ORF » Species Summary » Cyprinodon variegatus » vcl cDNA ORF clone

vcl cDNA ORF clone, Cyprinodon variegatus(sheepshead minnow)

The following vcl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the vcl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCy01394 XM_015382261.1
Latest version!		 Cyprinodon variegatus vinculin (vcl), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCy01395 XM_015382269.1
Latest version!		 Cyprinodon variegatus vinculin (vcl), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    vcl ( XM_015382261.1 ) cDNA ORF clone, Cyprinodon variegatus(sheepshead minnow) -> XP_015237747.1 Cyprinodon variegatus vinculin (vcl), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OCy01394
    Clone ID Related Accession (Same CDS sequence) XM_015382261.1
    Accession Version XM_015382261.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3204bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-13
    Organism Cyprinodon variegatus(sheepshead minnow)
    Product vinculin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015150465.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cyprinodon variegatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCCGGTTT TCCACACGAA GACCATAGAA AGTATCCTGG AGCCGGTGGC TCAGCAGATA 
    TCCCATCTGG TAATAATGCA CGAGGAAGGT GAAGTTGACG GAAAGGCTAT CCCGGACCTG 
    TCTGCGCCGG TGGCTGCTGT TCAGGCGGCC GTTAGCAACC TCGTTAGGGT TGGAAAGGAT 
    ACTGTGCAAA CAACAGAGGA CCAGATCATG AAAAGGGATA TGCCCCCAGC CTTTATCAAA 
    GTGGAGAATG CCTGTACAAA GCTGGTTCAA GCTGCTTCCA TGCTGAAAGC CGACCCATAC 
    TCTGTTCCGG CTCGGGATTA CCTCATCGAT GGTTCCCGGG GCATTCTGTC TGGGACGTCT 
    GACCTGCTCC TGACTTTTGA TGAGGCAGAG GTCCGCAAAA TAATCCGTGT ATGCAAAGGC 
    ATCTTGGAGT ATCTGAGTGT GGCAGAGGTG GTGGAGTCCA TGGAGGATCT GATCACATAC 
    ACAAAGAATC TGGGACCAGG GATGACCAAG ATGGCGAAGA TGATAGATGA GCGCCAGCAG 
    GAGCTGACAC ATCAGGAACA TCGTGTGATG CTTGTTAACT CGATGAACAC GGTCAAAGAG 
    CTGCTGCCCA TCCTCATATC AGGTATAAAA ATCTTTGTTA CAACCAAGAC ATCTGGGAGC 
    CAGGGTGTCG AGGAGGCCCT GAAGAACCGA AACTTCACGT TTGAGAAGAT GAGTGCTGAG 
    ATAAATGAGA TCATCCGAGT GCTGCAGCTT ACATCCTGGG ATGAAGATGC TTGGGCCAAT 
    AAGAAGGACA CAGAGGCCAT GAAGAGGGCT CTAGGACTGA TCGACTCCAA AATGGGTCAG 
    GCTAAAAACT GGCTGAGGGA TCCAAATGCT CCACCAGGGG AGGCTGGCGA GCAGGCCGTC 
    CGGCAGATCC TGGATGAAGC TGGTAAAGTT GGTGAGCTGT GTGCCGGGAA GGAGCGCAGA 
    GACATCCTGG GCACGGCCAA GACTCTGGGC CAGATGACTG ATCAGGTGTC TGAGATGAGG 
    GCGAGAGGTC AGGGAGCATC TCCGGCTGCC ATGCAGAAAG CGCAGCAGGT TTCCCATGGC 
    CTGGATGTGC TCACTGGCAA GGTTGAAAAC GCCGCTCGCA AACTCGAGGC CATGACGAAC 
    TCCAAGCAGG CCATCGCCAA GAGGATCGAT GCTGCACAGA GCTGGCTCGC AGACCCCCAC 
    GGTGGCCCAG AGGGAGAGGA GAATATCAAG GCCCTCCTGG GTGAGGCCAA AAAGATTGCG 
    GATATGTGCG AGGATCCCAA AGAGCGGGAA GAGATTTTGC GCTCTGCTGG AGAGCTGGCT 
    GCCATGACGG CCAAACTGTC TGAGCTCAGA AGGCAGGGTA AAGGTGACAC TCCTGAGGCC 
    CGAGCCTTAG CCAAGCAGAT AGCAACAGCC CTGCAGAACC TGCAGTCCAA GACCAACAAG 
    GCTGTAGCCA ACAGCAGGCC TGCAAAGGCA GCCGTTCACT TGGAGGGGAA GACGGAGCAG 
    GCGCAGCGCT GGATCGACAA CCCTACTGTG GACGACAGTG GTGTAGGCCA GGCAGCCATC 
    CGTGGCCTGG TGGCAGAGGG CCGTCGGCTA GCCAATGCTC TACAGGGTCC GTACAGGCAG 
    GAGCTGATGG GCAAGTGTGA GCAAGTGGAG CAGCTCATGG CCCAGCTGGC TGACCTGGCT 
    GCCCGTGGCG AAGGGGACTC CCCGCAAGCA CGTGCTGTAG CTCAACAGCT CCAAGAGACC 
    TTGAAAGACC TGAAAGGAAA GATGCAGGAG GCCATGACTC AGGAGGTTTC TGACATCTTC 
    AGTGACACCA CCACCCCTAT CAAGTTACTG GCAGTGGCTG CTACAGCCCC CCTTGATGCA 
    CCCAACAGAG ATGAGGTCTT TGATGATAGA GCCAATAACT TCGAGAACCA TGCCAACAGG 
    CTTGGTGCTA CGGCCGAGAA GGCAGCCGCA GTCGGCACAG CAAACAAGAA CACGGTGGAG 
    GGAATCCAGG CTGCCGTCAA GTCAACCAGA GACCTAACAC CACAAGTGGT CTCTGCTGCT 
    CGTATTCTGC TGAGAAACCC TGGCAACCAA GCAGCGTATG AACATTTTGA GACCATGAAG 
    AATCAGTGGA TTGACAACGT GGAAAAAATG ACCGGTTTGG TGGATGAGGC CATCGATACC 
    AAATCTCTGC TGGACGCCTC TGAGGACGCC ATAAAGAAGG ACTTGGATAA ATGCCGCGTG 
    GCCATGACCA ATCACCAGCC CCAGATGCTG GTGGCCGGTG CTACGAGCAT TGCCCGAAGA 
    GCCAACCGCA TCCTTCTGGT GGCGAAACGA GAGGTGGAGA ACTCTGAGGA CCCAAAATTC 
    AGGGAAATGG TTAAAGCTGC GTCGGATGAA CTGAGCCAGA CTATTTCTCC CATGGTGATG 
    AATGCAAAAG CTGTGGCCGG AAACATCCAA GACCCCAACC TTCAGAAGAG CTTTTTGGAC 
    TCGGGTTTTA AGATTCTTGG AGCTGTGGCA AAGGTCAGGG AAGCCTTCCA GCCCCAAGAG 
    CCAGACTTCC CACCACCTCC TCCTGAGTTG GATCAGCTCA ATCTCAATGA TGAAGCGGCA 
    CCTCCAAAGC CTCCTCTTCC AGAGGGAGAG GTTCCTCCAC CCAGGCCTCC TCCTCCTGAG 
    GAGAAAGACG AGGAGTTCCC AGAGCACAAA GCTGGAGAAA AAGTCAATGA GCCCATAATG 
    GTGGCTGCCA GGCAGCTTCA CGACGAAGCC CGCAAATGGT CTAGCAAAGG AAACGACATA 
    ATTGGTGCTG CCAAACGAAT GGCCCTGCTC ATGGCAGAAA TGTCTCGTTT GGTGCGTGGA 
    GGCAGCGGAA ACAAGCGTGC TCTGATCCAG TGCGCCAAGG ACATCGCCAA AGCCTCCGAC 
    GAAGTCACAC GACTAGCTAA GGAAGTGGCC AAGCAGTGTA CCGACAAACG GATCCGGACC 
    AACCTCCTCC AAGTGTGTGA GCGCATTCCC ACCATCAGCA CTCAGCTCAA AATCCTTTCC 
    ACTGTCAAGG CCACCATGTT GGGCCGCACC AACATCAGTG AAGAAGAGTC GGAGCAGGCG 
    ACTGAAATGT TGGTTCACAA CGCCCAGAAC CTGATGCAAT CAGTAAAGGA GACCGTCAGA 
    GAGGCAGAGG CAGCTTCCAT TAAGATCCGG ACAGATGCAG GTTGCACCCT CCACTGGGTC 
    AGAAAGACCC CCTGGTACCA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015237747.1
    CDS164..3367
    Translation

    Target ORF information:

    RefSeq Version XM_015382261.1
    Organism Cyprinodon variegatus(sheepshead minnow)
    Definition Cyprinodon variegatus vinculin (vcl), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_015382261.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCCGGTTT TCCACACGAA GACCATAGAA AGTATCCTGG AGCCGGTGGC TCAGCAGATA 
    TCCCATCTGG TAATAATGCA CGAGGAAGGT GAAGTTGACG GAAAGGCTAT CCCGGACCTG 
    TCTGCGCCGG TGGCTGCTGT TCAGGCGGCC GTTAGCAACC TCGTTAGGGT TGGAAAGGAT 
    ACTGTGCAAA CAACAGAGGA CCAGATCATG AAAAGGGATA TGCCCCCAGC CTTTATCAAA 
    GTGGAGAATG CCTGTACAAA GCTGGTTCAA GCTGCTTCCA TGCTGAAAGC CGACCCATAC 
    TCTGTTCCGG CTCGGGATTA CCTCATCGAT GGTTCCCGGG GCATTCTGTC TGGGACGTCT 
    GACCTGCTCC TGACTTTTGA TGAGGCAGAG GTCCGCAAAA TAATCCGTGT ATGCAAAGGC 
    ATCTTGGAGT ATCTGAGTGT GGCAGAGGTG GTGGAGTCCA TGGAGGATCT GATCACATAC 
    ACAAAGAATC TGGGACCAGG GATGACCAAG ATGGCGAAGA TGATAGATGA GCGCCAGCAG 
    GAGCTGACAC ATCAGGAACA TCGTGTGATG CTTGTTAACT CGATGAACAC GGTCAAAGAG 
    CTGCTGCCCA TCCTCATATC AGGTATAAAA ATCTTTGTTA CAACCAAGAC ATCTGGGAGC 
    CAGGGTGTCG AGGAGGCCCT GAAGAACCGA AACTTCACGT TTGAGAAGAT GAGTGCTGAG 
    ATAAATGAGA TCATCCGAGT GCTGCAGCTT ACATCCTGGG ATGAAGATGC TTGGGCCAAT 
    AAGAAGGACA CAGAGGCCAT GAAGAGGGCT CTAGGACTGA TCGACTCCAA AATGGGTCAG 
    GCTAAAAACT GGCTGAGGGA TCCAAATGCT CCACCAGGGG AGGCTGGCGA GCAGGCCGTC 
    CGGCAGATCC TGGATGAAGC TGGTAAAGTT GGTGAGCTGT GTGCCGGGAA GGAGCGCAGA 
    GACATCCTGG GCACGGCCAA GACTCTGGGC CAGATGACTG ATCAGGTGTC TGAGATGAGG 
    GCGAGAGGTC AGGGAGCATC TCCGGCTGCC ATGCAGAAAG CGCAGCAGGT TTCCCATGGC 
    CTGGATGTGC TCACTGGCAA GGTTGAAAAC GCCGCTCGCA AACTCGAGGC CATGACGAAC 
    TCCAAGCAGG CCATCGCCAA GAGGATCGAT GCTGCACAGA GCTGGCTCGC AGACCCCCAC 
    GGTGGCCCAG AGGGAGAGGA GAATATCAAG GCCCTCCTGG GTGAGGCCAA AAAGATTGCG 
    GATATGTGCG AGGATCCCAA AGAGCGGGAA GAGATTTTGC GCTCTGCTGG AGAGCTGGCT 
    GCCATGACGG CCAAACTGTC TGAGCTCAGA AGGCAGGGTA AAGGTGACAC TCCTGAGGCC 
    CGAGCCTTAG CCAAGCAGAT AGCAACAGCC CTGCAGAACC TGCAGTCCAA GACCAACAAG 
    GCTGTAGCCA ACAGCAGGCC TGCAAAGGCA GCCGTTCACT TGGAGGGGAA GACGGAGCAG 
    GCGCAGCGCT GGATCGACAA CCCTACTGTG GACGACAGTG GTGTAGGCCA GGCAGCCATC 
    CGTGGCCTGG TGGCAGAGGG CCGTCGGCTA GCCAATGCTC TACAGGGTCC GTACAGGCAG 
    GAGCTGATGG GCAAGTGTGA GCAAGTGGAG CAGCTCATGG CCCAGCTGGC TGACCTGGCT 
    GCCCGTGGCG AAGGGGACTC CCCGCAAGCA CGTGCTGTAG CTCAACAGCT CCAAGAGACC 
    TTGAAAGACC TGAAAGGAAA GATGCAGGAG GCCATGACTC AGGAGGTTTC TGACATCTTC 
    AGTGACACCA CCACCCCTAT CAAGTTACTG GCAGTGGCTG CTACAGCCCC CCTTGATGCA 
    CCCAACAGAG ATGAGGTCTT TGATGATAGA GCCAATAACT TCGAGAACCA TGCCAACAGG 
    CTTGGTGCTA CGGCCGAGAA GGCAGCCGCA GTCGGCACAG CAAACAAGAA CACGGTGGAG 
    GGAATCCAGG CTGCCGTCAA GTCAACCAGA GACCTAACAC CACAAGTGGT CTCTGCTGCT 
    CGTATTCTGC TGAGAAACCC TGGCAACCAA GCAGCGTATG AACATTTTGA GACCATGAAG 
    AATCAGTGGA TTGACAACGT GGAAAAAATG ACCGGTTTGG TGGATGAGGC CATCGATACC 
    AAATCTCTGC TGGACGCCTC TGAGGACGCC ATAAAGAAGG ACTTGGATAA ATGCCGCGTG 
    GCCATGACCA ATCACCAGCC CCAGATGCTG GTGGCCGGTG CTACGAGCAT TGCCCGAAGA 
    GCCAACCGCA TCCTTCTGGT GGCGAAACGA GAGGTGGAGA ACTCTGAGGA CCCAAAATTC 
    AGGGAAATGG TTAAAGCTGC GTCGGATGAA CTGAGCCAGA CTATTTCTCC CATGGTGATG 
    AATGCAAAAG CTGTGGCCGG AAACATCCAA GACCCCAACC TTCAGAAGAG CTTTTTGGAC 
    TCGGGTTTTA AGATTCTTGG AGCTGTGGCA AAGGTCAGGG AAGCCTTCCA GCCCCAAGAG 
    CCAGACTTCC CACCACCTCC TCCTGAGTTG GATCAGCTCA ATCTCAATGA TGAAGCGGCA 
    CCTCCAAAGC CTCCTCTTCC AGAGGGAGAG GTTCCTCCAC CCAGGCCTCC TCCTCCTGAG 
    GAGAAAGACG AGGAGTTCCC AGAGCACAAA GCTGGAGAAA AAGTCAATGA GCCCATAATG 
    GTGGCTGCCA GGCAGCTTCA CGACGAAGCC CGCAAATGGT CTAGCAAAGG AAACGACATA 
    ATTGGTGCTG CCAAACGAAT GGCCCTGCTC ATGGCAGAAA TGTCTCGTTT GGTGCGTGGA 
    GGCAGCGGAA ACAAGCGTGC TCTGATCCAG TGCGCCAAGG ACATCGCCAA AGCCTCCGAC 
    GAAGTCACAC GACTAGCTAA GGAAGTGGCC AAGCAGTGTA CCGACAAACG GATCCGGACC 
    AACCTCCTCC AAGTGTGTGA GCGCATTCCC ACCATCAGCA CTCAGCTCAA AATCCTTTCC 
    ACTGTCAAGG CCACCATGTT GGGCCGCACC AACATCAGTG AAGAAGAGTC GGAGCAGGCG 
    ACTGAAATGT TGGTTCACAA CGCCCAGAAC CTGATGCAAT CAGTAAAGGA GACCGTCAGA 
    GAGGCAGAGG CAGCTTCCAT TAAGATCCGG ACAGATGCAG GTTGCACCCT CCACTGGGTC 
    AGAAAGACCC CCTGGTACCA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    vcl ( XM_015382269.1 ) cDNA ORF clone, Cyprinodon variegatus(sheepshead minnow) -> XP_015237755.1 Cyprinodon variegatus vinculin (vcl), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID OCy01395
    Clone ID Related Accession (Same CDS sequence) XM_015382269.1
    Accession Version XM_015382269.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3201bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-13
    Organism Cyprinodon variegatus(sheepshead minnow)
    Product vinculin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015150465.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cyprinodon variegatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCCGGTTT TCCACACGAA GACCATAGAA AGTATCCTGG AGCCGGTGGC TCAGCAGATA 
    TCCCATCTGG TAATAATGCA CGAGGAAGGT GAAGTTGACG GAAAGGCTAT CCCGGACCTG 
    TCTGCGCCGG TGGCTGCTGT TCAGGCGGCC GTTAGCAACC TCGTTAGGGT TGGAAAGGAT 
    ACTGTGCAAA CAACAGAGGA CCAGATCATG AAAAGGGATA TGCCCCCAGC CTTTATCAAA 
    GTGGAGAATG CCTGTACAAA GCTGGTTCAA GCTGCTTCCA TGCTGAAAGC CGACCCATAC 
    TCTGTTCCGG CTCGGGATTA CCTCATCGAT GGTTCCCGGG GCATTCTGTC TGGGACGTCT 
    GACCTGCTCC TGACTTTTGA TGAGGCAGAG GTCCGCAAAA TAATCCGTGT ATGCAAAGGC 
    ATCTTGGAGT ATCTGAGTGT GGCAGAGGTG GTGGAGTCCA TGGAGGATCT GATCACATAC 
    ACAAAGAATC TGGGACCAGG GATGACCAAG ATGGCGAAGA TGATAGATGA GCGCCAGCAG 
    GAGCTGACAC ATCAGGAACA TCGTGTGATG CTTGTTAACT CGATGAACAC GGTCAAAGAG 
    CTGCTGCCCA TCCTCATATC AGGTATAAAA ATCTTTGTTA CAACCAAGAC ATCTGGGAGC 
    CAGGGTGTCG AGGAGGCCCT GAAGAACCGA AACTTCACGT TTGAGAAGAT GAGTGCTGAG 
    ATAAATGAGA TCATCCGAGT GCTGCAGCTT ACATCCTGGG ATGAAGATGC TTGGGCCAAT 
    AAGGACACAG AGGCCATGAA GAGGGCTCTA GGACTGATCG ACTCCAAAAT GGGTCAGGCT 
    AAAAACTGGC TGAGGGATCC AAATGCTCCA CCAGGGGAGG CTGGCGAGCA GGCCGTCCGG 
    CAGATCCTGG ATGAAGCTGG TAAAGTTGGT GAGCTGTGTG CCGGGAAGGA GCGCAGAGAC 
    ATCCTGGGCA CGGCCAAGAC TCTGGGCCAG ATGACTGATC AGGTGTCTGA GATGAGGGCG 
    AGAGGTCAGG GAGCATCTCC GGCTGCCATG CAGAAAGCGC AGCAGGTTTC CCATGGCCTG 
    GATGTGCTCA CTGGCAAGGT TGAAAACGCC GCTCGCAAAC TCGAGGCCAT GACGAACTCC 
    AAGCAGGCCA TCGCCAAGAG GATCGATGCT GCACAGAGCT GGCTCGCAGA CCCCCACGGT 
    GGCCCAGAGG GAGAGGAGAA TATCAAGGCC CTCCTGGGTG AGGCCAAAAA GATTGCGGAT 
    ATGTGCGAGG ATCCCAAAGA GCGGGAAGAG ATTTTGCGCT CTGCTGGAGA GCTGGCTGCC 
    ATGACGGCCA AACTGTCTGA GCTCAGAAGG CAGGGTAAAG GTGACACTCC TGAGGCCCGA 
    GCCTTAGCCA AGCAGATAGC AACAGCCCTG CAGAACCTGC AGTCCAAGAC CAACAAGGCT 
    GTAGCCAACA GCAGGCCTGC AAAGGCAGCC GTTCACTTGG AGGGGAAGAC GGAGCAGGCG 
    CAGCGCTGGA TCGACAACCC TACTGTGGAC GACAGTGGTG TAGGCCAGGC AGCCATCCGT 
    GGCCTGGTGG CAGAGGGCCG TCGGCTAGCC AATGCTCTAC AGGGTCCGTA CAGGCAGGAG 
    CTGATGGGCA AGTGTGAGCA AGTGGAGCAG CTCATGGCCC AGCTGGCTGA CCTGGCTGCC 
    CGTGGCGAAG GGGACTCCCC GCAAGCACGT GCTGTAGCTC AACAGCTCCA AGAGACCTTG 
    AAAGACCTGA AAGGAAAGAT GCAGGAGGCC ATGACTCAGG AGGTTTCTGA CATCTTCAGT 
    GACACCACCA CCCCTATCAA GTTACTGGCA GTGGCTGCTA CAGCCCCCCT TGATGCACCC 
    AACAGAGATG AGGTCTTTGA TGATAGAGCC AATAACTTCG AGAACCATGC CAACAGGCTT 
    GGTGCTACGG CCGAGAAGGC AGCCGCAGTC GGCACAGCAA ACAAGAACAC GGTGGAGGGA 
    ATCCAGGCTG CCGTCAAGTC AACCAGAGAC CTAACACCAC AAGTGGTCTC TGCTGCTCGT 
    ATTCTGCTGA GAAACCCTGG CAACCAAGCA GCGTATGAAC ATTTTGAGAC CATGAAGAAT 
    CAGTGGATTG ACAACGTGGA AAAAATGACC GGTTTGGTGG ATGAGGCCAT CGATACCAAA 
    TCTCTGCTGG ACGCCTCTGA GGACGCCATA AAGAAGGACT TGGATAAATG CCGCGTGGCC 
    ATGACCAATC ACCAGCCCCA GATGCTGGTG GCCGGTGCTA CGAGCATTGC CCGAAGAGCC 
    AACCGCATCC TTCTGGTGGC GAAACGAGAG GTGGAGAACT CTGAGGACCC AAAATTCAGG 
    GAAATGGTTA AAGCTGCGTC GGATGAACTG AGCCAGACTA TTTCTCCCAT GGTGATGAAT 
    GCAAAAGCTG TGGCCGGAAA CATCCAAGAC CCCAACCTTC AGAAGAGCTT TTTGGACTCG 
    GGTTTTAAGA TTCTTGGAGC TGTGGCAAAG GTCAGGGAAG CCTTCCAGCC CCAAGAGCCA 
    GACTTCCCAC CACCTCCTCC TGAGTTGGAT CAGCTCAATC TCAATGATGA AGCGGCACCT 
    CCAAAGCCTC CTCTTCCAGA GGGAGAGGTT CCTCCACCCA GGCCTCCTCC TCCTGAGGAG 
    AAAGACGAGG AGTTCCCAGA GCACAAAGCT GGAGAAAAAG TCAATGAGCC CATAATGGTG 
    GCTGCCAGGC AGCTTCACGA CGAAGCCCGC AAATGGTCTA GCAAAGGAAA CGACATAATT 
    GGTGCTGCCA AACGAATGGC CCTGCTCATG GCAGAAATGT CTCGTTTGGT GCGTGGAGGC 
    AGCGGAAACA AGCGTGCTCT GATCCAGTGC GCCAAGGACA TCGCCAAAGC CTCCGACGAA 
    GTCACACGAC TAGCTAAGGA AGTGGCCAAG CAGTGTACCG ACAAACGGAT CCGGACCAAC 
    CTCCTCCAAG TGTGTGAGCG CATTCCCACC ATCAGCACTC AGCTCAAAAT CCTTTCCACT 
    GTCAAGGCCA CCATGTTGGG CCGCACCAAC ATCAGTGAAG AAGAGTCGGA GCAGGCGACT 
    GAAATGTTGG TTCACAACGC CCAGAACCTG ATGCAATCAG TAAAGGAGAC CGTCAGAGAG 
    GCAGAGGCAG CTTCCATTAA GATCCGGACA GATGCAGGTT GCACCCTCCA CTGGGTCAGA 
    AAGACCCCCT GGTACCAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015237755.1
    CDS163..3363
    Translation

    Target ORF information:

    RefSeq Version XM_015382269.1
    Organism Cyprinodon variegatus(sheepshead minnow)
    Definition Cyprinodon variegatus vinculin (vcl), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_015382269.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCCGGTTT TCCACACGAA GACCATAGAA AGTATCCTGG AGCCGGTGGC TCAGCAGATA 
    TCCCATCTGG TAATAATGCA CGAGGAAGGT GAAGTTGACG GAAAGGCTAT CCCGGACCTG 
    TCTGCGCCGG TGGCTGCTGT TCAGGCGGCC GTTAGCAACC TCGTTAGGGT TGGAAAGGAT 
    ACTGTGCAAA CAACAGAGGA CCAGATCATG AAAAGGGATA TGCCCCCAGC CTTTATCAAA 
    GTGGAGAATG CCTGTACAAA GCTGGTTCAA GCTGCTTCCA TGCTGAAAGC CGACCCATAC 
    TCTGTTCCGG CTCGGGATTA CCTCATCGAT GGTTCCCGGG GCATTCTGTC TGGGACGTCT 
    GACCTGCTCC TGACTTTTGA TGAGGCAGAG GTCCGCAAAA TAATCCGTGT ATGCAAAGGC 
    ATCTTGGAGT ATCTGAGTGT GGCAGAGGTG GTGGAGTCCA TGGAGGATCT GATCACATAC 
    ACAAAGAATC TGGGACCAGG GATGACCAAG ATGGCGAAGA TGATAGATGA GCGCCAGCAG 
    GAGCTGACAC ATCAGGAACA TCGTGTGATG CTTGTTAACT CGATGAACAC GGTCAAAGAG 
    CTGCTGCCCA TCCTCATATC AGGTATAAAA ATCTTTGTTA CAACCAAGAC ATCTGGGAGC 
    CAGGGTGTCG AGGAGGCCCT GAAGAACCGA AACTTCACGT TTGAGAAGAT GAGTGCTGAG 
    ATAAATGAGA TCATCCGAGT GCTGCAGCTT ACATCCTGGG ATGAAGATGC TTGGGCCAAT 
    AAGGACACAG AGGCCATGAA GAGGGCTCTA GGACTGATCG ACTCCAAAAT GGGTCAGGCT 
    AAAAACTGGC TGAGGGATCC AAATGCTCCA CCAGGGGAGG CTGGCGAGCA GGCCGTCCGG 
    CAGATCCTGG ATGAAGCTGG TAAAGTTGGT GAGCTGTGTG CCGGGAAGGA GCGCAGAGAC 
    ATCCTGGGCA CGGCCAAGAC TCTGGGCCAG ATGACTGATC AGGTGTCTGA GATGAGGGCG 
    AGAGGTCAGG GAGCATCTCC GGCTGCCATG CAGAAAGCGC AGCAGGTTTC CCATGGCCTG 
    GATGTGCTCA CTGGCAAGGT TGAAAACGCC GCTCGCAAAC TCGAGGCCAT GACGAACTCC 
    AAGCAGGCCA TCGCCAAGAG GATCGATGCT GCACAGAGCT GGCTCGCAGA CCCCCACGGT 
    GGCCCAGAGG GAGAGGAGAA TATCAAGGCC CTCCTGGGTG AGGCCAAAAA GATTGCGGAT 
    ATGTGCGAGG ATCCCAAAGA GCGGGAAGAG ATTTTGCGCT CTGCTGGAGA GCTGGCTGCC 
    ATGACGGCCA AACTGTCTGA GCTCAGAAGG CAGGGTAAAG GTGACACTCC TGAGGCCCGA 
    GCCTTAGCCA AGCAGATAGC AACAGCCCTG CAGAACCTGC AGTCCAAGAC CAACAAGGCT 
    GTAGCCAACA GCAGGCCTGC AAAGGCAGCC GTTCACTTGG AGGGGAAGAC GGAGCAGGCG 
    CAGCGCTGGA TCGACAACCC TACTGTGGAC GACAGTGGTG TAGGCCAGGC AGCCATCCGT 
    GGCCTGGTGG CAGAGGGCCG TCGGCTAGCC AATGCTCTAC AGGGTCCGTA CAGGCAGGAG 
    CTGATGGGCA AGTGTGAGCA AGTGGAGCAG CTCATGGCCC AGCTGGCTGA CCTGGCTGCC 
    CGTGGCGAAG GGGACTCCCC GCAAGCACGT GCTGTAGCTC AACAGCTCCA AGAGACCTTG 
    AAAGACCTGA AAGGAAAGAT GCAGGAGGCC ATGACTCAGG AGGTTTCTGA CATCTTCAGT 
    GACACCACCA CCCCTATCAA GTTACTGGCA GTGGCTGCTA CAGCCCCCCT TGATGCACCC 
    AACAGAGATG AGGTCTTTGA TGATAGAGCC AATAACTTCG AGAACCATGC CAACAGGCTT 
    GGTGCTACGG CCGAGAAGGC AGCCGCAGTC GGCACAGCAA ACAAGAACAC GGTGGAGGGA 
    ATCCAGGCTG CCGTCAAGTC AACCAGAGAC CTAACACCAC AAGTGGTCTC TGCTGCTCGT 
    ATTCTGCTGA GAAACCCTGG CAACCAAGCA GCGTATGAAC ATTTTGAGAC CATGAAGAAT 
    CAGTGGATTG ACAACGTGGA AAAAATGACC GGTTTGGTGG ATGAGGCCAT CGATACCAAA 
    TCTCTGCTGG ACGCCTCTGA GGACGCCATA AAGAAGGACT TGGATAAATG CCGCGTGGCC 
    ATGACCAATC ACCAGCCCCA GATGCTGGTG GCCGGTGCTA CGAGCATTGC CCGAAGAGCC 
    AACCGCATCC TTCTGGTGGC GAAACGAGAG GTGGAGAACT CTGAGGACCC AAAATTCAGG 
    GAAATGGTTA AAGCTGCGTC GGATGAACTG AGCCAGACTA TTTCTCCCAT GGTGATGAAT 
    GCAAAAGCTG TGGCCGGAAA CATCCAAGAC CCCAACCTTC AGAAGAGCTT TTTGGACTCG 
    GGTTTTAAGA TTCTTGGAGC TGTGGCAAAG GTCAGGGAAG CCTTCCAGCC CCAAGAGCCA 
    GACTTCCCAC CACCTCCTCC TGAGTTGGAT CAGCTCAATC TCAATGATGA AGCGGCACCT 
    CCAAAGCCTC CTCTTCCAGA GGGAGAGGTT CCTCCACCCA GGCCTCCTCC TCCTGAGGAG 
    AAAGACGAGG AGTTCCCAGA GCACAAAGCT GGAGAAAAAG TCAATGAGCC CATAATGGTG 
    GCTGCCAGGC AGCTTCACGA CGAAGCCCGC AAATGGTCTA GCAAAGGAAA CGACATAATT 
    GGTGCTGCCA AACGAATGGC CCTGCTCATG GCAGAAATGT CTCGTTTGGT GCGTGGAGGC 
    AGCGGAAACA AGCGTGCTCT GATCCAGTGC GCCAAGGACA TCGCCAAAGC CTCCGACGAA 
    GTCACACGAC TAGCTAAGGA AGTGGCCAAG CAGTGTACCG ACAAACGGAT CCGGACCAAC 
    CTCCTCCAAG TGTGTGAGCG CATTCCCACC ATCAGCACTC AGCTCAAAAT CCTTTCCACT 
    GTCAAGGCCA CCATGTTGGG CCGCACCAAC ATCAGTGAAG AAGAGTCGGA GCAGGCGACT 
    GAAATGTTGG TTCACAACGC CCAGAACCTG ATGCAATCAG TAAAGGAGAC CGTCAGAGAG 
    GCAGAGGCAG CTTCCATTAA GATCCGGACA GATGCAGGTT GCACCCTCCA CTGGGTCAGA 
    AAGACCCCCT GGTACCAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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