col1a2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	col1a2
Entrez Gene ID	336471
Full Name	collagen, type I, alpha 2
Synonyms	alpha2(I),hm:zehn2357,wu:fa98d05,wu:fa99g10,wu:fb04c08,wu:fb11d06,zehn2357
General protein information	Preferred Names collagen, type I, alpha 2 Names collagen alpha-2(I) chain
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005159713.3	XP_005159770.1	collagen alpha-2(I) chain isoform X1
XM_005159713.4	XP_005159770.1	collagen alpha-2(I) chain isoform X1
NM_182968.2	NP_892013.2	collagen alpha-2(I) chain

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	COL1A2	XP_001168894.1
Xenopus tropicalis (tropical clawed frog)	col1a2	NP_001072718.1
Rattus norvegicus (Norway rat)	Col1a2	NP_445808.1
Homo sapiens (human)	COL1A2	NP_000080.2
Mus musculus (house mouse)	Col1a2	NP_031769.2
Gallus gallus (chicken)	COL1A2	NP_001073182.2
	COL1A2	XP_001097831.1
Canis lupus familiaris (dog)	COL1A2	NP_001003187.1
Bos taurus (cattle)	COL1A2	NP_776945.1
Danio rerio (zebrafish)	col1a2	NP_892013.2

Gene Ontology

The following col1a2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the col1a2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa00632	NM_182968.2 Latest version!	Danio rerio collagen, type I, alpha 2 (col1a2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
ODa24381	XM_005159713.4 Latest version!	Danio rerio collagen, type I, alpha 2 (col1a2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
View Old Accession Versions >>
ODa24381	XM_005159713.3	Danio rerio collagen, type I, alpha 2 (col1a2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

col1a2 ( NM_182968.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_892013.2 Danio rerio collagen, type I, alpha 2 (col1a2), mRNA.

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CloneID	ODa00632
Clone ID Related Accession (Same CDS sequence)	NM_182968.2
Accession Version	NM_182968.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4059bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-14
Organism	Danio rerio(zebrafish)
Product	collagen alpha-2(I) chain
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC071278.1. On Jun 16, 2004 this sequence version replaced NM_182968.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC071278.1, AJ318213.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGCTCAGCT TTGTGGATAC CCGGATTCTG TTGCTGCTTG CAGTGACTTC GTACCTAGCA 
TCATGTCAAT CGGGCCTCAA GGGACCAAAA GGACCCAGAG GTGAGAGGGG TCCTAAGGGA 
CCTGATGGAA AACCCGGCCG ACCTGGACTT CCTGGCCCTG CTGGCCCTCC TGGCCCCCCT 
GGTCTTGGTG GAAACTTCGC TGCTCAGTAT GATGGCGCTA AAGGACCTGA CCCCGGCCCT 
GGACCTATGG GTTTGATGGG ACCCAGAGGA CCTTCTGGAT CTCCTGGTGC TCCTGGAGCT 
CAAGGACTGC AAGGACATGC TGGTGAGCCT GGAGAGCCCG GACAGGCTGG AGCTATTGGT 
GCTCGTGGCC CTCCTGGACC CCCTGGCAAA AATGGTGAAG ATGGCAACAA TGGCAGACCT 
GGAAAGCCTG GAGACAGAGG AGTCCTTGGA GCTCAGGGTG CCCGTGGTTT CCCAGGAACT 
CCTGGACTTC CTGGCATGAA GGGACACAGA GGTTACAATG GTATTGATGG ACGCAAAGGA 
GAGCCTGGCG CAGCTGGTGC CAAGGGTGAA AATGGTGCCG CTGGATCAAA TGGAACCCCC 
GGACAGAGAG GTGGTCGTGG TCTTCCTGGT GAGAGAGGTC GTGTTGGCCC TGCTGGCCCA 
GCTGGTGCTC GCGGTGCTGA TGGTAACACT GGTCCTGCTG GCCCTGCTGG ACCTTTGGGC 
TCAGCTGGTC CTCCTGGTTT CCCTGGTGCA CCTGGACCCA AGGGAGAACT TGGACCCGCT 
GGTCCCACTG GACCATCTGG AGCTCAGGGA CAAAGAGGAG AGCCAGGCCC GAACGGTGCC 
GTTGGCCCCG TTGGACCCCC TGGTAACCCT GGTGCTAATG GTATCAATGG TGCTAAAGGA 
GCAGCTGGTC TTCCTGGAAT TGCTGGTGCC CCTGGTTTCC CTGGCCCTAG AGGAGGCCCT 
GGCCCACAGG GACCTAGTGG AGCTTCTGGT CCAAGAGGTC TTGGTGGTGA CCCCGGACCT 
GTTGGAGTGA AGGGAGATTC TGGTGTGAAG GGAGAGCCTG GTAGTGCTGG TCCTCAGGGG 
CCTCCTGGTC CTTCTGGTGA GGAGGGCAAG AGAGGGTCAA CTGGAGAGCA GGGACCTACT 
GGACCTTTGG GACTGCGTGG ACCTAGAGGA GCCGCTGGTA CTCGTGGTCT GCCTGGTCTG 
GCTGGCAGGT CAGGCCCAAT GGGCATGCCT GGTCCTCGTG GTGGTGTTGG TGCACCTGGA 
GCCCGTGGAC CTCCTGGAGA TGCTGGTCGT GCTGGTGAGG CTGGTCTTGT TGGAGCCAGA 
GGTCTCCCTG GTAGCCCTGG CAGTTCTGGA CCCCCTGGAA AGGAAGGACC TTCTGGTGCC 
GCTGGTCAAG ATGGTCGCAC CGGACCCCCT GGCCCAACTG GACCCAGAGG CCAGCCTGGT 
AACATTGGAT TCCCTGGTCC CAAAGGACCT TCTGGCGAGG CTGGCAAGCC TGGTGAGAAG 
GGACCTGTTG GCCCTACTGG ACTGAGAGGC AGCCCTGGTC CTGATGGTAA CAATGGTCCT 
GCTGGTCCTG TTGGACTTGC TGGTGCCCCT GGTGAGAAAG GAGAGCAGGG ACCCTCTGGT 
GCTCCTGGTT TCCAGGGTCT TCCTGGCCCC GCTGGACCTG TTGGTGAGGC TGGCAAGCCT 
GGTGATAGAG GTATCCCTGG AGATCAAGGT GTATCAGGAC CTGCTGGTGT GAAGGGAGAG 
CGTGGTAACC CTGGACCTGC TGGTGCTGCT GGCGCTCAGG GACCCATTGG TGCTCGTGGA 
CCTTCTGGTA CTCCTGGCCC TGATGGAAAC AAGGGAGAGC CTGGTGCAGT TGGACCTGCT 
GGTGCCCCTG GACCACAGGG AGCTGCTGGT ATGCCTGGTG AGCGTGGTGC TGCTGGCACC 
CCTGGAGCTA AGGGAGAGAA GGGTGAGGCT GGATACAGAG GACTGGAGGG CAATGCTGGA 
AAAGATGGTG CCCGTGGTGC TCCTGGACCC AGTGGACCCC CTGGACCTGC TGGTGCTAAT 
GGTGACAAGG GTGAGACTGG CTCCTTTGGT CCTCCTGGAC CTGCTGGTCC TCGTGGTGCC 
CCTGGTGAGC GTGGTGAGTC TGGCCCTGCT GGACCTTCCG GTTTTGCTGG ACCCCCTGGT 
GCTGATGGCC AGACTGGACC AAGAGGTGAG AAAGGACCTG CTGGTGGAAA GGGTGACGCT 
GGACCCGCTG GCCCTGCCGG TCCCGCTGGA AATACTGGAC CCCTTGGTCC TTCTGGTCCT 
GTTGGCCCAC CTGGTGCCCG TGGTGACAGT GGTCCCACTG GTCTGACTGG CTTCCCTGGT 
GCTCCTGGTA GAGTTGGACC TCCTGGTCCT GCTGGTATTG TTGGACCTGC TGGTCTCACT 
GGTCCCGCTG GAAAGGATGG CCCACGTGGT CCTCGTGGTG ATGTTGGCCC TGCTGGTCCC 
CCAGGAGAGA ATGGAATGAT TGGACCTCTT GGTCTGGCTG GTGAGAAGGG ACCCCCTGGA 
GAGGCTGGTG CACCTGGAGC ACCTGGACCT GCTGGGCCTC AGGGTCAGCT GGGATCACAG 
GGATTCAATG GTCTTCCTGG CTCCAGAGGT GACCGTGGTC TTCCTGGTAT CCCCGGATCA 
GTTGGTGAAC CTGGAAGAGT TGGCCCTGCT GGTGCCCCTG GTGCCCGTGG ACCCGGTGGC 
AACATTGGCA TGCCTGGTAT GACTGGTCCT CAGGGTGAGG CTGGACGTGA GGGAAGCCCT 
GGCAATGATG GACCTCCTGG CCGTCCTGGT GCTGCTGGAA TTAAGGGTGA CCGTGGTGAG 
CCTGGTTCCC CTGGAACTGC TGGACCTGTT GGTGCTCCTG GACCCAATGG ACCATCAGGC 
GCTGTTGGCA GACCTGGAAA CCGTGGTGAA TCTGGTCCTT CTGGTCCTAC TGGCGCTGTT 
GGACCTGCTG GAGCAAGAGG TGCACCTGGC CCTGCTGGAC CTCGTGGTGA GAAGGGTGTT 
GCTGGAGAAA AAGGAGACAG GGGTATGAAG GGACTTCGTG GACATCCTGG TCTGCAGGGA 
ATGCCCGGAC CTAACGGTCC TTCTGGTGAC AGTGGGCCTG CTGGCATTGC TGGACCTTCC 
GGTCCAAGAG GCCCAGCTGG TCCCAATGGC CCCGCTGGTA AAGATGGTTC AAATGGCATG 
CCTGGTGCAA TTGGACCCCC AGGACACCGT GGTCCCGCTG GACATGTTGG ACCTGCTGGT 
CCTCCTGGAT CTCCTGGTCT TCCCGGACCC CCTGGCCCAT CTGGAGGTGG ATATGACACA 
TCTGGAGGCT ATGATGAGTA CAGAGCTGAC CAGGCCTCTC TTAGAGCCAA GGATTACGAG 
GTGGATGCCA CCATCAAGTC CTTGAACACT CAGATTGAGA ATCTGCTTTC CCCTGAGGGC 
TCAAAGAAGA ACCCCGCTCG TACTTGCCGT GACATCAGGC TCAGCCACCC AGAATGGAGC 
AGCGGTTTCT ACTGGATCGA CCCCAACCAG GGCTGCACAA TGGATGCCAT CAAGGCCTTC 
TGCGACTTCT CTACTGGCCA GACCTGCATC CACCCCCACC CAGAGAGCAT CCCACGCAAG 
AACTGGTACA GAAGCTCCCA GGAGAAGAAA CACACCTGGT TTGGCGAGAC TATCAACAGT 
GGTACTGAGT TTGCTTACAA TGACGAGACC CTGAGCCCAC AATCCATGGC TACTCAGCTG 
GCCTTCATGC GTCTACTGGC CAACCAGGCA GTCCAGAACA TCACCTACCA CTGCAAGAAC 
AGCATCGCCT ACATGGATGC TGAGAATGGA AACCTGAAGA AGGCTGTGTT GCTGCAGGGT 
TCCAATGATG TTGAGCTCAG GGCGGAGGGC AACAGCCGCT TCACTTTCAG CGTCCTAGAG 
GATGGCTGCA GTAGACACAC TGGCCAGTGG AGCAAGACAG TCATTGAATA CAGAACAAAT 
AAACCATCTC GCCTTCCCAT CCTCGACATT GCACCTTTGG ACATTGGTGG CGCAGATCAA 
GAGTTTGGTT TGGACATTGG CCCAGTCTGT TTCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_892013.2
CDS	179..4237
Translation	MLSFVDTRILLLLAVTSYLASCQSGLKGPKGPRGERGPKGPDGKPGRPGLPGPAGPPGPPGLGGNFAAQYDGAKGPDPGPGPMGLMGPRGPSGSPGAPGAQGLQGHAGEPGEPGQAGAIGARGPPGPPGKNGEDGNNGRPGKPGDRGVLGAQGARGFPGTPGLPGMKGHRGYNGIDGRKGEPGAAGAKGENGAAGSNGTPGQRGGRGLPGERGRVGPAGPAGARGADGNTGPAGPAGPLGSAGPPGFPGAPGPKGELGPAGPTGPSGAQGQRGEPGPNGAVGPVGPPGNPGANGINGAKGAAGLPGIAGAPGFPGPRGGPGPQGPSGASGPRGLGGDPGPVGVKGDSGVKGEPGSAGPQGPPGPSGEEGKRGSTGEQGPTGPLGLRGPRGAAGTRGLPGLAGRSGPMGMPGPRGGVGAPGARGPPGDAGRAGEAGLVGARGLPGSPGSSGPPGKEGPSGAAGQDGRTGPPGPTGPRGQPGNIGFPGPKGPSGEAGKPGEKGPVGPTGLRGSPGPDGNNGPAGPVGLAGAPGEKGEQGPSGAPGFQGLPGPAGPVGEAGKPGDRGIPGDQGVSGPAGVKGERGNPGPAGAAGAQGPIGARGPSGTPGPDGNKGEPGAVGPAGAPGPQGAAGMPGERGAAGTPGAKGEKGEAGYRGLEGNAGKDGARGAPGPSGPPGPAGANGDKGETGSFGPPGPAGPRGAPGERGESGPAGPSGFAGPPGADGQTGPRGEKGPAGGKGDAGPAGPAGPAGNTGPLGPSGPVGPPGARGDSGPTGLTGFPGAPGRVGPPGPAGIVGPAGLTGPAGKDGPRGPRGDVGPAGPPGENGMIGPLGLAGEKGPPGEAGAPGAPGPAGPQGQLGSQGFNGLPGSRGDRGLPGIPGSVGEPGRVGPAGAPGARGPGGNIGMPGMTGPQGEAGREGSPGNDGPPGRPGAAGIKGDRGEPGSPGTAGPVGAPGPNGPSGAVGRPGNRGESGPSGPTGAVGPAGARGAPGPAGPRGEKGVAGEKGDRGMKGLRGHPGLQGMPGPNGPSGDSGPAGIAGPSGPRGPAGPNGPAGKDGSNGMPGAIGPPGHRGPAGHVGPAGPPGSPGLPGPPGPSGGGYDTSGGYDEYRADQASLRAKDYEVDATIKSLNTQIENLLSPEGSKKNPARTCRDIRLSHPEWSSGFYWIDPNQGCTMDAIKAFCDFSTGQTCIHPHPESIPRKNWYRSSQEKKHTWFGETINSGTEFAYNDETLSPQSMATQLAFMRLLANQAVQNITYHCKNSIAYMDAENGNLKKAVLLQGSNDVELRAEGNSRFTFSVLEDGCSRHTGQWSKTVIEYRTNKPSRLPILDIAPLDIGGADQEFGLDIGPVCFK

Target ORF information:

RefSeq Version	NM_182968.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio collagen, type I, alpha 2 (col1a2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_182968.2

ORF Insert Sequence:

ATGCTCAGCT TTGTGGATAC CCGGATTCTG TTGCTGCTTG CAGTGACTTC GTACCTAGCA 
TCATGTCAAT CGGGCCTCAA GGGACCAAAA GGACCCAGAG GTGAGAGGGG TCCTAAGGGA 
CCTGATGGAA AACCCGGCCG ACCTGGACTT CCTGGCCCTG CTGGCCCTCC TGGCCCCCCT 
GGTCTTGGTG GAAACTTCGC TGCTCAGTAT GATGGCGCTA AAGGACCTGA CCCCGGCCCT 
GGACCTATGG GTTTGATGGG ACCCAGAGGA CCTTCTGGAT CTCCTGGTGC TCCTGGAGCT 
CAAGGACTGC AAGGACATGC TGGTGAGCCT GGAGAGCCCG GACAGGCTGG AGCTATTGGT 
GCTCGTGGCC CTCCTGGACC CCCTGGCAAA AATGGTGAAG ATGGCAACAA TGGCAGACCT 
GGAAAGCCTG GAGACAGAGG AGTCCTTGGA GCTCAGGGTG CCCGTGGTTT CCCAGGAACT 
CCTGGACTTC CTGGCATGAA GGGACACAGA GGTTACAATG GTATTGATGG ACGCAAAGGA 
GAGCCTGGCG CAGCTGGTGC CAAGGGTGAA AATGGTGCCG CTGGATCAAA TGGAACCCCC 
GGACAGAGAG GTGGTCGTGG TCTTCCTGGT GAGAGAGGTC GTGTTGGCCC TGCTGGCCCA 
GCTGGTGCTC GCGGTGCTGA TGGTAACACT GGTCCTGCTG GCCCTGCTGG ACCTTTGGGC 
TCAGCTGGTC CTCCTGGTTT CCCTGGTGCA CCTGGACCCA AGGGAGAACT TGGACCCGCT 
GGTCCCACTG GACCATCTGG AGCTCAGGGA CAAAGAGGAG AGCCAGGCCC GAACGGTGCC 
GTTGGCCCCG TTGGACCCCC TGGTAACCCT GGTGCTAATG GTATCAATGG TGCTAAAGGA 
GCAGCTGGTC TTCCTGGAAT TGCTGGTGCC CCTGGTTTCC CTGGCCCTAG AGGAGGCCCT 
GGCCCACAGG GACCTAGTGG AGCTTCTGGT CCAAGAGGTC TTGGTGGTGA CCCCGGACCT 
GTTGGAGTGA AGGGAGATTC TGGTGTGAAG GGAGAGCCTG GTAGTGCTGG TCCTCAGGGG 
CCTCCTGGTC CTTCTGGTGA GGAGGGCAAG AGAGGGTCAA CTGGAGAGCA GGGACCTACT 
GGACCTTTGG GACTGCGTGG ACCTAGAGGA GCCGCTGGTA CTCGTGGTCT GCCTGGTCTG 
GCTGGCAGGT CAGGCCCAAT GGGCATGCCT GGTCCTCGTG GTGGTGTTGG TGCACCTGGA 
GCCCGTGGAC CTCCTGGAGA TGCTGGTCGT GCTGGTGAGG CTGGTCTTGT TGGAGCCAGA 
GGTCTCCCTG GTAGCCCTGG CAGTTCTGGA CCCCCTGGAA AGGAAGGACC TTCTGGTGCC 
GCTGGTCAAG ATGGTCGCAC CGGACCCCCT GGCCCAACTG GACCCAGAGG CCAGCCTGGT 
AACATTGGAT TCCCTGGTCC CAAAGGACCT TCTGGCGAGG CTGGCAAGCC TGGTGAGAAG 
GGACCTGTTG GCCCTACTGG ACTGAGAGGC AGCCCTGGTC CTGATGGTAA CAATGGTCCT 
GCTGGTCCTG TTGGACTTGC TGGTGCCCCT GGTGAGAAAG GAGAGCAGGG ACCCTCTGGT 
GCTCCTGGTT TCCAGGGTCT TCCTGGCCCC GCTGGACCTG TTGGTGAGGC TGGCAAGCCT 
GGTGATAGAG GTATCCCTGG AGATCAAGGT GTATCAGGAC CTGCTGGTGT GAAGGGAGAG 
CGTGGTAACC CTGGACCTGC TGGTGCTGCT GGCGCTCAGG GACCCATTGG TGCTCGTGGA 
CCTTCTGGTA CTCCTGGCCC TGATGGAAAC AAGGGAGAGC CTGGTGCAGT TGGACCTGCT 
GGTGCCCCTG GACCACAGGG AGCTGCTGGT ATGCCTGGTG AGCGTGGTGC TGCTGGCACC 
CCTGGAGCTA AGGGAGAGAA GGGTGAGGCT GGATACAGAG GACTGGAGGG CAATGCTGGA 
AAAGATGGTG CCCGTGGTGC TCCTGGACCC AGTGGACCCC CTGGACCTGC TGGTGCTAAT 
GGTGACAAGG GTGAGACTGG CTCCTTTGGT CCTCCTGGAC CTGCTGGTCC TCGTGGTGCC 
CCTGGTGAGC GTGGTGAGTC TGGCCCTGCT GGACCTTCCG GTTTTGCTGG ACCCCCTGGT 
GCTGATGGCC AGACTGGACC AAGAGGTGAG AAAGGACCTG CTGGTGGAAA GGGTGACGCT 
GGACCCGCTG GCCCTGCCGG TCCCGCTGGA AATACTGGAC CCCTTGGTCC TTCTGGTCCT 
GTTGGCCCAC CTGGTGCCCG TGGTGACAGT GGTCCCACTG GTCTGACTGG CTTCCCTGGT 
GCTCCTGGTA GAGTTGGACC TCCTGGTCCT GCTGGTATTG TTGGACCTGC TGGTCTCACT 
GGTCCCGCTG GAAAGGATGG CCCACGTGGT CCTCGTGGTG ATGTTGGCCC TGCTGGTCCC 
CCAGGAGAGA ATGGAATGAT TGGACCTCTT GGTCTGGCTG GTGAGAAGGG ACCCCCTGGA 
GAGGCTGGTG CACCTGGAGC ACCTGGACCT GCTGGGCCTC AGGGTCAGCT GGGATCACAG 
GGATTCAATG GTCTTCCTGG CTCCAGAGGT GACCGTGGTC TTCCTGGTAT CCCCGGATCA 
GTTGGTGAAC CTGGAAGAGT TGGCCCTGCT GGTGCCCCTG GTGCCCGTGG ACCCGGTGGC 
AACATTGGCA TGCCTGGTAT GACTGGTCCT CAGGGTGAGG CTGGACGTGA GGGAAGCCCT 
GGCAATGATG GACCTCCTGG CCGTCCTGGT GCTGCTGGAA TTAAGGGTGA CCGTGGTGAG 
CCTGGTTCCC CTGGAACTGC TGGACCTGTT GGTGCTCCTG GACCCAATGG ACCATCAGGC 
GCTGTTGGCA GACCTGGAAA CCGTGGTGAA TCTGGTCCTT CTGGTCCTAC TGGCGCTGTT 
GGACCTGCTG GAGCAAGAGG TGCACCTGGC CCTGCTGGAC CTCGTGGTGA GAAGGGTGTT 
GCTGGAGAAA AAGGAGACAG GGGTATGAAG GGACTTCGTG GACATCCTGG TCTGCAGGGA 
ATGCCCGGAC CTAACGGTCC TTCTGGTGAC AGTGGGCCTG CTGGCATTGC TGGACCTTCC 
GGTCCAAGAG GCCCAGCTGG TCCCAATGGC CCCGCTGGTA AAGATGGTTC AAATGGCATG 
CCTGGTGCAA TTGGACCCCC AGGACACCGT GGTCCCGCTG GACATGTTGG ACCTGCTGGT 
CCTCCTGGAT CTCCTGGTCT TCCCGGACCC CCTGGCCCAT CTGGAGGTGG ATATGACACA 
TCTGGAGGCT ATGATGAGTA CAGAGCTGAC CAGGCCTCTC TTAGAGCCAA GGATTACGAG 
GTGGATGCCA CCATCAAGTC CTTGAACACT CAGATTGAGA ATCTGCTTTC CCCTGAGGGC 
TCAAAGAAGA ACCCCGCTCG TACTTGCCGT GACATCAGGC TCAGCCACCC AGAATGGAGC 
AGCGGTTTCT ACTGGATCGA CCCCAACCAG GGCTGCACAA TGGATGCCAT CAAGGCCTTC 
TGCGACTTCT CTACTGGCCA GACCTGCATC CACCCCCACC CAGAGAGCAT CCCACGCAAG 
AACTGGTACA GAAGCTCCCA GGAGAAGAAA CACACCTGGT TTGGCGAGAC TATCAACAGT 
GGTACTGAGT TTGCTTACAA TGACGAGACC CTGAGCCCAC AATCCATGGC TACTCAGCTG 
GCCTTCATGC GTCTACTGGC CAACCAGGCA GTCCAGAACA TCACCTACCA CTGCAAGAAC 
AGCATCGCCT ACATGGATGC TGAGAATGGA AACCTGAAGA AGGCTGTGTT GCTGCAGGGT 
TCCAATGATG TTGAGCTCAG GGCGGAGGGC AACAGCCGCT TCACTTTCAG CGTCCTAGAG 
GATGGCTGCA GTAGACACAC TGGCCAGTGG AGCAAGACAG TCATTGAATA CAGAACAAAT 
AAACCATCTC GCCTTCCCAT CCTCGACATT GCACCTTTGG ACATTGGTGG CGCAGATCAA 
GAGTTTGGTT TGGACATTGG CCCAGTCTGT TTCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col1a2 ( XM_005159713.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005159770.1 Danio rerio collagen, type I, alpha 2 (col1a2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa24381
Clone ID Related Accession (Same CDS sequence)	XM_005159713.3 , XM_005159713.4
Accession Version	XM_005159713.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3960bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	collagen alpha-2(I) chain isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005159713.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAGCT TTGTGGATAC CCGGATTCTG TTGCTGCTTG CAGTGACTTC GTACCTAGCA 
TCATGTCAAT CGGGCCTCAA GGGACCAAAA GGACCCAGAG GTGAGAGGGG TCCTAAGGGA 
CCTGATGGAA AACCCGGCCG ACCTGGACTT CCTGGCCCTG CTGGCCCTCC TGGCCCCCCT 
GGTCTTGGTG GAAACTTCGC TGCTCAGTAT GATGGCGCTA AAGGACCTGA CCCCGGCCCT 
GGACCTATGG GTTTGATGGG ACCCAGAGGA CCTTCTGGAT CTCCTGGTGC TCCTGGAGCT 
CAAGGACTGC AAGGACATGC TGGTGAGCCT GGAGAGCCCG GACAGGCTGG AGCTATTGGT 
GCTCGTGGCC CTCCTGGACC CCCTGGCAAA AATGGTGAAG ATGGCAACAA TGGCAGACCT 
GGAAAGCCTG GAGACAGAGG AGTCCTTGGA GCTCAGGGTG CCCGTGGTTT CCCAGGAACT 
CCTGGACTTC CTGGCATGAA GGGACACAGA GGTTACAATG GTATTGATGG ACGCAAAGGA 
GAGCCTGGCG CAGCTGGTGC CAAGGGTGAA AATGGTGCCG CTGGATCAAA TGGAACCCCC 
GGACAGAGAG GTGGTCGTGG TCTTCCTGGT GAGAGAGGTC GTGTTGGCCC TGCTGGCCCA 
GCTGGTGCTC GCGGTGCTGA TGGTAACACT GGTCCTGCTG GCCCTGCTGG ACCTTTGGGC 
TCAGCTGGTC CTCCTGGTTT CCCTGGTGCA CCTGGACCCA AGGGAGAACT TGGACCCGCT 
GGTCCCACTG GACCATCTGG AGCTCAGGGA CAAAGAGGAG AGCCAGGCCC GAACGGTGCC 
GTTGGCCCCG TTGGACCCCC TGGTAACCCT GGTGCTAATG GTATCAATGG TGCTAAAGGA 
GCAGCTGGTC TTCCTGGAAT TGCTGGTGCC CCTGGTTTCC CTGGCCCTAG AGGAGGCCCT 
GGCCCACAGG GACCTAGTGG AGCTTCTGGT CCAAGAGGTC TTGGTGGTGA CCCCGGACCT 
GTTGGAGTGA AGGGAGATTC TGGTGTGAAG GGAGAGCCTG GTAGTGCTGG TCCTCAGGGG 
CCTCCTGGTC CTTCTGGTGA GGAGGGCAAG AGAGGGTCAA CTGGAGAGCA GGGACCTACT 
GGACCTTTGG GACTGCGTGG ACCTAGAGGA GCCGCTGGTA CTCGTGGTCT GCCTGGTCTG 
GCTGGCAGGT CAGGCCCAAT GGGCATGCCT GGTCCTCGTG GTGGTGTTGG TGCACCTGGA 
GCCCGTGGAC CTCCTGGAGA TGCTGGTCGT GCTGGTGAGG CTGGTCTTGT TGGAGCCAGA 
GGTCTCCCTG GTAGCCCTGG CAGTTCTGGA CCCCCTGGAA AGGAAGGACC TTCTGGCGAG 
GCTGGCAAGC CTGGTGAGAA GGGACCTGTT GGCCCTACTG GACTGAGAGG CAGCCCTGGT 
CCTGATGGTA ACAATGGTCC TGCTGGTCCT GTTGGACTTG CTGGTGCCCC TGGTGAGAAA 
GGAGAGCAGG GACCCTCTGG TGCTCCTGGT TTCCAGGGTC TTCCTGGCCC CGCTGGACCT 
GTTGGTGAGG CTGGCAAGCC TGGTGATAGA GGTATCCCTG GAGATCAAGG TGTATCAGGA 
CCTGCTGGTG TGAAGGGAGA GCGTGGTAAC CCTGGACCTG CTGGTGCTGC TGGCGCTCAG 
GGACCCATTG GTGCTCGTGG ACCTTCTGGT ACTCCTGGCC CTGATGGAAA CAAGGGAGAG 
CCTGGTGCAG TTGGACCTGC TGGTGCCCCT GGACCACAGG GAGCTGCTGG TATGCCTGGT 
GAGCGTGGTG CTGCTGGCAC CCCTGGAGCT AAGGGAGAGA AGGGTGAGGC TGGATACAGA 
GGACTGGAGG GCAATGCTGG AAAAGATGGT GCCCGTGGTG CTCCTGGACC CAGTGGACCC 
CCTGGACCTG CTGGTGCTAA TGGTGACAAG GGTGAGACTG GCTCCTTTGG TCCTCCTGGA 
CCTGCTGGTC CTCGTGGTGC CCCTGGTGAG CGTGGTGAGT CTGGCCCTGC TGGACCTTCC 
GGTTTTGCTG GACCCCCTGG TGCTGATGGC CAGACTGGAC CAAGAGGTGA GAAAGGACCT 
GCTGGTGGAA AGGGTGACGC TGGACCCGCT GGCCCTGCCG GTCCCGCTGG AAATACTGGA 
CCCCTTGGTC CTTCTGGTCC TGTTGGCCCA CCTGGTGCCC GTGGTGACAG TGGTCCCACT 
GGTCTGACTG GCTTCCCTGG TGCTCCTGGT AGAGTTGGAC CTCCTGGTCC TGCTGGTATT 
GTTGGACCTG CTGGTCTCAC TGGTCCCGCT GGAAAGGATG GCCCACGTGG TCCTCGTGGT 
GATGTTGGCC CTGCTGGTCC CCCAGGAGAG AATGGAATGA TTGGACCTCT TGGTCTGGCT 
GGTGAGAAGG GACCCCCTGG AGAGGCTGGT GCACCTGGAG CACCTGGACC TGCTGGGCCT 
CAGGGTCAGC TGGGATCACA GGGATTCAAT GGTCTTCCTG GCTCCAGAGG TGACCGTGGT 
CTTCCTGGTA TCCCCGGATC AGTTGGTGAA CCTGGAAGAG TTGGCCCTGC TGGTGCCCCT 
GGTGCCCGTG GACCCGGTGG CAACATTGGC ATGCCTGGTA TGACTGGTCC TCAGGGTGAG 
GCTGGACGTG AGGGAAGCCC TGGCAATGAT GGACCTCCTG GCCGTCCTGG TGCTGCTGGA 
ATTAAGGGTG ACCGTGGTGA GCCTGGTTCC CCTGGAACTG CTGGACCTGT TGGTGCTCCT 
GGACCCAATG GACCATCAGG CGCTGTTGGC AGACCTGGAA ACCGTGGTGA ATCTGGTCCT 
TCTGGTCCTA CTGGCGCTGT TGGACCTGCT GGAGCAAGAG GTGCACCTGG CCCTGCTGGA 
CCTCGTGGTG AGAAGGGTGT TGCTGGAGAA AAAGGAGACA GGGGTATGAA GGGACTTCGT 
GGACATCCTG GTCTGCAGGG AATGCCCGGA CCTAACGGTC CTTCTGGTGA CAGTGGGCCT 
GCTGGCATTG CTGGACCTTC CGGTCCAAGA GGCCCAGCTG GTCCCAATGG CCCCGCTGGT 
AAAGATGGTT CAAATGGCAT GCCTGGTGCA ATTGGACCCC CAGGACACCG TGGTCCCGCT 
GGACATGTTG GACCTGCTGG TCCTCCTGGA TCTCCTGGTC TTCCCGGACC CCCTGGCCCA 
TCTGGAGGTG GATATGACAC ATCTGGAGGC TATGATGAGT ACAGAGCTGA CCAGGCCTCT 
CTTAGAGCCA AGGATTACGA GGTGGATGCC ACCATCAAGT CCTTGAACAC TCAGATTGAG 
AATCTGCTTT CCCCTGAGGG CTCAAAGAAG AACCCCGCTC GTACTTGCCG TGACATCAGG 
CTCAGCCACC CAGAATGGAG CAGCGGTTTC TACTGGATCG ACCCCAACCA GGGCTGCACA 
ATGGATGCCA TCAAGGCCTT CTGCGACTTC TCTACTGGCC AGACCTGCAT CCACCCCCAC 
CCAGAGAGCA TCCCACGCAA GAACTGGTAC AGAAGCTCCC AGGAGAAGAA ACACACCTGG 
TTTGGCGAGA CTATCAACAG TGGTACTGAG TTTGCTTACA ATGACGAGAC CCTGAGCCCA 
CAATCCATGG CTACTCAGCT GGCCTTCATG CGTCTACTGG CCAACCAGGC AGTCCAGAAC 
ATCACCTACC ACTGCAAGAA CAGCATCGCC TACATGGATG CTGAGAATGG AAACCTGAAG 
AAGGCTGTGT TGCTGCAGGG TTCCAATGAT GTTGAGCTCA GGGCGGAGGG CAACAGCCGC 
TTCACTTTCA GCGTCCTAGA GGATGGCTGC AGTAGACACA CTGGCCAGTG GAGCAAGACA 
GTCATTGAAT ACAGAACAAA TAAACCATCT CGCCTTCCCA TCCTCGACAT TGCACCTTTG 
GACATTGGTG GCGCAGATCA AGAGTTTGGT TTGGACATTG GCCCAGTCTG TTTCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159770.1
CDS	188..4147
Translation	MLSFVDTRILLLLAVTSYLASCQSGLKGPKGPRGERGPKGPDGKPGRPGLPGPAGPPGPPGLGGNFAAQYDGAKGPDPGPGPMGLMGPRGPSGSPGAPGAQGLQGHAGEPGEPGQAGAIGARGPPGPPGKNGEDGNNGRPGKPGDRGVLGAQGARGFPGTPGLPGMKGHRGYNGIDGRKGEPGAAGAKGENGAAGSNGTPGQRGGRGLPGERGRVGPAGPAGARGADGNTGPAGPAGPLGSAGPPGFPGAPGPKGELGPAGPTGPSGAQGQRGEPGPNGAVGPVGPPGNPGANGINGAKGAAGLPGIAGAPGFPGPRGGPGPQGPSGASGPRGLGGDPGPVGVKGDSGVKGEPGSAGPQGPPGPSGEEGKRGSTGEQGPTGPLGLRGPRGAAGTRGLPGLAGRSGPMGMPGPRGGVGAPGARGPPGDAGRAGEAGLVGARGLPGSPGSSGPPGKEGPSGEAGKPGEKGPVGPTGLRGSPGPDGNNGPAGPVGLAGAPGEKGEQGPSGAPGFQGLPGPAGPVGEAGKPGDRGIPGDQGVSGPAGVKGERGNPGPAGAAGAQGPIGARGPSGTPGPDGNKGEPGAVGPAGAPGPQGAAGMPGERGAAGTPGAKGEKGEAGYRGLEGNAGKDGARGAPGPSGPPGPAGANGDKGETGSFGPPGPAGPRGAPGERGESGPAGPSGFAGPPGADGQTGPRGEKGPAGGKGDAGPAGPAGPAGNTGPLGPSGPVGPPGARGDSGPTGLTGFPGAPGRVGPPGPAGIVGPAGLTGPAGKDGPRGPRGDVGPAGPPGENGMIGPLGLAGEKGPPGEAGAPGAPGPAGPQGQLGSQGFNGLPGSRGDRGLPGIPGSVGEPGRVGPAGAPGARGPGGNIGMPGMTGPQGEAGREGSPGNDGPPGRPGAAGIKGDRGEPGSPGTAGPVGAPGPNGPSGAVGRPGNRGESGPSGPTGAVGPAGARGAPGPAGPRGEKGVAGEKGDRGMKGLRGHPGLQGMPGPNGPSGDSGPAGIAGPSGPRGPAGPNGPAGKDGSNGMPGAIGPPGHRGPAGHVGPAGPPGSPGLPGPPGPSGGGYDTSGGYDEYRADQASLRAKDYEVDATIKSLNTQIENLLSPEGSKKNPARTCRDIRLSHPEWSSGFYWIDPNQGCTMDAIKAFCDFSTGQTCIHPHPESIPRKNWYRSSQEKKHTWFGETINSGTEFAYNDETLSPQSMATQLAFMRLLANQAVQNITYHCKNSIAYMDAENGNLKKAVLLQGSNDVELRAEGNSRFTFSVLEDGCSRHTGQWSKTVIEYRTNKPSRLPILDIAPLDIGGADQEFGLDIGPVCFK

Target ORF information:

RefSeq Version	XM_005159713.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio collagen, type I, alpha 2 (col1a2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159713.3

ORF Insert Sequence:

ATGCTCAGCT TTGTGGATAC CCGGATTCTG TTGCTGCTTG CAGTGACTTC GTACCTAGCA 
TCATGTCAAT CGGGCCTCAA GGGACCAAAA GGACCCAGAG GTGAGAGGGG TCCTAAGGGA 
CCTGATGGAA AACCCGGCCG ACCTGGACTT CCTGGCCCTG CTGGCCCTCC TGGCCCCCCT 
GGTCTTGGTG GAAACTTCGC TGCTCAGTAT GATGGCGCTA AAGGACCTGA CCCCGGCCCT 
GGACCTATGG GTTTGATGGG ACCCAGAGGA CCTTCTGGAT CTCCTGGTGC TCCTGGAGCT 
CAAGGACTGC AAGGACATGC TGGTGAGCCT GGAGAGCCCG GACAGGCTGG AGCTATTGGT 
GCTCGTGGCC CTCCTGGACC CCCTGGCAAA AATGGTGAAG ATGGCAACAA TGGCAGACCT 
GGAAAGCCTG GAGACAGAGG AGTCCTTGGA GCTCAGGGTG CCCGTGGTTT CCCAGGAACT 
CCTGGACTTC CTGGCATGAA GGGACACAGA GGTTACAATG GTATTGATGG ACGCAAAGGA 
GAGCCTGGCG CAGCTGGTGC CAAGGGTGAA AATGGTGCCG CTGGATCAAA TGGAACCCCC 
GGACAGAGAG GTGGTCGTGG TCTTCCTGGT GAGAGAGGTC GTGTTGGCCC TGCTGGCCCA 
GCTGGTGCTC GCGGTGCTGA TGGTAACACT GGTCCTGCTG GCCCTGCTGG ACCTTTGGGC 
TCAGCTGGTC CTCCTGGTTT CCCTGGTGCA CCTGGACCCA AGGGAGAACT TGGACCCGCT 
GGTCCCACTG GACCATCTGG AGCTCAGGGA CAAAGAGGAG AGCCAGGCCC GAACGGTGCC 
GTTGGCCCCG TTGGACCCCC TGGTAACCCT GGTGCTAATG GTATCAATGG TGCTAAAGGA 
GCAGCTGGTC TTCCTGGAAT TGCTGGTGCC CCTGGTTTCC CTGGCCCTAG AGGAGGCCCT 
GGCCCACAGG GACCTAGTGG AGCTTCTGGT CCAAGAGGTC TTGGTGGTGA CCCCGGACCT 
GTTGGAGTGA AGGGAGATTC TGGTGTGAAG GGAGAGCCTG GTAGTGCTGG TCCTCAGGGG 
CCTCCTGGTC CTTCTGGTGA GGAGGGCAAG AGAGGGTCAA CTGGAGAGCA GGGACCTACT 
GGACCTTTGG GACTGCGTGG ACCTAGAGGA GCCGCTGGTA CTCGTGGTCT GCCTGGTCTG 
GCTGGCAGGT CAGGCCCAAT GGGCATGCCT GGTCCTCGTG GTGGTGTTGG TGCACCTGGA 
GCCCGTGGAC CTCCTGGAGA TGCTGGTCGT GCTGGTGAGG CTGGTCTTGT TGGAGCCAGA 
GGTCTCCCTG GTAGCCCTGG CAGTTCTGGA CCCCCTGGAA AGGAAGGACC TTCTGGCGAG 
GCTGGCAAGC CTGGTGAGAA GGGACCTGTT GGCCCTACTG GACTGAGAGG CAGCCCTGGT 
CCTGATGGTA ACAATGGTCC TGCTGGTCCT GTTGGACTTG CTGGTGCCCC TGGTGAGAAA 
GGAGAGCAGG GACCCTCTGG TGCTCCTGGT TTCCAGGGTC TTCCTGGCCC CGCTGGACCT 
GTTGGTGAGG CTGGCAAGCC TGGTGATAGA GGTATCCCTG GAGATCAAGG TGTATCAGGA 
CCTGCTGGTG TGAAGGGAGA GCGTGGTAAC CCTGGACCTG CTGGTGCTGC TGGCGCTCAG 
GGACCCATTG GTGCTCGTGG ACCTTCTGGT ACTCCTGGCC CTGATGGAAA CAAGGGAGAG 
CCTGGTGCAG TTGGACCTGC TGGTGCCCCT GGACCACAGG GAGCTGCTGG TATGCCTGGT 
GAGCGTGGTG CTGCTGGCAC CCCTGGAGCT AAGGGAGAGA AGGGTGAGGC TGGATACAGA 
GGACTGGAGG GCAATGCTGG AAAAGATGGT GCCCGTGGTG CTCCTGGACC CAGTGGACCC 
CCTGGACCTG CTGGTGCTAA TGGTGACAAG GGTGAGACTG GCTCCTTTGG TCCTCCTGGA 
CCTGCTGGTC CTCGTGGTGC CCCTGGTGAG CGTGGTGAGT CTGGCCCTGC TGGACCTTCC 
GGTTTTGCTG GACCCCCTGG TGCTGATGGC CAGACTGGAC CAAGAGGTGA GAAAGGACCT 
GCTGGTGGAA AGGGTGACGC TGGACCCGCT GGCCCTGCCG GTCCCGCTGG AAATACTGGA 
CCCCTTGGTC CTTCTGGTCC TGTTGGCCCA CCTGGTGCCC GTGGTGACAG TGGTCCCACT 
GGTCTGACTG GCTTCCCTGG TGCTCCTGGT AGAGTTGGAC CTCCTGGTCC TGCTGGTATT 
GTTGGACCTG CTGGTCTCAC TGGTCCCGCT GGAAAGGATG GCCCACGTGG TCCTCGTGGT 
GATGTTGGCC CTGCTGGTCC CCCAGGAGAG AATGGAATGA TTGGACCTCT TGGTCTGGCT 
GGTGAGAAGG GACCCCCTGG AGAGGCTGGT GCACCTGGAG CACCTGGACC TGCTGGGCCT 
CAGGGTCAGC TGGGATCACA GGGATTCAAT GGTCTTCCTG GCTCCAGAGG TGACCGTGGT 
CTTCCTGGTA TCCCCGGATC AGTTGGTGAA CCTGGAAGAG TTGGCCCTGC TGGTGCCCCT 
GGTGCCCGTG GACCCGGTGG CAACATTGGC ATGCCTGGTA TGACTGGTCC TCAGGGTGAG 
GCTGGACGTG AGGGAAGCCC TGGCAATGAT GGACCTCCTG GCCGTCCTGG TGCTGCTGGA 
ATTAAGGGTG ACCGTGGTGA GCCTGGTTCC CCTGGAACTG CTGGACCTGT TGGTGCTCCT 
GGACCCAATG GACCATCAGG CGCTGTTGGC AGACCTGGAA ACCGTGGTGA ATCTGGTCCT 
TCTGGTCCTA CTGGCGCTGT TGGACCTGCT GGAGCAAGAG GTGCACCTGG CCCTGCTGGA 
CCTCGTGGTG AGAAGGGTGT TGCTGGAGAA AAAGGAGACA GGGGTATGAA GGGACTTCGT 
GGACATCCTG GTCTGCAGGG AATGCCCGGA CCTAACGGTC CTTCTGGTGA CAGTGGGCCT 
GCTGGCATTG CTGGACCTTC CGGTCCAAGA GGCCCAGCTG GTCCCAATGG CCCCGCTGGT 
AAAGATGGTT CAAATGGCAT GCCTGGTGCA ATTGGACCCC CAGGACACCG TGGTCCCGCT 
GGACATGTTG GACCTGCTGG TCCTCCTGGA TCTCCTGGTC TTCCCGGACC CCCTGGCCCA 
TCTGGAGGTG GATATGACAC ATCTGGAGGC TATGATGAGT ACAGAGCTGA CCAGGCCTCT 
CTTAGAGCCA AGGATTACGA GGTGGATGCC ACCATCAAGT CCTTGAACAC TCAGATTGAG 
AATCTGCTTT CCCCTGAGGG CTCAAAGAAG AACCCCGCTC GTACTTGCCG TGACATCAGG 
CTCAGCCACC CAGAATGGAG CAGCGGTTTC TACTGGATCG ACCCCAACCA GGGCTGCACA 
ATGGATGCCA TCAAGGCCTT CTGCGACTTC TCTACTGGCC AGACCTGCAT CCACCCCCAC 
CCAGAGAGCA TCCCACGCAA GAACTGGTAC AGAAGCTCCC AGGAGAAGAA ACACACCTGG 
TTTGGCGAGA CTATCAACAG TGGTACTGAG TTTGCTTACA ATGACGAGAC CCTGAGCCCA 
CAATCCATGG CTACTCAGCT GGCCTTCATG CGTCTACTGG CCAACCAGGC AGTCCAGAAC 
ATCACCTACC ACTGCAAGAA CAGCATCGCC TACATGGATG CTGAGAATGG AAACCTGAAG 
AAGGCTGTGT TGCTGCAGGG TTCCAATGAT GTTGAGCTCA GGGCGGAGGG CAACAGCCGC 
TTCACTTTCA GCGTCCTAGA GGATGGCTGC AGTAGACACA CTGGCCAGTG GAGCAAGACA 
GTCATTGAAT ACAGAACAAA TAAACCATCT CGCCTTCCCA TCCTCGACAT TGCACCTTTG 
GACATTGGTG GCGCAGATCA AGAGTTTGGT TTGGACATTG GCCCAGTCTG TTTCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col1a2 ( XM_005159713.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005159770.1 Danio rerio collagen, type I, alpha 2 (col1a2), transcript variant X1, mRNA.

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CloneID	ODa24381
Clone ID Related Accession (Same CDS sequence)	XM_005159713.3 , XM_005159713.4
Accession Version	XM_005159713.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3960bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	collagen alpha-2(I) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005159713.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAGCT TTGTGGATAC CCGGATTCTG TTGCTGCTTG CAGTGACTTC GTACCTAGCA 
TCATGTCAAT CGGGCCTCAA GGGACCAAAA GGACCCAGAG GTGAGAGGGG TCCTAAGGGA 
CCTGATGGAA AACCCGGCCG ACCTGGACTT CCTGGCCCTG CTGGCCCTCC TGGCCCCCCT 
GGTCTTGGTG GAAACTTCGC TGCTCAGTAT GATGGCGCTA AAGGACCTGA CCCCGGCCCT 
GGACCTATGG GTTTGATGGG ACCCAGAGGA CCTTCTGGAT CTCCTGGTGC TCCTGGAGCT 
CAAGGACTGC AAGGACATGC TGGTGAGCCT GGAGAGCCCG GACAGGCTGG AGCTATTGGT 
GCTCGTGGCC CTCCTGGACC CCCTGGCAAA AATGGTGAAG ATGGCAACAA TGGCAGACCT 
GGAAAGCCTG GAGACAGAGG AGTCCTTGGA GCTCAGGGTG CCCGTGGTTT CCCAGGAACT 
CCTGGACTTC CTGGCATGAA GGGACACAGA GGTTACAATG GTATTGATGG ACGCAAAGGA 
GAGCCTGGCG CAGCTGGTGC CAAGGGTGAA AATGGTGCCG CTGGATCAAA TGGAACCCCC 
GGACAGAGAG GTGGTCGTGG TCTTCCTGGT GAGAGAGGTC GTGTTGGCCC TGCTGGCCCA 
GCTGGTGCTC GCGGTGCTGA TGGTAACACT GGTCCTGCTG GCCCTGCTGG ACCTTTGGGC 
TCAGCTGGTC CTCCTGGTTT CCCTGGTGCA CCTGGACCCA AGGGAGAACT TGGACCCGCT 
GGTCCCACTG GACCATCTGG AGCTCAGGGA CAAAGAGGAG AGCCAGGCCC GAACGGTGCC 
GTTGGCCCCG TTGGACCCCC TGGTAACCCT GGTGCTAATG GTATCAATGG TGCTAAAGGA 
GCAGCTGGTC TTCCTGGAAT TGCTGGTGCC CCTGGTTTCC CTGGCCCTAG AGGAGGCCCT 
GGCCCACAGG GACCTAGTGG AGCTTCTGGT CCAAGAGGTC TTGGTGGTGA CCCCGGACCT 
GTTGGAGTGA AGGGAGATTC TGGTGTGAAG GGAGAGCCTG GTAGTGCTGG TCCTCAGGGG 
CCTCCTGGTC CTTCTGGTGA GGAGGGCAAG AGAGGGTCAA CTGGAGAGCA GGGACCTACT 
GGACCTTTGG GACTGCGTGG ACCTAGAGGA GCCGCTGGTA CTCGTGGTCT GCCTGGTCTG 
GCTGGCAGGT CAGGCCCAAT GGGCATGCCT GGTCCTCGTG GTGGTGTTGG TGCACCTGGA 
GCCCGTGGAC CTCCTGGAGA TGCTGGTCGT GCTGGTGAGG CTGGTCTTGT TGGAGCCAGA 
GGTCTCCCTG GTAGCCCTGG CAGTTCTGGA CCCCCTGGAA AGGAAGGACC TTCTGGCGAG 
GCTGGCAAGC CTGGTGAGAA GGGACCTGTT GGCCCTACTG GACTGAGAGG CAGCCCTGGT 
CCTGATGGTA ACAATGGTCC TGCTGGTCCT GTTGGACTTG CTGGTGCCCC TGGTGAGAAA 
GGAGAGCAGG GACCCTCTGG TGCTCCTGGT TTCCAGGGTC TTCCTGGCCC CGCTGGACCT 
GTTGGTGAGG CTGGCAAGCC TGGTGATAGA GGTATCCCTG GAGATCAAGG TGTATCAGGA 
CCTGCTGGTG TGAAGGGAGA GCGTGGTAAC CCTGGACCTG CTGGTGCTGC TGGCGCTCAG 
GGACCCATTG GTGCTCGTGG ACCTTCTGGT ACTCCTGGCC CTGATGGAAA CAAGGGAGAG 
CCTGGTGCAG TTGGACCTGC TGGTGCCCCT GGACCACAGG GAGCTGCTGG TATGCCTGGT 
GAGCGTGGTG CTGCTGGCAC CCCTGGAGCT AAGGGAGAGA AGGGTGAGGC TGGATACAGA 
GGACTGGAGG GCAATGCTGG AAAAGATGGT GCCCGTGGTG CTCCTGGACC CAGTGGACCC 
CCTGGACCTG CTGGTGCTAA TGGTGACAAG GGTGAGACTG GCTCCTTTGG TCCTCCTGGA 
CCTGCTGGTC CTCGTGGTGC CCCTGGTGAG CGTGGTGAGT CTGGCCCTGC TGGACCTTCC 
GGTTTTGCTG GACCCCCTGG TGCTGATGGC CAGACTGGAC CAAGAGGTGA GAAAGGACCT 
GCTGGTGGAA AGGGTGACGC TGGACCCGCT GGCCCTGCCG GTCCCGCTGG AAATACTGGA 
CCCCTTGGTC CTTCTGGTCC TGTTGGCCCA CCTGGTGCCC GTGGTGACAG TGGTCCCACT 
GGTCTGACTG GCTTCCCTGG TGCTCCTGGT AGAGTTGGAC CTCCTGGTCC TGCTGGTATT 
GTTGGACCTG CTGGTCTCAC TGGTCCCGCT GGAAAGGATG GCCCACGTGG TCCTCGTGGT 
GATGTTGGCC CTGCTGGTCC CCCAGGAGAG AATGGAATGA TTGGACCTCT TGGTCTGGCT 
GGTGAGAAGG GACCCCCTGG AGAGGCTGGT GCACCTGGAG CACCTGGACC TGCTGGGCCT 
CAGGGTCAGC TGGGATCACA GGGATTCAAT GGTCTTCCTG GCTCCAGAGG TGACCGTGGT 
CTTCCTGGTA TCCCCGGATC AGTTGGTGAA CCTGGAAGAG TTGGCCCTGC TGGTGCCCCT 
GGTGCCCGTG GACCCGGTGG CAACATTGGC ATGCCTGGTA TGACTGGTCC TCAGGGTGAG 
GCTGGACGTG AGGGAAGCCC TGGCAATGAT GGACCTCCTG GCCGTCCTGG TGCTGCTGGA 
ATTAAGGGTG ACCGTGGTGA GCCTGGTTCC CCTGGAACTG CTGGACCTGT TGGTGCTCCT 
GGACCCAATG GACCATCAGG CGCTGTTGGC AGACCTGGAA ACCGTGGTGA ATCTGGTCCT 
TCTGGTCCTA CTGGCGCTGT TGGACCTGCT GGAGCAAGAG GTGCACCTGG CCCTGCTGGA 
CCTCGTGGTG AGAAGGGTGT TGCTGGAGAA AAAGGAGACA GGGGTATGAA GGGACTTCGT 
GGACATCCTG GTCTGCAGGG AATGCCCGGA CCTAACGGTC CTTCTGGTGA CAGTGGGCCT 
GCTGGCATTG CTGGACCTTC CGGTCCAAGA GGCCCAGCTG GTCCCAATGG CCCCGCTGGT 
AAAGATGGTT CAAATGGCAT GCCTGGTGCA ATTGGACCCC CAGGACACCG TGGTCCCGCT 
GGACATGTTG GACCTGCTGG TCCTCCTGGA TCTCCTGGTC TTCCCGGACC CCCTGGCCCA 
TCTGGAGGTG GATATGACAC ATCTGGAGGC TATGATGAGT ACAGAGCTGA CCAGGCCTCT 
CTTAGAGCCA AGGATTACGA GGTGGATGCC ACCATCAAGT CCTTGAACAC TCAGATTGAG 
AATCTGCTTT CCCCTGAGGG CTCAAAGAAG AACCCCGCTC GTACTTGCCG TGACATCAGG 
CTCAGCCACC CAGAATGGAG CAGCGGTTTC TACTGGATCG ACCCCAACCA GGGCTGCACA 
ATGGATGCCA TCAAGGCCTT CTGCGACTTC TCTACTGGCC AGACCTGCAT CCACCCCCAC 
CCAGAGAGCA TCCCACGCAA GAACTGGTAC AGAAGCTCCC AGGAGAAGAA ACACACCTGG 
TTTGGCGAGA CTATCAACAG TGGTACTGAG TTTGCTTACA ATGACGAGAC CCTGAGCCCA 
CAATCCATGG CTACTCAGCT GGCCTTCATG CGTCTACTGG CCAACCAGGC AGTCCAGAAC 
ATCACCTACC ACTGCAAGAA CAGCATCGCC TACATGGATG CTGAGAATGG AAACCTGAAG 
AAGGCTGTGT TGCTGCAGGG TTCCAATGAT GTTGAGCTCA GGGCGGAGGG CAACAGCCGC 
TTCACTTTCA GCGTCCTAGA GGATGGCTGC AGTAGACACA CTGGCCAGTG GAGCAAGACA 
GTCATTGAAT ACAGAACAAA TAAACCATCT CGCCTTCCCA TCCTCGACAT TGCACCTTTG 
GACATTGGTG GCGCAGATCA AGAGTTTGGT TTGGACATTG GCCCAGTCTG TTTCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159770.1
CDS	182..4141
Translation	MLSFVDTRILLLLAVTSYLASCQSGLKGPKGPRGERGPKGPDGKPGRPGLPGPAGPPGPPGLGGNFAAQYDGAKGPDPGPGPMGLMGPRGPSGSPGAPGAQGLQGHAGEPGEPGQAGAIGARGPPGPPGKNGEDGNNGRPGKPGDRGVLGAQGARGFPGTPGLPGMKGHRGYNGIDGRKGEPGAAGAKGENGAAGSNGTPGQRGGRGLPGERGRVGPAGPAGARGADGNTGPAGPAGPLGSAGPPGFPGAPGPKGELGPAGPTGPSGAQGQRGEPGPNGAVGPVGPPGNPGANGINGAKGAAGLPGIAGAPGFPGPRGGPGPQGPSGASGPRGLGGDPGPVGVKGDSGVKGEPGSAGPQGPPGPSGEEGKRGSTGEQGPTGPLGLRGPRGAAGTRGLPGLAGRSGPMGMPGPRGGVGAPGARGPPGDAGRAGEAGLVGARGLPGSPGSSGPPGKEGPSGEAGKPGEKGPVGPTGLRGSPGPDGNNGPAGPVGLAGAPGEKGEQGPSGAPGFQGLPGPAGPVGEAGKPGDRGIPGDQGVSGPAGVKGERGNPGPAGAAGAQGPIGARGPSGTPGPDGNKGEPGAVGPAGAPGPQGAAGMPGERGAAGTPGAKGEKGEAGYRGLEGNAGKDGARGAPGPSGPPGPAGANGDKGETGSFGPPGPAGPRGAPGERGESGPAGPSGFAGPPGADGQTGPRGEKGPAGGKGDAGPAGPAGPAGNTGPLGPSGPVGPPGARGDSGPTGLTGFPGAPGRVGPPGPAGIVGPAGLTGPAGKDGPRGPRGDVGPAGPPGENGMIGPLGLAGEKGPPGEAGAPGAPGPAGPQGQLGSQGFNGLPGSRGDRGLPGIPGSVGEPGRVGPAGAPGARGPGGNIGMPGMTGPQGEAGREGSPGNDGPPGRPGAAGIKGDRGEPGSPGTAGPVGAPGPNGPSGAVGRPGNRGESGPSGPTGAVGPAGARGAPGPAGPRGEKGVAGEKGDRGMKGLRGHPGLQGMPGPNGPSGDSGPAGIAGPSGPRGPAGPNGPAGKDGSNGMPGAIGPPGHRGPAGHVGPAGPPGSPGLPGPPGPSGGGYDTSGGYDEYRADQASLRAKDYEVDATIKSLNTQIENLLSPEGSKKNPARTCRDIRLSHPEWSSGFYWIDPNQGCTMDAIKAFCDFSTGQTCIHPHPESIPRKNWYRSSQEKKHTWFGETINSGTEFAYNDETLSPQSMATQLAFMRLLANQAVQNITYHCKNSIAYMDAENGNLKKAVLLQGSNDVELRAEGNSRFTFSVLEDGCSRHTGQWSKTVIEYRTNKPSRLPILDIAPLDIGGADQEFGLDIGPVCFK

Target ORF information:

RefSeq Version	XM_005159713.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio collagen, type I, alpha 2 (col1a2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159713.4

ORF Insert Sequence:

ATGCTCAGCT TTGTGGATAC CCGGATTCTG TTGCTGCTTG CAGTGACTTC GTACCTAGCA 
TCATGTCAAT CGGGCCTCAA GGGACCAAAA GGACCCAGAG GTGAGAGGGG TCCTAAGGGA 
CCTGATGGAA AACCCGGCCG ACCTGGACTT CCTGGCCCTG CTGGCCCTCC TGGCCCCCCT 
GGTCTTGGTG GAAACTTCGC TGCTCAGTAT GATGGCGCTA AAGGACCTGA CCCCGGCCCT 
GGACCTATGG GTTTGATGGG ACCCAGAGGA CCTTCTGGAT CTCCTGGTGC TCCTGGAGCT 
CAAGGACTGC AAGGACATGC TGGTGAGCCT GGAGAGCCCG GACAGGCTGG AGCTATTGGT 
GCTCGTGGCC CTCCTGGACC CCCTGGCAAA AATGGTGAAG ATGGCAACAA TGGCAGACCT 
GGAAAGCCTG GAGACAGAGG AGTCCTTGGA GCTCAGGGTG CCCGTGGTTT CCCAGGAACT 
CCTGGACTTC CTGGCATGAA GGGACACAGA GGTTACAATG GTATTGATGG ACGCAAAGGA 
GAGCCTGGCG CAGCTGGTGC CAAGGGTGAA AATGGTGCCG CTGGATCAAA TGGAACCCCC 
GGACAGAGAG GTGGTCGTGG TCTTCCTGGT GAGAGAGGTC GTGTTGGCCC TGCTGGCCCA 
GCTGGTGCTC GCGGTGCTGA TGGTAACACT GGTCCTGCTG GCCCTGCTGG ACCTTTGGGC 
TCAGCTGGTC CTCCTGGTTT CCCTGGTGCA CCTGGACCCA AGGGAGAACT TGGACCCGCT 
GGTCCCACTG GACCATCTGG AGCTCAGGGA CAAAGAGGAG AGCCAGGCCC GAACGGTGCC 
GTTGGCCCCG TTGGACCCCC TGGTAACCCT GGTGCTAATG GTATCAATGG TGCTAAAGGA 
GCAGCTGGTC TTCCTGGAAT TGCTGGTGCC CCTGGTTTCC CTGGCCCTAG AGGAGGCCCT 
GGCCCACAGG GACCTAGTGG AGCTTCTGGT CCAAGAGGTC TTGGTGGTGA CCCCGGACCT 
GTTGGAGTGA AGGGAGATTC TGGTGTGAAG GGAGAGCCTG GTAGTGCTGG TCCTCAGGGG 
CCTCCTGGTC CTTCTGGTGA GGAGGGCAAG AGAGGGTCAA CTGGAGAGCA GGGACCTACT 
GGACCTTTGG GACTGCGTGG ACCTAGAGGA GCCGCTGGTA CTCGTGGTCT GCCTGGTCTG 
GCTGGCAGGT CAGGCCCAAT GGGCATGCCT GGTCCTCGTG GTGGTGTTGG TGCACCTGGA 
GCCCGTGGAC CTCCTGGAGA TGCTGGTCGT GCTGGTGAGG CTGGTCTTGT TGGAGCCAGA 
GGTCTCCCTG GTAGCCCTGG CAGTTCTGGA CCCCCTGGAA AGGAAGGACC TTCTGGCGAG 
GCTGGCAAGC CTGGTGAGAA GGGACCTGTT GGCCCTACTG GACTGAGAGG CAGCCCTGGT 
CCTGATGGTA ACAATGGTCC TGCTGGTCCT GTTGGACTTG CTGGTGCCCC TGGTGAGAAA 
GGAGAGCAGG GACCCTCTGG TGCTCCTGGT TTCCAGGGTC TTCCTGGCCC CGCTGGACCT 
GTTGGTGAGG CTGGCAAGCC TGGTGATAGA GGTATCCCTG GAGATCAAGG TGTATCAGGA 
CCTGCTGGTG TGAAGGGAGA GCGTGGTAAC CCTGGACCTG CTGGTGCTGC TGGCGCTCAG 
GGACCCATTG GTGCTCGTGG ACCTTCTGGT ACTCCTGGCC CTGATGGAAA CAAGGGAGAG 
CCTGGTGCAG TTGGACCTGC TGGTGCCCCT GGACCACAGG GAGCTGCTGG TATGCCTGGT 
GAGCGTGGTG CTGCTGGCAC CCCTGGAGCT AAGGGAGAGA AGGGTGAGGC TGGATACAGA 
GGACTGGAGG GCAATGCTGG AAAAGATGGT GCCCGTGGTG CTCCTGGACC CAGTGGACCC 
CCTGGACCTG CTGGTGCTAA TGGTGACAAG GGTGAGACTG GCTCCTTTGG TCCTCCTGGA 
CCTGCTGGTC CTCGTGGTGC CCCTGGTGAG CGTGGTGAGT CTGGCCCTGC TGGACCTTCC 
GGTTTTGCTG GACCCCCTGG TGCTGATGGC CAGACTGGAC CAAGAGGTGA GAAAGGACCT 
GCTGGTGGAA AGGGTGACGC TGGACCCGCT GGCCCTGCCG GTCCCGCTGG AAATACTGGA 
CCCCTTGGTC CTTCTGGTCC TGTTGGCCCA CCTGGTGCCC GTGGTGACAG TGGTCCCACT 
GGTCTGACTG GCTTCCCTGG TGCTCCTGGT AGAGTTGGAC CTCCTGGTCC TGCTGGTATT 
GTTGGACCTG CTGGTCTCAC TGGTCCCGCT GGAAAGGATG GCCCACGTGG TCCTCGTGGT 
GATGTTGGCC CTGCTGGTCC CCCAGGAGAG AATGGAATGA TTGGACCTCT TGGTCTGGCT 
GGTGAGAAGG GACCCCCTGG AGAGGCTGGT GCACCTGGAG CACCTGGACC TGCTGGGCCT 
CAGGGTCAGC TGGGATCACA GGGATTCAAT GGTCTTCCTG GCTCCAGAGG TGACCGTGGT 
CTTCCTGGTA TCCCCGGATC AGTTGGTGAA CCTGGAAGAG TTGGCCCTGC TGGTGCCCCT 
GGTGCCCGTG GACCCGGTGG CAACATTGGC ATGCCTGGTA TGACTGGTCC TCAGGGTGAG 
GCTGGACGTG AGGGAAGCCC TGGCAATGAT GGACCTCCTG GCCGTCCTGG TGCTGCTGGA 
ATTAAGGGTG ACCGTGGTGA GCCTGGTTCC CCTGGAACTG CTGGACCTGT TGGTGCTCCT 
GGACCCAATG GACCATCAGG CGCTGTTGGC AGACCTGGAA ACCGTGGTGA ATCTGGTCCT 
TCTGGTCCTA CTGGCGCTGT TGGACCTGCT GGAGCAAGAG GTGCACCTGG CCCTGCTGGA 
CCTCGTGGTG AGAAGGGTGT TGCTGGAGAA AAAGGAGACA GGGGTATGAA GGGACTTCGT 
GGACATCCTG GTCTGCAGGG AATGCCCGGA CCTAACGGTC CTTCTGGTGA CAGTGGGCCT 
GCTGGCATTG CTGGACCTTC CGGTCCAAGA GGCCCAGCTG GTCCCAATGG CCCCGCTGGT 
AAAGATGGTT CAAATGGCAT GCCTGGTGCA ATTGGACCCC CAGGACACCG TGGTCCCGCT 
GGACATGTTG GACCTGCTGG TCCTCCTGGA TCTCCTGGTC TTCCCGGACC CCCTGGCCCA 
TCTGGAGGTG GATATGACAC ATCTGGAGGC TATGATGAGT ACAGAGCTGA CCAGGCCTCT 
CTTAGAGCCA AGGATTACGA GGTGGATGCC ACCATCAAGT CCTTGAACAC TCAGATTGAG 
AATCTGCTTT CCCCTGAGGG CTCAAAGAAG AACCCCGCTC GTACTTGCCG TGACATCAGG 
CTCAGCCACC CAGAATGGAG CAGCGGTTTC TACTGGATCG ACCCCAACCA GGGCTGCACA 
ATGGATGCCA TCAAGGCCTT CTGCGACTTC TCTACTGGCC AGACCTGCAT CCACCCCCAC 
CCAGAGAGCA TCCCACGCAA GAACTGGTAC AGAAGCTCCC AGGAGAAGAA ACACACCTGG 
TTTGGCGAGA CTATCAACAG TGGTACTGAG TTTGCTTACA ATGACGAGAC CCTGAGCCCA 
CAATCCATGG CTACTCAGCT GGCCTTCATG CGTCTACTGG CCAACCAGGC AGTCCAGAAC 
ATCACCTACC ACTGCAAGAA CAGCATCGCC TACATGGATG CTGAGAATGG AAACCTGAAG 
AAGGCTGTGT TGCTGCAGGG TTCCAATGAT GTTGAGCTCA GGGCGGAGGG CAACAGCCGC 
TTCACTTTCA GCGTCCTAGA GGATGGCTGC AGTAGACACA CTGGCCAGTG GAGCAAGACA 
GTCATTGAAT ACAGAACAAA TAAACCATCT CGCCTTCCCA TCCTCGACAT TGCACCTTTG 
GACATTGGTG GCGCAGATCA AGAGTTTGGT TTGGACATTG GCCCAGTCTG TTTCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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col1a2 cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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