actn4 cDNA ORF clone, Danio rerio(zebrafish)

The following actn4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the actn4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa04952 NM_199586.1
Latest version!
Danio rerio actinin, alpha 4 (actn4), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
ODa148921 XM_021481335.1
Latest version!
Danio rerio actinin, alpha 4 (actn4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
ODa148921 XM_021481334.1
Latest version!
Danio rerio actinin, alpha 4 (actn4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
ODa148922 XM_021481336.1
Latest version!
Danio rerio actinin, alpha 4 (actn4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
ODa148923 XM_021481337.1
Latest version!
Danio rerio actinin, alpha 4 (actn4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
ODa148924 XM_021481338.1
Latest version!
Danio rerio actinin, alpha 4 (actn4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
ODa148925 XM_021481339.1
Latest version!
Danio rerio actinin, alpha 4 (actn4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa04952
    Clone ID Related Accession (Same CDS sequence) NM_199586.1
    Accession Version NM_199586.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2706bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-26
    Organism Danio rerio(zebrafish)
    Product alpha-actinin-4
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC054901.1. On Jul 6, 2005 this sequence version replaced XM_690966.1. ##Evidence-Data-START## Transcript exon combination :: BC054901.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGTAGATT ATCACGCCGT TAATAATCAA GGCCAGTATT CAGCGGGCGG GCCACCGACC 
    TACATGGAGC AAGAGAACGA CTGGGACCGG GACCTGCTGC TGGACCCGGC CTGGGAGAAG
    CAGCAGAGGA AGACTTTCAC GGCCTGGTGC AACTCTCACC TGAGGAAAGC GGGAACGCAG
    ATCGAAAACA TCGAGGAGGA TTTCAGAGAT GGACTCAAAC TAATGCTGCT CCTGGAGGTC
    ATTTCAGGGG AGAGGTTACC AAAACCTGAA AGAGGCAAGA TGAGAGTGCA CAAGATCAAC
    AATGTCAACA AAGCGCTGGA CTTCATCGCC AGCAAAGGAG TCAAACTGGT GTCCATCGGT
    GCAGAAGAAA TTGTGGATGG AAATGCCAAG ATGACTTTGG GAATGATTTG GACCATCATC
    CTCCGCTTCG CCATCCAGGA CATCTCAGTG GAAGAAACCT CCGCTAAAGA AGGCCTGTTG
    CTCTGGTGTC AGAGAAAGAC GGCTCCTTAC AAGAATGTCA ACGTCCAGAA CTTCCATATC
    AGCTGGAAGG ATGGTCTTGC CTTCAATGCT CTGATCCACA GACACAGACC AGAGCTCATC
    GATTACGACA AGCTGAGAAA GGATGACCCA GTGACCAATC TGAACAATGC TTTCGAAGTG
    GCAGAGCGAT ACCTGGACAT CCCCAAGATG TTGGATGCTG AGGATATCGT GGGCACTCTG
    CGGCCGGATG AGAAGGCTAT TATGACCTAC GTCTCCTGTT TCTATCACGC CTTCTCAGGT
    GCTCAGAAGG CTGAGACGGC AGCCAATCGT ATCTGCAAGG TTTTGGCAGT CAATCAGGAA
    AACGAGCACC TGATGGAGGA CTATGAGAAA CTGGCCAGTG ATCTGTTGGA GTGGATTCGT
    AGGACAATCC CATGGTTGGA AAACCGCGCT CCAGAGAAAA CCATGGCAGA AATGCAGCAG
    AAACTGGAAG ATTTCCGCGA TTACCGTCGT GTTCACAAAC CTCCCAAAGT TCAGGAGAAG
    TGTCAGCTAG AGATCAACTT CAACACGCTG CAGACCAAAC TCCGTTTGAG CAACCGACCG
    GCCTTCATGC CATCTGAAGG ACGCATGGTG TCGGATATTA ATGGTGCGTG GCACAAACTG
    GAAGGGGCAG AAAAAGGCTA TGAAGAGTGG CTCCTGAATG AGATCCGTCG CTTGGAGAGA
    CTTGACCATC TTGCAGAAAA GTTCCGTCAG AAAGCAGCCA TCCATGAGGG CTGGACAGAC
    GGTAAAGAGG CCATGCTCAC ACAAAAGGAC TATGAAACCG CATCCCTGTC TGAGATTAAA
    GCTCTCCTGA AGAAACACGA GGCCTTCGAG AGCGACCTTG CCGCCCACCA GGACAGAGTG
    GAGCAGATTG CCGCCATCGC ACAGGAGCTC AATGAGCTGG ACTACTACGA CTCCCCGAGC
    GTAAATGCAC GCTGTCAGAA GATCTGCGAG CAGTGGGATG CTCTGGGCTC CCTCACACAG
    AACCGCAGAG AGTCTCTGGA GAGAACAGAG AAGCAGCTGG AGTCTATCGA TGAGCTGTAC
    CTGGAGTACG CCAAGAGAGC GGCACCATTC AACAACTGGA TGGAGGGCGC CATGGAGGAC
    CTGCAGGACA TGTTCATTGT GCACAACATC GATGAGATTC AGGGTCTGAT AACAGCTCAT
    GAGCAGTTCA AATCAACTCT TCCAGAGGCA AATAAAGAGC GGGAAGCTAT CCAAGCCATC
    CAGGCTGAAG TTCAGAAAAT TGCAAATTAC AACGGCATCA AACTGGCTGG AAACAATCCT
    TACACCACCA TCACCCCGCA GAGCATTGAC AAGAAGTGGG AGAAGGTGCA GCAACTGGTT
    CCACAGCGTG ATCAGGCACT ACAGGAGGAA CTCACCCGTC AGCAGTCCAA TGATCATCTC
    AGACGCCAGT TTGCCAACCA GGCCAACATG ATCGGACCAT GGATCCAAAA TAAGATGGAG
    GAGATTGGCC GGATATCCAT TGAGATGAAT GGAACTCTAG AGGATCAGCT GACCCACCTC
    AGCCAGTACG AGCAGAGCAT CATCGAATAC AAGCCCAACA TCGACCAACT GGAGGGAGAC
    CATCAGCTCA TTCAGGAAGC ACTTATCTTT GACAACAAAT ACACCGCTTA CACTATGGAG
    CATCTTCGTG TTGGATGGGA GCAGCTCCTC ACTACAATCG CCCGCACCAT TAACGAGATT
    GAGAACCAGG TTCTGACCCG CGATGCTAAA GGCATTAGCC AGGAACAGCT TCACGAGTAC
    CGCACATCCT TCAATCACTT CGACAAGGAT CACAGTGGTC TCCTGCAGTC TGAGGAGTTC
    AAGGCTTGCT TGATCAGTCT GGGTTACGAC GTTGAAAATG ACAAGCAGGG TGATGCTGAA
    TTCGCTCGTA TCATGGGCAT TGTTGACCCC AATAACAGTG GAGCAGTTAC CTTCCAGGCC
    TTCATCGACT TCATGTCAAG GGAAACGACC GACACAGACA CTGCGGACCA GGTCATCGCC
    TCATTTAAGA TCCTGGCTGG AGACAAGAAC TACATCACAG CCGAGGAGCT GAGGCGTGAG
    CTTCCTCCAG ATCAGGCAGA GTACTGCATC GCCCGAATGG CGCCGTACTC TGGGCCGGAT
    GCAGTGGCTG GCGCTCTTGA CTACATGTCC TTCTCCACCG CCCTGTACGG GGAGAGCGAC
    CTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_955880.1
    CDS156..2861
    Translation

    Target ORF information:

    RefSeq Version NM_199586.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio actinin, alpha 4 (actn4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_199586.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGTAGATT ATCACGCCGT TAATAATCAA GGCCAGTATT CAGCGGGCGG GCCACCGACC 
    TACATGGAGC AAGAGAACGA CTGGGACCGG GACCTGCTGC TGGACCCGGC CTGGGAGAAG
    CAGCAGAGGA AGACTTTCAC GGCCTGGTGC AACTCTCACC TGAGGAAAGC GGGAACGCAG
    ATCGAAAACA TCGAGGAGGA TTTCAGAGAT GGACTCAAAC TAATGCTGCT CCTGGAGGTC
    ATTTCAGGGG AGAGGTTACC AAAACCTGAA AGAGGCAAGA TGAGAGTGCA CAAGATCAAC
    AATGTCAACA AAGCGCTGGA CTTCATCGCC AGCAAAGGAG TCAAACTGGT GTCCATCGGT
    GCAGAAGAAA TTGTGGATGG AAATGCCAAG ATGACTTTGG GAATGATTTG GACCATCATC
    CTCCGCTTCG CCATCCAGGA CATCTCAGTG GAAGAAACCT CCGCTAAAGA AGGCCTGTTG
    CTCTGGTGTC AGAGAAAGAC GGCTCCTTAC AAGAATGTCA ACGTCCAGAA CTTCCATATC
    AGCTGGAAGG ATGGTCTTGC CTTCAATGCT CTGATCCACA GACACAGACC AGAGCTCATC
    GATTACGACA AGCTGAGAAA GGATGACCCA GTGACCAATC TGAACAATGC TTTCGAAGTG
    GCAGAGCGAT ACCTGGACAT CCCCAAGATG TTGGATGCTG AGGATATCGT GGGCACTCTG
    CGGCCGGATG AGAAGGCTAT TATGACCTAC GTCTCCTGTT TCTATCACGC CTTCTCAGGT
    GCTCAGAAGG CTGAGACGGC AGCCAATCGT ATCTGCAAGG TTTTGGCAGT CAATCAGGAA
    AACGAGCACC TGATGGAGGA CTATGAGAAA CTGGCCAGTG ATCTGTTGGA GTGGATTCGT
    AGGACAATCC CATGGTTGGA AAACCGCGCT CCAGAGAAAA CCATGGCAGA AATGCAGCAG
    AAACTGGAAG ATTTCCGCGA TTACCGTCGT GTTCACAAAC CTCCCAAAGT TCAGGAGAAG
    TGTCAGCTAG AGATCAACTT CAACACGCTG CAGACCAAAC TCCGTTTGAG CAACCGACCG
    GCCTTCATGC CATCTGAAGG ACGCATGGTG TCGGATATTA ATGGTGCGTG GCACAAACTG
    GAAGGGGCAG AAAAAGGCTA TGAAGAGTGG CTCCTGAATG AGATCCGTCG CTTGGAGAGA
    CTTGACCATC TTGCAGAAAA GTTCCGTCAG AAAGCAGCCA TCCATGAGGG CTGGACAGAC
    GGTAAAGAGG CCATGCTCAC ACAAAAGGAC TATGAAACCG CATCCCTGTC TGAGATTAAA
    GCTCTCCTGA AGAAACACGA GGCCTTCGAG AGCGACCTTG CCGCCCACCA GGACAGAGTG
    GAGCAGATTG CCGCCATCGC ACAGGAGCTC AATGAGCTGG ACTACTACGA CTCCCCGAGC
    GTAAATGCAC GCTGTCAGAA GATCTGCGAG CAGTGGGATG CTCTGGGCTC CCTCACACAG
    AACCGCAGAG AGTCTCTGGA GAGAACAGAG AAGCAGCTGG AGTCTATCGA TGAGCTGTAC
    CTGGAGTACG CCAAGAGAGC GGCACCATTC AACAACTGGA TGGAGGGCGC CATGGAGGAC
    CTGCAGGACA TGTTCATTGT GCACAACATC GATGAGATTC AGGGTCTGAT AACAGCTCAT
    GAGCAGTTCA AATCAACTCT TCCAGAGGCA AATAAAGAGC GGGAAGCTAT CCAAGCCATC
    CAGGCTGAAG TTCAGAAAAT TGCAAATTAC AACGGCATCA AACTGGCTGG AAACAATCCT
    TACACCACCA TCACCCCGCA GAGCATTGAC AAGAAGTGGG AGAAGGTGCA GCAACTGGTT
    CCACAGCGTG ATCAGGCACT ACAGGAGGAA CTCACCCGTC AGCAGTCCAA TGATCATCTC
    AGACGCCAGT TTGCCAACCA GGCCAACATG ATCGGACCAT GGATCCAAAA TAAGATGGAG
    GAGATTGGCC GGATATCCAT TGAGATGAAT GGAACTCTAG AGGATCAGCT GACCCACCTC
    AGCCAGTACG AGCAGAGCAT CATCGAATAC AAGCCCAACA TCGACCAACT GGAGGGAGAC
    CATCAGCTCA TTCAGGAAGC ACTTATCTTT GACAACAAAT ACACCGCTTA CACTATGGAG
    CATCTTCGTG TTGGATGGGA GCAGCTCCTC ACTACAATCG CCCGCACCAT TAACGAGATT
    GAGAACCAGG TTCTGACCCG CGATGCTAAA GGCATTAGCC AGGAACAGCT TCACGAGTAC
    CGCACATCCT TCAATCACTT CGACAAGGAT CACAGTGGTC TCCTGCAGTC TGAGGAGTTC
    AAGGCTTGCT TGATCAGTCT GGGTTACGAC GTTGAAAATG ACAAGCAGGG TGATGCTGAA
    TTCGCTCGTA TCATGGGCAT TGTTGACCCC AATAACAGTG GAGCAGTTAC CTTCCAGGCC
    TTCATCGACT TCATGTCAAG GGAAACGACC GACACAGACA CTGCGGACCA GGTCATCGCC
    TCATTTAAGA TCCTGGCTGG AGACAAGAAC TACATCACAG CCGAGGAGCT GAGGCGTGAG
    CTTCCTCCAG ATCAGGCAGA GTACTGCATC GCCCGAATGG CGCCGTACTC TGGGCCGGAT
    GCAGTGGCTG GCGCTCTTGA CTACATGTCC TTCTCCACCG CCCTGTACGG GGAGAGCGAC
    CTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa148921
    Clone ID Related Accession (Same CDS sequence) XM_021481334.1 , XM_021481335.1
    Accession Version XM_021481334.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2772bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product alpha-actinin-4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGTAGATT ATCACGCCGT TAATAATCAA GGCCAGTATT CAGCGGGCGG GCCACCGACC 
    TACATGGAGC AAGAGAACGA CTGGGACCGG GACCTGCTGC TGGACCCGGC CTGGGAGAAG
    CAGCAGAGGA AGACTTTCAC GGCCTGGTGC AACTCTCACC TGAGGAAAGC GGGAACGCAG
    ATCGAAAACA TCGAGGAGGA TTTCAGAGAT GGACTCAAAC TAATGCTGCT CCTGGAGGTC
    ATTTCAGGGG AGAGGTTACC AAAACCTGAA AGAGGCAAGA TGAGAGTGCA CAAGATCAAC
    AATGTCAACA AAGCGCTGGA CTTCATCGCC AGCAAAGGAG TCAAACTGGT GTCCATCGGT
    GCAGAAGAAA TTGTGGATGG AAATGCCAAG ATGACTTTGG GAATGATTTG GACCATCATC
    CTCCGCTTCG CCATCCAGGA CATCTCAGTG GAAGAAACCT CCGCTAAAGA AGGCCTGTTG
    CTCTGGTGTC AGAGAAAGAC GGCTCCTTAC AAGAATGTCA ACGTCCAGAA CTTCCATATC
    AGCTGGAAGG ATGGTCTTGC CTTCAATGCT CTGATCCACA GACACAGACC AGAGCTCATC
    GATTACGACA AGCTGAGAAA GGATGACCCA GTGACCAATC TGAACAATGC TTTCGAAGTG
    GCAGAGCGAT ACCTGGACAT CCCCAAGATG TTGGATGCTG AGGATATCGT GGGCACTCTG
    CGGCCGGATG AGAAGGCTAT TATGACCTAC GTCTCCTGTT TCTATCACGC CTTCTCAGGT
    GCTCAGAAGG CTGAGACGGC AGCCAATCGT ATCTGCAAGG TTTTGGCAGT CAATCAGGAA
    AACGAGCACC TGATGGAGGA CTATGAGAAA CTGGCCAGTG ATCTGTTGGA GTGGATTCGT
    AGGACAATCC CATGGTTGGA AAACCGCGCT CCAGAGAAAA CCATGGCAGA AATGCAGCAG
    AAACTGGAAG ATTTCCGCGA TTACCGTCGT GTTCACAAAC CTCCCAAAGT TCAGGAGAAG
    TGTCAGCTAG AGATCAACTT CAACACGCTG CAGACCAAAC TCCGTTTGAG CAACCGACCG
    GCCTTCATGC CATCTGAAGG ACGCATGGTG TCGGATATTA ATGGTGCGTG GCACAAACTG
    GAAGGGGCAG AAAAAGGCTA TGAAGAGTGG CTCCTGAATG AGATCCGTCG CTTGGAGAGA
    CTTGACCATC TTGCAGAAAA GTTCCGTCAG AAAGCAGCCA TCCATGAGGG CTGGACAGAC
    GGTAAAGAGG CCATGCTCAC ACAAAAGGAC TATGAAACCG CATCCCTGTC TGAGATTAAA
    GCTCTCCTGA AGAAACACGA GGCCTTCGAG AGCGACCTTG CCGCCCACCA GGACAGAGTG
    GAGCAGATTG CCGCCATCGC ACAGGAGCTC AATGAGCTGG ACTACTACGA CTCCCCGAGC
    GTAAATGCAC GCTGTCAGAA GATCTGCGAG CAGTGGGATG CTCTGGGCTC CCTCACACAG
    AACCGCAGAG AGTCTCTGGA GAGAACAGAG AAGCAGCTGG AGTCTATCGA TGAGCTGTAC
    CTGGAGTACG CCAAGAGAGC GGCACCATTC AACAACTGGA TGGAGGGCGC CATGGAGGAC
    CTGCAGGACA TGTTCATTGT GCACAACATC GATGAGATTC AGGGTCTGAT AACAGCTCAT
    GAGCAGTTCA AATCAACTCT TCCAGAGGCA AATAAAGAGC GGGAAGCTAT CCAAGCCATC
    CAGGCTGAAG TTCAGAAAAT TGCAAATTAC AACGGCATCA AACTGGCTGG AAACAATCCT
    TACACCACCA TCACCCCGCA GAGCATTGAC AAGAAGTGGG AGAAGGTGCA GCAACTGGTT
    CCACAGCGTG ATCAGGCACT ACAGGAGGAA CTCACCCGTC AGCAGTCCAA TGATCATCTC
    AGACGCCAGT TTGCCAACCA GGCCAACATG ATCGGACCAT GGATCCAAAA TAAGATGGAG
    GAGATTGGCC GGATATCCAT TGAGATGAAT GGAACTCTAG AGGATCAGCT GACCCACCTC
    AGCCAGTACG AGCAGAGCAT CATCGAATAC AAGCCCAACA TCGACCAACT GGAGGGAGAC
    CATCAGCTCA TTCAGGAAGC ACTTATCTTT GACAACAAAT ACACCGCTTA CACTATGGAG
    CATCTTCGTG TTGGATGGGA GCAGCTCCTC ACTACAATCG CCCGCACCAT TAACGAGATT
    GAGAACCAGG TTCTGACCCG CGATGCTAAA GGCATTAGCC AGGAACAGCT TCACGAGTAC
    CGCACATCCT TCAATCACTT CGACAAGGAT CACAGTGGTC TCCTGCAGTC TGAGGAGTTC
    AAGGCTTGCT TGATCAGTCT GGGTTACGAC GTTGAAAATG ACAAGCAGAA GCGCACGGGG
    ATAATGGACA GCGATGATTT CCGTGCTCTG CTCATTTCCA CTGGGAACAG CCTGGGTGAT
    GCTGAATTCG CTCGTATCAT GGGCATTGTT GACCCCAATA ACAGTGGAGC AGTTACCTTC
    CAGGCCTTCA TCGACTTCAT GTCAAGGGAA ACGACCGACA CAGACACTGC AGACCAGGTC
    ATCGCCTCAT TTAAGATCCT GGCTGGAGAC AAGAACTACA TCACAGCCGA GGAGCTGAGG
    CGTGAGCTTC CTCCAGATCA GGCAGAGTAC TGCATCGCCC GAATGGCGCC GTACTCTGGG
    CCGGATGCAG TGGCTGGCGC TCTTGACTAC ATGTCCTTCT CCACCGCCCT GTACGGGGAG
    AGCGACCTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021337009.1
    CDS184..2955
    Translation

    Target ORF information:

    RefSeq Version XM_021481334.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio actinin, alpha 4 (actn4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021481334.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGTAGATT ATCACGCCGT TAATAATCAA GGCCAGTATT CAGCGGGCGG GCCACCGACC 
    TACATGGAGC AAGAGAACGA CTGGGACCGG GACCTGCTGC TGGACCCGGC CTGGGAGAAG
    CAGCAGAGGA AGACTTTCAC GGCCTGGTGC AACTCTCACC TGAGGAAAGC GGGAACGCAG
    ATCGAAAACA TCGAGGAGGA TTTCAGAGAT GGACTCAAAC TAATGCTGCT CCTGGAGGTC
    ATTTCAGGGG AGAGGTTACC AAAACCTGAA AGAGGCAAGA TGAGAGTGCA CAAGATCAAC
    AATGTCAACA AAGCGCTGGA CTTCATCGCC AGCAAAGGAG TCAAACTGGT GTCCATCGGT
    GCAGAAGAAA TTGTGGATGG AAATGCCAAG ATGACTTTGG GAATGATTTG GACCATCATC
    CTCCGCTTCG CCATCCAGGA CATCTCAGTG GAAGAAACCT CCGCTAAAGA AGGCCTGTTG
    CTCTGGTGTC AGAGAAAGAC GGCTCCTTAC AAGAATGTCA ACGTCCAGAA CTTCCATATC
    AGCTGGAAGG ATGGTCTTGC CTTCAATGCT CTGATCCACA GACACAGACC AGAGCTCATC
    GATTACGACA AGCTGAGAAA GGATGACCCA GTGACCAATC TGAACAATGC TTTCGAAGTG
    GCAGAGCGAT ACCTGGACAT CCCCAAGATG TTGGATGCTG AGGATATCGT GGGCACTCTG
    CGGCCGGATG AGAAGGCTAT TATGACCTAC GTCTCCTGTT TCTATCACGC CTTCTCAGGT
    GCTCAGAAGG CTGAGACGGC AGCCAATCGT ATCTGCAAGG TTTTGGCAGT CAATCAGGAA
    AACGAGCACC TGATGGAGGA CTATGAGAAA CTGGCCAGTG ATCTGTTGGA GTGGATTCGT
    AGGACAATCC CATGGTTGGA AAACCGCGCT CCAGAGAAAA CCATGGCAGA AATGCAGCAG
    AAACTGGAAG ATTTCCGCGA TTACCGTCGT GTTCACAAAC CTCCCAAAGT TCAGGAGAAG
    TGTCAGCTAG AGATCAACTT CAACACGCTG CAGACCAAAC TCCGTTTGAG CAACCGACCG
    GCCTTCATGC CATCTGAAGG ACGCATGGTG TCGGATATTA ATGGTGCGTG GCACAAACTG
    GAAGGGGCAG AAAAAGGCTA TGAAGAGTGG CTCCTGAATG AGATCCGTCG CTTGGAGAGA
    CTTGACCATC TTGCAGAAAA GTTCCGTCAG AAAGCAGCCA TCCATGAGGG CTGGACAGAC
    GGTAAAGAGG CCATGCTCAC ACAAAAGGAC TATGAAACCG CATCCCTGTC TGAGATTAAA
    GCTCTCCTGA AGAAACACGA GGCCTTCGAG AGCGACCTTG CCGCCCACCA GGACAGAGTG
    GAGCAGATTG CCGCCATCGC ACAGGAGCTC AATGAGCTGG ACTACTACGA CTCCCCGAGC
    GTAAATGCAC GCTGTCAGAA GATCTGCGAG CAGTGGGATG CTCTGGGCTC CCTCACACAG
    AACCGCAGAG AGTCTCTGGA GAGAACAGAG AAGCAGCTGG AGTCTATCGA TGAGCTGTAC
    CTGGAGTACG CCAAGAGAGC GGCACCATTC AACAACTGGA TGGAGGGCGC CATGGAGGAC
    CTGCAGGACA TGTTCATTGT GCACAACATC GATGAGATTC AGGGTCTGAT AACAGCTCAT
    GAGCAGTTCA AATCAACTCT TCCAGAGGCA AATAAAGAGC GGGAAGCTAT CCAAGCCATC
    CAGGCTGAAG TTCAGAAAAT TGCAAATTAC AACGGCATCA AACTGGCTGG AAACAATCCT
    TACACCACCA TCACCCCGCA GAGCATTGAC AAGAAGTGGG AGAAGGTGCA GCAACTGGTT
    CCACAGCGTG ATCAGGCACT ACAGGAGGAA CTCACCCGTC AGCAGTCCAA TGATCATCTC
    AGACGCCAGT TTGCCAACCA GGCCAACATG ATCGGACCAT GGATCCAAAA TAAGATGGAG
    GAGATTGGCC GGATATCCAT TGAGATGAAT GGAACTCTAG AGGATCAGCT GACCCACCTC
    AGCCAGTACG AGCAGAGCAT CATCGAATAC AAGCCCAACA TCGACCAACT GGAGGGAGAC
    CATCAGCTCA TTCAGGAAGC ACTTATCTTT GACAACAAAT ACACCGCTTA CACTATGGAG
    CATCTTCGTG TTGGATGGGA GCAGCTCCTC ACTACAATCG CCCGCACCAT TAACGAGATT
    GAGAACCAGG TTCTGACCCG CGATGCTAAA GGCATTAGCC AGGAACAGCT TCACGAGTAC
    CGCACATCCT TCAATCACTT CGACAAGGAT CACAGTGGTC TCCTGCAGTC TGAGGAGTTC
    AAGGCTTGCT TGATCAGTCT GGGTTACGAC GTTGAAAATG ACAAGCAGAA GCGCACGGGG
    ATAATGGACA GCGATGATTT CCGTGCTCTG CTCATTTCCA CTGGGAACAG CCTGGGTGAT
    GCTGAATTCG CTCGTATCAT GGGCATTGTT GACCCCAATA ACAGTGGAGC AGTTACCTTC
    CAGGCCTTCA TCGACTTCAT GTCAAGGGAA ACGACCGACA CAGACACTGC AGACCAGGTC
    ATCGCCTCAT TTAAGATCCT GGCTGGAGAC AAGAACTACA TCACAGCCGA GGAGCTGAGG
    CGTGAGCTTC CTCCAGATCA GGCAGAGTAC TGCATCGCCC GAATGGCGCC GTACTCTGGG
    CCGGATGCAG TGGCTGGCGC TCTTGACTAC ATGTCCTTCT CCACCGCCCT GTACGGGGAG
    AGCGACCTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa148921
    Clone ID Related Accession (Same CDS sequence) XM_021481334.1 , XM_021481335.1
    Accession Version XM_021481335.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2772bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product alpha-actinin-4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGTAGATT ATCACGCCGT TAATAATCAA GGCCAGTATT CAGCGGGCGG GCCACCGACC 
    TACATGGAGC AAGAGAACGA CTGGGACCGG GACCTGCTGC TGGACCCGGC CTGGGAGAAG
    CAGCAGAGGA AGACTTTCAC GGCCTGGTGC AACTCTCACC TGAGGAAAGC GGGAACGCAG
    ATCGAAAACA TCGAGGAGGA TTTCAGAGAT GGACTCAAAC TAATGCTGCT CCTGGAGGTC
    ATTTCAGGGG AGAGGTTACC AAAACCTGAA AGAGGCAAGA TGAGAGTGCA CAAGATCAAC
    AATGTCAACA AAGCGCTGGA CTTCATCGCC AGCAAAGGAG TCAAACTGGT GTCCATCGGT
    GCAGAAGAAA TTGTGGATGG AAATGCCAAG ATGACTTTGG GAATGATTTG GACCATCATC
    CTCCGCTTCG CCATCCAGGA CATCTCAGTG GAAGAAACCT CCGCTAAAGA AGGCCTGTTG
    CTCTGGTGTC AGAGAAAGAC GGCTCCTTAC AAGAATGTCA ACGTCCAGAA CTTCCATATC
    AGCTGGAAGG ATGGTCTTGC CTTCAATGCT CTGATCCACA GACACAGACC AGAGCTCATC
    GATTACGACA AGCTGAGAAA GGATGACCCA GTGACCAATC TGAACAATGC TTTCGAAGTG
    GCAGAGCGAT ACCTGGACAT CCCCAAGATG TTGGATGCTG AGGATATCGT GGGCACTCTG
    CGGCCGGATG AGAAGGCTAT TATGACCTAC GTCTCCTGTT TCTATCACGC CTTCTCAGGT
    GCTCAGAAGG CTGAGACGGC AGCCAATCGT ATCTGCAAGG TTTTGGCAGT CAATCAGGAA
    AACGAGCACC TGATGGAGGA CTATGAGAAA CTGGCCAGTG ATCTGTTGGA GTGGATTCGT
    AGGACAATCC CATGGTTGGA AAACCGCGCT CCAGAGAAAA CCATGGCAGA AATGCAGCAG
    AAACTGGAAG ATTTCCGCGA TTACCGTCGT GTTCACAAAC CTCCCAAAGT TCAGGAGAAG
    TGTCAGCTAG AGATCAACTT CAACACGCTG CAGACCAAAC TCCGTTTGAG CAACCGACCG
    GCCTTCATGC CATCTGAAGG ACGCATGGTG TCGGATATTA ATGGTGCGTG GCACAAACTG
    GAAGGGGCAG AAAAAGGCTA TGAAGAGTGG CTCCTGAATG AGATCCGTCG CTTGGAGAGA
    CTTGACCATC TTGCAGAAAA GTTCCGTCAG AAAGCAGCCA TCCATGAGGG CTGGACAGAC
    GGTAAAGAGG CCATGCTCAC ACAAAAGGAC TATGAAACCG CATCCCTGTC TGAGATTAAA
    GCTCTCCTGA AGAAACACGA GGCCTTCGAG AGCGACCTTG CCGCCCACCA GGACAGAGTG
    GAGCAGATTG CCGCCATCGC ACAGGAGCTC AATGAGCTGG ACTACTACGA CTCCCCGAGC
    GTAAATGCAC GCTGTCAGAA GATCTGCGAG CAGTGGGATG CTCTGGGCTC CCTCACACAG
    AACCGCAGAG AGTCTCTGGA GAGAACAGAG AAGCAGCTGG AGTCTATCGA TGAGCTGTAC
    CTGGAGTACG CCAAGAGAGC GGCACCATTC AACAACTGGA TGGAGGGCGC CATGGAGGAC
    CTGCAGGACA TGTTCATTGT GCACAACATC GATGAGATTC AGGGTCTGAT AACAGCTCAT
    GAGCAGTTCA AATCAACTCT TCCAGAGGCA AATAAAGAGC GGGAAGCTAT CCAAGCCATC
    CAGGCTGAAG TTCAGAAAAT TGCAAATTAC AACGGCATCA AACTGGCTGG AAACAATCCT
    TACACCACCA TCACCCCGCA GAGCATTGAC AAGAAGTGGG AGAAGGTGCA GCAACTGGTT
    CCACAGCGTG ATCAGGCACT ACAGGAGGAA CTCACCCGTC AGCAGTCCAA TGATCATCTC
    AGACGCCAGT TTGCCAACCA GGCCAACATG ATCGGACCAT GGATCCAAAA TAAGATGGAG
    GAGATTGGCC GGATATCCAT TGAGATGAAT GGAACTCTAG AGGATCAGCT GACCCACCTC
    AGCCAGTACG AGCAGAGCAT CATCGAATAC AAGCCCAACA TCGACCAACT GGAGGGAGAC
    CATCAGCTCA TTCAGGAAGC ACTTATCTTT GACAACAAAT ACACCGCTTA CACTATGGAG
    CATCTTCGTG TTGGATGGGA GCAGCTCCTC ACTACAATCG CCCGCACCAT TAACGAGATT
    GAGAACCAGG TTCTGACCCG CGATGCTAAA GGCATTAGCC AGGAACAGCT TCACGAGTAC
    CGCACATCCT TCAATCACTT CGACAAGGAT CACAGTGGTC TCCTGCAGTC TGAGGAGTTC
    AAGGCTTGCT TGATCAGTCT GGGTTACGAC GTTGAAAATG ACAAGCAGAA GCGCACGGGG
    ATAATGGACA GCGATGATTT CCGTGCTCTG CTCATTTCCA CTGGGAACAG CCTGGGTGAT
    GCTGAATTCG CTCGTATCAT GGGCATTGTT GACCCCAATA ACAGTGGAGC AGTTACCTTC
    CAGGCCTTCA TCGACTTCAT GTCAAGGGAA ACGACCGACA CAGACACTGC AGACCAGGTC
    ATCGCCTCAT TTAAGATCCT GGCTGGAGAC AAGAACTACA TCACAGCCGA GGAGCTGAGG
    CGTGAGCTTC CTCCAGATCA GGCAGAGTAC TGCATCGCCC GAATGGCGCC GTACTCTGGG
    CCGGATGCAG TGGCTGGCGC TCTTGACTAC ATGTCCTTCT CCACCGCCCT GTACGGGGAG
    AGCGACCTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021337010.1
    CDS184..2955
    Translation

    Target ORF information:

    RefSeq Version XM_021481335.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio actinin, alpha 4 (actn4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021481335.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGTAGATT ATCACGCCGT TAATAATCAA GGCCAGTATT CAGCGGGCGG GCCACCGACC 
    TACATGGAGC AAGAGAACGA CTGGGACCGG GACCTGCTGC TGGACCCGGC CTGGGAGAAG
    CAGCAGAGGA AGACTTTCAC GGCCTGGTGC AACTCTCACC TGAGGAAAGC GGGAACGCAG
    ATCGAAAACA TCGAGGAGGA TTTCAGAGAT GGACTCAAAC TAATGCTGCT CCTGGAGGTC
    ATTTCAGGGG AGAGGTTACC AAAACCTGAA AGAGGCAAGA TGAGAGTGCA CAAGATCAAC
    AATGTCAACA AAGCGCTGGA CTTCATCGCC AGCAAAGGAG TCAAACTGGT GTCCATCGGT
    GCAGAAGAAA TTGTGGATGG AAATGCCAAG ATGACTTTGG GAATGATTTG GACCATCATC
    CTCCGCTTCG CCATCCAGGA CATCTCAGTG GAAGAAACCT CCGCTAAAGA AGGCCTGTTG
    CTCTGGTGTC AGAGAAAGAC GGCTCCTTAC AAGAATGTCA ACGTCCAGAA CTTCCATATC
    AGCTGGAAGG ATGGTCTTGC CTTCAATGCT CTGATCCACA GACACAGACC AGAGCTCATC
    GATTACGACA AGCTGAGAAA GGATGACCCA GTGACCAATC TGAACAATGC TTTCGAAGTG
    GCAGAGCGAT ACCTGGACAT CCCCAAGATG TTGGATGCTG AGGATATCGT GGGCACTCTG
    CGGCCGGATG AGAAGGCTAT TATGACCTAC GTCTCCTGTT TCTATCACGC CTTCTCAGGT
    GCTCAGAAGG CTGAGACGGC AGCCAATCGT ATCTGCAAGG TTTTGGCAGT CAATCAGGAA
    AACGAGCACC TGATGGAGGA CTATGAGAAA CTGGCCAGTG ATCTGTTGGA GTGGATTCGT
    AGGACAATCC CATGGTTGGA AAACCGCGCT CCAGAGAAAA CCATGGCAGA AATGCAGCAG
    AAACTGGAAG ATTTCCGCGA TTACCGTCGT GTTCACAAAC CTCCCAAAGT TCAGGAGAAG
    TGTCAGCTAG AGATCAACTT CAACACGCTG CAGACCAAAC TCCGTTTGAG CAACCGACCG
    GCCTTCATGC CATCTGAAGG ACGCATGGTG TCGGATATTA ATGGTGCGTG GCACAAACTG
    GAAGGGGCAG AAAAAGGCTA TGAAGAGTGG CTCCTGAATG AGATCCGTCG CTTGGAGAGA
    CTTGACCATC TTGCAGAAAA GTTCCGTCAG AAAGCAGCCA TCCATGAGGG CTGGACAGAC
    GGTAAAGAGG CCATGCTCAC ACAAAAGGAC TATGAAACCG CATCCCTGTC TGAGATTAAA
    GCTCTCCTGA AGAAACACGA GGCCTTCGAG AGCGACCTTG CCGCCCACCA GGACAGAGTG
    GAGCAGATTG CCGCCATCGC ACAGGAGCTC AATGAGCTGG ACTACTACGA CTCCCCGAGC
    GTAAATGCAC GCTGTCAGAA GATCTGCGAG CAGTGGGATG CTCTGGGCTC CCTCACACAG
    AACCGCAGAG AGTCTCTGGA GAGAACAGAG AAGCAGCTGG AGTCTATCGA TGAGCTGTAC
    CTGGAGTACG CCAAGAGAGC GGCACCATTC AACAACTGGA TGGAGGGCGC CATGGAGGAC
    CTGCAGGACA TGTTCATTGT GCACAACATC GATGAGATTC AGGGTCTGAT AACAGCTCAT
    GAGCAGTTCA AATCAACTCT TCCAGAGGCA AATAAAGAGC GGGAAGCTAT CCAAGCCATC
    CAGGCTGAAG TTCAGAAAAT TGCAAATTAC AACGGCATCA AACTGGCTGG AAACAATCCT
    TACACCACCA TCACCCCGCA GAGCATTGAC AAGAAGTGGG AGAAGGTGCA GCAACTGGTT
    CCACAGCGTG ATCAGGCACT ACAGGAGGAA CTCACCCGTC AGCAGTCCAA TGATCATCTC
    AGACGCCAGT TTGCCAACCA GGCCAACATG ATCGGACCAT GGATCCAAAA TAAGATGGAG
    GAGATTGGCC GGATATCCAT TGAGATGAAT GGAACTCTAG AGGATCAGCT GACCCACCTC
    AGCCAGTACG AGCAGAGCAT CATCGAATAC AAGCCCAACA TCGACCAACT GGAGGGAGAC
    CATCAGCTCA TTCAGGAAGC ACTTATCTTT GACAACAAAT ACACCGCTTA CACTATGGAG
    CATCTTCGTG TTGGATGGGA GCAGCTCCTC ACTACAATCG CCCGCACCAT TAACGAGATT
    GAGAACCAGG TTCTGACCCG CGATGCTAAA GGCATTAGCC AGGAACAGCT TCACGAGTAC
    CGCACATCCT TCAATCACTT CGACAAGGAT CACAGTGGTC TCCTGCAGTC TGAGGAGTTC
    AAGGCTTGCT TGATCAGTCT GGGTTACGAC GTTGAAAATG ACAAGCAGAA GCGCACGGGG
    ATAATGGACA GCGATGATTT CCGTGCTCTG CTCATTTCCA CTGGGAACAG CCTGGGTGAT
    GCTGAATTCG CTCGTATCAT GGGCATTGTT GACCCCAATA ACAGTGGAGC AGTTACCTTC
    CAGGCCTTCA TCGACTTCAT GTCAAGGGAA ACGACCGACA CAGACACTGC AGACCAGGTC
    ATCGCCTCAT TTAAGATCCT GGCTGGAGAC AAGAACTACA TCACAGCCGA GGAGCTGAGG
    CGTGAGCTTC CTCCAGATCA GGCAGAGTAC TGCATCGCCC GAATGGCGCC GTACTCTGGG
    CCGGATGCAG TGGCTGGCGC TCTTGACTAC ATGTCCTTCT CCACCGCCCT GTACGGGGAG
    AGCGACCTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa148922
    Clone ID Related Accession (Same CDS sequence) XM_021481336.1
    Accession Version XM_021481336.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2772bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product alpha-actinin-4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGTAGATT ATCACGCCGT TAATAATCAA GGCCAGTATT CAGCGGGCGG GCCACCGACC 
    TACATGGAGC AAGAGAACGA CTGGGACCGG GACCTGCTGC TGGACCCGGC CTGGGAGAAG
    CAGCAGAGGA AGACTTTCAC GGCCTGGTGC AACTCTCACC TGAGGAAAGC GGGAACGCAG
    ATCGAAAACA TCGAGGAGGA TTTCAGAGAT GGACTCAAAC TAATGCTGCT CCTGGAGGTC
    ATTTCAGGGG AGAGGTTACC AAAACCTGAA AGAGGCAAGA TGAGAGTGCA CAAGATCAAC
    AATGTCAACA AAGCGCTGGA CTTCATCGCC AGCAAAGGAG TCAAACTGGT GTCCATCGGT
    GCAGAAGAAA TTGTGGATGG AAATGCCAAG ATGACTTTGG GAATGATTTG GACCATCATC
    CTCCGCTTCG CCATCCAGGA CATCTCAGTG GAAGAAACCT CCGCTAAAGA AGGCCTGTTG
    CTCTGGTGTC AGAGAAAGAC GGCTCCTTAC AAGAATGTCA ACGTCCAGAA CTTCCATATC
    AGCTGGAAGG ATGGTCTTGC CTTCAATGCT CTGATCCACA GACACAGACC AGAGCTCATC
    GATTACGACA AGCTGAGAAA GGATGACCCA GTGACCAATC TGAACAATGC TTTCGAAGTG
    GCAGAGCGAT ACCTGGACAT CCCCAAGATG TTGGATGCTG AGGACATTGT GAACACAGCA
    CGTCCAGATG AGAAGGCCAT AATGACCTAT GTGTCCAGTT TCTACCATGC CTTTTCTGGA
    GCCCAGAAGG CTGAGACGGC AGCCAATCGT ATCTGCAAGG TTTTGGCAGT CAATCAGGAA
    AACGAGCACC TGATGGAGGA CTATGAGAAA CTGGCCAGTG ATCTGTTGGA GTGGATTCGT
    AGGACAATCC CATGGTTGGA AAACCGCGCT CCAGAGAAAA CCATGGCAGA AATGCAGCAG
    AAACTGGAAG ATTTCCGCGA TTACCGTCGT GTTCACAAAC CTCCCAAAGT TCAGGAGAAG
    TGTCAGCTAG AGATCAACTT CAACACGCTG CAGACCAAAC TCCGTTTGAG CAACCGACCG
    GCCTTCATGC CATCTGAAGG ACGCATGGTG TCGGATATTA ATGGTGCGTG GCACAAACTG
    GAAGGGGCAG AAAAAGGCTA TGAAGAGTGG CTCCTGAATG AGATCCGTCG CTTGGAGAGA
    CTTGACCATC TTGCAGAAAA GTTCCGTCAG AAAGCAGCCA TCCATGAGGG CTGGACAGAC
    GGTAAAGAGG CCATGCTCAC ACAAAAGGAC TATGAAACCG CATCCCTGTC TGAGATTAAA
    GCTCTCCTGA AGAAACACGA GGCCTTCGAG AGCGACCTTG CCGCCCACCA GGACAGAGTG
    GAGCAGATTG CCGCCATCGC ACAGGAGCTC AATGAGCTGG ACTACTACGA CTCCCCGAGC
    GTAAATGCAC GCTGTCAGAA GATCTGCGAG CAGTGGGATG CTCTGGGCTC CCTCACACAG
    AACCGCAGAG AGTCTCTGGA GAGAACAGAG AAGCAGCTGG AGTCTATCGA TGAGCTGTAC
    CTGGAGTACG CCAAGAGAGC GGCACCATTC AACAACTGGA TGGAGGGCGC CATGGAGGAC
    CTGCAGGACA TGTTCATTGT GCACAACATC GATGAGATTC AGGGTCTGAT AACAGCTCAT
    GAGCAGTTCA AATCAACTCT TCCAGAGGCA AATAAAGAGC GGGAAGCTAT CCAAGCCATC
    CAGGCTGAAG TTCAGAAAAT TGCAAATTAC AACGGCATCA AACTGGCTGG AAACAATCCT
    TACACCACCA TCACCCCGCA GAGCATTGAC AAGAAGTGGG AGAAGGTGCA GCAACTGGTT
    CCACAGCGTG ATCAGGCACT ACAGGAGGAA CTCACCCGTC AGCAGTCCAA TGATCATCTC
    AGACGCCAGT TTGCCAACCA GGCCAACATG ATCGGACCAT GGATCCAAAA TAAGATGGAG
    GAGATTGGCC GGATATCCAT TGAGATGAAT GGAACTCTAG AGGATCAGCT GACCCACCTC
    AGCCAGTACG AGCAGAGCAT CATCGAATAC AAGCCCAACA TCGACCAACT GGAGGGAGAC
    CATCAGCTCA TTCAGGAAGC ACTTATCTTT GACAACAAAT ACACCGCTTA CACTATGGAG
    CATCTTCGTG TTGGATGGGA GCAGCTCCTC ACTACAATCG CCCGCACCAT TAACGAGATT
    GAGAACCAGG TTCTGACCCG CGATGCTAAA GGCATTAGCC AGGAACAGCT TCACGAGTAC
    CGCACATCCT TCAATCACTT CGACAAGGAT CACAGTGGTC TCCTGCAGTC TGAGGAGTTC
    AAGGCTTGCT TGATCAGTCT GGGTTACGAC GTTGAAAATG ACAAGCAGAA GCGCACGGGG
    ATAATGGACA GCGATGATTT CCGTGCTCTG CTCATTTCCA CTGGGAACAG CCTGGGTGAT
    GCTGAATTCG CTCGTATCAT GGGCATTGTT GACCCCAATA ACAGTGGAGC AGTTACCTTC
    CAGGCCTTCA TCGACTTCAT GTCAAGGGAA ACGACCGACA CAGACACTGC AGACCAGGTC
    ATCGCCTCAT TTAAGATCCT GGCTGGAGAC AAGAACTACA TCACAGCCGA GGAGCTGAGG
    CGTGAGCTTC CTCCAGATCA GGCAGAGTAC TGCATCGCCC GAATGGCGCC GTACTCTGGG
    CCGGATGCAG TGGCTGGCGC TCTTGACTAC ATGTCCTTCT CCACCGCCCT GTACGGGGAG
    AGCGACCTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021337011.1
    CDS184..2955
    Translation

    Target ORF information:

    RefSeq Version XM_021481336.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio actinin, alpha 4 (actn4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021481336.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGTAGATT ATCACGCCGT TAATAATCAA GGCCAGTATT CAGCGGGCGG GCCACCGACC 
    TACATGGAGC AAGAGAACGA CTGGGACCGG GACCTGCTGC TGGACCCGGC CTGGGAGAAG
    CAGCAGAGGA AGACTTTCAC GGCCTGGTGC AACTCTCACC TGAGGAAAGC GGGAACGCAG
    ATCGAAAACA TCGAGGAGGA TTTCAGAGAT GGACTCAAAC TAATGCTGCT CCTGGAGGTC
    ATTTCAGGGG AGAGGTTACC AAAACCTGAA AGAGGCAAGA TGAGAGTGCA CAAGATCAAC
    AATGTCAACA AAGCGCTGGA CTTCATCGCC AGCAAAGGAG TCAAACTGGT GTCCATCGGT
    GCAGAAGAAA TTGTGGATGG AAATGCCAAG ATGACTTTGG GAATGATTTG GACCATCATC
    CTCCGCTTCG CCATCCAGGA CATCTCAGTG GAAGAAACCT CCGCTAAAGA AGGCCTGTTG
    CTCTGGTGTC AGAGAAAGAC GGCTCCTTAC AAGAATGTCA ACGTCCAGAA CTTCCATATC
    AGCTGGAAGG ATGGTCTTGC CTTCAATGCT CTGATCCACA GACACAGACC AGAGCTCATC
    GATTACGACA AGCTGAGAAA GGATGACCCA GTGACCAATC TGAACAATGC TTTCGAAGTG
    GCAGAGCGAT ACCTGGACAT CCCCAAGATG TTGGATGCTG AGGACATTGT GAACACAGCA
    CGTCCAGATG AGAAGGCCAT AATGACCTAT GTGTCCAGTT TCTACCATGC CTTTTCTGGA
    GCCCAGAAGG CTGAGACGGC AGCCAATCGT ATCTGCAAGG TTTTGGCAGT CAATCAGGAA
    AACGAGCACC TGATGGAGGA CTATGAGAAA CTGGCCAGTG ATCTGTTGGA GTGGATTCGT
    AGGACAATCC CATGGTTGGA AAACCGCGCT CCAGAGAAAA CCATGGCAGA AATGCAGCAG
    AAACTGGAAG ATTTCCGCGA TTACCGTCGT GTTCACAAAC CTCCCAAAGT TCAGGAGAAG
    TGTCAGCTAG AGATCAACTT CAACACGCTG CAGACCAAAC TCCGTTTGAG CAACCGACCG
    GCCTTCATGC CATCTGAAGG ACGCATGGTG TCGGATATTA ATGGTGCGTG GCACAAACTG
    GAAGGGGCAG AAAAAGGCTA TGAAGAGTGG CTCCTGAATG AGATCCGTCG CTTGGAGAGA
    CTTGACCATC TTGCAGAAAA GTTCCGTCAG AAAGCAGCCA TCCATGAGGG CTGGACAGAC
    GGTAAAGAGG CCATGCTCAC ACAAAAGGAC TATGAAACCG CATCCCTGTC TGAGATTAAA
    GCTCTCCTGA AGAAACACGA GGCCTTCGAG AGCGACCTTG CCGCCCACCA GGACAGAGTG
    GAGCAGATTG CCGCCATCGC ACAGGAGCTC AATGAGCTGG ACTACTACGA CTCCCCGAGC
    GTAAATGCAC GCTGTCAGAA GATCTGCGAG CAGTGGGATG CTCTGGGCTC CCTCACACAG
    AACCGCAGAG AGTCTCTGGA GAGAACAGAG AAGCAGCTGG AGTCTATCGA TGAGCTGTAC
    CTGGAGTACG CCAAGAGAGC GGCACCATTC AACAACTGGA TGGAGGGCGC CATGGAGGAC
    CTGCAGGACA TGTTCATTGT GCACAACATC GATGAGATTC AGGGTCTGAT AACAGCTCAT
    GAGCAGTTCA AATCAACTCT TCCAGAGGCA AATAAAGAGC GGGAAGCTAT CCAAGCCATC
    CAGGCTGAAG TTCAGAAAAT TGCAAATTAC AACGGCATCA AACTGGCTGG AAACAATCCT
    TACACCACCA TCACCCCGCA GAGCATTGAC AAGAAGTGGG AGAAGGTGCA GCAACTGGTT
    CCACAGCGTG ATCAGGCACT ACAGGAGGAA CTCACCCGTC AGCAGTCCAA TGATCATCTC
    AGACGCCAGT TTGCCAACCA GGCCAACATG ATCGGACCAT GGATCCAAAA TAAGATGGAG
    GAGATTGGCC GGATATCCAT TGAGATGAAT GGAACTCTAG AGGATCAGCT GACCCACCTC
    AGCCAGTACG AGCAGAGCAT CATCGAATAC AAGCCCAACA TCGACCAACT GGAGGGAGAC
    CATCAGCTCA TTCAGGAAGC ACTTATCTTT GACAACAAAT ACACCGCTTA CACTATGGAG
    CATCTTCGTG TTGGATGGGA GCAGCTCCTC ACTACAATCG CCCGCACCAT TAACGAGATT
    GAGAACCAGG TTCTGACCCG CGATGCTAAA GGCATTAGCC AGGAACAGCT TCACGAGTAC
    CGCACATCCT TCAATCACTT CGACAAGGAT CACAGTGGTC TCCTGCAGTC TGAGGAGTTC
    AAGGCTTGCT TGATCAGTCT GGGTTACGAC GTTGAAAATG ACAAGCAGAA GCGCACGGGG
    ATAATGGACA GCGATGATTT CCGTGCTCTG CTCATTTCCA CTGGGAACAG CCTGGGTGAT
    GCTGAATTCG CTCGTATCAT GGGCATTGTT GACCCCAATA ACAGTGGAGC AGTTACCTTC
    CAGGCCTTCA TCGACTTCAT GTCAAGGGAA ACGACCGACA CAGACACTGC AGACCAGGTC
    ATCGCCTCAT TTAAGATCCT GGCTGGAGAC AAGAACTACA TCACAGCCGA GGAGCTGAGG
    CGTGAGCTTC CTCCAGATCA GGCAGAGTAC TGCATCGCCC GAATGGCGCC GTACTCTGGG
    CCGGATGCAG TGGCTGGCGC TCTTGACTAC ATGTCCTTCT CCACCGCCCT GTACGGGGAG
    AGCGACCTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa148923
    Clone ID Related Accession (Same CDS sequence) XM_021481337.1
    Accession Version XM_021481337.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2706bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product alpha-actinin-4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGTAGATT ATCACGCCGT TAATAATCAA GGCCAGTATT CAGCGGGCGG GCCACCGACC 
    TACATGGAGC AAGAGAACGA CTGGGACCGG GACCTGCTGC TGGACCCGGC CTGGGAGAAG
    CAGCAGAGGA AGACTTTCAC GGCCTGGTGC AACTCTCACC TGAGGAAAGC GGGAACGCAG
    ATCGAAAACA TCGAGGAGGA TTTCAGAGAT GGACTCAAAC TAATGCTGCT CCTGGAGGTC
    ATTTCAGGGG AGAGGTTACC AAAACCTGAA AGAGGCAAGA TGAGAGTGCA CAAGATCAAC
    AATGTCAACA AAGCGCTGGA CTTCATCGCC AGCAAAGGAG TCAAACTGGT GTCCATCGGT
    GCAGAAGAAA TTGTGGATGG AAATGCCAAG ATGACTTTGG GAATGATTTG GACCATCATC
    CTCCGCTTCG CCATCCAGGA CATCTCAGTG GAAGAAACCT CCGCTAAAGA AGGCCTGTTG
    CTCTGGTGTC AGAGAAAGAC GGCTCCTTAC AAGAATGTCA ACGTCCAGAA CTTCCATATC
    AGCTGGAAGG ATGGTCTTGC CTTCAATGCT CTGATCCACA GACACAGACC AGAGCTCATC
    GATTACGACA AGCTGAGAAA GGATGACCCA GTGACCAATC TGAACAATGC TTTCGAAGTG
    GCAGAGCGAT ACCTGGACAT CCCCAAGATG TTGGATGCTG AGGACATTGT GAACACAGCA
    CGTCCAGATG AGAAGGCCAT AATGACCTAT GTGTCCAGTT TCTACCATGC CTTTTCTGGA
    GCCCAGAAGG CTGAGACGGC AGCCAATCGT ATCTGCAAGG TTTTGGCAGT CAATCAGGAA
    AACGAGCACC TGATGGAGGA CTATGAGAAA CTGGCCAGTG ATCTGTTGGA GTGGATTCGT
    AGGACAATCC CATGGTTGGA AAACCGCGCT CCAGAGAAAA CCATGGCAGA AATGCAGCAG
    AAACTGGAAG ATTTCCGCGA TTACCGTCGT GTTCACAAAC CTCCCAAAGT TCAGGAGAAG
    TGTCAGCTAG AGATCAACTT CAACACGCTG CAGACCAAAC TCCGTTTGAG CAACCGACCG
    GCCTTCATGC CATCTGAAGG ACGCATGGTG TCGGATATTA ATGGTGCGTG GCACAAACTG
    GAAGGGGCAG AAAAAGGCTA TGAAGAGTGG CTCCTGAATG AGATCCGTCG CTTGGAGAGA
    CTTGACCATC TTGCAGAAAA GTTCCGTCAG AAAGCAGCCA TCCATGAGGG CTGGACAGAC
    GGTAAAGAGG CCATGCTCAC ACAAAAGGAC TATGAAACCG CATCCCTGTC TGAGATTAAA
    GCTCTCCTGA AGAAACACGA GGCCTTCGAG AGCGACCTTG CCGCCCACCA GGACAGAGTG
    GAGCAGATTG CCGCCATCGC ACAGGAGCTC AATGAGCTGG ACTACTACGA CTCCCCGAGC
    GTAAATGCAC GCTGTCAGAA GATCTGCGAG CAGTGGGATG CTCTGGGCTC CCTCACACAG
    AACCGCAGAG AGTCTCTGGA GAGAACAGAG AAGCAGCTGG AGTCTATCGA TGAGCTGTAC
    CTGGAGTACG CCAAGAGAGC GGCACCATTC AACAACTGGA TGGAGGGCGC CATGGAGGAC
    CTGCAGGACA TGTTCATTGT GCACAACATC GATGAGATTC AGGGTCTGAT AACAGCTCAT
    GAGCAGTTCA AATCAACTCT TCCAGAGGCA AATAAAGAGC GGGAAGCTAT CCAAGCCATC
    CAGGCTGAAG TTCAGAAAAT TGCAAATTAC AACGGCATCA AACTGGCTGG AAACAATCCT
    TACACCACCA TCACCCCGCA GAGCATTGAC AAGAAGTGGG AGAAGGTGCA GCAACTGGTT
    CCACAGCGTG ATCAGGCACT ACAGGAGGAA CTCACCCGTC AGCAGTCCAA TGATCATCTC
    AGACGCCAGT TTGCCAACCA GGCCAACATG ATCGGACCAT GGATCCAAAA TAAGATGGAG
    GAGATTGGCC GGATATCCAT TGAGATGAAT GGAACTCTAG AGGATCAGCT GACCCACCTC
    AGCCAGTACG AGCAGAGCAT CATCGAATAC AAGCCCAACA TCGACCAACT GGAGGGAGAC
    CATCAGCTCA TTCAGGAAGC ACTTATCTTT GACAACAAAT ACACCGCTTA CACTATGGAG
    CATCTTCGTG TTGGATGGGA GCAGCTCCTC ACTACAATCG CCCGCACCAT TAACGAGATT
    GAGAACCAGG TTCTGACCCG CGATGCTAAA GGCATTAGCC AGGAACAGCT TCACGAGTAC
    CGCACATCCT TCAATCACTT CGACAAGGAT CACAGTGGTC TCCTGCAGTC TGAGGAGTTC
    AAGGCTTGCT TGATCAGTCT GGGTTACGAC GTTGAAAATG ACAAGCAGGG TGATGCTGAA
    TTCGCTCGTA TCATGGGCAT TGTTGACCCC AATAACAGTG GAGCAGTTAC CTTCCAGGCC
    TTCATCGACT TCATGTCAAG GGAAACGACC GACACAGACA CTGCAGACCA GGTCATCGCC
    TCATTTAAGA TCCTGGCTGG AGACAAGAAC TACATCACAG CCGAGGAGCT GAGGCGTGAG
    CTTCCTCCAG ATCAGGCAGA GTACTGCATC GCCCGAATGG CGCCGTACTC TGGGCCGGAT
    GCAGTGGCTG GCGCTCTTGA CTACATGTCC TTCTCCACCG CCCTGTACGG GGAGAGCGAC
    CTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021337012.1
    CDS184..2889
    Translation

    Target ORF information:

    RefSeq Version XM_021481337.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio actinin, alpha 4 (actn4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021481337.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGTAGATT ATCACGCCGT TAATAATCAA GGCCAGTATT CAGCGGGCGG GCCACCGACC 
    TACATGGAGC AAGAGAACGA CTGGGACCGG GACCTGCTGC TGGACCCGGC CTGGGAGAAG
    CAGCAGAGGA AGACTTTCAC GGCCTGGTGC AACTCTCACC TGAGGAAAGC GGGAACGCAG
    ATCGAAAACA TCGAGGAGGA TTTCAGAGAT GGACTCAAAC TAATGCTGCT CCTGGAGGTC
    ATTTCAGGGG AGAGGTTACC AAAACCTGAA AGAGGCAAGA TGAGAGTGCA CAAGATCAAC
    AATGTCAACA AAGCGCTGGA CTTCATCGCC AGCAAAGGAG TCAAACTGGT GTCCATCGGT
    GCAGAAGAAA TTGTGGATGG AAATGCCAAG ATGACTTTGG GAATGATTTG GACCATCATC
    CTCCGCTTCG CCATCCAGGA CATCTCAGTG GAAGAAACCT CCGCTAAAGA AGGCCTGTTG
    CTCTGGTGTC AGAGAAAGAC GGCTCCTTAC AAGAATGTCA ACGTCCAGAA CTTCCATATC
    AGCTGGAAGG ATGGTCTTGC CTTCAATGCT CTGATCCACA GACACAGACC AGAGCTCATC
    GATTACGACA AGCTGAGAAA GGATGACCCA GTGACCAATC TGAACAATGC TTTCGAAGTG
    GCAGAGCGAT ACCTGGACAT CCCCAAGATG TTGGATGCTG AGGACATTGT GAACACAGCA
    CGTCCAGATG AGAAGGCCAT AATGACCTAT GTGTCCAGTT TCTACCATGC CTTTTCTGGA
    GCCCAGAAGG CTGAGACGGC AGCCAATCGT ATCTGCAAGG TTTTGGCAGT CAATCAGGAA
    AACGAGCACC TGATGGAGGA CTATGAGAAA CTGGCCAGTG ATCTGTTGGA GTGGATTCGT
    AGGACAATCC CATGGTTGGA AAACCGCGCT CCAGAGAAAA CCATGGCAGA AATGCAGCAG
    AAACTGGAAG ATTTCCGCGA TTACCGTCGT GTTCACAAAC CTCCCAAAGT TCAGGAGAAG
    TGTCAGCTAG AGATCAACTT CAACACGCTG CAGACCAAAC TCCGTTTGAG CAACCGACCG
    GCCTTCATGC CATCTGAAGG ACGCATGGTG TCGGATATTA ATGGTGCGTG GCACAAACTG
    GAAGGGGCAG AAAAAGGCTA TGAAGAGTGG CTCCTGAATG AGATCCGTCG CTTGGAGAGA
    CTTGACCATC TTGCAGAAAA GTTCCGTCAG AAAGCAGCCA TCCATGAGGG CTGGACAGAC
    GGTAAAGAGG CCATGCTCAC ACAAAAGGAC TATGAAACCG CATCCCTGTC TGAGATTAAA
    GCTCTCCTGA AGAAACACGA GGCCTTCGAG AGCGACCTTG CCGCCCACCA GGACAGAGTG
    GAGCAGATTG CCGCCATCGC ACAGGAGCTC AATGAGCTGG ACTACTACGA CTCCCCGAGC
    GTAAATGCAC GCTGTCAGAA GATCTGCGAG CAGTGGGATG CTCTGGGCTC CCTCACACAG
    AACCGCAGAG AGTCTCTGGA GAGAACAGAG AAGCAGCTGG AGTCTATCGA TGAGCTGTAC
    CTGGAGTACG CCAAGAGAGC GGCACCATTC AACAACTGGA TGGAGGGCGC CATGGAGGAC
    CTGCAGGACA TGTTCATTGT GCACAACATC GATGAGATTC AGGGTCTGAT AACAGCTCAT
    GAGCAGTTCA AATCAACTCT TCCAGAGGCA AATAAAGAGC GGGAAGCTAT CCAAGCCATC
    CAGGCTGAAG TTCAGAAAAT TGCAAATTAC AACGGCATCA AACTGGCTGG AAACAATCCT
    TACACCACCA TCACCCCGCA GAGCATTGAC AAGAAGTGGG AGAAGGTGCA GCAACTGGTT
    CCACAGCGTG ATCAGGCACT ACAGGAGGAA CTCACCCGTC AGCAGTCCAA TGATCATCTC
    AGACGCCAGT TTGCCAACCA GGCCAACATG ATCGGACCAT GGATCCAAAA TAAGATGGAG
    GAGATTGGCC GGATATCCAT TGAGATGAAT GGAACTCTAG AGGATCAGCT GACCCACCTC
    AGCCAGTACG AGCAGAGCAT CATCGAATAC AAGCCCAACA TCGACCAACT GGAGGGAGAC
    CATCAGCTCA TTCAGGAAGC ACTTATCTTT GACAACAAAT ACACCGCTTA CACTATGGAG
    CATCTTCGTG TTGGATGGGA GCAGCTCCTC ACTACAATCG CCCGCACCAT TAACGAGATT
    GAGAACCAGG TTCTGACCCG CGATGCTAAA GGCATTAGCC AGGAACAGCT TCACGAGTAC
    CGCACATCCT TCAATCACTT CGACAAGGAT CACAGTGGTC TCCTGCAGTC TGAGGAGTTC
    AAGGCTTGCT TGATCAGTCT GGGTTACGAC GTTGAAAATG ACAAGCAGGG TGATGCTGAA
    TTCGCTCGTA TCATGGGCAT TGTTGACCCC AATAACAGTG GAGCAGTTAC CTTCCAGGCC
    TTCATCGACT TCATGTCAAG GGAAACGACC GACACAGACA CTGCAGACCA GGTCATCGCC
    TCATTTAAGA TCCTGGCTGG AGACAAGAAC TACATCACAG CCGAGGAGCT GAGGCGTGAG
    CTTCCTCCAG ATCAGGCAGA GTACTGCATC GCCCGAATGG CGCCGTACTC TGGGCCGGAT
    GCAGTGGCTG GCGCTCTTGA CTACATGTCC TTCTCCACCG CCCTGTACGG GGAGAGCGAC
    CTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa148924
    Clone ID Related Accession (Same CDS sequence) XM_021481338.1
    Accession Version XM_021481338.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2691bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product alpha-actinin-4 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGTAGATT ATCACGCCGT TAATAATCAA GGCCAGTATT CAGCGGGCGG GCCACCGACC 
    TACATGGAGC AAGAGAACGA CTGGGACCGG GACCTGCTGC TGGACCCGGC CTGGGAGAAG
    CAGCAGAGGA AGACTTTCAC GGCCTGGTGC AACTCTCACC TGAGGAAAGC GGGAACGCAG
    ATCGAAAACA TCGAGGAGGA TTTCAGAGAT GGACTCAAAC TAATGCTGCT CCTGGAGGTC
    ATTTCAGGGG AGAGGTTACC AAAACCTGAA AGAGGCAAGA TGAGAGTGCA CAAGATCAAC
    AATGTCAACA AAGCGCTGGA CTTCATCGCC AGCAAAGGAG TCAAACTGGT GTCCATCGGT
    GCAGAAGAAA TTGTGGATGG AAATGCCAAG ATGACTTTGG GAATGATTTG GACCATCATC
    CTCCGCTTCG CCATCCAGGA CATCTCAGTG GAAGAAACCT CCGCTAAAGA AGGCCTGTTG
    CTCTGGTGTC AGAGAAAGAC GGCTCCTTAC AAGAATGTCA ACGTCCAGAA CTTCCATATC
    AGCTGGAAGG ATGGTCTTGC CTTCAATGCT CTGATCCACA GACACAGACC AGAGCTCATC
    GATTACGACA AGCTGAGAAA GGATGACCCA GTGACCAATC TGAACAATGC TTTCGAAGTG
    GCAGAGCGAT ACCTGGACAT CCCCAAGATG TTGGATGCTG AGGATATCGT GGGCACTCTG
    CGGCCGGATG AGAAGGCTAT TATGACCTAC GTCTCCTGTT TCTATCACGC CTTCTCAGGT
    GCTCAGAAGG CTGAGACGGC AGCCAATCGT ATCTGCAAGG TTTTGGCAGT CAATCAGGAA
    AACGAGCACC TGATGGAGGA CTATGAGAAA CTGGCCAGTG ATCTGTTGGA GTGGATTCGT
    AGGACAATCC CATGGTTGGA AAACCGCGCT CCAGAGAAAA CCATGGCAGA AATGCAGCAG
    AAACTGGAAG ATTTCCGCGA TTACCGTCGT GTTCACAAAC CTCCCAAAGT TCAGGAGAAG
    TGTCAGCTAG AGATCAACTT CAACACGCTG CAGACCAAAC TCCGTTTGAG CAACCGACCG
    GCCTTCATGC CATCTGAAGG ACGCATGGTG TCGGATATTA ATGGTGCGTG GCACAAACTG
    GAAGGGGCAG AAAAAGGCTA TGAAGAGTGG CTCCTGAATG AGATCCGTCG CTTGGAGAGA
    CTTGACCATC TTGCAGAAAA GTTCCGTCAG AAAGCAGCCA TCCATGAGGG CTGGACAGAC
    GGTAAAGAGG CCATGCTCAC ACAAAAGGAC TATGAAACCG CATCCCTGTC TGAGATTAAA
    GCTCTCCTGA AGAAACACGA GGCCTTCGAG AGCGACCTTG CCGCCCACCA GGACAGAGTG
    GAGCAGATTG CCGCCATCGC ACAGGAGCTC AATGAGCTGG ACTACTACGA CTCCCCGAGC
    GTAAATGCAC GCTGTCAGAA GATCTGCGAG CAGTGGGATG CTCTGGGCTC CCTCACACAG
    AACCGCAGAG AGTCTCTGGA GAGAACAGAG AAGCAGCTGG AGTCTATCGA TGAGCTGTAC
    CTGGAGTACG CCAAGAGAGC GGCACCATTC AACAACTGGA TGGAGGGCGC CATGGAGGAC
    CTGCAGGACA TGTTCATTGT GCACAACATC GATGAGATTC AGGGTCTGAT AACAGCTCAT
    GAGCAGTTCA AATCAACTCT TCCAGAGGCA AATAAAGAGC GGGAAGCTAT CCAAGCCATC
    CAGGCTGAAG TTCAGAAAAT TGCAAATTAC AACGGCATCA AACTGGCTGG AAACAATCCT
    TACACCACCA TCACCCCGCA GAGCATTGAC AAGAAGTGGG AGAAGGTGCA GCAACTGGTT
    CCACAGCGTG ATCAGGCACT ACAGGAGGAA CTCACCCGTC AGCAGTCCAA TGATCATCTC
    AGACGCCAGT TTGCCAACCA GGCCAACATG ATCGGACCAT GGATCCAAAA TAAGATGGAG
    GAGATTGGCC GGATATCCAT TGAGATGAAT GGAACTCTAG AGGATCAGCT GACCCACCTC
    AGCCAGTACG AGCAGAGCAT CATCGAATAC AAGCCCAACA TCGACCAACT GGAGGGAGAC
    CATCAGCTCA TTCAGGAAGC ACTTATCTTT GACAACAAAT ACACCGCTTA CACTATGGAG
    CATCTTCGTG TTGGATGGGA GCAGCTCCTC ACTACAATCG CCCGCACCAT TAACGAGATT
    GAGAACCAGG TTCTGACCCG CGATGCTAAA GGCATTAGCC AGGAACAGCT TCACGAGTAC
    CGCACATCCT TCAATCACTT CGACAAGAAG CGCACGGGGA TAATGGACAG CGATGATTTC
    CGTGCTCTGC TCATTTCCAC TGGGAACAGC CTGGGTGATG CTGAATTCGC TCGTATCATG
    GGCATTGTTG ACCCCAATAA CAGTGGAGCA GTTACCTTCC AGGCCTTCAT CGACTTCATG
    TCAAGGGAAA CGACCGACAC AGACACTGCA GACCAGGTCA TCGCCTCATT TAAGATCCTG
    GCTGGAGACA AGAACTACAT CACAGCCGAG GAGCTGAGGC GTGAGCTTCC TCCAGATCAG
    GCAGAGTACT GCATCGCCCG AATGGCGCCG TACTCTGGGC CGGATGCAGT GGCTGGCGCT
    CTTGACTACA TGTCCTTCTC CACCGCCCTG TACGGGGAGA GCGACCTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021337013.1
    CDS184..2874
    Translation

    Target ORF information:

    RefSeq Version XM_021481338.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio actinin, alpha 4 (actn4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021481338.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGTAGATT ATCACGCCGT TAATAATCAA GGCCAGTATT CAGCGGGCGG GCCACCGACC 
    TACATGGAGC AAGAGAACGA CTGGGACCGG GACCTGCTGC TGGACCCGGC CTGGGAGAAG
    CAGCAGAGGA AGACTTTCAC GGCCTGGTGC AACTCTCACC TGAGGAAAGC GGGAACGCAG
    ATCGAAAACA TCGAGGAGGA TTTCAGAGAT GGACTCAAAC TAATGCTGCT CCTGGAGGTC
    ATTTCAGGGG AGAGGTTACC AAAACCTGAA AGAGGCAAGA TGAGAGTGCA CAAGATCAAC
    AATGTCAACA AAGCGCTGGA CTTCATCGCC AGCAAAGGAG TCAAACTGGT GTCCATCGGT
    GCAGAAGAAA TTGTGGATGG AAATGCCAAG ATGACTTTGG GAATGATTTG GACCATCATC
    CTCCGCTTCG CCATCCAGGA CATCTCAGTG GAAGAAACCT CCGCTAAAGA AGGCCTGTTG
    CTCTGGTGTC AGAGAAAGAC GGCTCCTTAC AAGAATGTCA ACGTCCAGAA CTTCCATATC
    AGCTGGAAGG ATGGTCTTGC CTTCAATGCT CTGATCCACA GACACAGACC AGAGCTCATC
    GATTACGACA AGCTGAGAAA GGATGACCCA GTGACCAATC TGAACAATGC TTTCGAAGTG
    GCAGAGCGAT ACCTGGACAT CCCCAAGATG TTGGATGCTG AGGATATCGT GGGCACTCTG
    CGGCCGGATG AGAAGGCTAT TATGACCTAC GTCTCCTGTT TCTATCACGC CTTCTCAGGT
    GCTCAGAAGG CTGAGACGGC AGCCAATCGT ATCTGCAAGG TTTTGGCAGT CAATCAGGAA
    AACGAGCACC TGATGGAGGA CTATGAGAAA CTGGCCAGTG ATCTGTTGGA GTGGATTCGT
    AGGACAATCC CATGGTTGGA AAACCGCGCT CCAGAGAAAA CCATGGCAGA AATGCAGCAG
    AAACTGGAAG ATTTCCGCGA TTACCGTCGT GTTCACAAAC CTCCCAAAGT TCAGGAGAAG
    TGTCAGCTAG AGATCAACTT CAACACGCTG CAGACCAAAC TCCGTTTGAG CAACCGACCG
    GCCTTCATGC CATCTGAAGG ACGCATGGTG TCGGATATTA ATGGTGCGTG GCACAAACTG
    GAAGGGGCAG AAAAAGGCTA TGAAGAGTGG CTCCTGAATG AGATCCGTCG CTTGGAGAGA
    CTTGACCATC TTGCAGAAAA GTTCCGTCAG AAAGCAGCCA TCCATGAGGG CTGGACAGAC
    GGTAAAGAGG CCATGCTCAC ACAAAAGGAC TATGAAACCG CATCCCTGTC TGAGATTAAA
    GCTCTCCTGA AGAAACACGA GGCCTTCGAG AGCGACCTTG CCGCCCACCA GGACAGAGTG
    GAGCAGATTG CCGCCATCGC ACAGGAGCTC AATGAGCTGG ACTACTACGA CTCCCCGAGC
    GTAAATGCAC GCTGTCAGAA GATCTGCGAG CAGTGGGATG CTCTGGGCTC CCTCACACAG
    AACCGCAGAG AGTCTCTGGA GAGAACAGAG AAGCAGCTGG AGTCTATCGA TGAGCTGTAC
    CTGGAGTACG CCAAGAGAGC GGCACCATTC AACAACTGGA TGGAGGGCGC CATGGAGGAC
    CTGCAGGACA TGTTCATTGT GCACAACATC GATGAGATTC AGGGTCTGAT AACAGCTCAT
    GAGCAGTTCA AATCAACTCT TCCAGAGGCA AATAAAGAGC GGGAAGCTAT CCAAGCCATC
    CAGGCTGAAG TTCAGAAAAT TGCAAATTAC AACGGCATCA AACTGGCTGG AAACAATCCT
    TACACCACCA TCACCCCGCA GAGCATTGAC AAGAAGTGGG AGAAGGTGCA GCAACTGGTT
    CCACAGCGTG ATCAGGCACT ACAGGAGGAA CTCACCCGTC AGCAGTCCAA TGATCATCTC
    AGACGCCAGT TTGCCAACCA GGCCAACATG ATCGGACCAT GGATCCAAAA TAAGATGGAG
    GAGATTGGCC GGATATCCAT TGAGATGAAT GGAACTCTAG AGGATCAGCT GACCCACCTC
    AGCCAGTACG AGCAGAGCAT CATCGAATAC AAGCCCAACA TCGACCAACT GGAGGGAGAC
    CATCAGCTCA TTCAGGAAGC ACTTATCTTT GACAACAAAT ACACCGCTTA CACTATGGAG
    CATCTTCGTG TTGGATGGGA GCAGCTCCTC ACTACAATCG CCCGCACCAT TAACGAGATT
    GAGAACCAGG TTCTGACCCG CGATGCTAAA GGCATTAGCC AGGAACAGCT TCACGAGTAC
    CGCACATCCT TCAATCACTT CGACAAGAAG CGCACGGGGA TAATGGACAG CGATGATTTC
    CGTGCTCTGC TCATTTCCAC TGGGAACAGC CTGGGTGATG CTGAATTCGC TCGTATCATG
    GGCATTGTTG ACCCCAATAA CAGTGGAGCA GTTACCTTCC AGGCCTTCAT CGACTTCATG
    TCAAGGGAAA CGACCGACAC AGACACTGCA GACCAGGTCA TCGCCTCATT TAAGATCCTG
    GCTGGAGACA AGAACTACAT CACAGCCGAG GAGCTGAGGC GTGAGCTTCC TCCAGATCAG
    GCAGAGTACT GCATCGCCCG AATGGCGCCG TACTCTGGGC CGGATGCAGT GGCTGGCGCT
    CTTGACTACA TGTCCTTCTC CACCGCCCTG TACGGGGAGA GCGACCTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa148925
    Clone ID Related Accession (Same CDS sequence) XM_021481339.1
    Accession Version XM_021481339.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2691bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product alpha-actinin-4 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGTAGATT ATCACGCCGT TAATAATCAA GGCCAGTATT CAGCGGGCGG GCCACCGACC 
    TACATGGAGC AAGAGAACGA CTGGGACCGG GACCTGCTGC TGGACCCGGC CTGGGAGAAG
    CAGCAGAGGA AGACTTTCAC GGCCTGGTGC AACTCTCACC TGAGGAAAGC GGGAACGCAG
    ATCGAAAACA TCGAGGAGGA TTTCAGAGAT GGACTCAAAC TAATGCTGCT CCTGGAGGTC
    ATTTCAGGGG AGAGGTTACC AAAACCTGAA AGAGGCAAGA TGAGAGTGCA CAAGATCAAC
    AATGTCAACA AAGCGCTGGA CTTCATCGCC AGCAAAGGAG TCAAACTGGT GTCCATCGGT
    GCAGAAGAAA TTGTGGATGG AAATGCCAAG ATGACTTTGG GAATGATTTG GACCATCATC
    CTCCGCTTCG CCATCCAGGA CATCTCAGTG GAAGAAACCT CCGCTAAAGA AGGCCTGTTG
    CTCTGGTGTC AGAGAAAGAC GGCTCCTTAC AAGAATGTCA ACGTCCAGAA CTTCCATATC
    AGCTGGAAGG ATGGTCTTGC CTTCAATGCT CTGATCCACA GACACAGACC AGAGCTCATC
    GATTACGACA AGCTGAGAAA GGATGACCCA GTGACCAATC TGAACAATGC TTTCGAAGTG
    GCAGAGCGAT ACCTGGACAT CCCCAAGATG TTGGATGCTG AGGACATTGT GAACACAGCA
    CGTCCAGATG AGAAGGCCAT AATGACCTAT GTGTCCAGTT TCTACCATGC CTTTTCTGGA
    GCCCAGAAGG CTGAGACGGC AGCCAATCGT ATCTGCAAGG TTTTGGCAGT CAATCAGGAA
    AACGAGCACC TGATGGAGGA CTATGAGAAA CTGGCCAGTG ATCTGTTGGA GTGGATTCGT
    AGGACAATCC CATGGTTGGA AAACCGCGCT CCAGAGAAAA CCATGGCAGA AATGCAGCAG
    AAACTGGAAG ATTTCCGCGA TTACCGTCGT GTTCACAAAC CTCCCAAAGT TCAGGAGAAG
    TGTCAGCTAG AGATCAACTT CAACACGCTG CAGACCAAAC TCCGTTTGAG CAACCGACCG
    GCCTTCATGC CATCTGAAGG ACGCATGGTG TCGGATATTA ATGGTGCGTG GCACAAACTG
    GAAGGGGCAG AAAAAGGCTA TGAAGAGTGG CTCCTGAATG AGATCCGTCG CTTGGAGAGA
    CTTGACCATC TTGCAGAAAA GTTCCGTCAG AAAGCAGCCA TCCATGAGGG CTGGACAGAC
    GGTAAAGAGG CCATGCTCAC ACAAAAGGAC TATGAAACCG CATCCCTGTC TGAGATTAAA
    GCTCTCCTGA AGAAACACGA GGCCTTCGAG AGCGACCTTG CCGCCCACCA GGACAGAGTG
    GAGCAGATTG CCGCCATCGC ACAGGAGCTC AATGAGCTGG ACTACTACGA CTCCCCGAGC
    GTAAATGCAC GCTGTCAGAA GATCTGCGAG CAGTGGGATG CTCTGGGCTC CCTCACACAG
    AACCGCAGAG AGTCTCTGGA GAGAACAGAG AAGCAGCTGG AGTCTATCGA TGAGCTGTAC
    CTGGAGTACG CCAAGAGAGC GGCACCATTC AACAACTGGA TGGAGGGCGC CATGGAGGAC
    CTGCAGGACA TGTTCATTGT GCACAACATC GATGAGATTC AGGGTCTGAT AACAGCTCAT
    GAGCAGTTCA AATCAACTCT TCCAGAGGCA AATAAAGAGC GGGAAGCTAT CCAAGCCATC
    CAGGCTGAAG TTCAGAAAAT TGCAAATTAC AACGGCATCA AACTGGCTGG AAACAATCCT
    TACACCACCA TCACCCCGCA GAGCATTGAC AAGAAGTGGG AGAAGGTGCA GCAACTGGTT
    CCACAGCGTG ATCAGGCACT ACAGGAGGAA CTCACCCGTC AGCAGTCCAA TGATCATCTC
    AGACGCCAGT TTGCCAACCA GGCCAACATG ATCGGACCAT GGATCCAAAA TAAGATGGAG
    GAGATTGGCC GGATATCCAT TGAGATGAAT GGAACTCTAG AGGATCAGCT GACCCACCTC
    AGCCAGTACG AGCAGAGCAT CATCGAATAC AAGCCCAACA TCGACCAACT GGAGGGAGAC
    CATCAGCTCA TTCAGGAAGC ACTTATCTTT GACAACAAAT ACACCGCTTA CACTATGGAG
    CATCTTCGTG TTGGATGGGA GCAGCTCCTC ACTACAATCG CCCGCACCAT TAACGAGATT
    GAGAACCAGG TTCTGACCCG CGATGCTAAA GGCATTAGCC AGGAACAGCT TCACGAGTAC
    CGCACATCCT TCAATCACTT CGACAAGAAG CGCACGGGGA TAATGGACAG CGATGATTTC
    CGTGCTCTGC TCATTTCCAC TGGGAACAGC CTGGGTGATG CTGAATTCGC TCGTATCATG
    GGCATTGTTG ACCCCAATAA CAGTGGAGCA GTTACCTTCC AGGCCTTCAT CGACTTCATG
    TCAAGGGAAA CGACCGACAC AGACACTGCA GACCAGGTCA TCGCCTCATT TAAGATCCTG
    GCTGGAGACA AGAACTACAT CACAGCCGAG GAGCTGAGGC GTGAGCTTCC TCCAGATCAG
    GCAGAGTACT GCATCGCCCG AATGGCGCCG TACTCTGGGC CGGATGCAGT GGCTGGCGCT
    CTTGACTACA TGTCCTTCTC CACCGCCCTG TACGGGGAGA GCGACCTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021337014.1
    CDS184..2874
    Translation

    Target ORF information:

    RefSeq Version XM_021481339.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio actinin, alpha 4 (actn4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021481339.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGTAGATT ATCACGCCGT TAATAATCAA GGCCAGTATT CAGCGGGCGG GCCACCGACC 
    TACATGGAGC AAGAGAACGA CTGGGACCGG GACCTGCTGC TGGACCCGGC CTGGGAGAAG
    CAGCAGAGGA AGACTTTCAC GGCCTGGTGC AACTCTCACC TGAGGAAAGC GGGAACGCAG
    ATCGAAAACA TCGAGGAGGA TTTCAGAGAT GGACTCAAAC TAATGCTGCT CCTGGAGGTC
    ATTTCAGGGG AGAGGTTACC AAAACCTGAA AGAGGCAAGA TGAGAGTGCA CAAGATCAAC
    AATGTCAACA AAGCGCTGGA CTTCATCGCC AGCAAAGGAG TCAAACTGGT GTCCATCGGT
    GCAGAAGAAA TTGTGGATGG AAATGCCAAG ATGACTTTGG GAATGATTTG GACCATCATC
    CTCCGCTTCG CCATCCAGGA CATCTCAGTG GAAGAAACCT CCGCTAAAGA AGGCCTGTTG
    CTCTGGTGTC AGAGAAAGAC GGCTCCTTAC AAGAATGTCA ACGTCCAGAA CTTCCATATC
    AGCTGGAAGG ATGGTCTTGC CTTCAATGCT CTGATCCACA GACACAGACC AGAGCTCATC
    GATTACGACA AGCTGAGAAA GGATGACCCA GTGACCAATC TGAACAATGC TTTCGAAGTG
    GCAGAGCGAT ACCTGGACAT CCCCAAGATG TTGGATGCTG AGGACATTGT GAACACAGCA
    CGTCCAGATG AGAAGGCCAT AATGACCTAT GTGTCCAGTT TCTACCATGC CTTTTCTGGA
    GCCCAGAAGG CTGAGACGGC AGCCAATCGT ATCTGCAAGG TTTTGGCAGT CAATCAGGAA
    AACGAGCACC TGATGGAGGA CTATGAGAAA CTGGCCAGTG ATCTGTTGGA GTGGATTCGT
    AGGACAATCC CATGGTTGGA AAACCGCGCT CCAGAGAAAA CCATGGCAGA AATGCAGCAG
    AAACTGGAAG ATTTCCGCGA TTACCGTCGT GTTCACAAAC CTCCCAAAGT TCAGGAGAAG
    TGTCAGCTAG AGATCAACTT CAACACGCTG CAGACCAAAC TCCGTTTGAG CAACCGACCG
    GCCTTCATGC CATCTGAAGG ACGCATGGTG TCGGATATTA ATGGTGCGTG GCACAAACTG
    GAAGGGGCAG AAAAAGGCTA TGAAGAGTGG CTCCTGAATG AGATCCGTCG CTTGGAGAGA
    CTTGACCATC TTGCAGAAAA GTTCCGTCAG AAAGCAGCCA TCCATGAGGG CTGGACAGAC
    GGTAAAGAGG CCATGCTCAC ACAAAAGGAC TATGAAACCG CATCCCTGTC TGAGATTAAA
    GCTCTCCTGA AGAAACACGA GGCCTTCGAG AGCGACCTTG CCGCCCACCA GGACAGAGTG
    GAGCAGATTG CCGCCATCGC ACAGGAGCTC AATGAGCTGG ACTACTACGA CTCCCCGAGC
    GTAAATGCAC GCTGTCAGAA GATCTGCGAG CAGTGGGATG CTCTGGGCTC CCTCACACAG
    AACCGCAGAG AGTCTCTGGA GAGAACAGAG AAGCAGCTGG AGTCTATCGA TGAGCTGTAC
    CTGGAGTACG CCAAGAGAGC GGCACCATTC AACAACTGGA TGGAGGGCGC CATGGAGGAC
    CTGCAGGACA TGTTCATTGT GCACAACATC GATGAGATTC AGGGTCTGAT AACAGCTCAT
    GAGCAGTTCA AATCAACTCT TCCAGAGGCA AATAAAGAGC GGGAAGCTAT CCAAGCCATC
    CAGGCTGAAG TTCAGAAAAT TGCAAATTAC AACGGCATCA AACTGGCTGG AAACAATCCT
    TACACCACCA TCACCCCGCA GAGCATTGAC AAGAAGTGGG AGAAGGTGCA GCAACTGGTT
    CCACAGCGTG ATCAGGCACT ACAGGAGGAA CTCACCCGTC AGCAGTCCAA TGATCATCTC
    AGACGCCAGT TTGCCAACCA GGCCAACATG ATCGGACCAT GGATCCAAAA TAAGATGGAG
    GAGATTGGCC GGATATCCAT TGAGATGAAT GGAACTCTAG AGGATCAGCT GACCCACCTC
    AGCCAGTACG AGCAGAGCAT CATCGAATAC AAGCCCAACA TCGACCAACT GGAGGGAGAC
    CATCAGCTCA TTCAGGAAGC ACTTATCTTT GACAACAAAT ACACCGCTTA CACTATGGAG
    CATCTTCGTG TTGGATGGGA GCAGCTCCTC ACTACAATCG CCCGCACCAT TAACGAGATT
    GAGAACCAGG TTCTGACCCG CGATGCTAAA GGCATTAGCC AGGAACAGCT TCACGAGTAC
    CGCACATCCT TCAATCACTT CGACAAGAAG CGCACGGGGA TAATGGACAG CGATGATTTC
    CGTGCTCTGC TCATTTCCAC TGGGAACAGC CTGGGTGATG CTGAATTCGC TCGTATCATG
    GGCATTGTTG ACCCCAATAA CAGTGGAGCA GTTACCTTCC AGGCCTTCAT CGACTTCATG
    TCAAGGGAAA CGACCGACAC AGACACTGCA GACCAGGTCA TCGCCTCATT TAAGATCCTG
    GCTGGAGACA AGAACTACAT CACAGCCGAG GAGCTGAGGC GTGAGCTTCC TCCAGATCAG
    GCAGAGTACT GCATCGCCCG AATGGCGCCG TACTCTGGGC CGGATGCAGT GGCTGGCGCT
    CTTGACTACA TGTCCTTCTC CACCGCCCTG TACGGGGAGA GCGACCTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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