESQNVHEEVPPGFSCPIRSLNSALNSGTPALQNASHSGCPPSLVIGQPKEKFNSRLPVSYGLPWSVAQQYGTPHAEITGCWITAPGMPFNPFPPLPPYPRDNKDCQPSSMEIDQPAEVKQSDATGPVNCCSDDMIPSTTGANSEDTNLQCEDAKHDAKRLKGDDSDDLGKKYFRQQKWNNSKIHRTWFKRDAWKCNGNSSSGDICSIDVGDVSKESKVTSYSEEDEICREEKGGK

Target ORF information:

RefSeq Version	XM_004502482.3
Organism	Cicer arietinum(chickpea)
Definition	Cicer arietinum histone-lysine N-methyltransferase ASHH2 (LOC101503412), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004502482.3

ORF Insert Sequence:

ATGGGCTCGT GTGGTGAACC GGTAATCGTT GGTGATCCGG CACAATTGGA GCAGCCTTTG 
TGCTCGGGTT TTCCGGCACA GTTAGTTTCT GTGCAGGAGT CATGTTCAGA AGAACCTTGC 
GATGTTTTAG ACGTATCTGG TATGGCGGAT GGTTTTTTGG GAGAGGGTGA AGTGGGTTGT 
TCTGCAAGCT ATCTTGATGT TATGGAGGGG TACGGAGATG CTTTGACAAA TGAATGTGAA 
AATGATGATA TGTCACAATT GGAGAAAACT GCTGAAGATG GTTGTTGGAA TAGTTTGGGG 
ATTTGTTCTG AGGACATTGA AGTTTCCGGT CTTAAAAGTG GTGGATTGTG CTTAGAAGGA 
AATTTTAAAG ATGAAGGAAA CCTTGACCTG CCGTTGGAGT CCATAACCTC AAATGATTTG 
CAGAAGCATT GGGCTGAACA GGGTGAGCAG AAGAATGGCA AGAACATTAT GGTATTCTCG 
GTAGGAGATG ATTTGACAGT CGTGGAAGGC AAAAACGATG GTGCTGATTT ACTAGCTGGT 
GCTGTCAACT GTGTGCTTGA TGTTGAGCAT TCTGAAGTGC CTTTGGAATC AGATAGCTGG 
CCAAAGGCTG TGGAAAATTG TGATGCTTTA GATGGGATAG AAGCGAATTC TTGCAGGCAG 
ATATCTTCTT CACTAAATTT TGAAGTGCCG TCAGGTGTCT TGTTTATAGA TGCAAAAGTA 
GAGAGCACAA GTGATAAGCT ACACGATCTA AAAAATGGCG AGGATTGTGA CAGTACTTGT 
GATGAAAAGA TAAGAGCCTT TGTTGATAAA GAAATCACTG TTAATTCATA TGTACAAGCG 
TCGTCCTCAC CAGATACAAA AGAAGAGAGC ACAAGTGATG AGCTACATGA CCAGAAAAAT 
GGTCAGGATT ATAAAAGTAG TTGTGAAGAA AAGTTAAGAG CCTTTGTTGA TAAAGAATCA 
ACTGTTAATT CGTATTTACA AGCATCGTCC TTGCCAGATT TCCATAGGAC CCTGAGGACT 
TCACCTGTAA TTGATTCACA ATGTCAGCCT ACCTTGATGG ATCCAGGGGG TGAATTGAAG 
AATGGCATAC TGCAAACAGA AGATAATTTC TGTACATTGA AGGATTGTTC TGCAGATGGA 
AATGCCAACT CTACCATTAG AAAACAATTT TATCCAGAAT CAGGTCAGTC CTCTTTTGTT 
GTTCTCACCA ATAGCTTTCC AAAGGATGCG CCGGATCTCC TTTCCAAGGG CGATGGTGCT 
TCCATCAACA ATGATTGTGC TGTAGACAAT CCTGGACAAA CAAATAATGA TGGAAAAGAA 
GATGTTGAAG TTGATCATAT TACTGAAAAC ATACTGTTAC CTTCTCAAAG GAGTCAAAGA 
ACCAAATTTG GTAGTAAGAC ACAAACCAGG AAGGCTTCTA GAAAATCCAA GAACAAAGCC 
AGTATGACAC ATCGAGGTGG AGGTATGAAT ATGAACTTAG AGGCTGCTAG AAAGAAAAGA 
AGTTGTTTAT GCAAACCGGC TCGTTCTTCT GTCTGGGGAT CGCTTGGGAA TATTGAACAG 
TTTTTTCAGC ACGAAAACGA GCTGGAGGTT AGTGAAGCCA TGTGCCAGAA TGTGGTGAAA 
GCTAGAAGCA AGTTTCAAGG TGGGGAAATG ATTAAGAATG CTACAAGTTC TAGCTCATTA 
AGTTCAGTTC AAAAGGATAA TGTTCCAACT TCAACTACTC GTTTTCGTCT GAAGATCAAG 
TTTGGAAAAG AAAATGCTTT ACGTTGCTCA AACGTCTTGA TTCCGGAGTC TGTTGGTGGC 
TTGGCTTCTG CTTCCTACTT GGAGTCGGAC CCAGGTTCTC GGAAAGTAGC CAGCAATTCT 
GCAGATAAAT TTTCTGAAGC ATTGGCTTTA AGCAATTTAG AATCATTCAG AAATGACCTG 
GACAAGGATT TTGTTGTTCT AAATGGACAA ATTGTAAATA GCCAGTTAGA AAATTCTAAA 
ATAACGGAGA AGTCAGATGA GGATGCAGTG GAACCATGTC ATGCAGGTCC TCCAGAGAAA 
GTGGTTGAAG CATTAGTTAA GCCAATTAAT AATTCAGTTA TAGATCCCGG AACGTCACCT 
GATTCAGAAG TCATTGATTC AATTCCTGAC GTCCAGGTTG GAGAAAGACA TGAAGATGTA 
CATTTTTCTG TGTTAGGTTC TTCTAAGGAG TTAAATTCTC ATATGGACGT TACCTTCAGG 
AATAGAGGGA AGAAGAAAGA CAAGCTTATT TATTCAGGCA ATTGCATCAC TGAAGATGGA 
TCACAAGGTC AACCAGGGAA TAATAGAGCT AAGCATTCGA AGAATCACAG ACGTAAGAGA 
ACCTCCAGTG ATGTGGTTAG CTCTTTAGAA TTGCCTATTT CCACTGAGCT AAGCAAATCT 
GTAAAGAGTA AAGAATTGTC TGCAGAATCA TCGCCCCTTT CTGGAGATAT TGTACTTGGA 
GGCCCTATGG AGTCTTTGAA AGTTAAAAGT CATGCGATAG TCAAGACAGC TGGCAAATCA 
TCTGCTGACC ATGGTTTTAC TGAACTCCAG GGTTCAAAAA ATTTACCGTC TTCTACAAGA 
CCTTGGGGTC GTAAACTACC TAAAAGTCTT GAACCTAGAA AAGTTAGCAA GGGTAAATCC 
AAAGCTTCTG ACTCGACAAG CAGGAAAAAG CCTACTTCTA GGCCCAAGGA GAGACAGAAA 
GAGCCAATTA ACAGAAGTGA AGTAAAGGGA AAAGATGTCT CTCTCAAAGT TACATGTGAA 
GTGGAAGATC ACCCACATCC AGCAGATATA GTTGGAAATC ATAAGTTGGC TGGTGTTGGA 
AAAATTAATA CCGGTGACAA CAGAGTACCA GTCAGTGTGT CTAATTTGGA CGTGATGCCT 
GGTTTTGGTT TGGAAGAACA GCAGCAGTCA CCTCGTAATG CATGGGTGAG TTGTGATGAC 
TGTCACAAGT GGCGGCGCAT TCCAGCTTTG CTTGCAGACC AGATTGATGA AACTAACTGC 
ACCTGGACTT GCAAGGACAG CAGTGATAAA GCCTATGCTG ATTGTGCTAT CCCTCAAGAA 
AAGTCAAATG CAGAGATTAA TGCAGAGTTA GGATTATCAG ACGCCTCTGG TGAAGAAGAT 
GCATATGGAA ACTCCAAAAC TCATAAGGAG TTGGAATATC AACTGCCTTT GGTTTCCCAA 
GAGTCAACTT TTACCCGCAT TTTCACAAAT GAATTTCTGC ATCGTAACCC AAGAACTCAG 
ACTATTGATG AGGTAATGGT TTGCCATTGC AAGCCACCTC GAGAAGGCAA GATGGGATGT 
GGGGATGAAT GTCTAAACCG GATGCTTAAC ATCGAATGTG TGCAAGGAAC TTGCCCATGT 
GGGGACCGTT GTTCCAATCA GCAGTTCCAA AAACGCAATT ATTCCAGACT GAAGTGGTTT 
AAATGCGGGA AAAAAGGTTA TGGACTGAAG GCACTTGAGC GTGTAGCTGA AGGCCAGTTT 
ATTATTGAAT ACGTTGGAGA GGTGCTTGAC GTGCATGCAT ACGAGGCACG CCAGAGAGAG 
TATGCTTTGA AGGGTCATAG ACATTTCTAT TTTATGACCT TGAACGGCAG TGAGGTGATA 
GATGCAAGTG CAAAAGGAAA TTTGGGGCGT TTCATTAATC ATAGTTGTGA TCCTAATTGT 
CGGACAGAAA AGTGGATGGT GAATGGAGAA ATCTGTATTG GACTGTTTGC ATTGAGGAAT 
ATCAAGCAGG ATGAAGAATT GACATTTGAT TACAACTATG TAAGGGTTTT TGGTGCTGCT 
GCCAAAAAGT GTTATTGTGG TTCACTTCAC TGTCAAGGAT ATATAGGCGG TGCGGATCCA 
AATAACGGTG AGCTTATAGT TCAAGGTGAA TCAGATGATG AATTTCCAGA ACCTATGATG 
CTTTCTGAAA ATGGTGAAAT TGACGACAGC GTACTCATGC CCAAATGCAT TGATAGTGTT 
AATACAAAGT CTTCTAGGCA TTTAATAACA GACAGGGATG TATTGGACAA GTGTACAACT 
GCTATATGTG CAGATGGTTC TCCAGAGGAA GACAGTTCTA CAAACCCTGC CTCTGCTGTT 
TCCCTGTTGC ACAGTTCAGT TGAAGTGGAA GACTCAAAAA GCAATTTACC ATCTTCTGAT 
CGAATAGAAG AAATTTCTCA ACAAATAGAG GATACAACAA GCAAACCCAT GCCTGCTGAC 
AGCAAAGAAT TGCCGAATTC TACTGATAGT AATAGAGAAT CCAAATCTGA AATGGTTGAA 
GTCGGGAATG ATTTTTCACA ATCACATCTG CTTGTGAAGA CTCCTCGACT AAATGCTTCT 
GTTAAAAAGG GTAAGGTAAG GGCTAATGCT GCAAATGCCC TTACAGCCGA GGTGGCGGCC 
CCTCGATTGC CAGTATCATC TATCAAAAAC AAAAAAGTAG AAGGTTCTTC CAATGGACGG 
TTTGAAGCAG TTCAGGGGAA ACTTAATGAG TTGCTGGATG GAAATGGAGG AATAAGCAAA 
AGAAAAGATG CCACCAAAGG CTACTTGAAA CTTTTGCTTC TCACCGTGGC ATCAGGCGAT 
AGAAGCAATG GTGAAGCAAT TCAGAGTAAC CGAGATCTTT CCATGATCCT TGATGCTCTT 
CTGAAAACAA AATCGAGAGC CGTGCTAAAT GATATTATTA GTAAAAATGG TTTACAGATG 
TTACATAAGA TCATGAAGCA GTATAGGCAA GACTTCAAAA AAATTCCAAT ACTACGAAAG 
CTTCTTAAGG TCTTAGAGTA CCTGGCAGCA GGCAAGATTT TGACTCCTGA ACATATCAAT 
GGTGGTCCTC CTTGTCATGG AATGGAGAGG TTTAGGGATT CAATGCTGTC TCTGACAGAG 
CATGATGATA AACAGGTCCA TCAAATTGCT CGAAGCTTCC GAGACAGATG GATTCCAAGA 
CATGGCAGAA AACATGGCTA TATGGACAGG GATGATAACA GGATGGAATC TCACAGAGGT 
TTCAACTCCA ACAGATTTTC AGTATCACAC AGTCATAGGC ACGAGCAGGG ATTAAGACCT 
AAGGAAGCAA CTGATTGTGG TCAGCAGCCA ATGCTTGTGG CAACAGTAGA CGCGCGTGCC 
CAGGAGGGCT GCTCTACACC ATCTTTGGAT GGAGTTGAGA TCAACGGGGC AAAAAAACGT 
AAGCGCAAAA GCCGATGGGA TCAACCAGCA GAAACAAATT CATATTCTGA TGCTATCATT 
AGTTCTATTA ATGAAAGCCA GAACGTTCAT GAAGAAGTTC CACCCGGATT CTCATGTCCA 
ATACGTTCAT TAAACAGTGC ACTAAACTCT GGTACTCCTG CTTTGCAAAA TGCAAGCCAT 
TCTGGATGCC CCCCAAGTTT AGTTATTGGT CAGCCAAAAG AGAAATTCAA TTCTCGTTTG 
CCTGTCTCAT ATGGATTGCC ATGGTCTGTT GCGCAACAAT ATGGAACTCC TCATGCTGAA 
ATCACAGGGT GTTGGATCAC TGCTCCTGGC ATGCCTTTCA ACCCGTTTCC TCCATTACCC 
CCATATCCAC GGGATAACAA AGACTGTCAA CCTTCCAGTA TGGAAATTGA TCAGCCTGCT 
GAAGTTAAGC AATCGGACGC CACTGGTCCG GTTAATTGCT GCTCAGATGA TATGATTCCC 
AGCACAACTG GTGCTAACTC TGAAGACACG AACCTTCAAT GTGAGGATGC TAAACATGAC 
GCTAAGCGAT TGAAGGGTGA TGATTCTGAT GATTTGGGAA AGAAGTACTT TAGACAGCAG 
AAATGGAATA ATTCAAAAAT ACATCGAACA TGGTTCAAAA GGGATGCATG GAAATGTAAT 
GGGAACAGCT CTAGTGGTGA TATTTGCAGT ATAGATGTAG GAGATGTGTC AAAAGAATCA 
AAAGTTACTA GTTATTCAGA GGAGGATGAA ATCTGTAGAG AGGAGAAAGG TGGAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101503412 ( XM_004502483.3 ) cDNA ORF clone, Cicer arietinum(chickpea) -> XP_004502540.1 Cicer arietinum histone-lysine N-methyltransferase ASHH2 (LOC101503412), transcript variant X2, mRNA.

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CloneID	OCi113901
Clone ID Related Accession (Same CDS sequence)	XM_004502483.2 , XM_004502482.2 , XM_004502482.3 , XM_004502483.3
Accession Version	XM_004502483.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5940bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-13
Organism	Cicer arietinum(chickpea)
Product	histone-lysine N-methyltransferase ASHH2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_021164.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 14, 2018 this sequence version replaced XM_004502483.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cicer arietinum Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTCGT GTGGTGAACC GGTAATCGTT GGTGATCCGG CACAATTGGA GCAGCCTTTG 
TGCTCGGGTT TTCCGGCACA GTTAGTTTCT GTGCAGGAGT CATGTTCAGA AGAACCTTGC 
GATGTTTTAG ACGTATCTGG TATGGCGGAT GGTTTTTTGG GAGAGGGTGA AGTGGGTTGT 
TCTGCAAGCT ATCTTGATGT TATGGAGGGG TACGGAGATG CTTTGACAAA TGAATGTGAA 
AATGATGATA TGTCACAATT GGAGAAAACT GCTGAAGATG GTTGTTGGAA TAGTTTGGGG 
ATTTGTTCTG AGGACATTGA AGTTTCCGGT CTTAAAAGTG GTGGATTGTG CTTAGAAGGA 
AATTTTAAAG ATGAAGGAAA CCTTGACCTG CCGTTGGAGT CCATAACCTC AAATGATTTG 
CAGAAGCATT GGGCTGAACA GGGTGAGCAG AAGAATGGCA AGAACATTAT GGTATTCTCG 
GTAGGAGATG ATTTGACAGT CGTGGAAGGC AAAAACGATG GTGCTGATTT ACTAGCTGGT 
GCTGTCAACT GTGTGCTTGA TGTTGAGCAT TCTGAAGTGC CTTTGGAATC AGATAGCTGG 
CCAAAGGCTG TGGAAAATTG TGATGCTTTA GATGGGATAG AAGCGAATTC TTGCAGGCAG 
ATATCTTCTT CACTAAATTT TGAAGTGCCG TCAGGTGTCT TGTTTATAGA TGCAAAAGTA 
GAGAGCACAA GTGATAAGCT ACACGATCTA AAAAATGGCG AGGATTGTGA CAGTACTTGT 
GATGAAAAGA TAAGAGCCTT TGTTGATAAA GAAATCACTG TTAATTCATA TGTACAAGCG 
TCGTCCTCAC CAGATACAAA AGAAGAGAGC ACAAGTGATG AGCTACATGA CCAGAAAAAT 
GGTCAGGATT ATAAAAGTAG TTGTGAAGAA AAGTTAAGAG CCTTTGTTGA TAAAGAATCA 
ACTGTTAATT CGTATTTACA AGCATCGTCC TTGCCAGATT TCCATAGGAC CCTGAGGACT 
TCACCTGTAA TTGATTCACA ATGTCAGCCT ACCTTGATGG ATCCAGGGGG TGAATTGAAG 
AATGGCATAC TGCAAACAGA AGATAATTTC TGTACATTGA AGGATTGTTC TGCAGATGGA 
AATGCCAACT CTACCATTAG AAAACAATTT TATCCAGAAT CAGGTCAGTC CTCTTTTGTT 
GTTCTCACCA ATAGCTTTCC AAAGGATGCG CCGGATCTCC TTTCCAAGGG CGATGGTGCT 
TCCATCAACA ATGATTGTGC TGTAGACAAT CCTGGACAAA CAAATAATGA TGGAAAAGAA 
GATGTTGAAG TTGATCATAT TACTGAAAAC ATACTGTTAC CTTCTCAAAG GAGTCAAAGA 
ACCAAATTTG GTAGTAAGAC ACAAACCAGG AAGGCTTCTA GAAAATCCAA GAACAAAGCC 
AGTATGACAC ATCGAGGTGG AGGTATGAAT ATGAACTTAG AGGCTGCTAG AAAGAAAAGA 
AGTTGTTTAT GCAAACCGGC TCGTTCTTCT GTCTGGGGAT CGCTTGGGAA TATTGAACAG 
TTTTTTCAGC ACGAAAACGA GCTGGAGGTT AGTGAAGCCA TGTGCCAGAA TGTGGTGAAA 
GCTAGAAGCA AGTTTCAAGG TGGGGAAATG ATTAAGAATG CTACAAGTTC TAGCTCATTA 
AGTTCAGTTC AAAAGGATAA TGTTCCAACT TCAACTACTC GTTTTCGTCT GAAGATCAAG 
TTTGGAAAAG AAAATGCTTT ACGTTGCTCA AACGTCTTGA TTCCGGAGTC TGTTGGTGGC 
TTGGCTTCTG CTTCCTACTT GGAGTCGGAC CCAGGTTCTC GGAAAGTAGC CAGCAATTCT 
GCAGATAAAT TTTCTGAAGC ATTGGCTTTA AGCAATTTAG AATCATTCAG AAATGACCTG 
GACAAGGATT TTGTTGTTCT AAATGGACAA ATTGTAAATA GCCAGTTAGA AAATTCTAAA 
ATAACGGAGA AGTCAGATGA GGATGCAGTG GAACCATGTC ATGCAGGTCC TCCAGAGAAA 
GTGGTTGAAG CATTAGTTAA GCCAATTAAT AATTCAGTTA TAGATCCCGG AACGTCACCT 
GATTCAGAAG TCATTGATTC AATTCCTGAC GTCCAGGTTG GAGAAAGACA TGAAGATGTA 
CATTTTTCTG TGTTAGGTTC TTCTAAGGAG TTAAATTCTC ATATGGACGT TACCTTCAGG 
AATAGAGGGA AGAAGAAAGA CAAGCTTATT TATTCAGGCA ATTGCATCAC TGAAGATGGA 
TCACAAGGTC AACCAGGGAA TAATAGAGCT AAGCATTCGA AGAATCACAG ACGTAAGAGA 
ACCTCCAGTG ATGTGGTTAG CTCTTTAGAA TTGCCTATTT CCACTGAGCT AAGCAAATCT 
GTAAAGAGTA AAGAATTGTC TGCAGAATCA TCGCCCCTTT CTGGAGATAT TGTACTTGGA 
GGCCCTATGG AGTCTTTGAA AGTTAAAAGT CATGCGATAG TCAAGACAGC TGGCAAATCA 
TCTGCTGACC ATGGTTTTAC TGAACTCCAG GGTTCAAAAA ATTTACCGTC TTCTACAAGA 
CCTTGGGGTC GTAAACTACC TAAAAGTCTT GAACCTAGAA AAGTTAGCAA GGGTAAATCC 
AAAGCTTCTG ACTCGACAAG CAGGAAAAAG CCTACTTCTA GGCCCAAGGA GAGACAGAAA 
GAGCCAATTA ACAGAAGTGA AGTAAAGGGA AAAGATGTCT CTCTCAAAGT TACATGTGAA 
GTGGAAGATC ACCCACATCC AGCAGATATA GTTGGAAATC ATAAGTTGGC TGGTGTTGGA 
AAAATTAATA CCGGTGACAA CAGAGTACCA GTCAGTGTGT CTAATTTGGA CGTGATGCCT 
GGTTTTGGTT TGGAAGAACA GCAGCAGTCA CCTCGTAATG CATGGGTGAG TTGTGATGAC 
TGTCACAAGT GGCGGCGCAT TCCAGCTTTG CTTGCAGACC AGATTGATGA AACTAACTGC 
ACCTGGACTT GCAAGGACAG CAGTGATAAA GCCTATGCTG ATTGTGCTAT CCCTCAAGAA 
AAGTCAAATG CAGAGATTAA TGCAGAGTTA GGATTATCAG ACGCCTCTGG TGAAGAAGAT 
GCATATGGAA ACTCCAAAAC TCATAAGGAG TTGGAATATC AACTGCCTTT GGTTTCCCAA 
GAGTCAACTT TTACCCGCAT TTTCACAAAT GAATTTCTGC ATCGTAACCC AAGAACTCAG 
ACTATTGATG AGGTAATGGT TTGCCATTGC AAGCCACCTC GAGAAGGCAA GATGGGATGT 
GGGGATGAAT GTCTAAACCG GATGCTTAAC ATCGAATGTG TGCAAGGAAC TTGCCCATGT 
GGGGACCGTT GTTCCAATCA GCAGTTCCAA AAACGCAATT ATTCCAGACT GAAGTGGTTT 
AAATGCGGGA AAAAAGGTTA TGGACTGAAG GCACTTGAGC GTGTAGCTGA AGGCCAGTTT 
ATTATTGAAT ACGTTGGAGA GGTGCTTGAC GTGCATGCAT ACGAGGCACG CCAGAGAGAG 
TATGCTTTGA AGGGTCATAG ACATTTCTAT TTTATGACCT TGAACGGCAG TGAGGTGATA 
GATGCAAGTG CAAAAGGAAA TTTGGGGCGT TTCATTAATC ATAGTTGTGA TCCTAATTGT 
CGGACAGAAA AGTGGATGGT GAATGGAGAA ATCTGTATTG GACTGTTTGC ATTGAGGAAT 
ATCAAGCAGG ATGAAGAATT GACATTTGAT TACAACTATG TAAGGGTTTT TGGTGCTGCT 
GCCAAAAAGT GTTATTGTGG TTCACTTCAC TGTCAAGGAT ATATAGGCGG TGCGGATCCA 
AATAACGGTG AGCTTATAGT TCAAGGTGAA TCAGATGATG AATTTCCAGA ACCTATGATG 
CTTTCTGAAA ATGGTGAAAT TGACGACAGC GTACTCATGC CCAAATGCAT TGATAGTGTT 
AATACAAAGT CTTCTAGGCA TTTAATAACA GACAGGGATG TATTGGACAA GTGTACAACT 
GCTATATGTG CAGATGGTTC TCCAGAGGAA GACAGTTCTA CAAACCCTGC CTCTGCTGTT 
TCCCTGTTGC ACAGTTCAGT TGAAGTGGAA GACTCAAAAA GCAATTTACC ATCTTCTGAT 
CGAATAGAAG AAATTTCTCA ACAAATAGAG GATACAACAA GCAAACCCAT GCCTGCTGAC 
AGCAAAGAAT TGCCGAATTC TACTGATAGT AATAGAGAAT CCAAATCTGA AATGGTTGAA 
GTCGGGAATG ATTTTTCACA ATCACATCTG CTTGTGAAGA CTCCTCGACT AAATGCTTCT 
GTTAAAAAGG GTAAGGTAAG GGCTAATGCT GCAAATGCCC TTACAGCCGA GGTGGCGGCC 
CCTCGATTGC CAGTATCATC TATCAAAAAC AAAAAAGTAG AAGGTTCTTC CAATGGACGG 
TTTGAAGCAG TTCAGGGGAA ACTTAATGAG TTGCTGGATG GAAATGGAGG AATAAGCAAA 
AGAAAAGATG CCACCAAAGG CTACTTGAAA CTTTTGCTTC TCACCGTGGC ATCAGGCGAT 
AGAAGCAATG GTGAAGCAAT TCAGAGTAAC CGAGATCTTT CCATGATCCT TGATGCTCTT 
CTGAAAACAA AATCGAGAGC CGTGCTAAAT GATATTATTA GTAAAAATGG TTTACAGATG 
TTACATAAGA TCATGAAGCA GTATAGGCAA GACTTCAAAA AAATTCCAAT ACTACGAAAG 
CTTCTTAAGG TCTTAGAGTA CCTGGCAGCA GGCAAGATTT TGACTCCTGA ACATATCAAT 
GGTGGTCCTC CTTGTCATGG AATGGAGAGG TTTAGGGATT CAATGCTGTC TCTGACAGAG 
CATGATGATA AACAGGTCCA TCAAATTGCT CGAAGCTTCC GAGACAGATG GATTCCAAGA 
CATGGCAGAA AACATGGCTA TATGGACAGG GATGATAACA GGATGGAATC TCACAGAGGT 
TTCAACTCCA ACAGATTTTC AGTATCACAC AGTCATAGGC ACGAGCAGGG ATTAAGACCT 
AAGGAAGCAA CTGATTGTGG TCAGCAGCCA ATGCTTGTGG CAACAGTAGA CGCGCGTGCC 
CAGGAGGGCT GCTCTACACC ATCTTTGGAT GGAGTTGAGA TCAACGGGGC AAAAAAACGT 
AAGCGCAAAA GCCGATGGGA TCAACCAGCA GAAACAAATT CATATTCTGA TGCTATCATT 
AGTTCTATTA ATGAAAGCCA GAACGTTCAT GAAGAAGTTC CACCCGGATT CTCATGTCCA 
ATACGTTCAT TAAACAGTGC ACTAAACTCT GGTACTCCTG CTTTGCAAAA TGCAAGCCAT 
TCTGGATGCC CCCCAAGTTT AGTTATTGGT CAGCCAAAAG AGAAATTCAA TTCTCGTTTG 
CCTGTCTCAT ATGGATTGCC ATGGTCTGTT GCGCAACAAT ATGGAACTCC TCATGCTGAA 
ATCACAGGGT GTTGGATCAC TGCTCCTGGC ATGCCTTTCA ACCCGTTTCC TCCATTACCC 
CCATATCCAC GGGATAACAA AGACTGTCAA CCTTCCAGTA TGGAAATTGA TCAGCCTGCT 
GAAGTTAAGC AATCGGACGC CACTGGTCCG GTTAATTGCT GCTCAGATGA TATGATTCCC 
AGCACAACTG GTGCTAACTC TGAAGACACG AACCTTCAAT GTGAGGATGC TAAACATGAC 
GCTAAGCGAT TGAAGGGTGA TGATTCTGAT GATTTGGGAA AGAAGTACTT TAGACAGCAG 
AAATGGAATA ATTCAAAAAT ACATCGAACA TGGTTCAAAA GGGATGCATG GAAATGTAAT 
GGGAACAGCT CTAGTGGTGA TATTTGCAGT ATAGATGTAG GAGATGTGTC AAAAGAATCA 
AAAGTTACTA GTTATTCAGA GGAGGATGAA ATCTGTAGAG AGGAGAAAGG TGGAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004502540.1
CDS	434..6373
Translation	MGSCGEPVIVGDPAQLEQPLCSGFPAQLVSVQESCSEEPCDVLDVSGMADGFLGEGEVGCSASYLDVMEGYGDALTNECENDDMSQLEKTAEDGCWNSLGICSEDIEVSGLKSGGLCLEGNFKDEGNLDLPLESITSNDLQKHWAEQGEQKNGKNIMVFSVGDDLTVVEGKNDGADLLAGAVNCVLDVEHSEVPLESDSWPKAVENCDALDGIEANSCRQISSSLNFEVPSGVLFIDAKVESTSDKLHDLKNGEDCDSTCDEKIRAFVDKEITVNSYVQASSSPDTKEESTSDELHDQKNGQDYKSSCEEKLRAFVDKESTVNSYLQASSLPDFHRTLRTSPVIDSQCQPTLMDPGGELKNGILQTEDNFCTLKDCSADGNANSTIRKQFYPESGQSSFVVLTNSFPKDAPDLLSKGDGASINNDCAVDNPGQTNNDGKEDVEVDHITENILLPSQRSQRTKFGSKTQTRKASRKSKNKASMTHRGGGMNMNLEAARKKRSCLCKPARSSVWGSLGNIEQFFQHENELEVSEAMCQNVVKARSKFQGGEMIKNATSSSSLSSVQKDNVPTSTTRFRLKIKFGKENALRCSNVLIPESVGGLASASYLESDPGSRKVASNSADKFSEALALSNLESFRNDLDKDFVVLNGQIVNSQLENSKITEKSDEDAVEPCHAGPPEKVVEALVKPINNSVIDPGTSPDSEVIDSIPDVQVGERHEDVHFSVLGSSKELNSHMDVTFRNRGKKKDKLIYSGNCITEDGSQGQPGNNRAKHSKNHRRKRTSSDVVSSLELPISTELSKSVKSKELSAESSPLSGDIVLGGPMESLKVKSHAIVKTAGKSSADHGFTELQGSKNLPSSTRPWGRKLPKSLEPRKVSKGKSKASDSTSRKKPTSRPKERQKEPINRSEVKGKDVSLKVTCEVEDHPHPADIVGNHKLAGVGKINTGDNRVPVSVSNLDVMPGFGLEEQQQSPRNAWVSCDDCHKWRRIPALLADQIDETNCTWTCKDSSDKAYADCAIPQEKSNAEINAELGLSDASGEEDAYGNSKTHKELEYQLPLVSQESTFTRIFTNEFLHRNPRTQTIDEVMVCHCKPPREGKMGCGDECLNRMLNIECVQGTCPCGDRCSNQQFQKRNYSRLKWFKCGKKGYGLKALERVAEGQFIIEYVGEVLDVHAYEARQREYALKGHRHFYFMTLNGSEVIDASAKGNLGRFINHSCDPNCRTEKWMVNGEICIGLFALRNIKQDEELTFDYNYVRVFGAAAKKCYCGSLHCQGYIGGADPNNGELIVQGESDDEFPEPMMLSENGEIDDSVLMPKCIDSVNTKSSRHLITDRDVLDKCTTAICADGSPEEDSSTNPASAVSLLHSSVEVEDSKSNLPSSDRIEEISQQIEDTTSKPMPADSKELPNSTDSNRESKSEMVEVGNDFSQSHLLVKTPRLNASVKKGKVRANAANALTAEVAAPRLPVSSIKNKKVEGSSNGRFEAVQGKLNELLDGNGGISKRKDATKGYLKLLLLTVASGDRSNGEAIQSNRDLSMILDALLKTKSRAVLNDIISKNGLQMLHKIMKQYRQDFKKIPILRKLLKVLEYLAAGKILTPEHINGGPPCHGMERFRDSMLSLTEHDDKQVHQIARSFRDRWIPRHGRKHGYMDRDDNRMESHRGFNSNRFSVSHSHRHEQGLRPKEATDCGQQPMLVATVDARAQEGCSTPSLDGVEINGAKKRKRKSRWDQPAETNSYSDAIISSINESQNVHEEVPPGFSCPIRSLNSALNSGTPALQNASHSGCPPSLVIGQPKEKFNSRLPVSYGLPWSVAQQYGTPHAEITGCWITAPGMPFNPFPPLPPYPRDNKDCQPSSMEIDQPAEVKQSDATGPVNCCSDDMIPSTTGANSEDTNLQCEDAKHDAKRLKGDDSDDLGKKYFRQQKWNNSKIHRTWFKRDAWKCNGNSSSGDICSIDVGDVSKESKVTSYSEEDEICREEKGGK

Target ORF information:

RefSeq Version	XM_004502483.3
Organism	Cicer arietinum(chickpea)
Definition	Cicer arietinum histone-lysine N-methyltransferase ASHH2 (LOC101503412), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004502483.3

ORF Insert Sequence:

ATGGGCTCGT GTGGTGAACC GGTAATCGTT GGTGATCCGG CACAATTGGA GCAGCCTTTG 
TGCTCGGGTT TTCCGGCACA GTTAGTTTCT GTGCAGGAGT CATGTTCAGA AGAACCTTGC 
GATGTTTTAG ACGTATCTGG TATGGCGGAT GGTTTTTTGG GAGAGGGTGA AGTGGGTTGT 
TCTGCAAGCT ATCTTGATGT TATGGAGGGG TACGGAGATG CTTTGACAAA TGAATGTGAA 
AATGATGATA TGTCACAATT GGAGAAAACT GCTGAAGATG GTTGTTGGAA TAGTTTGGGG 
ATTTGTTCTG AGGACATTGA AGTTTCCGGT CTTAAAAGTG GTGGATTGTG CTTAGAAGGA 
AATTTTAAAG ATGAAGGAAA CCTTGACCTG CCGTTGGAGT CCATAACCTC AAATGATTTG 
CAGAAGCATT GGGCTGAACA GGGTGAGCAG AAGAATGGCA AGAACATTAT GGTATTCTCG 
GTAGGAGATG ATTTGACAGT CGTGGAAGGC AAAAACGATG GTGCTGATTT ACTAGCTGGT 
GCTGTCAACT GTGTGCTTGA TGTTGAGCAT TCTGAAGTGC CTTTGGAATC AGATAGCTGG 
CCAAAGGCTG TGGAAAATTG TGATGCTTTA GATGGGATAG AAGCGAATTC TTGCAGGCAG 
ATATCTTCTT CACTAAATTT TGAAGTGCCG TCAGGTGTCT TGTTTATAGA TGCAAAAGTA 
GAGAGCACAA GTGATAAGCT ACACGATCTA AAAAATGGCG AGGATTGTGA CAGTACTTGT 
GATGAAAAGA TAAGAGCCTT TGTTGATAAA GAAATCACTG TTAATTCATA TGTACAAGCG 
TCGTCCTCAC CAGATACAAA AGAAGAGAGC ACAAGTGATG AGCTACATGA CCAGAAAAAT 
GGTCAGGATT ATAAAAGTAG TTGTGAAGAA AAGTTAAGAG CCTTTGTTGA TAAAGAATCA 
ACTGTTAATT CGTATTTACA AGCATCGTCC TTGCCAGATT TCCATAGGAC CCTGAGGACT 
TCACCTGTAA TTGATTCACA ATGTCAGCCT ACCTTGATGG ATCCAGGGGG TGAATTGAAG 
AATGGCATAC TGCAAACAGA AGATAATTTC TGTACATTGA AGGATTGTTC TGCAGATGGA 
AATGCCAACT CTACCATTAG AAAACAATTT TATCCAGAAT CAGGTCAGTC CTCTTTTGTT 
GTTCTCACCA ATAGCTTTCC AAAGGATGCG CCGGATCTCC TTTCCAAGGG CGATGGTGCT 
TCCATCAACA ATGATTGTGC TGTAGACAAT CCTGGACAAA CAAATAATGA TGGAAAAGAA 
GATGTTGAAG TTGATCATAT TACTGAAAAC ATACTGTTAC CTTCTCAAAG GAGTCAAAGA 
ACCAAATTTG GTAGTAAGAC ACAAACCAGG AAGGCTTCTA GAAAATCCAA GAACAAAGCC 
AGTATGACAC ATCGAGGTGG AGGTATGAAT ATGAACTTAG AGGCTGCTAG AAAGAAAAGA 
AGTTGTTTAT GCAAACCGGC TCGTTCTTCT GTCTGGGGAT CGCTTGGGAA TATTGAACAG 
TTTTTTCAGC ACGAAAACGA GCTGGAGGTT AGTGAAGCCA TGTGCCAGAA TGTGGTGAAA 
GCTAGAAGCA AGTTTCAAGG TGGGGAAATG ATTAAGAATG CTACAAGTTC TAGCTCATTA 
AGTTCAGTTC AAAAGGATAA TGTTCCAACT TCAACTACTC GTTTTCGTCT GAAGATCAAG 
TTTGGAAAAG AAAATGCTTT ACGTTGCTCA AACGTCTTGA TTCCGGAGTC TGTTGGTGGC 
TTGGCTTCTG CTTCCTACTT GGAGTCGGAC CCAGGTTCTC GGAAAGTAGC CAGCAATTCT 
GCAGATAAAT TTTCTGAAGC ATTGGCTTTA AGCAATTTAG AATCATTCAG AAATGACCTG 
GACAAGGATT TTGTTGTTCT AAATGGACAA ATTGTAAATA GCCAGTTAGA AAATTCTAAA 
ATAACGGAGA AGTCAGATGA GGATGCAGTG GAACCATGTC ATGCAGGTCC TCCAGAGAAA 
GTGGTTGAAG CATTAGTTAA GCCAATTAAT AATTCAGTTA TAGATCCCGG AACGTCACCT 
GATTCAGAAG TCATTGATTC AATTCCTGAC GTCCAGGTTG GAGAAAGACA TGAAGATGTA 
CATTTTTCTG TGTTAGGTTC TTCTAAGGAG TTAAATTCTC ATATGGACGT TACCTTCAGG 
AATAGAGGGA AGAAGAAAGA CAAGCTTATT TATTCAGGCA ATTGCATCAC TGAAGATGGA 
TCACAAGGTC AACCAGGGAA TAATAGAGCT AAGCATTCGA AGAATCACAG ACGTAAGAGA 
ACCTCCAGTG ATGTGGTTAG CTCTTTAGAA TTGCCTATTT CCACTGAGCT AAGCAAATCT 
GTAAAGAGTA AAGAATTGTC TGCAGAATCA TCGCCCCTTT CTGGAGATAT TGTACTTGGA 
GGCCCTATGG AGTCTTTGAA AGTTAAAAGT CATGCGATAG TCAAGACAGC TGGCAAATCA 
TCTGCTGACC ATGGTTTTAC TGAACTCCAG GGTTCAAAAA ATTTACCGTC TTCTACAAGA 
CCTTGGGGTC GTAAACTACC TAAAAGTCTT GAACCTAGAA AAGTTAGCAA GGGTAAATCC 
AAAGCTTCTG ACTCGACAAG CAGGAAAAAG CCTACTTCTA GGCCCAAGGA GAGACAGAAA 
GAGCCAATTA ACAGAAGTGA AGTAAAGGGA AAAGATGTCT CTCTCAAAGT TACATGTGAA 
GTGGAAGATC ACCCACATCC AGCAGATATA GTTGGAAATC ATAAGTTGGC TGGTGTTGGA 
AAAATTAATA CCGGTGACAA CAGAGTACCA GTCAGTGTGT CTAATTTGGA CGTGATGCCT 
GGTTTTGGTT TGGAAGAACA GCAGCAGTCA CCTCGTAATG CATGGGTGAG TTGTGATGAC 
TGTCACAAGT GGCGGCGCAT TCCAGCTTTG CTTGCAGACC AGATTGATGA AACTAACTGC 
ACCTGGACTT GCAAGGACAG CAGTGATAAA GCCTATGCTG ATTGTGCTAT CCCTCAAGAA 
AAGTCAAATG CAGAGATTAA TGCAGAGTTA GGATTATCAG ACGCCTCTGG TGAAGAAGAT 
GCATATGGAA ACTCCAAAAC TCATAAGGAG TTGGAATATC AACTGCCTTT GGTTTCCCAA 
GAGTCAACTT TTACCCGCAT TTTCACAAAT GAATTTCTGC ATCGTAACCC AAGAACTCAG 
ACTATTGATG AGGTAATGGT TTGCCATTGC AAGCCACCTC GAGAAGGCAA GATGGGATGT 
GGGGATGAAT GTCTAAACCG GATGCTTAAC ATCGAATGTG TGCAAGGAAC TTGCCCATGT 
GGGGACCGTT GTTCCAATCA GCAGTTCCAA AAACGCAATT ATTCCAGACT GAAGTGGTTT 
AAATGCGGGA AAAAAGGTTA TGGACTGAAG GCACTTGAGC GTGTAGCTGA AGGCCAGTTT 
ATTATTGAAT ACGTTGGAGA GGTGCTTGAC GTGCATGCAT ACGAGGCACG CCAGAGAGAG 
TATGCTTTGA AGGGTCATAG ACATTTCTAT TTTATGACCT TGAACGGCAG TGAGGTGATA 
GATGCAAGTG CAAAAGGAAA TTTGGGGCGT TTCATTAATC ATAGTTGTGA TCCTAATTGT 
CGGACAGAAA AGTGGATGGT GAATGGAGAA ATCTGTATTG GACTGTTTGC ATTGAGGAAT 
ATCAAGCAGG ATGAAGAATT GACATTTGAT TACAACTATG TAAGGGTTTT TGGTGCTGCT 
GCCAAAAAGT GTTATTGTGG TTCACTTCAC TGTCAAGGAT ATATAGGCGG TGCGGATCCA 
AATAACGGTG AGCTTATAGT TCAAGGTGAA TCAGATGATG AATTTCCAGA ACCTATGATG 
CTTTCTGAAA ATGGTGAAAT TGACGACAGC GTACTCATGC CCAAATGCAT TGATAGTGTT 
AATACAAAGT CTTCTAGGCA TTTAATAACA GACAGGGATG TATTGGACAA GTGTACAACT 
GCTATATGTG CAGATGGTTC TCCAGAGGAA GACAGTTCTA CAAACCCTGC CTCTGCTGTT 
TCCCTGTTGC ACAGTTCAGT TGAAGTGGAA GACTCAAAAA GCAATTTACC ATCTTCTGAT 
CGAATAGAAG AAATTTCTCA ACAAATAGAG GATACAACAA GCAAACCCAT GCCTGCTGAC 
AGCAAAGAAT TGCCGAATTC TACTGATAGT AATAGAGAAT CCAAATCTGA AATGGTTGAA 
GTCGGGAATG ATTTTTCACA ATCACATCTG CTTGTGAAGA CTCCTCGACT AAATGCTTCT 
GTTAAAAAGG GTAAGGTAAG GGCTAATGCT GCAAATGCCC TTACAGCCGA GGTGGCGGCC 
CCTCGATTGC CAGTATCATC TATCAAAAAC AAAAAAGTAG AAGGTTCTTC CAATGGACGG 
TTTGAAGCAG TTCAGGGGAA ACTTAATGAG TTGCTGGATG GAAATGGAGG AATAAGCAAA 
AGAAAAGATG CCACCAAAGG CTACTTGAAA CTTTTGCTTC TCACCGTGGC ATCAGGCGAT 
AGAAGCAATG GTGAAGCAAT TCAGAGTAAC CGAGATCTTT CCATGATCCT TGATGCTCTT 
CTGAAAACAA AATCGAGAGC CGTGCTAAAT GATATTATTA GTAAAAATGG TTTACAGATG 
TTACATAAGA TCATGAAGCA GTATAGGCAA GACTTCAAAA AAATTCCAAT ACTACGAAAG 
CTTCTTAAGG TCTTAGAGTA CCTGGCAGCA GGCAAGATTT TGACTCCTGA ACATATCAAT 
GGTGGTCCTC CTTGTCATGG AATGGAGAGG TTTAGGGATT CAATGCTGTC TCTGACAGAG 
CATGATGATA AACAGGTCCA TCAAATTGCT CGAAGCTTCC GAGACAGATG GATTCCAAGA 
CATGGCAGAA AACATGGCTA TATGGACAGG GATGATAACA GGATGGAATC TCACAGAGGT 
TTCAACTCCA ACAGATTTTC AGTATCACAC AGTCATAGGC ACGAGCAGGG ATTAAGACCT 
AAGGAAGCAA CTGATTGTGG TCAGCAGCCA ATGCTTGTGG CAACAGTAGA CGCGCGTGCC 
CAGGAGGGCT GCTCTACACC ATCTTTGGAT GGAGTTGAGA TCAACGGGGC AAAAAAACGT 
AAGCGCAAAA GCCGATGGGA TCAACCAGCA GAAACAAATT CATATTCTGA TGCTATCATT 
AGTTCTATTA ATGAAAGCCA GAACGTTCAT GAAGAAGTTC CACCCGGATT CTCATGTCCA 
ATACGTTCAT TAAACAGTGC ACTAAACTCT GGTACTCCTG CTTTGCAAAA TGCAAGCCAT 
TCTGGATGCC CCCCAAGTTT AGTTATTGGT CAGCCAAAAG AGAAATTCAA TTCTCGTTTG 
CCTGTCTCAT ATGGATTGCC ATGGTCTGTT GCGCAACAAT ATGGAACTCC TCATGCTGAA 
ATCACAGGGT GTTGGATCAC TGCTCCTGGC ATGCCTTTCA ACCCGTTTCC TCCATTACCC 
CCATATCCAC GGGATAACAA AGACTGTCAA CCTTCCAGTA TGGAAATTGA TCAGCCTGCT 
GAAGTTAAGC AATCGGACGC CACTGGTCCG GTTAATTGCT GCTCAGATGA TATGATTCCC 
AGCACAACTG GTGCTAACTC TGAAGACACG AACCTTCAAT GTGAGGATGC TAAACATGAC 
GCTAAGCGAT TGAAGGGTGA TGATTCTGAT GATTTGGGAA AGAAGTACTT TAGACAGCAG 
AAATGGAATA ATTCAAAAAT ACATCGAACA TGGTTCAAAA GGGATGCATG GAAATGTAAT 
GGGAACAGCT CTAGTGGTGA TATTTGCAGT ATAGATGTAG GAGATGTGTC AAAAGAATCA 
AAAGTTACTA GTTATTCAGA GGAGGATGAA ATCTGTAGAG AGGAGAAAGG TGGAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101503412 ( XM_004502484.2 ) cDNA ORF clone, Cicer arietinum(chickpea) -> XP_004502541.1 Cicer arietinum histone-lysine N-methyltransferase ASHH2 (LOC101503412), transcript variant X3, mRNA.

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CloneID	OCi113902
Clone ID Related Accession (Same CDS sequence)	XM_004502484.2 , XM_004502484.3
Accession Version	XM_004502484.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-06-07
Organism	Cicer arietinum(chickpea)
Product	histone-lysine N-methyltransferase ASHH2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004515485.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 8, 2015 this sequence version replaced XM_004502484.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cicer arietinum Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTCGT GTGGTGAACC GGTAATCGTT GGTGATCCGG CACAATTGGA GCAGCCTTTG 
TGCTCGGGTT TTCCGGCACA GTTAGTTTCT GTGCAGGAGT CATGTTCAGA AGAACCTTGC 
GATGTTTTAG ACGTATCTGG TATGGCGGAT GGTTTTTTGG GAGAGGGTGA AGTGGGTTGT 
TCTGCAAGCT ATCTTGATGT TATGGAGGGG TACGGAGATG CTTTGACAAA TGAATGTGAA 
AATGATGATA TGTCACAATT GGAGAAAACT GCTGAAGATG GTTGTTGGAA TAGTTTGGGG 
ATTTGTTCTG AGGACATTGA AGTTTCCGGT CTTAAAAGTG GTGGATTGTG CTTAGAAGGA 
AATTTTAAAG ATGAAGGAAA CCTTGACCTG CCGTTGGAGT CCATAACCTC AAATGATTTG 
CAGAAGCATT GGGCTGAACA GGGTGAGCAG AAGAATGGCA AGAACATTAT GGTATTCTCG 
GTAGGAGATG ATTTGACAGT CGTGGAAGGC AAAAACGATG GTGCTGATTT ACTAGCTGGT 
GCTGTCAACT GTGTGCTTGA TGTTGAGCAT TCTGAAGTGC CTTTGGAATC AGATAGCTGG 
CCAAAGGCTG TGGAAAATTG TGATGCTTTA GATGGGATAG AAGCGAATTC TTGCAGGCAG 
ATATCTTCTT CACTAAATTT TGAAGTGCCG TCAGGTGTCT TGTTTATAGA TGCAAAAGTA 
GAGAGCACAA GTGATAAGCT ACACGATCTA AAAAATGGCG AGGATTGTGA CAGTACTTGT 
GATGAAAAGA TAAGAGCCTT TGTTGATAAA GAAATCACTG TTAATTCATA TGTACAAGCG 
TCGTCCTCAC CAGATACAAA AGAAGAGAGC ACAAGTGATG AGCTACATGA CCAGAAAAAT 
GGTCAGGATT ATAAAAGTAG TTGTGAAGAA AAGTTAAGAG CCTTTGTTGA TAAAGAATCA 
ACTGTTAATT CGTATTTACA AGCATCGTCC TTGCCAGATT TCCATAGGAC CCTGAGGACT 
TCACCTGTAA TTGATTCACA ATGTCAGCCT ACCTTGATGG ATCCAGGGGG TGAATTGAAG 
AATGGCATAC TGCAAACAGA AGATAATTTC TGTACATTGA AGGATTGTTC TGCAGATGGA 
AATGCCAACT CTACCATTAG AAAACAATTT TATCCAGAAT CAGGTCAGTC CTCTTTTGTT 
GTTCTCACCA ATAGCTTTCC AAAGGATGCG CCGGATCTCC TTTCCAAGGG CGATGGTGCT 
TCCATCAACA ATGATTGTGC TGTAGACAAT CCTGGACAAA CAAATAATGA TGGAAAAGAA 
GATGTTGAAG TTGATCATAT TACTGAAAAC ATACTGTTAC CTTCTCAAAG GAGTCAAAGA 
ACCAAATTTG GTAGTAAGAC ACAAACCAGG AAGGCTTCTA GAAAATCCAA GAACAAAGCC 
AGTATGACAC ATCGAGGTGG AGGTATGAAT ATGAACTTAG AGGCTGCTAG AAAGAAAAGA 
AGTTGTTTAT GCAAACCGGC TCGTTCTTCT GTCTGGGGAT CGCTTGGGAA TATTGAACAG 
TTTTTTCAGC ACGAAAACGA GCTGGAGGTT AGTGAAGCCA TGTGCCAGAA TGTGGTGAAA 
GCTAGAAGCA AGTTTCAAGG TGGGGAAATG ATTAAGAATG CTACAAGTTC TAGCTCATTA 
AGTTCAGTTC AAAAGGATAA TGTTCCAACT TCAACTACTC GTTTTCGTCT GAAGATCAAG 
TTTGGAAAAG AAAATGCTTT ACGTTGCTCA AACGTCTTGA TTCCGGAGTC TGTTGGTGGC 
TTGGCTTCTG CTTCCTACTT GGAGTCGGAC CCAGGTTCTC GGAAAGTAGC CAGCAATTCT 
GCAGATAAAT TTTCTGAAGC ATTGGCTTTA AGCAATTTAG AATCATTCAG AAATGACCTG 
GACAAGGATT TTGTTGTTCT AAATGGACAA ATTGTAAATA GCCAGTTAGA AAATTCTAAA 
ATAACGGAGA AGTCAGATGA GGATGCAGTG GAACCATGTC ATGCAGGTCC TCCAGAGAAA 
GTGGTTGAAG CATTAGTTAA GCCAATTAAT AATTCAGTTA TAGATCCCGG AACGTCACCT 
GATTCAGAAG TCATTGATTC AATTCCTGAC GTCCAGGTTG GAGAAAGACA TGAAGATGTA 
CATTTTTCTG TGTTAGGTTC TTCTAAGGAG TTAAATTCTC ATATGGACGT TACCTTCAGG 
AATAGAGGGA AGAAGAAAGA CAAGCTTATT TATTCAGGCA ATTGCATCAC TGAAGATGGA 
TCACAAGGTC AACCAGGGAA TAATAGAGCT AAGCATTCGA AGAATCACAG ACGTAAGAGA 
ACCTCCAGTG ATGTGGTTAG CTCTTTAGAA TTGCCTATTT CCACTGAGCT AAGCAAATCT 
GTAAAGAGTA AAGAATTGTC TGCAGAATCA TCGCCCCTTT CTGGAGATAT TGTACTTGGA 
GGCCCTATGG AGTCTTTGAA AGTTAAAAGT CATGCGATAG TCAAGACAGC TGGCAAATCA 
TCTGCTGACC ATGGTTTTAC TGAACTCCAG GGTTCAAAAA ATTTACCGTC TTCTACAAGA 
CCTTGGGGTC GTAAACTACC TAAAAGTCTT GAACCTAGAA AAGTTAGCAA GGGTAAATCC 
AAAGCTTCTG ACTCGACAAG CAGGAAAAAG CCTACTTCTA GGCCCAAGGA GAGACAGAAA 
GAGCCAATTA ACAGAAGTGA AGTAAAGGGA AAAGATGTCT CTCTCAAAGT TACATGTGAA 
GTGGAAGATC ACCCACATCC AGATATAGTT GGAAATCATA AGTTGGCTGG TGTTGGAAAA 
ATTAATACCG GTGACAACAG AGTACCAGTC AGTGTGTCTA ATTTGGACGT GATGCCTGGT 
TTTGGTTTGG AAGAACAGCA GCAGTCACCT CGTAATGCAT GGGTGAGTTG TGATGACTGT 
CACAAGTGGC GGCGCATTCC AGCTTTGCTT GCAGACCAGA TTGATGAAAC TAACTGCACC 
TGGACTTGCA AGGACAGCAG TGATAAAGCC TATGCTGATT GTGCTATCCC TCAAGAAAAG 
TCAAATGCAG AGATTAATGC AGAGTTAGGA TTATCAGACG CCTCTGGTGA AGAAGATGCA 
TATGGAAACT CCAAAACTCA TAAGGAGTTG GAATATCAAC TGCCTTTGGT TTCCCAAGAG 
TCAACTTTTA CCCGCATTTT CACAAATGAA TTTCTGCATC GTAACCCAAG AACTCAGACT 
ATTGATGAGG TAATGGTTTG CCATTGCAAG CCACCTCGAG AAGGCAAGAT GGGATGTGGG 
GATGAATGTC TAAACCGGAT GCTTAACATC GAATGTGTGC AAGGAACTTG CCCATGTGGG 
GACCGTTGTT CCAATCAGCA GTTCCAAAAA CGCAATTATT CCAGACTGAA GTGGTTTAAA 
TGCGGGAAAA AAGGTTATGG ACTGAAGGCA CTTGAGCGTG TAGCTGAAGG CCAGTTTATT 
ATTGAATACG TTGGAGAGGT GCTTGACGTG CATGCATACG AGGCACGCCA GAGAGAGTAT 
GCTTTGAAGG GTCATAGACA TTTCTATTTT ATGACCTTGA ACGGCAGTGA GGTGATAGAT 
GCAAGTGCAA AAGGAAATTT GGGGCGTTTC ATTAATCATA GTTGTGATCC TAATTGTCGG 
ACAGAAAAGT GGATGGTGAA TGGAGAAATC TGTATTGGAC TGTTTGCATT GAGGAATATC 
AAGCAGGATG AAGAATTGAC ATTTGATTAC AACTATGTAA GGGTTTTTGG TGCTGCTGCC 
AAAAAGTGTT ATTGTGGTTC ACTTCACTGT CAAGGATATA TAGGCGGTGC GGATCCAAAT 
AACGGTGAGC TTATAGTTCA AGGTGAATCA GATGATGAAT TTCCAGAACC TATGATGCTT 
TCTGAAAATG GTGAAATTGA CGACAGCGTA CTCATGCCCA AATGCATTGA TAGTGTTAAT 
ACAAAGTCTT CTAGGCATTT AATAACAGAC AGGGATGTAT TGGACAAGTG TACAACTGCT 
ATATGTGCAG ATGGTTCTCC AGAGGAAGAC AGTTCTACAA ACCCTGCCTC TGCTGTTTCC 
CTGTTGCACA GTTCAGTTGA AGTGGAAGAC TCAAAAAGCA ATTTACCATC TTCTGATCGA 
ATAGAAGAAA TTTCTCAACA AATAGAGGAT ACAACAAGCA AACCCATGCC TGCTGACAGC 
AAAGAATTGC CGAATTCTAC TGATAGTAAT AGAGAATCCA AATCTGAAAT GGTTGAAGTC 
GGGAATGATT TTTCACAATC ACATCTGCTT GTGAAGACTC CTCGACTAAA TGCTTCTGTT 
AAAAAGGGTA AGGTAAGGGC TAATGCTGCA AATGCCCTTA CAGCCGAGGT GGCGGCCCCT 
CGATTGCCAG TATCATCTAT CAAAAACAAA AAAGTAGAAG GTTCTTCCAA TGGACGGTTT 
GAAGCAGTTC AGGGGAAACT TAATGAGTTG CTGGATGGAA ATGGAGGAAT AAGCAAAAGA 
AAAGATGCCA CCAAAGGCTA CTTGAAACTT TTGCTTCTCA CCGTGGCATC AGGCGATAGA 
AGCAATGGTG AAGCAATTCA GAGTAACCGA GATCTTTCCA TGATCCTTGA TGCTCTTCTG 
AAAACAAAAT CGAGAGCCGT GCTAAATGAT ATTATTAGTA AAAATGGTTT ACAGATGTTA 
CATAAGATCA TGAAGCAGTA TAGGCAAGAC TTCAAAAAAA TTCCAATACT ACGAAAGCTT 
CTTAAGGTCT TAGAGTACCT GGCAGCAGGC AAGATTTTGA CTCCTGAACA TATCAATGGT 
GGTCCTCCTT GTCATGGAAT GGAGAGGTTT AGGGATTCAA TGCTGTCTCT GACAGAGCAT 
GATGATAAAC AGGTCCATCA AATTGCTCGA AGCTTCCGAG ACAGATGGAT TCCAAGACAT 
GGCAGAAAAC ATGGCTATAT GGACAGGGAT GATAACAGGA TGGAATCTCA CAGAGGTTTC 
AACTCCAACA GATTTTCAGT ATCACACAGT CATAGGCACG AGCAGGGATT AAGACCTAAG 
GAAGCAACTG ATTGTGGTCA GCAGCCAATG CTTGTGGCAA CAGTAGACGC GCGTGCCCAG 
GAGGGCTGCT CTACACCATC TTTGGATGGA GTTGAGATCA ACGGGGCAAA AAAACGTAAG 
CGCAAAAGCC GATGGGATCA ACCAGCAGAA ACAAATTCAT ATTCTGATGC TATCATTAGT 
TCTATTAATG AAAGCCAGAA CGTTCATGAA GAAGTTCCAC CCGGATTCTC ATGTCCAATA 
CGTTCATTAA ACAGTGCACT AAACTCTGGT ACTCCTGCTT TGCAAAATGC AAGCCATTCT 
GGATGCCCCC CAAGTTTAGT TATTGGTCAG CCAAAAGAGA AATTCAATTC TCGTTTGCCT 
GTCTCATATG GATTGCCATG GTCTGTTGCG CAACAATATG GAACTCCTCA TGCTGAAATC 
ACAGGGTGTT GGATCACTGC TCCTGGCATG CCTTTCAACC CGTTTCCTCC ATTACCCCCA 
TATCCACGGG ATAACAAAGA CTGTCAACCT TCCAGTATGG AAATTGATCA GCCTGCTGAA 
GTTAAGCAAT CGGACGCCAC TGGTCCGGTT AATTGCTGCT CAGATGATAT GATTCCCAGC 
ACAACTGGTG CTAACTCTGA AGACACGAAC CTTCAATGTG AGGATGCTAA ACATGACGCT 
AAGCGATTGA AGGGTGATGA TTCTGATGAT TTGGGAAAGA AGTACTTTAG ACAGCAGAAA 
TGGAATAATT CAAAAATACA TCGAACATGG TTCAAAAGGG ATGCATGGAA ATGTAATGGG 
AACAGCTCTA GTGGTGATAT TTGCAGTATA GATGTAGGAG ATGTGTCAAA AGAATCAAAA 
GTTACTAGTT ATTCAGAGGA GGATGAAATC TGTAGAGAGG AGAAAGGTGG AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004502541.1
CDS	364..6300
Translation	MGSCGEPVIVGDPAQLEQPLCSGFPAQLVSVQESCSEEPCDVLDVSGMADGFLGEGEVGCSASYLDVMEGYGDALTNECENDDMSQLEKTAEDGCWNSLGICSEDIEVSGLKSGGLCLEGNFKDEGNLDLPLESITSNDLQKHWAEQGEQKNGKNIMVFSVGDDLTVVEGKNDGADLLAGAVNCVLDVEHSEVPLESDSWPKAVENCDALDGIEANSCRQISSSLNFEVPSGVLFIDAKVESTSDKLHDLKNGEDCDSTCDEKIRAFVDKEITVNSYVQASSSPDTKEESTSDELHDQKNGQDYKSSCEEKLRAFVDKESTVNSYLQASSLPDFHRTLRTSPVIDSQCQPTLMDPGGELKNGILQTEDNFCTLKDCSADGNANSTIRKQFYPESGQSSFVVLTNSFPKDAPDLLSKGDGASINNDCAVDNPGQTNNDGKEDVEVDHITENILLPSQRSQRTKFGSKTQTRKASRKSKNKASMTHRGGGMNMNLEAARKKRSCLCKPARSSVWGSLGNIEQFFQHENELEVSEAMCQNVVKARSKFQGGEMIKNATSSSSLSSVQKDNVPTSTTRFRLKIKFGKENALRCSNVLIPESVGGLASASYLESDPGSRKVASNSADKFSEALALSNLESFRNDLDKDFVVLNGQIVNSQLENSKITEKSDEDAVEPCHAGPPEKVVEALVKPINNSVIDPGTSPDSEVIDSIPDVQVGERHEDVHFSVLGSSKELNSHMDVTFRNRGKKKDKLIYSGNCITEDGSQGQPGNNRAKHSKNHRRKRTSSDVVSSLELPISTELSKSVKSKELSAESSPLSGDIVLGGPMESLKVKSHAIVKTAGKSSADHGFTELQGSKNLPSSTRPWGRKLPKSLEPRKVSKGKSKASDSTSRKKPTSRPKERQKEPINRSEVKGKDVSLKVTCEVEDHPHPDIVGNHKLAGVGKINTGDNRVPVSVSNLDVMPGFGLEEQQQSPRNAWVSCDDCHKWRRIPALLADQIDETNCTWTCKDSSDKAYADCAIPQEKSNAEINAELGLSDASGEEDAYGNSKTHKELEYQLPLVSQESTFTRIFTNEFLHRNPRTQTIDEVMVCHCKPPREGKMGCGDECLNRMLNIECVQGTCPCGDRCSNQQFQKRNYSRLKWFKCGKKGYGLKALERVAEGQFIIEYVGEVLDVHAYEARQREYALKGHRHFYFMTLNGSEVIDASAKGNLGRFINHSCDPNCRTEKWMVNGEICIGLFALRNIKQDEELTFDYNYVRVFGAAAKKCYCGSLHCQGYIGGADPNNGELIVQGESDDEFPEPMMLSENGEIDDSVLMPKCIDSVNTKSSRHLITDRDVLDKCTTAICADGSPEEDSSTNPASAVSLLHSSVEVEDSKSNLPSSDRIEEISQQIEDTTSKPMPADSKELPNSTDSNRESKSEMVEVGNDFSQSHLLVKTPRLNASVKKGKVRANAANALTAEVAAPRLPVSSIKNKKVEGSSNGRFEAVQGKLNELLDGNGGISKRKDATKGYLKLLLLTVASGDRSNGEAIQSNRDLSMILDALLKTKSRAVLNDIISKNGLQMLHKIMKQYRQDFKKIPILRKLLKVLEYLAAGKILTPEHINGGPPCHGMERFRDSMLSLTEHDDKQVHQIARSFRDRWIPRHGRKHGYMDRDDNRMESHRGFNSNRFSVSHSHRHEQGLRPKEATDCGQQPMLVATVDARAQEGCSTPSLDGVEINGAKKRKRKSRWDQPAETNSYSDAIISSINESQNVHEEVPPGFSCPIRSLNSALNSGTPALQNASHSGCPPSLVIGQPKEKFNSRLPVSYGLPWSVAQQYGTPHAEITGCWITAPGMPFNPFPPLPPYPRDNKDCQPSSMEIDQPAEVKQSDATGPVNCCSDDMIPSTTGANSEDTNLQCEDAKHDAKRLKGDDSDDLGKKYFRQQKWNNSKIHRTWFKRDAWKCNGNSSSGDICSIDVGDVSKESKVTSYSEEDEICREEKGGK

Target ORF information:

RefSeq Version	XM_004502484.2
Organism	Cicer arietinum(chickpea)
Definition	Cicer arietinum histone-lysine N-methyltransferase ASHH2 (LOC101503412), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004502484.2

ORF Insert Sequence:

ATGGGCTCGT GTGGTGAACC GGTAATCGTT GGTGATCCGG CACAATTGGA GCAGCCTTTG 
TGCTCGGGTT TTCCGGCACA GTTAGTTTCT GTGCAGGAGT CATGTTCAGA AGAACCTTGC 
GATGTTTTAG ACGTATCTGG TATGGCGGAT GGTTTTTTGG GAGAGGGTGA AGTGGGTTGT 
TCTGCAAGCT ATCTTGATGT TATGGAGGGG TACGGAGATG CTTTGACAAA TGAATGTGAA 
AATGATGATA TGTCACAATT GGAGAAAACT GCTGAAGATG GTTGTTGGAA TAGTTTGGGG 
ATTTGTTCTG AGGACATTGA AGTTTCCGGT CTTAAAAGTG GTGGATTGTG CTTAGAAGGA 
AATTTTAAAG ATGAAGGAAA CCTTGACCTG CCGTTGGAGT CCATAACCTC AAATGATTTG 
CAGAAGCATT GGGCTGAACA GGGTGAGCAG AAGAATGGCA AGAACATTAT GGTATTCTCG 
GTAGGAGATG ATTTGACAGT CGTGGAAGGC AAAAACGATG GTGCTGATTT ACTAGCTGGT 
GCTGTCAACT GTGTGCTTGA TGTTGAGCAT TCTGAAGTGC CTTTGGAATC AGATAGCTGG 
CCAAAGGCTG TGGAAAATTG TGATGCTTTA GATGGGATAG AAGCGAATTC TTGCAGGCAG 
ATATCTTCTT CACTAAATTT TGAAGTGCCG TCAGGTGTCT TGTTTATAGA TGCAAAAGTA 
GAGAGCACAA GTGATAAGCT ACACGATCTA AAAAATGGCG AGGATTGTGA CAGTACTTGT 
GATGAAAAGA TAAGAGCCTT TGTTGATAAA GAAATCACTG TTAATTCATA TGTACAAGCG 
TCGTCCTCAC CAGATACAAA AGAAGAGAGC ACAAGTGATG AGCTACATGA CCAGAAAAAT 
GGTCAGGATT ATAAAAGTAG TTGTGAAGAA AAGTTAAGAG CCTTTGTTGA TAAAGAATCA 
ACTGTTAATT CGTATTTACA AGCATCGTCC TTGCCAGATT TCCATAGGAC CCTGAGGACT 
TCACCTGTAA TTGATTCACA ATGTCAGCCT ACCTTGATGG ATCCAGGGGG TGAATTGAAG 
AATGGCATAC TGCAAACAGA AGATAATTTC TGTACATTGA AGGATTGTTC TGCAGATGGA 
AATGCCAACT CTACCATTAG AAAACAATTT TATCCAGAAT CAGGTCAGTC CTCTTTTGTT 
GTTCTCACCA ATAGCTTTCC AAAGGATGCG CCGGATCTCC TTTCCAAGGG CGATGGTGCT 
TCCATCAACA ATGATTGTGC TGTAGACAAT CCTGGACAAA CAAATAATGA TGGAAAAGAA 
GATGTTGAAG TTGATCATAT TACTGAAAAC ATACTGTTAC CTTCTCAAAG GAGTCAAAGA 
ACCAAATTTG GTAGTAAGAC ACAAACCAGG AAGGCTTCTA GAAAATCCAA GAACAAAGCC 
AGTATGACAC ATCGAGGTGG AGGTATGAAT ATGAACTTAG AGGCTGCTAG AAAGAAAAGA 
AGTTGTTTAT GCAAACCGGC TCGTTCTTCT GTCTGGGGAT CGCTTGGGAA TATTGAACAG 
TTTTTTCAGC ACGAAAACGA GCTGGAGGTT AGTGAAGCCA TGTGCCAGAA TGTGGTGAAA 
GCTAGAAGCA AGTTTCAAGG TGGGGAAATG ATTAAGAATG CTACAAGTTC TAGCTCATTA 
AGTTCAGTTC AAAAGGATAA TGTTCCAACT TCAACTACTC GTTTTCGTCT GAAGATCAAG 
TTTGGAAAAG AAAATGCTTT ACGTTGCTCA AACGTCTTGA TTCCGGAGTC TGTTGGTGGC 
TTGGCTTCTG CTTCCTACTT GGAGTCGGAC CCAGGTTCTC GGAAAGTAGC CAGCAATTCT 
GCAGATAAAT TTTCTGAAGC ATTGGCTTTA AGCAATTTAG AATCATTCAG AAATGACCTG 
GACAAGGATT TTGTTGTTCT AAATGGACAA ATTGTAAATA GCCAGTTAGA AAATTCTAAA 
ATAACGGAGA AGTCAGATGA GGATGCAGTG GAACCATGTC ATGCAGGTCC TCCAGAGAAA 
GTGGTTGAAG CATTAGTTAA GCCAATTAAT AATTCAGTTA TAGATCCCGG AACGTCACCT 
GATTCAGAAG TCATTGATTC AATTCCTGAC GTCCAGGTTG GAGAAAGACA TGAAGATGTA 
CATTTTTCTG TGTTAGGTTC TTCTAAGGAG TTAAATTCTC ATATGGACGT TACCTTCAGG 
AATAGAGGGA AGAAGAAAGA CAAGCTTATT TATTCAGGCA ATTGCATCAC TGAAGATGGA 
TCACAAGGTC AACCAGGGAA TAATAGAGCT AAGCATTCGA AGAATCACAG ACGTAAGAGA 
ACCTCCAGTG ATGTGGTTAG CTCTTTAGAA TTGCCTATTT CCACTGAGCT AAGCAAATCT 
GTAAAGAGTA AAGAATTGTC TGCAGAATCA TCGCCCCTTT CTGGAGATAT TGTACTTGGA 
GGCCCTATGG AGTCTTTGAA AGTTAAAAGT CATGCGATAG TCAAGACAGC TGGCAAATCA 
TCTGCTGACC ATGGTTTTAC TGAACTCCAG GGTTCAAAAA ATTTACCGTC TTCTACAAGA 
CCTTGGGGTC GTAAACTACC TAAAAGTCTT GAACCTAGAA AAGTTAGCAA GGGTAAATCC 
AAAGCTTCTG ACTCGACAAG CAGGAAAAAG CCTACTTCTA GGCCCAAGGA GAGACAGAAA 
GAGCCAATTA ACAGAAGTGA AGTAAAGGGA AAAGATGTCT CTCTCAAAGT TACATGTGAA 
GTGGAAGATC ACCCACATCC AGATATAGTT GGAAATCATA AGTTGGCTGG TGTTGGAAAA 
ATTAATACCG GTGACAACAG AGTACCAGTC AGTGTGTCTA ATTTGGACGT GATGCCTGGT 
TTTGGTTTGG AAGAACAGCA GCAGTCACCT CGTAATGCAT GGGTGAGTTG TGATGACTGT 
CACAAGTGGC GGCGCATTCC AGCTTTGCTT GCAGACCAGA TTGATGAAAC TAACTGCACC 
TGGACTTGCA AGGACAGCAG TGATAAAGCC TATGCTGATT GTGCTATCCC TCAAGAAAAG 
TCAAATGCAG AGATTAATGC AGAGTTAGGA TTATCAGACG CCTCTGGTGA AGAAGATGCA 
TATGGAAACT CCAAAACTCA TAAGGAGTTG GAATATCAAC TGCCTTTGGT TTCCCAAGAG 
TCAACTTTTA CCCGCATTTT CACAAATGAA TTTCTGCATC GTAACCCAAG AACTCAGACT 
ATTGATGAGG TAATGGTTTG CCATTGCAAG CCACCTCGAG AAGGCAAGAT GGGATGTGGG 
GATGAATGTC TAAACCGGAT GCTTAACATC GAATGTGTGC AAGGAACTTG CCCATGTGGG 
GACCGTTGTT CCAATCAGCA GTTCCAAAAA CGCAATTATT CCAGACTGAA GTGGTTTAAA 
TGCGGGAAAA AAGGTTATGG ACTGAAGGCA CTTGAGCGTG TAGCTGAAGG CCAGTTTATT 
ATTGAATACG TTGGAGAGGT GCTTGACGTG CATGCATACG AGGCACGCCA GAGAGAGTAT 
GCTTTGAAGG GTCATAGACA TTTCTATTTT ATGACCTTGA ACGGCAGTGA GGTGATAGAT 
GCAAGTGCAA AAGGAAATTT GGGGCGTTTC ATTAATCATA GTTGTGATCC TAATTGTCGG 
ACAGAAAAGT GGATGGTGAA TGGAGAAATC TGTATTGGAC TGTTTGCATT GAGGAATATC 
AAGCAGGATG AAGAATTGAC ATTTGATTAC AACTATGTAA GGGTTTTTGG TGCTGCTGCC 
AAAAAGTGTT ATTGTGGTTC ACTTCACTGT CAAGGATATA TAGGCGGTGC GGATCCAAAT 
AACGGTGAGC TTATAGTTCA AGGTGAATCA GATGATGAAT TTCCAGAACC TATGATGCTT 
TCTGAAAATG GTGAAATTGA CGACAGCGTA CTCATGCCCA AATGCATTGA TAGTGTTAAT 
ACAAAGTCTT CTAGGCATTT AATAACAGAC AGGGATGTAT TGGACAAGTG TACAACTGCT 
ATATGTGCAG ATGGTTCTCC AGAGGAAGAC AGTTCTACAA ACCCTGCCTC TGCTGTTTCC 
CTGTTGCACA GTTCAGTTGA AGTGGAAGAC TCAAAAAGCA ATTTACCATC TTCTGATCGA 
ATAGAAGAAA TTTCTCAACA AATAGAGGAT ACAACAAGCA AACCCATGCC TGCTGACAGC 
AAAGAATTGC CGAATTCTAC TGATAGTAAT AGAGAATCCA AATCTGAAAT GGTTGAAGTC 
GGGAATGATT TTTCACAATC ACATCTGCTT GTGAAGACTC CTCGACTAAA TGCTTCTGTT 
AAAAAGGGTA AGGTAAGGGC TAATGCTGCA AATGCCCTTA CAGCCGAGGT GGCGGCCCCT 
CGATTGCCAG TATCATCTAT CAAAAACAAA AAAGTAGAAG GTTCTTCCAA TGGACGGTTT 
GAAGCAGTTC AGGGGAAACT TAATGAGTTG CTGGATGGAA ATGGAGGAAT AAGCAAAAGA 
AAAGATGCCA CCAAAGGCTA CTTGAAACTT TTGCTTCTCA CCGTGGCATC AGGCGATAGA 
AGCAATGGTG AAGCAATTCA GAGTAACCGA GATCTTTCCA TGATCCTTGA TGCTCTTCTG 
AAAACAAAAT CGAGAGCCGT GCTAAATGAT ATTATTAGTA AAAATGGTTT ACAGATGTTA 
CATAAGATCA TGAAGCAGTA TAGGCAAGAC TTCAAAAAAA TTCCAATACT ACGAAAGCTT 
CTTAAGGTCT TAGAGTACCT GGCAGCAGGC AAGATTTTGA CTCCTGAACA TATCAATGGT 
GGTCCTCCTT GTCATGGAAT GGAGAGGTTT AGGGATTCAA TGCTGTCTCT GACAGAGCAT 
GATGATAAAC AGGTCCATCA AATTGCTCGA AGCTTCCGAG ACAGATGGAT TCCAAGACAT 
GGCAGAAAAC ATGGCTATAT GGACAGGGAT GATAACAGGA TGGAATCTCA CAGAGGTTTC 
AACTCCAACA GATTTTCAGT ATCACACAGT CATAGGCACG AGCAGGGATT AAGACCTAAG 
GAAGCAACTG ATTGTGGTCA GCAGCCAATG CTTGTGGCAA CAGTAGACGC GCGTGCCCAG 
GAGGGCTGCT CTACACCATC TTTGGATGGA GTTGAGATCA ACGGGGCAAA AAAACGTAAG 
CGCAAAAGCC GATGGGATCA ACCAGCAGAA ACAAATTCAT ATTCTGATGC TATCATTAGT 
TCTATTAATG AAAGCCAGAA CGTTCATGAA GAAGTTCCAC CCGGATTCTC ATGTCCAATA 
CGTTCATTAA ACAGTGCACT AAACTCTGGT ACTCCTGCTT TGCAAAATGC AAGCCATTCT 
GGATGCCCCC CAAGTTTAGT TATTGGTCAG CCAAAAGAGA AATTCAATTC TCGTTTGCCT 
GTCTCATATG GATTGCCATG GTCTGTTGCG CAACAATATG GAACTCCTCA TGCTGAAATC 
ACAGGGTGTT GGATCACTGC TCCTGGCATG CCTTTCAACC CGTTTCCTCC ATTACCCCCA 
TATCCACGGG ATAACAAAGA CTGTCAACCT TCCAGTATGG AAATTGATCA GCCTGCTGAA 
GTTAAGCAAT CGGACGCCAC TGGTCCGGTT AATTGCTGCT CAGATGATAT GATTCCCAGC 
ACAACTGGTG CTAACTCTGA AGACACGAAC CTTCAATGTG AGGATGCTAA ACATGACGCT 
AAGCGATTGA AGGGTGATGA TTCTGATGAT TTGGGAAAGA AGTACTTTAG ACAGCAGAAA 
TGGAATAATT CAAAAATACA TCGAACATGG TTCAAAAGGG ATGCATGGAA ATGTAATGGG 
AACAGCTCTA GTGGTGATAT TTGCAGTATA GATGTAGGAG ATGTGTCAAA AGAATCAAAA 
GTTACTAGTT ATTCAGAGGA GGATGAAATC TGTAGAGAGG AGAAAGGTGG AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101503412 ( XM_004502484.3 ) cDNA ORF clone, Cicer arietinum(chickpea) -> XP_004502541.1 Cicer arietinum histone-lysine N-methyltransferase ASHH2 (LOC101503412), transcript variant X3, mRNA.

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CloneID	OCi113902
Clone ID Related Accession (Same CDS sequence)	XM_004502484.2 , XM_004502484.3
Accession Version	XM_004502484.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-13
Organism	Cicer arietinum(chickpea)
Product	histone-lysine N-methyltransferase ASHH2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_021164.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 14, 2018 this sequence version replaced XM_004502484.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cicer arietinum Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTCGT GTGGTGAACC GGTAATCGTT GGTGATCCGG CACAATTGGA GCAGCCTTTG 
TGCTCGGGTT TTCCGGCACA GTTAGTTTCT GTGCAGGAGT CATGTTCAGA AGAACCTTGC 
GATGTTTTAG ACGTATCTGG TATGGCGGAT GGTTTTTTGG GAGAGGGTGA AGTGGGTTGT 
TCTGCAAGCT ATCTTGATGT TATGGAGGGG TACGGAGATG CTTTGACAAA TGAATGTGAA 
AATGATGATA TGTCACAATT GGAGAAAACT GCTGAAGATG GTTGTTGGAA TAGTTTGGGG 
ATTTGTTCTG AGGACATTGA AGTTTCCGGT CTTAAAAGTG GTGGATTGTG CTTAGAAGGA 
AATTTTAAAG ATGAAGGAAA CCTTGACCTG CCGTTGGAGT CCATAACCTC AAATGATTTG 
CAGAAGCATT GGGCTGAACA GGGTGAGCAG AAGAATGGCA AGAACATTAT GGTATTCTCG 
GTAGGAGATG ATTTGACAGT CGTGGAAGGC AAAAACGATG GTGCTGATTT ACTAGCTGGT 
GCTGTCAACT GTGTGCTTGA TGTTGAGCAT TCTGAAGTGC CTTTGGAATC AGATAGCTGG 
CCAAAGGCTG TGGAAAATTG TGATGCTTTA GATGGGATAG AAGCGAATTC TTGCAGGCAG 
ATATCTTCTT CACTAAATTT TGAAGTGCCG TCAGGTGTCT TGTTTATAGA TGCAAAAGTA 
GAGAGCACAA GTGATAAGCT ACACGATCTA AAAAATGGCG AGGATTGTGA CAGTACTTGT 
GATGAAAAGA TAAGAGCCTT TGTTGATAAA GAAATCACTG TTAATTCATA TGTACAAGCG 
TCGTCCTCAC CAGATACAAA AGAAGAGAGC ACAAGTGATG AGCTACATGA CCAGAAAAAT 
GGTCAGGATT ATAAAAGTAG TTGTGAAGAA AAGTTAAGAG CCTTTGTTGA TAAAGAATCA 
ACTGTTAATT CGTATTTACA AGCATCGTCC TTGCCAGATT TCCATAGGAC CCTGAGGACT 
TCACCTGTAA TTGATTCACA ATGTCAGCCT ACCTTGATGG ATCCAGGGGG TGAATTGAAG 
AATGGCATAC TGCAAACAGA AGATAATTTC TGTACATTGA AGGATTGTTC TGCAGATGGA 
AATGCCAACT CTACCATTAG AAAACAATTT TATCCAGAAT CAGGTCAGTC CTCTTTTGTT 
GTTCTCACCA ATAGCTTTCC AAAGGATGCG CCGGATCTCC TTTCCAAGGG CGATGGTGCT 
TCCATCAACA ATGATTGTGC TGTAGACAAT CCTGGACAAA CAAATAATGA TGGAAAAGAA 
GATGTTGAAG TTGATCATAT TACTGAAAAC ATACTGTTAC CTTCTCAAAG GAGTCAAAGA 
ACCAAATTTG GTAGTAAGAC ACAAACCAGG AAGGCTTCTA GAAAATCCAA GAACAAAGCC 
AGTATGACAC ATCGAGGTGG AGGTATGAAT ATGAACTTAG AGGCTGCTAG AAAGAAAAGA 
AGTTGTTTAT GCAAACCGGC TCGTTCTTCT GTCTGGGGAT CGCTTGGGAA TATTGAACAG 
TTTTTTCAGC ACGAAAACGA GCTGGAGGTT AGTGAAGCCA TGTGCCAGAA TGTGGTGAAA 
GCTAGAAGCA AGTTTCAAGG TGGGGAAATG ATTAAGAATG CTACAAGTTC TAGCTCATTA 
AGTTCAGTTC AAAAGGATAA TGTTCCAACT TCAACTACTC GTTTTCGTCT GAAGATCAAG 
TTTGGAAAAG AAAATGCTTT ACGTTGCTCA AACGTCTTGA TTCCGGAGTC TGTTGGTGGC 
TTGGCTTCTG CTTCCTACTT GGAGTCGGAC CCAGGTTCTC GGAAAGTAGC CAGCAATTCT 
GCAGATAAAT TTTCTGAAGC ATTGGCTTTA AGCAATTTAG AATCATTCAG AAATGACCTG 
GACAAGGATT TTGTTGTTCT AAATGGACAA ATTGTAAATA GCCAGTTAGA AAATTCTAAA 
ATAACGGAGA AGTCAGATGA GGATGCAGTG GAACCATGTC ATGCAGGTCC TCCAGAGAAA 
GTGGTTGAAG CATTAGTTAA GCCAATTAAT AATTCAGTTA TAGATCCCGG AACGTCACCT 
GATTCAGAAG TCATTGATTC AATTCCTGAC GTCCAGGTTG GAGAAAGACA TGAAGATGTA 
CATTTTTCTG TGTTAGGTTC TTCTAAGGAG TTAAATTCTC ATATGGACGT TACCTTCAGG 
AATAGAGGGA AGAAGAAAGA CAAGCTTATT TATTCAGGCA ATTGCATCAC TGAAGATGGA 
TCACAAGGTC AACCAGGGAA TAATAGAGCT AAGCATTCGA AGAATCACAG ACGTAAGAGA 
ACCTCCAGTG ATGTGGTTAG CTCTTTAGAA TTGCCTATTT CCACTGAGCT AAGCAAATCT 
GTAAAGAGTA AAGAATTGTC TGCAGAATCA TCGCCCCTTT CTGGAGATAT TGTACTTGGA 
GGCCCTATGG AGTCTTTGAA AGTTAAAAGT CATGCGATAG TCAAGACAGC TGGCAAATCA 
TCTGCTGACC ATGGTTTTAC TGAACTCCAG GGTTCAAAAA ATTTACCGTC TTCTACAAGA 
CCTTGGGGTC GTAAACTACC TAAAAGTCTT GAACCTAGAA AAGTTAGCAA GGGTAAATCC 
AAAGCTTCTG ACTCGACAAG CAGGAAAAAG CCTACTTCTA GGCCCAAGGA GAGACAGAAA 
GAGCCAATTA ACAGAAGTGA AGTAAAGGGA AAAGATGTCT CTCTCAAAGT TACATGTGAA 
GTGGAAGATC ACCCACATCC AGATATAGTT GGAAATCATA AGTTGGCTGG TGTTGGAAAA 
ATTAATACCG GTGACAACAG AGTACCAGTC AGTGTGTCTA ATTTGGACGT GATGCCTGGT 
TTTGGTTTGG AAGAACAGCA GCAGTCACCT CGTAATGCAT GGGTGAGTTG TGATGACTGT 
CACAAGTGGC GGCGCATTCC AGCTTTGCTT GCAGACCAGA TTGATGAAAC TAACTGCACC 
TGGACTTGCA AGGACAGCAG TGATAAAGCC TATGCTGATT GTGCTATCCC TCAAGAAAAG 
TCAAATGCAG AGATTAATGC AGAGTTAGGA TTATCAGACG CCTCTGGTGA AGAAGATGCA 
TATGGAAACT CCAAAACTCA TAAGGAGTTG GAATATCAAC TGCCTTTGGT TTCCCAAGAG 
TCAACTTTTA CCCGCATTTT CACAAATGAA TTTCTGCATC GTAACCCAAG AACTCAGACT 
ATTGATGAGG TAATGGTTTG CCATTGCAAG CCACCTCGAG AAGGCAAGAT GGGATGTGGG 
GATGAATGTC TAAACCGGAT GCTTAACATC GAATGTGTGC AAGGAACTTG CCCATGTGGG 
GACCGTTGTT CCAATCAGCA GTTCCAAAAA CGCAATTATT CCAGACTGAA GTGGTTTAAA 
TGCGGGAAAA AAGGTTATGG ACTGAAGGCA CTTGAGCGTG TAGCTGAAGG CCAGTTTATT 
ATTGAATACG TTGGAGAGGT GCTTGACGTG CATGCATACG AGGCACGCCA GAGAGAGTAT 
GCTTTGAAGG GTCATAGACA TTTCTATTTT ATGACCTTGA ACGGCAGTGA GGTGATAGAT 
GCAAGTGCAA AAGGAAATTT GGGGCGTTTC ATTAATCATA GTTGTGATCC TAATTGTCGG 
ACAGAAAAGT GGATGGTGAA TGGAGAAATC TGTATTGGAC TGTTTGCATT GAGGAATATC 
AAGCAGGATG AAGAATTGAC ATTTGATTAC AACTATGTAA GGGTTTTTGG TGCTGCTGCC 
AAAAAGTGTT ATTGTGGTTC ACTTCACTGT CAAGGATATA TAGGCGGTGC GGATCCAAAT 
AACGGTGAGC TTATAGTTCA AGGTGAATCA GATGATGAAT TTCCAGAACC TATGATGCTT 
TCTGAAAATG GTGAAATTGA CGACAGCGTA CTCATGCCCA AATGCATTGA TAGTGTTAAT 
ACAAAGTCTT CTAGGCATTT AATAACAGAC AGGGATGTAT TGGACAAGTG TACAACTGCT 
ATATGTGCAG ATGGTTCTCC AGAGGAAGAC AGTTCTACAA ACCCTGCCTC TGCTGTTTCC 
CTGTTGCACA GTTCAGTTGA AGTGGAAGAC TCAAAAAGCA ATTTACCATC TTCTGATCGA 
ATAGAAGAAA TTTCTCAACA AATAGAGGAT ACAACAAGCA AACCCATGCC TGCTGACAGC 
AAAGAATTGC CGAATTCTAC TGATAGTAAT AGAGAATCCA AATCTGAAAT GGTTGAAGTC 
GGGAATGATT TTTCACAATC ACATCTGCTT GTGAAGACTC CTCGACTAAA TGCTTCTGTT 
AAAAAGGGTA AGGTAAGGGC TAATGCTGCA AATGCCCTTA CAGCCGAGGT GGCGGCCCCT 
CGATTGCCAG TATCATCTAT CAAAAACAAA AAAGTAGAAG GTTCTTCCAA TGGACGGTTT 
GAAGCAGTTC AGGGGAAACT TAATGAGTTG CTGGATGGAA ATGGAGGAAT AAGCAAAAGA 
AAAGATGCCA CCAAAGGCTA CTTGAAACTT TTGCTTCTCA CCGTGGCATC AGGCGATAGA 
AGCAATGGTG AAGCAATTCA GAGTAACCGA GATCTTTCCA TGATCCTTGA TGCTCTTCTG 
AAAACAAAAT CGAGAGCCGT GCTAAATGAT ATTATTAGTA AAAATGGTTT ACAGATGTTA 
CATAAGATCA TGAAGCAGTA TAGGCAAGAC TTCAAAAAAA TTCCAATACT ACGAAAGCTT 
CTTAAGGTCT TAGAGTACCT GGCAGCAGGC AAGATTTTGA CTCCTGAACA TATCAATGGT 
GGTCCTCCTT GTCATGGAAT GGAGAGGTTT AGGGATTCAA TGCTGTCTCT GACAGAGCAT 
GATGATAAAC AGGTCCATCA AATTGCTCGA AGCTTCCGAG ACAGATGGAT TCCAAGACAT 
GGCAGAAAAC ATGGCTATAT GGACAGGGAT GATAACAGGA TGGAATCTCA CAGAGGTTTC 
AACTCCAACA GATTTTCAGT ATCACACAGT CATAGGCACG AGCAGGGATT AAGACCTAAG 
GAAGCAACTG ATTGTGGTCA GCAGCCAATG CTTGTGGCAA CAGTAGACGC GCGTGCCCAG 
GAGGGCTGCT CTACACCATC TTTGGATGGA GTTGAGATCA ACGGGGCAAA AAAACGTAAG 
CGCAAAAGCC GATGGGATCA ACCAGCAGAA ACAAATTCAT ATTCTGATGC TATCATTAGT 
TCTATTAATG AAAGCCAGAA CGTTCATGAA GAAGTTCCAC CCGGATTCTC ATGTCCAATA 
CGTTCATTAA ACAGTGCACT AAACTCTGGT ACTCCTGCTT TGCAAAATGC AAGCCATTCT 
GGATGCCCCC CAAGTTTAGT TATTGGTCAG CCAAAAGAGA AATTCAATTC TCGTTTGCCT 
GTCTCATATG GATTGCCATG GTCTGTTGCG CAACAATATG GAACTCCTCA TGCTGAAATC 
ACAGGGTGTT GGATCACTGC TCCTGGCATG CCTTTCAACC CGTTTCCTCC ATTACCCCCA 
TATCCACGGG ATAACAAAGA CTGTCAACCT TCCAGTATGG AAATTGATCA GCCTGCTGAA 
GTTAAGCAAT CGGACGCCAC TGGTCCGGTT AATTGCTGCT CAGATGATAT GATTCCCAGC 
ACAACTGGTG CTAACTCTGA AGACACGAAC CTTCAATGTG AGGATGCTAA ACATGACGCT 
AAGCGATTGA AGGGTGATGA TTCTGATGAT TTGGGAAAGA AGTACTTTAG ACAGCAGAAA 
TGGAATAATT CAAAAATACA TCGAACATGG TTCAAAAGGG ATGCATGGAA ATGTAATGGG 
AACAGCTCTA GTGGTGATAT TTGCAGTATA GATGTAGGAG ATGTGTCAAA AGAATCAAAA 
GTTACTAGTT ATTCAGAGGA GGATGAAATC TGTAGAGAGG AGAAAGGTGG AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004502541.1
CDS	372..6308
Translation	MGSCGEPVIVGDPAQLEQPLCSGFPAQLVSVQESCSEEPCDVLDVSGMADGFLGEGEVGCSASYLDVMEGYGDALTNECENDDMSQLEKTAEDGCWNSLGICSEDIEVSGLKSGGLCLEGNFKDEGNLDLPLESITSNDLQKHWAEQGEQKNGKNIMVFSVGDDLTVVEGKNDGADLLAGAVNCVLDVEHSEVPLESDSWPKAVENCDALDGIEANSCRQISSSLNFEVPSGVLFIDAKVESTSDKLHDLKNGEDCDSTCDEKIRAFVDKEITVNSYVQASSSPDTKEESTSDELHDQKNGQDYKSSCEEKLRAFVDKESTVNSYLQASSLPDFHRTLRTSPVIDSQCQPTLMDPGGELKNGILQTEDNFCTLKDCSADGNANSTIRKQFYPESGQSSFVVLTNSFPKDAPDLLSKGDGASINNDCAVDNPGQTNNDGKEDVEVDHITENILLPSQRSQRTKFGSKTQTRKASRKSKNKASMTHRGGGMNMNLEAARKKRSCLCKPARSSVWGSLGNIEQFFQHENELEVSEAMCQNVVKARSKFQGGEMIKNATSSSSLSSVQKDNVPTSTTRFRLKIKFGKENALRCSNVLIPESVGGLASASYLESDPGSRKVASNSADKFSEALALSNLESFRNDLDKDFVVLNGQIVNSQLENSKITEKSDEDAVEPCHAGPPEKVVEALVKPINNSVIDPGTSPDSEVIDSIPDVQVGERHEDVHFSVLGSSKELNSHMDVTFRNRGKKKDKLIYSGNCITEDGSQGQPGNNRAKHSKNHRRKRTSSDVVSSLELPISTELSKSVKSKELSAESSPLSGDIVLGGPMESLKVKSHAIVKTAGKSSADHGFTELQGSKNLPSSTRPWGRKLPKSLEPRKVSKGKSKASDSTSRKKPTSRPKERQKEPINRSEVKGKDVSLKVTCEVEDHPHPDIVGNHKLAGVGKINTGDNRVPVSVSNLDVMPGFGLEEQQQSPRNAWVSCDDCHKWRRIPALLADQIDETNCTWTCKDSSDKAYADCAIPQEKSNAEINAELGLSDASGEEDAYGNSKTHKELEYQLPLVSQESTFTRIFTNEFLHRNPRTQTIDEVMVCHCKPPREGKMGCGDECLNRMLNIECVQGTCPCGDRCSNQQFQKRNYSRLKWFKCGKKGYGLKALERVAEGQFIIEYVGEVLDVHAYEARQREYALKGHRHFYFMTLNGSEVIDASAKGNLGRFINHSCDPNCRTEKWMVNGEICIGLFALRNIKQDEELTFDYNYVRVFGAAAKKCYCGSLHCQGYIGGADPNNGELIVQGESDDEFPEPMMLSENGEIDDSVLMPKCIDSVNTKSSRHLITDRDVLDKCTTAICADGSPEEDSSTNPASAVSLLHSSVEVEDSKSNLPSSDRIEEISQQIEDTTSKPMPADSKELPNSTDSNRESKSEMVEVGNDFSQSHLLVKTPRLNASVKKGKVRANAANALTAEVAAPRLPVSSIKNKKVEGSSNGRFEAVQGKLNELLDGNGGISKRKDATKGYLKLLLLTVASGDRSNGEAIQSNRDLSMILDALLKTKSRAVLNDIISKNGLQMLHKIMKQYRQDFKKIPILRKLLKVLEYLAAGKILTPEHINGGPPCHGMERFRDSMLSLTEHDDKQVHQIARSFRDRWIPRHGRKHGYMDRDDNRMESHRGFNSNRFSVSHSHRHEQGLRPKEATDCGQQPMLVATVDARAQEGCSTPSLDGVEINGAKKRKRKSRWDQPAETNSYSDAIISSINESQNVHEEVPPGFSCPIRSLNSALNSGTPALQNASHSGCPPSLVIGQPKEKFNSRLPVSYGLPWSVAQQYGTPHAEITGCWITAPGMPFNPFPPLPPYPRDNKDCQPSSMEIDQPAEVKQSDATGPVNCCSDDMIPSTTGANSEDTNLQCEDAKHDAKRLKGDDSDDLGKKYFRQQKWNNSKIHRTWFKRDAWKCNGNSSSGDICSIDVGDVSKESKVTSYSEEDEICREEKGGK

Target ORF information:

RefSeq Version	XM_004502484.3
Organism	Cicer arietinum(chickpea)
Definition	Cicer arietinum histone-lysine N-methyltransferase ASHH2 (LOC101503412), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004502484.3

ORF Insert Sequence:

ATGGGCTCGT GTGGTGAACC GGTAATCGTT GGTGATCCGG CACAATTGGA GCAGCCTTTG 
TGCTCGGGTT TTCCGGCACA GTTAGTTTCT GTGCAGGAGT CATGTTCAGA AGAACCTTGC 
GATGTTTTAG ACGTATCTGG TATGGCGGAT GGTTTTTTGG GAGAGGGTGA AGTGGGTTGT 
TCTGCAAGCT ATCTTGATGT TATGGAGGGG TACGGAGATG CTTTGACAAA TGAATGTGAA 
AATGATGATA TGTCACAATT GGAGAAAACT GCTGAAGATG GTTGTTGGAA TAGTTTGGGG 
ATTTGTTCTG AGGACATTGA AGTTTCCGGT CTTAAAAGTG GTGGATTGTG CTTAGAAGGA 
AATTTTAAAG ATGAAGGAAA CCTTGACCTG CCGTTGGAGT CCATAACCTC AAATGATTTG 
CAGAAGCATT GGGCTGAACA GGGTGAGCAG AAGAATGGCA AGAACATTAT GGTATTCTCG 
GTAGGAGATG ATTTGACAGT CGTGGAAGGC AAAAACGATG GTGCTGATTT ACTAGCTGGT 
GCTGTCAACT GTGTGCTTGA TGTTGAGCAT TCTGAAGTGC CTTTGGAATC AGATAGCTGG 
CCAAAGGCTG TGGAAAATTG TGATGCTTTA GATGGGATAG AAGCGAATTC TTGCAGGCAG 
ATATCTTCTT CACTAAATTT TGAAGTGCCG TCAGGTGTCT TGTTTATAGA TGCAAAAGTA 
GAGAGCACAA GTGATAAGCT ACACGATCTA AAAAATGGCG AGGATTGTGA CAGTACTTGT 
GATGAAAAGA TAAGAGCCTT TGTTGATAAA GAAATCACTG TTAATTCATA TGTACAAGCG 
TCGTCCTCAC CAGATACAAA AGAAGAGAGC ACAAGTGATG AGCTACATGA CCAGAAAAAT 
GGTCAGGATT ATAAAAGTAG TTGTGAAGAA AAGTTAAGAG CCTTTGTTGA TAAAGAATCA 
ACTGTTAATT CGTATTTACA AGCATCGTCC TTGCCAGATT TCCATAGGAC CCTGAGGACT 
TCACCTGTAA TTGATTCACA ATGTCAGCCT ACCTTGATGG ATCCAGGGGG TGAATTGAAG 
AATGGCATAC TGCAAACAGA AGATAATTTC TGTACATTGA AGGATTGTTC TGCAGATGGA 
AATGCCAACT CTACCATTAG AAAACAATTT TATCCAGAAT CAGGTCAGTC CTCTTTTGTT 
GTTCTCACCA ATAGCTTTCC AAAGGATGCG CCGGATCTCC TTTCCAAGGG CGATGGTGCT 
TCCATCAACA ATGATTGTGC TGTAGACAAT CCTGGACAAA CAAATAATGA TGGAAAAGAA 
GATGTTGAAG TTGATCATAT TACTGAAAAC ATACTGTTAC CTTCTCAAAG GAGTCAAAGA 
ACCAAATTTG GTAGTAAGAC ACAAACCAGG AAGGCTTCTA GAAAATCCAA GAACAAAGCC 
AGTATGACAC ATCGAGGTGG AGGTATGAAT ATGAACTTAG AGGCTGCTAG AAAGAAAAGA 
AGTTGTTTAT GCAAACCGGC TCGTTCTTCT GTCTGGGGAT CGCTTGGGAA TATTGAACAG 
TTTTTTCAGC ACGAAAACGA GCTGGAGGTT AGTGAAGCCA TGTGCCAGAA TGTGGTGAAA 
GCTAGAAGCA AGTTTCAAGG TGGGGAAATG ATTAAGAATG CTACAAGTTC TAGCTCATTA 
AGTTCAGTTC AAAAGGATAA TGTTCCAACT TCAACTACTC GTTTTCGTCT GAAGATCAAG 
TTTGGAAAAG AAAATGCTTT ACGTTGCTCA AACGTCTTGA TTCCGGAGTC TGTTGGTGGC 
TTGGCTTCTG CTTCCTACTT GGAGTCGGAC CCAGGTTCTC GGAAAGTAGC CAGCAATTCT 
GCAGATAAAT TTTCTGAAGC ATTGGCTTTA AGCAATTTAG AATCATTCAG AAATGACCTG 
GACAAGGATT TTGTTGTTCT AAATGGACAA ATTGTAAATA GCCAGTTAGA AAATTCTAAA 
ATAACGGAGA AGTCAGATGA GGATGCAGTG GAACCATGTC ATGCAGGTCC TCCAGAGAAA 
GTGGTTGAAG CATTAGTTAA GCCAATTAAT AATTCAGTTA TAGATCCCGG AACGTCACCT 
GATTCAGAAG TCATTGATTC AATTCCTGAC GTCCAGGTTG GAGAAAGACA TGAAGATGTA 
CATTTTTCTG TGTTAGGTTC TTCTAAGGAG TTAAATTCTC ATATGGACGT TACCTTCAGG 
AATAGAGGGA AGAAGAAAGA CAAGCTTATT TATTCAGGCA ATTGCATCAC TGAAGATGGA 
TCACAAGGTC AACCAGGGAA TAATAGAGCT AAGCATTCGA AGAATCACAG ACGTAAGAGA 
ACCTCCAGTG ATGTGGTTAG CTCTTTAGAA TTGCCTATTT CCACTGAGCT AAGCAAATCT 
GTAAAGAGTA AAGAATTGTC TGCAGAATCA TCGCCCCTTT CTGGAGATAT TGTACTTGGA 
GGCCCTATGG AGTCTTTGAA AGTTAAAAGT CATGCGATAG TCAAGACAGC TGGCAAATCA 
TCTGCTGACC ATGGTTTTAC TGAACTCCAG GGTTCAAAAA ATTTACCGTC TTCTACAAGA 
CCTTGGGGTC GTAAACTACC TAAAAGTCTT GAACCTAGAA AAGTTAGCAA GGGTAAATCC 
AAAGCTTCTG ACTCGACAAG CAGGAAAAAG CCTACTTCTA GGCCCAAGGA GAGACAGAAA 
GAGCCAATTA ACAGAAGTGA AGTAAAGGGA AAAGATGTCT CTCTCAAAGT TACATGTGAA 
GTGGAAGATC ACCCACATCC AGATATAGTT GGAAATCATA AGTTGGCTGG TGTTGGAAAA 
ATTAATACCG GTGACAACAG AGTACCAGTC AGTGTGTCTA ATTTGGACGT GATGCCTGGT 
TTTGGTTTGG AAGAACAGCA GCAGTCACCT CGTAATGCAT GGGTGAGTTG TGATGACTGT 
CACAAGTGGC GGCGCATTCC AGCTTTGCTT GCAGACCAGA TTGATGAAAC TAACTGCACC 
TGGACTTGCA AGGACAGCAG TGATAAAGCC TATGCTGATT GTGCTATCCC TCAAGAAAAG 
TCAAATGCAG AGATTAATGC AGAGTTAGGA TTATCAGACG CCTCTGGTGA AGAAGATGCA 
TATGGAAACT CCAAAACTCA TAAGGAGTTG GAATATCAAC TGCCTTTGGT TTCCCAAGAG 
TCAACTTTTA CCCGCATTTT CACAAATGAA TTTCTGCATC GTAACCCAAG AACTCAGACT 
ATTGATGAGG TAATGGTTTG CCATTGCAAG CCACCTCGAG AAGGCAAGAT GGGATGTGGG 
GATGAATGTC TAAACCGGAT GCTTAACATC GAATGTGTGC AAGGAACTTG CCCATGTGGG 
GACCGTTGTT CCAATCAGCA GTTCCAAAAA CGCAATTATT CCAGACTGAA GTGGTTTAAA 
TGCGGGAAAA AAGGTTATGG ACTGAAGGCA CTTGAGCGTG TAGCTGAAGG CCAGTTTATT 
ATTGAATACG TTGGAGAGGT GCTTGACGTG CATGCATACG AGGCACGCCA GAGAGAGTAT 
GCTTTGAAGG GTCATAGACA TTTCTATTTT ATGACCTTGA ACGGCAGTGA GGTGATAGAT 
GCAAGTGCAA AAGGAAATTT GGGGCGTTTC ATTAATCATA GTTGTGATCC TAATTGTCGG 
ACAGAAAAGT GGATGGTGAA TGGAGAAATC TGTATTGGAC TGTTTGCATT GAGGAATATC 
AAGCAGGATG AAGAATTGAC ATTTGATTAC AACTATGTAA GGGTTTTTGG TGCTGCTGCC 
AAAAAGTGTT ATTGTGGTTC ACTTCACTGT CAAGGATATA TAGGCGGTGC GGATCCAAAT 
AACGGTGAGC TTATAGTTCA AGGTGAATCA GATGATGAAT TTCCAGAACC TATGATGCTT 
TCTGAAAATG GTGAAATTGA CGACAGCGTA CTCATGCCCA AATGCATTGA TAGTGTTAAT 
ACAAAGTCTT CTAGGCATTT AATAACAGAC AGGGATGTAT TGGACAAGTG TACAACTGCT 
ATATGTGCAG ATGGTTCTCC AGAGGAAGAC AGTTCTACAA ACCCTGCCTC TGCTGTTTCC 
CTGTTGCACA GTTCAGTTGA AGTGGAAGAC TCAAAAAGCA ATTTACCATC TTCTGATCGA 
ATAGAAGAAA TTTCTCAACA AATAGAGGAT ACAACAAGCA AACCCATGCC TGCTGACAGC 
AAAGAATTGC CGAATTCTAC TGATAGTAAT AGAGAATCCA AATCTGAAAT GGTTGAAGTC 
GGGAATGATT TTTCACAATC ACATCTGCTT GTGAAGACTC CTCGACTAAA TGCTTCTGTT 
AAAAAGGGTA AGGTAAGGGC TAATGCTGCA AATGCCCTTA CAGCCGAGGT GGCGGCCCCT 
CGATTGCCAG TATCATCTAT CAAAAACAAA AAAGTAGAAG GTTCTTCCAA TGGACGGTTT 
GAAGCAGTTC AGGGGAAACT TAATGAGTTG CTGGATGGAA ATGGAGGAAT AAGCAAAAGA 
AAAGATGCCA CCAAAGGCTA CTTGAAACTT TTGCTTCTCA CCGTGGCATC AGGCGATAGA 
AGCAATGGTG AAGCAATTCA GAGTAACCGA GATCTTTCCA TGATCCTTGA TGCTCTTCTG 
AAAACAAAAT CGAGAGCCGT GCTAAATGAT ATTATTAGTA AAAATGGTTT ACAGATGTTA 
CATAAGATCA TGAAGCAGTA TAGGCAAGAC TTCAAAAAAA TTCCAATACT ACGAAAGCTT 
CTTAAGGTCT TAGAGTACCT GGCAGCAGGC AAGATTTTGA CTCCTGAACA TATCAATGGT 
GGTCCTCCTT GTCATGGAAT GGAGAGGTTT AGGGATTCAA TGCTGTCTCT GACAGAGCAT 
GATGATAAAC AGGTCCATCA AATTGCTCGA AGCTTCCGAG ACAGATGGAT TCCAAGACAT 
GGCAGAAAAC ATGGCTATAT GGACAGGGAT GATAACAGGA TGGAATCTCA CAGAGGTTTC 
AACTCCAACA GATTTTCAGT ATCACACAGT CATAGGCACG AGCAGGGATT AAGACCTAAG 
GAAGCAACTG ATTGTGGTCA GCAGCCAATG CTTGTGGCAA CAGTAGACGC GCGTGCCCAG 
GAGGGCTGCT CTACACCATC TTTGGATGGA GTTGAGATCA ACGGGGCAAA AAAACGTAAG 
CGCAAAAGCC GATGGGATCA ACCAGCAGAA ACAAATTCAT ATTCTGATGC TATCATTAGT 
TCTATTAATG AAAGCCAGAA CGTTCATGAA GAAGTTCCAC CCGGATTCTC ATGTCCAATA 
CGTTCATTAA ACAGTGCACT AAACTCTGGT ACTCCTGCTT TGCAAAATGC AAGCCATTCT 
GGATGCCCCC CAAGTTTAGT TATTGGTCAG CCAAAAGAGA AATTCAATTC TCGTTTGCCT 
GTCTCATATG GATTGCCATG GTCTGTTGCG CAACAATATG GAACTCCTCA TGCTGAAATC 
ACAGGGTGTT GGATCACTGC TCCTGGCATG CCTTTCAACC CGTTTCCTCC ATTACCCCCA 
TATCCACGGG ATAACAAAGA CTGTCAACCT TCCAGTATGG AAATTGATCA GCCTGCTGAA 
GTTAAGCAAT CGGACGCCAC TGGTCCGGTT AATTGCTGCT CAGATGATAT GATTCCCAGC 
ACAACTGGTG CTAACTCTGA AGACACGAAC CTTCAATGTG AGGATGCTAA ACATGACGCT 
AAGCGATTGA AGGGTGATGA TTCTGATGAT TTGGGAAAGA AGTACTTTAG ACAGCAGAAA 
TGGAATAATT CAAAAATACA TCGAACATGG TTCAAAAGGG ATGCATGGAA ATGTAATGGG 
AACAGCTCTA GTGGTGATAT TTGCAGTATA GATGTAGGAG ATGTGTCAAA AGAATCAAAA 
GTTACTAGTT ATTCAGAGGA GGATGAAATC TGTAGAGAGG AGAAAGGTGG AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC101503412 cDNA ORF clone, Cicer arietinum(chickpea)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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