PTPN4A cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	PTPN4A
Entrez Gene ID	368448
Full Name	protein tyrosine phosphatase, non-receptor type 4a
Synonyms	ptpn4,si:busm1-37o5.1,si:dz37o5.1
General protein information	Preferred Names protein tyrosine phosphatase, non-receptor type 4a Names tyrosine-protein phosphatase non-receptor type 4 protein tyrosine phosphatase non-receptor type 4 protein tyrosine phosphatase, non-receptor type 4 (megakaryocyte)
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001007200.1	NP_001007201.1	tyrosine-protein phosphatase non-receptor type 4
XM_005167672.3	XP_005167729.1	tyrosine-protein phosphatase non-receptor type 4 isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ptpn4	NP_064317.2
Danio rerio (zebrafish)	ptpn4a	NP_001007201.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007130	XP_308630.4
	PTPN4	XP_001084627.1
Bos taurus (cattle)	PTPN4	NP_001179875.1
Rattus norvegicus (Norway rat)	Ptpn4	NP_001093949.1
Gallus gallus (chicken)	PTPN4	XP_004942950.1
Drosophila melanogaster (fruit fly)	Ptpmeg	NP_728523.2
Caenorhabditis elegans (roundworm)	ptp-1	NP_497732.1
Pan troglodytes (chimpanzee)	PTPN4	XP_525902.2
Canis lupus familiaris (dog)	PTPN4	XP_541002.2
Homo sapiens (human)	PTPN4	NP_002821.1
Caenorhabditis elegans (roundworm)	ptp-1	NP_497733.1

Gene Ontology
Bibliography

Related articles in PubMed

Differential oxidation of protein-tyrosine phosphatases during zebrafish caudal fin regeneration.
Wu W, Hale AJ, Lemeer S, den Hertog J
Scientific reports7(1)8460(2017 Aug)

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Zebrafish as a model to study PTPs during development.
Paardekooper Overman J, den Hertog J
Methods (San Diego, Calif.)65(2)247-53(2014 Jan)

Identification and expression of the family of classical protein-tyrosine phosphatases in zebrafish.
van Eekelen M, Overvoorde J, van Rooijen C, den Hertog J
PloS one5(9)e12573(2010 Sep)

Zebrafish mosaic eyes is a novel FERM protein required for retinal lamination and retinal pigmented epithelial tight junction formation.
Jensen AM, Westerfield M
Current biology : CB14(8)711-7(2004 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PTPN4A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PTPN4A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa01842	NM_001007200.1 Latest version!	Danio rerio protein tyrosine phosphatase non-receptor type 4a (ptpn4a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa15221	XM_005167672.3 Latest version!	Danio rerio protein tyrosine phosphatase, non-receptor type 4a (ptpn4a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PTPN4A ( NM_001007200.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001007201.1 Danio rerio protein tyrosine phosphatase non-receptor type 4a (ptpn4a), mRNA.

Price & Availability↑

CloneID	ODa01842
Clone ID Related Accession (Same CDS sequence)	NM_001007200.1
Accession Version	NM_001007200.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2766bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-07
Organism	Danio rerio(zebrafish)
Product	tyrosine-protein phosphatase non-receptor type 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL672097.5. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN01765705 [ECO:0000350] ##Evidence-Data-END##

ATGACCGCAC GCTTCCGGTT GCCTGCTGGC AGATCCTACA ATGTCCGGAC GACGGAGCTG 
GCACGCGACA GACAGCGCAC CGAGGTGGTC TGCAACATTC TTCTTTTGGA CAACACTGTC 
CAGCCTTTTA AAGTCAATAA GCACGATCAA GGACAGATCC TACTGGACGC TGTCTTCAAG 
CACCTGGAAC TAACCGAGAG AGACTATTTT GGGTTGCACC TGGCCGACGA GCCCTCTGAT 
AGTCCGAGGT GGCTGGATCC AAATAAACCC ATAAGGAAGC AATTAAAAAG GGGCTCCCCA 
CATCATCTGA ACTTCCGGGT GAAATTCTTT GTGAGTGACC CCAACAAACT TCAGGAAGAA 
TACACACGGT ACCAGTATTT TCTACAGATT AAGCAAGACA TACTCAGTGG AAGACTGCCC 
TGTCCGCACA ATACTGCCGC ACTCCTTGCA TCGTATGCCG TTCAGTCCGA GCTGGGTGAT 
TATAGCGAGG CTGAGAATTT GCCAGGATAC CTGTCTGAGT TCTTCTTCAT CCCCAACCAG 
CCTCAGGGCT TTGAGAAAGA TGTCGCCAAA CATCACCAGC AACACAATGG ACTTAACCCA 
GCACAGTCTG AATTCAATTA CCTGAACACA GCACGAACAT TGGATCTCTA TGGAGTTGAA 
CTACACTATG CAAGGGATCA AAGCAACACT GAGATTTATG TGGGCGTGTT GTCAGCTGGC 
ATTGGCATCT ACAAGAACAG GGTACGAATC AACTATTTTC CATGGTTAAA GATTGTGAAA 
ATATCTTTTA AGTGCAAACA GTTTTTCATC CAGCTAAGAA AAGAGGCGCA TGATACAAGA 
GATACATTGC TCGGTTTCAA CATGGTAAAC TACAGGGCCT GTAAGAACCT CTGGAAAACC 
TGTGTTGAAC ACCACACCTT CTTTCGGCTG GATCGACCCG TTCCTCCACA GAAGAACTTT 
TTTGCGCACT ACTTCAGTTT GGGCTCCAGG TTTCGTTACT GCGGGAGGAC GGAAGCACAA 
TCTGTTCAGT ATGGCAAGGA GAAGGGAATC AAGGACAGGG TGTTTGCAAG CAAGCCCCTG 
GCCAGAAAGC TGATGGGTGT GACAGACTGG GAAGCAGTGA GCAGGAACTC ACTGTCTGAA 
GAGAGGCTAG AGACCCGCAG CCTACCCACC CGCTCCCCTC CTGGGACACC TAACCACAAC 
TCCTTGTTTG TTCAAGAGGG TCCCCGGCTG CGGCCCTCTT CAATGGGTCA CCTGGTGGAC 
CATGTCATCC ATGCCTCCCC CAGCCTTCCG GTTTTCTCCA ATCACAAATC GGCTTCCTCT 
ACACAAGCTA ACAGCATCAG CCTGGAGTCT ACACCGTCTC CAGAAGGTGT TGAAGGTCCA 
CCACCAGCCC TCCCACCCAA GCAGTCCCGG AAGAACCTGA GCCAGCTCAT ACTGTCCCAT 
TCCCAACAGG ACTTGGACAA CCACATCAAC GAAATGTACG ATGTCCCCAC AGCAACCGAC 
AAGGTCATGC CCAATGGAGT CGTTCCTCAT GACAATCTTG TCCTGATCAA GATGAAGCCG 
GATGAGAACG GGAGATTTGG GTTTAATGTG AAGGGTGGAT CTGATCAAAA GATGCCAATT 
ATTGTGTCTC GAGTAGCGCC TGGAACATCG GCTGATTTGT GTGTCCCCCG ACTGAACGAG 
GGCGATCAGG TTGTGCTGAT CAACGGTCGG GAAATTGCAG AGCATTCACA TGATCAGGTG 
GTCATGTTCA TCAAGGCCAG CTGTGAAAGC CACTCGGGGG AGCTCATTCT GCTGGTGCGG 
CCCAATGCAA TCTATGATGT TGTTGAGGAA AAGCTGGAGA CAGAGCCAGA TTTCCAGTAT 
ATCCCTGAAA AATCTCCTAC AGACCCATCT CACCTCGACC AGGACGCCTG GAGAGACTCA 
ATGCTGTATT TAAAGGAGGG TCTCAACAGC GGTACTATAC TGGCCCAGTT TGATCAATTG 
TATCGTAAAA GGCCTGGAAT GACAATGTCA TGTGCCAAAT TACCTCAGAA TATTTCCAAA 
AACCGCTACA GAGACATCTC ACCTTATGAT GCAACTCGAG TCATTCTGAA AGGCACAGAA 
GACTACATCA ATGCAAATTA CATCAACATG GAAATCCCTG CCTGCAGTCT CATAAACCGC 
TATATCGCCT GCCAGGGGCC TCTGCCCAAC ACGTGCTCTG ATTTTTGGCA GATGACGTGG 
GAGCAGGGCT CTTCTATGGT AGTCATGCTT ACTACTCAGG TCAAGTGCCA TCAGTACTGG 
CCGAACCCCT CAGGTAGCGC TACATACGGC GGCTTTCAGA TTTCCTGCCA GACTGAAGAG 
GGAAACTCTG CTTTCTTGGT TCGGGAAATA ACACTTACTC ACATTGAGAG TGGAGAGAGC 
AGAGAACTGA CCCAAATCCA GTATCTGGCT TGGCCTGACC ACGGTGTACC AGACGATTCA 
ACAGACTTCC TGGATTTTGT GGCTCTGGTC CGGAATAAAC GAGCAGGGAA GGATGAGCCT 
GTGGTGGTCC ACTGCAGTGC TGGGATTGGT CGGACCGGAG TTCTCATCAC AATGGAGACA 
GCCATGTGTC TGATGGAGTG CAGTCAGCCG GTGTTTCCAT TAGAAATAGT GAGAACCATG 
CGGGATCAGA GGGCCATGAT GATTCAGACG CCTAGCCAGT TCAAGTTTGT GTGCGAGGCC 
ATACTGAAAG TTTACGAGGA GGGCCTGGTC AAACCGCTCA AGTCCATTCT GTACGAATCC 
AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001007201.1
CDS	14..2779
Translation	MTARFRLPAGRSYNVRTTELARDRQRTEVVCNILLLDNTVQPFKVNKHDQGQILLDAVFKHLELTERDYFGLHLADEPSDSPRWLDPNKPIRKQLKRGSPHHLNFRVKFFVSDPNKLQEEYTRYQYFLQIKQDILSGRLPCPHNTAALLASYAVQSELGDYSEAENLPGYLSEFFFIPNQPQGFEKDVAKHHQQHNGLNPAQSEFNYLNTARTLDLYGVELHYARDQSNTEIYVGVLSAGIGIYKNRVRINYFPWLKIVKISFKCKQFFIQLRKEAHDTRDTLLGFNMVNYRACKNLWKTCVEHHTFFRLDRPVPPQKNFFAHYFSLGSRFRYCGRTEAQSVQYGKEKGIKDRVFASKPLARKLMGVTDWEAVSRNSLSEERLETRSLPTRSPPGTPNHNSLFVQEGPRLRPSSMGHLVDHVIHASPSLPVFSNHKSASSTQANSISLESTPSPEGVEGPPPALPPKQSRKNLSQLILSHSQQDLDNHINEMYDVPTATDKVMPNGVVPHDNLVLIKMKPDENGRFGFNVKGGSDQKMPIIVSRVAPGTSADLCVPRLNEGDQVVLINGREIAEHSHDQVVMFIKASCESHSGELILLVRPNAIYDVVEEKLETEPDFQYIPEKSPTDPSHLDQDAWRDSMLYLKEGLNSGTILAQFDQLYRKRPGMTMSCAKLPQNISKNRYRDISPYDATRVILKGTEDYINANYINMEIPACSLINRYIACQGPLPNTCSDFWQMTWEQGSSMVVMLTTQVKCHQYWPNPSGSATYGGFQISCQTEEGNSAFLVREITLTHIESGESRELTQIQYLAWPDHGVPDDSTDFLDFVALVRNKRAGKDEPVVVHCSAGIGRTGVLITMETAMCLMECSQPVFPLEIVRTMRDQRAMMIQTPSQFKFVCEAILKVYEEGLVKPLKSILYESN

Target ORF information:

RefSeq Version	NM_001007200.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein tyrosine phosphatase non-receptor type 4a (ptpn4a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001007200.1

ORF Insert Sequence:

ATGACCGCAC GCTTCCGGTT GCCTGCTGGC AGATCCTACA ATGTCCGGAC GACGGAGCTG 
GCACGCGACA GACAGCGCAC CGAGGTGGTC TGCAACATTC TTCTTTTGGA CAACACTGTC 
CAGCCTTTTA AAGTCAATAA GCACGATCAA GGACAGATCC TACTGGACGC TGTCTTCAAG 
CACCTGGAAC TAACCGAGAG AGACTATTTT GGGTTGCACC TGGCCGACGA GCCCTCTGAT 
AGTCCGAGGT GGCTGGATCC AAATAAACCC ATAAGGAAGC AATTAAAAAG GGGCTCCCCA 
CATCATCTGA ACTTCCGGGT GAAATTCTTT GTGAGTGACC CCAACAAACT TCAGGAAGAA 
TACACACGGT ACCAGTATTT TCTACAGATT AAGCAAGACA TACTCAGTGG AAGACTGCCC 
TGTCCGCACA ATACTGCCGC ACTCCTTGCA TCGTATGCCG TTCAGTCCGA GCTGGGTGAT 
TATAGCGAGG CTGAGAATTT GCCAGGATAC CTGTCTGAGT TCTTCTTCAT CCCCAACCAG 
CCTCAGGGCT TTGAGAAAGA TGTCGCCAAA CATCACCAGC AACACAATGG ACTTAACCCA 
GCACAGTCTG AATTCAATTA CCTGAACACA GCACGAACAT TGGATCTCTA TGGAGTTGAA 
CTACACTATG CAAGGGATCA AAGCAACACT GAGATTTATG TGGGCGTGTT GTCAGCTGGC 
ATTGGCATCT ACAAGAACAG GGTACGAATC AACTATTTTC CATGGTTAAA GATTGTGAAA 
ATATCTTTTA AGTGCAAACA GTTTTTCATC CAGCTAAGAA AAGAGGCGCA TGATACAAGA 
GATACATTGC TCGGTTTCAA CATGGTAAAC TACAGGGCCT GTAAGAACCT CTGGAAAACC 
TGTGTTGAAC ACCACACCTT CTTTCGGCTG GATCGACCCG TTCCTCCACA GAAGAACTTT 
TTTGCGCACT ACTTCAGTTT GGGCTCCAGG TTTCGTTACT GCGGGAGGAC GGAAGCACAA 
TCTGTTCAGT ATGGCAAGGA GAAGGGAATC AAGGACAGGG TGTTTGCAAG CAAGCCCCTG 
GCCAGAAAGC TGATGGGTGT GACAGACTGG GAAGCAGTGA GCAGGAACTC ACTGTCTGAA 
GAGAGGCTAG AGACCCGCAG CCTACCCACC CGCTCCCCTC CTGGGACACC TAACCACAAC 
TCCTTGTTTG TTCAAGAGGG TCCCCGGCTG CGGCCCTCTT CAATGGGTCA CCTGGTGGAC 
CATGTCATCC ATGCCTCCCC CAGCCTTCCG GTTTTCTCCA ATCACAAATC GGCTTCCTCT 
ACACAAGCTA ACAGCATCAG CCTGGAGTCT ACACCGTCTC CAGAAGGTGT TGAAGGTCCA 
CCACCAGCCC TCCCACCCAA GCAGTCCCGG AAGAACCTGA GCCAGCTCAT ACTGTCCCAT 
TCCCAACAGG ACTTGGACAA CCACATCAAC GAAATGTACG ATGTCCCCAC AGCAACCGAC 
AAGGTCATGC CCAATGGAGT CGTTCCTCAT GACAATCTTG TCCTGATCAA GATGAAGCCG 
GATGAGAACG GGAGATTTGG GTTTAATGTG AAGGGTGGAT CTGATCAAAA GATGCCAATT 
ATTGTGTCTC GAGTAGCGCC TGGAACATCG GCTGATTTGT GTGTCCCCCG ACTGAACGAG 
GGCGATCAGG TTGTGCTGAT CAACGGTCGG GAAATTGCAG AGCATTCACA TGATCAGGTG 
GTCATGTTCA TCAAGGCCAG CTGTGAAAGC CACTCGGGGG AGCTCATTCT GCTGGTGCGG 
CCCAATGCAA TCTATGATGT TGTTGAGGAA AAGCTGGAGA CAGAGCCAGA TTTCCAGTAT 
ATCCCTGAAA AATCTCCTAC AGACCCATCT CACCTCGACC AGGACGCCTG GAGAGACTCA 
ATGCTGTATT TAAAGGAGGG TCTCAACAGC GGTACTATAC TGGCCCAGTT TGATCAATTG 
TATCGTAAAA GGCCTGGAAT GACAATGTCA TGTGCCAAAT TACCTCAGAA TATTTCCAAA 
AACCGCTACA GAGACATCTC ACCTTATGAT GCAACTCGAG TCATTCTGAA AGGCACAGAA 
GACTACATCA ATGCAAATTA CATCAACATG GAAATCCCTG CCTGCAGTCT CATAAACCGC 
TATATCGCCT GCCAGGGGCC TCTGCCCAAC ACGTGCTCTG ATTTTTGGCA GATGACGTGG 
GAGCAGGGCT CTTCTATGGT AGTCATGCTT ACTACTCAGG TCAAGTGCCA TCAGTACTGG 
CCGAACCCCT CAGGTAGCGC TACATACGGC GGCTTTCAGA TTTCCTGCCA GACTGAAGAG 
GGAAACTCTG CTTTCTTGGT TCGGGAAATA ACACTTACTC ACATTGAGAG TGGAGAGAGC 
AGAGAACTGA CCCAAATCCA GTATCTGGCT TGGCCTGACC ACGGTGTACC AGACGATTCA 
ACAGACTTCC TGGATTTTGT GGCTCTGGTC CGGAATAAAC GAGCAGGGAA GGATGAGCCT 
GTGGTGGTCC ACTGCAGTGC TGGGATTGGT CGGACCGGAG TTCTCATCAC AATGGAGACA 
GCCATGTGTC TGATGGAGTG CAGTCAGCCG GTGTTTCCAT TAGAAATAGT GAGAACCATG 
CGGGATCAGA GGGCCATGAT GATTCAGACG CCTAGCCAGT TCAAGTTTGT GTGCGAGGCC 
ATACTGAAAG TTTACGAGGA GGGCCTGGTC AAACCGCTCA AGTCCATTCT GTACGAATCC 
AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTPN4A ( XM_005167672.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005167729.1 Danio rerio protein tyrosine phosphatase, non-receptor type 4a (ptpn4a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa15221
Clone ID Related Accession (Same CDS sequence)	XM_005167672.3
Accession Version	XM_005167672.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2790bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tyrosine-protein phosphatase non-receptor type 4 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005167672.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGCAC GCTTCCGGTT GCCTGCTGGC AGATCCTACA ATGTCCGGAC GACGGAGCTG 
GCACGCGACA GACAGCGCAC CGAGGTGGTC TGCAACATTC TTCTTTTGGA CAACACTGTC 
CAGCCTTTTA AAGTCAATAA GCACGATCAA GGACAGATCC TACTGGACGC TGTCTTCAAG 
CACCTGGAAC TAACCGAGAG AGACTATTTT GGGTTGCACC TGGCCGACGA GCCCTCTGAT 
AGTCCGAGGT GGCTGGATCC AAATAAACCC ATAAGGAAGC AATTAAAAAG GGGCTCCCCA 
CATCATCTGA ACTTCCGGGT GAAATTCTTT GTGAGTGACC CCAACAAACT TCAGGAAGAA 
TACACACGGT ACCAGTATTT TCTACAGATT AAGCAAGACA TACTCAGTGG AAGACTGCCC 
TGTCCGCACA ATACTGCCGC ACTCCTTGCA TCGTATGCCG TTCAGTCCGA GCTGGGTGAT 
TATAGCGAGG CTGAGAATTT GCCAGGATAC CTGTCTGAGT TCTTCTTCAT CCCCAACCAG 
CCTCAGGGCT TTGAGAAAGA TGTCGCCAAA CATCACCAGC AACACAATGG ACTTAACCCA 
GCACAGTCTG AATTCAATTA CCTGAACACA GCACGAACAT TGGATCTCTA TGGAGTTGAA 
CTACACTATG CAAGGGATCA AAGCAACACT GAGATTTATG TGGGCGTGTT GTCAGCTGGC 
ATTGGCATCT ACAAGAACAG GGTACGAATC AACTATTTTC CATGGTTAAA GATTGTGAAA 
ATATCTTTTA AGTGCAAACA GTTTTTCATC CAGCTAAGAA AAGAGGCGCA TGATACAAGA 
GATACATTGC TCGGTTTCAA CATGGTAAAC TACAGGGCCT GTAAGAACCT CTGGAAAACC 
TGTGTTGAAC ACCACACCTT CTTTCGGCTG GATCGACCCG TTCCTCCACA GAAGAACTTT 
TTTGCGCACT ACTTCAGTTT GGGCTCCAGG TTTCGTTACT GCGGGAGGAC GGAAGCACAA 
TCTGTTCAGT ATGGCAAGGA GAAGGGAATC AAGGACAGGG TGTTTGCAAG ATCACCTAGC 
AAGCCCCTGG CCAGAAAGCT GATGGGTGTG ACAGACTGGG AAGCAGTGAG CAGGAACTCA 
CTGTCTGAAG AGAGGCTAGA GACCCGCAGC CTACCCACCC GCTCCCCTCC TGGGACACCT 
AACCACAACT CCTTGTTTGT TCAAGAGGGT CCCCGGCTGC GGCCCTCTTC AATGGGTCAC 
CTGGTGGACC ATGTCATCCA TGCCTCCCCC AGCCTTCCGG TTTTCTCCAA TCACAAATCG 
GCTTCCTCTA CACAAGCTAA CAGCATCAGC CTGGAGTCTA CACCGTCTCC AGAAGGTGTT 
GAAGGTCCAC CACCAGCCCT CCCACCCAAG CAGTCCCGGA AGAACCTGAG CCAGCTCATA 
CTGTCCCATT CCCAACAGGA CTTGGACAAC CACATCAACG AAATGTACGA TGTCCCCACA 
GCAACCGACA AGGTCATGCC CAATGGAGTC GTTCCTCATG ACAATCTTGT CCTGATCAAG 
ATGAAGCCGG ATGAGAACGG GAGATTTGGG TTTAATGTGA AGGGTGGATC TGATCAAAAG 
ATGCCAATTA TTGTGTCTCG AGTAGCGCCT GGAACATCGG CTGATTTGTG TGTCCCCCGA 
CTGAACGAGG GCGATCAGGT TGTGCTGATC AACGGTCGGG AAATTGCAGA GCATTCACAT 
GATCAGGTGG TCATGTTCAT CAAGGCCAGC TGTGAAAGCC ACTCGGGGGA GCTCATTCTG 
CTGGTGCGGC CCAATGCAAT CTATGATGTT GTTGAGGAAA AGCTGGAGAC AGAGCCAGAT 
TTCCAGTATA TCCCTGAAAA ATCTCCTACA GACCCATCTC ACCTCGACCA GGACGCCTGG 
AGAGACTCAA TGCTGTATTT AAAGGAGGGT CTCAACAGCG GTACTATACT GGCCCAGTTT 
GATCAATTGT ATCGTAAAAG GCCTGGAATG ACAATGTCAT GTGCCAAATT ACCTCAGAAT 
ATTTCCAAAA ACCGCTACAG AGACATCTCA CCTTATGATG CAACTCGAGT CATTCTGAAA 
GGCACAGAAG ACTACATCAA TGCAAATTAC ATCAACATGG AAATCCCTGC CTGCAGTCTC 
ATAAACCGCT ATATCGCCTG CCAGGGGCCT CTGCCCAACA CGTGCTCTGA TTTTTGGCAG 
ATGACGTGGG AGCAGGGCTC TTCTATGGTA GTCATGCTTA CTACTCAGGT TGAGCGAGGA 
CGTGTCAAGT GCCATCAGTA CTGGCCGAAC CCCTCAGGTA GCGCTACATA CGGCGGCTTT 
CAGATTTCCT GCCAGACTGA AGAGGGAAAC TCTGCTTTCT TGGTTCGGGA AATAACACTT 
ACTCACATTG AGAGTGGAGA GAGCAGAGAA CTGACCCAAA TCCAGTATCT GGCTTGGCCT 
GACCACGGTG TACCAGACGA TTCAACAGAC TTCCTGGATT TTGTGGCTCT GGTCCGGAAT 
AAACGAGCAG GGAAGGATGA GCCTGTGGTG GTCCACTGCA GTGCTGGGAT TGGTCGGACC 
GGAGTTCTCA TCACAATGGA GACAGCCATG TGTCTGATGG AGTGCAGTCA GCCGGTGTTT 
CCATTAGAAA TAGTGAGAAC CATGCGGGAT CAGAGGGCCA TGATGATTCA GACGCCTAGC 
CAGTTCAAGT TTGTGTGCGA GGCCATACTG AAAGTTTACG AGGAGGGCCT GGTCAAACCG 
CTCAAGTCCA TTCTGTACGA ATCCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167729.1
CDS	451..3240
Translation	MTARFRLPAGRSYNVRTTELARDRQRTEVVCNILLLDNTVQPFKVNKHDQGQILLDAVFKHLELTERDYFGLHLADEPSDSPRWLDPNKPIRKQLKRGSPHHLNFRVKFFVSDPNKLQEEYTRYQYFLQIKQDILSGRLPCPHNTAALLASYAVQSELGDYSEAENLPGYLSEFFFIPNQPQGFEKDVAKHHQQHNGLNPAQSEFNYLNTARTLDLYGVELHYARDQSNTEIYVGVLSAGIGIYKNRVRINYFPWLKIVKISFKCKQFFIQLRKEAHDTRDTLLGFNMVNYRACKNLWKTCVEHHTFFRLDRPVPPQKNFFAHYFSLGSRFRYCGRTEAQSVQYGKEKGIKDRVFARSPSKPLARKLMGVTDWEAVSRNSLSEERLETRSLPTRSPPGTPNHNSLFVQEGPRLRPSSMGHLVDHVIHASPSLPVFSNHKSASSTQANSISLESTPSPEGVEGPPPALPPKQSRKNLSQLILSHSQQDLDNHINEMYDVPTATDKVMPNGVVPHDNLVLIKMKPDENGRFGFNVKGGSDQKMPIIVSRVAPGTSADLCVPRLNEGDQVVLINGREIAEHSHDQVVMFIKASCESHSGELILLVRPNAIYDVVEEKLETEPDFQYIPEKSPTDPSHLDQDAWRDSMLYLKEGLNSGTILAQFDQLYRKRPGMTMSCAKLPQNISKNRYRDISPYDATRVILKGTEDYINANYINMEIPACSLINRYIACQGPLPNTCSDFWQMTWEQGSSMVVMLTTQVERGRVKCHQYWPNPSGSATYGGFQISCQTEEGNSAFLVREITLTHIESGESRELTQIQYLAWPDHGVPDDSTDFLDFVALVRNKRAGKDEPVVVHCSAGIGRTGVLITMETAMCLMECSQPVFPLEIVRTMRDQRAMMIQTPSQFKFVCEAILKVYEEGLVKPLKSILYESN

Target ORF information:

RefSeq Version	XM_005167672.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, non-receptor type 4a (ptpn4a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167672.3

ORF Insert Sequence:

ATGACCGCAC GCTTCCGGTT GCCTGCTGGC AGATCCTACA ATGTCCGGAC GACGGAGCTG 
GCACGCGACA GACAGCGCAC CGAGGTGGTC TGCAACATTC TTCTTTTGGA CAACACTGTC 
CAGCCTTTTA AAGTCAATAA GCACGATCAA GGACAGATCC TACTGGACGC TGTCTTCAAG 
CACCTGGAAC TAACCGAGAG AGACTATTTT GGGTTGCACC TGGCCGACGA GCCCTCTGAT 
AGTCCGAGGT GGCTGGATCC AAATAAACCC ATAAGGAAGC AATTAAAAAG GGGCTCCCCA 
CATCATCTGA ACTTCCGGGT GAAATTCTTT GTGAGTGACC CCAACAAACT TCAGGAAGAA 
TACACACGGT ACCAGTATTT TCTACAGATT AAGCAAGACA TACTCAGTGG AAGACTGCCC 
TGTCCGCACA ATACTGCCGC ACTCCTTGCA TCGTATGCCG TTCAGTCCGA GCTGGGTGAT 
TATAGCGAGG CTGAGAATTT GCCAGGATAC CTGTCTGAGT TCTTCTTCAT CCCCAACCAG 
CCTCAGGGCT TTGAGAAAGA TGTCGCCAAA CATCACCAGC AACACAATGG ACTTAACCCA 
GCACAGTCTG AATTCAATTA CCTGAACACA GCACGAACAT TGGATCTCTA TGGAGTTGAA 
CTACACTATG CAAGGGATCA AAGCAACACT GAGATTTATG TGGGCGTGTT GTCAGCTGGC 
ATTGGCATCT ACAAGAACAG GGTACGAATC AACTATTTTC CATGGTTAAA GATTGTGAAA 
ATATCTTTTA AGTGCAAACA GTTTTTCATC CAGCTAAGAA AAGAGGCGCA TGATACAAGA 
GATACATTGC TCGGTTTCAA CATGGTAAAC TACAGGGCCT GTAAGAACCT CTGGAAAACC 
TGTGTTGAAC ACCACACCTT CTTTCGGCTG GATCGACCCG TTCCTCCACA GAAGAACTTT 
TTTGCGCACT ACTTCAGTTT GGGCTCCAGG TTTCGTTACT GCGGGAGGAC GGAAGCACAA 
TCTGTTCAGT ATGGCAAGGA GAAGGGAATC AAGGACAGGG TGTTTGCAAG ATCACCTAGC 
AAGCCCCTGG CCAGAAAGCT GATGGGTGTG ACAGACTGGG AAGCAGTGAG CAGGAACTCA 
CTGTCTGAAG AGAGGCTAGA GACCCGCAGC CTACCCACCC GCTCCCCTCC TGGGACACCT 
AACCACAACT CCTTGTTTGT TCAAGAGGGT CCCCGGCTGC GGCCCTCTTC AATGGGTCAC 
CTGGTGGACC ATGTCATCCA TGCCTCCCCC AGCCTTCCGG TTTTCTCCAA TCACAAATCG 
GCTTCCTCTA CACAAGCTAA CAGCATCAGC CTGGAGTCTA CACCGTCTCC AGAAGGTGTT 
GAAGGTCCAC CACCAGCCCT CCCACCCAAG CAGTCCCGGA AGAACCTGAG CCAGCTCATA 
CTGTCCCATT CCCAACAGGA CTTGGACAAC CACATCAACG AAATGTACGA TGTCCCCACA 
GCAACCGACA AGGTCATGCC CAATGGAGTC GTTCCTCATG ACAATCTTGT CCTGATCAAG 
ATGAAGCCGG ATGAGAACGG GAGATTTGGG TTTAATGTGA AGGGTGGATC TGATCAAAAG 
ATGCCAATTA TTGTGTCTCG AGTAGCGCCT GGAACATCGG CTGATTTGTG TGTCCCCCGA 
CTGAACGAGG GCGATCAGGT TGTGCTGATC AACGGTCGGG AAATTGCAGA GCATTCACAT 
GATCAGGTGG TCATGTTCAT CAAGGCCAGC TGTGAAAGCC ACTCGGGGGA GCTCATTCTG 
CTGGTGCGGC CCAATGCAAT CTATGATGTT GTTGAGGAAA AGCTGGAGAC AGAGCCAGAT 
TTCCAGTATA TCCCTGAAAA ATCTCCTACA GACCCATCTC ACCTCGACCA GGACGCCTGG 
AGAGACTCAA TGCTGTATTT AAAGGAGGGT CTCAACAGCG GTACTATACT GGCCCAGTTT 
GATCAATTGT ATCGTAAAAG GCCTGGAATG ACAATGTCAT GTGCCAAATT ACCTCAGAAT 
ATTTCCAAAA ACCGCTACAG AGACATCTCA CCTTATGATG CAACTCGAGT CATTCTGAAA 
GGCACAGAAG ACTACATCAA TGCAAATTAC ATCAACATGG AAATCCCTGC CTGCAGTCTC 
ATAAACCGCT ATATCGCCTG CCAGGGGCCT CTGCCCAACA CGTGCTCTGA TTTTTGGCAG 
ATGACGTGGG AGCAGGGCTC TTCTATGGTA GTCATGCTTA CTACTCAGGT TGAGCGAGGA 
CGTGTCAAGT GCCATCAGTA CTGGCCGAAC CCCTCAGGTA GCGCTACATA CGGCGGCTTT 
CAGATTTCCT GCCAGACTGA AGAGGGAAAC TCTGCTTTCT TGGTTCGGGA AATAACACTT 
ACTCACATTG AGAGTGGAGA GAGCAGAGAA CTGACCCAAA TCCAGTATCT GGCTTGGCCT 
GACCACGGTG TACCAGACGA TTCAACAGAC TTCCTGGATT TTGTGGCTCT GGTCCGGAAT 
AAACGAGCAG GGAAGGATGA GCCTGTGGTG GTCCACTGCA GTGCTGGGAT TGGTCGGACC 
GGAGTTCTCA TCACAATGGA GACAGCCATG TGTCTGATGG AGTGCAGTCA GCCGGTGTTT 
CCATTAGAAA TAGTGAGAAC CATGCGGGAT CAGAGGGCCA TGATGATTCA GACGCCTAGC 
CAGTTCAAGT TTGTGTGCGA GGCCATACTG AAAGTTTACG AGGAGGGCCT GGTCAAACCG 
CTCAAGTCCA TTCTGTACGA ATCCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Differential oxidation of protein-tyrosine phosphatases during zebrafish caudal fin regeneration.
Scientific reports7(1)8460(2017 Aug)
Wu W,Hale AJ,Lemeer S,den Hertog J

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Zebrafish as a model to study PTPs during development.
Methods (San Diego, Calif.)65(2)247-53(2014 Jan)
Paardekooper Overman J,den Hertog J

Identification and expression of the family of classical protein-tyrosine phosphatases in zebrafish.
PloS one5(9)e12573(2010 Sep)
van Eekelen M,Overvoorde J,van Rooijen C,den Hertog J

Zebrafish mosaic eyes is a novel FERM protein required for retinal lamination and retinal pigmented epithelial tight junction formation.
Current biology : CB14(8)711-7(2004 Apr)
Jensen AM,Westerfield M